LOCUS       DAJTNM010000001       141760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTNM010000001 DAJTNM010000000
VERSION     DAJTNM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 141760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 141760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..141760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.001"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO172_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO172_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5510336.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO172_000002"
     CDS             complement(1672..2730)
                     /locus_tag="OO172_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5510337.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO172_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO172_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO172_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO172_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5510338.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO172_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO172_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5510339.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO172_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO172_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5510340.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO172_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO172_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5510341.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO172_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO172_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5510342.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO172_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO172_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5510343.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO172_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO172_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5510344.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFCCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO172_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO172_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5510345.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO172_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO172_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5510346.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO172_000013"
     CDS             complement(17699..18322)
                     /locus_tag="OO172_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5510347.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO172_000014"
     CDS             18619..18894
                     /locus_tag="OO172_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510348.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO172_000015"
     CDS             complement(18971..19213)
                     /locus_tag="OO172_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5510349.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO172_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO172_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5510350.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO172_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO172_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5510351.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22204)
                     /gene="alaC"
                     /locus_tag="OO172_000018"
                     /pseudo
     CDS             complement(20977..22204)
                     /gene="alaC"
                     /locus_tag="OO172_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
     gene            22581..24278
                     /gene="ypdA"
                     /locus_tag="OO172_000019"
     CDS             22581..24278
                     /gene="ypdA"
                     /locus_tag="OO172_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR5510352.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24293..25026
                     /gene="ypdB"
                     /locus_tag="OO172_000020"
                     /pseudo
     CDS             24293..25026
                     /gene="ypdB"
                     /locus_tag="OO172_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
     gene            25039..25896
                     /locus_tag="OO172_000021"
     CDS             25039..25896
                     /locus_tag="OO172_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5510353.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25899..28400)
                     /gene="ptsP"
                     /locus_tag="OO172_000022"
     CDS             complement(25899..28400)
                     /gene="ptsP"
                     /locus_tag="OO172_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5510354.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDLSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28425..29462)
                     /gene="ypdE"
                     /locus_tag="OO172_000023"
     CDS             complement(28425..29462)
                     /gene="ypdE"
                     /locus_tag="OO172_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5510355.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29462..30545)
                     /gene="ypdF"
                     /locus_tag="OO172_000024"
                     /pseudo
     CDS             complement(29462..30545)
                     /gene="ypdF"
                     /locus_tag="OO172_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30560..31807)
                     /locus_tag="OO172_000025"
     CDS             complement(30560..31807)
                     /locus_tag="OO172_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5510356.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31829..32155)
                     /locus_tag="OO172_000026"
     CDS             complement(31829..32155)
                     /locus_tag="OO172_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5510357.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32374..33339)
                     /gene="glk"
                     /locus_tag="OO172_000027"
     CDS             complement(32374..33339)
                     /gene="glk"
                     /locus_tag="OO172_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5510358.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33543..34799
                     /locus_tag="OO172_000028"
     CDS             33543..34799
                     /locus_tag="OO172_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5510359.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34914..35240
                     /locus_tag="OO172_000029"
     CDS             34914..35240
                     /locus_tag="OO172_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5510360.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35381..36619)
                     /locus_tag="OO172_000030"
     CDS             complement(35381..36619)
                     /locus_tag="OO172_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5510361.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36955..38157
                     /gene="nupC"
                     /locus_tag="OO172_000031"
     CDS             36955..38157
                     /gene="nupC"
                     /locus_tag="OO172_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5510362.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38207..40396)
                     /locus_tag="OO172_000032"
                     /pseudo
     CDS             complement(38207..40396)
                     /locus_tag="OO172_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40604..40679)
                     /locus_tag="OO172_000033"
     tRNA            complement(40604..40679)
                     /locus_tag="OO172_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40644..40646),aa:Ala,seq:ggc)
     gene            complement(40719..40794)
                     /locus_tag="OO172_000034"
     tRNA            complement(40719..40794)
                     /locus_tag="OO172_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40759..40761),aa:Ala,seq:ggc)
     gene            41015..41374
                     /locus_tag="OO172_000035"
     CDS             41015..41374
                     /locus_tag="OO172_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5510363.1"
                     /translation="MSKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41376..41768
                     /locus_tag="OO172_000036"
     CDS             41376..41768
                     /locus_tag="OO172_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5510364.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41820..43235)
                     /gene="gltX"
                     /locus_tag="OO172_000037"
     CDS             complement(41820..43235)
                     /gene="gltX"
                     /locus_tag="OO172_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5510365.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43494..43569
                     /locus_tag="OO172_000038"
     tRNA            43494..43569
                     /locus_tag="OO172_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43527..43529,aa:Val,seq:tac)
     gene            43614..43689
                     /locus_tag="OO172_000039"
     tRNA            43614..43689
                     /locus_tag="OO172_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43647..43649,aa:Val,seq:tac)
     gene            43694..43769
                     /locus_tag="OO172_000040"
     tRNA            43694..43769
                     /locus_tag="OO172_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43727..43729,aa:Lys,seq:ttt)
     gene            43800..43925
                     /locus_tag="OO172_000041"
     ncRNA           43800..43925
                     /ncRNA_class="other"
                     /locus_tag="OO172_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            44045..44386
                     /locus_tag="OO172_000042"
     CDS             44045..44386
                     /locus_tag="OO172_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5510366.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44377..45303)
                     /locus_tag="OO172_000043"
     CDS             complement(44377..45303)
                     /locus_tag="OO172_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5510367.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45393..46391
                     /locus_tag="OO172_000044"
     CDS             45393..46391
                     /locus_tag="OO172_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5510368.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46388..46606)
                     /locus_tag="OO172_000045"
     CDS             complement(46388..46606)
                     /locus_tag="OO172_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5510369.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46608..48623)
                     /gene="ligA"
                     /locus_tag="OO172_000046"
     CDS             complement(46608..48623)
                     /gene="ligA"
                     /locus_tag="OO172_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5510370.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48694..49581)
                     /gene="zipA"
                     /locus_tag="OO172_000047"
     CDS             complement(48694..49581)
                     /gene="zipA"
                     /locus_tag="OO172_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5510371.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRSMMT
                     PQKLREYQDIIREVKDANA"
     gene            49811..50572
                     /gene="cysZ"
                     /locus_tag="OO172_000048"
     CDS             49811..50572
                     /gene="cysZ"
                     /locus_tag="OO172_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5510372.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50757..51728
                     /gene="cysK"
                     /locus_tag="OO172_000049"
     CDS             50757..51728
                     /gene="cysK"
                     /locus_tag="OO172_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5510373.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52112..52369
                     /gene="ptsH"
                     /locus_tag="OO172_000050"
     CDS             52112..52369
                     /gene="ptsH"
                     /locus_tag="OO172_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5510374.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52414..54141
                     /gene="ptsI"
                     /locus_tag="OO172_000051"
     CDS             52414..54141
                     /gene="ptsI"
                     /locus_tag="OO172_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5510375.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54182..54691
                     /gene="crr"
                     /locus_tag="OO172_000052"
     CDS             54182..54691
                     /gene="crr"
                     /locus_tag="OO172_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5510376.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54733..55584)
                     /gene="pdxK"
                     /locus_tag="OO172_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54733..55584)
                     /gene="pdxK"
                     /locus_tag="OO172_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5510377.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55689..56063
                     /locus_tag="OO172_000054"
     CDS             55689..56063
                     /locus_tag="OO172_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5510378.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            56096..56819
                     /locus_tag="OO172_000055"
                     /pseudo
     CDS             56096..56819
                     /locus_tag="OO172_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(57023..57934)
                     /gene="cysM"
                     /locus_tag="OO172_000056"
     CDS             complement(57023..57934)
                     /gene="cysM"
                     /locus_tag="OO172_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5510379.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MHAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(58068..59165)
                     /gene="cysA"
                     /locus_tag="OO172_000057"
     CDS             complement(58068..59165)
                     /gene="cysA"
                     /locus_tag="OO172_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5510380.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(59155..60030)
                     /gene="cysW"
                     /locus_tag="OO172_000058"
     CDS             complement(59155..60030)
                     /gene="cysW"
                     /locus_tag="OO172_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5510381.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(60030..60863)
                     /gene="cysT"
                     /locus_tag="OO172_000059"
     CDS             complement(60030..60863)
                     /gene="cysT"
                     /locus_tag="OO172_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5510382.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60863..61879)
                     /gene="cysP"
                     /locus_tag="OO172_000060"
     CDS             complement(60863..61879)
                     /gene="cysP"
                     /locus_tag="OO172_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5510383.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62183..62974)
                     /gene="ucpA"
                     /locus_tag="OO172_000061"
     CDS             complement(62183..62974)
                     /gene="ucpA"
                     /locus_tag="OO172_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5510384.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(63161..63961)
                     /gene="murR"
                     /locus_tag="OO172_000062"
     CDS             complement(63161..63961)
                     /gene="murR"
                     /locus_tag="OO172_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5510385.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            64125..65021
                     /gene="murQ"
                     /locus_tag="OO172_000063"
     CDS             64125..65021
                     /gene="murQ"
                     /locus_tag="OO172_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5510386.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            65025..66449
                     /gene="murP"
                     /locus_tag="OO172_000064"
     CDS             65025..66449
                     /gene="murP"
                     /locus_tag="OO172_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5510387.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66629..67528)
                     /gene="yfeX"
                     /locus_tag="OO172_000065"
     CDS             complement(66629..67528)
                     /gene="yfeX"
                     /locus_tag="OO172_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5510388.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(67624..68199)
                     /locus_tag="OO172_000066"
     CDS             complement(67624..68199)
                     /locus_tag="OO172_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5510389.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68260..68709)
                     /locus_tag="OO172_000067"
     CDS             complement(68260..68709)
                     /locus_tag="OO172_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5510390.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68696..69121)
                     /locus_tag="OO172_000068"
     CDS             complement(68696..69121)
                     /locus_tag="OO172_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5510391.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69335..70204
                     /gene="amiA"
                     /locus_tag="OO172_000069"
     CDS             69335..70204
                     /gene="amiA"
                     /locus_tag="OO172_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5510392.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70208..71107
                     /gene="hemF"
                     /locus_tag="OO172_000070"
     CDS             70208..71107
                     /gene="hemF"
                     /locus_tag="OO172_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5510393.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71041..72165)
                     /gene="eutR"
                     /locus_tag="OO172_000071"
     CDS             complement(71041..72165)
                     /gene="eutR"
                     /locus_tag="OO172_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR5510394.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITCQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSFG"
     gene            complement(72250..72711)
                     /gene="eutK"
                     /locus_tag="OO172_000072"
     CDS             complement(72250..72711)
                     /gene="eutK"
                     /locus_tag="OO172_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5510395.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(72724..73383)
                     /gene="eutL"
                     /locus_tag="OO172_000073"
     CDS             complement(72724..73383)
                     /gene="eutL"
                     /locus_tag="OO172_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5510396.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73393..74280)
                     /gene="eutC"
                     /locus_tag="OO172_000074"
     CDS             complement(73393..74280)
                     /gene="eutC"
                     /locus_tag="OO172_000074"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR5510397.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(74301..75662)
                     /gene="eutB"
                     /locus_tag="OO172_000075"
     CDS             complement(74301..75662)
                     /gene="eutB"
                     /locus_tag="OO172_000075"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCR5510398.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            75851..77056
                     /locus_tag="OO172_000076"
     CDS             75851..77056
                     /locus_tag="OO172_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5510399.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWINLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            77549..78313
                     /locus_tag="OO172_000077"
     CDS             77549..78313
                     /locus_tag="OO172_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5510400.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQEQEQEQEQE
                     QEQEQEQNKAAAIAEFKELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEEAE
                     EDLRFDSKEEQLPEEREQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELKLY
                     TWMVSKCRYRGNDKYEIWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALRGK
                     CGKTYRLRIPSKGTAEEASMTAQQHEREAKVSYLVH"
     gene            79364..80116
                     /locus_tag="OO172_000078"
     CDS             79364..80116
                     /locus_tag="OO172_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510401.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKQK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGYVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            80876..81271
                     /locus_tag="OO172_000079"
     CDS             80876..81271
                     /locus_tag="OO172_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5510402.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            81289..81510
                     /locus_tag="OO172_000080"
     CDS             81289..81510
                     /locus_tag="OO172_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510403.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLIC"
     gene            81575..82117
                     /locus_tag="OO172_000081"
     CDS             81575..82117
                     /locus_tag="OO172_000081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510404.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            82582..82788
                     /locus_tag="OO172_000082"
     CDS             82582..82788
                     /locus_tag="OO172_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510405.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            83100..83585
                     /locus_tag="OO172_000083"
     CDS             83100..83585
                     /locus_tag="OO172_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5510406.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            83566..83844
                     /locus_tag="OO172_000084"
     CDS             83566..83844
                     /locus_tag="OO172_000084"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510407.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            83837..85297
                     /locus_tag="OO172_000085"
     CDS             83837..85297
                     /locus_tag="OO172_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5510408.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            85331..86140
                     /gene="murI"
                     /locus_tag="OO172_000086"
     CDS             85331..86140
                     /gene="murI"
                     /locus_tag="OO172_000086"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5510409.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(86259..86950)
                     /locus_tag="OO172_000087"
     CDS             complement(join(86259..86704,86704..86950))
                     /locus_tag="OO172_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5510410.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALPPFNIGM
                     LTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEK
                     VIGAFIEKHMFY"
     gene            complement(87474..88847)
                     /gene="eutA"
                     /locus_tag="OO172_000088"
     CDS             complement(87474..88847)
                     /gene="eutA"
                     /locus_tag="OO172_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5510411.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(88822..89301)
                     /gene="eutP"
                     /locus_tag="OO172_000089"
     CDS             complement(88822..89301)
                     /gene="eutP"
                     /locus_tag="OO172_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5510412.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(89314..89649)
                     /gene="eutS"
                     /locus_tag="OO172_000090"
     CDS             complement(89314..89649)
                     /gene="eutS"
                     /locus_tag="OO172_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5510413.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(89942..92221)
                     /gene="maeB"
                     /locus_tag="OO172_000091"
     CDS             complement(89942..92221)
                     /gene="maeB"
                     /locus_tag="OO172_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5510414.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            92510..93460
                     /gene="tal"
                     /locus_tag="OO172_000092"
     CDS             92510..93460
                     /gene="tal"
                     /locus_tag="OO172_000092"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5510415.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            93480..95483
                     /gene="tkt"
                     /locus_tag="OO172_000093"
     CDS             93480..95483
                     /gene="tkt"
                     /locus_tag="OO172_000093"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5510416.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(95597..96628)
                     /locus_tag="OO172_000094"
     CDS             complement(95597..96628)
                     /locus_tag="OO172_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5510417.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQSFRLVRYAAEPTCDNW
                     HGPDARPTL"
     gene            complement(96754..97329)
                     /gene="nudK"
                     /locus_tag="OO172_000095"
     CDS             complement(96754..97329)
                     /gene="nudK"
                     /locus_tag="OO172_000095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5510418.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(97397..99376)
                     /gene="aegA"
                     /locus_tag="OO172_000096"
     CDS             complement(97397..99376)
                     /gene="aegA"
                     /locus_tag="OO172_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5510419.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDTPGVYDALPFIIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            99582..101305
                     /gene="narQ"
                     /locus_tag="OO172_000097"
                     /pseudo
     CDS             99582..101305
                     /gene="narQ"
                     /locus_tag="OO172_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            101507..104620
                     /gene="acrD"
                     /locus_tag="OO172_000098"
     CDS             101507..104620
                     /gene="acrD"
                     /locus_tag="OO172_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5510420.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNAAINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKAEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(104719..104778)
                     /gene="ypfM"
                     /locus_tag="OO172_000099"
     CDS             complement(104719..104778)
                     /gene="ypfM"
                     /locus_tag="OO172_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5510421.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            105159..105515
                     /locus_tag="OO172_000100"
     CDS             105159..105515
                     /locus_tag="OO172_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5510422.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            105519..106646
                     /gene="dapE"
                     /locus_tag="OO172_000101"
     CDS             105519..106646
                     /gene="dapE"
                     /locus_tag="OO172_000101"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5510423.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIIEQLVA"
     gene            106674..106874
                     /locus_tag="OO172_000102"
     CDS             106674..106874
                     /locus_tag="OO172_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5510424.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(106984..107682)
                     /gene="ypfH"
                     /locus_tag="OO172_000103"
     CDS             complement(106984..107682)
                     /gene="ypfH"
                     /locus_tag="OO172_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5510425.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(107756..109771)
                     /gene="tmcA"
                     /locus_tag="OO172_000104"
     CDS             complement(107756..109771)
                     /gene="tmcA"
                     /locus_tag="OO172_000104"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5510426.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAL
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGCLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(109786..110649)
                     /locus_tag="OO172_000105"
     CDS             complement(109786..110649)
                     /locus_tag="OO172_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5510427.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(110817..111530)
                     /gene="purC"
                     /locus_tag="OO172_000106"
     CDS             complement(110817..111530)
                     /gene="purC"
                     /locus_tag="OO172_000106"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5510428.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(111743..112777)
                     /gene="bamC"
                     /locus_tag="OO172_000107"
     CDS             complement(111743..112777)
                     /gene="bamC"
                     /locus_tag="OO172_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5510429.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(112794..113672)
                     /gene="dapA"
                     /locus_tag="OO172_000108"
     CDS             complement(112794..113672)
                     /gene="dapA"
                     /locus_tag="OO172_000108"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5510430.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            113818..114390
                     /locus_tag="OO172_000109"
     CDS             113818..114390
                     /locus_tag="OO172_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5510431.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            114390..114860
                     /gene="bcp"
                     /locus_tag="OO172_000110"
     CDS             114390..114860
                     /gene="bcp"
                     /locus_tag="OO172_000110"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5510432.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            115113..115730
                     /gene="hyfA"
                     /locus_tag="OO172_000111"
     CDS             115113..115730
                     /gene="hyfA"
                     /locus_tag="OO172_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5510433.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            115730..117747
                     /gene="hyfB"
                     /locus_tag="OO172_000112"
                     /pseudo
     CDS             115730..117747
                     /gene="hyfB"
                     /locus_tag="OO172_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            117758..118699
                     /gene="hyfC"
                     /locus_tag="OO172_000113"
     CDS             117758..118699
                     /gene="hyfC"
                     /locus_tag="OO172_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5510434.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGLASALLRLHGSSG"
     gene            118727..120166
                     /gene="hyfD"
                     /locus_tag="OO172_000114"
     CDS             118727..120166
                     /gene="hyfD"
                     /locus_tag="OO172_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5510435.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            120178..120828
                     /gene="hyfE"
                     /locus_tag="OO172_000115"
     CDS             120178..120828
                     /gene="hyfE"
                     /locus_tag="OO172_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5510436.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            120833..122413
                     /gene="hyfF"
                     /locus_tag="OO172_000116"
     CDS             120833..122413
                     /gene="hyfF"
                     /locus_tag="OO172_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5510437.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFFFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            122403..124117
                     /gene="hyfG"
                     /locus_tag="OO172_000117"
                     /pseudo
     CDS             122403..124117
                     /gene="hyfG"
                     /locus_tag="OO172_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            124127..124672
                     /gene="hyfH"
                     /locus_tag="OO172_000118"
     CDS             124127..124672
                     /gene="hyfH"
                     /locus_tag="OO172_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5510438.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            124669..125427
                     /gene="hyfI"
                     /locus_tag="OO172_000119"
     CDS             124669..125427
                     /gene="hyfI"
                     /locus_tag="OO172_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5510439.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            125420..125833
                     /gene="hyfJ"
                     /locus_tag="OO172_000120"
     CDS             125420..125833
                     /gene="hyfJ"
                     /locus_tag="OO172_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5510440.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            125863..127875
                     /gene="hyfR"
                     /locus_tag="OO172_000121"
                     /pseudo
     CDS             125863..127875
                     /gene="hyfR"
                     /locus_tag="OO172_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            127897..128745
                     /locus_tag="OO172_000122"
                     /pseudo
     CDS             127897..128745
                     /locus_tag="OO172_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(128783..129844)
                     /locus_tag="OO172_000123"
     CDS             complement(128783..129844)
                     /locus_tag="OO172_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5510441.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            130057..131520
                     /gene="bepA"
                     /locus_tag="OO172_000124"
     CDS             130057..131520
                     /gene="bepA"
                     /locus_tag="OO172_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5510442.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSNLLDEWAKGNVLQQRAAQYGRALQAMKANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            131541..131900
                     /gene="arsC"
                     /locus_tag="OO172_000125"
     CDS             131541..131900
                     /gene="arsC"
                     /locus_tag="OO172_000125"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5510443.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(132033..132779)
                     /gene="hda"
                     /locus_tag="OO172_000126"
     CDS             complement(132033..132779)
                     /gene="hda"
                     /locus_tag="OO172_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5510444.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(132829..134118)
                     /gene="uraA"
                     /locus_tag="OO172_000127"
     CDS             complement(132829..134118)
                     /gene="uraA"
                     /locus_tag="OO172_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5510445.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(134204..134830)
                     /gene="upp"
                     /locus_tag="OO172_000128"
     CDS             complement(134204..134830)
                     /gene="upp"
                     /locus_tag="OO172_000128"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5510446.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            135155..136192
                     /gene="purM"
                     /locus_tag="OO172_000129"
     CDS             135155..136192
                     /gene="purM"
                     /locus_tag="OO172_000129"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5510447.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            136192..136830
                     /gene="purN"
                     /locus_tag="OO172_000130"
     CDS             136192..136830
                     /gene="purN"
                     /locus_tag="OO172_000130"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5510448.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            137002..139068
                     /gene="ppk1"
                     /locus_tag="OO172_000131"
     CDS             137002..139068
                     /gene="ppk1"
                     /locus_tag="OO172_000131"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5510449.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            139073..140614
                     /gene="ppx"
                     /locus_tag="OO172_000132"
     CDS             139073..140614
                     /gene="ppx"
                     /locus_tag="OO172_000132"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5510450.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPSTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(140653..>141760)
                     /gene="pdeF"
                     /locus_tag="OO172_000133"
     CDS             complement(140653..>141760)
                     /gene="pdeF"
                     /locus_tag="OO172_000133"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5510451.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        35399 a        36529 c        34908 g        34924 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgttgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagtggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaacaaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggtag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccctc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aatgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata ccccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ccgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgag atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggtgtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccccggcga gacacaagcc ttcgcttcgt taaggagctt cttggcaaat tccagcgatc
    21181 ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc ttcggcattt
    21241 cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg cgtttgtact
    21301 gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc gccgccacct
    21361 gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg gcactgacca
    21421 gcgttttgtt gccaaccata aagccgatac gccagcccgc catgttgtag cttttcgaca
    21481 gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt gacggcgctt
    21541 tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc acatcgtagc
    21601 gtttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc gcggttgggt
    21661 tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga atggcgcgct
    21721 ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc tgcgcccctg
    21781 caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc accgtgtcac
    21841 catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca atagtgacga
    21901 tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag cgggaaatgg
    21961 cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc gggcgctggg
    22021 ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg ttacccatgc
    22081 tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca gcggtgatgt
    22141 taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg cgagtgtcag
    22201 ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct gccacaatgg
    22261 tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag aacattttta
    22321 tcaatggatt taaatgagga actccacttc aaaaacagaa aatttttcta cgctaacaag
    22381 aaaattacca ccaattttat taacattata atttcaataa atttaccagc cagaagtgca
    22441 ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt aaatttatgg
    22501 tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca tagaggttta
    22561 atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg gtcttcgatc
    22621 gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg tttcgcgaac
    22681 tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc attttctcgc
    22741 tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg gtgaacgtac
    22801 gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc atcattaccg
    22861 gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg gcgatcccct
    22921 gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg aaaatcccca
    22981 aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt gtgtgagacg ttgaccatga
    23041 ttctggtaat tgtctgggca ccaactaccg cgttggggat cgatatcgtc tctaaaatcg
    23101 gcattccaat gatcctcggt agcgtctgta tcggctttat tgtgcttctg gtgcaaagcg
    23161 ttgagggcga aaaagaggcc agcgccgcgc ggcaggccaa gctggcgctg gatatcgcca
    23221 acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag gtctgcgaaa
    23281 ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat catgtgctgg
    23341 cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt agcccgacta
    23401 cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca
    23461 caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa
    23521 cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag gaaatggcgg
    23581 tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag cagctacgtg
    23641 aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc cattttctgt
    23701 ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt
    23761 tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac gatgagcaaa
    23821 tcgatatcaa aaaagagctg tatcaaatta aagactatat tgccattgag caggcccgct
    23881 ttggcgacaa gctgacggtt atctatgata ttgatgaaga ggtgaattgc tgcattccca
    23941 gcctgctgat ccagccgttg gtggagaacg ccattgtcca cggtattcag ccttgcaaag
    24001 gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc
    24061 gagataccgg gcacggcatc gatccaaagg tgattgagcg ggtcgaagcg aatgaaatgc
    24121 cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg
    24181 ggctgcatat ccgccgcctg gagccgggga cggaaattgc gttttacatt cctaaccaac
    24241 gcaccccagt cgcctcacag gctacgttat tgctttgagc cggagtgata ttgtgaaagt
    24301 catcattgtt gaagacgaat tcctggcaca acaggaactg agctggctaa ttaaagagca
    24361 cagccagatg gagattgtcg gcacctttga cgacggtctg gacgtgttga agtttttgca
    24421 gcataaccgc gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt
    24481 gctggcgcaa aacatcagcc agttcgccca taaaccgttt attgtgttca tcaccgcgtg
    24541 gaaagaacat gcggtagaag cgtttgaact ggaggcgttt gactacattc tcaaaccata
    24601 tcaggagtca cgtattaccg ggatgctgca aaaactggaa gcggcctggc aacaacagca
    24661 gaccagcgta cgcctgccgc gacggtaacg cgtgagaatg acaccattaa tctggtgaaa
    24721 gatgagcgaa taatcgtcac gccaattaac gatatctatt acgccgaagc gcacgagaaa
    24781 atgacctttg tctatacgcg gcgtgaatcc tacgtaatgc cgatgaacat taccgaattt
    24841 tgcagcaaac tgccgccgtc gcattttttc cgctgccatc gctcattttg tgtcaatctg
    24901 aacaaaatac gcgaaatcga accgtggttt aataacacct acattctgcg actgaaagat
    24961 ctggattttg aagtgccggt cagccgcagc aaagtgaaag aatttcgcca gttaatgcat
    25021 ctttaatcgg gaactttcat gaaagcacca ggcttgcccg ccgatcaaca atttttcgcc
    25081 gatctgttca gcggcctagt gcttaacccg caactactgg ggcgcgtctg gtttgccagc
    25141 cagcctgcct cgttaccggt gggcagttta tgtattgatt ttccccgtct ggatatcgtg
    25201 ctgcgcggcg aatacggcaa cctgctggaa gcaaagcagc agcgaatggt ggaaggagaa
    25261 atgctgttta ttccggcacg cgcggctaat ttaccgatca acaacaaacc ggtgatgctg
    25321 ttaagcctgg tgttcgcacc gacctggctt gggttatcgt tttacgatag ccgcaccacg
    25381 tcgttgttgc atcctgctcg ccagatccag cttcccagcc tgcaacgcgg tgaaggtgaa
    25441 gcgatgctta ccgccctcac ccatcttagc cgttcgccgc tggagcagaa tatcattcag
    25501 ccactggtgt taagtttgct gcatctttgc cgcaacgtgg tgaatatgcc gccgggtaat
    25561 tcgcaaccgc gcggcgattt tctctatcac agcatttgta actgggttca ggataattat
    25621 gcccagccgc tcacccgcga gagcgtggcg cagtttttta atatcacgcc caatcatctg
    25681 tcaaaactgt ttgctcagca tggaacgatg cgttttatcg agtatgtgcg ttgggtgcga
    25741 atggcgaagg cgaggatgat tttgcagaaa tatcatctgt caattcatga agtggcacag
    25801 cgttgcggtt ttccggatag cgactatttt tgtcgcgttt tccggcgtca gtttggtctg
    25861 acgccgggag agtacagcgc ccgttttcag ggctaacgtc agaaggttaa ttctgtttcc
    25921 agcagcgtca ggatactttg tgcatcttgc gcggcaaaca acgactggcg gaagttttta
    25981 ttcaccagtt tacgcgccag ctgcgagaag actttcacat gattcatccc ttcgttagca
    26041 cccagcgtca gcatgatcac cagttcgact tcgcccattt ctgactgcca gtcaaccggt
    26101 ttcgccagcc gggcaatgct gatactggaa tgacggatcc actgagattt ggtgtgcggg
    26161 atcgctacgc caaaaccaac gccggtggta acaatctctt cccgctgcca gacatcttct
    26221 tccagctcaa acggatgttc agtgcgcccg ttaacgccga ggttgccgca caggaactgg
    26281 atcgcctgct ctttattgct aagatcctga tcaacaaaga tattctccag cgccagcagt
    26341 gggcgaacgt cttcttccgg cgtaaaggcg gtgagtaacg cttcaatttc ctgcgcactg
    26401 cggcattcac atgcctgacg cgccagttcc cgacacgcct cgctatccag ttgacgaagc
    26461 tggcttttca ccgccggaat acgcgggcta ctcatactca gctcatccag gcccagccca
    26521 agcagtagcg gcagataacg gctttcaccg cccagttcac cgcaaatgcc tacccatttg
    26581 ccccgctgat gcgcagtggt aactatttgc tgcaacatgc gcaggaacga tggcgtaatc
    26641 gggttatata gcggcgatac gcgcgggtta ttacgatcga ccgcatacag atactgggtc
    26701 atatcgttgg agccgatact gaagaaatcg acctcatcgc agaagtggtc gatgatgtag
    26761 cacaccgacg gcacttccac catgatccca agcgtaatcg tctctgcatg acgcaggcca
    26821 tcgcgcttaa gctcaacgat cgctttttgg atctcgcctt tcacccataa gatctgatcg
    26881 aggctgtgaa ccatcgggat catcaactgg gcgttgccga aactggcggc gcgcaaaatg
    26941 gcccgcagtt gagtgcggaa caggccagca aattccgggt aaatacgtac cgcgcgatag
    27001 ccgaggaatg ggttctcttc ctggggaaca ttcagataag aaatgctttt atcgccgccg
    27061 atgtccatcg tgcggaagat aatcggcttg tcgcccgccg ccagtagcac ctgctggtag
    27121 gcttcaaatt gctcctgctc gtccggcgcg ctgtcgcgat ccatatagag catttcggta
    27181 cggaacagcc cgacaccttc cgcgccgttg gcaaacgcgc ctggcgcttc cagagcggtg
    27241 ccgatattcg ccgcaatatc aatacgcttg ttatcacggg aataggccag ctgcgcggca
    27301 gcctgcgcct gttgtttttg gcgtttatcc gccagcgtct gcgcgacctg ataataaccg
    27361 ctcaccgcgt cattcgggtt aatcgccagc acgccgcact gggcgtcaag cactgcaggt
    27421 tgcccggcat aacgggcaat cgcatccagc ggcaagccac tcagtaccgg gatcgccgag
    27481 gcacgggcca gaatcagtgt atgcgaggtg cgcccggttt tctccagaat catacccgca
    27541 agatttttca aatcaaggct caaaaactgg cttggggtta aatcttcagc caccagaatg
    27601 gtcggttttt ccagcaccag attgttgcgc ggcttcagtt ccggccaggt gatatgcagc
    27661 aactgttcgc tgatatcgcg aatgtcgctg acccgctcgc gcagataatc gctggcagaa
    27721 gcagatagtt tggcgcaaac ctgctccata ttgctgatga tcgccgcccc cagtccctga
    27781 tgctgttctg tcatcaggcg acggatattg cctgcaaatt catcatcctg aatcagcgac
    27841 aaatgagcgc tgaggatagt tttgctttcg ccgtcacgct cacgcaattg ctgattcagt
    27901 tgctcggcaa gcgttgccag gctgtgctcc agccgggtgg aatcttgcgc actggcgggg
    27961 attgcccgat aactgtcgag gctgtcgctt tgtaacaggg tcagcgtacc cacgccgacg
    28021 ccgcttgcca gcacattgcc gtacagtaaa tccgggttaa ggcggcttaa tgaacgcggc
    28081 agcggatgcg ccgtcagttc tgcctgcgtc ggctgaacgc tgtcgctgtc gataaagcgc
    28141 accaggatgt actcttccag cacgcgccgc gcctgctctt catcgctgcc gctaatgttc
    28201 aggctgcaac tgtcattaaa taaggtgcca gttccaatca acgccagcga gcttttagca
    28261 tcggcctttg cgttctggcg atggttgata aaggtgattt cgctttgcca ctggctgcac
    28321 tgttctttaa gttcccgcgc cggacgagca tgtagaccgt taggcatagg caaaggacag
    28381 agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct gaaatccgtc
    28441 agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag ttgctccatc
    28501 tgcaaaatgt cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg ggtggcaggc
    28561 cccattacca atgtgggtac gccagtaccg gttaagtgca ccgctccacc gtccgtgccg
    28621 ccgttactga acatatccgc ctgcaacgga acgccaattt ctgccgccac ggtttcaatc
    28681 cacgcggtaa gttttggcgg cgcaatcagc gatttatcgc ttaacaccag catcggcccg
    28741 ttaccaatct gacgatggtt agccgcgcca taatcaaagt ttttcgccca gcaggcggta
    28801 tcaagcacaa tggcgacgtc cggcgacacc gcgcgggtag cagtttgccc gccgcgtaat
    28861 cccacctctt cgctggaact tgccaccagc cacacttccg caggcagttc ggcgtcgtgc
    28921 agctcacgta gtaacgtcac cagcagatag caaccgaggc gatcatcaaa ggctttgccc
    28981 atcacccgct ggtgagggag gacctgaaaa gtggtatcga aggtgacgcg atcgcctgga
    29041 cgaattcccg cctgcatcac ttcgtcatag gtgcgcgcgc caatgtccac gcgcatggcg
    29101 ctaacgtcat tcccctgccg gtcgccgtca agcaggccta gaattttgca ctcttcacgg
    29161 gtggtgatgc gcaccggctg cagctggcgg gcagccatgc gcacgttgcc aaccggcagc
    29221 acgtcaatag cgccttcgcg ggagatgctg cgcaccataa atcccacttc gtccatatgg
    29281 gcgcagatca tcaccttcgg acctgtcgat tcattgaggc ggatcagcac cgatcccaga
    29341 ccatcaaatc gcacttcttt ttgcaggcga gccgcttctt ccagcaggat ctgccgcact
    29401 tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg cttttaatag cgataaatcc
    29461 attatgcctc tcccgtgagc aacactgttt tcggcatggc gtagagcact tctgcgcctt
    29521 gcggggtaac cagcacaaca tcttcgatgc gcacgccccc ttgccctagc aaataaatcc
    29581 ccggctccac ggtcagcaac atgcctggct gtagcgtcgt ggtgtcccgc ggtgaaaaac
    29641 gcggatcttc atggacttca atgccgatag cgtgaccagt gttatgaccg aaatagtcgc
    29701 caaatccaga ctcggtaatg acccggcgcg cggcgtcgtc aatctgctgg cagcgcacgc
    29761 cggggcgaat tgcagagatt gccgcgagct gtgcctgcag gacaatctga tagacgtcaa
    29821 acagcgggtg agattcggcg ctcacccctt cgccattcac cagcaaggtg cgcgtcatat
    29881 cagcagtagc cctgatacag cgcaccaaaa tcgagagtga caaactcgcc cgctgcaaca
    29941 atcttgtcgc tggctttgcc gtgcggcagc gccccgcgcc agccgctggc gacaatggtg
    30001 tcaaaagagg ctttttctgc gccctgctgg cgcataaacc actccagttc agcggctatc
    30061 tcgcgctcgc tcatccccgc ctgaataaag cggcgaatat gctccgctcc gcaatcggca
    30121 atcccacagg cgaggcggat tatctccacc tcctctggcg ttttgatttg ccgcagcaca
    30181 tccggcgtgg cgctaaccag tttcgcattg agttcagact tccagcggtg cgcggtttcc
    30241 caactcacct gctggccttc aaaaccgagc gtttgcaact gctcatcggc aatgatttga
    30301 ttgacgatag tggtaagcgt gttcgtcgcg tcaagcaaat gcagctggta gccttgcgca
    30361 cgggcttcca cctcaacgaa atagcgcgaa tctaccagaa tgtgcgcact ttcacggcta
    30421 ataaccacat agcctgatcc ggtggagatc cccagatgcg gctgtttgtt ctgccgtgag
    30481 gagagaagca ccgcatccag ttgttgcacc ttaagccagt tgcgcagcga agcgagtaat
    30541 gtcatctgga ttttccttct tacaggctat cgattaacaa tttgcctttg cggaacatca
    30601 tcagacgcag gaacaccacc atcagcgcag taatgattgc ccccagcgcg atccccgcca
    30661 tataaacgcc caggttagtg accagcggcc atgcccagat agcggattcc gggaaccatt
    30721 gcactgcgcc cagccagaca gctgcggttg agccgacaat cgcgccgacc atatatgacg
    30781 gaatggcggt gatggggctt tccagcgcaa acggaatcgc accctcacta atccccataa
    30841 aggccaggaa catcgcggtc ttaccctgcg gataaagttg agcgttgaac aggcgtttgc
    30901 cggttaaacg acggtcaata atggtcgcca gacccagacc aatcggcgga ataacgatag
    30961 cgatagaacg agcggtgacc ggtaatacgt ggtcagtggt aaagctgaag gcaacgaaac
    31021 ctgcggcttt gttgatcggg ccaccaaggt cgattgctgt cgccgcggcg atccccattg
    31081 cgtacatcag cgcgcccttc tcacctgcgg cagtcagcac agtacggata ccgccgttga
    31141 tccagccacc aaagggggtg atgacgtagt acatcgccag catgacaaaa atggcggaaa
    31201 gaatcggtag caaaaatgtg gttttgaacg ctaacaggaa atccggtaac tggattttct
    31261 ggttcatcca tttcacaagg taaccggcaa cgatagaaat aatcagcgca ccaatgaagg
    31321 tggacggcac cggcgaagag gtcgcccatt gcatagtgct gggatcgaag ttcagcagtt
    31381 gcgtcggctg ggtagacatc aacccgccga taaaaccagc cgggaacgcc agtttgccac
    31441 cgatagagtt cgccacaaat gcagcaaaca tcggaatggc aaaaccaaac aacacgccgc
    31501 cgaaggactg cgacagccag gcaaatttaa gtaaggagag gtcgaaaccg gagaatttcc
    31561 cgctattaag agcatccatg atgccgatct cagcaggaat tttaagccag ctataagcaa
    31621 tcagctggct gaaagcgagg atcacaccgc ccataattaa ggtcggcacc atgcgtgaaa
    31681 tccctgacat cacatgctgc gggagttcac cccagaagct gcttttagag gcctgcggat
    31741 tcttaactgc cgctgccgca ccggatgcgc caggcacaac ggttgcactg cgttttttaa
    31801 tggccataac aattatccct gtcgaatatt actgttgttc agaggcaatc atctcttcga
    31861 tttctttgat gatgcccgca gcgtttttaa ttgcgtcctg caaagtgatt tcataaacgt
    31921 cgcgtgattc gaaacgttcg ttatcttccg gggtaactgc cacggaatgg atgatgatgg
    31981 tcgcttcggc gatatcctgc gccgtcaggc gattctggat gccgtccgcg ccctgggttt
    32041 caattttcac ttcataaccg gcttctaccg ccgcttcttc cagcgcctga gcggccataa
    32101 aagtgtgagc caggcccatt gggcaggcac ataaggcaat cagtttctta ctcatcgtta
    32161 attcctcgtt cagtgatagt tgacgagtga aattatgaac gtgcaaaaca aatcgccgtt
    32221 accagacaaa taatgcattt actggagaat taatcccgct gtacggaagt gtgacgcagg
    32281 tcggccttgt gaggggacac aaagaaaaat tgaattaact gtatgattta aaatattatc
    32341 gggagagtta cctcccgata taacaggaag gatttacaga atgtgaccta aggtctggcg
    32401 taaatgtgca ccggaaccga gaagacccgg atagtcatgg acgatgagat acaccggaat
    32461 atcatggaca tattctttaa agcgcccttt atcttcaaat gcggcacgga aaccggaggc
    32521 tttgaagaac tcaaggaagc gcggcacgat accgcccgca ataaacacgc cgccaaatgt
    32581 cccgagattg agcgccagat tgccgccaaa acggcccata atgacgcaaa acagcgacaa
    32641 tgcgcggcgg caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat cttttggctt
    32701 gagattttct ggcaggcggt tgtcagcttt cacaattgcg cgatacaaat tcaccagccc
    32761 agggccagaa agcacgcgct ccgccgaaac atgaccaatt tccgcacgca atatttcgag
    32821 gataatggcc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt cgcccggcaa
    32881 gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccccg ttccggcacc
    32941 gtaaaccgca ataggcttac cttcgaccgg ttctgcgcca ccaaactgaa tcagatgctc
    33001 ttttttcagc atcgggatcg ccatcgatac agcggtaaaa tcgttaataa tttccagatg
    33061 gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat ggttggtcat
    33121 tgccacccag tcaccggtaa ttgggcaagc gatggcaata cagccgtctt tcacctcgac
    33181 cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat caagccctga
    33241 ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa gacgtgcgtt
    33301 ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc tccgctaaag
    33361 tcaaaataat tctttctcac actgtaaata cttgggggca taacaacaac gccggaaagg
    33421 caggctccct gtaaatatcg atctgggtca cacaattact ttatcgtttc agcaccaatt
    33481 gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat aaggaataga
    33541 acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc gtggcaattg
    33601 ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta ttacagaatt
    33661 tgctctggca acgactgccg ggaactctgg ggatagccca ggattccccc ctctggatca
    33721 tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc agccagggtc
    33781 atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc accggttccg ccctctgcgc
    33841 tacctggact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg
    33901 aacatccgat catgaccgtc aatatcgccc ttgccgtcgc cattggcgct cgtctgttac
    33961 cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaaccatc ggcgcgctgt
    34021 ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc agtagtgaag
    34081 ttccgctatg ggatcgtctt tttgcgccgt taatggcggc agcagctggt gcacttacta
    34141 ccggattatt tttccatccc catttttcac tgcccattgc gcattacgga cagatggaaa
    34201 tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca gggatggtcg
    34261 ccgtatggtg cttaccacgg ttgcacgcga taatgaatca aatgaaaaat ccggtgctcg
    34321 tgctgggtat tggcggattt attctcggaa ttctgggggt tattggtgga ccagtttcgc
    34381 tgtttaaagg gctggatgag atgcagcaga tggtggcaaa tcaggctttc agcaccagcg
    34441 attacttttt gctggcggta attaaacttg ccgccctggt cgttgctgcc gccagtggct
    34501 ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg ttgatgctgc
    34561 atgagcacgt tcccgccgtt ccagcggcaa taaccgtttc ctgcgctatt ctcggcatcg
    34621 tgctggtggt aacacgcgat ggctggttaa gtctttttat ggcggcagtc gttgtaccca
    34681 ataccacatt gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg ttattagcag
    34741 gtaagccgat gatgatggtc aatcgtccga agcaacagcc accccacgat aatgtttagc
    34801 aaaaaatgct ctcctttatg atttaagagt tatggcgatg atacggggct ttacctcccc
    34861 gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg aaggagaata acaatgttca
    34921 ggtcactgtt tctggcggcc gccctgatgg catttacccc gcttgcagca aatgcaggtg
    34981 aaatcaccct actgccatcg atcaaattac aaattggcga tcgcgatcat tacggtaatt
    35041 actgggacgg tggtcactgg cgagatcgtg attactggca tcgcaattat gaatggcgca
    35101 aaaaccgctg gtggcgtcat gataatggct atcatcgtgg ctgggataag cgtaaagcat
    35161 atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga aaaggccgcg
    35221 gacatgggca ccgccattaa acgcgtcctc caatggagca caatgcctga tgcgacgcta
    35281 gcgcgtctta tcaggcctac aaaccggctc tttaacgcgt agggcggata aggcgtttac
    35341 gccgcatccg ccaatagtgc cagatacgac gctcattcaa ctacaatccc agcgccgtcc
    35401 ccaccaacaa ccagatattc agcgccacga ccagcactac aatcacccag cctgtctgtt
    35461 ttacgcgttt gctgttcacc agatcgccca tcaacttgct gtcactggtg aaaatcagca
    35521 gtggaaccag cgccagggcg ataccaaaac ttaacagcac ctgactcata accagaatcc
    35581 gtgtcggatc taatcccatc agaatgacaa taaatgacgg caacatggtg actgtacgac
    35641 gcacccacag cgggatatgg aagcgaatga atccctgcat caccacctgc cccgccagtg
    35701 tccccaccac cgttgaggac agtccggcag caaccagact taacccaaag accgttgccg
    35761 cagcatggct taacagcggt tgcagcgtca gataagcctc atcaagatcg gcaacaccag
    35821 tatgaccaga aaagtggaac gccgccgcag ctgtagccat catcgccaga ttgacaaaac
    35881 cggcaatcgt catggcgata gccacatccc atttggtggc ggaataacgt tgttgacgcg
    35941 aaccgccatg taaatgctga gtgagcgaag agtgcaaata aatcacatgc ggcataatcg
    36001 tcgcccctaa cacgcctgct gccaggaaga ccgcctccga agtaggtaaa ctcgggatca
    36061 ccattccttt acccagctgc gccaggttag gctgggagaa aatcaactcg acaatgtaag
    36121 ccgcggcaac aaacaacagt aacccgccaa tcactttctc cagcggtttt tgcccgcgac
    36181 gttgcagcat taaaatcagg aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg
    36241 aaacaccaag aatgagttta aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg
    36301 ccatcgcaat aatttctgcc tgaacccaat agaaccacac tacgggacgc ggatagtgat
    36361 cacgaatctg ctccgccaga tttttaccgg tggcaatccc tagtttggca gagaggatct
    36421 gaattagcat cgccatcagg ttggcccaaa cgacaaccca cagtagctga tagccgaagc
    36481 tagcacccgc ctgaatattg gtcgcaaagt taccgggatc gatataacca atcgccgcaa
    36541 tgaacgcagg tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg ctgctactct
    36601 caacgcgata gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg tttcatgcta
    36661 ttctaaacga gaatgattat caaattcatt taaatggatt gtggtgattt ctctgataga
    36721 ccaagattac gattacgaaa agattgatga cttgaatgtt atggttatgt ttaatgttag
    36781 cacatttaca taactttcag cttccataca caacatagca gaaatgtatg acagatcact
    36841 atttttgaag cctgtcacag gacgtcatta tagtgtgtgt cagatctcgt tttccttaac
    36901 catgttacat agaatgtgca cggaaattta acctgcctca tatttggagc aaatatggac
    36961 cgcgtccttc attttgtact ggcacttgcc gttgttgcga ttctcgcact gctggtaagc
    37021 agcgaccgca aaaaaattcg tatccgttat gttattcaac tgcttgttat cgaagtgtta
    37081 ctggcgtggt tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg cttctccgaa
    37141 atgttcgaaa aactactcgg atttgccaac gaagggacta acttcgtctt tggtagcatg
    37201 aatgatcaag gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt ctttatctct
    37261 gcactgatcg gtattctcca gcacattcgc gtgttgccgg tgattatccg cgcaattggt
    37321 ttcctgctct ccaaagtcaa cggtatgggc aaactggaat cctttaacgc cgtcagctcc
    37381 ctgattctgg gtcagtcaga aaactttatt gcctataaag atatcctcgg caaaatctcc
    37441 cgtaatcgta tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat gtccatcgtt
    37501 ggtgcataca tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt actgaacatg
    37561 ttcagcacct ttatcgtgct gtcgctgatc aatccttacc gtgttgatgc cagtgaagaa
    37621 aacatccaga tgtccaacct gcacgaaggt cagagcttct tcgaaatgct gggtgaatac
    37681 attctggcag gtttcaaagt tgccattatc gttgccgcga tgctgatcgg ctttatcgcc
    37741 ctgatcgccg cgctgaacgc actgtttgcc accgttactg gctggtttgg ctacagcatc
    37801 tccttccagg gcatcctggg ctacatcttc tatccgattg catgggtgat gggtgttcct
    37861 tccagtgaag cactgcaagt gggcagtatc atggcgacca aactggtttc caacgagttc
    37921 gttgcgatga tggatctgca gaaaattgct tccacgctct ctccgcgtgc tgaaggcatc
    37981 atctctgtgt tcctggtttc cttcgctaac ttctcttcaa tcgggattat cgcaggtgca
    38041 gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct gaaactggtt
    38101 tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt gctgtaaaac
    38161 catacataaa aaagccgggg atcattcccc ggctttttta cgcctgttaa tcagctaatg
    38221 gctgcgggcg accaatcaaa tacccttgca gatattgcac cccgagctta tgcaatagcg
    38281 cctgctgctg ttgcgtctcg acaaactccg cgaccacact caatgacttc gctttcgcca
    38341 gatcggtaat tgatctcaca atcatcgcat ccagcgtatt cgtgacaata tctttcacaa
    38401 agacgccatc aattttgatg atatcagcct gcaaacgctt taaccgttcg tagttggcat
    38461 atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc tgctcgatgt
    38521 tgtacatgct gctttctgcg ttagaaaacg cctgctcctc ggtgatctca agaatgaccg
    38581 cctgcgggga gatgtgataa cgcttaaaca gacgaataat ccgtccggca atattctttt
    38641 gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg tccttttttg tcgcaagggt
    38701 gtgttgccag ccacttcaac aaggattcca gcacttgcaa atcaaaacgc gcgctaaggt
    38761 taaactgagc aataaggggc agaaacttat ccggggtcat aatgccgccg tcatatttca
    38821 gtcgcgcgag gatctcatca taaccttcac cctctttgtt gcgaattggc tgggcgtaga
    38881 gcagcaaatc accctgatct aacgccgtgc gaatggtatt cagcaatagc acctgtttag
    38941 tggtctgccc ggaaaccatc tcctctctgc tatccagcgc cagcacatga tgatgtgcgc
    39001 aggattgctc cgccagccag cttaactgcc ccaacaaggg ttgcagggtt tcctgatttc
    39061 catcaaaacg cccccaggca gcaccatagc ccatatccag cccggtattg ttccagtgaa
    39121 tttgccgact attcaggatg ttaaccatat gctggagtcg cccttccgtt tccggcccgc
    39181 tcagcactaa cagcagttca ctacccggca attgatacaa cttttcgttt tcctgcatca
    39241 acggcagcag cgtacggcaa attgagcgga tacagtgaac gcgcatcatt aagccgtaat
    39301 gacgactcat aaactcaaga ttatcaatgc gcaggcagca aaaactcttg cccgcctctt
    39361 gctccggcgc ttgttccaac gcacgaaagt tgggtaggag cgttaacgga tctgttagcg
    39421 cctgcaaatg ccagcggcga ttaagccatt cactgcgatg ataaatgcgc accatataga
    39481 gcaggcaaac gctaaaggaa atcagcaccg cgagaataaa tgccagcgaa tattcggttt
    39541 ccaccccttg caaaaagttc tggttgtaat tcagaaggca aagcgttgaa acagcctagg
    39601 tgagatttaa aaacggatag cgaagattac cgaccccgag ggtaaagatg atgaagaaaa
    39661 cgggtaccag gtaaccggca ataaagtcgt tttcataagg tgtgcacaac agaagtagca
    39721 gcacgcttaa cgctgccagc cagcttaagg taaatgcgcg tttctctttg cccaacgacg
    39781 gagcgatatc cctgcgccac aatatctgcg caaagtgggg acttacaatc atgcgagtga
    39841 gatagtagaa aagcatgttg taaatcagca ctgcggtgaa aaggcttagt aaatcaacga
    39901 ccgtgaaaat ggcatccgca tcgccgaaaa aggtggatat ctttagcggg aaatcaaaga
    39961 agctccccac aagatacatg ctgcatttga tgccgatcgg cgtcaccaaa ccaagccaga
    40021 acagacgttg ccagacatat cggctggtca atccataacg ccaacgcgtc cccagctgcc
    40081 agcgtaatat tgcgcaggca cacaacacga caaacgtctg gctgccgagc atgaccgcag
    40141 tctgtaaaaa agagagatga aagttccaca gattggtgca aaacatcccg cataaaacgg
    40201 gaacgacacc gcgccagcca aagataaaca agattgacag cattacgcac agtggcatcc
    40261 acgccagaaa gatataactg gaatggataa tggcaagtgg cgaaataaaa cgggatagct
    40321 gaataagtac cacggtgagc gtaaacgcta gtgtgaatat cttgatattt tttatcaggt
    40381 tatgctccac aaacatgagt acactactct cactgatcgg ggttttcagg cacacaaata
    40441 ataagtttat tcgcatcgcg aagcaaacag gggccagcct gttgccgatt tttcatccaa
    40501 tgatgctttc accaggcggc gctaacccgg acgtaaactg tattacaaaa gcggcaaaaa
    40561 gcagagacaa aaaacccccg ctttgcagcg agggttggaa atttggtgga gctaagcggg
    40621 atcgaaccgc tgacctcttg catgccatgc aagcgctctc ccagctgagc tatagcccca
    40681 cgatgcgttt acgtaccaaa tttgctgggt gcaaaatttg gtggagctaa gcgggatcga
    40741 accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag ccccgtcacg
    40801 taaagcttgt cgagttgacg ggcggcatca tatgaattcc gcccgaatgt gtcaacggca
    40861 aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa atgaacaatt ttggaaaacc
    40921 gctcgcattc agtagttatt catgtcacgg tttcctgtaa agtggtgtta taaaatgaac
    40981 tactaataga cccacataca ttcagggaat tgttatgtcc aaggagagga tgacaccaga
    41041 tgaacttgcc agactgaccg gctatagccg ccagaccatt aataaatggg tacgcaagga
    41101 aggctggaca acgtcaccaa aacctggcgt ccagggtggt aaagcccgac tggttcacgt
    41161 caatgaacag gttcgtgaat atattcgcaa tgctgaacgt ccagaaggtc agggagaagc
    41221 gcctgccctt tctggtgatg cgccgcttga agtattgctg gtgacactgg cgaaagagat
    41281 gacgccagtt gaacaaaaac agtttacatc cttgcttctg cgggaaggga ttatcggatt
    41341 gttacaacgc ttagggattc gcgatagcaa ataatatgaa aagattacgc aataaaatga
    41401 ccaccgaaga actggctgaa tgcctcggtg tggctaaaca aactgttaac cgttggatca
    41461 gagaaaaagg ctggaaaacg gaaaaatttc ccggcgtgaa aggcggtcgt gccagactta
    41521 ttctggtcga tacgcaagtt tgcgagttta ttcagaacac gccagccttc cataacacac
    41581 caatgttgat ggaagctgaa gagagaattg ctgaatacgc tcctggagca cgcgccccgg
    41641 cgtatcgaca aatcatcaac gcgatagata atatgacaga cattgagcag gaaaaagtcg
    41701 cgcaattttt gtcacgtgaa ggaattcgca atttcctcgc ccgtctcgat attgacgaat
    41761 cagcataaat aaaaaacggc aggatattat ctcctgccgt ttatcttttt acacgctaat
    41821 tactgctgat tttcgcgttc agcaataaaa tccagcgctt tgttgatacg ctcgatactg
    41881 cgggtcttac caattgcgtg aacggtaaca tccagtgctg gagactgccc cgcaccggtt
    41941 acggcgacac gcagcggcat accaacttta cccataccca cttccagctc atccgccgtc
    42001 gcctgaatag cgtgatgaac gttttcagcg gtccagtcag taatcgcggc cagtttgtca
    42061 cgaaccactt ccagcggctg acgcgctacc ggacgcagat gttttttcgc cgcatcggca
    42121 tcgaactcag caaaatcttc gtagaaataa cggcagctct gtgccatctc tttcagcgtc
    42181 ttgcagcgtt cgcccagcag tttcaccaga tcagccagct gcgggccgtt acgggtatcg
    42241 atattttcct gctcaatgtg ccactgtaag tgagtagcaa catactccgg cggcagcgcg
    42301 ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt tgaacgcact ggcagatttg
    42361 ctgacggcat tcaaagtgaa gtatttgatc atctcttcac gagtgaagat ttcctgatcg
    42421 ccgtgggacc agcccagacg caccagatag ttcagcagtg cttctggcaa ataaccgtca
    42481 tcacgatact gcattacgct gactgccccg tgacgtttgg acagtttttt accgtcatcg
    42541 ccattgatca tagaaacgtg cgcgtaaacc ggcaccggcg ctttcagggc cttaagaatg
    42601 ttgatctggc gcggcgtgtt gttgatatgg tcttcgccac ggataacgtg ggtgatttcc
    42661 atatcccagt catcgacaac cacacagaag ttataggttg gggaaccatc ggtacggcgg
    42721 ataataagat cgtccagttc ctggttgctg aactcgatcg gaccacggat ctgatcgtca
    42781 aaaacaacag aaccttcctg cgggttagca aaacgtacaa cacacggttc atcatcagca
    42841 tgatgctcat ggctgtggcg gcagcgaccg tcataacgcg gcttctcacc tttcgccatt
    42901 tgctcttcgc gcagcgcttc caggcgctct ttagagcaat agcatttata agcagtgccc
    42961 tcttccagca tctgatcgat caccgcgttg tagcgatcaa aacgtttggt ctggtagtac
    43021 ggaccttcat cccactccag gctcagccag ttcatgccat ccataatggc ttcgatagct
    43081 tccggcgtgg aacgctcaag atcggtgtct tcaatacgca gcacgaactc accgccgtgg
    43141 ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg cagatagcct
    43201 gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt ttagaaagat
    43261 gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat attctatcac tcccgcctga
    43321 ttcctcaatg tagttcgggt ttctacctta cgcattattg ttttcgttga caaattgcgc
    43381 aacacggtct gttttgcgat catcttgctt aaatttacga cgaacgaaca atttctttag
    43441 aaaatgcgtt gactcatttt gaactctccc tataatgcga ctccacacag cgggggtgat
    43501 tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat
    43561 cacccaccaa ctactttatg tagtctccgc cgtgtagcaa gaaattgaga agtgggtgat
    43621 tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat
    43681 cacccaccac ttcgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt
    43741 cgcaggttcg aatcctgcac gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt
    43801 actatctgcg atactcaaag attcgaacct gcagcaggtt tgagttgagc gcagcgaaac
    43861 aacggagccg ctcgcggcga cggcccgaag ggcgagcgaa gcgagtcatc ctgcacgacc
    43921 caccaatgca aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgattt gtaagattga
    43981 tttttcttct caagttcctt gttcttctac cgatattacc tcgtacagtg acgaggactg
    44041 aacaatggcc actatctctt caacttcaat tccttccata caaacacaga gtagtaacag
    44101 ggcatcacaa ggtagcgatg ttgcttcaca aatcgcgcgg ataagtcagc aaatcatcaa
    44161 actcactcaa caaattaaag agattgtaga cactagcggc agtgctgaag acaagcaaaa
    44221 acaggccgaa ttgattcaac agcaaatcac cctactggaa acacaactgg cgcagttgca
    44281 gaaacagcag gcagaaaagg cgcaggagaa agagcagcgt ttgtctctga atgtatccct
    44341 tctaaatcct gttgaaaaaa cgactcatat cgatatctat atctgataca acggatcccc
    44401 ttcccgacca gccattagcg cattgccctg gtctcgtacc acgtcccata acgcttcagc
    44461 ggcggtggaa agtgaacggt tcttcctgcg caccagcatc aactggcgtt caaccaccgg
    44521 cgtaatgcgc ttaaccacca gtgggctacc ttcaggcaat ggtagcgcca gtgcaggaag
    44581 aatactgatg ccgatgcctg cggctaccat cgggaacagc gtcgccggat gtccaatctc
    44641 ctgcacaata ttcgcctgaa taccattgcg cgccagcgct gcgtcaatca gcggtcggct
    44701 gcctgacgcg taatcctgca acaccaattt cgctccctgt aatgcctgcc agggtacata
    44761 atcttccacg gccaaagcgc tatcgcgatg gcaaagcaga aagaaaggct cggaaagaat
    44821 cgcttcacat tgcagatcgc ccactgggcc tggatcgatg acgatgccaa agtcgacatc
    44881 tccctggcga atactttcca tcacccactg ctgcggacga tcatgcagga caaactgaat
    44941 atctggatag cggcgatggc tttcagcaat gcattgcgga ataagatgcg cggaaatggt
    45001 ctggctggca gcgacccgaa cttttccgct cagttgttgc cccatacgcc cggtatcgcg
    45061 caacgtgctg ttaagttcat ccagcagtcg ctcaagacgc aaagccaact gctgccctgc
    45121 atctgtaagc accacttcac gcgtggttct gtccagcagg cgaacaccag tatgattttc
    45181 cagctccttc acactgtgac ttactgccga ctggctcaag ccgatacgct ctcctgcacg
    45241 actaaaactt ttctcctgcg ctactgtgac gaaaaccttt aattgtttta aagaataatt
    45301 catctgtttt cttcatgaat ggatgcaata aatcaatttt atttctcaaa ctggaagaag
    45361 cacaatagaa ccatcgatca tctggagtct ttatgaaact ttttcgtatc ctcgatcctt
    45421 tcaccttaac cctgatcacg gtggtgttgc tggcctcttt ctttccggcc agaggcgatt
    45481 tcgtcccctt ctttgaaaat ctgaccaccg cagctattgc cctgctgttc tttatgcacg
    45541 gcgcgaagtt gtcgcgtgag gcgattattg ctggcggtgg tcactggcga ctgcatttgt
    45601 gggtaatgtg cagcaccttc gtgctgtttc cgattctggg tgtactgttt gcctggtgga
    45661 aaccggtaaa tgtcgacccg atgctctact ccggttttct ctacttgtgc attctcccgg
    45721 ctaccgtgca gtctgcaatc gccttcacgt caatggcggg cggtaacgtc gcggcggcgg
    45781 tttgttctgc gtcagcatcc agcctgctgg ggattttcct ttcaccattg ctggttggtc
    45841 tggtgatgaa cgttcacggt gcaaggggca gccttgagca ggtcggtaaa attatgctgc
    45901 aactgctgct gccgtttgtg ttggggcatc tttcccggcc gtggattggt gactgggtgt
    45961 cgcgcaataa aaaatggatt gcgaaaactg accagacgtc cattctgttg gtggtttata
    46021 cagcgttcag cgaagccgtc gttaatggta tctggcataa agttggctgg ggatcattgc
    46081 tgtttatcgt ggtggtcagc tgcgttcttc tggctatcgt gattgtagtt aacgtcttta
    46141 tggcacgccg actgggcttc aataaggcag atgaaattac tatcgtcttt tgtggttcga
    46201 aaaagagtct ggcaaatggc atcccgatgg caaacattct gttccccaca tcggtgatcg
    46261 gtatgatggt gctgcccctg atgattttcc atcagatcca attgatggtc tgtgcggtgc
    46321 tagcgcgtcg atacaaacgc cagaccgaac agttacaggc gcagcaggaa agcagcgccg
    46381 ataaagctta aagcggacgc ttcaggggct ggaccagttg cgtcagcccc tcggttttaa
    46441 tcagcagcgt gatttgcatt agctcaccga gcttccctgc cgggaactca tctttgcggg
    46501 caaaccacag cagatactcc tccggcaggt caattaagcg acgccctttg tatttgccaa
    46561 acggcattat cgtattggct atttcaatca gctgctcttt ttccatctca gctacccagc
    46621 aaacgcagca tttccgcttc gtcgatgact tcaatgccca gttcctgcgc cttcgccagt
    46681 ttagatcctg cagcttcacc cgctatcacc agatcggttt tcttcgacac gctgcccgcg
    46741 actttcgcgc ccagttcgac cagtcgagct ttagcgtcat cacgcgacat ctggcttaag
    46801 ctgcccgtaa gcaccacggt tttaccagca aacgggctgt caatctcttc cgcgttgata
    46861 acgatcggcg caggccagtg aacaccttcc gccaacagct cgctgatgac attgcggttg
    46921 ctttcttcgg caaagaagtt gtgaacgtgg gatgcaacga caatgccaac atcaggcacc
    46981 ttttgcagct cttcaatcga agcggcttcc agcgcttcca gcgtgccgaa atatgccgcc
    47041 agacctgctg cggtggcctc gccgacttca cggatgccaa gtgcatagag gaagcgagca
    47101 aaggtggttt ctttcgcttt ttccagcgcg ttaaccacgt tttgtgccaa ttttggcccc
    47161 atacgctcca gtccggtcag tttgcctgcg gtgagtttga acagatctgc cggagtatgg
    47221 acatattctt tttcaaccag ctgatcgatg attttgtcgc ccattccgtc aacatccatc
    47281 gcacggcggg aaacaaagtg tttcagcgac tctttacgct gcgcaccgca aatcaggcca
    47341 ccggtacagc gggcaaccgc ttcaccttcc acacgctcaa cgtcagaacc acataccgga
    47401 caatgcgtcg ggaatacaac ctcacgggta tcttccgggc gttcagaaag cacgacgtta
    47461 accacctgcg ggatcacgtc gccagcgcgg cgaatcacca ctttatcgcc aatgcgtaaa
    47521 ccaagacgtt cgatttcatc cgcattgtgt aaggttgcgt tactcaccag cacgccagca
    47581 acatggacag gttccagacg cgcaacaggc gtaatcgcgc cagtacgccc aacctgaaac
    47641 tcgacgtcac gcacaaaggt catctgctcc tgcgccggga atttaaacgc taccgcccag
    47701 cgcggggcac gcgcgacaaa gccaagctgc tcctgctgtt ccagtgagtt gaccttaatc
    47761 accacgccgt cgatatcaaa gcccagcgtc gggcggtctt cttccacttt gtgatagaac
    47821 gccagcactt cttccgccga ttcacaaagc gttacccgat cgctgaccgg caacccccac
    47881 tttttaaatt gcagtaaacg gccaagatga gtatccggca gctcgccccc ttccagaaca
    47941 ccaacgccat agcagaaaaa agtgagcggt cgcttcgctg taatacgcgg atcaagctga
    48001 cgcagtgaac cagctgccgc attacgtggg ttagcaaaaa ctttcccgcc cgtgcgtcgc
    48061 gcatcttcgt taatcttttc gaaccccgcc tgcggcagga acacttcacc acgcacttcc
    48121 agacgcgccg ggatattctc tccgtgcagc ttcagcggaa tggcgcgaat agtacgcaca
    48181 ttagacgtga tatcttcccc ggtggtgcca tcgccacggg tcgcggcact gactaaaacg
    48241 ccattttcat acagaatact gacggcaagg ccatccagct tcagctcaca gcaccaggtg
    48301 actttctcgt tgtttttcag acggtcctgc acacgtttgt tgaaagcaag aaagctttct
    48361 tcatcaaaaa cgttatccag tgacagcatt ggtacttcat ggcgtatctg gctgaaagct
    48421 gccagcggcg cagcacctac acgttgggta ggcgaatcag gcgtaatcag ttctggatgt
    48481 ttggtttcca gctcgcgcag ttcgcgcatc agcctgtcgt attcagcgtc gggaatttcc
    48541 ggcgcatcca tcacatgata aagatattca tgatggcaaa gcgtcgttcg cagttctgtc
    48601 agttgttgtt cgattgattc catatcgcac catcaatgct aaaaaccccc gacaagcggg
    48661 ggttcgaaga ggagttaatt tgccttaagt gtatcaggcg ttggcgtctt tgacttcgcg
    48721 gatgatgtcc tggtactcgc gcaatttctg cggagtcatc atactgcgct gatcgtcaag
    48781 cacgacaccg cccacttcat cggcaatatg ctgcgcagat tgcagcatca gcttgaagtt
    48841 ctgcagctcg tcaccgtaag acggtacctg cataaagata gtgacacccg gagtagtgaa
    48901 atccttcatt tcaggatcaa aggttcccgg tttcaccata ttcgccaggc tgaataacgc
    48961 cgggccgctg ccatccgggc taagatgacg atggtaaata ttcatatcgc caaaaatgaa
    49021 gcccgcttgt tgaatgctgt taagaagcag ttcaccgttt agctcgctac cgtgatgcgc
    49081 cgcgacgttc atgataatca ccgcttcttt gcgcttcggt ttatccataa ctggagccgg
    49141 ttccgctaca ggctcaggct gtggtgccgc tacgggttct gcaggctgga aagcctgttg
    49201 tgccggttgc ggtgctgaat gcactggctg cggcgctggc gcgacctgtg gcgaaactgg
    49261 ctgctggacc ggttgacgcg gctgcgcaga cgcataaggc ggttggtact ggtgttgcgg
    49321 cgacggacga gcagcctcat gctcctgagc gttagccggg gcatgattca cgcggtgaac
    49381 acgaacctca ccaacgccct catcatcttc gacatcctcg tcataagaat cgtcgtcacg
    49441 ttttgacttc attcgtttta atggccgatc gcggaacata gaagatcgtt ctttacggct
    49501 ggtccagaaa ccatgtacca gtaaagcgat tatggcgatc gcgccaacaa tgattaatat
    49561 cagacgcaaa tcctgcatca ttatattctc tgttgttcta acaccttgcc accacggcaa
    49621 acatttactc actaagagta tttgccgatt acctcaagtg caagtgcact attaactttc
    49681 acagcacaaa gatagatgaa atcgtgcttt ttgctgtttt ttcgaacata tcctaactgt
    49741 ccattgcgca attacccggt aaaatacgca gaattttcct gggattggtc aaaaggagct
    49801 catcctgatt atggtttcat cattcacatc tgccccacgc agcggttttt actattttgc
    49861 gcaaggctgg aagctcgtct cgcaacctgg gattcggcgt ttcgttattt taccgctgct
    49921 ggtcaatatt ttgttgatgg ggggcgcatt ctggtggctc tttacacagc tcgatgtctg
    49981 gatcccgact ttcatgagtt acgttccgga ctggctacaa tggctgagtt atctgttgtg
    50041 gcctctggct gtcatctctg tgctgttagt gtttggctat ttcttctcca cgattgctaa
    50101 ctggattgcc gctccgttta acggtttatt ggctgaacaa ctggaagcac gattgactgg
    50161 cgctacaccg ccagataccg ggattttcgg tatcatgaaa gatgtgccgc gaatcatgaa
    50221 acgcgaatgg caaaaatttg tctggtatct gccgcgcgca attgtattac taattcttta
    50281 cttcatccct ggtattgggc aaaccgtcgc gccggtactg tggttcctgt ttagcgcctg
    50341 gatgttagcc atccagtatt gcgattaccc cttcgataac cacaaagtgc cgtttaaaga
    50401 gatgcgcacc gccctgcgca cacgcaaaat caccaatatg cagtttggtg ctttaaccag
    50461 cctgtttacg atgatcccgc tgcttaatct gttcatcatg cccgttgccg tttgtggcgc
    50521 gacggcgatg tgggtcgatt gctatcgcga taaacacgcg atgtggcggt aacaatctac
    50581 cggttatttt gtaaaccgtt tgtgtgaaac aggggtggct tatgccgccc cttattccat
    50641 cttgcatgtc attatttccc ttctgtatat agatatgcta aatccttact tccgcatatt
    50701 ctctgagcgg gtatgctacc tgttgtatcc caatttcata cagttaagga caggccatga
    50761 gtaagatttt tgaagataac tcgctgacta tcggtcacac gccgctggtt cgcctgaatc
    50821 gcatcggtaa cggacgcatt ctggcgaagg tggaatctcg taaccccagc ttcagcgtta
    50881 agtgccgtat cggtgccaac atgatttggg atgccgaaaa gcgcggcgtg ctgaaaccag
    50941 gcgttgaact ggttgaaccg accagcggta ataccgggat tgcactggcc tatgtagctg
    51001 ccgctcgcgg ttacaaactc accctgacca tgccagaaac catgagtatt gaacgccgca
    51061 agctgctgaa agcgttaggt gcaaacctgg tgctgacgga aggtgctaaa ggcatgaaag
    51121 gcgcaatcca aaaagcagaa gaaattgtcg ccagcaatcc agagaaatac ctgctgctgc
    51181 aacaattcag caatccggca aaccctgaaa ttcacgaaaa aaccaccggt ccggagatct
    51241 gggaagatac cgacggtcag gttgatgtat ttattgctgg cgttgggact ggcggtacgc
    51301 tgactggcgt cagccgctac attaaaggca ccaaaggcaa gaccgatctt atctctgtcg
    51361 ccgttgagcc aaccgattct ccagttatcg cccaggtgct ggcaggtgaa gagattaaac
    51421 ctggcccgca taaaattcag ggtattggcg ctggttttat cccggctaac ctcgatctca
    51481 agctggtcga taaagtcatt ggcatcacca atgaagaagc catttctacc gcccgtcgtc
    51541 taatggaaga agaaggtatt cttgcaggta tctcttctgg agcagctgtt gccgcagcgt
    51601 tgaaactaca agaagatgaa agctttacca acaagaatat tgtggttatt ctaccatcat
    51661 cgggtgagcg ttatttaagc accgcattgt ttgccgatct cttcactgag aaagaattgc
    51721 aacagtaatg ccagcttgtt aaaaatgcgt aaaaaagcac ctttttaggt gcttttttgt
    51781 ggcctgcttc aaactttcgc ccctcctggc attgattcag cctgtcggaa ctggtattta
    51841 accagactaa ttattttgat gcgcgaaatt aatcgttaca ggaaaagcca aagctgaatc
    51901 gattttatga tttggttcaa ttcttccttt agcggcataa tgtttaatga cgtacgaaac
    51961 gtcagcggtc aacacccgcc agcaatggac tgtattgcgc tcttcgtgcg tcgcgtctgt
    52021 taaaaactgg cgctaacaat acaggctaaa gtcgaaccgc caggctagac tttagttcca
    52081 caacactaaa cctataagtt ggggaaatac aatgttccag caagaagtta ccattaccgc
    52141 tccgaacggt ctgcacaccc gccctgctgc ccagtttgta aaagaagcta agggcttcac
    52201 ttctgaaatt actgtgactt ccaacggcaa aagcgccagc gcgaaaagcc tgtttaaact
    52261 gcagactctg ggcctgactc aaggtaccgt tgtgactatc tccgcagaag gcgaagacga
    52321 gcagaaagcg gttgaacatc tggttaaact gatggcggaa ctcgagtaat ttcccgggtt
    52381 cttttaaaaa tcagtcacaa gtaaggtagg gttatgattt caggcatttt agcatccccg
    52441 ggtatcgctt tcggtaaagc tctgcttctg aaagaagacg aaattgtcat tgaccggaaa
    52501 aaaatttctg ccgaccaggt tgctcaggaa gttgaacgtt ttctgagcgg tcgtgccaag
    52561 gcatcagccc agctggaaac gatcaaaacg aaagctggtg aaacgttcgg tgaagaaaaa
    52621 gaagccatct ttgaagggca tattatgctg ctcgaagatg aggagctgga gcaggaaatc
    52681 atagccctga ttaaagataa acacatgaca gctgacgcag ctgctcatga agttatcgaa
    52741 ggtcaggctt ctgccctgga agagctggat gatgaatacc tgaaagaacg tgcggctgac
    52801 gtacgtgata tcggtaagcg cctgctgcgc aacatcctgg gcctgaagat tatcgacctg
    52861 agcgccattc aggatgaagt cattctggtt gccgctgacc tgacgccgtc cgaaaccgca
    52921 cagctgaacc tgaagaaggt gctgggtttc atcaccgacg cgggtggccg tacttcccac
    52981 acctctatca tggcgcgttc tctggaactg cctgctatcg tgggtaccgg tagcgtcacc
    53041 tctcaggtga aaaatgacga ctatctgatt ctggatgccg taaataatca ggtttacgtc
    53101 aatccaacca acgaagttat tgataaaatg cgcgctgttc aggagcaagt ggcttctgaa
    53161 aaagcagagc ttgctaaact gaaagatctg ccagctatta cgctggacgg tcaccaggta
    53221 gaagtatgcg ctaacattgg tacggttcgt gacgttgaag gtgcagagcg taacggcgct
    53281 gaaggcgttg gtctgtatcg tactgagttc ctgttcatgg accgcgacgc gctgcccact
    53341 gaagaagaac agtttgctgc ttacaaagca gtggctgaag cgtgtggctc gcaggcggtt
    53401 atcgttcgta ccatggacat cggcggcgac aaagagctgc catacatgaa cttcccgaaa
    53461 gaagagaacc cgttcctcgg ctggcgcgct atccgtatcg cgatggatcg taaagagatc
    53521 ctgcgcgatc agctccgcgc tatcctgcgt gcctcggctt tcggtaaatt gcgcattatg
    53581 ttcccgatga tcatctctgt tgaagaagtg cgtgcactgc gcaaagagat cgaaatctac
    53641 aaacaggaac tgcgcgacga aggtaaagcg tttgacgagt caattgaaat cggcgtaatg
    53701 gtggaaacac cggctgccgc aacaattgca cgtcatttag ccaaagaagt tgatttcttt
    53761 agtatcggca ccaatgattt aacgcagtac actctggcag ttgaccgtgg taatgatatg
    53821 atttcacacc tttaccagcc aatgtcaccg tccgtgctga acttgatcaa gcaagttatt
    53881 gatgcttctc atgctgaagg caaatggact ggcatgtgtg gtgagcttgc tggcgatgaa
    53941 cgtgctacac ttctgttgct ggggatgggt ctggacgaat tctctatgag cgccatttct
    54001 atcccgcgca ttaagaagat tatccgtaac acgaacttcg aagatgcgaa ggtgttagca
    54061 gagcaggctc ttgctcaacc gacaacggac gagttaatga cgctggttaa caagttcatt
    54121 gaagaaaaaa caatctgcta atccacgaga tgcggcccaa tttactgctt aggagaagat
    54181 catgggtttg ttcgataaac tgaaatctct ggtttccgac gacaaaaagg ataccggaac
    54241 tattgagatc attgctccgc tctctggcga gatcgtcaat atcgaagacg tgccggatgt
    54301 cgtttttgcg gaaaaaatcg ttggtgatgg tattgctatc aaaccaacgg gtaacaaaat
    54361 ggtcgcgcct gtagacggta ccattggtaa aatctttgaa accaatcacg cgttctctat
    54421 cgaatctgat agcggcgttg aactgttcgt ccacttcggt atcgacaccg ttgaactgaa
    54481 aggcgaaggc ttcaagcgta ttgctgaaga aggtcagcgc gtgaaagttg gcgatactgt
    54541 cattgaattt gatctgccgc tgctggaaga gaaagccaag tctaccctga ctccggttgt
    54601 tatctccaac atggacgaaa tcaaagaact gatcaaactg tccggtagcg taaccgtggg
    54661 tgaaaccccg gttatccgca tcaagaagta attcttgccg cagtgaaaaa tggcgcccat
    54721 cggcgccatt ttttatgctt ccgccagcgg cggcaaaatc aattcatcgc tctcatgctg
    54781 ctgggtgtag cgcatcactt ccagtacgcg caaccccgca cggtgcaccg catcgtttaa
    54841 cgccttccct ttcagcaaac cactgataag ctgagcacaa aacaggtcgc cagtcccttt
    54901 caggtcggtt tttacccgtg aatgggaaat gacattcacg ctgtcggcac tgaccaccac
    54961 cacctgcatc tcctgatttt cttcattacc ggaggcgctg gtaatcacca cccattttaa
    55021 tgtgtctgaa agcagacttt ttgcggcagc aatggcactg tcgagatcgc ggcaattttt
    55081 accggtcagg atttccagct caaagatatt gggggtaatt ccctgcgcca gcggcagtaa
    55141 atattgtcga tacgcctcgg gaaggtcagg tttgacataa attccgctat caatatcgcc
    55201 aatcaccgga tcgaccatga tcaataggtc aggatggtct ttgcgtagcg cagtcagcca
    55261 ctcggcaagg attttgattt gcgatgccgt tcccatatag cccgtggtta cagcacgaag
    55321 ttggcgcagc gcatcacgct cctgaagcgc acgcaaatag ccgctaaacc attcgtccgg
    55381 aatcgcacca ccgtagaaag tgtcataatg cggcgtattg ctcagcaata ccgtcggcac
    55441 ggcaaagaca ttcaagccgt tctgttttat agcaggcacc gcaatgctgt tgcctacgct
    55501 tccgtacact acctgcgact gcacggcgac gatatccgcc tgcagcgccc tactcttatc
    55561 gttaaacaac aacaaactac tcatttaatt ttttctcctt gccgatgatc ctcatcgtat
    55621 tccaaccgaa actttacctg attctggcag tcaaatcggc tattacaaaa caaagataag
    55681 gtaattcagt gaagaaaatc atttgtctgg ccattacgct gctaatgaca ctccccgctt
    55741 acgcgaagtt aactgttcat gaagaagccc gcatcaacgc catgctggag ggattagcac
    55801 agaaaaagga tttgatattt gtgcgcaacg gtgatgaaca tacctgcgat gaagcggttt
    55861 cccacctgcg tctgaagctc ggcaataccc gtaaccgcat tgacactgcc gagcagttta
    55921 ttgataaggt agcttcgtcg tcatcgatta ccgggaagcc gtatatcgtg aagatccccg
    55981 gtaagagcga tgagaacgca cagccttttt tacatgcgtt aattgcgcag acggataaaa
    56041 cggtgcctgc ggaaggaaat taatccgctt tggtaaggca tttacaggag gtaacatgaa
    56101 aaaatgcttt atttatcacg atgaaaaatc gaataaattt tggtggatag attacgaagg
    56161 ggatagttta gctgtcaact atggcaaggt aggtagtatt ggtaaattcc atacaaaaga
    56221 attcgataat gaagaacagt gtctgaaaga agccagtaaa ttgattgccg caaaaatgaa
    56281 aaaaggatat caagaaactc cagactttaa cttcatggat ctctactatt ttgatgatga
    56341 agagattggg ttacatctta aaacgtcaca cccaaacttc cagtgccatt ttactgatcc
    56401 actttatatg tgttgctggg atgaagaatc tccttttggt agcgatgaag gtgctgatgc
    56461 tctaaacgaa cttgaaaata gcctccgttc tggactgtgc tgatttccct caaatgttaa
    56521 ttgaaactat gtggggtatg aaatacatcg ctatggacag tattcttgaa gaggatgtgc
    56581 gtgcgcaatt actagccgat gaaatgagct ctatccagag caatatgatt acctacgcga
    56641 ctgcattcgg tcagatcaaa gtcatgggta aaatctccca taaacttaaa aagatgggac
    56701 tcaatgcact agcgcgtcat cagcttaccg caaaaattct tcaatggggt gacggtcagg
    56761 actcaccaat acttcaaaaa atgattgatg accttacttc ttttccacac gaaaattaaa
    56821 aactacattt gtcggcagca acaactgccg acaaatcaga ttattgacaa agtgcacttt
    56881 gtttatgccg gatgcggcgt aaacgcctga tccggcataa aaatattgct aattcaatat
    56941 attgcagaaa cattgtaggc ctgataagcg gagcgcatca ggcagttttg cgtttgtcat
    57001 cagtctccaa tgctattaat ccttaaatcc ctgccccctg actaaaatgc tcttccccaa
    57061 acactccggt ggagaggtag cgatcgccac gatcgcagat gatcgctacc accaccgcac
    57121 cagggttagc ttttgccacc cgcagtgctc cagcaaccgc gccgcctgag ctgacgccac
    57181 agaatattcc ttcccgcacc gccagttcgc gcatggtgtt ttccgcatcg cgctgatgaa
    57241 tatccagcac ctcatccacc agcgcagcgt tgaaaatccc cggcagatat tccgcaggcc
    57301 agcggcgaat gcccggaata ctgctgcctt cttccggttg caggccaaca atggtcaccg
    57361 gtttggattg ttcgcgcata aagcgtgaaa cgccggtgat agtgccggtc gtccccatgc
    57421 tggagacaaa atgagtgatg cgcccgccgg tttgctgcca gatttccggc ccggtggtgg
    57481 tgtaatgcgc ataagggtta tcgggattat tgaactgatc gagcagcttt ccttcgccac
    57541 gattcgccat ctccagcgcc agatcgcgcg caccttccat gccctgctct ttggtgacaa
    57601 gaatcagttc cgcaccataa gcatgcatcg ccgcacggcg ttcctggctc atgttgtcgg
    57661 gcatcagcaa tttcatgcga tagcctttca gcgcggcaat cattgccagc gcaatgccgg
    57721 tgttaccact ggtggcttcg attaagacat cacccggttt aatttccccg cgcttttccg
    57781 cctcgacgat catcgaaagt gccgcacgat ctttcaccga acctgccggg ttattgcctt
    57841 ccagttttaa ccacacttca ctgccgttat ccggccccat tcgctgcaac ttcaccagag
    57901 gcgtattgcc tattgtttgt tctaatgtac tcacgatctc tatccatacg tggtgttgcc
    57961 tgatgcgacg cttgcgcgtc ttatcaggcc tacaggttac aaaccttgcc attaaaaagc
    58021 ccggatcgcg ggaagcgcct ccgggcgttt aacattcact caacctatca ggcgctttgt
    58081 gcgagagcaa gttcctcatc gcgggtttca atacgctcgt cgccgttata cagccgcgca
    58141 tgttgcagac caacgaataa acgctcgcca cgctgcgggg catcgtcgcc atgcatcacg
    58201 accgtcagcg gttcgttgta ccaccccagc ggctgcacca ctaattgggt gtagtgacct
    58261 ttcgggctgg cttccagtac ctgtaccggc agcggcgaat cgagactggt acggcggctg
    58321 atatccactt cccacgggcg caggaagaga tccaccggac cctgatacgc aggtgtgtag
    58381 cccagcggcc agcgatgcgc gccaacatgg aactgcccgc cgcgaatggt tccctgcagg
    58441 cggttcactt cgcccataaa ttcgagtaca aaacgggtcg ctggttcgcg ccatacctga
    58501 ttcggcgcat cagcctgttc aatattcccc tgactcatca ccactacacg atcggctact
    58561 tcggtcgcct cttcctggtc gtgggtcaca aacacgctgg tgaatttcag ctcttcatgc
    58621 agttgacgca gccagcgacg cagctcttta cgcacctgcg catccagcgc gccaaacggt
    58681 tcatcaagca gcagaatttg cggttctaca gccagcgcgc gcgccagcgc cacacgctgt
    58741 ttctggccgc cggaaagctg cgccggataa cgatccgcca gatgcgcaag ctggaccatt
    58801 tccagcaatt ttgtcacttt cgctttgatg gctgcggcat tcgggcgctc gcgacgcggc
    58861 agcaccgtca ggccaaaagt gatattgtcg aataccgtca tatggcggaa cagcgcgtaa
    58921 tgctggaaca cgaaaccgac tttacgatcg cgcgcgtgca ggcgactcac gtcggtgccg
    58981 tggaagcgaa tatgcccgct ggtttgatgc tccagcccgg cgataatccg cagcagcgtg
    59041 gttttcccgg aaccggacgg ccccagcaac gcgaccatct gaccagaagg aatatccagt
    59101 gagatatcgt tcagcacctg ggtgcgacca aaagacttct tgatattggc aatctcaatg
    59161 ctcatgatgc tcctcctgct gcgcgcgttt ttcctgattc tccaggcgcc actgcaacat
    59221 actctttaaa aacagggtga taatcgccat cagcgtcaac agcgcggcgg cggtaaagga
    59281 gccgacggtg ttgtagtcct gctccaacaa ttcaatctgt aacggcagcg aaagtgtttc
    59341 gccgcgaatc gagccggaaa ccaccgacac agcaccaaac tcgccaatcg cgcgggcgtt
    59401 ggtcagcacc acgccgtaaa gcagcgccca gcgaatattc ggcaatgtga cgcgacggaa
    59461 catctgccag ccggacgcgc caagcaaaat cgccgcttcg tcttcctggc tgccctggct
    59521 taacatcacc ggcaccagct cgcgcaccac aaacggacac gtcacaaaga tggtgaccag
    59581 caccattccg ggccaggaga acataatttg cagattatgt tcatccagcc agccgccgag
    59641 cgggccgttg gagccgtaga acagcagata caccagaccg gcaaccaccg gcgatacggc
    59701 aaacggaatg tccagtagcg tcagcagtaa ctggcgtccc gggaagttaa agcgcgtcac
    59761 cagccaggcc agcagaatgc cgaacaccag gtttaccggt acggcaatca gcgcgatcat
    59821 caccgtcagc cagatggcgt gcagcatgtc cggatcggcc agattctgta aaaccggcat
    59881 cagccccttg ctgaatgcct gcacgaagat gtaaatcatc ggcaccagca ggatgaacgc
    59941 cgaaaccagc atcccgatgc caatcagaaa ccatttgccc cagttaatcg ggcgcgcgtc
    60001 ataacgcttc aattgggtaa cttccgccat taatgaccta ccacacgccg accaaagcga
    60061 ctttgcagag tgttaattga gaacagcagc agcagagatg ccgcgaggat caccgaagca
    60121 atcgcgctcg ctgccgggta atcaaactcc tgtaagcgca caaaaatcat cagcgacgtc
    60181 acttccgtct tccacgcgat atttccggcg ataaaaatca ccgcgccaaa ttcaccaaga
    60241 ctacgggtaa acgacagcgc cacgcccgcc accagcgccg gagaaagctc cggcagcacc
    60301 actttgcaga aactctgcca gcgcgttgca ccaagcgttt ccgccgcttc ttcatattcc
    60361 gggcctaact cttccagcac cggctgcacg gtacgcacca caaacggaat gctggtaaag
    60421 gccatcgcca ccgcaatacc aagccaggta taggtgactt tgatatcaaa cttcgccagc
    60481 cattcaccgt aaaaaccgtt tacggaaaag agcgaggcca gcgttaaacc ggcgactgcc
    60541 gttggcagcg caaagggtaa atccatcagc gcatcaagca gcgtgcggcc cggaaagcga
    60601 taacgtgtta aaatccacgc catcagcaga ccgaaaacgc cgttaaaaat cgatgccaca
    60661 aacgccgaca gcagcgtcac tttgtaggcc gcgaccacct gcgggttggt gatcacctcc
    60721 cagtactgcg cccagctcat ctcagaaagc tgcatcacca gcgcagagag cggcagcagc
    60781 aatatcaggc acacaaacag cagactggtg ccaaggctta aagtaaagcc cggcagcacg
    60841 cgtcttgaag aaactgcaaa cattacttac gccccgccgc taacagcttg tctaactcgc
    60901 cgccgctggt gaagtgggtt ttcatcactt ccggccatga gccaaatttg tcttccacgc
    60961 ggaacagttc ggtctgcggg aatttatctt tcagtttgtc catgacttcc gggttattca
    61021 cacggtagta atagtcggtg atgatggtct gcgcctgtgg gctgtagagc cagttcaggt
    61081 aggcttttgc ggctttttcc gtaccgttgg cctgcacatt tttatcgacc cacgccaccg
    61141 ggaattccgc cagaatattg gttttcggga tcaccacttc aaagccctgt gcttcatact
    61201 gtttacggat gttgttcact tccgattcaa agctaatcag cacatcgccc aagccacgct
    61261 cggcaaaagt ggtggttgcg ccacgaccgc cggtatcgaa cacttcaacg tttttcagga
    61321 actgggtcat aaactgttcg gttttggctt catcgccacc gtcagcttta tccgctgcgc
    61381 cccatgccgc cagataggta taacgcgcgt tacccgacgt tttcgggttc gggaaaatca
    61441 gcttcacgtc ggagcgcacc aggtcgttcc agtcgtggat attcttcggg ttgcccttac
    61501 gcaccaggaa gcccatggtg gagtagaacg gcgagctatt attcggcagg cgcgactgcc
    61561 agtcagccgg gatcagcttg cctttgtcgt gcaggatttg tacgtcggtc acctggttat
    61621 aagtgacaac gtctgctttt aagccctgca aaatcgccag cgcctgtttt gatgacccgg
    61681 catgagattg ttttatcgtc agtttgtcgc cgccgttatc ttttgcccat tgctgttcaa
    61741 acggcggatt cagggcggca aacagctcgc gggagacgtc ataagaactg ttcagcagtt
    61801 ccgttgcctg tacatggccc gccagcagca gagaagcgac cagcgcgagt gagttctttt
    61861 tcagtaagtt aacggccatt gcgcaccctt ataaatttaa tgactttcta atagccatca
    61921 tatttataac ggacgtgaaa ggagtaacgg ttttatatac cgtttggtga tttggaagtt
    61981 aaaaagggaa taagaacttg gttcaacagg cgtaattgtt gcaggcagtt tggacacgga
    62041 cagcgcggag aaaccggagc gtacacatag tacgtgagga tttcgagcac tgcccggggc
    62101 caaaatgaca aatgaaatag cctggtgaac ttagttcaag acgaaatcct ccccacacat
    62161 gcagggagga aacagaggtg aatcagatac cgacgctaac cgtctccggc agtgtgctgc
    62221 cgccatcaat cacattctgt gtaccggtta aatagctgga ttcatccgat gcgaggaagg
    62281 ccgccagttc gccgacttcc agcggatcgg cgaggcgaca cagcgggatt gcttttgcca
    62341 tttcagtcag caccgattct ggatcttccg ggttcgactg gcgggcaatg ctttccgcca
    62401 ttggcgtgcg gacgtatccc gggcagatgg cgttaacgcg aataccggat tgcgcgtact
    62461 ccaccgccag cgatttagtc aggccaacaa tcgccgcttt cgttaaggca tacgccgttt
    62521 cgccaggatc ggccaccata tcaccagtga ctgaagacat catcacaatg cgaccatctt
    62581 tgcgcgcaat catctccggc agcaccgcct tcgtgacgtt ccatacgcct ttaatattga
    62641 tatcaatatg gaaatcgcga tcttcatcgc tcatatcgag gaaactgccc agacgacaaa
    62701 cgcctgcgtt attcactagg atatcaatgc gcccttcttt ttccttcgcg cgtttgatag
    62761 ctgcggctac cgacgccggg tcacgcacat cggcgacaac cgccgtacag cgatgaccac
    62821 gaccacacag ttcgtccgct agcttttcga tctcagggga gatatccagc aagattaggt
    62881 tcgcgccatg acgtgcaaaa gttctggcaa ttccttcgcc aattccctgc aatgcgcccg
    62941 taatcagtgc tgtcttgccc gtgagtttac ccattttaat gctctccttg ttaatacaga
    63001 gcgttacact ccattccctc tgaaaataca ggataaacaa aatattgctg cggatcagct
    63061 cactaattca ggaaaatctc aatccagtcg ctgctttatg catcatttca ggcgttgtgt
    63121 tagctcactg ctgcgctgaa tcattttcaa tgacgccaca tcattgatgt tgcacaagac
    63181 caacaaacag taaatcggta acagaatttt gcgccgtgcg ggtagacatc gacgagctac
    63241 gccattcggt ttcaccggaa acggtatcga gcgtgaaatg cgccagccgc cgcagcggag
    63301 agtcagccag agaagtaatg gcaatcaccg ttgccccctg ctttcgtgcc gcttctgcgc
    63361 acaagacaat ctctttttta ctgccgctgt aagagatagc gatttgtaca tcgccttttt
    63421 tcaacgcctg ggaaacggtg gcttgtacgt gcgtgtcggc ttcgcaggcc acgcgatagc
    63481 caattttcat cagtttgaac gacagatcgc gccccaccag cgccgatccg cccaggcccg
    63541 ttatctggat aaacggtgct ttactgatga cctcaatgat tttttgcagc cgcgcgtagt
    63601 cgaacaacgc acaggtttgc tccagcgcca gctctttttc gcgattcagt ttgcgggcaa
    63661 tcacttccag cgaatcatca ctggtaatcg aactgtgcag gtgcagcgcc gtggcattcg
    63721 ttttctcccg gctggcactg tattcgccaa ttaacgccat acgcaattca gtaaatcctt
    63781 gcgcgccgag cttctgggca aatttcacaa tgctcgactg gctgataccg agctgtttcg
    63841 ccatctgccg tgaagagacg gatttcagct cgctcactct ggcccgcaga aagtcagcaa
    63901 ttttttgctc gttcccggta aattcacttt ccgcattgcg tattttcgtc aggtacaaca
    63961 tcttccactc tcctgggctg gcagcaaaaa ttccgcttaa gttcttatga cgctcttcac
    64021 actctgcgag tgtagactca atgattcctt taagtctgtc aattcgggat atatgattcc
    64081 atacatctgc aattatttga tcgtaaatag taaggtcacc accgatgcaa ctagaaaaga
    64141 tgattactga aggctcgaac accgcctcgg ctgaaattga ccgcgtatcg acgctggaaa
    64201 tgtgccggat tatcaacgat gaagataaaa ccgtaccgct tgccgttgat cgcgtcctgc
    64261 cggatatcgc cgcggcgatc gatgttatcc acgcccaggt cagcggcggc ggtcgtctga
    64321 tttatctcgg tgcgggaaca tccggtcgtc tggggattct ggatgccagc gaatgtccgc
    64381 ccacctacgg cgtgaaaccg ggtctggtgg ttggtttgat tgctggaggc gaatatgcca
    64441 ttcagcacgc ggtggaaggc gctgaagata gccgggaagg tggtgttaac gatctgaaaa
    64501 atattaattt aacggcacag gatgtggtgg ttggcattgc tgccagcggt cgcacgccgt
    64561 atgtgattgc cggactggaa tacgcacgcc agctcggctg ccgcacagtg ggaatttcct
    64621 gtaatccggg gagcgccgtt tcaaccaccg ctgagtttgc cattacaccg attgtaggtg
    64681 ccgaagttgt taccggttct tcgcggatga aagcaggcac agcgcagaaa ctggtgctca
    64741 atatgctttc caccgggctg atgattaaat ccggcaaagt gttcggcaac ctgatggtcg
    64801 atgtggtcgc caccaacgaa aaactgcatg tgcgccaggt caatatcgtt aaaaacgcca
    64861 ccggatgtaa tgccgaacaa gcggaagcgg cgttaattgc ctgcgagcgc aactgtaaaa
    64921 cggccattgt gatggtgctg aaaaatctcg atgccgcaga agctaaaaaa cgcctggatc
    64981 aacacggcgg ctttattcgt caggttttag acaaggaata acccatggcc aaagagatca
    65041 gcagtgaact tctgaacacc attcttaccc gtgtcggcgg accgggaaat atcgccagtt
    65101 gtggtaactg tatgacgcgc ctgcgtctgg gtgtacatga cagttcactg gttgatccca
    65161 atatcaaaac gctggaaggc gtgaagggcg tcattttgac cagcgaccag gtgcaggtcg
    65221 tttttgggcc tggtaaagcg catcgtgccg caaaagcgat gagcgagctg ctgggcgagg
    65281 caccggtaca ggatgccgca gaaatcgccg cccagaacaa acgtcagtta aaagccaaac
    65341 aaacctccgg cgtgcaacaa tttctcgcca aattcgccac catcttcacg ccgctgatcc
    65401 ccggttttat tgccgccggt ctgctgctgg ggatagcgac gttaattgcc acggtgatgc
    65461 acgttccggc agatgctcag ggcacgctac ccgacgcgct gaattttatg aaggtgttca
    65521 gcaaaggttt gttcaccttc ctagtgattc tggtgggcta taacgccgcc caggcatttg
    65581 gcggcacggg cgtcaatggc gcaattattg ccgcactctt tttgctcggt tataaccccg
    65641 ctgcaaccac cggttactac gccggttttc acgatttctt tggtctgccc atcgatccgc
    65701 gcggcaatat tatcggcgtg ctgattgccg cgagggcctg cgcccgcatt gaaggcatgg
    65761 tgcgccgctt tatgccggac gatctcgaca tgctgctgac ctcgttaatc accctgctga
    65821 tcaccgctac gcttgcgtac ctgatcatta tgccgctggg tggctggtta ttcgaaggta
    65881 tgtcatggct gtttatgcac ctgaacagta atccgttcgg ttgtgcggtt ttagccgggc
    65941 tgttcctgat cgccgtggta tttggcgtgc atcagggctt tattcctgtt tacctcgcgt
    66001 taatggacag ccagggattc aacagcttat ttcccatcct ttcaatggca ggcgcgggcc
    66061 aggtgggcgc ggcgctggca ctctactggc gggcgcaacc gcacagtgcg ctacgcagtc
    66121 aggtacgcgg ggcgattatt cccggcctgc tgggcgttgg tgaaccgctg atttacggtg
    66181 tcaccctgcc ccgcatgaag ccgtttgtta ccgcctgttt aggcggcgcg gcgggcggtt
    66241 tgtttatcgg attgatagcc tggtggggtc tgccgatggg cttaaacagc gcctttgggc
    66301 cgtctggtct ggtggcgctg ccgctgatga cttccgcaca aggcatcctt ccggcaatgg
    66361 cggtttatgc tggcgggatt ctggtggcat gggtttgcgg gtttattttc accacgctct
    66421 ttggctgccg taacgtcaat ctggactgat attatgaaac ggacaatgct ctatctttct
    66481 ctgctggctg ttagttgtag cgtcagcgcc gcaaaatacc ctgttctgac agaaggtagt
    66541 tgatcaagta tatgcggcgt taaagcagta ataaaaaggc cagtcagtaa tgttactgcc
    66601 tggcctttaa tcaatgaatc agaaacgctt acagcaccat caacttgtcc agcgacggtg
    66661 cgaaataata gccgccggtt accggtttgg tgaaacgcaa catcgcatca cgtttgccat
    66721 cggtatcgcc aaacatgctc agcagttgct gctcaatgtt atgcagacgc gcgcagtagg
    66781 cgcagaagta cagaccgtga gtgccactgg cggtgccgta cggcaggctc tggcgaacaa
    66841 tcttcagccc tttgccatct tctttcagat caacgcgggt gaggtgagag gtttccggac
    66901 gttcgtcgcc gtcaatctct tcgttggcct ctttggtgcg cccgatcatc atctcctgat
    66961 cgtgaacgct catccggttg agctgcttca ggttgtgctc ccaacgctgc acaaacacat
    67021 agctgccgcc ggcatccacg ccgtctttga taaccgccac ttcgcggcgc gtctcttcac
    67081 ccgccgggtt ttctgtaccg tcaacaaagc cgctcaggtc acgctcttcc acccaacgga
    67141 agccgtggat ctcttctttc acttcaatgc agtcaccaaa ggcttccatc gccgcctgag
    67201 cgacagagaa gtttacgtcg tgacgcagag aaagaatgtg gatcaacaca tcgaactggg
    67261 tcgtcggcgc aaggccttta ccgtagcccg gaaaatcttt cagctcttct gccccaacgc
    67321 cgccgctcag agcgcgccag gtgttgttac caaaggcaac caccgcacca agatgcgcgt
    67381 ccgggaattt cgcttcaaaa gttgccagtt tgtcggcaaa tgttttactg gccgcacgca
    67441 gggcgtcaac ttcccctttc acgttggctt cgatccaaat cgccgcgcgg caatgttctg
    67501 gcaaaatacc actctgaacc tgagacattg ttcctcctga aaataagaat gccacgcaat
    67561 ccgtggcatt gttgagcgct attttacgtg ttttttcgcg acggcggttt gttcagacgc
    67621 aaattaacgc cgccagataa ttttactgac tttccagttt ttcagggtat cgtccgaagg
    67681 cattaaccct tcaggaccac gccattcccc gctgaactgg tagctaatat gttgactgcc
    67741 ttcggcttta cattcgacgg cacgattatc atcaccatca gctttttggc aattgccaaa
    67801 tgctttgctg taaaggtcgc taaacggtgt accgatttta acaccggtgt cagcaggaat
    67861 atcgctatcc agcacgtcaa tgcgactgat cgtgccttga tcgccgttaa tcaccatcgc
    67921 cacgttgtcg cctttcatca cttcaaaaaa gcgcaccacg ttgccgttcg cggttttcat
    67981 tccgctgcgc aggcgataat cgccatcaag cgcatcagca atggcttgtt cttgcagtgg
    68041 tgtggacgcc gttaattcac ccacgccctg ctcgctcact ttggtggacg atccaaacca
    68101 gttccacggg ttagcggcag accagttaac tgacgacatc gtggaacagc cggtcagcat
    68161 caacggcata gcgcataaca ttaaacgcag cgatttcatg tcacttcctt tggttattca
    68221 ataacgttgc ttggagtgca aattcaccaa aaagtgccgt tattcttttt cttcattaaa
    68281 acaagcgcgt aaacgtcgat tggtcagcag ccagattaac gccacaatat ccgccactac
    68341 cagcgccaga ccgataccgc taacggattc accgttcagc cacagccacg gttgccagca
    68401 aagtaaaacc acctgcgcca acagcaacag aaaatagagc acatgccagg tgcgaggaaa
    68461 cgaagcccgc cgaccgctca acaaaaacgc cagcaccgcc ggaataccgg gaatcagccc
    68521 cagccagaaa ttatcgtgat cgggataaaa cagatttagc agcgcagtac cctgctcgcg
    68581 cgacgcaccg gcaatgacaa acagcaccca ggttcgcgcc tgaagcaata gcacaagcca
    68641 gaagagcaag ggtaaacgca ggcgtccgtg cgcatcataa tggacaggat ggaactcagt
    68701 actcttcatc ttcaatcaaa cgcttaccca gactcagcac gtcggcgtgt tcatatccca
    68761 ggcgttcata cattccgagc accatgtcgt tatcttccgg cacgttgatc tgaattttcg
    68821 ggcagccgcg agcaatcagc tttttctcca gccggttaag caacgcattg gcaattccac
    68881 gcccacgaaa ctctggatgc acgccaagat aataagcaga cccgcgatgc ccgtcataac
    68941 cgcccatcac cgttccgacc acttcaccgt ttacctcagc gaccaaaaac aaactgacgt
    69001 catggttcat cttacgctcg atgtccattt ccggatcgtt ccacggacgc agcaagtcgc
    69061 aacgctccca aagggtgatg acctcttcga aatcttcctg gcgaaatacg cgtatctcca
    69121 tggtattcgt tacctttttg cgggttaaaa ggctgattat ggcgtgaacg gtcgaattag
    69181 ccaatatctg acgaaaatcg gttgaaaaag tggcataatg gggagttgtc aactattgaa
    69241 atgaaaagta aaacaattct caacagcaaa ccgtcgtaac ggattacgcg atacgatata
    69301 acatctggaa ctttattatt acaactcagg ccgtatgagc acttttaaac cactaaaaac
    69361 actcacttcg cgccgccagg tgctgaaagc cggtttagct gccctgacgt tgtcaggaat
    69421 gtcgcaagcc attgccaaag acgaaccttt aaaaaccagc aacggacaca gcaagccgaa
    69481 agcaaaaaaa tctggcggca aacgtgtcgt tgttctcgat ccaggccacg gcggaattga
    69541 taccggagcg atcggacgca acggttcgaa agaaaaacat gtggtgctgg cgattgctaa
    69601 aaacgtccgt tccattttgc gtaatcatgg gattgatgcg cgtttaacgc gttctggcga
    69661 tacgtttatc ccactttacg atcgcgttga aatcgcccat aaacatggcg cagatctgtt
    69721 tatgtcaatt catgccgatg gctttaccaa cccgaaagct gccggtgctt cggtatttgc
    69781 cctctctaac cgtggggcaa gtagcgcaat ggcgaaatac ctgtctgaac gcgaaaaccg
    69841 cgccgatgaa gttgccggta aaaaggcgac tgacaaggat cacctattgc agcaagtact
    69901 gtttgatctg gtgcaaaccg atactattaa aaacagcctg acgcttggct cgcatattct
    69961 gaaaaagatt aagccggtgc ataaactgca cagccgcaac accgaacaag ccgcgtttgt
    70021 ggtgttgaaa tcgccgtcgg ttccttcggt gctggtggaa acctcgttta tcaccaaccc
    70081 ggaagaagag cggctgttag gcacggcggc gtttcgtcag aaaatcgcca cagcgattgc
    70141 tgaaggcgtg atcagttatt tccactggtt cgacaaccag aaagcacatt cgaaaaagcg
    70201 ataagttatg aaacccgacg cacaccaggt taaacagttt ctgctcaacc ttcaggatac
    70261 gatttgtcag cagctgaccg ccgtcgatgg cgcagaattt gtcgaagata gttggcagcg
    70321 cgaagctggc ggcggcgggc gcagtcgggt gttgcgtaat ggtggtgttt tcgaacaggc
    70381 gggcgtcaac ttttctcacg tccacggtga ggcgatgcct gcttccgcca ccgctcatcg
    70441 cccggaactt gccgggcgca gtttcgaggc gatgggcgtt tcactggtag tgcatccgca
    70501 taacccgtat gttcccacca gccacgcgaa tgtgcgtttt tttattgccg aaaaaccggg
    70561 tgcagagccc gtttggtggt ttggcggcgg cttcgattta acccctttct atggttttga
    70621 agaagacgcc attcactggc accgcaccgc ccgtgacctg tgccagccat ttggtgaaga
    70681 cgtttatccc cgttacaaaa agtggtgcga cgattacttc tacctcaaac atcgcaacga
    70741 acagcgcggc attggcgggc tgttctttga tgatctgaac acgccagatt tcgaccactg
    70801 ttttgccttt atgcaggcgg taggcaaagg ctacaccgac gcttatttac caattgtaga
    70861 gcgacgtaaa gcgatggcct acggcgagcg cgagcgcaat tttcagctct accgtcgcgg
    70921 tcgttatgtc gagttcaatc tggtctggga tcgcggcacg ctgtttggcc tgcaaactgg
    70981 cgggcgcacc gagtcgatcc tgatgtcaat gccgccactg gtacgctggg agtatgatta
    71041 tcagccaaaa gatggcagcc cagaagcggc gttaagtgag tttattaagg tcagggattg
    71101 ggtgtaactc cctccccccc attcccgcat ccgctgatgc aacgtcaacg acggcttctc
    71161 ggcaaacagc tgctggtaat ccgtggcaaa ttgccccaga tgccagaatc cccactgcat
    71221 ggcggcgtct tttaccgttg tgctttgcga ccacgggctt atcagttcgc ggcgtacggc
    71281 gttcaggcga atgcgtttca gccacgcgtt tgggccaatg cctaaaatag cgtgaaacgc
    71341 gttttgtagc gtgcggcgac tgacatgcag ttgattacac aaatccagca ccgtcaccgg
    71401 ttcggacatg ttttccagca catattcacg ggcgcgggaa agcaaccgac ggtaactctg
    71461 atgactgatg ctttccgccg tcaccattgg ttgcgcttct tccagcatcg cccccatcgc
    71521 cattagcaaa ttatccccca gcacttttcg cactgcaggc tgatggagat tttccgggtt
    71581 ctcgcaaaac gtcgccagcg cctgttggac aaagccccac agcgcggctt tatgctgctc
    71641 tttcacttcc agcgccgact ggctacgcag catatgtaat acccgatccg gattgtgcaa
    71701 aaagttagcc tgacaggtga tgacatcttc agacagcact acgcccagga tcgtataatc
    71761 gtccggcgta cttaattcaa actcggttcc accggggcgc gttgctattt ctgcgcttcc
    71821 cagacattgc gaaccgataa atccctgctc accgcgcgtc gccggaatgc caaaccagaa
    71881 cgagttcggc cagaccaggc acgactgacg cagcgccaga ccggtgtatt cacgaaaaac
    71941 ctgaatatca tcgagtagaa tttccgtaaa ttcaccatga aacttgcccg gatgcagctg
    72001 atcgtaaatc tgctgccagg cggtaatcgt caaagcatgt tcatagacat ccgttgtctg
    72061 tcgttgatga acattatcca cttcgacctt cggcgtgagc ttcaggtttt cgggtaaggg
    72121 ttcatgataa agatggtgca aattggctgt acgggtcttt ttcatgatgt taatgccggg
    72181 tgttgtagga cacccggcac ctccgacagg ttaatggggc ttgagacgat aacgactact
    72241 gcgtttacgt tacgtcccgg cagaaaagag ctgttcgagc gcatttctgg ctttttccag
    72301 cggccagcca aagtgggcgg ccacttctcc tgccgtcatt ccctgacata ctgatgttaa
    72361 cagcgccaac aactcgtcag cagattccgc gacgataact ggcgcgtcgg gttcctttac
    72421 gggttgcttc ggctggcggt taaagccagt aaccagccac tgggtgtcat cgtctggccg
    72481 accgatctcc ttacggctga tgacacgccc ggtacgcatc gcggcagcac aacctgcgtc
    72541 cagcgccgca cgacacgccg ccagatcgcc ttccaccacc agcgttaagc gaccagggtc
    72601 aagcacttcg tgactgagca gacgaacgtt agctgctttg agcatggcat ccgctgcatc
    72661 tatcgcggcg accatgccgt ccacttccag caatcccagt gcattgatca tcggcaacct
    72721 ccgttacgca cgctggattg gattacgcgc gatttccagc actgcatcgg taaaggcgtt
    72781 acaggctgct ttacacgcgg cctggctacc ggttaaaaat gctgccgagt agttggtttc
    72841 agacggtggc gggacgtagg tcaccagctg tacgtcggca gatttcaacg cggcatcaat
    72901 gccgtaggtc gcttccagcg gcggtgccac cagatacgcc atcggatcgc caagcgtgat
    72961 cccggcggtt gatgaaagat aagaacctgt acgcgaaact acatgcgcca ggaaggcggt
    73021 gttttctgcg tcattggccc actggaaagc tgcgccattt tcaatatgtg caaccatcgc
    73081 atccagaccg gcacgcactt ccgccgggtt tggcccacca agcataatca gcacttcacc
    73141 ggcagtcggt gacgggccgt gtgccgctcc cgcatacagc gagcgaccat ataccacttc
    73201 gaccatcgct tgtttggtcg cttcgtcagc cgcaatgtag gttacgtcgt cagaatcggc
    73261 agaaatcagc ccgaggctac gaatatgcgg cggcaatttt agttcacgcg caaattcagc
    73321 gttaacggag gcaatcaccc gcatggcggt taccgacggt cgaatcaaat ctaaagctgg
    73381 catgatgcct ccttatcggg tcatgttgat gccggatgct ttctgctcca gcatacgttt
    73441 cgccaaatcc acaatgacgg ctgcggcttc aaccggcggc gtgccgccct ggtgaatgtt
    73501 agagatacag gtgcgatcgg cctcaacggt ggtcgccata cgcggcgagt aaacggcgta
    73561 gcaggagagg ctttctgact gccctaaccc cggacgttcc cccaccagca ggatcaccac
    73621 tttcgcgccg aggatctcac cgatctgatc ttcaatcttc acgcgaccat aacgaacaaa
    73681 gaatggcgtg ccgactttca gcccggcctg tttcaggccc gccatcagcg gcggcaggat
    73741 ctcttcgtag ttgacggtga tggcatcagt tgacaggcca tcagaaataa cgacctgtac
    73801 gtccggatta gcaacacact gtgctttcag cgcttcaaca gcttctgcac acaggcggcg
    73861 gcccatatcc gggcgagtca ggtagagatt tttgtcgcta atttcagagc gtacttccag
    73921 caagccctgc gctttcaccc actcttccgg cacttctttc agtacggtat ctttcgaacg
    73981 agagtgatcg gccaagaaac gcagcagcgc ctgagtacgt ggacgcggac cggcacgacc
    74041 ggtacaaacg cgggccacgg tgctgcggcg cagttctgtt aatacgtctg cgcgatgcgg
    74101 gttttcaaca ccaatccacg cttttgcttc agcggaacct aaatccagcg cacagctttc
    74161 cgaggtcacc ggtgccgcac agttggtggt ggcgcacttt gcttctgacg gcgcgggggc
    74221 cgcttgtccc attgacgcca tcacgctgcg tacaatttct tcaatctgtt tttgatccat
    74281 gatgtgttat ctccgcgtca tcagaagaac agtgacggat cgcccgcccg tttggtcagg
    74341 cgaccgtttg ccataatgcc catgctttcc agccagcgtt caaactctgg tgacgggcgc
    74401 aggttgagta actgacgcac agtggcggta tcgtggaatg cggtggtctg gtagttgagc
    74461 atgatgtcat cacccagcgg catccccatg atgtagttgc agcctgcggt ggcgagcagg
    74521 atcatcaggt tttcgttgag gttctggtca gagtcagcgt ggttggtgta gcagccgtca
    74581 cagcccatag agatgccgct cagcttgccc ataaagtgat cttctaagcc agcacggata
    74641 atctggcggt cgttgtagag atactccggc ccaataaagc caaccacggt gttgacgata
    74701 aacggatcgt aatgacgcgc cagcccatag ttacgtgctt ccatcgtcac ctggtcagcg
    74761 ccgaagttag cgccagcgga tagcgcagag ccttgtccgg tttcgaagta gaggcagttt
    74821 tccccggcga tacgattgaa ctctgcgccc actgcgcgcg cttcgtcgag catcgccagt
    74881 tccacgccaa actctttcag ccctttttcg ctgccacaga tactctggaa aatcagtccg
    74941 ccaggagcgc cgcgacgaat cgcttcgatc tgggtggtga cgtgcgccag tacgcagccc
    75001 tgagttggga tgttgaattt gtcgatcacg ccataaatgg tatccagcac gcggcttaag
    75061 ttttccacgt cgtcagttac cgggttaacg ccgatcaccg catcgcccac cccgaaggaa
    75121 agcccttcat agatttgcgc agcgatactt tgcacgtcgt cacgggtatc gttcggctgc
    75181 aaacgggcgc taaaggtgcc cggaataccg atggtggtat tggccttttt gattaccggc
    75241 attttcttcg cgccgtagat caggtccgcg ttggagcaaa tcttcgctat cgccgcgacc
    75301 acttccgagg tcagcccttt gcgggtaaag gcaatgtcgt ccacgctggt ttcatcgctc
    75361 agcacatact cacgcagttc gctgatgctc cagtttttaa tctggttgta ggccgtttcg
    75421 ttaacgtcgt cctgaatcag ccgcgtcacg cagtcatctt cataggcaat caccggatta
    75481 ttgcggatgt ccgctacggt catttccgac aacacctgct ttgccgccac gcgctcctgt
    75541 gagcttgccg ctgcaacgcc cgccagcaca tcccccgaac gcagttcgtt ggctttagcc
    75601 agcacctctt ttacatcctt aaactgatat acattgccga acaatgtggt ctttagtttc
    75661 ataagtcgtt ccctcaggaa gaagtgatga gtaacatttc tcacagcctg caaacaattc
    75721 tatttcatcg ttagcccttg tcatgtaagg tgatttcctt aacacgcccg ttacttatcc
    75781 tgccgaacgc aaccgaactc gccggataca tgagcggggt tatcgagtcg ttagatcgag
    75841 gtatccggtt ttgagcaaaa caccactcac agcaaaggcc atagacgccg cacaaccaaa
    75901 ggacaagcaa tacaaactca ccgcttcact gacgcccggc ctgtttttgc tggtgcatcc
    75961 caacggcagc aaatactggc gattccggta ctggatagac aagaaagagc ggttacaggc
    76021 tattggggta tatccgctga ttagcctcaa ggaagcccgc aaacgcgcca cagagagccg
    76081 tttactgata gcccagggaa ttgacccaat ggatttggca cgcaaggaga aagccattga
    76141 tgcactcaac atggcggcag gttttaagac ggtagcggag gactggctgg ctaccagggt
    76201 aggcggctgg agtgagagct atacaaagca ggtcaaatca gcactggaga aagacgttta
    76261 tccggtactt ggcaagcgtt caatcgttga tataaccgcc cgtgacgtgc tggcattact
    76321 ccagaagaaa gaacgtaccg caccggagca ggcccgcaag ctacgccagc gcatcggtga
    76381 gatcttcaag tttgccgtta tcaccgaact ggtaacgagg aatccggttg cagatcttga
    76441 tacggcactg aaggcccgcc gacctggtca caacgcgtgg ataccgatta gtgaaattcc
    76501 ggccttctac aaagcccttg agagggccgg gagcgtccag attcaggcgg caatacgttt
    76561 gcttatatta acggcattaa ggaccgcaga actgcgtttg tgccgttggg aatggataaa
    76621 cctggaggac gcaacaatca cattgcccgc tgaagtcatg aaggcccgcc gcccgcatgt
    76681 ggtcccgcta tcacggcagg cggtcgagct attgcaggac cagttcacac gcagcggata
    76741 cagtgttttc gtgttcccgg gccgattcat ggataagcca ttatcagcca gcgcgatact
    76801 caaggcgctt gagcgtatcg gatacaagtc aatagccaca ggccacggct ggcgcacaac
    76861 gttcagcacc gcacttaacg aaagcggcag gtataaccct gattggattg agatccaact
    76921 agcccacgtc ccgaagggaa ttaggggagt ttacaaccaa gcggcctatc tcaaaatgcg
    76981 ccgtcaaatg cttcaggact acgccgacgc gattgattcg atactggctg atggtatcaa
    77041 tccgcttgaa ctgtaatgaa tgattaatca cgttttccga cgactccacc ggagtaggcc
    77101 ggataaacac tggacacctt caaaataact attcactttt tccggattcg tccggctttt
    77161 tttgtctttg ccgatgggat caattattcc aattattcca attattccag tatcgttgga
    77221 ataaatgaat aaatatcaat gacttaaagc cgtgtttgca acagggtgga tcggggatcg
    77281 aaaataggat caacacaaaa acaccctgga ggctacagca cactaggggg acacaaaaac
    77341 aacacaggaa aaaaatacca tgtaccaatc agcaaccgcc tacgtggcga aaccaggcga
    77401 aacactggtc aaacacaaaa ttgcaacact ccaagcactt caaacactcc aagcccttcc
    77461 ggaacaggtt caggccgcct ttcgtgatgg catgaacatc atccgcttcg atgggtatcg
    77521 ctgggaatac aagccagcaa ctggattcgt gcgcatagat caaatcttgt cgccagccaa
    77581 tgaagagcca gcgctagcca atgcagaacc agcgcaggag caggagcagg agcaggagca
    77641 ggaacaggaa caggaacagg aacaggaaca ggaacaggaa caggaacagg aacaggaaca
    77701 gaataaagcg gcggctattg ccgagttcaa ggagctgcaa agacaacaaa tcacagcgca
    77761 gcaagaagaa tgccgccacc cgctggcaat gcccaagcca gtagcgaaag ccaacacaga
    77821 caagccgact tcatggttta acgaagaagc ggaagaagat ttgcgttttg atagtaaaga
    77881 ggagcagttg cctgaagagc gggaacaggt ggaaacggaa gaccagcaaa acgattatcg
    77941 caaccttcca gccgtcaata acgctatggc ttacctcttc aaaagcgggc tgtttcacaa
    78001 gctgaaaggc gatgagttaa agctatatac gtggatggta tccaagtgca ggtatcgcgg
    78061 taacgacaaa tatgagattt ggtattcccg caccaaaatg gcaaaagcgt taggtaagag
    78121 cgttgacacg atagatcgcc acctggaagc gttgagaaag ctgggactta tccaggaaat
    78181 gcgccaggca ctgaggggca aatgtggaaa aacctacaga ctgcgaatcc cgtcaaaagg
    78241 cacagcagaa gaggcctcaa tgacggcaca gcagcacgaa agagaagcaa aagtcagcta
    78301 cctggtacac taggcagcgg gtcagcaaag gggggcaaat gccccttttt tcatgtcaga
    78361 taatcagatc attacctcaa taaactaata atcacatcgc ggttacaacc agtttgatag
    78421 tgatagctca aacccagacg tggcgctggc tgcaagcaaa ataagccgca aaaatgcggt
    78481 cctatttgcc agaacctttt attattcaat tggttagtgc aagaaaaagc gtgattactg
    78541 ttcaagaagc atccagaaag gtcatgattt tgccatgatt ttgacgctga aaaaaaatag
    78601 gtgggcattt ttgcccactc actagccgca aatttgccca ctcactagcc gcatttttgc
    78661 ggcccaaagt aataaataca gagaaataat aaatacagat cagcgccaag atttttcgtc
    78721 ttttcggcgg aatcatgaga ctagtgctta cgtcctgata acctccagag cagaccttaa
    78781 cttctgtgca gcaggactac gctcattgcc gctcggctag gtcaaatcct cacgataaag
    78841 ctgttcggat tgtgaccata atgttaaagg actctttaaa tcattccttg acgaattaac
    78901 tagctggctt aagcggaata gcatgcagga taaaaacaaa accagcttag cagtggggag
    78961 agcttcgctt cgctcgtctc ctggggccaa tgctcaatga aacgacgtaa ggcacggagc
    79021 gctcaccata ggtagacaca taaacatctg agagcgcacc ttctatcgcg ctacgcgctc
    79081 aataaaaacc aacgacaccg ccacactgca agaccaatgg tttttatcgc gtatacagac
    79141 cagacaggaa acagcagcaa agcagcgtat agccaggtgc agacagcaca ctcacacgac
    79201 gcacagcagg cttaagtgat cactcaggaa actttatccc cgccgccgat caccgcgaac
    79261 tatcatgacc gcgccaaatt cgaaaggaat ggcccataga acgcgatgaa cacacataag
    79321 cgctaacttg cttacctgaa aacttaacgt cgccacagag cgattgagag cgcttaaaat
    79381 cgatgcgatt tcattaaatt tttctcactc gcctgaatgg tggcgatcag gactgatcgc
    79441 aacattgccc cgcctcgata ttgagcgcat aatagataca tacattaagc agaaaagaga
    79501 taagtttatt gtgaaaatca gggggttaca catcatgcgc atatcagaac aacaacggca
    79561 gatcctcgac tatctggcaa aaaacgaatc aatcaccgca cgacaagccg cagatctcgt
    79621 ttatggtggc aatgttaccc gcgtccaggt tgacgccgcc cgccgctcac tcatgacgat
    79681 ggttagtaat gggctaatca ggaaacaagg ccgcgctttc gtagctgaaa gacatatcaa
    79741 tcacgcttat agccaggacg ttgaagaatt gcgccgccag atcctttggg tattcagcaa
    79801 acagggtcag gagtatttcc tttctgaaca cccaaatttc gccgccaaag agttaacagt
    79861 aagcggattg tcgtgcgcac tggattatct tgtcggtgaa ggttacgtta cttacaagca
    79921 gctatggcac gccctgaata cgcttgtaga agatggttta ctcattgcca cgcgtgagaa
    79981 catcgaatac tggataatgc cgcgttcagg tcgccgcaat catgcacggt atatttcagg
    80041 caacagatac agaacgccgg actttaatcc gacacaggaa gatcgggacc gtgccgaaat
    80101 gatgtgcagg gaataggaga aaagaattat ggataagtta ttttcgatgc agtgaacaca
    80161 agcgggcgca atgcccgttt ttattgcctt attccacgcc tgacgaatca atttgatcac
    80221 acctgagcaa atgcatagcc agaagttaag gccgttatat tgcgattggg agcgttttaa
    80281 acggtatcaa tttaattaat tctgatgtgg tgatttgcag gcaaaaaaaa cccgccggag
    80341 aagcgggaaa tagagacata tcgttgtagt tattaacaga tcactttgag gtcgagggcg
    80401 gctaaccctc gctaatatta tcccgccatt accttgcggg tgcaaataat cttttattgc
    80461 tttgatgatt taataaatag cactttttgc taacaaaaac cgctgtcaat atatggccaa
    80521 aaatgtcaac agaaaaaata aggtttataa tattaagtat cacatgggta atgggttcag
    80581 ggaatcggaa tccaccgcct cacggaaatt caaaatttgc ttcttctttc ctccgtgggg
    80641 cggcttcttt ttcgtatcga ggtatttatg aaagcaataa cattagaaca gttcgcagca
    80701 atggcgaatg tatcgaccag aacgctgagg cgctggctaa aaaaaccatc aaccggagtc
    80761 cctttgccat ttaccccacg cggtactcgc caattattct gggagcacga atgcaaagaa
    80821 tggttgtaca gaacagaacg ataataatca cgcatcacat tttgaggtaa acatcatgac
    80881 caatatcgaa agattagaac gctgtgccga tattatgcgg cgcagatgga tttatgaccc
    80941 tgaagcgggt ttattgttta gccgtgaaac aaaagacgtt atcaaaggca gcatgaccaa
    81001 taaagggcat ttggttgtaa cggtacatga aggtaaattc aaagtgacac tcacctatca
    81061 gaaagcctgc tatgtataca catacggcgc ttatgacgag acattatatg aagtgcacca
    81121 cgttaatttg gacaaacagg ataaccgcat caagaatatt cgcctgatgc cgaaagaaaa
    81181 gcaccgccgg atacataaaa acgtccgcaa acttattcgg ctgggccaca cacaatggct
    81241 tcaaccgcaa ttaattgaac aatcactata aacacagtag taaataccat gcattcatta
    81301 gacagttatt ttcagcgcac gacagcccct aaatcagccg cccaggaacg ccgtgaggaa
    81361 ttccatgaga aagtgatgcg ttctgctgat tatattgcgg acaagttcgt ggaaacggtt
    81421 cgtccacttg ttgatgaagt agctgataaa cttcagtctg aaatgccgga agatatggaa
    81481 ggcactgcaa agcggcgact catttgctaa ttaagtcgtc gttttggcgt gtcaataagc
    81541 gcttttaaat aatcgaaaaa aggggggaaa gataatggac gcatttgagg tactggcaaa
    81601 taaaggcgct gaactggtag cacagcgtga taaaaccgcc aacgaagggg aacgcgcact
    81661 gctaaataaa cagattaaag cgatccgtat ggcccagttt aaattaatca gtaatgaagt
    81721 tatcgaaaca attaaaccgc agatcccaca aatcgttgct gacgctatta aagccgccgg
    81781 actggagaaa cgcattgccg ggctaaaaac gaacgcagag aaaatggaga tgcacaaaca
    81841 gagcggtgct ccgaatcccg atgagtactt ctcaccggat gttgagttta tggcgcaggt
    81901 tgaggaacgt ctgggtagct gtctcaccga ggaacagcgt cgctattttg atggagtgga
    81961 tagttcggca ggtattgacc tgaactcgta tttcggtcgt gaaattgagc attttgatgc
    82021 cgccccgtct atgccggagc cagaaccaga aatggccacc gatcctgaag ctgagcaacg
    82081 gaaacgcgta ttcaatgcga tagggattcg gtactgatcg gataaaagaa agccgggaat
    82141 taccccggct gacttaacaa tgtgaccaat gaacaaatcg gacaagacaa acaggataag
    82201 cagtctcgaa acaatgttcg ggaactatat tagcacaagt ccggagaaaa tcatcatgtt
    82261 ggcactacgc atcatgcagg gcatcgctaa gaccctcgca gaacatgtct tagacctgaa
    82321 acattcgcca ctgagcaaac aggccatgaa gcgctaaacg cttcgtctgt gggctgaata
    82381 ttcgcttgga actatcaata aaatcatcga catgaaatcg gggccgagca accagagcgc
    82441 ggaggaaatg gagttcatca agcgtttaat tctcattcgc agagacattc atagccagtt
    82501 acacagcgtg ggaatcgata ttaatgatgg gacgggcgat taattttcgg acaagtacta
    82561 tgcgcgcgcg aggagctgaa catgaagcaa gatattgcag acaggctgga gatactcgaa
    82621 ggccagcggg cagaagcgaa acaactacgt aagcaggcca gaagagcgca caggaacaac
    82681 gaagcagagt tactaaccaa atacatcagt ttcaccaatt actgcatcta cgagtgttac
    82741 aaggaagatg cagaggactg gctggattca ttgccagaac agtactgagc agaataacac
    82801 cacgtcatgt tgttctgagg tgagcatatc acctctgttc aggtggccaa atttagtgtg
    82861 ccacttcagc atcaccgttg aaatcacaat aacgattttt ttgtaaatat gctctcacat
    82921 atgtaggaat agaccaggtt ggcattgaaa cacatctacc atgaccataa taaagtaatt
    82981 ttttgatatg tttatggttt tatattaatt aaaatatatc taaaattgat agttttaaaa
    83041 ttgcaaaatc cagattgtgt ttgtaatgtt taatctgatt gtattgagtg gcgtttatta
    83101 tgaaaaataa atcaggtaat tcttgttata gagaagcagt cgatattcat agctcacttg
    83161 agctttcatt tatattgcat aaatatcatt tgcgtgtaga tgagaagaaa tctcgaaatg
    83221 tattcagcgc catacgtgat gagctcatcc ttgatgggga ttcacgacaa aacttagcta
    83281 ctttctgtca aacatggctt gatgatgaaa tccgtgagtt gcttaattta gctattgata
    83341 aaaacatgat ttataaagat gaatatccac aaatggcgga acctgaaggt cgctgtgtgc
    83401 gtatgctttc cgattcgtgg agtttccccg attcgcgaca caccttaggt tgctcaacca
    83461 ttggttcatc tgaagctgct atgctcggcc gcttggcgtt aaaatggcag tggtgtaaaa
    83521 aacgcgaagt acaaggtaaa tcaaccgaac ctgatttgcg atcctgtgca aatatgctgg
    83581 cataaattct gccgttactg tgatgtgaaa ttgtgtgaaa ttccacttga tgatcatcac
    83641 ctgataatga atgctaaaga ggttttgaag catgtcgatg aaaacactat tggtgtcgtt
    83701 acgactttag gtattactta tttttgtaaa gcagattgcg ttagtgtttt taagataagt
    83761 gttaaagtta atattagttt ttatatttac atcgtaatta tatgtttcta ttttaaaaat
    83821 attcgagagt ataaaaatgt ctaatgctaa tatcgcaccc aaaagtaaga aaatgacagt
    83881 ggggacactc gctatcatga atataactgc tgtagtaagt ctacgattta taccttctga
    83941 agcagaatac ggcttaggtg ccatttttta ctataccttt gctgcaatta tgtttttagt
    84001 acctgttgcc tttgttgcgg ctgagctagc tacaacatat ccacaaaaag ggggggcatt
    84061 tcgatggata ggagaagcat ttggggctcg ctggggtttt gtaggaatgc ttatgacctg
    84121 gctacaagtc atcccctact ttccaacagt actgacattt ggtgcagtct ctgttgcatt
    84181 tattgatgtt gatatggggg tggcagaatc aattgccagt aataaatggt atattttatt
    84241 tttcgtacta tttgtttact ggggagctat ttgtgttgca ttacgtggcg ttggtatttt
    84301 ctcgcaggta tctaagtggt gcggtattat agggacaatt attccagcta ttatccttgt
    84361 tattcttggt ttctcctatc tgtggttcag cggtaaacca gcggtgatcc cattaggttg
    84421 gggtgatctt ttccctaata tagctaactt ccagaatatt gttcttgccg ccagcatatt
    84481 tcttgcttat gcagggatgg aaatgaatgc ggtccatgtt aacgatttag ataatccaac
    84541 taaaaaatac ccaattgcaa ttaccatcgc ttctataggt actattgcaa tattcctgct
    84601 cagtacttta ggggtcgcct ttattattcc tacagataaa attagtctaa ctcaaagttt
    84661 gttggttgcc tacgatatgc tgttcgaatg ggctggagtg ccatggcttg gctctgtgat
    84721 ggcatttatg ctagcaattg gcgtactggg tggggtggtt acttggattg cagggcctaa
    84781 tacaggtgta ttagcgatcg cgaaagctgg ttatttacct aagtttttcc aaaaaaccaa
    84841 tcgtcatgga atggggcacc atctaatgtt tgtacaaggt ataattgtct cagttctttc
    84901 tgttactttt gtgataatgc cttcagttca agcagctttt cagattttat cacagttaac
    84961 agttatgctc tatttagtta tgtatatgct catgttcgct agtgctatct atctacgaca
    85021 ttctcaacct gatacaaaac gcccatatcg agcccctgct ttatttttct gggcaacact
    85081 tggattctta gggtcttttc tggcttttgt attaagtttt attccgcccg agcaaatccc
    85141 aactggtagt ccaaccagct atgttcttta tttgttagta ttagttgccc tatttttatt
    85201 aattccaata ttaatttacg ccttccgaaa atctgactgg aaagatccaa actctgattt
    85261 cgaaccattt tcttgggaaa aaagaactag taattaaatt ttccataagc tacaaaagat
    85321 gaggttaaat atgtttagtg ctgaatctcc aattggtgta atagactcag gtgtaggtgg
    85381 attgacatcg gtaaaggaaa taattaattt gcttcctggt gaagatatta tctattgtgg
    85441 agataatatg aatgcacctt atggcaaccg ttctgccgat gatatcatca gtttgactaa
    85501 aaaaatgctg acttttttac agtcaaggaa tgtcaaacta gttgctgtcg catgtaatac
    85561 tatatcctca actttggaat ccgaaaaata cagcggatat gctaagagct ttccgtttcc
    85621 tatcttatcc atcattgaac ctgcagtaga agatgtgatt cgccagcaat ataaaaatgt
    85681 tggtattatc gctaccgagt ttactattaa aacggggtgt cacaaagagc taataaaaaa
    85741 attgaattca acaataaatg tatttggaga accaagcaaa aatcttgcaa tgttaattga
    85801 ggaaggtaat cttaatgctc ctgcaatctt gaatgacatc aaaaaacatg ttaaccattt
    85861 aatttccctc catccagtaa atgaaattat tctcggctgt actcactatc caattgttca
    85921 gaattttttt gaagcagttg caccggatat aaaatttatt aatcctgcac atgatcaggc
    85981 tatctctatt aaaaatcact tgggacagct aaatttatta aactcctcaa atataggaac
    86041 tttgcaaata aatactagtg gaagtatgga gatctacaaa acggtactag gggaattgag
    86101 cattacgaaa cctcatacat tcagtatcag gcagttttaa atttatccgg tgcaccgtaa
    86161 aatcccgtcc ttctgaactg caccccaaaa gttggacacc aactgagtaa ggtgcatttt
    86221 atggacaaac ccagttacat actgggtagt gcatccaatt agtagaacat gtgtttttcg
    86281 ataaacgctc cgatcacttt ttcgtggatc tcaactgagc gtgagaagca gattgtttta
    86341 cgacccaacc gcttaatgcg ggtgcgtagc gtcagattat tacgctcaat gcgttgggtg
    86401 aatattttgc cggtcagatg cttattcttc ggcacctccc ggccatagct gccccagtca
    86461 tcgctggtca gcatgccgat gttgaagggt ggcagtgcca gtagctcccg gcacgtttga
    86521 tcggttcggg gaccaaaagt gtaggccagt acaccgcctg ttttggtgtt gtacgcgtac
    86581 ccgagccaat gttgccgggc tttactgcca acgtagctcc attgctcatc aagctcgcag
    86641 ataaacgcca catcagcatg ggcaacaggc gaagaagtta ttcgctttgg cgcgagtttt
    86701 ttaaagtccg gatgacggtg ttactaccaa ttttcagtgt cctggcggta tcgcgaaccc
    86761 cggcaccatt aaaggccatt tcagttatca gctctttcat acccggctta cgtgcttgat
    86821 aagtataagt gaactgaaac acacggtggc agtcacggca gcgaagtctg tcacggcctt
    86881 tagggttctg accatggcgg taaacctgag ctgactgaca acggggacaa tgaatattaa
    86941 cgctggtcat gagataacct caaaagcccg tattatacat cagattcaac taattagagg
    87001 catcacccat ccagcattat aaaacatact aacaataact aaattataat aatcacaact
    87061 tacaatcaaa taataaatgc ataagattaa caatgatttt ttcgggttat atttatctaa
    87121 aaaagcagaa tattatgtaa cttattattt tctaagcgaa aatttattgt gcagtcagac
    87181 tagcaaactt gcgtaccacc cgaagaacat gcgaagattt aagtattctt tcttttttgg
    87241 tttttatttt tcattttctc ttttcttctc tttccggttc tcttctgttc tggttttggt
    87301 tttttccttt ttatttttat tctttcttca gggttatgct aaaacaccaa aacatttttc
    87361 accacaaaag catccaatca tcttgccaga ccaatcacct taaccggata cactgccgga
    87421 tataatccca ctaacaccca gccaccaagc accatacctg ataactaccg tattcaggaa
    87481 ggaaatgcga gtgatttcac cgtcaccggc acaaccgatc cgccaaaaag aggcgtacca
    87541 atgtcgatat agtcccccgc gcggacaatc acttcgtcaa tgactgccaa cgggagttgt
    87601 tgtagctgtg ggcgcaacaa catgcccaga gctttaccaa agtcctgccc ggccaccacc
    87661 agcaggggat gcggattcgg aaaacgcgcg acgaaatcga ccagcgcgtt gatgaccgtc
    87721 agtaccgcgg cgtaacgcac aggcagcgag gcgggaagcg ccagcacgta cacgtcagtt
    87781 ttgggatcaa gatccagctg aagcagcgcc tgttgccagg cactcaccag atccgtttca
    87841 tcaatcggga tcgccaccgg caaattgcgc agcggcagtt gtacgccctc cagccagatt
    87901 gtgctgccag agagcgaaag ggtatgtgca cccgcgccaa tcaccgtggc gcatacggtt
    87961 tgcgccggaa actgcacatt catctcacgc aggcgcggat ggtcatgcag cgccgttgcc
    88021 agaagtgggc caatatcggc aaaacagaat gggtcggcgg gctggtggcg ataacattcg
    88081 cccacacctc cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag caaaccggtt
    88141 tgcatcaatg cctgcgcgag cggcgaaagc gttccgtcaa tcacttcgac aatcagcgcc
    88201 gccatccgcc gggtaacctg caccagctgc gcgccggtca gcgaacgggc gtcagtgcct
    88261 gcaccgaagc actcatccac aatcatctgc cccggtttat gagcgtaaac cacgcgcccc
    88321 tggctgtcgg tttccagcag gcgaccaccg acgttgagac aggcagtgcc gctgattttt
    88381 ccggcatcga acagggcgta gttcgcggtg ccaccgccga tatcgatatt cagtacccga
    88441 cacagccgtt gttcagaaag ggtttgcgcc ccggctccgt gaccggcgat cacggattcg
    88501 aggtgcggcc cggcgctggc gacgacaaaa tcgcccagcg actgtttttc cttcatcact
    88561 ttttccatca gctgtgcgac caggctttcg gtaaactggc cttccggcag ctgggcaatg
    88621 atggtttcgc ggatgcgctg gctttcggtt ttgtcggctg gcacgctggc gggcacggac
    88681 gccgttaccg ggatcgattc atcacattcg gtgatggtaa aacccagtag atccgacact
    88741 tcacgggctt ccggggtaat aatgctggcg cgcagaacca cggacattgc ctgttcgccg
    88801 cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt ttttcacctg cttcctcctg
    88861 tttggtgaga gatgccagat aatccaccag ttgctgcacg ctttgcgggt cgtggctgtt
    88921 tagctcgaaa atcggctctt cgaaacccgt ctcaagcaat aactttcgcg ttgcggcgac
    88981 gtcagcatct ggcatgtccg ttttactgat gacggcgatt tgccgtttgc tgacgccaat
    89041 atccagcaac ccggcaggca accgactttc cgggtcattt gcgccatgga catagatcag
    89101 catgtctaca tcctgtagcg tggtaattaa ggcgtgatac cagcggggat ggctaaaata
    89161 ttcacccggc gtatcaatat cgcccttatc attaaattcc acggcctgtg tttttctggc
    89221 gagggtataa ttcccctgca acgcattaaa taacgttgtt tttcctgccc cgacgctacc
    89281 cacaaacgca attcgtttca tgaatgcctc gtattaactt ttggtcattt cacaaagcgt
    89341 ataatttaat aagcgaccta aaccgctgac ggtttgtgat aacgcttctt ctactgcgcc
    89401 caccgagcca taaatcacca atgcaccgct gaagcggtcg agaaaaccga tatgcacatc
    89461 agcggctttc agcgctaaat caccggcgat catcgccgtt tcgccgggag ttagcgtcat
    89521 aatgccaatt gcgcccgcat ccggaacgcc gattttcttc gccagttcct caccgggatg
    89581 cgcgatgaga tgcgccagtg tgacctgttt gccgggcaca aattcctgaa tgatgcgttc
    89641 tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat cgtgacaatt tttcgtcaga
    89701 gtaaataaca aaattcggca agcgcgttta aaaggtgagg tagatcacta agaatggaga
    89761 gatatttact gttatgaata aactttcata tgcgacgcat gaatataaat aaaataatat
    89821 cgagatattt tatatactga tttttttatt attcgcaaac tcagtaataa gctttgctaa
    89881 tatatttcag gaaaaccgct taccctgaat attccgggta agcgtgagag ttaagaatga
    89941 attacagcgg ttgggtttgc gcttcaacca cggccagcgc caccatattg acgatacgac
    90001 gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc cacgcccatc agcaccggac
    90061 cgacagtcac accttccgag ctggaaacgc gcagtaagtt gtaactaatg cgggcagctt
    90121 ccatgttcgg catcaccaga atattggcag aacctttcaa agggctgtcc ggcatacggt
    90181 cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat ttcaccatca atcatcagat
    90241 ctggcgcacg atccctgacc agttccagcg cctgacgcat tttgctcgac gacgggcagt
    90301 cagaagaacc aaagttggag tgcgacaaca gagcaacgcg cggctcaata ccaaaacgac
    90361 ggacagtttc tgccgccatc aaggtgatct ccgccagctc ttctgcgtct ggttcgtcat
    90421 tgacgtaggt atcggcaaca aaggtgttac cactcggcag cagcagcgcg ttcattgcac
    90481 ctgcggtgtg aacgccatcg cgataaccaa agacattttt caccacgcta aaatgttcat
    90541 gataatcgcc caccgtaccg cagatcattg catcggcttc cccacgctga accatgatcg
    90601 cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc ctgttcctga gtgacaccgc
    90661 gacgcttcat gatctggaag tattcggtcc agtactcttt aaagcgcgga tcggattcgt
    90721 tattgacgat ttcaaaatca acgcccgctt tgatctgcaa gcccagtttc tgaatgcgca
    90781 tttcgatcac gttcggacga ccgataagga tcggtttcgc cagtcccagc gttaccagtt
    90841 cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg cagaactacg cgcttcggcg
    90901 ctttgcgagc ctgggagaaa atcggcttca taaacaggtt ggttttgtaa acgaactcag
    90961 tcagcttgtc gatgtagacg tcgaaatcag caatcggacg cgtcgccacg cccgactcca
    91021 tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa gcgcggatca aacggttttg
    91081 gaatgatgta ttccggacca aagctcagat cctgaacgcc atacgctgaa gccaccactt
    91141 cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac cgccgccagt ttcatctctt
    91201 cgttgatggc ggttgcgcca acgtcaagcg cgccacggaa gatgaacggg aagcaaagaa
    91261 cgttgttcac ctggttcggg tagtcggaac ggccggtaca gatgatggca tccggacgca
    91321 cttctttcgc cagcggcggc agaatttccg gttccgggtt cgccagcgcc aggatcattg
    91381 gtgcccgagc cattttcttc accatttcct gggtcagcac tttcgggccg gaacagccca
    91441 ggaaaatatc cgcaccttca atcacatcat cgagggtacg tttgccgtca tccaccaccg
    91501 catatgcggc tttggtttcc gccatatttg gctcacgccc ctgatagata acgccttttg
    91561 aatcgcaaac cacgatgtta tgtttttgca gccccagcgc taccagcagg ttcatacagg
    91621 cgattgctgc ggcacccgcg ccggaaacca ccatccgcac gtcggagatg tttttctcca
    91681 ccacgcgcaa gccgttgagg atggcggcag tgctgatgat tgccgtgccg tgctgatcgt
    91741 cgtggaatac cggaatattc atccgctcgc gcagtttctg ttcaatatag aaacattctg
    91801 gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg ttcgagcgcg gcgacgactt
    91861 caataaattt gtccgggtcg agttcatcaa cttcaatgtc aaatacatca atcccagcga
    91921 atttcttaaa tagaacgccc ttgccttcca tcaccggttt gcctgccagc gcgccaatgt
    91981 tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc aaccaggtta ccgcgcgcgg
    92041 tatatttgta ggcttttagc gggtcttttt cgatttcaag acaaggtgcg gcaacgcctg
    92101 gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg cttggttgga gaaacctgga
    92161 ttttccctgg aactggaaat tcatggaaat caagtgcact ttgttttaac tggtcatcca
    92221 tttggttgtt cctttcacgt aacgttcaca aataaagtgt gtgggcaaca gcccctgccc
    92281 acaacgtggc gcacattatt accctgccgg agtctacaga ctttgagcaa gtccaaactc
    92341 tcaccattaa tataatgttt tggtaacaat cctataacac tgatattacc tgcttaatcc
    92401 agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt tagcgaaatt tctcagaagt
    92461 gtgaattaac gcactcatct aacactttac ttttcaagga gtatttccta tgaacgagtt
    92521 agacggcatc aaacagttca ccactgtcgt ggcagacagc ggcgatattg agtccattcg
    92581 ccattatcat cccctggatg ccaccaccaa tccttcgctg ttactcaagg ctgccggatt
    92641 atcacaatat gagcatttaa tagacgatgc tatcgcctgg ggtaaaaaaa atggcaagac
    92701 ccaggaacaa caggtggtcg cagcgtgtga caaactggcg gtcaatttcg gtgctgaaat
    92761 tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat gcacgcctct cttttgataa
    92821 agaaaagagt attgagaagg cgcgccatct ggtggacttg tatcagcaac aaggcgttga
    92881 gaaatcacgc attttgatca aactggcttc gacctgggaa ggaattcgcg cggccgaaga
    92941 gctggaaaaa gaaggtatta actgcaacct gacgctgctg ttttcttttg cccaggcgcg
    93001 ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt gtagggcgta tttatgactg
    93061 gtatcaggca cgcaagccga tggacccgta tgtggtggaa gaagatccgg gcgttaaatc
    93121 ggtgcgcaat atctacgact actataagca acaccactat gaaaccattg tgatgggcgc
    93181 gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc tgcgatcgac tgactatcgc
    93241 accgaatttg ctgaaggagc tgcaggaaaa agtttctcca gtggtacgta aattaatccc
    93301 gccttctcag acgttcccac gcccagcacc catgagcgaa gcggagttcc gttgggagca
    93361 caatcaggat gcgatggcgg tagaaaaact gtctgaaggc attcgtctgt tcgctgttga
    93421 tcaacgcaaa ctggaagatc ttcttgccgc caaactataa accagccacg gagtgttata
    93481 tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag tatggatgcg gtacaaaaag
    93541 ccaattctgg tcatcccggc gcgccgatgg gcatggctga tattgccgaa gtgctgtgga
    93601 acgattttct taaacataat cctaccgacc caacctggta tgatcgcgac cgctttattc
    93661 tttccaacgg tcacgcgtcg atgctgctct acagtttgct acatctgacc ggttacgacc
    93721 tgccgctgga agaactgaag aacttccgtc agttgcattc gaaaacccca ggccacccgg
    93781 agattggcta tacgccaggc gttgaaacca ccaccggccc gcttggacaa ggtttggcga
    93841 acgccgtcgg gctggcgata gcagagcgta cactggcggc gcagtttaac cagccagacc
    93901 atgagatcgt cgatcacttc acctatgtgt ttatgggcga cggctgcctg atggaaggta
    93961 tttcccacga agtctgttcg ctggcaggca cgctgggact aggcaagctg attggttttt
    94021 acgatcacaa cggtatttcc atcgacggtg aaacagaagg ctggtttacc gacgatacgg
    94081 caaaacgttt tgaagcctat cactggcatg tgatccatga aatcgacggt cacgatccgc
    94141 aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa agataagccg tcgctgatta
    94201 tctgccgtac ggtgattggc tttggttcgc cgaataaagc aggtaaggaa gaggcgcacg
    94261 gcgcaccact gggggaagaa gaagtggcgc tggcacggca aaaactgggc tggcaccatc
    94321 cgccatttga gatccctaaa gagatttatc acgcctggga tgcccgtgaa aaaggcgaaa
    94381 aagcgcagca gagctggaat gagaagtttg ccgcctataa aaaggctcat ccgcaactgg
    94441 cagaagagtt tacccgacgg atgagcggtg gtttaccgaa ggactgggag aaaacgactc
    94501 agaaatatat caatgagtta caggcaaatc cggcgaaaat cgctacccgt aaggcttcgc
    94561 aaaatacgct taacgcttac gggccgatgc tgcctgagtt gctcggcggt tcggcggatc
    94621 tggctcccag caacctgacc atctggaaag gttctgtttc gctgaaggaa gatccggcgg
    94681 gcaactacat tcactacggg gtgcgtgaat ttggcatgac cgctatcgcc aacggcatcg
    94741 cgcaccacgg cggctttgtg ccgtataccg cgacgttcct gatgtttgtt gaatacgccc
    94801 gtaacgccgc gcggatggcg gcactgatga aagcgcggca gattatggtt tatacccacg
    94861 actcaattgg cctgggcgaa gatggtccga cgcaccaggc tgttgagcaa ctggccagcc
    94921 tgcgcttaac gccaaatttc agcacctggc gaccgtgcga tcaggtggaa gcggcggtgg
    94981 gctggaagct ggcggttgag cgccacaacg gaccgacggc actgatcctc tcaaggcaga
    95041 atctggccca ggtggaacgt acgccggatc aggttaaaga gattgctcgt ggcggttatg
    95101 tgctgaaaga cagcggcggt aagccagata ttattctgat tgccaccggt tcagagatgg
    95161 aaattaccct gcaagcggca gagaaattag caggagaagg tcgcaatgta cgcgtagttt
    95221 ccctgccctc gaccgatatt ttcgacgccc aggatgagga atatcgggag tcggtgttgc
    95281 cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat tgccgattac tggtacaagt
    95341 atgttggtct gaaaggggca attgtcggga tgacgggtta cggggaatct gctccggcgg
    95401 ataagctgtt cccgttcttt ggctttaccg ccgagaatat tgtggcaaaa gcgcataagg
    95461 tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc tgtatgttcc cctcacccta
    95521 accctctccc cagaagggcg aggggacggt ccgtgcgctt tttagggccg catctgcgat
    95581 tttgtttaac gggtgatcac aacgtgggcc tggcgtctgg cccatgccag ttatcgcagg
    95641 taggttctgc ggcgtaacgc accaggcgaa aactctgacc gtcaaagcgc cagcgcgcct
    95701 gaatgccaca atcgcttaat ccgcgccctt tcgctaaggt caccagttca cgcgatttct
    95761 catcaaatgt tgcgttcatc agttccagtt cattcgtctc ctgaccgttg ttgaacggca
    95821 aacgcaaccg aaccgggcgc gaggctagcg gctttttacg cgacacaatc catgccaaat
    95881 caatggtgtt ataggcccct gcctcacagc taatcatcat cagtgcttta tcatcggtca
    95941 gcgcagtgac attcacctca cgacgtaacg gatcaagcga gcagcgcaga ccattcatcc
    96001 gccagtttcc ataatccagc aaatcgttac gttcttcgag tgagagtggc gtcggcgttg
    96061 ggttaaccac cgcgacctct ttcagcgcag gcgcgggcgg tacgctgaga ggcggttcgt
    96121 cccctttctt gatccacgcg gtttcactgc caacgcgctt ttgctgagca tcaataaaca
    96181 acaacgccgc ttttaagccg ctcagagaaa tggtctgatt gccgtcgcgt aaggtgatcg
    96241 ccttcccttc ctgaattatc tgcaaaaacg cggtgatggt tgccgtatca tcggttacta
    96301 ataaccatgg tgaaatccgc cacttgtcac cacttaatgc caacggctca ccatctaata
    96361 gcagccgtgg cgctatctcc ccttcagacg catccggcga cttcaaccca ccgcgctcaa
    96421 tacgtaaaac ggcatcggta tgcgccccag cgctgcggct cagggtcatc accagtccat
    96481 tatgatcgcc cgtattgcgc gccacacaaa aattttgatt attacaggtg acctgccagt
    96541 cggaaaacgc ccgttgcgct ggtgccgccc acaccaaaga cgtcggcaac aaagcgaaaa
    96601 aaaagagaag gaaaatgcga tagcgcatgg acggtacgac cccagaagtt caaacaaaca
    96661 gcacaagtcg tatcttcgtt gcgcaggtag cgcggctcaa tcggatttgt cggattgagc
    96721 caataataaa caacgaaata ttattgctat ttttcagtcc attaaatgtg acatttgcaa
    96781 atagttaagc aataacaccg tcttaccgtc acgtatctcg ccggttttga tcatctccag
    96841 cgcctggctg aacggcagct cgagcacttc aatatcttca tcttcaacac cgccaccggc
    96901 gttagcgcgc tgattgtcac tgtattcggc gataaaaaag tggattagct cagtcacacc
    96961 gccaggcgac atatacagtt caaataattt gcgcacttcg cccacttcat agcccgtctc
    97021 ttcaatcgct tctttacgaa tgcacacttc cggttcgtcg ttatccagca gcccggcgca
    97081 ggtttcaatc agctgcccgc tttcattgcc attaacccag gtagcgacac ggaactgacg
    97141 aatcagaacc acgctctttt tcttcgcgtt gtacaggagg atcgtcgcac cattaccgcg
    97201 atcgtaaact tcacgtttat ggcggataac ttcaccatct ttgcgcgtga gatcgtaagt
    97261 aatgttgtgc agggtgaaat agttatcgga gagaattttg tctttaatga gggtgatttg
    97321 ttgcgtcata ccgactccac agcgcgaaat gaacaattat attacgctgt gaagtcgggt
    97381 ttgtctgcgc aggctatcag tgagatttga ctgattttac ccccagccag tcaataatcc
    97441 cctgtgccgc atgacgtcct tctgccattg cggtaaccac cagatccgca ccacgcacgg
    97501 cgtcgccacc ggcgaagatt ttcggattgg tggtctggta acggtactgg ctttccacat
    97561 ccgcgatgat gcggccccat ttgtctaccg ttacaccgtg cgactccagc cacggcatcc
    97621 cgtgtggatt gaagccaaac gccataatca ccgcatcggc tggcatgaca aattcactgc
    97681 cctcaatcgg tacaggtcgc cgacgcccct gggcatccgg ctcgccaaga cgcgtgcgca
    97741 ggaaacgaat tccgcaaacg tgaccttgct cattcagctc aagcgccacc ggctggacgt
    97801 taaattcgaa gttggccccc tcttcgcggg cgttcttcac ctctttcttc gagcctggca
    97861 tgttagcttc atcacgacga taagcgcagg tgacgttact cgcgccgtgg cgcagcgcag
    97921 tacgcacaca gtccatcgcg gtgtcgccgc cgcccagtac cacgacgtta agtccggcgg
    97981 tattgataaa cggctcttcc ggtagctctt cgagccccat cacctgttta gtgttggcaa
    98041 tgatgaacgg cagcgcgtca taaacgcccg gcgtatcttc attgggtaaa cccgctttca
    98101 tggaacggta agtgcctacg ccaacgaaga ccgcgtcgta ttgttccaaa agcgaatcca
    98161 aagagacatc tttacccact tcacaattga gttcgaagtg aatccccatc gcgctgaaga
    98221 tttcccgacg gcgtgccagc agggatttat ccagtttgaa agaaggaatg ccgaaagtga
    98281 gcaagccacc gatttctgga tggcgatcgt acaccgtcac cgccacgcca ttgcgggtca
    98341 gaacatccgc acaggccagc cctgccggac ctgccccgat aatcgccacc cgcttgtcca
    98401 ctttggtgac atgacttaag tcaggacgcc aacctttcgc caacgcctga tctgaaatat
    98461 agcgttcaat gttgccgata gttaccgcgc cgtgctcatc gcgaatagta caggcacctt
    98521 cacacaaacg gtcttgcgga caaacgcgtc cggtaatttc cggcagggtg ttggtctggt
    98581 gagaaagctc gacggcggcg tcgatgtttc cggctttcac cagttcaatc cactgcggaa
    98641 tatggttatg cagcgggcag gtccattcac aaacgctgtg ctcgccgaac ttaaggcagc
    98701 gcgaggcttc ccgttgtgcc tggtcggcgc gaaatggcag ataaatttca tcaaaaccgg
    98761 ttttgcgcgc ttcaatcgcc agtttatccg gctcgccacg cgcgggcgtt gcctgcattt
    98821 gttcgacttt actcattacc ggcatttctt gtgcagcggt actggcatgc cacggttgat
    98881 gctcctgacg tgcggtgcgc aagcggcggg atttcgccat gccggagagt gcgacgtcag
    98941 tgaccagttg cagcgcgtcc gccgggcaat tctcgacaca ggcaggaccg ttttcgcgcc
    99001 ctgcacaaag gtcgcattta tgcgccgtgg cttttacttt tcctgccgcg acgggtgtca
    99061 ggacgatttg catcgtacca aacggacagg ccaccacgca ggatttacag ccaatacact
    99121 tttgctgatt gacctgaatg ctgtcatcaa cgtggctgat tgcgccatta gggcagctac
    99181 gggcgcaggg cgcatcttca caatggtgac aggtcactgc actacgttgc tgctgatgtt
    99241 tgataaccgt aattcgggga tgaaaatggt gttggctcag gacatgttgc tcatcattgt
    99301 gagccatgac acaggcgatt tcacaagcat gacaacccag acattgctga ctgttggcca
    99361 taataaaacg attcataacg accttctttt ttggttgtaa aaaccttatt ctttatatga
    99421 gtgttgttat tacccgactt acaggggaac cggcaatgtt catgtgccca gaataagtaa
    99481 ctatttcgct ataaactgtg gcagatcaaa taatcccatc actgactaaa ttgcgtttca
    99541 gcgaactgga acattaatga ttttttgtgg agaagacgcg tgtgattgtt aaacgccccg
    99601 tctcggccag tctggcccgg gccttttttt acattgtgct gctgtcgatt ctttccacgg
    99661 gtatcgctct gctaactctg gcgagcagtt tgcgcgacgc tgaggctatc aatattgccg
    99721 gatcgctgcg tatgcagagt taccgcctgg gctacgactt gcaaagtggc agtccacaac
    99781 tcaatgcaca tcgccagttg tttcagcagg cactgcattc accggtatta accaacctca
    99841 acgtctggta tgtgccagaa gcagtaaaaa ctcgctatgc gcatctgaat gccaactggc
    99901 tggagatgaa taatcggctc agcaagggcg atttgccgtg gtatcaggcc aatattaata
    99961 attatgttaa tcagatagac ctgttcgtgc tggctttaca gcactacgct gaacgcaaaa
   100021 tgctgctggt ggtggcgatt tccctggctg gcggtatcgg tattttcacg ctggtctttt
   100081 ttactttgcg ccgcatacgc catcaggtgg ttgccccgct gaatcagctg gttaccgcca
   100141 gtcagcgtat tgaacacggg cagttcgact cgccgccgct ggataccagt ctgccgaatg
   100201 agcttggtct gcttgcaaaa acctttaacc aaatgtcgag cgagctgcat aaattgtacc
   100261 tttcgctgga ggcgtcagta gaagaaaaga cccgcgatct ccacgaggcc aagcgtcgtc
   100321 tggaggtgtt gtatcagtgc agtgttccca ggcgctgaac accagccaga ttgatgtgca
   100381 ttgtttccgc catattttgc agattgttcg cgacaatgaa gcagctgaat atctggagct
   100441 gaacgttggg gataactggc ggattagcga agggaagcca aaccctgagc agccaatgca
   100501 gattttaccg gtgacaatgc aagagacggt ttacggcgaa ctgcactggc aaaatagtca
   100561 cgtttcatca tcagaaccgc tgcttaacag tgtttcatcg atgctgggac gcggtttgta
   100621 ctttaatcag gcgcagaagc attttcagca gttattgttg atggaagaac gtgcgaccat
   100681 cgcccgcgaa ttgcacgact cgctggctca ggtgctttct tacttacgta tccagttgac
   100741 gttactgaag cgttcgatac cggaagataa cgccaccgca caaagtatca tggccgattt
   100801 ttcccaggcg ttgaatgatg cttatcggca gttacgcgag ctgttgacta ctttccgcct
   100861 gacgctgcag caggcggatc tcccctccgc gttgagggaa atgctggata cgttacaaaa
   100921 tcaaaccagc gctaaactga ccctcgactg ccgtctgcca accctggcac tggatgcgca
   100981 aatgcaggtg catttgttgc aaattattcg cgaagcggtg ctgaatgcga tgaagcacgc
   101041 caacgccagc gaaatcgcca tcagttgcgt caccgcgccg gacggcaatc acacggttta
   101101 tatccgtgat aacgggattg gtatcggtga accgaaagaa cccgaaggtc attatggact
   101161 gaatatcatg cgcgaacgcg cggaacggct gggcgggacg ctgacttttt cacaaccttc
   101221 cggcggcggc acgttagtga gtattagctt tcgctccgca gagggtgagg ggaaggggaa
   101281 ggggaacacg ccggctgagg tgtaaataat taccctcaca ccggccctct ccctttaaag
   101341 gcgagaggga aaaacgtgcc catattgacc tgtaagcgag tgtgtcagca atctcatgat
   101401 aatttacatt aactcctttt tttctccacg attggctcgt accttgccgc tacagtgaag
   101461 caagtcaagc ctacaacaat acgcagaaac acgaggtcct cttttaatgg cgaatttctt
   101521 tattgatcgc cccatttttg cctgggtgct ggcaatcctg ttgtgtctga caggtaccct
   101581 ggcgattttt tcattgcccg ttgaacaata ccccgatctc gcgccaccga atgtgcgagt
   101641 gaccgctaac tatcccggcg catcggccca gacgctggaa aacaccgtga cccaggttat
   101701 cgagcaaaat atgaccggcc tcgataatct gatgtatatg tcatctcaga gcagtggcac
   101761 cggtcaggca tctgtcactt taagttttaa agcaggcacc gatccggacg aagccgtgca
   101821 gcaagtacaa aaccagctgc aatcagccat gcgaaagtta ccgcaggcgg tgcaaaatca
   101881 gggcgtgacg gtgcgtaaaa ccggcgatac caacattctg accattgcct tcgtctctac
   101941 cgatggctcg atggataaac aggatattgc tgattatgtt gccagtaata ttcaggaccc
   102001 gttaagccgc gtgaatggcg tcggggatat cgatgcctat ggttcgcaat attccatgcg
   102061 tatctggctg gacccggcga aactcaacag tttccagatg acggctaaag atgtcactga
   102121 tgccattgag tcacagaacg cgcagattgc ggttgggcaa cttggtggta caccttccgt
   102181 cgataagcag gcgctcaacg ccgccattaa cgcccagtca ctgctgcaaa caccagaaca
   102241 gttccgcgat atcaccttgc gggtgaatca ggacggctca gaggtaaggc tgggcgatgt
   102301 cgccaccgtc gaaatggggg cggagaaata cgattatctt agccgcttca atggtaagcc
   102361 agcctccggg ttgggggtaa aactggcctc cggcgcaaac gaaatggcga cagcggagct
   102421 ggtgctcaat cgtctcgacg agctggcgca gtatttcccg cacggactgg aatacaaggt
   102481 ggcgtatgaa accacctcgt ttgttaaagc ctccattgaa gacgtggtga aaacgctgct
   102541 ggaagctatc gctctggttt tcctcgttat gtatctgttc ctgcaaaact tccgcgccac
   102601 gctgataccc actatcgccg tgccggtggt gttgatggga accttctccg tactttacgc
   102661 cttcggttac agcgtcaaca ccttaaccat gttcgcgatg gtgctggcga tcggtctgct
   102721 ggtggatgac gccatcgtgg tggtggaaaa cgtcgaacgt attatgagtg aggaaggact
   102781 cactcctcgc gaagccacac gtaaatcgat ggggcagatc cagggggcac tggtcgggat
   102841 tgcgatggtg ctttcggcgg tatttgtacc aatggccttc ttcggcggca ccaccggtgc
   102901 catctatcgc cagttctcta ttaccattgt tgcggcgatg gtgctgtcag tactggtagc
   102961 gatgatcctc actccggctc tgtgtgccac gctacttaag ccactgaaaa aagctgagca
   103021 tcacgggcaa aaaggttttt ttgcctggtt taaccagatg tttaaccgca acgccgaacg
   103081 ctacgaaaaa ggggtggcga aaattctcca ccgtagcctg cgctggattg tgatttatgt
   103141 cctgctgctt ggcggcatgg tgttcctgtt cctgcgtttg ccgacgtcgt tcttaccgct
   103201 ggaagaccgg ggcatgttta ctacctcggt acaattgccc agcggttcaa cccaacaaca
   103261 gaccctgaaa gtcgttgagc aaatcgagaa atattacttc atccatgaaa aagacaacat
   103321 catgtcggtg tttgccaccg ttggttctgg ccctgggggt aacgggcaaa acgtggcgag
   103381 aatgtttatc cgcctgaaag actggagcga acgcgacagt aagaccggca cctcgtttgc
   103441 cattatcgag cgtgcaacga aggcgtttaa ccaaattaaa gaagctcgcg ttatcgccag
   103501 cagcccacca gcaattagcg gtcttggtag ttctgcagat tttgatatgg agttgcagga
   103561 ccacgctgga gcgggtcacg atgcgctgat ggcagcacgt aatcagttgc tggcgctggc
   103621 ggcggaaaac ccggagctaa cccgtgtgcg ccataacggc ctcgatgaca gtccgcagtt
   103681 gcagattgat atcgaccagc gtaaagctca ggcgctgggc gttgatatcg acgatattaa
   103741 cgacacactg caaaccgcct ggggttcgag ctatgtgaat gactttatgg atcgcggtcg
   103801 cgtgaagaaa gtctatgtgc aggcagctgc gccgtatcgc atgctgccag atgacatcaa
   103861 tctctggtat gtccgaaata aagatggcgg catggtgccc ttctctgctt tcgcgacctc
   103921 acgctgggaa acaggctcgc cgcgtctgga acgctataac agttattctg cggttgagat
   103981 tgttggggaa gccgcaccgg gggtcagtac cggtacggcg atggatatta tggaatcgtt
   104041 agtgaagcag ctgccaaacg gctttggtct ggagtggacg gcgatgtcgt atcaggagcg
   104101 gctttccggc acgcaggctc cggcgctgta cgccatttcc ttgctggtgg tattcctgtg
   104161 tctggcggca ttgtatgaaa gctggtcggt accgttctcg gtaatgctgg tcgtgccgct
   104221 gggggtaatc ggcgcgctgc tggcaacctg gatgcgcggg ctggaaaacg acgtttactt
   104281 ccaggtgggc ctgttaacgg tcattggttt atcggcgaaa aacgccatcc tgatcgtcga
   104341 gtttgctaac gagatgaacc aaaaaggcca cgacctgttt gaagcgacgc tccacgcctg
   104401 ccgtcagcgt ttacgcccga ttctgatgac ctcgctggca tttatcttcg gcgtattgcc
   104461 aatggcaacc agcacgggtg ccggttccgg tggtcagcat gcggtgggta ctggcgtaat
   104521 gggcgggatg atttcagcta ctattctggc tatttacttc gtgccgctgt tctttgtgct
   104581 ggtgcgccgc cgcttcccgc tgaagccgcg cccggaataa gcaataaaaa aggcgacatg
   104641 ccaatgtgtc gcctttttca actttccgat taagaacctg ctcagcgggt tcttgctgtt
   104701 tatactttgt ctcaggaatt acttacgaag cataacttcg ataaagtctt tccagttccc
   104761 cagttcacgt tcaatcataa caacctctct tataattatg ggtattctac ggaaaaaata
   104821 taccgtggtg aagctaattt actcgattgc tgcgatgact acctccgggg gacaaacctt
   104881 atgtaaatac tatggtcctg cggtgataat ttgtatgtga tacacagcaa catttcgaga
   104941 tattcatacg gcatctaata cttatttaat tctggttaaa atacagacag ataacaagat
   105001 gaatattctt aatgtttacg ttaaaaatgt ttaatattat ttaatagttg ttaatttgaa
   105061 tacttcgata atgttatatt ttctgataat catttgcagg caaaatgttt tcacccttaa
   105121 atgagtattt attctcataa atcgaaaaag gattcattat ggttacactt tacggcatca
   105181 aaaattgtga caccattaaa aaggctcgcc gttggctgga agccaataac atcgactatc
   105241 gttttcatga ttaccgcgtc gatgggctgg acagcgaatt attgaacggt tttatcaacg
   105301 aattaggctg ggaagcgtta ctcaacaccc gtggtacaac ctggcgtaaa ctggacgaaa
   105361 ctacccgcaa taaaatcacc gatgcggcct ctgcggcggc attaatgact gaaatgcctg
   105421 caattatcaa acgtccattg ctctacgcgc ctggtaagcc tatgctgctg ggtttcagtg
   105481 attccagtta tcagcaattt ttccatgagg tgtagtctat gtcgtgcccg gttattgagc
   105541 tgacacaaca gcttattcgc cgcccttccc tgagtcctga tgacgcagga tgtcaggctt
   105601 tgttgattga acgtttgcag gcgatcggtt ttaccgttga acgcatggat tttgccgata
   105661 cgcagaattt ttgggcatgg cgtgggcaag gtgaaacgtt agcctttgcc gggcataccg
   105721 acgtggtgcc gcctggcgac gccgatcgtt ggatcaatcc gccgtttgaa cccaccattc
   105781 gtgacggcat gttattcggg cgcggtgcgg cagatatgaa aggctcgctg gcggcgatgg
   105841 tggtagcagc agaacgtttt gtcgcacaac atcccaacca tacggggcga ctggcatttc
   105901 tgatcacttc tgatgaagaa gccagtgccc acaacggtac ggtaaaagtc gtcgaggcgt
   105961 taatggcacg taatgagcgt ctcgattact gcctggtcgg cgaaccgtcg agtatcgaag
   106021 tggtaggtga tgtggtgaaa aatggtcgtc gcggatcgtt aacctgcaac ctcaccattc
   106081 atggcgttca ggggcatgtt gcctacccac atctggctga caatccggta catcgcgcag
   106141 cacctttcct taatgaatta gtggctattg agtgggatca gggcaatgaa ttcttcccgg
   106201 cgaccagtat gcagattgcc aatattcagg cgggaacggg cagtaacaat gttattccgg
   106261 gtgaactgtt tgtgcagttt aacttccgct tcagcaccga actgactgat gagatgatca
   106321 aagcgcaggt acttgccctg cttgaaaaac atcaactgcg ctatacggtg gattggtggc
   106381 tttccgggca gccatttttg accgcgcgcg gtaaactggt ggatgcggtc gttaacgcgg
   106441 ttgagcacta taatgaaatt aaaccgcagc tactgaccac aggcggaacg tccgacgggc
   106501 gctttattgc tcgcatgggg gcgcaggtgg tggaactcgg gccggtcaat gccactattc
   106561 ataaaattaa tgaatgtgtg aacgctgccg acctgcagct acttgcccgt atgtatcaac
   106621 gtatcataga acagctcgtc gcgtgatgag tggttctgca agaggaaata agcatggact
   106681 ggctggctaa atattggtgg attctggtga ttgtcttttt ggtcggcgtc ctgctgaacg
   106741 tgattaaaga tctcaagcgc gtcgaccata agaaatttct cgccaacaag ccggagcttc
   106801 ccccgcatcg tgatttcaac gataagtggg acgatgacga cgactggccg aaaaaggatc
   106861 aaccgaagaa gtaagctgag tgctcaaccg taggccggat aaggcgttaa cgccgcatcc
   106921 ggcaatggtg aacgatgcct gatgcgacgc tcacgcgtct tatcaggcca ggtaatctgt
   106981 cttttacatc atttcaatca catcatcatc gccaggctta ccgccactta gcgcttggtc
   107041 gaaataatgt ttcggaatgg tataacgcaa gtgatccagg gcaaattgca tactgcgatt
   107101 gtcgatcgca tggcctaaat cctccacgat atccagcgtc acgtcaccac cggcactgat
   107161 taacgcttcc tgcgccgcaa ctgcatgcgc cagatcgata accggatctt caccaccatg
   107221 aatcaagtga attgtcgtgg ctgtagacgc ggtctccggc aggctcgcat agcgtccgtt
   107281 aaaggcaatg acgcgcgaag caaggcccgg ttcggcttta atgctctcca gcgccataat
   107341 cgcgccttgt gagaaaccaa ttagcgccgt ggcgttagcc cctactccgc tctgtttctg
   107401 ccagtagcgt accgtttcaa taaacgtcgg catgatggca tccacacgcg cctggcgatt
   107461 atcttccgta ataccctgca ctgaaaacca ctgacgcccc gccggattac cgctcggctc
   107521 cgcgccaccg acactcacca ccagcgcatc aggaaacagc ggtgcaaacc agctgcctat
   107581 ttcccccatt gccaccgggt tatccccgac accgtggaaa agcagtaaca actgttgtgc
   107641 aggtttatcc gggctttgaa caacaaaatg gtcatgtttc atggcgaact ccttaactga
   107701 tgtcattaat tttacgccgc tgactcacaa tgaccatggg agaaaattga aggagtcagt
   107761 gaaaaaattg ccattgcgtt atgcgatcgc gcagacgctc agtgcgaacc tcatttagtg
   107821 cgaacagcgc ctgcgcggcc tcttcccgct ggcgaaccag taacatcttg cggcctgaaa
   107881 gtttaagtgt ggtacataac tgcgcgtcac tggcgttttt ctgtaaacac ccacgcagcg
   107941 ccggtaatgc cagttcactg gtttgtagca gacgcaataa gcaacctaac gatgttaata
   108001 gcggacgatg agcgaaagca aaaccggcca gttcaagcca gtcgtcgtca gaaaggatgg
   108061 catcgtttag tggatcaaca ggaagcattt cgccattcca ctgcgcgaga gcttgcgcat
   108121 cgcgacgtaa acggtaatgc tcacgttcag ccagctgttt acccgcatca ctcatcggta
   108181 acagcgccat cgccgtatag caaccgctgc tggcttcccg atgattaccc atccgcacca
   108241 gcacaaaacc gcagcgttgc cagaaacgcc ataactcccc agtgtaacca aaactcaccg
   108301 aaagatagtc gaggtcatgc gtatattgca aagctccagc aataagttgc cgccctgtgc
   108361 cttcccgctg acgagccgga tgaactgcta tccggctaac ccgccttccg cgcaatgtcg
   108421 ccgccagtgg attgctgccg tgcgccgcca gcgactgggc caccagatta ccccgcgggc
   108481 gacgaaaacc tgcccatacc gcctgactga gttcttgaga taatccgccc tcatccacca
   108541 gccacaacgc cccggcaatc tcgttttcgc cagccgcctg taaaaaatgt tgccctagtg
   108601 catccatcat ccggcgtaaa tccagcggcg aggtccggta gtgcgcacca gacaataact
   108661 gataaacctt taacggcgtt tctggctcgc ttcgccataa cgtttgttca aatgcggaaa
   108721 tgacgatatt gccttgtggt gtatgggtga agttttcatc gttaaaaacc agtgcctcac
   108781 taaccatttt ttccagcggg catccctgtg cccagcggat cggctgttgc agttcaaaac
   108841 ggtgtaaatg cggaaagcga gcgcaaaatt tcagcaaaaa accacgtccg gtaccttcgt
   108901 agccctgcac cgtagtggtt aacaacgttc gaggaaaacg cgataccagt tgatgcaaca
   108961 acggcgcagg tatggctgcg gcttcgtcga ccaccagcca gtcggcttgc tcatcgctgg
   109021 ctaacaaggc atccggtgca ataaagcgaa acttctcgcc cgcaaattgt gccagtacat
   109081 ccgttgccgc ttttgcgggc gcggtgacaa tcgcactgcc cgcaatacga gaaatgagtt
   109141 gccctgccaa cgccgactta ccgcgcccac gcgcagccgt taccgctgcc acgcccggcg
   109201 gcatggtcag tagctgctgt aagagttgct gttgttctgg ttgtggtgcg ccggtcgcgg
   109261 ggtgccagtc agtacgggga gtaaaatgcg ccaacgagaa cggctggttt tgccgccaga
   109321 ggatagcgtc gttatccgcc gtaagtacgc gtttgagatg ctggacaaaa tgcggcgtcg
   109381 caatagggtc agggcaatca ctccagcgca gcgagtcggc atcaggttgg ttttcccact
   109441 cttcccatac agggagtaac aaaaccagcc agcttcccgc tttcaacgtt ccgctaagtg
   109501 ctgcaaaggc agcggcatca aagccgtggc gggcgtcgaa taccgcatgc cggaactcgc
   109561 gcccaagtaa agtttgtagc gccgagggtg aacagtggtt ttcagcatct agctgcggcg
   109621 aaatccacag ccagtcgcca ggtaaggcat cacgcaactt aagagcatga tcaaaacacc
   109681 aaccctcttc tccgctcaac accagcaagc ggcagatccc ttcacgtttc atttgcgctg
   109741 ttaatgtgta aagcgcagtc agttcagcca tccctgcccc ggaaattaaa tgcttttacc
   109801 aaaagtattg cattgtgccg gatcgccgct gtcgaaacca cgtttaaacc agctgcagcg
   109861 ttgctgagaa gtgccatgtg tgaaactgtc tggtactact cgcccctgac tttgctgttg
   109921 taaacggtca tcgccgatgg cctgcgccgc attcagcgcc tcttccagat cgccagtttc
   109981 cagaacgcct tgctgctgca tactatgccc ccagacaccg gcaaaacagt cggcctggag
   110041 ttccatacgc acagataagc ggtttacttc cgcctgcgtc gcgttttgtt gcagttgacg
   110101 aactttcggc tcgatgccta acagtttctg cacatgatga ccgacttcat gggcgataac
   110161 gtacccctgg gcaaaatcgc catccgcgcc aagtttgtct ttcatgtcat catagaagga
   110221 gagatcgata taaactgtgc catccgccgg gcaatagaac ggccccataa tggactggcc
   110281 cgccccgcag ccggtgcgcg tcattccacg gtacatgacc agcttcggtt gctgataggt
   110341 cttacccatc ttttcgaact gttgtcccca ggtgtcttcc gtggttgcca gaatcaccga
   110401 ggtgaatttt gcggcttcgt cttcattagg gctaattgac cgcgttgatt gttgttgaga
   110461 aaccggctgc ccggtcatca acccggttaa atcaacgcca tagtagcctg caaccagcac
   110521 gactatcagt aaaatcagcc cgcctttacc gctcggcaga cggaaaccgg gaccgcccat
   110581 tgacggaccg ccagagctgt tgcgcctgtc ttcaacattg tcactttcac gtcgcccttg
   110641 ccaacgcata gatacctcaa caatatattc attatagaaa tgatcgtagg tggttcagag
   110701 gaagattacc acaggaaagc gggatgaaat ggcggaggaa tgagcgttca atgcatatat
   110761 tccgagagcg gttgtcttgc cgtgccagct gcacggcaag atgatgaaca gaaaaatcag
   110821 tccagctgta cacccaggcg gcgggcgacg gcttcatagg cttcgatcag gccaccgagg
   110881 ctctggcgga aacggtcttt gtccattttc tccagtgttt ctttgtccca caggcggctg
   110941 ccgtccgggg agaactcatc gcccagtacc acttcacctt tgtacagacc aaactccagc
   111001 ttgaagtcga ccagaatcag accagcatca tcgaacagtt ttttcagcac gtcgttcgct
   111061 ttgtaggtca gctctttcat acgtgccagg ttctctttgc tcacccagcc aaaggtttcg
   111121 cagtaagatt cgttgaccat cggatcgtgc atggcatcgt ttttcaggaa cagatcgaac
   111181 agcggcgggt tcagctcaat accttcttcg attccaagac gtttcaccag agagccagca
   111241 gcacggttac gcacgacaca ctcaaccggc accatatcca gctttttcac cagacattcg
   111301 gtatcggaga gcagacgctc catttgagtc gggatacccg cttcagccag tttgctcata
   111361 atgaagtagt tgaacttgtt gttcaccata cctttgcgat caaactgctc aatgcgcgcg
   111421 ccatcccctg ctgacgtatc attgcggaat tcgagcacca acaggtccgg gttttccgtg
   111481 ctgtatacgg ttttcgcttt accacgatac aactcagctt gcttttgcat ctttatcact
   111541 cctgggtgtg aattaacgtt ttaaaatctt ttgctgtctg gtgtgccgga tgttttttcg
   111601 gatgcggcgt gaacgcctta tccgaactac gtccgacaaa caaatttcgt gcgaattacc
   111661 gctaaaatcg cgttttcctg ccgacgcaca cgtttgcgta tcatatcaga aaaaagggcc
   111721 ggatgattcc agccctgtat ttttacttgc taaacgcagc ctggaagaca gctaccagcg
   111781 cgtcgttctg actctgagtc agagtatgac ctttcggatc gatgaactgt aggctgctgc
   111841 ggttatctaa atcgccaacc tgcagtttat agtcaccgga tgccaggcct ggatcgctcg
   111901 cgcccagttc ctgccagtcg ctgtcagaca gcggcttata agttacggcc atgttgccct
   111961 gcgaacgggt gctgtcggtc actttcatgc ccactttttc cagcgacgct ggcagacgtt
   112021 gccaaaccac attgaacggc ccgcgtacga ccagcattgg taaaccggtg tcatcagctg
   112081 cactttgtac gtccatagtg gtggaggcac gattttgcgc agcgttcgcg gcgtcagtgg
   112141 cagatttatc cagaccggcg gaaataacgt tcatcatctc cgtgctgtaa cgctgcatgg
   112201 aagccgcgtc tgcaaccggt ttgcccgcct gttccaggtt cagcagttta accgtaaccg
   112261 cctgctgata accctgcggc ttaacagaga tttgataacg accacgatac tgctcgtctt
   112321 cgtccagacg gttccattgt acccaatcgg tggtcagtgt ctgaccagca tcatcacgtt
   112381 gggtgatggt gtagtttttc gcctgcagca cgctaaccac ctgcggccac agagtattgc
   112441 cacgaccatt ttccaccagc aatgaagcgg tatcgcccgt gaactgggta cgcgcgccag
   112501 aaaccagtgc cagcggctgg gctggtggac gaatgtccag cgccttaccg acagcaccac
   112561 taccgttggt caccgggatt gcataatcac cggaggtcac cggcaaaatc attccagccg
   112621 gggcatgaag ctccgcaagc ggtgccgctt ccaggtaggc ttcatcacca ctgacctgac
   112681 gcttatagcg tgagtcagaa ctacaggcag cgagtaataa aacaagcgaa acacccgcaa
   112741 cctttgccag gcgcgacttt tgaacagagt aagccatcaa atctccctaa actttacagc
   112801 aaaccggcat gcttaagcgc cgctctgacc gtctcacgac cactgtcggt gattggtgtc
   112861 attggcaggc gcagcgtatc ggtcgccaca agacccagtt ccttacatgc ccatttcacc
   112921 gggattggat tgggttcgac aaatagtttg ttgtgtaatg gcatcagacg ctgattaata
   112981 acgcgtgcct cggcaaaatg cccttctgct gccagtttgc acatctgggc catatcacgc
   113041 gctgcgacgt tagccgtaac ggaaataacc ccatgaccgc ccaattgcat gaagtccagc
   113101 gcgctcgcat catcgccgct cagcagaaca aaatcatctg aaaccagctc tttgatctgg
   113161 tttacacgcg ttaagttccc tgttgcctct ttgattccga taatattttt tactttcgcc
   113221 agacggccca ccgtttccgg gagcagatcg cagccagtac gggacggcac attatacaga
   113281 atttgcggca ggtcagtatg ctcagcgatg gctttgaaat gctgatacaa accttcttgc
   113341 gacggacgat tgtagtaagg ggttaccgtc aggcagccga cgataccact gtcattgaag
   113401 cgctgcgtca ggctaatggc ttccgcagta gcgttagcgc cggtcccggc aattaccgga
   113461 atgcgcccat cagccagatc cagcgtcatc atcaccacat cagcatgttc gtcatgattt
   113521 aaggtagcgg actcgccagt ggtgccaaca gaaacgatcg ccgaagtacc gctggcgaca
   113581 tgataatcaa tcagtttttt caagctagcc cgacagacat tacctttttc atccatcgga
   113641 gtaacaatcg cgacaatact tcccgtgaac atgggccatc ctctgtgcaa acaagtgcct
   113701 caatggtacg tttggtatgg cattaaaagc aagcagacag aaccgttctg attgttgtat
   113761 gcatgatttt tttatgcttt ccttaagaac aactcacccc ttaaaggaat aaccagtttg
   113821 acactgtcat cgcaacatta tctggtgatc actgcgttgg gtgccgatcg ccctggaatt
   113881 gtgaacacca tcacccgtca tgtcagtagt tgcggctgta atattgaaga cagtcgcctg
   113941 gcgatgctgg gagaagagtt cacgtttatc atgctgcttt ccggttcatg gaatgccatc
   114001 actctgattg aatcaacgtt accgttgaaa ggtgccgaac tggatctttt aatcgtgatg
   114061 aagcgcacga cggcgcgtcc gcgtccgcca atgccagcat ctgtctgggt tcaggtcgat
   114121 gttgcagact ccccgcattt aattgaacgc ttcacagcac ttttcgacgc gcatcatatg
   114181 aacattgcgg agctggtgtc gcgcacacaa cctgctgaaa atgaacgggc tgcgcagttg
   114241 catattcaga taaccgccca cagccccgca tctgcggacg cagcaaatat tgagcaagcg
   114301 ttcaaagccc tatgtacaga actcaatgca caaggcagta ttaacgtcgt caattattcc
   114361 caacatgatg aacaggatgg agttaagtaa tgaatccact gaaagccggt gatatcgcac
   114421 cgaaatttag cttgccggat caagacggag aacaagttaa tttgaccgac ttccagggac
   114481 agcgtgttct ggtttatttc tacccgaaag ccatgacccc cggctgtacc gtacaggcct
   114541 gcggcttacg cgataacatg gatgagttga aaaaagcggg cgttgatgtg ctgggtatca
   114601 gcaccgataa acccgaaaaa ctctcccgtt ttgcggaaaa agagctgctt aactttacgc
   114661 tcctgtctga tgaggaccac caggtgtgcg aacaattcgg cgtctggggt gaaaagtcct
   114721 tcatgggcaa aacctacgat ggtattcatc gcatcagctt cctgattgac gctgatggca
   114781 aaatcgaaca tgtctttgac gatttcaaaa ccagcaatca ccacgacgtt gtgctgaact
   114841 ggctgaaaga acacgcctga ttactttgct ccattccgtg ctggctgcgc ttgcggccag
   114901 catacctcac ttctcgtgat caagatcaca ttctcgcttt cccctgcgac acgggtgtcg
   114961 aatccatttt ttgctgaacg ttaatgacca tcatttttgt accgttcaga atccagttaa
   115021 tacataactt attgaataga ttgagttaat cagaatggca ccctttatgc aatatgaaat
   115081 gcaatgtttc atatcatttt caaggagccg acatgaaccg ctttgtggtg gccgaaccac
   115141 tgtggtgtac aggatgtaat acctgtctcg ctgcctgttc ggacgtgcat aaaacgcaag
   115201 gtttacagca acacccgcgc ctggccctgg cgaagacgtc aacaatcact gcccctgtcg
   115261 tgtgtcatca ctgtgaggaa gccccttgcc tgcaggtctg cccggtcaat gccatctctc
   115321 agagggatga tgcgatccaa ctcaacgaaa gcctctgtat tggctgcaag ctttgcgccg
   115381 tggtctgccc atttggcgca atcagcgctt caggaagccg tccggtgaat gcccatgcgc
   115441 aatatgtttt tcaggctgaa ggctcactca aagacggcga agaaaacgtg ccgccccaac
   115501 atgcgttgct gcgctgggaa ccgggtgtcc agaccgtcgc ggtgaaatgc gacctgtgtg
   115561 atttcttgcc agaaggtccg gcctgcgttc gcgcttgccc gaatcaggcg ttacggctga
   115621 tcaccgacga tagcctgcaa cgtcagatga aagaaaaaca gcgccttgcc gcaagctggt
   115681 ttgccaatgg cggggaggat cccctttccc tcactcagga gcaacactaa tggatgccct
   115741 gcaattatta acctggtcgc tgatcctcta tctgtttgcc agtctggctt cgctgttttt
   115801 actcggtctg gacagactgg ctattaagct ttccggcatc acatcgctgg tgggcggcgt
   115861 gattggcatc atcagcggaa ttacgcaatt acatgctggt gtaactttag tcgcccattt
   115921 tgccacccct tttgactttg ccgatttaac cctgcgaatg gatagcctct cggcatttat
   115981 ggtgctggtt atctccttgc tggtggtggt ttgttcgctc tattcattga cttatatgcg
   116041 cgaatacgag ggcaaaggcg cggcggcgat gggcttcttt atgaatcttt tcatcgcatc
   116101 gatggttgcc ctgctggtga tggacaacgc tttttggttc atcgtgctgt ttgaaatgat
   116161 gtcgctgtct tcctggtttc tggtcattgc caggcaggat aaaacgtcga tcaacgctgg
   116221 catgctctac ttttttatcg cccacgccgg atcggtgctg attatgatcg ccttcttgct
   116281 gatggggcgc gaaagcggca gcctcgattt tgccagtttc gcacgctttc actttctccg
   116341 gggctggcgt cggcggtgtt tctgctggcc tttttcggtt ttggcgcgaa agccgggatg
   116401 atgccgttgc acagctggtt gccgcgcgct caccctgccg caccatcgca cgcttcggcg
   116461 ttgatgtctg gcgtaatggt caaaatcggt attttcggca tcctgaaagt agcgatggat
   116521 ctgctggcgc aaacgggttt gcctctgtgg tggggcattc tggtgatggc gatcggcgca
   116581 atctccgcgc tcctgggcgt gctatatgcg ctggcggaac aggatatcaa acggctgctg
   116641 gcctggagta ccgtcgaaaa cgtcggcatt attttgctgg cagtcggtgt ggcgatggac
   116701 ggtctgtcac tgcacgaccc gctgctcacc gtggttggac tgctcggcgc actgtttcat
   116761 ctgctcaacc atgcgctgtt caaagggctg ctatttctcg gcgcggacgc gattatttcg
   116821 cgtttgcata cccacgacat ggaaaaaatg ggggcactgg cgaaacggat gccgtggaca
   116881 gccgcagcat gcctgattgg ttgcctcgcg atatcagcca ttcctccgct gaatggtttt
   116941 atcagcgaat ggtacacctg gcagtcgctg ttctcactaa gtcgtgtgga agccgtagcg
   117001 ctacaacttg cgggtcctat tgctatggta atgctggcag tcactggtgg gctggcagta
   117061 atgtgcttcg tcaaaatgta cggtattact ttctgtggtg cgccgcgcag tacacacgct
   117121 gaagaggcac aggaagtgcc aaatacgatg atcgtcgcca tgctactgct cgcggcactc
   117181 tgcgtattaa ttgcgcttag tgccagttgg ctggcaccga agataatgca tattgcccat
   117241 gcgtttacca atacccctcc cgtcactgtc gccagcggaa tagcacttgt acccggcacg
   117301 tttcatacac gggtcactcc ttcattactg ttgctgttac tactggcgat gcctttgctg
   117361 ccaggccttt actggctgtg gtgtcgttcg cgccgcgcag cgtttcgtcg cacaggagat
   117421 gcctgggcat gcggctacgg ctgggaaaac gcgatggctc cgtcaggcaa tggcgtgatg
   117481 cagccgctgc gtgtggtctt ctctgcgtta tttcgtctac gacaacagct cgaccctacg
   117541 ctgaggctaa ataaaggtct tgcgcacgtc accgccaggg ctcagagcac agaacccttc
   117601 tgggatgagc gggtgatccg ccccatcgtg agcgccaccc aacggctggt caaagaaata
   117661 cagcatctgc aaagcggcga ctttcgtctc tattgcctgt atgtggtcgc cgcactggtt
   117721 gtgctgctaa tcgctattgc cgtctaagga aatcaccatg agacaaactc tttgcgacgg
   117781 atatctggtc atttttgcgt tagcacaggc cgtgattctg ctgatgctaa ccccactttt
   117841 tacgggtatt tcccggcaga tacgcgcgcg tatgcactcc cgccgcgggc cggggatctg
   117901 gcaggattat cgcgatatcc acaaactgtt taaacgccag gaagttgcgc cgacatcttc
   117961 aggtctgatg ttccgcctga tgccgtgggt attaatcagc agcatgctgg tgctggcgat
   118021 ggccttacca ctatttatta ccgtttcccc ttttgcgggc ggcggcgatc tgatcaccct
   118081 tatctatctt cttgccctgt ttcgtttttt ctttgctctt tccgggctgg ataccggaag
   118141 tccgtttgcg ggagtcggtg ccagtcgcga gttgacgctc ggcattctgg tcgaaccaat
   118201 gcttattctc tcactgctgg tattggcgct gatagcaggt tccacgcata tcgagatgat
   118261 cagcaatacg ctggcgatgg gctggaactc gccgctaacc accgtactgg cgttactggc
   118321 ctgtggtttt gcctgcttca ttgagatggg aaaaattccc tttgatgttg ctgaagcaga
   118381 acaggaatta caggaaggcc cgctgaccga atattccggt gccgggctgg cgctagcgaa
   118441 atgggggcag gggctgaaac aggtcgtgat ggcagcactg tttgtggccc tgtttctgcc
   118501 ctttgggcgc gcgcaagaac tttctctcgc ctgcctgctg acttcacttg tcgttacgct
   118561 gctcaaggtt ttgctgattt ttgtactggc ctcaatcgca gaaaacacgc tggcacgcgg
   118621 gcgtttttta ctcattcacc atgtgacctg gcttggcttg gcttcagcct tgctgcgctt
   118681 gcatgggtct tctggttaac cggtctgtaa ggagcactga cggaatatgg aaaatcttgc
   118741 tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt cgccacaacg
   118801 tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca tgttgtcgct
   118861 gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg tcaacgtggg
   118921 ggatgtggca ttgtttggcc tggtcattga tcgcgtgagt acgctgattc tgtttgtggt
   118981 ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg ataaaaatcg
   119041 cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt ttatcggcgc
   119101 gatggcggga ctggtactct cctcgacgct gctcggtcag ttgttgtttt ttgaaattac
   119161 gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc agcgttcagc
   119221 actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg ccgccgccac
   119281 gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac atggcgacgc
   119341 acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg cccagctacc
   119401 gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg actatctcca
   119461 cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta tcgacggcgg
   119521 caatatcccg catgtgattg gcggcgttgg catggtcatg gcactggtca ccattcttta
   119581 tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct ggtcgaccat
   119641 cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct cgcggctggc
   119701 gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc tgtttttcct
   119761 tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc tgcgtggcgt
   119821 attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg caattaccgg
   119881 tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg gttttgcgtt
   119941 gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg aatcggtcgc
   120001 cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac cgagcgaggc
   120061 cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg tactgattgt
   120121 gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag atgatgaatg
   120181 accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc gctgtttgtg
   120241 attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc gctggtgctg
   120301 gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct gatctggtcc
   120361 gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta cgctgcacga
   120421 aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat ggcactgctc
   120481 gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct accgatggct
   120541 accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg cctgctgtgc
   120601 attgtcagcc agcgcaatat cctgcggcaa atttttggtt actgcctgat ggaaaacggc
   120661 tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga aataggtatc
   120721 gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa aatatggcgt
   120781 acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg agatgagtta
   120841 ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc tctgttttgc
   120901 ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata gcttagggat
   120961 cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg caggagaaat
   121021 attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc tcgccattct
   121081 tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc atgaagtggc
   121141 acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc atctgttttt
   121201 tttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg cggcgatcga
   121261 agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt catcgctgga
   121321 atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc tgttcggtac
   121381 cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg cgatattctg
   121441 gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt tggcctttgt
   121501 gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg cctggctgcc
   121561 ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg tattgctgaa
   121621 ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca tcggcagcga
   121681 tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg cggcattttt
   121741 cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg agaacatggg
   121801 gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg cgctgctgca
   121861 caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca atgtactgct
   121921 caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca tgccatttac
   121981 cgccgtgctg tttggcggcg gtgcgctggc gctggcaggg atgccgccct tcaacatttt
   122041 tcttagcgaa tttatgaccg ttaccgcagg actggcacgt aatcacctgc tgattatcgt
   122101 cctgctgtta ttgctgttaa cgctggtgct cgcgggcctg gtacggatgg ctgcgcgggt
   122161 gttaatggcg aaaccgccgc aggccgttaa ccggggtgat ctcggctggt tgaccacctc
   122221 gccaatggtg attctgctag tcatgatgct ggcgatggga acgcatattc cacaacctgt
   122281 catcaggatc ctggcgggcg cttccactat agtcctctca gggacgcacg acctgcctgc
   122341 acaacgtagc acctggcatg attttttgcc ttcaggcacc gcatctgttt cggagaaaca
   122401 cagtgaacgt taattcatcg tcaaatcgtg gcgaagcgat tctcgccgcc ctgaaaacgc
   122461 agttccccgg cgcggtgctg gatgaagagc gacaaacgcc tgaacaggtc accattacgg
   122521 tgaaaatcaa tctgctgcct gacgttgtac attatcttta ttatcaacat gatggctggc
   122581 ttccagtcct gtttggcaac gacgagcgga cacttaacgg tcattacgcg gtttattatg
   122641 ccctttcaat ggaaggggcc gaaaaatgct ggatcgtggt gaaggcactg gtcgatgccg
   122701 acagtcggga gtttccgtca gtcacaccgc gcgtccctgc cgcggtctgg ggcgagcgag
   122761 aaattcgcga tatgtacggg ctgattccgg ttggcctgcc ggatcagcgt cgcctggtgt
   122821 tgcccgatga ctggccggaa gatatgcatc cgctgcgcaa agatgcgatg gattatcgac
   122881 tgcgccctga accgacgaca gattccgaaa cgtatgcgtt tatcaatgag ggcaacagcg
   122941 atgcgcgggt gatccctgtc ggcccgctgc atatcacctc ctatgaaccg ggtcacttcc
   123001 gcttgtttgt ggatggcgag caaattgtcg atgctgatta ccgcctgttt tatgtccatc
   123061 gcggcatgga gaaactggca gaaacgcgga tgggctacaa cgaagtgacc ttcttatcgg
   123121 accgcgtgtg tgggatttgc ggttttgccc acagtgtggc ctataccaac tcggttgaaa
   123181 atgcactggg gattgaggtg ccgcaacgag cacatactat tcgctcgatt ctgctggaag
   123241 tcgagcggct acacagtcat ttgctcaacc ttggcctctc ctgccatttt gttggttttg
   123301 ataccggctt tatgcaattt ttccgcgtgc gggaaaagtc gatgacgatg gcggaattgc
   123361 tgaccgggtc gcgtaaaacc tacggtctga atctgattgg tggtgttcgc cgcgatattc
   123421 tcaaagagca acgtctgcaa acgctgaaac tggtgcgcga gatgcgctcc gacgtgtcgg
   123481 agctggtaga gatgctgctt gctacgccga atatggaaca acgcactcag ggcattggca
   123541 ttctcgaccg acaaatcgcc cgtgattata gccctgtagg gccgctgatc cgcgcagtgg
   123601 ttttgcccgt gatttgcgct ttgatcaccc ctacgccgac tacggcaata ttccaaaaac
   123661 actgtttacc tttaccggcg gcgatgtttt ctcccgcgtg atggtccgtg tcaaagagac
   123721 gtttgattcg ctggcaatgc tggaatttgc cctcgacaac atgccggata ccccactgct
   123781 gaccgaaggc tttagctata aacctcacgc attcgcgctg ggctttgttg aagcgccacg
   123841 cggtgaagac gtgcactgga gcatgctcgg tgataaccaa aaattgttcc gctggcgctg
   123901 tcgtgccgcc acctacgcca actggccggt gttgcgttac atgctgcgcg gcaataccgt
   123961 ttctgacgca ccgctgatta tcggtagcct tgatccctgc tactcctgta ccgaccgtgt
   124021 gacgctggta gatgtgcgca agcgccagtc aaaaaccgtg ctgtataaag agatcgaacg
   124081 ctacggcatt gatcgtaacc gttcgccgct gaagtaagga cagaagatgc tgaagttact
   124141 gaaaactatt atgcgcgccg gaaccccgac ggtgaaatat cccttcgcgc cactggaggt
   124201 cagcccaggc tttcgcggga aaccggacct gatgcccagc caatgtattg cctgcggtgc
   124261 ctgcgcctgt gcttgtccgg caaatgcgct gactatccag accgacgacc agcaaaattc
   124321 gcgcacctgg cagctctatc tggggcgttg tatttactgc ggacgttgtg aagaagtgtg
   124381 cccgaccaga gccatccagc ttaccaataa ctttgaactg accgtcaccc ataaagccga
   124441 tctctatacc cacgcgacgt tccatctaca acgttgcagc cgttgcgaac gcccgtttgc
   124501 cccgcaaaaa accgtcgcac tggctgctga attgttggca cagcaacaaa atgctccaca
   124561 aaaccgcgaa atgttatggg cgcaagcgag cgtctgcccg gagtgcaaac aacgcgcgac
   124621 gctgatcaac gacgatacag atgtaccgct ggtggctaag gagcagctat gagtccagtg
   124681 cttacgcaat atgtcagcca gcccatcacg ctggacgagc aaacgcaaaa gatgaagcgg
   124741 catttgctac aggatatccg tcgctcggct tacgtttatc gcatcgattg cggcggctgc
   124801 aacgcctgtg aaatcgaaat ttttgctgcc atcacccccg tattcgacgc agaacgtttt
   124861 ggcattaagg ttgtttcatc accgcgtcac gccgatattt tgttatttac tggcgcagtc
   124921 acccgggcga tgcgtatgcc tgcacttcgg gcgtatgagt ctgcccccga tcataaaatc
   124981 tgtgtttcct acggcgcgtg cggtgtcggc ggcggtattt tccacgatct ctacagcgtc
   125041 tggggcggca gcgacaccat tgtccccatt gatgtttgga tccccggctg cccgccaaca
   125101 ccggccgcca ccattcacgg tttcgccgtg gcgctcggtt tgctgcaaca gaagattcac
   125161 gcggtggatt atcgcgatcc caccggggtg actatgcaac cgttgtggcc gcagatcccg
   125221 ccatcacagc gtatcgccat tgagcgagaa gcgcggcggc tggcgggcta tcgtcagggg
   125281 cgagaaattt gcgatcggct cctgcgccat ttaagcgacg atcctacagg aaatcgggtt
   125341 aacacctggt tgcgcgatgc cgacgatcca cgtctcaata gtatcgttca gcaactcttt
   125401 cgcgtactcc gggggttaca tgactgaaga gtgcggggaa attgttttct ggacgctgcg
   125461 aaaaaagttt gtcgccagta gcgacgagat gccggaacac agctctcagg taatgtatta
   125521 ctcgctggct atcggtcatc acgttggcgt gattgattgt ctgaatgtcg ccttccgctg
   125581 cccactgacg gaatacgaag attggcttgc actggtcgaa gaggagcaag cccgacgtaa
   125641 gatgctgggg gtgatgactt ttggtgagat tgttattgac gccagccaca ccgccctgtt
   125701 gacccgggca ttcgcgccac tggcagatga cgcgacgtct gtgtggcaag cgcgtagcat
   125761 tcaattcatt catctgttgg atgaaattgt gctggaaccg gctatctatc tgatggccag
   125821 aaaaattgcg tgagaaggat ttctcattaa taaggactgt tgatggctat gtcagacgaa
   125881 gcgatgtttg ccccgccgca aggaataaca attgaagcgg taaacggaat gctcgcggag
   125941 cggttagcgc agaaacacgg caaggcgtct ttattacgcg ccttcatccc gctgccgccg
   126001 ccgttcagcc cggtacaact tattgaactg catgttctca aaagcaactt ctattaccgc
   126061 taccatgatg atggcagcga tgtgacggca acaacagagt atcagggcga gatggtcgat
   126121 tattcgcgtc acgccgtcct tctcggcagt agttgaatgg cggagctacg ctttattcgt
   126181 acccacggca gccgttttac tccccaggat tgcacactgt ttaactggct ggcacgcatt
   126241 atcaccccgg ttctgcaatc atggctcaat gatgaagaac agcaggtggc gctgcatttg
   126301 ctggagaaag atcgcgatca tcatcgggta ctggtggata tcactaatgc agtgctgtca
   126361 catcttgatc tcgacgatct gatcgctgac gtcgctcgtg agatccatca ttttttcggt
   126421 ctggcttcag tcagtatggt actgggcgat catcgaaaga acgagaagtt cagcctgtgg
   126481 tgcagcgatc tttctgcctc acattgtgcg tgtctgccac acaatatgcc tggcgacagt
   126541 gtattgctga cacaaacgct acaaacccga caaccgacct tgacgcaccg tgcagatgat
   126601 ctgtttctct ggcaacgcga cccgttatta ctcttacttg catctaacgg ctgcgaatct
   126661 gcgctcctta taccgcttac ctttggcaac catacaccgg gtgcattgtt gctggcgcat
   126721 acctcttcca ctctctttag tgaggaaaac tgccagctac tacaacacat agccgatcgc
   126781 atcgctattg ccgttggcaa tgccgatgcc tgccgtagaa tgaccgattt gcaggaaagt
   126841 ttgcagcaag aaaaccacca gcttagcgag cagctccttt cgaatctggg cgtcggtgac
   126901 attatctatc aaagccaggc aatggaagac ctgctccagc aggtagatat tgtggcgaag
   126961 agcgacagta cggtgttgat ttgcggtgaa accggaaccg gcaaagaggt gatcgccaga
   127021 gcgatccatc aacttagccc gcgacacgac aagccgctgg tcaaaatcaa ctgcgctgcc
   127081 atccccgcca gtcttctgga aagtgagtta ttcggtcatg acaaaggggc gtttactggt
   127141 gcgattaata cccatcgtgg tcgttttgaa attgccgatg gcggcacgtt gtttctcgat
   127201 gaaattggcg atctgccgtt agaacttcag cctaaactgc tgcgcgtatt gcaggaacgg
   127261 gagattgagc gtctcggcgg gagtagaacg atcccggtaa atgtcagagt cattgccgcc
   127321 accaaccgtg atttgtggca aatggttgaa gatcgccagt ttcgcagcga tctcttttat
   127381 cgcctgaatg tcttcccact ggaattgcct ccgctgcgcg accgtccgga agatatccct
   127441 cttttagcaa agcatttcac gcaaaaaatg gcgcgccata tgaatcgttc aattgacgcc
   127501 atcccgaccg aggcgctacg ccagttgatg tcgtgggact ggccgggcaa cgtgcgcgag
   127561 ctggaaaacg tgattgagcg agcggtactg ctgactcgcg gtaacagtct gaatttacat
   127621 cttaatgtcc gacaaagccg tttactgccg acgctaaatg aagattcagc gcttcgcagt
   127681 tcaatggcgc agttgctgca cccgacgacg ccagagaatg acgaagaaga acgtcagcgc
   127741 attgttcagg tattgcgaga aaccaatggc attgttgccg ggccccgtgg cgcggcgaca
   127801 cgattaggga tgaagcgcac cacgctgctg tcacgaatgc agcgtctggg aatctcggtt
   127861 cgcgaggtgt tgtaatctgc ttttgcagga gtatgcatga gaaacaaact ctctttcgac
   127921 ttgcagttga gcgccagaaa agcggcaatc gctgaacgga ttgccgccca taaaattgcc
   127981 cgcagtaaag tgtcggtctt tttaatggcg atgtccgctg gcgtgtttat ggcgatcgga
   128041 tttacttttt acctttccgt tatcgccgat gccccgtctt cataggcatt aacccatctg
   128101 gtgggcggcc tttgctttac acttggcttt attttgctgg cggtttgcgg caccagcctg
   128161 ttcacctcgt cggtaatgac ggtaatggca aaaagtcggg gcgttattag ttggcgaact
   128221 tggctgatta acgcacttct ggtggcctgc ggtaatctgg caggtattgc ctgtttcagt
   128281 ttgttaatct ggttttccgg gctggtgatg agtgaaaacg cgatgtgggg agtcgcgatt
   128341 ttacactgcg ccgagggcaa aatgcatcat acatttactg aatctgtcag cctcggcatt
   128401 atgtgcaatc tgatggtttg cctggcgctg tggatgagtt attgcggacg ttcgttatgc
   128461 gacaaaatcg tcgccatgat tttgtccatc accctgtttg tcgccagtgg ctttgagcac
   128521 tgtatcgcca atttgtttgt gattccgttc gccattgcca ttcgccattt cgcccctacc
   128581 tccttctggc aactggcgca cagtagcgca gacaattttc cggcactgac ggtcagccat
   128641 tttattaccg ccaatctgct cccggtgatg ctgggtaata ttatcggcgg tgcagtgctg
   128701 gtgagtattt gttatcgggc tatttattta cgtcaggaat cctgaaaaat cagcccggcg
   128761 aaacagttcg tcgggctgaa gattattctt gcgcgatttg cccatcgggc caggcgtgga
   128821 tcacggcttt gatcagcgtc gccaatggaa tggcgaagaa tacgccccag aatccccaca
   128881 aaccaccgaa gatcaccacc gataaaataa tcaccagcgg atgcaggtta accgcttcgg
   128941 agaacaacac cggtactagc aggttgccgt ccagcgcctg aataatcaga tacaccgcga
   129001 agcagctcca gaattcagta cctgcgccaa actggaatag cgccacgcca accaccggaa
   129061 tggtcaccac aaatgcgcca atgtacggaa taagaaccga gaagccgacc agcactgcca
   129121 gcagcagcga atagttcagc ccgaagagca agaaccccag ccaggtggcg atccccacca
   129181 cgatcatctc cagcactttg ccgcggatat agttggtgat ttgttgattc atctccttcc
   129241 acacctgtcc tgccagccca cggttacgcg gcagcacccg gcgaacggcg ttcagcatct
   129301 gctctttgtc tttcagcagg aagaagacca tcaatggcac cagcaccaga tagacggcta
   129361 tggtcagcaa accgaccagc gaggcgaggg aaattttcac caccgaatcg cccatggtca
   129421 gcatccgact gcgcatattt tcggccattg catcaataat gcccgcgtcc attaacaccg
   129481 gatagcggcg cggcaacgtg gcggcaaagt cagaaagctt attgagcatc cccggcatat
   129541 cgcggattaa gtagatgccc tgttgccagg cgataggcag taccacgaac gccatcagta
   129601 gcaatatacc gacaaaaacc accaatacaa tcgacgtcgc ccagcggcgg gagcagccaa
   129661 tagattgcag gcgcacggtt ggccattcca gcaaataagc cagcacaata gccaccagca
   129721 acggagcaag caggccacta aagaaaaaga taatgccaaa tccggcaact aaaataacca
   129781 gcaaggcaat cgcttccggg tcgctaaaac ggcggcgata ccattgcatc aacatttcga
   129841 gcataaagac cttccctgaa cctcaagagc gggattgcga tccgcaattg tatcgaaatg
   129901 tcacaaaaaa gacttcgctt tttatgacgg attcaggaaa ctgaaaagtc atttgagtgg
   129961 gctaatcttc gccgttacac tcaaaggcgg cgaggtggga acgatatttc acagtatcgg
   130021 tcaaatgact atcttcagaa atacaggata gaggttatgt tcaggcagtt gaaaaaaaac
   130081 ctggttgcaa ccctcattgc tgctatgacc attggtcagg tagccccggc gtttgccgac
   130141 agcgcagaca ccttgccgga tatgggaacc tccgcaggaa gcacgctttc cattggtcag
   130201 gaaatgcaga tgggcgacta ttatgtccgc cagctacgcg gcagcgcgcc gttaattaat
   130261 gacccgctgt taacgcaata tattaattcg ctggggatgc gtctggtttc gcatgccaat
   130321 tcggttaaga caccgtttca tttttttctg atcaacaacg acgaaattaa cgcctttgct
   130381 ttctttggcg gcaacgtggt gctgcactct gccctgttcc gttattccga taacgaaagt
   130441 caactggctt cagttatggc gcacgaaatc tcccacgtca cccaacgtca cctggcgcga
   130501 gcgatggaag atcaacaacg cagcgcgccg ctgacctggg tcggcgcgtt aggttctatt
   130561 ttactggcga tggccagtcc gcaggcgggg atggcggcgc tgaccggtac actggcggga
   130621 acgcgccagg ggatgatcag tttcacccag caaaatgaac aggaagcgga ccgcattggt
   130681 attcaggtgc tgcaacgctc gggattcgat ccacaggcga tgccgacctt cctggaaaaa
   130741 ttgctcgatc aggcgcgtta ctcctcgcgc ccgccggaaa ttttattgac tcacccgttg
   130801 ccggaaagtc gtctggcaga tgcccgcaac cgtgctaatc agatgcgccc gatggtggtg
   130861 cagtcgtcgg aagatttcta tctggcgaaa gcgcgcacac tggggatgta taattccgga
   130921 cgtaaccagc tcaccagtaa tttgctggat gaatgggcga aaggaaacgt tcttcagcaa
   130981 cgagcggcgc aatatggtcg tgctttacag gcgatgaaag ccaataaata cgacgaggcg
   131041 cgtaaaacgc tgcaaccgtt actggcggca gaacctggca acgcatggta tctcgatctg
   131101 gctaccgata tcgatcttga gcaaaacaaa gccaatgagg caatcaatcg cctgaaaaat
   131161 gcccgcgatt tacgcaccaa tccggtgttg cagctcaacc tggcgaacgc ttatctgcaa
   131221 ggcggtcaac cacaagaagc ggccaatatt ctgaatcgct acacctttaa taataaagat
   131281 gacagcaacg gctgggattt gctggcacag gcggaagccg cgctaaataa ccgcgatcag
   131341 gagctggctg cgcgagcaga aggttatgcg ctcgccggac gactcgatca ggccatttcg
   131401 ctgttgagta gcgccagttc gcaggtgaaa ttaggcagcc tgcaacaagc gcgttacgat
   131461 gcgcgcatcg accagttgcg ccagctgcag gaacgcttta agccttatac caagatgtaa
   131521 ttcgcattaa aggaagaaca atgaccaaac aggtaaaaat ctaccataac ccacgctgtt
   131581 caaagagccg ggaaacgctg aacctgctga aagaaaacgg cgtggagcca gaagtggtgc
   131641 tctaccttga gacacccgcc gatgcggcaa cgctgcgcga tttgctgaaa atgctgggga
   131701 tgaatagcgc ccgtgaattg atgcgccaga aagaggatct ttataaagaa ctaaacctgg
   131761 ctgacagttc gcttagcgaa gaggcgctga ttcaggcaat ggtggataat ccgaagctga
   131821 tggagcgccc gattatggtc gcgaatggca aagcgcggat tggtcgtcca ccggagcagg
   131881 tactggaaat cgtgggttaa gatgtgctcc atcgctagtt gaagcacatt gccggatgcg
   131941 acgcttgcca cgtcttatcc ggcctacgaa tactatggat taacttcgcg taggtcaggc
   132001 gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
   132061 ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
   132121 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
   132181 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
   132241 agtggctgca atttgtagat ctgcccccag tcgagacgcg acgcgagatc cggtaatccc
   132301 agattcaact gccgcggtgg acgatcgccg gtgatcaaca accgtgtttt gcccgattcc
   132361 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
   132421 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
   132481 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
   132541 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
   132601 taaccgctat gttcctgacg cagcacgttt tgcagcgcgg ccagtaaaga ggagttatcc
   132661 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
   132721 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
   132781 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
   132841 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac tgatcagttt
   132901 gaaaatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt ttaactccgc
   132961 cgcgccgatg tttaccttcg cgccactgac gccgatgatc aaaatcacgg aagtcaggat
   133021 caggttctgt gctttgttgt aatccacttt cgattcgatc aaaacacgaa taccggaagc
   133081 accaatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
   133141 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccgcc
   133201 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
   133261 ggtatttggc gtagagccaa agaagccgga gatcacggtc gacaggccgt tagcaaacat
   133321 cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
   133381 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
   133441 aaaccactcg aagcgcggcg tatagagggt tggcagtgca aaccagtgag cattaataat
   133501 cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
   133561 taaaatcggg ataattgcca ggaaaccacg aaacagcacg gaacctaaaa ccgtgaccgc
   133621 cagggtggta atagagatga tgatggtttt ggagtttggc gtttgccctt cagccgggag
   133681 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
   133741 gcccattgcc gcaggtggaa acagcacgtc caaccagccg gtccccgctt tcttcacgat
   133801 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
   133861 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
   133921 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
   133981 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
   134041 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
   134101 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa
   134161 aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc gtaccaaaga
   134221 ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc agtccctggt
   134281 caatcgatgc agtatacagt tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt
   134341 ctggcgcagc gaccagcacc agaactttga tgctgctgca gcccgctttt ttcagcaggt
   134401 cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc agcgccatac
   134461 gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc gtttcttcat
   134521 tacggtacat accgacaacg ctgatgcgcg cgctcggaac gttttccagc acaccgtcca
   134581 tcataccaag acccgcacgc agaattggca caacggtaat tttcttacct ttgatctggt
   134641 cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg aggtcggcgg
   134701 tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc
   134761 tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt ttgacttcca
   134821 cgatcttcat actctttctc ctttgagggg cagccacaaa aaaaatcgaa ggattatacc
   134881 tcctttcttc aaggcggcaa tattattttc gttgacttta gtcaaaatga taacagtttg
   134941 agataaagtt attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa
   135001 cctttgccct ggcacaagtc ttctttcgcc gcgcgcccgg ggaaaagacg tgcaaaaagg
   135061 ttgtgtaaaa cagtctcgca aacgtttgct ttccctgtta gaattgcgcc gaattttatt
   135121 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
   135181 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
   135241 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
   135301 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
   135361 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
   135421 catgtgcgtt aatgacctgg tggtgcaagg tgcggaaccg ctgtttttcc tcgactatta
   135481 cgcaaccgga aaactggatg ttgataccgc ttcagcagtg atcagcggca tcgcggaagg
   135541 ttgtctgcaa tcgggctgtt ctctggtggg tggcgaaacg gcagaaatgc cggggatgta
   135601 tcacggtgaa gattacgatg tcgcgggttt ctgcgtgggc gtggtagaaa aatcagaaat
   135661 catcgacggc tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc
   135721 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
   135781 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
   135841 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
   135901 cggcggcggc ttctgggaaa acattccgcg cgtattgcca gataataccc aggcagtgat
   135961 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg caggtaacgt
   136021 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc
   136081 tgctccggaa gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa
   136141 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
   136201 gtggtgctta tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
   136261 aacaaaatta aaggcaccgt acgggcagtt ttcagcaata aggccgacgc gttcggcctt
   136321 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
   136381 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
   136441 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
   136501 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
   136561 gcgctggaaa atggcgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
   136621 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
   136681 gacatcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
   136741 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
   136801 ccgccgcaga gctacgctgc cgacgagtaa tgcccccgta gttaaagcgc cagctctgcc
   136861 gctggcgttt ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc
   136921 tggcatagtt ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa
   136981 taaaacggag taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt
   137041 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
   137101 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
   137161 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa cgctcattcc
   137221 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc
   137281 ctctacaacg agctattgct ggagatggcg cgcaaccaga tcttcctgat taatgaacgc
   137341 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
   137401 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
   137461 tacacctatc tggcggtgga aattatccgt agcgatagca tccgttacgc gctgctggag
   137521 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcgcc gcgtcgacgc
   137581 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
   137641 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
   137701 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
   137761 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
   137821 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
   137881 tatcataatt ttaaagactt tattaatttt cccaatgtcg gcaaagccaa tctggtgaac
   137941 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
   138001 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgggcatgtg
   138061 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
   138121 taccgcgtgg cgaaagattc acgcatcatc gactcgatga tccacgccgc acataacggt
   138181 aagaaagtca ctgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
   138241 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
   138301 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca
   138361 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
   138421 ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac
   138481 ccataccgcc cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg
   138541 ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc
   138601 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc
   138661 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
   138721 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
   138781 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
   138841 atgacgcgca atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg
   138901 aagcagcggg tgctggacat catcgacata ttgttcagcg atacggtcaa agcacgttat
   138961 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
   139021 cagttggcga tttacgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat
   139081 acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt
   139141 tcacatggtc atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca
   139201 gcgggtgcat ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac
   139261 gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag
   139321 cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa
   139381 acgcgcggaa aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg
   139441 tctgattttt atgggcgtgg aacataccca accggaaaaa ggtcgcaaac tggttattga
   139501 tattggcggc ggatctacgg aactggtgat tggtgaaaat ttcgaaccta ttctcgttga
   139561 aagccgccgg atgggttgtg tcagctttgc ccagctttat tttcctggcg gggtcatcaa
   139621 taaagagaat tttcagcgcg ctcgcatggc ggcagcacaa aaactggaaa ctttaacctg
   139681 gcaattccgt attcagggct ggaacgttgc aatgggcgct tccggtacca taaaagccgc
   139741 ccatgaagtg ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga
   139801 aaaactggta aaagaaattt tacgtcaccg taatttcgca tcgctgagtt taccgggtct
   139861 ttccgaagag cggaaaacag tcttcgttcc tggactggcg attttatgtg gtgtgtttga
   139921 tgctttagcc atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta
   139981 tgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc
   140041 caaccagtat cacatagaca gcgaacaggc ccgacgagtg ctggatacca ctatgcaaat
   140101 gtacgaacag tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact
   140161 gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg
   140221 ccactccgct tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct
   140281 gatgatggcg acactgctgc gctatcaccg taaagcgatt aagctcgacg atctgccgcg
   140341 ctttaccttg tttaagaaga aacagttcct gccactgata cagctattgc gccttggcgt
   140401 attactaaac aatcaacgtc aggcaaccac cacaccgtca acattgacac tgattactga
   140461 tgacagtcac tggacactgc gtttcccgca tgactggttt agtcagaatg cgctggtact
   140521 gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat
   140581 tgaagaagaa agtaccccag aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc
   140641 ggcccgcatt attcaggcac tttcgcgaat gggttcgatt tcattcagcg tatcaattaa
   140701 cggttgcggc ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc
   140761 ctcgcggatc tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc
   140821 cagatggcaa atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat
   140881 aaagctgccg tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc
   140941 gtagccggtg ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa
   141001 ggtgatttgc gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag
   141061 ttgccacgct tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc
   141121 ctgacatacc gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct
   141181 gttttcagcc ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa
   141241 accaaattcg tgcgcgaccg gcaagaagct atcggggccg atcagttcat cattctcacc
   141301 tttcatgcgc agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc
   141361 catcaggaaa aaatggttgt gctccagcgc ctgctgtagt cgattcatca tcgcgacttt
   141421 atctttcaat tcgcgttgca aatacattgc cccgcaacgc tgcatatttt ccggggcgtt
   141481 ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt
   141541 cactggcgag cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc
   141601 ccaaaagaaa cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga
   141661 ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg
   141721 ttccagcaag ggtgacagcc agtgagaaag tttttgcttg
//
LOCUS       DAJTNM010000002       111704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTNM010000002 DAJTNM010000000
VERSION     DAJTNM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 111704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 111704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.002"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            606..2891
                     /gene="nrdA"
                     /locus_tag="OO172_000134"
     CDS             606..2891
                     /gene="nrdA"
                     /locus_tag="OO172_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5510452.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3125..4255
                     /gene="nrdB"
                     /locus_tag="OO172_000135"
     CDS             3125..4255
                     /gene="nrdB"
                     /locus_tag="OO172_000135"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5510453.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4255..4509
                     /gene="yfaE"
                     /locus_tag="OO172_000136"
     CDS             4255..4509
                     /gene="yfaE"
                     /locus_tag="OO172_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5510454.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="OO172_000137"
     CDS             complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="OO172_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5510455.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5377..5619
                     /locus_tag="OO172_000138"
                     /pseudo
     CDS             5377..5619
                     /locus_tag="OO172_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="OO172_000139"
     CDS             complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="OO172_000139"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5510456.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="OO172_000140"
     CDS             complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="OO172_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5510457.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8373..10001
                     /gene="glpA"
                     /locus_tag="OO172_000141"
     CDS             8373..10001
                     /gene="glpA"
                     /locus_tag="OO172_000141"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5510458.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9991..11250
                     /gene="glpB"
                     /locus_tag="OO172_000142"
     CDS             9991..11250
                     /gene="glpB"
                     /locus_tag="OO172_000142"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5510459.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11247..12437
                     /gene="glpC"
                     /locus_tag="OO172_000143"
     CDS             11247..12437
                     /gene="glpC"
                     /locus_tag="OO172_000143"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5510460.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12630..13529
                     /locus_tag="OO172_000144"
     CDS             12630..13529
                     /locus_tag="OO172_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5510461.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13533..13769
                     /locus_tag="OO172_000145"
                     /pseudo
     CDS             13533..13769
                     /locus_tag="OO172_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13810..14280)
                     /locus_tag="OO172_000146"
     CDS             complement(13810..14280)
                     /locus_tag="OO172_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5510462.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14335..15537)
                     /locus_tag="OO172_000147"
     CDS             complement(14335..15537)
                     /locus_tag="OO172_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5510463.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSSRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15624..16166)
                     /locus_tag="OO172_000148"
     CDS             complement(15624..16166)
                     /locus_tag="OO172_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510464.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16445..16870
                     /gene="nudI"
                     /locus_tag="OO172_000149"
     CDS             16445..16870
                     /gene="nudI"
                     /locus_tag="OO172_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5510465.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16909..17511)
                     /locus_tag="OO172_000150"
     CDS             complement(16909..17511)
                     /locus_tag="OO172_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5510466.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNMVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17819..18958
                     /gene="arnB"
                     /locus_tag="OO172_000151"
     CDS             17819..18958
                     /gene="arnB"
                     /locus_tag="OO172_000151"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5510467.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18962..19930
                     /gene="arnC"
                     /locus_tag="OO172_000152"
     CDS             18962..19930
                     /gene="arnC"
                     /locus_tag="OO172_000152"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5510468.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19930..21912
                     /gene="arnA"
                     /locus_tag="OO172_000153"
     CDS             19930..21912
                     /gene="arnA"
                     /locus_tag="OO172_000153"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR5510469.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDFVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21909..22799
                     /locus_tag="OO172_000154"
     CDS             21909..22799
                     /locus_tag="OO172_000154"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5510470.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22799..24451
                     /gene="arnT"
                     /locus_tag="OO172_000155"
     CDS             22799..24451
                     /gene="arnT"
                     /locus_tag="OO172_000155"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5510471.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDLWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24448..24783
                     /gene="arnE"
                     /locus_tag="OO172_000156"
     CDS             24448..24783
                     /gene="arnE"
                     /locus_tag="OO172_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5510472.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24783..25169
                     /gene="arnF"
                     /locus_tag="OO172_000157"
     CDS             24783..25169
                     /gene="arnF"
                     /locus_tag="OO172_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5510473.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25163..25435)
                     /gene="pmrD"
                     /locus_tag="OO172_000158"
     CDS             complement(25163..25435)
                     /gene="pmrD"
                     /locus_tag="OO172_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5510474.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25540..26895)
                     /gene="menE"
                     /locus_tag="OO172_000159"
     CDS             complement(25540..26895)
                     /gene="menE"
                     /locus_tag="OO172_000159"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5510475.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADVLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26892..27854)
                     /gene="menC"
                     /locus_tag="OO172_000160"
     CDS             complement(26892..27854)
                     /gene="menC"
                     /locus_tag="OO172_000160"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5510476.1"
                     /translation="MRSAQVYRWQIPMDAGVLLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27854..28711)
                     /gene="menB"
                     /locus_tag="OO172_000161"
     CDS             complement(27854..28711)
                     /gene="menB"
                     /locus_tag="OO172_000161"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5510477.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28726..29484)
                     /gene="menH"
                     /locus_tag="OO172_000162"
     CDS             complement(28726..29484)
                     /gene="menH"
                     /locus_tag="OO172_000162"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5510478.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29481..31151)
                     /gene="menD"
                     /locus_tag="OO172_000163"
     CDS             complement(29481..31151)
                     /gene="menD"
                     /locus_tag="OO172_000163"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5510479.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31240..32535)
                     /gene="menF"
                     /locus_tag="OO172_000164"
     CDS             complement(31240..32535)
                     /gene="menF"
                     /locus_tag="OO172_000164"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5510480.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32614..32919)
                     /gene="elaB"
                     /locus_tag="OO172_000165"
     CDS             complement(32614..32919)
                     /gene="elaB"
                     /locus_tag="OO172_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5510481.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32974..33435)
                     /locus_tag="OO172_000166"
     CDS             complement(32974..33435)
                     /locus_tag="OO172_000166"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5510482.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33500..34417
                     /gene="rbn"
                     /locus_tag="OO172_000167"
     CDS             33500..34417
                     /gene="rbn"
                     /locus_tag="OO172_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5510483.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TAQRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34605..35825
                     /gene="elaD"
                     /locus_tag="OO172_000168"
     CDS             34605..35825
                     /gene="elaD"
                     /locus_tag="OO172_000168"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5510484.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGNEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSELMGKMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
     gene            36245..36860
                     /locus_tag="OO172_000169"
                     /pseudo
     CDS             36245..36860
                     /locus_tag="OO172_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            36963..37466
                     /locus_tag="OO172_000170"
     CDS             36963..37466
                     /locus_tag="OO172_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCR5510485.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(37533..38990)
                     /gene="nuoN"
                     /locus_tag="OO172_000171"
     CDS             complement(37533..38990)
                     /gene="nuoN"
                     /locus_tag="OO172_000171"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5510486.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFDYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(38997..40526)
                     /gene="nuoM"
                     /locus_tag="OO172_000172"
     CDS             complement(38997..40526)
                     /gene="nuoM"
                     /locus_tag="OO172_000172"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5510487.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(40690..42531)
                     /gene="nuoL"
                     /locus_tag="OO172_000173"
     CDS             complement(40690..42531)
                     /gene="nuoL"
                     /locus_tag="OO172_000173"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5510488.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAAQV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(42528..42830)
                     /gene="nuoK"
                     /locus_tag="OO172_000174"
     CDS             complement(42528..42830)
                     /gene="nuoK"
                     /locus_tag="OO172_000174"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5510489.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(42827..43381)
                     /gene="nuoJ"
                     /locus_tag="OO172_000175"
     CDS             complement(42827..43381)
                     /gene="nuoJ"
                     /locus_tag="OO172_000175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5510490.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(43393..43935)
                     /gene="nuoI"
                     /locus_tag="OO172_000176"
     CDS             complement(43393..43935)
                     /gene="nuoI"
                     /locus_tag="OO172_000176"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5510491.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(43950..44927)
                     /gene="nuoH"
                     /locus_tag="OO172_000177"
     CDS             complement(43950..44927)
                     /gene="nuoH"
                     /locus_tag="OO172_000177"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5510492.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(44924..47650)
                     /gene="nuoG"
                     /locus_tag="OO172_000178"
     CDS             complement(44924..47650)
                     /gene="nuoG"
                     /locus_tag="OO172_000178"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5510493.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(47703..49040)
                     /gene="nuoF"
                     /locus_tag="OO172_000179"
     CDS             complement(47703..49040)
                     /gene="nuoF"
                     /locus_tag="OO172_000179"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5510494.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(49037..49537)
                     /gene="nuoE"
                     /locus_tag="OO172_000180"
     CDS             complement(49037..49537)
                     /gene="nuoE"
                     /locus_tag="OO172_000180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5510495.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(49540..51342)
                     /gene="nuoC"
                     /locus_tag="OO172_000181"
     CDS             complement(49540..51342)
                     /gene="nuoC"
                     /locus_tag="OO172_000181"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5510496.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(51436..52098)
                     /gene="nuoB"
                     /locus_tag="OO172_000182"
     CDS             complement(51436..52098)
                     /gene="nuoB"
                     /locus_tag="OO172_000182"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5510497.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(52114..52557)
                     /gene="nuoA"
                     /locus_tag="OO172_000183"
     CDS             complement(52114..52557)
                     /gene="nuoA"
                     /locus_tag="OO172_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5510498.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(53188..54126)
                     /gene="lrhA"
                     /locus_tag="OO172_000184"
     CDS             complement(53188..54126)
                     /gene="lrhA"
                     /locus_tag="OO172_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5510499.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            55080..56297
                     /gene="alaA"
                     /locus_tag="OO172_000185"
     CDS             55080..56297
                     /gene="alaA"
                     /locus_tag="OO172_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5510500.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            56381..56980
                     /gene="yfbR"
                     /locus_tag="OO172_000186"
     CDS             56381..56980
                     /gene="yfbR"
                     /locus_tag="OO172_000186"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5510501.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(57039..58871)
                     /locus_tag="OO172_000187"
     CDS             complement(57039..58871)
                     /locus_tag="OO172_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5510502.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(58958..59608)
                     /gene="hxpA"
                     /locus_tag="OO172_000188"
     CDS             complement(58958..59608)
                     /gene="hxpA"
                     /locus_tag="OO172_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5510503.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALSGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(59619..60113)
                     /locus_tag="OO172_000189"
     CDS             complement(59619..60113)
                     /locus_tag="OO172_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5510504.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(60196..60651)
                     /locus_tag="OO172_000190"
     CDS             complement(60196..60651)
                     /locus_tag="OO172_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5510505.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            60989..62191
                     /gene="ackA"
                     /locus_tag="OO172_000191"
     CDS             60989..62191
                     /gene="ackA"
                     /locus_tag="OO172_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5510506.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            62266..64410
                     /gene="pta"
                     /locus_tag="OO172_000192"
     CDS             62266..64410
                     /gene="pta"
                     /locus_tag="OO172_000192"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5510507.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            64600..66120
                     /gene="yfcC"
                     /locus_tag="OO172_000193"
     CDS             64600..66120
                     /gene="yfcC"
                     /locus_tag="OO172_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5510508.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(66153..66695)
                     /gene="yfcD"
                     /locus_tag="OO172_000194"
     CDS             complement(66153..66695)
                     /gene="yfcD"
                     /locus_tag="OO172_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5510509.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(66753..67304)
                     /gene="yfcE"
                     /locus_tag="OO172_000195"
     CDS             complement(66753..67304)
                     /gene="yfcE"
                     /locus_tag="OO172_000195"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5510510.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(67360..68004)
                     /gene="yfcF"
                     /locus_tag="OO172_000196"
     CDS             complement(67360..68004)
                     /gene="yfcF"
                     /locus_tag="OO172_000196"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5510511.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            68140..68787
                     /gene="yfcG"
                     /locus_tag="OO172_000197"
     CDS             68140..68787
                     /gene="yfcG"
                     /locus_tag="OO172_000197"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5510512.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            68844..69206
                     /gene="folX"
                     /locus_tag="OO172_000198"
     CDS             68844..69206
                     /gene="folX"
                     /locus_tag="OO172_000198"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5510513.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            69227..70120
                     /locus_tag="OO172_000199"
     CDS             69227..70120
                     /locus_tag="OO172_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5510514.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(70168..71058)
                     /gene="rpnB"
                     /locus_tag="OO172_000200"
     CDS             complement(70168..71058)
                     /gene="rpnB"
                     /locus_tag="OO172_000200"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5510515.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIA
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(71255..72028)
                     /gene="hisP"
                     /locus_tag="OO172_000201"
     CDS             complement(71255..72028)
                     /gene="hisP"
                     /locus_tag="OO172_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5510516.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEACERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(72036..72752)
                     /locus_tag="OO172_000202"
     CDS             complement(72036..72752)
                     /locus_tag="OO172_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5510517.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(72749..73435)
                     /gene="hisQ"
                     /locus_tag="OO172_000203"
     CDS             complement(72749..73435)
                     /gene="hisQ"
                     /locus_tag="OO172_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5510518.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(73525..74307)
                     /gene="hisJ"
                     /locus_tag="OO172_000204"
     CDS             complement(73525..74307)
                     /gene="hisJ"
                     /locus_tag="OO172_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5510519.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(74528..75310)
                     /gene="argT"
                     /locus_tag="OO172_000205"
     CDS             complement(74528..75310)
                     /gene="argT"
                     /locus_tag="OO172_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5510520.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(75576..76145)
                     /gene="ubiX"
                     /locus_tag="OO172_000206"
     CDS             complement(75576..76145)
                     /gene="ubiX"
                     /locus_tag="OO172_000206"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5510521.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(76240..77757)
                     /gene="purF"
                     /locus_tag="OO172_000207"
     CDS             complement(76240..77757)
                     /gene="purF"
                     /locus_tag="OO172_000207"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5510522.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(77794..78282)
                     /gene="cvpA"
                     /locus_tag="OO172_000208"
     CDS             complement(77794..78282)
                     /gene="cvpA"
                     /locus_tag="OO172_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5510523.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(78541..79203)
                     /gene="dedD"
                     /locus_tag="OO172_000209"
     CDS             complement(78541..79203)
                     /gene="dedD"
                     /locus_tag="OO172_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5510524.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(79193..80461)
                     /gene="folC"
                     /locus_tag="OO172_000210"
     CDS             complement(79193..80461)
                     /gene="folC"
                     /locus_tag="OO172_000210"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5510525.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(80531..81445)
                     /gene="accD"
                     /locus_tag="OO172_000211"
     CDS             complement(80531..81445)
                     /gene="accD"
                     /locus_tag="OO172_000211"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5510526.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(81601..82260)
                     /locus_tag="OO172_000212"
     CDS             complement(81601..82260)
                     /locus_tag="OO172_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5510527.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(82343..83155)
                     /gene="truA"
                     /locus_tag="OO172_000213"
     CDS             complement(82343..83155)
                     /gene="truA"
                     /locus_tag="OO172_000213"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5510528.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(83155..84168)
                     /locus_tag="OO172_000214"
     CDS             complement(83155..84168)
                     /locus_tag="OO172_000214"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5510529.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(84234..85370)
                     /gene="pdxB"
                     /locus_tag="OO172_000215"
     CDS             complement(84234..85370)
                     /gene="pdxB"
                     /locus_tag="OO172_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5510530.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADTDALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            85469..86464
                     /gene="flk"
                     /locus_tag="OO172_000216"
     CDS             85469..86464
                     /gene="flk"
                     /locus_tag="OO172_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5510531.1"
                     /translation="MIQPISGPPLGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(86461..87639)
                     /locus_tag="OO172_000217"
     CDS             complement(86461..87639)
                     /locus_tag="OO172_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5510532.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLCFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(87914..89134)
                     /gene="fabB"
                     /locus_tag="OO172_000218"
     CDS             complement(87914..89134)
                     /gene="fabB"
                     /locus_tag="OO172_000218"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5510533.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            89293..91299
                     /gene="mnmC"
                     /locus_tag="OO172_000219"
     CDS             89293..91299
                     /gene="mnmC"
                     /locus_tag="OO172_000219"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5510534.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(91420..91698)
                     /locus_tag="OO172_000220"
     CDS             complement(91420..91698)
                     /locus_tag="OO172_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5510535.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(91732..92280)
                     /gene="epmC"
                     /locus_tag="OO172_000221"
     CDS             complement(91732..92280)
                     /gene="epmC"
                     /locus_tag="OO172_000221"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5510536.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(92280..93089)
                     /locus_tag="OO172_000222"
     CDS             complement(92280..93089)
                     /locus_tag="OO172_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5510537.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(93089..93913)
                     /gene="mepA"
                     /locus_tag="OO172_000223"
     CDS             complement(93089..93913)
                     /gene="mepA"
                     /locus_tag="OO172_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5510538.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(93917..95002)
                     /gene="aroC"
                     /locus_tag="OO172_000224"
     CDS             complement(93917..95002)
                     /gene="aroC"
                     /locus_tag="OO172_000224"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5510539.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(95037..95969)
                     /gene="prmB"
                     /locus_tag="OO172_000225"
     CDS             complement(95037..95969)
                     /gene="prmB"
                     /locus_tag="OO172_000225"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5510540.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            96135..96686
                     /gene="smrB"
                     /locus_tag="OO172_000226"
     CDS             96135..96686
                     /gene="smrB"
                     /locus_tag="OO172_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5510541.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(96790..97680)
                     /locus_tag="OO172_000227"
     CDS             complement(96790..97680)
                     /locus_tag="OO172_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5510542.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(97745..98191)
                     /locus_tag="OO172_000228"
     CDS             complement(97745..98191)
                     /locus_tag="OO172_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5510543.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNT"
     gene            complement(98188..98658)
                     /locus_tag="OO172_000229"
     CDS             complement(98188..98658)
                     /locus_tag="OO172_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5510544.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(98655..99203)
                     /locus_tag="OO172_000230"
     CDS             complement(98655..99203)
                     /locus_tag="OO172_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5510545.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(99208..99930)
                     /locus_tag="OO172_000231"
     CDS             complement(99208..99930)
                     /locus_tag="OO172_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5510546.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPYQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSC"
     gene            complement(99950..100672)
                     /locus_tag="OO172_000232"
     CDS             complement(99950..100672)
                     /locus_tag="OO172_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5510547.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATFTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(100732..102591)
                     /locus_tag="OO172_000233"
     CDS             complement(100732..102591)
                     /locus_tag="OO172_000233"
                     /inference="COORDINATES: protein motif:HMM:NF011812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCR5510548.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
     gene            complement(102673..103236)
                     /locus_tag="OO172_000234"
     CDS             complement(102673..103236)
                     /locus_tag="OO172_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5510549.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(103920..104405)
                     /gene="sixA"
                     /locus_tag="OO172_000235"
     CDS             complement(103920..104405)
                     /gene="sixA"
                     /locus_tag="OO172_000235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5510550.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(104608..106752)
                     /gene="fadJ"
                     /locus_tag="OO172_000236"
     CDS             complement(104608..106752)
                     /gene="fadJ"
                     /locus_tag="OO172_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5510551.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(106752..108062)
                     /gene="fadI"
                     /locus_tag="OO172_000237"
     CDS             complement(106752..108062)
                     /gene="fadI"
                     /locus_tag="OO172_000237"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5510552.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(108242..108526)
                     /locus_tag="OO172_000238"
     CDS             complement(108242..108526)
                     /locus_tag="OO172_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5510553.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            108898..110238
                     /gene="fadL"
                     /locus_tag="OO172_000239"
     CDS             108898..110238
                     /gene="fadL"
                     /locus_tag="OO172_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5510554.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            110604..111662
                     /locus_tag="OO172_000240"
     CDS             110604..111662
                     /locus_tag="OO172_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510555.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNCQAK
                     YQNEINQLSALGVNFDDI"
BASE COUNT        27990 a        29871 c        27461 g        26382 t
ORIGIN      
        1 tgcaatggct attttgttat tatttgatga ataatatcag tgcgtcataa ttcaagttaa
       61 taaccttaag ggataccagt tatatttaaa ctaaattaaa gtaatgaata attttcttat
      121 aatataaggt aaattaacaa aatggcttcg catttaacaa taaccgaata gaaaacaacc
      181 atttcgacat caacaatcgc ttacattcgc ttatatattg accacaactg atacatcaga
      241 ttatgtgatg actcgtgctt agatcaattt ttgcaatcat tagcaaaaag attaataaac
      301 catctatatc aatttatcca acctattctg ccgttcaaga aatcgccgaa cagttatttt
      361 taacaaattt ttctcttccc attgactttc ccggacacct tgtctgacct aaggtgcgcg
      421 aaagccactt tttccttcct gagttatcca caaagttatg cacttgcaag agggtcattt
      481 tcacactatc ttgcagtgaa tcccaaacat accccctata tatagtgttc taagcagctt
      541 cccttactac aggtagtctg catgaaacta ttgcggaaag aattccaaaa acaggtacga
      601 catacatgaa tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc
      661 tcgacaaaat ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt
      721 cccaggtcga gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc
      781 acgaaactat tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt
      841 atctcgccgc gcgcctggcg atcttccacc tgcgtaaaaa agcctacggc cagtttgagc
      901 cgcctgcgct gtacgaccac gtggtgaaaa tggtcgagat gggcaaatac gataatcatc
      961 tgctggaaga ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc
     1021 gtgatatgac cttctcttat gctgccgtta agcagctgga aggcaaatat ctggtacaga
     1081 accgcgtgac cggcgaaatc tacgaaagcg cccagttcct ttatattctt gttgccgcgt
     1141 gcctgttctc gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttacgacg
     1201 cggtttccac atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga
     1261 ctcgtcagtt cagctcctgc gtactgatcg agtgcggtga cagcctggat tccatcaacg
     1321 ccacctccag cgcgattgtg aaatatgttt cccagcgtgc cgggatcggc atcaacgccg
     1381 gacgtattcg tgcgctgggc agcccgattc gcggtggcga agcgttccat accggctgta
     1441 tcccgttcta caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg
     1501 gcggtgcggc aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt
     1561 tgaaaaacaa ccgtggggtt gaaggcaacc gcgtgcgtca tatggactac ggggtacaaa
     1621 tcaacaaact gatgtatacc cgcctgctga aaggtgaaga tatcaccctg ttcagcccgt
     1681 ccgacgtacc ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgcctgt
     1741 ataccaaata tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt
     1801 tctcgctgat gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc
     1861 actgtaatac ccatagtccg tttgatccga ccatcgcgcc agttcgccag tctaacctgt
     1921 gcctggaaat tgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa
     1981 tcgcgctgtg tacgctgtct gctttcaacc tgggcgcaat taatagcctg gatgaactgg
     2041 aagagctggc gattctggcg gttcgtgcac ttgacgcgct actggattat caggattacc
     2101 cgatcccggc cgccaaacgt ggagcgatgg ggcgtcgtac gctgggtatt ggtgtgatta
     2161 acttcgctta ctatctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc
     2221 tgacgcataa aaccttcgaa gcgattcagt attacctgct gaaagcgtct aatgagctgg
     2281 ctaaagagca aggcgcgtgc ccgtggttta acgaaaccac ttacgcgaaa ggtatcctgc
     2341 cgatcgatac ctataagaaa gatctggatg ccatcgctaa tgagccgctg cattacgact
     2401 gggaagcatt gcgtgaatca atcaaaacgc acggcctgcg taactccacg ctttctgccc
     2461 tgatgccgtc cgagacctct tcgcagatct ctaacgccac taacggtatt gaaccgccgc
     2521 gcggttacgt cagcatcaaa gcgtcgaaag acggtatttt gcgccaggtg gtgccggact
     2581 acgagcacct gcacgacgcc tatgagctgc tgtgggaaat gccgggtaac gatggctacc
     2641 tgcaactggt aggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact
     2701 acgatccgtc acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc
     2761 tcaccgccta caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgctg
     2821 aagacgcgca ggacgatctg gtaccgtcaa tccaggacga tggctgcgaa agcggcgcat
     2881 gtaagatctg atattgagat gccggatgcg gcgtaaacgc cttatccggc ctacggctcg
     2941 gtttgtaggc ctgataagac gcgccagcgt cgcatcaggc tccgggtgcc ggatgcagcg
     3001 tgaacgcctt atccggccta cggctcggat ttgtaggcct gataagacgc gccagcgtcg
     3061 catcaggcac aggatgcggc gtaaaatgcc ttatccggca ttaaactccc aacaggacac
     3121 actcatggca tataccacct tttcacagac gaaaaatgat cagctcaaag aaccgatgtt
     3181 ctttggtcag ccggtcaacg tggctcgcta cgatcagcaa aaatatgaca tcttcgaaaa
     3241 gctgatcgaa aagcagctct ctttcttctg gcgtccggaa gaagttgacg tctcccgcga
     3301 ccgtatagat taccaggcgc tgccggagca cgaaaaacac atctttatca gcaacctgaa
     3361 atatcagacg ctgctggatt ccattcaggg tcgtagcccg aacgtggcgc tattgccgct
     3421 tatttctatt ccggaactgg aaacctgggt cgaaacctgg gcgttctcag aaacgattca
     3481 ttcccgttcc tatactcata tcattcgtaa tatcgttaac gatccgtctg ttgtgtttga
     3541 cgatatcgtc accaacgagc agatccagaa acgtgcggaa gggatctcca gctattacga
     3601 tgaactgatc gaaatgacca gctactggca ccttctgggc gaaggcaccc acaccgttaa
     3661 cggtaaaacc gtaaccgtta gcctgcgcga gctgaagaaa aaactgtatc tctgcctgat
     3721 gagcgttaac gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt cttttgcctt
     3781 tgcagaacgc gaactgatgg aaggtaacgc caagattatt cgcctgattg cccgcgatga
     3841 agccctgcac ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg gcgcggatga
     3901 tccggagatg gcggaaattg ccgaagaatg taagcaggag tgctatgacc tgtttgtcca
     3961 ggcggcacag caagagaaag actgggcgga ttatctgttc cgtgacggtt cgatgattgg
     4021 tctgaataaa gatattctct gccagtacgt tgaatacatc accaatatcc gcatgcaggc
     4081 agtcggtttg gatctgccgt tccagacgcg ctccaacccg atcccgtgga tcaacacctg
     4141 gctggtgtct gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt cttatctggt
     4201 cgggcagatt gacgcggaag tggacaccga cgatttgagt aacttccagc tctgatggcc
     4261 cgcgttaccc tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca cccttccctt
     4321 ctggcggcgc tggaatccca caatgtggcg gttgagtacc agtgccgcga aggttactgc
     4381 ggctcctgtc gcacccgact ggtcgcaggt caggtggact ggattgccga accgttagcc
     4441 tttattcagc cgggggaaat tttgccctgt tgttgccggg caaaaggcga tattgaaatc
     4501 gagttgtgat cgggtactag cgccagatac gacgcttacg cgtattatct ggcctacaac
     4561 gattacatgg cgtagtaata cgctttcacc tgctcccagt ccgctttagg gatcggctcc
     4621 agatattttt ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg caagcggcga
     4681 cgacttttct ccagatcaag aaaaccagct tctgccttac cttctgtttt cacatagata
     4741 tggcgaacat agcaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagt
     4801 gccactgctt tcaacatggc ttgccgtact tcgtcagaat aaggcgatac tgcatgccgg
     4861 gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc
     4921 cattcaccct caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc
     4981 cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggcc gaacggataa
     5041 cgtactgaat gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc
     5101 tcgacgcatt gcaccctgct cataccatta cggcgatagt taggctcttc aacccagtcg
     5161 ccctctgttg cccaccagtg attaaattcg tcgtactttg ctgaaactgc cataccctat
     5221 cgcctgtcat ttttattaac gacaatgact ataggtggtt acctgagcaa aatcttaatg
     5281 aaacgtgtcg tattccgcta caggtgtgac accgcttttg cccttcattt gcagcgcata
     5341 gattccacca gccactacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag
     5401 tcggatacag cgatgaattt tattcgacag ggattaggca tcgccttaca accagagtta
     5461 acgctgaaaa gcattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
     5521 cagatttcgt tgctgactaa agaaaagccg gtagaaggca gtccactgtt tttactacaa
     5581 atgtgcatgg aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg
     5641 aacgccatcc tgcagtaaca ttactcttta ttaagaaatt ttactgcctt atcagggaaa
     5701 tcagtaaaca gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca
     5761 tcaggagtgt attcaggcag tttatctgac cgcacggtat aaggatgcac taccagttta
     5821 ttctgctgag catcttgcac catgccagtg agtttgatat tacccggctg cgatgtctcc
     5881 tcaatcaaca tatggtaatc cggaccaata ccatctgcat attccgccac ctgtttcatg
     5941 gcacccggct taaacatcca gtcgtagttg taattaaccc agcttccatc cggctgtttc
     6001 tgctgcgttt cattccagtc ggtataggca atcagctgta ccagattgag ctccatgccc
     6061 attttgggtt ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa
     6121 taaactttat cgtctttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc
     6181 gcaatatcct tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg
     6241 atatttttcc cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg
     6301 tgcacccgga agtcggactt acccattggg aaacgccccg gataagtctg tactttttta
     6361 ccgttttcaa tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa
     6421 tctatcgcgt agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca
     6481 gtaacacgat cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc
     6541 aaatcctgct ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta
     6601 tgctccggca aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc
     6661 gccgccattg cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt
     6721 ttcagcttca atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg
     6781 acgtttttcg ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat
     6841 cattaccata aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct
     6901 cgccgccacc gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc
     6961 cgcttttttc ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat
     7021 caggaagccg ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat
     7081 ccagtaaacg atagtcgcga tggtcaccag tgtcataaag aagacgccgg ttgccccacg
     7141 gttaccacgg aagactttgt ccgacatcca gccgcacagc agggtgcccg gaatacccgc
     7201 atactcatag aagaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt
     7261 cagataagtc ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc
     7321 gatggcgatg taccacagca gtttgttcgg cagcacgtac tgcatgaaga tttgcttcgc
     7381 ggtcagctcc tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc
     7441 gatcggcggc aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc
     7501 caccagaatg gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc
     7561 catccccagc aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca
     7621 cactgacaca atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca
     7681 cggcggccac cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc
     7741 aatgctcgac gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat
     7801 caaacctgcg ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa
     7861 tttcgaaaat ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga
     7921 gaatccctgc tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta
     7981 agccgcatag ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg
     8041 atcgatctcc gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat
     8101 tgatagcctc cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac
     8161 ctgccgtttc ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc
     8221 cgcacacatt attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc
     8281 actttcgaat tatgatcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta
     8341 aatggtaaaa aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg
     8401 acgtgattat cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc
     8461 gcgggctgcg cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta
     8521 accacggcct gctgcacagc ggtgcgcgct atgcagtaac cgatgcggaa tcggcccgcg
     8581 aatgcattag tgaaaaccag atcctgaaac gcattgcaca tcactgcgtt gaaccaacca
     8641 acggcctgtt tatcaccctg ccggaagatg acctctcatt ccaggccact tttattcgcg
     8701 cctgcgaaga agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg
     8761 aacctgccgt taacccggca ctgattggcg cggtgaaagt tccggatggc accgttgatc
     8821 catttcgtct gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta
     8881 ccgctcatga agtcacgggg ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac
     8941 gtaaccatct caccggcgaa actcaggccc ttcatgcacc tgtcgtggtt aatgccgctg
     9001 ggatctgggg gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga
     9061 aaggatcgct gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta
     9121 aaccttccga cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggtaccacct
     9181 ctttacgtat tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata
     9241 ttctgctgcg tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg
     9301 cctattctgg cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca
     9361 gccgtggcat cgtgttgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca
     9421 tcaccggtgg caaactgatg acctatcggc tgatggctga atgggctacc gacgcggtat
     9481 gccgcaaact gggcaacacg cgcccctgta cgactgccga tctggcactg cctggttcac
     9541 aagaacccgc tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt
     9601 ctgcggttta tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggc cgtctacacc
     9661 gtagcctggt atgtgagtgc gaagcggtaa ctgcgggtga agtgcagtac gcggtagaaa
     9721 atttaaacgt taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggcacct
     9781 gccagggcga actctgcgcc tgccgcgctg ccggactgct gcaacggttt aacgtcacga
     9841 cgtccgcgca atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc
     9901 aacccatcgc ctggggagac gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg
     9961 gactgtgtgg tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcggc
    10021 ggcctcgccg gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt
    10081 gtcacgcgtg gtcaaagcgc gttgcatttc tcatccggtt cgctggattt gctgagccat
    10141 ctgccagatg gtcaacccgt ggcagacatt cacagtggac tggaatcttt gcgtcagcag
    10201 gcacctgcac atccttactc tcttctcggg ccacaacgcg tgctcgatct cgcttgccag
    10261 gcgcaggcat taatcgctga aagcggtgcg caattgcagg gcagcgtaga actggctcac
    10321 cagcggatta cgccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc
    10381 gtctggccgc tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc
    10441 caggcgcatc ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aaccgcagaa
    10501 atagagctgc cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat
    10561 atcgcccgtt tccttgataa tgaagaaaac tggccgctgt tacttgatgc gcttattcct
    10621 gtcgccaata cctgcgaaat gatcctgatg cccgcctgct tcggcctagc cgatgacaaa
    10681 ctgtggcgtt ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg
    10741 ccttccgtgc tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt
    10801 ggagtgtgga tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat
    10861 gaaatctgga cccgcaatca cgccgatatt ccgctacgtc cacgtttcgc ggttctcgcc
    10921 agcggcagtt tctttagtgg cggactggta gcggaacgta acggcattcg cgagccgatt
    10981 ctcggccttg atgtgctaca aaccgccacg cggggtgaat ggtataaggg cgattttttt
    11041 gcgccgcaac cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcgcag
    11101 gcagggcaaa ccattgagaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc
    11161 atcgcccagg gatgcggcgg cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag
    11221 attgcccaac gcgcaggagg ccaacaatga acgacaccag cttcgaaaac tgcattaagt
    11281 gcaccgtctg caccaccgcc tgcccggtga gccgggtgaa tcccggttat ccagggccaa
    11341 aacaagccgg gccggatggc gagcgtctgc gtttgaaaga tggcgcactg tatgacgagg
    11401 cgctgaaata ttgcatcaac tgcaaacgtt gtgaagtcgc ctgcccgtcc gatgtgaaga
    11461 ttggcgatat tatccagcgc gcgcgggcga aatatgacac cacgcgcccg tcgctgcgta
    11521 attttgtgtt gagtcatacc gacctgatgg gtagcgtttc cacgccgttc gcaccaatcg
    11581 tcaacaccgc tacctcgctg aaaccggtgc ggcagctgct tgatgcggcg ttaaaaatcg
    11641 atcatcgtcg cacgctaccg aaatactcct tcggcacgtt ccgtcgctgg tatcgcagca
    11701 tagcggctca gcaagcacaa tataaagacc aggtcgcttt ctttcacggc tgcttcgtta
    11761 actacaacca tccgcagtta ggtaaagatt taattaaagt gctcaacgca atgggtaccg
    11821 gtgtacaact gctcagcaaa gaaaaatgct gcggcgtacc gctaatcgcc aacggcttta
    11881 ccgataaagc acgcaaacag gcaattacga atgtagagtc gatccgcgaa gctgtgggag
    11941 taaaaggcat tccggtgatt gccacctcct caacctgtac atttgccctg cgcgacgaat
    12001 acccggaagt gctgaatgtc gacaacaaag gcttgcgcga tcatatcgaa ctggcaaccc
    12061 gctggctgtg gcgcaagctg gacgaaggca aaacgttacc gctgaaaccg ctgccgctga
    12121 aagtggttta tcacactccg tgccatatgg aaaaaatggg ctggacgctc tacaccctgg
    12181 agctgttgcg taacatcccg gggcttgagt taacggtgct ggattcccag tgctgcggta
    12241 ttgcgggtac ttacggtttt aaaaaagaga actaccccac ctcacaagcc atcggcgcac
    12301 cactgttccg ccagatagaa gaaagcggcg cagatctggt ggtcaccgac tgcgaaacct
    12361 gtaaatggca gattgagatg tccacaagtc ttcgctgcga acatccgatt acgctactgg
    12421 cccaggcgct ggcttaaact cccttctgat gcccggtaag catgtggtta ccgggcattt
    12481 ttgcgtacac gattccgtgc ccaatgtatg cgttgcaacg cagtgaaaat tcctctgaaa
    12541 acgtctcgca aaggctgaaa ctggcagatg tcaaaggcct gggataaccg taatgtcgcg
    12601 tcatcataaa tatcaggtga cggacaacca tgaccgaatc aacaacctcc tccccgcatg
    12661 atgcggtatt taaaaccttt atattcacac ccgaaaccgc acgggatttt ctcgaaatac
    12721 atttaccaga accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta
    12781 gttttattga aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca
    12841 gcgacggtga cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata
    12901 tggcttttcg gccaatgcgc tatgccactg ccgccatgca gcgtcaccta gataaaggct
    12961 atgacagagt tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc
    13021 cgtactcact taactggctg gatgagtctg acgatccgca acttgcccgg cagttgtaca
    13081 ccgaagcttt tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc
    13141 ggcgtatagc tctgctggaa ctgattcaaa agcatattcg cgaccacgat ttaatcggca
    13201 tggtcgacag gatcaccacg cttttggtta gaggcttcac taatgacagc cagctacaaa
    13261 cactgtttaa ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattcagg
    13321 agattgccga acgttcacca ctacaaaagg agagattaat gactattgct gaacggctac
    13381 ggcaggaagg gcatcaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg
    13441 ccttgcgcat gctggagcag ggctttgaac gtgagattgt gctggcggca acccaactca
    13501 ctgatgctga tattccgaac tgttattaaa aggagaggga gagattgatg actattgctg
    13561 aacggctacg gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc
    13621 agcaaggtaa attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt
    13681 tgcgcatgct ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg
    13741 aagccgatct ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg
    13801 cctttgatat caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc
    13861 cagtcgttga tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt
    13921 cgctccccac gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc
    13981 tttacccgca gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg
    14041 cccggcgtta tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc
    14101 atcaatccct tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac
    14161 ctgcactaac agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca
    14221 gcgcgcagcc cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa
    14281 attttgccgg agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac
    14341 ggataaagtc atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc
    14401 gtttaaccaa cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc
    14461 caggctgtag cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc
    14521 tagcgcaaag ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa
    14581 atggttggcc cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga
    14641 aggaatcact tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa
    14701 accaccggtg aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact
    14761 gatctgcgcg ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt
    14821 aacgtccagc cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag
    14881 ttcgatgatc ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt
    14941 gtccaggctt tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac
    15001 cggcggctgt ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat
    15061 caccttaaat tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac
    15121 ggcaaaacca caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc
    15181 cgcttgttta cggctgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat
    15241 ctctttgagc caggcttcat gcagcacaag gccttcacct tttgctgtcg cagcggcgag
    15301 tgcgcttaaa tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg
    15361 ctgactacgt tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg
    15421 gcgagataat ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc
    15481 aacgatttgc ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt
    15541 tatctcctgc ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa
    15601 actgaatgcc gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca
    15661 gccacagcgt tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc
    15721 tcaatactga ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt
    15781 tgaataccgc tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg
    15841 ttgccaattt tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa
    15901 ttcgggttgg tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga
    15961 ttcaacccca gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg
    16021 cttaaagcca ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta
    16081 cccgcctggc cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc
    16141 agacctgcta gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc
    16201 ccaaagctgc gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct
    16261 agtcacttat tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact
    16321 ttaccgatac acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc
    16381 actttcacat atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac
    16441 ccctgtgcga caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct
    16501 gtgcaaaatg gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg
    16561 cgtggagtct ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg
    16621 agaacagctg cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa
    16681 gacatatgca gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt
    16741 ttctgccaac cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc
    16801 tgaagatctg gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg
    16861 tcttctttaa caatgatgac agccatcact ggctgtcagg ttgaaaattt agtggttaac
    16921 gaattcccca tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata
    16981 atcaggttta aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg
    17041 ggtgaaaata accatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata
    17101 aatctcatta ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa
    17161 ccaggtcgcc gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat
    17221 gtcagcgcta aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc
    17281 tgttttatct gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa
    17341 aacgacaacc gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa
    17401 accattgcta ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa
    17461 aatgatgaaa tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc
    17521 acctgtagac aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa
    17581 ttagcagttg gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc
    17641 caaatgcaaa gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag
    17701 tttcttaagg ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac
    17761 acaacactct ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat
    17821 gtcagaattt ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa
    17881 agaggttctc gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc
    17941 cttttgccag ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat
    18001 gcatatcacg ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct
    18061 gacctgggtt tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga
    18121 tgtcgaccgc gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg
    18181 cactaaagcc atcattccgg tgcattatgc cggtgcgcca gcagatattg acaccattcg
    18241 cgccattggc gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac
    18301 gtattacaaa gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat
    18361 taaaaatatt acctgtgctg aaggtggcct gattgtaact gataatgaaa accttgcccg
    18421 ccagctacgg atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg
    18481 gggccgtgca ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat
    18541 taacgccgcg attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg
    18601 cgaaattgcc cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct
    18661 tcccgcctgg ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg
    18721 ttgtggtatc agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg
    18781 gttacatttc cgcgccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc
    18841 gttaccgaat accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat
    18901 gactaccgcc gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc
    18961 catgtttgaa atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca
    19021 ggaaagctta ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga
    19081 gtatgagatc ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga
    19141 agcctcacaa gcggagaaca gccatattgt gtctattttg cttaaccgca attacgggca
    19201 acattcagcg attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga
    19261 tgccgatctc cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg
    19321 ttacgacgtg gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc
    19381 ttcgaagatg attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg
    19441 ttgtatgctg cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg
    19501 cagcaccttt atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt
    19561 acatcatgcc gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt
    19621 gatgtacgac ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg
    19681 cagcattatt gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac
    19741 cttcggacca caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt
    19801 tattggcgct cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac
    19861 cgatgtccgc gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga
    19921 aaatgaataa tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa
    19981 gctctgctgg ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt
    20041 gaaaaagcct tttatggttc ggtggctcat ctggcggcgg aaagagacat tccggtttat
    20101 gcgccggata acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag
    20161 gtgattttct ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc
    20221 gcaggtgcat ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg
    20281 aactgggtgc tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa
    20341 cgtgccgatg ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc
    20401 gctattacgc tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta
    20461 cccgccatta aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt
    20521 tttggtcgca gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg
    20581 cacaacatgg tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat
    20641 cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg
    20701 agcgtgattt ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc
    20761 accggacagg cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc
    20821 ctggtgcaag gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg
    20881 gtactcatcc tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc
    20941 gaagatcatt atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg
    21001 aatcatccac attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag
    21061 tatcacgtca aaaaatgtga tttcgtcttg ccgctggtgg cgatagccac gccgattgaa
    21121 tatacccgca acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc
    21181 cgctactgcg ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg
    21241 atgtgtagcg ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat
    21301 aaaccacgct ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat
    21361 ggcgaaaaag agggtttaca gttcaccctc ttccgcccgt ttaactggat gggaccacga
    21421 ctggataacc ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc
    21481 aatctggtag aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt
    21541 actgatatcc gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc
    21601 tgcgacggcg aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg
    21661 ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt
    21721 gcgggctttc gcgttgtcga aagtagcagc tactacggca aaggatatca ggacgtagag
    21781 catcgtaaac cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat
    21841 atgcaggaaa ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat
    21901 aaaccatcat gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg
    21961 aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt
    22021 tcagcgtcgg cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt
    22081 tgtggaagat gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag
    22141 gtacggcctg gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg
    22201 ctaaacatca cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg
    22261 gtaactggga tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag
    22321 agattatcgg tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg
    22381 tgatcgaagc aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatgc
    22441 cgttccgtcc actgctcgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac
    22501 ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac
    22561 tcaaccgcat cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag
    22621 gctgcgctta tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga
    22681 cattttgccc tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg
    22741 gaaatattgc cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat
    22801 gaaatcggta cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc
    22861 gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga
    22921 aatgctggca tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa
    22981 acccattgcc ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt
    23041 tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt
    23101 tactctgcgc ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc
    23161 attgtttatt gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg
    23221 gctggtggcg ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa
    23281 aagcgcagga tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt
    23341 tctcgccctt gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg
    23401 gaaagatctc tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc
    23461 ctggggactg gcgatagcgc agcgggagcc tgacttgtgg cactattttt tctgggttga
    23521 gcatattcaa cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt
    23581 gccggtcatc attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac
    23641 aggctggaaa aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc
    23701 gctgctgttt ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc
    23761 acctctggca atgctgttgg cgcattacgc tttgctggca gcaaaaaata atcctctggc
    23821 gctgcggatt aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt
    23881 tgtgatctcc ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa
    23941 agtcttttgt gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac
    24001 aaacgtcgaa aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt
    24061 aggattttca attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat
    24121 gacacaagaa tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc
    24181 aggtctggca tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt
    24241 gaaatacggc cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc
    24301 aaactggctt aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg
    24361 cgatgaagat atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg
    24421 tctggtgctg attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg
    24481 cttagcgttg ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa
    24541 cggcgcaaac atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg
    24601 gtgctctggc tgctggtctt gcagaacgta ccggtaggca ttgcctaccc gatgttaagt
    24661 ctgaattttg tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg
    24721 cgtcactggt gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg
    24781 taatgggcct gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc
    24841 tgggttttgc ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc
    24901 tgttagcttt tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt
    24961 ccgttttttg ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc
    25021 tcagtatgag ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga
    25081 ctttttcgct gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt
    25141 tcctgcccac gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc
    25201 actcatcggg gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat
    25261 taatacaata aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt
    25321 cactcttcac ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat
    25381 gtctgttatc ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc
    25441 tgtttgctaa gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc
    25501 aatggaatgg cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca
    25561 ctcttttagc gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt
    25621 cagccagcgc accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc
    25681 aacggtttgc tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc
    25741 aacggaaacg ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc
    25801 ttccggctga ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt
    25861 cagcttgcca ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac
    25921 cagtgaaacc agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca
    25981 cacttcatta ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag
    26041 gacgtcggct tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa
    26101 gcaacgaatc ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc
    26161 aagtaacacc gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt
    26221 tggcaccagt gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg
    26281 caccgtcatc cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg
    26341 aaacagcggt aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac
    26401 accttgcgca ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt
    26461 agaacccgag gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac
    26521 gccttcaacc agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg
    26581 caccagcgca aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag
    26641 ctgagggttc acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag
    26701 cgtttgcggc gtattccacg cccgcaacat cacgccgctg ccctcaacca caccctgcgc
    26761 cgcaaagccc gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc
    26821 gtcattaaga cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca
    26881 gtcagagaag atcataacaa ctgctccagt acatcagcgt ccactaacgg cagtgggctc
    26941 cccggccagc gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg
    27001 gtgtctggcg ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca
    27061 atggaagaac tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt
    27121 actttttcca gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca
    27181 gccacaaagg caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt
    27241 tcacgggcaa acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg
    27301 cggtggcgat aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag
    27361 gcgcgatttg cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc
    27421 accatgccgt cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc
    27481 ggcatatcgg caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca
    27541 cggtagttgg ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca
    27601 aaggccacgg aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag
    27661 gcaagcagca cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag
    27721 atctcccccc acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt
    27781 tttaaccgcc tgtcgcgcag aagcaccccc gcgtccatgg ggatctgcca gcggtatacc
    27841 tgcgcgctac gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt
    27901 tgaaagcgtt gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc
    27961 ccgccagctc ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc
    28021 agcgcagcgc catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt
    28081 ccagatccgc cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt
    28141 cgtactgacg gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca
    28201 tgtaggaagc gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga
    28261 tggcattatc tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga
    28321 tggagtagcc agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga
    28381 agtccagcac gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac
    28441 gcactttctg gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca
    28501 cgccgatatt gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca
    28561 gaggacggaa ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg
    28621 tggatttttc ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg
    28681 cgtaaggcat tgcttcatca ggataaatca tattgtgtcc tttagtcaaa aacgcaagat
    28741 ctgcgccaga cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg
    28801 aggaatgaca tggcagtcgg cagccagttc cgccgctagg gcgcggaatt tgctgtcacg
    28861 ttcaccacat aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg
    28921 ctggacggcg agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct
    28981 gcgcagcgcc accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg
    29041 ataccagtcg gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg
    29101 atcggaacgc tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc
    29161 gataataacc ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc
    29221 gccaagcgag taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg
    29281 cagtaagtcg gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc
    29341 tgggagatca acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc
    29401 gtggcaatcg ccggaaaaac cgtgcaaaaa caccagccag ggtaaacctg gttttccgtg
    29461 ttttgcctgc gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt
    29521 tgcgcaccat cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag
    29581 gcgtcggcaa atgccgtttc aagttcctgc cagttttgcg gacgatgata tttcagctcg
    29641 aacatcgcgg cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc
    29701 tcgctttgcg gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt
    29761 aataccagcg gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagtgcg
    29821 gagagatcgc ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg
    29881 gtcgaaagca gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc
    29941 ggaagttgcg aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc
    30001 ccctgttcag gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca
    30061 cggcgggcaa taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag
    30121 ggctggcgtt tttctgccgg atgcagctcc agccagtcgg caatattgcc aattaagcga
    30181 cgtccgcgat ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct
    30241 tctggttcac agcttgcctg ccattgcagg agccgtttcc ccgtcaggct gcttcccagt
    30301 tgcaccacaa tttgtgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga
    30361 tcggcacacg gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca
    30421 agagtttgcg cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc
    30481 accacgccgc gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga
    30541 ggcgcttcac gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag
    30601 ctaagcccgg tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg
    30661 actccccccg catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg
    30721 gggatatcct gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt
    30781 cccggctggc gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg
    30841 gttaagagaa tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt
    30901 gccaccgccg tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc
    30961 cccagcgcca gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa
    31021 ttctccgccg ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt
    31081 ctgacgccgt gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg
    31141 cttactgaca tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa
    31201 tagattattg atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt
    31261 acgcagccct gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg
    31321 gacaatgccc gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag
    31381 ggaaacgcag aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc
    31441 tcgggtgaac ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc
    31501 aactgctgcc gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga
    31561 agtccagata cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa
    31621 aacatccagc gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat
    31681 gttctcgcgc tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt
    31741 atcatcagga tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg
    31801 ccgccgccat aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat
    31861 gtaaaaatgg tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt
    31921 gaccggactt gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc
    31981 ttcggcgatg gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca
    32041 gtgttgttct cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt
    32101 ggcgataaat tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt
    32161 caaccgcagc gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt
    32221 gccctgcgac gggtcaaaag cattcagccc ccaaatgcgt aagtcggcgt gttccgggtg
    32281 ctggcgaaga aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac
    32341 ggcagcttct tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact
    32401 ggccagccag ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg
    32461 aatgccgggt gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt
    32521 agtaagtgat tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac
    32581 aaaaaaaatg cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag
    32641 taccagcccg acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc
    32701 atcagcacga taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac
    32761 ccgttttttc acatcatcca gcgctttttc tgcacgcgct ttcagctcaa catatttctg
    32821 atcggcgggc tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag
    32881 gtcgtcatcg atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa
    32941 aacctgtcac taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat
    33001 cccaatgtgt gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact
    33061 ctggtagaag ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg
    33121 atgcgtacat gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa
    33181 cgcttcactg acaatcaccc gacctataac gacaggctca agatcatcat cacttttcag
    33241 aatcctcgca tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc
    33301 cgtcaaatca tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc
    33361 gcaacgcagt tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc
    33421 ttgccattca atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt
    33481 tttgaaacgt gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac
    33541 ccgcacgcgc aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact
    33601 ttggttgttt gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc
    33661 tggaaaactg gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc
    33721 cggcttgctg tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc
    33781 gcaaggtatc cgtgaatttg tggaaaccgc gcagcggatt agcggctcat ggaccgatta
    33841 tccgctggaa attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt
    33901 aaccgcttat ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga
    33961 taaaccgggt gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact
    34021 gtttcaggaa ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg
    34081 cgcagattac ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg
    34141 cccctgcgat gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac
    34201 gctggatata accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc
    34261 tgcgacacta gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg
    34321 ctatgatgac aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac
    34381 tgaactggcg aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg
    34441 gtgaaacttg ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg
    34501 agcgattttt gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc
    34561 ttttcatctt gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg
    34621 tcagcaatta ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta
    34681 ctgtgaccga ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa
    34741 gtattgagtt actgcataat attgcgttag gttatgatga atttggaaaa aaagctgaag
    34801 atattcttta ccatattgtt agaaacccaa caaatgagac cctatcgatt atccgactta
    34861 ttaaaaatgc ctgtttaaaa ttatataatt tggcacatac cgcaaccaac tcccacctta
    34921 aaccaactgg tccggataat tcagatgttc tcctgttcaa aaagctattc tccccttcga
    34981 aattaatgac aattatcggt aatgaaattc ctcttatatc tgaaaaacag tcgctttcaa
    35041 aggtgctttt aaatgatgag aataatgaac tgagtgatgg tacaaacttc tgggataaaa
    35101 atcgtcaatt aaccacagat gaaatagctt gctatcttca gaagatcgcc gctaatgcaa
    35161 aaaatactca agtcaattat cctactggtc tctacgtccc ctactccacc agaactcacc
    35221 tggaagacgc tctcaatgaa aatattaaga gcgatccatc atggccgaat gaagtccagt
    35281 tattccccat aaatactggc ggacactgga tattagtttc gctacagaaa atagtaaata
    35341 aaaaaaataa taaactacaa ataaaatgcg tcatattcaa ctcattgcgt gcactaggcc
    35401 atgataaaga aaattcactt aagcgtgtca ttaatagttt taattctgaa ctcatgggaa
    35461 aaatgtcgaa taataatata aaagttcatt taaatgaacc agagataata tttttacatg
    35521 ccgatcttca gcaatactta agccaaagtt gcggtgcgtt tgtgtgcatg gcagcccagg
    35581 aagtgattga acaaagggaa agcaattctg acagcgcccc ctatacgtta ttaaaaaact
    35641 atgctgacag atttaaaaaa tattcagcag aagagcagta cgaaattgat tttcaacatc
    35701 gactggtaaa cagaaattgt tatttagata aatatggcga tgcacgaatc aatgcttctt
    35761 atacacaatt agaaataaaa cactcacaac ccaaaaatag agcatccggc aaaagagtga
    35821 gttaacatca ttgttaatca attcgaccag tcagtaaata tgctgactgg tcgaacataa
    35881 tttgctaaaa agtcatcact gactgatgtc agtcccacca tctgccagtt caatctacgg
    35941 gtatttccag cgtatcgcgg cacgcgattg ctggtctgtt gatgtagtgg attgtagttg
    36001 ctgacttttc ttaccctcag atttctgccc cacttgtcaa ttcaatctgc caataaataa
    36061 cataattata tttttattcc tcacagaaat tcctttttaa aaatagcgaa cccactgtaa
    36121 aaaacacttt ttgtatacct ctaccaagcg taataaaata aacgctcata attcattata
    36181 aataataatg aaataacgcc ttttattgat aatttacata tgaaaaaaat tttccttttg
    36241 ttatattatt tctgttttca ctccctctga ttatttttac caaccctggg tcactgcatt
    36301 atcaccaacg ccccgacacg taagtcctga gcaattagaa aaaacagttc gttatcttac
    36361 acaaactgtg catccacgta atgccggcaa tattgataat ctgaataggt ccgcagagta
    36421 tataaaagag ttctttgtca gtagcggtgc cagagttacc gcgcagggcg tccccgattg
    36481 gctattacga ctctgcgcct ggaagccaga attaccctta cccggcaatg tcctggcttt
    36541 atcccgatca gggagacttt attgctgtgg tcggcagaat gcaggatatc aacgccgttc
    36601 gtcaggtaaa agcggcattg ttgtcatccc gggatttatc tgtttattct atgaatgccc
    36661 cagggtttat tcccggtatt gatttttctg atcaccttaa ttattggcaa cacgatattc
    36721 ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg
    36781 ataccgcaga cagattgaat tatcagaaaa tggctcaggt ggtagatggt gttataacct
    36841 tgttatacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta
    36901 cattttattt tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat
    36961 aaatgggaat gattggctat tttgcagaaa ttgactcaga aaagattaac caactgcttg
    37021 agtctactga aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac
    37081 tggatattga taaacgctgg gattttttac actttgggct gacaggcacc tctgcttttg
    37141 atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttga tgaacacagc ctcgaagatg
    37201 gcatagatgg cttcctggga ttgacctgga atcaggaatt agccgcgaca atcgaccgac
    37261 tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa
    37321 tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc tttgcctcga
    37381 tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc
    37441 atgcgctgac ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat
    37501 gtcggcggct ttctgactta caaactaaca gtttacatca gcggcattgc taaccgcaca
    37561 atactaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agaaatcagc
    37621 accacgatac cgcccgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc
    37681 ggtgcgtgca ggtacaggct caccgccacg cgcaggtagt agtagaggcc gattgccgaa
    37741 ccgacaacca cggcacccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg
    37801 tagaacttac cgataaagcc cagcgtcatc gggataccgg ccagagacag catcatcacc
    37861 gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga gaacagggaa
    37921 tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa cgcgccgagg
    37981 ctgctgaaca gataaccggc caggtaaacc cctaccgctt ccatcgacat ctcgccggtt
    38041 tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga gtaaccgagc
    38101 agacgtttga tgttggtctg gctcaacgcc atcaggttac cgaagatgat ggaggcaaag
    38161 gcgataatcg ccagtactac gcgaatcgct tcgctgtcac ccaccggtgc gtagaggaac
    38221 agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc
    38281 ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt
    38341 ttgaagccga ggccaacaat catcatgccg aaacctgcca gcaacagcgg ctcgttgagc
    38401 ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac
    38461 accagcgcca taccgaacag caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg
    38521 atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc
    38581 aaagagatca gttcaatacc gaggaacaga gacgccagat ggtttgcatt cgccagcagg
    38641 atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag
    38701 ccttcaagcc acgggtagtc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg
    38761 gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc
    38821 tggccaacaa accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg
    38881 ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg
    38941 acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac
    39001 ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc
    39061 gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac
    39121 aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt
    39181 ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg aataaacaga tgcaaagacc
    39241 agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc gaacagaatc
    39301 ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac
    39361 agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg
    39421 cgggtatgga tacgttcata aagctgaccg cagagaataa acagacccgc cgccgacaag
    39481 ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagatt
    39541 gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg
    39601 gtctgagcga aggccatcca cgcaccgtag aagatgccga taacacccag ccacatagcg
    39661 attggcgcga actctgccga cgcgttcggg aacagcggca gggagaaacg caacaaaccg
    39721 taagcggcgg ttttcagcaa gatccccgcg aggtccacgg aaccggcggt cggagcctgg
    39781 gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg
    39841 atgaagaagc ccagcatcaa cagatattcc acaccactgg acattggcgt attcagcagc
    39901 tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg
    39961 atggcgatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt ggttgccacc
    40021 gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc
    40081 ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg
    40141 ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg
    40201 atctctttcc acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc
    40261 agcagcgaca gaccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc
    40321 atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc
    40381 tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag
    40441 cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga
    40501 attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg
    40561 accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg
    40621 atgcgccgcg aaggcagctt atccgaccta cgttcttata tgcccagggg caatcccaca
    40681 atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat
    40741 ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt tacctgcaaa
    40801 gcgggaaaga accgccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca
    40861 ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa agccccaggc
    40921 gttgtaccac caggtactca gcaggcggcc cggcgcactg ttggcgatgg aggtcaccag
    40981 agtacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc
    41041 agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac
    41101 gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat
    41161 cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg
    41221 gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc
    41281 tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact
    41341 gaagaagccc gcagtgacca gcggcagtgc cgacagtgct gcgccgccca ccaggaagca
    41401 gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg
    41461 atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg
    41521 agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta
    41581 gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc
    41641 ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc
    41701 cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat
    41761 cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca
    41821 tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc
    41881 ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg
    41941 gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc
    42001 acccacacgg gtcacgacga acgctttcat tgctgctgcg ccattcttcg gatcggtgta
    42061 atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag
    42121 cagcaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc
    42181 gaagaagcga gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag
    42241 gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt
    42301 aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgcctg
    42361 ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt acctgcgccg ccaggcccac
    42421 agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag
    42481 caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc
    42541 tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca
    42601 aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg
    42661 tcggtctgac cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg
    42721 atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca
    42781 agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct
    42841 ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt
    42901 cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca
    42961 ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac
    43021 cgtcgatacc ctgatcgtta acaccgagga tggcgtaaac aatcaccacc agcatgatgg
    43081 ccgacaaaat tgccgggcca atccacacct gcggtttcag ccactggcgt tcctgttcga
    43141 tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac
    43201 ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcctagtgag aagaacaccc
    43261 cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg
    43321 tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca
    43381 ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc
    43441 ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg
    43501 gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat cctggcgctt
    43561 gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc
    43621 gcacagacca cagaaaatgc agcgtgagaa gttaatgcgg aaaaattccg gataccagcg
    43681 accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcaca
    43741 gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc
    43801 acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc
    43861 gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa
    43921 ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc
    43981 gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc
    44041 tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac
    44101 gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag
    44161 aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac
    44221 agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct
    44281 tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc aaaggtaata
    44341 aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac gatgtcggtc
    44401 atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg
    44461 tagctcaggg tctgcgcaga agcacgcatc gcacccagca gcgagtattt gttgttactt
    44521 gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa
    44581 atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat tgcaaaggcc
    44641 agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat
    44701 ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa
    44761 ccgccccagc caacacggtt aggtccataa cggttctgga acagacccag caggcgacgt
    44821 tcgccaaagc tcatgaatgc cccacaggtg acaaccacca gcaggatccc caccgctttg
    44881 aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct
    44941 tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata cccatcggca
    45001 agcccacctg ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg accgtattgc
    45061 catcgtaact aaaggagacg cgtgtacctg cgttcacacc caacttcgcg gcatccgctg
    45121 ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca
    45181 attcatcgct gccaaacagg tggtagtacg gcgcgatacg ccatttcccg tcctgcggct
    45241 ggaagcgtgc cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt tcaaacagac
    45301 gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg
    45361 cctgcgggga gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg
    45421 gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg
    45481 gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac
    45541 gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacccg
    45601 ccagttccgg gattttcgcc acaacagcgt caattacatg gtcgagctgc gtccagtcca
    45661 cttcacggct cagcaaggtg ctgtgcagcg agtgcagcca gcgccagctt tccagcatga
    45721 cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt
    45781 cattattaat taccgtaccg tcgctttcag caaagctggc agcggaaagc accagatggg
    45841 cgttttccat gatcgctgtg cgttgatgat caaccaccat caccagcggc gctttagcca
    45901 gcgcagcatt cacgcgggta gcagaagcgt aacgatgcag atcgttttcc aacaccacca
    45961 ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca
    46021 taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgatacca acgtcagcgc
    46081 cgcgaccttt cagggctttc gcgacgttag ccgcagcctg aatcacctct atgctaccgg
    46141 cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt
    46201 cgattttgtt ttgcagctca ggttcgatac cgtcaaccgc tggtgcggag ttatccagcg
    46261 catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg
    46321 ccgcgatatc atccagacga gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac
    46381 gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgcagcca
    46441 tttcgcgcgc tttacctttc accgcctgac gcactgccag tgcgacgcgc gcgccggtct
    46501 gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg
    46561 gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac
    46621 cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc
    46681 tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat
    46741 ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct
    46801 gtactggctg acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt
    46861 cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc
    46921 cgtaacgttc accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg
    46981 caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc
    47041 cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg
    47101 ggcgaccgaa gtagacgttg tcgtgcgcgc cataaacgcc cagatctgta ccgtcagcgt
    47161 aatctttgta gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga
    47221 tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc
    47281 tgtgtccggt catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg
    47341 gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg
    47401 cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca
    47461 ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag
    47521 caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca
    47581 gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa
    47641 ttgtagccat tagcatgctt ccagttttct cggtcaggga ccgagcgtta atcgaaattc
    47701 ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg
    47761 aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac
    47821 ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac
    47881 agttgctcaa gtgtttcgat atcgcccggc tggccttcgc cacgctccag cgcacgcaga
    47941 attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca ggactcacgg
    48001 gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc
    48061 atcgccagcg ccgtacccag acggctgccc gctttaccga tactttcgaa ttccatcggc
    48121 agatcaaggt gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta
    48181 aatttcagac catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg
    48241 ccgaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg
    48301 gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc
    48361 gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg
    48421 gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg
    48481 attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg
    48541 aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca
    48601 atagcgcggc gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca
    48661 cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt
    48721 tgctccatca acaggcggtc tttataggtg cccggctcca tttcatcggc gttacacagc
    48781 aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccacttcag gccagtcgaa
    48841 aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc
    48901 ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg
    48961 tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga
    49021 gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca
    49081 gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc
    49141 agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca
    49201 gctttttctc gagcgccgcc tgaataccct gatacccgtt gatatgacag accacgctgt
    49261 cgcaataacg gatcacatgg cgaccaaccg gctggcggaa gatctgactg tagaacgttg
    49321 ccacaccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac
    49381 catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac
    49441 gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct
    49501 caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat
    49561 aacaaaatcg atactgccca gataaacaat caggtcagac accaggctgc cgcggatcgc
    49621 cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat
    49681 ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg
    49741 gaaagattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag
    49801 ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc
    49861 tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg
    49921 cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg
    49981 gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc
    50041 gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc
    50101 ataggcggca acgccctggg tacgaccttt cagaatggta ttttgcagcg ccgctttctc
    50161 gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcagac gatcccagcc
    50221 gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc
    50281 cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg
    50341 cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg
    50401 gaacagttcg gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc
    50461 cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat
    50521 acggtcggta tacggaatgt agctgtgcca ggactggcgc tcgcccattt tctccgcacc
    50581 acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa
    50641 aacgatacgg aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa
    50701 gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc
    50761 catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc
    50821 cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag
    50881 gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt
    50941 agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac
    51001 cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc
    51061 cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa
    51121 cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc
    51181 acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg
    51241 cccaaaacgg ttgcgcagtt cgccaattac cggatcatca agatgatcgc gggtctgcca
    51301 ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg
    51361 actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt
    51421 gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc
    51481 accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac
    51541 cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg
    51601 catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt
    51661 atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcccc
    51721 cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc
    51781 cattttggta aagcaggttc ctgcaaccac catcaggtca gcctgacgcg gcgaagcacg
    51841 caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc
    51901 aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt
    51961 aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc
    52021 ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg
    52081 ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt
    52141 cgtttccggg ttcatacact cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat
    52201 acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa
    52261 gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac
    52321 gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc
    52381 ggagccgacc gaatcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg
    52441 accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag
    52501 aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc
    52561 ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga
    52621 ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa
    52681 ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca
    52741 cattagttta cataatatca acaccattag catttaatgc cctttcaccc cagatccttg
    52801 acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat
    52861 acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt
    52921 gatctgtgac acgcttttgt tattccataa caaaaacgca gcaacaaatt tacgtatttt
    52981 ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa
    53041 tctctggtga ttgaaaaata aacagttttg tacgttctgc actatgcgag gaaggatttt
    53101 tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg
    53161 tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc
    53221 atccccttcc ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc
    53281 ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc
    53341 ggtatcaggt agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat
    53401 catctcaacc ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg
    53461 aagcgtcgag gcgacataag ccagtcgcca tggaatatct gctttattaa gcgtcgccag
    53521 caccatatcg cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt
    53581 ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc
    53641 cttaaacgct gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat
    53701 ttctgccata taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga
    53761 actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat
    53821 agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg
    53881 caggattttc ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg
    53941 accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact
    54001 tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc
    54061 aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact
    54121 tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca
    54181 aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa
    54241 gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg
    54301 aagtagatca cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc
    54361 tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg
    54421 cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaagcgg catcaagagc
    54481 agggatccgt catttatgcg agcattttac cccaaaacct tttatttata agggtcattg
    54541 cgaattatct gatgcaaagt tatgttatgt tatgttatgt tatgttatgt tatgttatgt
    54601 tatgttaggc aaagtaatct tctattatta ataagcacat caaaaccttt ttgaatatta
    54661 aataataatt aattagcatc atcctcattc attaattccg cttaacaata gtttcacacc
    54721 ttatccccat tgcgacacct cggaaatcat cacgctgtta aactctccgt aatgcggaaa
    54781 tatcatcatc gcgtttcatt agtgaattct taaatgaggc attttcacac aattatctta
    54841 cagataaaaa aaccagactt gcaattaaga attagaacaa ttcactatat aacattacat
    54901 gtaaagcata tacacctcat tattttgtca ttattaagtt attaacagca caatcgagcc
    54961 ttcccctctg gcaaaatctt attctgcaga ccttcaaaac accgtcctgg gggagtacat
    55021 tgttctaagc tgacttccac ggcagggagt ggcgataaca gcaaaaaagg tcaagattca
    55081 tgtcccccat tgaaaaatcc agcaaattag agaatgtctg ttatgacatc cgtggtccgg
    55141 tgctgaaaga agcaaaacgc ctggaagaag aaggtaacaa ggtactgaaa ctgaacatcg
    55201 gcaacccagc cccattcggt tttgacgcgc cagatgaaat cctcgttgac gtgatacgca
    55261 acctgcctac cgctcaaggg tattgcgatt ccaaagggct ttactccgcg cgtaaagcca
    55321 tcatgcagca ctaccaggct cgtggcatgc gtgatgttac cgtggaagat atttacatcg
    55381 gcaatggtgt atcggagctt atcgttcagg caatgcaggc attgctgaac agcggggacg
    55441 aaatgttggt tcctgcacca gattacccac tctggaccgc ggcggtttcg ctttccagcg
    55501 gtaaagcggt gcattatctt tgcgatgaat cctctgactg gttcccggac ctcgatgata
    55561 ttcgcgctaa aattacgcct cgtacgcgtg ggattgttat tatcaaccca aataacccaa
    55621 ccggcgcggt atattccaaa gagcttttaa tggagattgt ggagattgca cgtcagcata
    55681 atctcattat cttcgccgat gaaatttatg acaaaattct ctacgacgat gctgagcatc
    55741 actcaattgc gccgctggca cctgacctgc tgaccattac ctttaacgga ctgtcgaaaa
    55801 cgtaccgcgt tgcaggcttc cgtcaggggt ggatggtgtt gaacgggccg aaaaaacacg
    55861 ccaaaggcta catcgaaggt ctggaaatgc tggcttcaat gcgcctgtgt gctaacgttc
    55921 ctgcgcaaca cgccattcag accgcgctgg gtggttatca gagcatcagt gaatttatta
    55981 cccctggcgg tcgtctttat gagcagcgta accgcgcgtg ggaactgatc aacgatattc
    56041 cgggcgtttc ctgcgtgaaa cctcgtggtg cgctgtatat gttcccgaaa atcgacgcca
    56101 aacgctttaa cattcatgac gatcagaaaa tggtgctgga tttcctgttg caggaaaaag
    56161 ttctgttggt gcaagggacg gcattcaact ggccgtggcc ggatcacttc cgcattgtca
    56221 cgctaccgcg tgtcgatgat atcgagctgt ctttgagcaa gttcgcgcgt ttcctttctg
    56281 gttatcatca gctgtaatct taatttcact gccggagatt gcatccggca gtgttatccc
    56341 gccacaatga cctgatgatg tcatcatacg taaggtcact atgaaacaga gccatttttt
    56401 tgcccatctc tcccgcctga aactcattaa ccgctggccg ctaatgcgca acgtgcggac
    56461 ggaaaatgtg tccgaacaca gtttgcaggt agcgatggtc gcccatgcgc tggcagctat
    56521 caaaaatcgg aaatttggcg gtaatgtcaa cgccgaacgt atcgctttac tggcgatgta
    56581 ccacgatgcc tcagaagtgc tcaccggcga tctccctact ccggtgaaat acttcaatgc
    56641 gcaaatcgct caggagtaca aggctattga aaaaatcgct cagcagaaac tggtcgatat
    56701 ggttccggaa gaactgcggg atatctttgc gccgttaatt gacgagcatg catatagcga
    56761 tgaagaaaaa tcgctggtga aacaggcaga tgcactgtgt gcatatctga aatgtctgga
    56821 agaactcgcg gccggaaata atgaattctt gctggcaaaa acgcgactgg aagcgacgct
    56881 tgaagcgcgt cgcagccagg agatggacta cttcatggaa gtatttgttc ccagcttcca
    56941 tctttcgctc gatgagatta gccaggattc accgctgtaa gcagccggag tctgcgtcgc
    57001 atcaggcaat aagcgccgga tgcgacatca ggctcttgtc aaaacggaaa cagcatcggg
    57061 atcatcacca cacaaaccgc catcacgata atggtgaacg gtacccccaa cttcacaaag
    57121 tcactgaagc tgtaatttcc cggacctaaa accagggtgt taacaggtga agacaccggt
    57181 gtcataaagg cggcggatgc tgccatcgcc acgaccatcg caaatggata aggcgacact
    57241 cccatcgttt tggcagcagc cagcgcaatc ggagccatca acaccgccgt cgcggtatta
    57301 gagataaaca gcccaataac cgccgacaag acaaacaaac accccagcat catatgtggc
    57361 ccataaccgc cgccaatatc catcagccct ttcaccgcca gcgcgacacc tcccgttttc
    57421 tgtaatgcca cagcaaacgg catcatccca acgatcaaaa taatgctcgg ccagtgaatg
    57481 gatttatagg cgctttcagc atctatacag cggaatttcc ccatcagcag gcaggcgatg
    57541 atagcggcaa caggattagg aatttcattt gtcagcatta acgccaccat tagcaccaga
    57601 cagaaaatgg catggggtgc ctggctgtgc gcgggcgatg cttcactcac ctcttccggt
    57661 aagttcagcg ctacgaagtc gcggccctgt ttggccagca taccgatcag tttccagtta
    57721 cccacaacca ggatgatatc gcccagcagc agaggctcat ccgccagcga accttccagc
    57781 gccacgccat tgcgcttgag ccccaccaca ttcagtccgt agcgggtacg aaaaccaatt
    57841 tcgcgcaccg atttaccaat cagttctgac tcaggaatta atgaaatctc tgccatgccc
    57901 acatcaaggg cctggtcaga aaaatactcg ccgcgcagta ccatcggctc cagcaattgc
    57961 tcactacaaa attgccggag atcgacatca gccgcagaca tatcaataag caaaacgtca
    58021 cgcgcgcgaa attcagaaac cccattaacg ttcacgataa cgcgacgaaa acgccgccag
    58081 cgttcaacac cgatgacgtt agcgccataa cgctcacgta atttgagatc atccagccgt
    58141 tgacctatca ttggcgatcc ggggcgaata gccagacgtc gcgcacgccc ggtcagtcga
    58201 tattcacgga taagatcgcg aaaggttcga cgcgtccagc cttcgcgctg cggggtctgg
    58261 gtatcccctt tcagcatgaa acgcatcact aacatataca aaatacccag caccagcacg
    58321 accaggccaa taggtgttac gctaaagaaa ctgaagccgt gatagccttc acgcagcaat
    58381 tcactgttga ctaccaggtt cggcggcgtc gccaccagcg tcatcatgcc gctaatcagc
    58441 ccggcaaaac ttaacggcat catcagacgt gacggcgaag tttgcatacg catggcaacg
    58501 cttaacacca cgggaataaa gatagcgaca acgctggttg aactcataaa cgcgccaagc
    58561 cccgcgacgg tcagcatcaa caaaaccaac attttgattt cactgttgcc cgccactttg
    58621 accagccatg ttcccattac ggtggcaaca ccggtacgga ccaaaccatc gccaataata
    58681 aacaaggcgg caatcaggac aacgttagga tcagaaaagc cggaaaatac ttcggggacc
    58741 gtcagcgttc cgcttaatgc aaacgcgaca ataacaaaca aagcgaccgc atccatacgc
    58801 actctgcccg tcgcaaacaa gacgatggca acggccagta atgaaagaac ccaaatcaat
    58861 tcaccgttca caacttatcc ttgttaattg agggggatga cttgattctg ccataaaaaa
    58921 gccccgacga gacggggcta aatcatgatc aggtgtttca ctgaataata acatcgccat
    58981 ttggctgttt ggtcacagta atttgctcca gactgtggag gaccaaatcg acctcattca
    59041 ggcgcggggt atctgccgga gcgttaaccg caatgacatg gcaacccgcc gctaggccag
    59101 aaagcacgcc agcgggagca tcttccacca ccacacactc ctgcggcgca agccccagca
    59161 gctgcgcacc taacagatac gcatcaggtt ctggttttcc gcgcttcact cgctcagcgg
    59221 ttacaaacac ctctggtgcg ggaagccccg ctattttatg gcgcgctcgc gctaccggca
    59281 tggagccaga agtcacaatg gcccacggaa tacctgcttt attcaaatga ttgagtaagg
    59341 cgattgcccc agaaagcgcg gtaatacctt cggtttccgt ggcctcgatt tgctccagac
    59401 gcgtaaactc ggcggcaata tcagcctcgg atttgcccgc cataaaatgg cgcaaagagg
    59461 tgatcgcctg tttaccgtga atgaaagcca gcacctcttc cggcgctaac ccatgacgtc
    59521 tggcccagtt gctccacgcc cgttctaccg caggcaggga atccaccagc gttccatcaa
    59581 gatcaaacag aaaacctttg caccgcacgc gggcctcctc aggcgttgat aatttgatta
    59641 atttcgttgg cgctcaaatg gtactgacgc gggcaggcat gccacacatt aagcatgcgc
    59701 tgatattttt cccacattgg cgtctgggcg ttaaaaccat gagtcccagc gtcaaaatgg
    59761 gtatagcgcc cttccacatt aaccataaag cggacataac cgaggtaacg tgcttcagtg
    59821 gcggcgtcaa agccgaggaa ggtgacacga cgttcatcga tggattgctg gtcctgcaaa
    59881 ttagaccagg aaacatgcaa cgcatgatac atctccataa tgtcgatgat agtgcggcag
    59941 gtttcttctt tcagctcgcc aaactcgcga tccaattcac gcatctgtaa tccgtaacca
    60001 cgctcaataa ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc gagcatagtc
    60061 atcatcttgt actgattaga caaaataaga cgttgcgcgt tggtcatttc cattgttgac
    60121 tcctgtatca ctctactacg gtgaaaaaaa agaaggctga gtatgccttc ttttatatgc
    60181 gtaatcaggg gtcaattaca aatcatcaag gaaagtttta tccagttgtt tgaaggcgcg
    60241 cttaagcgtg tcagctaatg cctggtaatc aggcttgcct tcaacgggtg ccaacacctg
    60301 tccagactcc tgcaatttac cgcgaacttc ataaaaccag ttaaggattg cagggggtaa
    60361 tggcgttaca gaacgcttgc ccagccacca caatccctgc atgggtaaac ttaaggcgaa
    60421 cagtgcagtg gcaactgccg gcccaagcag accgcccagg gcaatctgcc agcagagagt
    60481 aaatacggcg atcggcggca taaaacggat cgcataacgc gtcatcttga taacgcgatt
    60541 ttcgacaaag accggggcaa ggcgtttttc cagtggccac gtctttgagt aatgctgtcc
    60601 gcggcgaaac aagctaaaaa aattaacaga acgattatcc ggcgttgaca tgcttcacct
    60661 caacttcaca tataaagatt caaaaatttg tgcaaattca caactcagcg ggacaacgtt
    60721 caaaacattt tgtcttccat acccactatc aggtatcctt tagcagcctg aaggcctaaa
    60781 tagtacatat tcattgagtc gtcaaattca tatacattat gccattggct gaaaattacg
    60841 caaaatggca tagactcaag atatttcttc catcatgcaa aaaaaatttg cagtgcatga
    60901 tgttaatcat aaatgtcggt gtcatcatgc gctacgctct atggctccct gacgtttttt
    60961 tagccacgta tcaattatag gtacttccat gtcgagtaag ttagtactgg ttctgaactg
    61021 cggtagttct tcactgaaat ttgccatcat cgatgcagta aatggtgaag agtacctttc
    61081 tggtttagcc gaatgtttcc acctgcccga agcacgtatc aaatggaaaa tggacggcaa
    61141 taaacaggaa gcggctttag gtgcaggcgc cgctcacagc gaagcgctca actttatcgt
    61201 taatactatt ctggcacaaa aaccagaact gtctgcgcag ctgactgcta tcggtcaccg
    61261 tatcgtacac ggcggcgaaa agtataccag ctccgtagtg atcgatgagt ctgttattca
    61321 gggtatcaaa gatgcagctt cttttgcacc gctgcacaac ccggctcacc tgatcggtat
    61381 cgaagaagct ctgaaatctt tcccacagct gaaagacaaa aacgttgctg tatttgacac
    61441 cgcgttccac cagactatgc cggaagagtc ttacctctac gccctgccgt acaacctgta
    61501 caaagagcac ggcatccgtc gttacggcgc gcacggcacc agccacttct atgtaaccca
    61561 ggaagcggca aaaatgctga acaaaccggt agaagaactg aacatcatca cctgccacct
    61621 gggcaacggt ggttccgttt ctgctatccg caacggtaaa tgcgttgaca cctctatggg
    61681 tctgaccccg ctggaaggtc tggtcatggg tacccgttct ggtgatatcg atccggcgat
    61741 catcttccac ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca aactgctgac
    61801 caaagagtct ggcctgctgg gtctgaccga agtgaccagc gactgccgct atgttgaaga
    61861 caactacgcg acgaaagaag acgcgaagcg cgcaatggac gtttactgcc accgcctggc
    61921 gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg ttgtattcac
    61981 tggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca aactgggcgt
    62041 gctgggcttt gaagttgatc atgaacgcaa cctggctgca cgtttcggca aatctggttt
    62101 catcaacaaa gaaggtaccc gtcctgcggt ggttatccca accaacgaag aactggttat
    62161 cgcgcaagac gcgagccgcc tgactgcctg atttcacacc gccagctcag ctggcggtgc
    62221 tgttttgtaa cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc ccgtattatt
    62281 atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg cgtgatccgt
    62341 gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca gccgcgtacc
    62401 ggtggcgatg cgcccgatca gactacgact atcgtgcgtg cgaactcttc caccacgacg
    62461 gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa tcagaaagat
    62521 gtgctgatgg aagagatcgt cgcaaactac cacgctaaca ccaaagacgc tgaagtcgtt
    62581 ctggttgaag gtctggtccc gacacgtaag caccagtttg cccagtctct gaactacgaa
    62641 atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac tgacaccccg
    62701 gaacagctga aagagcgtat cgaactgacc cgcaacagct tcggtggtgc caaaaacacc
    62761 aacatcaccg gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca gggtcgtact
    62821 cgcccggatc tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa caatgttgat
    62881 ccggcgaagc tgcaagaatc cagcccgctg ccggttctcg gcgctgtgcc gtggagcttt
    62941 gacctgatcg cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac catcatcaac
    63001 gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg cagcattccg
    63061 cacatgctgg agcacttccg tgccggttct ctgctggtga cttccgcaga ccgtcctgac
    63121 gtgctggtgg ccgcttgcct ggcagccatg aacggcgtag aaatcggtgc cctgctgctg
    63181 actggcggct acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc tttcgctacc
    63241 ggcctgccgg tatttatggt gaacaccaac acctggcaga cctctctgag cctgcagagc
    63301 ttcaacctgg aagttccggt tgacgatcac gaacgtatcg agaaagttca ggaatacgtt
    63361 gctaactaca tcaacgctga ctggatcgaa tctctgactg ccacttctga gcgcagccgt
    63421 cgtctgtctc cgccagcgtt ccgttatcag ctgactgaac tggcgcgcaa agcgggcaaa
    63481 cgtatcgttc tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc tatctgtgct
    63541 gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa ccgtgttgca
    63601 gcgtctcagg gtgtagaact aggtgcaggc attgaaatcg ttgatccaga agtggttcgc
    63661 gaaagctatg ttggtcgtct ggtcgagctg cgtaagaaca aaggcatgac cgaaaccgtt
    63721 gcccgcgaac agctggaaga caacgtggtg ctcggtacgc tgatgctgga acaggatgaa
    63781 gttgatggtc tggtttccgg tgctgttcac actaccgcaa acaccatccg tccgccgctg
    63841 cagctgatca aaactgcacc gggcagctcc ctggtatctt ccgtgttctt catgctgctg
    63901 ccggaacagg tttacgttta cggtgactgt gcgatcaacc cggatccgac cgctgaacag
    63961 ctggcagaaa tcgcgattca gtccgctgat tccgctgcgg ccttcggtat cgaaccgcgc
    64021 gttgctatgc tctcctactc caccggtact tctggtgcag gtagcgacgt agaaaaagtt
    64081 cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga cggtccgctg
    64141 cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca aagcgccgaa ctctccggtt
    64201 gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca ccggtaacac cacctacaaa
    64261 gcggtacagc gttctgccga cctgatctcc atcgggccga tgctgcaggg tatgcgcaaa
    64321 ccggttaacg acctgtcccg tggcgcactg gttgacgata tcgtctacac catcgcgctg
    64381 actgcgattc agtctgcaca gcagcagtaa tctcgtcatc atccacagct ttgcgctgcg
    64441 gatatctgaa ccggaaataa tcactatttc cggtttttta ttctcttaat ttgcattaat
    64501 cctttctgat tatcttgctt aactgcgctg catcaatgaa ttgcgccatc tcactttgca
    64561 tacttaccac tttgttttgt gcaagggaat atttgcgcta tgtccgcaat cactgaatcc
    64621 aaaccaacaa gaagatgggc aatgcccgat acgttggtga ttatcttttt tgttgctatt
    64681 ttaaccagtc ttgccacctg ggtagttccg gtggggatgt ttgacagtca ggaagtgcag
    64741 tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag atccacactc atttcgcatt
    64801 ctgactaacg aagcaggcga acctgagtat caccgcgtac agctgttcac gacgggcgat
    64861 gaacgcccgg gcctgatgaa cttcccgttt gaagggttaa cctcaggatc gaaatacggg
    64921 acagccgttg gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg cattgtgatg
    64981 cgtacaggaa ccattgataa cggtatcctg gcgcttattc gccatacccg cgggaatgaa
    65041 attctcttta ttcctgcgct gtttattctg ttttcacttg gcggcgcggt atttggtatg
    65101 ggagaagagg ccgtcgcctt tgccattatc atcgcaccgc taatggtccg gctgggctat
    65161 gacagtatta ccaccgtcct ggtgacttat attgccacgc aaatcggttt tgccagttcg
    65221 tggatgaacc cgttttgtgt ggttgttgct caggggattg ccggcgttcc ggtgctttct
    65281 ggctccgggt tgcgcatcgt ggtgtgggtt atcgccactc tgattggcct gatctttacc
    65341 atggtgtacg cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca tgagtccgac
    65401 cgcttctttc gtgaaaagca ggcggatgtt gaacaacgtc cgtttacctt tggtgactgg
    65461 ctggtattga ttgtcctgac agccgtaatg gtctgggtga tttggggcgt gatcgttaat
    65521 gcctggttta ttccagaaat tgccagccag ttcttcacca tgggtctggt gattggcatc
    65581 atcggcgttg ttttccgcct taacggcatg acggttaata ccatggcttc atcctttacc
    65641 gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa agggattttg
    65701 ctgctggttg gtaatggtga agcaggtaat gccagcgtgt taaatactat cctcaacagc
    65761 attgccaatg ccattagcgg tctggacaac gcggtcgcgg cctggtttat gttgctcttc
    65821 caggctgtat ttaatttctt cgtgacgtcc ggttctggtc aggcggcatt aactatgccg
    65881 ttactggcac cgcttggcga tctggtcggt gttaaccgtc aggttaccgt gctggcgttc
    65941 cagtttggtg atggcttcag ccacatcatt tacccaacct cagcttcgtt aatggcaacg
    66001 ctcggtgtgt gccgggtgga cttccgtaac tggctgaagg tgggcgcgac cctgcttgga
    66061 ctgctgttta ttatgtccag cgtcgtagtg atcggcgctc agttgatggg ctaccactaa
    66121 aaatgttaag agccgcaatt gcggctcttt tttcattctg ccgtttcagt ctctacggct
    66181 tcatttttgg cattgcgctt catccacaac gccagcgctt tcagcgagtc tggagtgaac
    66241 tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc aaacttcact gacttcatct
    66301 tcctgtaggg cgaagggacc gtgagagacg caactgaaca acgcgcccca gacacggcaa
    66361 tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc aatgcccaac
    66421 tcttcttccg cttcgcgacg cgcggattcc agcagttgct catcggcctg aactacaccg
    66481 cctgcggtcg catctaacat gccgggtaaa aagtcttttg tctcggtacg acgctggacc
    66541 agaattttgc ccatgccatc atgcacgacg atgtaagttg cacgatgacg cagacactgt
    66601 gcccgcattt gttcccggct ggcttgtgca atgacttcgt tctcttcatt gacaatatcc
    66661 acccattcag tacttgccaa acgacgctgt tccattatca ggaaaccttc tttttctggc
    66721 gctcttacgg cgcattttga gttgtgggta aattacggat taatcgcgac ctgcgcaatg
    66781 atactttgat cattgagtgc gattacacta agtacgtcat tatccagcat gccataactc
    66841 gccggattac cgcctttcgg aatactcacc gagccggggt tgaagtggaa aatctccccc
    66901 cgctgttctg ccactggcag atgggtatga ccgtacacca gcacatcgtt ctggtttaaa
    66961 gcaggtagat tttccgggcc aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc
    67021 agtaatacct gttgccacgg cgcggttatc gggaaatgca gcatcatttg atccacttcg
    67081 ctgtcgcagt tgccacgcac agcgataacc ttatgtgcca cttcattaag ccgttcagcg
    67141 actttggctg gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt caacacgtcg
    67201 ccaaggatca ccagccactg ggcaccgctt tggacaaaca actccacaac acgttccgtc
    67261 gccggtaacg acccatgaat gtccgatgca aacatcagtt tcatcactca ctcctcgtcg
    67321 aaaaaacgta agctatgata ctggattctg tcgttgctat cagccagatt gcttcgccga
    67381 aagtgcaata aaacgctgga cagacgctcg ctgccactgg aatgtcgcat aatccaccag
    67441 gcgttctggc acctcatcgc catgtagtac caggcggtta atcattagcg ccagatcagt
    67501 atcagcaatg caccattcac caaataaatt cggctgaccc agtgctaaca aatgttctgc
    67561 catcgcgaac agtttctctg cactggcttt tccctcggcc gttagtggcg cttttttcgc
    67621 ccccgcaaag acaacatccg tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag
    67681 ccaggcctga atctgtcgtg cacgcgcacg attttctaaa tcaagcggat aaatacgttc
    67741 ccaggtcggt ggcgcaaatc gatcttccag atactccgca atggcagaag attcactcaa
    67801 ctcaaaatca tcgatttgta ataacggcac acggcgtgtc tgaccgtaac cttgccacgt
    67861 cggctgcaaa tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg acaggccttt
    67921 ttcctgcaac gccacccagg cggataacac ataaggggag aaaaagtggg catctgacca
    67981 aagcgtgata gcgggtttac tcattatgtc ctcggcaata aagggggctg gctatcaaac
    68041 tatagagcct ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta
    68101 tacttgtggt gttcagatga ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc
    68161 ccgacaccca atggtcacaa aattacgctg tttctcgaag aagcagagct tgattatcgc
    68221 ttgattaagg tagacctggg gaaaggcggt cagtttcgtc cggaattttt gcgcatttcg
    68281 cccaacaaca aaattccggc aattgttgat cattctcctg ccgatggcgg cgaaccgtta
    68341 agcctctttg agtctggtgc cattttgttg tatctggctg agaaaacagg actctttttg
    68401 agtcatgaaa cgcgtgagcg cgccgccaca ttacagtggt tattctggca ggtaggcgga
    68461 ctggggccga tgcttgggca aaatcatcat tttaatcacg cagcccccca aaccattcct
    68521 tacgctattg aacgttatca ggttgaaact cagcgtcttt accatgtact aaacaagcgg
    68581 ctggaaaact cgccctggct gggaggcgag aactacagca ttgcggatat tgcctgctgg
    68641 ccgtgggtta atgcctggac tcgcctgcga attgacctcg caatgtatcc ggcagtcaag
    68701 aactggcatg agcggatccg ttcgcgccct gccaccgggc aggcactgct aaaagcacaa
    68761 ctcggtgatg agcgttcgga tagttaacag aaacaggttc tcgtgtatta tttcatccta
    68821 agtaaaacaa cggagaacct gcaatggcac aacctgccgc tattattcgt ataaagaacc
    68881 ttcgtttgcg tacgtttatc ggaattaagg aagaagaaat taacaaccgt caggatattg
    68941 ttatcaatgt gacgatccac taccccgccg ataaagcgcg cactagcgaa gatatcaacg
    69001 atgcgctgaa ttatcgcacc gtaacgaaaa acattattca gcatgtagag aataaccgtt
    69061 tctctttgct ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa catcactggg
    69121 tgacgtatgc tgaagtggag atcgataaac tgcacgcgct gcgctacgcc gattcggtat
    69181 ccatgacctt aagctggcag cgttaatcgc catatcggga ggctgcatga acatagtgat
    69241 caccggaggg acgggattaa ttggtcgcca tttgattccg cgtttgctgg agctgggcca
    69301 tcaaattacg gtagtgacgc gtaacccgca gaaagccagt tccgttctcg gccctcgggt
    69361 gacactatgg caagggcttg ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa
    69421 cctggccgga gaaccgattg ctgataaacg ctggactcac aagcaaaaag agcgtctctg
    69481 ccaaagccac tggaatatca cgcaaaaact ggtcgatttg attaatgcca gcgacacgcc
    69541 gccgtcggta ctcatttccg gctcggcaac gggctattat ggcgacttag gtgaagtggt
    69601 ggttaccgaa gaggaaccgc cgcataacga atttactcat aaactctgcg cccgctggga
    69661 agagattgcc tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc gaaccggcgt
    69721 ggtactggca ccggacggcg gtattctcgg taaaatgctg ccaccgtttc gtcttggcct
    69781 gggcgggccg attggttccg gtcggcagta tctggcctgg attcatattg atgatatggt
    69841 caacggcatt ctctggctgc tggataacga gctgcgcggg ccatttaata tggtttcgcc
    69901 ctacccggta cgcaatgaac aatttgccca tgcgctcggt catgcgctgc atcgcccggc
    69961 tattttgcgc gtccccacaa ccgccattcg gctgttaatg ggcgaatctt cagtactggt
    70021 attaggtgga caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg
    70081 gtacgattta gaagaggcgc tggcggatgt cgttcgctga tgtggtttac agcaaacatc
    70141 cgccagttaa ctccctgtgt tacaggatta gtggctttgc gcgataagat cgtctggtga
    70201 aagtcgggtc accatcataa ctaactctct gtctaaacct ctatccagca tctcctgagc
    70261 aatacgcagg gcttcttcgt gtttgccctg cattgcgcct tcttcacgta atctgtcagc
    70321 aatggtcatc agtttctcct tttcttgtgg tgcgcgttct gttatctcac caataaatgc
    70381 acggaaacgc tgggcatccc ctgtttgtaa tacgtaatta aacagggctt ttagctgtct
    70441 gtcattagtg ttccctgtaa ctagcagcga aacaatttgg tcgactaatc ccaacagatc
    70501 gcgctgacga atatgtttct gaattaactc caacagcgcc attttgcggt gttgcataat
    70561 ctcgtcatcc ggcaccacgg caatatccac caacggaaac gctgatgaat atattttgcg
    70621 ggctatagca ggctcggcaa attcatcaag ccagcagagt gaataaggat aaggacttct
    70681 gcaaccatga taaaacagca tcgggatcac caatggaagc tctttatagc ccgcatcaag
    70741 atggttttgc attgccgcaa tggaataacg catcatgcga aaagccatta attcttccgg
    70801 cttactttgg tgctctatca ctacataaat ataacccact ccctcctgcg ttttcacaga
    70861 ccacaagagg tcggaataat attgccgcag gtcttcatca ataaaactgt ttggttctag
    70921 tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg ggaagatgaa tattaataaa
    70981 attccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat gcggcgtgga
    71041 agttgtcgat attgtcatgg cgttccgtca cccctcaaat ttaagatgac gcgacaataa
    71101 ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa ttcagaagta
    71161 tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat tccccttgaa
    71221 aatactccgg cagcgttagc caccggagag gaaattattt cagcgatccc ttaaggaact
    71281 gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgccc tcttcttcta
    71341 ttttcccctg atggaggaaa atgacgtgag tagaaacatg gcgagcaaag cccatttcgt
    71401 gagtcactac caccatggtt ttcccctctt ctgccagttg ctgcataata cgtaacactt
    71461 cgcctaccag ttcaggatcg agcgccgagg taggttcatc aaacagtaaa acttctggtt
    71521 ccatcgccag cgcccgcgcg atagaaacac gctgttgctg accgccggaa agatgcaccg
    71581 gatatttccc ctgcgcacgt tcgtctatcc cgactttcgc cagatacttc accgcccgct
    71641 cgcacgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg acgttttcca
    71701 acaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg cgtgtgcgca
    71761 gtaagcgcag ttgattttta tcggcgactt tgagttgacc gtctttgtcg cgcaccagat
    71821 tgatcgtctg gccattgacc acgatcgatc cttcactcgg tttttcgagg aagttaatgc
    71881 agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatt acatctccgg
    71941 cattcgcttg cagtgagacc cctttcagca cttcgtgttc gccatagcgt ttgtgcaaat
    72001 cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga aggtttcaca
    72061 tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata agagatgatt
    72121 aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc ggcgttaata
    72181 tcgcgggcta ttttcagcag atccggcaca gtggccgtaa acgccagcgc agtagagtgc
    72241 agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc agaaggcaaa
    72301 ataatgcagc gatacatttt aaaggtcgag aagccatagg ctctggcggc ttcaatttcc
    72361 ccatgcggaa ccgaacggat tgccccagca aaaatctcgg tagtgtaagc gcaggtgtta
    72421 agcgtcagcg ccagcacggt acagttcagg ccactgcgga agaaagcgtt aaggaattcg
    72481 gttcccttaa caatctcaag cgtgtacatg ccggaataga acaccagcaa ctgaacatac
    72541 agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat gtatttatta
    72601 ctggagacac gaccaatcgc cagaaacagc gccaggactc cgcctatcac taccgacaaa
    72661 ataagcagcc acagagtgat cgccacacca gtaaagcgat aaccgtcggt ccacagcagc
    72721 ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca cccacggagt
    72781 agcggcgctc aaggaacagc agcacaccat tggaaacggt ggtgaaaacc aggtaaatca
    72841 cgccacagac gatggcgaaa tagaacggtt cccaggtact tttgcctgcc agctgcgtgg
    72901 ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg aggatcacct
    72961 gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac atgatcctcc
    73021 gaaacacttg cccgcgagta aaaccgaacg ccgtcgccgc ctctatatgc cctttcggca
    73081 ctgccataaa cgcgccacgg aacgtttcgg taaaataagc accgtagata aaaccgagag
    73141 tgataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc gcctccgtca
    73201 ccatgtttag cgcaatctgc aaaccataga aaatcagcag catcaacacc aagtccggca
    73261 cgcctcgaat cagcgtggtg tacccttcga aaatcagccc caaaagccga ttttgcgaga
    73321 gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagag ctgatagcca
    73381 gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatac aacatgctgc
    73441 ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac ccgaagggag
    73501 cgggctttct ttcaccgatg gcgattaacc accataaaca tcaaaatcga agtacttttt
    73561 cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt tcagtgcctc
    73621 gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa acagtttttc
    73681 atctttaaca gacggaccac cgaatttgta atctttaccg acgggttgtt tgaggaaacc
    73741 ttcgctggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag tcaggtcaga
    73801 ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg cccagtgttc
    73861 attaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc ctttcagcga
    73921 ctcgactgtc ggctgaatgt cagaattttt cgccaccacc agacgagaat cggcagcgta
    73981 cagtttgtcg gtgaaggcaa tttcttgctg acgtttttcc gtaatggaaa gcgatgacat
    74041 gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg ggttttcgac
    74101 aaacgtacat tgcgtattga tgcgcttgca caactccttc gccagctcga tatcgaaacc
    74161 aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt cggtaccgat
    74221 gcggatgttt tgcggaattg cagcaagtgc cgcagttgcg ctggagaagg ccagaaccag
    74281 agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt ttatagggaa
    74341 tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc aaaatatccc
    74401 gccccgttag acgtattatt tttcattaaa aatgcttaag tatgcatttc aatgcaccac
    74461 gatagtgcaa caaacctggc gtgcaccatg atgatcattc catcaggtac agcttcccag
    74521 cgacgtatca gtcaccgtag acattaaagt cgaaatactt tttcgccatc ttgtcgtagg
    74581 tgccgtcctg acgcagctcg ccaagcgcct tattgaaggc agccgtcagt tcagcatcat
    74641 ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttt actgatgagc
    74701 cagcaaaggc gaaatcttta ccagcaggtt gcttgaggaa tccttcgctg gcagcaactt
    74761 catcttgtaa cgcagcatcc agacgtcctg cagccagatc ggaatagacc aaatcctggt
    74821 tggcataggc caccacatcc acgcctttac tacgccaggt ctcgttagcg taagcttcct
    74881 gggttgatcc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc gttggctgaa
    74941 tcggtgaacc tttggcagca atcaaacgag aatctgcggc gtacagcttg tcggagaagg
    75001 caatctcctg ctgacgttta tcggtaatgg aaagcgacga aataatagcg tcgatttttt
    75061 tcgctttcag tgaggggagc agcgcgtcaa agtcactggc aacccaggta catttcacct
    75121 gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa tcacctttag
    75181 catctttcga cgagaacggc gcataggtgg tatcggttcc gatacgtacc gtctccggta
    75241 gcgccgcata gctggaagcc gctgcggaga gaccgactaa taaagacaga gcgagaatcg
    75301 acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta ttgtgttgtg
    75361 tgtttgcagg ctctttcatg caggtcttat gccatcttga cgacaacagt aacattcaac
    75421 gttaaatatg ttaataagac gttgcattat tgtcctgaag ttgaagatag caggtatagc
    75481 ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat ttgatcgcgg
    75541 ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca gcgggcaaag
    75601 agatcttcag gaagggttat tgcaaactgg tcaagaacac gattaaccgt ctgatttatc
    75661 acatcatcaa gggattgcgg gcgatgataa aacgccggaa cgggaggcat aatcaccgca
    75721 ccgatttctg ccgcctgagt cattaaacgc agatggccta agtgcaatgg tgtttcacgc
    75781 acgcagagca ccaacgggcg acgctctttc agcaccacat ctgccgcacg ggtcagtaag
    75841 ccatcagtat agctatggac aatgccggac agggttttga ttgaacaggg taaaatcacc
    75901 atccccagcg tctggaaaga accggaagag atgctggcgg aaatatcgcg cgcatcgagc
    75961 gtgacatcag ctaatgcctg cacttcgcgc agggaaaaat ccgtttcgag ggataaggtc
    76021 tggcgcgccg cctggctcat caccagatgc gtttcgatat ctgtgacatc gcgcagaacc
    76081 tgtaataagc gcacgccata aatcgcgccg ctggcaccgc tgatgcctac aatgagtcgt
    76141 ttcataaata gttgccctgt cagacttgca ggcagacttt gcagtatttc ggcgggagtt
    76201 gcaagtcagg gtgccagacc ggcaccctca gcgaaggcat catccttcgt tatgcatttc
    76261 gagattttcc acttcgttct gacgttgcac tgctttggcg tcatcattac gtaacgtatc
    76321 gaggaaatcg aggtagccct gatcaacatc tttggtgacg tagacgccgt tgaacaccga
    76381 gcattcaaac tgctggatat ccggattttc agcgcgaacg gcgtcgatca gatcgttcag
    76441 atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcaa cttcgcgacc
    76501 gtgagcgatc agttccgtgg cgctcggcat atcaatacca taaacgttcg ggaagcgaat
    76561 ttccggtgcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg ccatctcgat
    76621 aatctgctca gaagtggtgc cacggacgat ggagtcgtcg accagcagga cgtttttatc
    76681 gcggaactcg gcgcggttgg cattcagttt acggcgcacg gacttacgac gcagctgctg
    76741 gcccggcatg ataaaggtgc ggccaacata gcggttttta acgaagccct ggcggtacgg
    76801 tttgcccaga atacgagcaa tttccagcgc gatatcacac gaggtttctg ggatcgggat
    76861 caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcgccca gtttcgtgcc
    76921 catattcaca cgcgcgctgt aaacggaaat tttgtcgata aacgagtccg ggcgggcaaa
    76981 gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacatt gacgggtaaa
    77041 caactgccct tcttcagtga tgtaaatcgc ttcgcccggc gcgacgtcac gcaggaaatc
    77101 aaagcccagc gtatcgagcg ctacgctttc ggaagcgacc atatattctg tacggttctc
    77161 gtcaatatca cgttttccca gtaccagcgg acgaatcccg tttggatcgc ggaaagcaac
    77221 cataccgtgg ccgataatca tcgccacaca ggcatacgcg ccgcggatta agcggtttgt
    77281 ggcagcaatg gcagcgaaaa tattgtcggc ttccagcggg tagtggcgga agttgtccag
    77341 ctcgctggcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga tgtggcggcg
    77401 tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc cgttgtgggc
    77461 aagcgtaatg ccatacgggg agttaacgta aaacggctgc gcttcagagg cgctggagct
    77521 gccagccgta gggtaacgca catgaccaat gcccatattg ccctgcaaac gctgcatatg
    77581 gcgagcttca aatacatcgc tcaccagccc gttcgcttta cgcaaacgga agcagttatt
    77641 ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca ccgttaaggc
    77701 atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac cgcacatacg
    77761 tctttttcct cgttaagcca catctcagag cacttacgct ctgggcaaga aacttgacga
    77821 gctttgcaga taatcaaaaa accatctgat gataaaactg aattgcggga tcagctgtga
    77881 tttgctccag tcttcacttt tcgacacccc ggtaaaggag tcgagaaaga agagaatggc
    77941 ggcaacaatc aacacaccgc gcaacgcacc gaaacagacg cccagcaccc gatcggtgcc
    78001 tgacaacccc gttttctcca ccaactggcc tatcacgaag ttcacgatag caccaacgat
    78061 cagggtagcg ataaacagta ccgcgatggc aatcccattt cgaaccagtt cgtcttcaaa
    78121 gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga aagcacaacc
    78181 ccatgtcacc agcgataacg cttcacgaac aaagccgcgg atcaggctaa ccagagagga
    78241 aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc cacgatttta
    78301 cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa gaaaacgttt gcgtagggat
    78361 ttccttcccg cgcatcaata aaaatggcgc tgaaaaaata ttcaacgcca tcgacttttt
    78421 atgcctttgc ggcatcgtgc aatgcgtgtc ggatgcggcg taaacgcctt atccgaccta
    78481 cggttctaac actgcccaag cctgataaga cgcgccagcg tcgcatcagg caagactgtg
    78541 ttaattcggc gtatagccca ttaccacgcc acttaagcca gaaagttgct tcaactcacc
    78601 cagcgaacct ttcagcttat ctttcgaggc atccggccca accagaatac gggtaatttt
    78661 accctgcact ggcgtggatg gcgacgtata aacccgataa ccggcaccgc gcagcttacc
    78721 gacaatctca ttcactttat cggcattttt cagcgcaccc agttgcacaa cataggcttt
    78781 acccgttggc gctgcttttt cttccacgac cggcttcggt tctggcttcg gcgcaggtgg
    78841 tgcttcaacc tttggtttag gcggctccac cggtttcggc ttcggtgggg cgaccggtgc
    78901 aggttccggt tcaaactcgg tgttattggc tgcaatagtg gccggatcga gcgacggtgc
    78961 tgcggcatca cctgcccgca cctcttccgc tgcgccttcc ggcggctgcg tcggcaacgc
    79021 ctgggtggcg gctggcatca tatcaggctc atcacgatcg cccgctttcg gcaccagcgg
    79081 gatagccgcg aactcatcct gataatgttt tttctgcccg tccagcagcc ctggaagtac
    79141 aatcaccccc agcgccacca gcacgatcgt gcccactaac cgattctgaa acttacttgc
    79201 caccgcttct cctcgcgtca atcacttcca tgacatgtgc gaccgtgtgg aaagaaccac
    79261 acaccagcac ggtgtcttcc gctttagcgt ccgccattgc ggcatcccat gcctgcgcaa
    79321 cgctatcaaa tgatttgccg ttacccaaat gctcaagcag ttgttctgcc gtggcaccgc
    79381 gcggcccttc cagtggcgca caataccagt catcaaccac gcttttcaac caggccagag
    79441 ttccggcaat atctttatca tgtagcatac cgataaccgc cagcacgcgc ccgtttttcg
    79501 gtagcgcttt catacgcccg gtgagatatt ccgccgcatg tggattatgc gcgacatcaa
    79561 aaataacgcg tggcgactcg ctcacaatct ggaaacgtcc cggcaaaatt gcgctggcaa
    79621 tcccgtcgcg aatggcattt tcactgactt ccagcccgct ggcacgcagt gccgccagcg
    79681 ccgttgcggc attcggttgc gggacaagcg gcaacggcag attttccagc gtgccgtgag
    79741 catcgctaaa cgcccagtca tgatcggtga cggaatagtt ccactcaacg ccccgacgtt
    79801 gtaacagtgc acctttttcc tgcgccacat cagcaatggt agaaggcatt tccggctcac
    79861 cgacaattgc cggtttttcg ctgcggaaga tgcctgcttt ctcgcgacca atactttcgc
    79921 gatctggacc cagccagtcg gtatgatcca gcgcaatact ggttactacc gcggcatcgg
    79981 catcgacaat attggttgcg tccagacgac cgcccagccc tacttccaga atcaccacgt
    80041 caagttgtgc ctgcttgaac agccacaacg ccgacagcgt accgtactcg aaataggtca
    80101 gggaaatatc accgcgtgcc gattcaatct ccgcaaaaga ggcggtgtgg gccgattccg
    80161 gcaattcccg gccctgcaca cgtacgcgct cggtataacg caccagatga ggcgaactgt
    80221 agacgcccac tttgtaccct gccgccatca gaatcgactc cagcgtacgg caggtggtgc
    80281 ctttgccatt cgtacccgca acggtaaaca caaatggcgc tggtttcagg acgccaagac
    80341 gcgccgcgac ctggctcacg cgctcaaggc cgagctcgat agttttactg tgcaggtttt
    80401 ccagataaga aagccacgaa gccagaggcg acgcggcttg aggagtgcgt ttgataatca
    80461 tggtatccgc tgattcgtta cggtgagaat agcaaaaggg cagagccagt ggccctgccc
    80521 ttatcagtta tcaggcctca ggttcctgat ccggtaccgg gggtaccact acgccttcac
    80581 gcggcgcttc aggattcggc gctggcagat tcatcaactt cgccagaatg ctcgccagtt
    80641 tcaggcgcat ttccggacga cggacgatca tgtcgatcgc gcctttctcg atcaggaatt
    80701 cactgcgctg gaatctaggc ggcagttttt cgcgaacggt ctgttcgata acacgcggac
    80761 cggcaaagcc gattaacgct ttaggttcag cgatgttgag atcgcccagc atggcgaaac
    80821 ttgcagaaac accgcccatc gtcgggtcgg tcagcacgga gatgtacggc aagccgcgct
    80881 cctgcatttt tgccagtgcc gcagaggttt tcgccatctg catcagcgac atcagtgctt
    80941 cctgcatacg tgcgccacca gaggcggaga agcagatcag cgggcagtta tcttcctgcg
    81001 cctgctcaac ggcacgcacg aaacgtgcac ccacaacaga ccccattgaa ccgcccataa
    81061 aggcgaactc gaatgccgca gcgacaaccg gcattccata cagagtgcct ttcatcacca
    81121 ccagcgcatc tttttcgccg gtttctttct gcgcagatgc cagacggtct ttatacttct
    81181 tggagtcacg aaacttcagc tcatctttcg gctcaagctc gctacccagc tccacaaggc
    81241 ttccttcatc taacaggcta tgcaggcgat tacgcgctgt catacgcatg tgatggtcac
    81301 acttcggaca gacctcaaga ttacgttcca gctcagcgcg gtataaaacc tgaccgcagc
    81361 tatcacactt agtccacacc ccttcaggaa tgctcgcctt gcgggtggga gtaatgttgc
    81421 ttttaattcg ttcaatccag ctcattaggg acctttctgt ctgaacttgg ttcgatgcca
    81481 gttttatctt tggggacgca taatgccatt tttgccccca acagaccatg aatgttgcac
    81541 attaaaacat aacagcccga aactttggat aaaaaagtgg tcgaaccgcg gagttacttt
    81601 ttattttgcg gcgcgtgccg cagcgcgttt gtgacggatt atttcgatga cgcccggcaa
    81661 aatagaaacc acaataatcc cgacgatcag cagcttaagg ttatcctgaa ccatcgggat
    81721 tgtaccgaag aaatagcctg cgtaggtaaa aagcagtacc cacaacagtg cgccgatcac
    81781 gttataagca gcgaaatgac ggtacgacat gtggcccatt cccgcaacaa acggggcgaa
    81841 cgttctgacg atcggcacaa aacgggcgag aataatcgtt ttgccgccat gtttctcata
    81901 aaactgatgg gttttgtcga gataactgcg acggaaaatt ttcgaattgg gattactgaa
    81961 taacttttca ccgaacaacc gcccgatagt gtaattgacc gcgtcaccca caatcgcggc
    82021 gatcagcatc agcactacca tcatatggac gttgagatcg ttggtttcca gtgatgccaa
    82081 cgctccggca acaaacagca atgaatcacc cggtaaaaac ggcattacca ccagaccggt
    82141 ttcacagaac aaaattaaga acaaaatggc ataaacccag acgccgtact ccgcgactag
    82201 ctctgccaga tgcacgtcaa tgtgcaggat aaaatcgatg aggaaataaa tcaggtccat
    82261 atttgcctat gccttgtact cgtcatcttt caggctgtaa ctacgttggc tacagcctga
    82321 aatgttccga gcattattct cgttagtccg ccagaaatag cgggcccatt ggcggttttg
    82381 gaagatcata ccggtcaggg taatccaccg cgaccagata cagcccttcc gcttttgccg
    82441 ttgctgccgc cagcgttctg tcctttgccg ccagcaactc tgctatccag ctctccggct
    82501 ggttgtgggc accgacttcc atcaggctgc cgacaatatt cctgaccata tgatgtacaa
    82561 aggcattcgc tttgatatca accaccacat aaggaccgtg acgcgtgaca ttaatatgca
    82621 taacgttgcg ccacggggtt cgggactggc actgcaccgc acggaacgag gtgaaatcat
    82681 tctcgcccag caagcattgc gcagcccgat gcatccgttc agcgtccagc ggttcgtaaa
    82741 aatgggttac ccctttactc agtaccgccg ggcgcagccg atgattgtag atgatgtagc
    82801 gataacggcg agccgtggcg ctaaatcggg catgaaaatc atcaggtaca gctttaaccc
    82861 aacgcacggc gatgtcacca ggtaaattcg catttacgcc caaggtccac gccgcgtctt
    82921 tgcgctgcgc ggttgtttcg aaatgcacca cttgcccggt accgtgtacc cctgcgtcag
    82981 tacgcccggc gcagaagacg gtgatgggtt cgttcgccac ctgggagagc gccttttcca
    83041 gcttctcctg cacactgcgg acttcattct gccgttgcca gccgtaatac ttactgccgt
    83101 cgtactcaat gcccagcgca attttataaa ctggcagttg ttgctggtcg gacattagta
    83161 cagatactcc tgcaccagtt tctcggcgat ttttactgcc atcagcgcgc cgccaaagcg
    83221 aacgttatcg gccaccgacc agagctggac ttgctccggc ataccgtagt cattacgcac
    83281 gcagccaaca gaaagatgcg gcgtacccga agcatcccct acctgagtcg ggaattcgtt
    83341 ctcttcagag agcacaatat cttcgccctg agcaaacgca tcacgcgctt cttctgccgc
    83401 cagcggacgc agtgcttcaa agttgaccat ctgggcatga ccgtagaata ccggtgcctg
    83461 gacgacgctt gccgaaatca tcagcccttc gtcctgcagg attttgcgta cttcgtcaac
    83521 gatacgacgt tcttcacgca cgctaccttc gctatccggc aacaacggca gcatattgaa
    83581 cgccagttga cgaccgaaga aatcttcttc gtcaatcgga atgccgttga gcaatttcgc
    83641 actctgcccc gctaacgcat cgaccgcttt tttgccctgg gcggaggctg aaatcaggct
    83701 ggtaacactg atacgcgaca aaccgccctg atcgattaac ggtttcagtg ccgccagcag
    83761 ctggctggtc agactgtctg gtacggcgat gacattccgg ttgcggtaat cggtcagtac
    83821 aaacgggttt acttccggca ccaccagcgg tacgtcgggt tcgagagcaa acagtccgct
    83881 gctgtcgatc accaggcaac ctgagttggt cgcttcttca acccaggcag cggtagcttc
    83941 tttgcctgcg acaaaaaatg ccagctgcgc ctgcgtccag tcgaattcag cggcatcctg
    84001 cacggtgatt gttttaccac caaagcgcag ttgttcgcct gcgctttcgt tacgtgccag
    84061 tgcataaatt tccccaaccg ggaactgacg ttcagccagc gtttcaagca gggcttcgcc
    84121 catagcgcca gttgcgccca ggacggcaat gttccagcct tcagacatgg tggtttactc
    84181 cagaaatagc aaagctccct gccaatgtta cagcagagag catgaagaag agattaacgt
    84241 gccggatgat gaacggcgtt aaaacccagt ttacacagca atgatgccgc actggcgtca
    84301 tcacaaatta catacagaga tgaccattca cggcgctcaa gatagttttt gcgcagttta
    84361 tcgaactcac ccggtatccc ggcgacttta cgcagcggtg cgtcatcgcg gcgcacatca
    84421 tacaccaaat gcaccagcct tttcagcgtc ggttgatcga gcggaccatg cagcgtaatg
    84481 cggccaaact caggcgcggg cagcaacgta tccagcgcaa cgtgttgttc atgcccaata
    84541 aacttgctat aggcttcaaa cacttgcgtc gtgccacgcg ctttaccctc caaggtatag
    84601 cctgcgatat gcggcgtgcc gatatccact tttgtcagca attccacatt aagttccggc
    84661 tcgccttccc agacatccag caccacgctt aacttctggc cttcattcag gcaggtcagc
    84721 agcgcggtat tatcgacgac tgcgccacgg caggcgttaa tcagaatcgc tccgggcttc
    84781 aggctacgga tcagtttttc atccgccaga tgtagcgttt tgtacggacc atctttaaag
    84841 agtggcgtat ggaaagtcag aatatccgcg cgctggacta actcatccag cgagcggaaa
    84901 tcaccctcat ccccacggtc ggcgcgaggc ggatcgcaaa gtaaggtttt aatccctaac
    84961 gcttccagtc gcgcctgcaa tcgacgtcca acgttaccaa cgcccacgat ccccaccgta
    85021 cggtcgtgca gtgaaaatcc atcgcgttcg gcaagcatca gcagggagga gaaaacatat
    85081 tccaccaccg caatcgcatt acagccaggt gcagcggaaa aaccaattcc cgcctgcttc
    85141 aaccatgctt catcgacatg gtcggtcccc gcagtggcag ttccaacaaa tttaatgggt
    85201 tttcctgcca gcaaagattc attcactttc gtgaccgaac gcaccatcag cgcatccgtg
    85261 tctgccagtt gagcgacggg gattggacgc ccgggaaccg cgatcacctc acccaaacgg
    85321 ctaaataagt cgcgggcata aggcatattt tcatcaacaa ggattttcac gtttgtgtta
    85381 cctgtatgag acgagagtta accggacaag tgtgccataa tctcgcggcc aggcatactt
    85441 gcgaagattt caggtataag gatacgtaat gatacaacct atttccggcc ctcctcttgg
    85501 gcagccacca ggtcagggag ataacctacc gtctggcgcg ggcaatcagc ctttatccag
    85561 tcagcaacgt acttcgctgg aaagcttaat gacgaaagtg acctcactga cgcaacaaca
    85621 aagagcagaa ctgtgggcgg gtatcaggca cgatattggt ctgtcgggag attcaccgct
    85681 gctttcgcgt cacttccctg ccgctgagca taatctggcg caacgtctgc tggccgcgca
    85741 aaaaagccat tctgcccgcc agcttttagc gcaattaggg gagtatttac gtctggggaa
    85801 taatcgtcag gcggtcacgg attatatccg tcataacttt ggtcagacgc cgctgaatca
    85861 gctctcaccg gagcaattaa aaaccattct caccctgttg caggaaggga agatggttat
    85921 tccgcaacca cagcagcgcg aggcgaccga ccgtccttta ttaccggcgg agcacaatgc
    85981 gctaaaacag ctggtgacca aacttgcggc ggcaacgggg gaacccagca aacagatctg
    86041 gcaatcgatg ctggaacttt ccggggtgaa agatggcgag ttaattccag cgaaactgtt
    86101 taaccatctg gtgacctggc tacaggcgcg tcagacgcta agccagcaaa atacgccgac
    86161 gctggaatca ctacagatgg cgctaaaaca acctttagat gccagtgaac tggcggcgtt
    86221 atcggcatat atccagcaaa aatatggcct ttctgcgcaa tcatcgcttt cttctgccca
    86281 ggccgaggat attcttaatc agctttatca acggcgggtt aaagggattg atccgcgtga
    86341 tatgcaaccg ctgcttaatc cttttccacc gatgatggac acgttgcaaa atatggcaac
    86401 gcgtcccgcg ctgtggatac tgttagtcgc gattatcctg atgctggtct ggctggttcg
    86461 ttaaccccga cgaaatgaca gtatcgtgac aataataccc agcaccgcag agatcgcccc
    86521 ggcaagaaat accgaagagt aaccaaacgt ggtcgccagc attcccgcca gcggcccgga
    86581 gacgccgagg gcgatatcct gaaacgcggc gtaaccgccc agtgcggtgc cgcgaacttg
    86641 tgaggggacg cgtttaacca cctccacgcc cagcgcagga aagataagcg aacatccggc
    86701 tccggttaac gccgcgcccg ctaatgcgac ccatgcacct ggggcttgcc agagcagcaa
    86761 caagcccacc gtttctacaa gcagagagac aatcgccact ttcacgccgc caaaacggtc
    86821 cggcatccag ccaaacatga cgcgcatcac gacaaatgcg ccgccaaacg cggtaagagt
    86881 aaagcccgcc atcgcccatc ctttgctggc aaagcagagc gaaacgaaag tcccgataac
    86941 cgcaaaacca acgccttgta gtgccagccc taaccctggt ttccagataa gcccgacaac
    87001 gctccacagc gatggacgtt ctcccgccag ggccggtact ttgcgcactg tgccgttaca
    87061 ggcccacgcc agtaagggta ataccattgt ggtgatcgcc agtgcggcaa aaccgtaatg
    87121 gctatgaatc aacaggccaa gcggcgcacc aacagcgagg gcaccgtaaa tcgccattcc
    87181 gttccatgac atcactttgc cagagtgttt tggccctacg atgcctaacc cccaggtcag
    87241 agcgcctgtc agtaactggc tttcaccaaa cccaagaatc aaacgcccga tgaccaacag
    87301 ggcaaatttg aacggtgcgg agacaggcaa aatcgccgcc agcagcaacg cgccgccagc
    87361 cagaccacaa gctaacatcc cctgaagcgc cgaacgtttt gcgccatatt gatcggccag
    87421 tcgcccggcg taaccacgcg tcagcaccgt agccagaaac tgaatcccga cggcaatgcc
    87481 gaccatggta ttgccatagc ccagatcatg atgaacaaac agcgggataa ccggcaacgg
    87541 caaccctacg gtcatgtagg tgagaaaaac cgcaaaagcg atgcggaaga gcgaaaaact
    87601 ggcagaagat cgtgtttcgg tttggcttac agcagtcatg cattactcca gaatgcagcg
    87661 caaggcgagg agtatccccg tctcatctct ctggtttcag ggttacggtg cgttggcagg
    87721 atttaacgcg tacgtctttt cagaaggaaa tcgacaaagc gggaagtttg cctagaactg
    87781 gtggcgattg tcaatgatgt gaaaaaggga accatcaggt tcccttttgc gttagtgccg
    87841 gaggccgcat tggcgcgtaa cgtcggatgc gacgctggcg cgtcttatcc gacctactcc
    87901 ggcctactgc gaattaatct ttcagcttgc gcattaccag cgtggcgttg gtgccgccga
    87961 agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg gtcacgatgt
    88021 tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg ataaagccgt
    88081 gttccagcat cagcagagag tagatagctt cctgcacacc cgcagcgccc agtgagtgac
    88141 cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact tcacggatag
    88201 ctgccagctc tttcacgtcg ccaaccggag tcgaagtacc gtgagagttc aggtaatcga
    88261 ttggagtatc aacgccatgc atcgccatct gcatacaacg tactgcgcct tcgccagacg
    88321 gagcaaccat gtctgcacca tcagaggttg cgccgtagcc aacgatttca gcatagatgt
    88381 aagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg ccgccgccag
    88441 cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc ggggtgtcgt
    88501 tgtatttagt agacagcgca cccatcgcgt cgaattcgca ggccatttcc cagcacagct
    88561 cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc tctactgcgt
    88621 taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta acgccatgaa
    88681 ttttaaacgg cgtggcgaga caggcagaaa cgccggatgc catcgcttta gtgaccacat
    88741 acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg aacacctgga
    88801 aacgcgggga accgccgccg gaacctgcaa tcaggccaac gcgcgggtta ttctggtaag
    88861 cttccggaga gaggcccgca tcagcgattg cctgctccat agaaaggaat gcataaatgg
    88921 atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta tccagtttta
    88981 tgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag aaagtgatcc
    89041 ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg atgctggaaa
    89101 caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag tcgcacatag
    89161 agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg atcagccatt
    89221 taatagagaa atttgcgcag ccttacacac atcgctaaga tcgagccacc gcctgtaaga
    89281 cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt taatgctgag
    89341 ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa cgggctggaa
    89401 gagacgcgtt atgtttttct gggaggtaac caattagagg tacgctttcc tgagcatcca
    89461 catcctctgt ttgtggtagc agagagcggc ttcggcaccg gattaaactt cctgacgcta
    89521 tggcaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca acgcttacat
    89581 ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc gcatcaacac
    89641 tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat gcccttgccc
    89701 ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc tggatttatg gtttggcgat
    89761 attaacgaac tgaccagcca actggacgat tcgctaaatc aaaaagtaga tgcctggttt
    89821 ctggacggct ttgcgccagc taaaaacccg gatatgtgga cgcaaaatct gtttaacgcc
    89881 atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat ttacgtctgc cggttttgtc
    89941 cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac gtaagggctt tgggcgcaaa
    90001 cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc cacgccgtgg
    90061 tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg tattgccagc
    90121 gcgttgttgt cgctggcgtt attacggcgc ggctggcagg tagcgcttta ttgcgcggat
    90181 gaagcccccg cgctgggtgc ttccggcaat cgccaggggg cgctgtatcc gttattaagc
    90241 aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc tcgtcggttt
    90301 tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca gttaggctgg
    90361 gatgagaaaa gccagcataa aatcgcacag atgttgtcaa tggatttacc cgcagaactg
    90421 gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac aaattgcagc
    90481 ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg taatgtgctg
    90541 gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca gaatttatcc
    90601 cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac acacagcgta
    90661 gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacgtcgac tctcccggtg
    90721 tattcggttg ccgggcaggt cagccatatt ccgacaacgc cggaactggc agagctgaag
    90781 caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca acatcattgt
    90841 attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga cgatcagcag
    90901 cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa agaggttgat
    90961 gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca tctgccaatg
    91021 gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt ggcggagcaa
    91081 aaagatgagg cggtaagcgc gccggttttt gacgatctct ttatgtttgc ggctttaggt
    91141 tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc tggcggcgca gatgagcgac
    91201 gaaccgattc cgatggatgc cagtacgctg gcggcgttaa acccgaatcg gttatgggtg
    91261 cggaaattgt tgaagggtaa agcggttaag gcgcggtaat ctgctctcgc agtgtttgcc
    91321 ggatgcggcg tgaacgcctt atccaaccta cgtgtgacgt gtaggcctga taagacgcga
    91381 taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tacagctgtg acgcctgctg
    91441 aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca attcaccggc
    91501 accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag cggagtggtc
    91561 atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat acccactggc
    91621 aaacaactca tcatcactgg catggtcaac catgccgtcg attaatgcca gaatgcgtga
    91681 ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcgctggc ttcagttgag
    91741 cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat aattttgtag
    91801 tgctttaatg aagcgtgccg ggcgttccgg aatgccattt gtcagataat ccatcacctg
    91861 cgcatgtacc cgccgttgaa acaccacacg atccggttcg aaatcccctt ccagattgtc
    91921 gcaactgacg ttaaacggat atccagccgc cacgcagaat aaccagtcga acgcctgcgg
    91981 cttcacttca acatcttcaa actggctttg cgtctgggca tcgcgtccgt ccgggcaata
    92041 ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc agtgcgaaat
    92101 ctcatgaatt gcgctggcat aaaagccatg agcaaagacg attcggttat acggtacttc
    92161 cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg tattaaattc
    92221 atcggcaaag cagctattaa aaatttcaat caactgctcg tagtggtgtg tactgttcat
    92281 tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag taatttggca
    92341 ctcatcaccg ccgagacgat aacaatcatc gggcggatca gcttttgtcc tttgctcaac
    92401 accaaccgtg aacccatgcg cgcgccgagg aactgcccca ccagcatgac aaagcccgtc
    92461 gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt tgacgttgcg
    92521 ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag cataacgaag
    92581 gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa accgacgcag
    92641 ccaccggcaa ttaacgcaaa gggtaaaccg tacatccggc gctggcgatc ttcttcaccc
    92701 agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg caaaatctgc
    92761 cgtaagacat cagcctgaac gtactgtacc aacagtgcgc cgctcattga gccgacaaag
    92821 gtcatggcaa tattgagttt ctgatcgctt aaactaacca ctttgcggcg aataaagtag
    92881 atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag cgcgttagcg
    92941 ggagacattc ccgcagccat caatgccgga atggtgagta acccaccgcc accggcaatc
    93001 gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa cagcggagaa
    93061 accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt agcgcctggc
    93121 aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc ggttttggag
    93181 gttcaaacca gctttgcagt tctgccccgc aaccatcgcc tgatggcggt aaaggttgat
    93241 cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc gcgcgatgct
    93301 ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca aggcaaagtt
    93361 gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg gcggcgagtt
    93421 tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta ccgtcgcggg
    93481 aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga gttttcggca
    93541 gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg ccgttgaaac
    93601 gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc agattgctca
    93661 cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa tagcgacgct
    93721 gatcggtacg catgacctga taatgttcgg actgtatcgg caacgtgtca gcgccgacaa
    93781 tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt tgggttattt
    93841 tttgccacgg cgtcgctgcc aggctagcgc tactggcaag cagagccagc agcgcaatcg
    93901 cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca ttttgcgccc
    93961 gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg atcggcactg
    94021 cacggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact tcttcgccaa
    94081 agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc gccatatgag
    94141 caatgatttg ctgcccgctg ctgataccgc cgaaaatgcc gcccgcatgg ttgctctgga
    94201 aaccctcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact acgtcaaaac
    94261 cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg gcgatgtcgg
    94321 catccaggcg gtcaaagacc ggctcgccaa gtccggcggg gacaccactg gcaacaacgg
    94381 tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt tcatctaacg
    94441 cgtcgatttt gtcggggtcc gggcagaaga acggattttg ttcaaccagc gaccagtctt
    94501 tgatttccag cggaatgtcg cccatctggg tcaggcagcc gcggatttca ataccaaatt
    94561 tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg gtttcgcggg
    94621 cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg taggtgtaat
    94681 cagcatggcc tggacggaag acgtctttaa ttgcgctgta atcctgagaa cgctggtcgg
    94741 tattttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa acaccggaga
    94801 gtattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc ccagggcgac
    94861 gacgatcaag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc ggaacaccat
    94921 cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa ggtggttacg cgaaagagtt
    94981 gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt gtttacttaa
    95041 tctttataaa tcgcgaaatg ttctcgtgcg gcaagaagct gctctttagt gagcataaac
    95101 acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatcggg atattgttcc
    95161 ataagatgta ccatgctgtt gcccacttca caaatcaaca cgccatcatc agcaaggtaa
    95221 tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc cgtcggtgcc agatgccagg
    95281 cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc cgcgtcgaca
    95341 tacggcgggt tagtgacaat cagatcgtat tgcactttcg gcaaatcacg gaacagatcg
    95401 gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg ttcggcaacc
    95461 gccagcgcgt cgggagagat atctaccgcg tcgacttccg cttccgggaa ggcataagca
    95521 caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg cggttgcttg
    95581 ctgatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg cggcaccagc
    95641 acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt caggtaagcc
    95701 accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacggtg tttttcgctg
    95761 gaggtcagac gcgcggtgcg catatcttcc gggatatcca gcggcaggta gagcgatggt
    95821 aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta ccagatattt
    95881 gccgcgctga agcggcttac cgaccagcgc aacatgtcct gaatggtttg cagctcattt
    95941 actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc gcaataattt
    96001 cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg tgagtgagga
    96061 aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg aaaattagaa
    96121 atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag gctctgtttc
    96181 gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac cgaccgcagc
    96241 gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct gatgccagcc
    96301 attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg aaatatgttc
    96361 gcccggatgt cagccatttt gaggcgaaga aactgcgccg cggcgattat tcaccggaat
    96421 tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg ggcgcgttga
    96481 ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgcatggg cacgggaagc
    96541 atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg atggcatttc
    96601 accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt gaagtggaag
    96661 agtggttgcc gccggagttg ccctgaatgc tggcagcaaa tgccggagca gcgtaaacgc
    96721 cttatccggc ctacaaatat gtgataattc aataaattgc aattcacttc gtaggcctga
    96781 taagacgcat cagagcaata ctacgaactt cagttcattc aatacatact gaaaacgaca
    96841 cgtcgaatca atcggttgcc cactactggt attcacattc accacaatat cgtgtagtac
    96901 tttatccgtt accggatgac gagttggcgt aaccgttacc gttgatttat cacaaccgta
    96961 agaggtaaag gttaacaatt ctgataccgc gcggcagtat gctcctctgc catcattatc
    97021 agaaaaatta ctgccatcac agagtctgcc gccttccatc aggtaggtcg aaagcgtggt
    97081 ggtcactgtt gaccccgtgg actgaattcg taacgcctta ttatggctga cataattttc
    97141 actccaggag atatcgtatg aagcgaccgg tatcgttggg catattgtgt tatgcactgt
    97201 aggggccgta tagcttccaa atccgggtaa atctgtatag gaataaaagg atgcgatcca
    97261 agggcaacca taatcatcag atgcaacggt aattttaaac gtcatatatg cacaacgaaa
    97321 aattttcgta tctatgccag ggcaatttga gttattaatt gtatcaaagt cagtcagtac
    97381 caaagttgga gctgaacccg acccagacca ggaaataacc cgcaacgtat cgcctttata
    97441 cgcaaaagaa tcatatttcc catatgcaac ctcctttggc gttatcaccg taacattgaa
    97501 aacactgctc gctacagcac cggttacctt gtccatggca taataaactc tcacactgtc
    97561 aaaactcgca ctcataagtt tatctccagc ataactctta gatgcgccga caacactcag
    97621 aacgagcagc attaggctcg cgaaaattat ggtccatttt ttcatttcag aaatactcaa
    97681 aggttgccgt cactgtggca ctaaagtctc cggacgttac atctcgcccg ctctttgcct
    97741 gtaactaggt attaaaattt aggctgttat ttccctgatt cagcgggagc gtgacgcctg
    97801 ttgtattgtt gattggcacc gatgtgcctg ccgcagtttc aataccaatc ccagcgccgg
    97861 atgctgatga cgaggagtca aaggccagaa agccgggtag agcactatct tctgttcctg
    97921 tcaacgttac tttgacatta aacaatgtag aactatgaca atctttcaat tgaaacacca
    97981 atggcactcg ctctgactgc ccaaggttca ttaaatcgct ggcggcaatg gcaggaaaat
    98041 gtacttctgc caactggcta ccattaatca ctggcgtaca cgatttactg attaaattgc
    98101 cggtaaagtg caaattatct cctgctattg tggttccact gcccagcaac atcaggctac
    98161 taataaaaaa caacgtacgc tttttcttca ttggtaatct acctgtaacg tggcccaggc
    98221 ctcaaagtcc gcctctggca gaatgacacc actctttttt actggcacag ccgttaacac
    98281 cggtaaatcg gtttcattca ccacaagtgg cgtattgatc gtaaaactct gccctgcctg
    98341 tttgagctga atgcctaaac cagtgatatt gctgcttact gcagcattat caaaatcgct
    98401 tggtgtgcca ctccagctca aggtcatttc cattaatggg tcgcgaaccg ccgtatcgca
    98461 ggtaatggaa tacggcacgg tttgctcata attgctgcca ttaatgtcat caataagcac
    98521 atcctgaaaa gttatttcaa tggtttgagc gttgctaatc gtgcagttag gcggctgcac
    98581 taaataaccg tgaaaagaga cgtttgagag tgccatcgag caaaagcccc acagcaaaat
    98641 aagcgagatc cgtttcattg gtaatccacc acaattgtgg cactggcgtt aaattcagct
    98701 gccgctaacg ttgtaccact ctgtttcacc ggcacagctt ccagaacggg aactcccgag
    98761 gaaaaattaa atggcaacca tgagccagag gttaagtcga gtattgtgtg atcactactt
    98821 ttctgaaccc gaataccaaa cccaggaata cccgtgctga taactgtttc accgttaatc
    98881 acgaccgttg tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt gttacaaaca
    98941 agcgagtagt caatgggacg tttataatca accctattga tcttattaat gaatacatta
    99001 ccaaactcaa ctgccgcacc atttaccgta caaggaggag gggcatcgac aagcacccgt
    99061 aaattcatac ttactgactt actcgctgca actgcaaagc cgctaccaac acaacatatc
    99121 agtaacccag ttaccgccca gacagaacaa gatcctttca tactggctct cccggcctca
    99181 gcctttgcgg ctctgctctt catcgaccta gcaactgtta ccgttgcaag tgaaaaagag
    99241 cggcatccga gcgccatagt cgttgacata ggtcagtacc ggcacgctac ccatcttcac
    99301 attcagcggt gcagaggttt tcggcgacat cactattggc gagaaaccgg ctgccggatt
    99361 gccatttttc tgtgtgctgg cattactgaa aatgacgtaa tacgacgtcg ggttattgac
    99421 ggtaaattcc tgaccgttgc gggttaaagt aaccttatat tgataggggt ttttcatgct
    99481 gacgttctcc agcgccttcg gtcgccagaa caacttaata cgcgtttgta gtgctatttg
    99541 cagcgtgttg gctttgtttg attttggcgg gatctcacgc acgttgaagt agaacaggct
    99601 ttcacgatcg gcaggcaatt tgttgatatc cggcattccc tgcactttta cctgcccgtt
    99661 catcattgaa tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac tgatcttgtt
    99721 acccttttca tcttcgatcc agctttgggc cagataaggc agtttcggat cgttattgcg
    99781 caacgtcacg ctgattgatt tgtcgctttc attaaatatc aaacgggtac gatctggcgt
    99841 tacagaagcc tgggcagaca aactggcagc gctaagcagt aacgctagcg tcgtagcacc
    99901 caatttggca gagcaaagta aatcagacat ggatcattcc ttaatcacgt tattggttag
    99961 ctgaaacaga aacaggggga ttggtgggtg tcacctgttg cgtctgctcc tgaatgacag
   100021 gctttatgtc atcaggaaca accggcgcga cgttgccttt atgctggcat ggcagtaaca
   100081 agccgttaaa cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa tgcgcaacac
   100141 cactccagaa cacactcatg tgttcgccag gtttcacccc agccagataa acattgccgt
   100201 catcatcgac aaggcccact gtctgctcgt tatcattttt tacttctgcg ccaaacggag
   100261 gatggctgcc gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg atgacggcaa
   100321 atttgcggta gccaatcgcc ccttcagtta gcgtggcttg taccaccgac tgggttgctt
   100381 cggcgttttc cggcagtttg ttgaggtcga tatacgcctg attgcggtaa tagttattga
   100441 cgtcagaaac gacggcttta ccaaacatat tggtataaac agcagcaccg ttaccttcta
   100501 ccggaacatc ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc atattctggg
   100561 tacggtgaag tgcgccaccg tgggcagtaa acgttgcgcc gccctgtaac gacaagcccg
   100621 cagaggtgta ttgcccttca tggtagttcg cactgaggtc aacctgcgcc agcgaaccat
   100681 catggctgta atagccgtca acgctggtgt gtttgtcact ggtgccgacg ttcaactgat
   100741 agtgagtcgc gtcatcgaca cggctgaaat aaccgacctg actgctgtcc gtcccactgc
   100801 catagtaccg ttatagctaa cggtactgtt gtcgccccac ggcatactga gcgaaatgta
   100861 catgcctttg tcggcctggt tgtcatactc gtaacggtag ccagtcatcg agatgctgac
   100921 attacgaata ctgcccatat tgaaatagtg cgagagcatg atgttgtagt ttgtctgctc
   100981 ctcgcgatcc cagtaggtat ggcgggtata gttgagataa accgaaacac ccgcatcgcg
   101041 gaagttctgg ttataagtgg cggtgtacat ctctttgtcg ttgcccgtgc gtaccattcc
   101101 gctgtcactg gcatccagat actcgctcat ggtcataaag ttctcttccg agaagcgata
   101161 tccagcaaaa gtaacgcggc tgttgagctg gtcaaagtct ttggaataac tcacacggaa
   101221 ggagttaccg tccagcgaac ctttgccata agcggtgtct ttatccagtt tggtgtgcga
   101281 gtgagtaaca tcaaacgcga ccgcgccgaa tgtagacaaa tcgcgaccga caccaagcgc
   101341 cgcagactga tagttttcat ctcccagtgc gccgccatat aacgaccagc cgttagcgat
   101401 cccccaggaa gcttcagcac cagaaaaaaa ttcaccctcg acatggtgtc cccactcttg
   101461 cggacggccc atcatgatct tatagcgaac ctgacctgga cgagtgaggt atggcatcga
   101521 ggcggtgctg atgtcatatt cctgcacctg gccgttctgt tcttcaatgc gaatatgcaa
   101581 cgtaccggag acagaatcac caagatcctg aatacgaaac ggtccagccg gaacctgcgt
   101641 ttcgtaaatc acacgcccca tctggctgac ggtcactttt gctgtggtgt gtgccacgcc
   101701 ggaaatgtct ggcgcgtagc cgcgcagatt gggaggcaac atttgatcgt cagtactgac
   101761 actgccacca acatagttaa aaccatcaaa aatatcggat ctgaggtaat cctcgcccag
   101821 cgccagtttg gcttttaatg atggtaacgc ccgcctggca tagtagcgac tccactccca
   101881 ttttttttga gtttcatcgc cgctgaattc atcgtcatca ttactgcgag tatgttgata
   101941 gttagtctgc cagtcagcac gcatacgcca cggccctagg ttaaccccaa ccgtcccgtt
   102001 gccgctgatc tcgttactat catcaccgcc attttcttcg tgccgggttt gtgcgttgat
   102061 gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg gatcccagtc
   102121 gggataagta tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg actggcttaa
   102181 atcagcctta atttccatgc cttccagttg accggatttc aggcatttag catcgtggct
   102241 ccattgcaga tttttcgcca cgtcttcttt taaaccaaac tgcgctacca gttccggtgt
   102301 cagacaagca tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa tatcgtactc
   102361 ttccgccaat ggctgtttat ttagttgaac ctgtaaattg tattttccgg gctcaacata
   102421 tccctggctg gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt ccagaaaacg
   102481 ggaatcaaac tgaatgtcgt cttcagccca gacactgcta taactccctg acattgccag
   102541 cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca tacacgtttc
   102601 tatccgtttt tattcttgtt cggtcaaaat ccatttaaat cccccatagc agtgtcgcta
   102661 tgggggtatg ccttacaggt aagtaatctg gaaggtggta tttgcttcaa aattacccag
   102721 atctggcgca tcagctgcgc cgaccagcca ggctttaaag ttcagtgtct gcttcgcttt
   102781 gcctttgttg gtagaagtgt ctttcactat tttctggtca atacccatgc cgcttttgta
   102841 agaggtaccc agagagtcac cgatcgccag gctaacattg ttaaatgccg caccggtatc
   102901 ggtattgaaa atggtgtaat aattgccgct atttgcagaa gaaacggtac cagtaaaggt
   102961 agtggtcatt gtttcctgag tatcaaatac gcaatcttgc agacgaatct ggaaactttt
   103021 cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg atatcaccca tatcaacttc
   103081 cagtttgtga tcgtccggaa cgatagaaca cactgagtct tcaatggtgc cgtaaaaacg
   103141 tgcagtaccg ttgttacccg cagcgattgt tgccgtagag gtgaacacgc ccatcaccat
   103201 tgccgcagca atagctgttt taacaaactt actcattatt tgtccttaaa ctataaataa
   103261 ttaaaaatat aaacattgca atacattgat tcagtcaata gccaatgttt atacccagcc
   103321 attgaatacg gcatgcgacc caaaaagaac aggtcgtaga aaattgtatt cattggtgtg
   103381 gggccgcata gtgatgtttc cacacccctg atccaattta attttactaa agagtaaatg
   103441 tagatttaat taatatattg acagaagaaa ggttatctta aggacatcct taaaaaatac
   103501 ggaataagta tttagaaaaa atagaaagta tatcaataat gaatatcaac agcttaaaaa
   103561 acaaacaatg aacatataat gcgattggca tttatccact tttgacaata taatatataa
   103621 acactatagc cagaatttaa tcaagtttca attacatcct gcctgattat tcaactctat
   103681 tttctctatt atctgtatta ataaatgaca ctgagtgtca aacagacatt attcatttca
   103741 acatgtaact ttgggcaaaa tcaagatacg cgcagaagaa taatttccga atgaatgtag
   103801 aaagtgtttt atttatagat aaggagagtc ttcgatagta gtgaggttga cagatatccg
   103861 ctgcctaatg agcaattatg cccaatgaat ctacctcatt gggcataatt tggaacacat
   103921 cagatagctt ttgccatctt cagattacac gggctcatct gccagttgaa tgtaccgttt
   103981 ccgctttcat caagcgttac gctggcaatg gccgaggtgg taaacatagg cggcgtttcg
   104041 cccggacata actcggcaac cagatacccg actaacggta agtgagagat caccagtacc
   104101 gaagcaacac cttcattggt caacgcctgc aaataggcac tgaccagacc aacatcgccg
   104161 cagggcgtta actccggcag aacttctgca ctggaaggca ggttcagaca atcccctact
   104221 tcttccagtg tttgctcggc tcgcaggaac gggctcacca gaacgcgttc gatttccact
   104281 ttttgacctt tcagccagtt cgccatcagg cgagattcgt cacaaccatt agtggtcaga
   104341 ggacgaaccg aatcactggc ggcatcgagg gctgcgtcgc cgtgacgcat gataaaaact
   104401 tgcatattgc accgcttttg ttaaccagat tcgcccgttt tctttcatct gaaaccagag
   104461 aaagaaaacg gtggccggca ttgtgcctta tccattcacc gaatgaaacg ctgtttttta
   104521 cctcaatggc gtaagtatag tcaatatgcg tttacatttt gaccaacact ccgccattca
   104581 gcgcggattc atatagcttt gaccttctta ttgcaggtca gttgcagttg ttttccaaaa
   104641 actttcccca cgcgcgccca tctcgaccaa acgctcgcaa ggggtaaaac gggaaccata
   104701 ctgcgtggcc agtcgttgca ttattgcaac cacttcgccc gcgccgagag aatcgatata
   104761 gcggaacggt ccaccgagaa atggcggaaa accaatgcca aataccgcac caatatcccc
   104821 gtcacgcacg ctacggataa cctgctcatc aacacaacgt actgcttcat tcagcatcaa
   104881 catcacacac cgttcagcaa cctgcggtgc ggagagtcgc ccctgccctt gtgcgccaat
   104941 gagcgggtaa atggcaggat cgacctgttt tttgctttta cgccctttct gaccataaag
   105001 atagaaaccc cgaccatttt ttctgccttt gcgatcgtcg ttcaaaattg aagaaacaac
   105061 atttgcaggc gcgctaaaac gttctccata agcggcttcc agtacaggaa taattttagt
   105121 cccggtgtcg attcctacct catccaaaag ttggattggg cctaccggaa aaccaaattt
   105181 cactagcgcg gcatcaatgt gctctacccg ttcaccttcg gtcagcatgc ggatagcttc
   105241 attaatgtaa ggcgctaaga tgcgattgac gtaaaacccg gctttgtcac gcacgacaat
   105301 gggcgttttg ccctgttttt tcgccagttt taccgtagtg gcgatggttt gcgccgatgt
   105361 acccgcatga ggaataatct ctaccagcgg cattttttcc accggactga agaaatgcag
   105421 gccaataact tgctcaggtc gcgcggcgtg agcggcgata tcaccaatcg gtaaagatga
   105481 cgtgttcgaa gcaaaaatgg tatgagcggc gcaattttgc tccacttccg ccaccatctg
   105541 ttgttttaat tcgagatttt caaacaccgc ttcaataatc agatcgcgat gggcaaagcc
   105601 gcgatagtcc gtcgttccgg agattaatgc cagctgtttg tcacgttcgc tggctttgag
   105661 atgacgacgg cgaactttgc cctccagctg atcccaactg tacttcagcg catgatttat
   105721 gccctgcggg ttgatatctt taattctgac cggaagcccc gctttacaag cagtgacata
   105781 agcaataccg ccgcccatca agccaccacc taaaattccc acgctgttta atggcgcagg
   105841 cggtgcatca ctaccgggat ctttcttcac gtccgtactg gcaaaaaaga tactacgcaa
   105901 agcttgcgat tgcggcgtca tcgccagttc gccaaaggcg cgagcttcag cgtcatagcc
   105961 gctgctggtg ccctgcgcta atcccgtttc aacaacctcc aggatgcgtt ctgtcgccgg
   106021 ataattgcct tgagttttgt gttctgtttt cttgccgacc attttgaaca gcagcgcacg
   106081 acctaacggc cccgccagaa tacgctcgcg tacaggtaga gggcgggaag atgggcgatc
   106141 cttcttcgcc agctcaacag cggcttccag caaaatggag tgcggaacaa cgtcatccac
   106201 cagccccagc tttaatgcct gtttcgcccg aagttgtttt ccggtgagaa tcatctctaa
   106261 tgctgtactg acgcctatca gacgcggtaa acgctgggtg ccgcctgaac cgggtaacaa
   106321 tccaagttgt acttcaggca aaccgagcac cgttttggga tcgtcagtac aaacgcgacc
   106381 gtggcacgcc agcgccagct ccagcccacc gcccaggcaa gcgccatgaa tagccgcgat
   106441 aaccggaatg ggcaaagcat gaatctccgc catcaactgt tggccctgcc gcgccagtgc
   106501 ttccgcttct tgcgccgttt tgcagttgcc aatcatgttg atgtctgctc cggcgataaa
   106561 attgtccggt ttcgcggaga taaacaccac gccgcgcaac tctttgtttt cacggagttg
   106621 cttaataatg gcgcgcacct gcgaggcaaa ttccgccttc agggtattca ttttctcatc
   106681 cggtacgtcg atggtgatga tagcaatgtt gtccagacga acattaagag taaacgctga
   106741 tgccatttcc attattccgc ctccagaacc attgccgcgc caagcccacc ggcagcacag
   106801 gcggtaacta aaccaaatcc accgccgcga cggcgaagtt catgcaatgt ctgggtaatc
   106861 atccgcgcgc cggtcgccgc gaagggatgt ccgtaagcaa tcgaaccgcc aagcacgtta
   106921 aatttgctat cgtccacttc gccagtggca tgtgcacgcc ccagtacttc acgagcaaaa
   106981 cgttcactac ccagcaactg aatattcgcc agtgtctgag ctgcaaaggc ttcgtgcata
   107041 tcgatcaatg tcagttcgct catcgtcaaa ccggcacgtt ccagcgccag cggtgttgac
   107101 caggctggac cgagcaacat gtcctgccag acatcaatcg cagtaaatgc gtagctgcgc
   107161 agatacccca gcggcaccag ccctaattct ttcgcccggg attcagtcat caggatcacc
   107221 gctgccgcgc catcggtcag cggcgtactg tttgccgccg ttaccgttcc gtgtttgcga
   107281 tcaaacgccg ggcgcagctt tgcgtaatcg gcaagcgagg aattaccgcg aatattgttg
   107341 tcttcgacaa gcggttgttt ataaggaggg ataaaggcag tcatcacctc ttctttgagt
   107401 tttccgtctg accatgcctg agcggcacgc tgatgcgaac ggtgcgctaa tgcatcttgc
   107461 tgttctcggg tgatgccgta ggttttcgcc atttgttcgg cggtgtcgcc catccgcaag
   107521 ccagtagaat attctgctac cgcaggcggt acgggcatta agtcgcgcaa acgcaggcga
   107581 gagaagagtt tcaggcgctg gctcatggtt cgagctttgt tgacatcaac cagcacgtgc
   107641 gccagttttt tactgacgcc aattggcaat acggaagagg aatctgcccc accggcaatc
   107701 cccgctcgga tagttcccgc catcaggctt tctgcgacgt ttgcaaccgc ctggaaactg
   107761 gtagcgcaag cgcggctgac gctgtaagca tcggtatgca cattcattcc ggtaccgaga
   107821 acaatttcac gcgcaatgtt gggggcttca ggcatttgta cgacctgacc aaagaccagc
   107881 tgttcaatca cttcagcggg gatctcgctg cgtgccagca gttcgcctac caccatcttc
   107941 cctaaatcaa ccgcgggaat gccatgaaaa gccgtcgcct ggcgggcaaa aggcgtacgt
   108001 aaaccgctaa caatggcgat acgatcgccc tggcgggtaa ccagtggtaa aacctgaccc
   108061 ataacactcc cctgtaaaaa aataaataaa gtggtctgac ctgatcatag tattaaccat
   108121 ttttttacat ttagccaagc ggagaaaaag gaaagtggga gctatgacac agtgaagaca
   108181 gaagaaagaa aagaaaacgc ccctgccagc aactgacagg ggcgtacgta cccgagagga
   108241 attaacgcag acccagctgg aagatcagca tttcagcttc gcaggcaaaa gtgaaatcga
   108301 tatccaggcg cacaccgtca gactcttcag tgaaagtcgg ggagattttg cacggttcgg
   108361 attccacgct acgggatttt tcagtcagcg ccgccagcgt ttgttctgct tctgcgcggt
   108421 ttgcaaacac gcggctgtaa gacgcggtgc agtcggagtt gtccataatg gtgccaacat
   108481 ccatacagca gcaaaccggg gtttcatcag cactacattt actcatcgtt gatttcctct
   108541 gtatgtgcac ccaaggtgcc agataaacgt tgtggatatt ttacgcttcc ggaaagtgct
   108601 gctccagttg ttaattctgc aaaatcggat aagtgaccga aatcacactt aaaaatgatc
   108661 taaaacaaaa ttcacccgaa tccgcgagtg cgcaacctcc aaattttgcc agctggatcg
   108721 cgtttcttag atcatatttg aaaaaagatg gaaacatact tgcaacattc tagctggtcc
   108781 gacctatact ctcgccactg gtctgatttc taagatgtac ctcagaccct acacttcgcg
   108841 ctcctgttac agcacgtaac atagtttgta taaaaataaa tcattgaggt tatggtcatg
   108901 agccagaaaa ccctgtttac aaagtctgct ctcgcagtcg cagtggcact tatctccacc
   108961 caggcctggt cggcaggctt tcagttaaac gaattttctt cctctggcct gggccgggct
   109021 tattcagggg aaggcgcaat tgccgatgat gcaggtaacg ttagccgtaa ccccgcattg
   109081 attaccatgt ttgaccgccc gacattttct gcgggtgcgg tttatattga cccggatgtg
   109141 aatatcagcg gaacgtctcc gtctggtcgt agcctgcaag ccgataacat cgcgcctacg
   109201 gcatgggttc cgaacatgca ctttgttgca ccgattaacg accaatttgg ttggggcgct
   109261 tctattacct ctaactatgg tctggcaaca gagtttaatg atacttatgc aggcggctct
   109321 gtcgggggta caactgacct tgaaaccatg aacctgaact taagcggtgc gtatcgctta
   109381 aataatgcat ggagctttgg tcttggtttc aacgccatct acgctcgcgc gaaaattgaa
   109441 cgtttcgcag gcgatctggg gcagttggtt gctggtcaga ttatgcaatc tcctgccggg
   109501 caaactcctc aagggcaagc attggcagct accgccaacg gtatcgacag taataccaaa
   109561 atcgctcatc tgaacggtaa ccagtggggc tttggctgga acgccggaat cctgtatgaa
   109621 ctggataaaa ataaccgcta tgcactgacc taccgttctg aagtgaaaat tgacttcaaa
   109681 ggtaactaca gcagcgatct taatcgtgcg tttaataact acggtttgcc aattcctacc
   109741 gcgacaggtg gcgcaacgca atcgggttat ctgacgctga acctgcctga aatgtgggaa
   109801 gtgtcaggtt ataaccgtgt tgatccacag tgggcgattc actatagcct ggcttacacc
   109861 agctggagtc agttccagca gctgaaagcg acctcaacca gtggcgacac gctgttccag
   109921 aaacatgaag gctttaaaga tgcttaccgc atcgcgttgg gtaccactta ttactacgat
   109981 gataactgga ctttccgtac cggtatcgcc tttgatgaca gcccagttcc ggctcagaat
   110041 cgttctatct ccattccgga ccaggaccgt ttctggctga gtgcaggtac gacttacgca
   110101 tttaataaag atgcttcagt cgacgttggt gtttcttata tgcacggtca gagcgtgaaa
   110161 attaacgaag gcccatacca gttcgagtct gaaggtaaag cctggctgtt cggtactaac
   110221 tttaactacg cgttctgata acgcgttcgc ctggataaag tcacctgcta tgcaggtgac
   110281 tttaactccc ccacttcacc gaaagtagtt tcctcattgc ttgcctcctg agttttgcaa
   110341 acagcctgat ggcagcttgc cctttcaaat cagtaagtgg tatccacaga caaacctgaa
   110401 gggaaatgca tattttcaag cgttctgctc gtccttctca aagagtttac ttttctgcat
   110461 ttccaggata ctcaaccccc ctggctattg tgcgcttata cactcaaatt aaagataggt
   110521 tctaaataaa tgagcgtttt ttgatagtct atttcattag gtaatatata tttgtaacaa
   110581 atcaatcaac atagaataaa atcatgctac catctatttc aatcaacaat accagcgcag
   110641 cttacccaga atccatcaat gaaaataaca atgatgaaat taatggatta gtacaagagt
   110701 tcaaaaacct ttttaatggt aaggaaggaa taagcacctg tattaaacat ctacttgagc
   110761 ttataaaaaa cgccatacga gtaaacgacg atccttatag atttaatatt aataattcct
   110821 cagttactta tattgatatt ggctccaatg atacagacca tattactatt ggtatcgaca
   110881 accaagaacc aatagaatta cctgcgaact ataaagacaa agaactcgtc cgtactatca
   110941 ttaatgacaa catagttgag aagactcatg atatcaataa caaggaaatg atcttcagcg
   111001 cattaaaaga aatatatgat ggagatcctg gttttatctt cgataagata tcacacaaac
   111061 tcagacatac ggtaacggaa tttgatgaga gcgggaaaag cgaaccaacg gacttattta
   111121 cctggtacgg taaagataaa aaaggcgact ctctcgctat tgtaattaaa aataaaaacg
   111181 gaaatgatta cttatctctc ggttactacg atcaggacga ctaccacatt caaagaggaa
   111241 ttcgtattaa tggtgatagt ctcacccaat attgtagtga aaacgccagg agtgcttcag
   111301 cgtggtttga aagcagtaaa gctatcatgg cagaatcatt tgcaactggt tccgatcatc
   111361 aggttgtaaa cgagctcaac ggggaaagac tgagagaacc taacgaagtt tttaaacgtt
   111421 taggtcgagc aataagatat aattttcaag tggacgatgc aaaatttaga cgcgataatg
   111481 taaaagaaat aatttctact ttattcgcta acaaagttga cgttgaccat cctgaaaata
   111541 aatacaaaga ctttaaggat ctcgaagata aagttgaaaa aagacttcaa aattgccagg
   111601 cgaaatatca aaatgaaatt aatcaactat ctgcactagg tgttaatttt gatgatattt
   111661 aaaattattt ttccagtttt ttgatttttc aacgcctgaa tatc
//
LOCUS       DAJTNM010000003        86242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTNM010000003 DAJTNM010000000
VERSION     DAJTNM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 86242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.003"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..1582)
                     /locus_tag="OO172_000241"
     CDS             complement(<1..1582)
                     /locus_tag="OO172_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5510556.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDKNEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGT"
     gene            complement(1566..1922)
                     /locus_tag="OO172_000242"
     CDS             complement(1566..1922)
                     /locus_tag="OO172_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5510557.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2045..2227)
                     /locus_tag="OO172_000243"
     CDS             complement(2045..2227)
                     /locus_tag="OO172_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510558.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2211..2651)
                     /locus_tag="OO172_000244"
     CDS             complement(2211..2651)
                     /locus_tag="OO172_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5510559.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2651..2947)
                     /locus_tag="OO172_000245"
     CDS             complement(2651..2947)
                     /locus_tag="OO172_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5510560.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2944..3282)
                     /locus_tag="OO172_000246"
     CDS             complement(2944..3282)
                     /locus_tag="OO172_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5510561.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3279..4490)
                     /locus_tag="OO172_000247"
     CDS             complement(3279..4490)
                     /locus_tag="OO172_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5510562.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4492..5064)
                     /locus_tag="OO172_000248"
     CDS             complement(4492..5064)
                     /locus_tag="OO172_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5510563.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5104..6261)
                     /locus_tag="OO172_000249"
     CDS             complement(5104..6261)
                     /locus_tag="OO172_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR5510564.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6561..6785)
                     /locus_tag="OO172_000250"
     CDS             complement(6561..6785)
                     /locus_tag="OO172_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510565.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(6927..7088)
                     /locus_tag="OO172_000251"
     CDS             complement(6927..7088)
                     /locus_tag="OO172_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5510566.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(7098..7403)
                     /locus_tag="OO172_000252"
     CDS             complement(7098..7403)
                     /locus_tag="OO172_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510567.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7400..7678)
                     /locus_tag="OO172_000253"
     CDS             complement(7400..7678)
                     /locus_tag="OO172_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510568.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7958..9706)
                     /locus_tag="OO172_000254"
     CDS             complement(7958..9706)
                     /locus_tag="OO172_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5510569.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9703..10002)
                     /locus_tag="OO172_000255"
     CDS             complement(9703..10002)
                     /locus_tag="OO172_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5510570.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(10008..10241)
                     /locus_tag="OO172_000256"
     CDS             complement(10008..10241)
                     /locus_tag="OO172_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510571.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(10243..10463)
                     /locus_tag="OO172_000257"
                     /pseudo
     CDS             complement(10243..10463)
                     /locus_tag="OO172_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10456..10677)
                     /locus_tag="OO172_000258"
     CDS             complement(10456..10677)
                     /locus_tag="OO172_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510572.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10667..10867)
                     /locus_tag="OO172_000259"
     CDS             complement(10667..10867)
                     /locus_tag="OO172_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5510573.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10876..11622)
                     /locus_tag="OO172_000260"
     CDS             complement(10876..11622)
                     /locus_tag="OO172_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510574.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11680..11868)
                     /locus_tag="OO172_000261"
     CDS             complement(11680..11868)
                     /locus_tag="OO172_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5510575.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(12263..13492)
                     /locus_tag="OO172_000262"
     CDS             complement(12263..13492)
                     /locus_tag="OO172_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5510576.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13741..14862
                     /gene="roxA"
                     /locus_tag="OO172_000263"
                     /gene_synonym="ycfD"
     CDS             13741..14862
                     /gene="roxA"
                     /locus_tag="OO172_000263"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5510577.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14911..16137)
                     /gene="pepT"
                     /locus_tag="OO172_000264"
     CDS             complement(14911..16137)
                     /gene="pepT"
                     /locus_tag="OO172_000264"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5510578.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16387..17523
                     /gene="potA"
                     /locus_tag="OO172_000265"
     CDS             16387..17523
                     /gene="potA"
                     /locus_tag="OO172_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5510579.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17507..18364
                     /gene="potB"
                     /locus_tag="OO172_000266"
     CDS             17507..18364
                     /gene="potB"
                     /locus_tag="OO172_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5510580.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            18361..19155
                     /gene="potC"
                     /locus_tag="OO172_000267"
     CDS             18361..19155
                     /gene="potC"
                     /locus_tag="OO172_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5510581.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            19152..20198
                     /gene="potD"
                     /locus_tag="OO172_000268"
     CDS             19152..20198
                     /gene="potD"
                     /locus_tag="OO172_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5510582.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(20354..21175)
                     /gene="cobB"
                     /locus_tag="OO172_000269"
     CDS             complement(20354..21175)
                     /gene="cobB"
                     /locus_tag="OO172_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5510583.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCHFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(21191..22102)
                     /gene="nagK"
                     /locus_tag="OO172_000270"
     CDS             complement(21191..22102)
                     /gene="nagK"
                     /locus_tag="OO172_000270"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5510584.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(22131..23375)
                     /gene="lolE"
                     /locus_tag="OO172_000271"
     CDS             complement(22131..23375)
                     /gene="lolE"
                     /locus_tag="OO172_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5510585.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23375..24076)
                     /gene="lolD"
                     /locus_tag="OO172_000272"
     CDS             complement(23375..24076)
                     /gene="lolD"
                     /locus_tag="OO172_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5510586.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(24069..25268)
                     /gene="lolC"
                     /locus_tag="OO172_000273"
     CDS             complement(24069..25268)
                     /gene="lolC"
                     /locus_tag="OO172_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5510587.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25530..26576
                     /locus_tag="OO172_000274"
     CDS             25530..26576
                     /locus_tag="OO172_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5510588.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26704..30150
                     /gene="mfd"
                     /locus_tag="OO172_000275"
     CDS             26704..30150
                     /gene="mfd"
                     /locus_tag="OO172_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5510589.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            30293..31255
                     /gene="ldtC"
                     /locus_tag="OO172_000276"
                     /pseudo
     CDS             30293..31255
                     /gene="ldtC"
                     /locus_tag="OO172_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(31338..31595)
                     /gene="bhsA"
                     /locus_tag="OO172_000277"
     CDS             complement(31338..31595)
                     /gene="bhsA"
                     /locus_tag="OO172_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR5510590.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31836..32468
                     /gene="comR"
                     /locus_tag="OO172_000278"
     CDS             31836..32468
                     /gene="comR"
                     /locus_tag="OO172_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5510591.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32530..33069)
                     /locus_tag="OO172_000279"
     CDS             complement(32530..33069)
                     /locus_tag="OO172_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5510592.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(33296..34600)
                     /gene="ndh"
                     /locus_tag="OO172_000280"
     CDS             complement(33296..34600)
                     /gene="ndh"
                     /locus_tag="OO172_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5510593.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(35001..35543)
                     /locus_tag="OO172_000281"
     CDS             complement(35001..35543)
                     /locus_tag="OO172_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5510594.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35566..36591)
                     /gene="nagZ"
                     /locus_tag="OO172_000282"
     CDS             complement(35566..36591)
                     /gene="nagZ"
                     /locus_tag="OO172_000282"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5510595.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36602..37426)
                     /gene="thiK"
                     /locus_tag="OO172_000283"
     CDS             complement(36602..37426)
                     /gene="thiK"
                     /locus_tag="OO172_000283"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5510596.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37407..38048)
                     /gene="lpoB"
                     /locus_tag="OO172_000284"
     CDS             complement(37407..38048)
                     /gene="lpoB"
                     /locus_tag="OO172_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5510597.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(38063..38440)
                     /locus_tag="OO172_000285"
     CDS             complement(38063..38440)
                     /locus_tag="OO172_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5510598.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38443..38802)
                     /gene="hinT"
                     /locus_tag="OO172_000286"
     CDS             complement(38443..38802)
                     /gene="hinT"
                     /locus_tag="OO172_000286"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5510599.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            39136..39960
                     /locus_tag="OO172_000287"
     CDS             39136..39960
                     /locus_tag="OO172_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5510600.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPT"
     gene            39964..41325
                     /gene="fhuE"
                     /locus_tag="OO172_000288"
                     /pseudo
     CDS             39964..41325
                     /gene="fhuE"
                     /locus_tag="OO172_000288"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41385..42818)
                     /gene="ptsG"
                     /locus_tag="OO172_000289"
     CDS             complement(41385..42818)
                     /gene="ptsG"
                     /locus_tag="OO172_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5510601.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(43113..43910)
                     /locus_tag="OO172_000290"
     CDS             complement(43113..43910)
                     /locus_tag="OO172_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5510602.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43921..44925)
                     /gene="holB"
                     /locus_tag="OO172_000291"
     CDS             complement(43921..44925)
                     /gene="holB"
                     /locus_tag="OO172_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5510603.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAALVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44922..45563)
                     /locus_tag="OO172_000292"
     CDS             complement(44922..45563)
                     /locus_tag="OO172_000292"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5510604.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45553..46575)
                     /gene="yceG"
                     /locus_tag="OO172_000293"
     CDS             complement(45553..46575)
                     /gene="yceG"
                     /locus_tag="OO172_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5510605.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46578..47387)
                     /gene="pabC"
                     /locus_tag="OO172_000294"
     CDS             complement(46578..47387)
                     /gene="pabC"
                     /locus_tag="OO172_000294"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5510606.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47507..48748)
                     /gene="fabF"
                     /locus_tag="OO172_000295"
     CDS             complement(47507..48748)
                     /gene="fabF"
                     /locus_tag="OO172_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5510607.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48835..49071)
                     /gene="acpP"
                     /locus_tag="OO172_000296"
     CDS             complement(48835..49071)
                     /gene="acpP"
                     /locus_tag="OO172_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5510608.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(49282..50016)
                     /gene="fabG"
                     /locus_tag="OO172_000297"
     CDS             complement(49282..50016)
                     /gene="fabG"
                     /locus_tag="OO172_000297"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5510609.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGSQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(50029..50958)
                     /gene="fabD"
                     /locus_tag="OO172_000298"
     CDS             complement(50029..50958)
                     /gene="fabD"
                     /locus_tag="OO172_000298"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5510610.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50974..51927)
                     /gene="fabH"
                     /locus_tag="OO172_000299"
     CDS             complement(50974..51927)
                     /gene="fabH"
                     /locus_tag="OO172_000299"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5510611.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(51995..53065)
                     /gene="plsX"
                     /locus_tag="OO172_000300"
     CDS             complement(51995..53065)
                     /gene="plsX"
                     /locus_tag="OO172_000300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5510612.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(53146..53319)
                     /gene="rpmF"
                     /locus_tag="OO172_000301"
     CDS             complement(53146..53319)
                     /gene="rpmF"
                     /locus_tag="OO172_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5510613.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53371..53892)
                     /gene="yceD"
                     /locus_tag="OO172_000302"
     CDS             complement(53371..53892)
                     /gene="yceD"
                     /locus_tag="OO172_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5510614.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            54091..54675
                     /gene="yceF"
                     /locus_tag="OO172_000303"
     CDS             54091..54675
                     /gene="yceF"
                     /locus_tag="OO172_000303"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5510615.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54787..55746)
                     /gene="rluC"
                     /locus_tag="OO172_000304"
     CDS             complement(54787..55746)
                     /gene="rluC"
                     /locus_tag="OO172_000304"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5510616.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            56319..59504
                     /gene="rne"
                     /locus_tag="OO172_000305"
     CDS             56319..59504
                     /gene="rne"
                     /locus_tag="OO172_000305"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5510617.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59701..60654)
                     /gene="flgL"
                     /locus_tag="OO172_000306"
     CDS             complement(59701..60654)
                     /gene="flgL"
                     /locus_tag="OO172_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR5510618.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60666..62308)
                     /gene="flgK"
                     /locus_tag="OO172_000307"
                     /pseudo
     CDS             complement(60666..62308)
                     /gene="flgK"
                     /locus_tag="OO172_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62374..63315)
                     /gene="flgJ"
                     /locus_tag="OO172_000308"
     CDS             complement(62374..63315)
                     /gene="flgJ"
                     /locus_tag="OO172_000308"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5510619.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(63315..64412)
                     /gene="flgI"
                     /locus_tag="OO172_000309"
     CDS             complement(63315..64412)
                     /gene="flgI"
                     /locus_tag="OO172_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5510620.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64424..65122)
                     /gene="flgH"
                     /locus_tag="OO172_000310"
     CDS             complement(64424..65122)
                     /gene="flgH"
                     /locus_tag="OO172_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5510621.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(65175..65957)
                     /gene="flgG"
                     /locus_tag="OO172_000311"
     CDS             complement(65175..65957)
                     /gene="flgG"
                     /locus_tag="OO172_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5510622.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(66095..66846)
                     /gene="flgF"
                     /locus_tag="OO172_000312"
                     /pseudo
     CDS             complement(66095..66846)
                     /gene="flgF"
                     /locus_tag="OO172_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(66866..68065)
                     /gene="flgE"
                     /locus_tag="OO172_000313"
     CDS             complement(66866..68065)
                     /gene="flgE"
                     /locus_tag="OO172_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5510623.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTCAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIVATT
                     QNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQ
                     SSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQIL
                     NTRVNLR"
     gene            complement(68090..68785)
                     /gene="flgD"
                     /locus_tag="OO172_000314"
                     /pseudo
     CDS             complement(68090..68785)
                     /gene="flgD"
                     /locus_tag="OO172_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(68797..69200)
                     /gene="flgC"
                     /locus_tag="OO172_000315"
                     /pseudo
     CDS             complement(68797..69200)
                     /gene="flgC"
                     /locus_tag="OO172_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(69204..69377)
                     /locus_tag="OO172_000316"
     CDS             complement(69204..69377)
                     /locus_tag="OO172_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HCR5510624.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69532..70191
                     /gene="flgA"
                     /locus_tag="OO172_000317"
     CDS             69532..70191
                     /gene="flgA"
                     /locus_tag="OO172_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5510625.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            70267..70543
                     /gene="flgM"
                     /locus_tag="OO172_000318"
                     /pseudo
     CDS             70267..70543
                     /gene="flgM"
                     /locus_tag="OO172_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
     gene            70548..70964
                     /gene="flgN"
                     /locus_tag="OO172_000319"
     CDS             70548..70964
                     /gene="flgN"
                     /locus_tag="OO172_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5510626.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(71004..72539)
                     /gene="murJ"
                     /locus_tag="OO172_000320"
     CDS             complement(71004..72539)
                     /gene="murJ"
                     /locus_tag="OO172_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5510627.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSHLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72706..73572)
                     /locus_tag="OO172_000321"
     CDS             complement(72706..73572)
                     /locus_tag="OO172_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5510628.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(73574..74221)
                     /locus_tag="OO172_000322"
     CDS             complement(73574..74221)
                     /locus_tag="OO172_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5510629.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(74232..74816)
                     /gene="rimJ"
                     /locus_tag="OO172_000323"
     CDS             complement(74232..74816)
                     /gene="rimJ"
                     /locus_tag="OO172_000323"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5510630.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            75052..76260
                     /gene="mdtH"
                     /locus_tag="OO172_000324"
     CDS             75052..76260
                     /gene="mdtH"
                     /locus_tag="OO172_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5510631.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEYR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            76324..76971
                     /gene="grxB"
                     /locus_tag="OO172_000325"
     CDS             76324..76971
                     /gene="grxB"
                     /locus_tag="OO172_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5510632.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYHDNMAKQTQINLLSSMAI"
     gene            77105..77665
                     /locus_tag="OO172_000326"
     CDS             77105..77665
                     /locus_tag="OO172_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5510633.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77771..78817
                     /gene="pyrC"
                     /locus_tag="OO172_000327"
     CDS             77771..78817
                     /gene="pyrC"
                     /locus_tag="OO172_000327"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5510634.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78891..79136
                     /gene="dinI"
                     /locus_tag="OO172_000328"
     CDS             78891..79136
                     /gene="dinI"
                     /locus_tag="OO172_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5510635.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            79426..79680
                     /gene="bssS"
                     /locus_tag="OO172_000329"
     CDS             79426..79680
                     /gene="bssS"
                     /locus_tag="OO172_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5510636.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79795..80913
                     /gene="solA"
                     /locus_tag="OO172_000330"
     CDS             79795..80913
                     /gene="solA"
                     /locus_tag="OO172_000330"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5510637.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80961..81074
                     /locus_tag="OO172_000331"
     CDS             80961..81074
                     /locus_tag="OO172_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5510638.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            81335..81901
                     /locus_tag="OO172_000332"
     CDS             81335..81901
                     /locus_tag="OO172_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5510639.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81905..82480
                     /locus_tag="OO172_000333"
     CDS             81905..82480
                     /locus_tag="OO172_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5510640.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82522..83574)
                     /locus_tag="OO172_000334"
     CDS             complement(82522..83574)
                     /locus_tag="OO172_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5510641.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83799..84719
                     /gene="lpxL"
                     /locus_tag="OO172_000335"
     CDS             83799..84719
                     /gene="lpxL"
                     /locus_tag="OO172_000335"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5510642.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84891..85088
                     /locus_tag="OO172_000336"
     CDS             84891..85088
                     /locus_tag="OO172_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5510643.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            85112..>86242
                     /locus_tag="OO172_000337"
     CDS             85112..>86242
                     /locus_tag="OO172_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5510644.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT        20697 a        22920 c        21231 g        21394 t
ORIGIN      
        1 atgtaccaaa cgccatcagc gccgccacgc tcgggtctat cttgttcggt gattttttct
       61 tgttcggctt gatattggcg ttcgcgtcac tctgcatcac gacgttactc atcgaccagg
      121 acaacaccgg atcgccacga tgcacaatca ccctgcggtt aacaaaaact tcgaacgatt
      181 tcgccgccgg actgaatctg aggtaggttt gcgggaacgg ctccacctca aaaccagccc
      241 cctgtaattg cgtcctgaga tgcgtggcgt tccacgtatc aaagcccacc agcctgatat
      301 taaatttctc cgcatcctgc atgatgtcat ctctgatccg gtcgtaatca atgcagtcac
      361 ctggcgttgt gcgtatccag cccgctttag cccactggcg atagacagcg cggtttttat
      421 tggcggggtt ctgtagctgg aactccggca gataatgacg ggaaatcagc ataatggttt
      481 taccgaccgg aaaggcatag catacgctgg aaatatcgct ggttgatgat aagtccagcc
      541 ccgcgtaaca ctcctgaccg tgtaaatctt cctccgtgaa cgttccggca cactcagccc
      601 atgcaccatt acccatccac ggggtagtcc cctgacacca gatattaaat cgctttgtca
      661 tcatctccac ccactgcgac ggaatacccc gcgctttctg gatggttgag gccagttttt
      721 cacgatccac ggaaacatca atattagggt tcgcctttat ccacatcgcc ggatcatcaa
      781 cctcgttttt gtcgtccagt tcgtaaatca gtacaaaaat tgaatcgttg acctcttcgc
      841 cgtccaggat ctggcagcaa taatcatagt gctgtttaca ggctgaaacg acgttactgc
      901 ccgatgtggt aatggcaaat aacagccctt cgggacgtgc gcccatcccc agctcaagcg
      961 cggaataaac gccgttatcg gggtgtaggt ggtattcgtc cacgatggaa agactggggt
     1021 ttgtcccctc gatggttgcc gcttttgctg ccagcggctt taacaggctg ttgcttttcg
     1081 ggtgtatcac cttatgcgcc tgaatattca cccgcctgcg taacggtcgg gataaaaggc
     1141 acatctgacg cgcatcatca aacacgatcc gcgcctggtc acggctcacc gctgcggtgt
     1201 agatatcctg ctgaccgttc tccataatca gaaaccagtt agccagaatc gcggcggtcg
     1261 tggatttcgc atttttgcgc ggcacttcga taaaggcgct cgtgtatttg cgccgtccgg
     1321 tggccttaac cttaaagccg aggatgcacg caaaggcgaa ctgctgccac ggctccagct
     1381 caatgggtct gccacgcatc ggtcctttta cgtgcgggca caccctggaa aaggcaataa
     1441 accgctccac aacctcacga tcgaacatgt aaagggggct tttaaggtcc gaaaagtacc
     1501 gtttaacagc ctgttttaac cgtttacagg ccggaatttt gcccgttttt acgtcttctg
     1561 cgtacttatt ccaggcggtc aagctcgtcc tcttcttctg tttccggtgg atttttacgg
     1621 cggcttatcg ggtcaaaacc gagcaaggag gccattttta tcatcactct ttcagcgtcg
     1681 gattttgcgc ttaatgcggg gtttctgctc tcgccgccct gactgttaac aatgctgaac
     1741 ccgcgcgccg caaggtctgc aacggctttc cggtaaatgg agtagttgac gcaatacagt
     1801 tccagattgc tccagtcggc gggggtcagg tctccccgtt ccgcaagctg ccgcgatttt
     1861 tcccgccact gcttcacggc gatatcatcc aggtaggcgg gggctttcgg tggtcttgcc
     1921 atgcttattt ttcgtcagat tatttttcaa aaaattcccg tgcataaaaa tttggggagg
     1981 cgttcggtgc ccggcggggt cgggtttgtc ctgaaaacgc cccccacccc gtcatacagc
     2041 ctcatcagcg attgcggaaa cattccataa cctcgcggtc acggtcggtt aatcgcttcg
     2101 ctgtgatgcg ttctgcgcgt cctgacgctt tatctttatg ccctgtttcc tgtgtcttcc
     2161 atgcgtcacg ctgccttata agtccacgga taaggcggtt ttgttcccgc tcattcatca
     2221 gcgtcataca tccagttatt gcggttagca gcccgttctt cctcttcacg aaatccacct
     2281 gcggcacgct tgctttttgt tgccggatca agccatttcg ttttctggtt atgacacgcc
     2341 tggcacaatg gttgatggtt ccattcgggc cagaagagaa catcatcacc gccattaatc
     2401 gggataatgt gatccaccac cacggcgggc gtatatatcc ccttctcaag gcatcgcacg
     2461 cataacgggt ttttactcag atacatggcg cggtatttgt cccactgtct ggaatatcca
     2521 cgcgcgcggc ggtgtcctcg tctggcatcc tctgcacgcc atgccgcccg cctgtgctcc
     2581 tcacacttgc cggatttaac gcgcctgtta cagcctggtt ctgtgcatcg tcttaatggt
     2641 tgccacggca tcagtacacc cccacatcac ggtaagccgt ccagagtgcg cctatcgtca
     2701 tgggtacgcg tgtttttgcg ttatccgcga caatctggcg attttcatac aggtgagcga
     2761 taaacatcat gcagccaatc tttatggctg gcgtgaacgc cagcccgtca tcaaagcgcc
     2821 tgcctatgtg tatctggcac gcctcaagcg atgcggcaat gtatccgctg attaactggt
     2881 cttcatcgtc gccatcgatg cggcaatgga gtttcacttc ttccagggtg ataagttctt
     2941 ctgtcatttt tccgcgccct cacgacaaag aatttcaagg cgtgtcctgg cggcatccgg
     3001 cagcggctgc ccgatgatat tcagtacacg ccccgccagc ggccccgtac tgacctttat
     3061 ccggctggtg gcgttgatgt ccttccggta ccgtatccag atccttacgg taccaatcgc
     3121 cagctctgcg cccgatgaaa tggcctcctt gctgctgacc atgttcacgc ttgcccagag
     3181 tgtgtgaccg tcctcccacg tttcgagtat ttcgccggtt atatccctgg tctgtttcag
     3241 ggtctgaatc gttatcctgt cacgcagtcg ccctatgttc attccggttt ttctccctcg
     3301 ctgattttta cttcctgttt ccatgcctgg ctgaactcat caccaccatc acgcggtgac
     3361 agcccttcac gttcgcgggc ttcgttcgga cacataacgc cggattttat gccgcgctca
     3421 taggtggcaa agcgttcgcc aggtgtggcc cgtaacaggt ccgcgctgtc aaactccacc
     3481 tgataccgga taccaggtac aggcgaggcc accagcaagg cattttttat ctgctgctca
     3541 aagttcgcca gccacgggcg catcgtcatg gtgagaaatg cgcggcttgc ctcgctgaag
     3601 ttgctgtagg tgctgttgct gtattcctgc aaaaaaatcg gcgagacgtt gaacattcgg
     3661 gcgatgtctt caatggtgaa gcgacgggag gccagccatt cggcatcctg attactcatg
     3721 cccagctgct gatagctcat ccccccttca aggatgggcg tttttccggc gtttctggcc
     3781 cctttgtagc gttccagtgc ggctaatgcc tgtttgccct tcacgccgtc cagccattcg
     3841 cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc gccgtggcgt
     3901 tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga gcgccccata
     3961 aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata gttgcgggtg
     4021 tttccggtgt aggcgtcagt gatgcagtag cgccagttat gatcacctgt ctgctccacg
     4081 ttcaccgact gcggcgggta aggatgtaaa gccgccggaa aaccatcacg cccccactga
     4141 ataaccgcat aagcattacc acttaacagg cagtggcgga tcatcattcg cttaaactgg
     4201 tagggtgtct gccacgcgtt cgggcgctcg ttgaggatgt gatcgaccgg atgagaatca
     4261 agccattcgc gggcctcctt ccccttctca ttgcgtacca ggtacaggta acacggcatg
     4321 gtagccaccg cctcagagat gaccgtgacg gcgttcatga cggcggagag tgattccgct
     4381 gtcccagatg atacgtactc acccgccccc gtgttcgatg tgccggccag cgccataaat
     4441 tcatcaagcg tcatgctgcg ctgctctttt tttcgtctga aaggccacat atcacacccc
     4501 cgctaaatcc gcccaccagc ggcgattatc ctcacgcggc attttttcgg ggtgctgctc
     4561 atacagggaa cggcgggcca gctccacgcc ggaatcgggg taagccggta ccgatgtaac
     4621 ggtaatttcg tacagttccg ccaccagcac ggtgcgcacg catggatctg ttgtggtatc
     4681 ccatacatcc ttacgggagc gaaagctaaa gctcatcccc gagatgtccc cacgcttaac
     4741 cagttcgata acgtcgcgcc ctgtactggt atccggtggg gtcagttcaa agcgtaaccc
     4801 tgtttcgtcc tcttccagtt tcagcgtgcc ggaacgggtg cgccccagta acatgctgtg
     4861 gtcatgctca tacaggccgc gaacgtcatt acccgccgca agccattcag taaacgcccc
     4921 ccgctggaat ttttcgtaaa actcacccca taacagttct gaaagcttat cccagcgaac
     4981 aacgtagccc gtcagcgtac cggctccgct ggtggtgatt tccgatgacc ggatttccat
     5041 actcttcata attttttcac ccataaaaca ctaaaggggc tttttagccc cttctgtatg
     5101 ctgttaatcg tcgtcttccg gcagttccag aatcttgatc gcgttcgaat ccaccacgcc
     5161 accgcctaaa tatttttgcg tgaaaatttt gatgaatcct ggttctgtca ggttgtccgg
     5221 tctggtgcga acacctgttt cgtgatcaac aatgtagtaa ccgcgtttga agtcacccag
     5281 ggcaataacg ttatcaggca taaactccag atattcgacc ggaaggccca gcaacgtatc
     5341 aggatcaccc gcctgtaaac ggtcgcgcca gatataatca ccgttcgcgt tcttcacctt
     5401 ctgaagtttt gccgccgtcg tggagttaac cacccagacc gcgtttttgc ggtatttttt
     5461 acgtaatgca aatttcaggt cgatcagcgg gtccgcagat gtccacgcca gagattcgga
     5521 aggtttaatt acctgcaacg taccaaaatc acgctctttg tcgttcttct ctgcacgggg
     5581 tacggataaa aaaccttttg cttttttgtc accgtcgccc acaaccagat cgctttcttc
     5641 ggtttccgtg aaggtgtcgc caatctcacc cgtcagccat gaaaggatgt ccacatcgga
     5701 aaaatccacg atttcctgtg tggtgcgcgg gtacgcatag accggataca gcttaatgct
     5761 cacctcgtta atctgcgggg tgctggtctg ttcgcgtgcc ttaccctctt caccgtggtt
     5821 aacggtcgca ccgccagcgg aaacaagctg cttaaactcg ttgctgctga ttttcttcac
     5881 ggtacagatg cggcgcatag tggattcatc cgccagcatt cgcatgattt cggtgttcag
     5941 ttcagggata acggtataac caccatcagc gggaacgcct gtacttaatg cgcgggtttc
     6001 accggtcaga atgtagttgc gtagttctgc ggggtcagtg gtctggctgc ttttacctgg
     6061 cttgctgcgc tcttcgtctg caatggcttc aaggcgggaa atgtctttat cgagggattc
     6121 agctttagcg cgtaattcgt caaattttgc gccctcagca tcgttaagac tgcggttttc
     6181 tttttcagcg ttctccagca tgtcgcgcat ctgattttta atggcggttt tctgctggcg
     6241 taattcgatt attctctgca taaaaaagtc ctggggttaa gtaaggaact ccaggacgcg
     6301 gcaaacactc aaccgttttt cataaggaaa tcagtaatcg caccgatcgt tttcccgcct
     6361 ggtaatgaat attggcgagc acattaacag gcgggaaagt ggccccagcg tcctggcacc
     6421 acgggcgaga ataatcatga ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa
     6481 cgtggaacaa ccacgaacaa ataatttaca aaaaataaca aaaagccgga ttgctccggc
     6541 tgtatgtgct gtatgggtgc tcagtctctg atccctttca gaaattctat caatgcatct
     6601 gtctgttcag gatttactgc cagcatttca ccggatagcg cgcaacgaac aaaaccatga
     6661 tgatcctttt caatcagcgc ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc
     6721 gtctccatgt tgtcagcatg gtatttatca cgctgtttaa gtatctcatc aaatttcatc
     6781 ggcattgcgt tttcctctgt tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg
     6841 acgaaaagcc agtagtgctc cataccgttt caaatgacgc aaaaaaagcc ggattgctcc
     6901 ggcttcgtgg tgtggttctg atattcctac tctgctaatt tgctcagccc catgtctgcg
     6961 taagtgcgat ttactgcatt tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg
     7021 cccttgctgc tctgttttac cagcgggtcg cccaccagcc gcccgtaagc tgcaatttgt
     7081 gctgtcatat tgccacctca gtgaagcgat ccggcattgt tctttttcat ctcttccatc
     7141 attttttcag tgagcattac gcactgaaac cccatgtttt tgatgtctac gggtttcatg
     7201 cctttgtcag tggatatcac aggcagaata tcctgataca catcgcggtc agggtaacta
     7261 agcacacaaa gcgcccatga tgccacgggt gaggatcctt cgtctgtttc aacccaccag
     7321 cctggtgtag ctgcgcttat ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt
     7381 tcaccaaatt ttggttcgct catgattcca cctctccggt tttaacgttg attgttgtta
     7441 cctgttccgc ttcggcaatc tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg
     7501 tcatgaatgc gcttatcagt tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt
     7561 ctatcgttct gattgccacc tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg
     7621 gttctgttct gtctctgcgt tttttgagtg atttattaaa tttccctgaa gtgtgcatat
     7681 ttatttttac cccctcgttt aaaaagtttt gagttgtgcc tccccttgtc taccttagtg
     7741 gccctcatgc cagtaatggc gctgctttca gcggggtaga gtgcttttat ccactatcta
     7801 ccccgtgtct acctccctgt ctgattcagg taaaatcagg tagagagggt agatagtggg
     7861 tagacagtaa aaaaaggcta cctacctaac ttaatgcact gaattaaatg tatttttctt
     7921 tactcaggta gatagggtag acagcaatta caaaaaatta taaaaacgcg tcgcactcgt
     7981 ctgttgttat tgcgttagtc tgcgttactc ccttaacttt ccgcgtaata tattcatgtc
     8041 cgtaaacttt cgcggctggc ttcatagcct tgccaaagtc atttacgttt agcggtttgc
     8101 tcctgcctgc gtacgccata aacgccagat agacgcggta aaggctgttt ctggtcgtgt
     8161 acttcactga atcgccaccg ccgcccatca tcaggccgcg tgcttcctcc agaaaattca
     8221 ggaactggca aaactcaata accggatccg tctgttgctt tattgccaga gcttcatcac
     8281 cgtcacgctg ttccagtagt aaagcccgtg ctttctcaga gtcggtaaag ttcgccagca
     8341 atcggcggat aatgacaggg atttcagccg caatcttttc cggtagctcc ctgtcttttt
     8401 cggcctcact aacgatattg tcgaaacgga aaatcacgcg acgacgtgcc acacctccgg
     8461 cccgttcggt gaatatcatc gggttattgt tggtcgccag caccaccgcc ctgattacag
     8521 ccgtgaaacg cttttcgtat ttcgggttaa tttccacggg gtcaccgccc gtgattttct
     8581 tgatgcccgt tccttcgcct gtatatttcg gctggtcagc caggacgata agacgactcc
     8641 cgacaacctg cggacgtcca ccagcatcat caagcgatgt catttcagcg cttaccgtgt
     8701 tctgtttccc tgccagaagg ctggctatgt gtgtgaatgt acttttaccg ctcccgccgt
     8761 ctccggtggc ctcaataaac atctgccagt cgtaccggtt cgccataatc atgtacagcg
     8821 cggcacatat acgcatcatc ttgcgctggt cttttccggc tgcgtgctca agccatttat
     8881 gaaagtttag cgcgttatcg cggatgttct cccctggtgc tggtggcgtg tactcaatgc
     8941 cgttgtgcgt ggtgatccag ttctccggcg tgtgcgggga aaattccccc gttttcaggt
     9001 caagcgcacc attggcgaac ggcagcaaat cgccggacgg ctcgcccatt ggttcggcaa
     9061 taacttttaa cgcttccacg gcgttattga ttacgcgctt gctgaaagtg gccctgtgct
     9121 ctgaatagat cgccaccatt tcgcggctaa gttccattgt gctgaccgga caccataccc
     9181 cgccgcgcca tacgtgaacg atttcacttt cctgatgcac acaaacgcca tcaaagcgct
     9241 cggcaagcag ctgcgcgcgc tcactgtctg ccatttgtgc cagttgcgta tttttctgct
     9301 ccggcatggt aagccctgcg gcaatatttt cacgctcagc attcagatag cgccgccagt
     9361 tttcacactt ctggacgtgc attccttcgg ggtaaaaatt tgcgtcctga atatctgccg
     9421 ccgccagctt ctgaccaatc tttttggtct ccaccaaatc cagttctccg gcctggtaca
     9481 gccttacgcg tttttttcca tccggaacaa ttttcagtgc atcaagttcg gcaagctgat
     9541 ttggcccaag ccacacaggc ggcacattat cgccggatgc ggggccatcc tgctcctgcc
     9601 attgtttcgc gtgcgaccag gcatcactac ccgcaaagat aattacctct gtgtctttgt
     9661 gttttattcc gcgtggctgt ttttttacgt tcggtgccag tttcattttt tacccctgaa
     9721 tacgttaagc atctttttta tttcctgaat attggcgcgt gctttctccc tgctggttgg
     9781 tttactgcgg ggcgctgcct gtaccagcgt gaaatcacgc cggaattgat aaacaggcat
     9841 cacgcaatca tattcgtaac cttcacggcg gtaggtgatg cgccgttctg ccacgccttt
     9901 aatcgttacc atgccgccgt actggtcgcg gtaaatatca ccgcgcgtaa atttagggtg
     9961 agtgttgcca ctggcagtta agccagaata tttaagtttc attattttta ttctccggtg
    10021 tgctgttctt tatatctgtc gtgcaataga tctatttctt gcagttccat tattacaggc
    10081 tcaagaagcg ttattaatgc cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg
    10141 ccaagtgttt caagccatat gcgcaatatt tccagcatgt tttcactgtg agaaagtgca
    10201 agaaatgcgc ggtctattgt ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt
    10261 ttttcttaaa ataacctctg tcacgaaatc agcataatcg gcggcgatat tcagtacatc
    10321 aagcccgttg atttatattc tctcgtggaa agtaagaaaa aagccgctct aataagttct
    10381 ggcattgacg aaagcgcatc agccgcatca tcaggaacgc cggaaaattc ctgtttcagg
    10441 gaattaaaac gatcatcacg cataaccccc cccattttca caatcggcaa caagaatatt
    10501 tttagcgtca ttcagtgacc gcgttgcgat gtatcggata cattccaggg cgaactgtgt
    10561 ccggtctttc tgttcttctc cctgcgcaag ctctgccgtg cattcaatat caataagcgc
    10621 gtgcatcagc gtagtgagtg cggcggctgc tgcgttcggt gtggttttat tgtgcatagg
    10681 tttccccctg acgaatacgg gcggcgaata cagcaacaca accggacggg caacggctac
    10741 gcgcttcgcg ttccgtccaa gcggttacgt ggatgatttg ggattctccg gtactcagtg
    10801 ccagaaaacg ccacacaaag gccgtttgtg tgtgtacaag gtgtggtata tgatttacgg
    10861 caaccataac ggctcctagt ttacgttgtt ggttagaagc cccgttactg ctcctacagt
    10921 gcggggcttt gctttttgtg ttgattgtgt gtaatgtgtc attacacata aatacattac
    10981 atgaggtgta attaacgtgt caacacacaa aaacgaaagg cgtggtaatc ctccgttcca
    11041 gttccgttta gatcctgact tacgggaaaa gatggaaaag gcacaacaac aggacggcga
    11101 tgattctctt gctgcctgga taaagcgaat aatccgcaaa gaattacagc aacgcggcat
    11161 agaacccaaa ggataaaaac tatcagcgcc gtggtgtgag gtactacggc gcattgcttt
    11221 acaggcgcac acaatgacca acgaagaatc aaccgaaaaa acatcacctg cacgaaaaag
    11281 acgacgcaga aagaaagagc atgaatcaga acgattcgcg ccgtgttctt ttgcccttga
    11341 gaagtacctt aaagaatact catcaaagga gagaacttcg cagttatggc aacgcactga
    11401 accagactga ttgcattgcc caccagcctg atagcggcta tcatccccgt gcttatgttt
    11461 gggatcacac acacaaacgg cgcagcgggt tatctgttca gaaaggcggc tccatttcgg
    11521 ggccgctttt tttatgcctg aaaaacccca attttgtggt tttccagttt caccagggcg
    11581 aacgaatccc cgcccacgtt ctggcgtata ttcaatcttc atggttatag ctctgtgttc
    11641 agatgattga tgtgtggcgg ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct
    11701 atccttccag gccagaacct cggataaaga ccagccaacg gaacgaccgc caagtttacg
    11761 acgtgatggg aattgtccgg ccttttccag gcggtagcgg catgagcggc taaggcctgt
    11821 tagcttttcg cattcttttt cacgtataaa ccgatcagtg cttaacacta ttgccccctt
    11881 tcgtttctta aagagttatt tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg
    11941 atgattcaga gttggcaaat gttgaggccg tatggtttac agaaagagga ataatcagga
    12001 taaaatcatt taaattcatg ttaatacaaa ggcataaaat attgtttcat gccttttttc
    12061 tcgctattta aagagtgatt cgctagtgta taaaaaacca gtaagacgtt aaaaatcagc
    12121 tacttataaa tctgtacact ttttcacctc ttgttcgtag ttgttccgca ttgttgctca
    12181 ttgttgctca ttgttgctca ttgttgcacg ttgtatctgt tcgcatatcc agtatgcgca
    12241 tactgaaaaa acacgaaaaa aattattttc ttctggctac tggtagcgtg gttacgtttt
    12301 catgtgttcc cgccagtatc cctaaccgct ccgtccacat atccagcgca ttgcgtttag
    12361 catccagata acgggaatga ttataaactc gctgcattcc tggcatctgg tggcctgtaa
    12421 gctgctccac gacgtgagga tccacgccta aatcgttcag catcgttgta aaggtgcgcc
    12481 ggatgtcatg tagtgaccag tgagggtgat ttagcctcct gtgcgctaat ctgccgtact
    12541 gcgacacgct tgtttcctgt ttcacttccc ccagcaataa gcccgtgtgc ctgttctgct
    12601 ccaccagctg cgtgacgaac ggcagtattg cttccggtat gggccggaat attgccacct
    12661 tcgttttgct gtgttctttc ggcacggtcc agagcatttc ggtaaaatcc cactcgctga
    12721 tctccgataa cctcagttct accgtccggc atccgaacac aatcaggagg cggattaacg
    12781 cgatgtagta gggggagaat atttttttgt ccagtgcctg caataattcg cccagttctt
    12841 tggtgcttaa gacacgctcg cttatatccg gttttttccc aacgtccgcc acactcatat
    12901 catcaagaac gttgctgatt gcatagcgcc gcctccggca gaacttaagc gcctgtttgc
    12961 acgtctgtag caagaatccg gcagtaacag gcgttcgctt tgccacctgg tcaaaacagg
    13021 ccagccagtg ccgtagctcg catttatcca gcggcatagc accaatgtgc tgtattacgt
    13081 gattattaag gcgctttttc agggcggcat aatccacgcg gttttccttt gcgtacgact
    13141 caagccagta ggtgagcgca tcgccaaccg ttaccggctt taacgcttcc tgtacggtgt
    13201 aattaagctc atgacgtgga tttttcccct ctgccagcca tgcgcgacac tgggcggctt
    13261 tttccctggc tgatttcagg ctcagatcag gataatttcc cagcttaatg cgttccggtg
    13321 gtgcccccct tcccgttccg gccctgtaag tgaaatacca ggttaaaagg ccactggttg
    13381 aatgcctgac gctcaggttt ccaccgtcat taagaaaggc tgttttttgg gcgggtgtgc
    13441 cgttgatttt cctcagctgt gtatcgctca gtttgttaag tgctctgctc ataattttga
    13501 ttccggtcat cacaattaac tacaccatta actacaccga tcgttgcaca aagggctaca
    13561 acgtgaaaca agctggaaca aggaaaatca caaatctgtt tataatcaaa aacataatga
    13621 acaatctgaa acattatgaa acggcaaaaa acactaattg ataaaatatg tccatacttc
    13681 acgcattacg ttaagcatcc gttataatcg gttgcagata ccagcctgtg gatgcttaac
    13741 atggaatacc aactcactct taactggccc gattttcttg aacgtcactg gcagaaacgc
    13801 ccggtggtgt taaaacgcgg ctttaataat tttattgacc cgatctctcc agacgagttg
    13861 gcgggtctgg cgatggaaag cgaagttgac agtcgactgg tcagtcacca ggatggcaaa
    13921 tggcaggtca gccacggccc gttcgaaagc tacgatcatc tcggtgaaac caactggtca
    13981 ttactggtac aggcagtaaa ccactggcat gagccgaccg ccgcgctgat gcgaccgttc
    14041 cgtgaactac cggactggcg tattgatgat ctgatgattt ctttttctgt acctggcggc
    14101 ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca ttcagggtac cggacgtcgt
    14161 cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact gcccacatcc agatctgtta
    14221 caggtcgatc cgttcgaagc catcatcgat gaagagctgg agcctggtga tattctttat
    14281 attccgccag gattcccgca tgaaggctac gcgctggaaa atgcgatgaa ctattccgtg
    14341 ggttttcgcg cgccaaatac gcgggaatta attagcggat ttgccgatta tgtgctgcaa
    14401 cgtgaactgg gcggcaacta ctacagcgat ccggatgttc cacctcgcgc tcatcctgcg
    14461 gatgttctgc cgcaagagat ggataaactg cgtgagatga tgctcgaatt gatcaaccag
    14521 ccggaacact ttaagcaatg gtttggcgag tttatatccc agtcacgtca tgaactggat
    14581 atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct acgatgcact gaaacaaggt
    14641 gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca ttggcgacga cgtgtatgcc
    14701 aatggtgaga agatcgattc cccgcaccgt ccggcactgg atgcactcgc cagcaacatt
    14761 gcgctgactg cggagaattt tggcgatgcg ctggaagatc cgtcattcct cgcgatgctc
    14821 gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt aagtttggat ttaagccgga
    14881 tgcagcatca catgccgcat cccttttcgc ttacttccgt tgcgccgtta actcggcaat
    14941 acggacgatc acctgcaccg ctttttccat accttccaga atcacaaact catgcttacc
    15001 atgatagttg taaccgccag tgaacaggtt cgggcaaggt aatcccataa acgacaactg
    15061 cgcgccgttg gtaccgccgc ggatcggttt cagttccggt tcaatatcgc agtcacgcat
    15121 cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc actttctcgc gcatattgta
    15181 gtaactgtct tcaatcacca attcaatgta gcaatcagga tgtaaccctt tgcccacttt
    15241 tttggcgatc tccatcattt tacgtttacg cgcttcaaac tgtttacggt cgaaatcacg
    15301 gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc atgctcgcca ggtgatagaa
    15361 accttcatag ccttctgtca tttccgggct ttcatccgcc ggaacttccg catgaatacg
    15421 tgccgccagc gacagcgcat ttaccatcac tcctttcgcc gtgcccggat gaacattgtt
    15481 accgacaatt ttgatattga ccgatgcggc gttgaagttt tcaaactcca gttcgcctac
    15541 gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag gcatcaacat caaaatgttt
    15601 cgcccctttg cccacttctt catccggggt aaaggcgacg cgaatatcac catgcggaat
    15661 gtttttctgt tgcaataccg ccagcgcggt catgatttct gcaatacctg ctttgtcatc
    15721 ggcacctaac aaggttttac catcggtggt aatcaacgtc tgacccagta gctgatgcag
    15781 caccgggaac ataaccggtg ataaaacttc atcgccgata cccagcgcaa tatcgccacc
    15841 gcgatagttt tcaacaattt gcggattaac atttttgccg ctgcaatccg gtgaggtatc
    15901 cacatgagaa ataaagccaa tcgccgggat atcgccaggg acgttagccg gtaacgtcgc
    15961 catcaaagtg cccttctcac ttaaggtcac attgataagc cccatttctt cgagctgctc
    16021 tttcagcaga tgcaataact tccattggcc ttccgtgctg ggaacctgtc tcacccctgc
    16081 ttttgattgg gtatccagag acacgtagtt caaaaatcgc tcaagtagtt tatccatgta
    16141 gtcaccctca ctttttgtga caacattatt aagaagcaag aaaagacaaa tattgcgtca
    16201 ggtcactttt acccctgcaa gcgggaatat ttatcagcat taccttaatg aatataaagc
    16261 taagccagta attcacaaca aagattggcg attcaagcgg tttgccgtag aattaccgcc
    16321 ctcattaaga gtcaccaagg tggttaacca caaaccccgc atcggtaagc catccgttgc
    16381 gtttacatgg gacagagtaa aaaattgaat aaacaaccga gttcgctttc accgctggtg
    16441 caattggcgg gaattcgcaa atgctttgat ggtaaagagg tcattcccca gctggatctg
    16501 actatcaaca atggcgagtt cctcacgctg cttggccctt ctggctgcgg taaaacaacc
    16561 gttcttcgcc tgattgcagg tctggaaact gttgattccg gacgcatcat gctggataac
    16621 gaggacatca cccacgttcc ggcggaaaac cgctatgtga acactgtttt ccaaagctat
    16681 gcacttttcc cccacatgac cgtgttcgaa aatgtggcct ttgggttgcg catgcaaaaa
    16741 acccccgctg ctgaaattac gccccgcgtg atggaagccc tgcggatggt gcagctggaa
    16801 accttcgctc aacgtaaacc gcatcagctc tctggtggtc aacagcaacg cgtcgccatt
    16861 gctcgcgcgg tggttaacaa gcctcgtctg ttgttgctgg atgagtcgct ctcagcgctg
    16921 gattacaaac tgcgtaagca aatgcagaac gagctgaaag cgttacagcg taagcttggc
    16981 attacattcg ttttcgtgac tcacgaccag gaagaagcac tcaccatgtc ggacaggatt
    17041 gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc cgcgtgaaat ctacgaagag
    17101 ccgaaaaacc tgtttgttgc cgggttcatt ggcgaaatta atatgtttaa cgccacggtg
    17161 atcgagcgac tcgacgagca gcgcgtacgc gccaacgttg aaggccgcga atgtaatatc
    17221 tacgttaact tcgccgttga accggggcaa aaactgcatg ttctgctgcg cccggaagac
    17281 ttacgtgttg aagagattaa cgacgacaac cacgctgaag ggctgattgg ttacgttcgt
    17341 gagcgtaact acaaaggcat gacgctggag tcggttgttg aactggaaaa tggcaagatg
    17401 gtgatggtca gcgaattctt caatgaagac gatcctgact ttgaccactc tctcgaccaa
    17461 aaaatggcca ttaattgggt agaaagctgg gaggtcgtac tggctgatga agaacacaag
    17521 taagttccag aatgtagtga ttgtcactat tgtcggttgg cttgtgttgt ttgtctttct
    17581 gcccaacctg atgatcattg gcaccagctt tttgacccgc gacgacgcca gtttcgtcaa
    17641 aatggtcttt acgctggata actacacgcg tctgctcgat ccgctctatt ttgaagtgct
    17701 attgcactcg ctgaatatgg cgctgatcgc caccctcgcc tgcctggtgc tgggctatcc
    17761 atttgcctgg tttctggcga agctgccaca caaggtgcgt ccgctgctgc tgtttctgct
    17821 gattgttccg ttctggacca actcattgat tcgtatctac gggctgaaaa ttttcctcag
    17881 caccaaaggc tatctcaacg agtttttgct ctggctgggc gttatcgaca caccaatccg
    17941 tatcatgttc acgcccagtg cggtgattat cggtctggtt tacattctgc tgccgtttat
    18001 ggtgatgccg ctgtactcca gtatcgaaaa actggataag ccgttgcttg aggcggcgcg
    18061 cgatctcggt gccagcaagt tacagacttt tatccgcatc attattccac tgacgatgcc
    18121 ggggattatt gccggatgtc tgctggtgat gctgccagcg atgggcctgt tctatgtgtc
    18181 cgacctgatg ggcggtgcga aaaacctgct gatcggtaac gtcatcaagg tacagttcct
    18241 caatattcgt gactggccgt ttggtgcagc caccagcatt acgctgacta tcgtaatggg
    18301 cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg aataagaagg tggaactcga
    18361 atgatcggtc gactgcttcg cggcggtttt atgaccgcta tctacgcgta cctgtatatc
    18421 ccaatcatta ttttgattgt gaactccttt aacagctcgc gctttggcat caactggcag
    18481 ggttttacca ccaaatggta tagcctgctg atgaacaacg atagcctgtt acaggcagcg
    18541 cagcattcac taacaatggc ggtgttttcg gcgacgtttg ctacgcttat cggttcactg
    18601 acggcagttg cgctgtaccg ttatcgcttt cgcggtaagc cgttcgttag cggaatgctg
    18661 tttgtggtaa tgatgtcacc agatatcgtg atggcaattt cgctgctggt gctgtttatg
    18721 ctgctgggta ttcagcttgg cttctggtcg ctgctgttct cgcatatcac cttctgcctg
    18781 ccatttgtgg tggtgacggt gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa
    18841 gcggcgaaag atctcggtgc cagcgaattt accattctgc gaaaaatcat tctgccactg
    18901 gcaatgccag cggtggcggc gggctgggtg ttaagcttta ccctgtcgat ggacgatgtg
    18961 gtagtttctt cattcgtcac cggaccaagt tatgaaattc tgccgttaaa aatttattcg
    19021 atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg caaccatatt actggtgctg
    19081 tcgctggtga tggttattgc cagccagctt attgctcgtg ataaaacgaa aggtaacaca
    19141 ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg
    19201 gcatgagcgc cgctcacgcc gatgacaaca acacgctgta tttctacaac tggaccgagt
    19261 acgtgccgcc aggactgctt gaacagttca ccaaagaaac cggtattaag gttatctatt
    19321 cgacttacga gtcgaacgaa accatgtacg ccaagctgaa aacgtacaaa gacggtgcct
    19381 atgacctggt ggttccttca acctattacg tcgataaaat gcgtaaagag gggatgatcc
    19441 agaagatcga caagtcgaag ttaacgaact tcagcaatct cgatccagac atgctcaaca
    19501 agccttttga cccgaataac gactactcca ttccgtatat ctggggtgcg acggcgattg
    19561 gtgttaacgg tgatgcagtg gatccgaaat ctgtcaccag ctgggccgat ctgtggaagc
    19621 cagagtacaa aggcagcctg ctgttgaccg acgatgcccg tgaagtgttc cagatggcgc
    19681 tgcgtaagct gggctactcc ggtaacacca ccgatccgaa agagattgaa gctgcatata
    19741 acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa ctccgataac ccggctaacc
    19801 cgtacatgga aggcgaagtt aacctcggca tgatctggaa cggttctgct ttcgttgcac
    19861 gccaggcggg tacgcctatt gacgtggtgt ggccgaaaga aggcggcata ttctggatgg
    19921 acagcctggc gatcccggca aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact
    19981 tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa
    20041 accttgcggc gcgtaagctg ttaagtccag aagtggcaaa cgataagacg ctctacccgg
    20101 atgctgaaac cattaaaaat ggcgaatggc agaatgacgt tggcgcagcc agcagcattt
    20161 atgaagagta ttatcagaag ctgaaagcag gacgttaatt caacaagcgg acggtggtat
    20221 taccgcccgt tttttagtta gcaaggatgt ggctaatgag tcaggacagt aaagttttat
    20281 cccgctcttt cgtcggtatt gtggtccgct tgtaggcctg ataagcgtag cgcatcaggc
    20341 aatgcttcct gctttataat ccctccagca acttttcaac aaattctggc accacctggc
    20401 ttgccgggcc gtaatatttc tcggcaaatt cattaccaac ctgactcggt tcaagattca
    20461 gttcaacggt gtgagcacca tgtagtttgg cttcgtgtac aaacccagca gccggataga
    20521 catgcccgga agtaccaatg gcaataaaga catccgccat cgacaacgcc atataaattt
    20581 catccatgcc gagtggcatt tcgccaaacc aaacgacgtg cggacgcaag ggtgccggga
    20641 agtggcaaca atggcattta tcttctggcg taacgtctcc ggtccagtcg agaacctgac
    20701 cactttgcga acaacgaact ttcagcaatt ccccatgcat atgaatgaca ttggtattac
    20761 ctgcgcgttc atgcaggttg tctatattct gcgttaccag caaaaagcga tcgccgagag
    20821 cgtcttgcag tttagccagc gcaagatgcg cggcgttagg ctgaatttct ggctgctgca
    20881 gctgtcgacg acgggcgtta taaaacgctt gcaccagttc aggatcgcga tcgaaacctt
    20941 ccggcgttgc cacatcttca acccgatgtt cttcccacag gccatctgcg gcgcgaaagg
    21001 tacgaatacc tgattccgca gaaattcctg cccctgtcag tacgagtact cttggttttt
    21061 ccattgcttc cggcaccact ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca
    21121 ggcgacgttt atttttacga aaacgactta accgatgacc ccgacgcgac agcatagcaa
    21181 cctctgttgt ttaatcggtt agatgtagga aggccgcacc acgcattcct cccgcatcac
    21241 cgtggcgcgc gcgttcaatg cgcggaacac gagctacagg taagagatga caaggcaacc
    21301 tgtccgccag ttgcgttgtg attgccggga aattcgataa gccaccacca atgacgacca
    21361 ggtcagggtc aacaatggtc aggatatttc ccagagaaac cgctaataaa tccagataac
    21421 gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata aagcgcaata atttcgggag
    21481 cctgcaacgg ttgatgataa tagtgttgat acagccacgc aaaaccgcga ccagacagat
    21541 aattttcaat gcagccatgc tgaccacagc cgcagcggcg taacgggaaa tccaacccca
    21601 tcatggttaa cgcatcaacc ggcagacgca tatggccaaa ctcgccggta atgtagcttt
    21661 taccagtaat cggtttgccg ttgaaaacca gcccgccgcc aacgccggtg ccgagaatta
    21721 accccatcac cagtggatat tgcgtaaatt catcctccca ggcttctgaa agggcaaaac
    21781 agttggcatc gttatcaagg cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca
    21841 gcggtttgcc gctggcagcg gggacattag cggcatacag cttaccatct tctgtttccg
    21901 gcattcccgg aataccgatg ccgacagaac ctttacagcc aaaacgttgg tcagcttcag
    21961 ctaccagctc acacactgca tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc
    22021 gcttttccca ctgcaactgc cgaccgctat caaacacgcc aagcgcaatt tttgttccac
    22081 caatatcaaa cccgtaatac attgccgctc cttgttttaa tgtactgcct ttactggccg
    22141 ctaaggactc gcgcagggtc aatattactg gcgcgccgcg ccggatacca acttgccaaa
    22201 agactcagca acaatgctgt gaccagtacg tagaagacgt ccagccagtg caattccgat
    22261 ggcaggaagt caataaaata gatatcgctg gagaggaact gatgaccgat cagcttttca
    22321 atccactcaa taatcggggt aagttgcagt gaaacaacta cgccgataac caccccacac
    22381 aggctgccga acagccctgc cagcaatcca taccagacaa agatggcgcg aattaaacca
    22441 tctttcgccc ccagcgttct taatactgcg atatcgccac tcttgtcttt caccgccatc
    22501 actaaggtgg agacgatgtt gaaacaggcc acgccaatca ccagtaccat cgccagatac
    22561 ataatggcgc ggatcatctg gatatcgcga tacatatagc cgtaagtacc aatccaactt
    22621 ttaatataaa catagctgtt ggtcacttca cccgcatcgc gtaccagctt attggcgttg
    22681 aaaacatccg tcattttaag ggcaatacct gacacgctgg aacccatatc aagatattgt
    22741 tgggcatccg ccagcgggat catggcaaaa ctgtgatcga gttgaccact caactgcaaa
    22801 ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca gtttatgctc gggattcgag
    22861 ttggggatca tgatcgacac ccaatcgccc tgcttcactt tcagcgcatc cgccacgcct
    22921 ttgccgatga taatttgctg ttcgcccgct ttaaaattgc gccacgcatc cccctgaaca
    22981 aacgagggta atgcgctcag acgctgttcc tgttgtgggt taacgccctt cacctggatt
    23041 gcgcgcagat tcgctccact ttccaccaga ccggtgaaat tgatatacgg cgcagcggcg
    23101 gcaatacccg gcactttctg cacgttatcc agtgcttcct gccagttagt ccatggctga
    23161 tttaccgctt cgatttcgcc atgcggcacc accgccagaa tgcggttatt cagttcgcgt
    23221 tcaaagccgt tcatcgcgct taagccgacg atcaataccg ccacgccgag ggcaatgcca
    23281 atggtagaaa tgacagagat tagcgacacc atgccgccgc gtcgccgtcc gcgactaaaa
    23341 cgcaggccaa tcaataacga taaaggcatc gccattactc cgcccccatc aggctcagtt
    23401 ccgccgtcag acgtccatca cgcatttcca gttggcggct catacgtttc gccagttgca
    23461 ggtcgtgagt gaccaccagg aaagcggtgc cctgcaagcg attcaactct ccaagcaact
    23521 ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt aggttcatcc gccagtacca
    23581 gacgcggatt attgaccagc gcacgggcaa tcgctacacg ctggcgttcg ccgccagaaa
    23641 gttcagatgg gcggtgattc gcacgatgct ccagccccac cgcttttaac atctccagtg
    23701 cacggctgtt gatttcagcg ggctttttct tgccaatcag cagtggcata gccacgtttt
    23761 ccagggcagt aaaatccggc agcaggtggt gaaactgata aataaagccc agcttctggt
    23821 tgcgcagttc tgctttcgcc gctgaagaca gtttgctcat cggctgaccg ttaaagatca
    23881 catcgccgga ggttggcgta tccagcccgc ctaacaggtg cagcaaggta cttttaccgg
    23941 aaccagagct accgacaatt gccatcattt caccttcgcc cacgctgaaa ctgacattgt
    24001 gcagcacatc ggtttgcaca ctgccttcct gatagcgttt gcacaggttg tcgcattgca
    24061 acaggatctt attcataacg taaagcctca gcgggttgtg tggcggcagc gcgccatgaa
    24121 gggtaaagcg tagacagcag cgcgatagcc atcgccacca gcgcaataac aatgacctgt
    24181 aaaggttcga tagccaccgg cagcgccgcg ccatcaagca ggacgccgat tatcggcatc
    24241 agattattta actggctggc aagcagtgcg ccaagcgccg ctccgaggat tgcaccgata
    24301 atcccggcgc tggccccttg caccataaag accatcatga tttgtcgcgg agttaagcct
    24361 tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca ccatcagccc cagcgaggta
    24421 ataatgttaa acgccgcaac agcgacaatc aggctcagca gtaagcccat catatttttt
    24481 tccatgcgta cggcctggaa cagctcgcct ttacggtcac gccagtcctg ccatttgctg
    24541 ccttcaggca gtttttgctg acttaaagag tcaactttca gcggctcatc cagccacaaa
    24601 cgccagccgg taatattggc tgccggataa cgcatcagac gcgaggcatc ctcaatattc
    24661 accagcattt catagccatc gacttcactg ttggcggcga aagtgccaat cacattgaac
    24721 aggcgctgac ttggaatacg ccccatcggc gtgaactggc tggcagatgg caccatcacg
    24781 cggatttgat caccgcgatt gacgcccaac tgtgaggcaa gttgttcgcc gaggatgaca
    24841 ttatatttcc ccggctcgag gtcagtttgt ttcacattga ccagatacgg cgttagtgga
    24901 tctttttgcg ccgggtcgat accgagcatc accccgaccg ccacgctgcg cgcgctttgc
    24961 agtaccacat caccggtagt aataggtgcg acgcgattaa cgccgtccag tttgactgcc
    25021 gtttccggga gttgctgcgg gttaagagag ccatgctcag aagagagaat tgcctgtggc
    25081 atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc cgttcatcac tgacaatact
    25141 gtgaccagcg ccatcacccc gagggtaatg ccgatggtag aaagccagga gacgaaacga
    25201 ccgaagcgat ccgctgcacg cccacgcatg taacgcaagc caataaatag agcgacaggt
    25261 tggtacatga aatccgtctg gttgctgtag caaagacacg agtatataag cgaaagcaag
    25321 atgggtaaac gtctgaactg taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat
    25381 caatcggcta aattgccatg ttttatcatc aatcttgccg cctgatctta atctgaaaaa
    25441 tcatccaata cagacagata cccattacac ctcgggtcat aaccagcagg atcaaccgca
    25501 tgtaaattaa aaaagagcgt gttgttctga tgaaacaaaa agagctatgg attaaccaga
    25561 tcaaagggtt atgtatttgt ctggtggtga tttatcactc ggtcattacc ttttatccgc
    25621 atatgaccac ttttcagcat ccgttatcgg aagtcctgag caaatgctgg atctatttca
    25681 atctttacct tgcccccttt cgtatgccgg tttttttctt tatatccggc tatttgattc
    25741 gccgctatat cgacagcgta ccgtggggaa attgtctcga taaacggatc tggaacatct
    25801 tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc acctgagcgc gatttaagca
    25861 atgcctccaa tgccgcttat gccgattcca ccggtgagtt cctgcacggg atgatcaccg
    25921 ccagcaccag cttgtggtat ctgtatgcgt taattgtcta tttcgtggta tgtaaaattt
    25981 ttagccgtct ggcgctgcca ctattcgcct tgtttgtact gctgagtgtg gcggttaatt
    26041 tcgttcccac gccgtggtgg ggaatgaaca gtgtgatacg caatttgcct tattacagcc
    26101 ttggcgcatg gtttggcgca acaataatga cctgtgttaa agaggtgccg ttgcgccgcc
    26161 atctgctgat ggcttctttg cttaccgttc tggcggtcgg tgcctggttg tttactatct
    26221 cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa gctgttttat cagtacgagc
    26281 aacgattcgg tatgcgctcc accagcctgc tgaatgtgat tggctccaac accattgcta
    26341 tctacaccac ccatcgcatt ctggttgaaa tattcagctt aactctgctt gcgcaaatga
    26401 acgcagcacg ctggtcgccg caagtcgaac tgacactcct gctggtttat ccctttgtca
    26461 gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa actttcacag cgtgcattca
    26521 gcgatctgtt gttctccccg ccttcactgc ccgctgccgt cagttactct cgttaaacca
    26581 tcaccccagc cggggcgatt caatttgcta aaccatgtcg attacggata atgaacagca
    26641 ttgcttatca gagatatgcc cccatatgtt gaggcatatc ctaacgagaa tctgacaacc
    26701 gttatgcctg aacaatatcg ttatacgctg cccgtcaaag cgggtgagca gcgtctgctg
    26761 ggcgagttaa ccggcgcagc ctgtgcaacg ctggtagcgg aaattgccga acgtcacgcc
    26821 ggtccggtgg tactcattgc accagatatg caaaatgctc tgcgtttgca tgatgaaatc
    26881 agccagttca ccgatcagat ggtgatgaat ctggcggact gggaaactct tccctacgac
    26941 agtttttcgc ctcatcagga cattatctcc tcgcgccttt ccacccttta ccagctaccg
    27001 acgatgcagc gtggcgtact gattgttccg gtgaatacgc ttatgcagcg cgtttgccca
    27061 cacagttttc tccacggtca tgcgctggtg atgaaaaaag gtcagcgcct gtcacgagat
    27121 gcattacgaa cccaactgga cagcgccggt tatcgctatg ttgaccaggt gatggagcac
    27181 ggcgaatacg ccacgcgcgg cgcgttgctg gatctcttcc cgatggggag tgagctgcct
    27241 tatcgtcttg atttctttga tgatgaaatc gacagcctgc gggtgtttga cgtcgacagc
    27301 cagcgcacgc tggaggaagt agaagcgatc aatctgctgc ccgcacacga atttccgacc
    27361 gataaagcgg caattgaact gttccgcagc cagtggcgcg ataccttcga agtgaagcgc
    27421 gatccggaac atatttacca gcaagtgagt aaaggcacat tacctgccgg gatcgagtac
    27481 tggcagccat tgttcttcag cgaaccactg ccgccgctgt tcagttattt ccctgccaat
    27541 accttgctcg tgaatactgg cgatctggaa accagtgccg aacgtttcca ggctgacacg
    27601 ctggcgcgtt ttgagaatcg cggcgtcgat ccgatgcgcc cgctgttgcc accacaatcg
    27661 ctctggctgc gggtggacga gctcttctca gagctgaaaa actggccccg agtgcagcta
    27721 aaaactgaac atttaccgac aaaagccgcg aatgccaatt taggtttcca gaaactgcca
    27781 gacctggccg ttcaggcgca acaaaaagcg ccgctggatg cgctgcgtaa gttcctcgag
    27841 actttcgacg gtccggtggt gttctcggta gaaagtgaag gtcgccgtga agcgctgggt
    27901 gaactgctcg cacgaattaa aattgctccg caacgcatta tgcgtcttga tgaagccagc
    27961 gaccgtgggc gttatctgat gattggcgct gccgaacatg gttttgtcga taaggtgcgt
    28021 aatctggcgc tgatctgcga aagcgatctg ctcggtgaac gtgttgcccg ccgtcgtcag
    28081 gattctcgcc gcaccatcaa ccccgataca ctgatccgta accttgcgga gctgcatatt
    28141 ggtcagccgg tggtccatct ggagcacggc gtcggtcgct atgccggaat gaccacgctg
    28201 gaagctggcg gcattactgg cgagtacctg atgctcacct atgccaacga cgccaaactg
    28261 tatgttccgg tgtcgtcact gcatctgatt agccgttacg caggtggcgc ggaagaaaac
    28321 gccccgctgc ataaacttgg cggcgatgcg tggtcacgcg cgcggcagaa agcggcggaa
    28381 aaagtgcgtg atgtggcggc ggaattgctg gatatctacg cgcaacgcgc cgccaaagag
    28441 ggcttcgcgt ttaaacacga tcgtgagcag tatcagttgt tctgcgacag cttcccgttt
    28501 gaaaccacgc cggatcaggc gcaggccatt aatgcggtac ttaacgacat gtgtcagccg
    28561 ctggcaatgg atcgtctggt gtgcggcgat gttggctttg gtaaaacaga agtggcgatg
    28621 cgcgccgctt tcctggcagt agataaccac aagcaggtag cggtgttggt gcctaccacc
    28681 cttcttgcgc agcagcatta cgacaacttc cgcgaccgtt tcgccaactg gccggtacgt
    28741 atcgaaatga tctcccgttt ccgcagcgcc aaagagcaga cgcaaatcct tgcggaagtg
    28801 gcggaaggga aaatcgatat tctgatcggt acgcacaaac tgctgcaaag tgacgtcaag
    28861 tttaaagatt taggcctgct gattgtcgat gaagaacacc gcttcggggt gcgtcataaa
    28921 gagcgcatta aagcgatgcg cgcgaacgtg gatattctga cgcttactgc aacgccgatc
    28981 ccacgtacgc tgaatatggc aatgagcgga atgcgtgacc tgtcgattat cgccacgccg
    29041 cccgcccgtc gtctggcagt taaaaccttt gtccgtgagt atgacagcct ggtggtccgg
    29101 gaggcgatcc tgcgtgaaat tttgcgcgga ggacaggttt attatctcta caatgatgtg
    29161 gaaaacattc agaaagccgc cgaacggctg gcagaactgg tgcctgaagc acggattgcc
    29221 atcggtcacg ggcagatgcg cgagcgcgaa ctggaacggg tgatgaatga tttccatcat
    29281 caacgtttca acgtgctggt ttgtaccacc attatcgaaa ccgggatcga catcccgaca
    29341 gccaacacca ttatcattga acgtgcggat cacttcggtc tggcgcagct gcaccagtta
    29401 cgcggtcgcg tcggacgttc acatcatcag gcatatgcat ggctgctgac gccgcatcca
    29461 aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga ttgcctcgct ggaagatctc
    29521 ggggcaggtt ttgcgctggc aacgcacgat ctggagattc gcggcgcggg tgaactgctt
    29581 ggcgaagaac aaagcggctc aatggaaacc atcggtttct cgctgtatat ggagttgctg
    29641 gaaaacgccg tcgatgcact gaaagccgga cgcgagccgt cgctggaaga tctcaccagc
    29701 cagcaaacag aagtcgagct gcggatgccg tcgctattgc cagatgattt catccctgac
    29761 gtgaacacgc gtctgtcgtt ctacaaacgt attgccagcg ccaaaacgga aaacgaactg
    29821 gaagagatca aagtcgagct tatcgatcgc ttcggcctgc tgccagatcc ggcgcgtacc
    29881 ctgctggata ttgcccgtct gcgccagcaa gcgcagaaac tggggatcag gaagctggaa
    29941 ggtaatgaga aaggcgggat gatcgaattt gccgagaaga atcacgttaa tccggcctgg
    30001 ttgattggtt tgctgcaaaa acagccgcag cattaccgcc ttgatggtcc gacgcgcctg
    30061 aaatttattc aggatttgag tgagcggaaa acgcgtatcg aatgggtacg ccagtttatg
    30121 cgtgaactgg aagagaacgc gatcgcttaa tgtaaatcgg gggcgtttct gcgcccccat
    30181 ctgcaacatt tacaaattct ttgcactttc ctgcactatc cgacactgtc accatccata
    30241 attcaggctt atctgtttat tacaataacc ttatatttat tatggatttt tggtgatgat
    30301 caaaacgcgt ttttctcgct ggctaacgtt ttttacgttc gccgctgccg tggcgctggc
    30361 gctaccggca aaagccaaca cctggccgct gccgccagcg agcagtcgtc tggttggcga
    30421 aaacaaattt catgtggtgg aaaatgacgg tggttctctg gaagccatcg ccaaaaaata
    30481 taacgtcggc tttctcgctc tgttacaggc taaccccggc gttgatcctt acgtaccgcg
    30541 cgcgggcagc gtgttaacga tcccgttgcc aaccctactt ccagacgcgc cgcgcgaagg
    30601 cattgtgatc aacattgcgg agctgcgtct ctattactac ccgccgggta aaaattcggt
    30661 aaccgtgtat ccaataggta ttggtcagtt aggtggtgac acgctgacac cgacaatggt
    30721 gaccaccgtt tcagacaaac gtgcaaaccc aacctggacg ccaacggcaa acatccgcgc
    30781 ccgttataaa gcacagggaa ttgagttgcc tgcggtagtg ccggctggac cggataaccc
    30841 aatgggccat catgcgattc gtctggcggc ctatggcggc gtttatttgc ttcacggtac
    30901 gaacgccgat ttcggcattg gcatgcgggt aagttctggc tgtattcgtc tgcgggatga
    30961 cgatatcaaa acactcttta gctaggtcac cccaggcacc aaagtgaata tcatcaacac
    31021 tccgataaaa gtctctgccg aaccaaacgg tgcgcgtctg gttgaagtac atcagccgct
    31081 gtcagagaag attgatgacg atccgcagct gctgccaatt acgctgaata gcgcaatgca
    31141 atcatttaaa gatgcagcac aaactgacgc tgaagtgatg caacatgtga tggatgtccg
    31201 ttccgggatg ccggtggatg tccgccgtca tcaagtgagc ccacaaactc tgtaaatcct
    31261 ggataaaaaa aatccccgcg acacggcatg tcgcggggaa aaaatcagca gtagcaggca
    31321 ttaatgaggg ttaatgctta tttataaatt actgctgttc catggagggt attcggaccg
    31381 gttacagaag taatacggaa agattttgcg cccatctcat ccgctttttg cgccagctgc
    31441 tcttccagcg atcccagatt tgtccccgcg ttagcactga tagtaccgac tttttgttgg
    31501 ccttctggcg ttgactgaac ttcgacagcc gcaaagttgg caaatgacat ggagcttaaa
    31561 atcgccgcag cgatgagggt ttttacgttt ttcataatag tggccttatg cagatgaatg
    31621 acgtcgcaag aaattactta acgatcgata actaaatgat agatgtgatc tggatcacat
    31681 acaagatatt ttttataaca atcattaatt aatttaaaaa tctttaaatt tcagtcaaat
    31741 agaaagcaat tatttttatg ggatctggac tggtgaacgg aggtgattat ttttataata
    31801 acctttcatt cacagtcacc aggtacaacg gcatcatggc aactgactca acacaatgtg
    31861 taaaaaaaag ccgtggccgc ccaaaagtgt tcgacaggga tgccgcgctt gataaggcca
    31921 tgaaattgtt ctggcaacac ggttatgaag cgacttctct tgcggacctc gtcgaagcga
    31981 ccggagccaa agcgcccacg ctatacgcgg aatttaccaa caaagagggg ttatttcgtg
    32041 ccgttctcga ccgctatatc gatcgttttg ccgctaagca tgaagcacag ctgttttgtg
    32101 aagagaaaag cgtggagtct gcgctggctg actattttgc tgccatcgcc aactgcttta
    32161 ccagcaaaga caccccggca ggctgcttca tgatcaacaa ctgcaccacc ctctccccag
    32221 attcaggaga tatcgccaat acgttgaaat cacgccatgc gatgcaagag cgcactttgc
    32281 agcagttttt atgtcaacga caagcgcgcg gggaaatccc gacccactgt gacgtgacac
    32341 atctggcaga attccttaat tgtattattc aggggatgtc gatcagcgca cgcgaaggtg
    32401 catcgctgga aaaactgatg cagattgccg gaacgacttt gcgtttatgg cccgaactgg
    32461 tgaaataagt cgcaaaaaaa gcccctcagc ctgagcgaat gaggggccaa attgcagagt
    32521 acagccttgt tatactttgt tattcaaaat cagttgcccg ttactatcta gcgggatctg
    32581 cgtacccgga tcgcggtcca tgcggatttt gccctgctga tcgccaatct tataggtcac
    32641 atcataaccg agcatttttt ctgacttgtc atacaccgtt ttacaacgct gttgcgtagt
    32701 cgtgtaagta tcgctttcct ggagagagcc ctggatctgg ttaccggcat atccaccacc
    32761 cagcgccccc acaacagtgg cgacatcttt accgcgacca ccaccaaact gatgccctat
    32821 cacgccacca gcaacagcgc cgagcaccga cccggtaatg cgattttcat cctgcaccgg
    32881 tcgacgatgg gtcactgtga cgttgcgaca ctcctgacgc ggtgttttaa ccgtttcctt
    32941 gattggggtt gcagaaacaa cctgagcgta ttgcgggccc cgttcaaaca cgttcagact
    33001 ggccactgcc gctacgccca acgcagctgc gacaccaatc ccgatacccg ccaacattga
    33061 tttattcacg ggaacacctc cttctttgca gttatgccaa tcatgcaagc gaaggagaaa
    33121 agcagctatc agacagaaga tgaaaagcgc gggaatgact ggaaatactt aaggattcgg
    33181 aggagtctga aggaatgcct gatgcgacgc ttaacgcgtc ttatcaggcc taccgctcgt
    33241 accatttgtc cagggcggat aaggcgttta tgccacatca gccagtgtac gtcgattaat
    33301 gcaacttcaa acgcggacgg ataacgcggt taatactccc caccagcatc attaatccgg
    33361 ttttaaagta accatgcagc gcaatctgat gcattcggta tagcgagata tatacaaagc
    33421 gcgcaattcg tccttcaatc atcattgagc cgcgcgtcag gttacccatc aggctaccaa
    33481 cggtggagaa gttcgacagc gataccagcg aaccatgatc tttatactga taatttttca
    33541 gcggcttacc gttcatctgc gccagaatgt tgttcattgc gcaagtcgcc atctggtgtg
    33601 cagcctgagc acgcggcgga acaaagcccc cttccggacg cgggcatgac gcgcagtcgc
    33661 caatagcgta aatgtctgga tcgcgagtgg tttgcagcgt cggttccacc accagctggt
    33721 tgatacggtt agtttcaaga ccaccgatat ctttcaggaa gtctggcgct ttgatcccgg
    33781 ctgcccatac catcagatca gcctcaatat attcgccatc tttagtgtgc aggccgcctt
    33841 catcagcact ggtgaccatg gtttgcgtca gcacgcgaac gccaagtttc gttagctcgt
    33901 tgtgggccgc agcagagata cgtggcggta atgcaggcaa aatacgttct cccgcttcta
    33961 ccagcgttac gttcagggct tcgttggtca ggcctttgta accgtagctg tgcagttgct
    34021 tgatcgcgtt gtgcaattca gcggagagtt ctacacccgt cgcgccgccg ccgacaatcg
    34081 caatgttcac tttgccattt gcgcccaggt tggcagagta tttcaggaac agattcagca
    34141 tctcctggtg gaagcgacgc gcctggtgcg ggttatcgag gaaaatgcag ttctctttga
    34201 cgcctggcgt attgaaatcg ttagaggtgc tacccagcgc catgaccagg gtgtcatagg
    34261 cgattttacg ttccggaacc aacagttcac ctttctcgtc gcgtagttct gcaatagtga
    34321 ttgttttcgc ttcacgatca atatcaatga cggaacccaa ctggaactgg aaaccatgat
    34381 tgcgcgcatg ggccagatag ctcaacgcat cgacgccttc atcaagcgag ccagtcgcca
    34441 cttcgtgcag cagtggtttc cacaggtggc tgtggttacg atcgaccagc gtaattttgg
    34501 cttttttctt acgtcccagc ttatgcccca gctgtgttgc catttccagc ccaccagcac
    34561 cgccgccgac aatcacaatc tttttcaatg gcgtagtcaa cgtgaccccc ttaaatttat
    34621 taaccaattg ttaattaatg gttattaaca tagctcttaa ttaacaacag gttacagcaa
    34681 tgaaaatgtt cttcgctatt gagaataaca tgaatggtgc attggtcata ccaaaattga
    34741 tgttaatcaa gttttgttgc ttaaaagata ggcaacttta ccgggggaga tcaaaaaata
    34801 gcgtttatta tttgagcaaa ttcggaagag taatataggg tgaagcgtat atcgagggcg
    34861 tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg actgtcggat
    34921 gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac cggcaaggtt
    34981 acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg ctgtaaatgc
    35041 ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc gtaataatga
    35101 tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga caaaataacc
    35161 agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc aatatcggca
    35221 tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa attagggttg
    35281 aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta tccgcccagg
    35341 ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt gtccacttct
    35401 ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat caagcgtaca
    35461 tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact gttagagtca
    35521 aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt gacctgcttt
    35581 ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgatcgctt tccagcgagc
    35641 cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg taacacgttc
    35701 tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat tattgcagac
    35761 caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat aactgcccat
    35821 aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa aacccagttc
    35881 ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg gatcaacatc
    35941 actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga ttaaggaact
    36001 gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc
    36061 tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat
    36121 tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt tttgtggatc
    36181 ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca gcactggcgc
    36241 aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac ccgcctcttg
    36301 cgccagtttg ccgccctctt ccattcccaa cagcgcagca aaggattgtg ctgccggtaa
    36361 gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct tcctgatcta ccgccaccac
    36421 cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact gggcaggatc
    36481 atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca gtatttcacg
    36541 ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca cactgctctc
    36601 cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata aattgttgat
    36661 cgccggtttg tcgccagcgg tactcaaacc accctccttt gagcatcagc agccagggaa
    36721 accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga gtggcatagt
    36781 cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc agttccagcg
    36841 cgatatcacc atctccagca tactcccagt cgatgagttt taaccctgat gcgctatgca
    36901 ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggt cgtggttccc
    36961 gcgctttgcg cagacgtttt aacattcgca gccaacccac agtccgccgc gccggatcgc
    37021 tttgctgcca gtacagttcc agtaagggca acagcgttat tcgccagcca aaacgtggtt
    37081 gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa tacgttttta
    37141 ccgcgccggg cagatagtcg actaccatcc agtcacggag atataaatgc ggcttcggtg
    37201 caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg gactgcggcg
    37261 catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca atgagaaaac
    37321 taccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg
    37381 acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt acctgaccag
    37441 ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc gttaacgttg
    37501 ccagaggcgc tggagtacag cacgtaatga gcgccgacat tgcgggcaat gcctatggct
    37561 ttactacggg tgcctaaact gtcctgcggc gacaaaccta actgttgctt agccatcgac
    37621 agttgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt
    37681 tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct atcaaccagc
    37741 aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat cggctgcatt
    37801 gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgccgttcg atcttcgtgc
    37861 tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt
    37921 tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc aggttcacgt
    37981 tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt
    38041 tttgtcatca agattcaccc ctttacaaat atagataaag tctgactttg tgcgccccca
    38101 gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc gggaatggta acgctgcgtg
    38161 gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa cgataatgaa
    38221 cggtaacggg ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt tgaatatcag
    38281 acgtcgaaag gacgggcttt tctgcactga tgcccgcagc caataaagat gactccatca
    38341 ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc accagcagta
    38401 acaacaccag agacaccagc ccaaagcatc cttttctcat cgttacagac ctttatgcgc
    38461 cagcattggt cccagcggac ggccacccaa caagtgcata tggatgtggt aaacctcttg
    38521 tccgccatgg cggttggtat tcatgatcag acgatagcca tcttcggcaa taccttcttg
    38581 ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat gctcagctga
    38641 cacgtcgttc acagtcggaa tgaggatatt cggaatgatc agaatatgcg ttggcgcttg
    38701 cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat cggaggggat
    38761 ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt cctttttcaa
    38821 atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag tatggtcgag
    38881 ttaaacctgc tctttcaacc ttaactcacg atttcttaag caaaaaatcg ttttgctgcc
    38941 gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac attctctgtt
    39001 tggtttacac gacaaatatg aatgcgtata tttctcattt gcatttgcaa gcaaaattat
    39061 tcgcacacta aatataaccg ctcatcaccc tcctccggca tgagcctgac gacaacataa
    39121 accaagagat ttcaaatgct ttcaacacaa tttaacaggg ataatcaata tcaagccatc
    39181 accaaaccgt cactacttgc cggttgcata gcactggcac tattaccttc tgccgctttt
    39241 gctgcaccag ccactgaaga aacggtgatt gttgagggtt cagccacagc tccagatgat
    39301 ggcgaaaatg attacagcgt aacgtctacc tctgcgggta ccaaaatgca gatgactcaa
    39361 cgtgatattc ctcagtcggt cactattgtt agccagcagc ggatggaaga tcagcagtta
    39421 caaacgctgg gcgaagtgat ggaaaacacg ctggggatca gcaaaagtca ggcggattcc
    39481 gatcgtgctc tttattattc ccgcggattc cagatcgata actatatggt tgatggtatc
    39541 cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac tttctgatat ggcactgttt
    39601 gaacgcgtag aagtagtgcg tggcgcgaca ggactcatga ccgggacggg taatccatct
    39661 gcggcaatta atatggttcg aaaacacgcg accagtcgtg aatttaaagg cgatgtctcg
    39721 gcggaatacg gtagctggaa caaagaacgg tatgtggcgg atttacaaag cccactcacc
    39781 gaagacggta aaatccgcgc gcgaattgtc ggcggctacc agaataacga ctcatggctg
    39841 gaccgctaca atagtgaaaa gaccttcttc tcgggcattg tcgatgctga tttaggcgat
    39901 cttacgacgc tttcagccgg ttacgaatat cagcgcattg atgttaatag cccaacctag
    39961 ggcggtttac cgcgctggaa tactgatggc agcagcaaca gttacgatcg cgcacgcagt
    40021 accgcacctg actgggcgta caacgataaa gagatcaaca aggtctttat gaccctgaag
    40081 cagcggtttg ctgatacctg gcaagcgaca ctgaatgcca cccactctga agtcgaattt
    40141 gacagcaaaa tgatgtatgt cgatgcctat gtaaacaaag cggatggtat gctggttggg
    40201 ccatacagta attatggacc tggctttgat catgtcggcg gcaccggttg gaacagtggc
    40261 aaacgtaaag ttgatgcgct ggatttgttc gctgacggta gttatgaatt gtttggtcgt
    40321 cagcacaatc taatgtttgg tggcagttac agcaaacaaa acaatcgtta cttcagttca
    40381 tgggccaaca tcttcccgga tgaaattggc agtttctaca actttaatgg caatttccca
    40441 caaaccgact ggtcaccaca gagcctggcg caggacgata ccacacatat gaaatcgtta
    40501 tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc tgatcctcgg cgcacgttat
    40561 accaactggc gggttgatac gctgacttac agcatggaga aaaaccacac cacgccttac
    40621 gctggtctgg tgtttgacat caatgacaac tggtcgacct acgccagcta tacctctatt
    40681 ttccagccgc aaaatgatcg tgacagttca ggcaaatatc tggctccaat caccggtaac
    40741 aactacgagc tgggtctgaa atcggactgg atgaatagcc gtctgaccac cacgttagcc
    40801 atcttccgta ttgagcagga taatgtcgct cagtccaccg gtacacctat ccccggcagc
    40861 aacggcgaaa ccgcctataa agcggtggat gggacagtca gtaaaggggt ggaatttgaa
    40921 ctcaacggcg caattaccga caactggcag ctgacatttg gcgcaacgcg ctatcttgca
    40981 gaggataacg aaggaaacgc cgttaatcct aatctgccac gcaccacggt taaaatgttc
    41041 accagctatc ggttgcctgt catgccagag ttgacagtcg gcggtggtgt taactggcaa
    41101 aatcgcgtgt ataccgacac cgtgacaccg tatggcacct tccgcgccga gcaaggtagc
    41161 tacgcgctgg tggatctctt cacccgctac caggtgacga aaaacttctc gttacagggg
    41221 aacgtcaata acctgttcga caaaacctac gataccaaca tggaaggttc tatcgtctac
    41281 ggcgcaccgc gtaatttcag cattaccggc acgtatcaat tctgaataac acctgtaaaa
    41341 aaggcagcca tctggctgcc ttagtctccc caacgtctta cggattagtg gttacggatg
    41401 tactcatcca tctcggtttt caggttatcg gatttagtac cgaaaatcgc ctgaacacca
    41461 gaaccagcaa ccactacgcc cgctgcgccc agtttcttca ggccagcctg atccacttta
    41521 gacacatcag caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt
    41581 tctttaccac caaatgcagc aaccagagcc ggtgccattt cgctggtacc tgttgctttt
    41641 gcatcttcag tcgcgtcttc acgacccggc gttttcagat ccagtgcttt aatcagcacg
    41701 cggaagatgg tgtagtaaac aatcgcataa ccgataccga cgatcgggaa cagccacagt
    41761 ttgctgctgt taccagacag aacgatgaag tcgatcagac cgtgcgagaa cgacgtaccg
    41821 tcacgcattc ccagaagaat acagattggg aatgccaggc ctgccagaat cgcgtggatg
    41881 atgtacagga tcggcgcaac gaacatgaag aagaactcga tcggctcggt gataccggtc
    41941 aggaacgagg tcagcgccgc ggagatcata ataccgccca ctttcgcgcg gttttctggt
    42001 ttagcagagt gccagatagc aattgcggca gctggcagac cgtacatttt gaacaggaag
    42061 ccaccagaca gtttacccgc agtcgggtca cccgccatat aacgcggaat gtcgccgtgg
    42121 aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg tacgttccag
    42181 atgtggtgca gaccaaacgg taccagacaa cgttcgatga aaccgtaaat gccaaacgca
    42241 actaccgggt tctggtaagc agcccactga gagaaggtct ggattgcaga accaatcggc
    42301 ggccaaatga aggacagcac aacgccagta aagatggcag ccaggccaga aatgatcggc
    42361 acaaagcgtt taccggcaaa gaagccaaga tactcaggca gcttaatacg gtagaaacgg
    42421 ttaaacatgt acgctgcaat cgcaccggag ataatccctc cgagtacgcc agtatccgcc
    42481 aagtgtttag aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc
    42541 atggttttaa ccatgatgcc ataggcaaca actgcggcca gcgcggatac gccatcgtta
    42601 ttggtaaagc cgagggcgac accgatcgca aaaatcagtg gcatgtttgc aaagacggaa
    42661 ccgcctgctt ctgccataac atgcgataca acggcgggca gccagctgaa attcgcggaa
    42721 ccgacgccca gcagaatacc tgcgataggt agtacggata ccggcagcat cagcgattta
    42781 ccgacctttt gcaggttagc aaatgcattc ttaaacataa ttgagagtgc tcctgagtat
    42841 aggtgctttt tttacgttct cacgcgtggc aaggggggag agcctcgccg tgtacagggc
    42901 atctaagcgc cctttattta ttacacagag taaaataatt cagtgccaat ttgtttgacg
    42961 gctatcacgt ttcaacttca gatcattgga gaagttgcac attttgacaa aacatatgtc
    43021 acaatatttc aaacgcggtt attcaccgcc caattttcgg cggtgaactt tactcgtttt
    43081 agccattaat tacgagcttt aaaaaaactc attcaacgga tggattgaag gcgggaagcg
    43141 tcgatgtgaa acagacgggc gaagttatcg gtggttacct gcgccagttc ttcaacggca
    43201 acacctttca acacagccat gtattctgca acgtcacgaa ccatcgcagg ttgattctct
    43261 tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag tttccaccag taatcgatcc
    43321 agggggacat aacgcgcagc atcgcacagt tgctccgcat tacggaaggt cacaatgccg
    43381 gaaaaggaga tgtaaaatcc gagatccagt aatttacccg ccgtttctct gtcctctgta
    43441 aaacagtgta gtacgccacc gcaatccgtc actttttctt cgcgcagaat tgccagcgta
    43501 tcggcacggg cgtcacgggt atggacgata accggcttgt tcagttcacg accaatctgg
    43561 atatgatgga tgaaggactc ttgctgacgt actttagttt ccggtgtgta ataataatcc
    43621 agcccggttt cacccagcgc tacaacaccc tcttctgccg ccagacggcg taaatcttct
    43681 acatcgtagg gatcattctg gttgagtgga tgcacgccac aagaaaatac gacgttgtca
    43741 cgttcgccta ccagatcccg catatgtaaa taacccggta atgttgtggc gactgccaga
    43801 caaaatttca catcgcgtgc ggcggctttc gccagaacgt catccacgtc cttatgcaaa
    43861 gattcataat ccagaccatc gagatggcag tgtgagtcga ctaaaaacat gatgtctctc
    43921 ttaaagatga ggaaccggta gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa
    43981 aagatcggtg atgagaagct cgcggttgat gcctgtgaca gacattaact gttcacgaat
    44041 gtggcaaaca tcccccagta tagcctgcag gcgcgaggga gaaagatggt ttgccagttc
    44101 ggcgaccagg cccggcacat caacattggt caccagcgca gcgccatgat ggcgttttag
    44161 cgcatccatc agcaacgttg ccacccagtg taaacgcgcc ggagcttgtt catgattaag
    44221 ggccgccagc agcgaatacc agtcgcccga tggcacgcta tatgccaacg cctgacacaa
    44281 tgtttcacga gcctgccagt tatctccctg aaacaacgcc agtgccgcgc caggcgaacc
    44341 ggcgcttaag cgcaatgcgg caagtaatgc atcctgtgac attgtcactt cgcgtgaaag
    44401 ccaagtcacg gcgtactgtt ctggaggcgg cgcaaggtaa tgtaaccgac aacgactacg
    44461 taatgttgcc agtaaacgtt caggctcgcg ggtagccagg aaaaaccagg tttctgctgg
    44521 tggctcttca agcgttttca gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc
    44581 ggttacccag acaactttcg caccacctag gcgtgcgtgc tcattcagct tttcggtgac
    44641 ctcacgtacc gcatcaatgc ccagcgtatt tttccctttt tcgggagcca gggtgtagta
    44701 atcgggatgc gtgccagcct gcatcaactg acatccacga cagtgaccgc aacttttgtg
    44761 gccctgcggt tgttggcaga gtaaataacg gctcagggcg tagattaaag catcatcgcc
    44821 catgcccggt aacgcctgaa tgagtagcgc atggtgacct cttccggcct gatagctggc
    44881 taccagtttt tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc caactccttc
    44941 acccagtggg tcacggtagt gcggattgca tccatcacgg cctccagcgg ctgggtggca
    45001 tcaatggtat gaatactttt atcttgtgca gccagttcca gatagcgggc gcgggtgcga
    45061 ttaaagaaat cgaaagactc ttgctcaata cgatccagct cgccgcgcgc acgcgcgcgt
    45121 tttaagccaa cttccggggt aacatcgaga tagagcgtta agtcggggcg aaaatccccg
    45181 agaacagcat cacgcagtgt tgccagcata tgttggtcaa taccacgtcc tccgccctga
    45241 tacgcctgag tggagagatc gtggcgatcg ccaatcaccc aggtgccgtt agccagcgca
    45301 ggtttgatga ccgtttctac cagttgaacg cgcgcggcat aaaacatcag aacttcggct
    45361 ttatcggtaa tgacttcatc gcctaccgat ttgatatcca gcaccaggct tcttaacttt
    45421 tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat acccagttgc
    45481 tcgagcgtct caaccaccac attacgcgcg gtagttttgc ctgcgccttc cagcccctca
    45541 atgacgatat acttactgcg catttttttc cttaagcact ttcagataat cctgcacaga
    45601 cttgttatga ctggcaagat tggtattaaa cgtgtgacca cctttaccat cggccacaaa
    45661 atagagatac ggcgtttttg ccggatgcgc agcagccttc agcgaatccg cccccggcgt
    45721 agctatcgca cccggcggca aaccggtaat ggtataggtg ttatacgctg tcggcgtttc
    45781 caggtctgca cgagaaagtt tgccattata acgctctccc atcccgtaaa tcacggttgg
    45841 gtcggtctgc aggcgcatac caatgcgtaa acggttgata aataccgagg caacctgatc
    45901 gcgttcactg gcaacggcgg tttctttttc gataattgat gccatcgtca ccagctgatt
    45961 tttatcttta taaggcagac cgtccgcacg cccttcccag gcgctatcga ccgctttcac
    46021 cattttcttg tgagctcgct tgagtaacgc gacatcggtg gtattggcgg tatacatcaa
    46081 ggtgtctggc cagaaccaac cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc
    46141 tacggtggcg tacttatcgt cactcagcgt gtgcttgata tacggagcct cacgcaattg
    46201 cttgaggtag tcgctcagac gcatcccttc taccagtcgc agcgggaact gtgcttcttt
    46261 accgctttcc aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt
    46321 cccggcttta aagtgagaaa gatccggttc gatacgcagc agccattgaa aaacccgtgg
    46381 gcgattgatg atcttatcag cataaagctg ttcaccgagc gccagacgtc cggtccctgg
    46441 cttcagggta aatatcgtct cttctttgat aagcaatttg ctgtcggcaa gatggcgaac
    46501 cttccagacg cccacaccag cggcgatacc cagtaccacc agcaataaca agataatcaa
    46561 taacactttt ttcatgacta attcgggcgc tcacaaagtg gggctaaata ttcatataac
    46621 gttgctgacg aaaaggtgac atcgccacag gcacgtacgg gcatcactgg cattaacgca
    46681 ttacaaataa ccatctcatc tgcctgcaac gcctcctcca gcgaggcttg cacttcgaca
    46741 agctgataag aggattgtgc cagtaaacgg atacagaatt gtcgcataat gccgttaaca
    46801 cctgcctgat ccagtcgcgg cgtataaact acattgccct tccgccagaa caaattagcc
    46861 gcacagcatt ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc
    46921 tgctcaagat gagagcgaat caatacttgc tcaagccgat taagatgttt aatacctgca
    46981 agatgaggat tgcgccccag ccgcaccggg cttagcgcca acgtcatccc ttcgttacgc
    47041 aaacggtcgt aatgtgcagg ataagccgta acggagagaa tccgcgttgc tggtccgctg
    47101 ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga tcacgacttt cagcacacca
    47161 ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa gctgaggcca gaagtcacag
    47221 gaaatcatca accgctgaca agcatcctgt agtcgctgga tatgcgccga taacaaactg
    47281 actttaccgt cgataactct ggcggtggta aaacaaccat caccaaactg cgttgcccga
    47341 tcgcttactg ccagcgattc ctgcttataa ccgttaatta agaacatacc ggctccttat
    47401 cagagaattg ttagtgtggc agcatgttca ctacggaaca agtcggaata aaagctaaga
    47461 aaaaaggccc gcaagcggac cttttatatg ggtggaaaat gacaacttag atctttttaa
    47521 agatcaaaga accattagtg ccaccgaagc cgaaggagtt acacagcgtg tattccattc
    47581 cgctgacctg acgtgcttca tgcggtacga aatccagatc gcaaccttca tccgggttat
    47641 ccaggttgat ggttggcgga acagcctgat cgcgcagcgc caggatggag tagatagatt
    47701 ctactgcacc cgccgcacct aacaggtgac cggtcataga tttcgtggag cttaccaaca
    47761 cacggcttgc agcttcaccg aagatggttt tcaccgcctg cgcttcagct ttatcgccag
    47821 ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg actcgcttca atgcctgcat
    47881 cacgcagagc atttgccatt gccagagctg cgcctgcgcc attttctggc ggtgacgtca
    47941 tatgataagc atcgctgctc ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc
    48001 gttttttcgc gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg cccagtacga
    48061 agccatcacg ctctttatcc cacgggcggc tcgccgcttg cgggttatca ttgcgggtag
    48121 ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg cgtactggct ttctctgcgc
    48181 cacctgcaac catcacgtca gcatcgccat acgcgataat acgcgcagca tggccaatgt
    48241 tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct tgggccacgc aggccataca
    48301 tgatagtcag atgacctgcc accatgttca caatcgttga cggaacgaag aatgggctga
    48361 tcttacgtgg accaccgttc atcagagatg tgtggttttc ttcgatcagt ccgaggccgc
    48421 caatcccgga gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc
    48481 cagaatcctg catggcctga acgccagcga caattccata ttgaatgaag gcatccatct
    48541 tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa atcctttact aagccagcaa
    48601 atttcgttgc ataggcgcta gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg
    48661 caagcagagc tttccaggta gactctacgg tattgccgac aggagacaac atgcccagtc
    48721 cggtcacaac tacacgacgc ttagacacgt ttgtcctcca aggagggaaa aatgattcta
    48781 gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc
    48841 ctggtggccg ttgatgtaat caatggcagc ctgaacggtg gtgattttct cagcttcttc
    48901 gtccggaatc tcagtatcaa actcttcttc cagagccatt accagctcaa cggtgtcaag
    48961 agaatccgcg cccaggtctt caacgaaaga agcattgttg gtaacttctt cctgcttaac
    49021 gcccagctgt tcgccgataa ttttcttaac gcgttcttcg atagtgctca tactcttaaa
    49081 tttcctatca aaactcgctt tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt
    49141 gcaactaaat cccggcaggt cttaccacga ttttacgtta ttttgaggcc ttacccccca
    49201 ataacgcaaa tcattttgca ctaattatta ctcataacca cgcagactgc gcgcaacatg
    49261 agcattttgt gcaaatcgcg gtcagaccat gtacatcccg ccgttcacat gcaaagtttc
    49321 acccgtgatg taagctgctt cgtcggatgc caggaatgca accgcgttgg cgatttcctg
    49381 tgcgccgccg aggcgacccg caggaacctg cgccaggata cccgcacgct ggtcatcgct
    49441 cagcgcacgt gtcatgtccg tttcaataaa gcccggagca acaacgttta cagtaatacc
    49501 gcgtgacgca acttcgcgcg ccagtgattt actgaagccg atcaagcccg ctttcgccgc
    49561 agcgtagttg gcctgaccgc catttcccat ggtaccaacc acagaaccga tagtgataat
    49621 acgaccatga cgctttttca tcatagcgcg cattaccgct tttgacagac ggaaaacaga
    49681 tgaaaggtta gtttcgataa tatcgttcca ctcttcatct ttcattcgca ttaacaggtt
    49741 atcacgagtg ataccggcat tattgaccag gatatccact tcaccaaatt ctgcgcgaat
    49801 tttttccaga acagattcga tagatgccgg gtcggtcaca ttcaacatca gacctttgcc
    49861 gttggcacct aaataatcac tgatcgcctg agagccattt tcactggtcg cagtgccaat
    49921 aactttcgcg ccacgggctg cgagcgtttc agcaattgcg cggccaattc cgcggcttgc
    49981 accggttacc agtgcgattt ttccttcaaa attcatgatt ttcctctttt aaagctcgag
    50041 cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg
    50101 tttcgtcagg ccagtaagca ccttgcccgg gccgacttca tagagatgtt ctacgccttg
    50161 cgctgccatg tactcaacag acttcgtcca ctgaaccggg ttatacaact gacgtaccag
    50221 tgcgtcacgg atggcatcac cattggtttc gcatttcaca tcaacgttat tcacaacaga
    50281 aactgttggt gcgttaaagg tgattttcgc taattctact gccagtttgt cggctgctgg
    50341 tttcatcagc gcacagtgag acggtacgct cactggtaac ggcagcgcgc gttttgcgcc
    50401 agcagcttta caggcagcgc cagcacgctc aaccgcttct ttatgaccgg caataaccac
    50461 ctgtcccgga gagttaaagt ttaccggaga aacgacctga ccttctgcag cttcttcaca
    50521 cgctttcgca atagacgcat catccagacc gatgattgcc gccatagcgc ccgtgccttc
    50581 cggtacggct tcttgcatga acttaccgcg catctcaacc aaacgcaccg catcagcgaa
    50641 atcaatcacg ccagcgcaaa ccagcgcgga gtattccccc aggctgtgac cggccatcat
    50701 tgccggtgct ttaccgccct gctgctgcca tacgcgatac agcgcaacag atgcagtcaa
    50761 cagcgctggc tgggtttgcc aggttttatt cagttcttca gctggcccct gctgggtcag
    50821 cgcccacagg tcgtagccca gcgccgcaga agcttcagca aacgtttctt cgacaattgg
    50881 atagctcgcc gccatatcag ccagcattcc aacggtttga gaaccctgtc cagggaacac
    50941 aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa cgaaccagcg cggagcccca
    51001 agtgaatcca ccgccaaagg cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc
    51061 gcgtacagct tcatccagcg cgcacgggac agaggccgca gaggtattac cgtggcgatc
    51121 cagcgtcacc acgacattgt ccatcgacat accgagtttt ttcgccgttg cactgataat
    51181 acgcaggtta gcctgatgcg gaaccagcca gtccagttga gaacggtcaa gattattcgc
    51241 cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc gcaaccttga agacttcgtt
    51301 gcccgccatc gtcagatgaa ttgaattctc tggattcacg cggtcggcgt ttggcagcgt
    51361 cagcaattca ccataactac cgtcggcatg cagatgggtg gaaatgattc ccggctcttc
    51421 agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa ataataatag tcccacgatc
    51481 tgttggatcg caggtgcgcg ccagtacatc ggaaccgacg accagagcat acttcaccgc
    51541 cccagatttc acgtattgat cggctacgct taatgcatag gtgaaacctg cgcaggctgc
    51601 tgcaacgtca aatgccgggc aacctttaat gcccaacatg ctttgaatct gacaagctgc
    51661 gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg atcaggccaa tctggtcttt
    51721 ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt
    51781 ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta cgagtgacaa tccactcgtc
    51841 agaggtgtcc accatttttt ccaaatcggc gtttgtccgc acttgttcgg gcagatagct
    51901 gccagtacca ataatcttcg tatacatgta cgctcagtca cttttcggtt atataccgtc
    51961 acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg cagagttccg cttttgccac
    52021 cgtccagcag ctcaaaacca gctgggtata cagattccag gcgagcggca attcgctgag
    52081 gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat
    52141 tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc taacagacag gcgccgttat
    52201 actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta
    52261 ataacagtag ccaccacgac cgttttttcc cttcaccctg agatttcagc agagaaagga
    52321 acatcctgac aacaccttcc atcgtcttta acgtgacatt tcctgtaaag ccgtcacaaa
    52381 ccagcacatc tgtcttgcca gttaacaact cattggcttc aagatagccg atataattga
    52441 tagaagggat tgttttaagc accgctgagg catcccgaat actgtcaaga ccctttactt
    52501 cttcttcacc aatattgagc aacgccacgc gaggattggg aatttccacc acctcttcag
    52561 ccagaactga gcccataatg gcaaattgca ccaacattgt gctgtcacaa tcgacgttgg
    52621 cccctaaatc gaggaccacc gttttgccct tttgctgatg tggtaatacc gtcaccagcg
    52681 ccggacgctc aatcccctcc aggggcttga gtaataattt tgccagcccc atcagtgccc
    52741 cggtattacc ggcactgaca caggcttgcg ctcgaccttc tttcaccagc tccagggcca
    52801 tacgcattga actcccacga ctggcgcgga tagcttgcga aggccgggca tcactggcga
    52861 taactgactg cgcaggaata atctgcagac gcgaacgttg ttcaaagtca gctttagcaa
    52921 gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag agtgagttgc gaattagaat
    52981 tcagtgcctg caatgctgca ggcactgtca cggaagggcc aaaatcccct cccatgacat
    53041 ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt
    53101 tgcctgggga aaatcctcac taagcttcat cacgcagacg cgtgattact tagcgatgac
    53161 cttgcggccg cggtagtaac cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga
    53221 agttttgtct acagacaggc tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc
    53281 acgtttggaa cgggttggtt tattctgttg tacggccatg gaccttactc ctcaattacg
    53341 gtttatcccc gatccaacgg ggagcaccaa ttacttacgc tttaagctgg ctaatacggc
    53401 aaatgggttt ggcttttgca cttcttcagg cagttcacca aagaccatat ccgcttcgga
    53461 cacttcacag tgttcagaat catgcaccgg aactaccggc aaggcgagga tgatttcatc
    53521 ttcaaccatt gcaagcagat cgatttcacc gaattcgtta acctcaatcg gttcatacgc
    53581 ttccggcagt gcttcagcct gttcgtctga acgcacagga ctaaaacaat acgttgtgta
    53641 gacctgatga gtaaacggct tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc
    53701 atcgccgttt aacactgcga gacgttggtt atcgatagcg aacgacatgg agcattccac
    53761 atcactgtcc acactgacta cggattcggc gacgcgctca acctgatcag gggtatagat
    53821 accctggtaa tcaaggcgtt tttgagccgt acgaaccgga tcgagagtca gggggaattt
    53881 taccttttgc atagggcgcg catattaact ttgtaacgtc atagagtcaa agaaaaaggc
    53941 agcctgtggt tgccttttgc caataattcg cacacattgc gggttactgc tttattttcg
    54001 ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg catagtttaa aatggctatc
    54061 atcaatacgc tatatctgat ggaaaaaaat atgcctaaac ttattttagc ctccacctcg
    54121 ccatggcgtc gcgctcttct ggaaaaactg caaatctctt ttgaatgtgc agcacctgag
    54181 gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt tggtgcttcg actggcacaa
    54241 gaaaaagcgc aatctctggc gtcacgttat ccggatcatt taattattgg ttcagaccag
    54301 gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg
    54361 caattacgca aagccagcgg caatatcgtc accttctata ctggactggc gttgtttaat
    54421 tcggcgaatg ggcatctaca aacagaagtg gagccttttg acgtccattt ccgtcaccta
    54481 agcgaggcgg agattgataa ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc
    54541 tttaagagtg aaggatttgg cattacgctg tttgagcgtt tagagggacg tgaccctaac
    54601 acgctggttg gtttaccgct aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac
    54661 ccgctgatgg gatagttgtt tgcgccggat gcggttacgt cgcatccggc aatcggtgca
    54721 ggcggcctga tgcggcgtaa acgccttatc aggccaaccg cccattatca ggcttatatg
    54781 cttgtattag cgcgcgttac gcagtttttg caaacaacgc ttcaaacctt catccatcgg
    54841 cgcttcgata cgcatcacct caccggtccc cggatgggta aacttcaacg ctgcggcgtg
    54901 caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc
    54961 accgtagcga tcgtcaaagg caatcggatg acccgcatac tgtgtatgca cacggatctg
    55021 atgagtacgt ccggttaccg ggctacaacg caccagggtg gcaaatgcat agcgttcttc
    55081 cactttaaag cgtgtttccg acggtttgcc ttcctgactg acacgcacga tacgttcgcc
    55141 gctttgcaga atatttttca ataacggcgc ttgaacgctc ttcacatgcg actgccactg
    55201 accgcgcacc agcgccaggt aatctttttg cattcctttt tcacgtaatt gctcatgcag
    55261 agaacgcaac gccgagcgtt ttttcgctac cagcaacaca cctgaagtgt cccggtcgag
    55321 acgatgaacc agttcaagga accgcgcttc cgggcgcaac gcccgcaaac cttcgataac
    55381 gccgaagctt aaaccactgc cgccatgtac cgccgtaccg gaaggtttat tcagcaccag
    55441 gatatgatca tcttcatata agatgacgtc cgccagcgcc gccacctttt gcagatgtgg
    55501 cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt gggatacgca cctcatcacc
    55561 cgcttcgagt ttatattcag gcttaatacg ttttttgttc acccgcactt cacctttacg
    55621 caaaatacgg taaatcatac tttttggtac gcctttcaat tgggtacgca aaaagttatc
    55681 gatacgttgc cccgcttcgt cagcggtgat agcaacaatt tttacggatg gagtctctgt
    55741 tttcatggtg ggcgattcta attagccaac aggattcgcg ccactcattt ttctatgctt
    55801 atatttagtt ttgcacctta ttacttcact acgtgatcac tttattgatg gttattaaac
    55861 caatcaccag caagaagtga aaaaactgtg agtaagcggg tgataaatgg taaaagtcat
    55921 cttgctataa caaggcttgc agtggaataa tgaggccgtt tccgtgtcca tccttgttaa
    55981 aacaagaaat tttacggaat aacccatttt gcccgaccga tcatccacgc agcaatggcg
    56041 taagacgtat tgatctttca ggcagttagc gggctgcggg ttgcagtcct taccggtaga
    56101 tggaaatatt tctggagagt aatacccagt ctgtttcttt gataattgcg ctgtttttcc
    56161 gcatgaaaaa cgggcaaccg acactctgcg cctctttgag ctgacgataa ccgtgaggtt
    56221 ggcgacgcga ctagacacga ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc
    56281 agcttagtcg tcaatgtaag aataatgagt aagttacgat gaaaagaatg ttaatcaacg
    56341 caactcagca ggaagagttg cgcgttgccc ttgtagatgg gcagcgtctg tatgacctag
    56401 atatcgaaag tccagggcac gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc
    56461 gcattgaacc gagtctggaa gctgcttttg ttgattacgg cgctgaacgt cacggtttcc
    56521 tcccactaaa agaaattgcc cgcgaatatt tccctgctaa ctacagtgct catggtcgtc
    56581 ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat tgttcagatc gataaagaag
    56641 agcgcggcaa caaaggcgcg gcattaacca cctttatcag tctggcgggt agctatctgg
    56701 ttctgatgcc aaataacccg cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc
    56761 gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg
    56821 tgcgcaccgc tggcgtaggc aaatctgctg aggcgctgca atgggattta agcttccgcc
    56881 tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg cccggctccg ttcctgattc
    56941 atcaggagag caacgtaatc gttcgcgcct tccgcgatta tttacgtcag gatatcggcg
    57001 aaatccttat cgataacccg aaagtcctcg aactggcacg tcagcatatc gctgcattag
    57061 gtcgcccgga tttcagcagc aaaatcaaac tgtacaccgg cgagatcccg ctgttcagcc
    57121 actaccagat cgagtcgcag atcgagtccg ccttccagcg tgaagtccgt ctgccatccg
    57181 gtggttccat tgttatcgac agcaccgaag cgttaacagc catcgacatc aactccgcac
    57241 gcgcgacccg cggcggcgat atcgaagaaa ctgcgtttaa caccaacctc gaagccgccg
    57301 atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg cctgattgtt atcgacttca
    57361 tcgacatgac gccagtacgc caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc
    57421 gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg ctttggcctg ctggaaatgt
    57481 cccgccagcg cctgagccca tcactgggtg aatccagcca tcacgtctgc ccgcgctgct
    57541 ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct ctctattctg cgtttgatcg
    57601 aagaagaagc gctgaaagag aacacccagg aagttcacgc cattgttcct gtgccaatcg
    57661 cttcttatct gctgaatgaa aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg
    57721 gtgttcgctg cgtgattgtg ccaaacgatc agatggaaac cccgcactac cacgtgttgc
    57781 gcgtgcgcaa aggggaagaa acgccgacct taagctacat gctgccgaag ctgcatgaag
    57841 aagcgatggc gctgccgtct gaagaagagt tcgctgaacg taagcgtcct gaacaacctg
    57901 cactggcaac ctttgccatg ccggatgtgc cgccagcgcc aaccccagct gaacctgccg
    57961 cgactgtcgt agccccagca ccgaaagctg caacggcaac accagcagct cctgcacaac
    58021 ctgggctgtt aagccgcttc ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag
    58081 ccaaaccgac cgagcaacca acaccgaaag cagaagcgaa accggaacgt caacaggatc
    58141 gtcgcaaacc tcgtcagagc aaccgccgtg accgtaatga gcgccgcgac acccgtagtg
    58201 aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc
    58261 agcaaactgc cgagacgcgt gagagccgtc agcaggctga ggtaacggaa aaagcacgta
    58321 ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac
    58381 gtcaggcgca acaagaagcg aaggcgctga atgttgaaga gcaatctgtt caggaaaccg
    58441 aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga
    58501 aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa
    58561 ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc acgcacagaa ctggtgaaag
    58621 tcccgctgcc agtcgtagcg caaactgcac cagaacagca agaagagaac aatgctgata
    58681 accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga
    58741 gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt
    58801 tgaccgtagc gtgcgcgtct ccggaactgg cctctggcaa agtctggatc cgctatccaa
    58861 ttgtacgtcc gcaagatgta caagttgaag agcagcgcga acaggaagaa gtacaagtgc
    58921 agccgatggt gactgaggtc cctgtcgccg ccgctgtcga acctgttgtt agcgcgccag
    58981 ttgttgaaga aatggctgaa gtcgtagaag cccccgtacc ggttgccgaa ccgcaaccgg
    59041 aagtggttga aacgacgcat cctgaagtga ttgccgccgc ggtaactgaa cagccgcagg
    59101 tgattaccga gtctgatgtt gccgtagccc aggaagtcgc agaacacgca gaaccggtgg
    59161 ttgaaccgca ggaagagacg gcagatattg aagaagttgc cgaaactgct gaggttgtgg
    59221 ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag
    59281 aagttgaaac ggtagccgcg gttgaaccag agatcaccgt tgagcataac cacgctaccg
    59341 cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt cacagtgact
    59401 ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat acggcaacgc
    59461 atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta gctcaaagta
    59521 atcaagccct ggtaactgcc agggcttttt tatttcatgt ttgaatcaac atattaaaat
    59581 tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaaa tgcccgcccc
    59641 ggcgatccca gagcgggcag acaaaacata cccagtttca tgatatgttt taaagcgagc
    59701 ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt tatacgatgc
    59761 ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt ccacatcaac
    59821 cagatcgctc atctgctgcg tttgccctaa agcgcgatcg ctacctaagg aatccagcga
    59881 ctccagtttg ttcagctgcg tgcctaattc cgtgcgaacg gtaaggacat tattcagcga
    59941 gtttttcagt ccacggttgg ttttatccaa tgccgcagcg gcgatttctt tatccgcttc
    60001 gctatccgcg accggcgttt tcagggctgc gatggcgcta tccagcatgg caaaaagatt
    60061 ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta ctggtaatac tgtcgaaaat
    60121 tttgtcgccc gtatgtccta tcaccatcga acgcgaagca tcgacctgtt gcttaatact
    60181 ttctgttccg ccgacataat caccgttagc ctcactaaac ggcgcagtct ctgttttata
    60241 accggcaaaa atgtagcgcc cattaccgtc agtggtgttc gccagattca gcaactggtc
    60301 acgaagcccc tgaatatccg tagccagcga ggcccggtca tcgtcactca aggtgccatt
    60361 gctggcgtag acaatttttt cctgagcatt ctggatagca gtggtgacct ggctaagtac
    60421 actctcttcc agtgacactt tttgagtggc gaaagtacgt gccagcgtgt actggctgtt
    60481 ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca gcaatgggat cgtctgaagg
    60541 gttaacgact cgcttacccg tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga
    60601 attggtgatg ccgcgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc
    60661 tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc
    60721 gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc cgtattcctc atcgagattg
    60781 acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg
    60841 ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg
    60901 tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc tttgcagatc cagcagggcc
    60961 tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc gattttcgct
    61021 tcgtcggtga ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag
    61081 ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta
    61141 ccgttggcat ctggtgtcgc cgtaaaagtg gtattgctgg caaggcgggt gacctgccac
    61201 tgattattat cgaacgagat tttgtaatct gtcgccagta ccacggaggc atcagttacc
    61261 gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt gctgaaccgc gggattaccg
    61321 atagtaaaga aatcttcacc agcatcgccg ttagcatcaa atccggcttt gtgttggctg
    61381 ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt ctggtccaga
    61441 tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagtaa tttctccggg
    61501 atctcaatat tgcctgccgt cccatcaacg taagcgacag tcgtacgaga agggtcggcg
    61561 ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg
    61621 gcatcgtgat gttataagta ccgccatcct gaacgctgac ttctacgcca acaatctggt
    61681 ttaattcgct caccagttga tcgcgttgat ccagcagatt gttaggtgac gcccctgccc
    61741 ccacgcctgt caggcgcgag atttgatcgt tcaggctggc aatttgttta gcgtaattgt
    61801 tgatctgatc aacgctggca cctatcgcga tattgacctg tttgtcctgg tcgcgcagat
    61861 attgatcggt ggttttaaac tgattcacca acccttctga tttcccaatc agcgcctggc
    61921 gcgccgccgg gtcttccgcg ttactcacca gcgtttgcag gctggtgaag aaatcctgca
    61981 tctgtgttgc cagcgaagag gtactggtgg agagcatatt gtcgattttc gacatctgct
    62041 catagcgggc ggtcaggccg ctactttgcg tctgcacagc acgtaactgg ttggtaataa
    62101 acgtatcata ctcacgctgc acaccagaaa cgtagacgcc attgccaacc cagccgccag
    62161 cgcccaacgt gctattggcc tgcgccataa tagtggtttg gcgggtatat ccggcaacgt
    62221 tatagctgga gatattatta cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc
    62281 tcatggcgtt attgatcaag ctggacatgg aggttcctta taagccttca attacagagt
    62341 attatcggca gcgacccgcc ggacttgagt tattcagaac agattatcaa tgttcatgct
    62401 gtaggttttg ctcacctggt cgcttatcga tttcatctgc tggatcatgt tggtgagttt
    62461 acgggcatag tgaggatcgg tagcatagcc cgcgtcctgt aggacctgcg ccccctgttc
    62521 cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta
    62581 atccgacaat gcttccagat acgagctgta gacccgaaac ttcgctttta ctttcttcgc
    62641 ttcgccgttt tcatattcag tcgtggtgat ttcagtgact tgccctttcc agttgccaga
    62701 ggctttgaca ccaaacaggt tatagcttgg ctcgccgttt tcgcggcgga tttgccgttg
    62761 cccccaacca gattcaagcg cagcctgagc gaggatcaaa tgatgtggca caccgctttg
    62821 ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg aatgctctac tgtcacccgg
    62881 cagcgaatca tcgtagttac gtggtacggc cttttgcacc agctgcgaaa gcgtctggtt
    62941 ttgataacgc accacggttt cgagcgggaa tttcatcggt gctgctggcg tggactcctc
    63001 tggcaatggt tgttctggcg tcatctgttt aaccatcatc tctgcaagcc ccagaccttt
    63061 gcccgccgtc atctgttggg caatttgctg gtcatacata ctggtataca gacgagtgtg
    63121 ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc atgcttttca acatcatctg
    63181 cacgaacatc ccttccacct gacgggccac cggacggata tttgccgccg gatcttcgct
    63241 cgctttcgcc tttagttcgt tgagtgattg cgcatcccag gccgcacttg ccagtagttt
    63301 gctgtcgctg atcatcagat gatttccagt tttgcccgca gacatcccgc actttgcatt
    63361 gattgcaaaa tagacatcag atccatcggc gtagcgccca gcgcattgag cgcacgcacc
    63421 acgttattga ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa
    63481 tcgatctgcg tttgtggagt aacaacagtc tgtccaccac caaacggtgt atctggctgg
    63541 ctgacattgg cctgacgatt aactgttact gagagatttc cctgcgctac cgcgcagctg
    63601 tcgagggtca cttctcgatt catcaccacc gaaccggtgc gcgagttaat cactacttta
    63661 gcgtcctgcg gggtgacatt aacctgcata ttctggatat cggcaaggaa acggacctgg
    63721 gaactgttgc cacttggtac gcgcacctga atagtccgcg catctaacgc ggtggcgctg
    63781 ccatatccac gcacgcggtt gatggtgtca gcgatttgct gcgccatgct gaaatcttcg
    63841 tcgttaagtt gcaaattaag ggtattcccg acgccaaact ggctgggcaa ttcacgttca
    63901 ataaccgcac cattggtgat ccgtccaccg ttcagttggt taacctgaac actgctaccg
    63961 cccgcggagg ctcctgcgcc gccaaccaga atattgccct gcgccagcgc atacacctga
    64021 ctgtcaacgc ccttaagcgg tgtcatcaac aacgtacctc cacgcaagct tttggcatta
    64081 cccatggaag aaaccaccac atcgatggtt tgcccctgac gtccaaacgg aggaagtgac
    64141 actgtcacca ttaccacagc gacgtttttt agctgcatat tggtgcccgt cggaacggta
    64201 attcccagct gtgagagcat gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc
    64261 tggtcaccag tgccatccag ccccactacc aggccatagc caatcagtga gttttgcctt
    64321 accccctgaa cactggtgag atcgcgaata cgctcagcct gagccgccgt cgtaaccagt
    64381 agaagaatta atgcagagag aaatttaatc accacagcct cacttacatt ggcgacaggt
    64441 taaggaagaa acgctgcaac cagcccatat tttgcgcttc gttaatgtag ccattgccta
    64501 cgtattcaat acgcgcatcc gccacctgag tagacggtac ggtattgctg ccgctgatag
    64561 tgcgtggatt aaccacgccc gagaagcgaa taaattcggt accctgatta atggcaatct
    64621 gtttttcacc caccacatgc aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca
    64681 acgtgccgct aaaggtattg ctggcattgg ccccgccctt tccgttgaac gtgttaccac
    64741 cggaggcttc gacatcggca cgagcgttac caaacagccc ctgcaaatag cgcggcacag
    64801 tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt cgcagaggag cttttgctgg
    64861 cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat
    64921 cttcaaacag cggttgatag ccatagttaa tcggctgagc agactggaaa atagaaccgt
    64981 tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc accagcggcg
    65041 tggagggtat ccaggcgcag ccggttagtg aaagcaccaa caagctggaa atggcataag
    65101 tatgcgcagc gtttttttgc atggctatca tcttcaaaaa tcagtaaacc ggtgaacctg
    65161 ccaccggtta tgccttagag ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc
    65221 gctttactgt tgatttcgta agcgcgttgc acctgaatca tattgaccag ttcttccgcc
    65281 acgttgacgt tagacgtttc aacataccct tgatacagca gtcccgcgcc gttcaggccc
    65341 ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt ttcgccaatg
    65401 ctctccagtc cggtatcatt cataaaggtg gtgagattga gctgcccaac ctgaaccgga
    65461 gctgcctggc cttgttgggt tacgctgacc acgccatcac gaccgatggt gatacttaac
    65521 gcattcgccg gaatggtgat cgctggctgc acctgaaaac caccagccgt caccagctgc
    65581 ccgttctgat ccacctggaa agagccgtca cgggtatagg ctgatgagcc atctggcaac
    65641 attacctgga aaaagccctg ccctttaatc gcgacatctt tgctgttatt ggtctgcgac
    65701 agattgccct ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt gccgatttgt
    65761 aatccggagg gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg aatggtttga
    65821 taaagcagat cttcaaacac cgcgcgctga cgcttaaaac cgttagtact gacgtttgcc
    65881 aggttgttgg caatgacgtc catattggtt tgctgggcgt caaggcccgt tttggcgatc
    65941 cataatgaac tgatcatata atgtcctgta gtggattgct gacaagcaga gcagaagcac
    66001 gcgacagtgt gaatttcccc tcaccctaac cctctcccca aaggggcgag ggaatagtcc
    66061 gcgcgctttt attgcaatgt catcaatcgt tcaattaact catcgacagc agttggttgg
    66121 cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca aaacgccgcg
    66181 cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct tccagaaccc
    66241 ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg ccctcgcgtg gcctgggttt
    66301 ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc gtggctttca
    66361 ccagtttcag acgccctact ggcgcaactg tatttgccgg atcgcccgga ttgagcgccg
    66421 agattgtgcc atcggcagca atcgtgattt ccgccccttc cggcacagca attggccccg
    66481 cctcgcctat caccggatgc ccctgaattg tcagttgccc ggtgggatca acctgaatgc
    66541 tgccattacg cgtataccct tcgctgccgt cagcggtctg cacggccagc catcctgctg
    66601 caacgcaacg tccagcgggc gcgaggtgta atccatcttg ccgggcgtca tgtctgcacc
    66661 cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc
    66721 gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag gcattggcca gattactggc
    66781 ggttaccgcc tgttgattca gcgtctggct ggctgctccc atcgcagtat aaattgcgtg
    66841 atccattgag ctatcccgtc agcgattagc gtaagttaac ccgcgtgttg aggatctggt
    66901 cctgggtttt gatggtctgg gcgttagact gatagttacg ctgggcaacg atcatattga
    66961 ccagttcttt actgagatcg acgttggacg cttccagcgc accgttggtc agggtgccaa
    67021 agtttcccgt cccggctgtc cccaacagcg ccacgccaga agattgcgtc gcagaccaga
    67081 cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt acaatctgcc
    67141 ccagcagttg ggtttgttcg ttggaatagt tgccgacaac cgtaccgtca tcattgattt
    67201 gataactcac cagatcgccc ggtttgtagc cattctgggt ggttgccaca atgttgttag
    67261 cgccggtatt ttgctgcatg gagttgagga agctcagact aaacgtggcg ggttctgcac
    67321 cgttaattgc gccggttgcg atattattcg ccatcgcaca tgtgccatta gcattaaatt
    67381 ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact gctatcctgg gtgtagacct
    67441 gccagttatt atccccggtc ttcacaaaat agacgctcat gtcatgagca ttaccctgac
    67501 tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgctg gcgctgaatg
    67561 gcgtaacagt aggaagcgga tcactggaat tcaggttgat ctgcatcgac gccgtggtgg
    67621 tagttttcgc tgccatcagg gtattcggga tcgaaatatt ggtcggattc gccccttgct
    67681 gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag ctgtaaacct tgcatattca
    67741 acaggttacg gttttcatcc agcttaaatt gtccgttacg gctgtagaac accgaaccgt
    67801 tgctgtctac cagacggaaa aaaccgttct ggctgatagc aacgtccaga cctcgcccgg
    67861 tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt acccccagtc
    67921 ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta aagccgtagg
    67981 tggcggagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca gcgtttaatc
    68041 cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct taaattatct
    68101 gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag atccagcgta ttaccgctgt
    68161 tgccgcggat cacaccctgc accagagcaa actgcagcgg ctgggcaacc agttgtgtac
    68221 caccgttact ggcgctaatc gctacattgt aagaaccgtt cggcgcagtg ctgccatcag
    68281 tcaacgtacc gtcccaggtg aaactgtgaa ctccggcggt cagttcacca atatcaatgg
    68341 tgcgcacaac cgcgccattt ttatcggtga tggtggccgt aactttgtct gccgcctgtt
    68401 gcagctcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg ccggttcccg
    68461 caagaacagt tgtgccgggg atcatcacgc cgtgaccgat caggttactg gcctgtaacg
    68521 actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt ttttcaatcc
    68581 cgctgaccgt gctgatttgt gccaattgcg acgtcagctc gttgttttcc attggattgg
    68641 tcgggtccta gtttttcagc tgcgccacca gcaaagtcag aaaactgctt tgtaaatctg
    68701 cggcgttgct gcccgtgagc gaactactgc tggtggtact gacgccggta tttgtcggat
    68761 cggtggtggt taccgcaatg gacatagctt tctcctttat tgaccgagcg taagggtttt
    68821 cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgatagc tgcgtgacgc
    68881 cgacatggtg ttaaccatct ccccgacgac atcaacgttc ggcattttta cgtagccctt
    68941 tgcatctgcc agcggattac ctggttcata aaccagtttg tccggggcct gactttctta
    69001 acatcggcaa cctttacgcc gcctgtcgca gcacctggtg cagcgttaac ctggaatacc
    69061 acctgttttg cccgatatgg ctgtccatca ggaccggtca cgctatcagc atttgccaga
    69121 ttactggccg ccacgttcag gcgctgggac tgggcagtta acgccgaccc ggcgatatca
    69181 aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat catgcctttg
    69241 atttgcccgc tcaacgcgct aaggctcatc tggtattgca ggctgttatc ggcaaactgg
    69301 gtgacttcct gacgctgggc gcgcagattg agcgcctctt gttgaaaacg taaggcggcg
    69361 tcgagcttat cgagcatatc tcctccgcag gtatcaaaat tctgccatca gcttaaatgc
    69421 ctttacgcgc gcgttatcgg cggaataaac gcaaaatggg tcgctattta tgccgttgat
    69481 ggtcattgcg gacaggtaca actcacgttg tagaaatggc tgggggcgaa aatgctgaca
    69541 ataaaacgta gcgtggcgat catcgcgata ctgttcagtc cgttaagtac ggcgagcaat
    69601 ctcacatcgc aattgcacaa cttttttagc gcccaactcg cgggggtaag tgatgaggtt
    69661 cgtgtttcta ttcgtacagc gcccaatcta ctaccgccat gcgagcagcc attgctttcg
    69721 atgagcaata attcccgcct gtggggcaat gtgaatgtgt tggcacgctg cggtaacgac
    69781 aaacgatatt tacaggttaa tgtgcaggcc acaggaaatt atgtggttgc cgcgatgcct
    69841 attgtgcggg gaggaaagct ggaagctggc aatgtcaaac tgaaacgtgg acgcctggat
    69901 accctgccac cgcgtacggt gctggatatc aatcaacttg ttgatgccgt tagcctgcgc
    69961 gatctatcac ccgatcaacc tatccagtta acccagtttc gccaggcatg gcgggtaaaa
    70021 gcgggacaac gcgtcaatgt gatcgccagc ggtgatgggt ttagcgccaa cgcagaaggt
    70081 caggcgctga acaatgcagc cgtcgcacag aatgcgcggg tgcgcatggt atcgggacag
    70141 gtagtcagcg gcgttgttga tgcagatggg aatattctta taaacctgta agctgttaaa
    70201 gattacccgt cccttgccga taaataagca acacatgata aaagcgccct caatgaggaa
    70261 taaaccatga gtattgatcg cacttcgcct ctgaagcctg taagcaccgt tcaaccgcgc
    70321 gaaaccactg acgcgccggt aacgaacagc cgcctccacc agcaccagtg tgacgttaag
    70381 cgacgcgcaa gcaaaactga tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga
    70441 agcgttaaaa ctggcgattc gtaacggtga actaaaaatg gacaccggca aaattgccga
    70501 tgcgctgatc aacgaagcgc agcaagactt gcagagtaac tgatcgtatg acacgtcttg
    70561 cagagatcct cgaccagatg tccgctgtgc ttaacgatct caaaacggta atggatcatg
    70621 agcagcaaca tctctctatg gggcagatca acggcagcca gttgcaatgg attacagaac
    70681 aaaaaagctc actgctggcg acgctggatt acctcgaaca gttacgcaag aaagaaccca
    70741 atacagcaaa tagcgttgat attagtcaac gctggcagga aattactgtg aaaacgcagc
    70801 aactacgcca aatgaatcaa cataacggct ggttactgga aggacagatt gagcgcaatc
    70861 aacaggcgct ggaaatgttg aaaccgcatc aggaaccgac gctatatggg gcgaacggtc
    70921 agacctcaac accccatcgc ggcagtaaaa agatttcgat ctgaatatta tctccggcct
    70981 gcactgcagg ccggaatgca ttgttacacc gtccggcggg caaattcttt aactttgaag
    71041 cccagtaccg ccagtgcagc gaagtacgcg gcaatccccg ccagcacgac cgccattaaa
    71101 cgcagtaagc gccagggcat ggtacccaat gaccactccg gcatgatatg taacatacct
    71161 aaaagcacgc cagacatcac cagtaccgcc accaccagac gcaacagaaa cgccatccag
    71221 ccgggttgcg gagtaaagat tttctgctta cgcaactgcc agtaaagcag cgaagcattc
    71281 agacacgccg ccagaccaat agaaagtgac agcccggcat gtttcaacgg accaataaac
    71341 gccaggttca tcaattgcgt taaaatcagc gtaacgatgg caattttcac tggcgtttta
    71401 atgtcttggc gggaataaaa gccaggagcc aacactttca ctacaatcag gccaatcaaa
    71461 cccaccgagt aggcaattaa cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta
    71521 ccgtactgga acagcgaaac ggttaacgga cctgaaagaa tgcccaacgc aaccgcactc
    71581 ggcagtgcca acaggaaaca aagacgcaac ccccagtcca tcaaacggtt gtattcatca
    71641 tgattgccac tggcaaaact tttcgacagc gacggcagca aaatggtgcc aagcgccacg
    71701 cccagcacac cggagggaaa ctccattaag cggtcggcgt aatacatcca agacaccgaa
    71761 ccggaagcaa gaaacgaggc aaaaatggtg ttgatgatta aggagatctg gctcacagag
    71821 acgccaagga tcgccggtcc catctgtttc accacgcgca ttgctccagc atcgtggaag
    71881 ttaatgcgcg gcaggaccag catgccgatc ttcttcaggt gcggtagctg atacaccagc
    71941 tgcaggatgc cgccgaccgt aacagcccac gccagcgcca gcaccggtgg gttaaagtac
    72001 ggtgcggcaa acagcgcgaa accaatcatg ctgatgttaa gcagtgttgg cgcaaacgcc
    72061 ggaatcgaga agcggttcca cgtattcaga atcgctccca ccagcgacgc cagggagatc
    72121 agcaagatat agggaaaggt aatctttagt aggtggctgg tcagggcaaa tttgtcagct
    72181 gtgtcagcga agcctggcgc ggtcaccatg atcacccacg gtgcggcgag catgccagcg
    72241 accgtcacaa ccgccagcgc aagtgtcagc aggccagaaa cataagagac aaagacccgc
    72301 gtggcgtctt caccctgctt acttttatat tccgccagaa tcggtacaaa tgcctgggaa
    72361 aatgcccctt cggcaaagat acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag
    72421 gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca
    72481 agcacacgcg aaaacatggt catcgagctg acggcggcca gcgattttaa taaattcatc
    72541 ggtgttctaa tccggaccca cgggcaaaac gcctgcaagg caggcgctga aagaggcgaa
    72601 tagtctacct agattatggt gaattgctac cgccagatgt tacagggttt attcactcat
    72661 cgcatcgcgc cagatcttgt caacgatacg ttgcgccagc actgcctatt cgccggcggt
    72721 ttgcggaact gtctgatttt gcacgcattc aatgaagtgc cgcgcacagc cgacaaatcc
    72781 acgttgctca agagtgctct gccaaccagg aatcggttta ttcactacac cctgcccgcg
    72841 ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg
    72901 cacggtttca cgctgacttc cggcacggcg atgcatacaa gtggtgatct gcaaaggacc
    72961 ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg tcgttagtca gtagcgtacc
    73021 gccatccaga gaggctttgc cgcccgacaa ccacagcgcg gtatccacca catgcagata
    73081 atcatccagc aacgtgaaat aaagatcgtg tggcccgacg ctattgctac gatgtttatc
    73141 cattcttagc gaggctgcgg tggcgagttg cgtttttaac tcaccgtaga gtggtgcgaa
    73201 acgacggtta aaaccgacca tcaacgtcag ttttttccgc gccgccagtt ccaccagcct
    73261 ttcagcatcg cgcagatttt ctgccagcgg tttatcgaca cagacatgta cccccgcatt
    73321 gagtaacgta ctgaccacgt caaagtggct ggcggtgctg gagtgcacaa aaaccgcatc
    73381 acaactggcg gcaaggctgg ataacgaatc ggcataagga atgcgccagc tgtcacaaat
    73441 tggcagggct ttggcgcgcg taggcgacca ggctccttgt aacgtccagt cagacgctgc
    73501 cgccagcacc ggtaaccacg ctttttgcgc aatgccgcct aatcccacta cgccgatacg
    73561 taattttttc actttaatct cccagatggg ccagcaacga atcaagacgt tgtttcagtt
    73621 ctgccacttc gatttccagg gcttcgacgc gggtctgtaa atcaccgtca accatgttcg
    73681 acatagccgt taccgccggc tgatcttcaa cctcaccact gaaaagatgc atgtagcggc
    73741 tttcgcgttt acccggttcg cgggccagac gcaccacaaa aggaccatct tcgcgattcg
    73801 ccagttgttc cagcgtcgac tccacttccg ccatatcgct gaattcatac attcgcgcgg
    73861 cgcggctgcg cagttcaccc agcgtctggg caccacgcaa taacaacgtg gtgattaacg
    73921 ccacttctgc ggcgctcagt ttcagatcgc caaattctga attacaaaaa cgttgctcat
    73981 atttggtgac ccgattacca aaaccgctca ctgtgcgtag ataatgacgt ttgaccagat
    74041 tatccagctg ttcctgcact tcggattcac tcagattcat gaccggttca cggttcgttt
    74101 tctgattaca ggccgtgact acaccattga ctgagagcgg gtattgctcc ggcgtggtca
    74161 cctgcttttc cagcaagcaa ccaattacgc gagcttccag ggcagtaagt tgatatttca
    74221 tgagattctc cttagcggcc gggcgtccag tctggggtag ttaatgccgt cagtacgtga
    74281 tcgcgccatt gtccatcaat caacagatag tctttcgcat agccttcttt ttcaaaaccc
    74341 agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca tataattagc cataatgcga
    74401 tgaatatgtt gggtgcgctg catataacga atggctgcgg tcagggcttc aaacatgagt
    74461 cctttgccct gccatttttg cccaatcgaa taaccgagat agcaggcatg aaaagagccg
    74521 cgaacaacat tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg gtcgaataag
    74581 ccaaagtaga aagctgaacc ttgtttatga aattcgttaa tcatccctag cctggcctgc
    74641 cagcctgatg gataacagtg gctttcgtcg cgcactggct cccagggctt gaggaaatgg
    74701 cgattctctg cgtaataatc cgccagacgc caggcatcac gatcatgcac cagacgcacg
    74761 accagtcggt ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc aaacattcta
    74821 tacctactcc ttcccgtaac gtctttaata cgctgtaagt attactatac ctgcgcaacc
    74881 agaccctgtg aaaagtccaa aatgccattt tctgcattat tcacatattt gataaaaact
    74941 ctctatcaaa gcgacgtttt gataaaaaaa tattgtcccc gaagtgatgg gaaaagtctt
    75001 agcggcaatg cagaatagaa agtgctcatc ttttatttcc cgggagggga aatgtcccgc
    75061 gtatcgcagg cgaggaacct gggtaaatat ttcctgctca tcgataatat gctggtcgtg
    75121 ctggggttct ttgttgtctt cccgctgatc tctatccgct tcgttgatca aatgggctgg
    75181 gccgccgtca tggtcggtat tgctctcggt ctacgccagt ttattcagca aggtctgggt
    75241 attttcggcg gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt taccggtatg
    75301 ctgatgcgcg ccgccggatt cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg
    75361 ttttcatgcc tgctctcggg actcggcggc acgttgtttg atccgccgcg ttcggcgttg
    75421 gtggtgaaac taatccgtcc acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag
    75481 gacagtgcca gtgcggtcat tggcgcattg ttggggagct ggctgttgca atacgacttt
    75541 cgcctggtct gcgccacagg ggccgttttg tttgtgctat gtgcggcgtt caatgcgtgg
    75601 ttgttaccgg catggaaact ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc
    75661 gtgatgcgtg acaagcgttt tgtcacctat gtcctgacgc tggcgggtta ctacatgctg
    75721 gctgtacaag tgatgctgat gctgccaatt atggtcaacg acgtggctgg cgcgccctct
    75781 gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct ctaccctatc
    75841 gcccgctgga gtgaaaagca ttttcgtctg gaataccggt tgatggctgg gctgttgata
    75901 atgtcattaa gcatgatgcc ggtgggcatg gtcagcggcc tacaacaact tttcaccctg
    75961 atttgtctgt tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct
    76021 tcgctggcgg acgcaagagc tcgcggcagc tatatggggt ttagccgtct gggtctggcg
    76081 attggcggcg ctattggtta tatcggtggt ggctggctgt ttgacctggg caaatcgacg
    76141 caccagccag agcttccgtg gatgatgctg ggaattattg gcatcttcac tttccttgcg
    76201 ctgggttggc agtttagtca gaaacgcgcc gcgcgtcgtt tgctggaacg cgacgcctga
    76261 tttgatacac ccccctgact cctccatact gaaagtgaac tgacagccaa atggaggagt
    76321 cacgtgaagc tatacattta cgatcactgc ccttactgcc tcaaagcccg catgattttc
    76381 ggcctgaaaa atatccccgt cgaattacat gttctgctca acgacgacgc agaaacaccc
    76441 acccggatgg tcggtcaaaa acaggttccc attctgcaaa aagatgacag ccgctatatg
    76501 ccagaaagca tggatatcgt tcactatgtc gataaactcg acggcaaacc gttactgacc
    76561 ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg tcaatggcta cgccaacaaa
    76621 ctgctgttgc cgcgttttgc caaatcggca tttgatgagt tttctactcc cgccgcgcgc
    76681 aaatatttcg tcgacaagaa agaggccagc gcgggtaatt ttgccgacct gctggcccac
    76741 tctgacggtc tgattaagaa tatcagcgat gatttgcgtg cgctggacaa actgatcgtc
    76801 aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata ttcagctatt tccgctactg
    76861 cgtaatctga cgctggtagc cggaatcaac tggccaagcc gcgttgctga ttaccacgat
    76921 aatatggcga aacagacaca aatcaatttg ttatcatcaa tggcgattta atcctgaccc
    76981 gccgcggcag cctgtattcg cgggttgccg ccgttttcag ttttttctgc tggcgagttt
    77041 atcgctggtg acacatgctg cgttgtgtca ggatattgac gcagatttca tgtcgaggac
    77101 taccatgaac aagtttttat ttgccgctgc attgatcgtc agtggcctgc tcgttggctg
    77161 taatcaactc acccaataca ccatcaccga acaagaaatt aaccagtcgc ttgcgaaaca
    77221 taataatttc tcaaaagata tcggtttacc cggcgtggct gacgcccata ttgttctgac
    77281 aaacttgacc agccaaattg gtcgcgaaga gccgaataag gttaccctaa ccggagacgc
    77341 caatctggac atgaactccc tgttcggtag tcagaaagcg accatgaaac tgaagctgaa
    77401 agcgctgccc gtgtttgata aagaaaaagg tgcgatcttc ctgaaagaga tggaagtggt
    77461 cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg caaacgttgc ttccctattt
    77521 gaaccaggca ttacgcaatt actttaacca gcaacctgct tacgtcctgc gcgaagatgg
    77581 cagtcagggc gaagcaatgg cgaaaaaact ggcgaaaggc attgaagtga agccaggtga
    77641 aattgtcatt ccgtttacgg attaatgacg agggcgcatt cgcgcccttt atttttcgtg
    77701 caaaggaaaa catttccgct tatcctttgt gtccggcaaa aacatccctt cagccggagc
    77761 atagagatta atgactgcac catcccaggt attaaagatc cgccgtccag acgactggca
    77821 ccttcacctc cgcgatggcg acatgttaca aactgtcgtg ccgtatacca gcgaaattta
    77881 tggacgggct atcgtaatgc ccaatctggc tccgcccgtg accacagttg aggctgccgt
    77941 ggcgtatcgc caacgtattc ttgacgccgt acctgccggg cacgatttca ccccattgat
    78001 gacctgttat ttaacagatt cgctggatcc taatgagctg gagcgcggat ttaatgaagg
    78061 cgtgttcacc gctgcaaaac tttacccggc aaacgcaacc actaactcca gccacggcgt
    78121 gacgtcaatt gacgcaatca tgccggtact tgagcgcatg gaaaaaatcg gtatgccgct
    78181 actggtgcat ggtgaagtga cacatgcaga tatcgacatt tttgatcgtg aagcgcgctt
    78241 tatagaaagc gtgatggaac ctctgcgcca gcgcctgact gcgctgaaag tcgtttttga
    78301 gcacatcacc accaaagatg ctgccgacta tgtccgtgac ggaaatgaac ggctggctgc
    78361 caccatcact ccgcagcatc tgatgtttaa ccgcaaccat atgctggttg gtggcgtgcg
    78421 tccgcacctg tattgtttac ccatcctcaa acgcaatatt caccaacagg cattgcgtga
    78481 actggtcgcc agcggtttta atcgagtatt cctcggtacg gattctgcgc cacatgcacg
    78541 tcatcgcaaa gagagcagtt gcggctgcgc gggctgcttc aacgccccaa ccgcgctggg
    78601 cagttacgct accgtctttg aagagatgaa tgctctgcag cactttgaag cattctgttc
    78661 tgtaaacggc ccgcagttct atggcttgcc ggtcaacgac acattcatcg aactggtacg
    78721 tgaagagcaa caggttgctg aaagcatcgc actgactgat gacaccctgg tgccattcct
    78781 cgccggggaa acggtacgct ggtccgttaa acaataaaaa tcgtagcttc ctgttgtcat
    78841 taggttattt tacctgtata aataaccagt atattcaaca gggggctatt atgcgaattg
    78901 aagtcaccat agcgaaaact tctccattgc cagctggggc tattgacgcc ctggctggcg
    78961 aactttcccg ccgtattcag tatgcgtttc ctgataatga aggccacgta tcggtacgtt
    79021 atgccgcagc gaataattta tcggttattg gcgcaacaaa agaagataaa cagcgcatta
    79081 gcgaaattct ccaggaaacg tgggaaagcg ccgatgactg gtttgtcagc gaataatatg
    79141 cagtgatttt tgtttttgcc gggtcgcccc ggctttttta ttttacttac gaattttgag
    79201 tacaaaattt ctacacttcc tcaaaataat tgcacaatat gctaaaaaaa tatgaatgac
    79261 atgctgtttt attgttcata taaatcttaa aagcagaaat ttatctgttc ccccctttaa
    79321 aaattcggtg aataccctta cttattggta tactgaagtt gcttcagata aaaacgcatt
    79381 gaacctcgaa taacgttgtc tagtaacacg aattaggggg ccatgatgga aaaaaataat
    79441 gaagtcattc agactcatcc gctcgtaggg tgggacatca gcaccgttga tagctatgat
    79501 gcgctgatgt tgcgtttgca ctaccagacc ccaaataagt ccgagcagga agggactgaa
    79561 gttggtcaga cgctctggtt aaccactgat gttgccagac aatttatttc gatattagaa
    79621 gcaggaatcg ccaaaattga atccggtgat ttcccggtaa acgagtatcg gcgtcattaa
    79681 catgacatga ccatccaccc aaaaggcacc tcaccggtgc ctttaccatt tctgggttgt
    79741 atatctgcat tcagttaatt actctgctta aaccatgaga gcagagagaa aaaaatgaaa
    79801 tacgatctca tcattattgg cagcggttcc gtaggcgctg ccgccgggta ttatgcaacc
    79861 cgcgccggtt taaacgtgct aatgaccgac gcccatatgc caccgcatca acacggcagc
    79921 caccacggcg atacgcgatt aattcgccat gcttatggtg aaggcgaaaa gtatgtcccg
    79981 ttagtcctcc gcgcgcaaac gctgtgggat gaactttccc gccacaacga agatgatccc
    80041 atttttgtac gctctggtgt cattaacctc ggcccggctg actccgcatt tctcgccaac
    80101 gtcgcccaca gcgccgaaca gtggcaactc aacgttgaaa agctcgatgc gcaagggatt
    80161 atggcccgct ggccagaaat acgcgtcccg gacaactaca tcggcttatt tgagactgat
    80221 tccggttttt tgcgcagcga actggcgatt aaaacctgga tccaactggc gaaggaagcg
    80281 ggctgtgcgc aactgttcaa ctgcccggtc accgcaattc gtcatgacga tgatggcgta
    80341 actattgaaa cggttgacgg tgagtatcag gcgaaaaaag cgattgtctg cgcgggaaca
    80401 tgggtaaaag acctgctccc ggagctgcct gtccagcccg tacgtaaagt atttgcctgg
    80461 tatcaggccg atggccgcta tagcgtgaag aataaattcc cggcgtttac cggtgaactg
    80521 cccaatggcg atcaatatta tggttttccg gcagaaaacg acgcgttgaa gattggcaaa
    80581 cataacggag gccaggttat ccattcagcg gatgaacgtg ttccgtttgc ggaagtggcc
    80641 agtgatgggt cagaagcctt cccgttcttg cgcaatgtat tgccgggtat cggttgctgc
    80701 ctgtacggcg ctgcctgcac ctatgataat tcgcctgacg aagattttat tatcgatacc
    80761 ctacccggcc acgataatac actgctcatt accggcctga gtgggcacgg ttttaaattt
    80821 gcgtcagttt taggggaaat agctgccgat tttgcgcaag accaaaaaag cgattttgat
    80881 ttgacgccat tcaggctttc ccgcttccaa taatcatcat tacggcctcc agaatgaggc
    80941 cgtttttaca ggagtattta atgcgtcggc tgttgcacta tctcatcaat aatattcgcg
    81001 agcatctgat gctttatctt tttctctggg gattgttggc aattatggat ctgatctatg
    81061 tgttttattt ttagttttcc gcactgacat aaactgacaa atatttaatc tccctttcat
    81121 ttagcgtgaa aaattgaatg agattattcc tgcttctaca gcactcacca gacatctctg
    81181 atgctgaacc cactaaactt atgaagattc tttcattatt aattcaaaat tattgaaata
    81241 aaaataaata tcattcaatt tgttattttt gtgttgcaat tcattctccg acacgccatt
    81301 ttctttctct cgcaaattcg tattggatgc acttatgtca ttcacaaata cccctgaacg
    81361 ctatggtgtt atatctgcgg ccttccattg gctaagcgca attattgtct acggcatgtt
    81421 tgctttaggt ctgtggatgg tcacgctcag ttattacgat ggctggtatc acaaagcacc
    81481 cgaactgcat aaaagcatcg gtattttgtt aatgatgggg ctggttattc gcgttctgtg
    81541 gcgtgtcata tctccaccgc cgggtccgct gccgagttat tcgcccatga ctcgtcttgc
    81601 cgcaaaagct ggacatctcg cattgtacct attgctgttc gccatcggca tcagcggcta
    81661 tctgatctca actgccgatg gtaaaccgat cagcgttttt ggctggtttg acgtccccgc
    81721 gacccttgcc gacgctggcg cacaggcaga ctttgccggt gccctgcatt tttggctcgc
    81781 gtggagcgtc gtagtactgt ctgttatgca cggatttatg gccctgaagc atcatttcat
    81841 cgataaagac gacactctga agcgcatgct gggaaagtcg tcatctgact atggagtata
    81901 aaaaatgaaa aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc
    81961 agcggttgcc gccgattaca aaattgacaa agaaggtcag cacgcctttg ttaacttccg
    82021 catacagcat cttggctata gctggttata tggcaccttt aaagatttcg acggtacttt
    82081 tacctttgat gaaaaaaatc cggctgccga taaagtgaat gtgacaatta acaccaccag
    82141 cgtcgatact aaccacgccg aacgcgataa acatcttcgc agtgcggatt ttctcaatac
    82201 cacaaaatat ccacaggcaa cattcacctc caccagcgtg aagaaagacg gtgacgaact
    82261 ggatattacc ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc tggaagcgaa
    82321 attaattggt cagggcgacg acccatgggg cggtaaacgt gcaggctttg aggccgaagg
    82381 caaaattaag ctcaaagact tcaatatcaa gacagattta ggtccagctt ctcaggaagt
    82441 ggatctgatt atttcagtgg aaggcgtaca gcagaagtaa gagtgaaatg ttgccggatg
    82501 gcacgcatcc ggcaatgata tttattctgt tggatcagga atacccagtg ttgtattcag
    82561 acgtccacga gacttattaa agatcttatt gccattttca cgtcctgccc gacggcgtcg
    82621 ctgttcttct ggcggtaacg cgctttcttc gcagcaaatc tcactacaac aacctttgta
    82681 tttttccgcg catactggac actgaataaa cagcaggtgg cagccatcat ttttacagtt
    82741 ggtatggctg tcgcacggcg cgccgcactg gtggcaatgc gcgataatct catccgatat
    82801 acgttcgccc atccgctcgt caaaaacaaa atttttgcca ataaaacgca ccggcaagcc
    82861 ctgctcgcgc gccttacggg cgtattcaat aattccgccc tcgatatgcc agactttgtt
    82921 gaatccgtta tgtttcatcc aggcactggc cttttcacaa cgaatgccgc cggtgcagta
    82981 catgacgatt tttttatctt tatgtgcctg catcatctcg actgcttttg gcagctgctc
    83041 acggaaggta tctgccggaa tttcgagcgc gttttcaaag tgccccactt catactcata
    83101 gtggttacgc atgtcgataa atagtgcatc gggatcgtca agcatggcgt tcacttccgc
    83161 cgcttgcaga tactcaccaa cattgctggc atcaaagtga ggatcgtcaa taccgtcggc
    83221 aacaatgcga tcgcgtacct tcatgcgcag tacccagaag gatttcccgt catcatccaa
    83281 cgcgatattc aggcgtaaac cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa
    83341 tgtttcaaca ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat gcgccagata
    83401 cactcgccca aaaacattca gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt
    83461 aggatcggcg atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt cgctctcagc
    83521 caacattttg gcttttaacg cgtcgttgga aatgcggttg tgtaacactg gcatggtgta
    83581 cggttcctgc gagatgggaa agtaaaaatc cgcggcatga tatagcaatt atcgataatt
    83641 aacatccaca cattttacgc tacatttgcg cattaaaaat tatttgttat ttacaagcgc
    83701 ggcaatttcg cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata
    83761 catacaattg cccgtatagg ttgaaaaaca ggattgatat gacgaatcta cccaagttct
    83821 ccaccgcact gcttcatccg cgttattggt taacctggtt gggtattggc gtactttggt
    83881 tggtcgtgca attgccctac ccgattatct accgcctcgg ttgtggatta ggaaaactgg
    83941 cgttacgttt tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc
    84001 cggaaatgag cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca
    84061 tgggcctgat ggaaaccggt atggcgtggt tctggccgga ccgccgaatc gcccgctgga
    84121 cggaagtgat cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt
    84181 tagttggcat ccattttctg acactggagc tgggcgcgcg gcagtttggt atgcaggaac
    84241 cggggattgg cgtttaccgc ccgaacgata atccactgat tgactggcta caaacctggg
    84301 gccgtttgcg ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc atgattaaag
    84361 ccctgaaaaa aggcgaagtg gtctggtacg caccagatca tgattacggc ccgcgctcaa
    84421 gcgttttcgt cccgttattt gccgttgagc aggctgcgac cacgaccgga acctggatgc
    84481 tggcacggat gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag ccagatggca
    84541 aagggtatca attgattatg ctgccgccag agtgttctcc gccactggat gatgccgaaa
    84601 ctaccgccgc gtggatgaac aaagtggtcg aaaaatgcat catgatggca ccagagcagt
    84661 atatgtggtt acaccgtcgc tttaaaacac gcccggaagg cgttccttca cgctattaaa
    84721 tctcccatgc cggatgcttc agaatggcat ccggcattac cacagcaaat ccccctgatt
    84781 tagcgataaa agctctctgg attgcgcccc ctggaagtcg ggcgcataat tagtgtgttt
    84841 atcttttctt cttatgttca ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct
    84901 gtgaaaatga cacccctata aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt
    84961 ttcttaccgg ggccgccttc agtctggtaa tgcccttctt acccctctac gttgagcagc
    85021 ttggtgttac cggccactcc gccctgaata tgtggtccgg tattgtcttc agtataatgg
    85081 gttcctaaac acatcatttt agatattgaa tatgagacgg gcaatcagct acatcagatt
    85141 ttcaagtgaa cgacaactga aaggggactc tgtacgccga caatctaagc tggttactga
    85201 ctggcttgat aagaaccctg agttttactt ggattcatcc ttgagcttta aagatttggg
    85261 gaaaagtgct tttagtggta agcacctgaa aggtggtcta ggtgactttt taaccgctat
    85321 cgagaaaggg cttgttaaag ctggtgatac gctactaatc gagagtttag accgtctgtc
    85381 acgtcaggat attgatattg cttctgagct attacgccgt atcttaaggg ctggtgtaga
    85441 tgtagtcaca ctgagcgatg gagaacatta cacacgagaa agtttaaaag acccgttagc
    85501 cctgattaaa agcattctga taatgcagcg ggcgcatgag gaaagtttga ggaagtctga
    85561 gcgtgtccaa gctgcatgga accgtaaaaa agaactcatc tccgaaggta tcaaggtatc
    85621 taggcgttgt cctgcatggt taaggctgaa tgatgaccgt cgaacattca caatcattcc
    85681 tgataaagtg gaagttgtta aacgagcatt tgacctgaga ttacagggtt taagcttttg
    85741 ggctatcacc agaactttaa acgatgaagg tcacttgtca ttgaatcaat acacacccaa
    85801 acaaaagggg tggtctgata ctgctgtaaa gaaactactc agaaatcgtg ctgtgattgg
    85861 atgtttcaca cctgctggac gtgaagaagt tcagggttac taccccgcaa taatcagtga
    85921 gtcactattc tatcgagtac agcaactgaa cacagggcaa tatggtaggg cttctgtatc
    85981 aagtaatccg ctatcagtca acttgttcag gggtatcatc aaatgttctg agtgtggggc
    86041 tactattgtt cttggtggat atgccttaaa acgttttgga atgtacagat gccctaatcg
    86101 ttcagctaac aggtgtagtg caaaggctat cagtcgtaag caaacggatg caactatcct
    86161 ttacatgctc gcattatgcg accgttttga aactgaaaat actgacacta ttgatagcct
    86221 taaattacag cgtgaagatt tg
//
LOCUS       DAJTNM010000004        82579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTNM010000004 DAJTNM010000000
VERSION     DAJTNM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.004"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..442)
                     /locus_tag="OO172_000338"
     CDS             complement(<1..442)
                     /locus_tag="OO172_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR5510645.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELL"
     gene            complement(513..2627)
                     /gene="fusA"
                     /locus_tag="OO172_000339"
     CDS             complement(513..2627)
                     /gene="fusA"
                     /locus_tag="OO172_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5510646.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2724..3194)
                     /gene="rpsG"
                     /locus_tag="OO172_000340"
     CDS             complement(2724..3194)
                     /gene="rpsG"
                     /locus_tag="OO172_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5510647.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3291..3665)
                     /gene="rpsL"
                     /locus_tag="OO172_000341"
     CDS             complement(3291..3665)
                     /gene="rpsL"
                     /locus_tag="OO172_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5510648.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3791..4078)
                     /gene="tusB"
                     /locus_tag="OO172_000342"
     CDS             complement(3791..4078)
                     /gene="tusB"
                     /locus_tag="OO172_000342"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5510649.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4086..4445)
                     /gene="tusC"
                     /locus_tag="OO172_000343"
     CDS             complement(4086..4445)
                     /gene="tusC"
                     /locus_tag="OO172_000343"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5510650.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4445..4831)
                     /gene="tusD"
                     /locus_tag="OO172_000344"
     CDS             complement(4445..4831)
                     /gene="tusD"
                     /locus_tag="OO172_000344"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5510651.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4831..5553)
                     /locus_tag="OO172_000345"
     CDS             complement(4831..5553)
                     /locus_tag="OO172_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5510652.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5720..6532)
                     /gene="fkpA"
                     /locus_tag="OO172_000346"
     CDS             complement(5720..6532)
                     /gene="fkpA"
                     /locus_tag="OO172_000346"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5510653.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6753..6971
                     /locus_tag="OO172_000347"
     CDS             6753..6971
                     /locus_tag="OO172_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5510654.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7020..7610)
                     /gene="slyD"
                     /locus_tag="OO172_000348"
     CDS             complement(7020..7610)
                     /gene="slyD"
                     /locus_tag="OO172_000348"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5510655.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETEQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7705..7905)
                     /locus_tag="OO172_000349"
     CDS             complement(7705..7905)
                     /locus_tag="OO172_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5510656.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7915..9720)
                     /gene="kefB"
                     /locus_tag="OO172_000350"
     CDS             complement(7915..9720)
                     /gene="kefB"
                     /locus_tag="OO172_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR5510657.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9720..10271)
                     /gene="kefG"
                     /locus_tag="OO172_000351"
     CDS             complement(9720..10271)
                     /gene="kefG"
                     /locus_tag="OO172_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5510658.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10402..12315
                     /locus_tag="OO172_000352"
     CDS             10402..12315
                     /locus_tag="OO172_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5510659.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12315..13337
                     /locus_tag="OO172_000353"
     CDS             12315..13337
                     /locus_tag="OO172_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5510660.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13331..13549
                     /locus_tag="OO172_000354"
     CDS             13331..13549
                     /locus_tag="OO172_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5510661.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13603..14472
                     /locus_tag="OO172_000355"
     CDS             13603..14472
                     /locus_tag="OO172_000355"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5510662.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14527..14931)
                     /locus_tag="OO172_000356"
     CDS             complement(14527..14931)
                     /locus_tag="OO172_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5510663.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15233..15865
                     /gene="crp"
                     /locus_tag="OO172_000357"
     CDS             15233..15865
                     /gene="crp"
                     /locus_tag="OO172_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5510664.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15916..18006
                     /locus_tag="OO172_000358"
     CDS             15916..18006
                     /locus_tag="OO172_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5510665.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18073..19293)
                     /gene="argD/dapC"
                     /locus_tag="OO172_000359"
     CDS             complement(18073..19293)
                     /gene="argD/dapC"
                     /locus_tag="OO172_000359"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5510666.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19379..19942)
                     /gene="pabA"
                     /locus_tag="OO172_000360"
     CDS             complement(19379..19942)
                     /gene="pabA"
                     /locus_tag="OO172_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5510667.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19974..20576)
                     /locus_tag="OO172_000361"
     CDS             complement(19974..20576)
                     /locus_tag="OO172_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5510668.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20566..20733)
                     /locus_tag="OO172_000362"
     CDS             complement(20566..20733)
                     /locus_tag="OO172_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5510669.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20838..21410)
                     /gene="ppiA"
                     /locus_tag="OO172_000363"
                     /gene_synonym="rotA"
     CDS             complement(20838..21410)
                     /gene="ppiA"
                     /locus_tag="OO172_000363"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5510670.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21681..22862
                     /gene="tsgA"
                     /locus_tag="OO172_000364"
     CDS             21681..22862
                     /gene="tsgA"
                     /locus_tag="OO172_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5510671.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23124..25667
                     /gene="nirB"
                     /locus_tag="OO172_000365"
     CDS             23124..25667
                     /gene="nirB"
                     /locus_tag="OO172_000365"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5510672.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKGDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25664..25990
                     /gene="nirD"
                     /locus_tag="OO172_000366"
     CDS             25664..25990
                     /gene="nirD"
                     /locus_tag="OO172_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5510673.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26116..26922
                     /gene="nirC"
                     /locus_tag="OO172_000367"
     CDS             26116..26922
                     /gene="nirC"
                     /locus_tag="OO172_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5510674.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26941..28314
                     /gene="cobA"
                     /locus_tag="OO172_000368"
     CDS             26941..28314
                     /gene="cobA"
                     /locus_tag="OO172_000368"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5510675.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28561..28728
                     /locus_tag="OO172_000369"
     CDS             28561..28728
                     /locus_tag="OO172_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5510676.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            29023..30360
                     /gene="frlA"
                     /locus_tag="OO172_000370"
                     /pseudo
     CDS             29023..30360
                     /gene="frlA"
                     /locus_tag="OO172_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30381..31403
                     /gene="frlB"
                     /locus_tag="OO172_000371"
     CDS             30381..31403
                     /gene="frlB"
                     /locus_tag="OO172_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5510677.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITLLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31453..32283
                     /gene="frlC"
                     /locus_tag="OO172_000372"
     CDS             31453..32283
                     /gene="frlC"
                     /locus_tag="OO172_000372"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5510678.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIYMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32280..33065
                     /gene="frlD"
                     /locus_tag="OO172_000373"
     CDS             32280..33065
                     /gene="frlD"
                     /locus_tag="OO172_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5510679.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33165..33896
                     /gene="phoB"
                     /locus_tag="OO172_000374"
     CDS             33165..33896
                     /gene="phoB"
                     /locus_tag="OO172_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5510680.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34056..35141)
                     /locus_tag="OO172_000375"
     CDS             complement(34056..35141)
                     /locus_tag="OO172_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5510681.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35153..36457)
                     /locus_tag="OO172_000376"
     CDS             complement(35153..36457)
                     /locus_tag="OO172_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5510682.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEMALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36469..36760)
                     /locus_tag="OO172_000377"
                     /pseudo
     CDS             complement(36469..36760)
                     /locus_tag="OO172_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 family protein"
     gene            complement(36771..37649)
                     /locus_tag="OO172_000378"
     CDS             complement(36771..37649)
                     /locus_tag="OO172_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5510683.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37646..38872)
                     /locus_tag="OO172_000379"
     CDS             complement(37646..38872)
                     /locus_tag="OO172_000379"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5510684.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38873..40036)
                     /locus_tag="OO172_000380"
     CDS             complement(38873..40036)
                     /locus_tag="OO172_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5510685.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(40120..40482)
                     /locus_tag="OO172_000381"
     CDS             complement(40120..40482)
                     /locus_tag="OO172_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5510686.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40499..41404)
                     /locus_tag="OO172_000382"
     CDS             complement(40499..41404)
                     /locus_tag="OO172_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510687.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41674..42678)
                     /gene="trpS"
                     /locus_tag="OO172_000383"
     CDS             complement(41674..42678)
                     /gene="trpS"
                     /locus_tag="OO172_000383"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5510688.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42671..43429)
                     /gene="gph"
                     /locus_tag="OO172_000384"
     CDS             complement(42671..43429)
                     /gene="gph"
                     /locus_tag="OO172_000384"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5510689.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43422..44099)
                     /gene="rpe"
                     /locus_tag="OO172_000385"
     CDS             complement(43422..44099)
                     /gene="rpe"
                     /locus_tag="OO172_000385"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5510690.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44117..44953)
                     /gene="dam"
                     /locus_tag="OO172_000386"
     CDS             complement(44117..44953)
                     /gene="dam"
                     /locus_tag="OO172_000386"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5510691.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45060..46346)
                     /gene="damX"
                     /locus_tag="OO172_000387"
     CDS             complement(45060..46346)
                     /gene="damX"
                     /locus_tag="OO172_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5510692.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46438..47526)
                     /gene="aroB"
                     /locus_tag="OO172_000388"
     CDS             complement(46438..47526)
                     /gene="aroB"
                     /locus_tag="OO172_000388"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5510693.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47583..48104)
                     /gene="aroK"
                     /locus_tag="OO172_000389"
     CDS             complement(47583..48104)
                     /gene="aroK"
                     /locus_tag="OO172_000389"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5510694.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48505..49743)
                     /gene="hofQ"
                     /locus_tag="OO172_000390"
     CDS             complement(48505..49743)
                     /gene="hofQ"
                     /locus_tag="OO172_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5510695.1"
                     /translation="MKQWIAALLLMLISGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49655..50059)
                     /gene="hofP"
                     /locus_tag="OO172_000391"
     CDS             complement(49655..50059)
                     /gene="hofP"
                     /locus_tag="OO172_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR5510696.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADIRRTGGKAAKSDADGG"
     gene            complement(50049..50489)
                     /gene="hofO"
                     /locus_tag="OO172_000392"
     CDS             complement(50049..50489)
                     /gene="hofO"
                     /locus_tag="OO172_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5510697.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50473..51012)
                     /gene="hofN"
                     /locus_tag="OO172_000393"
     CDS             complement(50473..51012)
                     /gene="hofN"
                     /locus_tag="OO172_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5510698.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(51012..51791)
                     /gene="hofM"
                     /locus_tag="OO172_000394"
     CDS             complement(51012..51791)
                     /gene="hofM"
                     /locus_tag="OO172_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5510699.1"
                     /translation="MTFKIWQIGLHLQQQEVAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51911..54463
                     /gene="mrcA"
                     /locus_tag="OO172_000395"
     CDS             51911..54463
                     /gene="mrcA"
                     /locus_tag="OO172_000395"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5510700.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54628..55188)
                     /gene="nudE"
                     /locus_tag="OO172_000396"
     CDS             complement(54628..55188)
                     /gene="nudE"
                     /locus_tag="OO172_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5510701.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55508..57643
                     /gene="igaA"
                     /locus_tag="OO172_000397"
     CDS             55508..57643
                     /gene="igaA"
                     /locus_tag="OO172_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5510702.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57708..58376
                     /gene="yrfG"
                     /locus_tag="OO172_000398"
     CDS             57708..58376
                     /gene="yrfG"
                     /locus_tag="OO172_000398"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5510703.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58387..58788
                     /gene="hslR"
                     /locus_tag="OO172_000399"
     CDS             58387..58788
                     /gene="hslR"
                     /locus_tag="OO172_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5510704.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58813..59691
                     /gene="hslO"
                     /locus_tag="OO172_000400"
     CDS             58813..59691
                     /gene="hslO"
                     /locus_tag="OO172_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5510705.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59826..61550)
                     /locus_tag="OO172_000401"
     CDS             complement(59826..61550)
                     /locus_tag="OO172_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5510706.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61929..63551
                     /gene="pckA"
                     /locus_tag="OO172_000402"
     CDS             61929..63551
                     /gene="pckA"
                     /locus_tag="OO172_000402"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5510707.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63627..64979)
                     /gene="envZ"
                     /locus_tag="OO172_000403"
     CDS             complement(63627..64979)
                     /gene="envZ"
                     /locus_tag="OO172_000403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5510708.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64976..65695)
                     /gene="ompR"
                     /locus_tag="OO172_000404"
     CDS             complement(64976..65695)
                     /gene="ompR"
                     /locus_tag="OO172_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5510709.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65923..66399
                     /gene="greB"
                     /locus_tag="OO172_000405"
     CDS             65923..66399
                     /gene="greB"
                     /locus_tag="OO172_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5510710.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66496..68817
                     /locus_tag="OO172_000406"
     CDS             66496..68817
                     /locus_tag="OO172_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5510711.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69255..69482
                     /gene="feoA"
                     /locus_tag="OO172_000407"
     CDS             69255..69482
                     /gene="feoA"
                     /locus_tag="OO172_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5510712.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69499..71820
                     /gene="feoB"
                     /locus_tag="OO172_000408"
     CDS             69499..71820
                     /gene="feoB"
                     /locus_tag="OO172_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5510713.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGMGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71820..72056
                     /gene="feoC"
                     /locus_tag="OO172_000409"
     CDS             71820..72056
                     /gene="feoC"
                     /locus_tag="OO172_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5510714.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72259..73137
                     /gene="rpnA"
                     /locus_tag="OO172_000410"
     CDS             72259..73137
                     /gene="rpnA"
                     /locus_tag="OO172_000410"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5510715.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            73210..73479
                     /locus_tag="OO172_000411"
     CDS             73210..73479
                     /locus_tag="OO172_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5510716.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73473..73838
                     /locus_tag="OO172_000412"
     CDS             73473..73838
                     /locus_tag="OO172_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5510717.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73839..74627)
                     /gene="bioH"
                     /locus_tag="OO172_000413"
     CDS             complement(73839..74627)
                     /gene="bioH"
                     /locus_tag="OO172_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5510718.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74665..75348
                     /gene="gntX"
                     /locus_tag="OO172_000414"
     CDS             74665..75348
                     /gene="gntX"
                     /locus_tag="OO172_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5510719.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75407..75982
                     /gene="nfuA"
                     /locus_tag="OO172_000415"
     CDS             75407..75982
                     /gene="nfuA"
                     /locus_tag="OO172_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5510720.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76343..77647
                     /gene="gntT"
                     /locus_tag="OO172_000416"
     CDS             76343..77647
                     /gene="gntT"
                     /locus_tag="OO172_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5510721.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77692..79776)
                     /gene="malQ"
                     /locus_tag="OO172_000417"
     CDS             complement(77692..79776)
                     /gene="malQ"
                     /locus_tag="OO172_000417"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5510722.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTTTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79786..82179)
                     /gene="malP"
                     /locus_tag="OO172_000418"
     CDS             complement(79786..82179)
                     /gene="malP"
                     /locus_tag="OO172_000418"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5510723.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19803 a        22430 c        20837 g        19509 t
ORIGIN      
        1 ccagcagctc ttcgtcatca accatgtcgc atttgttcag gaacacgatg atgtacggaa
       61 cgcctacctg acgacccagc aggatgtgct cacgagtctg cggcatcggg ccgtcagtcg
      121 cagcaactac caggatcgcg ccgtccatct gagcagcacc ggtgatcatg tttttaacat
      181 agtcggcgtg ccccgggcag tctacgtgtg cgtagtgacg ggtcggggtg tcatattcaa
      241 cgtgagaagt gttgatggtg ataccacgag ctttttcttc cggcgcgtta tcgatctggt
      301 cgaatgcacg agcagcaccg ccgtaggttt tagccagtac ggtggtgatt gcagcggtca
      361 gagtagtttt accgtggtca acgtggccga tagtaccaac gttaacgtgc ggttttgtac
      421 gttcaaattt ttctttagac acggctatat tccttactat agtgctctcc ccttcaggag
      481 agagcacggg actttggtat taacccttag gcttatttac cacgggcttc aattacggcc
      541 tgagcaacgt tactcggcgc ttcatcatac ttcaggaatt ccatagtgta tgatgcacga
      601 cctttggtca gagaacgcag ctgagttgcg tatccgaaca tttcagacag cggtacttca
      661 gcgtggatct taacgccagt aacttcagat tcctgacctt tgagcatacc acgacgacgg
      721 ctcaagtcac cgataacgtc accggtgttc tcttccggag tttctacttc aaccttcatg
      781 atcggctcaa gcagaactgg tttcgctttc ttaaagcctt ctttaaaggc gatagaagca
      841 gccagtttaa acgccagttc agaggagtca acgtcatggt aagaaccgaa gtgcagacga
      901 atacccatgt ctactaccgg gtagcctgcc agcggacctg ctttcagctg ttcctggata
      961 cctttatcaa cggccgggat gtattcgcca gggattacac cacctttaat gtcgttgatg
     1021 aactcgtagc ctttcgggtt tgaacccggc tccagcgggt acatgtcgat aacaacatga
     1081 ccatactgac cacgaccacc agactgtttc gcgtgtttac cttcaacatc ggtaactttc
     1141 tggcggatag tttcacggta agcaacctgc ggtttaccta cgttcgcttc aacgttgaat
     1201 tcacgcttca tacggtcaac gatgatgtcg aggtgcagtt cgcccatacc cgcgatgatg
     1261 gtctggttag attcttcgtc agtccataca cggaaagacg ggtcttcttt agccagacgg
     1321 cccagagcca gacccatttt ttcctggtca gctttggttt tcggttcaac tgcgatggag
     1381 attaccggct cagggaattc catacgttcc agaatgatcg gcgcatccgg gtcacacagg
     1441 gtgtcaccag tggttacgtc tttcagaccg atagcagcag cgatgtcgcc cgcgcgaact
     1501 tctttgatct cttcacgttt gttagcgtgc atctgaacga tacgaccgaa acgctcacgt
     1561 gcagctttca cggagttcag tacggtatca ccagagttaa ccacaccgga gtaaacacgg
     1621 aagaaggtca ggttaccaac aaacgggtcg gtagcgattt tgaacgccag tgcagagaac
     1681 ggctcgtcat cacttgcgtg acgttcagcc ggagtgtctt taccgtcgtc caggataccg
     1741 ttgatcgcag gtacgtcaac cggggatggc aggtaatcaa ttaccgcatc cagcatcgcc
     1801 tgaacacctt tgttcttgaa cgcagaacca caggttacca ggatgatttc gttgttcaga
     1861 acgcgctgac gcagagcacc tttgatttct gcttcagtca gttcttcacc acccaggtat
     1921 ttttccatca gctcttcaga agcttcagct gcggattcga tcaggttctg gtgccattcg
     1981 ttagccagtt caaccatgtc tgccgggata tcttcgtatt cgaaggttac gccctggtca
     2041 gcgtcgttcc agttgatagc tttcattttc accaggtcaa caacaccggt gaaatgttct
     2101 tcagcaccaa tcgccagctg cagcggaacc gggttcgcgc ccagacgggt tttgatctgg
     2161 ttaacaactt tcaggaagtt cgcacccatg cggtccattt tgttaacgaa cgcaatgcgc
     2221 ggaactttat atttgtttgc ctgacgccat acggtttcag actgcggctg aacaccacca
     2281 actgcgcagt aaaccattac cgcaccatcg agaacacgca tggaacgttc tacttcgatt
     2341 gtgaagtcaa cgtgccccgg ggtgtcgatg atgttgatgc gatgcggctc atactgctta
     2401 gccataccag accagaatgc agtagtcgca gcggaagtga tggtaatacc acgttcctgc
     2461 tcctgctcca tccagtccat ggttgcagcg ccgtcatgaa cttcaccgat tttatggttt
     2521 acaccggtgt agaacagaat acgttcggta gtagtggttt taccggcgtc gatgtgcgca
     2581 ctgataccga tgttacggta gcgtgcgatg ggtgttgtac gagccatttg tttcctcgtt
     2641 tatcttttag gcgttcaatt taagtagccc aaagcgggct gcttactgga agcgcccgcc
     2701 tggtgactaa aactccgaag ggattaccaa cggtagtgtg cgaacgcctt gttggcttcg
     2761 gccatacggt gaacgtcttc acgtttctta actgcagtac ctttgttttc tgcagcatca
     2821 gaaagttcgt tcgccaggcg cagagccatg gatttatcac cgcgtttacg agcagcttca
     2881 acgatccaac gcattgccag agcattacga cgaaccggac ggacttcaac tggtacctga
     2941 taagtagaac caccaacgcg gcgagactta acttctacag tcgggcgcac gttttcgaga
     3001 gctacttcga atgcttccag ttcagattta ccagagcgct gagccagggt ctccagcgcg
     3061 ctgtatacga tagattcagc agtagatttt ttaccatcta ccatcaggat atttacaaat
     3121 ttagccagca gttctgatcc gaacttcgga tccggcagaa ttttacgctg accaatgacg
     3181 cgacgacgtg gcatggaaat actccgttgt taattcagga ttgtccaaaa ctctacgagt
     3241 ttagtttgac atttaagtta aaacgtttgg ccttacttaa cggagaacca ttaagcctta
     3301 ggacgcttca cgccatactt ggaacgagcc tgcttacggt ctttaacgcc ggagcagtca
     3361 agcgcaccac gtacggtgtg gtaacgaaca cccgggaggt ctttaacacg accgccacgg
     3421 atcaggatca cggagtgctc ctgcaggttg tgaccttcac caccgatgta ggaagtcact
     3481 tcgaaaccgt tagtcagacg aacacggcat actttacgca gcgcggagtt cggtttttta
     3541 ggagtggtag tatatacacg agtacatacg ccacgttttt gcgggcatgc ttccagcgca
     3601 ggcacgttgc ttttcgcaac tttgcgagca cgtggtttgc gtaccagctg gttaactgtt
     3661 gccattaaat agctcctggt tttagctttt gcttcgtaaa cacgtaataa aacgtcctca
     3721 cacaatatga ggacgccgaa ttttagggcg atgccgaaaa ggtgtcaaga aatatacaac
     3781 gatcccgcca tcaccaggcc atctggctgg agtgcttaac cgtaagtctg acgaaatcag
     3841 tatagtcaat gggaatgatg tcgttcaaaa tttgaacagt caaaccgcgg gcaataaggt
     3901 cttcgttcag ggcatagacc ttaatggggg cattacgcag actttcaagg tagcggttac
     3961 cgtcaactgc ggcagttacg ccatcttgca ataatagcag ttcgtctcct tcactgagca
     4021 gacgcagcag cgcagcaaaa tccgtcagcc agggtgaacg atgtaacgtg tgcagcataa
     4081 acgcctcaaa acctcaaaat aacatcgtag ttggcgagtt cgcggcgtaa ggcatctgct
     4141 tcgagcggcg tggcttcgac aacaaagggt gtctgcggat ctaacccgcg ttcgcgcagt
     4201 gaagccgcac aaacccagca ctgttcaatg tcgtacagac tcaacaattt aaaagtggca
     4261 atgtaatcac gcgccagcac tgcatcgggc ttttgtcctg acagcagctg aaaaacgcca
     4321 tcagcaataa agaagacggc cagatcgtca gttaatgcgg aagttgccag taaagcatct
     4381 aaaccttccc gaccagcagc tgtaccatga ggtgcagtag aaaaaacaaa cgcaattcgt
     4441 ttcatcagaa ctgtaccacc ctgtcacagg tcagcgaggc ttccgccagc gcgccaagtc
     4501 cgcttaaggt aaatcccggc tgaaggtttg acgaagccag ccccagcctt cctgcttcag
     4561 tttcatcgac gataccgcgg cgtaacgctg ccgctacgca gatattcagc gccacaccat
     4621 gttgcgcatt caactgttgc cagccgcgca caaggtcaaa ttcgtcgctt gccggtgagg
     4681 tcagttggtt agcgttatag accccttccc gatagaaaaa gacgctgctt aacttatggc
     4741 cttctgctat cagcgcctgc gcaaactgaa aagcactact cgcctgttgc gtaccgtatg
     4801 ctggcccggt caccacgatg gcaaaacgca ttacttatct tgcccctgaa aatcaccgct
     4861 cttgaactgg cggatgtaga gatagacagt gtgtttggag atgttcaggc ggtcagcaac
     4921 ctggttgatc gcatctttaa tatcgaagat ccctttctcg tagagattca gcacgatctg
     4981 acgatttttg gcgttattag aaacattgcg atcggcattc acttcttcga tggtgaactc
     5041 cagcgtttgg gtaaccagat cttcaacaga agaggcaaag ttgacgcttg aaccgacatc
     5101 cggggtttct ggcggcacaa aagtgctcat aatctgcgag aagggaacat caagattcat
     5161 attgatgcac agcagaccaa ttacacgctg ttcgcggtta cgaatcgcga tagtcaggga
     5221 cttcattaat acgccgcttt tggcgcgagt aaagtagcat ttagaaacgc tgctatccgc
     5281 tcccgtcata tcgtgcagca tacgtagcgc caggtcagta attggcgaac caatcttccg
     5341 gcctgtatgt tcaccgttag caatgcgaat ggctgaacat tttagatcct gcaaagagtg
     5401 caaaacgatt tcacagtggg agccaataag catcgctaac ccgtccacca ccgcttcgta
     5461 ggatttcaga atatcaaaat cggtctggtc gaaaggacgt tgatccagta aatccaactc
     5521 actggtttcg ttggttaaaa gcgacctgga catgaaaaaa aacactcctt ttcaggagcc
     5581 tgtcgttaac ttttcagggc aggctcatta atgatgcggg taactaaatt aatacagcgg
     5641 aggttccgct ttccagcact aattatatcc ggcctgtaat aaaaaaaccg ccgcctggtc
     5701 aggcggcggt tcttaatgct tattttttag cagaatctgc ggctttcgca tcagcttccg
     5761 gctttgcatc agccttcggc gctggtttca catccagcag ctctacgtca aacactaggg
     5821 tagaattcgg tgggatcccc ggaacacccg ctttgccgta agccagttct ggtggaataa
     5881 ccagtttgat cttaccgcct ttcttgatgt tcttcagacc ttctgtccaa cccgggataa
     5941 caccgtccag acggaaagaa agcggttcac cacgggtgta agagttgtcg aactctttac
     6001 cgtcgatcag cgtacctttg tagttcacta caacagtatc gctgtctttc ggtgcttcgc
     6061 ctttaccggc ttctactacc tgataaacca gaccagttga agaggttttc acacctttct
     6121 ctttggcaaa tttctcgcgg tactctttac cttttgcttc gttatcagcc gcgtcttttt
     6181 ccatcttcgc ctgagcagaa gacttcacgc gagcttcgaa tgcttgcaga gtctgttcga
     6241 tctcttggtc ggagagtttg ctcttatcag caaatgcatc ctgaacacca gcgatcagct
     6301 gatctttatc cagtttgatg cccagttttt cttgttcttt aagagagttt tccatgtaac
     6361 gacccagcga agcacccagt gcataagctg atttctgatc gtcatttttg aacgctgctt
     6421 tgctgtcagc agttgtagca ggttttgcag cttcagcagc aaaagtgatt ggtgcatgca
     6481 gggcaacggc cattgtggtc gccagtagcg ttactttaaa cagtgatttc atccatatct
     6541 ccaggatcgg ggcatctcac cccagggtta actattatca gaagggtact ataaagcgtt
     6601 gtcgaacaaa tctacataca gacacgccct attatcatct attttcagac tctttttgtt
     6661 taaattagtt tcgatgaccg cgaaatgagt gctgtctcgg gcagcaaagt taagtagaat
     6721 ccgcggcgac cattcgacaa aagaggtgaa tcatgcagga tttatcattg gaagcacgcc
     6781 tggcagagct ggagagccga ctggcttttc aggaaatcac cattgaagaa ctgaacgtca
     6841 cggtgaccgc tcatgaaatg gagatggcga aactgcgcga tcatctgcgt ctgctgaccg
     6901 agaagttaaa agccagccag ccgtcgaaca tcgcgtcgca ggctgaagaa acgccaccgc
     6961 cacattattg aggcgtaaaa aaagcgggga ttccccgctt ttttgtcacc ttttcggtat
     7021 tagtggcaac cacaaccgcc gttgccttta ccgccacagc agccttcgcc accgtgttca
     7081 tgaccgtgat cgtggccatg accgccgcag caaccgtcgt ggtcgtgatc gtggtgatga
     7141 tcgtgcgcgc cgtgaacgtg gccatgagcc agttcttctt cggtcgcttc gcgaatcgcc
     7201 acaacttcaa cgttgaattt caggttctga ccggccagca tgtggttacc atcaaccact
     7261 acgtgatcgt cttcaaccgc agtgatttca accggtaccg gaccctgctc agtttcagcc
     7321 aggaaacgca tacctacctg cagttcatca acgcccataa atacgtcttt aggaacacgt
     7381 tgcactaggt tttcgtcgta ctgaccgtaa gcgtcgttcg cgccaacagc gacatcaaat
     7441 ttgtcgccaa cttcatgacc ttccagcgcc gtttccaggc cagagatcag ggaaccgtga
     7501 ccatgcaggt agtccagcgg cgcactcacc ggagactcat caaccaacac accgtcttct
     7561 gtacgtacct gataggccag gctgaccacc aggtcttttg ctactttcat gatatctcct
     7621 gagcatggga agaatagtgg cgcagattgt agcggaattc tgcagccgtg tactcactag
     7681 cttaaaaaaa ctcggcgcat atcgctagtc cggatgaaaa atcccgatca cttgctcatc
     7741 tttgtgaacg tgatcgcggg cttctttgtc tgcttctcgc atctgatgtc cgcacttaac
     7801 acattcaaca atatcaatat tattttcgcg ccacatcgcc attgaatcct gcgcctgaca
     7861 ggccgggcat tttgcgcccg ctataaaacg ttttcggatt gccatcctta ccctctactc
     7921 aaattcatcc cagccgtcca gctggcgtcg ttcttgttgc atttcacgct ggaaaatctc
     7981 ttccagttcg cgtcgggctt ccctggcgcg agaaatttgt acggtatcag catgcatcgg
     8041 gattaactcc cgcagcattc gcatatccag gcggcgaaaa tgcagttgcg cccgctgtgc
     8101 ctgatgcgga tgcatgccaa gcgtgaccag cgtcttgcgc cccagctcta acgcactgga
     8161 gaatgtttca cgggaaaact gcgtcacccc tgcctgtaat aactcatgcg cttccacacg
     8221 tccgcgcact cgcgcaagaa tatgcaaatg cggaaagtgc tgctggcata tttccaccag
     8281 cttcatggtg tcttccggct cgttacaggt aatgacgata gactcagcgg cctctgcacc
     8341 cgcagaacgt aaaagatcga cctgcgtggc gtcgccgtaa taaactttgt agccatattt
     8401 acgcatcagg ttaacggcgc tgatatcccg ctccagcacg gtaatgcgca ttttatttgc
     8461 catcagcaaa cgaccaatca cctgaccaaa acgcccgaag cccacgacaa tgacctgggg
     8521 tttatcatcg ttgacccacg gtttttcgtc ttcttcttcc ggtccgttaa actggcggga
     8581 tagccattta tccaccagct tcatcagcaa cggcgtggtc atcatggaaa gcgtcaccgt
     8641 caccagcaac agcgccatct ggtcgccctg gaataaacgt tgtgaagaag cggtagaaaa
     8701 gaggacaaag gcaaactcac cgccctgact caacacgcca gcaaactgca tccgctctga
     8761 gctacgcacg ccatacaatc gcgccagcag atacagcacg agaattttca ccgccaccag
     8821 cacaaccacg cttatcacta cccacaacag atgggtataa agcaccccga ggttgagcga
     8881 catgccgaca gagataaaga acaaaccgag cagcaagcct ttgaagggat cgatagccgt
     8941 ttccagttca tggcgatatt cactttccgc cagcagcacg cccgcaataa atgtaccgag
     9001 cgccatcgac agccccagcg catccataaa caatgcggaa cccaacacca gcagcagcgt
     9061 cgcggcggtg aacacttccc gcacgccaga agctgcaata aagcggaata ccggacgcag
     9121 taaatagcgc ccaccaatca gcatgccgac aaacgccagc accttcatgc cgatcttcat
     9181 ccagtcgaaa tgttcgtctg ccgaccccgc caacaacggc actaacgcca gtgctgggat
     9241 cactgccaga tcctgaaaca gcagaaccga aaacccgagc tggccggatt cgctgcgatt
     9301 catccctttc tcacgcatca attgcaacgc cattgcagtt gaagacatcg caaggccaat
     9361 gccaccgacc accgccgcct gccaggcgaa atccgtcagc atcaataatc ccgccagcaa
     9421 cgccgcgctt aacagcacct gtgccacgcc tacgccaaaa atcgaacgcc gcagttgcca
     9481 aagtttggag ggattcaact caaggccgat gataaacatc aggaatacca cgccgagttc
     9541 cgaaaagtgg aggatctcat cgacgtcgct aataaacccc agcccccacg ggccaattgc
     9601 aatccctgcc agcaaatatc ccaacacagc gccaatcccc agccgcgatg ccaacggcac
     9661 cgcagccacc gccgcgaaga gaaacagcac tcctgcgagt aaaaaatcgg aaccttccat
     9721 cagcggcctc ctggagacag cggatttgcc aaccagtcac cgtaggctct ggcgtggctc
     9781 gccagctcct ttgcgctttg ccgtctcgcc cagtaaataa tgatggaact taaccaatgc
     9841 atccggcaca tgcccgccgc cagttcaaag gggcgcagca catcgctcat cgggtagcga
     9901 ttcagcgcgt cataacggta agcactttcc ggctcgccgg tggtaatcac gttacgccag
     9961 tactttcccg ccagttggtt tcctcccggc ccgctggcaa aaccacgact taatacccgg
    10021 tccagccact ctttcagtag cgccgggcag ctataggtat aaagaggatg ctgaaagaca
    10081 atcacctcgt gctcgcgcag taatgcctgc tcacggggga tatcaataaa aaaatcggga
    10141 tagtgcgcgt aaaggtcgtg cacggtaaca ttgctgagct gcgtggccgg tttaagcagt
    10201 acccggtttg ccaccgagtc ctgagattcc ggatgggcat acagcagcaa aactttcgct
    10261 ggctgagaca tcatccccct cccggtatgg tttttgtgta tagtcgctgt tttgggctac
    10321 cattgcgccc ggtgcggcag ctcgcccata cattacatta tcataatgat aagttaacat
    10381 agtctgaaca tacggcgcct tatgattgtt ttctcctcgt tacaaattcg tcgcggcgtg
    10441 cgcgtcctgc tggataatgc caccgccacc atcaaccccg ggcagaaagt cggcctggtg
    10501 ggtaaaaacg gctgtggtaa atctaccctg ctggcattgc tgaaaaatga aatcagcgcc
    10561 gacggcggca gctacacctt tccgggaagc tggcaactgg cgtgggtgaa tcaggaaacg
    10621 ccagcgttac cgcaagcggc gctggaatat gtcattgacg gcgaccgtga atatcgtcaa
    10681 ctggaagcgc agctgcacga cgccaacgaa cgtaacgacg ggcacgccat tgcgaccatt
    10741 catagcaagc tggatgctat tgacgcatgg agtattcgct cccgtgccgc cagcctgctg
    10801 cacggcctcg gtttcagcaa tgaacaactg gagcgcccgg taagtgattt ctccggtggc
    10861 tggcgtatgc gtcttaacct tgcccaggcg ctgatttgcc gttcagactt gctgctgctc
    10921 gacgaaccga ctaaccacct cgatctcgat gccgttatct ggctggaaaa atggttgaag
    10981 agctatcagg gcacgctgat cctgatctct cacgaccgcg acttcctcga tccgatcgtt
    11041 gataaaatta ttcagatcga acaacaaagc atgttcgagt acaccggcaa ctacagttcg
    11101 tttgaagtac agcgcgccac ccgtctggcg cagcaacaag cgatgtacga aagccagcag
    11161 gaacgcgtgg cacatctgca aagttatatc gatcgtttcc gtgccaaagc caccaaagcg
    11221 aagcaggccc agagccgcat taagatgctg gaacgtatgg agctgattgc ccccgcacac
    11281 gtcgacaacc cgttccgctt tagcttccgc gcgccggaaa gcctgccaaa tccgttactg
    11341 aagatggaaa aggtcagcgc aggctatggc gatcgcatta ttctcgactc gattaaattg
    11401 aatctggtcc ccggctcgcg cattggtctg ttaggccgca acggcgcagg taaatcgaca
    11461 ttaatcaaac tgttagccgg tgaacttgcg ccagtcagcg gtgaaattgg tctggcaaaa
    11521 gggatcaagc tcggctactt cgcccagcat caacttgaat acctgcgcgc cgacgaatca
    11581 cctattcaac atctggcacg tttagcgccg caggagctgg aacaaaaact gcgtgactac
    11641 ctcggcggct ttggtttcct gggcgataaa gtaaccgaag aaacgcgccg cttctccggt
    11701 ggggaaaagg cccgcctggt gctggcatta attgtctggc agcgtccgaa tctgctgctg
    11761 ctcgacgaac cgactaacca ccttgacctc gacatgcgtc aggcagtcac cgaagcatta
    11821 atcgagtttg aaggcgcgct ggttgtcgtt tcgcacgacc gtcatttgct gcgttccacc
    11881 actgacgatc tctacctggt tcacgatcgt aaagtcgaac cgttcgacgg cgatctggaa
    11941 gattatcaac agtggttgag cgacgtacaa aagcaggaaa accagaccga cgaagcgcca
    12001 aaagagaacg cgaacagcgc ccaggcacgt aaagatcaga agcgtcggga agcggagctg
    12061 cgtgcgcaaa cccagccgct gcgtaaagag attgcccgtc tggaaaaaga gatggagaag
    12121 ctgaacgcgc aactggcgca ggcggaagag aaactcggcg acagcgaact gtatgaccag
    12181 agccgtaaag cggagttgac cgcctgcctg caacagcaag ccagcgccaa atccggcctg
    12241 gaagagtgcg aaatggcgtg gctggaagcc caggagcagc ttgagcagat gctgctggaa
    12301 ggccaaagca actgatggcg cagataacga cgaccgatgc caatgaattt agcagcagtg
    12361 ctgaattcac ccctatgcgc ggctttagca attgtcatct acaaactatg ctgccgcgtt
    12421 tgtttcgtcg tcaggtgaaa ttcaccccgt actggcagcg gctggagttg cccgacggcg
    12481 attttgtcga tctcgcgtgg agtgaagacc ccgcacaggc gaaacataaa ccgcgtttag
    12541 tggtgtttca cgggctggaa ggcagtctca atagccctta cgcccacggt ctggttgagg
    12601 cggcgcaaaa gcgcggctgg ctgggcgtgg tgatgcattt tcgcggatgc agcggtgaac
    12661 caaaccgtat gcaccgcatt taccattcgg gcgaaaccga agacgccagt tggtttttac
    12721 gctggctgca acgcgaattt ggtcatgcgc caacggctgc cgtcggctat tcgctcggcg
    12781 gtaatatgct ggcctgtttg ctggcaaagg aaggcaatga tctcccggtt gatgcggcgg
    12841 tgattgtctc tgcgccgttt atgctggaag cctgtagcta tcatatggaa aagggctttt
    12901 cccgcgttta tcagcgttac ttgctgaacc tgttaaaagc caatgccgcg cgcaagctgg
    12961 cagcctaccc cggaacgctg ccgattaatc tcgcgcagtt aaaatcggta cgtcgcatcc
    13021 gtgaatttga cgatctaatc accgccagaa ttcacggcta cgccgacgct atcgactatt
    13081 atcgtcagtg tagcgccatg ccgatgctga accggatcgc caaaccgacg ctgattattc
    13141 acgccaaaga cgatccgttt atggatcatc aggtgatccc aaaaccggaa agtctcccct
    13201 cgcaggtgga gtatcaactg actgaacatg gcggtcatgt tggctttatt ggcggtacat
    13261 tacttcatcc gcaaatgtgg ctggagtcac gcattcctga ctggttaaca acgtatctgg
    13321 aggcgaaatc atgttgattc cgtggcaaga cctctccccc gaaacgctgg aaaatttaat
    13381 tgaaagcttt gtgttacgtg aaggcaccga ttatggtgaa catgagcgta cacttgaaca
    13441 gaaagtcgcc gacgtcaaac gccagctaca gtgcggagaa gcggtgctgg tatggtcgga
    13501 actgcacgaa acggtcaata ttatgccgcg cagtcagttt cgcgaataac ctgcaaccgt
    13561 cggcggacga gataatagtg atagctaagt catggagtta ctatgtctgc caaacatccg
    13621 gtcattgcgg taacaggatc cagcggcgcg gggaccacca ccaccagcct cgcgtttcgt
    13681 aaaatattcg cgcagttaaa tctgcatgca gctgaggtgg aaggcgacag ttttcaccgt
    13741 tacacccgcc cggaaatgga catggcgatc cgcaaagcgc gcgacgccgg gcggcatatc
    13801 agctacttcg gccccgaagc gaacgacttc ggcctgctgg aacaaacctt cattgaatac
    13861 ggtcagagcg gcaaagggaa atctcgcaaa tatctgcata cctacgacga agccgtaccc
    13921 tggaatcagg taccgggaac attcaccccc tggcaacctt taccagaacc cactgatgta
    13981 ctgttttatg aaggtttaca cggcggcgta gtcacgccac agcataacgt tgcgcagcat
    14041 gtggacttac tggtcggcgt agtgcctatc gttaaccttg agtggattca aaaactgatc
    14101 cgcgacacca gcgagcgcgg gcactcacga gaagcagtga tggactcggt agtgcgttca
    14161 atggaagact atatcaacta catcacaccg cagttttccc gcacccatct taacttccag
    14221 cgcgttccca ccgtcgacac ttcaaacccg ttcgcggcaa aaggtatccc gtcgctggat
    14281 gaaagctttg tggtgatcca ttttcgtaat ctggaaggga tcgatttccc ttggctgctg
    14341 gcgatgttgc aaggctcatt catttcccac atcaatacgt tagtggtacc tggcggcaaa
    14401 atggggctgg caatggaatt aattatgctg ctgctggtgc aacgattgat ggaaggaaag
    14461 aaaatcgagt aactttgcta ttatgccgga taaaatacta tccggcttca caacgggata
    14521 gttaagtcac gcggcaacca cttcatacga gtgagtaata ttcaccgctt tttccagcat
    14581 caacgccact gaacaatatt tctcggcaga gagatcaacc gcacgcgcaa ccgctgcgtc
    14641 tttcaggtcg cgaccggtga cgataaaatg cagattaatg tgcgtaaaca ggcgtggtgc
    14701 ctcttcgcgg cgttcagagg tcaattttac ttcacaatcg accacatcct gacgcccttt
    14761 ttgcaggatc gaaaccacat cgatggcact gcaaccaccc gccgccatca acaccatttc
    14821 catcggactt ggtgctttat cgcctgagtt gccgtccatt aaaatctggt gaccagaggc
    14881 ggattcgccc agaaaagtta acccttcgac ccacttcact cgcgcttgca tttttgctac
    14941 tccactgcgt caattttcct gacagagtac gcgtactaac caaatcgcgc aacggaaggc
    15001 gacctgggtc atgctgaagc gagacaccag gagacacaaa gcgaaagcta tgctaaaaca
    15061 gtcaggatgc tacagtaata cattgatgta ctgcatgtat gcaaaggacg tcacattacc
    15121 gtgcagtaca gttgatagcc ccttcccagg tagcgggaag catatttcgg caatccagag
    15181 acagcggcgt tatctggctc tggagaaagc ttataacaga ggataaccgc gcatggtgct
    15241 tggcaaaccg caaacagacc cgactctcga atggttcttg tctcattgcc acattcataa
    15301 gtacccatcc aagagcacgc ttattcacca gggtgaaaaa gcggaaacgc tgtactacat
    15361 cgttaaaggc tctgtggcag tgctgatcaa agacgaagag ggtaaagaaa tgatcctctc
    15421 ctatctgaat cagggtgatt ttattggcga actgggcctg tttgaagagg gccaggaacg
    15481 tagcgcatgg gtacgtgcga aaaccgcctg tgaagtggct gaaatttcgt acaaaaaatt
    15541 tcgccaattg attcaggtaa acccggacat tctgatgcgt ctgtctgcac agatggcgcg
    15601 tcgtctgcaa gtcacttcag agaaagtggg caacctggcg ttcctcgacg tgacgggccg
    15661 cattgcacag actctgctga acctggcaaa acaaccagat gctatgactc acccggacgg
    15721 tatgcaaatc aaaattaccc gtcaggaaat cggtcagatt gtcggctgtt ctcgtgaaac
    15781 cgtgggacgc attctgaaga tgctggaaga tcagaacctg atctccgcac acggtaaaac
    15841 catcgtcgtt tacggcactc gttaatcccg tcggagtggc gcgttacctg gtagcgcgcc
    15901 attttgtttc ccccgatgtg gcgcagactg atttatcacc ccgatatcaa ctatgcactt
    15961 cgacaaacgc tggtgctatg tttgcccgtg gccgttgggt taatgcttgg cgaattacga
    16021 ttcggtctgc tcttctccct cgttcctgcc tgttgcaata ttgcgggcct tgatacgcct
    16081 cataaacgtt ttttcaaaca cttaatcatt ggtgcgtcgc tgtttgccac ctgtagctta
    16141 ctgacacagc tgctactggt aaaagatgtt cccctgccct ttttgctgac cggattaacg
    16201 ctggtacttg gcgtcactgc tgagctgggg ccattgcacg caaaattgct tcctgcatcg
    16261 ctgctcgccg ccatttttac cctcagtctg gcgggataca tgccggtctg ggaaccattg
    16321 ctcatctatg cgttgggcac tctctggtac ggattgttta actggttttg gttctggatc
    16381 tggcgcgaac aaccgctgcg cgagtcacta agtctgctgt accgtgaact ggcagattat
    16441 tgtgaagcca aatacagcct gcttacccag cacaccgatc ctgaaaaagc gctgccaccg
    16501 ctgctggtgc gccagcaaaa agcggtcgat ttaattactc agtgctatca gcaaatgcat
    16561 atgctttccg cgcaaaacaa taccgattac aagcggatgc tgcgtatttt ccaggaggcg
    16621 ctggatttgc aggaacatat ttcggtcagt ttgcatcagc cggaagaggt gcaaaaactg
    16681 gtcgagcgta gccatgcgga agaagttatc cgctggaatg cgcaaaccgt cgccgctcgc
    16741 ctgcgcgtgc tggctgatga cattctttac catcgcctgc caacgcgttt tacgatggaa
    16801 aagcaaattg gcgcactgga aaaaatcgcc cgccagcatc cggataatcc ggttgggcaa
    16861 ttctgctact ggcatttcag ccgcatcgcc cgcgtgctgc gcacccaaaa accgctctat
    16921 gcccgtgact tactggccga taaacagcgg cgaatgccgt tgcttccggc actgaaaagt
    16981 tatctgtcac taaagtctcc ggcgctacgc aatgccggac gactcagtgt gatgttaagc
    17041 gttgccagcc tgatgggcac cgtgctgcat ctgccgaagt cgtactggat cctgatgacg
    17101 gtattgctgg tgacacaaaa tggctatggc gcaacccgtc tgaggattgt gaatcgctcc
    17161 gtgggaaccg tggtcgggtt aatcattgcg ggcgtagcgc tgcactttaa aattcccgaa
    17221 ggttacaccc tgacgttgat gctgattacc accctcgcca gctacctgat attgcgcaaa
    17281 aactacggct gggcaacggt cggttttact attaccgcag tgtataccct gcaactgttg
    17341 tggctgaacg gtgagcagta catccttcca cgtcttatcg ataccattat tggttgtttg
    17401 attgctttcg gtggtaccgt ctggctgtgg ccgcagtggc agagcgggtt attgcgtaaa
    17461 aacgcccatg atgctttaga agcctatcag gaagcgattc gcttgattct gagcgaggac
    17521 ccgcaaccaa cgccactggc ctggcagaga atgcgggtaa atcaggcgca taacactctg
    17581 tataactcat tgaatcaggc gatgcaggaa ccggcattca acagccatta tctggcagat
    17641 atgaaactgt gggtaacgca cagccagttt attgttgagc atattaatgc catgaccacg
    17701 ctggcgcggg aacaccgggc attgccacct gaactggcac aagagtattt acagtcttgt
    17761 gaaatcgcca ttcagcgttg tcagcagcga ctggagtatg acgaaccggg tagttctggc
    17821 gatgccaata tcatggatgc gccggagatg cagccgcacg aaggcgcggc aggtacgctg
    17881 gagcagcatt tacagcgggt tattggccat ctgaacacca tgcacaccat ttcgtcgatg
    17941 gcatggcgtc agcgaccgca tcacgggatt tggctgagtc gcaagttgcg ggattcgaag
    18001 gcgtaataca ggctaaacca tcggataagg cattcacgcc gcatccgaca tattttgcct
    18061 gatgcgtctt atttacgccc caaccacctt cgccaccgcg tgggcgaaac gttgcatccc
    18121 ctcatcgata tccgcatctt ccaccaccag cgacggtgct aaacgcatca catccggtcc
    18181 ggcattcagc accattacgc caacctctgc acccgcatac aggaaatcac gcgcccgacc
    18241 tttgtagtgt ggtttcagct ccgcgccaat taacagcccc ataccgcgaa tatcgctaaa
    18301 cacatcgtac tgctgatcga tcttctgcaa atggtcaaca aaacgctggc gtttcgcctg
    18361 aatgccttcc agcacttcag gggtattgat gatatcaaac gccgcgcccg ctactgcaca
    18421 ggccagagga ttaccgccgt aggtggaacc gtgagaacca ggatgaaacg cagaagcaat
    18481 ttccgccgtg gtcagcatgg cgctaatcgg gaagccgccg cctaacgctt tcgcagaagt
    18541 cagaatatcc ggcgtcacac cgtagtacct gtaagcaaac aaatcgccag tccgccccat
    18601 cccgcactgc acttcatcaa acaccaataa tacctgatgt tgatcgcaca gctcgcgcaa
    18661 gccctgcaaa aactctggcg tcgctgccgt cacaccgccc tcgccctgga tcggctcaac
    18721 caccaccgca caggtgtgat catccatcac cgctttcact gcatggagat cgttaaaggg
    18781 aacgtggatg atgtctgccg gttgcggccc aaagccgtcg gaatattttg gctgcccacc
    18841 caccgaaacg gtaaacagcg agcgaccatg aaaagcgtta tggaaggcaa taattttggt
    18901 tttgaacggg ctatgacgca cacaggcgta atggcgtgcc agtttaaagg cggtttcgtt
    18961 agcttccgtg ccggagttca taaacaccac gcgttcggca aacgtcgcct caatcagttt
    19021 gcgcccaaga cgcagcgccg gttcattggt gaaaacgtta ctgatatgcc acagagtttc
    19081 gccctgggtt tttaacgcgt tcaccagcgc aggatggcaa tggcccaacg ccgtaactgc
    19141 aatgccaccc gcgaaatcga catactcctt gccttgctga tcccagattc ggctgccctg
    19201 accttttacc ggaataaact ctgccggagc ataaatcggc aggatcactt catcgaaagt
    19261 cgcgcgtgta attgctgttt gttcaattgc catctcatga tcaccctgtt acgcataaat
    19321 aaatatgaaa ttataatcac aaaatatgca taaaaaatca ctgaatggca atcggaaatc
    19381 agagatgcag gaaattagcc agcagttgat gtccttgttc gctaagaata ctttctggat
    19441 ggaactgcac accttccaga tcccactggc gatggcgaat ccccataatc tcgcgggttt
    19501 cgctccaggc cgtcacgtca aagcacgctg gtaatgagtc aggttccacc accagcgaat
    19561 ggtagcgtgt cacggtaagt ggatttgcca gcccccgaaa tacgccctca ccgctatgtg
    19621 taatcggcga ggttttgccg tgcatgacct ttgcggcgcg cacaactttg ccgccaaatg
    19681 cctgcgccat tgcctgatga ccgaggcaga cgccaagaat cggcaagcgc ccggcatagt
    19741 ggcgaataac gtcaagggag atcccggctt catctggcgt acaggggcca ggtgagatga
    19801 caattttttg tggtttaagg gcgtcgatat ccgccagcgt caacgcatcg ttgcgcttaa
    19861 ccagcacatc cgcccccagt tcacaaaagt actggtagag gttccaggta aaagaatcgt
    19921 agttatctat aagcaggatc atggcggctc cggtacgaaa gaaccgcact attttactca
    19981 gattccccgg cttcgcttac cactttgcta aatatcatct gcagtgcggt gagatccccc
    20041 attgccccac tctgatttgc ctgattccaa gcctcttttt cgataccctg ccagctcagt
    20101 tgatatccgg catgaatcgc cagttgctcg aagaagatcc gctgtgccag accacttccc
    20161 acccggaagg gatgcagcac gtcgatttca caatagtaat gcgccagccg ctcgacgaac
    20221 ttcgctttct ccaggccaac cagataacct tcttcctcca gatcctgcat cagggcattg
    20281 ccctcttttt cgatataagc aaagtggcag aacggcgtat cgccctgata aatatcaact
    20341 tcacgcagtt gccctgccca gtcgaaaata tcctggtaca gctggcgatg gatagtgcgc
    20401 aaatgcggta aaccgcgcac cagcggacca agctcaatgg tcgcagcacg cagcgccgtc
    20461 atttcgtaag cggcctgttc cagccgctgc tgctggcgga tgttcagccg gttacgcatg
    20521 atatcaaggc caggataaag atacggatcg cgcccttcgc cgaatttatc gctcatagtg
    20581 cctcctcagc tcttcaaggc gcgctaaagc ctctgcggca gtaagagtga ctaaaggcat
    20641 ctcgacgcct tcaagacggc gactggcctg gaaattacga ttacgctgaa gctcccagag
    20701 acgggacttt tgcttatcgg tgagtttctt cacttgatgc ctccctgaac gtgtccattt
    20761 gccacaagta taagcggcaa atccgggtta cgccgggaga gcagaagcgc gggcaagatt
    20821 gcccgcgcga gaaatcatta cggcaggact ttagcggaaa ggataactac cggttttgac
    20881 ggcacattct ggtacggacc aacatcatga gtcggcacct gggaaatctt atcggcaacg
    20941 tccatgcctt tcaccacttt accaaatacc gcgtaaccga aatcacgctg accatggtca
    21001 aggaaggcgt tatcggcaac gttgataaag aactggctgg tggcgctgtc tttgtcagcg
    21061 gtacgcgcca tcgcgatggt gccacgcgtg ttgcgcaggc cgttatcggc ttcatttttg
    21121 attggcgggt ttggtttttt ctgctgcatc tgctcggtga aaccgccgcc ctgaatcata
    21181 aagccaggaa tgacgcggtg aaaggtagtg ttgttataaa aaccgctgtt cacataatcg
    21241 acaaagtttt gcacagacac tggcgctttt tgtttatcca gctccagttc gatgttacca
    21301 gctgaggttg tcaacaatac gtgcgggtcc cctttcgctg ccattgctgc gggagaaaga
    21361 gcagaaagag cgaaaacagc agccatcgcc gccagggtcg atttgaacat gagatttcct
    21421 taacaaacag agcagaaaaa aagcaagtga agagattcta aatagccttc aagaacaagg
    21481 ccatcccttt acctaatttt acgtatctga aatatatgta acctgacgat cactttttat
    21541 tccgtaatca aaatcacctc ttaaaatgca atttagcaac cgattgcaat aaaacattta
    21601 aacagatcac aaaatcacct aaaatcgccc gctcgcggca tatcccgcta tgccatttta
    21661 tttttgaacg caggccaatc atgactaaca gcaatcgcat caagctcaca tggattagct
    21721 ttctctccta cgcactgact ggtgcgttgg ttattgtcac cgggatggtg atgggaaata
    21781 tcgccgatta tttcaatctg cctgtttcca gtatgagtaa caccttcacc ttcctcaacg
    21841 ccggcatttt aatctctatc ttcctcaacg cctggctgat ggaaatcgtc ccgttgaaaa
    21901 cgcagttacg ttttggcttt ctcctgatgg tgctggcggt tgccggtttg atgttcagcc
    21961 acagcctggc gctgttctcg acggcgatgt tcattctcgg ggtggtcagc ggcattacta
    22021 tgtcgattgg tacattcctg ataacacaaa tgtatgaagg gcgtcagcgc ggttcccgcc
    22081 tgttatttac tgactccttc ttcagtatgg ctgggatgat tttcccaatg atcgccgcgt
    22141 ttctactggc gcgcagcatt gagtggtact gggtttatgc ctgcatcggg ctggtgtatg
    22201 tcgctatttt tattctgacc ttcagctgtg agttcccggc actgggcaaa cgtgcaccaa
    22261 aaacggatgc tccggtagag aaagaaaagt gggggatcgg cgtactgttt ctctctgttg
    22321 cggcgctgtg ctacatcctc ggtcagttag gttttatctc ctgggtgcct gagtatgcca
    22381 aaggcctggg catgagcctg aacgacgcgg gcacgctggt gagtaacttc tggatgtcat
    22441 acatggtcgg catgtgggcg ttcagcttta ttcttcgctt ctttgatttg caacgcattc
    22501 tgaccgtact ggctggtctg gctgcgattc tgatgtacgt ctttaacacc ggaacaccag
    22561 cacatatggc gtggtcaatt ctcgctctgg gcttcttctc cagcgcgatc tataccacca
    22621 tcatcaccct gggttcacag cagaccaaag taccgtcgcc aaaactggtt aactttgtcc
    22681 tgacctgcgg aaccatcggt actatgttga cctttgtggt taccggcccg attgttgaac
    22741 atagcggtcc gcaggcggca ctgcttacag caaacggtct gtacgctgtc gtctttgtga
    22801 tgtgcttcct gttaggtttc gtcagccgtc accgtcagca taacaccctg acctctcatt
    22861 aattgctaat gccggacggc actatcgtcg tccggccttt tcctctcttc ccccgctacg
    22921 tgcatctatt tctataaacc cgttcatttt gtccattttt tgcacaaaca tgaaatgtca
    22981 gacaattccg tgacttaaga aaatttatac aaatcagcaa tatacccctt aaggagtata
    23041 taaaggtgaa tttgatttac atcaataagc ggggttgctg aatcgttaag gtaggcggta
    23101 atagaaaaga aatcgaggca aaaatgagca aagtcagact cgcaattatc ggtaacggta
    23161 tggtcggcca tcgctttatc gaagatcttc ttgataaatc tgatgcggcc aactttgata
    23221 ttaccgtttt ctgtgaagaa ccgcgcatcg cttatgaccg cgtacacctc tcgtcttact
    23281 tctctcacca caccgccgaa gagctgtcgc tggtgcgcga aggcttctac gagaaacacg
    23341 gcatcaaagt tctggtcggc gaacgcgcta tcaccatcaa ccgtcaggag aaggtgattc
    23401 actccagcgc cggacgtacc gttttttatg acaagctgat catggcaacc ggttcctacc
    23461 cgtggatccc accaatcaaa ggttctgata ctcaggactg ctttgtctat cgcaccattg
    23521 aagacctcaa cgccattgaa tcctgcgccc gtcgcagcaa acgcggtgcc gttgttggtg
    23581 gcggcctgtt aggtctggaa gccgctggcg cgctgaaaaa cttaggtatt gaaacccacg
    23641 ttatcgaatt tgcccctatg ctgatggcgg aacagcttga tcagatgggt ggcgagcagc
    23701 tgcgtcgcaa aatcgaaagt atgggcgtgc gcgttcacac cagcaaaaac acccttgaga
    23761 ttgtgcagga aggtgtcgaa gcgcgtaaaa ccatgcgttt tgccgacggc agcgaactgg
    23821 aagtcgactt tatcgtcttc tctaccggta tccgtccgcg cgataagctg gcaacccagt
    23881 gtggtctgga cgttgctccg cgtgggggga ttgtcattaa tgattcctgc cagacttccg
    23941 atccggatat ctacgccatc ggtgaatgcg caagctggaa caaccgtgta tttggtctgg
    24001 tagcaccagg ctacaaaatg gcgcaggtcg ccgttgacca tattctgggt agcgaaaacg
    24061 cctttgaagg tgctgacctt agcgccaagc tgaaactgct gggcgtagac gtaggtggta
    24121 ttggtgatgc gcacggtcgc acgcctggcg cacgtagcta tgtttacctc gacgaaagta
    24181 aagagatcta caaacgcctg attgtcagcg aagacaacaa aaccctgctc ggtgcggtac
    24241 tggtgggcga taccagcgac tacggcaacc tgctgcaact ggtgctgaac gctatcgagc
    24301 tgccggaaaa cccggattct ctgattctgc cggcgcactc gggtagcggt aagccgtcta
    24361 tcggtgttga taaactgccg gacagcgcgc aaatctgctc ctgcttcgac gtcactaaag
    24421 gtgatctgat tgctgccatc aacaaaggct gccacacagt tgcggcgctg aaagctgaaa
    24481 ccaaagcggg tactggctgc ggtggctgta tcccgctggt cactcaggta ctgaacgcgg
    24541 aactggcgaa acagggcatc gaagttaaca acaacctgtg cgaacacttt gcttattcgc
    24601 gtcaggaact gttccatttg atccgcgttg aaggcattaa aaccttcgaa gaactgctgg
    24661 cgaaacacgg caaaggctac ggttgtgaag tttgtaaacc aaccgtcggt tcgctgctgg
    24721 cctcctgctg gaacgaatac attctgaagc cggaacatac tccgctgcag gattctaacg
    24781 acaacttcct cgctaacatc cagaaagacg gcacctactc ggtgatcccg cgttctccgg
    24841 gcggtgaaat caccccggaa gggctgatgg cagtgggtcg tatcgcgcgt aaatttaatc
    24901 tctacaccaa aatcaccggc tcccagcgcc tggcgatgtt tggcgcacag aaaggcgatc
    24961 tgccggaaat ctggcgtcag ctgattgaag ctggcttcga aaccggtcat gcctatgcga
    25021 aagcactgcg tatggcgaaa acctgcgtgg gtagcacctg gtgccgctac ggtgttggcg
    25081 atagcgtcgg cctcggcgtg gaactggaaa accgctacaa aggcatccgt acgccgcaca
    25141 aaatgaagtt cggtgtctcc ggctgtaccc gtgaatgttc agaagctcag ggtaaagacg
    25201 tgggtattat cgccactgaa aaaggctgga acctgtatgt ttgcggtaac ggcggcatga
    25261 aaccgcgtca tgcggatctg ctggcagcgg atatcgatcg cgaaacgctg atcaaatatc
    25321 tcgaccgctt catgatgttc tacatccgta ccgccgacaa actgacgcgt accgcgccgt
    25381 ggttagaaaa cctcgaaggc ggcatcgatt acctgaaagc ggtgatcatt gacgacaaac
    25441 tggggctgaa cgctcatctg gaagaagaga tggcgcgcct gcgtgaagcg gtagtgtgtg
    25501 agtggactga aacggtcaat acaccgtccg cgcagactcg cttcaaacac ttcatcaaca
    25561 gcgacaagcg tgacccgaac gtgcagatgg tgccagagcg cgaacagcac cgtccggcaa
    25621 cgccgtatga acgtatcccg gtaactctgg tggaggacaa cgcatgagcc agtggaaaga
    25681 catctgcaaa atcgatgaca tcctgcctga aaccggcgtc tgcgcgctgt taggtgacga
    25741 gcaggtagcg attttccgcc cgtatcacag cgaccaggtg tttgcgatca gcaacattga
    25801 tccgttcttc gagtccagcg tgctatcacg cgggctgatt gcggaacacc agggcgagct
    25861 gtgggtcgcc agcccgctga aaaaacagcg ttttcgctta agtgatggct tgtgcatgga
    25921 agacgaacag ttttccgtca aacattacga agcacgagtg aaagacggcg tggtgcagct
    25981 gcgcggttaa tgttttaacg ggaggcgcaa tgcctcccct ttttgcatgg tcctgtaatg
    26041 accttcggta tattgcagga cattttttaa actttttgtt ttattttttg tttttatttt
    26101 ttaaaggata atcaaatgtt tacagacact attaataagt gtgcggctaa tgctgcgcgc
    26161 attgcacgcc tgtcggcaaa taacccgctc ggcttttggg tcagctccgc catggcgggc
    26221 gcatatgtgg gtcttgggat catcctgatt ttcacgctcg gtaatttgct cgacccgtcc
    26281 gtacgtccgc tggtgatggg cgcgaccttt ggtatcgcct taacgctggt gattatcgcc
    26341 ggttctgaac tgttcaccgg acacaccatg ttcctcacct ttggggtaaa agcgggcagc
    26401 atcagccacg ggcaaatgtg ggcaatcctg ccgcaaacct ggctgggtaa cctggtcggt
    26461 tccgtcttcg ttgccatgct ctatagctgg ggcggcggta gcctgctgcc ggtagatacc
    26521 agcatcgttc actccgtcgc gctggctaaa accactgcac cggcaatggt actgttcttc
    26581 aaaggtgcat tgtgtaactg gctggtttgc ctggcaatct ggatggcgct gcgcactgaa
    26641 ggggcggcga aatttatcgc tatctggtgg tgtctgctgg catttatcgc gtccggctac
    26701 gagcactcta tcgctaacat gacgctgttc gcgctctcct ggttcggcaa ccacagcgaa
    26761 gcctacacgc tggcgggtat tggtcataac ctgctgtggg tgacgctggg taatacttta
    26821 tcaggtgccg tattcatggg attgggttat tggtatgcta cgccgaaagc gaatcgtccg
    26881 gttgcggaca aatttaatca aactgaaacg gctgccggtt aattactaag gggtttttac
    26941 gtggatcatt tgcctatatt ttgccaatta cgcgatcgcg actgtctgat tgtcggcggt
    27001 ggtgatgtcg cggaacgcaa agcaaggttg ctgttagacg caggcgctcg cttaacggtg
    27061 aatgcattag cgtttattcc acagttcacc gcatgggcag atgcaggcat gttaaccctc
    27121 gtcgaagggc catttgatga aagccttctc gacacctgct ggctggcgat tgcagcgacg
    27181 gatgatgaca cgcttaacca gcgcgtcagc gaagccgctg aagctcgtcg catcttctgt
    27241 aacgtagtcg atgcgccgaa agccgccagc tttattatgc cgtcgattat tgaccgctca
    27301 ccgctaatgg tagcggtctc ctctggcggc acctctccgg ttctggcgcg cctgttgcgc
    27361 gaaaaactcg aatcactgct gccgttgcat ctgggccagg tggcgaaata tgccgggcaa
    27421 ttacgcgggc gagtgaaaca acagttcgcc acgatgagtg agcgtcgccg tttctgggag
    27481 aaattgttcg ttaacgaccg cctggcgcag tcgctggcaa acaacgatca gaaagccatt
    27541 actgaaacga ccgaacagtt aatcaacgaa ccgctcgacc atcgcggtga agtggtgctg
    27601 gttggtgcag gtccgggcga tgccgggctg ctgacgctga aaggactgca acaaattcag
    27661 caggcagatg tggtggtcta cgaccgtctg gtttctgacg atattatgaa tctggtacgc
    27721 cgcgatgctg atcgcgtttt cgtcggcaaa cgcgcgggat accactgtgt accgcaggaa
    27781 gagattaacc agatcctgct gcgggaagcg caaaaaggca aacgcgtggt gcggctgaaa
    27841 ggtggcgatc cgtttatttt tggccgtggt ggcgaagagc tggaaacact gtgcaacgcg
    27901 ggtattccgt tctcggtggt tccgggtatt accgcagctt ctggttgctc tgcctattcg
    27961 ggtattccac tcacgcatcg cgattatgcc cagagcgtac gcttaattac cggacactta
    28021 aaaaccggtg gcgagctgga ctgggaaaac ctggcggcag aaaaacagac gctggtgttc
    28081 tatatggggt tgaatcaggc cgcgactatt cagcaaaagc tgattgaaca cggtatgcct
    28141 ggcgaaatgc cggtggcaat tgtcgaaaac ggaacggcag tcacgcagcg cgtgattgac
    28201 ggtacgctca cgcaactcgg tgaacttgct cagcaaatga acagtccatc gctaattatt
    28261 attggtcggg ttgttggcct gcgcgataaa ctgaactggt tctccaatca ttaatttaac
    28321 ccgggccaga gaattctggc cttcttaatg actcttttta tatattcagc aaataaaaca
    28381 taacccctta ataaatatta tctgctaatg ggttatattc accgttacag cgacttacaa
    28441 aattaagtca ttcgctcact gacgccaatt aaatattcct gtatcgttct taacgagcag
    28501 tctgcttata ttcatataat caatgaatat taattaatat ataatacata gggaatgtaa
    28561 atgaacaaat ttattaaagt tgcactggta ggtgcagtac tggctacgtt aactgcatgt
    28621 actggtcata ttgaaaaccg tgataagaac tgctcttacg actacctgct gcacccggca
    28681 atttctattt ctaaaatcat tggcggttgc ggtcctactg cacagtaagt ctctgacaga
    28741 aatcggctaa caccggttta attgcccgac gccctctgga ttttccgagg gcgtatttgt
    28801 ttctatgtcc ttcttgcccc cgcccgtaaa aatattttca ctttaaattc aattttataa
    28861 ctacatccat tagccacagc gcgatcctgt gcgaaatttt gtgatcttcc tccacattac
    28921 acaacatcat atgttgttat attcatcatg cattgtcatg ttgcctttta aatgactgca
    28981 aactctcccc tacaacgtat tggacaagaa aaaggtatcg ctatgggaag ccaggaactc
    29041 caacgcaagc tcggattttg ggccgttctt gcaatcgccg tcgggacaac cgtcggctcc
    29101 ggtatttttg tatctgtggg tgaagtggca aaagcagcgg gcacgccgtg gcttacggtg
    29161 ctcgcgtttg tcattggcgg gttaattgtg atcccgcaaa tgtgcgtcta tgcggaacta
    29221 tccaccgctt atccggaaaa tggcgcagat tatgtttatc tgaaaaatgc cggaagccga
    29281 ccgctggctt tcctctccgg ctgggccagc ttctgggcca acgatgcgcc gtcattgtcg
    29341 attatggcgc tggcgattgt cagcaatctt ggctttttaa cgcctatcga tccgttgctc
    29401 ggtaaattta tcgccgccgg attaattatc gcctttatgt tgctacacct gcgctccgtt
    29461 gaaggcggcg cagcgtttca gacgctaatt accatcgcca aaattatccc gttcactatc
    29521 gtcattggcc ttgggatctt ctggtttaaa gcggagaatt ttgccgccct taccaccact
    29581 gcgattggcg caacgggcag ctttatggcg ctgctggcgg ggatctctgc caccagttgg
    29641 tcgtataccg gcatggcctc tatctgttat atgaccggcg aaattaaaaa ccccggaaaa
    29701 accatgccac gagcgctgat tggttcctgt ctgctggttc tggtgctcta caccctgctg
    29761 gcgctggtga tttccggcct gatgcccttc gacaaactcg ccaattctga aacgccgatt
    29821 tccgacgccc tgacctagat ccccgcactc ggcagcaccg ctgggatctt tgttgccatc
    29881 acggcgatga tcgtcattct tggttcgctt tccagctgcg tgatgtacca gccgcggctg
    29941 gaatacgcga tggcgaaaga caacctgttc tttaaatgct tcggccatgt gcatccgaaa
    30001 tacaacacgc cggatgtctc catcatcctg caaggggcgc tggggatctt cttcatcttc
    30061 gtttccgatc tcaccagcct gctgggttat ttcaccctgg tgatgtgttt caaaaatacc
    30121 ctcaccttcg gctccatcat ctggtgtcgt aaacgcgacg attacaaacc gctgtggcgt
    30181 actccggctt tcgggctgat gaccaccctc gccattgcgt caagcctcat tctggtcgcc
    30241 tcaacctttg tctgggcacc gattcccggc cttatctgcg ccgtcatcgt tattgctact
    30301 ggtctgcctg cttacgcctt ctgggcgaag cgtagccgcc agctcaacgc tttgtcgtaa
    30361 tgttatctga agaaaataaa atgttggata ttgataaaag caccgtggac tttctggtca
    30421 ccgaaaatat ggttcaggaa gtggaaaaag ttctcagcca tgacgttccg ctggtgcacg
    30481 ccatcgtgga agagatggtg aagcgcgaca ttgatcgtat ttatttcgtt gcctgcggct
    30541 cgccactcaa cgcggcgcaa acggcgaaac atctggcgga tcgcttttcc gatcttcagg
    30601 tctacgccat ttccggctgg gagttctgcg ataacacccc gtatcgcctc gacgatcgtt
    30661 gcgcggtgat tggcgtttct gactacggta aaacggaaga ggtaatcaaa gcgctggagc
    30721 tgggccgggc ctgcggcgca ctcactgcgg cgttcaccaa acgcgcggac agcccgatta
    30781 cctcggcggc ggaatttagc attgattatc aggccgactg tatctgggaa attcacctgc
    30841 tgctctgcta cagcgtggtg ctggagatga tcaccctcct cgcgccgaac gcggaaatcg
    30901 gcaagatcaa aaacgatctc aagcagttgc cgaatgcgct cggtcatctg gtacgcacct
    30961 gggaagaaaa aggccgccag cttggtgaac tggccagcca gtggccgatg atttataccg
    31021 ttgctgcggg tccgctgcgt ccgctgggtt acaaagaagg cattgtaacg ctgatggaat
    31081 ttacctggac gcacggctgc gtgattgaga gcggagagtt ccgccatggc ccgctggaga
    31141 ttgtcgaacc gggcgttccg ttcctgttcc tgctcggcaa tgatgaaagt cgccacacca
    31201 ccgaacgcgc cattaacttt gttaaacagc gtactgacaa cgtgatcgtc atcgattacg
    31261 ccgaaatttc gcaagggctg cacccgtggc tggcaccgtt cctgatgttc gtgccaatgg
    31321 agtggctctg ctactacctg tctatttaca aagatcacaa cccggatgaa cgccgctatt
    31381 acggtggtct ggtggaatat taatccctct cccggcccgg caacgggccg gatttacgca
    31441 aggagttacc cgatgaaaac aggtatgttt acctgcggcc accagcggct gcctattgaa
    31501 cacgcatttc gtgatgcaag cgagctgggt tacgacggca tcgaaatttg gggcggtcgc
    31561 ccgcacgcgt tcgcgccgga cttaaaagcg ggcggcatca aacaaatcaa ggcgctggcg
    31621 cagacgtatc agatgccgat tatcggttac acgccagaaa ccaacggcta tccgtataac
    31681 atgatgctgg gcgatgaaca tatgcgtcgt gaaagcctgg acatgatcaa gctggcgata
    31741 tatatggcaa aagagatgaa cgcgggttat acgctgattt ctgcggccca cgcgggctat
    31801 ctcacgccgc ctaacgttat ctgggggcgg ctggcagaga acttaagcga actgtgtgag
    31861 tacgcggaaa acatcggcat ggatctgatc ctcgaaccgt taacgccgta tgaatcgaac
    31921 gtcgtgtgta atgctaatga tgtgcttcat gcgctggcgc tggtgccttc gccgcgcctg
    31981 ttcagcatgg tcgacatttg cgcgccgtat gtccaggcgg aaccggtgat gagttatttc
    32041 gacaaactgg gcgataaatt acgtcatctg catattgtcg acagcgacgg ggccagcgac
    32101 acgcattaca ttcctggaga aggcaaaatg ccgctgcggg aactgatgcg cgatattatt
    32161 gatcggggct atgagggtta ctgtacggtg gagctggtga cgatgtatat gaacgagccc
    32221 agactctatg cccgccaggc gctggaacgc tttcgcgcgc tgctgccgga ggatgagaga
    32281 tgaaaaccct agcgacaatc ggcgataact gcgtcgatat ctacccgcaa ctgaataaag
    32341 cgttttctgg cggtaatgcg gtcaatgtgg cggtgtactg cactcgctac ggcatacagc
    32401 cgggatgcat tacctgggtg ggtgacgatg actacggcac aaagctgaag caggatctcg
    32461 cccgcatggg cgtcgatatc agccatgtcc atacgaaaca cggcgttacc gcacaaactc
    32521 aggtggaact gcacgacaat gatcgcgttt ttggcgacta caccgaaggc gtgatggccg
    32581 actttgccct gagtgaagag gattacgcct ggctggcgca gtatgacatt gtgcacgcgg
    32641 caatctgggg acatgcggaa gacgcattcc cacagctgca cgctgcgggc aaacttaccg
    32701 ctttcgactt ctccgacaag tgggacagcc cgctctggca gacactggtg ccgcatctcg
    32761 attttgcctt tgcctccgca ccgcaagaag acgaagcgct gcgtctgaag atgaaagcga
    32821 ttgttgcccg tggcgcagga acagtgattg tcacgctggg tgaaaacggc agcattgcct
    32881 gggatggcgc gcagttctgg cgtcaggctc ctgaaccggt gacggttatc gacaccatgg
    32941 gtgccggaga ttcgttcatt gccggattcc tttgcggctg gtctgcgggg atgacattac
    33001 cgcaggcgat ggcgcaggga acggcgtgcg cggcgaaaac cattcagtac cacggtgcct
    33061 ggtaggtata acgttggcgt gagcatcttc acgccaacgt gctgttactt gccggaaaac
    33121 gaccctataa tccgagtaat tcattcttta tttcagggtc gattatgtca gctacggacc
    33181 gctactctca tcaactcctc tacgctaccg tccgccagcg gctgctggat gatatcgcgc
    33241 agggggttta ccaggccggg caacagatcc ctaccgaaaa cgagctttgt acacaatata
    33301 acgtcagccg cattaccatt cgcaaagcca tcagcgactt agtggcagac ggcgtactga
    33361 tccgctggca gggaaaaggc acctttgtac aaagccagaa agttgaaaac gccctgctta
    33421 ctgtcagtgg ttttaccgat tttggcgtct cacaaggcaa ggcgacgaaa gagaaagtga
    33481 tcgaacagga acgggtcagc gccgcgccgt tttgcgaaaa gctgaacatc cccggaaaca
    33541 gcgaagtgtt ccatctctgc cgggtgatgt atctcgataa agagccgctg tttattgata
    33601 gttcatggat cccgctgtcg cgttatcctg actttgatga gatttacgtc gaaggaagct
    33661 ccacctatca gttatttcag gagcgttttg acacgcgagt ggtcagcgac aaaaagacca
    33721 tcgatatctt tgccgccacc cgcccgcagg caaaatggct gaaatgcgaa ctgggcgaac
    33781 cgttgtttcg catcagcaaa atcgcctttg atcagaatga caaaccggtg cacgtctccg
    33841 aactcttctg ccgcgccaat cgcatcacct taacgattga taataaaaga cattaaccgt
    33901 aggccggata agatgcgcca gcatcgcatc cggcgatgct ggcgcactga attttacatc
    33961 ccgcacgttc ccctcaccct aaccctctcc ccaaaggggc gaggggagcg ttcagtgcgg
    34021 aatttagctt gattgccgaa cggtgctatc cagcattaaa tactcgcaat actctcacgc
    34081 aaaatccgca gcaccgtctc ttcaccgctg cgattcgggt taatgcgaat cgcacaatgt
    34141 tcgagttgtg gattcgcctg gcgaaacgtt cctgaaagtc gatagaagag cggcgggatt
    34201 tcatatttcg actctgcacc gaccgggtaa ggcaacgcgc cacgcttttg cgcttcttcc
    34261 agcaccctgg cggcaatcag ctgatgaaac tcgacaatca acaccttcga ctgcgcattg
    34321 gcaatgacgg cgcttttcac ttccgccacc gcgccgccgt taagcaaagc cagcaaccgt
    34381 tcggatactc ccgcctgcac cgcatgcatc actggcgcga acaccagacc acgcaagact
    34441 tccagcgcct gtgagccctg gatctggcta ccgccggagt aaagcgtggc gcgaatccga
    34501 ctaataacat cggcatcgcc aaccactgca ccaacgccct ccggcccaaa cagcttgaag
    34561 caggagaatg tcgagacatt cgcgccgcat tcacagccga ttcgcgctac cttcatcacc
    34621 gcatagttat catcggttaa cgctggaaca cctgccgcgc gcaacgttgc cagcacatct
    34681 gccagcacat agctgtcctg cggctgctgg cgcgtatgct gcaccagcgc ggcatcaggt
    34741 tgttgctcgt cgacgacctg cttcagtgcc aacaggtcat tgaaatcagc agtaataagc
    34801 gtcagcccca tctgctcaat aataacccgt gtcgtcgggt aaacaggcgc gtcatgcacc
    34861 agaagacgct gccccggttt gagcaaagcc gccagcccgg cgcgaatcgc gccagtcccc
    34921 gcgccctgca ccagcgccgc ggcctgtgcg tgaaatgcat cagccagcac ctgctccacc
    34981 cgttgggtaa tacgcggttg attcagacct ggagttaacc ctaaatcacc gccagcaaga
    35041 aactcgctgc ctggaaaatg gcggcaaata gtgtccacca gcgcaaactg cttttgctgc
    35101 gcttcagcaa gagtcaggct ttgcaaagga aacgtcttca taggagcctt ccttaagccg
    35161 gaacgaacag gccaagccag taaagaacat tcagcagaat accggtgatc attactgcca
    35221 cgaccggtgc cgccattttc tgtaccggac ggcccaggga ttcgttgagg aagtaaatag
    35281 caaccgcgat agagaatccg gtataacccg ccatcttaat tgctgcgaaa atcgaaccga
    35341 ccagcagcgc catttccatc agcatattca tggcgttacg gatgttatcc gacgcattac
    35401 gcaccgacgg gtagcgtccc agccatttgc cgatcgaacg aagcagcaag acttccgccg
    35461 aaataaccac tgcgcctaat accgctgcaa ccatcggatt cggcgagaga tagcccaccg
    35521 cataaacaaa ggtaaagccc gcaactgcat acacacccgt tgctaaggcg gtggtggcaa
    35581 tcaacggcac aaaccccagt ccgcgcataa attctgccag agccgcctga ttaatcagcg
    35641 tttgcgattg ttccggcgtc acgcctgcgg aatacgcttt ctccagtgtg aagatcgaaa
    35701 cttcgctacc agcaaaaatc ttcatactgg caacggcggc aatcaatgct cccacgatgg
    35761 cgatataagg taagtttttg ataatccgcg acgtgcgctc ttcaaacacc gaaagcccgc
    35821 tggcatcgat gtcattttca tcacgatgac gcaggtcgtg agttatcgcg atccccagca
    35881 gcatcaccat gccaataaag atttcgatgg attcagggtt aagatgtggg aaatagcgca
    35941 cgactaccac acgggtcatc agaacaacca cggcggcgac cagactttgc ttccagccaa
    36001 actggtagaa aatcgccacc agcgggaaca gtgcaaaagc agaaaccacc ggcgagctta
    36061 attccccgag gctacctaat acatccaccg gcagcgcggt cagcagctgg ttaactggca
    36121 acaggcaagt aaggatcaac acgccccaga tagcgccaag accaaacgcc atcaggctgt
    36181 ttatcgccag tacgccgagg atatcggtag gaagaaagag taaccatgcg ttgagcagcc
    36241 aggttttcag ggtaaaagag atccccaccg aggccacaaa accaatgctc agaccaaaag
    36301 cgatactccc cgcctcgcga cggttcatat agccttcaat cagttgcggc aggatcgggt
    36361 ggatgccgtc atgaaaaaca gccgccgagc gatgcgccag aagcgatgtc atacccgtca
    36421 ggcacgccac cacgataatc tgaatataca gatccatagt cgtgcctttt atttcagatg
    36481 gttaatcaac atcggaatcg cgtgttcgac gtgctcaacg gaaaggccaa acgccacttt
    36541 cccttcagcg atcattttgg cgatatgctc gtctttcgct ttatgccgcg cccgcaccgg
    36601 tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt gccgcttcca
    36661 tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg gtttttttaa
    36721 tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc ttattggaaa
    36781 aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa tcctgactgg
    36841 cacaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc attggctttt
    36901 aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa cccacggtca
    36961 cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt cttaccaatg
    37021 gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc gaggttgtct
    37081 ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc ttgcaacaga
    37141 gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg gcgtccggtc
    37201 tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt aatctttcct
    37261 tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc gataccctgt
    37321 tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac cacatgttcc
    37381 gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc ggtttcgcgc
    37441 attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat ctcaatcaca
    37501 ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc atactgatca
    37561 aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg ctcatgggcc
    37621 agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg agggaggaaa
    37681 gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg gtagctccca
    37741 catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc tgataaacca
    37801 gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg ttaccgtgat
    37861 ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg gcaaggttac
    37921 ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga ccagcgaggt
    37981 cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat tcgtcgaggg
    38041 tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca taaggattgc
    38101 tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca tacagttttt
    38161 gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg aagccgttgt
    38221 cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg ctttctgcgg
    38281 catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc cgaccgacct
    38341 gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag atcacagaga
    38401 gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg ccaatcgcaa
    38461 ccgcctgatt gacccacaga aattgcagat catcgccacg gcgctccacc tgccagccag
    38521 cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc cgcagcggcg
    38581 gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca ccttcatgtt
    38641 gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct ggcgcataac
    38701 ccaatgtgtt gattagcccc agcttctcca acgttggtag ctgcaaatgc ggcaactggc
    38761 tcaggatgtg accacaggta ttcgccccgg catcttgcgg acgcaccagc gtgacatctt
    38821 tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc atttatgcct
    38881 tcagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg cgctgaatac
    38941 cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa cagagcacca
    39001 ctgcactgct caccggatac tcgcctgcca gcggcagggt atagtcgata ctgctgtcat
    39061 ccacggtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac accagcgcat
    39121 gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg ccacggaaat
    39181 gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg ccctgctggt
    39241 ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca ccgtattccg
    39301 ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc tcaatcgcaa
    39361 taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc ggtgttggca
    39421 aaactttccc ggcagcctca tcccaaagca ggcaagggaa atgggttagt ccggctaaat
    39481 gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc tgtacaaaac
    39541 cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc agcacaggct
    39601 ggacccgtcc ggccttaacc gctgccgcag aaacttcccg tgctttgtcg agagtaaaca
    39661 cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa gggatttgta
    39721 ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc gcctctttgt
    39781 aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc agccacggat
    39841 tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc gccgtctcaa
    39901 tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg tccggggcga
    39961 ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc tggcgtttca
    40021 atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata ttcatttttt
    40081 taaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt cgttagcagc
    40141 catccataat ccataaagat tggccagaaa atagccttct tcacacgggt taacttccag
    40201 agcgaattcc ttcaacaaca cctgatgcaa ttgcacaacg gcttgccagt ggctggattg
    40261 cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac ggcgactgcg
    40321 catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc gcaccggcag
    40381 atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga cgtccttatc
    40441 aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg cctttcgttt
    40501 aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc agggcgtgtt
    40561 tatctacaac ggcacgcagc aattgttgca ttgggtaatc atcgactcgc gtcaggacca
    40621 cggcttcggt ggccagtaaa aatcgcggat cgtctgttag cggcgtcgcc tctaatccca
    40681 gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca tcgcctttaa
    40741 caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca ctatcgccaa
    40801 aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct actcgcttaa
    40861 catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac gtatgcgggc
    40921 caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc gccaggcggg
    40981 aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata tccgcgccgc
    41041 gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg ctcgctaaac
    41101 cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg atatccacat
    41161 gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc ccacggcgtt
    41221 caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc aggccaaccg
    41281 atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg catttttcac
    41341 ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata aacgttcgac
    41401 gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa gataagtggc
    41461 gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt gcgaaaagag
    41521 gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta ttcgacaatg
    41581 cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac cctctcccca
    41641 aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa aaccaatcgc
    41701 ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg cgccgtcttt
    41761 catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt cctgcaattc
    41821 agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat acatcttgcc
    41881 ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa ggatatccag
    41941 caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg gctcgtcgga
    42001 gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca gcaggccgat
    42061 aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca gcagcgacat
    42121 tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct cgccatacag
    42181 cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct ggtcttcacc
    42241 caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca ccggatagtc
    42301 aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa actgcgtcat
    42361 gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact gcgcatgttc
    42421 tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac aagccagata
    42481 caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc gcacggtgat
    42541 cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt ttacccactg
    42601 acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg cgccactaaa
    42661 aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc cctaatgcgg
    42721 gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg atagcctcgc
    42781 cgtagttata tccgtaggtt aagccaactg atgggcaacc tgccgctttt gccgcctgaa
    42841 tatcattgcg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc atccgctcag
    42901 ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg ccgccgataa
    42961 ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg aacggcgtcg
    43021 gtttgttggt gaccaggcct agcggcaggc ctttagcctg caacgcgccc aacgtatcgg
    43081 caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag cgatcgaaca
    43141 gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg ggcggtttcc
    43201 ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg cgctccatca
    43261 gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg acgggcaact
    43321 ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg tcgaccagcg
    43381 taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg acttaccttt
    43441 gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc gaagattgcc
    43501 gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt gttcaccttc
    43561 acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg gcgacgtact
    43621 tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc agggttgacg
    43681 gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct cagaggtgtc
    43741 gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg cagcgtgcgg
    43801 tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc ggcagcgaaa
    43861 tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg ggcggtaatg
    43921 ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg aacatagtgg
    43981 ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt tgcggtatct
    44041 tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata ctgtttcatc
    44101 cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg tacaaagcca
    44161 gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt cgaactttga
    44221 cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg tgattggaga
    44281 tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga tgcgcttgtt
    44341 gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca gacagcggtg
    44401 cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg ctatcggcgt
    44461 aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac aactctgctt
    44521 ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg cgcagattgt
    44581 aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc gcccgacgga
    44641 acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg gcgcaatttg
    44701 tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc atcttcacaa
    44761 tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga gaaaagtcgg
    44821 tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg cccttgggca
    44881 aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc aaaaaagcgc
    44941 gatttttctt catgccgact aactaattac accttctccg gctgtggaga aagctccgac
    45001 agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga taaccttgat
    45061 tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt ggcctgaaca
    45121 tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta cacgccagaa
    45181 accaggacat accacggctg accattacgc gtcgtttcat agacaacgta gtttttcaga
    45241 ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct cagctgcaga
    45301 gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt cttccctcca
    45361 gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc cgtagtcgcc
    45421 gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc cacaggggca
    45481 gctggttcag tacgcttcgg cgtctgtgct accggcttcg gctccgtttt cacggttgct
    45541 tgcggttttt tcggttcaat caccgcctgc tgacgaactg ggcgcgtagt ggacggacgt
    45601 tcagcggttt gcgttttcgc cgtgtcacgc gatgcattgc cattgcgaac aggcgcaacc
    45661 gttgcgggtt cagtcggcaa cgtggaattg accgccacat tgttcagctg ttgctgattt
    45721 tgtggctggg tcagcgcatt gttcaggtca ccctgcactt caacacgttg ttgaccatcc
    45781 gttgccaccg gggtttgccc ttgagtcggc gtagaagaga tcggtggcag agaaacatcc
    45841 tgctgagtat tttccgcaga cgtggttccc ggcgctggct gcacgccatt cgcctgatcg
    45901 gtcgcattgc cagcaagatc aatactcttc tcgccagacg cggtttgatc gctggaagtg
    45961 gtcgaggggg cttttagcgc agaaccgata ccgatgatca acagcagtag aaccagaatg
    46021 ccgacgccca tcatcatata ctgacgagaa gcgggtttac tggctgcttt tttgcgctta
    46081 cgcggacgac gctctacgcg ctcttcatcc acggtttcat cttcggattc gtcaatttct
    46141 tcttcgattt ccggttcctc attgcgctct ttttgcgcac gagtcggacg gcgatcgtca
    46201 gtgtcatcaa gttcaatatc atcaaaattg atctgcggtt cgccacgttc agtacgctca
    46261 gaacgttcag aagattgacg agaacgacca gtacgacgat cgctgggatc gggtttcagc
    46321 tcgtcttctg gtttgaattc atccatttaa caccccacta aaaggttaat gcttaccacg
    46381 ttgcaattaa cctgaagcta atagaccgct tgataagcgg cctgaccttt cttgttgtta
    46441 cgctgattga caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc cgctgcgaac
    46501 ttcactctta ccaattgcca acggaagaat taagcgcatc tctcccgcaa ggactttctt
    46561 gtcacgcagc atatgcggta aatacgcctg cgcggacatt tcgcgcggcc cattgaccgg
    46621 taacccagcc cgcgtgagca gggttataat acgctgcgtt tcggcagaac taaactgccc
    46681 gagacgttcc gacgtccgcg ccgccatcac catacccgca gcgaccgctt caccatgtaa
    46741 ccaattgcca taccccattt cagcttcaat ggcatgacca aaggtgtgtc ccagattcag
    46801 taaagcacgt aacccggttt cgcgctcgtc ggcagcgaca acttctgcct tcagttcaca
    46861 acaacggcga atacagtacg ccattgccgg accgtccaga cgcaacaacg catccagatt
    46921 ctcttccagc cagttaaaaa acgcaccgtc aagaataatg ccgtatttga tgacttctgc
    46981 cagccccgac gctaactcac gcgggggaag cgttttcaga cagtcgagat ccaccaccac
    47041 tgaagcaggc tggtagaacg cgccaatcat gtttttaccg aggggatggt tgaccgcagt
    47101 tttgccgcca acggaggaat cgacctgcga cagtaacgtc gtcgggactt gaatgaaacg
    47161 aacaccgcgc tgataactcg ccgccgcgaa gccggtcaga tcgcccacta cgccgccgcc
    47221 aagcgccacc agcgtagtat cgcgaccatg cggcttttgt aacaacgccg taaagacggt
    47281 atcgagtaca gctaggcttt tatactgctc gccgtcaggg aggataacgc tatcgacgtt
    47341 aacacccgcc tgttcaagta cgccgcggac cttatcgaga tacagaggag ccagggtttc
    47401 gctggtgacc aacatgacct gctcgcccga tttcagcggt aagaatgaag ctggttcatt
    47461 aaacaaacca gatgcgatgg taattgggta actacgttcc ccgagagtaa cgacaatcct
    47521 ctccataacg cgacatccac cttaattact gtacccgcag acgagtgtat ataaagccag
    47581 aattagttgc tttccagcat gtgaataatc tggtttgcaa ccactttagc gctttgatca
    47641 tcagtacgaa tggtcacgtc ggcaatctct tcatacagcg gattgcgttc attggccaac
    47701 gcttccagaa cttcacgcgg cggtgtttca acgtgcagca acgggcgttt tttatcacgc
    47761 tgcgtgcgtg caagttgctt ttcgatggtc gtttcaagat aaacgacaac gccacgagcg
    47821 gaaagacggt tacgcgtttc acgggatttc acagagccgc cgccagtagc cagcacaata
    47881 ccctgtttct cggtcaactc attgatgacc ttttcttcgc gatcgcggaa gccttcttcg
    47941 ccttctaaat cgaaaaccca gcccacatca gctccggttc gtttctcaat ctcttgatcg
    48001 gaatcgtaaa attccatatt gagtttttga gctaactggc gcccaatagt gctttttccg
    48061 gcacccatag gcccaaccag aaagatattg cgtttctctg ccattttttc ggtactacta
    48121 agactattcg ttaatgataa acccgcttcg ctcagagagc gccgcaggac atgaactgaa
    48181 acctcataag atattgcgag agtcagactg aaaattatct caatactcca gcgggtttgg
    48241 caactgaata aatcacccat ccctctgcat atctggtcgc tgcaagcgca ctgccttgct
    48301 accaccgctc tggcgataaa tcaccgggta agattagcgt aaaaaagaca gcaaaatgcc
    48361 gcctgaatga taaatcatca tcatggaacg caggcagcga agagcaccaa ctctcaaatc
    48421 ggtactcctt gtatgctaaa tacctgcgcg cgtcaaatag atgaaacacg ttcagcataa
    48481 aaacattacc gcttttacgg ctgtttactc actggaaacc agtcgtggcg tgataaacac
    48541 cactaactcg cgtcgttcat cttcttttcc gtcatgacga aataattgcc cgaaccaggg
    48601 aatgtcgcca agcaacggta cgctatcctg acccgattta tttttacggg taaaaatgcc
    48661 gcccagcgcc aacgtttctc cgctttttac ctcgacctgc gtttcgatct cctgcttatc
    48721 aatcgccagc acttcgccat cggcctgctg tagcacctgc cccggaacgt tctggctgat
    48781 gtgtaatttc agccggatgc gacctttttg taacaccgtg ggcgtgacct ccatccccag
    48841 gacggcctct ttaaattcca ccgacgtcgc gccactttcc ccgctggaaa cctgatatgg
    48901 aatttcgctc ccctgtttaa tgctggcagg ctgaagatgt gaggccagca gacgcggact
    48961 ggcgataata tccagctgct gtttttgttc gagcgcggaa agctcaagat ccagcaagcg
    49021 tccgttgatg cgcccaatgt taaaaccgac atgcgttgtc gccgtcgcta cggagaggtc
    49081 gctaccaagc gtggtgactt gcccaacgcc accagcgtgt tgcgcatcgg ccagcgtcca
    49141 tttcacgcct aactcacgca aacttttttc attaatggtg acaatatgcg ccgacagctc
    49201 aacctgcccg accggcagat ccatttgcgc tacccactgt tcaagcgcgc ttaacgccgt
    49261 tttgttatct cgtagcaaaa ggcgattggt gcgtttatcg acggtcatac tccctttggc
    49321 actcagtagc ttctcccccg ctttcgccag ttctcccgcg tcggcgtatt gcagggttat
    49381 actgcgattt tccagcggca gatttgcctg cgcccgcgcc tgctccgcct cctggcgggc
    49441 gatattgtca ttctgccagg caatggaatg cactgagaga atgttgcctt cctgccgcgt
    49501 tatcagtccg gcgcttttca ctacagtttg tagtgcctgc ttccagggaa catctgttag
    49561 atgtaacgac accgtaccgc tgacgtctgg cgacacgacc aggttcaact tctcctgttc
    49621 agccagcgcc tgcaacacct gagctaccgg aacgtcatcc accatcagcg tcactttttg
    49681 cggctttgcc gcctgtacgc cggatatcag catcaacagt agtgcggcta tccattgctt
    49741 catttgtatc tccttgccgt tgccacaacc attgtggcgg ttcgcagttt gtcccggtac
    49801 ccagcgttag tacgtctggc gtcagctgta aaattgtcca gccgttttcc agcacatcgt
    49861 tttgctgcac ccgtcgccat ttcttttgcc cgtcttttat tacaccgatg atgcgctcgc
    49921 ctcgccctac catcccctga tagcgccact ggctaagttc gctaatccgg catagatctt
    49981 ccggcgattt aaaagggtca cgcataccgg ttaaaaggca caatgcaata cctgccaaca
    50041 accagcgttt aaccctcatg cggcatctcc agttgtagcg tgaacaaaag atcatcacct
    50101 tccacgctta acgaaaaacg gctcacgctg acgttgcgct ctgccagccg tgtaaatgcc
    50161 gacggcactg cttcccacag cgttttcaac gccaactcgc ctccctgcgc ggatggatgc
    50221 caggaaacca gttgcgcgcc ggataactga aaatccagtg gcgaaaaggg cagcgttttt
    50281 tcctcgctga agggagtggt gtctaccagg cgatacagtg cggcccactg ctggtgatga
    50341 cttgcccgta gtcgaattaa tgcgtcgcgc tcttcatggt gtgtcgatga tagaaaaatg
    50401 agcgtaacta acatcagcaa ccagaatgcc cagcaaaact ggcggaggcg gggtgatgtg
    50461 gcgaaccacc agtcaaagaa catgttcatc gctaaccttc cttgttaact gatactcaaa
    50521 ttgccagcgt ccctgcgcat cctgctgcgt ggctccccgc tgattgagat gaaaagacgc
    50581 atcctggcgg agtgacgttt ctagtgcgtt taacgcggta atgcttgttg tcagcccctt
    50641 gatctccagc gttccctgct gccagcttat cgttgtcagc caggcgtgct cgggtaaaag
    50701 cgccgccagt gcttccagcg cagattgcca gtcgcgggta aattgtcgct ggcgctgccg
    50761 ttgcgaacgc tgttcgcgta actgctgttg ctccagcaaa cgtggcttcg ttatctgtaa
    50821 gctgcgggcg agttgttgtt ccgcctgaag caaaactgcg tctatgcgcg cttcggcgct
    50881 gcctgtcaga cgcagtatta gcgttatccc gacggccagc agcagaggcg caacgaacat
    50941 cagcaaccag aaacgcagaa aagcggtccg gcgttgctgt cgccagggca aaaaattaat
    51001 tggcgggttc atcagtacgc cttcccaagc gccagcccca gcgcgatggc aaagtctcca
    51061 ccaggcggcg gtagcggcgg ctgacgaaca gaaacggcct cccaggggtc aaatccgcct
    51121 tcgccacata tcgcgacgct gtcgggatca acggataacg ctgccgccag ctccttcgcg
    51181 ctagtcatcc ccaccgccag tttgcgcccc cagctatagc gcgtcgccca cagccactgt
    51241 tcgttatcac gccaggccag acattgctga tgagaaggta aaaaaggcag gaatcgctgt
    51301 aatgcactgg catccggggt gatcgcacta acatgaacac gcaacctttc tgccagcgta
    51361 agcagcgttg ccagctcttt gctttgcgcg gcagtcacgt tataagcggg gctgagtgag
    51421 tcttcgctgt aatcgaagcg cagggagtcc ggatccatat ccagctcgcg ggccatcgtc
    51481 cctgacagcc aggccgtttg ctcccgctca ccaagggaca tcgacgggcg cggaaatgac
    51541 cgctgtaatg tgcgactggc gggaaacgcc aacataatgt gatgacgctg cggcagttcg
    51601 cgactccacg gtaacaacgt tttagccagc tgctgcgcat caacaatccg cccatctttg
    51661 ataatgtcgt gcgccagcgg caaccgccac cagcgttgca aaaagcattc ttttgcgccc
    51721 cgcacgatcg caaccgctgc cacttcttgc tgttgtaaat gcaaaccaat ttgccagatc
    51781 ttaaatgtca ttgtgatgat ctccttatca cccgtcactc tgacgggtat atcaatgcgt
    51841 ctggcttgcc tttatactac cgcgcgtttg tttataaact gcccaaatga aactaaatgg
    51901 gaaatttcca gtgaagttcg taaagtattt tttgatcctt gcagtctgtt gcattctgct
    51961 gggagcaggc tcgatttatg gcctataccg ctacatcgag ccacaactgc cggatgtggt
    52021 gacattaaaa gatgttcgcc tgcaaattcc gatgcagatt tacagcgccg atggcgagct
    52081 gattgctcaa tacggtgaga aacgtcgtat tccggttacg ttggatcaaa tcccaccgga
    52141 gatggtgaaa gcctttatcg cgacggaaga cagccgcttc tacgagcatc acggcgttga
    52201 cccggtgggg atcttccgtg cggcaagtgt ggcgctgttc tccggccacg cgtcacaagg
    52261 ggcgagtacc attacccagc agctggcgag aaatttcttc ctcagtccag aacgcacgct
    52321 gatgcgtaag attaaggaag tcttcctcgc gattcgcatt gaacagttgc tgacgaaaga
    52381 cgaaatcctc gagctttatc tgaacaagat ttaccttggt taccgcgcct atggtgtcgg
    52441 tgctgcggca caagtctatt tcggtaaaac ggtcgaccaa ctgacgctga acgaaatggc
    52501 ggtgatagcc gggttgccga aagcgccttc cacctttaat ccgctctact caatggatcg
    52561 tgccgtcgcg cggcgtaacg tcgtactgtc gcggatgctg gatgaagggt atatcaccca
    52621 gcaacagttc gatcagacac gcactgaggc gattaacgct aattatcacg cgccggagat
    52681 tgctttctct gcgccgtacc tgagcgaaat ggtgcgccag gagatgtata accgttatgg
    52741 cgaaagtgcc tatgaagacg gttatcgcat ttacaccacc atcacccgca aagtgcagca
    52801 ggccgcgcag caggcggtac gtaataacgt gctggactac gacatgcgcc acggctatcg
    52861 cggcccggca aatgtgctgt ggaaagtggg cgagtcggcg tgggataaca acaagattac
    52921 cgatacgctg aaggcgctgc cgacctatgg tccgctgctg cctgccgcag tcaccagcgc
    52981 caatcctcag ggagcgacgg cgatgctggc ggacgggtcg accgtcgcat tgagtatgga
    53041 aggcgttcgc tgggcgcgtc cttaccgttc ggatactcag cagggaccga cgccgcgtaa
    53101 agtgaccgat gttctgcaaa cgggtcagca aatctgggtt cgtcaggttg gcgatgcatg
    53161 gtggctggca caagtgccgg aagtgaactc ggcgctggtg tcgatcaatc cgcaaaatgg
    53221 tgccgttatg gcgctggtcg gtggctttga tttcaatcag agcaagttta accgcgccac
    53281 ccaggcactg cgtcaggtgg gttccaacat caaaccgttc ctctacaccg cggcgatgga
    53341 taaaggtctg acgctggcaa gtatgttgaa cgatgtgcca atttctcgct gggatgcagg
    53401 tgccggttct gactggcagc cgaagaactc accaccgcag tatgctggtc caattcgctt
    53461 acgtcagggg ctgggtcagt cgaaaaacgt ggtgatggta cgtgcaatgc gggcgatggg
    53521 cgtcgactac gctgcagaat atctgcaacg cttcggcttc ccggcgcaaa acattgtcca
    53581 caccgaatcg ctggcgctgg gttcagcgtc cttcacccca atgcaggtgg cgcgcggcta
    53641 cgcggtcatg gcgaacggcg gcttcctagt ggacccgtgg tttatcagca aaattgaaaa
    53701 cgatcagggc ggcgtgattt tcgaagcgaa accgaaagta gcctgcccgg aatgcgatat
    53761 tccagtgatt tacggtgata cgcagaaatc gaacgtgctg gaaaataacg atgttgaaga
    53821 tgtcgctatc tcccgcgagc agcagaatgt ttctgtacca atgccgcagc tggagcaggc
    53881 aaatcaggcg ttagtggcga agactggcgc gcaggagtac gcaccgcacg tcatcaacac
    53941 tccgctggca ttcctgatta agagtgcttt gaacaccaat atctttggtg aaccaggctg
    54001 gcagggtacc ggctggcgtg caggtcgtga tttgcagcgt cgcgatatcg gcgggaaaac
    54061 cggtaccact aacagttcga aagatgcgtg gttctcgggt tacggtccgg gcgttgtgac
    54121 ctcggtctgg attggctttg atgatcaccg tcgtaatctc ggtcatacaa cggcttccgg
    54181 agcgattaaa gatcagatct caggttacga aggcggtgcc aagagtgccc agcctgcatg
    54241 ggacgcttat atgaaagccg ttcttgaagg tgtgccggag cagccgctga cgccgcctcc
    54301 gggtattgtg acggtgaata tcgatcgcag caccgggcag ttagctaatg gtggcaacag
    54361 ccgcgaagag tatttcatcg aaggtacgca gccgacacaa caggcggtgc acgaggtggg
    54421 aacgaccatt atcgataatg gtgaggcaca ggaattgttc tgattaaaaa ggcgcttcgg
    54481 cgccttttca gttggctgac aaagtgcatt tgtttatgcc ggatgcgggt gaacgcgtta
    54541 tccggctaac aaaatcgtga aaaatcaata tattgcagga accccataag cctgataagc
    54601 gttgtgcatc aggcaaactt cacgcattta cactcgcccc tgccctttca accattcgcg
    54661 cacgaggaac agcgcgctga cattacgcgc ttcgttgaag tcagggtctt ccagcaaatc
    54721 catcatatgc gccagcggcc agcgcacctg tggtagcggc tctggctcat cgccttccag
    54781 cgattccggg tagagatctt gcgcgaccac gatattcatt ttactggaaa agtaagacgg
    54841 tgccatgctg agcttcttca aaaaagtcag atcgttcgca ccaaatccaa cctcttcttt
    54901 tagctcgcgg ttagcggctt cgtagacgct ttcacccgga tcaattaatc ctttcgaaaa
    54961 acctaattcg taggattcag ttcccactgc gtattcgcgg atcaggatca ggtgatcgtc
    55021 cacaatcggc acaatcatca ctgcttcccg gttggttgga cgcattcgtt cataaacacg
    55081 ccgcacgcca ttgctgaact ccagatccac actctcgacg gtaaacagtc gggaacgggc
    55141 tacagtttca acattcagaa tggtgggttt ttgtaatgat ttgttcatcg tgggatctat
    55201 gctgtgaaat cagcggttat tgtgcgatat cggacacgct ttcggcaatg tgaattgcat
    55261 gttatttaca tttatgtaac ttaataaata atcgtcctca aatcaaatta aaagtcaata
    55321 ggttgaaata actccaggaa tttgctgata ttccgccttc agagggtttg ctatgatcag
    55381 cggttactgt gatgtgctta atgatgctca agttaaactc cacgcttgcc gatagccaac
    55441 cgcagaatca tgtattgtgt ccggtgcgac tgaccacgcc tgacagacta agtaagatgg
    55501 ggaaagcatg agcaccattg tgattttttt agctgctttg ctggcctgct cactacttgc
    55561 gggatggctg ataaaagtgc gatccagacg gcgtcagctg ccctggacca acgccttcgc
    55621 ggatgcgcaa acgcgtaaac tcacacctga agaacgtagc gccgttgaaa attatcttga
    55681 gagcctgacg caggtattac aggtgcctgg cccaacggga gctagcgcgg caccgatctc
    55741 tctggcgctg aatgccgaaa gcaacaacgt catgatgctg acacacgcta tcacacgtta
    55801 cggcatctct accgacgatc cgaataaatg gcgttactac ctcgattcgg tagaagtcca
    55861 cctgcccccc ttctgggaac agtacatcaa cgatgagaat accgttgaac tgattcatac
    55921 cgattcgctg ccgttggtta tttcactcaa cggtcatacg ctgcaggagt acatgcagga
    55981 aacgcgcggc tatgccttgc aacctgttcc gtccacgcag gcgtcgattc gcggggaaga
    56041 gagtgagcaa atcgagctac tcaatattcg caaagaaacg catgaagaat atgcgctgag
    56101 tcgtccgcgc gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga tgttcttttt
    56161 ctgcctgatt accccggatg tatttgttcc gtggctggca ggcggcgcat tactgctgct
    56221 gggcgcaggt ctgtgggggc tattcgcgcc cccggcaaaa tcctccctgc gagaaattca
    56281 ttgtctgcgc ggtacacccc gtcgttgggg attgtttggc gaaaacgatc aggaacagat
    56341 caacaatatt tcactcggta ttatcgacct ggtctatccc gcacactggc agccatacat
    56401 tgctcaggat ctcggtcaac aaaccgatat tgatatctat ctagaccgcc acgtagtgcg
    56461 tcaggggcga tatctttcgc tgcatgatga agtaaaaaac tttccgttac agcactggct
    56521 gcgcagtacg attatcgctg cgggctcgct gctggtgctg tttatgctgt tattctggat
    56581 cccgctggat atgccgctga aattcactct ctcatggatg aaaggcgcgc agaccattga
    56641 agccaccagc gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata cattgcgtat
    56701 tagcggtacg ggaatgtgta atattcgcac ttccggaacc tggagcgcga aaaccaattc
    56761 acctttttta ccatttgact gctcgcagat catctggaat gacgcccgct cattaccatt
    56821 accagaatct gaactggtca acaaagcgac ggcattgact gaagcagtta atcgccagct
    56881 gcaccctaaa ccggaagatg aatctcgcgt cagtgcctca ttacgttcag cgattcaaaa
    56941 atccggcatg gtattgcttg atgattttgg cgacattgta ctgaagacag cggatttatg
    57001 ttctgccaaa gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg gcaacagtaa
    57061 agactgggac gcgctggtaa aacgcgccaa cgctgggaag ctcgatggcg tgaatgtgtt
    57121 attacgcccg gtgagtgcgg aatcgctgga taacctggtg gcaacctcca ccgcgccgtt
    57181 tatcacgcat gaaacggcgc gagcggcaca atcactaaac agtccggccc ccggcggatt
    57241 cctgattgtc agcgacgaag gcagcgattt tgttgatcag ccctggcctt cggcatcact
    57301 ttacgactac ccgccgcaag aacagtggaa cgctttccag aaactggcac aaatgctgat
    57361 gcatacgccg tttaacgccg aaggtatcgt cacaaaaatc ttcactgacg ccaatggtac
    57421 gcagcatatt ggccttcatc cgatcccgga tcgttccggc ctgtggcgct atctcagcac
    57481 cacattgctg ctactgacga tgctgggtag cgccatttac aatggcgtac aggcctggcg
    57541 tcgttaccag cgtcatcgca ctcgcatgat ggagattcag gcctattatg aaagctgcct
    57601 gaacccgcaa ctgatcaccc cttcagaaag ccttatcgaa taacacgttt gcgcggcagg
    57661 ttatgctacc ctgtcgcgca aattgcttca ctctggagat ttccctcatg catatcaaca
    57721 ttgcctggca ggacgtagat accgttctgc tggatatgga cggcacgttg ctcgacctcg
    57781 ccttcgataa ctatttctgg caaaagctgg tgcctgaaac atggggcgcg aaaaacgggg
    57841 ttacgccaca ggaagcgatg gaatatatgc gccagcaata tcacgacgta cagcatacgc
    57901 taaactggta ctgtcttgat tactggagtg agcaactggg tctggatatc tgtgcgatga
    57961 ccaccgagat gggaccgcgc gccgtactgc gtgaagatac cattccgttt cttgaggcac
    58021 tgaaagccag cggtaagcag cgaattttgc tcaccaatgc tcatccgcac aacctggcgg
    58081 taaaacttga gcatacgggt ctggacgcac accttgattt attactttcc acccacacat
    58141 ttggttatcc gaaagaggat cagcggttat ggcatgcggt ggccgaagct acgggtctga
    58201 aagctgaaag aacgctgttt attgatgaca gcgaagcgat tctcgatgct gccgcgcaat
    58261 ttggtattcg ttactgcctc ggcgtgacta atcctgattc cgggattgcc gagaaacagt
    58321 atcaacgcca tccgtcactg aatgactacc gccgcctgat cccctcgcta atgtgaagga
    58381 gacgccatga aagagaaacc tgctgttgag gttcgactgg ataaatggct atgggctgcc
    58441 cgtttttata aaacccgcgc gctggcccgt gaaatgatta aaggcggtaa ggtgcattac
    58501 aacgggcagc gcagcaagcc gagcaaaatc gtcgagctga atgccacgct cactctgcgc
    58561 cagggaaatg acgaacgcac ggtgattgta aaggcgatta ctgaacagcg tcgccccgcc
    58621 agcgaggcag ccttgctgta tgaagagact gcggaaagtg tagagaaacg cgaaaaaatg
    58681 gcgatggcac gtaaacttaa tgccttaacc atgccgcacc cggaccgacg cccggacaaa
    58741 aaagagcgcc gcgacctgtt acgatttaaa cacggcgaca gtgaataact gtcacctgca
    58801 agagagatga ttatgccgca acatgaccaa ttacatcgct atctgtttga aaattttgcc
    58861 gttcgcggcg aactggtaac cgtttcggaa accctgcaac agatccttga gaaccacgat
    58921 tatccgcagc ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag cctgttaacc
    58981 gctacgctga agtttgatgg tgatatcacc gtacagctgc agggcgacgg tccgatgaat
    59041 ctggcggtta ttaacggtaa caataaccag caaatgcgcg gtgtggcgcg cgtgcagggc
    59101 gaaattccag aaaatgccga cctgaaaacg ctggtcggca atggttacgt ggtgatcacc
    59161 attaccccga gcgaaggcga acgctatcag ggcgtagttg gtctggaagg tgataccctg
    59221 gcggcctgcc tagaagatta ctttatgcgt tctgaacagc tgccgacgcg cctgtttatt
    59281 cgcaccggcg acgtagacgg caaaccggct gcaggcggta tgttgttgca ggtaatgcct
    59341 gcgcaaaata cccagcagga cgactttgac cacctggcga cgctaaccga aaccatcaaa
    59401 accgaagaac tgctgacctt accggcaaac gaagtgttgt ggcgtttgta tcacgaagaa
    59461 gaggtgacgg tttacgatcc gcaggatgtg gagttcaaat gcacctgctc gcgtgaacgt
    59521 tgcgccgatg cgctgaaaac gctgcctgat gaagaagttg atagcatcct ggcggaagat
    59581 ggcgaaattg acatgcattg tgattactgc ggtaaccact atctgttcaa tgcgatggat
    59641 attgctgaaa tccgcaacaa cgcgtctccg gcagatccgc aagttcatta atgagtatgt
    59701 ccggcagaga gggctctgcc ggattttacg acttaatggc ctgatgtgct gcgcttacca
    59761 gcgcctatga aataaatcgc aaattatttt ggtcagatac agcgtcgtat ccgataaaag
    59821 cgttgtcact tacttaagtt tatttccagc gtttcaccat ctaccgtcag atcgcgccac
    59881 gctttcggcc tcgtccccag ttttccatcc ttcgcagctg tgagtaattt ttctttcgtt
    59941 atttcgttcg gaagtaaact catatcaagc gcgtcccatt cttttctgtc tgagtcaaag
    60001 ccatagacaa tgactctgtc atcattaaaa gcaaacagga tccgctccgc ctgaccatca
    60061 ctattcagat cttgctcgac cagtacgcag gcgtcttttt caatacaggt catcacgtta
    60121 tagcgatcct ggattaaggc cgaccagaat gtcgcatccg gtttaacaga accaggggca
    60181 attaacacat tttcggctaa tactttttcc gatacctgtt cctgaagatg ttgctctcca
    60241 tctaacgcca tcagcagatc ccgcgcgcgt ttcgggtctt tcataaaccc ggcatcgctt
    60301 ttcagcgact caagcgccgc acgtccatag cgaccgctct gttcgagcat gtagatagtt
    60361 acctggtctg gcgtgttttt gccgctctga taacgcgcca tatgactgtt cacgctaata
    60421 cgcatactgt ccagcaccgg cgaattaaga agcaccagta tcaccaacac caataacgaa
    60481 accgcaaggt tcactttgcc ctgaagaaca aggggatttt gcccattacg ccagacgata
    60541 ctgacaaaat accccagcga ccacaccagt aacaccagct ccgccagcgc gccctgcaag
    60601 cggtcgacag tccagccgta ctgagcgacc cgcagccata acccccaggt ggcgacgaac
    60661 acatacagcg gagcaaccag caaagcggtt ttaatcaggc aacgcaacgg ccccgtccag
    60721 ggaagtgacg ctttttgcgg atcgcggaca atagccatta agatcaattg caaaaaggcc
    60781 agcgttaaca gcaacccggc ggcggagatg tggcgagaaa tcgcgctcag gcccgtaaac
    60841 ggcagggtga tgataaacat cagggttagc aatgatacta acggcagcaa ccccgtggcg
    60901 attagcgtga acaacttttg aatagagtcg attaaacgtg actgtgtacg cgcgaggatc
    60961 accgccagtg cggtaaccag accaaatgta agatacataa accagtcggt tgcaaaaaag
    61021 agcgtattga aaaacgtgat gccaacaagt ttaaacaact caccccacag taaaagcacc
    61081 agccacgtca ggccattgga gagaaaaatc accagtaata taagtacgtt atgccatact
    61141 gactggtaaa agtagctata gcggctactg ccattgcgga tacgaaggct ttgttgtatc
    61201 cacggtagca acaacattgc catcaacagc agatagcagc caaaatccca aatcgccttt
    61261 tcagctctcc aggggttcat gccatcagtt tgccacttca gccaaccgct catccctgtc
    61321 gtggcaataa acaccagcgc cagccatccc caaaggcgct tctgtttaaa agagatcacg
    61381 gagaagagta aaacggatga aaacgcgacg gtcgcaggca cgccataaac aatccaactg
    61441 tgattttttc cggcaagcca ggcgataagc aggtagcaaa ccagcccctg aagtaatccg
    61501 gtagcaatca tcccccagcg tgttgccggt gaaagttcaa cgttatccat gttggttatc
    61561 cagaatcaaa aggtgggtta attatcgcat ccgggcagta gtattttgct tttttcagaa
    61621 aataatcaaa aaaagttagc gtggtgaatc gatactttac cggttgaatt tgcatcaatt
    61681 tcattcagga atgcgattcc actcacaata ttcccgccat ataaaccaag atttaacctt
    61741 ttgagaacat tttccacacc taaaatgcta tttctgcgat aatagcaacc gtttcgtgac
    61801 aggaatcacg gagttttttg tcaaatatga atttctccag atacgtaaat ctatgagcct
    61861 tgtcgcggtt aacaccccca aaaagacttt actattcagg caatacatat tggctaagga
    61921 gcagtgaaat gcgcgttaac aatggtttga ccccgcaaga actcgaggct tatggtatca
    61981 gtgacgtaca tgatatcgtt tacaacccaa gctacgacct gctgtatcag gaagagctcg
    62041 atccgagcct gacaggttat gagcgcgggg tgttaactaa tctgggtgcc gttgccgtcg
    62101 ataccgggat cttcaccggt cgttcaccaa aagataagta tatcgtccgt gacgatacca
    62161 ctcgcgatac tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac aaacctctct
    62221 ctccggaaac ctggcagcat ctgaaaggcc tggtgaccag gcagctttcc ggcaaacgtc
    62281 tgttcgttgt cgacgctttc tgtggtgcga acccggatac tcgtctttcc gtccgtttca
    62341 tcaccgaagt ggcctggcag gcgcattttg tcaaaaacat gtttattcgc ccgagcgatg
    62401 aagagctggc aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag tgcactaacc
    62461 cgcagtggaa agaacagggt ctgaactccg aaaacttcgt ggcgtttaac ctgaccgagc
    62521 gcatgcagct gattggcggc acctggtacg gcggcgaaat gaagaaaggg atgttctcga
    62581 tgatgaacta cctgctgccg ctgaaaggta tcgcttctat gcactgctcc gccaacgttg
    62641 gcgagaaagg cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa accacccttt
    62701 ccaccgaccc gaaacgccgc ctgattggcg atgacgaaca cggctgggac gatgacggcg
    62761 tgtttaattt cgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa gaagcggaac
    62821 ctgaaatcta caacgctatc cgtcgtgatg cgctgctgga aaacgtcacc gtgcgtgaag
    62881 atggcactat cgactttgat gatggttcaa aaaccgagaa cacccgcgtt tcttatccaa
    62941 tctatcacat cgataacatc gttaagccgg tttccaaagc gggccacgcg actaaggtta
    63001 tcttcctgac tgctgatgct ttcggcgtgt tgccgccggt ttctcgcctg actgccgatc
    63061 aaacccagta tcacttcctc tctggcttca ccgccaaact ggccggtact gagcgtggca
    63121 tcaccgaacc gacgccaacc ttctccgctt gcttcggcgc ggcgttcctg tcgctgcacc
    63181 cgactcagta cgcagaagtg ctggtgaaac gtatgcaggc ggcgggcgcg caggcttatc
    63241 tggttaacac tggctggaac ggcactggca aacgtatctc gattaaagat acccgcgcca
    63301 ttatcgacgc catcctcaac ggttcactgg ataatgcaga aaccttcact ctgccgatgt
    63361 ttaacctggc gatcccaacc gaactgccgg gcgtagacac gaagattctc gatccgcgta
    63421 acacctacgc ttctccggaa cagtggcagg aaaaagccga aaccctggcg aaactgttta
    63481 tcgacaactt cgataaatac accgacaccc ctgcgggtgc cgcgctggta gcggctggtc
    63541 cgaaactgta atgatttgaa gctggagaat atctatccag tatcttatag aaagcaaaac
    63601 gggaggcacc ttcgcctccc gtttatttac ccttcttttg tcgtgccctg cgcccgcgtt
    63661 accggcactg gcagccaggc gcgaatggaa agcccgcccc gctcgctggt gccaagctcc
    63721 agcatcccgt tatggttatc cacgatacgc tgcacaattg ccagccctaa tcccgtgccg
    63781 ctaatggtgc gcgcactgtc gccgcggaca aacggctgga acaggtgctt acgttgttcc
    63841 ggcgcaattc ccggaccgtc atcttccacc tggaaccagg cgcgattcgg ctccgttccg
    63901 ctgctgactt tgatccagcc attgccataa cgggcggcgt tgaccaccat attcgccacc
    63961 gcgcgtttga tcgacagcgg gtgcattttc acttcaatgc tgccggggta aagcgcggtt
    64021 tcaatttccc gctcatagcc actttcggca gcaatcacct caccgagcac tgcattaaga
    64081 tccgccattt ccatcggcat ctcctgcccg gtgcgcaggt agtcgataaa ctgctcaatg
    64141 atggcgttgc actcttcgat atctttattg atcgattctg ccagatagcc atcctgctcg
    64201 ctcatcatct cagtcgccag gcgaatacgc gtcagcggcg tgcgcaagtc gtgacttacc
    64261 cccgccatca gcagcgtgcg gtcatccgcc agttgcttaa caccagccgc catatggtta
    64321 aaggcacggg taacggaacg cacctccgaa gcgccatatt cacgcagcgg cggcggaata
    64381 atccctttac caacctgcaa ggctgcgtgt tcgagatcga ccaacggtcg gttctggata
    64441 cgaataaaca gccacgcccc gcctatcgcc aatagcataa tcgccagcgt atagcggaac
    64501 agcggagaga aatcgccctg atgaatttcg gtcagcggca cgcgtaccca gatattgggc
    64561 gacagccagg ttttcagcca gacgacaggc gaacttttgt tgacctcaac gcgcacttcc
    64621 gtcgggccgc ccagttgctg tgccatctga tggcttaaga attcatagtg ttgcgcccaa
    64681 cgcagacctg cctcttcggc agcctcgttg gagtagagag agatccccag ctcacggtag
    64741 atctcccgac ggaaagcggg aggcacaacc aactgcgtgc cgtcctccag ttgcagttta
    64801 tcggtcatca acatacgcac ttcgtacgcg aggactttat taaactgctg gaggctcggc
    64861 aaaatcgcga agttcagcac caccagataa gtcgtcacca ggctggcgaa cagcaaggtg
    64921 acgatgagca ataacgtacg ggcaaatgaa cttcgtggcg agaagcgcaa tcgcctcatg
    64981 ctttagagcc gtccggtaca aagacgtagc ccaggcccca aacggtctga atgtaacgcg
    65041 gatgcgctgg atcttcttct accatgcggc gcagacgcga aatctgcacg tcgatggagc
    65101 gttccattgc ggaatattca cgaccacggg caaggttcat cagcttatcg cgggagagcg
    65161 gctcacgcgg atggctgacc agtgccttca gtaccgcaaa ctcaccgctg gtgagcggca
    65221 tcggctcatc ttcgcggaac atttcgcgcg taccgaggtt aagtttgaac ttaccgaaag
    65281 caattaccgc ctcttcctgt gacggtgcgc ctggcagttc gttcgcctga cgacgcagca
    65341 ccgcacgaat acgggccagc agttcacgcg ggttaaacgg ttttggaatg tagtcgtcag
    65401 cgccaatctc caggcctacg atacggtcca cttcttcccc tttcgccgtc accataatga
    65461 tcggcatcgg gttgctctga ctacgaagac gtcggcaaat cgacaagcca tcttcaccag
    65521 gtaacattaa atccagtacc ataagatgga aagattcacg agtcagcagg cgatccatct
    65581 gttctgcatt agcgacgctt cgaacctgga agccttgttc ggtgagataa cgttccagca
    65641 gcgcacgcag gcgcatgtcg tcatcgacca ccagaatctt gtagttctct tgcattgttt
    65701 gtactcccaa aggttcgcaa caatttgtaa gcgtgtattc ttaaaaaagc tcacgttcgt
    65761 caccagctaa atctggtatg aatttcagcc taaattgtta caaagcatat taaacagcag
    65821 cttaagtata caatttattc ggcgaaacat tattgattct gttgatatga tcacgttata
    65881 cccaatgtgc gcattatcaa acagacaaag ggaatcaacg agatgaaaac gcccctggtt
    65941 acccgggaag ggtatgaaaa actcaaacaa gagcttaatt atctctggcg tgaagaacgc
    66001 ccggaggtca caaaaaaggt gacctgggcc gcaagtctgg gcgaccgcag cgaaaatgct
    66061 gactatcagt ataataaaaa gcgtctgcgt gaaatcgacc gtcgcgtgcg ctatctcact
    66121 aaatgcctgg aaaatctcaa aattgtcgat tactcccctc agcaggaagg caaagtcttt
    66181 tttggcgcgt gggtggagat tgaaaacgac gatggcgtga ctcaccgttt ccgtattgtc
    66241 ggctacgatg aaatttttgg ccgtaaagat tacatctcta tcgattcccc gatggcccgc
    66301 gcattgctga aaaaagaagt cggcgatctg gcggtggtga atacccctgc cggggaagcg
    66361 aactggtatg ttaatgctat cgagtacgtg aaaccgtaag gaagagtctt aacctcctgc
    66421 cgatggctgg catttttgcc agccagtccg tataactatc ccctgatttt tgatccgaaa
    66481 agatgaactc aaaccatgat gaatgattcg ttctgccgca ttattgcggg tgaaattcag
    66541 gcgcgcccgg aacaggttga cgctgccgtt cgcctgcttg atgaagggaa taccgtgccg
    66601 tttatcgcac gttatcgtaa ggaaatcacc ggcggtctgg atgacacgca gctgcgtaat
    66661 ctggaaacgc gtctgagcta tctgcgcgag ctggaagaga gacgtcaggc gatcctcaaa
    66721 tccatttccg agcaaggcaa actcaccgat gatctggcga aggccatcaa cgccacccta
    66781 agcaaaaccg aactcgaaga cctctacctg ccctacaaac ctaaacgccg cacccgcggg
    66841 caaatcgcca ttgaagcagg gcttgagccg ttggctgacc tgctgtggag cgatccgtca
    66901 cacacgccag aagtcgccgc tgcacaatat gttgatgccg ataaaggcgt ggcagatacc
    66961 aaagccgcgc tggatggcgc gcgctatatc ctgatggaac ggtttgccga agatgccgcg
    67021 ctgctggcga aagtgcgtga ttatctgtgg aagaacgcgc atttggtttc tacggtggtg
    67081 agcggtaaag aagaggaagg ggcgaaattc cgcgactatt tcgatcatca cgaaccgttg
    67141 tccacggtgc cttctcaccg cgcgctggcg atgttccgtg ggcgtaacga aggcgtactc
    67201 cagctttcgc tgaatgccga tccgcagttc gatgagccgc ccaaagagag ctattgcgag
    67261 caaatcatca tggatcacct tggcctgcgc ctgaacaatg ccccggcgga tagctggcgc
    67321 aaaagcgtag tgagctggac ctggcgcatc aaggtgctga tgcatctgga aaccgaactg
    67381 atgggtaccg tgcgcgaacg cgcggaagat gaagcaatca acgtctttgc ccgtaacctg
    67441 cacgatctgc tgatggcggc ccctgccgga ctgcgtgcaa cgatgggcct cgatccgggt
    67501 ctgcgtaccg gggtaaaagt ggcggtggtc gatgccactg gcaaactggt ggcgaccgat
    67561 accatttacc cgcacaccgg acaagccgca aaagcagcga tgaccgttgc tgccttgtgt
    67621 gaaaaacata acgttgaact ggtggcgatc ggcaacggta cagcttcccg cgaaactgaa
    67681 cgtttctatc tcgacgtgca gaagcagttc ccgaaagtga ccgcacagaa agtgatcgtc
    67741 agcgaagctg gcgcgtcggt ttactcagct tccgagctgg cagcgcagga gttcccggat
    67801 ctcgacgttt cgctgcgtgg tgcggtatct atcgcccgcc gtttgcagga tccgctggcg
    67861 gagctggtga aaatcgatcc gaaatctatc ggcgtaggtc agtatcagca tgacgtcagc
    67921 cagacgcaac tggcccgcaa actggatgca gtagtagaag actgcgtaaa cggcgttggc
    67981 gtcgatctca acaccgcttc tgttccgcta ttaacccgcg tggcgggcct gacgcgcatg
    68041 atggcgcaaa acatcgtggc ctggcgcgat gagaacggcc agttccagaa ccgtcagcaa
    68101 ctgctgaaag tcagccgtct ggggccgaaa gccttcgagc agtgcgcggg cttcctgcgc
    68161 attaaccacg gtgataaccc gctggacgcc tctaccgttc atccggaagc ctatccggtg
    68221 gtggaacgca ttctggcagc aacacagcag gcactgaaag atctgatggg taacagcagc
    68281 gaactgcgta acctgaaagc gtctgacttt actgatgaaa aattcggtgt gccgacagta
    68341 actgacatca tcaaagagct ggaaaaaccg ggtcgcgatc cgcgtccgga atttaaaacc
    68401 gctcagtttg ccgatggcgt cgagacaatg aacgacctgc aaccgggtat gatcctcgaa
    68461 ggcgcagtga ccaacgtcac caactttggc gcgtttgtcg atattggcgt gcatcaggac
    68521 ggcctggttc acatctcttc attgtcgaac aagtttgtgg aagatccgca taccgtggtg
    68581 aaagcgggcg acattgtgaa ggtgaaagtg ctggaagtgg atcttcagcg taaacgtatc
    68641 gccctgacta tgcgtctgga cgagcagcct ggcgaaacca actctcgtcg cggcggcggt
    68701 aatgaacgcc cgcaaaacaa ccgcccggca gccaaaccac gcggtcgtga agcgcagcct
    68761 gccggtaata gcgcgatgat ggatgcgctg gcggcagcga tgggtaaaaa acgttaaacg
    68821 cccggactgg cccctaaacc tgcgcgataa ggcgttcacg ccgcatctgg caacgaacgc
    68881 tttgcctgac ataaaagtgc cggagaatat ctccggcact tttattccac agccaaactc
    68941 acaatatact ccgttaatat ttatcacttc attaacaact gaaaccttaa ttaaacatta
    69001 gccagtccgg gtaactcact attcgaatta tattttcgct gcgatataac cttgagccac
    69061 atcaacattg agtcagatta ttattcaaac caacattcgt acacatttta agtattgctg
    69121 atagaaacca ttctcattat cattgtgttg ttgattattt aatctctcct ttgttggcaa
    69181 atcatctggt ctcatgtcgc ggtcaaacgc cccatgaggt agttatccag ttaatgagaa
    69241 acaagtaggc acctatgcaa tacactccag atactgcgtg gaaaatcact ggcttttccc
    69301 gtgaaatcag cccggcatat cgccaaaaac tgctttctct aggcatgtta cctggctcct
    69361 cttttgatgt ggtgcgcgtc gctccactcg gcgaccccat tcatatcgaa acccgtcgtg
    69421 tgagcctggt attacgcaaa aaagatctgg ccttattaga agtggaagcg gtttcctgtt
    69481 aatacggtga taacaacaat gaaaaaatta accattggct taattggtaa tccaaattct
    69541 ggcaagacaa ccttatttaa ccagctcact ggcgcacgtc agcgtgtagg taactgggct
    69601 ggcgttaccg tcgaacgtaa agaagggcaa ttctccacca ccgatcatca ggtcacgctg
    69661 gtggacctgc ccggcaccta ttctctgacc accatctcat cgcagacctc gctcgatgag
    69721 caaatcgcct gtcactacat tttgagtggc gacgccgacc tgctgattaa cgtggtggat
    69781 gcgtctaacc ttgagcgtaa cctgtacctg acgctgcaac tgctggaact cggcattccc
    69841 tgcatcgtgg cgctgaacat gctcgacatt gccgagaagc aaaatattcg tattgaaatt
    69901 gatgctctat cggcacgtct tggctgcccg gtgatcccgc tggtttcaac ccgtggtcgc
    69961 ggtattgaag cgctcaagct ggcgattgat cgctataaag ctaacgagaa tgtggaactg
    70021 gtgcattacg cacagccgct actcaacgaa gcagattcac tggcaaaagt gatgccttcc
    70081 gacatcccgc tgaaacaacg tcgctggctg ggcctgcaaa tgctggaagg cgatatctac
    70141 agccgcgcct acgccggtga agcgtcgcag catctggatg ccaccctcgc ccgtctgcgt
    70201 aatgaaatgg atgatccggc gctgcacatt gccgatgcgc gttaccagtg cattgctgcc
    70261 atctgtgatg tggtaagcaa caccctgacg gcagaaccca gccgtttcac cactgcggta
    70321 gataaaatcg tgctcaaccg tttcctcggt ctgccgattt tcctctttgt gatgtacctg
    70381 atgttcctgc tggctatcaa catcggcggg gcgttacagc cgctgtttga cgtcggctcc
    70441 gtggcgctat ttgtgcatgg tattcaatgg attggctaca cgctccactt cccggactgg
    70501 ctgactatct tcctcgccca gggcatgggt ggcggtatta acaccgtgct gccactggtg
    70561 ccgcagatcg gcatgatgta cctgttcctc tccttccttg aggactccgg ctatatggcg
    70621 cgtgcagcgt ttgtgatgga ccgcctgatg caggcgctgg gcttgccggg gaaatccttt
    70681 gtgccgctga tcgtcggttt cggttgtaac gtaccgtcgg taatgggcgc acgtacgctt
    70741 gatgcaccgc gtgaacgtct gatgaccatc atgatggcac cgtttatgtc ctgcggcgcg
    70801 cgtctggcta tcttcgcagt attcgcggct gccttcttcg ggcagaacgg tgccctggcg
    70861 gttttctcgc tgtatatgct cggtatcgtg atggcggtgc tgactggcct gatgctcaag
    70921 tacaccatca tgcgcggtga agcaacgccg tttgtcatgg agctgccggt ataccatgta
    70981 ccacacgtta aaagcctgat tatccagacc tggcagcgtc tgaagggctt cgttctgcgt
    71041 gctggtaaag tgattatcat cgtcagcatt ttcctgagcg ctttcaacag cttctcgttg
    71101 agcgggaaaa tcgtcgataa catcaacgac tcggcgctgg cttccgtcag ccgggtgata
    71161 acccctgtct tcaagccgat tggcgtacat gaagataact ggcaggcaac ggttggcctg
    71221 tttacgggcg caatggcaaa agaagtggtg gtgggtacgc tcaacaccct ctacaccgca
    71281 gaaaatattc aggacgaaga gttcaatccg gcggaattca acctcggtga agagctgttc
    71341 agtgcggtag atgaaacctg gcagagcctg aaagacacct tcagccttag cgtactgatg
    71401 aaccccattg aagctagcaa aggcgacggt gaaatgggta ccggggcgat gggcgtgatg
    71461 gatcagaaat tcggtagcgc agctgccgca tacagctacc tgattttcgt cctgctgtat
    71521 gtaccatgta tctcggtgat gggagccatc gcccgtgaat caagccgtgg ctggatgggc
    71581 ttctccatcc tgtgggggct gaatatcgct tactcactgg caacattgtt ctatcaggtc
    71641 gccagctaca gtcagcaccc aacttacagc ctggtgtgca ttctggcggt aatcctgttt
    71701 aacatcgtag ttatcggtct gctgcgccgc gcgcgtagcc gggtggatat cgaactgctg
    71761 gcaacccgca agtcggtaag cagttgctgc gcagccagca ccaccggtga ttgccattaa
    71821 tggcttcact tattcaggta cgtgatttgc tggcgttacg gggccgtatg gaagcgaccc
    71881 agataagcca gacattgaac actccgcagc caatgattaa cgccatgctg caacagctgg
    71941 aaagcatggg caaagccgtg cggattcagg aagaacctga cggctgcctc tctggcagtt
    72001 gtaaaagctg cccggaaggc aaagcctgtc tgcgcgagtg gtgggcgctg cgttaaccat
    72061 actccatcgc ctgttttgaa aagcccggta tgcgtttgca tccgggcttt tttgcgtgcg
    72121 gctttccata aaaatgcaat tcttgcagcg caacgtaagt tcctctgaaa gcgtctcgca
    72181 gggatgaaaa ctcgctaatg cacagatgtg gagtggcgcg tagagtcgcg gcattcaaac
    72241 aacaggtgaa ggaacgccat gagtaaaaag cagagttcca ccccacatga tgcgctgttc
    72301 aaactctttt tacgccaacc ggagacggca cgcgattttc ttgcgtttca tttaccggca
    72361 cccattcacg cgctctgtga tatgaaaacc ctcaagctgg agtcgagcag ctttattgat
    72421 gacgatctgc gtgaaagcta ttccgatgtg ctgtggtcgg tgaaaacgga acaaggacca
    72481 ggatacatct attgtctgat tgaacatcaa agcacctcaa acaaactgat cgcatttcgc
    72541 atgatgcgtt acgctattgc cgcaatgcaa aatcaccttg atgctggata caaaacgttg
    72601 ccgatggtgg tgccattgtt gttttaccac ggtattgaaa gcccctatcc ctattcgctg
    72661 tgttggctgg attgtttcgc cgatcccaaa ctggcaaggc agctttatgc ctccgcattt
    72721 ccgctgattg atgtcaccgt catgcctgat gatgaaatca tgcagcaccg acgtatggcg
    72781 ctgctggagt taattcaaaa acatattcgt caacgcgatc tgatggggct ggtagagcaa
    72841 atggcctgct tattaagtag tggatacgct aatgacagac aaatcaaagg gctgtttaat
    72901 tacatactgc aaactggcga cgctgtacgt tttaacgatt ttatcgacgg cgttgccgaa
    72961 cgttcaccga aacacaagga gagtttaatg actattgcgg aaagattgcg gcaggagggg
    73021 gaacaatcca aagccctgca tatagccaaa ataatgcttg aatccggagt ccctcttgca
    73081 gacatcatgc gatttaccgg gctgtcagaa gaagagttgg ctgcggcgag tcagtagata
    73141 ctctaccctg cttaagtgct actttgtgct atcttgcatg taacacaaag tagcacttag
    73201 ggaactgata tgcagacagt gaaattacgg caacaaggag gagccatgat cgttactatc
    73261 ccacgagatc ttgccatcga tcttggctgg tcaccgggca cagagcttac cgtggagaaa
    73321 aaaggcgata gcgttaattt gcgcgccaca gagcacaaac cgcgtgggcg tcttaccgtc
    73381 gcgcagttgt tgagccaaat cgatgaaagc gaaattacgg agctaaatca gtccacagaa
    73441 ggatgggctg aagggaaaaa aggtaatgaa gcatggtaaa ggcacggacg ccacatcgtg
    73501 gtgagatctg gtattttaac cctgatccgg ttgccgggca tgaacttcag gggccacatt
    73561 attgcattgt ggtaacggac aaaaaactca acaatgtttt aaaagttgct atgtgctgcc
    73621 cgatttcaac aggggcaaat gcagcacgtt ccacaggggt gacggtgaac gtcctcccct
    73681 gtgatacgca aaccggtaac ctgcatggcg ttgtactttg tcaccagcta aaagccgtcg
    73741 atcttattgc ccgtggcgct aaatttcata ccgttgccga tgaaaaattg attagtgaag
    73801 ttatcagtaa actggtgaat ttaatcgacc cacaataatt agctctcact taccaccaac
    73861 accctctgct tcaacgccac cagcaggtga caaaactcga ccggatgcga aataaacggc
    73921 gcatgggccg ctttggcgaa gatatatgat tcgctgtgag gccaaagttt atccagcatc
    73981 ggcaccactt tgcgcggcac cagaccgtcg agatagccat acaatcgcaa aaacggcatg
    74041 gacacgtttt gcagcggctg acggagatcg accgttttca ggatttccag cccgccatta
    74101 agcacgtcaa cctccggcat cggtaacgcc agaacggttt tcttcaacgc ccgcgcatcc
    74161 tggcgcgccg tttcagtccc catggtttgt aacgccagga accgctccac tgtacgctga
    74221 aaatcatcac tgagttgctg ctgaaatccc gccagcacgt ccggttttat ccccggccac
    74281 tcgtcacgag cactaaaata aggtgacgac gccacggtga ccagcgcctg aacacgctcg
    74341 ggatgggtta acgcaatctg gcttgccacc agcccgccca gactccagcc taaccaaatg
    74401 gctttatcag gtgcctgttg cagcacggct tcggccatat cagcaagtga cagcgcacca
    74461 aatccccggc tacgcccgaa gccgggcagg tcaacaaggt gcagcgtaaa atgcgagcta
    74521 agttcctcgt caatgcaacg ccacacttcg gcattcagtc cccatccgtg cagcagcaca
    74581 agatgaacat tcccctgacc tttggtctgc caccagatgt tattcatccg ctattgttct
    74641 cttttgactt acaaggatga acatatgcta acagtaccgg gattatgctg gctatgccga
    74701 atgccactgg cgttaggtca ttgggggatt tgttcggtct gttcacgcgc cacccgcaca
    74761 gataaaacgt tatgcccaca atgtggatta cccgccacac actcccatct tccctgcggt
    74821 cgctgcctgc aaaaaccgcc gccctggcaa agactggtca cggttgccga ctatgcgccg
    74881 ccgttaagtc cgcttatcca ccagcttaaa ttttcccggc gcagtgaaat cgccagcgcc
    74941 ctgtcacgtc tgttactatt ggaagtctta cacgctcgtc gcaccaccgg gttgcaattg
    75001 ccggatcgca tcatcagcgt tccgttatgg cagcggcgtc actggcgtcg gggatttaat
    75061 cagagcgatt tgctgtgtca gccgttatca cgctggttgc actgccaatg ggatagcgaa
    75121 gccgtcacac gtacacgggc cactgcgacc cagcattttc tcagtgcccg gctgcgcaag
    75181 cgcaacctga aaaatgcctt tcgtcttgaa ttgcccgtgc aaggtcgcca tatggtgatt
    75241 gtggatgatg tcgttaccac cggaagtacc gtcgcagaga ttgcgcagtt gcttttacgc
    75301 aatggtgcgg cgactgtcca ggtctggtgc ctttgtcgaa ccttgtagag cctcgatgat
    75361 gggcgtatta taaccaacta aaatagtcaa ctattaggcc attactatga tccgtatttc
    75421 cgatgctgca caagcgcact ttgccaaact gctggcaaat caggaagaag ggacacaaat
    75481 ccgcgtattt gtgattaacc ctggcacgcc taacgctgaa tgtggtgttt cttattgtcc
    75541 gccggatgcc gtggaagcca ccgacactgc cctgaaattt gacctgctga ccgcgtatgt
    75601 tgatgagtta agcgcaccat acctggaaga tgcagagatc gactttgtta ccgaccagtt
    75661 gggttctcag ttaacgctga aagccccgaa cgccaaaatg cgtaaagtgg cagacgatgc
    75721 accgctgatg gagcgcgtgg agtatatgct gcagtcgcag atcaacccac agcttgctgg
    75781 tcacggtggt cgcgtttcgc tgatggaaat caccgaagac ggttacgcca ttctgcaatt
    75841 tggcggcggc tgtaacggtt gttccatggt cgatgtgacg ctgaaagaag ggatcgagaa
    75901 gcagctgctg aacgaattcc cggagctgaa aggtgtgcgc gatctcaccg aacaccagcg
    75961 cggcgaacac tcctactact aagttatccc ctcttttgta gattgcccga tgcgacgcta
    76021 aagcgtcatt tcgggcctac gttgaacatg tgccagacgt tggcacagca ttgctttggc
    76081 aaatccctcc cttcgtttcc ccgcgataat atgaccaacc tctcataatt taaatttacc
    76141 ccgctctggt gattctcaaa cgccagatgt tacccgtatc attcacatgg gtaccaaaca
    76201 tactcctgac atctgactac aataattagt tttagtgggt atcagtcgtg gtgccgcaat
    76261 atctctgttc ccgattggga taattagagt ttgtcgtcag aaaattgaca ttacccataa
    76321 caaatgaaag gccaggtaaa tcatgccatt agtcattgtt gctatcggtg taatcttgtt
    76381 gttgctcctg atgatccgct tcaaaatgaa cggcttcatc gctctcgtcc tcgtggcgct
    76441 tgctgttgga ttaatgcaag gaatgccgct ggataaagtt attggctcca tcaaagccgg
    76501 tgtcggcggg acgctcggta gccttgccct gatcatgggt tttggcgcaa tgctggcaaa
    76561 ctgcggtggc gcacaacgta tcgccaccac gctgattgcc aaatttggta aaaagcacat
    76621 ccagtgggcg gtggtgttaa ccggttttac cgttggtttt gccctgttct atgaagtggg
    76681 cttcgtgctg atgctgccgc tggtgtttac catcgcggct tccgcgaata ttccattgct
    76741 gtatgttggt gtaccaatgg cggctgcact gtctgtgacc cacggcttcc tgccaccgca
    76801 tccgggcccg actgcgattg ccaccatttt caatgccgat atgggtaaaa ccctgctgta
    76861 cggtactatt ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg ctcgcgtgtt
    76921 gaaaggtatc gataagccaa tcccggaagg tctctacagc gcgaaaacct tcagcgaaga
    76981 agagatgccg agctttggcg tcagcgtctg gacctctctg gtgccggttg tactgatggc
    77041 gatgcgtgcg attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc cggtagcgga
    77101 gttcctcggt gacccggtaa tggcaacgct gattgccgtg ctgattgcga tgttcacctt
    77161 tggtctgaac cgtggtcgtt caatggatca gattaacgac acgctggttt cttccatcaa
    77221 aatcattgcg atgatgctgt tgatcatcgg tggtggcggt gcgttcaagc aggtgctggt
    77281 agacagcggc gtggacaaat acattgcttc catgatgcac gaaaccaaca tttctccgct
    77341 gctgatggcc tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg caaccgttgc
    77401 ggcaatcact gcgggtggta tcgcggcacc gctgattgca acgacgggtg ttagcccaga
    77461 gctgatggtt attgcggtgg gttccggtag tgtgattttc tctcatgtga acgatccggg
    77521 cttctggctg ttcaaagagt actttaacct gactatcggc gagaccatca aatcctggtc
    77581 gatgctggaa accattatct cggtgtgcgg tctggtaggc tgtctgctgc tgaatatggt
    77641 gatttgataa cactgactgc cggatgcatc gcatccgaca tcaaccgcac tctacttctt
    77701 cttcgctgca gctctgcgcc gtctgtccaa atccttcagc aacttgttca cgccatcatc
    77761 ggcaaacatc gactcaagcg ttgcggaaag cttgcgtcgc cagtttttat actggtaact
    77821 ggtgccagga atattcaccg gttcggccat atccagccag tcttccggct gtagtcctaa
    77881 cagagcactg ttactgtcgg caatgtagcg ctgcaaacca cggttcagcg tcgtcgtcat
    77941 cgacatcaac gatgccttat gcccggcacg ttttggcaga caaccatatt tatgcagtgc
    78001 atccagcagc ccttgcttcg ccagttcgcg atcctgatac agaccgcgca gtaccacttc
    78061 atccggatac agccccaggg ttttgcccag cgttagatcc ccgctctccc agtaaccacg
    78121 cagcgttggc aggtcatgtg tcgccgcaac cgccatcgac tgctccggat acgctttcgg
    78181 tgcacggaac gtcttctcgt ggtcgttttc gaaatagagc actttgtaag agtacacacc
    78241 gctgctgcgc agcttaccga caatctctac cggtacggta ccgagatctt caccaatcac
    78301 catacagcga tgacgtttac tttcgagtgc cagaatcgag agcagatcat ccaccggata
    78361 gtgaacatac gcgccctgat ctgccgtctc gccatacggt atccaccaca aacgcagcat
    78421 cgacatcaca tggtcaattc gtaatgcgcc gcagttttgc atattggcac gcaacagctc
    78481 gataaacggt tcataggcac gcgcggtgat gatatgcggg tccattggcg gtaatcccca
    78541 gttctgcccc aacgggccga ggatatccgg cggcgcgcca accgatgctt tcaggcaata
    78601 tagttcacgg tcacaccagg tttccgcccc accttccgct acgccaaccg ccagatcacg
    78661 atacaagcca atcggcattt catagccctg gcttatctcc cagcaggcgg caaactggct
    78721 gtaagccagc cactgcaacc agagataaaa atcgacgtca tcacgatgtt cttcgcagaa
    78781 ctgacgcact tctggtgaat ccacgttctt atacatctct ggccatgcag gccagcccca
    78841 gcgcatttcg tcctctttca cttgctgggc atgtagcgca tcaaaggctg cctgccagaa
    78901 caggctgtcg ccctgctctg caacaaactg gcgaaacgcg gccatctgct catcatcacg
    78961 ttgcgcgaaa cctttccacg ccattcgtaa tgctgtcatt tttagggcgg taaccgtgga
    79021 gtaatcgacc cagtcggcat cgcgcgcctg ttgcagcgtc tgttgcgtgg tcggcaactg
    79081 ccaccaggcc tgagcctctt cgctaagatg gaaatcttca acggcgttaa cgtcgatata
    79141 aatcacattc agccaacggc gagaagacgg gctgtatggg ctggcgctct ccggatttgc
    79201 cggatagagc gcatgaatcg ggttcaggcc aatgaacgac ccgccacgtt ttgccacatc
    79261 caccagcatc gctttgagat cgccaaaatc cccaataccc cagttttttt ccgatcgcag
    79321 cgtataaagc tgaacgcagg caccccacag cttttgttta ttcagcaaag cctgcggttc
    79381 gtaacaacgt ttcggggcga caatcacccg gcaatgcgcg cgctggtcgt cctgggtgag
    79441 tgttagcgtg tgataacctt ccggcagctt cgtcggtaga ttgaacgctt tgccccccgt
    79501 tacatggcct ttgtactgcg ttccttcttc ggtggtcagc tgccagctat attcgccgct
    79561 gccctccacc accatcggca tttttttgcc gctggtataa accatgacat tcgggactgg
    79621 cgttaccgcc actttcgtgg cggtacgttg atgcatcgcg tcaagcaaac gccgtttggt
    79681 ttcggcgcta atcgactgcg gtttaccgtg ggcattgatg taattggggc taatccccgc
    79741 cgccagcgcg gcattatcca gacgtttgct ttccatcgag cttccttagc gttttgcctg
    79801 ccagatacga gcctgataat cgcgaataga gcgatccgag ctaaacatac cgcagcgggc
    79861 ggtattgagg atcgccgcgc gagtccaggc ctcctggtcg cggtacagca catccacctg
    79921 cttttgtgcc tcaacatagg ctgcgaaatc cgccatcacc agatacggat cgccgccctg
    79981 tttgccgata ctgtgcagca tctggtcgaa ggcatgctta tcgccgtcgc tgtatttacc
    80041 gctttccagc tctttcaata ctgcgtccag caccttatct ttcttccgcc atttcaccgg
    80101 gtcgtagcct ttggccagaa tagccttcac ttgttccacg gtatgaccaa aaataaagat
    80161 attttcttca ccgactttct cggcgatttc aacgttcgca ccatccagcg taccgacagt
    80221 aagcgcacca ttgagcgcca gtttcatatt gccggtaccg gaagcttctt tacctgcagt
    80281 cgaaatttgt tcggagatat ccgccgccgg gatcagtttt tccgccgccg aaacgcaata
    80341 atccggcagg aacaccacct tcaacttatc gccaaccagt ggatcgttgt tgatcacgtc
    80401 agccactttg ttgatcgcaa agataatatt cttcgccagg tagtagcccg gtgccgcttt
    80461 cgcgccgaag aggaagacgc gcggtacgcg atcagcctgc aggttttcac gaatttcttt
    80521 gtacaacgcc agaatatgca gcagattcag gtgctggcgt ttgtactcat gcagacgttt
    80581 gatctgaata tcgaaaatcg cctgtggatt gatctcaata ccggtacgaa ctttcacaaa
    80641 ctccgccaga cggactttat tcgcctgctt gatctcgcga tattgctgac ggaatttcgc
    80701 atcatcagcg aatttttcca gattgatcag ctgatcgaga tcgttagccc actctttttg
    80761 cagtgattta tccaacagag ccgccagtgc agggttgcac tgtttgatcc agcgacgtgg
    80821 ggtaataccg ttggtgacgt tatggaattt gttcggccat agctggtgat attccgggaa
    80881 cagatctttc accaccagat ccgagtgcag cgccgcaaca ccgttcaccg cgaaaccgcc
    80941 aaccacacac aggttcgcca tatgcacttg tttatcgtgc accaccgcca gtttggccca
    81001 cactttttca tcgcccggcc aggttttctc caccagcgtt ttaaagcgag tattaatttc
    81061 gttaataatc tgcatatggc gcggcagtaa gcctttcacc agtttcacat cccagcgttc
    81121 cagcgcttct ggcatcaggg tatggttggt gtaagcgaaa gttttgctgg tgatggccca
    81181 ggcgtcatcc cagctcatct ggtgctcatc ggtcagcacg cgcagcagtt ctggaatcgc
    81241 gatagttgga tgggtatcgt tcagctgaat aacttcgtaa tccgccagtt cgtgcagttt
    81301 acgccccgcc agatgatggc gacgcaaaat atccgctacc gaacaggcac actggaagta
    81361 ttgctgcatc aggcgcagct ttttaccggc agtatggttg tcgtttggat agagaacttt
    81421 ggtcagtttt tccgcattga tgccttgctg ttcggcgcgc aggaaatcac cgtcgttaaa
    81481 tttagttaga tcaaacggat gcgcatgcgt cgcctgccac agacgcagcg gctgcgccac
    81541 gccattacga tagccgacaa cggggagatc ccacgcttga ccggtaatgg taaactccgg
    81601 ctcccagcgt ccgtctttca tcactttacc gccaatccct acctgcacat ccagtgcttc
    81661 gttgtggcgg aaccacgggt agttactgcg atgccagtca tccggcgctt caacctgttt
    81721 gccatcgaca aaagactggc ggaacaaacc atattgatag ttcagaccgt aacccgtcgc
    81781 agactgaccg acagttgcca ttgagtcgag gaagcacgcc gccagacgtc ccagaccacc
    81841 gttacccagc gccgggtcga tctcttcttc cagcaggtca gtcaggttga tgtcataagc
    81901 cttcaacgaa tcctgtacat cctgatacca gccgagattc aacaggttgt tgcccgtcag
    81961 acgaccaatc aaaaactcca ttgagatgta gttaacatgt cgctgattcg ccaccggttt
    82021 ggcgaatggc tgagcacgca gcatttcggc cagtgcttca ctcactgcca gccaccactg
    82081 gcgaggagtc atttcagccg cagaatttaa gccataacgc tgccactgac gtgaaagcgc
    82141 ttcctgaaat tgcttatcgt taaaaatagg ttgtgacata ggagttccac ttttcttaga
    82201 ttttcaacac aacgttatcg ctagtttgcc aggctcgatg ttgaccttcc tcatcctgcg
    82261 ggggattagg cagggaggag ttgcggggat gagcaaggaa atgtgatctc gaccacttaa
    82321 agctagtgca aaccacagga ttagcagcaa atcaatgcca tacagcgcag aaaatctgta
    82381 tctaagtgca aaaaatggcc gttgcgtatt ttcaaaaagc ggaaggtaac tctataaatt
    82441 aagtaaagga gtgaaacagt ttcacaagta aaatatccag tgtgctccat ctcattctta
    82501 atagatttat taagatcatc tttttagatg gcactttcat caggaatgaa gaagaaaccc
    82561 ttgcttaaat gaatctgat
//
LOCUS       DAJTNM010000005        69007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTNM010000005 DAJTNM010000000
VERSION     DAJTNM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.005"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..190
                     /locus_tag="OO172_000419"
     CDS             <1..190
                     /locus_tag="OO172_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5510724.1"
                     /translation="VGIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGG
                     ELETGKFQGTATITVMVR"
     gene            complement(233..1093)
                     /gene="rsmI"
                     /locus_tag="OO172_000420"
     CDS             complement(233..1093)
                     /gene="rsmI"
                     /locus_tag="OO172_000420"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5510725.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLTADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1158..3194
                     /gene="lpoA"
                     /locus_tag="OO172_000421"
     CDS             1158..3194
                     /gene="lpoA"
                     /locus_tag="OO172_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5510726.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLALQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3152..3547
                     /locus_tag="OO172_000422"
     CDS             3152..3547
                     /locus_tag="OO172_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5510727.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3567..4157
                     /gene="diaA"
                     /locus_tag="OO172_000423"
     CDS             3567..4157
                     /gene="diaA"
                     /locus_tag="OO172_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5510728.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4167..4742
                     /gene="yraP"
                     /locus_tag="OO172_000424"
     CDS             4167..4742
                     /gene="yraP"
                     /locus_tag="OO172_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5510729.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4856..5896)
                     /locus_tag="OO172_000425"
     CDS             complement(4856..5896)
                     /locus_tag="OO172_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5510730.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLPGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5969..6604)
                     /locus_tag="OO172_000426"
     CDS             complement(5969..6604)
                     /locus_tag="OO172_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5510731.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6732..7250
                     /gene="yhbO"
                     /locus_tag="OO172_000427"
     CDS             6732..7250
                     /gene="yhbO"
                     /locus_tag="OO172_000427"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5510732.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7230..7673)
                     /locus_tag="OO172_000428"
     CDS             complement(7230..7673)
                     /locus_tag="OO172_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5510733.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7724..8026
                     /gene="yhbQ"
                     /locus_tag="OO172_000429"
     CDS             7724..8026
                     /gene="yhbQ"
                     /locus_tag="OO172_000429"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5510734.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8013..8516)
                     /locus_tag="OO172_000430"
     CDS             complement(8013..8516)
                     /locus_tag="OO172_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5510735.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8510..9034)
                     /locus_tag="OO172_000431"
     CDS             complement(8510..9034)
                     /locus_tag="OO172_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5510736.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVCDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9243..10238
                     /locus_tag="OO172_000432"
     CDS             9243..10238
                     /locus_tag="OO172_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5510737.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10247..11125
                     /locus_tag="OO172_000433"
     CDS             10247..11125
                     /locus_tag="OO172_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5510738.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11206..12213
                     /locus_tag="OO172_000434"
     CDS             11206..12213
                     /locus_tag="OO172_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5510739.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12331..13575)
                     /gene="mtr"
                     /locus_tag="OO172_000435"
     CDS             complement(12331..13575)
                     /gene="mtr"
                     /locus_tag="OO172_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5510740.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13729..15618)
                     /gene="deaD"
                     /locus_tag="OO172_000436"
     CDS             complement(13729..15618)
                     /gene="deaD"
                     /locus_tag="OO172_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5510741.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15611..15691)
                     /gene="yrbN"
                     /locus_tag="OO172_000437"
     CDS             complement(15611..15691)
                     /gene="yrbN"
                     /locus_tag="OO172_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5510742.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15798..16682)
                     /gene="nlpI"
                     /locus_tag="OO172_000438"
     CDS             complement(15798..16682)
                     /gene="nlpI"
                     /locus_tag="OO172_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5510743.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEHTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16791..18926)
                     /gene="pnp"
                     /locus_tag="OO172_000439"
     CDS             complement(16791..18926)
                     /gene="pnp"
                     /locus_tag="OO172_000439"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5510744.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19173..19442)
                     /gene="rpsO"
                     /locus_tag="OO172_000440"
     CDS             complement(19173..19442)
                     /gene="rpsO"
                     /locus_tag="OO172_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5510745.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19591..20535)
                     /gene="truB"
                     /locus_tag="OO172_000441"
     CDS             complement(19591..20535)
                     /gene="truB"
                     /locus_tag="OO172_000441"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5510746.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20535..20936)
                     /gene="rbfA"
                     /locus_tag="OO172_000442"
     CDS             complement(20535..20936)
                     /gene="rbfA"
                     /locus_tag="OO172_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5510747.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21100..23748)
                     /gene="infB"
                     /locus_tag="OO172_000443"
     CDS             complement(21100..23748)
                     /gene="infB"
                     /locus_tag="OO172_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5510748.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23773..25260)
                     /gene="nusA"
                     /locus_tag="OO172_000444"
     CDS             complement(23773..25260)
                     /gene="nusA"
                     /locus_tag="OO172_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5510749.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25288..25740)
                     /gene="rimP"
                     /locus_tag="OO172_000445"
     CDS             complement(25288..25740)
                     /gene="rimP"
                     /locus_tag="OO172_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5510750.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25947..26023)
                     /locus_tag="OO172_000446"
     tRNA            complement(25947..26023)
                     /locus_tag="OO172_000446"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25987..25989),aa:Met,seq:cat)
     gene            26371..27714
                     /gene="argG"
                     /locus_tag="OO172_000447"
     CDS             26371..27714
                     /gene="argG"
                     /locus_tag="OO172_000447"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5510751.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27722..29347)
                     /locus_tag="OO172_000448"
                     /pseudo
     CDS             complement(27722..29347)
                     /locus_tag="OO172_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(29806..29892)
                     /locus_tag="OO172_000449"
     tRNA            complement(29806..29892)
                     /locus_tag="OO172_000449"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29856..29858),aa:Leu,seq:gag)
     gene            complement(29907..30239)
                     /gene="secG"
                     /locus_tag="OO172_000450"
     CDS             complement(29907..30239)
                     /gene="secG"
                     /locus_tag="OO172_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5510752.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30467..31804)
                     /gene="glmM"
                     /locus_tag="OO172_000451"
     CDS             complement(30467..31804)
                     /gene="glmM"
                     /locus_tag="OO172_000451"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5510753.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRVLQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31797..32645)
                     /gene="folP"
                     /locus_tag="OO172_000452"
     CDS             complement(31797..32645)
                     /gene="folP"
                     /locus_tag="OO172_000452"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5510754.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32735..34669)
                     /gene="ftsH"
                     /locus_tag="OO172_000453"
     CDS             complement(32735..34669)
                     /gene="ftsH"
                     /locus_tag="OO172_000453"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5510755.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34769..35398)
                     /gene="rlmE"
                     /locus_tag="OO172_000454"
     CDS             complement(34769..35398)
                     /gene="rlmE"
                     /locus_tag="OO172_000454"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5510756.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35524..35817
                     /gene="yhbY"
                     /locus_tag="OO172_000455"
     CDS             35524..35817
                     /gene="yhbY"
                     /locus_tag="OO172_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5510757.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKKRKISLPR"
     gene            complement(35973..36449)
                     /gene="greA"
                     /locus_tag="OO172_000456"
     CDS             complement(35973..36449)
                     /gene="greA"
                     /locus_tag="OO172_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5510758.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36697..38130
                     /gene="dacB"
                     /locus_tag="OO172_000457"
     CDS             36697..38130
                     /gene="dacB"
                     /locus_tag="OO172_000457"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5510759.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38316..39488)
                     /gene="cgtA"
                     /locus_tag="OO172_000458"
     CDS             complement(38316..39488)
                     /gene="cgtA"
                     /locus_tag="OO172_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5510760.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39504..40469)
                     /locus_tag="OO172_000459"
     CDS             complement(39504..40469)
                     /locus_tag="OO172_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5510761.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40596..40853)
                     /gene="rpmA"
                     /locus_tag="OO172_000460"
     CDS             complement(40596..40853)
                     /gene="rpmA"
                     /locus_tag="OO172_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5510762.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40874..41185)
                     /gene="rplU"
                     /locus_tag="OO172_000461"
     CDS             complement(40874..41185)
                     /gene="rplU"
                     /locus_tag="OO172_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5510763.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41444..42415
                     /gene="ispB"
                     /locus_tag="OO172_000462"
     CDS             41444..42415
                     /gene="ispB"
                     /locus_tag="OO172_000462"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5510764.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42643..42921
                     /gene="sfsB"
                     /locus_tag="OO172_000463"
     CDS             42643..42921
                     /gene="sfsB"
                     /locus_tag="OO172_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5510765.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(42969..44228)
                     /gene="murA"
                     /locus_tag="OO172_000464"
     CDS             complement(42969..44228)
                     /gene="murA"
                     /locus_tag="OO172_000464"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5510766.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44283..44537)
                     /gene="ibaG"
                     /locus_tag="OO172_000465"
     CDS             complement(44283..44537)
                     /gene="ibaG"
                     /locus_tag="OO172_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5510767.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44697..44990)
                     /gene="mlaB"
                     /locus_tag="OO172_000466"
     CDS             complement(44697..44990)
                     /gene="mlaB"
                     /locus_tag="OO172_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5510768.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44990..45625)
                     /gene="mlaC"
                     /locus_tag="OO172_000467"
     CDS             complement(44990..45625)
                     /gene="mlaC"
                     /locus_tag="OO172_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5510769.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45644..46195)
                     /gene="mlaD"
                     /locus_tag="OO172_000468"
     CDS             complement(45644..46195)
                     /gene="mlaD"
                     /locus_tag="OO172_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5510770.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46200..46982)
                     /gene="mlaE"
                     /locus_tag="OO172_000469"
     CDS             complement(46200..46982)
                     /gene="mlaE"
                     /locus_tag="OO172_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5510771.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46990..47799)
                     /gene="mlaF"
                     /locus_tag="OO172_000470"
     CDS             complement(46990..47799)
                     /gene="mlaF"
                     /locus_tag="OO172_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5510772.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48009..48974
                     /locus_tag="OO172_000471"
     CDS             48009..48974
                     /locus_tag="OO172_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5510773.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48988..49974
                     /gene="kdsD"
                     /locus_tag="OO172_000472"
     CDS             48988..49974
                     /gene="kdsD"
                     /locus_tag="OO172_000472"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5510774.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49995..50561
                     /gene="kdsC"
                     /locus_tag="OO172_000473"
     CDS             49995..50561
                     /gene="kdsC"
                     /locus_tag="OO172_000473"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5510775.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50558..51133
                     /gene="lptC"
                     /locus_tag="OO172_000474"
     CDS             50558..51133
                     /gene="lptC"
                     /locus_tag="OO172_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5510776.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51102..51659
                     /gene="lptA"
                     /locus_tag="OO172_000475"
     CDS             51102..51659
                     /gene="lptA"
                     /locus_tag="OO172_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5510777.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51666..52391
                     /gene="lptB"
                     /locus_tag="OO172_000476"
     CDS             51666..52391
                     /gene="lptB"
                     /locus_tag="OO172_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5510778.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52439..53872
                     /gene="rpoN"
                     /locus_tag="OO172_000477"
     CDS             52439..53872
                     /gene="rpoN"
                     /locus_tag="OO172_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5510779.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53895..54182
                     /gene="hpf"
                     /locus_tag="OO172_000478"
     CDS             53895..54182
                     /gene="hpf"
                     /locus_tag="OO172_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5510780.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54300..54791
                     /gene="ptsN"
                     /locus_tag="OO172_000479"
     CDS             54300..54791
                     /gene="ptsN"
                     /locus_tag="OO172_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5510781.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54837..55691
                     /gene="rapZ"
                     /locus_tag="OO172_000480"
     CDS             54837..55691
                     /gene="rapZ"
                     /locus_tag="OO172_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5510782.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55688..55960
                     /gene="npr"
                     /locus_tag="OO172_000481"
     CDS             55688..55960
                     /gene="npr"
                     /locus_tag="OO172_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5510783.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56173..56805
                     /gene="yrbL"
                     /locus_tag="OO172_000482"
     CDS             56173..56805
                     /gene="yrbL"
                     /locus_tag="OO172_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5510784.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56802..57530)
                     /gene="mtgA"
                     /locus_tag="OO172_000483"
     CDS             complement(56802..57530)
                     /gene="mtgA"
                     /locus_tag="OO172_000483"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR5510785.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57527..58180)
                     /gene="elbB"
                     /locus_tag="OO172_000484"
     CDS             complement(57527..58180)
                     /gene="elbB"
                     /locus_tag="OO172_000484"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5510786.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58410..60746)
                     /gene="arcB"
                     /locus_tag="OO172_000485"
     CDS             complement(58410..60746)
                     /gene="arcB"
                     /locus_tag="OO172_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5510787.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEKSTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60842..61771)
                     /locus_tag="OO172_000486"
     CDS             complement(60842..61771)
                     /locus_tag="OO172_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5510788.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62446..66906
                     /gene="gltB"
                     /locus_tag="OO172_000487"
     CDS             62446..66906
                     /gene="gltB"
                     /locus_tag="OO172_000487"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5510789.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPTTTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66919..68337
                     /gene="gltD"
                     /locus_tag="OO172_000488"
     CDS             66919..68337
                     /gene="gltD"
                     /locus_tag="OO172_000488"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5510790.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68521..69000
                     /locus_tag="OO172_000489"
     CDS             68521..69000
                     /locus_tag="OO172_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5510791.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        16854 a        18751 c        17148 g        16254 t
ORIGIN      
        1 tgtcggtatt cgtattgaaa atctcggcgg cggagttgca aatattcctt ttcagaacgg
       61 tatccttccc gttgatcctt ccgggcatgg caccgtcaat atgcgcgcct ggccagttaa
      121 tctggtcggt ggtgagctgg aaacaggaaa atttcagggc acagccacca ttaccgtcat
      181 ggtgcggtaa aagaagacgt tgcgttttga ttaacgcaac gcttcggcac tgttacccct
      241 gctgctccag cgcatactta tacagcgcat ttttcttcac gccatgaatt tctgcggcca
      301 gcgccgccgc ttttttcagc ggcagttctg cctgtagcag cgccagcgtg cgcagggcat
      361 cggcggttaa gtcttcttcc tgtgctttat gaccttcgac aatcagcacc atttcgcctt
      421 tgcgacggtt ttcatcttcc tttacccacg ccagcagctc gccaacgggc gcgccgtgaa
      481 tggtttccca ggttttggtc agctcacgcg ccagaaccac gtagcgggat tcgcctaata
      541 ccgcaacgat atcttccagg ctatctaaca gacggtgggt agattcataa aaaatcagcg
      601 tgcgcggctc cgcttcaatg gcttttagcg catcacggcg gccttttgat ttggcaggta
      661 aaaagccttc gtaacagaaa cggtcagatg gtaaacccgc ggcgcttaac gcagtgatag
      721 cagcacacgg tccgggtagc ggcaccacac gaatccccgc ttcacggcag gtacgcacca
      781 gatggtagcc aggatcgtta attagcggcg ttccggcatc ggaaaccagc gcaatgtttt
      841 gcccctcttg cagcttcgcc agcagcgttt cggctttttg ttgttcgtta tggtcgtgca
      901 gcgcaaacaa ccgggcatta atcccaaaat gttgcagcaa taaaccggtg tgacgagtat
      961 cctcggcggc aatcagatca acggcctgta atacctctaa cgtacgctgg gtgatatccg
     1021 ccagattgcc gattggcgtc ggtacaatgt aaagctggcc ctgagaatta tccgccgatt
     1081 ggtgttgttt cattgtgtcg tccgtattgc cgatttaata ttgagcattg cgtaaaaaaa
     1141 atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc gcgcgttgtc
     1201 tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact cccgatcagt
     1261 ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt cagcagatgc
     1321 agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt gcactggtga
     1381 aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa gaactgaacg
     1441 attctcagcg tcgcgagaaa acactgctgg cggcagagat taaactggcg cagaaagatt
     1501 ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa caaaaccagc
     1561 aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc attgatttac
     1621 tgcgcgcgtt aattgctcag gaaccgctgc ttggcgcgaa agaaaaacag cagaatattg
     1681 atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg ctggtgatca
     1741 acgccgacga aaatattctg caaggctggc tggctctgca gcgcgtctgg tttgataacc
     1801 gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcagaaacgt tatccgaaca
     1861 atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt aaaccagcct
     1921 cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta tttggtcgca
     1981 ctattcagca aggctttgaa gccgcgaaaa atatcggcac tcagccagtg gcggctcagg
     2041 tagctgccgc acctgccgca gacgtagcag aacaacctca gccgcaaact gcggatggcg
     2101 ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct gcggcccagc
     2161 cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc gcaaatccat
     2221 ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc ttaagccagg
     2281 ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac gttgaagagt
     2341 tgctgaagag caacaccccg ctgagcgtac tggcactgaa ccagccggag aatatcgaaa
     2401 accgcgtcaa tatttgttac ttcgcgcttt caccggaaga cgaagcgcgc gatgcagcgc
     2461 gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc agtgcattgg
     2521 gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc ggcaccgttc
     2581 tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc ggttctggta
     2641 ttgctttaac gggtagcccg attactccca gagcgacaac cgactccggc atgacgacca
     2701 acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat ggcggtcgtg
     2761 tcgatgcggt ctacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa ccgatgatcg
     2821 ccatgcgtaa cggtagccag agcggtgcaa cgctgtacgc cagttcccgc agcgcacaag
     2881 gcaccgcagg cccggatttc cgactggaga tggaaggctt gcagtacagc gaaatcccga
     2941 tgctggcagg cggtaatctg ccgttaatgc agcaggcgct cagcgcggtg aacaacgatt
     3001 attcgctggc tcgcatgtat gcgatgggcg ttgatgcctg gtcgttggca aatcatttct
     3061 cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagt ctgacggcta
     3121 acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa ggtcaggtag
     3181 tccccgccag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag cgcgtcgctg
     3241 gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg gcggcgagat
     3301 cgatctgata atgcgtgaag gcctgaccac cgtttttgtc gaggtacgct atcgccgctc
     3361 tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca aattattaca
     3421 gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg attgccggtt
     3481 cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct ttaatgacca
     3541 ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc ttcactgaaa
     3601 gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc cgtgcagcca
     3661 tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt aatggaactt
     3721 ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa acggagcggc
     3781 ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg attgccaacg
     3841 atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat gcgggagatg
     3901 tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca gttgaagccg
     3961 ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc gaacttgcag
     4021 gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt gctcgcattc
     4081 aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac acgcttttcc
     4141 ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca gtccttattt
     4201 ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct gctgtgggta
     4261 ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt accctggaag
     4321 tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc cgtattaatg
     4381 tcacggccta tcagggcaaa gtgctgctgg ttgggcagtc accaaatgct gaactttcgg
     4441 ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat aacgagattc
     4501 gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc accaccaaag
     4561 tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa gtgaccaccg
     4621 aaaacggtga agtgttcctg atggggctgg taactgaacg tgaagcgaaa gcggcggcag
     4681 atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg tttattaaat
     4741 agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc tacaacgatg
     4801 caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc tcaaatcaga
     4861 acaacagcgc cagcccgccg acaatcacac cagacactgc caccatcgcc cctgtcagcc
     4921 ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa ctcactgccg
     4981 ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc atcatcgttt
     5041 gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct accgccatcg
     5101 ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat aaccagacgc
     5161 gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc cagaaaagtg
     5221 tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt atgtcaattt
     5281 cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc gtcgcaatca
     5341 gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag ccgaggacaa
     5401 accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag aatgccagcg
     5461 caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc gcacagcagg
     5521 tacacatcat gcccggcagc gaaaacagcg ttcccggcag cgtgccgcga aagcgcgatt
     5581 gcccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac gaaccgagga
     5641 tcacccccag caccgccgct ttccatactg cgaggaaata gatcatcgcg taatccaacg
     5701 ccgcttgcca cggattagca tccgcctgcg caaggataga tttaccgata ctgtgggttt
     5761 cggctgcagt aaacgctttg ccgtagtaag gttgccattt cacataccag agaccggcaa
     5821 tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc gcctgagatg
     5881 aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc gcgtgaatat
     5941 tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat tctgaagagc
     6001 ttaaaatcgt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg acccgcgcca
     6061 cgtctctcgc atcaatggat ttccagttac ctggtaacaa acggaacagc ggcgcaaaaa
     6121 gcgtttcgtt catccgctgt ttgctacgat cgcccagtaa catcgacggg cgagcaatgg
     6181 tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg acgcggttat
     6241 agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata tgctgcgcgc
     6301 ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca tgaataaacg
     6361 cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaataca atgtcgatgg
     6421 gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta aacaccccgg
     6481 gcatatcgcc caacggacgt cgcgtcggcg cagcaatggc gttaactttc ggttcattaa
     6541 tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc agtacctgac
     6601 tcatcttcgc tcctttacag aattgtctgc cttgcgctcc ccggctcatg caccagtctt
     6661 aatagaccat gaggtaatta tccccgattg tggaaaattc gcctcatcca atgcaacaac
     6721 gcggaggaag catgagtaag aaaattgccg ttttaatcac tgatgaattt gaagattcag
     6781 aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt actattgaaa
     6841 aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc atcgataaat
     6901 ccatcgatga agtgacgcca gcggagtttg atgccctgtt gctaccgggt ggtcattcac
     6961 cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt gtgaatagcg
     7021 gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc gatgtgattc
     7081 gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat gcgggcgtgg
     7141 aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc cggacaccgg
     7201 acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga gtcgcgtagc
     7261 caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc atcgagccgg
     7321 atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt gtacgccttg
     7381 cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa ctgcacaccg
     7441 cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc agcctgcggc
     7501 ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc gactttctgc
     7561 gcatcaaaaa gataaaaggc attagcgcac cagagttccc cttcctgctg cacacaccag
     7621 gtgacaacat gttgttttgc cagccaacgg ctgatggcga tgagtgtttc cattgctgtt
     7681 ctcttttata ctgtgggcct gaccttaaca tgcgatgcag acgatgaccc cctggtttct
     7741 ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg atgtcgaacg
     7801 ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga aaggagaact
     7861 gacgctggcg ttttctgcgc cagtgggcga ccgttcgctg gcactacggg cggaatatcg
     7921 cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg cagggtttgc
     7981 agaactgttg agcagcctgc aaaccccgga gattaaaagc gattgaaatg ctcgtgatac
     8041 tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac ctggaaggcg
     8101 ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc aaagccgaaa
     8161 cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa ctcattaagc
     8221 gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta tttttcatca
     8281 accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc aaccggacta
     8341 aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc cagcgtcaga
     8401 aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc gaatgagcga
     8461 cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat tagcatggtt
     8521 cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca acaaaatccg
     8581 ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca atggcgtcca
     8641 tcaggttttt cacatacacc cccagctccg tatcgccttc aatcaccagc cgacgctgga
     8701 agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg ctggcgtcgg
     8761 cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca ttcaccaccg
     8821 aggtaaacca ttgcaggtca atatcacaca catgaatact taaccagcgg ccttcaagaa
     8881 actccagctc gccatcatcc agcgcctgac ggaattgcca gcttaagacc tgctcaagaa
     8941 cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga gatggcccca
     9001 aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt ttcaacaatc
     9061 atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat tccatatgaa
     9121 attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc gcagcaaggt
     9181 taactaaaat cccagttcgt taacattttt gcgttttgat agcgcaacct tcaggaaaaa
     9241 ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc atcgaaaacg
     9301 gcgcagatgc tgtttatatc gggctaaaag atgataccaa tgcccgtcac ttcgccggcc
     9361 ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa catcgccgca
     9421 aacttcacat ctcgattaac acttttgcgc atccggacgg ttacgcccgt tggcagcgtg
     9481 ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac ctcgccatgc
     9541 tggagtacgc cgccgagcgt tacccgcata ttgaacgtca tgtgtcagtg caggcttcgg
     9601 cgaccaatga agaggcgatt aacttttatc atcgccattt tgacgttgcc cgcgtggtgc
     9661 tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggttact cctgtcccgc
     9721 tggaagtgtt tgctttcggc agcctgtgca ttatgtcgga aggccgttgc tatctgtcgt
     9781 cgtatctgac gggtgagtcg cccaacactg tgggtgcgtg ttctccggcc cgtttcgtgc
     9841 gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg atcgaccgtt
     9901 atcaggacgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat ctggtggacg
     9961 gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa ctgctgccgg
    10021 agttaatggc tgcgaatatt gcttcggtga aaattgaagg ccgccagcgt agcccggcgt
    10081 atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag gccgatccgc
    10141 aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc gaaggcacgc
    10201 aaaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga aatattcctt
    10261 agggccagtg ctgtggtact ggccaaaaga gacgctggaa gatttttatc agcaagccgc
    10321 cgccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc gccgggcaac
    10381 caaagttggc gactgtctgg agatggcaaa atcgctcgcc ggaagtggca agcagattgt
    10441 gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga aacgctatgt
    10501 tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata tgtgcgctga
    10561 acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg tgacgctgaa
    10621 aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt cccgcgactg
    10681 gctggtgaat ctgcttaatc agtgcgatga gctgggcatt cgcaaccagt ttgaagtgga
    10741 agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta ccgcgcgttc
    10801 ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga acgggcgcaa
    10861 cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga ccatgagcgg
    10921 ctacgtttac aacctcggta acgagctggc atccatgcag ggattggttg atgtagtccg
    10981 cctgtcaccg cagggcactg acactttcgc gatgctcgac gccttccgcg ctaatgaaaa
    11041 tggcgctgtg ccactgccgt tgacggcaaa cagcgactgt aacggctact ggcggcggct
    11101 ggcaggactg gagctgcaag cctaagtaaa tagctcactt tgttaacaac tttaactatt
    11161 ctttaatgca gtattaaaga ttaatcggta acaaagtgag ctgttatgac tgataaaacc
    11221 attgcgtttt cgctactcga tctggccccc atacccgaag gctcttcagc gcgggaagcg
    11281 ttctcccact ctctcgatct cgcccgtctg gctgaaaagc gcggctatca tcgctactgg
    11341 ctggcagaac accacaatat gaccggcatt gccagtgctg caacatcggt attgattggc
    11401 tatctggcgg cgaataccac cacgctgcat ctggggtctg gcggcgtgat gttgcctaac
    11461 cactcaccgt tggtcattgc cgaacagttc ggcacactca atacgctcta tccggggcga
    11521 atcgatttgg ggctgggtcg tgctccgggt agtgaccaac ggacaatgat ggcgctacgt
    11581 cgtcatatga gcggcgatat tgataatttc ccccgcgatg tcgcggagct ggtggactgg
    11641 tttgacgccc gcgatcccaa tccgcatgtg cgcccggtac caggctatgg cgagaaaatc
    11701 cccgtgtggt tgttaggctc cagcctttac agtgcacaac tggcggcgca gcttggtctg
    11761 ccatttgcgt ttgcctcaca cttcgcgccg gatatgttgt tccaggcgct gcatctttat
    11821 cgcagtaact tcaaaccgtc ggctcggctg gaaaaaccat acgcgatggt gtgcatcaat
    11881 attatcgccg ccgacagcaa ccgcgacgct gaatttctgt ttacctcaat gcagcaagcc
    11941 tttgtgaagc tgcgccgtgg cgaaaccggg caactgccgc cgccgattca aaatatggat
    12001 cagttctggt caccgtctga gcagtatgac gtgcagcagg cgctgagtat gtcgttggta
    12061 ggcgataaag cgaaagtgcg tcatggtttg cagtcgatcc tgcgcgaaac cgacgctgat
    12121 gagattatgg ttaacgggca gattttcgac caccaggcgc gcctgcattc gtttgagctg
    12181 gcgatggatg ttaaggaaga gttgttggga tagtgtgtct taacgcggga agccttatcc
    12241 gagctggcaa cgctgtccta cgtaggcctg ataagcgaag cgcatcaggc attgtgtagg
    12301 cagcagaaat gtcggataag gcaccgctga ttactgatac accggcagta aattaaagct
    12361 cgataaaata tgcaccagtg cgttgccgac gccaaacacc agaatcagcg caatcatcgg
    12421 cttgccaccc cagacgcgga atttcgggct gccaaagcgt ttacgcgatg cgcgggccag
    12481 cagtgccgga acaattgccg cccagatggt agccgctaaa ccagcataac caatggcata
    12541 caggaatccg ttcgggaaca gcagaccacc cacaacaggc ggggcaaagg tcagcaatgc
    12601 cgttttcaag cggcccacag ccgagtcgtc gaaaccaaac agatctgcca gatagtcaaa
    12661 caaacccagc gttacgccaa ggaacgaact cgctaccgca aagtttgaga acacgaccag
    12721 cagaaggtcc aggctacggc tgttcagcac gccgcttaat gcttgtacca gcacatcaat
    12781 attaccgccc ttctcggcga tgccgataaa ctccggacga gggatgttgc ccattgtcgc
    12841 cagcaaccag atggtataca gcgccagtgc catcagcgta ccgtacacca ggcatttcac
    12901 gatggttttc ggatctttgc cgtaatactt catcaggctt ggcacgttac cgtgataacc
    12961 aaacgacgcc aagcagaacg gcagggtcat cagcaaatac ggcgcataag atgcattgct
    13021 ttcggcaacg ttgaacaatg tcgcaggctg cacatgcccc agcaggctac cgaaggtgag
    13081 gaagaaggta atgactttcg cccccagcac aatcgccgtc atgcggctga cggctttagt
    13141 actcaaccac accacaaacg ctaccagcag tgcaaaacca aaacccgccg cccgtgccgg
    13201 gacgtttagc gacatctctg cgaaggtgtg atgcagaatc gaaccactgg cagaaatata
    13261 ggcataggtc aggatataga gcacaaaggc aatggaaatg ccgttgacca cgttccagcc
    13321 tttgcccagc aaatctttgg tgatggtgtc aaaactcgaa ccgattcggt aattcaggtt
    13381 agcttccaga atcatcaagc cggaatgcag catacagaac caggtaaaga tcagcgctgc
    13441 cattgaccag aaaaaccacg ccccggacat gaccactggc agagaaaaca tccctgcgcc
    13501 aataatggtg ccgccgataa tcaccacgcc gccaagcagc gacggtgacg tttgggtggt
    13561 ggttagtgtt gccatgaggg cttctctcca gtgaaaaata gtgcgactgc gttgttatgc
    13621 attgcactgt accagtacat gagtacaaaa gacagaaaaa aagccccgat ggttaaaatc
    13681 ggggctgtat atattatttt acagattgtg ttcgctgttc agcgatgatt acgcatcacc
    13741 accgaaacga cgacgaccgg tagagtcatc acgacgcggt gcgcggcctt cacgacgttc
    13801 gccgctaaaa cgacgaccat caccacgacc accttcacgg cgttcaccgc tgaagttacg
    13861 accgccttca cgacgctcgc caccgaaacc acgaccaccg ccacgaagct caccgccagt
    13921 atgcggctgt gcatcgccca gtaactgcat gttcatcggc ttgttgagaa tgcgagtgcg
    13981 cgtaaagtgt tgcagcactt cacccggcat acctttcggc agttcgatgg tggagtgaga
    14041 agcaaacagc ttgatgttac cgatgtaacg gctgctgatg tcgccttcgt tagcaatcgc
    14101 gccaacgata tgacgaactt caacaccatc atcgcggccc acttcaatgc ggtacagctg
    14161 catatcgcca acatcacgac gttcacgacg cggacgatct tcacggtcgc cacgcgggcc
    14221 acggtcgtta cgatcgcgcg gaccacggtc atcacggtca cggaattcac gtttcggacg
    14281 catcggcgca tctggcggaa caatcagagt acgttcaccc tgtgccattt tcagcagtgc
    14341 cgcagccaga gtttcgagat ccagctcttc accttctgca gtcggctgaa ttttgctcag
    14401 cagtgcgcgg tattgatcca gatcgctgct ttccagctgc tgctgtactt tagcggcgaa
    14461 tttttccaga cggcgtttgc ccagcagttc tgcgttcggc agttctactt ccggaatagt
    14521 cagcttcata gtacgttcaa tgttgcgcag cagacgacgc tcgcggttct caacgaacag
    14581 cagcgcgcgg ccagcacgac ccgcacgacc ggtacgaccg atacggtgaa cgtaagactc
    14641 agaatccatc gggatatcgt agttaaccac caggctgata cgctcaacgt ccaggccacg
    14701 ggccgcaacg tcggtcgcaa tcaggatgtc cagacgaccg tctttaaggc gttccagtgt
    14761 ctgctcacgc agcgcctggt tcatgtcacc gttcagcgcg gcgctattgt agccgttacg
    14821 ctcaagagct tcagccactt ccagagtcgc gtttttggta cgaacgaaga taatcgccgc
    14881 atcaaaatct tccgcttcca ggaaacgtac cagtgcttcg tttttacgca taccccacac
    14941 agtccagtag ctctggctga tgtcaggacg ggtagtcacg ctggactgaa tacgcacttc
    15001 ctgcggctct ttcataaagc ggcgggtaat gcgacgaatc gcttccggca tggttgcaga
    15061 gaacagagcg gtctgatgac cttccgggat ctgcgccata atggtttcaa cgtcttcgat
    15121 gaagcccatg cgcagcattt cgtcagcttc atccagaacc agaccgctca gtttagagag
    15181 gtccagagtg ccacgtttca ggtggtccag cagacggccc ggagtaccga caacgatctg
    15241 cggcccctga cgcagggcgc gtaattgcac gtcataacgc tggccgccgt acagggcaac
    15301 cacatttacg ccgcgcatgt gtttagagaa atccgtcatt gcttcagcaa cctgtaccgc
    15361 cagttcgcgg gtcggtgcca gcaccagaat ctgtggtgct ttcagctcag gatcaagatt
    15421 ctgcagcaga ggtaaagaga atgctgcagt ttttccgctc cccgtctggg ccatacccag
    15481 aacgtcgcgg ccattcagca gatgtggaat acactctgcc tgaattggag atggtttttc
    15541 gtaacccaga tcgttaaggg cttcaaggat aggagccttc aggcccagat ctgcaaaagt
    15601 ggtttcgaat tcagccatgt agtacgtgtg cctcaaaatt aatggcggcc agtctacata
    15661 actcatcatg aaattgatca gcaattttca ttgaaaagtg tgaaccggct caaagtaggt
    15721 gtattaacga acaacaacgc cctcacccgt taaggtgatg gcaatcaaaa aagattacgg
    15781 gctgatgtgt acgtcagcta ttgctggtcc gattctgcca ggtcatcttg gtcctggccc
    15841 aggagcgata attccaacaa tgcgtatcgg tgctcaacaa agttatgaac gttgttggca
    15901 accgccagtt tgaacagtgc cgtggcgctg tccaaatccc ccagacttag gtagtactta
    15961 cctaaataga agttggtttc actgagatgc tcagcgagcg aggtgttatc ggttgcgtcc
    16021 gccttgagcc tttccattaa cgtgtgttcg ctaatgttgc ccaggtagaa ctcgacaatg
    16081 ttccatcccc actgttcctt atccgatttt tcgaagtgct gtttcaacac ttctttagcc
    16141 tgcttctcat cgagcttctg ctcggcgaga taaagccaca gactacggaa aggatcattg
    16201 ggatcgtctt gataaaacgc cagcagatca tcttgcgcta acttgtcacg accgccgtaa
    16261 tataatgcaa tcccgcgatt caagtgcgcg tagttgtaag ttggatcaag ctcaagtaca
    16321 gaatcaaacg cttcataggc agcatcaaaa ttgcctgcct gcgttaaata tatgcctaag
    16381 taattgaata cttcaggcat atccggtcgg attgccagcg cttgcgaaaa atcgttacgc
    16441 gctaatgccc tcagaccgag actatcatac aacactccgc gctcatataa aagctgtgcg
    16501 cgttcgtcat cggttaaagc ccgactggca aggatttgtt ccatacgtgc cagaatcact
    16561 tcctgctgta aagtcggttg caatggtacc gcgaggactt cacttttacg ccaggaagta
    16621 ttactgcatc ctgcaagcgt aagtgctgtc gcaacgaaac accagcgcaa aaaaggcttc
    16681 atttcccact cccgaagacc acggttgaat gaacgtcctg ttcccggttg ctaacaaggc
    16741 gtcctgcccg gttaaaagcc ccccgccgca gcggagggca aatggcaacc ttactcgccc
    16801 tgttcagcag ccggagcttc cggtgctgca gcaggttgag actgctcagt cgcttcttta
    16861 atgctcagac ggatacggcc ctggcgatca acttccagaa ctttcaccgg tacttcctga
    16921 cccatctgca ggtaatcggt cactttctca acgcgtttgt cagcgatttg agagatgtgt
    16981 accagacctt ctttaccgcc gccgatggca acaaatgcgc caaagtcaac gatacgggtc
    17041 actttaccag tgtagacgcg gcccacttcg atttctgcag tgatctcttc gatacgacga
    17101 atagcatgtt tcgctttctc gccgtcggtc gctgcaatct tcacagtacc gtcatcttcg
    17161 atttcgatgg tggtgccagt ttcttcggtc agggcacgga taacagagcc gcctttaccg
    17221 ataacgtctt tgatcttgtc cgggttgatc ttgatggtat ggatacgcgg tgcgaactca
    17281 gagatatcgc cacgcggcgc gttgatcgcc tgttccatta cgcccaggat atgcagacgc
    17341 gcacctttag cctggttcag cgcaacctgc atgatctctt tggtgatacc ttcaattttg
    17401 atatccatct gcaatgcaga gataccctcg cgggaacccg caactttgaa gtccatatcg
    17461 cccaggtgat cttcgtcgcc caaaatgtca gacagtacaa cgtagttgtc gccttctttc
    17521 accagaccca ttgcgatacc cgcaacggca gctttgatcg gcacacctgc gtccatcagc
    17581 gccagagacg cgccgcacac ggaagccata gaagaggaac cgttggattc agtgatttca
    17641 gacacaacac gtacggtgta cgggaatttg tccatatccg gcatgactgc cagcacgccg
    17701 cgcttcgcca gacgaccgtg accaatttca cgacgcttcg gagaaccgac catgccggtt
    17761 tcgcctacgg agtacggagg gaagttgtag tggaacagga aggtatcggt acgttcgccc
    17821 atcagttcat caagaacctg cgcgtcacga gcagtaccca gcgttgcggt aaccagcgcc
    17881 tgcgtttcac cacgggtgaa cagcgcagaa ccgtgagtac gcggcagcac gccagtacgc
    17941 acatccagac cacggatcat atctttttca cgaccgtcga tacgcggttc gcctgccagt
    18001 acgcggctac gaacaacgtt tttctcgatc gcgtgcagaa tttcacccag ttcgttttcg
    18061 tccagggttt cgtcttcagc aagcagcgtc gcgatggttt cagatttgat gacatcaacc
    18121 tgcgcataac gctcttgttt gtcggtgatg cggtaagcat cgctcaggcg agcttcagcc
    18181 agtgcagcaa cgcgcgcgtt cagcgcttcg tttaccggct ccggctgcca gtcccaacgt
    18241 ggtttaccgg cttctttcac cagttcattg atgttctgaa taacaacctg ctgttgttca
    18301 tgaccgaaca ctactgcgcc cagcatctgg tcttcgctca gcagttcagc ttcagattca
    18361 accatcagta cagcggcttc agtaccggca acaaccagat ccagtttgct ctctttcaac
    18421 tcgtcctgag tcgggttcag tacgtactgg tcattgatgt aacctacgcg agcagcacca
    18481 atcggaccat tgaacggaat accagacaga gacagtgctg cggaagcacc aatcatcgcg
    18541 acgatatccg ggttaacttg cgggttaaca gaaaccacgg tggcgataac ctgaacttcg
    18601 ttgacgaagc cttccgggaa cagcgggcga atcgggcggt caatcagacg cgcgatcagg
    18661 gtttcgcctt cgcttgggcg gccttcacga cggaagaagc tacccgggat acgaccagca
    18721 gcgtaggtac gctcctgata gttaacggtc agtgggaaga agtcctgacc tggtttggct
    18781 tttttctggc caacaacggt aacaaatacc gcggtgtcat ccatgctaac cataacagcg
    18841 gcagtagcct gacgagccat catgccggtt tccagagtca cggtgtgttg gccgtactgg
    18901 aatttacgaa cgatcggatt aagcaatgta atatcctttc tctttcttag acagtacctt
    18961 acggcactgg tgttaatacc cgatcttctg cgcatcctcg cgactaatga caaccctaac
    19021 ccagctctat gtgggtaaag cctctcatta gccgcgcgaa cctctgcaac ggaagatcat
    19081 tcatagcaac aatacattag tttccagtga attgctgccg tcagcttgaa aaaaggggcc
    19141 actcaggccc ctttttctga aactcgcaag aattagcgac gcagacccag gcgctcgatg
    19201 agctgggtgt aacgtgctac gtctttacgt ttcaggtagt cgagcagttt acgacgctga
    19261 gaaaccatgc gcagcagacc acgacggctg tggtgatctt ttttgtgctc tgcaaagtgg
    19321 ccctgcaggt ggttgatctg tgcagtcagc agtgctacct gaacttcagt agaaccggtg
    19381 tcgtttgcgt cacgaccaaa ctcagaaacg attttagctg ttgcttcagt acttagagac
    19441 attttaaaac tccaaagtat atagaatgaa aggacgccga tctctaattc agcgatccca
    19501 gtgtacgtta cgcaaagtgt taaacaattt acgcgacgtt aagcggcagt attctactcg
    19561 tagcgacctg ttatcgcaag acggttaaca ttacgccggg tattcaacca ccaggcgacg
    19621 aggcgcaacg cggccttcat cgtcaatttc acccataccg ataaatttgc cgttctcacc
    19681 ttctgtaacg cgaaccagtc cttccagcgg cgcaccggat gtacgaactg ggttaccatt
    19741 tttgaagtaa acagaagacg ttaacggaag attcaccacc gggtagtccg aagctggact
    19801 gtccattggc atcagtaatg gatcaagtaa ctccgcagcc ggaatatcct gctgttcggc
    19861 ttgttcgacc agttcacgca gatgttccag ggtcacaatc cgttcaaccg gatatttact
    19921 taccgccaga cgacgcaggt aaataacatg cgcgccacaa ccgagttttt cacccaggtc
    19981 atcaatgatg gtgcggatat aagtgccttt tgagcagtga atttccagct ccagctcatt
    20041 gccttcgtgg cgaataaaca gcaattcata aacggtaatc gggcgcgctt cacgcggtac
    20101 ttcaatgccc tgacgcgcat attcgtacag ttttttaccc tgatatttca gtgctgaata
    20161 catcgaaggg atctgttcga tatcgccacg gaaagtatcc agcgccgccg ccagttgctc
    20221 tgcgctaaag gttaccggac gttcttcaac gatttgtccg tcggcatcag aagtatcggt
    20281 acgctgtcca agacgcgcaa tgacccgata gcgtttgtcg gagtccagca gatactggga
    20341 aaacttcgtc gcttccccga ggcaaattgg caacatgcca gttgccagcg ggtccagcgc
    20401 accggtatgc ccggcacggt tggcgttata aatccgtttc actttttgca gcgcatcatt
    20461 gctggacata ccctgcggtt tatccagcaa caaaacgccg ttaatgtcgc gaccgcgacg
    20521 acgaggacga ctcattagtc ctccttgctg tcgtccgggt taacacgacg ttcttcgtca
    20581 tgtttgacca cgctggtcac caggtttgac atgcgcatcc cttcaaccag agagttgtcg
    20641 tagaagaagg tcagttccgg cacgatacgc aggcgcatcg ctttccccag caggctgcgg
    20701 atgaaaccag aagcttcttg caacgctttg atgcccgctt taaccgcgtc ttcatctttg
    20761 tcgttgagga acgtcacata tactttggca tacgccaggt cgcgagacat ttcgacaccg
    20821 gaaacggtgg tcatcatgcc caggcgagga tctttaattt cacgctgcag gatgagagcg
    20881 atctcttttt gcatttcctg cgctacgcgc tgcgggcgac caaattcttt cgccataata
    20941 aattctcctg acaaaaaagg ggctgttagc ccctttttaa aattaatttc aggtggaagg
    21001 gctgttcacg ttggcctgat aagacgcgcc agcgtcacat caggcaatcc atgccggatg
    21061 cggcgtaaac gccttatccc gcatggaacc ctaaaaacct taagcgatgg tacgttggat
    21121 ctcgatgatt tcgaatactt cgatcacatc gccagtgcgg acgtcgttgt agttcttaac
    21181 gccgatacca cattccatac cgttacggac ttcgttaacg tcatctttga agcggcgcag
    21241 ggactccagc tcgccttcgt agataaccac gttgtcacgc agaacgcgga tcgggttgtg
    21301 acgtttaacc acaccttcgg taaccataca gcctgcgatg gcaccaaatt tcggcgattt
    21361 gaacacgtca cgaacttccg ccagaccgat aatctgctgt ttcagttccg gagacagcat
    21421 accgctcatc gccgctttca cttcgtcaat caggttatag atgacggagt agtaacgcag
    21481 atccaggctt tccgcttcaa tcactttacg tgcagaggca tcagcacgta cgttgaagcc
    21541 aaccaggatg gcgttggacg ccgcagccag ggtggcgtcg gtttcggtga taccacctac
    21601 gccagaaccg atgatcttca ctttaacttc gtcagtagac agtttcagca aggagtcgga
    21661 gatcgcttcc acagaaccct gtacgtctgc cttcaggacg atgttcactt cgtgaacttc
    21721 gccttcggtc atgttggcga acatgttctc gagtttagat ttctgctgac gcgccagttt
    21781 aacttcgcgg aatttaccct gacgatacag tgcaacttca cgcgctttct tctcgtcacg
    21841 tacaacggta acttcatcac ccgcagccgg tacgccggac aggccgagga tttccaccgg
    21901 aatggacgga cccgcttcca gcacttcctg acccagttcg ttacgcatcg cacgaacacg
    21961 accgtattcg aagccacaca gaacgatatc gcccttgtgc agagtacctt cacgtaccag
    22021 aacggtagca accggaccac gacctttatc gaggaaggat tcgataaccg caccgctcgc
    22081 cataccttta cgtaccgctt tcagctccag aacttccgcc tgcagcagga tagcgtccag
    22141 cagttcatcg ataccagtac ccgctttcgc agacacatgt acgaactggc tttcaccgcc
    22201 ccactcttcc ggcaggatgc cgtactggga gagttcgttc ttaacgcgat ccggatcggc
    22261 ttctggttta tcgatcttgt tcactgcaac gaccaccgga acctgcgccg ctttcgcgtg
    22321 ctggattgct tcgatcgtct gcggcattac gccgtcgtcg gcagcaacaa ccaggactac
    22381 gatgtccgtt gcctgcgcac cacgagcacg cattgaagta aacgcggcgt gccccggggt
    22441 gtccaggaag gtgatcatgc cgttttcagt ttcaacgtgg tatgcaccaa tgtgctgagt
    22501 aatgccgccc gcttcgccag aggccacttt cgttgaacga atgtagtcca gcagagaggt
    22561 tttaccgtgg tcaacgtgac ccatgatggt cacaaccggc gcgcgcggtt cagccgcagc
    22621 acccgtgtca cggtcgctca ttaccgcctc ttccagctcg ttttcacgac gcaggataac
    22681 tttatggccc atctcttcag caaccagctg tgcggtttcc tgatcgataa cctggttgat
    22741 ggttgccatt gcgcccagtt tcatcatcgc tttgatgacc tgagagcctt taaccgccat
    22801 cttgttcgcc agttcgccaa cggtgatagt ttcgccgatc acaacgtcac ggttaacggc
    22861 ctgagcaggc ttctggaagc cttgctgcag cgaagaacct ttacgttttc cgcctttacc
    22921 gccacgtact gctgcgcgtg cttcttcacg atcagctttt gattcagcgt gtttgttgcc
    22981 tttcttcgga cgcgctgctt tcgcgttacg accacggcca cggccgcctt cgacttcacg
    23041 atcgctttcg tcttctgcct ggcgagcatg ttgagaagta gtgacgtgat aatcgctgga
    23101 atcttcagtc ggttccgcgt tatcagtcca tttgttttct tccgccatac gacgtgcttc
    23161 ttcagcaacg cgacgtgctt cttcttcgag tttacgacgc gcttcttctt cagctttacg
    23221 cttgagctct gcagcttcct gctcacggcg ggctttttca gcctgggcgt ttttagtcat
    23281 atcgtcttgt tgattgctca ctttgtcttt ttccgcagct tcacgtttcg cttgttcagc
    23341 agcttcacgc ttagcttgtt ctgcggcctc acgttcagct ttttgttgcg cctcgcgttt
    23401 agccgattct tctgcctcac gacgggcttg ctcttccgct gcaaggcgtt cagcctcttg
    23461 cggatcgcgt ttcacaaagg tgcgtttctt gcggacttcg atttgtaccg atttgctttt
    23521 tccaccggta ccaggaatgt taagggtgct gcgtgtttta cgttgcagcg tcaatttgtc
    23581 cgggcctgaa tttttctgat tcaggtggtc aatcaaagtc tgtttctctt gtgcagacac
    23641 agagtcgtca gcagacttcc ggatacctgc atcagcaaat tgctgtacca ggcgttccac
    23701 ggaggtctgt cgctctgcgg ccagcgtttt aatcgttaca tctgtcatgc tgttccttcc
    23761 tgctacagtt tattacgctt cgtcaccgaa ccagcaaata ttacgggcag ccataatcag
    23821 tgctccggct ttttcgtcgg tcaacccttc gatatcagcc agatcatcaa tgccctgttc
    23881 ggcgagatct tccagcgtac aaacgccacg ggcggccagt ttgaatgcca aatcacgatc
    23941 taccccttca aggttcagca gatcgtcagc cggtttgtta tcaccgaggc tttcttcctg
    24001 ggcctgtgca atggtggcca gtgcattttt agcacgctcg cgcagtgctt caacggtcgg
    24061 ctcatcaagg ccttcgattt ccaacagctc tttcatcggc acataggcca gttcttccag
    24121 cgtcgagaaa ccttcttcta ccagaacagt cgcgaagtct tcgtcgatgt cgagatattt
    24181 ggtgaaggtg tcgatcgctg cgtgcgcttc cgcctgatgc ttagcctgca ggtcgtcaac
    24241 ggtcattacg ttgagttccc aaccgctcag ctgcgaagcc agacgcacgt tctgaccgtt
    24301 acggccaatc gcctgcgcca ggttaccggc ttcaacggcg atatccatgg tgtgtttatc
    24361 ttcatccacc acgatagaag caacgtctgc cggtgccatt gcgttaatca caaactgcgc
    24421 cgggttatca tcccacagga cgatatcgat acgctcgccg cccagttcag tagaaaccgc
    24481 ctgaacacgc gcgccacgca tacctacgca agcacctacc ggatcgatac gtttatcgtt
    24541 ggttttcacc gcgattttcg cacgagaacc cgggtcgcga gccgctgctt taatttcaat
    24601 cacttcttcg ccgatttccg gcacttcaat acggaacagt tcgatcagca tttccggctt
    24661 ggaacgagtg acgaacagtt gcgcgccacg cgcttccggg cgaacggaat aaagcacgcc
    24721 acgaacgcgg tcgccagggc ggaagttttc acgcggcagc atatcttcgc gcaggatcac
    24781 ggcttcagca ttgttgccca gatccagaga gatgttgtcg cggtttactt ttttcaccac
    24841 gccggtgatg atttcacctt cgtgttcacg gaactgatca accaccatcg cacgttcggc
    24901 ttcacgcact ttctgcacga taacctgttt tgccgtctgg gtggtgatac ggtcaaaggt
    24961 aacagactca atctgatctt caacgtaatc gcccaagttc aggctttcat cttcataacg
    25021 tgcggcttca agggtgattt ccttggtcgg ctgggtgact tcatcaacaa ctaaccagcg
    25081 acggaaggtg tcaaaatcac cgcttttgcg atcgatctgt acacggacgt cgatctcttg
    25141 ctcatatttt ttctttgttg ctgtcgccag cgcgctttcc aatgcttcga aaatcttctc
    25201 gcgaggtagc gccttttcat tggatacggc ttcaactaca gccaaaattt ctttgttcat
    25261 cgcgggcttt tcacctcatc cagactatta aaagtgggga accaggttcg ccttctggat
    25321 attactcagc gcgaacactt catctttacc ttcgacggta actgtgatca tttcaccgtc
    25381 taccgctttg ataacgccct gccatttacg acggttttgt accgccatac ggagaaccag
    25441 agtcacctct tctccgacaa aacgggcgta gtgttcagcc gtgaacagtg ggcgatcgag
    25501 acccggtgag gagacttcca ggttataagc aacggtgatg ggatcttcaa catccagcac
    25561 agcacttacc tggtggctca catcagcaca atcatcaaca ttgatgccat cttcactatc
    25621 aatatagatg cgcagtgtgg atgtgcgacc gcgaataaat tcgatgccta ccagttcaaa
    25681 acccagggcc tcaactggcg cagtaatcat ctctgttaat ttttgctcta atgtggacaa
    25741 gcccaccccc aagacataaa aaaagggcct aaagcccagt tattctgtag tcagataaca
    25801 aaaaaccccg ataaatcggg gctttatata actgaaccct ataaccgcaa ctgcggtctg
    25861 gagcactttc cagaaggatt ttttcaaatc ccactacgaa ggccgaagtc ttcacagtat
    25921 atttgaaaaa ggactctaag ggaaagtggt tgcgggggcc ggatttgaac cgacgacctt
    25981 cgggttatga gcccgacgag ctaccagact gctccacccc gcgcctgaaa cgtggcaaat
    26041 tctactcgtt ttgggtaaaa aatgcaaata ctgctgggat ttggtgtacc gagacgggac
    26101 gtaaaatctg caggcattat agtgatccat gccacatttt gtcaacgttt attgctaatc
    26161 acgtgaatga atatccagtt cactttcatt tgttgaatac ttttgccttc ccctgctctc
    26221 ccttaagcgc attattttac aaaaaacaca ctaaactctt cctgtctccg ataaaagatg
    26281 attaaatgaa aactcattta ttttgcataa aaattcagtg agagcggaaa tccaggctca
    26341 tcatcagtta attaagcagg gtgttatttt atgacgacga ttctcaagca tctcccggta
    26401 ggtcaacgta ttggtatcgc tttttccggc ggtctggaca ccagtgccgc actgctgtgg
    26461 atgcgacaaa agggagcggt tccttatgca tatactgcaa acctaggcca gccagacgaa
    26521 gaggattatg atgcgatccc tcgtcgtgcc atggaatacg gcgcggagaa cgcacgtctg
    26581 atcgactgcc gcaaacaact ggtggccgaa ggtattgccg ctattcagtg tggcgcattt
    26641 cataacacca ccggcggcct gacctatttc aacacgacgc cgctgggccg cgccgtgact
    26701 ggtaccatgc tggttgctgc gatgaaagaa gatggcgtga atatctgggg tgacggtagc
    26761 acctataaag gaaacgatat cgaacgtttc tatcgttatg gtctgctgac caatgctgaa
    26821 ctgcagattt acaaaccgtg gcttgatact gactttattg atgaactggg cggccgtcat
    26881 gagatgtctg aatttatgat tgcctgcggt ttcgactaca aaatgtctgt cgaaaaagcc
    26941 tactccacag actccaacat gcttggtgca acgcatgaag cgaaggatct ggaatacctc
    27001 aactccagcg tcaaaatcgt caacccgatt atgggcgtga aattctggga tgagagcgtg
    27061 aagatcccgg cagaagaagt cacagtacgc tttgagcaag gtcatccggt tgcgctgaac
    27121 ggtaaaacct ttagcgacga cgtagaaatg atgctggaag ctaaccgcat cggcggtcgt
    27181 cacggcctgg gcatgagcga ccagattgag aaccgtatca tcgaagcgaa aagccgtggt
    27241 atttacgaag ctccggggat ggcactgctg cacattgcgt atgaacgtct gttgaccggt
    27301 attcacaacg aagacaccat tgagcagtat cacgcgcatg gtcgtcagtt gggccgtctg
    27361 ctgtaccagg ggcgttggtt tgactcccag gcgctgatgc tgcgtgactc tctgcaacgc
    27421 tgggttgcca gccagatcac tggtgaagtt accctggagc tgcgccgtgg gaacgattat
    27481 tcaatcctga ataccgtctc agagaacctg acctacaagc cagagcgtct gacgatggaa
    27541 aaaggcgact cggtgttctc gccagatgat cgtattggtc aattgaccat gcgtaatctg
    27601 gatatcactg atacccgaga gaaacttttt ggttatgcca aaactggcct gctttcctcc
    27661 tctgccactt caggcctgcc gcaggtggag aatatggaaa acaaaggcca gtaattcgac
    27721 atcaaccctg cccttcaggg gtggggttac cttgcttatc ctctgtaaac tcctttctga
    27781 gcatcacata atcgaaaaca tcatggtttg catccacaac ccgtgagtct cctttatagt
    27841 gtgcaatcac ttcctcaagc gtttgcggct gttccggctt tgttaccccc atccacgcat
    27901 taataaggta attattgtaa gctgtcgaaa agatattgtt ttccgttgtg cgatccacgc
    27961 catccccaag aacggggcta taacagataa acatcgggac atgatatgcc tgctggctag
    28021 cctccctgcc tccatgaaaa tagacgttct tcttcgtagg gtcacgttcc aggccgtggt
    28081 cggcaaaata catgaccgag gcgcggcgat cttttaataa ttcaaaaaac tgacctagca
    28141 aactatctgt gtaatgaatg gagttgtcat agcaggcatc ctgatcgtcc tgcggttgaa
    28201 acacgcgctg gattgcggat aggcgctaca agccggttca tggcttccat ttaaatgaag
    28261 aacaatcagt ttcttttgct gcgtattttg ctgtaatgct tgcgataaat gcggcaacaa
    28321 caattcatca aatcctctga cataaactgt ttccatggcg cgcatggcga tactggtaac
    28381 tgctgtacca ttctgccgaa aagcggattg cgagcttagc cagaaagtct gaaatcctgc
    28441 ttgattagcc atattaataa tgttgtcggg gtaattatga atgtcatgac tcaaaacaga
    28501 atcagcagtt aaagaaaggg gaaccgacag cgcagtgtaa ggtgcgccgc ttattgcttg
    28561 attaaacagt ttgatctgtt ttctttgtgc ttcaacttgc ggtgtcgtag agcgtgtata
    28621 tccatacaaa gacatattgt cgacacgtac agactccccc acaatcaaca cgtaggtatc
    28681 aatacttgta tccctgactg ataattgaaa atacggcacc gtgtttgcaa ttgagagtaa
    28741 tctttgatgc tctttcgctg ctaaagcaaa atagttgaga ttgaaaaacg gcgtataggt
    28801 agcaaatcac gacgctagta tatatggact gaacgctttt ttattttttg catctttata
    28861 agcaaattga caagcggaaa ataaactgcc cgaaatgaca atcagcaata atattcctgt
    28921 cacttttttt gtcggcaagg aaacatcata ttttattatt actgccaaaa aaaaaaggga
    28981 taaaaaagca aaagcacata gataagggat atacatcccc agcattttca caacttcatc
    29041 cggatcactc tgcagcacac taatcgcaaa tccatcattg aaagttgtac caaaggtaca
    29101 ccatgaatag aggctaatac tcatgtctgc cgcagtcagt aacacaaatg gtgcggcaac
    29161 aagcaggcga agcggaaggg atgatatacg tttaacaacc aggatggtca ggacgaagaa
    29221 cagcaatcgc tgaaccatat gcgctccgga agaggcgact aagttcgtaa taccaaaaac
    29281 aataacacaa agatacaaca accaatgaga tttaaaatct tctagcaaat ttgttgaata
    29341 ctgtcatcca gaaatccaag ttcacaacca atataaacaa ttatcaacaa tgttcaattt
    29401 tatatcagac atgtttttca actctgaacc atatcagacc gaaacactgt tagttttttc
    29461 tttttaaatg atgcgaccat tgcgttctca gtaattaaat aacattatca atgcgtctct
    29521 aaacattacg cagaacgtag ccatcagtag ttatactgac aggagcagga tggtttatcg
    29581 agaagtaaac atatccgtat tcgtaccgga actggagttg acacaataaa gtgccaatta
    29641 tgtcagcagt agggaaaagt ttataactaa ggcgtaccgg caccatcgtt tcaaggtacc
    29701 agctacgagt aaagcaactg gacgagatac agatacctga caaccattca tcagaccagg
    29761 accaaaacga aaagagacgc ttttcagcgt ctcttttctg gaatttggta ccgaggacgg
    29821 gacttgaacc cgtaagccct attgggcact accacctcaa ggtagcgtgt ctaccaattc
    29881 caccacctcg gcacggatac tactttttag ttcgggatat cgctggtcgg cttagccgga
    29941 gcagctggct gagtttgttc ggttttcgcc ggtgcactca gattttccca ttcgctacct
    30001 ctattggttt tgttgctatt gatgttaccc agcaccagac tgatgatgaa gaataacgtt
    30061 gccagcagcg ccgtcatgcg ggtcatgaag ttaccagaac cacttgaacc aaacagcgta
    30121 gcggaagcgc ctgctccgaa ggaggctccc atatcagcgc ctttaccttg ctgcagcatg
    30181 atcagaccaa caaggccaat tgccacaata aggaaaacta ctaaaagagc ttcgtacata
    30241 atcaacctgt tccttgcgga gttgccgcgt accaatgctt caaccaataa agcgggagtt
    30301 tttaatcttt cccactgaag cgggtgtgaa tactaaccaa agcgaatgac cttcgcaagg
    30361 gcaattttat cgcattgtat caactgcgga aaaaaacagc aaaaccgatg tgttcgccga
    30421 gaaaaaaggc gacaatcgcc gcctttttag ccagttatct aacgctttaa acggctttta
    30481 ctgcatcggc gatgcggtgt gcaaattcag tcacctgcgc ttcgtcttcg ccttccacca
    30541 tcacgcgaat taacggttcg gtgccggatt tacgcagcaa cacgcgtcca cggttgccca
    30601 gcgcagcttc aacctctgcg gtcacggctt taactgactc atgctcaagc ggatcgccgc
    30661 tacctgcggt gtaacgtacg ttaaccagaa tctgcgggaa cattttcatg ccgctgcaaa
    30721 ggtcgtgcag gctcatatgg ttacgtgcca tcgccgccag cacctgcaag ccagcaacga
    30781 tgccgtcacc ggtagtggtt ttatccagca ggatcacatg accggaattc tctgcaccga
    30841 tacgccagcc tttctcctgc attttttcca gtacgtagcg gtcacccact ttcgcgcgcg
    30901 caaatggaat tcctaactgt ttcagcgcca gttcaagccc catgttgctc atcaatgtac
    30961 ccacagcgcc accacgcagc tggccctgac gaagaccttc acgcgcgatg atatacatga
    31021 tctgatcgcc atcgacttta ttgccttcat ggtcaaccat aatcacgcga tcgccgtcgc
    31081 cgtcgaaggc aataccgaga tccgcttttt cagccagcac acgagcctgg agcacgcgaa
    31141 cgtcggtagc ccccacttcg gcattgatgt ttacaccgtt tggctcacaa ccgatagcga
    31201 taacgttcgc ccccagttcg cgcagcacgt tcggcgcgat gtgataagtc gcaccgtttg
    31261 cacaatccac cacaatcttc agttcactga ggctaagttc gttcgggaac gtggctttgc
    31321 aaaactcgat atagcgaccc gcggcatcaa cgatacggct ggctttaccc agttctgccg
    31381 aatcaacgca gctgatctcc ttttccattt ccgcttcgat ggcctcttct accgcatccg
    31441 gcagtttagt gccgtcgata gagaagaatt taatcccatt atcgtagaac gggttatgcg
    31501 atgcagatat cacaattccg gcctctgcgc ggaaggtacg cgtcagataa gccacggccg
    31561 gtgttggcat cgggccagtg aagagtgcgg aaaggcccgc tgccgccaga cccgcttcca
    31621 gtgctgactc cagcatatag ccagaaatac gcgtgtcttt accaataata atcttacggg
    31681 agccgtggcg cgccagcact ttacccgcgg cccaacccag cttaagcaca aaatcaggtg
    31741 tgatcggcgc atcccctaca cgaccacgaa tcccatcggt accgaaatat ttacgattac
    31801 tcatagcgtt tgttttcctt tgcagacaga gtggcttcca ccacccgcat cgcttctacg
    31861 gtttctttga cgtcatgaac acgaatgatg tgcgcgcctt gcattgcggc aatgaccgca
    31921 caggccagac taccgctcag gcgctcggac ggccccacgt tcagcagctg cccaatcatc
    31981 gattttcgtg acatacccac caacagcggc aggttgaaat ggtgaaattc agccaggcgc
    32041 gccagtaatg aatagttatg ggagagattt ttaccgaaac cgaatccggg gtcgagcaac
    32101 aatttctctt ttgcgatacc cgcctgctcg caacgtgcta tttgctcaat aaagtagcga
    32161 ttcacttctg caaagacatc gtcatacttc ggagcttcct gcatggtttt tggatttccc
    32221 tgcatatgca tcagacaaac cggtaaaccg gtttctgcag ccgcctccag agcgccaggt
    32281 tcggaaaggg agcggatatc attaataatg tgagcgccaa ctttcgctga ctcacggatg
    32341 acttctggtt tggatgtatc gaccgagatc cagacttcga agcgttgagc aattgcctca
    32401 accacaggaa taacacgttg caactcttct tcaacgctaa cttccgccgc ccctgggcgc
    32461 gtggactcgc caccaacgtc aatgatcgtc gcgccagcat tgatcatcag attcgcatgt
    32521 ttcaccgcat ctatcagcga gttatgcgtg ccaccatccg aaaaggaatc aggcgtgacg
    32581 ttgaggatcc ccattacgtg aggatggcta aggtccagtg aagtaccctg ggcaaagagt
    32641 ttcatgatgt tatccctggt atgaattgat aagaaaaaac cccggagcac gccccggggt
    32701 tttcggtaca aatacagtca tctgatgcgg gaacttactt gtcgcctaac tgctctgaca
    32761 tggtgttacc cgggttcggc gtacgcggtt catcaaccgg acgaggagcc tttggactac
    32821 cattgtcgcc agaattgtta gaagcgcctg gttcttccca gcccgctggc ggacgtacat
    32881 cgcgacgtgc catcaggtca tcaatctgcg gtgcgtcgat agtctcatat ttcatgagag
    32941 catctttcat cgcatgcaga atatccatat tgtcggtcag aagctgacgc gcacgattat
    33001 agttacgctc aatcagtgct ttcacttcct ggtcgatgat acgtgcagtt tcatcggaca
    33061 tatgtttcgc tttcgctacg ctacggccga ggaatacttc accttcttct tccgcgtaca
    33121 gcagtggacc caatttctca gagaagcccc actgagtcac catgttacgt gccaggttgg
    33181 tcgcaacttt aatatcgttg gacgcaccgg tagaaacatg ttccggcccg tagatgatct
    33241 cttctgccag acgaccaccg tacagcgtag aaatctggct ttccagtttc tgacggctgg
    33301 cgctgattgc gtcgccctca ggcaagaaga aagtcacacc cagcgcacga ccgcgtggga
    33361 taatcgtcac tttatgcacc ggatcgtgtt ccggcaccag gcgaccgata atcgcatgac
    33421 ccgcttcgtg gtaagccgtt gattctttct gcgcttccgt catcaccatg gagcgacgtt
    33481 ccgcacccat catgattttg tctttcgctt tctcgaactc aaccatcgac acaacgcgtt
    33541 tgttgccacg agcagcgaac agtgccgctt cgttcaccag gttcgccagg tcagcaccgg
    33601 agaaaccagg agtaccacgg gcaatgattg ccgcgtcgat atcgggtgcc aatggtacgc
    33661 gacgcatgtg aactttcagg atctgctcac gaccgcgaac atctggcaag ccgaccacaa
    33721 cctggcggtc gaaacggcca ggacgcaaca gggccgggtc gagaacgtcc ggacggttag
    33781 tcgcggcgat aacgatgata ccttcgttac cttcgaagcc atccatctca accagcatct
    33841 ggttcagagt ctgttcacgt tcatcgtgac caccgcccag accagcgcca cgctggcggc
    33901 ctacggcgtc gatttcatcg ataaagatga tgcacggtgc cgctttcttc gcctgttcga
    33961 acatgtcacg aacacgggat gcacccacac cgacgaacat ttctacgaag tcagaaccgg
    34021 agatagtaaa gaacggaact ttcgcttcgc ctgcaatcgc tttcgccagc aacgttttac
    34081 cggtacccgg aggaccgacc atcaggacgc ctttcgggat cttaccgccg agtttctgga
    34141 agcggctcgg ctcgcgcaga tactctacca gttcagcaac ttcttctttt gcttcgtcgc
    34201 agcccgcaac gtcagcaaag gtcgttttga tctgatcttc cgtcagcatg cgcgctttgc
    34261 tcttaccaaa cgacatggca cctttgccac cgccgccctg catttgacgc atgaagaaga
    34321 tccagacacc aatcagcaac agcatcggga accaggagat gaagatagaa gccagcaggc
    34381 ttggttcttc aggcggttca ccgacaacct tgacgttctt ggtcaacagg ttatccagta
    34441 atttcggatc ctgaaccgga atgtaagtgg tataacggtt actatctttc ttggtaacgt
    34501 tgatttcacg tccgttgata cgcgcttcac gaacctggtc gttattgacc tcttgtagga
    34561 aggtagagta atccacctta cggccattag actcgctggg cccaaagctc tggaatactg
    34621 acatcagcac aacggcaatg accagccaga gtattaggtt tttcgccatg tcactcaagg
    34681 gattaacctc ttattacaac tgtgttaaaa acagcgtcag gatactctat atccagcatc
    34741 tttcaaactt tcgtctgaaa tctcccggtt agggtttacg cccggtcgct acaatataca
    34801 cttctcgcga acgtgcacga gaagagtccg gcttacgaac tttgaccttc gtaaacaggg
    34861 agcgaatttc ccttagatac tcatcgaaac cttcgccctg gaacaccttc actacaaaac
    34921 tgccacctgg cgctaataca tcacgacaca tttctagcgc cagttccacc agatacatgg
    34981 cacgggggat atccaccgcc ggtgttccgc tcatgtttgg tgccatatcg gacatgacaa
    35041 cctggacttt gctgtcgcca acgcgctcca gcagtgcttt catcaccagt tcatcacgaa
    35101 aatcgccctg aagaaagtcc acaccaacga taggatccat aggtaaaaga tcgcaagcga
    35161 tgatgcggcc tttgccgcca atttgggtga ccacatattg tgaccaacca cccggagcag
    35221 caccaaggtc gacaaccgtc attcccggtt taaagagttt gtcactttgc tgtatttcat
    35281 caagtttaaa ccaggcacgg gaacgtaatc cctttttctg tgcctgttga acatatttat
    35341 cgctaaagtg ttcctgaagc cagcggctgg agctggcaga acgcttctta cctgtcattt
    35401 aactttccca tggggataac tcatcgtaac caattgcgta aatttttacg cgcctatttg
    35461 gtgatatatg ggagatggcg gtagaatgac ccgttttcaa tcccaacgta agcaaaaaat
    35521 acgatgaatc tgagtactaa acaaaaacag cacctgaaag gtctggcaca tccgctcaag
    35581 ccagttgttc tgcttggcag taatggtttg accgaagggg tgctggccga gattgaacaa
    35641 gcgttagagc accatgaact catcaaggtg aaaatcgcca ccgaagatcg cgaaacaaaa
    35701 accttgatcg tggaagctat cgtgcgcgaa accggcgcct gtaatgtaca ggtcatcggt
    35761 aaaacgctgg tgctttatcg cccaactaaa aaacgtaaaa tctcgctgcc acgctaagat
    35821 tatcctaaag ttacacacat ttgctgtgta aaacgagggg ttttccgcag gcaggagagc
    35881 aaaatgccac gctctgttcg ttgataaaag gccgcagagc ggcctttttc ctttctttac
    35941 aatacatcaa catcttgagt attgggtaat tcttacaggt attccacctt aattacttca
    36001 aattctactt cgccgcccgg cgttttgatg accacaacat catcttcttc tttgccgatc
    36061 aggccacgag caataggcga gtttacagaa atcaggtttt gtttaaagtc agcttcgtca
    36121 tcgccaacga tgcgataagt ctgttcttcg tcagaatcca gattcagcac cgttacggta
    36181 gcaccaaaaa taacgcgccc attgttgggc attttggtga catcaatcac ctgcgcgttt
    36241 gacagcttgg cttcgatgtc tttgatacgg ccttcgcaga agccctgctg ttcacgagct
    36301 gcgtggtatt cggcgttttc tttcaggtcg ccatgctcac gcgcttccgc gatagcagca
    36361 atgatttcag gacggcgcac agatttcaga aaatccagct cttcgcgtaa tttttcagcg
    36421 ccgcgtaagg tcatcggaat agcttgcatt tgttatacct cttgaatatt cctgatgggg
    36481 caagtcttca cccaccccgg ctgttcggcc tgcccggcat aactcctcac cgggaccaga
    36541 agcaaaaaaa taccgacccg ggtacaagtc ccaggtcagc tacaattcac attttgatgg
    36601 tcattttacc ctgaagttcc cgaagggtca tcgtttactt tatagggcgt tgcgccgtag
    36661 tatgacggct cgattccagg ttgttagtgc gagattatgc gattttccag atttatcatc
    36721 ggattgacca gctgtatagc gttcagtgtt caggccgcga atgttgatga gtacgttact
    36781 caactccccg caggtgccaa ccttgccctg atggtgcaaa aagtcggtgc gtcggccccc
    36841 gctattgatt accacagtca gcagatggcg ctgcccgcca gtacccagaa agtgattact
    36901 gcgctggcgg cgttgattca actcggcccc gattttcgtt tcaccacgac gcttgaaacc
    36961 aaaggcaatg tggaaaacgg cgtacttaag ggtgacttag tggcgcgatt tggtgccgat
    37021 ccgacgttaa aacgtcagga tattcgcaat atggtcgcga ctttgaaaaa atctggcgtc
    37081 aaccaaatcg atggcaatgt gttgatagat acctccattt tcgccagcca cgataaagcc
    37141 cccggctggc catggaatga catgacacaa tgctttagcg ctccgcctgc tgccgccata
    37201 gttgaccgca actgtttctc cgtctcgctc tacagtgccc caaagcctgg tgatatggct
    37261 tttatacgcg tggcatctta ttaccccgtt acgatgttca gccaggtacg caccctcccc
    37321 cgtggttctg ccgaagcgca atactgcgaa ctggatgtgg tgccaggcga cctgaaccgc
    37381 tttacgctga cgggatgcct gccacaacgt tctgagccgc tcccgttggc ttttggcgtg
    37441 caggatggag ccagctatgc cggtgcaatt ctgaaagatg agttaaaaca ggcgggtatc
    37501 acctggagcg gaacactgct gcgccagact caggttaacg aacctggaac ggtagttgcc
    37561 agtaaacagt cggccccgct gcacgatctg cttaagatta tgctgaaaaa gtcggacaac
    37621 atgatcgccg atacggtttt ccgcatgatc ggccatgcgc gcttcaatgt gcctggaaca
    37681 tggcgggctg ggtcggacgc cgtgcgtcag atcctgcgcc agcaagccgg tgtcgatatt
    37741 ggtaacacca ttattgccga tggttcaggg ctttcgcggc ataacctgat tgcccccgcc
    37801 accatgatgc aggtgctgca atacattgcc caacacgaca atgaacttaa ctttatctcc
    37861 atgctgccgc ttgcgggcta tgacggctct ttgcagtacc gtgcaggtct gcatcaggcg
    37921 ggcgtggatg gcaaagtctc agcgaaaacc ggttcgttgc agggggtata taacctggcg
    37981 ggattcatta ccacagcgag cgggcaacga atggcatttg tacaatatct ttctggctat
    38041 gcagtaaaac ccgcggatca gcgtaatcgc cgtattccgt tagtgcgttt tgaaagccgt
    38101 ttgtacaaag atatttatca gaacaattag tcaaaaagaa accccggcgc atggccgggg
    38161 tttcagatta ttgacaaagt gcgctttgtt tatgccggat gcggcgtgaa cgccttatcc
    38221 ggcctacaaa atcgtgcaaa ttcaatatat tgcaatcatc ttgtaggcct gataagtgta
    38281 gcgcatcagg cttatttggc attggtcatt agtgattaac gcttgtaaat gaactcaacg
    38341 ccttcttcgt cgtcttcgtc ccagtcgtca tcccagtctt catcatcctc ttcagcaatc
    38401 tcttcaagct gctgacggtg ataatcatcc cacatgaatt cgactttctc tggctgtttc
    38461 gcttcttcag cctgcacgac cgggttttca atgataaagg tcatcacatc ccagcagaga
    38521 tctttcacgc ccagtccgct cgccgcagag atcagataat atttatcttc ccagcccagc
    38581 gcttcagcga tcgctttcgc tttctcttcg gcttctgcct tatccagcag atcgatcttg
    38641 ttgaagacta accaacgcgg tttcgccgcc agatcctggc tgtatttttc cagctcgctg
    38701 ataataatac gcgcgttttc aaccggatcg gtgccgtcaa tcggatcgat atcgatgagg
    38761 tgcaacagga cgcggcaacg ttccaggtgc ttcaggaagc gaatgcccag acctgcgcct
    38821 tccgcagcgc cttcaatcag tcctggaata tcggcaacaa cgaagctctt ttcgttgtcc
    38881 attcgtacca cacccagact tggcaccaga gtggtaaacg gataatccgc cactttcggt
    38941 ttagccgccg ataccgcacg aataaaggtc gatttacccg cgtttggcat ccccaacata
    39001 ccgacgtcag ccagcagcat cagctccagc agcagctcac gcttatcgcc cggcgtaccg
    39061 ttagttttct gccgtggtgt acggttaact gacgatttga aacgggtatt gcccagaccg
    39121 tgccagccgc ccttagcaac cagcagacgc tgaccgtgtt tagtcatatc acccatggtt
    39181 tcaccagtac cctggtcgat tacacgcgta cctaccggca ctttaatcgt cacgtcttta
    39241 ccgcgcttac cggtacaatc gcggcttgcg ccgttctgac cgcgctctgc acggaaagat
    39301 ttttcaaaac gataatcgat aagcgtgttc aggttctcgt cggcttccat ccatacgtca
    39361 ccaccatcac cgccgtcgcc gccatccggg ccgcctttcg gaatatactt ttcgcggcgg
    39421 aagctcacgc aaccattacc gccatcacct gcaacgacca gaatcgatgc ttcatcaaca
    39481 aacttcattt tattctccgt aaatcattcg cctgcgcggg gttgcgatac caccgttgta
    39541 tgcttacgta atccgcccca aatacgatga ccaatggcgg aatacatcgc gcccgcaacc
    39601 acgacaaacg caccgagata acctaaaagg tttaacatcg gtctggcgaa gaaatcgggc
    39661 caggccagtg ataaaagatc tgaaaaaaac agcgtaaaca gtggggtgag cgtgatgatc
    39721 gcgctcacct gagctgcctg ccagcgagcc atcgcttccg ccaaggcgcc atatcctacc
    39781 aaggtattca gtccgcaaaa aattaaacat gcgagctgcc agtggctaag ctgcgctatc
    39841 actccaggct ttgccagagg gaagagcgcg attgtacata aagtgtacag taaaaacagg
    39901 atctgcggtg aggccagccg acgcaataaa accttttgcg ccacgccata actcacccaa
    39961 accgtcgccg caccgacccc aaagataact ccccaggtgt aatcggtgag ctttgtaaat
    40021 atctcgacca gactggtgtt aaaaaacatc accagaccgc tcaggagcat caatgcccct
    40081 acaacctgag tgctgcgcat tttctctttc aggataaata cgctggcaac catcatgcca
    40141 actggcgaga gttgcccaat cacctgcgaa gcggtcggac tcaggtattg caaggatgag
    40201 ctgaacagga tgaagttccc aaacagcccg gcggtcgcca ctgccaacaa aatcaaccag
    40261 cgtggcttac gaaacacgcg taatggcggc aacctcttct tcaccgcaag aatggcacca
    40321 aggccaatac tcgccatcaa gaaacggtaa aacacgattg tcggaggttc catcacctcc
    40381 agcacctgct tcattgcgat tggcaacgcc ccccagcaaa ttgctgtggt aagcgccaaa
    40441 agaatgccaa tgcctgcctg ctgcttcata ccgtattccc tgcaaagcgc atttgcggat
    40501 taacgtcgca ccatgcgcat ttttaccggt tatcgaatgt aaaaagcccc gcaacgtgtt
    40561 gcggggcttt catccgttac cgggacgcga aaaacttatt cagcttcgat gctgataaat
    40621 ttacggtttt tcgggccttt aacttcgaat ttcactttac cgtctgcttt agcaaacaga
    40681 gtgtggtcac gaccgcaacc tacgttagcg ccagcgtgga atttggtacc acgttgacga
    40741 acgatgatgc tacccgccag aacggattcg ccaccgaaac gcttaacgcc caggcgttta
    40801 gcttctgaat cgcgaccgtt acgtgtggag ccgccagcct ttttatgtgc catttgaaat
    40861 ctctcctcag gtcttaggcg ctgatgccag taattttcac atcagtgaac cactgacgat
    40921 ggccctgctg cttacgatag tgtttacgac gacgaaactt aacgatttta actttctcgc
    40981 cacgaccgtg agcaacaact tcagctttga ttacgccgcc atcaacgaaa ggaacgccga
    41041 ttttgacttc ttcaccgttt gcgatcatca gcacttcagc gaactcaaca gtttcgccag
    41101 ttgcgatgtc cagcttttcc aggcgaacgg tctgaccttc gcttactcgg tgttgtttac
    41161 caccactttg gaaaaccgcg tacatataaa actccgcttc cgcgcacacc ttttcaatga
    41221 ttcaaagtgc gctataaata ttcacaatag ggcgcgaata ttacgcaaaa cgcacgcctt
    41281 tgacaagtgc tacagtcaat acacgaagaa aaaaaacaca acttgtacgg taacgtttat
    41341 ctgtgccatt ttttcagtac aattacccta tattcctaac cataaaccct aagttgcctt
    41401 tgttcacagt aaggtaatcg gggcgaaaag cccggctttt gcgatgaatt tagaaaaaat
    41461 caatgagtta accgcgcaag atatggcggg tgttaatgcg gcaatccttg agcagcttaa
    41521 ttccgacgtc caactgatca atcagttagg ctattacatc gtcagcggcg gcggtaaacg
    41581 tattcgtccg atgattgctg tactggctgc acgagctgtt ggctatgagg gaaatgcgca
    41641 tgtcaccatt gctgccctga tcgagtttat ccacacggcg actctgctac acgacgacgt
    41701 tgtggatgaa tcagatatgc gcaggggtaa agctaccgcc aacgccgcat ttggcaatgc
    41761 cgccagcgtg ctggtaggcg attttattta tacccgcgcg ttccagatga tgaccagcct
    41821 cggttcactc aaagtgctgg aagtcatgtc agaagctgta aacgtcatcg cagaaggtga
    41881 agttctgcaa ctgatgaacg ttaacgatcc ggacatcact gaagaaaact acatgcgcgt
    41941 tatctatagc aaaaccgcgc gtctgtttga ggctgccgcg cagtgttccg ggattctggc
    42001 tggctgtacg ccggaggagg agaaaggcct gcaggattat gggcgctatc tcggcactgc
    42061 tttccagttg atcgacgatt tactcgatta caatgccgat ggcgaacagt taggtaaaaa
    42121 cgtcggcgac gatctgaacg aaggtaaacc gacgctgccg ctgctgcatg cgatgcatca
    42181 tggcacacca gaacaggcac agatgatccg taccgccatc gaacaaggta acggtcgcca
    42241 tcttctggaa ccggttctgg aagcaatgaa cgcttgtgga tctcttgaat ggacgcgtca
    42301 gcgcgccgag gaagaagcag acaaagccat cgcagcgtta caggtgctcc cggacacccc
    42361 ttggcgagaa gcactcatcg gcctcgcgca catcgctgtt caacgcgatc gttaatcccc
    42421 tcccttcatc ccgcgcagtg cgcgggatga ttccagtaaa ttccataaaa acactaattc
    42481 agcgctataa cacctgaaaa aaagggcgat gaatctttat atattctgaa tattcacact
    42541 ctttacagga actttttagc gcaataggcc atcaggagta tagtgatgcc cgacagaaga
    42601 agtgttctga atgaaagcga ctaacttaag gagtgaggaa agatggaaag taatttcatt
    42661 gactggcatc ccgctgacat cattgcaggt ttgcgcaaga aaggaacgtc aatggcggcg
    42721 gaatctcgca gaaatggttt gagttcctca acgctggcaa acgcattatc gcgcccatgg
    42781 ccgaaaggag agatgattat tgcgaaagcc ctgggaactg acccctgggt tatctggcca
    42841 tcacgctacc atgatccgca gacccatgag tttatcgaca gaacgcagtt gatgcgtagc
    42901 tacactaaac cgaaaaaatg agtggtctgg cggtagcccc gcgaacgggg ctgccagctc
    42961 tcagacgatt actcgccttt cacacgctca atatttgcac ctaaagcgcg cagtttgtct
    43021 tcaatgcgtt cgtagccacg atcgatgtga taaatacgat caaccaccgt cgtcccttcc
    43081 gcaatacagc cagccagcac caggcttgct gatgcacgca gatcggttgc cataacctgt
    43141 gcaccagaaa gtttttcaac accatgacaa ataacggtat tgctttcgat ttcggcgtgc
    43201 gcgcccatac ggctcagctc tggcacatgc ataaagcggt tttcaaagac cgtttcggtg
    43261 atgaacccgg tcccttcagc caccaggttc aacagcgtga actgggcctg catatcggtc
    43321 gggaatgccg gatgcggcgc ggtacgtacg ttaacagcct tcggacgttt gccatgcata
    43381 tccaggctaa tccagtcttc gccgacttcg atgtccgctc cagcatcacg cagtttcgcc
    43441 agcacggcgt ccagagtatc tggctgcgcg ttacggcaga taattttgcc acgagagatc
    43501 gccgccgcca ccaggaaagt accggtttcg atacgatccg gcagcacgcg atagacaccg
    43561 ccgcctaaac gttccacacc ttcgatgacg atacgatcgg tgccctgacc gctaattttc
    43621 gcacccagcg taatcaggaa gttcgcggta tcgacgattt ccggttcacg cgctgcgttt
    43681 tcaataatcg tggtgccttc cgccagggtt gcagcacaca tgatggtcac cgttgcgcca
    43741 acgctgactt tatccatcac gatatgtgcg cctttcagac gaccatcgac ggaagcttta
    43801 acgtaacctt cttccagttt gatggtcgcg cctaattgtt cgaggccaga aatgtgtaga
    43861 tcaaccggac gagcaccgat cgtacaaccg ccaggtagtg aaacttgccc ctgaccaaag
    43921 cgcgctacca gcggccccag cgcccagata gaagcacgca tggttttaac cagatcgtaa
    43981 ggtgcgcaga atacattaac gtcgcgggca tcaatatgca cagaaccatt acgttctact
    44041 ttcgcaccca gctggcttag cagtttcatt gatgtatcga cgtctttcag tttcgggacg
    44101 ttctggatct ctaccggttc ttccgccagt agtgcggcaa aaagaatagg cagagcagca
    44161 tttttagcgc cggaaattgt gacttcgccc tggagcttcg ttggcccctg aacacgaaat
    44221 ttatccattt agtttgttct cagttaacaa ttcatatccg ctaccggcga atcgcccata
    44281 gctcaaaagc cgttcagttt gcgatcgcgc gcccactccg caggggtata cgctttgatc
    44341 gacacagcat gaatgcggtt atccgcaata tattccatca gcgggccata gaccgtctgc
    44401 tgttttttaa cccgactcat accgtcaaac aactcaccca cggcaataac ctgaaagtga
    44461 ctgccatcgc cggaaacgtg gacttcctgg agggagagag cgttcatcaa cacgctctga
    44521 atttcattat tttccatggg atcttcaatc atcagttaat aaaccagcga aacatcttag
    44581 agcaaagttg cgctggcata aataagcaaa aagcctcgct gataaatcag acaaggctcg
    44641 acttgcaggc aggtttgccg gacaggcggt taacgccata tccggcctga aaaaatttaa
    44701 cgaggcagaa catcagcagg caaattatac aatttcgcga gggtatacac tttgtcgttt
    44761 accccctgaa gcgtcacgtt gttgccctgc tttttcgcca gatcgataag atggagcagc
    44821 agtgccagtc cccccgtatc cacgcgggac acacggctaa gatcgatgca ggtaatcccc
    44881 ttcaccgctt cctcacgcat ttcccaaagc ggtagcagaa cgtcctgatc cagctctccg
    44941 gataacgcca gcgtgtcacc cgtctgcatc cagctcagtg actcgctcat tattttttct
    45001 cttccagagt gattttctgt tgagaaatcg atttcagttg cgcagtcagg ccgtcgatac
    45061 ctttggtacg cagcagcgtt ccccactcgt tttgtttggt ggtgatcata ctgacgcctt
    45121 cagcaatcat gtcgtaagct tgccagtttc ccgtctggga gtttttacgc cactggaagt
    45181 ccagacgcac cggcggacgg ccattcgggt caataatggt aacgcgaata ggcacaatgg
    45241 ttttatcgcc cagcggctgt tctggcgcaa tctgataggt ttgaccgtga tacatcgcca
    45301 gcgcctgacc gtaagcctgc ttcaggtact cacggaaagc ggcaaagtag gcttcacgtt
    45361 gagcaggggt cgcactcttg taatactggc ccagcaccag cgcaccggcg tatttcacct
    45421 gtacgtatgg cagcagttcc tgatcaacaa tggtacgcag ataatccggg ttggcccgaa
    45481 tttgcggttg ctcattcttc aggcgatcga acgttttctg cgccgcctcg tccatcagct
    45541 tataaggatt ggtctggtct gccgcggttg ccgcactcag aggtgcaatc accagcaaag
    45601 cgaccatcat taaacgttta aacatgcgtc ggttctcctg aaattatttc gttgtaccca
    45661 caggttcagt ggtttcatta ttacctggcg cagcagctgg cgcatcgcca ctattcttat
    45721 tgtcatcgcc tttactaccg taaaggaact gaccaatgag atcttccagc accatcgcag
    45781 acttggtgtc ctgaattgta tcgccatcct tcaggatagc agtccccagt tccgggtctt
    45841 caaaaccgac gtttaatgcc agatattgtt ctcccagcag gccggaagta cgaatgctca
    45901 gcgaactggt atcaggaatg tggttataac gttgttcaat ttccagcgtt acgcgcggca
    45961 gataggtttt cgggtccagc gtaatatccg ccacccggcc cacaacaaca ccaccaatgc
    46021 tgaccggaga gcgcgctttc aggccgccaa tgttatcgaa cgtcgcataa agcgtgtagg
    46081 tcgattctgt acgtatggac gtcacgttcg ccgccttcag gcaaacaaac agcgccgcca
    46141 gcagtgctgc taataaaaag atacccaccc aaatttcatt ttttttcgtt tgcatgaact
    46201 caattcccaa acatcaatgc ggtcagcaca aaatccagcc ccagaacagc cagagacgag
    46261 tggacaacgg tgcgagtggt tgcccggcta atcccggcag acgtcgggat ggcgtcgtag
    46321 ccgttaaaca acgaaatcca cgtcaccgtg atggcgaaca ccacgctctt aatcagacag
    46381 ttgaccagat ccatacgcca gtcgacggca ttttgcattg ccgaccagaa gaacccgcta
    46441 tcaatgcctt tccaactgac gccgaccagc gatccgcccc agatccccac ggcgacgaag
    46501 ataaccgtca acagtggtaa tgaaataacc ccagcccaga aacggggaga aataacccga
    46561 cgcagcggat ccaccgccat catctccata ctggagagtt gctctgtagc gcgcatcagg
    46621 ccgatttctg cggttaacgc cgaaccagca cgcccggcaa acaacagcgc ggcaaccacc
    46681 ggccccagtt cacgcagtag cgataacgcc accagcatac ccagactggt ttccgcgcta
    46741 taagtggtca gaaccagata accttgcagc cccaacacca ttccgatgaa cacgccagaa
    46801 actacaataa tcagcatcga caggacgccg acattataga gctggcgcac cagcagcggc
    46861 gcatgtttgc gaaattccgg tttgccgacc agcgcattga ataacattaa cccggcccgc
    46921 ccgaacgttc tcagggtttt aatcccttta tgtccgagcg acgccagcgc atttaacagc
    46981 atgagtggct taactccctg gtaaaagatc agcgtgataa tcgccggcag gatagcggaa
    47041 cggaacaggc ccgtcagcta tcccgtccag aaactgacgt acgcgcggat caggattcgt
    47101 ctgcaacgcc tgggcactgc catgagcgac aatttttttg tccgccagga tccaggcgtg
    47161 atccgcaata cttaacactt ccggcacatc gtgagaaacc accacacaag tcacgcccag
    47221 cgcgctgttc agctcagaaa tcagcttcac cagtacgccc atggtgatgg gatcttgccc
    47281 aacaaaaggt tcatcaaaca tgatgagatc cggctccagc gcaatcgcac gcgccagcgc
    47341 tgcacgccgc gccatcccac cggaaagttc agaaggcatt agtttagccg ctccacgcag
    47401 ccccacggcc tccagcttca tcatcaccgt actatgcaac aatggcgcgg gaagttgggt
    47461 atgttcgcgc agtggatagg cgacgttgtc aaatacgttc atatcagtga acaacgcccc
    47521 ggactgaaat aacatgctca tccgtttacg cactgtatac aggcgcgaac gagacatcgc
    47581 cggaatattc tcaccatcga aaaggatctc accatgatct ggtgcgattt gcccgccaat
    47641 cagacggagt agcgtcgttt taccgatgcc cgatggcccc atgatcgccg tgatcttccc
    47701 tcgcggcacg gtcagggaaa tattatcgaa gatgcagcga ttgccacgcg taaaactgac
    47761 atcgcgcata tcgactaaat tcgccacaga ctgctccata attcaccctt cgtcttgcgt
    47821 tgattttcta agcatggcgc tcaatttaac cttgaaccca acatatttac agaatattac
    47881 ccgccgtggt tagcgaaagc tggcatttgt tttacttttt agccgcataa agtcaaaatt
    47941 aagaatccgt tacggctttc tgaaaatctt cagcggaccg gcgagtatac ctgaagaaag
    48001 gacgttagat gcttttagct acggcactgt taattgttgg tttacttttg gtcgtttaca
    48061 gtgccgaccg cctggttttt gccgcgtcta ttctttgccg aacctttggc atcccgccgc
    48121 tgatcatcgg catgacggtg gtcagtattg gtacatcgtt accagaaatc atcgtctcgc
    48181 ttgccgcgtc tctgcacgaa caacgcgatt tagccgttgg tacagccctc ggctcaaaca
    48241 ttatcaatat attgctgatc ctcggtctgg ctgcgctggt tcgtcctttt accgtccatt
    48301 ccgatgttct gcgccgtgaa ttacccttaa tgttgttggt cagcgtggtg gccggttccg
    48361 tactctatga cggacaactt agtcgaagcg atggtatctt tctcctcttt ctggctgtgc
    48421 tatggctgct gttcattgtt aaacttgcac gtcaggggac tgacagcctg accagagagc
    48481 agcttgcaga gctgccgcgt gacggcggat tgcccgtcgc gtttttatgg ctcggcattg
    48541 cgcttatcat catgccagtg gccacgcgga tggtggttga taacgccacg gtgctggcga
    48601 attactttgc catcagcgag ttgacgatgg gtctgacggc aattgctatc ggaaccagtc
    48661 tgccggaact ggcaaccgca atagcggggg ttcgcaaagg tgaaaacgac attgctgtcg
    48721 gaaatatcat tggcgcaaac atttttaata ttgtcatcgt gttgggttta cccgcgctga
    48781 taacgccagg agagattgat ccactggcgt acagtcgtga ctacagcgtg atgttgctgg
    48841 tgagcattat ttttgcgttg ctgtgctggc ggcgctcccc gcaaccgggc cgtggtgtag
    48901 gggtattatt aactggcgga tttatcatat ggctggcgat gttgtactgg ttatcgccaa
    48961 tactcgttga ataactggaa acgcattatg tcgcacgtag agttacaacc gggttttgac
    49021 tttcagcaag caggtaaaga agtcctggcg attgaacgtg aatgcctggc ggagcttgat
    49081 caatacatca atcagaattt cacgcttgcc tgtgaaaaga tgttctggtg taaagggaaa
    49141 gttgtcgtca tggggatggg gaaatcgggg cacatcgggc gcaaaatggc cgcaacgttt
    49201 gccagcaccg gtacaccttc atttttcgtc catcctagtg aagccgcgca tggtgattta
    49261 ggcatggtca ccccacagga tgtggtgatt gctatctcta actcaggtga atccagcgaa
    49321 atcacggcct taattccagt gcttaagcgt cttcacgtac cgttaatctg catcaccggt
    49381 cgcccggaga gcagcatggc gcgcgccgca gatgtgcatc tgtgtgttaa agtagcgaaa
    49441 gaagcctgtc cgttagggct ggcaccgacc agcagcacca ccgccacgct ggttatgggc
    49501 gatgccctcg ctgtcgcgct gttaaaagca cgcggcttta ctgctgaaga ttttgcgctc
    49561 tcacacccag gcggcgcact gggtcgtaaa cttctgctgc gcgtaaacga tattatgcat
    49621 acgggcgatg agatcccgca tgttaagaaa acggccagtc tgcgtgacgc attgctggaa
    49681 gttacccgca aaaatcttgg tatgactgtc atttgcgatg acaatatgat gattgaaggc
    49741 atctttaccg acggtgattt acgccgtgtc ttcgatatgg gcgtggatgt tcgtcagtta
    49801 agtattgccg atgtgatgac gccgggggga atacgtgtgc gccctggcat tctggccgtt
    49861 gaggcactga acttaatgca gtcccgccat atcacctccg tgatggttgc cgatggcgac
    49921 catttactcg gtgtgttaca tatgcatgat ttactgcgtg caggcgtagt gtaaagattc
    49981 aaggataaac aacaatgagc aaagcaggtg cgtcgcttgc gacctgttac ggccctgtca
    50041 gcgccgacgt tatggcaaaa gcagagaaca ttcgtctgct gatcctcgac gttgatggcg
    50101 tactgtcaga tggcctgatt tatatgggca ataacggcga agagctgaaa gcgttcaatg
    50161 ttcgtgacgg ttatggcatt cgttgtgcgc tcacctctga tattgaagtc gctatcatta
    50221 ccgggcgaaa ggctaaactg gtagaagatc gttgtgccac attggggatc actcacttgt
    50281 atcaggggca gtcaaacaaa ctgatcgcct ttagcgattt gctggaaaaa ttggcgattg
    50341 ccccggaaaa tgtggcttat gttggcgatg atctcatcga ctgcccggta atggaaaaag
    50401 tgggtttaag cgtcgccgtg gccgatgccc atccactgtt gatcccgcgc gccgattacg
    50461 tgacgcgcat tgctggcggt cgtggcgcag tgcgcgaagt ttgcgactta ttactcctgg
    50521 cgcagggcaa actggatgaa gccaaagggc aatcgatatg agtaaagcca gacgttgggt
    50581 tatcattgtg ctatcactgg cggttctggt gatgatcggc attaatatgg ccgaaaaaga
    50641 cgataccgcc caggtggtcg tcaacaacaa tgatcccacc tataaaagcg agcatacgga
    50701 cacgctcgtc tataacccag aaggggcact aagctatcga ttgattgctc aacacgttga
    50761 atattattcc gatcaggccg tttcgtggtt tacgcagccg gtacttacca cgtttgataa
    50821 ggataaaatc ccgacatggt ccgtaaaagc agataaagcc aagctgacca atgaccggat
    50881 gctctattta tatggacacg ttgaagtcaa cgcacttgtg ccagactctc aacttcgcag
    50941 aatcacgacg gataacgcgc agatcaatct ggtgacgcag gatgttacct ctgaagacct
    51001 cgtcacgtta tacggaacaa catttaactc cagcggtctg aaaatgcgcg gcaacttacg
    51061 cagcaagaac gccgagctga ttgaaaaggt tagaacatcc tatgaaattc aaaacaaaca
    51121 aactcagcct taatcttgtg cttgccagct cacttctggc cgccagcatt ccggcatttg
    51181 ccgtaaccgg agacactgat cagccgatcc acattgaatc ggaccagcaa tctcttgata
    51241 tgcaaggcaa cgtggttacc tttaccggta atgtcatcgt cacccagggc accatcaaaa
    51301 ttaatgccga caaagtggtc gttacccgtc cgggcggcga acaaggtaaa gaagtgattg
    51361 acggatacgg taaaccggca acgttctacc agatgcagga caacggtaaa cccgttgaag
    51421 gtcacgcttc ccagatgcac tacgaactgg caaaagattt tgtcgttctg acgggtaatg
    51481 cttatctgca gcaggtcgat agcaacatta agggcgataa gatcacttac ctggtgaaag
    51541 agcagaaaat gcaggctttc agcgacaaag gcaagcgcgt aaccaccgtt ctggtgccgt
    51601 cgcagctgca ggacaaaaac aacaaaggcc agaccccggc acagaagaag ggtaattaat
    51661 tcgttatggc aacattaact gcaaagaacc ttgcaaaagc ctataaaggc cgtcgcgtgg
    51721 tagaagacgt cagcctgacc gtcaactccg gggaaattgt cggtctgctg gggccaaacg
    51781 gtgccggtaa gaccaccact ttctacatgg ttgtaggcat tgtgccgcgc gatgcgggca
    51841 acatcattat tgatgatgac gatatcagtc tgctgcctct gcatgcacgc gcgcgccgcg
    51901 gtatcggcta tctgccacag gaagcctcca ttttccgtcg cctcagcgtt tacgataacc
    51961 tgatggcggt actgcaaatt cgtgacgact tgtctgctga acaacgtgaa gaccgcgcga
    52021 acgagctgat ggaagagttt cacattgagc acctgcgtga cagcatgggg cagtcactct
    52081 ccgggggtga acgtcgccgt gtagaaattg cccgcgcact ggctgcgaat ccgaaattta
    52141 ttctgctcga cgaaccgttt gccggggttg acccgatctc ggttatcgat attaaacgca
    52201 tcattgaaca cctgcgcgac agcggcctgg gcgtgctgat cactgaccac aacgtgcgtg
    52261 aaacactggc ggtttgtgaa cgcgcttata tcgtcagtca ggggcatttg atcgcccacg
    52321 gcacgcctac agaaatctta caagacgaac acgttaagcg tgtatacctt ggggaagact
    52381 tcagactctg atagggtaga agtttgcgac gttttagcag gagagtacga ttctgaacat
    52441 gaagcaaggt ttgcaactca ggcttagcca acaactggcg atgacgccac agctccaaca
    52501 ggcaattcgt ctgttgcagt tgtcgacgct ggaacttcag caggagctac agcaggcgct
    52561 ggagagtaat ccgctgcttg agcaaatcga cactcatgaa gaaatcgaca cccgcgaaac
    52621 gcaagacagt gaaacgctgg acaccgccga cgcgctcgaa caaaaagaga tgccggaaga
    52681 gctgccgctc gatgccagtt gggacaccat ttacaccgct ggtacaccat ccggcaccag
    52741 cggtgactac attgacgacg agctgccggt ctaccagggc gaaacgacgc agaccttgca
    52801 ggattacctg atgtggcagg tcgagctgac accgttttcc gacactgacc gcgctattgc
    52861 tacctctatc gtcgatgccg ttgatgaaac cggttatctg actgtcccgc tggaagatat
    52921 tctcgaaagt atgggcgatg aagagatcga catcgacgag gttgaagccg tccttaagcg
    52981 gatccaacgg tttgatccgg tcggtgtagc ggcaaaagat ctgcgtgact gcctgctaat
    53041 ccaactctcc caattcgata agaccacgcc gtggctggaa gaggccagat tgatcattag
    53101 cgatcatctc gatctgttag ccaatcacga cttccgcact ttaatgcgcg tcacgcgtct
    53161 gaaagaagat gtgctgaaag aagccgtcaa tctgatccag tcgctcgatc cgcgccccgg
    53221 gcagtcgatc cagactggcg aacctgagta tgtcattcca gatgtgctgg tgcgtaagca
    53281 taacggtcac tggacggtag aactcaacag tgacagcatt ccgcgtctgc aaatcaacca
    53341 gcactacgcc tcgatgtgca ataacgcgcg caacgatggt gacagccagt ttatccgcag
    53401 caatctgcag gatgccaaat ggttgatcaa gagtctggaa agccgtaacg atacgctact
    53461 gcgcgtgagt cgctgtatcg ttgaacagca gcaagccttc tttgagcaag gtgaagaata
    53521 tatgaaaccg atggtactgg ccgatatcgc ccaggctgtc gaaatgcatg aatcgacgat
    53581 atctcgcgtg accacgcaaa aatacctgca tagtccacga ggcatttttg aactgaagta
    53641 tttcttttcc agtcacgtca ataccgaggg cggcggcgaa gcttcctcca cggcgattcg
    53701 tgcactggtg aagaaattaa tcgcggcgga aaacccagcg aaaccgttga gcgacagcaa
    53761 gttaacctct ttgctgtcgg aacaaggtat catggtggca cgccgcactg ttgcgaagta
    53821 ccgagagtct ttatccattc cgccgtcaaa ccagcgtaaa cagctcgttt gacccaaccg
    53881 ataaggaaga cactatgcag ctcaacatta ccggaaataa cgtcgagatc accgaggcac
    53941 tgcgcgaatt tgttacagct aaatttgcca aacttgagca atattttgac cgaatcaacc
    54001 aggtctatgt tgttctgaaa gtggagaaag tcacccacac ctcagatgca acactgcatg
    54061 taaacggtgg tgaaattcat gccagcgcgg aaggtcagga catgtacgct gccattgatg
    54121 gcttaattga taagctggca cgccagttga ccaaacataa agataaactg aaacaacact
    54181 aattgtccgg gcaattagca tgtgcatggc ggcctgttgt gcggcacaac gggccatttg
    54241 tacggttaat gctccgagcc tgttccactg tttgagtggg caggttctta ggtgaaatta
    54301 tgacaaataa tgatacaact ctacagctta gcagtgttct taacagggaa tgtacgcgaa
    54361 gccgcgtcca ctgtcagagc aaaaaacgcg ccctggaaat catcagcgag ctggcggcga
    54421 aacaacttag cctaccgcct caggtggttt ttgaagctat cctgacgcgg gaaaaaatgg
    54481 gcagtaccgg tatcggcaat ggtattgcca ttccgcatgg caaacttgaa gaagatactc
    54541 tgcgtgccgt tggcgttttc gttcagctcg aaacgcctat agctttcgat gccatcgaca
    54601 accaaccggt ggacttactt tttgccctgc tggtgccggc agaccaaact aaaacgcacc
    54661 tgcatactct gtcgctggtg gcgaaacgtc tggcggacaa aaccatctgc cgccgtttac
    54721 gcgcagccca gagcgatgaa gagctgtatc aaatcattac ggataccgaa ggtactccgg
    54781 atgaagcgta gttattcggt aatgtctctt ttagacgttg tgaggagaaa cagtacatgg
    54841 tactgatgat cgtcagcgga cgttcaggtt caggtaaatc tgtcgccctg cgtgcgctgg
    54901 aagatatggg tttttactgc gtggataacc ttcccgtagt gttgttaccc gatctggctc
    54961 gaactctggc cgatcgcgag atttctgccg ccgtcagcat tgatgttcgt aatatgccgg
    55021 agtcaccaga aatattcgaa caggcgatga gtaacctgcc tgacgctttc tcaccgcaac
    55081 tactgttcct ggatgccgac cgtaatacct taattcgtcg ttacagtgac acgcgccgac
    55141 tgcatccgct ttccagcaaa aacctgtcgc tggaaagtgc tatcgacaaa gaaagcgatt
    55201 tgctggagcc tctgcgttcg cgagcggatc tgattgtcga tacctcagaa atgtccgttc
    55261 acgagctggc tgaaatgctg cgtacccgtc tgctgggtaa acgtgaacgc gaactgacca
    55321 tggtctttga gtctttcggc ttcaaacacg gtatccctat cgatgcagat tacgtctttg
    55381 acgtgcgctt cttgccgaac ccgcactggg atccgaaact gcgtccaatg acaggtcttg
    55441 ataaacctgt cgccgcgttc ctcgaccgcc acacagaagt acacaatttt atctaccaga
    55501 cgcgaagcta tcttgagcta tggttaccta tgctggaaac caacaaccgt agctacctga
    55561 cggtcgccat tggttgtacc ggcgggaagc accgttcggt gtatattgca gagcaactgg
    55621 cagactactt ccgctcgcgc ggtaaaaacg tccagtcacg ccatcgtacg ctggaaaaac
    55681 gtaaaccatg accgtcaagc aaactgttga aatcacaaac aagctgggca tgcatgcccg
    55741 gcctgcaatg aagctgtttg aattaatgca gggttttgac gctgaagtgc tgttacgtaa
    55801 tgacgaaggc accgaggctg aagccaacag cgttattgcg ctgctgatgt tggattctgc
    55861 caaaggacgg cagattgaag ttgaagcgac cggtccacag gaagaggaag cactggccgc
    55921 cgttatcgcc ctctttaatt ctggttttga tgaagattaa tcttcatcac tttgacatac
    55981 aactcccttc aagctccccc gacaataaga aaatcacgta ctgaaatcgt tctcaatcaa
    56041 cgtcatttgt acattttgtg cgcttttcac tttcagaaga accttaagaa aaccttaaga
    56101 ggcattgttt aggttttgtt taagttaatc gaccatactg gagatcgtca gaaaatattt
    56161 ccaggagatg gcatgattcg cttatctgaa caaagtcccc tgggcaccgg aagacatcgg
    56221 aaatgttatg cgcatccgga agatgcccaa cgctgtatca agattgtcta ccatcgtggc
    56281 gatggtggcg ataaagagat ccgccgtgag ttaaagtact acgcgcatct tggtcgccgg
    56341 ttaaaagact ggagtggaat accgcgctat cacggtaccg ttgagacgga ttgcggaacc
    56401 ggttatgtct acgatgtgat tgccgatttt gacggcaagc cttccattac gctgaccgaa
    56461 tttgctgaac aatgccgtta cgaagaagac attgcacaac tgcgccagct actgaaacag
    56521 ctgaagcgtt atttgcagga taaccgtatc gtgacgatgt cgctgaagcc gcagaatatc
    56581 ctctgtcacc gcatcagtga gtcagaggtt atcccggtcg tctgcgacaa cattggtgaa
    56641 agcacattga ttccactggc gacctggtcg aagtggtgtt gtctgcgtaa gcaagagcgg
    56701 ttatggaaac gatttatcgc ccaaccggcg ctggctattg ccctgcagaa agatctgcaa
    56761 cctcgtgaga gcaaaacgct ggccctcact tcgcgcgaag cttaatccag ctggtgttgt
    56821 tgcataaacg gctcaccgcc taactgatac atctgccgta aaatccacgc ctgacggcta
    56881 cgcacgtagc cgtcaggcgt ggagactttg aaacgaagtg gattaggtaa tacagctgcc
    56941 agtaatgcag cttccgaccg ggtaagtttg ctcgcgggtt tgtggaaata acgttgtgcc
    57001 gcagcttcga cgccaaacac accgtcgcca aattcggcga tattcaggta aaccgtcagg
    57061 atacgctttt tgctccagac tgtttctatc cccagcgtta atccagcttc cagccctttt
    57121 cgtacccagc tacgcccatc ccataaaaag agatttttgg ccgtctgctg agaaatcgtt
    57181 gaagcaccgc gaatacggtt ttcattgcgc tcgttgtgcg ccagggcttt ctcaatggaa
    57241 gcgacatcaa agccccagtg ctcaggaaat ttctgatctt ctgcggcaat caccgccagt
    57301 cccatccacg gcgagatttg atccatactg acccagtcag aatgtgccac gtaacgaaaa
    57361 ttgccatgca gccaggcgct gacctgtcgc tcgaccatta ccgctgagaa gggaacaggc
    57421 gcaacgctaa acaacgcgat gcccccgccc cagaaaacgg cgaggacaac cattaaccgc
    57481 aaaagaaaac ggcgaacgaa actaaacacc gttaagcggc ttttactcat tcagccagaa
    57541 ccagcacgcg ggaaaccagc ttatcaatgc cgctcgccgc ttctgcaatg ttctgcgcca
    57601 gcatatatgc tggggtggtg acaatcttat tgtcttcatc aaccacgata tcatcgacag
    57661 gacacggcac atgctccgcg cccatctctt ccagtacttc tgcggtatcg atatcagtac
    57721 cgatggtcaa acgcagcggg aaatcgaaaa ttttcggcag catcgccggg gcaatacaca
    57781 taaaaccaag cggttttccg gcctgatgca tcgcttgtgc cagcgccttt aattcacggt
    57841 caacggtgca ttcgctacca agactggcaa aattgcttaa attcttcgcc gcgccaaccc
    57901 cccccggcac aatcaacgca tccagttcag cggcatcggc ctgggccaga ggacggattt
    57961 caccgcgcgt tattcgtgcc gcttcaatca gcacattgcg cgtttccgtc atcgcttcgc
    58021 cagttaaatg gttgataaca tcaacctgct gcttatccgg tgcaaagcag accgcctgcg
    58081 caccgctgcg tgaaatagct aacaacgtca acaccgcttc atgaatttca gaaccgtcat
    58141 agacgccgca tccgctcaga attacgccaa ttttcttcat tgtgatcatc ctttcgcaat
    58201 tgactgaaac acattaattt ttttaataaa aatggtacgc atcacacatt taactgattc
    58261 atgtaacaaa tcatttaagt tttgctatct taactgcgtg cggcctgaaa aacagtgctg
    58321 tgcccttgta actcatcata ataatttacg gcgcagccaa gatttccctg gtgttggcgc
    58381 agtattcgcg caccccggtc tagccggggt cattttttag tggcttttgc cacccacgct
    58441 ttcagcactt ctacgtcgtg acgccactct tctttcatct cttcaatcca ttcaccgacg
    58501 ttatcttccc aggcgggaag gtcaggagac tgaatttgct gacccagctg ttgcagatgg
    58561 cgtaacccca ctgaccccgc cgcaccttta attttatgtc cttcctcaac aatgcctttt
    58621 ttatcctgcg ccgtcagatt cgactccagc acgctgacat agcccggcat catcttctca
    58681 aacaccgcta acccgtcggt gatcagcttc ggtcctacaa gttcgagata ctgttccagc
    58741 atgggaatat cgagcaatgc ttctgattta ctgttctctt ctgtcgtcac cgtactcttc
    58801 tcatcatcct gggtatccca gaatttcttg atcatcgcgg ttagcgccgg aaccgaaagc
    58861 ggcttactca gcacatcatc cattccagca ttgaggtact cttgtttgtc tttcagcacg
    58921 ttagcggtta aggccaccag cggcggtaaa tcctcgcgcg gataacgctt cgtcagttca
    58981 cgagagatat ccagcccggt catatctggc aactgaatat ccagcaacac caggtcgtat
    59041 tcgcccggtt taaacatctc cagcgccgcc ttgccggtca tggcgacatc aacgctgtta
    59101 cctaattttt ccagcacaga acgcgcgaca atcacgttca gttcaatgtc ttccaccagc
    59161 agcacattca gcgccggtaa aggcatatcg tcttcatcaa acgcatcatc gacctcttct
    59221 gccaccgacg gtgcgtggat cgtcaacgta aaggttgaac ctttgccctg ttcgctggta
    59281 accgtaatat cgccgcccat atttttcgcc agacgacgag aaacggccag accaataccg
    59341 gtgccggtgg caggtttacc gccatgactg tctttcacct ggtaatacat ggcgaagatt
    59401 ttatccagct catcctgcgg aatgccgata ccagaatctt ccacttcaaa atgcagcata
    59461 tcgccttcat cgtagcgcac gcgcacggta acctggcctt gctgggtgaa tttgacggcg
    59521 ttactgatga ggttccacag gatctgccgt aaacgcgtcc cgtcggtaat gacctgatgc
    59581 ggtaatggca gcgtaggctc caggttaaag cgcaatcctt tttgttgcgc ctgcaatgcg
    59641 gagagatttt ccagatcggc aaggaagctg gtgaaatcaa ccggctgatt atcaagctgg
    59701 accttgcgcc gttccatctt atccatgtcg ataatatcgt taaagatatt ccccagcgtg
    59761 acggccgaaa catggatagt tttgagatat ttttcctgct cggcggtgag ttcggtatcc
    59821 agcagaatgc ggctcaggcc gacgataccg ttcagcggtg tacgcaattc gtgactgatg
    59881 gtggaaataa acgtcgtttt gtcgcggctg gcgcgttcaa gcgcatcctg ataccgctta
    59941 cgctcggtaa tgtcgcgacc aaagcccatc aaaccgtgac gtttacccac gcggtcgtag
    60001 tacggcactt tacggatttc aaagcaggct ttgcgcccgt ccgggtaatc cagccactgt
    60061 tcataggtca gtgacacatt atgacggaac actttttcat cggtttcaat gacttttgcg
    60121 gcggcttccg gtgagtaaac atcagcaggt ttcagatgaa ccagttgttt ttcgcttttt
    60181 ccggtcagca gctccatcgc gcggttacag ccggaaaact ctttatcttc gttacgataa
    60241 aaaaccaggt cgggtgaagc atcaaggaag gaacgtaaga atgaggattg ctgctcgagc
    60301 tgaatttgtg tctcttcgcg ctctttgatt tcaattttca gttggccgaa ggtttcctgc
    60361 agttctgctt ccgctttttc acgaacggca atttcctgat ttagctgggc aatattatct
    60421 tttaactgaa cgttgaggct caaatcgcgc tcgcgcatct cctccagttt ttgcaccagc
    60481 cgtgacagac gttgtcgtga ctcctccagt tgctcgacga ccaccgatag aaagtagacc
    60541 gcccacggcg taatcagcaa accaaagaag atagaacgaa taacatcaat gctttcgacc
    60601 tgaccatgca gcaccatggt taccgccatt tgtaccacaa tggcaagaac gacgagggcc
    60661 agcgccagca acattgagaa gcgcaccaga cctaacttca tcatcaggtc aacataatac
    60721 tgcgccagca gacgaatttg cttcattagg gaattccttc acgacaacct gccccaataa
    60781 tacccaattc tgacagttac gttataaatt gtgtgagaaa tgcggaattc ccctcaacga
    60841 cctactccgt tggaggtagc cacggacgtc ccagcgccga tccctgtacg ccatgttcat
    60901 tcagatagcg atccagctcg accattcccg tccatcgatt ttcgcaccac agcggagcaa
    60961 gcagcgtcgg gcgacgggcg ctggcggaaa tgcggtggta gatcacttcc ggtggcgtat
    61021 ggcgaatcat ttctccggca gtgagcgtat aatcctccag ttcaataccg ttcaaacgcc
    61081 ccgcttccca ggctttcgcc ataatgctgc ctttcacaat atgcagcgga tgcagcttta
    61141 tgccgtccac gctggtttca accactcgct ccagcgtttg caaacattca gcctgccctt
    61201 cgccaggcag gccgacaatt aagtgggaac acaccttcag cccgcgctga cgtgccagct
    61261 gggttgtacg ctgataacag gcaaaatcat ggccgcggtt gatgcgatgc agtgttttgt
    61321 cgtgggcggt ttgtagcccc agctccagcc acacttcgta accctggtcc ttatattcgc
    61381 aaagcagatc cagcaccgca tccggcacgc agtccgggcg ggtaccaaca cacaaaccga
    61441 caatattggc ctggctcacc gcctgctgat acatagaacg cagcacctga acttccgcaa
    61501 aggtgctggt atacgcctga aagtaggcca gatagcgttt agcgcggtta actaaattcg
    61561 cctggtgcgc cagttgctcg gcaatggaac gatgctgctg cgcttcatcg gcaaacgagg
    61621 caacattaca gaatgtgcag ccaccacgcc cgatggtacc gtcacggtta gggcagctaa
    61681 aaccgccgtg cagcgtcagc ttatgcacct tttgcccata acgacgggtg agatcaccac
    61741 caaacatatt gactaatttc tgtaactgca taatctgata gaccgcgcct tgaaaagagg
    61801 ccaaagcctg ccatttttag ctcaattcgg cgatgaccta gatcaatcgt cccgccctgc
    61861 ttttatcaac tgcataatca atcaaaatta ccgaaatttc atgcataatc acataaatca
    61921 cttttgctta tcttgtgtca gattttttta tctccttatg gattttaggc aaaaacagta
    61981 gcatgaaacg tcattaccaa ttaaggcagt ataaaatgct ggttttctcg tcagttcaag
    62041 gcaggataag ggttaacacg cctttatgac agtcaggaat tgactgtttc tctaacgact
    62101 ctccttttag ccttaaagat aaaatctatt ttaatttcag ccatttaata aagaattttg
    62161 cgctaaagca catttctgta ccaataagct tgccatttga cctgtatcag ctttcccgat
    62221 aagttggaaa tccgctggaa gctttctgga tgagcagcct gctcatcata tttatgcagt
    62281 aattgagatc ccctcttcac cgtattaacc gatgcgaaaa ggacaacaag ggggcgaatg
    62341 cgaggcgcgc gtatgacacg caaaccccgt cgccacgctc tttctgtgcc cgtgcgcagc
    62401 ggttcggaag tggggttccc gcagagcctg ggggaggttc acgatatgtt gtacgataaa
    62461 tcccttgaga gggataactg tggtttcggc ctgatcgccc acatagaagg cgaacctagc
    62521 cacaaggtag tgcgtactgc aatacacgca ctggcccgca tgcagcaccg tggcgcgatt
    62581 ctcgccgatg gtaaaaccgg cgacggttgc ggcttgctgt tacaaaaacc ggatcgcttt
    62641 tttcgcatcg ttgcgcagga gcgcggctgg cgtttagcaa aaaactacgc tgtcgggatg
    62701 ctcttcctga ataaagatcc tgaactcgcc gctgccgcac gccgcatcgt tgaagaagaa
    62761 ctgcaacgcg aaaccttgtc gattgtgggc tggcgtgatg tccccactaa cgaaggcgtg
    62821 ctgggtgaaa tcgccctctc ctctctgcca cgcattgagc aaatttttgt gaacgccccg
    62881 gcaggctggc gtccacgcga tatggagcgc cgtctgttta tcgcccgccg ccgcattgaa
    62941 aagcgtctcg aagccgacaa agacttctac gtctgtagcc tgtcgaatct ggtgaacatc
    63001 tataaaggtc tgtgtatgcc ggcggatctg ccgcgctttt atctggatct tgcggacctg
    63061 cgtctggaat cggccatttg cctgttccac cagcgcttct ccactaacac cgtaccgcgc
    63121 tggccgctgg cgcaaccgtt ccgctatctg gcgcataacg gtgaaatcaa caccatcacc
    63181 ggtaaccgcc aatgggcgcg tgcgcgtacc tataaattcc agacgccgct gatccctgac
    63241 ctgcacgacg ccgcgccatt cgtcaacgaa accggttctg actccagttc catggataac
    63301 atgctggaac tgctgctggc aggcgggatg gatatcatcc gcgccatgcg cctgttagtg
    63361 ccacccgcct ggcagaacaa cccggatatg gacccggaac tgcgtgcctt ctttgacttt
    63421 aactccatgc atatggagcc gtgggatggt ccggcgggca tcgtgatgtc cgatggtcgc
    63481 ttcgctgcct gtaacctcga ccgtaacggt ctgcgtccgg cacgctacgt cattaccaaa
    63541 gacaagctca tcacctgcgc ctctgaagtc ggtatctggg attaccagcc tgacgaagtg
    63601 gtcgaaaaag gccgcgtcgg gccaggcgaa ctgatggtga tcgacactcg cagtgggcgt
    63661 attctgcact cggcagaaac cgatgacgat ctgaaaagcc gccaccctta taaagagtgg
    63721 atggagaaaa acgtccgccg actggtaccg tttgaagatc tgcccgatga agaagtgggt
    63781 agccgcgaac tggacgacga cacgcttgcc agctaccaga aacagtttaa ctacagcgcg
    63841 gaagagctgg actccgtaat tcgcgtactg ggcgaaaacg gtcaggaagc ggtcggttcg
    63901 atgggcgatg ataccccatt cgccgtgctc tccagtcagc cgcgcattat ttacgactac
    63961 ttccgccagc agtttgccca ggtgactaac ccgccaatcg acccgctgcg tgaagcgcat
    64021 gttatgtcgc tcgccaccag tatcggtcgt gaaatgaacg tcttttgcga agcagagggc
    64081 caggcgcacc gtttaagctt taaatcgccg attctgctct actccgattt caaacagctc
    64141 acgacgatga aagaggagca ctaccgcgca gatacgctgg atatcacctt tgacgtcact
    64201 aaaaccacgc tcgaagcgac agtcaaagag ctatgcgaca aagccgaaaa aatggtacgt
    64261 agcggcaccg tgttgctggt gctctccgac cggaatatcg ctaaagatcg cctgccggtt
    64321 ccggctccga tggcggttgg cgcgatccag acccgtctgg tcgatcaaag cctgcgttgc
    64381 gatgccaaca tcatcgtcga aaccgccagc gcccgcgatc cgcaccactt cgccgtgttg
    64441 ctgggtttcg gcgcgacggc tatttatccg taccttgcct atgaaacgct gggccgcctg
    64501 gtagacaccc acgcgattgc caaagattat cgtaccgtga tgctcaacta ccgtaacggc
    64561 atcaacaaag gcttgtacaa aatcatgtcc aaaatgggca tctccaccat cgcctcttac
    64621 cgctgctcga aactgtttga agcggtcggt ctacacgatg atgtagtggg cctgtgcttc
    64681 cagggcgcgg tcagccgcat tggtggggca agctttgaag acttccagca ggatctgctg
    64741 aatctgtcga aacgtgcctg gctggcgcgc aagccgatta gccagggcgg cctgctgaaa
    64801 tacgtccacg gcggcgaata ccacgcctac aacccggacg tggtgcgcac gctgcaacag
    64861 gcggtacaaa gcggcgagta cagcgactat caggaatacg cgaagctggt taatgagcgt
    64921 ccgacaacca cgctgcgcga tctgctggca attacgccgg gtgaaaacgc ggtcaacatt
    64981 gctgatgttg aaccggcaag cgaactgttt aaacgctttg ataccgccgc gatgtctatc
    65041 ggcgcgttaa gcccggaagc ccacgaggcg ctggcggaag cgatgaatag catcggcggt
    65101 aactcgaact ccggtgaagg cggcgaagac ccggcgcgct atggcactaa caaagtgtcg
    65161 cgcatcaagc aggtagcttc tggtcgcttc ggcgttactc cggcgtatct ggtcaatgcc
    65221 gacgtcattc agattaaagt cgcccagggc gcgaagccgg gcgaaggcgg tcagttgccg
    65281 ggcgacaaag tcactcctta catcgccaaa ctgcgctatt cggtgcccgg agtgacgctg
    65341 atctccccgc cgccgcacca cgatatctac tctatcgaag acttagcgca gcttattttc
    65401 gacctcaagc aggttaaccc gaaagcgatg atctccgtta agctggtgtc cgaaccaggc
    65461 gttggcacca tcgcgactgg cgtggcgaaa gcttatgcgg acttgatcac catcgcgggc
    65521 tatgacggcg gcaccggcgc aagcccgctg tcgtcagtga aatatgcagg ctgcccgtgg
    65581 gaactcggtc tggtggaaac ccaacaggcg ctggttgcca acggcttgcg tcataaaatt
    65641 cgtttgcagg ttgatggcgg cctgaaaacg ggtgtcgata tcatcaaagc ggcgattctc
    65701 ggcgcagaaa gcttcggctt cggcactggc ccaatggtgg cgctcggctg taaatatctg
    65761 cgtatttgcc acctgaacaa ctgcgccacg ggtgtagcaa ctcaggatga caaactgcgt
    65821 aagaatcact atcacggcct gccgttcaag gtgactaatt actttgagtt tatcgcccgt
    65881 gaaacccgcg agctgatggc acagctggga gtaacgcgtc tggtggatct gattggtcgc
    65941 accgacctgc tgaaagagct ggacggtttc accgccaaac agcagaaact ggcgctgtcg
    66001 aagctgctgg agactgccga gccacatgca ggtaagtcac tctactgcac tgaaaacaac
    66061 ccgccgtttg ataacggcct actgaacgcg cagttgctgc aacaggcgaa accgtttgtc
    66121 gatgaacgcc agagcaaaac cttctggttc gatatccgca acaccgaccg ttctgtcggc
    66181 gcgtcgcttt caggctatat cgcccagacg cacggcgatc aggggctggc agccgatcct
    66241 atcaaagcgt acttcaacgg caccgcaggc cagagtttcg gtgtgtggaa cgcgggcggc
    66301 gtggaactat acctgaccgg ggatgccaac gactatgtcg gtaaaggcat ggcgggcggc
    66361 ttaatcgcca ttcatcctcc ggttggttcc gccttccgca gccatgaagc aagcattatc
    66421 ggcaacacct gcctgtatgg cgcgaccggt ggtcgtctgt atgccgcagg ccgcgcgggt
    66481 gaacgtttcg gcgtgcggaa ctccggtgct atcaccgtgg tagaaggcat tggcgacaac
    66541 ggctgtgaat atatgacggg cggtattgtc tgtattctgg gtaaaaccgg cgttaacttc
    66601 ggcgcgggca tgaccggcgg cttcgcttac gttctcgatg aaagcggtga tttccgcaaa
    66661 cgcgttaacc cggaactggt cgaagtctta agcgtcgacg atctggcgat ccatgaagag
    66721 catctgcgcg gtcttatcac cgagcatgtt cagcataccg gttctcagcg cggtgaagag
    66781 attctggcga actggtcaac cttcgccacg aaattcgcgc tggttaaacc gaagtccagt
    66841 gatgtaaaag cactgctggg tcaccgtagt cgtagcgcag cagagttgcg cgtgcaggcg
    66901 cagtaagggg tagcaacaat gagtcagaat gtttatcaat ttatcgacct gcagcgcgtt
    66961 gatccgccaa agaaaccgct gaagatccgt aaaattgagt ttgttgaaat ttacgagccg
    67021 ttttccgaag gccaggccaa agcgcaggct gaccgctgtc tgtcgtgcgg caacccatac
    67081 tgcgagtgga aatgcccggt acacaactac atcccaaact ggctgaagct cgccaacgag
    67141 gggcgtattt ttgaagcggc agaactgtcg catcagacca acacactgcc ggaagtatgc
    67201 ggacgtgtct gcccgcaaga ccgtctgtgc gaaggttcct gcaccctgaa cgatgagttc
    67261 ggtgcggtga ccatcggcaa cattgagcgt tatatcaatg ataaagcgtt cgaaatgggc
    67321 tggcgtccgg atatgtccgg cgtgaagcaa accggtaaaa aagtggcgat tatcggtgca
    67381 ggcccggcag gtctggcgtg tgcggatgtc ctgacgcgta acggcgtaaa agccgttgtc
    67441 ttcgaccgtc atccagaaat tggcgggctg ctgaccttcg gtattccggc cttcaagctg
    67501 gaaaaagagg taatgacgcg tcgccgtgaa atcttcaccg gcatgggtat tgaattcaaa
    67561 ctcaataccg aagtgggccg cgacgtacag ctggacgatc tgctgagtga ttacgatgcc
    67621 gtgttccttg gcgttgggac ttatcagtca atgcgcggcg ggctggaaaa cgaagacgcc
    67681 gatggcgtgt acgcagcgct gccgttcctt atcgccaaca ccaaacagtt aatgggcttt
    67741 ggtgaaaccc gcgacgaacc gttcgtcagc atggaaggca aacgcgtggt agtacttggc
    67801 ggtggcgaca ctgcgatgga ctgcgtgcgc acgtctgtac gccagggggc aaagcacgtt
    67861 acctgtgcct atcgtcgtga tgaagagaac atgccgggtt cccgccgcga agtgaaaaac
    67921 gcgcgggaag aaggcgtaga gttcaaattc aacgtccagc cactggggat tgaagtgaac
    67981 ggtaacggca aagtcagcgg cgtaaaaatg gtacgtaccg aaatgggcga accggatgcc
    68041 aaaggccgtc gccgcgcgga gatcgttgca ggttccgaac atatcgttcc ggcagatgcg
    68101 gtgatcatgg cgtttggttt ccgtccacac aacatggaat ggctggcaaa acacagcgtc
    68161 gagctggatt cacaaggccg catcatcgct ccggaaggca acgacaacgc tttccagacc
    68221 agcaacccga aaatctttgc tggcggcgat atcgtccgtg gttccgatct ggtcgttacc
    68281 gccatcgccg aaggtcgcaa agcggcagac ggcattatga actggctgga agtttaagct
    68341 ctgtcccagc gccgcctcct cagaaaagag tcggcgctga aattctcctt tctttctgga
    68401 aatcgcctcg cataatctga agaaaaccaa tgtaatacgc tcatctggat tgttaccggt
    68461 agatgcccgg cgtatagctg attccattat tcaacgcagc gccatataaa gggacagata
    68521 atggaatcgc tctcagagag aacctcaaca ggctaccagc aaatccacga cggcattatt
    68581 catctggtcg atagcgcccg aacggaaacg gtacgtagcg ttaacgcgct gatgaccgcg
    68641 acgtactggg aaattggccg acggattgtc gaatttgaac aaggtggtga ggccagagca
    68701 gcttatggtg cacagttgat tgatcggcta tcgaaggatt taagtcgacg gtataaacgt
    68761 ggggtctctg caaaaaactt acgccaaatg aggctttttt acctcttttt tcaacatgtt
    68821 gaaattcgcc agacagtgtc tggcgaatta acaccattgg gaattccgca gacaccatct
    68881 gcagaattcc catcggcaaa gatttggcag acgctgtctg ccaaattgag aggctgtttg
    68941 atatttccac agcttttgta ttgcatctcc tattttcggt ttgtatattc atgcaaataa
    69001 acgaagg
//
LOCUS       DAJTNM010000006        67477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTNM010000006 DAJTNM010000000
VERSION     DAJTNM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.006"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(21..779)
                     /gene="deoR"
                     /locus_tag="OO172_000490"
     CDS             complement(21..779)
                     /gene="deoR"
                     /locus_tag="OO172_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5510792.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWLIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(837..1433)
                     /gene="ybjG"
                     /locus_tag="OO172_000491"
     CDS             complement(837..1433)
                     /gene="ybjG"
                     /locus_tag="OO172_000491"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5510793.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1718..2944
                     /gene="mdfA"
                     /locus_tag="OO172_000492"
     CDS             1718..2944
                     /gene="mdfA"
                     /locus_tag="OO172_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5510794.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(2985..3270)
                     /locus_tag="OO172_000493"
                     /pseudo
     CDS             complement(2985..3270)
                     /locus_tag="OO172_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3356..4171)
                     /gene="ybjI"
                     /locus_tag="OO172_000494"
     CDS             complement(3356..4171)
                     /gene="ybjI"
                     /locus_tag="OO172_000494"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5510795.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4171..5379)
                     /locus_tag="OO172_000495"
     CDS             complement(4171..5379)
                     /locus_tag="OO172_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5510796.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5463..5999
                     /gene="rcdA"
                     /locus_tag="OO172_000496"
     CDS             5463..5999
                     /gene="rcdA"
                     /locus_tag="OO172_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5510797.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6174..7859)
                     /locus_tag="OO172_000497"
     CDS             complement(6174..7859)
                     /locus_tag="OO172_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5510798.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8129..8506
                     /locus_tag="OO172_000498"
     CDS             8129..8506
                     /locus_tag="OO172_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5510799.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8536..8793)
                     /gene="grxA"
                     /locus_tag="OO172_000499"
     CDS             complement(8536..8793)
                     /gene="grxA"
                     /locus_tag="OO172_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5510800.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            8953..9240
                     /locus_tag="OO172_000500"
     CDS             8953..9240
                     /locus_tag="OO172_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5510801.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9224..9947
                     /gene="nfsA"
                     /locus_tag="OO172_000501"
                     /pseudo
     CDS             9224..9947
                     /gene="nfsA"
                     /locus_tag="OO172_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10008..10910
                     /gene="rimK"
                     /locus_tag="OO172_000502"
     CDS             10008..10910
                     /gene="rimK"
                     /locus_tag="OO172_000502"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5510802.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            10998..11474
                     /locus_tag="OO172_000503"
     CDS             10998..11474
                     /locus_tag="OO172_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5510803.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11826..12938
                     /gene="potF"
                     /locus_tag="OO172_000504"
     CDS             11826..12938
                     /gene="potF"
                     /locus_tag="OO172_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5510804.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13033..14109
                     /gene="potG"
                     /locus_tag="OO172_000505"
     CDS             13033..14109
                     /gene="potG"
                     /locus_tag="OO172_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5510805.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14176..15129
                     /gene="potH"
                     /locus_tag="OO172_000506"
     CDS             14176..15129
                     /gene="potH"
                     /locus_tag="OO172_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5510806.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15126..15971
                     /gene="potI"
                     /locus_tag="OO172_000507"
     CDS             15126..15971
                     /gene="potI"
                     /locus_tag="OO172_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5510807.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16031..16519
                     /locus_tag="OO172_000508"
     CDS             16031..16519
                     /locus_tag="OO172_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5510808.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16560..17687
                     /gene="rlmC"
                     /locus_tag="OO172_000509"
     CDS             16560..17687
                     /gene="rlmC"
                     /locus_tag="OO172_000509"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5510809.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17862..18593)
                     /gene="artJ"
                     /locus_tag="OO172_000510"
     CDS             complement(17862..18593)
                     /gene="artJ"
                     /locus_tag="OO172_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5510810.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18885..19553)
                     /gene="artM"
                     /locus_tag="OO172_000511"
     CDS             complement(18885..19553)
                     /gene="artM"
                     /locus_tag="OO172_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5510811.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19553..20269)
                     /gene="artQ"
                     /locus_tag="OO172_000512"
     CDS             complement(19553..20269)
                     /gene="artQ"
                     /locus_tag="OO172_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5510812.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20276..21007)
                     /gene="artJ"
                     /locus_tag="OO172_000513"
     CDS             complement(20276..21007)
                     /gene="artJ"
                     /locus_tag="OO172_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5510813.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21025..21753)
                     /gene="artP"
                     /locus_tag="OO172_000514"
     CDS             complement(21025..21753)
                     /gene="artP"
                     /locus_tag="OO172_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5510814.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(21971..22486)
                     /locus_tag="OO172_000515"
     CDS             complement(21971..22486)
                     /locus_tag="OO172_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5510815.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23135..24904
                     /locus_tag="OO172_000516"
     CDS             23135..24904
                     /locus_tag="OO172_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5510816.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25115..25438
                     /locus_tag="OO172_000517"
     CDS             25115..25438
                     /locus_tag="OO172_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5510817.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25435..26265
                     /gene="amiD"
                     /locus_tag="OO172_000518"
     CDS             25435..26265
                     /gene="amiD"
                     /locus_tag="OO172_000518"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5510818.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26262..27275)
                     /locus_tag="OO172_000519"
     CDS             complement(26262..27275)
                     /locus_tag="OO172_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5510819.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVLTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27374..28804)
                     /locus_tag="OO172_000520"
     CDS             complement(27374..28804)
                     /locus_tag="OO172_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5510820.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANKHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28815..29816)
                     /gene="ltaE"
                     /locus_tag="OO172_000521"
     CDS             complement(28815..29816)
                     /gene="ltaE"
                     /locus_tag="OO172_000521"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5510821.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29853..31571)
                     /gene="poxB"
                     /locus_tag="OO172_000522"
     CDS             complement(29853..31571)
                     /gene="poxB"
                     /locus_tag="OO172_000522"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5510822.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31704..32672)
                     /gene="hcr"
                     /locus_tag="OO172_000523"
     CDS             complement(31704..32672)
                     /gene="hcr"
                     /locus_tag="OO172_000523"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5510823.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32684..34336)
                     /gene="hcp"
                     /locus_tag="OO172_000524"
                     /gene_synonym="priS"
     CDS             complement(32684..34336)
                     /gene="hcp"
                     /locus_tag="OO172_000524"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5510824.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34480..35379)
                     /gene="lysO"
                     /locus_tag="OO172_000525"
     CDS             complement(34480..35379)
                     /gene="lysO"
                     /locus_tag="OO172_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5510825.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(35836..36531)
                     /gene="aqpZ"
                     /locus_tag="OO172_000526"
     CDS             complement(35836..36531)
                     /gene="aqpZ"
                     /locus_tag="OO172_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5510826.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     VSGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            36956..38614
                     /locus_tag="OO172_000527"
     CDS             36956..38614
                     /locus_tag="OO172_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5510827.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38611..39567)
                     /locus_tag="OO172_000528"
     CDS             complement(38611..39567)
                     /locus_tag="OO172_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5510828.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39718..40833
                     /gene="macA"
                     /locus_tag="OO172_000529"
     CDS             39718..40833
                     /gene="macA"
                     /locus_tag="OO172_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5510829.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            40830..42776
                     /gene="macB"
                     /locus_tag="OO172_000530"
     CDS             40830..42776
                     /gene="macB"
                     /locus_tag="OO172_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5510830.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(42849..43073)
                     /gene="cspD"
                     /locus_tag="OO172_000531"
     CDS             complement(42849..43073)
                     /gene="cspD"
                     /locus_tag="OO172_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5510831.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43396..43716
                     /gene="clpS"
                     /locus_tag="OO172_000532"
     CDS             43396..43716
                     /gene="clpS"
                     /locus_tag="OO172_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5510832.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            43747..46023
                     /gene="clpA"
                     /locus_tag="OO172_000533"
     CDS             43747..46023
                     /gene="clpA"
                     /locus_tag="OO172_000533"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5510833.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46426..46513)
                     /locus_tag="OO172_000534"
     tRNA            complement(46426..46513)
                     /locus_tag="OO172_000534"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46477..46479),aa:Ser,seq:gga)
     gene            complement(46767..46985)
                     /gene="infA"
                     /locus_tag="OO172_000535"
     CDS             complement(46767..46985)
                     /gene="infA"
                     /locus_tag="OO172_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5510834.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47270..47974)
                     /gene="aat"
                     /locus_tag="OO172_000536"
     CDS             complement(47270..47974)
                     /gene="aat"
                     /locus_tag="OO172_000536"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5510835.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48016..49737)
                     /gene="cydC"
                     /locus_tag="OO172_000537"
     CDS             complement(48016..49737)
                     /gene="cydC"
                     /locus_tag="OO172_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5510836.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTQLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(49738..51504)
                     /gene="cydD"
                     /locus_tag="OO172_000538"
     CDS             complement(49738..51504)
                     /gene="cydD"
                     /locus_tag="OO172_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5510837.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51627..52592)
                     /gene="trxB"
                     /locus_tag="OO172_000539"
     CDS             complement(51627..52592)
                     /gene="trxB"
                     /locus_tag="OO172_000539"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5510838.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53137..53631
                     /gene="lrp"
                     /locus_tag="OO172_000540"
     CDS             53137..53631
                     /gene="lrp"
                     /locus_tag="OO172_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5510839.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            53766..57872
                     /gene="ftsK"
                     /locus_tag="OO172_000541"
     CDS             53766..57872
                     /gene="ftsK"
                     /locus_tag="OO172_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5510840.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            58027..58638
                     /gene="lolA"
                     /locus_tag="OO172_000542"
     CDS             58027..58638
                     /gene="lolA"
                     /locus_tag="OO172_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5510841.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58649..59992
                     /gene="rarA"
                     /locus_tag="OO172_000543"
                     /gene_synonym="mgsA"
     CDS             58649..59992
                     /gene="rarA"
                     /locus_tag="OO172_000543"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5510842.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60083..61375
                     /gene="serS"
                     /locus_tag="OO172_000544"
     CDS             60083..61375
                     /gene="serS"
                     /locus_tag="OO172_000544"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5510843.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61615..64059
                     /gene="dmsA"
                     /locus_tag="OO172_000545"
                     /pseudo
     CDS             61615..64059
                     /gene="dmsA"
                     /locus_tag="OO172_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64070..64687
                     /gene="dmsB"
                     /locus_tag="OO172_000546"
     CDS             64070..64687
                     /gene="dmsB"
                     /locus_tag="OO172_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5510844.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     HYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            64689..65552
                     /gene="dmsC"
                     /locus_tag="OO172_000547"
     CDS             64689..65552
                     /gene="dmsC"
                     /locus_tag="OO172_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR5510845.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(65588..66214)
                     /locus_tag="OO172_000548"
     CDS             complement(65588..66214)
                     /locus_tag="OO172_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5510846.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            66529..>67477
                     /locus_tag="OO172_000549"
     CDS             66529..>67477
                     /locus_tag="OO172_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5510847.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEH"
BASE COUNT        16773 a        16704 c        17691 g        16309 t
ORIGIN      
        1 cgtcatccgg ttatacgtca ttaatacatc aacttaatac ggtgcgtctg cgcgtacttc
       61 acatactcat cttccgggca acaatcactc accacaatat caaagcgttt caggtcaccc
      121 atgcgcgccg gacgcacctt gccaaattta ctgtggtcga caaccagcac atgcttttgc
      181 gccatcgaca tggcccagtg ttttaccggc aactcttcaa gattaaaaca ggtagcgcct
      241 ttactgacat gcacgcccgc cgcggaataa aaagcgatat ccgggcaaaa attattcaac
      301 gtttgctgaa aatcgatggg tttgaaaatg gcgttgctgg cgtgaaattc accaccgcac
      361 agaaatgcgc ggcaatgggg tttctctttc agcgccagaa aggtatttag cgaataacaa
      421 acggcggtaa aagggatttc attatcaatc gcttcaataa gccacggcgt ggtggtgcca
      481 cagtcaaaaa agagggtctg atcgggttct accagtgtcg cagccagttt tgccgcccgg
      541 cgtttttctt ccaccaggcg ggatttttga tcgcttaaca ggtaatggct ggcactgcgc
      601 ggttccagaa caatatagcc gccgagcaaa acgacgggcg cactgtggtt gttcagatcg
      661 cgacgaatcg tcatctccga aaccccaagc agggcggcgg cgtctttaag atgtaactta
      721 tcgctgcgtt ttaattcttg cagcagctgc ccgatacgct cttcgcgacg tgtttccata
      781 atccctctga atagttattg aagcgagccg ctcaatacta cactttttag cagagatcag
      841 tcacgcaccc agcctttgcg gatcggtaat gcaaaacaga cgcgatacca cgattgcaga
      901 cgttgataga gtttatgccc catcgcttgc cagataatct gggcactaag gcagccaatc
      961 atacctgcca gcaatccacc gagcatatcc agcggccagt ggacgccaag ataaacgcgc
     1021 gaccaggcaa tgacgacggc cagcaccatt aaaagtgagc cggaccacag gcgatgccag
     1081 cataaaaatg ccagtgcaaa ggtgaaaatc accgtaccgt gatcgctcgg gaatgagtca
     1141 tccgccgcat gatgcaggaa gttatagccg atattttcga caaagggtcg gtcgtgcgga
     1201 aaaagatgtc ccatcgtcca ggacacaaac aggctgaccg ccagcgcgat agcgattttt
     1261 atcaccagtt gccgttgtgc tgtaagcccc cacaaccaaa gtaccgcggc cagcaacggc
     1321 accacggtaa tcaaatcttt agcaataaaa atcgccaacg agatcatcca cggagccgag
     1381 tctggcgtcg cgttaataag agagaataga gagagattca aattttccag cataacttcc
     1441 cgacgcaaag tgattaaaag ggagccaata caggcaagtc gttgagaata aagtgcaggt
     1501 taaactgggt aaagcggcat cgtcttattt ccctcaagcg gcctgtttac ggtgggtgat
     1561 tgtaacgggc ataggttaaa taaaacttaa agaaagcgta gctatactcg caataatgta
     1621 agaatgtgct taaccgtggt ttcagctaca aaattcgctt tctcgttagc tgcgctttta
     1681 ttaaactctg cgcgattatt attggcgaag aaattgcatg caaaataaat tagcttccgg
     1741 tgccaggctt ggacgtcagg cgttactttt ccctctctgt ctggtgcttt acgaattttc
     1801 aacctatatc ggcaacgata tgatccaacc cggtatgttg gccgtggtgg aacaatatca
     1861 ggcgggcatt gattgggttc ctacttcgat gaccgcgtat ctggcgggcg ggatgttttt
     1921 acaatggctc ctggggccgc tgtcggatcg tattggtcgc cgtccggtga tgctggcggg
     1981 tgtggtgtgg tttatcgtca cctgtctggc aatattactg gcgcaaaaca ttgaacaatt
     2041 caccatgttg cgcttcttgc agggcataag cctctgtttc attggcgctg tgggatacgc
     2101 cgcaattcag gaatccttcg aagaggcggt ttgtatcaag atcaccgcgc tgatggtgaa
     2161 cgtggcgctg attgctccgc tacttggtcc gctggtgggc gcggcgtgga tccatgtgct
     2221 gccttgggag gggatgtttg ttttgtttgc cgcattggca gcgatctcct ttttcggtct
     2281 gcaacgagct atgcctgaaa ccgccacgcg tataggcgag aaactgtcac tgaaagaact
     2341 cggtcgtgac tataagctgg tgctgaagaa cggccgcttt gtggcggggg cgctgggatt
     2401 cgttagcctg ccattgctgg cgtggatcgc ccagtcgccg attatcatca ttaccgggga
     2461 gcagttgagc agctatgaat atggcttgct gcaagtgcct attttcgggg cgttaattgc
     2521 gggtaacttg ctgttagcgc gtctgacctc gcgccgcacc gtacgttcgc tgattattat
     2581 gggcggctgg ccgattatga ttggtctgtt ggtcgctgct gcggcaacgg ttatctcatc
     2641 gcacgcgtat ttatggatga ccgccgggtt aagtatttat gctttcggta ttggtctggc
     2701 gaatgcggga ctggtgcgat taaccctgtt tgccagcgat atgagtaaag gtacggtttc
     2761 tgcggcgatg ggaatgctgc aaatgctgat ctttaccgtc ggtattgaaa tcagcaaaca
     2821 tgcctggctg aacgggggca acggactgtt taatctcttc aaccttgtca acggaatttt
     2881 gtggctgtcg ctgatggtta tctttttaaa agataaacag atgggaaatt ctcacgaagg
     2941 gtaaaaaaat gcctgattga tttgtgcgat caggcattct cgaattaatg gtgatggtcg
     3001 tcaatctggt gttcgataac catcccttca cctacgctgg caagatggcg aacataagga
     3061 tgcgggcggt aagccggagc tggcacagga gccacataga cggtttgcgg cacagtcgcg
     3121 acgctgacac gcttgtggaa cgctgactga atcaggaacc acaaccactt tgtatccact
     3181 cggcacatcg acggtgacac tttgcgccat cgccacacca gtaaagccca ttaaaagcgc
     3241 gcccaacagt agacaatttt tcataatcat ttcgcctgaa ataatgcagc gggttaattt
     3301 ccgcgaatta tgcagccttc attcaggcat tgtgttgccg ggcgtgctgc cgtttttatt
     3361 ggtcaaatgg cgcttcgtgt ttaagaactt tatcgatcac atccagtacg ccttcacggt
     3421 tattggagcc tgcccggtat tttgccgctg cgacgaccgc gctgccggca tttttcattg
     3481 caaaactaaa gcctgcctga cgcagcatct caatatcgtt accgccatcg ccaaagacca
     3541 ccacttcgct gtcgtctatt ccccataatt tctgcagttg gcgaaggcca ttggctttat
     3601 gtacgccggg gataatcaga tcgatgctgc cgttgccggt gtggactggc accataatat
     3661 cgccgatggc ctcatgtaat gctttttgta cttgtggaat cagttcatcg gaaagattca
     3721 ggccaaactt aaagaaaata tcttctaagt tgtcaaagtt atcgacgtat tccagacggt
     3781 gataatacat ttccgccacc gttttcatgg catcgtcata ttttttgagt gtataggcgc
     3841 tattttttcc gcaggcaata atttccactt ccgggcgcgt cagcaaatgt tccacgacag
     3901 tagcaaacgc atccttcgac agctcgccat taaaaacatc tttgccttca ctcactaccc
     3961 agccgccgtt ttcagccaca aaggcaattt cattcgcaat ttcagggaag aaagagatca
     4021 actgataata ttgattcccg ctggcgacca caaagcgaat gccttgtgct ttcatttgct
     4081 gatactgagc cataaaccgc tcacggttat aggttttttg atcgcttaag aaagtaccat
     4141 ccatgtctac cgcaattaat ttaatactca tcaactattc tccatcgccg tctgcgtttt
     4201 ggtatcgggt ttggcgacgg ctttcgcgac aatagccgcg agaataacca gcgccagtac
     4261 aaccagcatt gcactacgta atccataatg ttcgccgaga tagcccagca gcggcggccc
     4321 gacgaggaaa gccagataac cggtcgttgc taccacactg acgcgggttg gtgcatcggg
     4381 gccggtatcg ctggcggcag aaatggtcag cgggaagccc agcgaggcac ccagtcccca
     4441 aagtacaaca gacactcctg cgacccaggc gctatcgaca aaaataatca gcccaatacc
     4501 caacgccccc attagtgcac tggcccgaac cacggcaacg cgactgtaac ggtcgatgaa
     4561 ccaaccgccg gtgaagcgtc caacggtcat ccccagggta aaaccggcat aaatcagtga
     4621 gccggaagtg gggctaaaac cgtgaccatc aaccattaat aagggtaacc agtcgttggc
     4681 agaaccttcg gcaaaggcca tcgccagcac tacaacacca atcaacagca actggatatc
     4741 gcgataaaaa ggaatgcctt tttcgccatg ctgggtgcca tcggcagcat ttttgcccgt
     4801 accgtcaggg attgcctgaa tggcgatata aataggcgcg atacctacca gcgccgccaa
     4861 taaaatgtgc accgttgccg gaacgccaaa ggccgtcagt gccatcccga caccagcgcc
     4921 tgccagcgtg cccaggctat aaaaaccgtg catcatcggc aaaaccgttt tattcatttc
     4981 tcgctcaacg gcggcacctt caacgtttat cgccacttcc gcagaaccaa aacttgcccc
     5041 aaagacgccg agaccaacgg caaagagcag gggcgatgtc agccagagtg ccagacttaa
     5101 tatcatcatc ccgatcaatg cgcaggacat cgtgaccagg atgacattac gtgtcccaaa
     5161 gcgtttcact aaccacgccg agcagagaat accgctcatc gaaccgatcg acagaccaaa
     5221 gagaacaccg cccatttcag cgatcgagac agagagaata tcgcggatag caggcgtacg
     5281 ggttgcccag gacgccatta acaggcctgg caaaaagaag aacataaaca gcgccctggt
     5341 tcggcgtttc aatgcattac gtgaagaatt tacggtcata gatcacgtca aaataagaag
     5401 agggaagaca acattagcaa ggttgtgtac atttgtacac aattgcagac agaggaaatg
     5461 acatgcgtcg cgctaacgat ccgcaacggc gagaaaaaat tatccaggcc acgctggagg
     5521 cggtgaaact ttacggaata catgctgtta cgcaccgcaa aattgctacc cttgccgggg
     5581 taccgttggg atcgatgacc tactattttt caggaattga tgagttgttg ctggaagcgt
     5641 tcagcagttt tactgagatt atgtcccggc aatatcaggc attttttagc gatgttagtg
     5701 atgctcaagg cgcatgccag gctatcaccg atatgatcta cagctcacag gttgcaacgc
     5761 cggataatat ggagctgatg taccagctct acgcgctggc tagccgaaaa ccgctattaa
     5821 aaacggtaat gcaaaactgg atgcagcgca gtcagcaaac gctcgaacaa tggtttgaac
     5881 ccggaaccgc ccgcgcgctt gatgcgttta ttgaggggat gacgctgcat tttgtcaccg
     5941 accgtaagcc gctatcgcgc gaggagattt tgaggatggt tgagagggtt gcagggtagt
     6001 agataaattt tagataacaa aaaacccatc aaccttgaac cgaaatggcg gggttgatgg
     6061 gctccacaaa atggggacat caaagaaaag cagtggcact aattaagact gatgccctgc
     6121 ggaaaagttc tgcggttgtg caaaaaaatt tcattttcag ggcaacttca gttttatcct
     6181 aatcctggcc ataccatgac gatgattgtc cctgccagcg tcagcaggac gttggtgatt
     6241 gcgtaggtgc ccgcatagcc cagcgcaggg atgttactgc gagctgtatc actgatgatc
     6301 tccattgccg gcgcgcaggt gcgtgcgccc atcattgcgc cgaacaacag tgcgcggttc
     6361 attcgcaata cataagcacc gaacaagaaa cagataacca cgggcacaag actgacaatt
     6421 aatccggcaa tcaacatctg accgccaatc gcgcccaggc cgttattaat accgctaccg
     6481 gcgctcagac caacgcctgc cataaacacc atcaagccga actctttcac catgcttaat
     6541 gcaccttgcg gaatgtaacc gaaggtcggg tggttagcac gcataaagcc cagcataatt
     6601 ccggcgaata acaacccggc agcgttcccc atgccgaaac tgaatgtgct gaactggaag
     6661 gtgatcatcc cgatcatcag cccaataaca aagaaggcgc aaaatgccag caggtcagtg
     6721 acctggctgt gaatcgagat aaagccgatg cgatcggcga tggtttttac gcgacgggca
     6781 tcaccgctga cttgtaaaac gtcacctttg ttaagcacga cgttgtcatc tatcggcatc
     6841 tcaatctggc tacgaatgac gcggttaagg aagcaaccgt gatcggtcaa cttcagttgt
     6901 gcgagacgtt tacctacagc gttatggttt ttaacgacca cttcttcagt gacgatacgc
     6961 atgtcgagaa ggtcacgatc gaaaacttct ttaccgttac ggaagctggg atcgagtcgg
     7021 gcatgggcgt cgggatagcc taccaacgct atttcatcgc ccatttgtag cacggcatca
     7081 ccgtctggat ttgccagaat cccgttacgt cgaatacgtt caatgtagca gccggtttgt
     7141 cgataaatac ccagttcacg cagatttttg ccgtcggtcc aggccaccag ctccgggccg
     7201 acgcgatagg cgcggatcac cggtaaataa accttacggt tggcatcagt gtccaggcca
     7261 cgttcgcggg cgatttgctg ggcgctggtc tgtaagtcct gatgctgcaa tttcggcaag
     7321 taacgcgcac caacaatcaa actcaccaga ccgattaaat aggttaaggc atacccgagg
     7381 ctcagattat ccagtgccag tgagagctgc ctgctttcca tgccggaatg atgcagtgta
     7441 tcgccagcac cgaccagaac cggtgtcgac gtcatagagc ctgctaacat accggccgtc
     7501 aggccaatat cccagccaaa cagcttacct aaccctaagg cgatcaccag cgcactgcca
     7561 accatcacca gtgctaacat taggtaattt ttcccatcgc gaaaaaaaat ggaaaaaaag
     7621 ttcggtccgg cttcgacccc gacgcagaaa ataaacagca taaagccaag attaagcgca
     7681 tcggtgttaa tgctgaaatg ttgttggcct aataacagcg atacgactaa aacgccaatg
     7741 gaattaccca gttggatcga accaagtcgt aactttccga gacatagccc aagcgcgagg
     7801 accacaaata ataacagaat gtaattccca tttaacaatt cggcgacgtt tatattcacg
     7861 gaggctaact tcttgtttac tagtaagctg ttgaaagaaa tggtaattta cgataatgtg
     7921 ttttaccaga attcagggtg cagattcatt cagcgcacct aaacgatagt aaagtaacaa
     7981 tatattttac tagtgtaatc acattaggta tcaacggcta tatgaattgc gttggcctat
     8041 attagcatgg aatgcgaagc ggctttatct tactgaacgc cacactggcg aaaaatgtgt
     8101 tcgatagacg cagtgtcagg aggaacgagt gaaacataaa caacgttggg cgggggcaat
     8161 ctgctgtttt gtcctcttca ttgtggtgtg cctttttctg gcgacgcaca tgaaaggcgc
     8221 ttttcgggct gccgggcatc ctgaaatcgg cttgctgttt ttcattcttc ctggagcagt
     8281 tgccagtttt ttttcacagc gtagagaagt cctgaaacct ctatttggcg caatgctggc
     8341 ggcaccctgt tcaatgctca ttatgcggct gtttttttca ccgacgcgct cattctggca
     8401 agagctggca tggttactaa gcgcggtgtt ctggtgtgcg ctgggggcac tgtgtttctt
     8461 atttatcagt agtttgttta aaccacagca cagaaaaaat cagtaaagcc ctcaacgcga
     8521 gggcttgtca gacgatcagg cgtccagatt ttctttcacc catgcagcaa aatcggtata
     8581 gccgccgata tgttgctgat cgacaaaaat ctgcggcacg gtttctacgg gtttacctgc
     8641 cttttgttgt agatcttctt tagtgatccc ttccgcacga atatctacat actgatactg
     8701 aaaatcatcg cgttcattgc tcaatttctc agccagatct tttgcacgca cacagtaagg
     8761 gcaacccgaa cgaccaaaaa taacggtttg cattatttct ctcctcatag atttatgcct
     8821 gtaatgatca cgctaaaatg tattcgctga aagtaggttt aacctgttgc attaattgct
     8881 aaaagctata actgttaaac acaatacagt gaaaagtttt agactgaagg ctcactttgc
     8941 agagggaagc gtatgcgcgc gatcggtaaa ttgcctaaag gcgtgttgat actggaattt
     9001 atcggaatga tgctactggc ggtggcgctg ctgtcggtaa gcgactccct gtcgctgcct
     9061 gagccatttt ctcggccaga agtgcagatt ctgatgattt ttctcggtgt tttgctcatg
     9121 cttcccgctg cggtggtggt tattcttcag gtggtaaaac gtcttgcccc acagctgatg
     9181 aaccgtccac cgcaatattc acgttcagaa agagaaaaag ataatgacgc caaccattga
     9241 acttatttgt ggccatcgct ccattcgcca tttcactgat gaacccattt ccgaagcgca
     9301 gtgtgaggcg attattaaca gcgcccgtgc gacgtccagt tccagttttt tgcagtgtag
     9361 tagcattatt cgcattaccg acaaagcatt acgtgaagaa ctggtgacgc tgaccggcgg
     9421 gcaaaaacac gtagcgcaag cggcggagtt ctgggtgttc tgtgccgact ttaaccgcca
     9481 tttacagatc tgtccggatg ctcagctcgg cctggcggaa caactgttgc tcggtgtcgt
     9541 tgatacggca atgatggcgc agaatgcatt aaccgcagcg gaatcggctg ggattgggcg
     9601 gggtatatat cggcggcctg cgcaataata ttgaagcggt gacgaaactg ctgaaattac
     9661 cgcagcatgt tctgccgctg tttgggctgt gccttggctg gcctgcggat aatccggatc
     9721 tcaagccgcg tttaccggcc tccattttgg tgcatgaaaa cagctatcaa ccgctggata
     9781 aagacgcact ggcgcagtat gacgagcaac tggcggaata ttacctcacc cgtggcagca
     9841 ataatcgctg ggatacctgg agcgatcata tccgccgaac aatcattaaa gaaagccgcc
     9901 catttattct ggattatttg cacaaacagg gttgggcgac gcgctaaaac cgccacgtcg
     9961 atgtatgata cgcgggcttt tgaccaggtc tgacagagag gtgcagggtg aaaattgcca
    10021 tattgtcccg ggatggaacg ctctattcgt gtaagcggct gcgtgaagcc gctatacagc
    10081 gcggtcacct ggttgaaatt cttgatccgc tttcttgcta catgaacata aatcctgcgg
    10141 cgtcttctat tcactacaaa ggccgcaagt taccccattt tgacgcagtg atcccgcgta
    10201 ttggcaccgc cattaccttt tatgggacgg cggcactgcg ccagttcgag atgctgggga
    10261 gctatccgct caatgagtcg gtcgccattg cccgggcgcg tgacaaattg cgttccatgc
    10321 aactgctggc gcgtcagggc atcgacctgc ctgtcacggg cattgcgcat tcgccggatg
    10381 ataccagcga tttaatcgac atggtcggtg gtgcgccgct ggtggtcaag ttggttgaag
    10441 gcacgcaggg aattggcgtc gtgctggcgg agactcgtca ggcggcggaa agcgtgattg
    10501 acgctttccg cggtctgaac gcgcatattc tggtgcagga atatatcaaa gaggcgcaag
    10561 ggtgcgatat ccgctgtctg gttgttggtg atgaagtggt cgctgcgatt gaacggcggg
    10621 cgaaagaggg ctattttcgt tccaatttgc atcgtggcgg cgcggcaagc gtcgccagta
    10681 tcacaccaca ggagcgtgaa atcgcgataa aagccgcgcg aacgatggcg ctggacgttg
    10741 ctggtgtgga tattctgcgt gctaatcgcg ggccgttggt gatggaagtg aatgcgtcgc
    10801 cggggctgga aggaatagaa aaaaccaccg gtatcgatat cgcgggtaaa atgatccgct
    10861 ggatcgaacg ccacgctacg acagaatatt gcctgaaaac gggtggttag tcgcaatcac
    10921 attactgatc atggttttgc ctgcgctttt tgcgtaagct gtgccggtct ttttatcgaa
    10981 agaggttgta caaaattatg acatcgctgg tcgttcctgg tctggatacg ctgcgtcaat
    11041 ggctcgatgg cctggggatg agtttttttg aatgtaataa ctgtcaggct ctgcatctgc
    11101 cccatatgca gaatttcgac ggtgtctttg atgccaaaat cgatctgatc gataacacga
    11161 tcctgttttc tgccatggcg gaagtccgac cttcagccgt attgccgctg gcggcggatt
    11221 tatctgccat caatgccagt tcgctgaccg tgaaagcatt tcttgatatg caggatgata
    11281 atctgccaaa gctggtggtt tgccagtctt tatccgttat gcagggcgta acctatgagc
    11341 agtttgcatg gttcgtgcgt cagagcgaag agcagatttc gatggtcatt cttgaagcta
    11401 atgcccatca actgctgtta ccgactgacg atgaagggca aaacaacgtt accgaaaact
    11461 attttctcca ctgataattc ctttcgagca cgcagtcgct gccgcagtga ctgcgcgctg
    11521 caaaattatc tgctgttttt aaccttttct taaagattat ttcacttctc ttgtgtcgat
    11581 ttggctttat cacatagagc aaatacgcat aaaaatttgt taaataccgt tttttaatcc
    11641 gagctatagt ctcaaaccct ggctaaagtt attcttgcga tgcttctata tagcgagcag
    11701 tgctggccgg gagagagttc tcttttctta caccgcgccg ataaaaaaat atgcacgttt
    11761 attgcatatc tttcagtgtg acaacttttg ttcgtttgtt aacgaacttt cagaaggaaa
    11821 gagatgtgac cgccttaaat aaaaaatggc tatcgggtct ggttgcgggt gctctgatgg
    11881 ccgtctctgt cggcacgctc gcggctgaac aaaaaacact ccacatttat aactggtctg
    11941 attatatcgc ccctgacacg gtggccaatt ttgaaaaaga aaccggtatt aaagtcgtct
    12001 acgatgtttt cgactctaac gaagtactgg aaggcaaatt aatggcgggt agcaccggct
    12061 ttgacttggt ggttccatct gccagctttc ttgaacgcca gttgactgcg ggagttttcc
    12121 agccgctgga caaaagcaaa ttgccggagt ggaagaatct cgacccggag ctgctgaagc
    12181 tggtcgccaa acacgatccc gacaataaat ttgctatgcc ctatatgtgg gcgacgaccg
    12241 ggattggcta taacgttgat aaagttaaag ctgtgctggg cgaaaacgcg cccgtcgata
    12301 gctgggactt gatcctcaaa cctgaaaatc tggaaaaact gaaaagctgc ggtgtctctt
    12361 tcctggatgc gccagaagaa gtttttgcta ccgtgttgaa ttatctcggc aaagatccca
    12421 acagcactaa agcggatgat tacaccggac cggcaaccga tctgctgtta aagctgcgcc
    12481 cgaacattcg ttatttccat tcatctcaat acattaacga cctggcaaac ggcgatattt
    12541 gcgtcgctat cggctgggca ggtgatgtct ggcaggcgtc aaaccgcgcg aaggaagcga
    12601 agaatggagt gaatgtctcg ttctcgattc caaaagaagg ggcgatggcg ttctttgatg
    12661 tattcgccat gcctgcggat gccaaaaaca aagacgaagc ctatcagttc ctgaattacc
    12721 tgctgcgccc ggatgtagta gcgcatattt ccgaccatgt gttctatgtc aacgccaata
    12781 aagcagccac gccgctggtg agtgcggaag tccgtgagaa cccgggtatt tatccgcctg
    12841 cggatgttcg tgcgaagctg ttcactctga aagtgcagga cccgaaaatc gaccgtgtgc
    12901 gcacccgcgc gtggaccaaa gtgaagagcg gaaaataatc cgcagcctta gatgccgggc
    12961 gggcgcacca cacccgccgg caattcgcac catcatggtg cgcttgcaca cattcaatgc
    13021 cggagagcag ccgtgaatga cgctatccct cgcccgcagg cgaaaacccg taaggtgctg
    13081 acgccgctat tagaaatccg caacctcacc aaatcctacg atggtcaaca tgcggtggat
    13141 gatgtcagcc tgaccatcta caaaggtgaa atcttcgcgc tgctgggcgc atccggctgt
    13201 ggcaagtcca cgctgctgcg tatgctggca ggtttcgaac aaccttctgc cggacagata
    13261 atgcttgatg gcgtcgattt gtcacaggtt ccgccttacc tgcgccccat caatatgatg
    13321 tttcagtctt acgcgctgtt tccgcatatg accgtggaac agaacatcgc ttttggcctg
    13381 aaacaggaca aactaccgaa agcggaaatt tccagtcggg tcaatgagat gctcggcctg
    13441 gtgcacatgc aggagttcgc caaacgtaaa ccgcatcagc tttctggtgg tcagcgacag
    13501 cgtgtggccc tggcccgaag ccttgccaag cgcccgaaac tattactgct cgacgagccg
    13561 atgggcgcgc tggataaaaa gctgcgtgac cggatgcagc ttgaagtggt ggatattctg
    13621 gagcgcgtcg gtgtgacttg tgtgatggtc acccacgatc aggaagaggc gatgaccatg
    13681 gcggggcgca tcgccattat gaatcgtggg aaatttgtcc agattggcga accggaagag
    13741 atctacgagc atccgactac ccgctatagc gccgaattta ttggctcggt aaacgtcttt
    13801 gaaggcgtac tcaaagagcg tcaggaagat ggcctggtgc ttgattcgcc ggggctggtg
    13861 catccactga aagtcgacgc ggatgcctcg gtggtcgata acgtgccggt acatgtggcg
    13921 ctgcgcccgg aaaaaatcat gctttgcgaa gagccgcccg ccaatggttg taacttcgcg
    13981 gtgggggagg tgatacacat tgcctatctc ggcgatcttt cggtgtatca cgttcgtctg
    14041 aaaagtgggc agatgattag cgcccagcta caaaacgccc atcgtcatcg taaagggtta
    14101 ccgacctagg gcgacgaagt gcgtttgtgc tgggaagtgg acagcagtgt ggtgctgacg
    14161 gtttaaggag caaagatgaa tacacttgaa cctgctgccc agtcgaaacc gccgggcgga
    14221 tttaagctgt ggttgtcaca gctgcaaatg aagcatgggc gcaaactggt cattgcgttg
    14281 ccatatatct ggttgatctt gctgtttctg ctgccatttc tgattgtctt taaaataagc
    14341 ctggcggaga tggcgcgcgc tattccacct tataccgaac tgatggagtg ggctgacggg
    14401 caactgtcca tcactcttaa tctcggtaat tttctgcaac tgaccgacga tccgctctat
    14461 ttcgatgctt atctccagtc gctacaggtg gctgcgattt cgacattttg ctgtttactg
    14521 atcggctatc cgctggcgtg ggcggtggcg cacagtaagc cttcgacccg taatatttta
    14581 ttactactgg tgatcctgcc gtcgtggacc tcgtttctga tccgcgttta tgcctggatg
    14641 ggaatattaa aaaacaacgg tgtgctgaat aattttctgc tgtggctggg ggttatcgat
    14701 caaccgctga ccattcttca taccaatctg gcagtttata ttggcattgt ttacgcctac
    14761 gtgccgttta tggtgctgcc gatttatacc gcgttgattc gtattgatta ttcgctggtg
    14821 gaagcagcgc tggatctcgg tgcacgaccg ctgaaaacgt tctttactgt gatcgtgccg
    14881 ctgactaaag gtgggattat tgccggatcg atgctggtgt ttatcccggc tgtgggcgag
    14941 tttgtgatcc cggaactgct cggtggcccg gacagcatca tgatcgggcg cgtgctatgg
    15001 caagagtttt tcaataaccg cgactggccg gtggcctcgg cggtagcgat catcatgttg
    15061 ctgctgctaa ttgtgccgat aatgtggttc cacaaacacc agcaaaaaag cgtgggagaa
    15121 cacggatgaa taatttaccg gtagttcgtt cgccctggcg gattgtgatt ttgctgctgg
    15181 gctttacctt tctctacgcg ccaatgctga tgctggttat ctattcgttt aacagctcga
    15241 agctggtgac ggtgtgggcc ggctggtcaa cgcgctggta tggtgagtta ttgcgcgatg
    15301 acgcgatgat gagtgcggtt ggttcaagcc tgacaattgc tgcctgtgcg gcaacggcgg
    15361 cggcgatcct cgggactatt gcggcggtgg tgctggtgcg ctttggcagg tttcgcggat
    15421 caaatggctt tgcctttatg atcaccgcgc cgctggtaat gccagatgtc atcacgggct
    15481 tgtcgctgtt gttattattc gtcgcgcttg ctcatgccat tggctggcct gcggaccgcg
    15541 gtatgcttac catctggctg gcgcatgtca ctttctgtac ggcttatgtg gcggtcgtta
    15601 tttcgtcgcg tctgcgggaa ctggatcgct cgatagaaga agccgcgatg gatctcggtg
    15661 cgacgccgct gaaagtgttt ttcgtcatta cgctaccgat gatcatgccc gcgatcattt
    15721 ctggctggtt actggctttt actttgtcgc ttgatgatct ggtgatcgcc agctttgttt
    15781 ccgggccggg agccaccacg ttaccgatgc tggtcttttc cagcgtgcgg atgggggtga
    15841 atccggaaat caacgccctg gcaacattga tcctcggcgc ggtcggaatt gtcggattta
    15901 tcgcctggta tctgatggcg cgcgcagaaa aacagcggat acgcgacatc caacgtgcaa
    15961 gacgtggctg aagacactaa aatttgccaa cctggctaca taatgctgcg catgtcgcgg
    16021 cattgttttc atggaagacg aaacgttggg attttttaag aaaacatctt catctcatgc
    16081 tcgcctgaat gtgcctgcgc tggtgcaggt ggcggcgctc gccattatta tgatccgtgg
    16141 cctcgacgtg ctgatgattt tcaatacgct gggcgtgcgc ggtattggcg agttcattca
    16201 tcgcagcgta caaacctgga gtttaacgct ggtcttttta agcagtctgg tgctggtttt
    16261 cattgagatc tggtgtgcgt tttcactggt gaaagggcgt cgctgggcgc gctggctata
    16321 tctgctgaca caaatcaccg ccgcaagtta cttgtgggcg gcttcgctgg ggtacggtta
    16381 tccggagctg ttcagcattc ccggtgaatc aaaacgtgaa atcttccata gcctgatgct
    16441 gcagaagctg ccggatatgc tcatcctgat gctgctgttc gttccctcga ccagtcggcg
    16501 gttcttccag ttgcaataat gtgtataatc gtcgcccctg atgatgtgaa ggtcaatgta
    16561 tgcagtgcgc actttacgac gcgggtcgct gtcgttcctg tcagtggata acgcagccga
    16621 ttccagagca actctccgct aaaaccgccg atcttaaaaa tctgctcgcc gattttccgg
    16681 ttgaggaatg gtgcgcgccg gtgtcaggcc cggaacaagg gtttcgtaat aaagccaaaa
    16741 tggtggtgag tggtagcgtt gaaaaaacac tgctcggtat gctgcatcgc gatggcacgc
    16801 cggaagacct ttgtgactgc ccgctttatc cagcctcatt tgcgcccgtt tttgcggcgc
    16861 taaaaccgtt tatcgcccga gcggggttaa cgccctacaa cgtggcgcgt aagcgtggcg
    16921 aactgaaata cattctgctg actgaaagcc agagtgatgg cggcatgatg ctgcgctttg
    16981 tactgcgttc tgataccaag ctggcgcaac tgcgtaaggc gctgccgtgg ttacacgaac
    17041 aactaccgca gctgaaagtt attaccgtca atattcagcc ggtacatatg gcgattatgg
    17101 aaggggagac ggagatctac ctgaccgaac aacaggcact ggcggagcgt tttaatgacg
    17161 taccgctgtg gatccgtccg caaagtttct tccagactaa tccggcggtc gccagccagt
    17221 tgtacgccac cgcgcgcgac tgggtacgac agctgccggt taaacatatg tgggatctgt
    17281 tctgcggtgt ggggggcttt ggtttacact gcgcgacgcc tgacatgcag ttaaccggga
    17341 tcgaaattgc gccagaggcc attgcctgtg caaagcagtc agccgctgaa ctgggcttaa
    17401 ctcgtttaca atttcaggcg ctggactcca ctcagtttgc cactgctcag ggggaggtgc
    17461 cggagctggt gctggttaac ccgccgcgcc gcggcattgg taaaccgctg tgtgattatc
    17521 tttcaacgat ggcaccgcgt tttattatct actccagctg taacgcccaa accatggcga
    17581 aagatatccg cgaactgcca ggttaccgca tcgaacgggt acagcttttt gatatgttcc
    17641 cgcacaccgc gcactatgaa gtgttgacgc tgctggtgaa gcaataaaaa agccacaggt
    17701 gcggcttcag attgctgatc aggtgcgctt tgttcatgcc ggatgcggcg tgaacgcctt
    17761 atccggccta caaaatcatg ctaaatcaat acattgcaga gttgatgtgg gccggataag
    17821 cgcagcgcat caggcaatct tacttttgtc atcagtctca attactgcgg aaaccactgg
    17881 tcattgattt tctgatatgt accgtcagct ttaattgctg ccagcgcgtt attcagtttt
    17941 tccagcagag ctttgttatc cggacgtaca gcgataccca ggccggtgcc gaagtattgc
    18001 ggatcggtca ctttttcagt cgcaacgccc agttgcggat tggttttcag ccattcgttt
    18061 accaccgcgg tgtcaccaaa taccccatca atacgaccat ttttcagatc gataaaggca
    18121 ttctgataac tgtcatagga gacagttttc acttccgggt gctgatcctg aatatatttc
    18181 tggtgcgtcg taccgttttc catcccaatg cgtttgcctt tcaggtcggc aaacgttttg
    18241 taggtatctt ttttggcaat cacgacggct gagttttcat agtagggtgt ggtaaacgaa
    18301 acctgcttgc tacgctccgg ggtgatatcc ataccggaga ttacggcgtc atattttctg
    18361 aatttcagtg acgggatcag gctgtcgaac gcgtgattag taaaagtaca ttctgcctgc
    18421 atttgtttgc acaaggcttt tgccagatcg atatcaaagc cgacaatctc attattagca
    18481 cctatagatt caaagggtgg ataggtggct gaaacgccaa aattgatttt ctctgcggca
    18541 gaagcaccga aagtaaagga agcaagtaaa gcggcaagaa ctaacttttt catgatggaa
    18601 ctcccgtctg tcaatcttat gatttttggc cgtgtctgcg gcatgggata acaatgccac
    18661 caagtgaatt tatatgcaat aaacatgatt aaataattta aatgaaataa aaaaagacgg
    18721 ataacttagt tagttgtccg tcttcattat aagaatttat gcactatgta ggccggataa
    18781 ggcgtccacg ccgcatccgg cacaggcacc gtgctgatgt ctgatgcgac gctggcgcgt
    18841 cttatcagac ctacaaatcc ccccggcgaa tgtacgcagc tacattaatt ccgccgttcg
    18901 aatgccagtg ctttgcgctc gatcagacgc atcatcagcg tcagcaggcc gttgacgacc
    18961 aggtaaataa tccctgccgc accgaacacc attacatcgt aggtgcgtcc gtacaacaac
    19021 tggctgtatc ccatcacttc catcagcgta atggtgtaag ccagagaggt acttttgaac
    19081 accagcacca cttcgttgga ataagaagag agcgagcgtt taaaggcata cggcagcagg
    19141 atcgccagcg tatctttttt gctcattccc agggcgctac aggactgcca ctgaccttct
    19201 gggatcgcac gaattgcacc gtaaaacagc tgcgtggtat acgccgcact gttcagcgat
    19261 aacgcaatca gcgcacataa ccacggttct gacaacaaat gccacagtgc cggatactcc
    19321 tgcaacgtcg gaaactggcc cggcccgtaa taaatcagga agatctgcac cagcagcggc
    19381 gtaccggtaa acagcgtgat ataaccccgc accagccaca ccagcaccgg cgttttcagc
    19441 gtcaggatga tggtaaaaat caatgccaga atcagtgcca caatcagcga ggcaacggtt
    19501 agcgtcaggc tggtgtgcag ccctttcatc agctcgggta aatactcaaa cattagctgg
    19561 gcctccgctc aaaacgtgtc gcgcgcaggt caatgcgttt gagaatgtac tgactgagca
    19621 gggtgatcac caggtaaatc gccgccgcca caatgtacca ggtaaatggt tcctgggtac
    19681 gagtagcaat gcttttggtt tgtaacatta aatcattcac actaatcaaa ctgaccagcg
    19741 cggtatcttt cagcagcacc agccactggt taccgaggcc aggcagcgca tgacgccaca
    19801 tctgcggcat caccagacgg aaaaagatag ccgatttcga cagccccagc gcctgaccag
    19861 attcccactg gcccaccggt acagctttca acgcgccgcg cagcgtttgc gaggcatagg
    19921 cggcatacag cagtgacaga gcgatgacac cacaaaggaa cggactcacg tcgaagttct
    19981 caatgtccat ctgcaccggg atctgcacga acccaagatt gatagtgaag ccatccgaaa
    20041 gcgtcagcag taactgcgag gagccaaaat agataaacag cactaccaga atttctggca
    20101 ggccacgcag aatggttacc agcgctgaac ctgcccacgc gacaggacgc catttcgctg
    20161 actcccatac cgcaaagaac atcgccagcg ccagcccgac aatcaatgca caaacggcaa
    20221 ggccgacggt catcccggcg gcgcttgcta aaggaaaaaa ttcattcatc aggaattact
    20281 tctggaacca tttgttgtag atggtttcgt aagtgccatc tttcttcact ttttccagcg
    20341 cagtgttgag tttctgctgc agttcagtgt tgccctgacg taccgcgatg ccgaggccag
    20401 taccgaaata atctttatcg gtcactttat cgcccactgc cgccagtttt gggttatctt
    20461 tcagccactc agtgaccact gcggtgtcac caaatacgct gtcgatacgc ccgttttgca
    20521 gatccagttt tgcgttctgg tagctgtcat acggaacggt ggtgatttcc gggtgcttat
    20581 ccataatgaa cttctggtgt gtcgtcccgt tctgaacgcc gacttttttg cctttcagct
    20641 gatcaacact ggtgtatttg ccttgctgac ccacaaacag ggcagagttg tcatagtacg
    20701 gggtagtaaa cagtacctgc ttttcacgct ccggagtgat atccatgccc gccatcactg
    20761 cttctacgcg acggaatttc aggcttggga tcaggctgtc aaacgcctgg ttagagaaag
    20821 tacaggttgc atcaatctct ttacacagtg cctgtgccag gtcgacgtca aaaccaacga
    20881 tctggttgtt tgcatcaatc gattcaaacg gaggatagga ggcttcggta gcaaaacgaa
    20941 tggtttcggc agctgtggcg gaaagactaa aacctgcaat taacgcggca atcagaactt
    21001 ttttcattgt tgttatcccg aatcttagtg agagagatag tttttaaatg cttcggtttg
    21061 cggctcggta aagcagctcg cgtcgccttg ttctacgata tgaccatttt ccatatacac
    21121 cactcggctg gcggttttac gcgccacttc aacttcgtgg gtgacgatca cctgggtaat
    21181 attcgtttct gccagctcac gaatgatgct gacgatttgt gccgtaattt ccgggtccag
    21241 tgcggcggtc ggttcatcga acagcagtac ctgcggttcc atcatcaacg cacgggcaat
    21301 agcaacacgc tgctgctgac caccagaaag atgcagcggg taacgatcgc tataaggttt
    21361 gagacgcaga cgttccagca gtttttctgc acgggccagc gcctgatctt tactcaaccc
    21421 cagtacacgg cagggcgctt caatcaggtt ttgctgcacg gtcagatgcg gccacaggtt
    21481 gtattgctga aacaccatgc caacgttacg acgcaaatcg cgaatcgctt tgtcagaggg
    21541 tgttttggtg aaatcgaaat ggttgcctgc aatgttgagc gtaccggagc gcggcatctc
    21601 aagcagattg agtacacgca gcagcgagct tttacccgcg ccgctggggc caagtaacac
    21661 cagcgtttcg ccctgtgggc aatccagcgt gatatcgaac agcgcctgat gcgcgccgta
    21721 gaagcaatta atgccgttta attgaatact cattgacact cgtatactgg cagtctgata
    21781 gctattgagg tcgaagatag tacctttgac agaataatta tgcaatattt atgctttaaa
    21841 agttaaaagc aaagcgcatt attcaataaa catagcacaa aataacgggg gcggtggtcg
    21901 gtgagcataa atgtcggcat tcctcacgaa atgccggata atttacgtgg tttattggtt
    21961 gatcaaggcg ttagcgattc tcgatggact gacggagcgt acccgccgtg gcatgaacgc
    22021 taccgcctaa gtagcgcaca tcgtcgatga cccaacactg gccttcctga atcattaaca
    22081 cttcatcctg ccagccctgg tcaccctgtt tgagatccac ccgcaatgga atgttacggg
    22141 catcacgatt agggatagtc gatgcactgg caacgtgggc gctgtctggc aaggtggttc
    22201 gactggagaa cggatcgctg cttaacaatt cacgatgact attatcgcgg ctggcatcgc
    22261 taagttgggt tgccagtttg tcactcaaat aggggcgcag ggcggtaatg tcattgctgc
    22321 gatgttgaat gcggtagtcg tagaattgtt gagccacact gtcaggccct ccctggacac
    22381 aggaaccggt gcgggggccg ttatctttat aagctggcgt gacggtggta caggcactca
    22441 gcagcagtgc acatggaata agtagagata gtgaacggta gcgcataatc atttccttat
    22501 tagcttgctg tctgcgtcac actacgacca aacgctatca gattatttaa tacattgttt
    22561 ttaatacaat tctgaaacaa tctgaacacc tgattgggtt tgagtccatt gctggctacc
    22621 cactcaaggt tgctcgtagc tgacttactt aaattaccca aacaatagac agtgtaaacc
    22681 atttgtgagg ttgagcgtgt tatatggctg aaatgatcat ttaaggtaaa tatgatagtt
    22741 ctgaaatggt ggtataaatc ctcctgtaga ggttaagagc atctgcaaga agccagtttc
    22801 acatttataa cgcgtgataa atgaatgcgg ttattgtcta atcattacaa aatttttcac
    22861 atgcgataag agatcctgcg attaagatga ttgccaaaat tacgttgtat ttttgaaaat
    22921 gagcgaaatt tgatagtccc agctttgact ctcgataata ttatttatgc tacaattgtg
    22981 ggtaaagaat ttaagctaat gaatttgagt gaagaataaa ttaaattgct tcagtatttc
    23041 gttaattaat atgaatacaa aaggtgttgt gcttttataa ttataaagcg cgaggagttc
    23101 ggtggattat cgtttttctt ggatgatttt taatatgata aacaatatat ctgatcaagc
    23161 atcatcattt ccaggcacac aactaaatca aagtgataat ttcctggatt cactgaggga
    23221 atttttcgcg atattaaatt ccagtcgtaa gggtgagttg tctacatggg ataccatcta
    23281 cttgcatctg atattagcaa taaatgctga cagtgatctc ataaaaaatg atgtgttgct
    23341 cgcggaaaat attccatcag cgaattacca atttaataca ttttttagca atgctttcga
    23401 gattgatgtt aaaaaatatc tgggtaaatc agaagataac gaagttgaaa taaaagcggg
    23461 taatgaaaga atctctattg gaatacgtaa tgtatcgaat ggaaaacttg aaagacaaca
    23521 gtttcttttc ccactggact atgagaataa attacaagaa caattagata agtattttac
    23581 gattgaatcg cacccattat tatacaggta taccattggt agtaagattg cgaatgtgat
    23641 ttttgaaaag ttatacagtc gtattgactt taacaaagaa caatatatat cctttataaa
    23701 ggacgcattt attcattttt atgattatag caggcgatat gctattagtg agaatatcga
    23761 taaagacgct gtaacaaaca atatcgcatt gatgagcact ttttatgaca gtgataatac
    23821 gtcaggtgag gttttaaata atgattttac agaagaagag tcatttgaaa ctgcgttaga
    23881 tgtagagcat gcaatagtgc ttggatttgc tgatgataat tttgaaacaa aacctgttca
    23941 ttatcaggat ttattaacac gatttagtgc ctttcaagat accgtgttta atctgtttcc
    24001 tgaaatgcac tcttcgcatt accacgatat ttgtagcgta agtgtggata tgacaaaggg
    24061 aacgcaatgc atgattcact tgatggtgaa tgaagaagtt ttcatgtctc tgcctgttcc
    24121 agttgccacg atggtacgtg aagatgcaag taatttagtc aatcttaaaa cactactcaa
    24181 tgatggttgt ttcattaaat atagtcattt taatgacgtg gcattaataa agcaaaacat
    24241 aagtaatctc tatttatcac acactgttgt gaatgaaagc atattgaaaa aatgttgttt
    24301 tgaaaacggc agtttaggtg atgttaaaat aacaaatagc aatgtaataa acagtgcatt
    24361 caaaaatatt tcgtttagat ctgttaaaat taataacgta aatactcatt ctttaaaatt
    24421 tattaactgc aattttttca atgttgatat gataagagtt aatctcagca aatgtctttt
    24481 tcatgaatgt agtatgcatg gtgtcaagat aaaaccctgg ttgcctgtaa aatggacaaa
    24541 agaattaata agtgattatt tgtatggctg tttactatct ctgtactcta tctgtgcaag
    24601 ggatatatat aatatgaacg ccgggaataa cgtgaaggtt gctgcggatg ctttccttga
    24661 aattattttc tctcttaaaa ataaatattg tattaaacta ttaagcgctc aggatcgggc
    24721 gtttatatac gagtttgcaa gaatgatctt cgcttatatt aatgacaaaa gcattgagat
    24781 cttattactt tcttgttttg cggcggctga ccaaaaagca atccagcggt atagacctca
    24841 gtcacaagat ggggaagact ttaggagtca tttacaatat aagttgcctc tttccgctca
    24901 ttaatgttgt tggtgcaaca taataagaca ggtaagcggt accctataat gaaaccaata
    24961 tcgggttatg caacggataa tcgtaaagac ttaagtgggt gtttttgaca caatgagatg
    25021 atttcctaaa ctgatcaaaa ccttcttccc aacatggcat atcagtccga taatgcttta
    25081 gagatcgaag gcttaacaaa caaggagatc gatcatgcaa ttttctacaa ccccaactct
    25141 ggaaggccag accatcgttg aatattgcgg tgtggtgacc ggcgaagcga ttttaggtgc
    25201 caatattttc cgtgatttct ttgccggtat ccgcgatatc gttggcggac gttccggtgc
    25261 gtatgaaaaa gaactgcgta aagcacggga gatcgccttt gaggaattag gctcccaggc
    25321 gcgggcgctg ggggccgatg ccgtagttgg tattgatatc gactacgaaa cggtcgggca
    25381 aaacggcagt atgctgatgg ttagcgtcag cggtacggcg gtgaaaacgc gtcgatgaga
    25441 agagtcttct ggctggtcgc tgccgctctg ttactggcag ggtgtgcagg cgaaaaaggc
    25501 attgtcgaga aagagggata tcagcttgat acccgacacc aggcgcaggc ggcgtatccg
    25561 cgcattaaag tgctggtgat ccactacacc gcagatgatt ttgatagctc gctggcgaca
    25621 ctgactgata agcaggtcag ctcgcattat ctgatccctg cggtgccacc gcgatacaac
    25681 ggtaaaccgc gcatctggca actggtgccg gaacaagaac tggcctggca tgcggggatt
    25741 agcgcctggc gcggggcaac gcgccttaac gacacctcta ttggcattga gctggaaaac
    25801 cgtggctggc aaaaatcatc cggagtgaaa tattttgccc cgtttgaaca ggcacagatt
    25861 caggcgctta ttccgctggc gaaagatatt attgcccgtt atcacatcaa gccggaaaac
    25921 gtagtggcac atgcggatat cgcaccgcag cgcaaagacg atccggggcc attatttccc
    25981 tggcagcaac tggcgcagca ggggattggt gcctggccgg atgcgcagcg ggtgaacttt
    26041 taccttgccg ggcgcgcgcc gcacacaccg gtagataccg cgtcattgct ggagcttttg
    26101 gcgcgctacg gttatgacgt aaaacctgat atgacaccgc gcgagcagcg gcgcgtgatt
    26161 atggcattcc agatgcattt ccgcccgacg ttatataacg gcgaagcgga tgcagaaact
    26221 caggcgattg ccgaagcatt gctggagaaa tacgggcagg attagcgcgg cagttttccg
    26281 tggtcgcgta gccaggcggc agttttctcg atgccttcat ccagggtgat gaccggctga
    26341 taacctaact cttcctgcgc acgcgtaata tccagcgtaa agtcaaaatt caacttggag
    26401 acgccgtagt gggtcagcgg cggctctttt gctgacttgc ggcctaaacg ctccatgctg
    26461 tgggcgatca tatccagcat cgggtaaggg acggaacgaa tacgacagtc aatattcaac
    26521 tcgtcgatca gcttctgcac gatgctgcgc agtgtgcgat gctcgccgtt ggtgatgttg
    26581 tacacacgcc cggaaggtag cttatcgcag gcttcctggc ttgccagcca cattgcgtgc
    26641 acggcatttt catagtaggt catatcgacc agcgcactgc cgccatgcgg taacagaata
    26701 ctgccgtagt ggtgcatcat atgcgccaga cggggaataa agactttatc gtgcggtccg
    26761 aacagacttt gtgggcgcag aatagtaaag cgcgtttgtg gattcgcctg cgaaagcata
    26821 ttgatcactt cttcgctggc tgctttgctg cgggcaaact cgttggcgaa gcggtgaggg
    26881 cgaaaatctt ctttaatatc gcgatggtgg tgataatcga agtacaggga gggggaagag
    26941 atatgaataa agttacgcac accccaggcg acagcccatt cacccaggcg gcgagtggcg
    27001 cgaacgttag ccagatcgaa agcctgttgt gtcccccagg gtgaggtaaa gctggagcag
    27061 tgccacagcg tatcaatgcc cgcgagcatc actttagctt gtgatgaaac cagctcggtc
    27121 agatccgtca gaacaaactc tgcgcccatt ttttccagca atttacccat tgcttcgttg
    27181 cgaccgctcg cacgcacgct gatgcctttc tggcataaaa actctaccgc gtttcgacct
    27241 aagccgctgg tggcgccggt aaccagtacc ttcatatcaa tccactgttg ttgagaaaat
    27301 gacgtgcgca ttcttccgtg atttccccca tgatgcaatg ggaaacatga aagaataacg
    27361 caggttttgt cgattaatct gtgctttgtt ctgccagtct ggcgatttgt tttgccattc
    27421 cgcgaaaaat aaacagatgc gcggggatca tcaataacca gtaaaacagc cccggcatac
    27481 cgtgcggatg ccagaaagcg cggacatcga tagtacgata gtcaccttta tcttccaggg
    27541 taaaacacag tcgtcccagc cccggcgctt tcatgccaaa taacaacgta agttgctttt
    27601 tcggttcaac gacaatcact ttccagctat ccaccgcatc gccggtctgt aaatattcgc
    27661 gctccgggcg gcctttcgcc agattatgac cgatcgcgcg gtccatcaac gcccgtgtct
    27721 gccacaaaat attgccaaag aaataacgct ctttaccgcc gatttggttc accaccagcc
    27781 ataaagccgc aaggctggcg gacgttttaa cggtaaaccc cgcctgtttg gcgaaataac
    27841 cgtactccgg tcgccagcgg gcaaaggcct gagcgtcgta gccccagtcg ctggagttga
    27901 ccagtttttc ttcctctttc aacgtgctac gtaccgcgtc atcgaaagcg atcagccgtt
    27961 gtgggatgag tgcacgtagc gcggtatcat ctgccagcag atcgtgtttc agcccctgaa
    28021 tcaacgccct ggcggtggtg ggcggtacgg aagtaatcac attgagaaac cacaccgaaa
    28081 tccagcgggt ggggaggggg atggggatca accagcggcg cttaccgctc accgccataa
    28141 aatgttcaaa ctgttgctga taactgagca cctctggtcc ggcggcttcg aagatgcggt
    28201 gtttgttggc tggatggtct aacagcgcca ccagatagtg cagcaagttt tccagcgcga
    28261 tgggcgtggt gcgtgaacgt acccagcgtg gcggcgttaa cactggcagg ttgtagacca
    28321 tatcgcgcat gacttcgaac gccgctgaac ctgcgccaac gataattccg gcccgaagtt
    28381 cggtcactgg tacattcgct tcacgaagaa tgtccgccgt agcctgacga gctcgcagat
    28441 gatccgactg ctcatgtggc ggggcctgca acgaactgag aaagattaat tgcttaactg
    28501 gtacttcacg tagcgcatcg cggacgttga gagccacctg gcgctcctga gcgataaaat
    28561 cgccgccttc gcccatgctg tgcaccagaa aatagacggt atcgatatcc tgcaacaggg
    28621 caggaaggtt atccggccag ctgagatcga ctttatggca actgacgttt gccagttgca
    28681 gctttgcaag cctgtcgaca tgacgtgccg ccgccaggat ctgatgccct tgctggctga
    28741 gtgtgcgcac cagatgctga ccaatgtagc cactggcacc gagaactaaa atgcgttgcg
    28801 gcacgtctct ctccttaacg cgccaggaat gcgcgccagt gggcggcgac ttccgccagt
    28861 tgttcgcgcg agacgtcaag atgcgtcacc aggcggacaa tcggcgaggc gttaatcagc
    28921 acgtttctcg ctttcatgta ttcgcctaac gcggcagcat tttcttcccc gacgcgaaca
    28981 aacagcatat tagtgtcctg acgcatcaca tccgcgcctg cttcacgcag ttgctccgcc
    29041 atccaggcgg cgttgtcgtg atcttcctgc aaccgtgcga cgttattttt cagggcatac
    29101 atcccggcgg cagccagaat cccggactga cgcatcccgc caccggccat tttccgccag
    29161 cggatggcac gtttaatgta atcacgatta ccgacgagta atgaaccgac tggcgtccca
    29221 agacctttcg acaggcaaat ggtgaacgaa tcacaatatt gcgtgatctc tttcagttcg
    29281 cagccgtaag ccaccacggc attaaagatg tgcgcaccgt caacatgcag cgccagattg
    29341 cgctcgcggg taaattccca tgcttctttc aggtattcac gcggcagcac tttgccgttg
    29401 tgggtgtttt ccagactgag taatttggtg cgggcgaaat ggatatcgtc gggtttgatt
    29461 ttcatcgcca ctttatccag cggtagcgtg ccgtcggcag ccgcgtcgat gggttgcggc
    29521 tgaatactgc ccagcaccgc tgcgccaccg gcttcaaaca gatagttatg cgcggcctga
    29581 ccgacaatat actcttcgcc acgttcgcag tgactgagca gagcgaccag gttggcctga
    29641 gtgccggtag gcagaaaaat ggcggcttct ttaccggaaa gctctgctgc atagtcctgc
    29701 agagcattaa cggtagggtc gtctccgtaa acgtcgtccc caaccggggc ggccatcatc
    29761 gcttcgagca tggcgcggct cggtcgggta acggtatcac tgcgtaaatc aatcatggca
    29821 tgtccttatt atgacgggaa atgccaccct ttttacctta gccagttcgt tttcgccagt
    29881 tcgatcactt catcgccgcg tccgctgata attgcgcgca acatatacag gctaaaacct
    29941 ttggcctgtt cgagtttgat ctgcggtgga atggctaatt cttctttggc gaccaccaca
    30001 tccaccaaca ccggaccgtc gatagagaag gcgcgttgca gggcttcatc aacttcagag
    30061 gctttttcta cacggatacc cgtaatgccg cacgcttcgg caatgcgggc aaagttggtg
    30121 tcgtgcagct cagtaccgtc tgtcaggtaa cctccggcct tcatctccat cgccacaaag
    30181 cccagcacgc tgttattaaa gacgataatt ttcaccggca gtttcatctg cactactgag
    30241 aggaaatcgc ccatcaacat gctaaaaccg ccatcgccgc acatggcgac cacctgacgt
    30301 tccggctcgg ttgcctgcgc acccaacgcc tgcggcatgg cgttagccat cgaaccgtgg
    30361 ttaaacgaac ctaacaggcg acgcttgccg ttcattttca gataacgtgc cgcccatacc
    30421 gttggcgtgc cgacgtcaca ggtaaagatg gcgtcatcgg cggcaaaatg actaatttgc
    30481 tgcgccagat attgcgggtg aatggctttc tcgctcggtt tagctaaatc gtccagccct
    30541 ttgcgggcat cgcggtaatc ttccagcgct ttatccagaa acttgcgatc ggttttttct
    30601 tccaccagtg gcagcaatgc gcgcagggtt gatttgatat cgccgaccag cgccatatct
    30661 accttgctat gtgcgccgat gctggctgga ttaatatcaa tctgaataat tttggcatcg
    30721 gtcgggtaga acgcacggta ggggaactga gtaccgagta gcactaacgt gtcggcgttc
    30781 atcatggtat ggaaacctga cgagaatccg attaacccgg tcattccaac atcatacgga
    30841 ttatcgtatt cgacatgctc tttaccgcgt agggcatgaa caataggtgc tttaattttc
    30901 ccggcaaact caactaactc tttatgcgcc cccgcgcagc cgctgccaca catcagggcg
    30961 atattgctgg aataacgcag cagttgcgcc agtttgcgta actcttcttc ttccggcgtc
    31021 acgactggct gtggcgcatg ataccagtgc gtggttgccc cttctggcgc aggttttaac
    31081 gccacgtcgc ccggtaacac aacaacggaa acgccacggt taagcaccgc tttgcgcata
    31141 gcaattgcca gcacttgtgg gatctgctcc gggctggaaa ccagctcgca atagtgacta
    31201 cattcgcgga atagctcttg tgggtgggtt tcctggaaat agccgctgcc aatttcgctg
    31261 gagggaatat gagcggcaat cgccagtacc ggaacgttat tgcggtggca atcgaatagg
    31321 ccgttgatca agtgcaggtt gcctgggccg cacgatccgg cacagaccgc cagttctcct
    31381 gaaagttgtg cttcagcgcc agcggcaaac gccgccactt cttcatggcg ggtggacatc
    31441 cactcgatgg tgcccatgcg attaagactg tcgctaagac cgttcagaga gtcgcccgtg
    31501 actccccaga tgcgtttcac gcctgccgat tcgagtgttt tggcgatata agctgcaacc
    31561 gtttgtttca tggttctcca tctcctgaat gtgataacgg taacaagttt agttcatctg
    31621 acggaggggg aagggatggg agagaaagga ggcactaacg gttaaatagc ccgatgaaag
    31681 gaatatcatc gggcataagg cgattatgcg agaaccaaat ccccctgcgg atggcaggag
    31741 caggccagta cgtaaccttc agcgatttcg gcgtcggtca gcgtcattgt actgctcacc
    31801 gtatattcac cggaaaccac ttttgtctta cagcagccgc aaacacccgc acggcaggca
    31861 gcgacaaccg gaacgttatt gctttccagc gcctccagta gcgtggtgcc aaccggggcg
    31921 taaaattctc gtgccggttg cagtttggtg aatttcagac cgctggtcgc cgcttctgct
    31981 actggggtga agaatttctc tttaaagaaa cgcgtcacgc cgagcgcttt cacttcctgc
    32041 tctacccaat ccatatacgg agccgggccg caggtcatca cggtacgtga agctaagtca
    32101 ggtacatctg ccagcagttc gcgagtgaga cgaccagcga taaagccttc ggtaacgtta
    32161 ttttctgcca ccagcgttac cggatagtta cgccactcat cggcgaaaat aacatcctgc
    32221 ggcgtacgca cgttgtagat cacccgcaca tcggcctgtg gacggttctt cgcaagccag
    32281 cgacgcatcg acataatcgg cgtgacgccg cagcctgccg ccagcaacag gaatttatct
    32341 tctgctttat cgtcgcaggt aaattccccc atcgcgtccg aaagccagag ataatcaccg
    32401 cgttttacat cgcgcgtcag ccactgggag ccgacaccgt catcaatccg ccgcacggtc
    32461 agggtgatat attcactcac gcctggcgtg gaggaaatgg tgtaagcacg cagcgtttcc
    32521 gctgagtttc gcacgctgac cagtgcatat tgcccggcgc gatatgggta gtgatcgtgg
    32581 caaatcaggg aaatcgtcca cacatccggc gtttcttgcg taatgtgatg aacctgcatc
    32641 cgccacgggc attgattcgt tggcatcgtc atcgacaaac tccttacgcg ctcaacagtt
    32701 gcttcatgtc ttcttcaaca gtggtgatag aacgcaggcc gaatttctcg ttcagcaccg
    32761 ccagcaggtc tggtgtcagg aaactaggtg cagtcgggcc ggtgacgata tttttcacgc
    32821 ccagagaaag cagcgtcagc agaatgacaa tcgctttctg ttcaaaccag gagagcacca
    32881 gcgacagcgg cagatcgttc acaccacagc ccagtttctc tgccagagtg acagccagaa
    32941 taattgctga gtaagcatcg ttacattgac ctgcatctac cagacgcggc agaccttcga
    33001 tatcgccaaa ctcaagtttg ttaaagcgat atttaccaca ggcgagggtc aggatcaggc
    33061 agtcatccgg cacgctggtg gcgaaatcgg tgaagtagtg gcgctcgccg cgtgcgccgt
    33121 cacagccacc aagcaggaag atatgacgca gtttttcacg gctaaccaga tcaatcagcg
    33181 tatcggctgc accaagcagc gtctggcgac caaagcccac ggtgatcagg tgaggaattt
    33241 cgctgtacgg gaagcccgcc atttgttgcg cctgggcgat gaccgcagag aaatcttcgc
    33301 catccagatg acgcacgcca ggccagccaa cgatgctgcg ggtccagata cgatcgtcat
    33361 aagcgcctac ggttgggtcg atgatgcagt tcgaggtcat cacgatgggg ccagggaaac
    33421 gagcgaactc cacttgctga ttctgccagc cgctgccgta gttaccgacc agatgcttga
    33481 atttacgcag ctccgggtag ccatgcgcag gcagcatttc gccgtgggtg tagacattaa
    33541 cgcccgtgcc ttcggtctgt tccagcaggt tgtagagatc tttgagatcg tgaccggaaa
    33601 tcagaatgca tttacccgcc gtcgctttga cgttgacctg ggttggcgtc gggtgaccgt
    33661 atttaccggt ttcgcctgca tccagaatgc tcatcacttt gaagttcatc tggccgattt
    33721 ccattgaaca ctcaagaagc gcgttcatat cggcaggcca ggtccccagc cacgccatga
    33781 ttttatggta ctgggcataa atatcgttgt cgtattgacc gagaacatgc gcgtgttcca
    33841 tataggccgc cgcacctttc aggccataca ggcacagcag acgcaggccg agaatgtttt
    33901 cgccaatcgc cgctttatct ttgttagggg taaattctgc tgcctgacgt tgcagctcgc
    33961 cgagatcatc gctcaccagt tgcaggtcag ccatcgggtt atcgacgcgc gcgttggcat
    34021 ctacagccag gcattgtgct ttcagcgcct cgcgcaaggc aatcgcttca cgagcgtagc
    34081 cgacaatacg cggagaatcg aagttaacgt tggtcagggt tgagaaaaag gcacgtggcg
    34141 cgaagctgtc aacatcgtgg ttgatgatgc cgtattcacg cgcttttacc gcccaggcag
    34201 aaagcccttg cagcgccgcg atgagtaaat cctgaaggtc agaagtttcc gccgttttac
    34261 cacacatccc ctgcgcgtat gagcagccgt ttcctgccgg agtacggata gtttgttcac
    34321 attgcacaca aaacatgatc acacctttta aagttatatt taatatacat gtttaaggtt
    34381 aagacgctta acgcggggat aaaagggatt tttcatgcaa ctttaaggga gattgattta
    34441 gcgcaatttt ggcggcaggg atctaccgcc agagaggtat tacgcagaga aaaaggcgat
    34501 gaggatcggc actaacaggc taagaataaa accgtgaaca attgccgccg ggaccatatc
    34561 cagcccgcca gtacgttgaa gaacgggcag ggtgaaatcc attgatgtgg caccgcataa
    34621 gcccagtgca gtagagcggc tgcggcgaat cagcccaggg atcaacataa tagcaatcag
    34681 ttcacgggcc agatcattaa aaaacgccgc gctcccgatt accggaccaa aagattcggt
    34741 caataaaata ccggaaagag aataccagcc gaaaccggag gccattgcca gcgcggtatt
    34801 gatggggaga tcaagaataa aggcgttaat taaaccacca attaatgaac tgacaaccac
    34861 caccacggcg acaatcattc cccggcgatt aaggacaatc tgctttaagg tcatgccatt
    34921 attgcgcaac tgaataccaa cgaggaaaag tagcaaaatt aacgtgtatt cactggcttc
    34981 ggtcgcgtgt tgtaagaaag ccagtccact cagaccaatg gcaaaaccaa tcactactac
    35041 gccgcacagt tttagcgact ccagcgccat cgcaatacgc gacgggagtt tttcttgctg
    35101 atggtggttg cgccacggca ggcctcgctc cagccacatc agggcggcaa tattacacag
    35161 taaaataacg gtaatactga cggcagaata atgcagaatc gccaacaggt tactggcgag
    35221 gttatcgaga aacgccagac tgatacccat aaaaaagaga ataaggtaaa ccatccagct
    35281 taatagctga ttaataactt ttaacgcagc ttgttggcga agcggaatga ggtaacccac
    35341 aatcagggga accagaatga ttaacagccc agaaaacatg aaaatccagt ccttgcaaag
    35401 aagaagtcga aatgcgcgat gacacactac tgaaagcgga aggacgagta aagttgcaat
    35461 taaaaggaaa tgttatgcat cagaggggga tttttaatcc tatctgagtg tttagcattg
    35521 ttgcgctcct ttcatgtgca atgccatata aagccactgg cggagggagc gcgcaggggg
    35581 cagccagtcg ccgccgcccc ctgcaccccc gggctctggc gaacaaaatc gccgctgcgc
    35641 ggtaccttca gcttatcccc ttcggcaacc aggtcgggcg cgaggtaacg tcctgtaaga
    35701 cgcgccctca gcccacatcc atgtgggctg ccccggcctt cggggaacgc ttcagcgatt
    35761 ttgacgccac caatacccgt gcggtttgta atgtcgaaga taaaacaacc agggcagata
    35821 aaatgccaat gctaattaat cacgcttttc cagcagagtc cggtaaatca gaccaccgat
    35881 aatgccgccg acaattggca ccacccagaa gaaccacagt tgttctaatg cccagccgcc
    35941 ctggaagata gcaaccgcgg tgctgcgcgc cgggttaaca gaagtgttag tcaccggaat
    36001 actgattaag tgaatcaggg ttaaggccag accaatagcg atcggcgcaa aacctgccgg
    36061 agcgaatttg tcggttgcac cgtggatcac taacaggaag cccgcactca ataccagttc
    36121 aactaccagc gcggaaagca tggaataacc gcctggtgaa tgctcgccat aaccgttaga
    36181 agcaaaaccg ctggctgccg cgtcaaaacc cgttttacca ctgacaatta aatacagcag
    36241 cgccgctgca acaataccgc cgacaacctg ggcaatcacg tagccaacga cttcttttgc
    36301 cggaaaacgt ccgccagccc ataaaccaat agtgaccgcc gggttaaaat gaccaccaga
    36361 aatatgacca acagcgaagg ccatcgtcag aacggtcaaa ccaaatgcca gcgccacgcc
    36421 ggcaaaacca atgcctaatt ccgggaagcc tgcggccagt acagcactac cacagccacc
    36481 aaaaacaagc cagaaagtac caaaacattc agctgctaat tttctgaaca tatccacctc
    36541 aattaaaaat tgaccctgtg aaaaataggg tcgttttata gggtcgtcgt aaaaaatgac
    36601 gacggaaata atgcgcggct attttaaaaa cgaaggcgag tcattcacca gataaataaa
    36661 tccagtaaat ttgatttagg gcaacagcgg gttgccccat atagtcattt gtctgattga
    36721 cagtgtagtg caagcaaaag atttaatcct ttaggcgtaa taaaaaataa tttatcatgc
    36781 taattatttg attttgttgt ttttgcgggc ttatcagcaa gagggagtat aacgcgatta
    36841 ttcgctcatt tttcagacaa ttgccatgct taaatgtgat gtcatcacgt attagcaagg
    36901 cctttcccgt tatactgcca gcgtaaagga taagtcacat atttctggag gggatatgat
    36961 tcttgagcgc gttgaaattg tgggttttcg cggtatcaac cgtttgtcgt tgatgctcga
    37021 acaaaacaac gtcctgattg gggagaacgc gtggggtaaa tccagcttgc tggacgcctt
    37081 aaccctgctg ctatcgccag aatcagatct ctaccatttt gagcgcgacg atttctggtt
    37141 cccgccggga gatatcaacg ggcgagaaca tcatctgcat attattttga ccttccgcga
    37201 atcgctgcca ggccgacatc gggttcgccg ttatcggccg ctggaagcgt gctggacgcc
    37261 atgcaccgat ggctatcacc gtatttttta tcgtctggaa ggggagagtg cggaagacgg
    37321 cagcgtgatg acactgcgca gttttctcga taaagacgga catccgattg atgttgagga
    37381 tattaacgat caggcacgcc atctggtgcg tttaatgccg gtgctgcgct tgcgtgatgc
    37441 ccgttttatg cgccgtattc gtaacggcac ggtgccaaat gtccccaatg tggaagtcac
    37501 cgcgcgccag ctcgatttcc tcgcccgtga gttgtcctca catccgcaaa atctctctga
    37561 tggacagatt cgtcagggac tttccgcaat ggtgcagttg cttgagcatt atttctctga
    37621 gcagggggct ggacaggcgc gatatcgttt aatgcggcgg cgggccagca atgagcaacg
    37681 aagctggcgc tatctggaca tcatcaaccg gatgattgac cgacctggag ggcgctcgta
    37741 tcgggttatt ttgctcggcc tgtttgctac tttattgcag gcaaaaggca cattgcgact
    37801 ggataaagac gcgcgtccgt tattgctaat tgaagatcca gaaacccgcc tgcatccgat
    37861 tatgctttcc gttgcctggc atctgttgaa tcttttgcct ttacagcgta ttgctaccac
    37921 caactctggc gagctgcttt cgttaactcc agtggaacat gtttgccgcc tggtgcgtga
    37981 atcttctcgt gttgccgcct ggcgtctggg gccaagtggc ttgagcaccg aagacagccg
    38041 ccgcatctct tttcacattc gttttaatcg tccgtcatcg ctgtttgctc gctgctggtt
    38101 gctggtggaa ggggaaacgg aaacctgggt tatcaatgaa ctggcgcgtc agtgcggaca
    38161 tcattttgat gccgaaggga tcaaggtcat tgagtttgcc cagtccgggc taaagccact
    38221 ggttaaattt gcccgccgaa tggggattga atggcatgta ctggtcgatg gcgatgaagc
    38281 agggaagaaa tatgccgcta cggtacgcag cctgttgaat aacgatcggg aagccgaacg
    38341 agaacattta acggcgttac cggcgctgga tatggaacat tttatgtatc gccagggatt
    38401 ttccgatgtg ttccaccgcg tggcgcaaat cccggaaaat gtaccgatga atctacgcaa
    38461 aattatctcg aaagcgatcc atcgctcttc caaacccgat cttgccattg aagtggcaat
    38521 ggaggcagga cgtcgtggtg tggactccgt accaacgctg ctgaaaaaaa tgttctcacg
    38581 cgtgctgtgg ctggcgcgcg gtcgcgcgga ttaaccgcga aacatcgtgg ccatttgtgg
    38641 ctgaatagcg tcgagcatct catagcgccg acggtattca gcccgttttt tactggcgat
    38701 ttcggcaatc tcttttcgtg ctatctgtgc tggaaggcgg taatggcgtt cagcatcaca
    38761 tacgccgcca accgactccc agaacgcgtt gtaatcagca tggatcttgc cttctttatc
    38821 gcgataacgc aggctgcggt aaatatgcgt ttcattgctg acggcaataa tctgctctac
    38881 ctgcaaacgt tgggcaaaca gacaggccgc ttccatcacg aggcgtttgg gaaatagccc
    38941 gtggcaggct ttcgtcgcat tctggatttc ctgatgtgga atttcccatt ttgcgccttg
    39001 cagtccgcca ataaacatcg ttcttttccc ctgatattca cacagggtaa acgtgatctc
    39061 tgccagagga ataccttcgc tgttgcggaa caggattgtg ctgtcacctt ctttatccat
    39121 tgagatcatc atggtcagct caagcgtgaa ctgctcaccg tttttgcctt ccagtttagc
    39181 cagttgcagc ccgggggtat tcaaatataa gctgaattct tccgccgaca tacacccgcg
    39241 gagtaacgca taatggtaac gtaacgcctc cagcaattgc ttacggctaa gattcgccgc
    39301 aaggtaaggg cgatgcagac gcacaggcag tcgcggctgg cgcgttaaca acacattgag
    39361 attaggccag tgggaaagtt cgttcatcca ctcaacgctt aaacgcggca taatcaacga
    39421 gcgcagcaaa aatttctggc gaaaactaca gcgatgccag aatttacccg gccgacactg
    39481 tccacgtgcc agactaagaa aaagtgacag gctgctgaga gattcagatg gcgtaaaggt
    39541 ccgttcagtt agctgcgaca tattcatgaa atcaatggtt atacatgacg tcgatttcac
    39601 cattgcgtat cttaaccaaa catcaatagt gtgattacta acgtaaattt tagggttttg
    39661 ttgatatttc gttgaagtta atgacccgga ttggcatgtg gagtattcag aatatttatg
    39721 aaaaagcgga aaaccgtgaa gaagcgttac gttattgcgc tggtgatagt catcgccgga
    39781 ctgattacgt tatggagaat tcttaacgca cccgtgccga cttatcagac actgattgtg
    39841 cgccccggtg atttacagca aagcgtgctg gcgaccggaa agctggacgc gctgcgtaag
    39901 gttgacgtag gcgcgcaggt cagcggtcag ttgaaaacgc tgtcggtggc gattggcgat
    39961 aaagtaaaaa aagaccagct tttaggggtc attgatcctg aacaggctga aaaccagatc
    40021 aaggaggtcg aagcaacgct gatggagcta cgcgcgcagc ggcagcaggc ggaagcggag
    40081 ctgaaactgg cgcgggtgac gtattcccgt cagcaacgtc tggcacaaac gcaagcggtt
    40141 tcactgcagg atctcgatac cgccgcgacg gagatggctg tgaaacaggc gcaaattggc
    40201 accattgacg cgcaaatcaa gcgcaatcag gcctctctcg atacggctaa aaccaatctc
    40261 gattacaccc gtatcgttgc cccgatgggc ggggaagtga cgcaaatcac cactctgcaa
    40321 ggccagacgg tgattgccgc acagcaagca ccgaacattc tgacgctggc agatatgagc
    40381 actatgctgg taaaagcgca ggtttctgaa gcggatgtaa tccacctgaa gccggggcaa
    40441 aaagcctggt ttacggtgct tggcgatcca ctgacgcgct acgaggggca aatcaaggat
    40501 gtgctaccga cgccggaaaa ggtaaacgac gctattttct attacgcccg ttttgaagta
    40561 cccaacccca atggtttgct gcggctggat atgaccgcgc aagtgcatat tcagctcacc
    40621 gatgtgaaaa atgtgttgac gatccctctg tcggcgttag gcgatccggt tggcgataat
    40681 cgttataaag tcaaattgtt gcgtaatggt gaaacacgcg agcgtgaagt gacgattggc
    40741 gcacgtaacg ataccgatgt tgagattgtc aaagggctgg aagcgggcga tgaagtggtg
    40801 attggtgagg ccaaaccagg agctgcacaa tgacgccttt gctcgaatta aaggatattc
    40861 gtcgcagcta tcctgccggt gatgagcagg ttgaggtgct gaagggcatc agcctcaata
    40921 tttatgcggg tgagatggtc gcgattgttg gcgcttcggg ttccggtaaa tcgaccctga
    40981 tgaatattct cggctgcctg gataaggcca ccagcggcac ctatcgcgta gccggtcagg
    41041 atattgccac gctggacgcc gatgcgctgg cgcaactgcg ccgcgagcat ttcggcttta
    41101 ttttccagcg ttaccatttg ctttcgcatt taaccgccga gcagaacgtt gaagtacccg
    41161 ccgtctatgc cggtcttgag cggaaacagc gactgcttcg tgcccaggag ttgctgcaac
    41221 ggctggggct ggaagaccgt acagagtatt atccggcaca gctttcgggt ggtcagcaac
    41281 agcgcgtcag catcgcgcgg gcattgatga acggcggtca ggtaattctt gccgatgaac
    41341 cgaccggcgc actggacagc cattctggcg aagaggtgat ggcgatcctg catcagctgc
    41401 gcgatcgcgg gcatacggtg attatcgtca cccacgatcc gcaggtcgct gctcaggccg
    41461 agcgggtgat cgaaattcgc gacggcgaaa ttgtgcgcaa tcctcccgcc attgaaaaag
    41521 tgaatgttgc tggcgggacg gagcctgttg tcaatacagt gtctggctgg cggcagtttg
    41581 tcagcggttt taacgaggcg ctgacgatgg catggcgggc gctggcagcg aataaaatgc
    41641 gtactttact gaccatgctg gggattatta tcggtattgc gtcggtggtt tccattgtcg
    41701 tggtgggtga cgccgccaaa caaatggtgc tggcggatat tcgttctatt ggtacgaata
    41761 ctattgatgt ctatcccggg aaagattttg gcgatgacga tccgcaatat cagcaggcgc
    41821 tgaagtacga cgacttaatc gccatccaaa aacaaccgtg ggtcgcctca gccacacctg
    41881 ccgtctcgca aaacctgcgc ctgcgttata acaatgttga tgttgctgcc agtgccaatg
    41941 gcgtgagcgg cgattatttt aatgtctatg gcatgacctt cagtgaagga aacaccttta
    42001 atcaggagca gctgaacggt cgtgcgcagg tcgtggttct cgacagtaat actcgccgcc
    42061 agcttttccc ccataaagca gatgtggttg gcgaggtgat tctggtcggc aatatgcccg
    42121 ccagagtcat tggtgtggcg gaagaaaaac agtcgatgtt tggtagcagt aaagtgctgc
    42181 gtgtctggct accttacagc acgatgtccg ggcgagttat gggccagtcg tggcttaact
    42241 ccattactgt cagggtgaaa gaaggatttg acagcgccga ggcggaacag caactcacgc
    42301 gtttactttc actgcgccac ggtaagaagg atttctttac ctggaacatg gacggcgtct
    42361 tgaaaactgt tgaaaagacc acacgtactt tacaactgtt tctgacgctg gtggcggtga
    42421 tttcgctggt ggtgggcggt attggtgtaa tgaatattat gctggtgtca gtgaccgagc
    42481 ggacgcggga aattggcatt cgcatggctg taggtgcgcg agcaagcgat gttttgcaac
    42541 agttcctgat cgaagccgta ctggtttgcc tggtcggtgg cgcgttggga ataacactgt
    42601 cactgttaat tgctttcacc ttgcagcttt tcttacccgg ctgggagatt ggtttttcac
    42661 cgttggcgct gctgctggcg tttctctgct cgacggccac cgggatttta tttggctggt
    42721 tacccgcacg aaatgcggca cgactggatc cagtagatgc tctggcacgg gagtaatttt
    42781 tgagataaaa atgccagccg atcgggctgg cattttgcct ttaggatgta cacaatgaga
    42841 cagaagagct atgcgactgc cgcttctact tcgacgggca caataacact ggcgtgattg
    42901 ccttttggcc cctggtggac atcaaactga acggattgtc cagcttttag cgttctgtaa
    42961 ccatccatct gaatggtgga ataatgagcg aaaatatctt cgccgccgcc ttcagggcag
    43021 atgaaaccaa aacctttggc attgttgaac cacttaacag tacccttttc catgcttcga
    43081 catccttcgc aaatcttata caagtaagat ggaataaacc ggggtcagag agggggctgt
    43141 tcaaaacctc gccaactcta gaaatacaat ttagagaatt agggcgagcc gtcaagcatt
    43201 tgacaggggc cagggggcag gtatgaatca aaaatttgaa gcagttaacg ctattgacag
    43261 gaatgtgaca gatgtcgctg atgccaacga tagatgatag ttatctatca tgtggagtag
    43321 attggtcagg caaataagct cttgtcagcg gcagggcgtt ctgccgataa ccgtaaccga
    43381 agatgataac tgacaatggg taaaacgaac gactggctgg actttgatca actggcggaa
    43441 gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata aagtgatatt agtcaatgat
    43501 gattacactc cgatggagtt tgttattgac gtgttacaaa aattcttttc ttatgatgta
    43561 gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg ggaaggccat ttgcggagtc
    43621 tttaccgccg aggttgcaga aaccaaagtg gcgatggtga acaagtacgc gagggagaat
    43681 gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc aggcataaaa attgggggag
    43741 gtgcctatgc tcaatcaaga actggaactc agtttaaata tggctttcgc cagagcgcgc
    43801 gagcaccgtc atgagtttat gaccgtcgag cacttgttac tggcgctgct cagtaaccca
    43861 tctgcccggg aggcgctgga agcgtgttct gtggatttgg ttgcgctccg tcaggaactg
    43921 gaagccttta ttgaacaaac cacacccgtt ctgcctgcca gtgaagagga gcgcgacaca
    43981 cagccgacgc tgagttttca gcgtgtactg caacgtgcgg tcttccatgt ccagtcctcc
    44041 ggtcgcaatg aggttaccgg tgcaaacgtt ctggtcgcta tcttcagcga acaggagtcg
    44101 caggcggcat atctgttgcg taaacatgaa gtcagccgtc tcgatgtggt gaactttatc
    44161 tctcatggca cgcgtaaaga cgagccgaca cagtcttctg atcctggcag ccagccaaac
    44221 agcgaagaac aagctggtgg ggaggaacgt atggagaatt tcacgacgaa cctgaaccag
    44281 cttgcgcgtg tgggcggaat cgacccacta attggtcgtg agaaagagct ggagcgtgct
    44341 attcaggttc tctgccgtcg ccgtaaaaac aacccgctgc tggtggggga atctggtgtc
    44401 ggtaaaaccg cgattgcaga aggtcttgcc tggcgaattg ttcagggtga tgtgccggaa
    44461 gtgatggctg actgtacgat ttactctctc gatatcggtt ctctgttagc gggcacaaaa
    44521 tatcgcggcg actttgaaaa acgttttaaa gcgttgctca agcagctgga gcaggacact
    44581 aacagcatcc tgtttattga tgagatccac accattatcg gtgcgggtgc agcgtctggt
    44641 ggtcaggtcg atgcggctaa cctaatcaaa ccgttgctct ccagcggtaa aattcgcgta
    44701 attggttcga caacctatca ggagttcagc aacattttcg agaaagaccg tgctctggcg
    44761 cgtcgcttcc agaaaattga tattactgaa ccgtcgatcg aagaaactgt tcaaatcatc
    44821 aatggcctga aaccgaagta tgaagcgcac cacgacgtgc gttataccgc aaaagcggtg
    44881 cgtgcggcgg tagagctggc ggtgaaatac attaacgatc gtcatctgcc ggataaagcc
    44941 attgatgtta tcgacgaagc gggcgctcgc gcacgtctga tgccggtaag caaacgcaag
    45001 aaaaccgtta atgtggcgga tattgagtcc gtggtggccc gtattgcacg cattccagag
    45061 aagagtgttt ctcagagtga ccgcgatacc ctgaaaaacc tcggcgatcg cctgaaaatg
    45121 ctggtcttcg gccaggataa agccattgag gcgctgactg aagccattaa gatggcgcgt
    45181 gcaggtttag gtcacgaaca taaaccggtc ggttcgttcc tgtttgccgg tcctaccggg
    45241 gtcgggaaaa cagaggtgac ggtacagctt tcgaaagcgt tgggcattga gcttctgcgc
    45301 tttgatatgt ccgagtatat ggaacgccat accgtcagcc gtctgattgg tgcgcctccg
    45361 ggatacgttg gttttgatca gggcggtttg ctgaccgatg cggtaatcaa gcatccacat
    45421 gcggtgctgc tgctggacga aatcgagaaa gcgcatccgg acgtgttcaa tattctgttg
    45481 caggtgatgg acaacggtac gctgaccgat aacaacggac gcaaagcgga cttccgtaac
    45541 gtggtgctgg tgatgaccac taacgccggg gtacgggaaa ctgagcgcaa atccattggt
    45601 cttatccacc aggataacag caccgatgcg atggaagaga tcaagaagat ctttacaccg
    45661 gaattccgta accgtctcga caacattatc tggttcgatc atctgtcaac tgacgtgatc
    45721 catcaggtgg tggataaatt catcgtcgag ttgcaggttc agctggatca gaaaggtgtt
    45781 tctctggaag tgagccagga agcgcgtaac tggctggccg agaaaggtta cgaccgggca
    45841 atgggcgcac gtccgatggc gcgtgtcatc caggacaacc tgaaaaaacc gctcgccaac
    45901 gaactgctgt ttggttcgct ggtggacggc ggtcaggtga cggttgcgct ggataaagag
    45961 aaaaatgagc tgacttacgg attccagagt gcacaaaagc acaaggcgga agcagcgcat
    46021 taatctgatt gtcaggtagg ttggtgaagt ccgtaatctc gtcaggggtt acggactttt
    46081 tatttatggg gtggaacggt gaaaacccta tttttggagg tgaaggtaag ttgttgataa
    46141 ttagtgctgc tggaaggtaa ggataaaaaa gggtgctgca ggagaatggg atggttttgc
    46201 tttattaaca acgggctaaa cgtgtagtat ttgagttcac tgccgtacag gcagcttaga
    46261 aatcgacggg gtgcggtaaa acctttgcga acgcgttcac tgccgtacag gcagcttaga
    46321 aattcacagg taacatactc cacccgccca ccatgttcac tgccgtacag acagataaaa
    46381 tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt aaatatggcg gtgagggggg
    46441 gattcgaacc cccgatacgt tgccgtatac acactttcca ggcgtgctcc ttcagccact
    46501 cggacacctc accaaattgt tttgctacca aacctcatgg gtggcaacgg ggcgctacta
    46561 tagggagttg gagtaaaacg gtcaagaaga attttaatga taattattgt ttgctcatac
    46621 tgtaaacaac ttgtgcagta tatctacatc gagacaagtt atggacttat acttccaaag
    46681 tacttcatac atatcacaaa ataaaaaggc cggttaaacc gaccttttac tcgttctttc
    46741 tcttcgccca tcaggcggta aaacaatcag cgactacgga agacaatgcg gcctttgctc
    46801 aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg tcaggatgcg gatgtagttt
    46861 ttgcgcattt taccggagat gtgtgcagta accacgtgac cgttttctaa ctctacgcgg
    46921 aacatggtat taggcaacgt ttcaagaacg gtaccttgca tttcaatatt gtcttctttg
    46981 gccatctaat cctctggggt atcactaccg taatttgaac cggcaagata atgccgaagt
    47041 tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa acaggttcgg cacattactc
    47101 cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat aagggagcgg tgagataaac
    47161 gatgggcgtt acctgacgcg aaaaattcct tatcggcagc ggggtaatga gcgtaaccaa
    47221 ctctgcgacc gcaattataa cactctgggg agaaatgtgc cgaaaacatt cattcttgtg
    47281 gtgaaaacaa gcaccgtggt acccagaaat tattcggcaa tcgtccgagg cgcatttgat
    47341 tgagataatt aaggtaatcc cggcggggaa tttcgcaggc accaagcgat gctgtgtgat
    47401 cgttaaggac ctggcagtcg ataagcttac cgccatgacc gataaattcc tcacagaata
    47461 ccagaagcgc cgttttagac gcattttcca tccggctgaa catggactcg ccacaaaata
    47521 gcgttccctg ggccacgccg tacataccgc cgacaagctc atcttcacgc cagacttcaa
    47581 tggagtgggc atgaccgagt tcgtgaaggc gatggtaggc ttcgaccacg ccacgcgtga
    47641 tccaggttcc ttcttcgcga tcgctggcac agccttcaat gacctgacca aaagcgtaat
    47701 tcatcgtgac acgatagggc gagcgtttat gaaatcgctt catactacgg ctgatatgca
    47761 gtgattctgg ccatagcacc gcgcggggat cgggcgacca ccagaggatg gggtcgcctg
    47821 gagaaaacca cggaaaaata ccacgctggt aagccattaa caggcgcgca gggctaagat
    47881 cgcccccaag tgccagcagg ccgttaggct cacgtaatgc gccttccggg gaagggaagg
    47941 ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc aaaactccac gcaagtcgga
    48001 tcgttcaata atagcttaca aaccctgctt gaactggtaa taacgcccct gtctggcaag
    48061 cagttctgcg tgagtacctt gctcaataat ttgcccgttg tccatcacta ttatttgttg
    48121 gaaacgagag agtccgcgaa gtcgatgggt gaccattaac accgttttct cacgcatcat
    48181 ttttgcaagc aattcaagga tctggctttc ggttgtggca tctaagcctt cggtaggttc
    48241 atccagcaac accagtggcg catcatgtaa cagcgcacgg gcgatagcca gacggcgcag
    48301 ttcaccaccg gagagctggc gtccgccttc acctaaccaa ctgttgagac ctgcatcctc
    48361 gagcagcttc tccagaccaa cgcgacgcaa gatctccgac agagcctcat cactactgcc
    48421 aggcgaggcg agtaacagat tatcacgcag cgtggcgcta aacagatgca ctcgctgagg
    48481 aacaacgctg atggtctgtc gtagagccgc ttcattcagg ctggctatgg ggctatcgtt
    48541 aagcaaaatc tcgccctgtt gcgggtccca tgcgcgggtc agcagttgta acagtgttga
    48601 tttgccgcat ccggttcgcc cgagaatcgc tatatgttcc ccggcgttta cctgaagaga
    48661 aatcccttta agtgcctgtt gggattgctc cggataagtg aactgaacat cccgtaatgt
    48721 cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg aaggtgacct ccggtttttg
    48781 atccgttaag tcagagatac gtacggcaga ggcaatgact tgccccagat gctgaaatgc
    48841 acccgttact ggtgccagtg cttcaaacgc ggctaacgca caaaagacaa acagggcaat
    48901 taatgcgccg ggttgagcat tgccgccaac gccgccagac gccatccaca gcatcaggat
    48961 caccgctaac gcgccaatga gcagcattat cgcttgcgac aatgcggtca gttcagattg
    49021 acggcgttgc gcttccagcc attgaatttc tgtattctct agttgcgtgc gataacgatc
    49081 gctggcacca aaaatggtca gctcagcttg cccttgcagc caggccgtca gttgttggcg
    49141 atactgtccg cgaagatgag tcagattttg cccggtgctt tttcccgcac gataaaacag
    49201 cggtggcatc aggaaaagcg tcagtaacat aacgctgccc agcgtaaagg cgagggtgaa
    49261 atcaaggaaa cttaacccga ttgtcaccac cataatcacc acaaaagcgc ccaccagcgg
    49321 cgagataacg cgcaggtaaa gatgatcgag cgtatcaaca tccgccacca cgcggttgag
    49381 caattcgccc tgacgatagc gcgccagtcc ggcaggggag aggggcaaca atttgctgaa
    49441 ggtgtaaatg cgcagatgct gcaacacgcg gaaagtcgcg tcgtgactta ccagacgttc
    49501 aaaataacgc ccggcagtac gggtgattgc ggcaccacgc acgcccgcag cgggtagcat
    49561 atagttgaag ctgtacagtc cggcaacccc cgcaaccgct gaggccgaga ggaaccagcc
    49621 ggaaagtgtc aacagaccga tactggcgag ctgcgtcaca attgccagca caataccaag
    49681 acttaacatc catttatgac gtttatacag tgccagatag ggtagcaaag cgcgcattta
    49741 aatctcctcc tgacgatggg ccagtaatgt ggcgaatggg ccaccagcca cacttaattc
    49801 cgcgtaacgt ccttgctcaa taatccggcc atcctgcata acccaaatga catcccagtc
    49861 agcaagatct tctaactggt gggtgaccat taacgttgtc tggcgcagag aggcggcatt
    49921 cagcgcctcc attacgcgct gttcactgtg agcatcaagg ctggcagcgg gttcatccaa
    49981 cagtaatagc gaacagggat ttagtaacgc acgggccacc gccacgcgct gcgcctgccc
    50041 cacggaaagg cgggcagcct ggtcgccaac aggtgtatca acgccttgtg gcaggagcgg
    50101 tagaaactcg ctgacccagg cgttatccag cgctgcttgt aattcttgtt cgctggcatc
    50161 aggtcgcgcc agtagtacgt tatcccgcaa ttttgctgcc ggtaattgtg ggttttgccc
    50221 aacccaggag agatgtttac gccatgattc tggtgataaa tcacgtaatt ctatcccgtt
    50281 gattcgtaat gatccctgat atgagagaaa accagaaagc gcgttcagca gtgagctttt
    50341 acctgaaccg ctgcggccaa ccaacactgc acgttggcct gctggcaaag taaagttcag
    50401 cggtccggcc agcgttttac cttccggcga cgtgataaac agatcctcgg cctcaatggt
    50461 cagcggatcg gtcaatgcta attccgcctc accgcgctgc ggatgggcga gcggggtttc
    50521 cataaacgtt ttcagactgt cagctgcacc aacagcctgg gctttagcat gataaaacgt
    50581 accgagatcg cgtaatggct ggaaaaactc tggcgcaagg atcagggcca gaaaacctgc
    50641 agccagcgtc accccagtat cgtagtgacc aaaatccagc tcgccgagat aggaaaaacc
    50701 aaagtagacc gccaccagag caattgacag cgaggtaaaa aattcgagaa tgccggagga
    50761 taaaaacgcc agtcgtagca cttccattgt ccgttggcgg aaatcttccg aagcagaacg
    50821 aatactttca atttcagctt caccacgacc aaaaatacgc aatgtttcca tgccgcgcag
    50881 gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga aagttacgcc ggttagcatc
    50941 ggcagccccc attccaacca gcgccataaa caacggaatt agcggtgctg tgcccagcag
    51001 aatgagcgcc gcagcccagt tagaggggaa gatcgccacc acaatcagca acggcaccga
    51061 cactgccagc gccatttgcg gcagatagcg tgcatagtaa tcatgcatat cgtcaatttg
    51121 ctcgagtacc agcgtcgccc agctccccgc aggtttaccc tgaatccacg ctggccctgc
    51181 ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag cggatatgct gcccggcgtg
    51241 ataacccacc cgttcgcgta accagaccac ccatgcgcgc aatacaaagg tcagaaccag
    51301 taacgtaaag ggaagcagca gggcttcacg gggaatattc tccataatca tatgttgcag
    51361 aattcgcgcc atgaaccagg cctgggcaat gatcaatatg ccgcttacaa agcccagcag
    51421 acgagaaata ttcagccaac gttgggagat gacgctttgc tgttttaacc agcgggttaa
    51481 ctctttttga cgagatttat tcattgcacg cttagcaggt gagttatcag aattatttgc
    51541 agagcaatgt tacaacgggg aaaaaataaa ggcgacccat agtcgcaagg tgtcgccttc
    51601 tttacttttg ttactgattt gtaaaattat tttgcgtcag ctaaaccatc gaggtagcgt
    51661 tccgcatcaa gtgctgccat gcagccagta ccggccgaag taatggcctg gcgataaatg
    51721 tgatccatca cgtcgcctgc ggcaaagacg ccaggaatgc tggtctgggt ggcattacca
    51781 tgaatacccg actgtacttt gatgtagccg ttttccagtt ccagctgccc ttcgaaaatc
    51841 gcagtattcg ggctgtgacc gatagcaaca aacagaccgg caacgtcgag tgactcgatg
    51901 ttatcgctgt tttgcgtatc gcgcagacga acgccagtga cacccatttg atcgccggtc
    51961 acttcttcca gcgtacggtt ggtgtgcaga atgatgttgc cgttctccac tttatccatc
    52021 agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac ggcggtgaat cagatgcact
    52081 tccgaagcga tgttagacag atacagcgcc tcttcaaccg cggtattgcc gccgccgatg
    52141 accgcaactt tctggttgcg atagaagaaa ccgtcgcagg ttgcacaagc agaaacccca
    52201 cggcctttaa aggcttcttc agagggcagg ccgagatagc gtgcagaagc tccggtggca
    52261 ataatcagcg cgtcgcaagt gtattcgccg ttatcgccat tcagacggaa cggacggttt
    52321 tgcagatcca ccttgttgat atgatcaaaa atgatctcag tttcaaactt ggtggcatgt
    52381 tcgtgcatgc gcgccattaa taacggaccg gtcagatcgt ttggatcacc aggccagttt
    52441 tccacttccg tggtggtggt cagttggccg cctttttcca tgccggtaat cagcacaggt
    52501 tgcaggttgg cgcgcgccgc gtagacagca gcggtgtatc ccgccgggcc tgaacccagg
    52561 ataagcagtt tactgtgttt ggtcgtgccc atgagatccc catagttgtt ggcagacaat
    52621 gggcaggatt gtagggaatt tacagacgta aaaaaagagt atgacgattt tgttaacaat
    52681 ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc cgtcattatc acctctgcta
    52741 cttaaatttc ccgctttata agccgattaa atgatgaata aacgcccctg ttaatgaata
    52801 tctggcatgt tgtactaaaa attgatgttt tgctttgaca atcccctggt gttttgcgaa
    52861 aacattcgag gaagaaaaaa aacagtattc ttatatgcgc ataaccatgt atgtaaatac
    52921 catgtttacc gtgctagtga aatctacgta tggcgtggac agacgccatt cgtgatgtcg
    52981 atagctgcca caaggcaacg gtcttctcac cgtagaccca ggcattgcgc gccgtgaatc
    53041 ttcatgattt cggtctatcg tgacgggtag cgactctgaa cagtgatgtt tcagggtcag
    53101 acaggagtag ggaaggaata cagagagaca ataataatgg tagatagcaa gaagcgccct
    53161 ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg agttgcaaaa ggatgggcgt
    53221 atttctaacg tcgagctttc taaacgtgtg ggactttccc caacgccgtg ccttgagcgt
    53281 gtgcgtcggc tggaaagaca agggtttatt cagggctata cggcgctgct taacccccat
    53341 tatctggatg catcacttct ggtattcgtt gagattactc tgaatcgtgg cgcaccggat
    53401 gtgtttgaac aattcaatac cgctgtacaa aaacttgaag aaattcagga gtgtcattta
    53461 gtatctggtg atttcgacta cctgttgaaa acacgcgtgc cggatatgtc agcttaccgt
    53521 aagttgctgg gggaaaccct gctgcgtctg cctggcgtca atgacacacg gacatacgtt
    53581 gttatggaag aagtcaagca gagtaatcgt ctggttatta agacgcgcta acacggaaca
    53641 ggtgcaaaat cggcgtattt tgattacact cctgttaatc catacagcaa cagtactggg
    53701 gtaacctggt actgttgtcc gttttagcat cgggcaggaa aagcctgtaa cctggagagc
    53761 ctttcttgag ccaggaatac actgaagaca aagaagtcac attgacaaag ttaagtagcg
    53821 gccgccgcct tctggaggcg ttgctgatcc ttattgtcct gtttgccgtc tggttgatgg
    53881 ctgccttact aagctttaac ccttcggacc ccagctggtc gcaaacggcc tggcatgaac
    53941 ctatccataa tttaggtggg atgcccggtg cgtggttggc agatacgctg ttctttattt
    54001 ttggcgtgat ggcttacacc attcccgtca ttattgtcgg cggttgttgg tttgcctggc
    54061 gtcatcagtc cagcgacgaa tacattgatt attttgccgt ttcgctacgc atcattggcg
    54121 ttttggcgct catccttacc tcctgtggtc tggcggcaat caacgctgac gatatctggt
    54181 attttgcctc cggtggcgtc attggcagct tactaagcac tacgctacaa ccactgctac
    54241 acagtagcgg gggaactatt gcgctgctct gcgtttgggc agcgggcctg acgttgttca
    54301 ccggttggtc atgggtgacc attgctgaaa aactcggcgg ctggatttta aacattctca
    54361 ccttcgccag taatcgtacc cgtcgcgatg atacctgggt cgatgaagat gagtatgaag
    54421 acgacgaaga gtatgaagat gaaaatcacg gcaaacagca tgaatcacgc cgtgcccgta
    54481 ttcttcgcgg cgcgctagcg cgtcgtaaac ggttggcgga aaaattcatt aatccgatgg
    54541 ggcggcaaac agacgctgcg ttgttctccg gtaagcggat ggatgatgac gaagagatta
    54601 cctacactgc acgcggtgtg gctgccgacc cggacgacgt cctatttttg ggcaatcgtg
    54661 caacgcagcc agaatatgac gaatacgatc cattattaaa cggtgcgcca attaccgaac
    54721 ctgtcgctgt agcagctgct gctaccacgg cgacacaaag ctgggctgcg ccggttgaac
    54781 ctgtgactca gacgccgcct gttgcctctg ttgatgttcc accttcgcaa cctacagtag
    54841 cctggcagcc tgtaccgggt ccacaaacgg gagagccggt tattgctcct gcaccggaag
    54901 gttacccaca gcagtcacaa tatgcgcagc ctgcagtgca atataatgag ccgctgcaac
    54961 aaccagtaca gccgcagcag ccgtattatg cacctgcagc tgaacaacct gcgcaacaac
    55021 cttactacgc tcctgcggcg gaacaacctg ttcagcaacc gtattacgca actgcgccgg
    55081 aacagcctgc gcaacagccg tattatgccc ctgcgccaga acaaccggtg gcaggtaacg
    55141 cctggcaagc cgaagagcag caatccattt ttgctccaca gtctacatac cagactgagc
    55201 aaacttatca gcagccagcc gctcaggagc cgttgtacca acagccgcaa cccgttgaac
    55261 agcagcctgt tgtggagcct gaacccgttg tagaagagac aaaacccgcg cgtccgccgc
    55321 tttactattt tgaagaagtg gaagagaagc gagcccgtga acgtgaacaa cttgcggcct
    55381 ggtatcaacc gattccagaa ccggttaaag aaccagaacc gatcaaatct tcgctgaaag
    55441 caccttctgt tgcagcagta cctccagtag aagccgctgc cgctgtttct ccgctggcat
    55501 ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc aaccgttgcc gcgccagtct
    55561 tcagtctggc aaatagcggt ggaccgcgtc cgcaggtcaa agaggggatt ggcccgcagt
    55621 tgccacgacc gaaacgtatc cgcgtgccaa ctcgtcgtga attggcgtct tacggtatta
    55681 agctgccctc acagcgtgcg gcggaagaaa aagcccgtga agcccggcgc aatcagtatg
    55741 attctggcga tcagtataac gatgatgaaa tcgatgcgat gcagcaggat gaactggcac
    55801 gtcagttcgc ccagacacag cagcaacgct atggcgaaca gtatcaacat gatgtgcccg
    55861 taaacgcaga agatgcagat gctgcggcag aggctgaact ggctcgtcag tttacgcaaa
    55921 ctcaacaaca acgttattcc ggcgaacaac cggctggggc gaatccgttc tcgctggatg
    55981 attttgaatt ttcgccaatg aaagcgttgc tggatgatgg tccacacgaa ccgctgttta
    56041 cgccaattgt tgaacctgta cagcagccgc aacaaccggt tgcaccacag cagcagtatc
    56101 agcagccaca acagccggtt gcgccacagc cgcagtatca gcagccacaa cagcaggttg
    56161 cgccgcagcc acaatatcag cagccgcaac aaccggttgc gccacagcag caatatcagc
    56221 agccgcaaca accagttgcg ccgcagccgc agtatcaaca gccgcaacaa ccggttgcac
    56281 cgcagccgca gtatcagcag ccacaacagc cagttgcgcc acagccgcag gataccctgc
    56341 ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt gcacaaaccg acgacgccgc
    56401 tgccttctct ggatttgctg acaccgccgc cgagcgaagt ggagccggta gatacctttg
    56461 cgcttgaaca aatggcgcgt ctggtggaag cgcgtctggc tgatttccgt attaaagccg
    56521 atgtcgttaa ttactctccg gggccagtta tcactcgctt tgaacttaac ctggctcctg
    56581 gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct tgcccgttca ctttcgacgg
    56641 tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta tgtaggtctg gagttaccaa
    56701 ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga taacgccaaa ttccgcgata
    56761 atccgtcgcc attaaccgtg gtgctgggta aagatatcgc cggtgagccg gtggttgccg
    56821 atctggcgaa aatgccgcac ttgttggttg cggggactac cggttccggt aaatctgtcg
    56881 gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat gtgcgtttca
    56941 tcatgatcga cccgaaaatg ctggagcttt cggtttatga aggcattccg cacctgttaa
    57001 cggaagtcgt tactgatatg aaagatgccg ccaacgcgct gcgctggtgt gttaacgaga
    57061 tggagcgtcg gtataaactg atgtctgcgc tgggtgtgcg taatctggcg ggttataacg
    57121 aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc agacccgtac tggaagccgg
    57181 gtgacagtat ggatgcccag catccggtgc tgaaaaaaga accatacatt gtcgtgttgg
    57241 ttgacgaatt tgccgacctg atgatgacgg taggtaaaaa agtggaagag ctgatagcac
    57301 gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaact cagcgtccat
    57361 cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc gcctttaccg
    57421 tatccagtaa gattgactca cgtaccattc ttgatcaggc tggcgcggaa tcactgctgg
    57481 gtatggggga tatgctctac tctgggccga actccacgtt gccggtacgt gtccatggtg
    57541 cttttgttcg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc
    57601 cacagtatgt tgatggcatc acctccgaca gcgaaagcga aggtggtgcg ggtggtttcg
    57661 atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac
    57721 gcaaagcgtc aatttctggc gtacagcgtc agttccgcat tggttataac cgtgcagcgc
    57781 gtattatcga acagatggaa gcgcagggga ttgtcagcga acaggggcac aacggtaatc
    57841 gtgaagtgct ggccccaccg ccgtttgact aattaatgca ttgccggata aggcgcggta
    57901 gcgtcgcatc cggcactcta tcaactgaaa attcagtatt ttcttctttc ctcaagctga
    57961 ttattagcct ggaatagaga gtagagggaa ctcccgatcg ggagtgacgt aatttgagga
    58021 ataatgatga aaaaaattgc tatcacctgt gcattactct caagcttagt agcaagcagc
    58081 gtttgggctg atgccgcaag cgatctgaaa agccgcctgg ataaagtcag cagcttccac
    58141 gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga aggtcagggc
    58201 gatctgtggg tgaaacgtcc aaacttattc aactggcata tgacacaacc tgatgaaagc
    58261 attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga gcaagctacg
    58321 gcaacctggc tgaaagatgc caccggtaat acgccgttta tgctgattgc ccgcaaccag
    58381 tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt cctgacgccg
    58441 aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga tggcacaatc
    58501 catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcagct gaaatcccag
    58561 caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg cgtcacggta
    58621 gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt tttcggataa
    58681 tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt atatcggtca
    58741 gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct atcgaagccg ggcatttgca
    58801 ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg aagtgattgc
    58861 ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc acctctggcg tgaaagagat
    58921 tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca ggtcgccgca ctattctttt
    58981 tgttgacgaa gttcaccgtt tcaacaaaag ccagcaggat gcatttctgc cacatattga
    59041 agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg agcttaattc
    59101 ggcactgctt tcccgtgccc gtgtttatct gttgaaatcc ctgagtacag aggatattga
    59161 gcaagtacta actcaggcga tggaagacaa aacccgtggc tatggtggtc aggatattgt
    59221 tctgccagat gatacacgac gtgccattgc agaactggtg aatggcgacg cgcgccgggc
    59281 gttaaatacg ctggaaatga tggcggatat ggccgaagtc tatgatagcg gtaagcgggt
    59341 cctgaagcct gaattactga ccgaaatcgc cggtgaacgt agcgcccgct ttgataacaa
    59401 aggcgatcgc ttttacgatc taatttccgc actgcataag tcggtacgtg gtagcgcacc
    59461 cgatgcggcg ctgtactggt atgcgcgaat tattaccgct ggtggcgatc cgttatatgt
    59521 cgcgcgtcgc tgtctggcga ttgcgtctga agacgtcggt aatgccgatc cacgggcgat
    59581 gcaggtggca attgcggcct gggattgctt tactcgcgtt ggcccggcgg aaggtgaacg
    59641 cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg ctgtctacac
    59701 tgcgtttaaa gccgcgctgg ccgatgctcg cgaacgcccg gattatgacg tgccggttca
    59761 tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg aatatcgtta
    59821 cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc cggaaatagc
    59881 acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg gcgaaaagct
    59941 cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt aatgttatcg
    60001 ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat ctcccattta
    60061 attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg agccagacgc
    60121 agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc tgggcgctct
    60181 tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg agcgtaactc
    60241 ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc ctttacgtct
    60301 ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctag atgctttaca
    60361 ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg cctgcagatg aagtgccggt
    60421 aggtaaagac gaaaatgaca acgttgaagt cagccgctgg ggtaccccgc gtgagtttga
    60481 ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac tctggcctcg actttgcagc
    60541 tgcagttaag ctgactggtt cccgctttgt ggtaatgaaa gggcagattg ctcgcatgca
    60601 ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct acagtgagaa
    60661 ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc tgccgaaatt
    60721 tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca gtaactatgc
    60781 gctgatccca acggcagaag ttccgctgac taacctggtg cgcggtgaaa tcatcgatga
    60841 agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg aagccggttc
    60901 atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag ttgaaatggt
    60961 gcagatcgtg cgcccagaag actcaatggc ggcgctggaa gagatgactg gtcatgcaga
    61021 aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc atcctttgca ctggcgacat
    61081 gggctttggc gcttgcaaaa cttacgacct ggaagtatgg atcccggcac agaacaccta
    61141 ccgtgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta tgcaggcacg
    61201 ttgccgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg gttctggtct
    61261 ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat cagcaggctg atggtcgtat
    61321 tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatattg gctaataccc
    61381 aatttttctg aatctaaaaa gcgcctttag ggcgcttttt ttgtctccct ttgataccga
    61441 acaataatta ctcctcactt acacgtaata ctactttcga gtgaaaatct acctatctct
    61501 ttgattttca aattattcga tgtatacaag cctatatagc gagctgctat agaaataatt
    61561 acacaatacg gtttgttact ggaatcaatc gtgatcaagc ttgagtgagc cattatgaaa
    61621 acgaaaatcc ctgatgcggt attggctgct gaggagagtc gccgtggttt ggtaaaaacg
    61681 acagcgatcg gcggcctggc aatggccagc agcgcattaa cattaccttt tagtcggatt
    61741 gcgcacgctg tcgatagcgc cattccaaca aaatcagacg aaaaggttat ctggagcgcc
    61801 tgtacagtta actgtggtag tcgctgcccg ctacgtatgc acgtcgtgga cggtgaaatc
    61861 aaatatgtcg aaacggacaa taccggcgat gacaattacg acggcctgca ctaggttcgc
    61921 gcctgcctgc gtgggcgttc catgcgtcgc cgtgtctaca atccggaccg cctgaaatat
    61981 ccgatgaaac gagtcggggc gcgcggtgaa ggcaaattcg agcgcattag ctgggaagaa
    62041 gcctacgaca tcatcgcgac caatatgcag cgcctgatca aagagtacgg caacgagtct
    62101 atctatctga actatggcac cggtacgctg ggcggcacca tgacccgctc ctggccgccg
    62161 ggaaataccc tggtcgcgcg cctgatgaac tgctgcggcg gttatcttaa ccattacggt
    62221 gactactctt ccgcgcaaat tgcggaaggt ttgaaccata cctacggcgg ctgggcagat
    62281 ggtaacagcc cgtcggatat cgaaaacagt aaattggtag tgctgtttgg taataaccca
    62341 ggcgaaacgc gaatgagtgg cggtggggtg acttactatc ttgaacaggc acgccagaaa
    62401 tctaatgctc gcataatcat catcgatccg cgctataccg acaccggtgc cgggcgcgaa
    62461 gatgagtgga tccctattcg tccaggaaca gatgccgcac tggttaacgg tctggcgtac
    62521 gtcatgatca ctgaaaacct ggtggatcag gcattcctcg ataaatattg cgttggctat
    62581 gacgagaaaa ccctgcccgc cagtgcgccg aaaaatggtc attataaagc ttatattctg
    62641 ggtgaagggc cagatggcgt ggctaaaaca ccggaatagg cctcgcaaat caccggtgtt
    62701 ccggcagaca aaatcatcaa actggctcgt gaaatcggca gtaccaaacc ggcgtttatc
    62761 agccagggat ggggcccgca gcgtcacgct aacggtgaaa tcgcaacccg tgctatctcg
    62821 atgctggcga ttctgaccgg taacgttggt attaatggag ggaacagcgg cgcgcgtgaa
    62881 ggttcataca gcttaccgtt tgtccgtatg ccgaccttgg aaaacccgat ccagaccagc
    62941 atttcgatgt ttatgtggac cgatgccatt gaacgtggcc cggaaatgac ggcgctgcgt
    63001 gatggtgtgc gcgggaaaga taagctggat gtgccgatca aaatgatctg gaactatgcc
    63061 ggtaactgcc tgattaacca gcattctgaa atcaaccgta cccatgaaat cctccaggat
    63121 gataagaagt gcgagctgat tgtggttatc gactgccaca tgacctcatc ggcgaaatat
    63181 gctgacatcc tgctgcctga ctgcaccgct tccgaacaga tggactttgc actggatgca
    63241 tcctgcggga atatgtctta cgtgattttc aacgatcagg tgattaaacc gcgctttgaa
    63301 tgtaagacca tctatgaaat gaccagcgaa ctggcaaaac gtcttggcgt tgagcaacag
    63361 tttactgaag gccgtaccca ggaagagtgg atgcggcatc tgtatgccca gtcgcgggaa
    63421 gcgattcctg aactgccaac gtttgaagag ttccgcaagc aggggatctt taaaaagcgc
    63481 gacccacaag ggcatcacgt tgcttataaa gccttccgtg aagatccgca ggcaaaccca
    63541 ctgactacgc catcgggcaa aattgagatt tattcgcagg cgctggctga cattgccgct
    63601 acctgggaat tgccagaagg cgatgtgatc gatccactgc cgatctacac gccgggcttt
    63661 gaaagttatc aggatccgct gaacaaacag tatccgctgc agcttacagg tttccactat
    63721 aaatctcgcg ttcactcaac ttacggcaac gttgatgtgc tgaaagcggc ttgccgtcag
    63781 gaaatgtgga tcaacccgct tgatgcccaa aaacgcggta tccacaacgg cgataaagtc
    63841 aggatcttta acgatcgtgg tgaggttcat attgaggcga aagtgacgcc acgaatgatg
    63901 ccgggcgtgg tcgcactggg tgaaggcgcc tggtatgacc cggatgcaaa acgtgtcgat
    63961 aagggcggtt gtatcaacgt actgaccact caacgtccgt ctcctctcgc taaggggaat
    64021 ccgtcacata caaaccttgt tcaggttgaa aaggtgtaag gagtaaccga tgacaaccca
    64081 gtatggattt tttattgatt ccagccgttg caccggttgc aaaacctgcg agctggcttg
    64141 taaagactac aaagatttga cgccagaagt cagtttccgc cgcatttatg aatatgctgg
    64201 cggcgactgg caggaagata acggtgtctg gcaccagaac gtgtttgcct actatctgtc
    64261 gatttcgtgc aaccactgcg aagatccggc ttgtactaaa gtctgcccga gcggtgcgat
    64321 gcataaacgc gaagatggtt ttgttgtggt cgatgaagat gtatgcattg gctgccatta
    64381 ctgtcatatg gcttgcccgt atggcgcacc gcaatataac gaaacgaaag gccatatgac
    64441 caaatgcgat ggttgttatg accgtgttgc ggagggtaaa aagccgatct gtgttgaatc
    64501 ttgcccgctg cgggcgctgg atttcggccc tatcgacgag ctgcgtaaaa aacatggcga
    64561 tctggcggcc gttgcgccgt tgccgcgagc tcactttacc aaaccgaata ttgtgatcaa
    64621 acccaatgcc aatagccgcc cgacagggga taccactggc tatctggcaa acccgaagga
    64681 ggtgtgagat gggaagtgga tggcatgaat ggccgctgat gatcttcacg gtcttcgggc
    64741 aatgtgtggc gggtggtttt atcgttctgg ctttggcgct gctcaaaggc gacctgcgag
    64801 cagaagccca gcagcgtgtt atcgcctgca tgtttggctt atgggtactg atgggcattg
    64861 gctttatcgc ctctatgctc catcttggtt caccgatgcg cgcctttaac tcgctcaacc
    64921 gggtaggggc ttcagcactc agtaacgaaa tcgccagcgg ttcgatcttt tttgctgtag
    64981 gcggcatcgg ctggttgctg gcaatgctga aaaagctgtc accggcattg cgtacgctat
    65041 ggctggtggt gacgatggtt cttggcgtcg tctttgtctg gatgatggtg cgtgtgtata
    65101 acagcattga taccgttccg acctggtaca gcatctggac gccgatgggc ttcttcctga
    65161 cgatgttcat gggcggcccg ttactgggtt acctgttgct gagtctggct ggcgtcgatg
    65221 gctgggcgat gcgtctgctg ccagccattt ctgtactggc actggtggtg agtggcgtga
    65281 tgtcagtgat gcagggcaca gagctggcaa ctattcatag ttctgtgcag caggccgcag
    65341 cgctggtgcc ggactatggt gcgctgatgt cctggcggat cgtgcttttg gccgttgccc
    65401 tgtgcttgtg gattgcacca cagctaaaag gttatcagcc tgcggttccg ctactatccg
    65461 tctcattcat tctgcttctg gcaggggaat taatcggtcg cggcgtattc tatggtttgc
    65521 atatgaccgt ggggatggcc gtcgcaagct aatcagtaac aaccggggtt tcggccccgg
    65581 ttctctttta tttctgcttc gttaacgtgt cataactggt catcaaatta cgataatcag
    65641 gaatatggtt agagaacaac gtcgccagtc cttcaatatc attacgccag tcacggtgca
    65701 gctcgcaagc cacgccgaac catgtcatca attgcgcacc agcttgcgac atgcgatccc
    65761 acgccgaatg ccgggtaatt tcattaaagg tccctgatgc atcagtcacg acaaagacat
    65821 caaacccttc ttcaatggct gaaagcgccg ggaatgccac gcaaacttcg gttaccacac
    65881 cggcaataat taactgtttt ttacctgtcg ctttgactgc ttttacaaaa tcttcgttat
    65941 cccaggcgtt aatattgccc gggcgagcaa tgtaaggtgc atcggggaat tgtgctttca
    66001 gttctagaac taatgggccg ttaggaccgg tttcaaaact ggtggtaaga atagttggta
    66061 agttgaaata cttagccagg tcacccagcg ccagcacgtt atttttaaac ttatcgggtt
    66121 cgatatcccg tacaagggaa agtaaaccag cctggtgatc aacaagcaaa acggcagcat
    66181 catttttatc aagacgaaca tacggtttgg tcatcctctt ctcctttcga gagtcagtgg
    66241 tacgtctgaa atcggtcaga cgcgatgcat tgctctgaaa gcatagacgg gaaatatgag
    66301 tttgctgtga ccatgaaatt tttcgactga acgaagcgta tgaaatttgt gttagatcaa
    66361 taaaaacaat cagatgagat taatcatctc ttcagcatta gcgttatgaa tgagtaatat
    66421 aaatcgataa taccgccgct gcgatatgcg cgtattttca cccattgaca atgtttttgg
    66481 cggtggcatg atgcgcatga aatttgaact tcctcacggt tttaattcat gtccacgtat
    66541 acccggcctg tcatgctttt actgtctggc ctgcttttgt tgactctggc gatcgcggtg
    66601 ttaaatacac tcgtgccgct ttggctcgcc caggaacaca tgtccacatg gcaggtaggc
    66661 gttgtcagct catcctattt taccggcaac cttgtcggta cattgctgac agggtatgtc
    66721 attaaacgca ttggctttaa ccgcagctat tatctgtcct ccttcatttt tgctgctggc
    66781 tgtgccggcc ttggcctgat gattggtttc tggagctggt tggcgtggcg ttttgtcgcg
    66841 ggcgtcggct gtgccatgat ttgggtggtt gttgagagcg cgctgatgtg cagtgggacg
    66901 tcacgtaacc gtgggcgttt gcttgctgcg tatatgatgg tttattacgt gggaacgttt
    66961 ttaggccagt tactggtcag caaagtctca accgagctga tgagcgtatt gccgtgggtt
    67021 acaggtttga cattggcagg gatcttaccg ctgttgttta cgcgtgtcct gaatcagcag
    67081 gctgaaaacc atgattcgac gtcaattacg gcaatgctaa aactccgtca ggcgcggctt
    67141 ggcgtgaatg gctgcattat ctcaggaatc gttctgggat ctctatatgg cctgatgcct
    67201 ctgtacctca atcacaaagg ggtgagcaat gccagtattg gtttctggat ggcggtactg
    67261 gtcagtgcgg gtatcctcgg acaatggccg attggacgtc tggcggataa gtttggtcga
    67321 ctgctggttt tgcgtgttca ggtctttgtc gtcattctcg gcagtattgc gatgcttagc
    67381 caggcggcga tggccccagc gttattcatc ctcggtgccg ctggctttac gctatatccg
    67441 gtggcgatgg cctgggcttg tgagaaagtt gaacatc
//
LOCUS       DAJTNM010000007        66599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTNM010000007 DAJTNM010000000
VERSION     DAJTNM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.007"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO172_000550"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO172_000550"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5510848.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO172_000551"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO172_000551"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5510849.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO172_000552"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO172_000552"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5510850.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO172_000553"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO172_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5510851.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO172_000554"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO172_000554"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5510852.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDASHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO172_000555"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO172_000555"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5510853.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO172_000556"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO172_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5510854.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO172_000557"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO172_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5510855.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMDICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLN"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO172_000558"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO172_000558"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5510856.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO172_000559"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO172_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5510857.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO172_000560"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO172_000560"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5510858.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO172_000561"
     CDS             complement(15665..16702)
                     /locus_tag="OO172_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5510859.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO172_000562"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO172_000562"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5510860.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19304)
                     /gene="ggt"
                     /locus_tag="OO172_000563"
                     /pseudo
     CDS             complement(17582..19304)
                     /gene="ggt"
                     /locus_tag="OO172_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            19424..19864
                     /locus_tag="OO172_000564"
     CDS             19424..19864
                     /locus_tag="OO172_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5510861.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="OO172_000565"
     CDS             complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="OO172_000565"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5510862.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20591..21581)
                     /locus_tag="OO172_000566"
                     /pseudo
     CDS             complement(20591..21581)
                     /locus_tag="OO172_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="OO172_000567"
     CDS             complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="OO172_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5510863.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="OO172_000568"
     CDS             complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="OO172_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5510864.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="OO172_000569"
     CDS             complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="OO172_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5510865.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25123..25836)
                     /gene="livF"
                     /locus_tag="OO172_000570"
     CDS             complement(25123..25836)
                     /gene="livF"
                     /locus_tag="OO172_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5510866.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSSYLGG"
     gene            complement(25838..26605)
                     /gene="livG"
                     /locus_tag="OO172_000571"
     CDS             complement(25838..26605)
                     /gene="livG"
                     /locus_tag="OO172_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5510867.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26602..27879)
                     /gene="livM"
                     /locus_tag="OO172_000572"
     CDS             complement(26602..27879)
                     /gene="livM"
                     /locus_tag="OO172_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5510868.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27876..28802)
                     /gene="livH"
                     /locus_tag="OO172_000573"
     CDS             complement(27876..28802)
                     /gene="livH"
                     /locus_tag="OO172_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5510869.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28850..29959)
                     /gene="livK"
                     /locus_tag="OO172_000574"
     CDS             complement(28850..29959)
                     /gene="livK"
                     /locus_tag="OO172_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5510870.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30383..30766
                     /gene="panM"
                     /locus_tag="OO172_000575"
     CDS             30383..30766
                     /gene="panM"
                     /locus_tag="OO172_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5510871.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30965..32068)
                     /gene="livJ"
                     /locus_tag="OO172_000576"
     CDS             complement(30965..32068)
                     /gene="livJ"
                     /locus_tag="OO172_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5510872.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="OO172_000577"
     CDS             complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="OO172_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5510873.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="OO172_000578"
     CDS             complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="OO172_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5510874.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="OO172_000579"
     CDS             complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="OO172_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5510875.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="OO172_000580"
     CDS             complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="OO172_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5510876.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36806..37402
                     /gene="rsmD"
                     /locus_tag="OO172_000581"
     CDS             36806..37402
                     /gene="rsmD"
                     /locus_tag="OO172_000581"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5510877.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37392..37661
                     /locus_tag="OO172_000582"
     CDS             37392..37661
                     /locus_tag="OO172_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5510878.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37664..38023)
                     /locus_tag="OO172_000583"
     CDS             complement(37664..38023)
                     /locus_tag="OO172_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5510879.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38164..38790
                     /locus_tag="OO172_000584"
     CDS             38164..38790
                     /locus_tag="OO172_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5510880.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38864..41062
                     /gene="zntA"
                     /locus_tag="OO172_000585"
     CDS             38864..41062
                     /gene="zntA"
                     /locus_tag="OO172_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5510881.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="OO172_000586"
     CDS             complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="OO172_000586"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5510882.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41630..42295
                     /locus_tag="OO172_000587"
     CDS             41630..42295
                     /locus_tag="OO172_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5510883.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42368..42925
                     /gene="dcrB"
                     /locus_tag="OO172_000588"
     CDS             42368..42925
                     /gene="dcrB"
                     /locus_tag="OO172_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5510884.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42929..44146)
                     /locus_tag="OO172_000589"
                     /pseudo
     CDS             complement(42929..44146)
                     /locus_tag="OO172_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44278..45327
                     /locus_tag="OO172_000590"
     CDS             44278..45327
                     /locus_tag="OO172_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5510885.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45382..45969
                     /gene="acpT"
                     /locus_tag="OO172_000591"
     CDS             45382..45969
                     /gene="acpT"
                     /locus_tag="OO172_000591"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5510886.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46080..47654
                     /gene="nikA"
                     /locus_tag="OO172_000592"
     CDS             46080..47654
                     /gene="nikA"
                     /locus_tag="OO172_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5510887.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47654..48598
                     /gene="nikB"
                     /locus_tag="OO172_000593"
     CDS             47654..48598
                     /gene="nikB"
                     /locus_tag="OO172_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5510888.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48595..49428
                     /gene="nikC"
                     /locus_tag="OO172_000594"
     CDS             48595..49428
                     /gene="nikC"
                     /locus_tag="OO172_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5510889.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLILGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49428..50192
                     /gene="nikD"
                     /locus_tag="OO172_000595"
     CDS             49428..50192
                     /gene="nikD"
                     /locus_tag="OO172_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5510890.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50189..50995
                     /gene="nikE"
                     /locus_tag="OO172_000596"
     CDS             50189..50995
                     /gene="nikE"
                     /locus_tag="OO172_000596"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5510891.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51001..51402
                     /gene="nikR"
                     /locus_tag="OO172_000597"
     CDS             51001..51402
                     /gene="nikR"
                     /locus_tag="OO172_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5510892.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51522..51881)
                     /locus_tag="OO172_000598"
     CDS             complement(51522..51881)
                     /locus_tag="OO172_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5510893.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51878..52153)
                     /locus_tag="OO172_000599"
     CDS             complement(51878..52153)
                     /locus_tag="OO172_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5510894.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52212..53336)
                     /locus_tag="OO172_000600"
     CDS             complement(52212..53336)
                     /locus_tag="OO172_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5510895.1"
                     /translation="MRQLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="OO172_000601"
     CDS             complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="OO172_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5510896.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56068..57135)
                     /locus_tag="OO172_000602"
     CDS             complement(56068..57135)
                     /locus_tag="OO172_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5510897.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57501..59123)
                     /locus_tag="OO172_000603"
     CDS             complement(57501..59123)
                     /locus_tag="OO172_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5510898.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINKNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59385..60974)
                     /locus_tag="OO172_000604"
     CDS             complement(59385..60974)
                     /locus_tag="OO172_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5510899.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEESQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61358..62389
                     /locus_tag="OO172_000605"
                     /pseudo
     CDS             61358..62389
                     /locus_tag="OO172_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62923..63687)
                     /locus_tag="OO172_000606"
     CDS             complement(62923..63687)
                     /locus_tag="OO172_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5510900.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSANLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNGETYAICSEVLLYD"
     gene            63934..64695
                     /locus_tag="OO172_000607"
     CDS             63934..64695
                     /locus_tag="OO172_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5510901.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSLQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64802..65302)
                     /locus_tag="OO172_000608"
     CDS             complement(64802..65302)
                     /locus_tag="OO172_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510902.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65453..>66599)
                     /locus_tag="OO172_000609"
     CDS             complement(65453..>66599)
                     /locus_tag="OO172_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5510903.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDQK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16175 a        17873 c        16342 g        16209 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgtaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaagtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgccgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgagg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta attcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagtag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatgtcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgaga ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cgcccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgccgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc taaaacaact tcctgagagc
    19261 agagcagcaa tggccacccg gcgtaaaaac gtcggtttta tcatcgttat tctccagaga
    19321 ttaaggggca accccagcta agcctggtat ataactctga attaatcatc gttttgtcgg
    19381 gaagcgagta aacttaaagg atatcttcag aggaggatac gcgatgaaac gacttctgat
    19441 tcttacggca ctcctgccgt ttgtcggctt tgcacagccc attaatactc tgaacaaccc
    19501 taaccagccg gggtatcaga tacccagcca gcagcggatg caaacccaga tgcagactca
    19561 gcaaatccag caaaaaggga tgctgaatca gcaactgaaa acgcaaactc agttgcaaca
    19621 gcagcattta gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt cacagccggg
    19681 ggagcgaaat cccgcccggc agcaaatgct gcccaacacc aacggcggga tgttaaacag
    19741 caaccgtaat ccggatagtt cgttgaatca gcagcatatg ctgccggaga ggagaaacgg
    19801 cgacatgctg aatcagccca gcacgccgca gcctgacatt ccgttgaaaa ctattgggcc
    19861 gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga tgcaatccac gccccagcgc
    19921 agcaactctg ccgcgcgctg gggtttattg acggtataaa ccagaatccg taatccggcg
    19981 tctttcaact gcatcactcg cgctttatcg agtaacttat gattgagatg aatagagacg
    20041 cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag caataaacca
    20101 cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag catcaatctc aaacgatgac
    20161 agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac cattttgccc
    20221 gttaatggtc cagtgccagt ggtgggtttg atttcgatat tcgccatcat cccgtgttcg
    20281 cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct cacctttaaa cgctttgctg
    20341 taccaactgc ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc cgcgacgccc
    20401 cagccgttgc tggtgcgttc gagattgtcg tcatggagca ggaagatctc gccatctttc
    20461 gataacttcg cgtcaaattc gatcatctta tgaccgtatt ttgccccgac gtcgattgcc
    20521 gccagggtgt tttccggggc cagcttaccg ccgccacgat gagcgacgat gcggggataa
    20581 ggccagttac tcatactcgt tgtcctgttt caccatcaaa aagatgcagc tgattttccg
    20641 ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc agtcgcacca
    20701 ccagcttctg cttgcgagct tagcgcaata tgttccgggc gaatgccgag ggtcatttta
    20761 cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc gtccagttcg
    20821 aaatgcgtgc cttcattatt catgcggcct gtcagcaggt tcatcgccgg gctgccgata
    20881 aaactcgcca caaacaggct ggcgggcttt tcgtagactt ccaccggcgt gccaatctgt
    20941 tcggcaacgc cgccgttcat caccattact cgctgggcga gcgtcatcgc ctcaacctga
    21001 tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcagctgttg cagttcaaga
    21061 cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg gctcatcaaa caagaacacc
    21121 gccggatcac gcacaatcgc gcgtcccatc gccacacgct ggcgctgacc gccggaaagc
    21181 tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa tgcgcgccgc ttctttaacg
    21241 cgctcggcta tttgctgctt gcccatgccg cgaattttca gcccccacgc catgttttct
    21301 tcgacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat cccgcgatct
    21361 ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc ggtcaccctc
    21421 tccagcccgg caaccatacg cagcagcgtc gatttcccgc agccagacgg cccgaccatc
    21481 acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgaccta ggttttgcca
    21541 tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct cactatcgac
    21601 caggccgcgc acgaaggcac gctgcatcac taaaacaatc actactggtg ggatcagggt
    21661 taacaacatc gccgccatca ctgagttcca ttccgtggtg ccttcgccag tggcgatcat
    21721 ccctttgatc cctgccacgg tggtgccgag atccacgtcg gtaataatca acaacggcca
    21781 caaatactgg ttccagccgt aaataaaggt gatcacaaac agcgccgcca gattagtttt
    21841 ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt gacgcaccgt cgatccgcgc
    21901 cgcttccacc agttcatccg gcagcgtcat aaagaactgg cgaaacagaa acgtggcggt
    21961 cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcatct tcaggttggc
    22021 gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg tgataaaaat
    22081 catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa atgccgagag
    22141 catcgagaca gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc tgttaagcaa
    22201 catccgccag aacggcgcgc tatttgtgcc taccccgttc acccagatgt tgtggatgtt
    22261 ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg gcgtagacgg cctgtttatc
    22321 cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga tcccgaggat
    22381 cagcatggta tggctgaata tcgtcagcca cggacggttc tcaattattg gtaacgcacc
    22441 ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa catcaacacc
    22501 accgactgtg cggcagacga agccagatcc agtccggtaa aaccttcgcg gtagatctta
    22561 taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg tggcggcgtc gatcaccggg
    22621 aaggtgtcga agaaggcata caccaggttc actaccagca ggaagaaact caccggggcg
    22681 ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc ctgcaccgtc gatggctgcg
    22741 gcttcgatca acgaacgggg aatggattgc agcgcggcat agaagaacag aaagttgtag
    22801 ctgatttgct tccacaccga ggcaaacacc accagaaaca tcgcctgacc gctattttgc
    22861 gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc gcgaccgggg
    22921 ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata cggcagcaac
    22981 attaaggttt ggtagaaacg actgccgcgc acgatgtact ccaccagcgc cgcaaagaac
    23041 agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt cgtccagaag
    23101 gagtcgagat aatagctgtc atgaaacagc gtgacgaagt tatccagacc gacaaactgg
    23161 ctggagaaac caaacggatc gacgctttgt agcgagtacc acaacgcttc gcccgcaggc
    23221 cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata aggcagccag
    23281 cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac tgtgccggat
    23341 gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac gcatccggca
    23401 ttacactgat taagacttcg tcgatttctc aaagcggcgc agtaactggt tcccacgctc
    23461 aactgcggta tccagcgcct gctgtggcgt cttcttaccg gtccacacgc tctcaagctc
    23521 ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgaagccctt tggtgaacgg
    23581 caacggcggc ttgttcagca tctgacgcgt cgcggtatcc gcacctgggt ttttctcgta
    23641 aaagccctgc tcacgggtca ggtcatacgc cgctttggta attggcagat aaccggtttt
    23701 ctgatgccac tcagcagcgt tttctggctt cgcgaggaag tcgagaaact tcgccacgcc
    23761 ggtatacgtt tctttatctt tgccctgcat cacccacagg ctggctccgc cgataatggc
    23821 gttttgtggc gcatctttcg catcggcgtc gtaaggcatc atacctacgc cgtagttaaa
    23881 tttggcgtac tcgcgaatgt tggcaagaga accggaagag gcggtggtca tcgcgcaatc
    23941 accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga agtcgccctt
    24001 cttgttcatc tcctcgagca tggcgatgtg tttcacctgc tccggcttgt tgaactccag
    24061 caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac cgttccaggc
    24121 gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc acttaatacc
    24181 ggaggctttc agttttgcgg aatagtccgc cagatcctgc caggttttcg gcggctgttc
    24241 cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg gagtcgagct
    24301 gttgaacggc tgggagagta agtggccggt tttgctgtcg gagtagtaac cggaaaccgt
    24361 cggcacaaac tgcgactcat cgaactgaat ccctgcctct ttaaacacgt catacaccgg
    24421 tttaatggct ttcgacgcca tcatggtggc ggtgccaact tcataaacct gcaaaatagc
    24481 cggagcgttg ccggtacgaa atgcggcaat ccccgcgctt aaattctgtt cgtagttgcc
    24541 tttataggtc ggtacaattt tgtaatccgg attttcggcg ttaaaacgtt gggccagaga
    24601 atccacctct ttacccagtt ccccttccat agaatgccag aacggaatgg tcgtcactgc
    24661 ctgtgcattc cccattaacg ccagtccgag cgccagtgct gaagctgtat aatgtaacgg
    24721 tttcatcgtt tatctctctt gttgtaccga atgcgcgaat tcacgcgttt tatgctcgcg
    24781 gggtaacatg acatgctcga attacagaaa aataactttt ttgttacatt tgtaagatag
    24841 taaggtgtca gaaagatgac aaggcggtga cggcgtgggt gagggaaaat gggagatggg
    24901 gcacggataa gcggaaaaat agaaggtctg aatcaaactc tacagattac tcatcgtttc
    24961 atgccggatg cggcgtaaac gccttatccg gcctacaaga tcgtgcaaat tcaatatatt
    25021 gcaactcacc cagtaggcct gataagcgca gcgcatcagg caattttgca tttgtcacct
    25081 gtctcaaagg agtcttttga ctccttatca atcaacgtgt tattacccgc ctaaatacga
    25141 acttctcact gcttcgttcg ccagcagcgc gtcgccggta tcggagagca ccacatggcc
    25201 gttttccagc acgtagccgc gatccgccag ctttagcgcc tggttggcgt tctgctcgac
    25261 gaggaagata gtcatcccct gctcgcgcag ctgctcgatg gtgtcgaaaa tttgctggat
    25321 aataatcggc gcaagaccga gcgatggctc atcaagtaac aacaaacgcg gattgctcat
    25381 cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg gacatggtgc ccgcccgctg
    25441 aacacggcgc tcatgcagac gtggaaacag ttcatacacc cactttatgc gctcctgaaa
    25501 ctggtcacgt tcggcaaaga agccgcccat cgccaggttc tcttccaccg tcatccgcga
    25561 gaagacgcga cgcccttccg ggacaatcgc taccgcttcg cgcatgattt tcgccgtctg
    25621 ccagtcggta atgtctttat catcaaacac aattcgcccg ctggtggcgc gcggatcgcc
    25681 gcataacgtg ccgagcaagg tggttttccc cgccccgttc gcgccaatga gagtgacaat
    25741 ctcgccctga ttgatatgca ggctcacctc atgcagcgcc tggattttgc cgtagtgggc
    25801 gctgactttg tcaaaggaca acatgacttt ttccatctta tgcctcacct aaataggcac
    25861 ggatcacgtc cgggttatta cggatttgct ccggcgtacc gtttgccagt ggcgtcccct
    25921 gattgaccac ataaattcgg tctgaaatcc ccatcaccag cttcatatcg tgttcaatca
    25981 acaagatagt ggtgttgtga tgattgcgca gttcggcaat cagctcatcc agctctttgg
    26041 tttcttttgg gttaagacct gccgccggtt cgtcgagcat taaaatctcc ggctgcgtca
    26101 ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata ggccaggtta cttgcctgac
    26161 ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt ggcggcgcgg tcgagcgctt
    26221 cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc agagaacagc ccggttttca
    26281 gttgctgatg ctgcgccacc agcaggtttt caattaccgt catttcacgg aacagacgca
    26341 catgctggaa ggtgcgcacc acgcccatgc gagcaatttg ctgccccggt aaaccttcaa
    26401 gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt gtagaaaccg gtcaggcagt
    26461 taaaaaccgt ggtttttccg gcaccgttag ggccgattaa cgagacgatt tcctgcgggt
    26521 acagttcaag attgacgttg ttcaccgcca gcaggccgcc gaagcgcatc atcagaccgt
    26581 taacagataa taatggctga ctcatgcctg ctctcctttc gctgcgccgt ttttcagctt
    26641 cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc cagatcatca tcagcaccat
    26701 caaaccaccg agcattaaca tgctgtattc gttgaaatca cgcatcaatt cgcgcgacac
    26761 caccagcaga acagccgcca gaatcactgc aaattgcgag cccataccgc cgagcaccac
    26821 tatcgccagc acaaaggccg attcagcaaa ggtgaaggat tccgggctga caaagccctg
    26881 acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg gcgctgatgg taaaagcggt
    26941 cagcttgata cggcgcgggc ttaagcccag cgaacgacag gcgatctcgt cttcacgcaa
    27001 cgcttcccac gcgcggccca gcggcatccg cagcaggcgg ttaatgacaa acaggcttaa
    27061 caccaccagc agcaacgcca ccaggtagag gaagataacg cgatcggacg gatcgtattt
    27121 caggccaaag aaattactga acgtgtccca gccgccttca cgagcggtac ggctgaactc
    27181 gagtccgaag agtgtcggtt tcgggatctg actgattccg ttcgggccgc cggtgatttc
    27241 agtgttattg agcagcaata tgcgcacaat ttcgccgaaa ccaagggtaa cgatcgccag
    27301 atagtcaccg cgcagacgca gcaccggaaa accgagcagg aaacccgccg ccgctgccat
    27361 cagtccggca atcggcaaac aggtccagaa ccccagaccg taatagtgat tgagcagcgc
    27421 aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc agcaccagca gaccagaaag
    27481 accaacaacc acattcagcc cgagaccgag gataatgtag atcatggtca gagtggcgat
    27541 atccaccgtc ccacgtgaca ccataaacgg ccacgccacc acaagcacca gcagcgccac
    27601 gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg ggcagaataa acttcggccc
    27661 ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa agctggaaga aaaagaccac
    27721 cgccgtgccg ataaacatcc actgccaacg gatgtctgaa gccgtgtcga ccaccagttt
    27781 ggtgccatcc agctccagtt gtacgcccat aaagacgccc gccaggacaa agaacatcgc
    27841 ggcagagagc agtgccattg caatatgcat cggtttcata ctttctctac ctccgggcga
    27901 cccagaatac cggtcggcat caccagcagc accagaatca gcagggcgaa tgagaccaca
    27961 tctttatatt ccgtactcag ataggcagaa gagagcgcct ccgcaatccc taggatcagc
    28021 ccgccaatca tcgcccctgg aatgctgcca atcccaccga gcaccgcagc ggtaaaggct
    28081 ttcatcccgg ccataaagcc gatatagggg ttaatgacgc agtagaactg accgagcagt
    28141 acgccagcca ctgccgccat cgccgcgcca ataacaaagg tcaaagcaat cacccggtcg
    28201 gtgttgatgc caagcaggct cgccattttc agatcttccg cgcaggcacg acaggcgcgc
    28261 cccatacggg aatagcgaat gaaaatcgtc agcgccagca tggcgaggaa ggtgacaatc
    28321 cagatcaccg cctgcatggt ggtaatagag gcagagaagt tttcgctatg gcccaccacc
    28381 cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg aaccttcggt caggctgacg
    28441 tagttttgca ggaagatgga cataccgatt gcggaaataa gcgcaatcag gcgcttagag
    28501 ttacgcaccg ggcggtaagc tacccgttcg atactccagc cgtaggcgct ggcgatgacg
    28561 attgcgccga cgaatcccgc cgccaccagc agccagccgg tatcaatgcc catcatcatc
    28621 agcgcggcga tgatcataaa tgagacgtag ctgccaatca tataaacctc gccgtgggcg
    28681 aagttgatca tgccgataat gccgtaaacc atggtgtagc cgatggctat cagcgcgtag
    28741 gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga aatacaaaaa ctgctcagac
    28801 ataaggtaac ctttctaaac ccgcccgcat tttacgggcg gtgggatgat cacttggctg
    28861 ccgtggatga accgtcggcg tgccactgga agacaccaaa atcaaatccc ttaagatcgc
    28921 ctttttcatc ccagttcagc ggcccaatca cggtgtttgc accgttagct tttaaatctt
    28981 tcaccagcgc cagcggctca tcgctgccgg tacgctcaag ggcagttgcc agagattgca
    29041 ctgccgcgta agtgatccag acataaggcc cggacggatc tttcttgtct gctttcagcg
    29101 catcaacgat gccctggttt gccggatcct ggtcatagcg ttttggcata gtgaccaaca
    29161 tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc gttaccgaca ccttctggcc
    29221 ccataaactg ggttttcagg ccaacggaac gggcttggcg cagcatctgc cccatttccg
    29281 ggtagtaacc gccgtagtaa acgaagtcga tattttcttt tttcaggcgg gcgatcagcg
    29341 cggagaaatc tttctccccg gcggtaatac cgtcgaagaa gacgacgttg gcgttagccg
    29401 ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc atactgttgt ttgtcgtgaa
    29461 tgatggcgat gcgctggggc ttcaccgtct caagaatgta ttttgccgcc gtaggcccct
    29521 gggaagagtc cagtccggca gtacgcataa tgtgttgata accgcgttgg gtcagctccg
    29581 ggttggtcgc tcccggcgag atcatcagaa taccttcgtc ttcgtagata tccgacgctg
    29641 gctgggtaga ggaagaacac agatgaccaa taacgtattt aatgccgtcg ttaacgattt
    29701 tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc gtcatattcc acgccaacca
    29761 gcttgtcgcc cttaattccc cctttgacat taatgtcttt aatcgcctga cgcgcgccgt
    29821 taaattccat atcgccccac tgggcaatcg ggccggacat cgcgccgaca acggcgactt
    29881 taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc aatcatccct gcgatgatag
    29941 ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg atgttgtgtt gctttgtttt
    30001 tatgtgttaa caaatcagac tgttcttttt ttatactgca ctgtttttgc ctgtctgatt
    30061 aaggggttag cgcagtattt tgtgatgata gcgattaaaa tccctatttt tcagtcgatt
    30121 aagaacagat aatattctga atttattgat agataaacag aaaaaagtgc ctttgtcagc
    30181 ataaaataac ggcacaaagg gcggaataat tcactatcat tcaggggatt atgctggaca
    30241 tttttcattc tctaatgttt taactttgta attattgctg ttaaaaaatt aatcacctgc
    30301 caaaagaaat aaaaaagaga aagcctccga ttaaattatt tcgctacact ggttccactt
    30361 ttgtgattta cacgggttac ccatgaagct gaccatcatt cgattagaaa actttagcga
    30421 ccaggaccgg attgacctgc aaaagatctg gccggagtat tccccttcct ctttacaggt
    30481 tgacgataac caccgtatct acgccgcgcg ttttaacgag cgtctgcttg cggccgtgcg
    30541 ggtaacctta agcggcaccg agggagcact ggattctcta cgcgtgcggg aagtcacccg
    30601 ccgtcgcggt gtggggcaat atctgctgga agaggttttg cgtaacaatc ctggcgtttc
    30661 atgctggtgg atggcggatg caggcgtgga agatcgcggt gtgatgacgg cgtttatgca
    30721 ggcactggga tttacggcac agcagggcgg ctgggagaag cgttaatcgt caagttgatt
    30781 tcaaaagtga tgaccaacga gaaattgcct gatgcgctac gcttatcagg cctataatgt
    30841 gtgctgcaat ttactgattt ctttggttct tgtaggccgg ataaggcgtt tacgccgcat
    30901 ccggcatgaa gcaacgtatt cgatattagc aatttggcgg caacccaaag ttgccgatta
    30961 acaattactt cgcatcggtc gccgtgccgt tggcgtgcca gtcaaatacg ccgaactcaa
    31021 agccctttag atcgcctttc tcatcccagg tcagtggccc catcacggta tccacggagt
    31081 tcgcttttag gtatttggcg atttcagccg gatcgtcaga ctgattcagg cccgcctgca
    31141 aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc gcttgggtcc tgttttttcg
    31201 ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg atcgtagttc ttcggcttgg
    31261 tcaccagcag cccttccgct gattcgcccg caatgttaga cagcgaaacg ttagccacac
    31321 cttccggccc cataaactga gttttcagcc ctgccgcgcg tgcctgacgc aggatttgcc
    31381 ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat attctctttt ttcagacgcg
    31441 ccaccagcgt tgagaaatct ttttccccgg cggtgatacc atcaaagaac accacgttag
    31501 cattgccttt cttcaggccg tcctgcaccg ctcgtgccag accttcgccg tattgctgtt
    31561 tgtcgtgaac gatagcaata cgctgcggtt tcactttctc aagaatatat ttcgccgccg
    31621 tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat cagctgatag ccacgggcgg
    31681 tcagctccgg cgcggttgcc gctggggtga tcattaaaat gccttcgtct tcgtagatgt
    31741 cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat cacatattta ataccgtcgt
    31801 taacgacttt gttcgccacc gcaaccgcct gtttcgggtc acaggcatcg tcatatttta
    31861 cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt gatatccgca accgcctgct
    31921 ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg accggacatt gcacccacga
    31981 ccgcgacttt aatatcttct gccagagcca tattgctgaa tgccagcgcg atacatcctg
    32041 ccagtaacgc tttacccttt atgttcatcc tgagaatccc cattcttctg gttattacgt
    32101 gtgttgtgat gttgttgttc agcactttat ttcgttttat gcatgactac ccgtgcttta
    32161 gcagcatact ctgctaaaac atacccgatt tttatgatat tggaatagct attttgacag
    32221 tttattaaca atctgcgtgg ggattggcgt tttgccggag ggggaattga ttattgtaga
    32281 ggcccaaaaa acaaaaaccc cggactctca tccagggttc tctgcttaat agcggaaatt
    32341 acgcttcaat agcggcgcga agttttttca tcgcgttctt ttccagttgg cgcacacgtt
    32401 cagcggaaac accgtaacgg tcagccagtt cctgcaacgt ggacttgttg tcttcgtcca
    32461 gccagcgcgc acggatgatg tcctggctgc gttcgtccag accatgcatc gcgtcggtca
    32521 gacggtttgc cgcctgctct tcccagttat catcttcgat gccgtcggca aagttagatg
    32581 atttatcctg cagatagagc accggtgcca tcggctggct gtcggaatcg tcgtcggaag
    32641 acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc catctcacgt acgtctttgc
    32701 tggttacgcc cagttcacgg gccaccattt cgacttcatc ctggttaaac cagcccagac
    32761 gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg cgctttggtg gtcgcaactt
    32821 tgacgatacg ccagttacgc agaacgtatt cgtggatctc tgctttgatc cagtggacgg
    32881 cgaaggagac caggcgcaca cccacttccg ggttaaaacg gcgcactgct ttcatcaggc
    32941 cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc atagcccgca taattacgag
    33001 caatatgaac aacaaaccgc aggtgagaca ggatcagcgt tttagctgct tccagatcgc
    33061 catggtaatg cagcttttca gccagcgccc gctcctcgtc agccgacaac atcggccacg
    33121 cgttagctgc ccggatgtag gaatccaggt tgccaactgg ggctaaagct aaactttgca
    33181 ttttgtcagt cattcaaatc ctctcaatcg atatcttctg gcgcttcagt ggtagcaaca
    33241 actgtgccaa agcttaagag caacgaggtt atcattcact gttttatcag accgtgattt
    33301 tatccacaag ttcaatgcaa gcttgtgaat aaattacgca caaaatgtga catagagatg
    33361 aaataccggg aagagacaac ggggtctctt tccctgctac ggaacccatt gcagggaaag
    33421 agtataacac gcttttatta ttcaggcgta aagtggcgta aatgttgtac cgtggcaagc
    33481 cacgctgcca cccagccaat catcgagcat accagcagca atagcaggca ttcatcgaac
    33541 gataaaccat tgatatcaaa cttcgttccg aaaacctgtg ccacttccgc caccgctgac
    33601 gacaaacgca gtaccagaat ttctgacaaa attaatgaca acaatgcgcc agaaaatccc
    33661 agcagcgcac caccgtacag gaacgggcga aggatgaaac catccgtcgc accaatcagt
    33721 ttctgcacgt tgatggagtc acggcgagca aagatgctca gacgtacgct gttgccgatg
    33781 acgaggaaca ctgccgccac catcaacacg ccgatcatcg ccgaaacgcg cccaaccagc
    33841 ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca tccgcacttc gtcaatgccg
    33901 ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt ccgtcccctg aaaatcgagt
    33961 ttcgggatca ccaccgccac tgccggaagc gggttttctt ccagcatatc cagcgcacca
    34021 ccaaaaccag accagttacg gaactcgccc agtgcgtctt cacgagaaag atagttcact
    34081 ttctccacgc cttgctcggc ctgcaactgt gccaccacgc ccgcagcggc gtcatcgtcc
    34141 agcgtttttt gcaagtaaac agtgatttgc ggtgacggat aatactgcgt cgccgcctga
    34201 ttaacgtttt tgtacaccat ataacagacg ctgggcagcg tcagggagat ggcgataacc
    34261 atcaccgtta aaaacgtggc gaacggtttg cttttcagat cctgcaatgc gccgtggaag
    34321 gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat ttaccggttt tggcgaggat
    34381 ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc cgccgaccga tttacggaag
    34441 cgatccagac gcccaccaaa ctgccgaata tgattgattg catcgcgctt attcatggcc
    34501 cacgcctcca tgcaagtgac catcgctcag ggtgagcatg cgataggaac gccgtgaaat
    34561 caggttaatg tcgtgcgttg ccatcaatac ggttacccca acgcggttaa attcttcaaa
    34621 cagacgtaga atcccttccg acaacgcgtc gtccaggtta ccggtcggtt cgtccgccag
    34681 cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca acacgctgtt gttcaccgcc
    34741 ggaaagctga atagggaagt tcttcgcttt gtccagtagc ccgactttat ccagtgccgc
    34801 cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg ataatcagcg ggatcgccac
    34861 gttatcgtag acagtacggt ccatcagtag atggtgatcc tggaaaatca tgccaatctg
    34921 gcggcgcaga aacggaactt cacggttttt cagacgcgtg atgtcatggc cgctaaacca
    34981 gattttcccg gcgctgggcc gctcaatccc acagatcagc ttcaggaggg tacttttccc
    35041 tgcgccggaa tgaccggtca gaaacgccat ctcacccggc tgcatatgga acgtaacgcc
    35101 ctgcagcgcc tgtctcccac cgagataagc cttgctgaca tgttcaaagc gaatcattgt
    35161 taatcctctc gggcaaaaag tgcctctata aagtcgtccg ccttaaacgg acgcaaatcc
    35221 tcaatacgtt cgccgacacc aatgtagcgg atagggatac caaactggtc agccaccgag
    35281 aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta gcgtgatgcc ggttaagcca
    35341 acagcttcgt ggaacagttt ggcctggctt accgcgttct gcccggtgct ggcatcaata
    35401 gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct tcatcacgcg gacgattttc
    35461 ttcaactctt ccatcagatg cgatttgttc tgcagacgtc cggcggtatc ggcaatcagg
    35521 acgtcgatat tacgcgcttt agctgcctga atggcgtcga agataacaga ggcggaatcc
    35581 gcaccggtat gctgggcaat caccggaata ttgttgcgct gaccccagac ctgaagctgt
    35641 tcaaccgcag ctgcacggaa agtatcgccc gccgccagca tcaccgattt accctgctgc
    35701 tcaaactgac gcgccagctt accaatcgtc gtggttttac ccacaccgtt gacgcccacc
    35761 atcaggatca cgaacggcgt tttatcttca acattcagcg gctcatcgac tttcgccaga
    35821 atctcaccca tctcttcttt cagcaggcca tagagcgcct cggcatcacg aagctgcttg
    35881 cgggatgcgc cttccgtcag attggtgata attttacgtg tggtttccac gcccacatcg
    35941 gcgatcaaca gctgttcttc cagctcttca aacagatcat cgtcgatttt tttaccgcgg
    36001 aacaggctga taaatccgga accgagattt tctttggttt ttaacaggct gcgtttcagg
    36061 cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct gggcaatttc ttcaaccggc
    36121 tgttcttctt ctgccggagg aactaccatc accgcctctt ctgccgcttc ggcagccagc
    36181 gcctgtgctt ccagctcttc gtcggtgatt tcttctttag ccgcttcttc ttctgccgct
    36241 tcgacaatct ctacggtttc cgcttcagcc tgccactctt ctggcgaaac ctcttcggcg
    36301 ttgacgtctt ccggcaacgg cagctcttca cgttcgatag ccactggctc cggcgtttct
    36361 tctacgaccg gttccggctg tgcaacaact tccgcttcag gctgcgcttt ttcactttca
    36421 acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag tttccgcctc ggcttcagta
    36481 tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct tcgcaggctc ttgcgcctga
    36541 acgatttctt ctacaaccgg ttgttcattc tgaacttctg tctctttttc cggggtctgc
    36601 tctttttgac caaagcccag ccaggaaaaa aagccacgtc ttttttcttt cgccatttgc
    36661 gactacactc ctcgctgttg cttcatggca cagcgttaac gctatgtaca tggcagctaa
    36721 aaaaatgatg aaatagtcta tcacttaact taattcacat caccgcctgc aagtatatgt
    36781 tatctggcgg attgagcaat ttatcatgaa aaaaccgaat cattccggca gcggccaaat
    36841 ccgcattatt ggcgggcagt ggcgaggccg taaactcccg gttcctgata gcccaggtct
    36901 gcgccccacc accgaccgcg tacgcgaaac gttgtttaac tggctggctc cggtcattgt
    36961 tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg ctggggctgg aagcgttatc
    37021 gcgctacgct gcgggggcaa cgttgattga gatggatcgc gtggtttctc agcagttaat
    37081 taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg gtgaacagca acgcaatgtc
    37141 attcctggcg caaaaaggca caccgcataa tatcgtgttt gtcgatccac cgttccgccg
    37201 tggcttgtta gaagagacga taaatttact ggaagataac ggctggctgg ctgacgaagc
    37261 cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc actgttccgg caaactggtc
    37321 attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg ctgtatcaac acgaagcaca
    37381 aggagaaagt gatgctgatt aatattggtc gtttgttaat gctctgcgtt tggggatttt
    37441 taatcctcaa tctggtgcat cccttcccgc gcccgctaaa tattttcgtt aacgtggcgc
    37501 tgatttttac cgtgctgatg catggtatgc agctggcgct attgaaatcc actttaccga
    37561 aagatggccc gcagatgacc accgccgaaa aggtgcggat tttccttttc ggcgtgtttg
    37621 aactgctggt ctggcagaag aaatttaaag ttaaaaaata accttattgt tcgccaacaa
    37681 agctaacaaa gcgcgttcct ttatagctca gcgtaccttt atcgcccacg gtcagggcgt
    37741 ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc tccgctttgc ggtttgaagc
    37801 ttgcctcata gcgcatactg gtgcctgccg gagtcacttc ctgctggcgc gaacggcgat
    37861 cgttaatcgg tttttcccgc ttgttgctca ccaccaccag cttttgctgg agcggagcca
    37921 tatcattatc agctttttcc cgtcgctgtt gcataaaacg aaacgatgcg gcgacgacaa
    37981 ttaagccaat gataacaata aagaaaagag gtggtttact catctttatc cctcatcgga
    38041 aaatgcggaa ttaagcatac cctgccagtt atggtgttgt catccgtcca ccctcgccac
    38101 taaactggaa gcaagaccgt aggcattccg cttacgaaaa aataacgaat tcaaggaact
    38161 aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat ggctatctgt ggatgcatcg
    38221 tatcgtgggc caacctggca acgctgggtg tttaaaccgt taacccttct tctcctgctg
    38281 ttactggcct ggcaagcacc gatgttcgac gccattagct atctggtgct ggcgggactg
    38341 tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac gtcaacgtct gatgtacgcc
    38401 atcggcgcgt ttttcctctc gcacctgctg tacaccatct atttcgccag tcagatgacg
    38461 ctctctttct tctggcctct gccactggtg ttgctggttc tgggtgcgct gctactggcg
    38521 attatctgga cgcggctgga agagtaccgt tggcctatct gcacgtttat cggcatgacg
    38581 ctggtgatgg tgtggctggc aggtgaactg tggttcttcc gtccgaccgc tccggcgctc
    38641 tctgcgtttg ttggcgcttc gttgctgttt atcagtaact ttgtctggct ggggagccac
    38701 tatcgccgac gcttccgtgc ggataacgcg attgctgcgg cctgctactt tgccggtcac
    38761 ttcctcatcg tccgctcact gtatctctga taaaacttga ctctggagtc gactccagag
    38821 tgtatccttc ggttaatgag aaaaaactta accggaggat gccatgtcga cacctgacaa
    38881 tcacggcaag aaagcccctc aatttgctgc gttcaaaccg ctaaccacgg tacagaacgc
    38941 caacgactgt tgctgcgacg gcgcatgctc cagctcgcca acgctctctg aaaacgtctc
    39001 cggcacccgc tatagctgga aagtcagcgg catggactgc gccgcctgtg cgcgcaaggt
    39061 agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg caggtgttgt tcgccaccga
    39121 aaaactggtg gttgatgccg acaatgacat ccgtgcccag gttgaatctg cggtgcaaaa
    39181 agcgggctat tccctgcgcg atgagcaggc cgccgacgag ccacaagcat cgcgcctgaa
    39241 agagaatctg ccgctgatta cgcttatcgt gatgatggca atcagctggg gtctggagca
    39301 gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc acgctagttg ggctgtaccc
    39361 gattgctcat caggcattac ggctgatcaa atccggcagc tacttcgcca ttgaaacctt
    39421 aatgagcgta gccgctattg gtgcactgtt tattggcgca acggctgaag ctgcgatggt
    39481 gttgctgctg tttttgattg gtgaacgact ggaaggctgg gccgccagcc gcgcgcgtca
    39541 gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc acgcgcctgc gtaacggtga
    39601 gcgggaagag gtggcgatta acagcctgcg gcctggcgat gtgattgaag tcgccgcagg
    39661 tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt gccagttttg atgaaagcgc
    39721 cctaaccgga gaatccattc cggtggagcg cgcgacgggc gataaagttc ctgcaggagc
    39781 caccagcgta gaccgtctgg taacgctgga agtgctgtca gaaccgggtg ccagcgccat
    39841 tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc cgcgccccca ttgagcggtt
    39901 tatcgaccgt ttcagccgta tttatacgcc agcgattatg gccgtcgccc tgctggtaac
    39961 gctagtgccg ccgctgctgt ttgccgccag ctggcaggag tggatttata aaggtctgac
    40021 gctgctgctg attggttgcc cgtgtgcgtt agttatctca acgcctgcgg cgataacctc
    40081 cgggctggca gcagcagcgc gtcgtggggc gttaattaaa ggcggtgcgg cgctggaaca
    40141 actaggtcgt gttacccaag tggcgtttga taaaaccggt acgctgaccg tcggtaaacc
    40201 gcgcgttacc gcgattcatc cggcaacggg tattagtgaa tctgaactgc tgacactggc
    40261 ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa gccatcgtac gcgaagcaca
    40321 ggttgctgaa ctcgccattc ccaccgccga atcacagcgg gcactggtcg ggtctggcat
    40381 tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct gccgggaaac atcccgctga
    40441 tgcatttgct ggtttgatta atgaactgga aagcgccggg caaacggttg tgctggtagt
    40501 acgtaatgat gacgtgctgg gtatcattgc attgcaggat accctgcgcg ccgacgctac
    40561 aactgccatc agtgaactga acgcgctggg ggttaaagga gtgatcctca ccggcgataa
    40621 tccacgcgca gcggcggcaa ttgccgggga gctggggctg gagtttaaag cgggcctatt
    40681 gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa catgcgcccc tggcgatggt
    40741 cggtgatggt attaacgacg cgccagcgat gaaagctgcc gccatcggga ttgctatggg
    40801 tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca ttaacccata accacctgcg
    40861 cggcctggtg caaatgattg aactggcacg cgccactcac gccaatatcc gccagaacat
    40921 caccattgcg ctgggactga aagggatctt cctcgtcacc acgcttttag ggatgaccgg
    40981 gttgtggctg gcagtgctgg cagatacggg tgcgacggtg ctggtgacgg cgaatgcgtt
    41041 aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca ttgagcgcga tcggtcccct
    41101 cgcccctctg gggagagggt tagggggtga ggaaaaggcg gcatcgaagc caatcagccc
    41161 ctatcaaccg cctttacgaa tcaaataacg ataaggcagt ccatccgtct ctttcgcaac
    41221 cagttcgtgt tccataaagg tacaaaaccc aggaatatcg cgggtagtgg ccggatcgtc
    41281 ggcgataatc agcaacgttt cgccaggctg catattgcgc acggttttgc gcaccatcat
    41341 caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg tggtcagggc tggaaaagag
    41401 atcggtcatt ttcttctcat cacttaaaaa aacggcgcta gtttacgccc tgtgagtccg
    41461 taagcaaacc aggttaacga ttgcgtgaaa attaaccatt gcattgtcaa cgtaaagcag
    41521 tatcatgcgg cggctcgaaa aaagggtaag cacgttatta tgttaaggta acagacgtgt
    41581 cgtacgtatt gggttccctc accccaatgg ttaatcaaaa aggtacaata tgaacgtttt
    41641 ctcgcaaact caacgctata aggcgttgtt ctggttatcg ttatttcatc ttctggtgat
    41701 cacctccagt aactatctgg ttcagcttcc cgtctccatt tttggtttcc ataccacctg
    41761 gggcgcgttt agctttccgt ttatttttct tgctaccgac ctgaccgtgc gtatttttgg
    41821 cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc cctgcgttat taatctccta
    41881 cgtcatctcg tcgctattct atatgggttc ctggcaggga ttcggcgcac tcgcccactt
    41941 caacctgttt gtcgcccgta tcgccaccgc cagtttcatg gcctacgcgc tggggcaaat
    42001 cctcgacgtg cacgttttta accgcctgcg tcagagtcgc cgctggtggc tggcaccgac
    42061 agcgtccaca ctgttcggta acgtcagcga cacgctggcc tttttcttca ttgccttctg
    42121 gcgtagcccg gatgccttta tggctgaaca ctggatggaa atcgcgctgg tcgattactg
    42181 tttcaaagtg ttaatcagta tcgttttctt cctgccaatg tatggcgtat tactcaatat
    42241 gctgttgaaa agactggcag ataaatccga aatcaacgct ttgcaggcga gttaaaggtt
    42301 cgtaatcaga gttgtgataa gatggatgaa tgagccgtta tggccgttta tcgaaaggaa
    42361 gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc ctgctggtta tggggcttgc
    42421 ggcctgtgat gataaagaca ctaacgctac ggcgcagggt tcggtcgcgg aaagtaacgc
    42481 taccgggaat cccgtcaacc tgcttgatgg caagttaagt ttctcgctgc cagcggatat
    42541 gaccgaccag agcggtaagc tgggaacgca ggccaataac atgcatgtct ggtccgacgc
    42601 caccgggcag aaagcagtca tcgtcatcat gggcgatgat ccgaaagaag atctggcggt
    42661 gttggcgaag cgtctggaag atcagcaacg tagccgcgat ccgcagctgc aagtggtaac
    42721 caataaagcc attgagctga aaggtcacaa aatgcagcag ttagacagta ttatctccgc
    42781 gaaaggccag acggcgtact cttccgttat tctgggtaac gtgggtaatc aactgctgac
    42841 catgcaaatt acgctgccag ctgacgatca gcaaaaagcg caaaccaccg cagaaaacat
    42901 cattaatacg ctggttattc agtaagtttt aagatgatga ggcggcctca gggacgtgtt
    42961 ccggaggccg tttttttaat cgccacgtca gtaataacgc gattgcgacc agtcccgccg
    43021 ccgccagata aatcaccggt acgcccgccc agctcatcac cagcccagcc agtggtccag
    43081 tcacgccaag cgataaatcc ataaatacgg tgtaagttgc cagcgccgcc ccctaatttt
    43141 gctgcggaac cgcttttacc gccactacac ccaatgccgg gaacaccagc gaaaacccgg
    43201 cccccgccag taagacgccg attttcgcca tccacggcat agtcgccacg ccaaccagta
    43261 gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac gtttaagcca ccgatacggt
    43321 taatgccgtt agggaataac aaacgcgtac cgacaaacgc acagctaaac agcgtcagcg
    43381 cgaaagccgc accgtcccaa cctttagcgt cataaaacag cgtgataaag gtggcgatga
    43441 cgccaaatcc ggcggaagcc agtgccagcg ccataccgta cagccagacg cgcccaagca
    43501 ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac cgtcggacgc gggatcgcca
    43561 acaaaatggc caccagcgcc acgcccataa tgattaacgc taacgcctgc aagccgcccc
    43621 agtgataaaa cacgacgcct aacggcgcac ccatcgccat cgccccgtaa gtgacaatgc
    43681 cgttccacga aatcacccgc ccgatatgca gcgagccaac cactccaacg ccccataggg
    43741 tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat gacgcgcccc aggcaaagta
    43801 ataacaggct gatgacaggc agactggcgg ttaatcctgc cgtcagatac cccagaccgc
    43861 tcaaaaagca gccgcataaa ccgaagacga caatcttttt gggtcccagc aaatcggcgt
    43921 aacgtccggc atgagggcgg ctcagcaagg tggcgaaata ttgcaggctg ataaccaatc
    43981 ctgcccagaa ggcgctaaag cccatcacat catggacata gcccggtaat acagcgagcg
    44041 gcaacccgat ggtgaggtag ctggcgaagt taaacatgac aatggagaca atgcgcaaat
    44101 tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc gggcatgagg atcaccacat
    44161 ttttacaaca gtgtttcatt tttaccatgt gctgacgtga aaatcagcag taagaatcag
    44221 aatattgctg gcgtgactcc cgctacactt aacacaaaaa gtcacaagga agccccaatg
    44281 gaaacccctc aacccgataa aacgggcatg cacattctgc tcaagttggc ctcgctggta
    44341 gtgatcctcg cgggcattca cgcagcggca gatatcattg tgcagctgtt actggcgctg
    44401 ttttttgcca tcgtcctcaa cccgctcgtt acctggttta ttcgtcgggg agtacaacgc
    44461 cccgttgcca ttacgattgt agtggtggtg atgctgatcg cactaaccgc gctggtcggc
    44521 gtactggcgg catcgtttaa cgaatttatc tctatgctgc cgaagtttaa taaggagctg
    44581 acgcgcaaac tttttaaatt gcaggagatg ttgccttttc ttaatttgca tatgtcgccg
    44641 gagcgaatgc tgcagcggat ggactcggaa aaagtggtta ccttcaccac agcgctaatg
    44701 accgggcttt ccggggcaat ggcgagcgtg cttttgctgg tgatgaccgt agtttttatg
    44761 ctgtttgaag tgcgccacgt cccttacaaa atgcgttttg cgctgaataa cccacagatt
    44821 cacatcgcag gattacaccg cgcacttaaa ggcgtttcgc attatctggc gttaaagacg
    44881 ctactcagtt tatggacagg tgtcatcgtc tggctggggc tggcgctaat gggcgtacag
    44941 tttgcgctga tgtgggcagt actggcgttt ttgctcaact acgtgcccaa tatcggcgcg
    45001 gtaatttccg ccgtaccgcc aatgattcag gtgctgctgt ttaatggtgt ttacgaatgt
    45061 attctggtcg gcgcattgtt tttagtggtc catatggtca tcggcaatat tttagaacca
    45121 cggatgatgg gccatcgcct ggggatgtcc accatggtgg tatttctttc attgttaatc
    45181 tggggatggc tgctcggccc ggtagggatg ctactttcgg tgccattaac cagcgtgtgt
    45241 aaaatctgga tggaaacgac gaaaggtggc agcaaactgg cgattttact ggggccggac
    45301 agaccgaaaa gtcggttacc gggatgaggc gacaagtgat acgatacgca ctttcatttt
    45361 ccattaaacg ttggccctga tatgtatcgg atagttctgg ggaaagtttc gaccttaagc
    45421 gcagctccac tgccaccggg tttacgcgag caagcaccgc aaggtccacg acgcgaacgc
    45481 tggctggcgg ggcgtgcatt gctttcgcac acgctttccc cgctaccgga gatcatctat
    45541 ggcgaacaag gcaaacctgc attcgcgccg gaaacgccgc tatggttcaa cttaagccat
    45601 agcggtgacg atatcgctct gctgttgagc gatgaaggcg aagtcggctg cgatatcgaa
    45661 gtgattcgcc cgcgcgccaa ctggcgctgg ctggcgaacg ccgtgttcag cctcggggaa
    45721 cacgctgaaa tggacgccgt gcatcctgat cagcaactgg aaatgttctg gcgcatctgg
    45781 acgcgcaaag aagccatcgt taaacagcgt ggcggcagcg cctggcaaat cgtcagcgta
    45841 gatagcacct atcactcctc gctgtcagtc agccattgtc agctagaaaa tttaagcctt
    45901 gcgatctgca cccccacccc ctttacgctc accgccgaca gtgtgcaatg gatcgattca
    45961 gttaactgat ccgcccaccc gactgcccat ctattgatcc agaacaggta atcagtatga
    46021 cgaatactta aaatcgtcat acttatttcc gccatctatt ttaatccatt ggggttacca
    46081 tgctctccac actccgccgc actctatttg cgctgctggc ttgtgcgtct tttatcgtcc
    46141 atgccgctgc accagatgaa atcaccaccg cctggccggt gaatgtcggg ccactaaacc
    46201 cgcaccttta cacgcctaac cagatgttcg cccagagcat ggtttatgaa ccattggtga
    46261 aatatcaggc agacggttcg gtgatcccgt ggctggcaaa aagctggact cattcagaag
    46321 atggtaaaac ctggaccttc accctgcgtg atgacgtgaa attctccaac ggtgaaccgt
    46381 tcgatgccga ggcggcggca gaaaacttcc gcgcagtgct cgataaccgt caacgtcacg
    46441 cctggctgga gctggcaaac cagattgttg atgttaaagc actcaataaa acagagctgc
    46501 aaattaccct gaaaagcgcc tactatcctt tcctgcaaga actggccctg ccccgtcctt
    46561 tccgttttat cgctccctcg cagtttaaaa accatgaaac catgaacgga attaaagcgc
    46621 cgattggcac cggaccgtgg attttgcagg aatcgaaact gaatcagtac gatgtcttcg
    46681 tccgtaacga aaactactgg ggcgaaaagc cagcgattaa aaagatcacc tttaacgtca
    46741 tcccggaccc gactacccgc gcggtggcgt ttgaaactgg cgatatcgac ctgctgtacg
    46801 gaaacgaagg gttattaccg ctcgatacct tcgcccgctt tagccagaat ccggcttacc
    46861 acacccaact gtcacagccg ctcgaaaccg tgatgctggc gctcaatacc gccaaagccc
    46921 ccaccaacga gctggcagta cgtgaagctc ttaattacgc ggtaaacaaa aaatcgctga
    46981 ttgataacgc gttatatggc acccagcagg tcgccgacac cctgtttgcc ccttctgtgc
    47041 cttacgctaa tctcggcctg aaaccgcgcc agtacgatcc gcagaaagcg aaagagttgc
    47101 tggaaaaagc cggttggacg ctgcctgcgg gcaaagacat ccgcgagaaa aatggtcagc
    47161 cgctgcgcat tgaactgtcg ttcatcggca ccgatgcgtt aagcaaatcg atggcggaaa
    47221 tcattcaggc tgatatgcgc cagattggcg cagatgtctc gctgattggc gaagaagaga
    47281 gcagtatcta tgctcgtcag cgcgacggtc gttttggcat gattttccac cgcacctggg
    47341 gcgcgccata cgatccacac gccttcctga gttcaatgcg cgtaccgtcg cacgctgact
    47401 tccaggcaca gcaaggatta gccgacaagc cgctgattga taaagagatc ggcgaagtgc
    47461 tggcgaccca tgacgaaacg caacgtcagg cgctgtatcg cgacattctg acccgtctgc
    47521 atgacgaggc ggtttatctg cctatcagct acatctcgat gatggtggta tcaaaaccgg
    47581 agctgggtaa tatcccctac gcgccgatcg ccaccgaaat tccgttcgaa cagattaaac
    47641 cggtgaaacc ttaatgttgc gttacgtatt acgccgcttt ctgctgctga tcccgatggt
    47701 gctcgccgcc tcggtgatca tttttctgat gctgcgcctc ggtaccggcg acccggcgct
    47761 cgattatctg cgtttatcta acctgccgcc gacgccggag atgctggcct ctacccgcac
    47821 catgctcggg ctggatcagc cgctgtacgt ccagtacggt acctggttgt ggaaggcgct
    47881 gcatcttgac tttggtatct cattcgccag ccaacgcccg gtactggacg atatgctgaa
    47941 cttcctgccc gccacgctgg aacttgcagg tgcggcgctg gtattaattc tgctcacttc
    48001 cgtaccgctc ggtatctggg cggcgcgcca ccgcgatcgt ttgccggatt tcgccgtgcg
    48061 ctttatcgcg tttcttggcg tgtcgatgcc caacttctgg ctggcatttt tgctagtgat
    48121 ggcgttttcg gtgtatctgc aatggctacc cgcgatgggc tacggcagct ggcagcacat
    48181 cattttacct gcggtttcca tcgcctttat gtcgctggcg attaacgcgc gactgctgcg
    48241 cgccagtatg ctggacgtcg ccggtcagcg tcacgtcacc tgggcgcggc tacgcggcct
    48301 gaatgacaaa cagaccgaac gccgccatat tctgcgcaat gcctcgctgc cgatgatcac
    48361 cgccgtgggg atgcacattg gcgaattgat tggcgggacg atgattatcg aaaacatctt
    48421 tgcctggcct ggcgtcgggc gctatgcggt gtcggcgatt tttaaccgtg actatccggt
    48481 gattcagtgc tttacgctga tgatggtggt ggtttttgtg gtctgtaatt tgattgtcga
    48541 tttgctcaac gccgcgctgg acccgcgcat tcgtcgtcat gaaggagcgc acgcgtgaac
    48601 tttttcctct cttcccgctg gtcggtacgc ctggcgctga tcattatcgc cctgctggcg
    48661 ctgattgcgc tcaccagcca gtggtggctg ccgtacgatc cacaggcaat tgatttgccg
    48721 tcgtgcctgc tttcgccgga tgcgcagcac tggctgggca ccgatcactt aggtcgcgat
    48781 attttctcgc ggctgatggc agcgacccgc gtgtcgctcg gttcggtaat ggcctgcctg
    48841 ctgctggtgc tgatattagg gctggttatt ggcggcagcg ccgggttgat tggcgggcgc
    48901 gttgatcagg ccaccatgcg cgtcgccgat atgtttatga ccttcccgac ctcgattctg
    48961 tcgttcttta tggttggcgt gctcggcacc gggctgacca acgtaattat cgccatcgcc
    49021 ctgtcgcact gggcgtggta tgcacgcatg gtgcgcagct tggtgatttc actacgccaa
    49081 cgcgagtttg tgctggcgtc acggctttcc ggtgcgggcc atgtgcgggt gtttgtcgat
    49141 catctggcag gcgcggtgat cccttcgctg ctggtgctgg caacgctgga tatcggccat
    49201 atgatgctgc acgtcgcggg gatgtctttc cttggcctcg gtgtgaccgc gccgaccgcc
    49261 gaatggggcg tgatgattaa cgacgcgcgc cagtatatct ggacccagcc gctgcaaatg
    49321 ttctggccgg ggctggcgct gtttatcagc gtgatggcct ttaacctggt gggtgacgca
    49381 ctgcgcgatc atctggatcc tcatctggtg acggagcacg cacactaatg ccgcaacaga
    49441 ttgaactacg taatatcgcg ctacaggccg cgcagccgct ggtacacggt gtatcgttaa
    49501 ccctgcaacg cgggcgcgtg ctggcgttag tcggcggtag cggcagcggg aaatcattaa
    49561 cctgcgccgc gacgctgggc attttgcccg ctggcgttcg ccagacggcg ggggaaattt
    49621 tagccgatgg caaaccggtt tcgccttacg ccctgcgcgg catcaaaatt gccaccatca
    49681 tgcagaaccc gcgcagcgcc tttaatccac tgcacaccat gcacacccac gcgcgggaaa
    49741 cctgcctggc gttagggaaa cccgccgatg acgctacgct taccgctgcc atagaagcgg
    49801 tggggctgga aaacgccgcg cgcgtgctga agctgtaccc gttcgagatg agcggcggca
    49861 tgttgcagcg catgatgatt gcgatggcgg tgctgtgtga atcaccgttt atcatcgccg
    49921 atgaaccgac caccgacctc gacgtggtag cacaggcgcg catcctcgat ctgctggaaa
    49981 gcattatgca aaaacaagcg ccgggaatgc tgctggtcac ccatgatatg ggcgttgtgg
    50041 cgcgtctggc ggatgacgtg gcggtgatgt cacaaggtaa aattgtcgaa cagggcgatg
    50101 tagaaacgct gtttaacgcc cccaaacata cggtgacgcg cagcctggtt tccgctcatc
    50161 tcgccctcta cggtatggag ctggcatcat gaccttactt aacgtctgcg gcctttccca
    50221 tcactatgcc cacggtggat ttagcggaaa acatcaacat caggcggtgc tgaataacgt
    50281 ttccctgacc ctgaaaagcg gcgaaaccgt cgccctgctg ggacgcagcg gctgcggaaa
    50341 aagcaccctc gcgcggctgc tggtaggctt agagtctccc tcacaaggga atattagctg
    50401 gcgcggcgaa tcgctggcga aacttaatcg cgcccaacgt aaagccttcc gccgtgatat
    50461 tcagatggtg tttcaggact ccatcagtgc cgtgaatccg cgcaaaaccg tgcgcgagat
    50521 cctgcgtgaa ccgatgcgcc acctgctatc actgaaaaaa tccgaacaac tggcgcgcgc
    50581 cagcgaaatg ctgaaggcgg tcgatctcga tgacagcgtt ctcgacaaac gcccaccgca
    50641 gttaagcggc ggccagctcc agctcgtctg cctggcacgc gcgctggcgg tcgaaccgaa
    50701 attgctgatt ctggatgaag ccgtttctaa ccttgatctc gtgttacagg cgggcgtcat
    50761 tcgcctgctg aaaaatctac aacaacagtt tggcactgcc tgcctgttca tcactcatga
    50821 cttacgcctg gtggaacgct tttgccagcg agtgatggtg atggacaacg gacaaatcgc
    50881 cgaaacccag gcggtgggag ataaattaac cttttcctct gacgccggac gtgtgctaca
    50941 aaacgcggta ttacccgcat tccccgtgcg ccgtcgcacc tcagaaaagg tttaacgcca
    51001 atgcaacgag tcaccatcac gcttgatgac gatttactgg agacgctgga cagcctgagc
    51061 cagcgtcgtg gttataacaa ccgttccgaa gctatccgcg atattctgcg tagtgccctg
    51121 gcgcaggaag ctacccagca gcacggcaca caaggtttcg cggtgctgtc gtatgtgtat
    51181 gaacacgaaa aacgcgactt agccagccgt attgtttcca cccagcatca tcaccacgac
    51241 ctctccgtcg ccacgctaca tgtgcacatc aaccacgacg actgtctgga aatcgccgtg
    51301 ttgaaaggtg acatgggtga cgtgcagcat tttgccgatg acgttatcgc ccagcgcggc
    51361 gtgcggcacg ggcatttgca gtgcctgccg aaggaagatt gagttatttc taaaacaagg
    51421 aaagaaaaga tattaactga acttaattta taaaattaag ttcagttgca tagatagatc
    51481 ctgtaaaggc agatattctg cgtaatgtac gtcgctaaaa tttataaatg attcaaacgc
    51541 tcattcagtg tttcaataat ataggtatta acagagactt gttgctgcgc agccgcatta
    51601 ttaaggcgtt cactcagcga ttctggatag cgtaacgtaa aggtcttaat cttttctgta
    51661 cgcgcatacg gctcaatacc cgcagcttta caatcctcaa gatactctcg cagggaaagc
    51721 tctccttctt tttgcagccc ctgaatactg tcagagacaa aatcacaata acctgacaag
    51781 cccaggaatt tgccacgaaa tgcattcagt tctggcacgt aggtaataac agctggttgc
    51841 ccggcaatct ccatagagtt tggcgtcttc agtttgatca tggttttacc cctatactta
    51901 gtaaccagtc acgcacgctt tctacagcgc ctttatcggt atctggcgac ggatgaggcc
    51961 gatggaaaca cgcaacgctc atatttagta tgaacttgca acgcgaacct cttccttcct
    52021 taatttctcc gcctaatgct ttaaccagtg actctatatc agaccacttg atcccttgag
    52081 gaacaggtgt tttaaatatt tgatccaatg tatttttttg tttcttccgt aaagcctgta
    52141 ccttggttct catggcatgt ccttatgact tcactttatg acatcacagt atgaagtcaa
    52201 gtttcccttc cttacgccat tgtcccaatc gtcttcctga atcgcagcag tgcgatggtg
    52261 aaaaacgcgc cgccaattgc catcagcgtc aggaactgcg gccagacgat ttcgaatccg
    52321 gcaccccggt agaggatggc ctgcgcgagg ctaacaaagt gtgtcgtcgg catggtcagc
    52381 ataatgtcct gcaccatctg cggcatactt tcgcgcggcg tggatccacc ggaaagcatt
    52441 tgcagcggca gcagcaccag aatcaccagc agccccagtt gcggcattga acgcgctatc
    52501 gtccccataa aaatgccgat tgacgtggtg gcaaacagac tgagcgccac gcccagcata
    52561 aacagcggga tcgagccttc aatcggtacg cccagtacac ctttcaccat cagcaccagc
    52621 gataatcccg ataccaccag caccaccagc cccatcgacc agatcttcgc catcatgatc
    52681 tcaaacggcg ttatcggcat caccagtaaa tgttccaccg tgccgtgttc acgctcgcgg
    52741 atcagcgccg atccggtcaa tacaatcgcc agcatggtaa tgttgttgat gatcgccatc
    52801 accccgccaa accacgcggg atcaaggttc gggttaaagc gcatccgggt ttccagcgat
    52861 accaacggtt cgctgttatc acggtagcgc gcgacaaagc tgttcacttc accgttgata
    52921 atattctgga tatacccatt gccggtaaat gcctggctca tgcgcgtggc atcgacgttc
    52981 acctgaatat ccggctggcg tccggcgagg acatcacgct gaaaattagg cggaatattt
    53041 atcgcgaagg tatagcgtcc ggcgtccagt ccggcatcca tctcatcggc ggtgatcatc
    53101 tccggtggca aaaaccacgg acgatagaag ctgttaacga tccggttcga taactgcgat
    53161 tgatccatat cggcaatggc gatcggcgcg aggttcaacg atcctggcgt aacggtcgct
    53221 gacgaataca ccgacaccgt aaacgagaag acaatcagcg tcagcatcgc tttatcaccg
    53281 agcagactgc gcaactcttt gatacccaga ttaaaaatat tgcgtaactg gcgcatcatc
    53341 cctcctgttt tttcagcagc aggatgctta agcccatcac cagcgggatg gctatcagca
    53401 acgggataaa aagttgccac aaatcagtca gatccagcgc tttcgagaac gttccgcggg
    53461 cgatagtcag aaaatgactg gtcgggtaaa cctcgccgat ccaacgtcca ggcccttcca
    53521 gcgaagctac cggatcgatc atcccggaaa actgtgtcgc cgggatcaat gtgataatcg
    53581 ccgttccgaa aatggcggca atctggcttt tcataaaggt ggagatcagc agccccattc
    53641 cagtggcaat gatgatatac agcagcgccg ccaaggtgag cgtcaggaaa ctgcctttat
    53701 gcggtacgcc aaacacaaac accgacaggc cgcagagcag gaaaaagttc agcatcccca
    53761 gcgcgatgta cggcagctgt ttgccaagca aaaattcgct acgggtggtg ggggtcacgt
    53821 aaaggttgat aatcgaccct agctcttttt cccgcaccac gctaagggcg cttagcattg
    53881 acgggatcat catcagcaga agcgggatca ccgccggaac aatcgctggc aggcttttta
    53941 cgtccgggtt atagcgataa cgcgtctcaa tgttcatcag cccgctttgg ctggcgggtg
    54001 tcgattgtcg gctcgccaca tcctgaagcc aactctggtg cattgcctgc acgtaacctt
    54061 ttaccgtttc ggcacgactg ggcatcgctc cgtcgatcca gacgccgagt tccacaggcg
    54121 taccacgcgc gatatcgcgc ccgaaattgg gcgggatctc aatggccacc gtgatatcgc
    54181 ccgcacgcat ccgacgatca agctcgtcat aactggtgag cggcggctgt tcgataaagt
    54241 aacgggaacc ggagaggttg agtgtccacg cctggctact gacggtctgg tcgcggtcga
    54301 gcaccgcaaa gcgcaggttt tccacatcca tactgatgcc gtaacccatt atcagcatca
    54361 ggatcaccgt tcccatcagc gccagcgtcg aacgtactgg atcgcgtcgc agttccagcg
    54421 cttcgcggcg gctgtagcta aacagacggc gcaggctaaa tccctgacgc ggcgcgtggg
    54481 tggtgtcgtg taccacgggc ggcgcttcgg cttcgttgct ctgccctgcc gcttcctgca
    54541 aataggcgat aaatgcctct tccagactgg cggctccgcg tttctcaacc agttcctgcg
    54601 gtgtaccgct ggcaagcact tttccggcgt gcatcagtga gatgcggtcg caacgttccg
    54661 cttcgttcat aaagtgggtg gagatgaaga tagtcacttt gtcctggcgc gagagatcga
    54721 ccatcaactg ccagaacata tccctcgcca ccggatcgac accagaagta ggctcatcga
    54781 ggattaacat ctccgggcga tgaatcaccg ccaccgccag cgaaagccgc tggcgaatgc
    54841 cgagcggcaa tgactccggc agaacatctt caacgtcgtt gagcttaaaa cgctcgctca
    54901 tttcagccac tcgcgcggga atttccgctt ccgggatgtg aaacaaacgg gcatgtaact
    54961 ccaggttttg ccgcacggtg agttcgttat agagcgaaaa cgcctgcgac atatagccca
    55021 cacgacggcg ggtatcgata tcttttggat caaccggttg cccaaatagc cacgcctcac
    55081 cttcgctggc gggcagcagt ccggtaagca ttttcatggt ggtggattta ccgcagccgt
    55141 tcgaaccaag gaatccaaaa atctccccgc gtggaatgcg gaaattgacg tgatcaacgg
    55201 caacgaagga accaaaacgc atggtcagat cgcgcgcttc gatggcaatc tctgcgtttt
    55261 caggttgata cggtgggatc actaccgcct gatgcgcctg gcgttgcgct tgcggtaaca
    55321 gattaataaa tgcttcttcc agcgtggcgc tttgcgtttg ctgccgcagc tcttcggcgc
    55381 tgccggttgc cagcacttct ccggcattca tcgctaccag ccagtcgaag cgttcggcct
    55441 cttccatata ggcggtggcg accagcacgc tcatattgct ctgccgctgg cgaatactgt
    55501 cgatcagatc ccagaactgg gcgcgggaga gcgggtcaac ccccgttgtt ggctcatcaa
    55561 ggatcaacag ttccgggtcg tggattaacg cgcagcacag cccaagtttt tgcttcatcc
    55621 cgccggagag tttacctgcc ggacgatcgc gaaacggtgc taacccggtg ctggtcagca
    55681 gctcattaat tcgcacttcc cgctccgctt tatcgtgacc gaacaggcga gcgaaaaaat
    55741 cgacattttc atacaccgac aaggtgtggt agaggttttt gcccagcccc tgcggcatcc
    55801 aggcgatgcg cgggcagacg tctcggcgat gcttcgggtc gtgcatatcg ccacccagca
    55861 ccaccacatt gccctgctca atgacgcggg caccggaaat cagtgacaat aaactcgact
    55921 tcccgacgcc atccggacca atcagcccga ccatgcaacg ggccggaata tcgagagtga
    55981 tattgttcag cgcaacggtt tttccataat gctggctcac gcccgccagt tgcgcgacag
    56041 gcgggacagg aaccagttcc agatgcgtca ttgcggcaac ctcaccacga ggtcgtcagg
    56101 ccacggaagt tcttcattca cccgcaccca cgctacgccc ggtaaaccgg ttttgacata
    56161 ttccagatgc tgctggagta attccggtgg gatacgcgct ttgacgcgga acatcagttt
    56221 cagccgttca tcgctggttt cgacggtttt tggcgtgaac tgggcgacac tggcgacaaa
    56281 actgatggtt gcaggaatac gcagatctgg cgcggcatcg aggatcagcc gggcttcacc
    56341 gcccagtttc agcgtgcccg cctgttcggt tggcaggaag aaggtcatat agacgtcgct
    56401 gagatcgacc atattcagca cccgaccgcc tgccgccagc acttcccctg gctcggcaac
    56461 ccgatactgc acgcgtccgt cacgcggggc tttcagttca ctgtcatcga tatctgcggc
    56521 aatgcgccgt tcagtggctt gcgctgcttc gacccgagtt tgcgcctgaa tgatattggt
    56581 gcgtgctgct tctatagccg ctttagaagc cgaaacctgg gctttcgctg attccagcgc
    56641 agctcgggcg ctctcagcgg cggcgcggtc gtcatccagc tgttgcgcag aaatagcccc
    56701 tcgttgggcg agcgagcggg aacgcgtatg acgtttggca acagagtcca gttcggcctg
    56761 gcgttgattg accagcgact gtgcggcacg agtttcactt tgtcgttgct ccagcaaagc
    56821 ctgcgcggcg gcaacggcgc tttgtgcctc tttgatttgc gcgatggctt ccagtcgctg
    56881 ttcctgcaac acgcgagtat ccatcttcgc cagcacttcg ccttcgcgaa caaactggcc
    56941 ttctttcacc agaatggtgt cgatacgccc ggcagttttg ctggcgatat ccacttccgt
    57001 cgcttcaatg cgcccattgc tgacagcaaa gccttccggc acacccgccg ggcgcaacag
    57061 ccaccacgcc acgacggcag ccaccgccag tgccccgaca acccaccacg ccagatggcg
    57121 cttactctta tccataatcg acccgccata atccctgtaa gcaaacgacg cgtgttacaa
    57181 ctgccgccga acgacgttca gcggattctt ccatccggga taagaaggaa acgacaaata
    57241 cgaccagcgc gcagagcatt gagcgccaac ggggaaggct tagcgaaaac gggtagtaat
    57301 gttgtcatca tcagcattcc tggccgtaaa tgaaacacgc atactcataa gtgtgatcta
    57361 taaaactggc tgacaaaacg gtatccatcc tgttggatcg cgtttcacgc ctgttcagtt
    57421 gcaggaacag agcattcctt tttacgtttt atcaacagtc tcaaccagat agtaacatta
    57481 atattcatgt tgtctatggt tcagagctgt aacattgtgc gccttctaat caaaggcata
    57541 agtccatttc tgtttttttc atgctggtaa gacttccagg gagtggttat ctctggccct
    57601 gcaccagggt taatttggca attatgcatg ctgaacattc ttccagcatg tttactgtca
    57661 cgggcgttaa gacctaaaac tgtccccgtt tcacctatgc gttctctccc accatttccg
    57721 ttatgtgcaa caacttgatt tatacctaac atttgtgaac atgctaatag tgcttcaagt
    57781 ttaggtctgt aatctgtcga aacagaaaaa taacgattaa aggatcgaca agagatagcc
    57841 cggctagttt taaacttttt gccattaatt atagcatgat gctttttatt aataatttcc
    57901 gctaattcga ccggatcgag agcattggtc atttcatcaa tagagacagt tatcggtcct
    57961 ataatgtaac gttttcgctg atcatcaaaa gttatcccat gatgatttgc cattattttg
    58021 tatttggggt catagaaaca tacaggataa tcttttatcc cgtcgtaggt atcctcatca
    58081 agtggtttca tgcgcgtgaa gttttgcata tagttaccat tgcaattcgt ttcatgattt
    58141 ccagctaata catgaacatt tttattgata ctcattatgc gttttaacaa atcgatgata
    58201 aattgaacgc cacagatatt actaaatcga tcgccggttt ggtcgccaag aaacgtacat
    58261 ggcgtgactg cgtctttatt aataaccaaa taaggctgaa gactttctaa aacattatct
    58321 cttctgttca gtatactgat tattgtatta acattagtgt caagttgctc attaaagttc
    58381 tcccttagca atacgttgag aacttcatct tctatattaa gcaactggct aaggaaattt
    58441 tctccttcag ccatgattcc caaatgtcct gttgcaaaaa gcgcgtacaa aactgcaccg
    58501 actgagctat cggtatcgcc aaaataggtc ggcctggtac ttgtgggatc aacaggtgaa
    58561 caattggaga aataggttct gtcagcgaac tggggtttta taattgtttc gaatttaata
    58621 tcaaatacat tgtattgcga tatttcccag ttccagatgg gtttgtacga ctcagtaact
    58681 gcaacatcat tagcattatc atttactgtc atattattaa gtaaataaac aatgctgcac
    58741 caattaatat cggtattatc aagagggcaa ccgtgatttt tactattaat tgtcacaata
    58801 aactgataac agttattcct acgtagaata tgagcgcgca tgcccgatag catactatat
    58861 ttttctgaat gattcgtttg tatggtaaat gaaattttgt catcttgaat agattgttgg
    58921 aaacgttcta tttgccaatc atcaattttt tccacaactt caccgagcat tgtcctggca
    58981 atacttgata tatcatcacc tataccattt gcaatatacc caaacagttc tttagtgaag
    59041 ttggtaacat gctcttgcac catatcattt gttgcgattg tcgttgtact gccattagtg
    59101 cctgccacag ttccaatttt catcttttac ctcatattat tcaccgcgag gaatacatag
    59161 ttaacatttt atcttatgac atacacctca tattcgatgg gaaaataatt atattttgca
    59221 caaatcattc agccttaaag cataatatga aatttcttat ctcatagcag agaaagtccc
    59281 taaagattgt agaggcgtca tcagtacata cctctacaat cgattaagaa aacctatgaa
    59341 atactggcaa aattaaaaag agataccaca gcactcatga agttttattg aggttgaaaa
    59401 caactaagcc ctgggacacc ttttttatca ccacgttcgt taggtgctat cttccaagaa
    59461 tttagcccca tggtataatt atcaattttt tgatgctggt ctacccctaa tccataatgg
    59521 ccaaatttct gtttagtttt tttgagcgtc tgtccacatt tgaagatatc ttcaggtttt
    59581 ggtctgaaag cagtagtgtt attaaaataa ttttcatata caggtgtggg tgctatagca
    59641 ttgcttctaa ttagatggag aagatgatca tccatgtgtt gtttatgttt tttattaaat
    59701 atatttgcta attcctcagg atcaattggg tctttcatct gattaaatgg aacctgcaaa
    59761 gcaccgagac aataacattg actttcttca tttcttatta ttccgtgatg actggttaaa
    59821 actttagttt ctgagttata attacaaaca tcgagcgttt taattaagtt atacgtatca
    59881 ccggcagaga gtttataatt ttctagtcgt gcagtatagt taccattaaa attgatttca
    59941 tgatttccag ccagaacgat gacattttta ttaatacggc tatctttatt accctccata
    60001 tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt actaaatcta
    60061 tcgcctgtat gatccccaag ggggatacaa ggggttatcg catcgtcctt taaaaaaaca
    60121 tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta gagcaattta
    60181 ttatttttat gtatgtatgc agatgaagcc atgtcttcgt gttttaataa ttcacataat
    60241 aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatga ataaagtaca
    60301 gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg tgggttaatc
    60361 acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata gctatcaaac
    60421 ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt aaaattaccg
    60481 ctaagaagag taaagaaatt ttcaggtatg atagaaagcc cattaacttt ataaattatg
    60541 tctgatttat ctccggagaa attaataaaa taataaccat ccttacactc aatcttaact
    60601 gtaatgggag acgataattt tatcacatgg ttattgtctg gtataattgt aaagataaac
    60661 gtgttagtag tattatcggc aaaactatta tattcttgaa tatctataga tggctcacta
    60721 ttgtaaaacc atattttctc catctgaaga ctatgttcat ttaccctcca gtgaccatgt
    60781 tgcaggaaat agatgatatg catgccaaaa tcgttatcta tttgcgaatc attatccagt
    60841 ttatcaattt caactatctt tgaacgatgt gttttatcaa ccttgctatg gtgttgatga
    60901 ttattaactt tatctgaatg cttatcttta tggtttgaag ggatgtactt aaatatattg
    60961 tcaattgctt tcataaataa cccgcgatat ttttgacctg ttagtaaaaa ataaatgata
    61021 catgtctgaa agacatattt acgacgatat aaaacaataa tgcttatatg ttcgctgatt
    61081 ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca tccctggaag
    61141 acaattgtaa aaaaaacaac agaataagaa ttaatcttag gataaatatt tatttatcat
    61201 ggccttttga ccgctaaaaa atggcactcg gcacagtaaa ccccgctata gttatgctat
    61261 acatttgtct ttaatgatat ctcgtcgtta tattaacagg ctgaaattat cattaatgca
    61321 catttcaata ttagcaggga tacctgagga gtttaaagtg aacatatata tcgggtagct
    61381 tttcaaatta atccctttga ttataggctt aatttgcatc gcgttaggtg gctttgtgct
    61441 ggaaagttca gggcaacgtc acgggtcatg tgctgatttc tctggcggcc atatgcctgg
    61501 cattattcac taccgcattt attatcattt cgcagctcac gcgcggcgtt aatacgtttt
    61561 acaatacatt gttccccatt attggctatg cggggtcaat tatcaccatg atatggggtt
    61621 gggcactgtt agcaggtaat gatgtgatgg cagacgagtt tgttgccggc catgttattt
    61681 ttggcgttgg tatgattgcc gcatgtgtat cgacggtggc agcgtcgtcc ggtcactttc
    61741 tgctcattcc caaaaatgca gcggggagca agagcgacgg aacaccggta caggcttatt
    61801 cttcattaat cggtaattgc ctcattgccg ttcccgtttt actcaccctg ctcggtttca
    61861 tttggtctat tacgctgtta catagtgccg acataactcc gcattatgtc gccttgggtt
    61921 aaccgcaatc tgtgcctgtc taattggcct agtggccaca atcgtccatc aaacacgtaa
    61981 tacgttctca tccaaagagc actggctgtg gtgttattgg gttatttttc tcggctcagt
    62041 cacggtgctg caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc
    62101 cggcattatt ctcatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct
    62161 actggcactg gtctggagac gtacctgttc gttagccaac agaataccga tgataccggt
    62221 atttacctgt ctgttttgct tgtttttgtc atcattcctc gcggaaatcg cacaaactga
    62281 gctggactac tttattccat ctcgggttct ggttggatta agcgcagtat gttttacatt
    62341 gttctctatt gtttcaatat tagaggccgg ttctgcgaaa aaaaattaaa atttacataa
    62401 tcccagcgtt attcatatct taacgctggt tttttacgaa cttagtgaaa taatgtagaa
    62461 tataaaatta taattttata ttggagcaca tgtgttaatg atcaaccccg ggatactaaa
    62521 aatatagaca ttattaaact tgcttagcct cgcattgcct ttacctgtta aagatgcatc
    62581 gagaaaattg agtctgaagt tctcatcctt tatctgattt tccagtcccg aaactttggc
    62641 cttagcatta cctgagatcc gtccgttact atctattttg taggtaaaaa aatacgaatg
    62701 ggaaaatttc ctcggtgtat cttcatcagt taagccaaca atcgtaaaac taccgctacc
    62761 atcttttttt aaaatgactg aaaggttagc agagtagtat aaagaatgat ctgttttagc
    62821 atttcttgtt aacgtcgacg tacattcaag agacgttgtg ctaccatcac gagccttcca
    62881 gagagcaaaa aaacacacct gtagcaaaaa tacattttct tattagtcat ataatagcac
    62941 ctctgaacaa attgcataag tttcgccatt agaggatttt atacatcttg ataaataccg
    63001 ctgcccttcc ccaagcaaat aatgcatact atttacatca tatataaaat ttgcacctgg
    63061 cacacagggt aaatgctttt tcgaaatttc agcggcctct atttgagatt tagcgaggcc
    63121 aatctcgtta agattcgctg aaagatataa atcacaggtg tcaatcttac ctgatttaac
    63181 caatggataa tccgttatac tgtaggtatc tttaaaagcc acggcaaaca tttcaataat
    63241 aacaattact accacaagcg aaatcaaaat gcgaaaaaga ggaatactcc ttttgcgctc
    63301 tactttttca acaacatctt cttgtaaatg agaatcatca taagattgtt tacttattgt
    63361 tatgttttca ctgatgtaaa agcctacttt gggtatcgta agaatcacat tttcttcaac
    63421 accaatattt ttaaaacttt tgcgtaacag ggatatatat tggttcaatg tgttgtttga
    63481 cagatcaaat gaaaatctct ccaaaacgct atcaatgatt gagttcctgt caaccgctcc
    63541 accattactt tccagtaaca ctgccaaaat acaccccgcc gtagctggca aatttatctc
    63601 atcctgggta tttacattcg agagcgagca tttgttcgga tcaaaaataa tcgcgtcatt
    63661 gataatgaaa aaacatctcc gctccatgca ggcaacaaca caaaataaga ttttgtcatt
    63721 ttagcctatt cctggtggaa ctgacacaag aaactaaaat acaaatataa ccctgaaaaa
    63781 gtcatataaa acttatatcc acagaataaa aaaacaaaac atgtcagttt tatcaatata
    63841 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
    63901 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
    63961 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
    64021 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
    64081 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
    64141 tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
    64201 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
    64261 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
    64321 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
    64381 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
    64441 atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca
    64501 gatatactct ctcttcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
    64561 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
    64621 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
    64681 attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt
    64741 ttttataagt taaaaaaggc gggatatcaa atcccgccat gaaattaact aatcatctgc
    64801 attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct
    64861 gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca
    64921 tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg
    64981 tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg
    65041 aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc
    65101 catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat
    65161 ataccccttt cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat
    65221 tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca aaagctgtcc
    65281 ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga ttttcattta
    65341 cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat gcttccacca
    65401 aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga aactataaac
    65461 atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt tggtattttg
    65521 cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct tccgcactca
    65581 agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt tcagtgtctt
    65641 tgctataact aatcacagcg ccgaagggga cgggtttacc ctctttctgt tttaaggtta
    65701 ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct ccagctgtcg
    65761 gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt ttttcatcca
    65821 gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca cgaaaatcag
    65881 tactcagtcc cggccagcca ttaacgttaa cccccgttgc tccgggtgcc tctataaggg
    65941 ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata agtatcccac
    66001 cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg ctactaaact
    66061 gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc ggttgtgact
    66121 gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta ttctgacctg
    66181 ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt gaagacgcat
    66241 aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt aatttctgat
    66301 ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg gagaatgtta
    66361 tgcctctcca taccgtacta taactcaggc tggtagaccg cgtgtttcct gtgccccggg
    66421 aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg ggctgggata
    66481 aggatatcgt agagccatcg tgactattct tatctctttt tctgttccgt tcatcctgtg
    66541 cacttactgc ttcagtcagt gtggcataac ctgatgcata acggttcgtg gttatagac
//
LOCUS       DAJTNM010000008        65613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTNM010000008 DAJTNM010000000
VERSION     DAJTNM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.008"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="OO172_000610"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="OO172_000610"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5510904.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OO172_000611"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OO172_000611"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5510905.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OO172_000612"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OO172_000612"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5510906.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OO172_000613"
     CDS             complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OO172_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5510907.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OO172_000614"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OO172_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5510908.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OO172_000615"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OO172_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5510909.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="OO172_000616"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="OO172_000616"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5510910.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7612..10146
                     /gene="mrcB"
                     /locus_tag="OO172_000617"
     CDS             7612..10146
                     /gene="mrcB"
                     /locus_tag="OO172_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5510911.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLAAIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10366..12609
                     /gene="fhuA"
                     /locus_tag="OO172_000618"
     CDS             10366..12609
                     /gene="fhuA"
                     /locus_tag="OO172_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5510912.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FGENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12660..13457
                     /gene="fhuC"
                     /locus_tag="OO172_000619"
     CDS             12660..13457
                     /gene="fhuC"
                     /locus_tag="OO172_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5510913.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13457..14347
                     /gene="fhuD"
                     /locus_tag="OO172_000620"
     CDS             13457..14347
                     /gene="fhuD"
                     /locus_tag="OO172_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5510914.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14344..16326
                     /gene="fhuB"
                     /locus_tag="OO172_000621"
     CDS             14344..16326
                     /gene="fhuB"
                     /locus_tag="OO172_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5510915.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16361..17641)
                     /gene="hemL"
                     /locus_tag="OO172_000622"
     CDS             complement(16361..17641)
                     /gene="hemL"
                     /locus_tag="OO172_000622"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5510916.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLMTEL
                     VPTIDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17866..19287
                     /gene="clcA"
                     /locus_tag="OO172_000623"
     CDS             17866..19287
                     /gene="clcA"
                     /locus_tag="OO172_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5510917.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGVLVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19311..19376
                     /locus_tag="OO172_000624"
     CDS             19311..19376
                     /locus_tag="OO172_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5510918.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19369..19713
                     /gene="erpA"
                     /locus_tag="OO172_000625"
     CDS             19369..19713
                     /gene="erpA"
                     /locus_tag="OO172_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5510919.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19760..20383)
                     /locus_tag="OO172_000626"
     CDS             complement(19760..20383)
                     /locus_tag="OO172_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5510920.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20421..21221)
                     /gene="btuF"
                     /locus_tag="OO172_000627"
     CDS             complement(20421..21221)
                     /gene="btuF"
                     /locus_tag="OO172_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5510921.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21214..21912)
                     /gene="mtnN"
                     /locus_tag="OO172_000628"
     CDS             complement(21214..21912)
                     /gene="mtnN"
                     /locus_tag="OO172_000628"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5510922.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21996..23513
                     /gene="dgt"
                     /locus_tag="OO172_000629"
     CDS             21996..23513
                     /gene="dgt"
                     /locus_tag="OO172_000629"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5510923.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23643..25067
                     /gene="degP"
                     /locus_tag="OO172_000630"
     CDS             23643..25067
                     /gene="degP"
                     /locus_tag="OO172_000630"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5510924.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25222..26378
                     /gene="cdaR"
                     /locus_tag="OO172_000631"
                     /pseudo
     CDS             25222..26378
                     /gene="cdaR"
                     /locus_tag="OO172_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26467..26853)
                     /locus_tag="OO172_000632"
     CDS             complement(26467..26853)
                     /locus_tag="OO172_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5510925.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27167..27991)
                     /gene="dapD"
                     /locus_tag="OO172_000633"
     CDS             complement(27167..27991)
                     /gene="dapD"
                     /locus_tag="OO172_000633"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5510926.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28022..30694)
                     /gene="glnD"
                     /locus_tag="OO172_000634"
     CDS             complement(28022..30694)
                     /gene="glnD"
                     /locus_tag="OO172_000634"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5510927.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30756..31550)
                     /gene="map"
                     /locus_tag="OO172_000635"
     CDS             complement(30756..31550)
                     /gene="map"
                     /locus_tag="OO172_000635"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5510928.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31918..32643
                     /gene="rpsB"
                     /locus_tag="OO172_000636"
     CDS             31918..32643
                     /gene="rpsB"
                     /locus_tag="OO172_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5510929.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32901..33752
                     /gene="tsf"
                     /locus_tag="OO172_000637"
     CDS             32901..33752
                     /gene="tsf"
                     /locus_tag="OO172_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5510930.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33899..34624
                     /gene="pyrH"
                     /locus_tag="OO172_000638"
     CDS             33899..34624
                     /gene="pyrH"
                     /locus_tag="OO172_000638"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5510931.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34916..35473
                     /gene="frr"
                     /locus_tag="OO172_000639"
     CDS             34916..35473
                     /gene="frr"
                     /locus_tag="OO172_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5510932.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35565..36761
                     /gene="ispC"
                     /locus_tag="OO172_000640"
     CDS             35565..36761
                     /gene="ispC"
                     /locus_tag="OO172_000640"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5510933.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36950..37708
                     /gene="ispU"
                     /locus_tag="OO172_000641"
                     /gene_synonym="uppS"
     CDS             36950..37708
                     /gene="ispU"
                     /locus_tag="OO172_000641"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5510934.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37721..38578
                     /gene="cdsA"
                     /locus_tag="OO172_000642"
     CDS             37721..38578
                     /gene="cdsA"
                     /locus_tag="OO172_000642"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5510935.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWCADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38590..39942
                     /gene="rseP"
                     /locus_tag="OO172_000643"
     CDS             38590..39942
                     /gene="rseP"
                     /locus_tag="OO172_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5510936.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39972..42404
                     /gene="bamA"
                     /locus_tag="OO172_000644"
     CDS             39972..42404
                     /gene="bamA"
                     /locus_tag="OO172_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5510937.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42526..43011
                     /gene="skp"
                     /locus_tag="OO172_000645"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42526..43011
                     /gene="skp"
                     /locus_tag="OO172_000645"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5510938.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43015..44040
                     /gene="lpxD"
                     /locus_tag="OO172_000646"
     CDS             43015..44040
                     /gene="lpxD"
                     /locus_tag="OO172_000646"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5510939.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44145..44600
                     /gene="fabZ"
                     /locus_tag="OO172_000647"
     CDS             44145..44600
                     /gene="fabZ"
                     /locus_tag="OO172_000647"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5510940.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44604..45392
                     /gene="lpxA"
                     /locus_tag="OO172_000648"
     CDS             44604..45392
                     /gene="lpxA"
                     /locus_tag="OO172_000648"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5510941.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45392..46540
                     /gene="lpxB"
                     /locus_tag="OO172_000649"
     CDS             45392..46540
                     /gene="lpxB"
                     /locus_tag="OO172_000649"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5510942.1"
                     /translation="MTDQRPLTIALVAGEISGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46537..47133
                     /gene="rnhB"
                     /locus_tag="OO172_000650"
     CDS             46537..47133
                     /gene="rnhB"
                     /locus_tag="OO172_000650"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5510943.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47170..50652
                     /gene="dnaE"
                     /locus_tag="OO172_000651"
     CDS             47170..50652
                     /gene="dnaE"
                     /locus_tag="OO172_000651"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5510944.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50665..51624
                     /gene="accA"
                     /locus_tag="OO172_000652"
     CDS             50665..51624
                     /gene="accA"
                     /locus_tag="OO172_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5510945.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51723..53864
                     /gene="ldcC"
                     /locus_tag="OO172_000653"
     CDS             51723..53864
                     /gene="ldcC"
                     /locus_tag="OO172_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5510946.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53921..54310
                     /locus_tag="OO172_000654"
     CDS             53921..54310
                     /locus_tag="OO172_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5510947.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54375..55673
                     /gene="tilS"
                     /locus_tag="OO172_000655"
     CDS             54375..55673
                     /gene="tilS"
                     /locus_tag="OO172_000655"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5510948.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55722..55982)
                     /gene="rof"
                     /locus_tag="OO172_000656"
     CDS             complement(55722..55982)
                     /gene="rof"
                     /locus_tag="OO172_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5510949.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55969..56169)
                     /locus_tag="OO172_000657"
     CDS             complement(55969..56169)
                     /locus_tag="OO172_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5510950.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56335..56868
                     /locus_tag="OO172_000658"
     CDS             56335..56868
                     /locus_tag="OO172_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5510951.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAW"
     gene            56877..57299
                     /gene="arfB"
                     /locus_tag="OO172_000659"
     CDS             56877..57299
                     /gene="arfB"
                     /locus_tag="OO172_000659"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5510952.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57313..58023
                     /gene="nlpE"
                     /locus_tag="OO172_000660"
                     /gene_synonym="cutF"
     CDS             57313..58023
                     /gene="nlpE"
                     /locus_tag="OO172_000660"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5510953.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58178..59002)
                     /locus_tag="OO172_000661"
     CDS             complement(58178..59002)
                     /locus_tag="OO172_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5510954.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRNIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59055..60773)
                     /gene="proS"
                     /locus_tag="OO172_000662"
     CDS             complement(59055..60773)
                     /gene="proS"
                     /locus_tag="OO172_000662"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5510955.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60884..61591)
                     /gene="tsaA"
                     /locus_tag="OO172_000663"
     CDS             complement(60884..61591)
                     /gene="tsaA"
                     /locus_tag="OO172_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5510956.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61588..61992)
                     /gene="rcsF"
                     /locus_tag="OO172_000664"
     CDS             complement(61588..61992)
                     /gene="rcsF"
                     /locus_tag="OO172_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5510957.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62110..62925)
                     /gene="metQ"
                     /locus_tag="OO172_000665"
     CDS             complement(62110..62925)
                     /gene="metQ"
                     /locus_tag="OO172_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5510958.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62965..63618)
                     /gene="metI"
                     /locus_tag="OO172_000666"
     CDS             complement(62965..63618)
                     /gene="metI"
                     /locus_tag="OO172_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5510959.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63611..64642)
                     /gene="metN"
                     /locus_tag="OO172_000667"
     CDS             complement(63611..64642)
                     /gene="metN"
                     /locus_tag="OO172_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5510960.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64830..65405
                     /gene="gmhB"
                     /locus_tag="OO172_000668"
     CDS             64830..65405
                     /gene="gmhB"
                     /locus_tag="OO172_000668"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5510961.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15511 a        16251 c        18081 g        15770 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga ccctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacatga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tattcgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaacctgg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcgggtt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cgctgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccga acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaacccta accctctccc
     7501 tcaagggaga ggggaccgat cgagcacaaa ttttgagaga tatcttcttc tgtcttgtaa
     7561 cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag catggccggg
     7621 aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta
     7681 agccgtcgtc gttacgaaga tgacgatgat tacgacgatt atgatgacta tgaggatgaa
     7741 gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc
     7801 ggctggctat ggctactgct aaaactggct atcgtttttg ccgtgctgat cgccatttac
     7861 ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg gcaactgccc
     7921 gcggcagttt atggccgaat ggtcaacctt gaaccagata tgaccatcag caagaacgaa
     7981 atggtgaagc tgctggaggc aacacagtat cgtcaggtgt cgaaaatgac ccgtcctggc
     8041 gaatttaccg tgcaggccaa cagcattgag atgatccgcc gtccgtttga tttcccggac
     8101 agtaaagaag ggcaggtgcg tgcgcgtctg acctttgatg gtgatcatct ggcggccatc
     8161 gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg tctgatcacc
     8221 atgatctcct cgccaaacgg tgagcagcgt ttgtttgtgc cgcgcagtgg tttccctgat
     8281 ttgctggtgg atactttgct ggcgacagaa gaccgtcatt tttacgagca tgatggaatc
     8341 agtctctact ccattgggcg tgcggtgctg gcaaacctga ccgcaggtcg cacggtgcag
     8401 ggtgcgagta cgctgacgca acagctggtg aaaaacctgt tcctctccag cgagcgttct
     8461 tactggcgta aagcgaacga agcttacatg gcgctgatca tggacgcgcg ttacagcaaa
     8521 gaccgtattc ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa
     8581 atccgcggct tcccgctggc aagcttgtat tactttggtc gcccggtaga agagctaagc
     8641 ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta caacccgtgg
     8701 cgtaacccga aactggcgct ggagcgacgt aatctggtgc tgcgtctgct gcaacagcaa
     8761 cagattattg atcaagaact ctatgacatg ttgagtgccc gtccgctggg ggttcagccg
     8821 cgtggtgggg tgatctctcc tcagccagcc tttatgcagt tggtgcgtca ggagttgcag
     8881 gcaaaactgg gcgataaggt aaaagatctc tccggcgtga agatcttcac tacctttgac
     8941 tcggtggccc aggacgcggc agaaaaagcc gccgtggaag gcattccggc actgaagaaa
     9001 cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt tagtggtgaa
     9061 gttcgtgcga tggtcggagg ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag
     9121 gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt atctgacggc tttaagccag
     9181 ccgaaaatct atcgcctgaa cacgtggatt gccgatgcgc caatcgcgct gcgtcagccg
     9241 aatggccagg tctggtcacc gcagaacgat gaccgtcgtt atagcgaaag cggcagagtg
     9301 atgctggtgg atgcgttgac acgttcgatg aacgtgccga cggtaaatct ggggatggcg
     9361 ctggggctgc ctgcggttac ggagacctgg atcaaactgg gcgtaccgaa agatcagttg
     9421 catccggttc cggcaatgct gctgggggcg ttgaacttaa cgccaatcga agtggcgcag
     9481 gcattccaga ccatcgccag cggtggtaac cgtgcaccgc tttctgcact gcgttcggta
     9541 atcgcggaag atggtaaggt gctgtatcag agcttcccgc aggcagaacg cgctgttccg
     9601 gcgcaggcgg cgtatctgac actatggacc atgcagcagg tggtacaacg tggtacgggt
     9661 cgtcagcttg gggcgaaata cccgaacctg catctggcag ggaaaacagg gactaccaac
     9721 aataacgtag atacctggtt tgcaggcatt gacggcagca cggtgaccat cacctgggta
     9781 ggccgtgata acaaccagcc gaccaaactg tatggagcca gcggggcaat gtcgatttat
     9841 cagcgttatc tggctaacca gacgccaacg ccgctgaatc ttgttccgcc agaagatatt
     9901 gcagatatgg gcgtggacta cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg
     9961 ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca gcagcagccg
    10021 tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac agccgcaaca gcaacctgct
    10081 cagcaagagc agaaagacag cgacggtgta gccggttgga tcaaggatat gtttggtagt
    10141 aattaacatc taagcgtgaa ataccggatg gcgagttgcc atccggtaaa ataacatccc
    10201 atctaagata ttaacccttt cttttcatct ggttgtttat taacccttca ggaacgctca
    10261 gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat tatcttatct ttataataat
    10321 cattctcgtt tacgttatca ttcactttac atcagagata taccaatggc gcgttccaaa
    10381 actgctcagc caaaacactc actgcgtaaa atcgcagttg tagtagccac agcggttagc
    10441 ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat caccgttacc
    10501 gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg caactattgc ggcgcggcag
    10561 tcagctaccg gcactaaaac cgatacgccg attcaaaaag tgccacagtc tatttctgtt
    10621 gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg taaaagaagc gcttagctac
    10681 acgccgggtg tctctgttgg tacgcgtggc gcatccaaca cctatgacca cctgatcatt
    10741 cgcggttttg cggcagaagg ccaaagccaa aataactatc tgaatggcct gaagttgcag
    10801 ggcaacttct ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc tgaaattatg
    10861 cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg gcggtctgtt gaatatggtc
    10921 agcaagcgtc cgaccaccga accgctgaaa gaagttcagt ttaaagccgg tactgacagc
    10981 ctgttccaga ctggttttga ctttagcgat gcgttggatg atgacggtgt ttactcttat
    11041 cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga agagcagcgt
    11101 tatgctattg caccggcgtt cacctggcgt ccggatgata aaaccaattt caccttcctt
    11161 tcttacttcc agaacgagcc ggaaaccggt tattacggct ggttgccgaa agagggaacc
    11221 gttgagccgc tgccgaacgg taagcgtctg ccgacagact ttaatgaagg ggcgaagaac
    11281 aacacctatt ctcgtaatga gaagatggtc ggctacagct tcgatcacga atttaacgac
    11341 acctttactg tgcgtcagaa cctgcgcttt ggtgaaaaca aaacctcgca aaacagcgtt
    11401 tatggttacg gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg tgcggcatta
    11461 gcgccagcgg ataaaggcca ttatctggca cgtaaatacg tcgttgatga tgagaagctg
    11521 caaaacttct ccgttgatac ccagttgcag agcaagtttg ccactggcga tatcgaccac
    11581 accctgctga ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc ctggtttggt
    11641 tacgacgact ctgtgccact gctcaatctg tacaatccgg tgaatcccga tttcgacttc
    11701 aatgccaaag atccggcaaa ctccggccct taccgcattc tgaataaaca gaaacaaacg
    11761 ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct aggcggtcgt
    11821 tatgactggg cagatcaaga atctcttaac cgcgttgctg ggacgaccga taaacgtgat
    11881 gacaaacagt ttacctggcg tggtggtgtt aactacctgt ttgataatgg tgtaacacct
    11941 tacttcagct atagcgaatc gtttgaacct tcttcgcaag ttgggaagga tggtaatatt
    12001 ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc ggaagatcgt
    12061 ccgattgtag ttactggtgc cgtgtataat ctcactaaaa ccaacaacct gatggcggac
    12121 cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg cgtagaaatc
    12181 gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac ttacaccgat
    12241 gcggaataca ccaccgatac tacctataaa ggcaatacgc ctgcacaggt gccaaaacac
    12301 atggcttcgc tgtgggctga ctataccttc tttgacggtc cgctttcagg tttgacgctg
    12361 ggcaccggtg gtcgttatac tggctccagc tatggtgatc cggctaactc ctttaaagtg
    12421 ggaagttata cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt cggcatggcg
    12481 ggctccaacg tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt cgccagctgc
    12541 tttaacactt atggctgctt ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttt
    12601 cgtttctaat ttctcttttg gggcacggat ttccgtgccc atttcacaag ttggctgtta
    12661 tgcaggaata cacgaatcat tccgatacca cttttgcact gcgtaatatc tcctttcgtg
    12721 tgcccgggcg cacgcttttg catccgctgt cgttaacctt tcctgccggg aaagtgaccg
    12781 gtctgattgg tcacaacggt tctggtaaat ccactctgct caaaatgctt ggtcgtcatc
    12841 agcctccgtc agaaggggag attcttcttg atgcccagcc gctggaaagc tggagcagca
    12901 aagcgtttgc ccgcaaagtg gcttatttgc cgcagcagct tcctccggca gaagggatga
    12961 ccgtgcgtga actggtggcg attggtcgtt acccgtggca tggcgcgctg gggcgctttg
    13021 gtgcggcaga tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta aaaccgctgg
    13081 cgcatcggct ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg atcgccatgc
    13141 tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc gacttcggcg ctggatatcg
    13201 cccaccaggt tgatgtgctg gcgctggtgc accgtttaag tcaggaacgt ggcctgacgg
    13261 tcattgccgt gttgcacgat atcaatatgg cggcacgcta ctgtgattat ctggtcgccc
    13321 tgcgcagtgg tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc ggcgaaaccc
    13381 tcgaaatgat ttatggcatc ccgatgggta ttttgccgca tccggcgggt gctgcacctg
    13441 tgagttttgt ttattgatga gcggcttacc tcttatttcg cgccgtcgac tgttaacggc
    13501 gatggcgctt tctccgttgt tatggcagat gaataccgcc cacgcggcgg ctattgaccc
    13561 caatcgtatt gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc tcggcatcgt
    13621 gccttacggc gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac caccattgcc
    13681 ggactcagtg attgacgtcg gtttgcgtac agaacctaac cttgaactgc tgaccgaaat
    13741 gaaaccgtcg tttatggtct ggtcggcagg atatggccct tcatcagaaa tgctggcacg
    13801 tattgcgccg ggtcgcggat ttaacttcag tgacggcaaa catccgttgg cgatggcgcg
    13861 taaatcgctg acggaaatgg cagatttact taacctgcaa agcgcagcgg aaacgcattt
    13921 agcgcaatac gaagacttta tccgcagcat gaaaccccgc tttgtgaagc gtggtgcacg
    13981 cccgttactg ctgacgacgc ttatcgatcc gcgccatatg ctggtcttcg gtccaaacag
    14041 cttgttccag gaaattctcg atgagtacgg catcccaaat gcctggcaag gggaaaccaa
    14101 cttctggggc agtaccgccg tcagtatcga tcgtctggca gcgtataaag acgttgatgt
    14161 gctctgtttt gatcacgaca acagcaaaga catggatgcg ctaatggcaa ccccgctgtg
    14221 gcaggccatg ccgtttgtcc gtacagggcg ctttcagcgc gtacccgcag tctggtttta
    14281 tggtgcaacg ctttcggcaa tgcactttgt gcgtgttctg gataacgcca tcggaggtaa
    14341 agcgtgagta aacgaattgc gcttttcccg gcgttattgc tggcgctgtt agtgattgtc
    14401 gctacggcgc tcacctggat gaacttctca caggcgctgc cgcgcagtca gtgggcgcag
    14461 gccgcctggt cgccggatat tgacgtcatc gagcagatga tttttcacta cagcttgttg
    14521 ccgcgtctgg cgatttcgct gctggtgggc gcgggcctgg ggctggtggg cgtgctgttt
    14581 cagcaagtac tgcgtaaccc gctggcggaa ccgacgacgt tgggcgttgc aacaggcgcg
    14641 caactgggga ttaccgtcac cacgctctgg gcgatcccta gcgcgatggc gagccagttt
    14701 gctgcgctgg caggggcttg cgttgttggc ttaattgtct ttggcgtcgc gtgggggaaa
    14761 cggctgtcgc cggtaacgct gattctcgcg gggttggtag tgagccttta ttgcggcgca
    14821 atcaatcagt tactggttat cttccatcat gaccagttgc aaagcatgtt tctgtggagc
    14881 actggaacgc tgacgcaaac cgactggggc ggtgttgagc gtttatggcc gcagttgctg
    14941 ggcggtgtga tgctgacgtt gctgctactt cgtccgttaa ccctgatggg gcttgatgat
    15001 ggcgtggcgc gcaatctcgg gctggccttg tcgcttgcgc gtctggcggc gctgtcgctg
    15061 gcgattgtca tcagtgcgct gctggtgaac gctgtgggga ttatcggctt tatcgggttg
    15121 ttcgcgccgc tgctggcaaa aatgctgggg gcgcggcgtc tgctgccacg actgatgctg
    15181 gcgtcgttga ttggtgcgct gatcctctgg ctttccgatc aaatcatcct ctggctgact
    15241 cgcgtgtgga tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc gccgctgcta
    15301 ctgtggctgc tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt taacgatcgt
    15361 gtcgcgactg aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt gctgttgttg
    15421 atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc acggctggac gtgggcgagc
    15481 ggggcgttgc tcgaggattt aatgccctgg cgctggccgc gaattatggc ggcgctgttt
    15541 gcgggcgtca tgctggcggt agcgggctgt attattcagc gactgaccgg caacccgatg
    15601 gcaagcccgg aagtgctggg gattagctcc ggcgcggcgt ttggtgtggt gttaatgctg
    15661 tttctggtac cgggtaatgc ctttggctgg ttgttacccg caggcagtct cggggcggcg
    15721 gtgatgctgt tgatcattat gatcgccgcc ggacgcggtg gattttcccc acaccgcatg
    15781 ttactggcgg ggatggcgtt aagtaccgcg ttcaccatgc ttttgatgat gttgcaggca
    15841 agtggtgacc cgcgaatggc gcaagtgctg acctggattt ccggttcgac ctacaacgcg
    15901 accgatgctc aggtctggcg caccggaatt gtgatggtga ttttgctggc gattactccg
    15961 ctgtgccgcc gctggctgac cattttaccg ctgggtggtg ataccgcgcg ggcggtaggg
    16021 atggcgctga cgccgacgcg aattgcgctg ctgctgttag cggcttgcct gacggcgacc
    16081 gcgacgatga ctattggacc gttgagtttt gttggtttaa tggcaccgca tattgcgcgg
    16141 atgatgggct ttcgacggac gatgccacat atcgtaattt cggcgctggt gggtggttta
    16201 ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt ttccattcca gatcccggcg
    16261 gggctgctgt cgacctttat cggtgcgcca tattttatct atttgctgag aaagcagagc
    16321 cgttaataga atgcctcgcc cggtgggcga ggcaaaatta tcacaacttc gcgaacaccc
    16381 gacgtgcagc atcgatggtg ttattgatat cttccatgct gtgcgccaca gacataaagc
    16441 ccgcttcaaa cgctgacggt gccaggtaaa caccttcatc cagcatcata tggaagaaac
    16501 gcttaaaacg ctcgacgtca caggccatca catcctgata gcacgtcacg gactcggcgt
    16561 cggtaaagaa gataccgaac ataccaccga cgtggttaac gaccagcgga attcccgctt
    16621 cttctgccgc ttccagcaga ccttctgcca gacgtgttgt cagctcatcc agcgtttcgt
    16681 gaatgcccgg ctgcgcgact tcattcagac aggcgaaacc cgctgccatc gcaatcgggt
    16741 taccggaaag cgtacccgcc tgatagaccg gacctgtagg ggccagcgca tccattacat
    16801 cacgacgacc accgaatgcg cctaccggca ttccaccgcc gatgattttt ccgaggcagg
    16861 ttaaatctgg cactacgccg aaataatcct gtgcgccagc tagcgctacg cggaaaccgg
    16921 tcatcacttc atcgatgatc agcaacgcgc caaattcgtc gcacagcgca cgcagacctg
    16981 gcaggaactc tggcagcggc ggaacacagt tcatattgcc tgccaccggc tcgacgataa
    17041 tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg tacagaggcc agatcgttat
    17101 aagtacaggt taaggtatgt ttggcgaaat ctgccggaac gcccggcgag tttggctggc
    17161 ctaacgtgag tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac
    17221 aaccttcaaa tttaatgatt ttgtcgcgac cggtaaaacc acgggccaag cggatggcgc
    17281 tcatcgtcgc ctcggtgccg gagttcacca tgcgcaccat atctatggtc gggaccagtt
    17341 cggtcatcag ttgcgccatt ttcacttcca tttcggtcgg tgcaccaaag cttaaaccac
    17401 gctcggcggc ttcaatcacg gcattgcgga tcgccggatg gttatggccc agcaccatcg
    17461 gcccccagga accgacataa tcgatatagg ctttgccatc aacatcgtac agataagcgc
    17521 cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg
    17581 agttcacacc gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca
    17641 tggagggttc ctgattcgta gaaaaagtga atggctgcta ttctatgtta ttcataacaa
    17701 gttaaatact cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta
    17761 taaaagtttt gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag
    17821 taaaatgccg tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact
    17881 ccctctttag aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt
    17941 cttgagcgcg ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt
    18001 atcgggctgg ctgcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg
    18061 ggagtgctgg tacatactgc tgataattat ccgctactgt taaccgtcgc ttttctctgt
    18121 tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc cgaagcaggg
    18181 ggatcgggga tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt tcgctggtgg
    18241 cgtgtattgc cggtgaaatt ctttggcggg ctggggacac tcggcggagg catggtgcta
    18301 gggcgcgaag gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt gcttgatatt
    18361 ttccgcctga aaggtgatga agctcgccat acgctgctgg caaccggtgc tgctgcgggg
    18421 ctggctgcgg cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt
    18481 ccgcagtttc gctatacgtt aatttcgatt aaagcggtat ttattggcgt cattatgtcg
    18541 accattatgt accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct
    18601 gatgcgccgc ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc
    18661 ggccctattt ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc
    18721 ggcaatatta ccaaatgggt actgatgggg ggggcgattg gcggtctgtg tggattgctg
    18781 gggtttgtgg caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg
    18841 gggaatttca gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat taccacctta
    18901 ctctgcttct cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact
    18961 gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcacctggag
    19021 gcggggacgt ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg
    19081 ttaacgggga tcattctggt tctggagatg accgataact accagctcat tttgccaatg
    19141 attattaccg gtcttggcgc aacactatta gcgcaattta ccggcgggaa acctctatac
    19201 tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg agcaactggc gcgaagcaag
    19261 gccgcatcag ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta
    19321 ttattgggtt agaatttgcc cgattgccga tgtcgtttgg agcaaaatat gagtgatgac
    19381 gtagcactgc cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct
    19441 gacgaagata acccgaatct gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc
    19501 ttccagtatg gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa
    19561 cagggcgttg gcctggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt
    19621 gactacaccg aaggactgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc
    19681 acctgcggtt gcggttcttc ctttagcatc taatctgttg ttcatgtgcc ggataaacta
    19741 tccggcacat cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa
    19801 gatgccaacg aatagccgcc agccgaatca atagcgtcac gaccatgccc atcatactgg
    19861 ctgtttccag tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa
    19921 tacaggcagt tgcgtagatt tctgtacgta aaatcatggg gatttcgcgg gccagtacat
    19981 cacgaatgat cccgccgcca acgccagtaa tgacgcccat acaaaccgcg attaacggac
    20041 cggcttccgc attaaaggct ttattcacgc caatgccgac aaacaccgcc agaccaacgg
    20101 cgtccagcac cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg
    20161 tcagcatgct ggtgaccatg gcaacgacca gatcggtggg atctttcacc caaaataccg
    20221 ggccgtgatc cagcgccatg tcgcgaattg ttccgtcgcc tactgcggta accacgccca
    20281 gtaccagaac accaaaaggg tccatacgca attttccggc taacaaaacg ccggagatgg
    20341 caaataccgc tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga
    20401 gcacctgaaa aggtcaggca ctaatctacc tgtgaaagcg cattacagag ctgttgtgct
    20461 gcgaggataa tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga
    20521 attttgagct gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta
    20581 atgacaatcg cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga
    20641 acccggctgt ctttaaagat gttttctccg ccacaaactt cgagtacctg gttctgaatc
    20701 gactcttttc cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta
    20761 ggtttatcag catattgcgc tttcaattgc acgtactgat ccagcaggga ttgcgcggct
    20821 tgttcggcct tgtctggttg cggactccag ggggccagtt gacgtaacgc attggcaatt
    20881 tgttcaatgc ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca
    20941 acctgccgct cggcattacc tccacgccag gcaatcacca gatcgggttt cagcgcgaca
    21001 atgcgttcca gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga
    21061 ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca
    21121 gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcgaga
    21181 aaagacaggg cgaccagcgc cctgaacagt gatttagcca tgtgccagtt tctgcaccag
    21241 tgactcaacc atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag
    21301 atgagactgt tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa
    21361 attgtggcag acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa
    21421 gttgtggcgg attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat
    21481 caggccacgt acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag
    21541 tttatcgtca gctttaaagc ctgtcggaca gcctggtaac tgaccgtatt cataaccaaa
    21601 tgccgtgaca tccgcgtcgt gataacgcgc ttcgtccgag acaacgatat cgcccacttt
    21661 cagcgttggt gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg
    21721 ttccaacagc agagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag
    21781 cgcaacctcg gttccattca gttggccggt atagatttcg caaccgccga gactgatagt
    21841 ttgacggttt tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat
    21901 gccgattttc atagatttac tcgcgataag cccgatttga aggcatagtt taccatgcgc
    21961 ttacggggaa gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa
    22021 aaataaactg gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc
    22081 tgcggatctt cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc
    22141 aacaaaagac ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc
    22201 actcgatgga agtccagcag gtgggacgct acatcgccaa agaaatttta agccgtctga
    22261 aagagcttaa attgctggaa gcatacggcc tggatgaact gaccggcccc tttgaaagca
    22321 ttgttgagat gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg
    22381 aagcagcgat aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc
    22441 ctctgactga cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaaccgc
    22501 ttaacgagct gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag
    22561 gcattcgtct ggtgcataca ttgatgcgga tgaatctcac ttgggcacag gttggcggta
    22621 ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt
    22681 taatgaaaaa gccgggttat tatctttctg aagaagccta tattgcccgg ttgcgtaaag
    22741 aacttaattt ggcgctttac agtcgttttc cattaacgtg gattatggaa gctgccgacg
    22801 acatctccta ttgtgtggca gaccttgaag atgcggtaga gaaaagaata tttaccgttg
    22861 agcagcttta tcatcatttg cacgaagcgt ggggccagca tgagaaaggt tcgctctttt
    22921 cgctggtggt tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc cgcagtacgg
    22981 aagatcagtt ttttatgtat ttacgggtaa acaccctaaa caaactggta ccctacgcgg
    23041 cacaacgatt tattgataat ctgcctgcga ttttcgccgg aacgtttaat catgcattat
    23101 tggaagatgc cagcgaatgc agcgatcttc ttaagctata taaaaatgtc gctgtaaaac
    23161 atgtatttag ccatccggat gtcgagcagc ttgaattgca gggctatcgg gtcattagcg
    23221 gattattaga gatttatcgt cctttattaa gcctgtcgtt atcagacttt actgaactgg
    23281 tagaaaaaga acgggtgaaa cgtttcccta ttgagtcgcg cttattccac aaactctcga
    23341 cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt accgtcagat tctcctgagt
    23401 ttccgctatg ggaatattat taccgttgcc gcctgctgca ggattatatc agcggtatga
    23461 ccgacctcta tgcgtgggat gaataccgac gtctgatggc cgtagaacaa taaccaggct
    23521 tttgtaaaga cgaacaataa atttttacct tttgcagaaa ctttagttcg gaacttcggg
    23581 ctataaaacg aatctgaaga acacagcaat tttgcgttat ctgttaatcg agactgaaat
    23641 acatgaaaaa aaccacatta gcactgagtg cactggctct gagtttaggt ttggcgttat
    23701 ctccgctctc tgcaacggcg gctgagactt cttcagcaac gacagcccag cagatgccaa
    23761 gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt cagcattaac gtagaaggta
    23821 gcacaaccgt taatacgccg cgtatgccgc gtaatttcca gcagttcttc ggtgatgatt
    23881 ctccgttctg ccaggaaggt tctccgttcc agagctctcc gttctgccag gggggccagg
    23941 gcggtaatgg cggcggccag caacagaaat tcatggcgct gggttccggc gtcatcattg
    24001 atgccgataa aggctatgtc gtcaccaaca accacgttgt tgataacgcg acggtgatta
    24061 aagtccagct gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa gatccgcgct
    24121 ctgatatcgc gctgatccaa atccagaacc cgaaaaaact gaccgcaatt aagatggcgg
    24181 attctgatgc actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg tttggtctgg
    24241 gcgagacggt aacttccggg attgtctctg cgctggggcg tagcggcctg aatgctgaaa
    24301 actacgaaaa cttcatccag accgatgcag cgatcaaccg tggtaactcc ggtggtgcgc
    24361 tggttaacct gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca ccggacggcg
    24421 gcaacatcgg tatcggtttt gctatcccga gtaacatggt gaaaaacctg acctcgcaga
    24481 tggtggaata cgggcaggtg aaacgcggtg agctgggtat tatggggact gagctgaatt
    24541 ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta agccaggttc
    24601 tgcctaattc ctcggctgca aaagcgggca ttaaagcggg tgatgtgatc acctcactga
    24661 acggtaagcc gatcagcagc tttgccgcac tgcgtgctca ggtgggtact atgccggtgg
    24721 gcagcaaact gaccctgggc ttactgcgcg acggtaagca ggttaacgtg aacctggaac
    24781 tgcagcagag cagccagaat caggttgatt ccagctccat cttcaacggc attgaaggcg
    24841 ctgagatgag caacaaaggt aaagatcagg gcgtggtagt gaacaacgtg aagacgggca
    24901 ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat tattggtgcg aaccagcagg
    24961 cagtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct gtgctggcac
    25021 tgaacattca gcgcggcgac agcaccatct acctgttaat gcagtaatct ccctcaaccc
    25081 cttcctgaat acgggaaggg gttctcctta caatctgtga acttcgccac aactccatac
    25141 atcttcatca tcctttgggc atttgcacaa tgccgtacgt tacgtacttc cttatgctaa
    25201 gccgtgcata acggaggact tatggctggc tggcatcttg ataccaaaat ggcgcaggat
    25261 atcgtggcac gtaccatgcg catcatcgat accaatatca acgtaatgga tgcccgcggg
    25321 cgaattatcg gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg tgcattgctg
    25381 gtactttcac agggacgagt cgtcgatatc gatgacgcgg tagcacgtca tctgcacggt
    25441 gtgcggcagg ggattaatct accgttacgg ctggaaggtg aaattgtcgg cgtaattggc
    25501 ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat gacggctgaa
    25561 atgatgctgg aacagtcgcg gttgatgcac ttgttggcgc aggatagccg tttgcgggaa
    25621 gaactggtga tgaacctgat tcaggcagag gagaatactc ccgcacttct gaatgggcgc
    25681 aacggctggg gatcgatctc aatcaaccgc gagtggtggc tattgttgag gtcgacagcg
    25741 gtcagcttgg cgtggacagc gcaatggcgg agttacaaca actgcaaaac gcgctgacta
    25801 cgcccgagcg taataatctg gtggcgattg tctcgctaac cgaaatggtg gtgttgaaac
    25861 cggcgttgaa ctcttttggg cgctgggatg cagaagatca tcgtaagcga gttgaacaac
    25921 tgattacccg catgaaagag tacggccagc tgcgttttcg cgtttcactg ggcaactatt
    25981 ttaccggtcc tagcagtatt gcccgatcct atcgtacggc gaaaacgacg atggtggtgg
    26041 gtaaacagcg gatgccagaa agtcgctgct atttttatca ggatctgatg ttacctgtgt
    26101 tactcgacag tttgcgtggc gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc
    26161 tgaaagcgat ggacaataac ggcttgctgc gacgaacgct ggcggcgtgg tttcgccaca
    26221 atgtgcaacc gctggcaacg tcaaaggcgt tgtttattca tcgtaatacc ctggagtatc
    26281 ggcttaatcg tatatcggaa ctgaccgggc ttgatttggg caattttgat gacaggttgc
    26341 tgctgtatgt ggcgttacaa ctggatgaag agcggtagat tatgcgttaa ggttgaggcg
    26401 gcgctggctc attcccctca gcccgaaggg gagaggggaa tgcccgagtt aaccgtcaat
    26461 tcgggattat ttacgcgtta gtttttccag atcggcttca atctcgctga tcttattggt
    26521 tacgactgac tccaggtgac gtaagtcgtc gaggatctta cgcttaagat caacttcgct
    26581 gcggtcacgc tgacagattt gatcaagctc atcaatgata taccgtagat tcgggctgat
    26641 ttcctggact tctttataac cctgacccac accatcagcg acgaccgttt tacgctgacg
    26701 cggatattta aacttaacgc tcttggcgaa aaactcgcct ttgtctttct ggaaatagat
    26761 tttcagaata tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt cttcaggatt
    26821 ggtaataccc agacttttca gattgtcgta catagcgtta cctcaaaatg agtcagtaaa
    26881 ttgtgcttat tttagcattt ggcttggccc gcccggctcg actgtgatcg cagaaagcct
    26941 ttttcttcca gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt tattgtggcg
    27001 atattccatg ggatggatta gcacatttat cttcgttttg cgaattacat gacagtccga
    27061 aacaagatga aaaagtattt aaaaaagggg gaggaagaat gagtaggacg aattattctg
    27121 agccagaaag catacgggca gcatcatgct gcccgtatat gtactcttaa tcgatggtac
    27181 gcagcagttc gttaatgccg actttgccgc gagttttcgc gtcaactttc ttaacgataa
    27241 ccgcacagta gaggctgtat ttgccatctt ttgacggcag attacctgaa acaaccacag
    27301 accccgccgg aacgcgaccg tagtggattt cgccggtttc acggtcgtaa atacgggtgc
    27361 tctgaccaat gtatacgccc atagagatga cggaaccttc ttcgacaatc accccttcaa
    27421 ccacttcaga gcgtgcgcca atgaagcagt tatcttcaat aatggtcggg ttagcctgca
    27481 gcggctccag cacgccgccg atgcccacgc caccggaaag gtggacgttt ttaccaatct
    27541 gcgcacaaga accgacggtc gcccaggtat caaccatggt gccttcatca acatatgcgc
    27601 cgatgttaac gtaagacggc atcagcacgg tgttacgggc aataaacgca ccctgacgta
    27661 ccgccgctgg tggcacaacg cggaagcctt ctttctggaa acgtgcttcg tcgtagtcgg
    27721 cgaatttcat cggcactttg tcgaagtagc ggctttctgc cccttcgatc acctgattat
    27781 cattaatacg gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc acccactgac
    27841 cgtcaatttt ttccgctaca cgcagtgcgc cggaatccag cagggcgatc acctgattta
    27901 ccgcttcgcg ggtaacggtg tctgcattgg ctggcgtgat ctcggcacgg cgttcaaaag
    27961 cggtttcaat aatgttctgt aactgctgca ttgttaaact cttttcatat cagtaaacac
    28021 atcacccttt atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact tcctgctgca
    28081 actcgttatt aagcgcacgc cggtcagcgg tggcaattat gaataaatct tctactcgct
    28141 cgccaatggt tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca aaaattttcc
    28201 cgactcgcgc cagcagtcca ggttggtcga gggcgatcag ttcgaggaac gatttgcggt
    28261 cggtatgggt cggcaaaaac gttacttcgg tttcaacagt aaaatggcgt aatttggcgg
    28321 gttggcgacg gggctgcggt ggctgccagc tactttgcgt cagcacctgc tccagaccaa
    28381 accgaataac ctcatggcga tctgcggaca gcgggctgcc atcgggttcc agcacgataa
    28441 aggtatccat cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga acacttaaat
    28501 tgcggcggtc taattcggca cagacggcgg caaacagata ggggcggtcc gggctccaga
    28561 taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag caataccagc ggtttgctta
    28621 aatcatgctg taataaatgg cgggcatgcc aggccagttg atttgggcta tggcggacaa
    28681 aatagttagc acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg atgttatcca
    28741 tgcgcagtag tgccagtgcc tggagttggt gatggcgaac ccgttcgcgc atatccggcg
    28801 tgttttgcat tccgcgtcgt agctgctttt cggtggcaaa atagagttcg cgcaacagac
    28861 tttgcttcca gctattccac agcgtttcgt tggtggcgca aatgtcagcc actgtcaggc
    28921 ataccagata gcgcagacga ttttccgttt gaacttcttc ggcaaactgc ttgatgactt
    28981 cagggtcctg aatatcgcgg cgttgtgctg tcaccgacat cagcaagtgc tggcgaacca
    29041 gccaggcaac cagctgtgtt tcgcgtgagt tcagcccgtg gagttctgca aaatgcacta
    29101 catcctgagc accgagaatg gagtggtcgc cgccgcgtcc tttggcgata tcgtgaaaca
    29161 gcgcggcgat gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc acacacaacg
    29221 gatggcgctg gcgcgtttct tcactggcaa aactctccag tttcagcatc acgcggatag
    29281 tatgttcatc caccgtgtag gcgtggaaca gatcaaactg catctgcccg acgatatgcg
    29341 accattgcgg catatacgcg ccgagcacgc tatggcgatg cattggcaat agcccgcgcc
    29401 gcaccgctcc ggggtgacgc agaatgctca aaaacagttt tcgtgcttgc ggaatattac
    29461 acagcggttg ttgcagatgg cgacgggcat ggcgtaactg gcgcagcgtg gtggagtaaa
    29521 taccggtgat cgcactgttg cgcaccatgg tgtagaacat acgcaagatg gcttccggct
    29581 ggcgcataaa tagtgtttca tcacgcaggt cgattagcgt accgcgtagc tgaaactcat
    29641 cgtcgagtgg acgtggtttt tcgtcggcgg gaagggcgag gatggcttca tcgaacagtt
    29701 gcagcagcat ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa tccttcatca
    29761 tccgctcgac cggttcgttg ccttcaccac tgtaattcag acgctgagcg acgctaagtt
    29821 ggcgatcgaa taacagacga ttatcgtaac ggctgacgac cagatgcagg gcaaagcgaa
    29881 tacgccacag tatatgcaga cattcgttta attccgcccg ctccgctgag gttaagaagc
    29941 caaacccgac catttcatcc agcgatgttg cgccaaaatg acggcgggcc acccattgca
    30001 gagtgtgaat atcgcgcaag ccgccagggc tacttttgat gtctggttca aggttgtagc
    30061 tggtgccatg gtaacgctga tggcgctggt tctgttcttc aactttcgct gcgtagaact
    30121 tgtcggaagg ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg aagagcgcaa
    30181 catcgccaat taataagcgg gattcgatta aattggtggc gacggttaaa tccgataacc
    30241 cttccaacat gcactcttca agcgtgcgca cactatgacc gacttccagc tttacatccc
    30301 agagcagcgt taacagctcg cccacttttt gcgcctgatc gtccgggagc tttttacggc
    30361 ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc gccacgaccg tagccaccga
    30421 cggcaaccaa tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat aatcgttgca
    30481 ggagctggtc tatgaactcg gtacgtgcct caatcaactg ttctgcagag atcccattgt
    30541 caaaggcatc acccagccaa cgctggaaag tatcgatatg ggcttttatc ccacagacgg
    30601 ttaattcatc acggggccag gcgcatggat tttgcggttg accgggcagg gtggagagag
    30661 cggtgtttgc gtactgttct ggaagggtat tcattgtgcg ccacccataa gattaaatta
    30721 tcgcattaaa aaagccggca ttcgccggct tcatcttatt cgtcgtgcga gattatcgcc
    30781 gggatggtgt catccttgcg tagcgtcaaa atttcgcagc cgttatcagt caccacaata
    30841 gtatgctcat attgtgcaga caagctgcga tctttggttt ttaccgtcca gccatctttc
    30901 atggtgcgga tctctttttt acccgcgttg accattggct caatggtgaa cgtcatcccc
    30961 ggtttcagca cgacgttggt ttcacgggag tcatagtgca gcacctgcgg ttcttcatgg
    31021 aagccgcgac caataccgtg tccgcaatat tcacgaacga cggagaagcc ttctgcttcg
    31081 acaaatttct gaatcgccgc accgatttcg cgcagattaa tgcctggttt taccatgcgt
    31141 agcgccaggt acaggctttc ttgcgtgatg cggcacaggc gttcgcccat gatggtcggc
    31201 ttaccgacga taaacatttt cgaggtatcg ccgtggaaac catctttgat tacggtgaca
    31261 tcaatgttaa cgatatcgcc atctttcagc agcttagcat cgtccgggat accgtggcac
    31321 accacttcat taatagagat gcaaacggat ttcggatagc cgtgatagcc gaggcaggca
    31381 gaaaccgcgt gttgttcatt aacaatgtaa tcattacaga tgcgatccag ctcgccggtg
    31441 ctgacgcccg gtttaacata cggttcgatc atctccagca cttcggcagc cagtcggcca
    31501 gcgacgcgca ttttttcgat atcttctggg gtcttgattg agatagccat taattctgtc
    31561 catcagcgtc ggtgataccg acaatatata tgtaagtgcc gtcaatggta tcacacccgg
    31621 gcaaattgag aatcattctg aatttcgcca aacgtgccac tgaaggtttt ctataataga
    31681 aaattcgacg tctgatgctg tacacagcgc caacaattat tggtgtccac gacgtatttg
    31741 tggtataaag cgcgccggac ttccgatcca tttcgtatac acagactgga cggaagcgac
    31801 aatctcactt tgtgtaacaa cacacacgta tcggcacata ttccggggtg ccctttgggg
    31861 tcggtaatat gggatacgtg gaggcataac cccaactttt atatagaggt tttaatcatg
    31921 gcaactgttt ccatgcgcga catgctcaag gctggtgttc acttcggtca ccagacccgt
    31981 tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt tcacatcatc
    32041 aaccttgaga aaactgtacc gatgttcaac gaagctctgg ctgaactgaa caagattgtt
    32101 tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga agcggtgaaa
    32161 gacgctgctc tgagctgcga ccagttcttc gtgaaccatc gctggctggg cggtatgctg
    32221 actaactgga aaaccgttcg tcagtccatc aaacgtctga aagacctgga aactcagtct
    32281 caggacggta ctttcgacaa gctgaccaag aaagaagcgc tgatgcgcac tcgtgagctg
    32341 gagaaactgg aaaacagcct gggcggtatc aaagacatgg gcggtctgcc ggacgctctg
    32401 tttgtaatcg atgctgacca cgaacacatt gctatcaaag aagcaaacaa cttgggtatt
    32461 ccggtatttg ctatcgttga taccaactct gatccggacg gtgttgactt cgttatcccg
    32521 ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc tgcaaccgta
    32581 cgtgaaggcc gttctcagga tctggcttcc caggcggaag aaagcttcgt agaagctgag
    32641 taataaggct tgataactcc cccaaaatag ttcgagttgc agaaaggcgg caagctcgag
    32701 aattcccggg agcttacatc agtaagtgac cgggatgagc gagcgaagac aacgcatctg
    32761 cggcgcgaaa tatgaagggg gagagccctt atagaccagg tagtacacgt ttggttaggg
    32821 ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc
    32881 acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg
    32941 agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca
    33001 tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag
    33061 gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc
    33121 tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag
    33181 acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac
    33241 agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc
    33301 gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggcg
    33361 ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg
    33421 ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag
    33481 aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga
    33541 aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt
    33601 tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga
    33661 ctggcttcat ccgtttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag
    33721 cagaagttgc tgcgatgtcc aagcagtctt aattatcaaa aaggagccgc ctgagggcgg
    33781 cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg
    33841 acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat
    33901 ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct
    33961 gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgtatgg ctcaggaaat
    34021 caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt
    34081 ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat
    34141 gctggcgacc gtaatgaacg gcttggcaat gcgtgatgca ctgcaccgcg cctatgtgaa
    34201 cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga
    34261 agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc
    34321 gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt
    34381 gctgaaagca accaaagttg acggcgtgtt taccgctgat ccggcgaaag atccaaccgc
    34441 aaccatgtac gagcaattga cttacagcga agtgctggaa aaagagctga aagtcatgga
    34501 cctggcggcc ttcacgctgg ctcgtgacca taaattaccg attcgtgttt tcaatatgaa
    34561 caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga
    34621 ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggcgtaag
    34681 tctggttata aggcgttatt gttgcaggca gtttggtcac ggccagcgcg cagcaaccgg
    34741 agcgtacaaa agtacgtgag gatggcgagc actgctcggg gccaaaatgg caaataaaat
    34801 agcctaataa tccagacgat tacccgtaat atgtttaatc agggctatac ttagcacact
    34861 tccactgtgt gtgactgtct ggtctgactg agacaagttt tcaaggattc gtaacgtgat
    34921 tagcgatatc agaaaagatg ctgaagtacg catggacaaa tgcgtagaag cgttcaaaac
    34981 ccaaatcagc aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg gcattgtcgt
    35041 ggaatattac ggcacgccga cgccgctgcg tcagctggca agcgtaacgg tagaagattc
    35101 ccgtactctg aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg aaaaagcgat
    35161 tatggcctcc gatctcggcc tgaacccgaa ctctgcgggt agcgacatcc gtgttccgct
    35221 gccgccgctg acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg gtgaagcaga
    35281 acaagcgcgt gttgcagtac gtaatgtgcg tcgtgacgcg aacgacaaag tgaaagcact
    35341 gttgaaagat aaagagatca gcgaagacga cgatcgccgt tctcaggacg atgtacagaa
    35401 actgactgat gctgcaatca agaaaattga agcggcgctg gcagacaaag aagcagaact
    35461 gatgcagttc tgatttcttg aacgacaaaa acgccgctca gtagatcctt gcggatcggc
    35521 tggcggcgtt ttgcttttta ttctgtctca actctggatg tttcatgaag caactcacca
    35581 ttctgggctc gaccggctcg attggttgca gcacgctgga cgtggtgcgc cataatcccg
    35641 aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg gtagaacagt
    35701 gcctggaatt ctctccccgc tatgccgtaa tggacgatga agcgagtgcg aaacttctta
    35761 aaacgatgct acagcaacag gggagccgca ccgaagtctt aagtgggcaa caagccgctt
    35821 gcgatatggc agcgcttgag gaggttgatc aggtgatggc agccattgtt ggtgctgctg
    35881 ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac cattttgctg gccaataaag
    35941 aatcactggt tacctgcgga cgtctgttta tggacgccgt aaagcagagc aaagcgcaat
    36001 tgttaccggt cgatagcgaa cataacgcta tttttcagag tttaccgcaa cctatccagc
    36061 ataatctggg atacgctgac cttgagcaaa atggcgtggt gtccatttta cttaccgggt
    36121 ctggtggccc tttccgtgag acgccattgc gcgatttggc aacaatgacg cccgatcaag
    36181 cttgccgtca tccgaactgg tcgatgggac gtaaaatttc tgtcgattcg gctaccatga
    36241 tgaacaaagg tctggaatac attgaagcgc gttggctgtt taacgccagc gccagccaga
    36301 tggaagtgct gattcacccg cagtcagtga ttcactcaat ggtgcgctat caggacggca
    36361 gtgttctggc gcagctgggg gaaccggata tgcgtacgcc aattgcccac accatggcat
    36421 ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta agtgcgttga
    36481 catttgccgc accggattat gagcgttatc catgcctgaa actggcgatg gaggcgttcg
    36541 aacaaggcca ggcagcgacg acagcattga atgccgcaaa cgaaatcacc gttgctgctt
    36601 ttcttgcgca acaaatccgc tttacggata tcgctgcgtt gaatttatct gtactggaaa
    36661 aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat gcgaacgcga
    36721 gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc ggctacagag
    36781 agtcgcgcta tttgttagcg tagggcttca atgatatagt ctgcgccatc tgatcgtaag
    36841 tagttggctt tataaggtca ggtatgccgt ggttttacac ggcttttttt tgtataggct
    36901 tcagtattcc tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga tgttgtctgc
    36961 tactcaacca cttagcgaaa aattgccagc gcatggctgc cgtcatgttg cgatcattat
    37021 ggacggcaat ggtcgctggg caaaaaagca agggaagatt cgtgcctttg ggcataaagc
    37081 cggggcaaaa tccgtccgcc gggctgtctc ttttgcggcc aacaacggta ttgaagcgtt
    37141 aacgctgtat gcctttagta gtgaaaactg gaaccgacca gcgcaggaag tcagtgcgtt
    37201 aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc gacataacgt
    37261 gcgtctgcgt attattggcg ataccagtcg ttttaactcg cgtttgcaag aacgtattcg
    37321 taaatctgaa gcgctaacag ccggaaatac cggtctgacg ctgaatattg cggcgaacta
    37381 cggtggacgt tgggatatag tccagggagt caggcaactg gctgaaaagg tgcagcaagg
    37441 aaacctgcaa ccagatcaga tagatgaaga gatgttaaac cagcatgtct gtatgcatga
    37501 actggcccct gtagatttag taattaggac tgggggagag catcgcatta gtaacttttt
    37561 gctttggcaa attgcctatg ccgaacttta ctttacagat gttctctggc ccgatttcga
    37621 tgaacaagac tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc gtttcggcgg
    37681 caccgagccc ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt atcgcctgat
    37741 atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc cgccggtggg
    37801 gttcgccatt gtaacgctgg tggtctgcat gctggcagcg tgggaatggg gacagcttag
    37861 cggttttacc actcgttcgc agcgagtatg gttggcggtg ttatgcgggt tattgttggc
    37921 gctgatgctt tttctgttgc cggaatatca ccgaaatatt catcaaccgc tggttgaaat
    37981 ctcactttgg gcttcgttgg gttggtggat tgtcgcgcta ttgctggtgc tgttttaccc
    38041 aggttccgca gcaatctggc gtaactctaa aacattgcgc cttatttttg gcgtgctaac
    38101 cattgttccc ttcttctggg gcatgctggc gttacgggcc tggcactatg acgagaatca
    38161 ttacagtggc gcaatatggc tgctctatgt catgatcctg gtatggtgcg ctgactccgg
    38221 cgcatatatg tttggcaaat tgtttggtaa acataagctg gcaccgaagg tttctccggg
    38281 taaaacctgg caaggcttta taggtggact cgctactgca gcggtaatct catggggtta
    38341 tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct ctattgtcgc
    38401 agcgttagcc tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg aagcaggaat
    38461 taaggacagc ggtcatttaa ttccaggaca cggtggtatt ttagatcgta ttgatagcct
    38521 gacggctgcg gtaccggtct ttgcttgctt gttgttactg gtattcagga cgctttaacg
    38581 gaaggtaata tgctgagttt tctctgggat ttggcttcgt tcatcgttgc actgggtgta
    38641 cttatcaccg tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg tgttcgcgtt
    38701 gagcgtttct caatagggtt tggtaaggcg ctctggcggc gaactgataa gctcggcacc
    38761 gaatatgtta tcgccctgat cccgttgggc ggttatgtca aaatgctgga tgagcgcgca
    38821 gaaccggtcg ttccggaact ccgccaccat gccttcaata ataaatctgt cggccaacga
    38881 gtggcgatta ttgccgcagg tccggttgca aacttcattt ttgctatctt tgcctactgg
    38941 ctggttttta ttattggtgt gcctggcgta cgtccggtgg ttggcgaaat agcagccaat
    39001 tcgatagctg cggaagcaca aattgcacca ggtacggaac taaaagccgt agatggtatc
    39061 gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg ataaaattgg cgatgaaagc
    39121 accaccatta cagtagcgcc atttggcagc gaccaacggc gggatgtaaa gctcgattta
    39181 cgtcactggg cgtttgagcc tgataaagaa gatccggtat cttcgctggg gattcgtcct
    39241 cgtgggccgc aaattgaacc tgtactggaa aatgtgcagc caaactcggc ggcaagcaag
    39301 gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg gtcagccctt aacgcagtgg
    39361 gtgacctttg tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt agaaatcgaa
    39421 aggcagggga gtcccttgtc tttgacatta atcccggaga gtaaaccggg taatggtaaa
    39481 gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt tgccagatga gtataaagtt
    39541 gtacgccagt atgggccgtt caacgccatc gtcgaagcca cggacaaaac gtggcagctg
    39601 atgaagctga cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa actgaacaac
    39661 ctcagtgggc cgatttctat cgccaagggg gctgggatga cagcggaact cggggtagtt
    39721 tattacctgc cgtttcttgc gcttattagc gtgaacttag ggataattaa cctgtttccg
    39781 ttgcccgtac ttgacggggg gcatctgctg ttccttgcga tcgaaaagat caagggcgga
    39841 ccggtatccg agcgggttca agacttttgt tatcgcattg gctcgattct gctggtgctg
    39901 ttaatggggc ttgcactttt caatgatttc tctcggttat gagagttagt taggaagaac
    39961 gcataataac gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt agcagcgcca
    40021 ccgtatacgg tgctgaaggg ttcgtagtga aagatattca tttcgaaggc cttcagcgtg
    40081 tcgccgttgg tgcggccctc ctcagtatgc cggtgcgtac aggcgacacg gttaatgatg
    40141 aagatatcag taataccatt cgcgctctgt ttgctaccgg caactttgag gatgttcgcg
    40201 tccttcgtga tggtgatacc cttctggttc aggtaaaaga acgtccgacc attgccagca
    40261 ttactttctc cggtaacaaa tcggtgaaag atgacatgct aaagcaaaac ctcgaggctt
    40321 ctggtgtgcg tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc gagaaaggtc
    40381 tggaagactt ctactacagc gtcggtaaat atagcgccag cgttaaagct gtcgtgaccc
    40441 cgctgccgcg caaccgtgtt gacctaaaac tggtgttccg ggaaggtgtg tcagctgaaa
    40501 tccagcaaat taacattgtt ggtaaccatg ctttcaccac cgacgaactg atctctcatt
    40561 tccaactgcg tgacgaagtg ccgtggtgga atgtggtagg cgatcgtaaa taccagaaac
    40621 agaaactggc gggcgacctt gaaaccctgc gcagctacta tctggaccgc ggttatgccc
    40681 gtttcaacat cgactctacc caggtcagtc tgacgccaga taaaaaaggt atttacgtca
    40741 cggtgaacat caccgaaggc gatcagtaca agctttctgg cgttgaagtg agcggcaacc
    40801 ttgccgggca ctccgctgaa attgagcagc tgactaagat cgagccgggc gagctgtata
    40861 acggcactaa agtgaccaaa atggaagatg acattaaaaa gcttctcggt cgctatggtt
    40921 atgcctatcc gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa accgttaaat
    40981 tacgcgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt tttgaaggta
    41041 acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa ggtgcatggt
    41101 tggggagcga tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc ttctttgaaa
    41161 ctgtcgatac tgatacccaa cgtgttccgg gtagcccgga ccaggttgat gtagtctaca
    41221 aggtgaaaga acgtaacact ggtagcttca acttcgggat tggttacggt actgaaagtg
    41281 gcgtgagctt ccaggctggc gtgcaacagg ataactggtt aggtacaggt tatgctgttg
    41341 gtatcaacgg gaccaaaaac gattaccaga cctatgctga actgtcggta accaacccgt
    41401 acttcaccgt agatggcgta agcctcggtg gtcgtctctt ctataatgac ttccaggcag
    41461 atgacgccga cctgtccgac tataccaaca agagttatgg tacagacgtg acgttgggct
    41521 tcccgattaa cgaatataac tcgctgcgtg caggtctggg ttatgtacat aactccctgt
    41581 ccaacatgca gcctcaggtt gcgatgtggc gttatctgta ctctatgggt gaacatccga
    41641 gcacttctga tcaggataac agcttcaaaa cggacgactt cacgttcaac tatggttgga
    41701 cctataacaa gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc aacctgaccg
    41761 gtaaagtgac cattcctgga tcggataacg aatactacaa agtgacgtta gacacggcga
    41821 cttatgtgcc gatcgatgac gatcacaaat gggttgttct gggtcgtacc cgctggggtt
    41881 atggtgatgg tttaggcggc aaagagatgc cgttctacga gaacttctat gccggtggtt
    41941 ccagcaccgt gcgtggcttc cagtccaata ccattggtcc gaaagcagtt tacttcccgc
    42001 atcaggccag taattatgat ccggactatg attacgaatg tgcgactcag gacggcgcga
    42061 aagacctgtg taaatcggat gatgctgtag gcggtaacgc catggcggtt gccagcctcg
    42121 agttcatcac cccgacgccg tttattagcg ataagtatgc taactcggtt cgtacttcat
    42181 tcttctggga tatgggtacc gtttgggata caaactggga ttccagccaa tattctggat
    42241 atccggacta tagtgatcca agcaatatcc gtatgtctgc gggtatcgca ttacaatgga
    42301 tgtccccatt ggggccgttg gtgttctcct acgcccagcc gttcaaaaag tacgatggag
    42361 acaaggcaga acagttccag tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg
    42421 aatgtagtgg tagtgtagcg atgactttag gcgatcaata taagatcgcc gggccacgca
    42481 aagaactgca ccctccggtg caaatgggat ggtaaggagt ttattgtgaa aaagtggtta
    42541 ttagctgcag gtcttggttt agcactggca acttctgctc aggcggctga caaaattgca
    42601 atcgtcaata tgggcagcct gttccagcag gtagcgcaga aaaccggtgt ttctaacacg
    42661 ctggaaaatg agttcaaagg ccgtgccagc gaactgcagc gtatggaaac cgatctgcag
    42721 gctaaaatga aaaaactgca gtccatgaaa gcgggcagcg atcgcaccaa actggaaaaa
    42781 gacgtgatgg ctcagcgcca gacttttgct cagaaagcgc aggcttttga gcaggatcgc
    42841 gcacgtcgtt ccaacgaaga acgcggcaaa ctggttactc gtatccagac tgctgtgaaa
    42901 tccgttgcca acagccagga tatcgatctg gttgttgatg caaacgctgt tgcttacaac
    42961 agcagcgatg taaaagacat cactgccgac gtactgaaac aggttaaata agtaatgcct
    43021 tcaattcgac tggctgattt agcgcagcag ttggatgcag aactacacgg tgatggcgat
    43081 atcgtcatca ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat tacgttcatg
    43141 gttaacccaa aataccgtga gcatttaggc ttgtgccaag cgtccgcggt tgtcatgacc
    43201 caggacgatc ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc ctacctgact
    43261 tacgcgcgca tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa cattgcaccc
    43321 agtgcggtga tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg cgctaacgcg
    43381 gtgattgagt ccggcgttga actgggcgat aacgtgatta tcggtgccgg ttgcttcgta
    43441 ggtaaaaaca gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac catttaccat
    43501 gagatccaga tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg cgcagacggc
    43561 tttggttatg ccaacgatcg tggtaactgg gtgaagatcc cacagattgg tcgcgtaatt
    43621 attggcgatc gagtggagat cggtgcctgc acaaccatcg atcgcggcgc gctggatgac
    43681 actgttattg gcaatggcgt gatcattgat aaccagtgcc agattgcaca taacgtcgtg
    43741 attggcgaca atacggcggt tgccggtggc gtcattatgg cgggcagcct gaaaattggt
    43801 cgttactgca tgatcggcgg agccagcgta atcaacgggc atatggaaat atgcgacaaa
    43861 gtgacggtta cgggcatggg tatggtgatg cgtcccatca ctgaaccagg cgtctattcc
    43921 tcaggcattc cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact ggtgatgaac
    43981 attgatgaca tgagcaagcg tctgaaatcg cttgagcgca aggttaatca acaagactaa
    44041 cgttccatct tttgttcgcc aaactttacg gcctgtctca ttcttacgat tgcggcaggc
    44101 cgtgttatta ttgtcgtttc ttatattttg acaggaagag tatcttgact actaacactc
    44161 atactctgca gattgaagag attttagaac ttctgccgca ccgtttcccg ttcttactgg
    44221 tggatcgcgt gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa aatgtctctg
    44281 tcaatgagcc attcttccag ggccatttcc ctggaaaacc gattttcccg ggtgtgctga
    44341 ttctggaagc aatggcacag gcaacaggta ttctggcgtt taaaagcgta ggaaaactgg
    44401 aaccgggtga gctgtactac ttcgctggta ttgacgaagc gcgcttcaag cgcccggtcg
    44461 tgcctggcga tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc ggcctgaccc
    44521 gttttaaagg ggttgctctg gtcgatggta aagtagtttg cgaagcaacg atgatgtgtg
    44581 ctcgtagccg ggaggcctga tacgtgattg ataaatccgc ctttgtgcat ccaaccgcca
    44641 ttgtggaaga gggcgcgtca attggcgcga acgcacacat tggtcctttt tgtatcgttg
    44701 gaccccatgt cgaaattggt gagggtaccg tactgaaatc tcacgttgtc gtgaatggtc
    44761 atactaaaat tggccgcgat aatgagattt atcagtttgc ctccatcggc gaagttaacc
    44821 aggatttgaa atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt aaccgcattc
    44881 gcgaaagcgt caccattcat cgtggcacag tccagggcgg tggattgacg aaggtgggca
    44941 gcgacaactt actgatgatc aacgcgcaca ttgcgcacga ttgtacggta ggtaaccgct
    45001 gtattctcgc caacaacgca acgctggcgg gtcacgtatc ggttgacgac ttcgcgatca
    45061 tcggcggcat gaccgcagtc catcagttct gcatcattgg tgcgcatgtg atggttggcg
    45121 gctgctccgg tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt aaccacgcaa
    45181 cgccgttcgg tgtcaatatc gaagggctga agcgccgcgg attcagccgt gaggcgatta
    45241 ccgctatccg caatgcgtat aagctaattt atcgtagcgg taaaacgctc gatgaagtga
    45301 aaccggaaat tgctgaactg gcggaaacat atccggaagt gaaagccttt accgatttct
    45361 ttgcacgctc aacgcgcggt ctgattcgtt aatgactgac cagcgtccat taacgattgc
    45421 cctggtcgcc ggagaaatct ccggcgatat cctgggggcc ggtttaatcc gcgctctgaa
    45481 agaacgtgtg cccaacgccc gctttgttgg tgttgccggg ccacgaatgc aggctgaagg
    45541 ctgcgaagcc tggtacgaaa tggaagaact ggcggtgatg ggcattgttg aagtgctcgg
    45601 tcgtctgcgt cgcttactgc atattcgtgc cgatctgaca aagcgttttg gcgaactgaa
    45661 gccagatgtt tttgttggta ttgatgcgcc cgacttcaat attactcttg aaggtaacct
    45721 caaaaagcag ggtatcaaaa ccattcatta cgtcagtcca tccgtctggg cgtggcgaca
    45781 gaaacgcgtt ttcaaaatag gcagagccac cgatctggtg ctcgcatttc tgcctttcga
    45841 aaaagcgttt tatgacaaat acaatgtacc gtgccgcttt atcggtcata ccatggctga
    45901 tgccatgccg ttagatccag ataaaaatgg tgcccgtgat gtgctgggga tcccttacga
    45961 tgcccactgt ctggcattgt tgccgggcag ccgtggtgca gaagtcgaaa tgcttagtgc
    46021 cgatttcttg aaaactgccc agcttttgcg ccagacatat ccggatctcg aaatcgtggt
    46081 gccgctggtg aatgccaaac gccgcgagca gtttgaacgc atcaaagctg aagtcgcgcc
    46141 agacctgtcg gttcatttgc tggatggaat gggccgtgag gcgatggtcg ccagcgatgc
    46201 ggcactactg gcgtcgggga cggcagccct ggagtgtatg ctggcgaaat gcccgatggt
    46261 ggtgggatat cgcatgaagc cttttacctt ctggttggcg aagcggctgg tgaaaactga
    46321 ttatgtctcg ctgccaaatc tgctggcggg cagagagtta gtcaaagagt tattgcagga
    46381 agagtgtgag ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga acgggaaaac
    46441 cagccacgcg atgcacgata ccttccgtga actgcatcag cagatccgct gcaatgccga
    46501 tgagcaggcg gcacaagccg ttctggagtt agcacaatga tcgaatttgt ttatccgcac
    46561 acgcagctgg ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg cgcggtcgtc
    46621 accgctgcgg tgatccttga cccggcgcgc ccgattgccg ggctgaatga ttccaaaaag
    46681 ctgagcgaaa aacgccgtct ggcgctctat gaagagatca aagagaaagc gttgagctgg
    46741 agtctgggcc gcgcggaacc ccacgaaatc gacgagctga acattcttca tgcgaccatg
    46801 ctggcgatgc agcgtgccgt cgctgggctg catattgcgc cggaatatgt gttgattgat
    46861 ggtaaccgct gcccgaaatt accgatgcct gcgatggctg tggtgaaagg ggatagccgc
    46921 gtaccagaaa tcagtgccgc gtctatccta gcgaaagtga cgcgtgacgc cgaaatggcg
    46981 gcgctggata ttgttttccc gcaatatggt tttgcccaac ataaagggta tccaaccgct
    47041 tttcatctgg aaaaactggc tgaatatggc gcgactgaac atcatcgtcg cagctttggg
    47101 cctgtcaaac gcgcactggg acttgcgtcc tgattcttgt gtcgagatta agtaaaccgg
    47161 aatctgaaga tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg
    47221 atcgatggct tggccaaaac cgcaccgctg gtaaaaaagg cggcggcgtt gggtatgcca
    47281 gcactggcga tcaccgattt caccaacctt tgcggtctgg tgaagttcta cggagcggga
    47341 catggcgcag ggattaagcc tatcgtcggg gcagatttta atgtccagtg cgacctgctg
    47401 ggtgatgagt taacccacct gacggtactg gcggcgaaca ataccggcta tcagaatctg
    47461 gcgttgctga tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc gatcatcgat
    47521 cgcgactggc ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg gcgcatgggc
    47581 gacgtcggac gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg tgtcgcgttt
    47641 tatgaagaac acttcccgga tcgctatttt ctagagctga tccgcaccgg caggccggat
    47701 gaagaaagct atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt gcccgtcgtg
    47761 gcgactaacg atgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc
    47821 gcgatccacg acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag
    47881 caatatatgc gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc agaagccctt
    47941 gccaacaccg ttgagatcgc caaacgctgt aacgtaactg tgcgtcttgg tgaatacttc
    48001 ctgccgcagt tcccgaccgg ggacatgagc accgaagatt atctggtcaa gcgtgcaaaa
    48061 gaaggcctgg aagagcgtct ggcattttta ttccctgacg aggaagaacg tcttaaacgc
    48121 cgcccggaat atgacgaacg tctggagact gaacttcagg ttatcaacca gatgggtttc
    48181 ccgggctact tcctcatcgt tatggaattt atccagtggt cgaaagataa cggcgtaccg
    48241 gtaggaccag gccgtggctc cggtgcgggt tcactggtgg cctacgcgct gaaaatcacc
    48301 gacctcgatc cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc
    48361 tccatgcctg acttcgacgt tgacttctgt atggagaaac gcgatcaggt tatcgaacac
    48421 gtggcggaca tgtacggtcg tgatgcggta tcgcagatta tcaccttcgg tacgatggcg
    48481 gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc atccgtacgg ctttgtcgac
    48541 cgtatctcga aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc
    48601 gagccgcagc tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct gatcgacatg
    48661 gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc
    48721 gcgccgacca aaattaccga ttttgcgccg ctttattgcg atgaagaggg caaacatccg
    48781 gtcacccagt ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt
    48841 ggtttgcgta cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg
    48901 aagaatggcg agccaccgct ggatatcgcc gcgatcccgc tggatgacaa gaaaagcttc
    48961 gacatgctgc aacgctcgga aaccacggcg gtattccagc ttgaatcgcg cggcatgaag
    49021 gacctgatca agcgtctgca acctgactgc ttcgaagata tgatcgcact ggtggcactg
    49081 ttccgccctg gtccgttgca gtcagggatg gtggataact ttatcgaccg taaacatggt
    49141 cgtgaagaga tctcctatcc ggacgtgcag tggcagcatg aaagcctgaa atcggtactg
    49201 gagccaacct acggcattat cttgtatcag gaacaggtca tgcagattgc ccaggtgctt
    49261 tctggttata ccctcggtgg cgcggatatg ctgcgccgtg cgatgggtaa gaaaaagccg
    49321 gaagagatgg ctaagcagcg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac
    49381 gccgaactgg cgatgaaaat cttcgacctg gtggagaaat tcgcgggtta cggatttaac
    49441 aaatcgcact ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcgcac
    49501 tatccggcgg agtttatggc ggcggtaatg accgccgata tggacaacac cgagaaggtg
    49561 gtgggcctgg tggatgagtg ctggcggatg gggctgaaaa tcctgccacc agatataaac
    49621 tccggtcttt accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg tattggcgcc
    49681 atcaaagggg taggtgaagg tccgattgag gccatcatcg aagcccgtaa taaaggcggc
    49741 tacttccgcg aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt aaaccggcgg
    49801 gtgctggaaa aactgatcat gtccggggcg tttgaccgtc ttgggccaca ccgcgcggcg
    49861 ctgatgaact cgctgggtga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct
    49921 atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat tgaacaatcc
    49981 tacgccagct gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta
    50041 ggcctgtacc tgaccggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc
    50101 ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacagctgcg
    50161 gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc
    50221 acgctggatg accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa
    50281 taccagcaat tgctggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat
    50341 gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg
    50401 gaaaaatatg ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt
    50461 ttaaaccgac tccgtcagtc tctggaaccc caccgctctg ggacaattcc agtacatctc
    50521 tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct
    50581 ccgagcgatc gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg
    50641 gagtttgact aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt
    50701 gcagagctgg aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg
    50761 gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacgcgt
    50821 aaaatcttcg ccgatctcgg tgcatggcag attgcgcaac tggcacgcca tccgcagcgt
    50881 ccttataccc tagattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac
    50941 cgtgcgtatg cagacgataa agctatcgtc ggtggtatcg cccgtctcga tggtcgtccg
    51001 gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt
    51061 ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc
    51121 tttaagatgc ctatcatcac ctttatcgac accccggggg cttatcctgg cgtgggcgca
    51181 gaagagcgtg gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgccttggc
    51241 gtaccggtag tttgtacggt tatcggtgaa ggtggttctg gcggtgcgct ggcgattggc
    51301 gtgggcgata aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt
    51361 tgtgcgtcca ttctgtggaa gagcgccgac aaagcgccgc tggcggctga agcgatgggt
    51421 atcatcgctc cgcgtctgaa agagctgaaa ctgatcgact ccatcatccc ggaaccgctg
    51481 ggcggcgctc accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgctggcg
    51541 gatctggccg atctcgatgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc
    51601 ctgatgagct acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc
    51661 ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac
    51721 acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca
    51781 aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca caaaacagcg
    51841 ttgatttgct gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg
    51901 atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat ctcccgcttt
    51961 atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc
    52021 tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt
    52081 acaccaacga atatcttgat aacattacgc cgccgttcac gaaagccttg tttacctacg
    52141 tcaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc gcatatcaaa
    52201 aaagcccggt tggctgtctg ttttatgatt ttttcggcgg gaataccctt aaggctgatg
    52261 tctctatttc ggtcaccgag cttggttcgt tgcttgacca caccgggcca cacctggaag
    52321 cggaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt accaacggga
    52381 catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt acgctgttga
    52441 tcgaccgcaa ctgccataaa tcactggcgc atctgttgat gatgaacgat gtagtgccag
    52501 tctggctgaa accgacgcgt aatgcgttgg ggattctggg ggggatcccg cgccgtgaat
    52561 ttactcgcga cagcatcgaa gagaaagtcg ctgccactac gcaagcacaa tggccggttc
    52621 atgcggtgat caccaactcc acctatgatg gtttgctcta caacaccgac tggatcaaac
    52681 agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtat actcattttc
    52741 atccaatcta ccagggtaaa agtggtatga gcggcgagcg tgttgcggga aaagttttct
    52801 tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttca ctgatccaca
    52861 ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct
    52921 cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg cgtggtaatc
    52981 cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc aaagaggtcc
    53041 agcggctgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg
    53101 atgaagccga atgctggccc gttgcgcctg gcgaacaatg gcacggcttt aacgatgcgg
    53161 atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg gggatggacg
    53221 aacagggcaa tatgagtgag gaggggatcc cggcggcgct ggtagcaaaa ttcctcgacg
    53281 aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc tttagtattg
    53341 gcatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt aagcgctctt
    53401 acgatctcaa cctgcggatc aaaaatatgc tgcccgatct ctatgctgaa gatcccgatt
    53461 tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg attcgtaaac
    53521 acgatctttc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg atcatgacgc
    53581 cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg ctggaacaac
    53641 tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta ccgctgttga
    53701 tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta ctgatgcttt
    53761 gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg aaacaggacg
    53821 aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt
    53881 ctgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt
    53941 tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct actgcgatat
    54001 tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga
    54061 tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc cgccggagcg
    54121 ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgacatcga
    54181 tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc
    54241 atacacgcaa aaacgcttca ccttctttaa cgatccggac gggctgccgt tggaactgta
    54301 tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt
    54361 aaaatcgcac catcatgaca ctcacgctca atagaccact tctcacctca cgccagattc
    54421 tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg gtgcagtggc
    54481 ggacggaaaa tccgggcgtc actctgcgcg ctattcatgt gcatcacggt ttaagtgcca
    54541 atgccgatgc ctgggttacg cattgcgaaa acgtctgcca acagtggcag gtgccgctgg
    54601 tggtcgaacg cgtacaactt gcgcaagagg ggctggggat tgaggcccag gcgcggcagg
    54661 cgcgttatca ggcatttgcc cgtaccctat tgcccggcga agtgctggtc accgcgcaac
    54721 atctcgacga tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc ggccctgccg
    54781 ggctttcggc tatggcggaa gtttcggagt ttgctggaac acggcttatc cgcccgttgc
    54841 ttgcccgcac gcggggggaa ctggagcagt gggcattggc gcatggttta cgctggattg
    54901 aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg cgcgtagtgc
    54961 cgttattgca gcagcgttgg ccgcattttg cggaagcaac ggcccgcagc gccacacttt
    55021 gtgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca cactgccaaa
    55081 cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc cgtcgcgcgg
    55141 cgattattcg ccgctggctg gcagggcaga atgcaccgat gccttcccgc gacgcgctgg
    55201 tgcgcatctg gcaggaagtg gcgctggcgc gggaagatgc ttccccctgt ttacgtttgg
    55261 gcgcctttga aatccgccgc tatcagtcgc aactgtggtg gattaaatcc gtcaccgggc
    55321 aaagcgaaac cattgtgctg tggcagacgt ggctacaacc gctggaatta ccggcggggc
    55381 tgggaaccgt acagcttact gcgggaggcg atattcgccc tccgcgtgca gacgaagcgg
    55441 tcagcgtgcg tttcaaagcg ccaggattgc tgcatattgt cgggcgcaac ggcggacgta
    55501 agctgaagaa aatctggcaa gagttaggcg tgccgccgtg gctacgtgac accacgccac
    55561 tgctgtttta tggagaaacg ctgattgcgg cggcaggggt atttgtgacg caagaaggtg
    55621 tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt taagtgaaag
    55681 ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggattc gcttaccacc
    55741 accgtaccga tttcagggtg gctaaagctg gtaattttat ccagacgcag ctcgcgggtt
    55801 tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa atcactggct
    55861 tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag
    55921 gcgagctcaa gattatcgta atcatcacaa ttaattggtt gatacgtatc attcattgac
    55981 ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga
    56041 aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg
    56101 aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata
    56161 tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta
    56221 cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca atatcagcgc
    56281 cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg
    56341 cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg caaccagttt
    56401 ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg tatgatgctg
    56461 cgcttgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg tggtttgtgt
    56521 gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt
    56581 gagctggggc tgccggatga gcgacgaatt aagaaagcct gcacccaggc cgcagaagtg
    56641 gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa tcagagcaaa
    56701 tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact ggcgaaagta
    56761 agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg tgttatctgg
    56821 ttatcggatg ataagaataa tctggaagtt aacttaaccg cctggtaaca accttcatga
    56881 ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc gccattcgtg
    56941 cgcagggcgc tggcgggcag catgttaata agacctcaac ggctattcat ctgcgttttg
    57001 acattcgggc gcccagcctg ccagagtatt acaaagagcg tctgctcgcc gccagccatc
    57061 atttgatcag cagtgatggc gtgattgtca ttaaggcaca ggaataccgc agtcaggaac
    57121 tgaaccgcga agccgctctg gcccggttgg tggcagtgat taaagattta acaaaagaac
    57181 aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg ctggcatcga
    57241 aagcacaaaa atcaagcgtg aaggcgatgc gcggcaaagt acgcagcggt cgggaataaa
    57301 aagaaggaat ggatggtgaa aaaagcgata gtgacagcga tggctgtaat cagcctcttt
    57361 actctgatgg gatgtaataa tcgggccgaa gtcgatacgc tttctccggc gcaggctgcc
    57421 gaactgaaac cgatgccgca aagttggcgc ggcgtgctac cgtgtgccga ttgcgaagga
    57481 atcgaaatct ctctgttcct cgaaaaagac ggaacatggg tgatgaatga gcgttatctc
    57541 ggtgctcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg aaccgctgac
    57601 aagctggtat taaccgacag caaaggtgaa aagtcatatt atcgggcgaa aggcgatgcg
    57661 ctggagatgc tcgatcgtga aggcaatcca attgaatcgc agttcaacta tacgctggaa
    57721 ccggcacaat ccagtttacc tatgacgccg atgaccctgc gaggcatgta tttttatatg
    57781 gctgatgcgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt agcgaataac
    57841 gcagagctgg agcgtagcta cctggctgcg cgcggtcaca gtgaaaaacc ggtgttactg
    57901 tcagtagaag gtcactttac gcttgaggct aatccggata ctggtgcgcc aaccaaagta
    57961 ttagcgcccg atacggcggg taaattttac ccgaataagg attgcagtag tttggggcag
    58021 taaacctgct taaggcagaa acaaacgcaa aattgcctga tgcgctgcgc ttatcaggcc
    58081 tacagcatat aatgcaacat tttgaattag cacgattttg taggccggat aaggcgttta
    58141 cgccgcatcc ggcattttgt gcacgaggct ggctttatca ctgagtcagc ccaacaaagc
    58201 gacccgcctt gatataaatc ccaggcttca ggtgcccaac atactgtaac tgcgtttctg
    58261 gcttaaacgc agagacatca ccaatacgca tatgcatcag atcggcaggg ctaatccagc
    58321 ccgattgcgc cagtgtaagt ggatgcccgg ctttggcaaa tgcatcagtg acaaattccg
    58381 aacaaaacca cgactttttg tctccttcgc acacactgct taactgcgct ttcgccaggt
    58441 cgctgacgca ctgttggcga aaatcctcgg agaacggatt cagtgagcac atctggcgag
    58501 tcaccataaa gggaataaat tcgacaatgt tgcgatagtt ataaccgcta tctttgattt
    58561 tattggcaaa cgcggtgata tctgtggctt gttgcggggt aagatccggg actcgtaaga
    58621 cgaaaagctt atcactatgc ttcatcgctt ttttaaggga aacaatctgg acgccagcgc
    58681 ctgtcgcttc tgcaacgtta ttatcaccca gaaagattgc aacgtgactc acagaggaag
    58741 tgctgaagac gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc
    58801 cgggtttgag atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat
    58861 gctggaattt gacagcccat gtttttgcct cggcatctac tgctgtggct gatgggtctg
    58921 gctggctgat atcaacggta caagccgaaa gtaaaagaaa gctggggaga agcaggcggc
    58981 agtacgcctt tggtttatcc attttataca atccatgtaa aaaagggccc tgaaattcag
    59041 gaccctttct ggcatcagcc tttaatctgt ttcaccagat attcgacgat gtcaccagtc
    59101 ttaattaact gtttctcgcc gttgcgacga tatttatatt cgatatcgtc gttgtcgagg
    59161 ttacggtcgc ccagcacaat agtgtgcgga ataccgatca gttccatatc agcaaacatc
    59221 acgcccggac gctctttgcg gtcatccagc agcacttcga taccttgtgc acgcagttcg
    59281 ctgtacagtt tctcagcaag ctcttgtacg cggaaggatt tgtgcatgtt catcggcaga
    59341 atcgccacct ggaacggcgc gatagcgtca ggccatacga tgccgcgttc gtcgtagttc
    59401 tgctcaatcg ccgcagctac cacacgcgtt accccgatac cgtagcaacc catcgtcagg
    59461 atttggttac ggccatcttc accctgtacg gaggctttca gtgcttcgga gtacttggta
    59521 cccagctgga agatgtgacc aacttcgata ccacgtttga tcagcagcgt accctggcca
    59581 tccgggcttg gatcgccagc caccacgtta cggatatctg caacttccgg ggtagcgaca
    59641 tcgcgatccc agttgatacc gaagtagtgt ttaccatcga tgttagcacc agcagcgaaa
    59701 tcactcatcg ccgcaacggt acggtcaatc accaccggaa tcggcatgtt taccggaccc
    59761 agtgaacccg gaccggcttt aaccacagca cgaatttctt cttcggtcgc gaaagtcagc
    59821 gggcttgcaa cctgcggcag tttttctgct ttaacttcgt tcagctcgtg atcaccgcgc
    59881 accagcagcg caaccagcgg gaagctactg ccttcaaccg ctttaaccag cagagtctta
    59941 accgttttct caatcggcag attgaactgt tcaaccagtt ccgcgatggt tttcgcgttc
    60001 ggcgtatcaa ccagcgtcat ttcctgggta gcagcagcgc gcggttcttt cggcgcgata
    60061 gcttctgcca gttcaatgtt cgctgcatag tcagaggtgt cggagaagac cacatcgtct
    60121 tcaccgctct gcgccagcac ctggaattcg tgagaggcgc tgccgccgat agaaccggtg
    60181 tcggcctgta cggcgcggaa atccagcccc atgcggctga agattttgct gtaggccgca
    60241 tacattgcat cgtaggtttc ctgcagggat tcctgagaag tatggaaaga gtaagcatct
    60301 ttcatcagga attcgcggga acgcatgacg ccgaaacgcg gacgcacttc gtcgcggaac
    60361 ttggtctgga tctgatagaa gttcagcggc agctgtttgt aagagctaag ctcgttacga
    60421 atcaggtcag tgataacttc ttcatgagtt gggccgagta cgaacggacg ctcgccacgg
    60481 tcaacaaaac gcagcagttc cggaccgtac tgttcccaac gaccactctc ttgccacaaa
    60541 tcggctggct gaaccaccgg catcgacacc tcgatcgcac cggcgttgtt catctcttca
    60601 cgcacgatgt tttcgacttt tttcagaacg cgcacgccgg tcggcagcca ggtatataac
    60661 ccggaggcca gcttgcggat catcccggcg cgcagcatca gctgatggct gatcacctcg
    60721 gcgtcggcag gtgtctcctt gagagtggag agcaggtatt ggctagtacg catgttgtta
    60781 cggttccagt tggaaggtag aacaggctca aggcgagcct gggacaaaaa aagtgattta
    60841 gtttaccagt gcaaaagaaa tgtcaaaaga gaagggcgtg aatttaacgc ggttccagcg
    60901 caaagacttc aaaacctgcg tcggtgacgc gccagcgaac gttaaaatca tgtagccaga
    60961 cggcataggt tttgcccgtt tcctcacctt tacgataggc cgggcgcggg tcctgcgcca
    61021 gtacttcgcg gataaacagc gttaactgcg gataacgctt ctccagcgtc aaaagctgct
    61081 tttcgacctc tgcggtaaaa ctcaccgcca tctctgcagc tggcgcactt tgcgcatagc
    61141 tggcactggc atcgggaagc gattcggcaa aggggagata cggtttgata tccactaccg
    61201 gcgtaccatc gaccagatcc agactgccga gcttcagaat cacgctgtct ttatggcaaa
    61261 caacctcttt cagctctacc agcgacatgc caattgggtt agggcggaaa gtagagcgtg
    61321 ttgcgaaaac ccccattctg gcgttaccgc cgaggcgcgg tggacgcaca gtcggacgcc
    61381 agccgccttc catcgtttga tgaaagacga aaaggatcca taaatggctg aatgcttcca
    61441 ggccgcgaac ggcgtcggcc tggttgtagg aagcaatgag atgcagttcg ccgttggcgc
    61501 tttttaccag acctggctgg cgcggaacgg cgaatttttc tttatagggc gagcgaataa
    61561 cgcctatttg ctcgaactgg aaactgctca tttcgccgta atgttaagcg cagagccgat
    61621 acatacagcc tgacgatagc agcccggcgt accgctggtg acttcgcagc tatgcagtaa
    61681 tacagcattg gctttcattt tagaggcatt gatttgcatc tgcttacgtg cggttggaat
    61741 gctcggcgga gagtcctgat tagaggcctg gcaagagtcg ccactgactt caccgagatc
    61801 gcggaacggt ttgccgacta attcttctgc attggtataa attcggaccg gcgtggcgcg
    61861 cggcgctttc ggttttgcag gctccgcttt cggctggggt gcagtgcttt gaacgggttc
    61921 gacaggggat ctgcttaaca tggaacagcc gcttagcatg agtgctacta aacagatcgg
    61981 taaagcacgc atagtatttc ctcaatgtat gatcaaaacg tcaatattga atcaggagct
    62041 tgtaaaaatg acaagacggg caagcgcccg tcctgaatga tattacaaat tgtggaaaca
    62101 gcctaaaaat taccagcctt taacagcgcc gccgttaaac actttgtttg ctgcttcgta
    62161 aacttcatca gactgataag cctggacgaa tttcttcacg ttctcggcgt ctttgttatc
    62221 ttcacgcgtt acgatcaggt ttacgtatgg ggagtcttta tcttcgacaa agataccgtc
    62281 tttcgccgga gtcaggccaa tctggctggc ataggtggta ttgataactg ccagagcgat
    62341 ttgcgcgtcg tccagagaac gcggcagttg cggtgcttcc agttcaacaa ttttcagatt
    62401 ttttgggttc tcaacaacat caagaactgt cggcagcagg ccaacgccat ctttcagttt
    62461 gatcaagccc actttttgca gcagcagcag tgaacgacca agattagttg gatcgtttgg
    62521 cacggcaacc tgcgaaccat cctgcagttc atccagtgat ttgattttct tggagtaacc
    62581 agcaatcgga taaacaaaag tgttgcctac tgcgaccagt ttgtagccac gatctttcag
    62641 ttgctgatca aggtacggtt tatgctggaa ggcgttggcg tcgatatcgc ctttgctcaa
    62701 tgcttcgttt ggcagaacat agtcgttgaa ggttaccagc tcaacgtcca ggccatattt
    62761 gtctttcgca actttctgcg cgacttctgc tacctgctgt tcggcaccaa caatcacgcc
    62821 gactttaatg tggtttggat ctttttcatc ctgaccgcag cctaccagtg ccagtgatcc
    62881 aatcagggct cccactgccg caaaggtttt gaatttgaac gccatacctt attccttctt
    62941 caattattta tgttgtgttg aacgttactt gcgagtgaca gcccggacga tgcggtcgcc
    63001 tgcgaactga attaaataaa ccagaatgac cagcaatacc agtaccgtat tcatcaccgt
    63061 cgcgttgtag ccgatgtagc catactgata gccaatctga cctaaaccac cggcaccgac
    63121 tgcaccaccc atcgcggaat aaccgaccag ggtaatcagg gtgatagttg ccgcattcac
    63181 cagacccggc aacgcttccg gtaacagcac tttacggacg atctgcattg gcgtggcacc
    63241 cattgcgcgg gaagcttcaa ttaacccggt tgggatctcc agcagagcgt tctcgaccat
    63301 acgggcaata aacggtgctg caccaacggt taacggaaca attgctgcct gcaaaccaat
    63361 cgatgtaccg acaataacgc gggtaaacgg aatcatccat accagcaaga taatgaacgg
    63421 gatggaacgg aaaatgttca caatcgcaga aacggtacga tacagcttcg cgttagcaat
    63481 aatttgcccc ggacgcgtga cataaagcag aacgccaacc ggcagaccaa tcacaaaccc
    63541 aaaaaatccg gatacgaagg tcattgccag cgtttcccat acgccacgaa ccagcagcca
    63601 catcatcggc tcagacataa cccagtacct ctacttttac atggtgttcc tgcagccagg
    63661 caatggcggc ttgcgtatct tgttgtgtgc cgtgcatttc agtcagcatg atgccgaatt
    63721 taacgccacc ggcgtagtcc atctgcgcgc taataatgtt gttgttgacg ttgaaacgac
    63781 gcgcggtttc agaaagcagt ggggcatcga ccgattgacc ggtaaactcc agacgcagca
    63841 tcggcacgca gtcagtaaat ggctccgttt gcagacgttc ctggtaatct tccgggatat
    63901 ccagatgcag ggtcgactga ataaacttct gcgccagcgg cgttttcgga tgcgagaaca
    63961 cttcacttac cgtgtcctgc tcgatcagtt cgccattgct gatgaccgcc acgcaatcac
    64021 aaatacgctt cacaacgtcc atttcgtggg tgatcaacag aatcgtcaaa cccagacggc
    64081 ggttaatgtc tttcagcagt tcgagaatag aacgtgtcgt tgccgggtcc agcgcgctgg
    64141 tggcttcatc acacagcaat actttgggat tgctggctaa tgcacgggca atcgccacac
    64201 gttgtttctg cccaccggaa agattcgacg ggtagctatc atgcttatcg ccaagaccaa
    64261 ccaatgacag caattccgtc acgcgacgtt tgatctcgtc tttcggtgtg ttgtccagct
    64321 ccagcggcag agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga
    64381 aaatcatacc aatctggcgg cgagctttgg tcaactcgga ttctgacagc gtggtcaatt
    64441 cctggccatc gaccagcacg ctaccctcgg ttgggcgctc cagcaggttt acacaacgta
    64501 taagcgtact cttacccgcg cctgaggcac cgataacgcc ataaatttgt ccagctggca
    64561 catgcaggct gacgttgttc aacgcctgga tggtgcgggt gccctggtgg aacactttgg
    64621 tgatattcga aagttttatc attgattatt tattatcgtc attaagttag tcgtagcatc
    64681 tcgaatgcct gaaacgggca acgccgtcaa tgaaatggat gttaaggcat ccagacgtct
    64741 aaatcaatca ggtttatgcg aagagcactt tcttgcaggt cgaaacatgc gatactagcg
    64801 tcccatgcct tattaaggag ctataaaagg tggcgaagag cgtacccgca atttttcttg
    64861 accgtgatgg caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat
    64921 ttatcgacgg tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gcgctggtgg
    64981 tagtaaccaa ccagtctggc attgctcgcg gtaaatttac cgaagcacag tttgaaacgc
    65041 tgaccgagtg gatggactgg tcgctggcgg accgagatgt cgatctggat ggtatctatt
    65101 attgcccgca tcatccgcag gggagtgttg aagagtttcg ccaggtctgc gattgccgca
    65161 aaccacatcc ggggatgctt ttgtcagcac gcgattattt gcatattgat atggccgctt
    65221 cttatatggt gggcgataaa ttagaagata tgcaggcagc ggttgcggcg aacgtgggaa
    65281 caaaagtgct ggtgcgtacg ggtaaaccta ttacacctga agcagaaaac gcggcagatt
    65341 gggtgttaaa tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcgc
    65401 aatgattaaa agatgaacgg ttgaaataaa aatgcatttt tccgcttgtc ttcctgagcc
    65461 gactccctat aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc
    65521 ggttcggttg aagagaaaaa atcctgaaat tcaggggtga ctctgaaaga ggaaagtgtg
    65581 atatacgcca cctcgcgaca gtgagctgaa agc
//
LOCUS       DAJTNM010000009        65419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTNM010000009 DAJTNM010000000
VERSION     DAJTNM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.009"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            132..575
                     /locus_tag="OO172_000669"
     CDS             132..575
                     /locus_tag="OO172_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5510962.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /gene="ybhA"
                     /locus_tag="OO172_000670"
                     /pseudo
     CDS             811..1630
                     /gene="ybhA"
                     /locus_tag="OO172_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OO172_000671"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OO172_000671"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5510963.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTCIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OO172_000672"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OO172_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5510964.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OO172_000673"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OO172_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5510965.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OO172_000674"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OO172_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5510966.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="OO172_000675"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="OO172_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5510967.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="OO172_000676"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="OO172_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5510968.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHREQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="OO172_000677"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="OO172_000677"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5510969.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="OO172_000678"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="OO172_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5510970.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="OO172_000679"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="OO172_000679"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5510971.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="OO172_000680"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="OO172_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5510972.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="OO172_000681"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="OO172_000681"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5510973.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OO172_000682"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OO172_000682"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5510974.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13936..14316
                     /locus_tag="OO172_000683"
     CDS             13936..14316
                     /locus_tag="OO172_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5510975.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="OO172_000684"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="OO172_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5510976.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OO172_000685"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OO172_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5510977.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OO172_000686"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OO172_000686"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5510978.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
     gene            complement(17417..17492)
                     /locus_tag="OO172_000687"
     tRNA            complement(17417..17492)
                     /locus_tag="OO172_000687"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="OO172_000688"
     tRNA            complement(17496..17571)
                     /locus_tag="OO172_000688"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="OO172_000689"
     tRNA            complement(17623..17698)
                     /locus_tag="OO172_000689"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="OO172_000690"
     tRNA            complement(17701..17776)
                     /locus_tag="OO172_000690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17913..17988)
                     /locus_tag="OO172_000691"
     tRNA            complement(17913..17988)
                     /locus_tag="OO172_000691"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17953..17955),aa:Lys,seq:ttt)
     gene            complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="OO172_000692"
     CDS             complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="OO172_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5510979.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18954..19475)
                     /gene="pal"
                     /locus_tag="OO172_000693"
     CDS             complement(18954..19475)
                     /gene="pal"
                     /locus_tag="OO172_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5510980.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="OO172_000694"
     CDS             complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="OO172_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5510981.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20935..22191)
                     /gene="tolA"
                     /locus_tag="OO172_000695"
     CDS             complement(20935..22191)
                     /gene="tolA"
                     /locus_tag="OO172_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5510982.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAK
                     AAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADI
                     NNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAA
                     KLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22256..22684)
                     /gene="tolR"
                     /locus_tag="OO172_000696"
     CDS             complement(22256..22684)
                     /gene="tolR"
                     /locus_tag="OO172_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5510983.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22688..23380)
                     /gene="tolQ"
                     /locus_tag="OO172_000697"
     CDS             complement(22688..23380)
                     /gene="tolQ"
                     /locus_tag="OO172_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5510984.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23377..23781)
                     /gene="ybgC"
                     /locus_tag="OO172_000698"
     CDS             complement(23377..23781)
                     /gene="ybgC"
                     /locus_tag="OO172_000698"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5510985.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23931..24224)
                     /gene="ybgE"
                     /locus_tag="OO172_000699"
     CDS             complement(23931..24224)
                     /gene="ybgE"
                     /locus_tag="OO172_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5510986.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24224..24337)
                     /gene="cydX"
                     /locus_tag="OO172_000700"
     CDS             complement(24224..24337)
                     /gene="cydX"
                     /locus_tag="OO172_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5510987.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24352..25491)
                     /gene="cydB"
                     /locus_tag="OO172_000701"
     CDS             complement(24352..25491)
                     /gene="cydB"
                     /locus_tag="OO172_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5510988.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25507..27075)
                     /gene="cydA"
                     /locus_tag="OO172_000702"
     CDS             complement(25507..27075)
                     /gene="cydA"
                     /locus_tag="OO172_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5510989.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27924..30557)
                     /gene="mngB"
                     /locus_tag="OO172_000703"
     CDS             complement(27924..30557)
                     /gene="mngB"
                     /locus_tag="OO172_000703"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5510990.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKIWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30575..32491)
                     /gene="mngA"
                     /locus_tag="OO172_000704"
     CDS             complement(30575..32491)
                     /gene="mngA"
                     /locus_tag="OO172_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5510991.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWESVDGPEAIDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTVQQDT
                     QPVKSVKTELKQALLSGISFSVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMQ"
     gene            32661..33383
                     /gene="mngR"
                     /locus_tag="OO172_000705"
     CDS             32661..33383
                     /gene="mngR"
                     /locus_tag="OO172_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5510992.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33487..34356)
                     /gene="sucD"
                     /locus_tag="OO172_000706"
     CDS             complement(33487..34356)
                     /gene="sucD"
                     /locus_tag="OO172_000706"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5510993.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34356..35522)
                     /gene="sucC"
                     /locus_tag="OO172_000707"
     CDS             complement(34356..35522)
                     /gene="sucC"
                     /locus_tag="OO172_000707"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5510994.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35616..36833)
                     /gene="odhB"
                     /locus_tag="OO172_000708"
     CDS             complement(35616..36833)
                     /gene="odhB"
                     /locus_tag="OO172_000708"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5510995.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36848..39649)
                     /gene="sucA"
                     /locus_tag="OO172_000709"
     CDS             complement(36848..39649)
                     /gene="sucA"
                     /locus_tag="OO172_000709"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5510996.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39950..40666)
                     /gene="sdhB"
                     /locus_tag="OO172_000710"
     CDS             complement(39950..40666)
                     /gene="sdhB"
                     /locus_tag="OO172_000710"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5510997.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40682..42448)
                     /gene="sdhA"
                     /locus_tag="OO172_000711"
     CDS             complement(40682..42448)
                     /gene="sdhA"
                     /locus_tag="OO172_000711"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5510998.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42448..42795)
                     /gene="sdhD"
                     /locus_tag="OO172_000712"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42448..42795)
                     /gene="sdhD"
                     /locus_tag="OO172_000712"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5510999.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42789..43193)
                     /gene="sdhC"
                     /locus_tag="OO172_000713"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42789..43193)
                     /gene="sdhC"
                     /locus_tag="OO172_000713"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5511000.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43574..43681
                     /locus_tag="OO172_000714"
     CDS             43574..43681
                     /locus_tag="OO172_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511001.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43887..45170
                     /gene="gltA"
                     /locus_tag="OO172_000715"
     CDS             43887..45170
                     /gene="gltA"
                     /locus_tag="OO172_000715"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5511002.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45561..46127
                     /locus_tag="OO172_000716"
     CDS             45561..46127
                     /locus_tag="OO172_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5511003.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATCVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46178..48637
                     /locus_tag="OO172_000717"
     CDS             46178..48637
                     /locus_tag="OO172_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5511004.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALALEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGQVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48652..49383
                     /locus_tag="OO172_000718"
     CDS             48652..49383
                     /locus_tag="OO172_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5511005.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49380..50442
                     /locus_tag="OO172_000719"
                     /pseudo
     CDS             49380..50442
                     /locus_tag="OO172_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50594..51640
                     /locus_tag="OO172_000720"
     CDS             50594..51640
                     /locus_tag="OO172_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5511006.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51637..52428)
                     /gene="nei"
                     /locus_tag="OO172_000721"
     CDS             complement(51637..52428)
                     /gene="nei"
                     /locus_tag="OO172_000721"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5511007.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52464..53198)
                     /gene="pxpA"
                     /locus_tag="OO172_000722"
     CDS             complement(52464..53198)
                     /gene="pxpA"
                     /locus_tag="OO172_000722"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5511008.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53188..54120)
                     /gene="pxpC"
                     /locus_tag="OO172_000723"
     CDS             complement(53188..54120)
                     /gene="pxpC"
                     /locus_tag="OO172_000723"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR5511009.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(54114..54770)
                     /gene="pxpB"
                     /locus_tag="OO172_000724"
     CDS             complement(54114..54770)
                     /gene="pxpB"
                     /locus_tag="OO172_000724"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5511010.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQQHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54793..55536)
                     /gene="ybgI"
                     /locus_tag="OO172_000725"
     CDS             complement(54793..55536)
                     /gene="ybgI"
                     /locus_tag="OO172_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5511011.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55807..57288
                     /gene="dtpD"
                     /locus_tag="OO172_000726"
     CDS             55807..57288
                     /gene="dtpD"
                     /locus_tag="OO172_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5511012.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57438..58856)
                     /gene="phrB"
                     /locus_tag="OO172_000727"
     CDS             complement(57438..58856)
                     /gene="phrB"
                     /locus_tag="OO172_000727"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5511013.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYETARKGK"
     gene            complement(58853..59362)
                     /locus_tag="OO172_000728"
     CDS             complement(58853..59362)
                     /locus_tag="OO172_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5511014.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59491..59808
                     /locus_tag="OO172_000729"
     CDS             59491..59808
                     /locus_tag="OO172_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5511015.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(59863..60243)
                     /locus_tag="OO172_000730"
     CDS             complement(59863..60243)
                     /locus_tag="OO172_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511016.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     EEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYTL"
     gene            complement(60246..64910)
                     /locus_tag="OO172_000731"
     CDS             complement(60246..64910)
                     /locus_tag="OO172_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5511017.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGGTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYWFRHDVLDRLAEQINPDGCRQSYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLLEIRRQRHETDAPEVIRFSYD
                     RLGRMLSEETAQGILTHQYDELGNRTATTFPDGRTQRHLYYGSGHLQQINLDREVISE
                     FTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGMRGVVPETFTDRQYSYSGEDE
                     LLKTRHSRRGETDYFYDPTGSITACRSEDEGYLASWQYDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TGNKTKAGSYHWDDIKDPSVTKPPYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPR
                     ET"
     gene            complement(64929..65132)
                     /locus_tag="OO172_000732"
                     /pseudo
     CDS             complement(64929..65132)
                     /locus_tag="OO172_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15834 a        17736 c        15985 g        15864 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 accacttgta atcaattgat tataaaggat taattcataa agagcattta cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgcag gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gatgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag atgacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catcgtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgccctct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggcgga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tataccaacc gcatcgccaa tagtcgttat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattacagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagtatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcgccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatga gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgtcgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctaga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctttcctaca atttctaact gtaattcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca cgccaccatt cagatggaat
    15301 atcaaccttg tcatgggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 accgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggcca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgtttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgcaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcgc attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttacattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtggtgggtc
    17821 gtgcaggatg actcgacttc gtctcgccct tcggcgccgt tgctgatgca acgttatcct
    17881 tcacatttta ctaccgcttc caccttagcg attggtgggt cgtgcaggat tcgaacctgc
    17941 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccac tttttcgttg
    18001 ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt tcagacttga
    18061 gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg acacgaccag
    18121 aaataatgcg acttctggtc gtgttttatg cattacatcg cgttcagacg tttttgtgcc
    18181 tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac ggctttcgct
    18241 tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc atcagcagcc
    18301 tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc atcatctttt
    18361 ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag gtaagttgaa
    18421 tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg gcgggattta
    18481 tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcgccgct tttcaccggc
    18541 gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc cgcaccgctt
    18601 tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac
    18661 aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc ctgaatctga
    18721 ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag ttgggttaaa
    18781 agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc gaccgagcct
    18841 gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc tataccaacc
    18901 agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa
    18961 accagtaccg cacgacggtt tttggagtat gctgcttcgt catgacccag tactgcaggt
    19021 ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt accctgcagg
    19081 tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc cggagtacca
    19141 cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg caggaagttt
    19201 gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc cagatcgaag
    19261 taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc ttcggaagac
    19321 atgttgccgt tgccgccgtt cgcatccata ccagtgccag cacccagcat gccttcgctg
    19381 ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac aggcagagca
    19441 atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt actattcaat
    19501 taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat cagttgccgg
    19561 aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc ccatcccctg
    19621 agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt catccaggaa
    19681 cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg
    19741 cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg
    19801 gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat tcactttata
    19861 aacctgcgga cgaccggcct ggtcagaagt aaatgccagg ttctggctat ccgggaacca
    19921 ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac cagaagccaa
    19981 atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca gtttgctgcc
    20041 gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct gacgtacagc
    20101 gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg tcacataagc
    20161 cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg aacggtgaac
    20221 gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga actgaccgcc
    20281 gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc cggtcagttt
    20341 ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct tgttcacttt
    20401 gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc
    20461 aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag cgtcaatgcc
    20521 cagtgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct gtggcagacg
    20581 agcgcgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa cgatgccgcc
    20641 aatatcttca ggtgccgcac caggccccgc ccactggaaa ggaacaacac caataggacg
    20701 accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa ctgatgccca
    20761 cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc ccttatctgg
    20821 accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca aaactaccag
    20881 aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac agtcaacatc gcgattacgg
    20941 tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg gtggtttcgg
    21001 gatcttcgca agtttagctg ctgccaacgc agcctgacaa agtgcgggat cgccaccttc
    21061 aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg tacaggtttt
    21121 gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa tctgcccggc
    21181 atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac tcccggcagg
    21241 cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac cagagcttag
    21301 ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt tttcagctgc
    21361 tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg ctttcttatc
    21421 agctgctgcc ttatcagcag ccgctttttc tgctgctttt ttgtcggctg cagctttctc
    21481 tgctgccgct ttcttatcag ctgcagcctt atcagctgca gccttttcag cagccgcttt
    21541 cttctcagct tctgccttgg ctttttcagc agcttcagtt gccgctttct ttcttgcttc
    21601 agctgcagct gcttctgccg cttccgcttt cttcttcagt gccgcagcgg ctacctcggc
    21661 ttttttctgc gcttcggctg cggctttggc ggcttctgct tctgcttttt tcttcgcgtc
    21721 tgcagccgct ttcttcgcag cttcttccgc agctttagca tctgccgccg ctttcgctgc
    21781 cgccacttca gcttgcttct gctttaactc ggcctgtttt gcggcttctt cagcctgctt
    21841 tttctgctcc tgagccgcta accgctcttt ctcaagttgc ttcaggcgtt cctgttcagc
    21901 cgcttgtttc tcacgcagtt cttcagcagc ctgctgttcc ttcatcttgc gctgttcatc
    21961 agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa ctaccgcacc
    22021 tgaatcaacc atgacagcgt cgatggacga accaccgccg cctccggctg aagcttctat
    22081 attctcatcg aacgaactcc agatcagcgc cgcaaataag atgacatgca gcactgctga
    22141 aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca ctctcggttt
    22201 ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca gatgtttaga
    22261 taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag ttcagtgctt
    22321 taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc gttttcgggt
    22381 tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa cgctccaggc
    22441 gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca atcactggcg
    22501 gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg acctccacgc
    22561 tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc agtacgtcca
    22621 gcaacggtac aatgttgatt tcggacttga gatcgcgacg acctcgtcca cgcgctctgg
    22681 ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc agaatcgcgg
    22741 taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc tggttgagac
    22801 ggttataagc cattaccgcc ggaattgcgg cgaacagacc aattgccgtc gcaatcaacg
    22861 cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc ccgagggcga
    22921 taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg ctgatggagc
    22981 caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacgg ttcatggaga
    23041 tacgcatagc acgcgacgcc ccttccacta cggcttccgg cgcatggcta ttggcacgat
    23101 gcaggcgcac aaactctttg aacccgctgt aaaagatttg ttccgaacca gtcagattat
    23161 cccgtttccc ctgactctct tgatagaggc gagagagttc gattccagac cagaatttat
    23221 cttcaaacgc ttcggcttca cgcgccgctg cgttaagaat acgggttcgc tggataataa
    23281 tggcccagga tgcgattgaa aaaccaatca aaatcaacat gataagttta accagaaggc
    23341 tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact ccgcgacaat
    23401 agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa ccagaacctc
    23461 tgcttcattc agcaaagtat tctcggcgtt gacaatatgt tgcgtgaaaa ccaaagaggt
    23521 gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc gcgcaggtgc
    23581 ataatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca tcagcgcctg
    23641 ctgactgaag tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa aagcgacgta
    23701 actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc gaaccggcca
    23761 tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta gagcttttaa
    23821 acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaagtta agggagcgta
    23881 aaaatttatg ggcctagagc ggcccataat tttttaaaga gaattctgat ttaaaagaag
    23941 aagaaaatca gcccgacaat gagaacaata tcggcaagca atgggcaaaa aatcccttgc
    24001 caaagaacct tctgcggacg aaagcccacg ccgtgaatca caccagcaca gacggcccac
    24061 atcagcaata aaccatgcca gatttccagt tcactggtct tcgcggcaaa acgagatggg
    24121 tcccaaaaca tacatcctgc taacagaagc gccatcacga aggaaagcgc ccgcaggggg
    24181 cgcttgtcca ttaccgcata caaggtcgcg ataatcttac tcatcagatg tcttcttgac
    24241 cggctttgcc tgattcgacg tgttcaagcg ccagcgcggt gattacccca aacgaacagg
    24301 caagaagcgt tcccagaatc catgcgaaat accacatttt tagctcctta cttagtacag
    24361 agagtgggtg ttacgttcaa tatcttcttt ggtgatacga ccgaacattt tccagtaaca
    24421 ccaggcggtg tagagcagaa tgatcggtac cagaaccacc gcaacccagg tcatgacgtt
    24481 aagcgtcagc tggctggaag ttgcatccca cattgtcaga cttgcgttca tcatggtgct
    24541 ggacggcatc acaaacggga acattgcgat accggctgtc aggatgatgc aggccagcgt
    24601 cagggaggag aacacaaacg cccacgcggc tttatccata cgtgcagtca ggatggtcag
    24661 cagcggcaga accacaccca gtgccggaat agcccacaga attggcgtgt tgttgaagtt
    24721 aaccagccat gcgccagctt cacgaaccac ttctttattc agtgggttag aggctgcgta
    24781 atggtccatt gtcgatttca cgacataacc atcgataccg tacatcaccc atacgccagc
    24841 cagtgcgaaa cagaccagtg tcaccagcgc agccacctga gccgttgcac gggtacgcag
    24901 gtgcagttcg cccccggtac gcatttgcag ataggttgcg ccctgagtaa tgatcatccc
    24961 tacgctcacc acgcctgcca gcaggccgaa cgggttaagc aactggaaga agttaccggt
    25021 gtagtacaga cgcagatatt catcaacgtt gaacggtacg ccctgcaaca ggttaccgaa
    25081 cgctacacca attaccagcg gcggaacgaa gctaccaatg aagatgcccc agtcccacat
    25141 gttacgccag cgggtttctt caatcttgga gcggtagtca aaaccgaccg gacggaagaa
    25201 caaagacgcc agcacgagga tcatcgccac atagaagccg gagaacgcag cggcatagac
    25261 catcggccag gcagcaaaga gtgcgccgcc cgcggtgatc agccaaacct ggttaccgtc
    25321 ccagtgtggt gcaatggagt taatcataat tcgacgctcg gtgtcgttac gaccgaggaa
    25381 acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg actgcaaaac caatcagcag
    25441 aacgccaacc agcagccacc agataaaacg caatacttca taatcgatca tttgacgact
    25501 cctgtcttag cgtgccggct gagtagtcgt ggaagactgc tcaaagtgat agcgaccggt
    25561 tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt aagaacaatt ctgccaccag
    25621 gaacagggta tacaggccgc aaatcagcac cattgagaag atgagatcgc ctgcggtcag
    25681 tgacgaattc gccacagctg tcggcagcac ttcaccgata gcccacggtt ggcggccata
    25741 ttcagccacg aaccagcccg cttctacagc aatccacggc agcggaatac cgtacagcgc
    25801 ggcgcgcaga agccattttt tctcgccaat gcggttgcgg atgacactcc agaaagagag
    25861 cgcgatgatt gccagcagca ggaagccaca cgccaccatg atacggaacg caaagtacag
    25921 cggcgctaca cgcgggatgg agtctttggt tgcctgttga atctgcgctt cagtcgcatc
    25981 agccacgttt ggcgtatagc gtttcagcag cagaccgtaa ccgaggtctt tcttcatgct
    26041 attgaactgg tcacgaaccg cctggtcggt agaaccagaa cgcagttgtt cgagcagaga
    26101 gtacgccttc atcccgttac gaatgcgttc ttcatgctgc accatcagct ctttcaggcc
    26161 gataaccggg gtatccacgg aacgcgttgc aatgatgccc agtgcgtaag ggatctgaat
    26221 cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg aacaaagtaa aggcagcagg
    26281 cgcaggttgc gtttcccact cggcttcaat agcagccagt ttggttttct gcacgtcgcc
    26341 catttcgtag ccggattcat cacccagaac aataacagac agaacagcag ccataccgaa
    26401 gctggcagcg atagcaaagg agcgtttagc gaaggcgaag tcacgacctt tcagcatata
    26461 ccatgcgctg ataccgagga tgaacatcgc gccagtcaca taaccagacg ctacagtgtg
    26521 aacgaatttc acctgagcaa ccgggttaag caccagctcg gagaagctca ccatctccat
    26581 acgcatagtt tcaaagttga aatcggacgc gattgggttt tgcatccagc cgttcgcaat
    26641 cagaatccac agtgcggaca ggtttgaacc gagcgccacc agccaggtga cacacatatg
    26701 ctgaacttta cccagacgat cccaaccgaa gaagaacaga cctacaaagg tggattcgag
    26761 gaagaaggcc atcagacctt cgattgccag cggcgcaccg aagatatccc ctacatagtg
    26821 ggaatagtaa gaccagttag tcccgaactg gaactccatg gtcagaccgg tagccacacc
    26881 cagagcgaag ttgataccaa acaacttgcc ccagaacttg gtcatatctt tataaatctg
    26941 tttgccggag aggacgtaga ccgtttccat aatggccagg aggaacgcca taccgagcgt
    27001 cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca aactgtaagc gcgacagttc
    27061 gactatatct aacatcatga ctccttgctc atcgcatgaa gactccgaga gtgaaccccg
    27121 ttagaaaggg tcacacgcat gccccaatag aaatttgttt gaacactttc atcataccat
    27181 gtgaatgcac acaaaaacgg cgtaaagaaa ggttgcaaac atgttaataa aaactcaaat
    27241 tgatcccacg tatatattac gccgcaaaat ccttacaata aacaggtttt tgttgaagca
    27301 aatttgcatt tttcgacggt gatcaattta tagctaaatt gccgcctttc agccaatttg
    27361 atcgagaaca atttatctct ttttgatgcc catttccaag acttatacat tgataaatat
    27421 caatttctac aaattatgtt aatcatgttt caacaaggta actttagtga catttatgtt
    27481 taaaatgtgt gaattataag actgaaatta gtttcggttg gcaattaaag ataaaaagag
    27541 acgaagcaaa aacagcacgg ttgaccttaa ttcttttttt ggtcactggc taatacgacg
    27601 cagagtttca ggattattaa taaaggcata aatctcctcc ccgccgatat taacatttaa
    27661 ttaacttata attatattat aattaactca cccggggtta ttattttgtt aaaaataaag
    27721 ccacatatac gtagcttgag agtgaagata aacgcaaaat tacctgatgc gctacgcttg
    27781 ccagtgtcta catcatctct gcaatatatt gaatttgcct gcttttgtag accgaataaa
    27841 gcgttcatgc cgtatccggc atgaacaaag cgccctttgt caacaatctg gccgcgcata
    27901 tgtggcttcg gtttgattgc tattcaggca agccggtaac tgaacgtccg tgactggccg
    27961 ggtaaaaaag gccccgatag atttgaacct tgattttcct cggtggtaat gtgttcatcc
    28021 atcatcgttt ctgagcaaga aatcacctcg cgactgaaag caacagtcgc atcacaggtt
    28081 gctgattcag ccggattaaa cagccgtaaa atcacttctt gtcggtcttc agctttctta
    28141 agtgcgctta tcaggcatcc cactgggggc attttcaaca aactataact ttccggcacg
    28201 ttgaatccgg ctttgttgag cttcatcgca tcccatggga ttttgttgta acactgtact
    28261 ggagtcagcc atgctcgcgc ctgctgagct acaccagcgg cggttggcgt accggtataa
    28321 ctcaataaac ttaggcgaca agaaagcaga ccacgtagtt gtgagtccgg gactggcatt
    28381 ttaattcccg aaggccgcct aggccttaaa agcagatctt ctttccccag taagcccacg
    28441 ccgcgcagca acgtaatggc aaaggttttc ttctcttcac cgatgacttc aaattcacgt
    28501 agcccttcgc taaagacagc catgccgtta cgcccttcct gtaaggctgc atagttgagc
    28561 atattccata ccggaaccgg cgcttctttc cagccttctt gctgccagtt gttcattgca
    28621 ctgtcgttca cggggcgcgt tagcgaacca aactgggtat ctgccagaac actatcggtg
    28681 ttaaaagatg tagggatcag gacgcgaagg cgatgatcgt cagcctggtt atcaaggttg
    28741 atatccacat caatgcgcct gttgttatga ctaagagtga caaccaactc gacacctact
    28801 ctgccagtgg attgtcttgc gctgcgttgt tgcagattac gcggcaccgc catttcatag
    28861 cggataacag ccctgctctg ccaggcctca tgaataatct cgcattgcgg tttcgcattc
    28921 gccgaagtaa ttacccactc ttcttttgcg ggtgaatagt catattcatc accatcatca
    28981 gagctttctt caatttgcaa cacccggtca tagcgcacac cgctatcttt atctaccagt
    29041 tgtagcgttc catcctcatt gagcgcaatt tgccagaaag cattttccag tatcccttca
    29101 gcgtcacttt ttgccgcaat cacgttgcca ggctgattcg cttcgatata aagcgtgcga
    29161 tagcccatag aagggacaat ctggttgatc tgtatatcaa actccataaa gggatcgtaa
    29221 ttaccgtaat gaactatttg ccgatcgatt aggcctggat cgatctcacg ggcatggcga
    29281 ataaaatacg gtacaggctg accgcgatcg tctcgtagat taaactggct ggcgcgcagc
    29341 cgcacagtgg tgttgatcac ttcttcccgc ggccagggca tcaggttaaa caggacgagt
    29401 ttgtcggcgt cgctctgcgg catgttgtcg gcgattttac gcatatagaa acgcagcaga
    29461 ttatccgcca tgtcttcagc cagttcgaag cgggcgacga tttcgcgatg aactttgtca
    29521 ctacagcagc agccgatgct gtcgtgggca tgatttttta agatctcttt ccatattttc
    29581 tccagcaagc cgtggtggta ttcaaaaccc aacgtccagg ccagcgttgc cagcggttcc
    29641 agcagattaa caatcttatt ttcaatacgt gcgtgggcaa ctttgatatc catgcgcgtg
    29701 gaaccgatgg tgcgatgcac gcgcatatat ttgccatcaa taaattcccc tttcagggtt
    29761 gccagattat ctcgctgcgc ttcgatcttc tcaaatacct cttcaaagcg gctcatcaca
    29821 aactttcgtt gaggataaat ttcgcgcagt ttctccatca cttcgaagat attttgctgc
    29881 aatggcatct gatcatgccc attcggcagc aaaatctctt tggttacaga cgctttttcc
    29941 agcacgtcaa aataactgtc gaggcgtttg cggaatccgt tttcgtccgc aggtaagtac
    30001 ttaccgatgg cataacccaa cggcatcacc tgcgccgtca cttcgctacc gtcactgctt
    30061 tgccacaaaa actcggtttt atccgtaccg tggcgctcag aacatccacg ccagaacatg
    30121 gtgcaggtaa tgccaaatcc attgtagata tgcggcagtt gcccggacat gccaaaggaa
    30181 tcgggtaaat aacctatttt catcggctcg ccaaacgcga ggcagtcacg cattccgtac
    30241 atcagattac ggacgatgga ttccgcagaa acaatcgtgg tatcggtctg ggtataccag
    30301 gggccgataa tcaacttgcc ggcttctacc agtttcttca cgcggtcttt gttttccggt
    30361 ttcaccgcga aataatcttc gaggatcgcc gtttgcccgt cgagtacgta atatttgtat
    30421 tcgttgtcct gttccagtcg gcacaggatc tcttccatat tattgaccag cagaatgcgt
    30481 gactcttcgg tggtgaaata ccactcccga tcccagtgca tatgcggggt gatgtgaacg
    30541 cgagataccg ctttcatcgt cgtttccttt ttgtttattg cattacgcca tcagtcagat
    30601 agttgccatg cttaaccgcg tgacgccgcc agatcaggag aattgcagtc gagattgcag
    30661 cccccaccaa tgccgcacca aaccagccaa ttgccgccat aacaccgccc gcgccattat
    30721 catgaagtaa aaagagcgag aaaatgccag caccgggtgt cgaaaggccg atattcatcg
    30781 caccgacaat agcgcccgtt accatagagc ccagcacaaa cgaaccaata acccgcagcg
    30841 gatcttcaat cgccatcggg atcgcccctt cggtaatacc tgccagccct aacagccagg
    30901 tggatttccc ggtctcaatt tcaaactctt taaacaggcg cggtgcgagc atcgtggaag
    30961 cggttacggt aaatgccgaa accattttga cggaggcgaa aatggcatac gggccgtaaa
    31021 cgccgttcgc cattgcgccc aggcagaatg cataagcggc tttattcact ggccctccaa
    31081 ggtcaaagga acacataaaa ccgagaatgg cacccagcaa cagcgcgtta cttcctgaca
    31141 gaccgttcag ccaggcggta agcgagttat tgatccaggc gacaggttcc cccaccacaa
    31201 acagcatcag actgcccgct cccaatgtac cgagcaccag gtagagataa aaagtcagga
    31261 agccattgaa tttactgcta agacgcaagt gatttttcat ccagcgtatc aagtaaccgg
    31321 ctatcaatcc gccaacgacc gcgccgagaa acccggagcc gatcatgttg gcggcaagtc
    31381 cagccgcaaa gcctggcgct aacgccggtt tatctgccag cgaataggcg gtataggctg
    31441 ccagcaccgg taccatcaaa attccgagca gcccgccgcc cagtttgcgg tacatccaca
    31501 gccaggagtt ttcttcatta aacagatctt gtagcccgaa gatttgcgac agtaataccg
    31561 cgaccgccag caccgtcccc cccgcgacaa tcaacggtac ggaaaaagag atcccgctca
    31621 acagtgcctg tttcagctcc gttttgacgc ttttcaccgg ttgcgtatct tgctgtacgg
    31681 tacgcgtctc atcgccacgc tcgagggtaa gcgattgttg gatcaacgct tctgcatggc
    31741 gaatcggctc ggcaacaggc acagaaagcg caggaatgcc gttaaaacgc tcactctcct
    31801 tgatggcgac ttcagccgca aaaatacagg cagttgcact attgagttga tccgccgtta
    31861 aacgcccttc aataccgtta gcaccttgct tttcaacgta gacattcacg ccgagtttgc
    31921 gtccggcttt ttccagatac tccgcagcca tgtaggtgtg agcaataccc gccgggcagg
    31981 ccgttacgca aacgatagtt ggcgcgttgg aaaaagaggc agaaggttgc gtgcctccct
    32041 tgtcatccag cgccgagagc aattcatcag gcgtcgtcgc cgactgtata cgcgcccgaa
    32101 tctcatcatc cgccaggcgc gtggtcagcg ctgtcagcag ttgcatgtgc gtagtacccg
    32161 cttcattggg gggaatcgcc agcaacacca ctaaatcaat cgcttccggg ccatccacgc
    32221 tttcccactg aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct tttaccgcag
    32281 cagttttgcc atgcggcaca gccaaccctt cacctaaggc ggtcgggcca aggctttcac
    32341 ggcgatacac ttctttcaga aattgctcag tactggaaat tttccccaga gcagcaagac
    32401 gttgagtcaa cgcgtgaatg gcctcttcac ggctggtaaa gcgcgcattc agacacaacg
    32461 catcgcggtg ggtcagagtc gtcaggttca tcatgatcct cacttgatcg tttttatagt
    32521 cgcgccagtg tgcccgataa aacaatacca ttgcgtaatc aatttcactt gattgaaatt
    32581 atttgcattg tatttgtatt atttacagga tgaatttatc gggcataatg gctaaattcg
    32641 tattaatgag atgctaaata atgggacaca aacccttata ccgacagatt gctgatcgca
    32701 ttcgtgagca aattgcgcgt ggcgagctta aacctggcga tgcgttacct accgaatcgg
    32761 cattacaaac agagtttggc gtcagccggg ttacggtgcg tcaggctttg cgccagttag
    32821 tcgagcaaca gatcctcgaa agcattcagg gcagcgggac ttacgtcaaa gaagagcggg
    32881 tcaattacga tatttttcag ttaaccagtt ttgatgaaaa actgtcggac cgtcacgtcg
    32941 atacacacag tgaagttctg atatttgaag tgattccggc tgacgatttt cttcagcaac
    33001 agctacaaat caccgtgcag gatcgtgtgt ggcatgtgaa gcgcgtgcgc tatcgcaagc
    33061 aaaagccaat ggcgctggaa gaaacctgga tgccgcttgc gttgttcccg gatctcacct
    33121 ggcaggtcat ggaaaattcg aaatatcact ttattgaaga agtgaagaag atggttatcg
    33181 atcgtagcga acaggaaatc attcctctga tgccaaccga agagatgagc cgtctgctta
    33241 atatcagcca gacaaagccg attcttgaaa aagtatcgcg tggatattta gttgatggtc
    33301 gggtgtttga atatagccgc aacgctttta ataccgatga ctataaattt accctgatag
    33361 cgcagcgaaa atcatcgcga taagcacaaa aaaggccatc atacgatggc caaccatgtc
    33421 gaaaccgaca gcccttcgcc attcctgtag agggaagcgg cgagggctat ttcttattac
    33481 agatatttat ttcagaacag ttttcagtgc ttcaccgata tccgccaggc tgcgaacggt
    33541 tttcacgcct gcggcttcca gagcagcgaa tttctcatcc gcagtccctt tcccaccggc
    33601 aatgatcgca cccgcgtggc ccatacgttt gcctttcggc gcagtcacac cagcgatgta
    33661 acccacaact ggcttggtaa catgctcttt gatgtacgct gccgcttctt cttcagcgct
    33721 accgccgatc tcaccgatca tcacgatcgc ttcggtctgc ggatcttttt cgaacatttc
    33781 gagaatgtcg ataaagttag agccagggat cgggtcaccg ccgataccga cacaggtcga
    33841 ctgaccgaaa ccgtaatccg tggtctgttt aaccgcttca taggtcagtg taccggaacg
    33901 ggaaacgata cccactttac ccggtttgtg aatgtgaccc ggctggatac cgattttgca
    33961 ttcacccggg gtgataacgc ctgggcagtt cgggccgatc atacgaacgc ctgcttcgtc
    34021 cagtttcact ttcacggtca gcatatccag cgtcgggatg ccttcagtga tggtgataat
    34081 cagtttgatg cctgcatcga tagcttccag aatggagtct ttgcagaacg gtgctggtac
    34141 gtaaataaca gacgcggaag cgccagtagc agcaacagct tcacgcacgg tgttgaacac
    34201 cggcaggccg aggtgggtag tgccgccttt acctggagtt acgccaccaa ccattttagt
    34261 gccgtatgca atggcctgtt ctgagtggaa agtcccctgg ctaccggtaa agccctggca
    34321 gataaccttg gtgtttttat caattaaaat ggacattatt tcccctccac tgcggcaaca
    34381 acctgctgag ctgcatccgt cagacctttt gctgcaataa tattcaggcc gctgtcagcc
    34441 agtttcttcg cgccgagttc ggcgttgtta ccttccagac gtaccacgac cggtacgtta
    34501 acacccactt ccgctaccgc gccgatgata ccgtcagcga tcaggtcgca acgaacgata
    34561 ccgccgaaga tgttaaccag aacggctttc actttgtcgt cagagaggat gattttgaac
    34621 gcttcggtta cacgttcttt ggttgcgccg ccgccaacgt ccaggaagtt agccggttcg
    34681 ccgccgtgca gtttaactat gtccatcgta cccatcgcca gacctgcgcc gttaaccata
    34741 caaccgatgt taccgtccag cgcaacgtag ttcagttccc actgtgcagc ctgtgcttca
    34801 cgcggatctt cctgcgactg gtcacgcatt tcgcgcagat caggctggcg gaacagggcg
    34861 ttgccatcag cgcccagttt gccgtcgagg caaatcagat cgccctgttt ggtgatgacc
    34921 agcgggttga tttcgatcag cgccaggtcg cgctccagga aaatggtcgc caggcccatg
    34981 aagattttgg tgaactgctg aaccagttta ccttccagac ccagtttgaa cgccagctcg
    35041 cgtccctgat acggcatcgg gccagtcagc ggatcaagcg caactttatg gatcaggtgc
    35101 ggagtttctt ccgccacttt ttcgatttcc acgccgcctt cggtggaggc cataaagacc
    35161 acgcgacggg aactacggtc tacaacggca ccgagataca gctctttagc gatatcggtc
    35221 gccgcttcaa ccagaatctg gttaaccggt tggccattgg catctgtttg atacgttacc
    35281 agacgcttgc ccagccagtt ttctgcaaaa gcacggatgt cttctttgct gtttacaact
    35341 ttcacaccgc ccgctttacc gcggccacca gcgtgaacct gacatttcac tacccacgga
    35401 ccggcaccga tttttgaagc ggcttcttct gcttcgcgcg gagtagtaca ggcataaccc
    35461 accggtgctg gtaagccata gcgggcaaac agttgttttg cctgatattc atgtaagttc
    35521 atgtgttctg tccatccttc agtaatcgtt atcttttaaa ccgtagaccg acaccctatc
    35581 cacgtcggtc tacagtgcag gtgaagctta aactactaca cgtccagcag cagacgcgtc
    35641 ggctcttcca gcaactcttt gatcgttacc aggaagccca cggattcgcg accatcgatc
    35701 agacggtgat cgtaggacag cgccaggtac atcatcggca ggatctcaac ctgaccattc
    35761 accgccatcg gacgatcttt gatagcgtgc atacccagaa ttgcgctctg cggcgggttg
    35821 atgatcggcg tagacatcag ggaaccgaac acaccaccgt tggtgatggt gaagttacca
    35881 ccggtcagat cttcaaccgt cagcttgcca tcacggcctt tgactgccag ctctttgatt
    35941 ttcttctcga tgtctgccat gccgagggta tcgacatcac gcagaaccgg cgtcaccagg
    36001 ccgcgcggcg tagaaaccgc catgctgacg tcgaaatagt tgtggtaaac cacgtcatcg
    36061 ccgtcgatag acgcgttcac ttccgggtaa cgtttcaggg cttcaaccac cgctttcacg
    36121 tagaaggaca taaagcccag acggatgccg tggcgttttt caaacgcttc accgtactgc
    36181 ttacgcagat ccataatcgg cttcatgttg acttcgttga acgtggtcag catggcggtg
    36241 gagtttttcg cttccagcag acgctctgcc acacgcttac gcaggcgagt catcgggaca
    36301 cgtttttcac tacgtgcagc cagagccggt tgcgccgccg gagcagccgc tgccggagca
    36361 gactctttcg ccggggcttt cgccagatgt ttttccacat cttcacgagt cagacgacca
    36421 cccacaccgg tgcctttaat ggcgctggcg tcgagattgt gttcagccag caggcgacgg
    36481 atcgccgggc ttaacgcatc gttgttttgc tcttccagag acgcctgctg gcgttgcgcc
    36541 ggagtggacg ctttctcttc agatttggcg ctggtttctt taccggcgct gttgccttca
    36601 cgcaggcgac caaggatctg acgagacgtt accgttgtac cttcatcttc cagaaccgca
    36661 tccagaatgc cgtctgctga tgccggtact tccagtacca ctttgtcagt ttcgatttct
    36721 accagcactt catcacgtac gactgcgtcg ccgggttttt tatgccaggt tgcgacggtg
    36781 gcatcggcta cggattcagg caggtcaggg accagaatat ctacgctact cattgtgtat
    36841 cctttattta ttcgacgttc agcgcgtcat taaccagatc ttgttgctgt ttctggtgaa
    36901 cggacatata ccctaccgcc ggagaggcgg aggccgggcg gcctgcataa cgcagagaag
    36961 ccccaaacgg aatcacttca cggaaatgat gctggctgca gtaccatgcg ccctggttga
    37021 gcggctcttc ctggcaccag acaaaatcct tgacgtgagc aaactgctgc aacacttcct
    37081 gcatcgcttt atgcgggaac gggtagagtt gctcgatacg cacaatggcg acatcgtgtt
    37141 gattgttctt acgacgctgt tccagcaggt cgtaataaac cttaccagaa cacatcacta
    37201 cgcgcttcac gcccttcgga tcaagttcgt cgatttcacc gatggctggc aggaaggtgc
    37261 cgttcgccag ttcttcgagg ctggaaaccg ccagcggatg acgcagcagg gatttcggcg
    37321 acatcacgac cagcggacga cgcatcccgc gcagcgcctg acgacgcagc atgtggtaaa
    37381 cctgtgccgg ggtagacggt acgcaaacct gcatgttttg ctcagcacaa agttgcagat
    37441 aacgttccag acgcgcggag gagtgctccg gcccctgccc ttcgtaaccg tgcggcagca
    37501 acatcaccag accacacatc cggccccatt tctgctcgcc agaggagatg aactggtcga
    37561 taaccacctg cgcaccgttg gcgaagtcac cgaactgcgc ttcccagatg gtcagggtgc
    37621 gtggttctgc ggtggcataa ccatattcaa acgccagcac ggcttcttct gacagtacgg
    37681 agtcccagac acggaacgcg ccctgaccgt tatggatgtg ttgcagcggc gtgtaagtgg
    37741 aaccgttaga ctggttgtgg atcaccgcgt ggcggtggaa gaaggtaccg cgaccggagt
    37801 cttcacccga caggcgaacc ggaatgcctt catcaaccag cgtggcgtaa gcgaggtttt
    37861 ccgcaccgcc ccagtcgaac agtttctcac cggctgccat cgcctggcga tcgccataaa
    37921 tcttggcaac gcgagactgc atttcaactg cttctggtac cgtactgata cgtttagcca
    37981 gttcctgcag gcgcttcatc tcaactttgt tcgggtactc ttcgtcccat tcgtggttga
    38041 ggtacggcga ccaggtgaaa gagtgcatgt tcatcggacg ccactctgcc actacgcaat
    38101 cgccagcatc cagcgcatcg cggtacaggt taaccatctc ggtggcatct tccagcgtcg
    38161 ccactttttc ctgctccagc ttgtcagcgt agattttgcg cggtgtcgga tgttttttga
    38221 ttttctgata catcagtggc tgggttgcgc tcggctcgtc ggcttcgttg tggccgtgac
    38281 ggcggtagca taccaggtcg atgaagacat cacgtttaaa ggtgttacgg aaatcgagcg
    38341 ccagacgggt cacaaaggca acggcttccg gatcatccgc gttaacgtgg aaaatcggtg
    38401 cctgaaccat cttaccgata tcagtacagt acggtgtaga acgggcatcc agcgggttag
    38461 aggtggtgaa accaacctgg ttgttgataa cgatacgtac cgtaccgcca acttcataac
    38521 cacgcgcttt cgacatgttc agagtttcct gaaccacgcc ctgcccggtc actgcggcgt
    38581 caccgtggat ggtgattggc agcactttgt tgctgctcgg ctcatcaagt ctgtccagac
    38641 gggcacgaac agagccgata acgaccgggc ttacaatctc aaggtgagac gggttaaacg
    38701 ccagcgccag gtgcaccagg ccgccatcgg tctggaagtc agacgagaag cccatgtggt
    38761 atttcacgtc gcctgtgccg aggtgttctt tatgtttacc ggcgaactcg tcgaacaagt
    38821 cttgcggttt tttacccagc acgttcacca gcacgttcag acgaccacgg tgcgccatcc
    38881 cgagaaccac ttcgcgggtg ccgctgttgc cagcgtggcg gatcatctct ttaagcatcg
    38941 ggattaacgc gtcaccgcct tccagcgaga agcgttttgc gccagggaat tttgcgccga
    39001 ggtaacgctc aaggccttca gcggcggtca gttcgcttaa gaagcgtttt ttctcttcgc
    39061 tattgaaagt cgcgcgacca gactcgatac gctgttggat ccagcgtttt tcttcggtgc
    39121 tggtgatgtg catatactcg gcaccaatcg ggccgcagta ggtttgcttg agggcggaaa
    39181 taagctcgcc gagtttcatg gtttctttgc cgctggcaaa tgaaccgacg ttgaaggttt
    39241 cctggaagtc tgcttcggtc agatcgtgga aagacggatc cagatcggcc actttatctt
    39301 gctgccacag tcccagcgga tctagattcg catgctggtg accacggaag cggtatgcgt
    39361 taatgagctg caggacttta acctgcttca cattggtgtc agggtcggag atcgttgaag
    39421 agtaacgtga agcgtctttc gccaggcggc ggaaatattc acgcgtttga gagtggaatt
    39481 gatccggttt gactcccgta ccaggtaact gctggaacgt cgaacgccag ttagcgtcaa
    39541 ccgagtcagg atcggttaag aagtcttcat agagctgttc tatccagctc tggtttgcgc
    39601 cagagaggta agaagagtcc aaccaggctt tcaaagcgct gttctgcatc gtgatccctt
    39661 aagcatcttt tttatgctta cttcgccgtg gatactacca cgcacagtga tgtgcgtgcc
    39721 ggggttcact tgcgagctct tgtctacgta gtggctcgcg aaggaacctt taaaaactgt
    39781 cttagtgtca ggggcagttt ttaaagattt cctgcatttc tttgttacgc ctgatgcgct
    39841 gcgcttatca ggcctgcggg taatgacttg taggccggat aaggcgttca cgccgcatcc
    39901 ggcactggtt gcctgatgca acgcttgcgc gtcttatcag gcctacagtt tacgcattac
    39961 gttgcaacaa catcgacttg atatggccga tggcgcgcgt cgggttcagc cccttcggac
    40021 atacactgac gcagttcatg atgctgtgac agcggaatac gctgaatgca tcgctcaaac
    40081 cgtcgaggcg gctgtcagtc tcggtatcac ggctatcgat caggaaacga tatgccgcta
    40141 acaagcctgc cgggccgata aacttatcgg gattccacca gaaagacgga caagaggttg
    40201 aacaacatgc gcagagaata cattcataca gcccgtcgag tttttcgcgc tgctctggca
    40261 tctgtaaatg ctcgcgagct ggcggatttt gtccattatt caacaggtaa ggcttaattt
    40321 tctcatattg cgcatagaat tgtcccatgt ctaccaccaa atcgcggatc accggtaaac
    40381 ctggcagcgg gcgaatcaca atcttcttgc ccggctggtt gagtgccgaa atcggggtaa
    40441 tacaggccag accattcttg ccgttcatgt tcagaccgtc ggaaccgcac acaccttcac
    40501 ggcaggagcg gcggaacgac aggctgggat ctttctcttt cagctggata agcgcatcca
    40561 gcagcatcat gtcgcgacct tcttccgctt ccagggtgta atcctgcata cgcggagcat
    40621 catcaacatc cgggttatag cgataaattg aaaactcgag tctcattttc ctgtctccgc
    40681 attagtaagt acgaatcttc ggcgggaatg ccgggcgcag tttcggttcc atgttgacgc
    40741 ttcggcgcgt catggattcc gactcaggca gatacaggga gtggcacagc cagttttcat
    40801 catcacgatc cgggaagtcg aagcggctat gtgcgccacg gctttcggta cggaagttgg
    40861 cagaaacagc cgttgcatac gccgtttcca tcaggttatc cagttccagg cactcaacgc
    40921 gctgggtgtt gaactcgctg gaagtgtcat ccagacgggc atttttcaga cgctcgcgga
    40981 tcactttcaa ctgctcaagc cctttcgcca tcgcatcacc ttcacggaag accgagaagt
    41041 tatgctgcat acattcttgc agcgctttac ggatcgccac cggatcttca ccgttacgat
    41101 tattgttcca ccggttcagg cgatccagag acgcttcaac atcagactcg ctggcatcgc
    41161 gcagtgcgcc ctgctcggcg atagactctt gcagatgcag acctgccgcg cgaccaaaga
    41221 ccaccaggtc cagcagcgag ttgccgccca gacggttagc gccgtgtacc gatacacaag
    41281 cgatttcacc aacggcaaac agtcccggaa caaccacatc ttcgcctttc tcattcacag
    41341 tcagcgcctg accggtaact ttggtcggaa taccgcccat catgtagtga caggttggga
    41401 taaccggaat cggctctttc accggatcga cgtgagcgaa ggtacgggaa agctccagga
    41461 tacccggcag acgggattcg agaacttctt tacccaggtg atcgagtttc agtttcgcgt
    41521 gtggccccca cggaccatca cagccgcgac cttcacggat ttcaatcatg atggaacgcg
    41581 ccaccacatc acggcccgcc aggtctttgg cgttcggcgc ataacgctcc ataaagcgtt
    41641 cgccatgttt gttcagcaga taaccgcctt caccacggca accttcggtg accagtacgc
    41701 ccgcaccggc aatgccggtc gggtggaatt gccacatttc catatcctgc accggtacgc
    41761 ctgcgcggat agccatgccg acaccgtcgc cagtgttaat gtgggcgtta gtggtggact
    41821 gataaatacg ccctgccccg ccagtcgcca gcacggtagc gcgggctttg aaataaacca
    41881 cttcaccggt ttcgatgcac agtgcggtac aacccaccac cgcgccatcc tggtttttca
    41941 ccagatccag cgcataccac tcggagaaaa tggtggtgtg gtttttcagg ttctgctgat
    42001 aaagcgtgtg caacagtgcg tgaccggtac ggtcagcagc cgccgcagtg cgtgccgcct
    42061 gctcgccgcc gaagtttttc gactgaccgc caaacggacg ttgatagata cgaccatcat
    42121 cgagacgcga gaacggcagg cccatgtgtt cgagttccag aatcgcttcc ggcccggttt
    42181 tacacatata ttcaatcgcg tcctggtcac cgatatagtc cgaccctttt acggtgtcgt
    42241 acatatgcca ttcccagtta tcttcatggg tattacccag cgcaacggta atgccgcctt
    42301 gcgcagaaac ggtatgggaa cgggtcggga agactttaga gagcagcgca caggtctggc
    42361 cgctctggga aatttgcagc gccgcgcgca tacctgcgcc accggcacca atcacaactg
    42421 catcaaattc tctgactggc aatttcatca caccccccac accacaacga atccataaat
    42481 cacgtaaacc accagtgcaa cgacaatcac cagttgcagc atcaggcgca aggccagcgg
    42541 tttaacgtag tcggtcaaca cctgccacat gccgatccag gcatgaatca agatagaaaa
    42601 cagcgccagc agggtgaaca ctttggtgaa cgcagaggcg aagaaaccga tccagacttc
    42661 atatgtcagt tcgccactgg tggcgaaaaa accgaccata taaatgatgt agagcgtcag
    42721 gacgatagcg gtagcgcgaa cgaggatgaa atcatgtacg ccattgcgtc ctaatgcgga
    42781 ggcgttgctt accatacgag gactcctgcg agaagtgaaa gcacgacagt aataacaaag
    42841 gagattttgg cggagcgttt acccgcttcg aatgtttctt ccagatagcc aaaatccatc
    42901 atcatgtggc gaatacctac gacgacgtga tacgccagag cggtaaggat gccccacatg
    42961 ataaatttga cgaagaagct gcccataatc gcggaagctt gctcgaaacc ttcaggggaa
    43021 gagaggctgg tacccagaag ccacagcagg atgcccactg caacaaaggt gatcacaccg
    43081 gaaacgcgat ggagaatgga cgctatcgcc gtgatgggga accggatggt ctgtaggtcc
    43141 agattaacag gtctttgttt tttcacattt cttatcatga ataacgccca catgctgttc
    43201 ttattattcc ctggggacta cgggcacaga ggttaacttt ctgttacctg gagacgtcgg
    43261 gatttccttc ctccggtctg cttgcgggtc agacagcgtc ctttccataa ctgcgcgtca
    43321 tgcaaaacac tgcttccaga tgcgaaaacg acacgttaca acgctgggtg gctcgggatt
    43381 gcagggtgtt ccggagacct ggcggcagta taggctgttc acaaaatcat tacaattaac
    43441 ctacatatag tttgtcgggt tttatcctga acagtgatcc aggtcacgat aacaacattt
    43501 atttaatttt taatcatcta atttgacaat cattcaacaa agttgttaca aacattacca
    43561 ggaaaagcat ataatgcgta aaagttatga agtcggtatt tcacctaaga ttaacttatg
    43621 taacagtgtg gaagtattga ccaattcatt cgggacagtt attagtggta gacaagttta
    43681 ataattcgga ttgctaagta cttgattcgc catttattcg tcatcaatgg attctttacc
    43741 tgcaagcgcc cagagctctg tacccaggtt ttcccctctt tcacagagcg gcgagccaaa
    43801 taacaaacgg gtaaagccag gttgatgtgc gaaggcaaat ttaagttccg gcagtcttac
    43861 gcaataaggc gctaaggaga ccttaaatgg ctgatacaaa agcaaaactc accctcaacg
    43921 gggacacagc tgttgaactg gatgtgctga aaggcacgct gggtcaagat gttattgata
    43981 tccgtactct cggttcaaaa ggtgtgttca cctttgaccc aggcttcact tcaaccgcat
    44041 cctgcgaatc taaaattact tttattgatg gtgatgaagg tattttgctg caccgcggtt
    44101 tcccgatcga tcagctggcg accgattcta actacctaga agtttgttac atcctgctga
    44161 atggtgaaaa accgactcag gaacagtatg acgaatttaa aactacggtg acccgtcata
    44221 ccatgatcca cgagcagatt acccgtctgt tccacgcttt ccgtcgcgac tcacatccaa
    44281 tggcagtcat gtgtggtatt accggcgcgc tggcggcgtt ctatcacgac tcgctggatg
    44341 ttaacaatcc tcgtcatcgt gaaattgccg cgttccgcct gctgtcgaaa atgccgacca
    44401 tggccgcgat gtgttacaag tattccattg gtcagccatt tgtttatccg cgcaacgatc
    44461 tctcctatgc cggtaacttc ctgaatatga tgttctccac gccgtgcgaa ccgtatgaag
    44521 ttaatccgat tctggaacgt gctatggacc gtattctgat cctgcacgct gaccatgaac
    44581 agaacgcctc tacctccacc gtgcgtaccg ctggctcttc gggtgcgaac ccgtttgcct
    44641 gtatcgcagc aggtattgct tcactgtggg gacctgcgca cggtggtgct aacgaagcgg
    44701 cgctgaaaat gctggaagaa attagctccg ttaaacacat tccggaattt gttcgtcgtg
    44761 cgaaagataa aaatgattct ttccgcctga tgggcttcgg tcaccgcgtg tacaaaaatt
    44821 acgacccgcg cgccaccgta atgcgtgaaa cctgccatga agttctgaaa gagctgggca
    44881 ccaaagatga cctgctggaa gtggctatgg agctggaaaa catcgcgctg aacgacccgt
    44941 actttatcga gaagaaactg tacccgaacg tcgatttcta ctctggtatc atcctgaaag
    45001 cgatgggtat tccgtcttcc atgttcaccg tcattttcgc aatggcacgt accgttggct
    45061 ggatcgccca ctggagcgaa atgcacagtt acggtatgaa gattgcccgt ccgcgtcagc
    45121 tgtatacagg atatgaaaaa cgcgacttta aaagcgatat caagcgttaa tggttgattg
    45181 ctaagttgta aatattttaa cccgccgttc aaatggcggg ttaattttta tatgcctaaa
    45241 cacaaaaaat tgtaaaaata aaatccacta tcggacattc atatattcta aaaaaaaata
    45301 tatcggtata aattataaac aaatcgatga tttttaatca tctatcttcc tctcagttac
    45361 atgaataaac caccaaatat agcgcacccc tataatttcc aatactttat tcatcaaaca
    45421 tttaattcat accatcaatt aattactttt acatttaata aaattaataa tatttttaag
    45481 aaaaactaaa aacattcgac aatcaaataa gatattcttg caccgatata cttttttctt
    45541 ttctgttaaa ggactcagtt atgtttaaag gacaaaaaac attggccgca ctggccgtat
    45601 ctctgctgtt cacagcacct gtttatgcgg ctgatgaagg atccggtgaa attcacttta
    45661 aaggtgaagt tattgaagca ccgtgtgaaa tacatcagga tgatattgat aaagaggttg
    45721 aactcggtca ggtgaccacc agccacatta atcagtcaca tcacagcgat gccgttgctg
    45781 tcgacctgct cttagtcaac tgtgatctgg aaaactccag caacggttcc ggtggcaaga
    45841 tttctaaagt tgcagtcacc tttgatagct cagcgaaaac caccggcgca gatccgattc
    45901 tcaacaacac cagcacgggt gaagccacct gcgtcggcgt acgtttaatg aataaagatc
    45961 aaagcaatat cgttttaggc accgctactc cagatatcga cctggctccg acctccagcg
    46021 aacaaacgct gaatttcttt gcctggatgg aacaaattga tcaggctaca cctgtaacgc
    46081 caggcgcagt tacggcgaat gcaacctacg tgctggatta taaataagta ttactttggc
    46141 ggcgctatcg ccgcctttca taaaatattt atcaggtatg gacaccgtga atatttatcg
    46201 actctctttt gtatcctgcc tggtcgtggc gatgccttgc gcattggcgc tcgaattcaa
    46261 tctgaatgtt ctcgataaat caatgcgcga ccgcattgat atttcattat taaaggaaaa
    46321 aggagtcatt gctcccggtg agtattttgt tagcgttgcg gtaaataata accaaatcag
    46381 taacgagcaa aagattaact ggcacaaaaa tgacgataaa accattccgt gcatcaatga
    46441 tttactggtc gataaatttg gcttaaaacc tgaagtccgt cagtcattac cattgataaa
    46501 tcagtgcgtc gattttagct cccgacctga aatgctcttc aatttcgatc aagccaatca
    46561 gcaactgaat attagtattc cgcaagcctg gctggcgtgg tactcagaaa actggacccc
    46621 accctccaca tggaaagaag gtgtcgccgg tgtcctgatg gattacaact tgtttgccag
    46681 cagctaccgc ccacaggatg gcagcagcag cactaatctt aacgcctatg gaacagccgg
    46741 aatcaacacg ggggcatggc gcttacgcag tgattatcag ttgaatcata ctgatagcga
    46801 tgataaccat gaacagtcag gcgaaatatc acgtacctat ctttttcgcc cgttaccgca
    46861 attaggttca aaattaaccc tcggcgaaac ggattttagt tccaatattt tcgacggttt
    46921 ttcttatacc ggcgcggcac tggcaagtga cgatcgaatg ttgccatggg aactacgcgg
    46981 ctacgcccca caaattagcg gtattgcaca gaccaatgcc acggtgacga tcagtcaatc
    47041 aggccgcgtc atttaccaga aaaaagtccc accaggccca tttatcattg acgaccttaa
    47101 tcagtctgtt cagggcacac tggatgtcaa agtgacggaa gaagatggtc gggtgaacaa
    47161 tttccaggtt tcagcagcat cgacgccctt cctgactcgt cagggacagg ttcgctataa
    47221 actggccgcg ggtcagccac ggccctccat gtcacatcaa actgaaaatg aaaccttttt
    47281 tagcaatgaa gtttcctggg ggatgctgtc aaacacctcg ctgtacagcg gcctgctgct
    47341 ttctggtgat gactaccatt ctgccgcaat gggtattggg caaaatatgc tgtggcttgg
    47401 tgcgctgtcg tttgatgtca cgtgggccag tagccatttt gatactcagc aggacgagcg
    47461 gggcttaagc taccgtttta attacagcaa acaagtggat gccactaaca gcacgatttc
    47521 cctcgccgct tatcgtttct ccgatcgtca ttttcacagc tacgccaact atctggatca
    47581 caaatacaac gacagcgatg cgcaggacga aaaacagacg atcagcttat ctgtgggcca
    47641 accgattacc ccactaaacc tcaatcttta cgccaacctg ctacatcaaa cctggtggaa
    47701 tgcagacgcc tccacgaccg ccaacatcac ggcaggtttt aatgttgata ttggtgactg
    47761 gagagatatc tctatttcta cgtcattcaa tacaacccat tacgaagata aagatcgcga
    47821 caaccagatt tacctgtcga tttcgctccc cttcggtaac ggtggtcagg ttggctatga
    47881 catgcaaaac agtagccaca gcaccacaca ccgcatgtcg tggaacgata cgctggatga
    47941 acgtaatagc tggggcatgt ctgccggact gcaatccgac cgtcctgaca atggagccca
    48001 ggtgagcggt aactatcagc acctgagttc agcgggtgag tgggatattt ctggtaccta
    48061 tgccgccaat gattacagtt ccgtcagcag cagctggagc ggttctttca ccgcaaccca
    48121 atatggtgca gcgtttcatc gccgcagctc caccaatgaa ccacgcctga tggtcagcac
    48181 cgatggcgtg gcagatattc cggttcaggg caatctcgac tacaccaacc attttggcat
    48241 tgcggtggtg ccgttgattt ccagttacca gccttccacc gtggcggtga acatgaatga
    48301 cttacccgac ggcgtaacag ttgcagaaaa cgttatcaaa gaaacgtgga ttgaaggcgc
    48361 gataggttac aaatcactgg cttcccgttc cggtaaagac gttaacgtca tcattcgcaa
    48421 cgccagcggt cagtttcctc ccctcggagc ggatatccgc caggatgaca gcggcattag
    48481 cgtggggatg gttggcgagg aaggacatgc ctggttaagc ggagtcgctg aaaatcaaaa
    48541 gtttaccgtg gtctggggtg atagccagca ttgctcgctc catcttcctg aacatatgga
    48601 agacaccgca aatcgcctga ttttaccttg tcattaatta aggaaatgaa aatgacattt
    48661 atgaaaggac tacccctttt gttgttggtt gccagtctgt gcagccacgc tgcactacaa
    48721 cccgatcgca ctcgtattgt gtttaacgct aatgataaag ctaccagtct gcgggtggat
    48781 aatcgtagcg ataaactccc ctacctcgct tattcctggc tcgaaaatga aaaaggggag
    48841 aaaagcgatg atcttctggt cgccctgccg ccgatacaac gtctggaacc gaaagcaaca
    48901 acgcaggtac ggatagtaaa gcaagcctcg accacaaaat taccgggcga tcgcgaaacg
    48961 ctatttttct acaatatgcg tgaaattcct cccgccccgg aaaaaaacag cgaccatgcc
    49021 gttctgcaag tcgccattca gagtcgtatc aaggtgttct ggcgaccggc tgcattacgc
    49081 aagaaagcag gagaaaaggt cgaactgcag ttacaggtca gccagcaggg caaccaactg
    49141 acactgaaaa atcctaccgc gtattatctg accattgcct acctgggacg aaatgaaaaa
    49201 ggcgttctcc ccggcttcaa aaccgtgatg gttgcgcctt ttagtacggt caacacaaac
    49261 accggaaatt atagcggcag ccagttttat ctcggttata tggatgatta cggcgcgtta
    49321 cgcatgacga cgctcaactg cagcggacaa tgccatttac aagccgtgga ggcgaagaaa
    49381 tgagtgctgg caagggagtg ttgctcgtca tctgtctgtt atttctgccg ttgaagtctg
    49441 cgcttgccct gaactgctac tttggttcat ccggtggctc ggtagaaaaa tcagaagcga
    49501 ttcaaccttt tgctgtacca ggcaatgcca aactcggcga taagatctgg gaatctgacg
    49561 atattaaaat tcccgtctat tgtgacaaca ataccaacgg caactttgaa agcgaacatg
    49621 tctacgcatg ggtaaacccc tacccggggg tgcaggatcg ttattatcaa ctcggagtaa
    49681 cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac actaaccagt
    49741 gtatcgacag taagaacatt gacatttata cccttgagca gatcatcgcg atgggatggc
    49801 agaacaaaat ttgctcgggt gatcccgcta atattcacat gtcgcgtacc tttctcgctc
    49861 gcatgcggtt atacgtcaaa atacgagaaa tgccgccgca tgattatcaa agtacgctta
    49921 gcgactatat cgtcgtgcaa tttgacggtg ccggtagcgt taacgaagac cctactgccc
    49981 aaaacctgaa atatcatatt actggtctgg aaaacattcg cgtgctggat tgcagcgtca
    50041 atttttccat ttcaccggaa acacaggtga ttgattttgg taaatttaat ttgctggata
    50101 ttcgtcgcca cacaatgtcg aaaacattca gtattaagac gacaaaatca caaaatgacc
    50161 aatgcaccga cggatttaaa gtcagttcct ccttttatac cgaagaaacg ttggttgaag
    50221 aagataaagc attgttgatt ggtaatggtc tgaagctacg tttgctggat gaaaacgcct
    50281 cgccttatac cttcaataaa tatgctgaat acgccgattt caccagcgac atgttggtct
    50341 acgaaaaaac ctatacagct gaactttcgt ccattgcagg tacccccatc gaggctgggg
    50401 cccttcgata cagtggtgct ttttaaaatt aactataact gatttgccat gatgcccttt
    50461 gcagccggaa agggcatctc tttcagaaaa ttgctgattt tccccttttc cttgattgct
    50521 gttatttttt cctagcccgg ctttctttat gctgtatcga tacaccaaag agaaaataat
    50581 gagggagcga aggatgccag ttttgcagtg gggaatgtta tgtgtgctat cacttctcct
    50641 ttctattggt ttcctcgcgg tacatctccc ggcggcgcta ttacttgggc cgatgatcgc
    50701 cgggatcatc ttcagtatgc gcggaattac tctgcaactt ccccgctccg cttttctcgc
    50761 cgcgcaggcc attcttggct gcatgattgc gcaaaacctc actggttcga ttctcaccac
    50821 gctggccgcc aactggccga tcgtgcttgc tattttactg gtgacgctgc tttccagcgc
    50881 catcgtgggt tggttattgg tgcgctatag ctcactgcct ggaaataccg gtgcctgggg
    50941 ttcttctcca ggcggcgcag cggcaatggt cgctatggcg caagattacg gcgcagatat
    51001 tcgcctggtg gcgtttatgc aatatctgcg agtgctgttc gtcgcgggtg ccgctgttct
    51061 ggtcacccgc atgatgctgg gtgataacgc tgaagcggtt aaccagcaaa tcgtctggtt
    51121 cccgccagtg agcattaatc tcctgcttac gattttgctc gcagtcgttg ccggtacggc
    51181 gggatgcatg ctgcgtcttc cttccggcac gatgctcatc ccgatgctgg cgggcgcagt
    51241 gctccagtct ggtcagctca tcaccatcga actaccggaa tggctgctgg cgatggcgta
    51301 tatggcaatt ggctggcgga ttggtcttgg tttcgataaa caaatcttac tgcgggcact
    51361 acgcccgcta ccgcaaatcc tgctgtcgat ttttgctctg ctggctattt gtgcaggtat
    51421 ggcgtgggga ctgacccggt ttatgcatat tgattttatg actgcctacc tcgccaccag
    51481 tcccggcggg cttgataccg tggcggtcat cgccgcaggg agcaatgccg atatggcgct
    51541 tattatggcg atgcaaaccc tgcgcctgtt cagtattttg ctgacagggc cagccattgc
    51601 gcggtttatt tcaacctatg cgccgaagcg gtcggcctag tgctggcagc ctggacacca
    51661 gtaaaacggg cgcgaggaca acgtggtttt ctcaatgatg ccgccacagc gttcgcacag
    51721 ttcgccatca cgatgaaaaa ccttaaagcg aaacagcgcc ccatgatgct tattctcatc
    51781 cacctgcccc cgcgtagcgt aagaaaaccg aggaatctcc agtaacgcgt gtgcgagtgc
    51841 atccagttgc gccgcattga gatctttcgc tttatgattt ccagtcaacc caacctgcca
    51901 gaggatctcc acccgcaaat aattgccaag cccagccaga aacgcctgat cgagcagtaa
    51961 tcctgcaaac tgacggttac gaaagcgcgg cgacaataac cgttccttta ccacctccgg
    52021 cgtcagattc ggatccagca catcgggacc aacgcgttgt aaaaacggat gcgtggtcag
    52081 ttgttccggc ctcaacatct caatatccga ggcgctataa agcagaatgg ttttgtcagc
    52141 cgtttgcagt tttacccgca atactcgcgt ggtctgcggc tcttcgccgg tatcaaccac
    52201 gcgccagacg ccgtaaagct gattatggct gtagagcgtt aaaccgttgg gaaaatgagt
    52261 taacaacgcc ttaccacgcg tttccacatg ggtaacgtgt tgaccgataa gttgtgattg
    52321 ataagttttt aactgcggga aggcaaacca gacatcagtt agtggtttgc ctttgatcgc
    52381 cgcctccagg ttatccgctg cacggcggat ctccgggcct tcaggcatgg tgatatcctt
    52441 ttttcattct ttattaagcg gggttatgct gcgacaacaa tccccttttt ggcaaatgta
    52501 gctcgtaatc ggcgggcgaa cgccagagcg tgctcgccgt cgccatgcag gcagaccgtt
    52561 tgcgccgcga ccgttgccca ttcgccggtg atacttttga ctctgccgtg ttgcaccatc
    52621 tccagcgttt gcgccaacgc ctgttcttcg ttttcaatca acgcgcctgg ctgacttcgc
    52681 ggcaccagcg agccgtcagc ctgataaccg cgatcggcaa acacttcctc gcgcgttgtc
    52741 aggccatatt gcttgcctgc acgaatcagc tcgcttcctg ccagcccaac gagaaccaat
    52801 gccggatcgc aagcgtatac cgctctggcg atggcgtctg ccagttgtgc ttctttcgcc
    52861 gcctggttgt acaacatgcc gtgcggtttg acatgacgca ttacgccgcc ttgcgcacgg
    52921 gcaatcgttg ccagcgcgcc aatttgatac agcgtctggg cgtaaacggt ttccggcggc
    52981 agctgcatgg cgctgcgacc aaaattttcc ctgtcgggaa aactcgggtg agcgccaatc
    53041 gcgacaccat tttttattgc ttcacgcacg caagcctgca tggtttgcgc atcgcctgca
    53101 tgaaatccac aagcaatatt ggcagaggaa accagcgtta atagctctgc gtcgctggcg
    53161 cagccttcgc ccagatcggc gttcaggtca attttcattg tgcagccgcc acgctaattg
    53221 ttcaaaataa cgttgctgat cttgccgcgc tttcagtgcc tcttccagtg aacactggac
    53281 aaaatgaatc ggctgaccga gcggaatttg cgccagatgg tacatatcag cctcaatgat
    53341 acaggcaata cgtggataac caccggtggt ctgtgcgtcg ttcatcaaca caatcggctg
    53401 cccgttatgc ggcacctgca ccacgcccgg taacaaaccg tgagataaca gttcgcgatc
    53461 ggtgatgcgt tttaaaattt gcccctgtaa acgatagccc attcggttac tttgcgagct
    53521 aagctgccag ggcgaacgcc agaatgcatc ctgcgaggcg cgatcgaact catgatattc
    53581 cggccccggc aaggcgcgaa tgcggttgcc ccacagcagc tgtttaacgc cctgcgcttc
    53641 cataaaatca cgcttcgatt tgccaatcgg gagtcggtca ccatccttca gtaaacggcc
    53701 ttccagcccg ccaatcccca ctttgagatc ggtgctgcat gaccccatta ccggcggaac
    53761 atcaatacca cccgcgaccg ccagataact gcgcatcccg tgctgcgggc gtttaagcgt
    53821 taaacgctgg cctgctttca tcggcaatcg ccagccggtc cagacggcgt tatcatccaa
    53881 ccgcgcttcg caacctgcac ccgtcagggc aaaccaccca tcagtttcga actcaactgt
    53941 taactgaccg agcgtgatct ccagcgcggg ggcattggcg tcattaccca ccagcaggtt
    54001 agcaatgcgc agcgcaggca tatccagtgc gccacagtgg ctgatacccg actggcgaaa
    54061 accgtgacga ccgccatcct gcacagtggt gtacatgccc gcacgaataa tcttcagcat
    54121 attccctcct tctgcggtac aaatcgcacg ctgtctcccg gacgtaataa gatgggttcg
    54181 tcacgcgccg gatcaaacag gctgagtgag gtatgaccaa tcaactgcca gccacccggc
    54241 gttgccagcg gataaacacc agtctgcggc ccgccgatcc cgacagaacc tgccggaacg
    54301 agcaagcgcg gttcagcgcg ccgtggcgtg tgttgttgtt ccggcaaact cccgagatac
    54361 gggaagcccg gttgaaaacc taaaaaccag accacgtatt ccacggagga gtgcaattca
    54421 acaacctgtt tttcgctcaa cccgcaatac gccgcgacca ccgccaaatc cggtccgcct
    54481 gcaccaccgt aaaccaccgg aatttcaata aagcgagact caggttccag ggcctcgctc
    54541 tcctcccacc agcgttgcaa acgctctatg gcatccagcg ccagcgactc aggattacgc
    54601 aaaatcaccg tgatattgtt catgccggga atggcttcaa ccacattcgg catatccacc
    54661 agacgctgcg ccagtcgcca gatccgcttc tggctagcca gcgtcaccgg cggttccagt
    54721 tccagcacta ccgccgtttc acctatcaga taacaacgcg ctcgttgcac tttcgatccc
    54781 tctgattatt cgttatgcag gattaggaat atcaataaag gtcacatcaa gatcggtatt
    54841 ttcattcagc cactcgctca atgcgcgaat accaccgcgt tcagtggcat ggtgacctgc
    54901 agcataaaaa tgcaatccct gctcgcgggc tgaatgaatg gtctgttcag aaacttcgcc
    54961 agtaataaaa gcatccacgc caaaacgcgc ggcgctatcg ataaaacttt gcccgccgcc
    55021 cgtgcaccag gcgacgcgct ggaccacctc aggtccggta tcgccacacc ataacggctt
    55081 acgtcccaga cgcgcttcaa tccaggaggc tagttccatt cccggcacag gcatggtcag
    55141 ctctccccac ggcaccaacg gctcaatttc gcccatgacc gtgatcccca gtaacgccgc
    55201 cagctgtgcg ttattgccca gctcaggatg tgcgtcaagc ggcaaatgcc agccatacag
    55261 gttgatatca ttcgccagca acgtttttaa acggttacgc ttcattccgc gaatgaccgg
    55321 agactctcct ttccagaagt agccatgatg cacaatgacc gcatccgcgc ccagacgcac
    55381 tgcctcatcg agcaaagcct ggctggcggt gacaccggta acaatttttt gcaccgtctc
    55441 tttgccttcc acctgcaaac cattcggcgc gtaatcacta atcgccgcgc tgttcagttt
    55501 ttcgttaatc agttgttcca gttcggtgtt tttcatcttc gcgccttccg tattctgata
    55561 gctacaccgt taacatagcg ttacctcgcc tgttcgtcga tagccaatct tttgacatat
    55621 taagaaacaa acccgacgcc aaaagacgca aattgtttat atcatcacgc ttcagctaat
    55681 gtatatttat gcaatcagac agcatattgc ataaataatc agttctgtat caaagtttta
    55741 actttaagga aacacaagca cgccgcgcaa ctattacgtg acgttgccgt caattgtgga
    55801 attattatga ataaacacgc atcacaaccg cgcgctattt actatgtcgt tgcgctgcaa
    55861 atctgggaat attttagctt ttacggcatg cgtgccctgc tgattctcta tctcaccaat
    55921 caactaaaat acaacgatac tcacgcctac gagttattta gcgcctactg ttcgctggtg
    55981 tatgtcacgc caatcctcgg tggctttttg gcggataaag ttctcggcaa tcgcatggcg
    56041 gtgatgctgg gggcgttgtt gatggcgatc ggtcatgtgg tgctgggtgc cagtgagatc
    56101 catccgtcat tcctctatct gtccctggcg attatcgtct gcggctatgg cctgtttaaa
    56161 tctaacgtaa gctgtctgct cggcgagctg tatgagccaa ccgatccgcg tcgtgatggc
    56221 ggtttctcgc tgatgtatgc ggcgggtaac gtggggtcta ttatcgcacc tatcgcctgt
    56281 ggttacgccc aggaagagta cagctgggcg atgggctttg gcctggcggc ggttggcatg
    56341 atcgcgggtc tggtcatttt cttatgtggc aatcgtcatt tcactcatac ccgcggcgtt
    56401 aacaaaaaag tactgcgtgc gacaaacttt ctcctgccga actggggatg gctgctggtt
    56461 ctgctggtgg caacgcctgc actgattacc gtgctgttct ggaaagagtg gtcggtatac
    56521 gccttaattg tcgcgactat tattggcctg ggtgtactgg caaaaattta tcgcaaagca
    56581 gaaaaccaga aacagcggaa ggagctgggg ctgattgtga cgctcacctt attcagtatg
    56641 ttgttctggg ccttcgcaca acagggcggt agctcgatta gcctttatat cgaccgcttc
    56701 gttaaccgcg atatgtttgg ttataccgtt ccgaccgcga tgttccagtc gattaatgcc
    56761 ttcgcagtta tgctgtgcgg tgtgttcctg gcgtgggtgg ttaaagaaag cgtcgcaggt
    56821 aatcgtaccg tgcgcatctg gggaaaattt gctcttggcc ttggcctgat gagcgccgga
    56881 ttctgcattc tgaccttaag cgcccgctgg tccgcaatgt atggtcactc ttctctgcca
    56941 ctgatggtgt taggcctcgc ggtgatgggc tttgcggaac tgtttatcga cccggttgcc
    57001 atgtcgcaaa ttacgcgcat tgaaattccg ggcgtgaccg gcgtattaac cggcatctac
    57061 atgctgcttt ctggcgcgat cgcgaactat ctggcgggcg tgattgccga tcagacatcg
    57121 caggcttcgt ttgatgcttc cggggcgatc aactactcca tcaatgcata tattgaagta
    57181 tttgatcaaa ttacctgggg cgcactggcg tgtgtaggac tggtactgat gatttggctg
    57241 tatcaggcgc tgaaattcag aaaccgcgcg ctggcgctgg agtcataata ggccagcacc
    57301 gatgcctgat gcgacgcttg acgcgtctta tcaggcctac gtatttcctg caatttattg
    57361 aatttgcaca aatttgtagg ctggattagg cgttcacgcc gcatccggca tctgggctcg
    57421 attgcctgac gcgtctgtta tttccccttc cgcgccgtct catacgctgc caacgtttgt
    57481 actctcgcct ctttgtgctc gactatcggt tgcggataat ccagcttcac acctgctttc
    57541 tgcgcccact tccacggctc atgcaccgct ttccctggta catcgcgcag ctccggtaac
    57601 catcgacgaa taaactcgcc ctcacggtca aatttctcgc cctgagttat aggattgaaa
    57661 atacgaaaat acggcgcagc atcagtaccc gtagaagccg cccactgcca gccaccgtta
    57721 ttagctgcca aatcaccatc aatcagctgc gacataaaat atcgctcgcc ttcgcgccag
    57781 tcgatcaaca aatctttcac cagaaaactg gctgtaatca tccgtagccg gttatgcatc
    57841 cagccagtgc tgttaagctg acgcatagcg gcatcaataa tcgggtatcc cgttttgcct
    57901 ttctgccagg cctgtaaatg tgcgggattg ctttgccact gcacacgatc cgtccaggca
    57961 ataaatggac gatgtttaca caacgagggg taatacgtca tcagatgacg gtagaactcg
    58021 cgccagatca gctcactaag ccagacacta ccggccccac cgtccagcgc ctgcggctgt
    58081 tccgccaaca agcgatgcaa acactggcga ggcgataacc cgcccgttgc caagctggcg
    58141 gacaaacggc tggtgccttc cactgccgga aaatctcgtt gttgctcata ctctccagca
    58201 ccgttctcgc aaaactggcg taattgcgca atcgccgctt tttcttccac cgggaaatgc
    58261 gcagtatcga aagactgacg aggataattc agcgtaatgg atggcgcggg ctttatcgat
    58321 ccgctactac gaacttttgg tgcagcgacg cactccggca tcccttcccg cagccgtttc
    58381 agccaggcat tcttaaaagg cgtaaagact ttgtacatct cgtgattgcc ggtcatcacc
    58441 gcccccggcg gcaggatcac gctgtcatca aatccttcac acaccacgtt acgcagcgct
    58501 ctttcaacgt ccacatcccg cgctcgctca ttcacctcat actgatagtt ataaaaaaga
    58561 tgagtaacgc tgttttccgc gcacacctgt ttaactattt cgacactggc gacaaagtca
    58621 tccacttcac ggaacaataa aggaatacct ttttccgcca gcgcgatttg tagcccattc
    58681 agttgagcat tgataagttc agcctgacgc ggcgacatgt tatgcgtctc ccactggcgt
    58741 ggtgtagcga tatacagcgc cagcacgcgt gcagacgaat tgcggcaggc ggcagccagt
    58801 gcgagattat cgtgcagacg taaatcctgg cgaaaccagg ccagatgggt agtcataaaa
    58861 ctcctgaatg gcgcaagtta atcacacgcg gccaaagttc gaaataacgc tgccctgaaa
    58921 gccaggcgtc aggataaagc gccatatagt gtttgatacg catcagcggc gcaagaagtg
    58981 gttgctcgcc acggcgataa ctgtcgataa gcgcagccag ctgctggcgt tctgtggaat
    59041 caatatgcgg gcgaaaataa ccctgaacgt gcatcaaaac gttggtgtga tcgcgcgggt
    59101 tggcgggatg agaaagcaaa acaattacgc gttggcggta ctggttgtaa aaatcatcca
    59161 gattttgcca ctgatgaata tcagccacaa acgggccaag tttgcggtac tcgggctgag
    59221 aatgggccag aaagacgagt ttatagcggc tgtgataatc gagaagcgcc ccgcgcgtta
    59281 agccatgttc tttcagtcga tgtaactcat gaagggcgaa gatacggatt agggtgctgt
    59341 catcaaatcg ttgtagattc attactgctc ctgaacggta tacaggagta agtgtagacg
    59401 ttggtaaatg atgtttgtga agttaatccg ctagcagggc aagattacga aagcgcgctc
    59461 cccgcaagca actaatcaag gtagttaatc atggctgaac acgatattac gccagaagtc
    59521 accatttcca aaacacagcg ccgctataaa gtcggctatg tcagagcccg ccaccaggac
    59581 cgcaaaaccg gtatagcccg gtattatagc ctgcacctta agggcgactg gctgaaagag
    59641 gccggatttg ataccggacg gggtgtcacg gtgaagattt cggagggggg cctgaccatc
    59701 atcgctgaca gtgacgaggt gcaggaactg cgggaggaac tttatcaggt taaacaggta
    59761 gttaagggga tgcgggatgg gatgtttagt gtgctgaacg aaagttaaat attaaaaaag
    59821 ctgggaagat ttaaaacctt cctagctttt tattctgtct tattataatg tatactcatc
    59881 atcttccgat gaatttataa attgctccaa gtttacatga agagtactta acctagtttt
    59941 taactcatta agtgaagcga taatttcata ttttccaaca taatcatatg atggatcgta
    60001 ttcattatct atgtcagaag ctaaaaatga tgatattcgt agtttaaatt tagcttcatc
    60061 tttattacat tcattggcat aaggtgaatg ccctatcata gcatattcct ctaatctaaa
    60121 aggataagat ggttttaatt catcgatcaa tgaaatttca atccatgttc tatatccagg
    60181 cattaatgga ctaatttctt tgagcaaaac ctttccactt aataatgtat tgagattgtt
    60241 cattattatg tctccctcgg gaaaaatata tgtctaacac ttccaccatg ttgatgaact
    60301 tgtaaatgtg gtaaatcacc atcaggattt cctggtccat gacctgacac tctatatgga
    60361 ggttttgtta cagaaggatc ttttatatca tcccaatgat atgaacctgc ttttgtttta
    60421 tttccagtaa gatattccat taagttctta gtgctggggc ctgactcacc tgtcatattt
    60481 ttataattat gacctaaata tctatccata aacaattgtt cagcgtcact tctagaccta
    60541 acagtaactt tggtttcact actatctaat aaacgattcg ctttttggtt actaacagca
    60601 catggagtta atccaagtgg atcagcatat tttagaggat taggtgcata actgtagaga
    60661 tttaaaccgc ctctcaaccc tatcgggtca gttaccatga accgcccgac atccggatca
    60721 tagtacctgt gcagattgta gtgcagcccc gtttccctgt cgaggtactg tccctggtag
    60781 cgcaggttct gctccacccc gccgtggtcc ttctcctgta tcgtgttacc ccagacctga
    60841 taccccgtct cccacacgat tttcccgtcg ctgtccgtca tctcttccgg cgcgccgttc
    60901 acatccgtgt gaaaatacag tatccggttc gggccctctt tgcccgcctt atccacccgc
    60961 gccagcggtt cataactccc ctggtccctg tagatgtaca ggctgcctgt tccctccggc
    61021 ctgctctcct gcgccagccg cattccgtcc cataaaaatt ttgtccggtt gtacggctta
    61081 ccgtcagggc tgatgtgctg tttttccgtc cgcctcccca gtgcatcgta cacataccgg
    61141 tatgtccccc gcgctgtcgt cacttccacc atccggtgtt ccgcatcata acggtaactc
    61201 tgcgttccgc tccggccctc cttttccacc gcacggccaa attcgtcata acggtagtgc
    61261 acaccacggt aagacatcag ccggttgaac ggcaccacgc tgttttctgc cgtggccctt
    61321 tcccccgcgc gcctccccag caggtttccc gccgcgtcgt actgccagct cgccagatac
    61381 ccctcatcct ccgaacgaca cgccgtgatg ctccccgtgg ggtcgtagaa gtaatccgtt
    61441 tccccccgcc ggctgtgccg cgtcttcagc agttcatcct cgccactgta gctgtactgc
    61501 cggtccgtga acgtctccgg taccaccccg cgcatcccgc tgtatgtctc cctgcgcttc
    61561 agccgcccgc cggggtcgta aagctgccgc gtgctcagtc gcccctggct tctgagcact
    61621 tcacggtgca ggtggtcgcg cgtgaactcg ctgatgacct cccggtccag atttatctgc
    61681 tggagatggc cgctgccgta gtacagatgg cgctgcgttc tgccgtccgg aaaggtggtg
    61741 gcagtccggt tgcccagttc atcgtactgg tgggtcagga tgccctgcgc cgtctcctcg
    61801 ctcagcatcc gccccagtct gtcgtagctg aagcggataa cttccggcgc gtcagtttcg
    61861 tgccgctggc gacggatttc cagtagctgg tctgccgcat catggacgta ttccgtacgg
    61921 ccatcggctg tctcttttgc cgtgagccgg cccgccgcat cacgcgccag ccggtggcgg
    61981 atttcaccgc cccggtcccc ggtgaacacc acttccgtga ccgcgttcag cgcgttgtag
    62041 cggtacgact ggcggcagcc gtccgggttt atctgctccg ccagacggtc cagcacatca
    62101 tggcggaacc agtagcgttc tccgttctcg ttcgtgagcg tgaccagacg cccgtaagcg
    62161 tcgtacccga atgccgtcct gcgccccatc gcgtccgtcc ggctgcgcac ccggccctgc
    62221 ccgtcccgct gccagcgcgt tatcctcccc atcgggctgg tgtactttac cagcctgccc
    62281 gccgcgtctg actggtaatt caccgtgcgg ccgtccgcca gtatcacctc cgtcagatga
    62341 ccgctgtcgt catacccgta gcgcgtgctg tggctctgcg catccgtcat tctgataagc
    62401 ctcgtgcgtt cgtcgtagaa ccacgccgtc tgcgagccgg aacagtccgt gtgccgcatg
    62461 agctgcccgt cttcattcca ctggaggtat ttattccccc cgcgcgcatc tgttatctgc
    62521 accacctggc cgtggtggtc atactgatac cgggtgttct gctgcagcgg gtcggtcacg
    62581 gcaagaaggt tgccgcgttc atcgtacaca cagcgccagg tgtttcccgc cgcgtccacc
    62641 tccgtttccg gctggtgcca caccgggtgc cactgcgcct gctccacccg ccccagcggg
    62701 tcgtgcgtct ccgtgatatg cccctgcagg tcatacacat agcgccattt gccgccctga
    62761 gggtcggtca tccccagcaa cagtcgttct tcgtcgctcc agcggaatgt cgtcacctgt
    62821 ccggcttcgt cctggtacgc cgtcacctga ttctgcgcat cccactggtg ataacgtgtg
    62881 cgccccagtc cgtccgtcac gcacagcctg cgctgcgcca ggtcccacgc cagccggtac
    62941 tgttcaccgc tgtccgtgtg gtgccccgtc acccgccatg ccgcgtcatc cggaccttca
    63001 taccagcccc actggtaata atacccttcg ccgcccggct ggcggtgcag cgtcatgcag
    63061 tggttttcgt cgtaagcaaa ctcacgcagc aggtgccccg tgctgtcgta cacgccgttc
    63121 aggtggccgg cctcgtcata gctgtaacga cgcagcagta aaggtgcagt ctcagggaaa
    63181 tgctgataaa tctgtgtcac ccgccgggga tgcgccgcca gttcgtaata cagacggatg
    63241 cacggacgct gctgttcgcc gctgatttcc acaatgcggc cccggtcgtc gtagtacaga
    63301 ttcagacagt tgctgttacg gtcgcccagc attttcagcc gctggcgctg cgggtggtgc
    63361 gggtcagcct caaacagaaa acactgcccg tcatcgctgc tgataagcca gtgcccctgc
    63421 tcaccacgcc ttaccgccag cccttcaccg gggctgtaaa acccgccccc cggcagtacg
    63481 gcttccatat ccaggcgtcg ccccatgccc gacacaaagg tcatgcagtt ttcgtctgcg
    63541 ctacccggtg tgcacacgag gctgacttca tacattacac tccagccctg accaaacatt
    63601 ccttccgtac gttcgtcacg actgctgtac acgcgctgcc agtccagcgg aaagtggccc
    63661 ggcagactga aatcgaagtc atcgtcatcg gccaggtatt tggccccggt ggcggcattg
    63721 accggatgcg gaagggcact ggtggccttc tgcgtcagca tgctcatccc gacactggca
    63781 gcaaaacagg cgattttaga aagcattttg cccgggcgaa gaaacatcag cgccacggtt
    63841 gccaccaggg ttatctgcga cctgccgctt ctgatatccc tgaccaccag taacggaccg
    63901 ccaatgcgca catccggtga cacaaacgcc ccgttccccg gctcattcac caccctggcc
    63961 tcgcaggtac agcgtgcccc gctgcgtgtc gccggctggc tgttgatacg cacatcccgc
    64021 gcgccttccg cgatatattc cgctgcgggc ggctcgtcat cgcctttgaa cagattactg
    64081 atgctgccaa taaccgcatt cactccctga aaggcggttt ccagcatacc gcccggattc
    64141 agcaggttaa gggccgttcc cagggggtca tccagaaagg cggctttttt ttgtgcgaga
    64201 aactgcgtca gtgaggaagg atgtttatgg cagtcgattt tatcgtcagg ggtcggggtg
    64261 gagcccgggg ccgcactggc cacagtgggc tgccatagtt ccgacacaaa atgtgtcacg
    64321 ccatccacca tttcagaacg cccttcttcc gtactgagct ggtcgtgcag agcccgggac
    64381 atcagcacag gccctgtcac cgggttaacc gccagggcta tggcccccgc catttccaga
    64441 tattccccgg ccacatcccg gaacgttctg gcatcctcct gacgctgcgc ttcttcctgt
    64501 tctgccttcg cctgcgcttc ctgcgcctct atgtcctgcc gcgatgccgc agtcgccgct
    64561 gcccgcgcgg caggtttgct gttgatccat gtgtcaccag agccggtgag gatgtaacct
    64621 tcaatctgag gcgggaaaag catatttgcg agggctgaac aggcttcgct gaccttttct
    64681 gtcacgccgg tgtacaccat cgccacaccc gccacaaaac cggtcagcac gacggccgtg
    64741 ccccccatcg tgatccccac tgacgtcgcc agtaacgagg ttgtgccgac aatgagggca
    64801 tcaataacga catttgcagc cacctccagc accgccccca gcacatctgc cataaacgac
    64861 gtatgcagca gggcatcccc ctgtcttgcc gcccagaatg cctcttccat gtcccgttcc
    64921 ctgtatgatt aattcgtcct gacttcctgc cgcatcctga agcgcagatt ctttttaatt
    64981 tcagcccagc gcgtcatatc tgccggggtc agcggtgtgg ttctggcgta ggtgaaggcc
    65041 atcatgaccc gctcacccgg caatacaaat gccacctgac actggtacac ctgctcgtta
    65101 ccccggagaa actggctctg tatttccaaa ccgcctcgtt gagataatat tccgtcataa
    65161 tgtaaatagt ccgttttaat aaaggaatta tttattgaca ggttctgttt ttttaatcat
    65221 gaatgtaata cccatcaccg ctttacagca cactttatct ttccagccat aatcactaat
    65281 aatattaagc aattagcaat atttacaacg accagagcgg atacatagtt atgtatttaa
    65341 caatcccaat atattcacta tgcaaataaa accactagta acagacacgc taccgcctcc
    65401 atataaaaat ggacacctt
//
LOCUS       DAJTNM010000010        60413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTNM010000010 DAJTNM010000000
VERSION     DAJTNM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.010"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            173..967
                     /gene="panB"
                     /locus_tag="OO172_000733"
     CDS             173..967
                     /gene="panB"
                     /locus_tag="OO172_000733"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5511018.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            979..1830
                     /gene="panC"
                     /locus_tag="OO172_000734"
     CDS             979..1830
                     /gene="panC"
                     /locus_tag="OO172_000734"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5511019.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(1904..2806)
                     /gene="rpnC"
                     /locus_tag="OO172_000735"
     CDS             complement(1904..2806)
                     /gene="rpnC"
                     /locus_tag="OO172_000735"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5511020.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3080..3460
                     /gene="panD"
                     /locus_tag="OO172_000736"
     CDS             3080..3460
                     /gene="panD"
                     /locus_tag="OO172_000736"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5511021.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3464..4693)
                     /locus_tag="OO172_000737"
     CDS             complement(3464..4693)
                     /locus_tag="OO172_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5511022.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(4757..5197)
                     /locus_tag="OO172_000738"
     CDS             complement(4757..5197)
                     /locus_tag="OO172_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5511023.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5302..6072)
                     /locus_tag="OO172_000739"
     CDS             complement(5302..6072)
                     /locus_tag="OO172_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5511024.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6069..6995)
                     /locus_tag="OO172_000740"
     CDS             complement(6069..6995)
                     /locus_tag="OO172_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5511025.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7104..7766
                     /gene="can"
                     /locus_tag="OO172_000741"
     CDS             7104..7766
                     /gene="can"
                     /locus_tag="OO172_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5511026.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(7807..8343)
                     /gene="hpt"
                     /locus_tag="OO172_000742"
     CDS             complement(7807..8343)
                     /gene="hpt"
                     /locus_tag="OO172_000742"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5511027.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8549..10939
                     /gene="gcd"
                     /locus_tag="OO172_000743"
     CDS             8549..10939
                     /gene="gcd"
                     /locus_tag="OO172_000743"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5511028.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHAFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFITATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(10986..12535)
                     /gene="cueO"
                     /locus_tag="OO172_000744"
                     /pseudo
     CDS             complement(10986..12535)
                     /gene="cueO"
                     /locus_tag="OO172_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12701..13048
                     /locus_tag="OO172_000745"
     CDS             12701..13048
                     /locus_tag="OO172_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5511029.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13154..14020
                     /gene="speE"
                     /locus_tag="OO172_000746"
     CDS             13154..14020
                     /gene="speE"
                     /locus_tag="OO172_000746"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5511030.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14036..14830
                     /gene="speD"
                     /locus_tag="OO172_000747"
     CDS             14036..14830
                     /gene="speD"
                     /locus_tag="OO172_000747"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5511031.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(14868..15230)
                     /locus_tag="OO172_000748"
     CDS             complement(14868..15230)
                     /locus_tag="OO172_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5511032.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15406..18003)
                     /gene="acnB"
                     /locus_tag="OO172_000749"
     CDS             complement(15406..18003)
                     /gene="acnB"
                     /locus_tag="OO172_000749"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5511033.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18358..20211
                     /locus_tag="OO172_000750"
     CDS             18358..20211
                     /locus_tag="OO172_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR5511034.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNSITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQATASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20282..21712)
                     /gene="lpdA"
                     /locus_tag="OO172_000751"
     CDS             complement(20282..21712)
                     /gene="lpdA"
                     /locus_tag="OO172_000751"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5511035.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPKAKKK"
     gene            complement(22037..23929)
                     /gene="aceF"
                     /locus_tag="OO172_000752"
     CDS             complement(22037..23929)
                     /gene="aceF"
                     /locus_tag="OO172_000752"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5511036.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPVAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(23944..26607)
                     /gene="aceE"
                     /locus_tag="OO172_000753"
     CDS             complement(23944..26607)
                     /gene="aceE"
                     /locus_tag="OO172_000753"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5511037.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26768..27532)
                     /gene="pdhR"
                     /locus_tag="OO172_000754"
     CDS             complement(26768..27532)
                     /gene="pdhR"
                     /locus_tag="OO172_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5511038.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28073..29446
                     /gene="aroP"
                     /locus_tag="OO172_000755"
     CDS             28073..29446
                     /gene="aroP"
                     /locus_tag="OO172_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5511039.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29489..30343)
                     /gene="ampE"
                     /locus_tag="OO172_000756"
     CDS             complement(29489..30343)
                     /gene="ampE"
                     /locus_tag="OO172_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5511040.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30340..30891)
                     /gene="ampD"
                     /locus_tag="OO172_000757"
     CDS             complement(30340..30891)
                     /gene="ampD"
                     /locus_tag="OO172_000757"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5511041.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            30979..31872
                     /gene="nadC"
                     /locus_tag="OO172_000758"
     CDS             30979..31872
                     /gene="nadC"
                     /locus_tag="OO172_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5511042.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNDKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32075..32515
                     /gene="ppdD"
                     /locus_tag="OO172_000759"
     CDS             32075..32515
                     /gene="ppdD"
                     /locus_tag="OO172_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5511043.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32525..33910
                     /gene="gspE"
                     /locus_tag="OO172_000760"
     CDS             32525..33910
                     /gene="gspE"
                     /locus_tag="OO172_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCR5511044.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAVQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            33900..35102
                     /gene="hofC"
                     /locus_tag="OO172_000761"
     CDS             33900..35102
                     /gene="hofC"
                     /locus_tag="OO172_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5511045.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OO172_000762"
     CDS             complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="OO172_000762"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5511046.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36342..36380)
                     /locus_tag="OO172_000763"
     CDS             complement(36342..36380)
                     /locus_tag="OO172_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511047.1"
                     /translation="MIIKTLKMSARK"
     gene            36405..37025
                     /gene="coaE"
                     /locus_tag="OO172_000764"
     CDS             36405..37025
                     /gene="coaE"
                     /locus_tag="OO172_000764"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5511048.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLIENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37025..37768
                     /gene="zapD"
                     /locus_tag="OO172_000765"
     CDS             37025..37768
                     /gene="zapD"
                     /locus_tag="OO172_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5511049.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37778..37975
                     /gene="yacG"
                     /locus_tag="OO172_000766"
     CDS             37778..37975
                     /gene="yacG"
                     /locus_tag="OO172_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5511050.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OO172_000767"
     CDS             complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="OO172_000767"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5511051.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OO172_000768"
     CDS             complement(38524..41229)
                     /gene="secA"
                     /locus_tag="OO172_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5511052.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKLGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OO172_000769"
     CDS             complement(41291..41803)
                     /gene="secM"
                     /locus_tag="OO172_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5511053.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OO172_000770"
     CDS             complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="OO172_000770"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5511054.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OO172_000771"
     CDS             complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="OO172_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5511055.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OO172_000772"
     CDS             complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="OO172_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5511056.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OO172_000773"
     CDS             complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="OO172_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5511057.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OO172_000774"
     CDS             complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="OO172_000774"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5511058.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OO172_000775"
     CDS             complement(47268..48743)
                     /gene="murC"
                     /locus_tag="OO172_000775"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5511059.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OO172_000776"
     CDS             complement(48797..49864)
                     /gene="murG"
                     /locus_tag="OO172_000776"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5511060.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNGWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OO172_000777"
     CDS             complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="OO172_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5511061.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51105..52421)
                     /gene="murD"
                     /locus_tag="OO172_000778"
     CDS             complement(51105..52421)
                     /gene="murD"
                     /locus_tag="OO172_000778"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5511062.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="OO172_000779"
     CDS             complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="OO172_000779"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5511063.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53500..54858)
                     /gene="murF"
                     /locus_tag="OO172_000780"
     CDS             complement(53500..54858)
                     /gene="murF"
                     /locus_tag="OO172_000780"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5511064.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIARTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEDCHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54855..56342)
                     /gene="murE"
                     /locus_tag="OO172_000781"
     CDS             complement(54855..56342)
                     /gene="murE"
                     /locus_tag="OO172_000781"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5511065.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="OO172_000782"
     CDS             complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="OO172_000782"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5511066.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="OO172_000783"
     CDS             complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="OO172_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5511067.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="OO172_000784"
     CDS             complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="OO172_000784"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5511068.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="OO172_000785"
     CDS             complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="OO172_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5511069.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14443 a        16744 c        14862 g        14364 t
ORIGIN      
        1 ataccaatag aagctggtga gtttaaagcc accagtactt tccaggtaac ctacccttaa
       61 taagtccacc gcaccgccat catctggcgg tgcggtaatt gataaatcat tgcccccacc
      121 aatgtgactg ataaaatgcc aggcaacacg acatcattta tcaggatacg ttatgaaacc
      181 gaccaccatt gcttcacttc agaaatataa gcaggataaa aagcgtttcg cgaccatcac
      241 cgcttacgac tacagcttcg cgaaactatt tgctgatgaa gggcttaacg tcatgctggt
      301 aggcgactcg ctgggcatga ccgttcaggg ccacgattcc actctgccag taaccgttgc
      361 tgatatcgcc taccacactg ccgccgtacg tcgcggcgca ccaaactgcc tgctattggc
      421 tgacctgccg tttatggcgt atgccacgcc ggaacaagcc tttgaaaacg ccgcaacggt
      481 tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt gagtggctgg tcgaaaccgt
      541 aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac ttaggtttaa ccccacagtc
      601 agtgaatatt ttcggtggct acaaagtaca ggggcgcggc aatgaagcga gcgatcgact
      661 actcagcgat gcattagcct tagaagccgc gggggcacag ctgctggtgc tggaatgcgt
      721 gccggttgag ctggcaaaac gtattaccga agcactggcg atcccggtta ttggtattgg
      781 tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac gacgcctttg gtattaccgg
      841 cggtcacatt cctaaatttg ctaaaaattt cctcgccgaa acgggcgaca tccgcgcggc
      901 tgtgcggcag tatatggctg aagtggagtc cggcgtttat ccgggcgaag aacacagttt
      961 ccattaagga gtcacgttgt gttaattatc gaaaccctgc cgctgctgcg tcagcaaatt
     1021 cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc ctactatggg taatctgcac
     1081 aatggccata tgaagctggt cgacgaagcc aaagcccgcg ccgatgtggt cgtcgtcagt
     1141 attttcgtta acccgatgca gttcgaccgc ccggaagatc tggcacgtta tccacggacc
     1201 ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg atttagtttt cgccccttcg
     1261 gtaaaagaga tctacccgaa cggtactgaa acccacactt acgttgacgt tcctggcctt
     1321 tcaaccatgc tggaaggtgc cagccgtccg ggacattttc gcggcgtttc gactattgtc
     1381 agcacgctgt tcaacctggt ccagccggac atcgcctgct tcggtgaaaa agattttcag
     1441 caactggcgt tgatccgcaa aatggttgcc gatatgggct tcgatattga gattgtcggt
     1501 gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt cccgtaacgg ttatctgacg
     1561 gcggaacaac gcaaaattgc gcctggtctg tacaaagttt taagttcgat tgctgacaaa
     1621 ttgcaggctg gggaacggga tctcgatgaa attattacca ttgcggggca agaactgaat
     1681 gaaaaaggct tccgcgccga tgatattcag attcgcgatg ccgacacatt gctggaagtt
     1741 tctgaaacca gcaaacgggc agtaattctg gtagccgcct ggcttggcga tgctcgcctg
     1801 atcgacaaca aaatggtcga gctggcgtaa tacttaactg gcgctacggc tgatggcgcc
     1861 agttattaat ttaccccacg tcatctgtca taacacaggt aacttaaata aggttaatta
     1921 ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc aacgtcttcc cgagacattc
     1981 ctttacttag aagacgcagg gcgaattctt gcctttcctc ctgtcttccc tgcggaatcc
     2041 ccttctcaat ccctttctct tcaaaccact gcgccagcgt catcatagac ttccctcccg
     2101 tttccctgtc tctcagcaca ccataaaaca aatccgcttg ttcagtatga ccgcgttgca
     2161 gcatatagtt ttgcatggca actaactgac ttccgctagt gtacccttcg tcaatcagcg
     2221 tgaccagttg ctccagcaat aacattaagt cgcgctggcg aatatgtttt tgcagtagtt
     2281 cgagaatcgc aatccgccga tgttgcatga tttcgtcatc cggtgtgatg gtgatatcca
     2341 ccagcgggaa aggactgtta tagacgcgtc gcgccagctc cggcgagtaa aacatatcaa
     2401 accagcacat tgagagcgga taaggtgtgg cctcgccctg ataaaacagt atcggcacca
     2461 ccagcggcag cttatcgtgg ccagcctcca gatgccggtg catggcagca atagaataac
     2521 gcatcatgcg aaaggccatt ttcttatccg gcttgctttg gtgttcaatc acaacatgca
     2581 gataaccggg attgccctgc atttgcacgg aatagagcac gtccgtgctg tgacctttca
     2641 ggctctcttc aatgaaactc cccgactcta aatgaagcgt gttgagatca caaagttcgc
     2701 gtaattccac tggcaaatgt atctccagaa agtcgcgagc cgtctccgca tgcattaaaa
     2761 attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc atccatcttc cctgtcttcc
     2821 gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc tatcgtcagc cacctcttta
     2881 ttgtttaccg agcagcattc ataaaaatat caattcgtta caggcgttac atggcacgct
     2941 tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga tgagtagaca ctcatcaaaa
     3001 atcgggcaat actgcgtgag aatttcctca aagcaggcac cgcctgcttc gttaacgaca
     3061 gggtagaaag gtagaagtta tgattcgcac gatgctgcag ggcaaactcc accgcgtgaa
     3121 agtgactcat gcggacctgc actatgaagg ttcttgcgcc attgaccagg attttcttga
     3181 cgcagccggt attctcgaaa acgaagccat cgatatctgg aatgtcacca acggcaagcg
     3241 tttctccact tatgccatcg cggcagaacg cggttcgaga attatttctg ttaacggtgc
     3301 ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc gccagcttcg ttaccatgcc
     3361 agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat tttgaaggcg acaatgaaat
     3421 gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga ttgttatccc tgcggctggt
     3481 tactcaccag ccgcgacatc ttctccagcg aatccgttct taagatataa agtcgtttta
     3541 gtaacaacgg attatccccc ggttttactt tgcctttcat ggttgtcacc gccaggtgaa
     3601 atccggcatc gtttgctgcc ttcacggctt tgtcattaaa gccgccaaac ggatacgaaa
     3661 gataccagac atgtggatta aattgcgcca gagcgcggcg tgaacgtgca aaatcaaaca
     3721 gaatattgtg ctcactacgg ctcagtaata tgggtcggcg ataaccatct acccgatgca
     3781 aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat ttcgttaagc tcagaaacgc
     3841 tcataaattg cagcgatttt gggttccact tctgcgggtg acgtttgatg cgtgaggtaa
     3901 caataaacac cgtcgccttc atgccatatt gtttcaacac aggatacgca tagcggctca
     3961 ccgacttgag gccatcatca aaggtaatca ccaccgctcg cgcagggaga ttgatcgtat
     4021 tcttcacgta accttccagc tgagccatgc tcagtgtcgc gtatcccctg tcgcgcagcc
     4081 aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt cgaagtatgg cgaaaacggg
     4141 tgttttcttc gtcgcgcaga atatggtgat aagttagcac cggcaggcca ttatcgggtt
     4201 gggcatccag cgcgctgata taggccagtc gatcgccaat acggatctga taccaggttt
     4261 gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg caaattgtcc gccagtaccc
     4321 caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg cgtatctttc caggtaatta
     4381 agttctgatt actcagcggc ttgttaaggt cacccaaacc gtcttcaact ttttgtcgcc
     4441 cctgaactgg ctcgagatgc cctttatcga taaacccttt accaaagcca aaattaaatg
     4501 cgtaataact tgcggcagtg ggctctacgg caatgatttg tccggcccga atattcccca
     4561 cggtcaccat cttgtcacca atttgcgccc agaccgcagc attttcgatg gtttgcatat
     4621 aacgggcagg taacgcggca ctgacacttg cggtaaacag catcagcagg agaataacag
     4681 cttgtttata catgaaaaaa taaccgaggg tgaaacaacg cttttattgt agcaaaagcg
     4741 ccatcagaat cgcgggttaa tactcataca aattatgttt gaggacgaaa ggcggatttt
     4801 tttgttgttg gtgccaaaga ctactcacct ccggcccacc cagttcgaca atacactcac
     4861 gaaactctga actggtcatg gtttcacggc aagccatcat ctgttcaatt aacggtaacg
     4921 tgacaccaga aatcacttcg caacgggagt gtttgtggct taataatgaa gccacgcgat
     4981 acggtggcgc tccggctata tcggttaaga agatgacacc ctcaccagag tccgcttcat
     5041 gcagagcatc gcacatcatg cggctgagca tattagagct taatccgcgc cagaaattca
     5101 cggcccggca ctgaagaagt gccccatgtt ttttctccag cgcatccagt atctcttgcg
     5161 cccggtcatc gtgacaggta attacccaac ctaacatcgc cttcctcctt agccgaaaat
     5221 cagcctaccc tgaccattgt tgataaaggg tgatagccgt caaaactcct ctcccccgaa
     5281 tccagaggag aggaaatagc cttagctacg caaaccacgt ccacgttgga tcagcgacca
     5341 acagatcaaa taaaacgcca caataaagac caccagtacg ccaaaggtag tgaccagcgg
     5401 cacatcattt ataccgagga agccgtagcg gaaaccactg atcatataaa cgattgggtt
     5461 caggtgcgac agcccttgcc agaacggcgg caacaaagtc agtgagtaaa agaccccgcc
     5521 caaatacgtg agtggcgtta acacaaaggt tggcaccagg ctgatgtcat cgaacgtttt
     5581 ggcaaacacg ccgttcagca aacccgcaag ggagaatagc accgccgtga gcaccagggt
     5641 taaggcaacg aatacccacg aatgcacctg aaacggcaca aaaaacagtg aaattgccgt
     5701 caccagaatg ccaacaaaca gaccacgcgc cacgccaccg ccgacatatc cggcaataat
     5761 gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata ttacgctgga acttggcacc
     5821 aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc accgacatca tgatcagccc
     5881 cggtacgatg aactgcatat agctgaagcc atgcatatcg ccaatacgcg aacctatcag
     5941 gttaccgaag ataataaagt aaagggtcat ggtgatgact ggcggcacca gcgtctgcac
     6001 ccagatacgc ataaagcgat ggatctcttt cgcccagatg ctttttagcg ccacccagta
     6061 aagatgcatc atgcgcgatc tccttgtttt tcattaacca gtgaaacaaa cagctcttcc
     6121 agacggttag ctttgttacg catacttaat acctgaatgc cctgctcact taactgcgta
     6181 aatacgctgt tgatcccctg ctcacgtagc acttcaactt ccagcgtcgc agtatcgacc
     6241 agtcgatact gatagccatc gagcttcggt aacggacttt tcggtgcgag atcgagaata
     6301 aaggtttccg atttcagctt cgccagcagc gccttcatcg aggtattttc caccagctca
     6361 ccatgttgaa taatgccgat attgagacac agcatttctg cttcttccag gtagtgtgtg
     6421 gtaagaatga tggtggtgcc tttgtcgttt aaatccttca aaaagcccca cattgagcgg
     6481 cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa tcagtagttt aggttcatgc
     6541 attaacgcac gggcaatcat taaacggcgc ttcatcccgc cagataacat acgcgcacgt
     6601 tcgttgcgtt ttccccataa atcgagctgc ttaagatatt tttcgctgcg gatgtacgcc
     6661 tctttgcgct ccacaccgta gtaccctgcc tgattcacca caatttgctg cacggtttca
     6721 aacgggttga agttaaattc ctgcggcacc agtcccaact gacgtttagc gttcacgaca
     6781 tctttctcga gatcgtaacc aaatacgctg acccgcccgg aggttttatt taccagagag
     6841 ctgataatac cgatagtggt cgatttcccg gccccgttcg gcccgagaag cgcataaaaa
     6901 tcacccgctt cgacctgcaa atctattcca cgaagcgcct gaacgccgcc tggatagttt
     6961 tttttaagct gttgaagttc cagtgcaatg gtcataaatt tttacttacc ttacgttctt
     7021 acactttatc tgtggtttaa atcgtcccgg agttgcccta tattagccaa acgtaattat
     7081 ttggttacag gtcgttaacc tccatgaaag acatagatac actcatcagc aacaatgcac
     7141 tatggtcaaa aatgctggtg gaagaggatc ccgggttttt tgagaaactg gcacaagcgc
     7201 aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg cgttcctgca gaacgtttaa
     7261 ccggtcttga gccgggcgaa ctctttgttc accgtaatgt tgctaacctg gtcattcaca
     7321 ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga tgtactcgaa gttgaacaca
     7381 ttattatctg tggccactac ggttgcggcg gcgtacaagc cgccgttgaa aacccggaac
     7441 tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa catagctcat
     7501 tgctcggcga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg aacgtcatgg
     7561 aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa cgcgggcaga
     7621 aagttaccat tcacggctgg gcctacggca ttcacgacgg cttgctgcgt gatctggatg
     7681 ttaccgccac caaccgcgaa acccttgagc aacgttaccg tcacgggatt tccaacctca
     7741 agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca tccggcaact
     7801 tcacacttac tcgtccagca gaatcacttt gccaatatac ggcagatgac ggtaacgctg
     7861 tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac cgataaattc
     7921 aaccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa tcgccaacga
     7981 cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc ccgagtcgat
     8041 gatatcttca acaatcagca cgtccttgcc acggatatct tcatccagat ctttgaggat
     8101 tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca taaagtcgac
     8161 ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg agccacgcag
     8221 cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa tctgacgacc
     8281 cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta cagtatgttt
     8341 catatctcta accatatgat ttaaaaacaa atcacttcgc acgggcaggt tttggcaccg
     8401 caacaaagcg caagacacgc agtataccag taaaactatt cattcgcgga atctgttaat
     8461 aaagctcgaa ttgtgatgac ggtcacacat gttaaaatct ataattaatt gctactaaaa
     8521 tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacaggct cgcgacgatt
     8581 actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac tcattggcgg
     8641 aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc ttgtgatgct
     8701 cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg ctttggctat acgcagccct
     8761 gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct gggcgctgac
     8821 tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt ttgtctggcg
     8881 tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtcgcac tgctgattag
     8941 cggtggtatc ctgacctggg ccggatttaa cgatccgcag gagatcaacg gcaccttaag
     9001 cgccgatgcc acacctgccg aagctatctc ccccgttgcc gatcaggact ggcctgcgta
     9061 tggccgcaat caggaaggtc aacgcttttc accactgaag caaattcacg ccgataacgt
     9121 ccacaagctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc cgaacgatcc
     9181 gggtgaaatc accaatgaag tgacgccgat taaagtgggc gatacccttt acctgtgtac
     9241 cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc aaagagaaat ggcattacga
     9301 tcctgagctg aaaaccaacg agtctttcca gcacgtaacc tgccgtggcg tctcttatca
     9361 tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc gtcgtatcat
     9421 tcttccagtg aatgatggtc gcctgattgc gattaacgct gaaaacggca aactgtgcga
     9481 aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca ccaaaccggg
     9541 tctgtatgag ccgacttctc cgccaattat caccgataaa accatcgtga tggccggttc
     9601 agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt ttgatgtcaa
     9661 caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg caatcccgtc
     9721 tgacgaacac gcctttacct ttaactcacc aaactcgtgg gcaccagcgg cctatgacgc
     9781 gaagctggat ctggtctatc tgccgatggg cgtgaccacg ccggatatct ggggcggtaa
     9841 ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg ccactaccgg
     9901 gaaactggcg tggagctacc agaccgttca ccacgacctg tgggacatgg atcttccggc
     9961 acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccgatta tttacgctcc
    10021 ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat ggcgaactgg tggttccggc
    10081 cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc ctactcaacc
    10141 attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata tgtggggagc
    10201 caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct atgaaggcat
    10261 tttcacgccg ccatctgaac agggtacgct ggtcttcccg ggtaacctgg ggatgttcga
    10321 atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc caatggcact
    10381 gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac ccgatggagc agccgaaaga
    10441 tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac cgtatggtgt
    10501 cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag catggggtta
    10561 tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta ttggtacgcc
    10621 gcaggacagt atgccgttcc cgatgccggt tccggtgccg ttcaatatgg gtatgccgat
    10681 gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcaccgcta cggcagataa
    10741 ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc gtttaccggc
    10801 gggtggtcag gcaacaccga tgacctatga agtgaatggc aagcagtacg tggtgatctc
    10861 cgcaggcggt cacggttcat ttggtacgaa gatgggcgac tatattgtgg cttatgccct
    10921 gccggatgat gtgaaataac cataatgccc ggagaaatcc gggcatattt ccgaatacgg
    10981 tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag cagatggcag
    11041 tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa acttcaccag cacttcactg
    11101 acattgcctt ctaccttaac ggtatctttc cagcccgcgc gatgagccgc tggcggtttg
    11161 ccattttctg acaagatacg gaactgcgtg ccgtgaatat ggaacggatg cagcatcatg
    11221 tcgcccacgc cagagataac ccaacgttcg tactgccctt tcgccgccgc aaacatcggc
    11281 ttattcatat caaacgcctg accgttgatt ttattggcat ggtggaaatc gaacttcccg
    11341 ccgtggttca tatgattcat attgccgtgc cccatatggc ccatcatctg gctgtgatcc
    11401 attccggcca tcgcctgatc gccatatttc tccattagca tctgcatccc catcatatcg
    11461 agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag cgaaggtaac
    11521 gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg cactaatagc aatcggctga
    11581 atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg ccatccccat ctggctgacc
    11641 ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac ttcaaaacgc
    11701 tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt caggtagcag accaccgtcg
    11761 ctggcaatca catacagcgg gcgattgtcg ctggtggcga aattgagcga gcgggcatta
    11821 cagccattga gcaaacgcag gcgcagccaa ccacgcgggg cagcgtgttg tgggtagatt
    11881 gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg cggcggtcat cacatccagt
    11941 tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct gaacgatcac cggaacatca
    12001 tcgatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc aatcaccacc
    12061 agaccagcca gccccatcgc cacctgtcgc cggtcttgcc atgttgatgt ggatggaacc
    12121 agcaggtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca cctggcggaa
    12181 taattccctg tgggccgccg tcgacgtcac ccggtacttc cagcccgtgc cagtgcaacg
    12241 tcgtctcttc cgccagttgg ttgtagatat caaccgttac cgctttgccg cgctgtaatt
    12301 tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcagtt ttctcgccaa
    12361 aggtggactg acctgcgcca atagttaact gaatgcgatt acgggcatcg gtcgtgagca
    12421 aatcagggat cggtaacgtt gggcgttccg ccgcaaagac tgcacggctc cacagtggca
    12481 aggctgaagc cacacccagt gcgacggaat atttcaagaa atcacgacgt tgcatagtta
    12541 tttccttatt cttaagcagg caaaacaaaa tcaaacgttg agcatagtcc ttccccttac
    12601 gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc tcgctcgcag
    12661 tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt ttttcagaac
    12721 agtgttattc ggcagcctga tcgccgtttg cgcaaacagt tacgcgctca gcgagtctga
    12781 agccgaagat atggccgatt taacggcagt ttttgtcttt ctgaagaacg attgtggtta
    12841 ccagaactta cctaacgggc aaattcgtcg cgcactggtc tttttcgccc agcaaaacca
    12901 gtgggatctc agtaattacg acaccttcga catgaaagct ctcggtgaag acagctaccg
    12961 cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc aaagccctgg cccgcgattc
    13021 cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa atattgaccg
    13081 tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt ttttacgggt gttaacaaag
    13141 gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa acgctacacg accagtttgg
    13201 gcagtacttt gcggtagata acgttctgta tcatgaaaag accgatcacc aggatctgat
    13261 catttttgag aacgctgcat ttggtcgcgt aatggcgctg gatggcgtag tacaaaccac
    13321 cgagcgcgac gagtttatct atcatgagat gatgacccat gttccgctac tggcccacgg
    13381 tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt gccatgctgc gtgaagtaac
    13441 ccgacataaa aacgttgagt caatcacgat ggtggaaatc gatgcgggtg tcgtgtcgtt
    13501 ctgccgtcag tatctgccca accataacgc cggtagctac gacgatccgc gctttcaact
    13561 agtgatcgac gatggcgtca atttcgttaa tcaaaccagc cagacctttg atgtcattat
    13621 ctccgactgc accgatccta tcggtcccgg cgaaagcctg ttcacctcag cattttatga
    13681 aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg gcgtctgctt
    13741 tttacagcag gaagaagcca tcgacagcca tcgcaaactc agccattact tcagcgacgt
    13801 tggcttttat caggcggcga tcccgaccta ttacggcggt atcatgactt ttgcatgggc
    13861 gacagataac gacgccttac gccatctctc aaccgaaatt attcaggcgc gttttctcgc
    13921 ttctggcctg aaatgccgtt attacaatcc ggcaatccat acggcagctt ttgccttacc
    13981 tcagtatctg caagacgcac tggcttcaca gccgtcctaa ggagaagata agaaattgaa
    14041 aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt gtatttacga
    14101 tatctgctac gccaaaactg ccgaagagcg cgacggttat attgcttata tcgatgaact
    14161 ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta tcggggccaa
    14221 tattcttaac atcgcccgcc aggattacga accacagggt gccagcgtca ctattctggt
    14281 gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca gaacaccccg gcccactgcc
    14341 agaaacggtg gttgctcatc tcgataaaag tcatatttgc gtacatacct acccggaaag
    14401 tcatcctgaa ggcggtttat gtaccttccg cgccgatatt gaagtctcta cctgcggcgt
    14461 gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg atatcgtaac
    14521 cattgattat cgcgtgcgcg gttttactcg cgacattaac ggtatgaagc actttatcga
    14581 ccatgagatt aattcgattc agaactttat gtctgacgat atgaaggcgc tgtatgacat
    14641 ggtggatgta aacgtctatc aggaaaatat cttccatacc aagatgttgc ttaaagagtt
    14701 cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca gcgagcgcca
    14761 ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc gcaatatgcc
    14821 agctgtttaa cggctctggc ggagctccca ggctccgcca gatttattta cttctgctgc
    14881 acgaaattgc ggtaagccgc cacgacctgc agaaaatcct caacgccgca tagcgacagg
    14941 ctttcttcgt cgtagtagtt catcccctct tccatttcat cgccagcgaa ttccagttga
    15001 ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg tgtattcatg ccctgcccgt
    15061 tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt gttccacttc atcaagcaag
    15121 gccaggtttt ctttcacctc ttcattaaac cagtgcccga ccacttcatg ccccatggac
    15181 atacgcacct ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat agtgtgttcc
    15241 tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg atgtaattat cgcagcctta
    15301 ccggagaaaa acagcggaac ggcgatggtt tagaaataaa aaatgcccgg gaatctcatt
    15361 caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt acttattaca ccgcagtctg
    15421 gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc tggttgaagt tcagataacg
    15481 ataagtatca acggcagttt tatctacttg cgcaacgtag gtctggtact cttccggcgt
    15541 cggcagtttg ccaatcagcg ccgcaacagc cgccagttct gcagaagcca ggaagacatt
    15601 cgcgccagta cccaaacggt tcgggaagtt acgggtagag gtggaaacca ccgtcgcacc
    15661 gtctgctaca cgcgcctggt tacccataca cagggaacag cccgggatct cgatacgagc
    15721 cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc agttgcgcgg cgtccatacg
    15781 ggttggcggt gccacccaca ggcgggtcgg caactgacct ttgtgcgcat ccagcagttt
    15841 accagcggca cggaagtgac cgatgttagt catgcaagaa ccgataaata cttcgtcgat
    15901 cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc ggatcgttcg gagcacacag
    15961 gattggctct ttaatctccg ccagatcgat gtcgatcact gccgcgtatt ccgcatctgc
    16021 atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa tacggcgttc
    16081 caaggtacga cgatcgccgt aaccttccgc gatcatccac ttcagcagga cgatgttgga
    16141 gctcaggtat tcgatgatcg gttctttgtt cagcttgata gtacaaccag cggcagaacg
    16201 ctcggcggac gcatcggtta gctcaaaggc ctgctcaact ttcagatccg gcagaccttc
    16261 aatttccaga atgcgaccag agaagatgtt tttcttgcct ttcttctcaa cggtcagcag
    16321 accttgtttg atcgcataca gcggaatggc gtgtaccaga tcgcgcaggg tgatgcccgg
    16381 ctgcattttg cctttgaagc gcaccagaac ggattccggc atatccagcg gcattacgcc
    16441 agttgcggca gcaaacgcca ccagaccaga acccgccggg aaagagatac cgatcgggaa
    16501 acgggtatgg gagtcaccac cggtaccgac ggtatccggc agcagcatac ggttcagcca
    16561 ggagtgaatg acgccgtcac ccggacgcag cgacacaccg ccacggttca taatgaagtc
    16621 cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg tgtggcagaa
    16681 agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca gttcatcacg
    16741 ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc attttcggtt cgcagtacgc
    16801 gcccggacga atgcctttca cgccacaggc acggcctacc attttctgcg ccagcgagaa
    16861 gccgcgatcg ctctcagcga catctttcgc ctgacggaac acatcactgt gcggcagacc
    16921 aagtgcttca cgagctttgg tggtcaggcc acgcccgata atcagcggaa tacggccgcc
    16981 agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca gcagttcgcc
    17041 tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaatcacgt cgcccatgtt
    17101 caggttagag acgttgactt cgattggcag tgcacccgcg tcttccatcg tgttaaagaa
    17161 gatgggtgca attttaccgc cgaggcacaa accaccgccg cgtttgttcg gcacatgtgg
    17221 aatatcatcg cccataaacc acagaacgga gttcgtggcg gatttacgcg atgaacccgt
    17281 acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag cttcaatctg
    17341 tttgatcggg ccaacaacac ctggctggtc tggctcaata ccttcacggg cgtttttcag
    17401 catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac gcatccggtg ccggagaaag
    17461 gtcatcggtg ttggtttcgc cagtgacttt aaagacggta acggtcagtt tttcagccag
    17521 cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcattacct gcttcgcata
    17581 ttcgttgcct gctttcgctt tctcttctac gtcatagaag ttatcgaaca tcagcagcgt
    17641 gtgagaaagt gctttggcag caataggtgc cagtttggca tcatccagcg cgtcgatcag
    17701 cggatgaatg ttgtaaccac cctgcatggt gcccagcagt tcgatagctt tttccggagt
    17761 cagcagaggg gatttagctt cgcctttagc gatagcagcc aggaagcctg cttttacata
    17821 ggcggcttca tcgacgcctg ggggaacacg gttggttaac agatctaaca ggaattcttc
    17881 ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg gccatttggt ttgcatccag
    17941 gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt attcttctag
    18001 cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct tcacgctcct
    18061 gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat agcaggattt
    18121 tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga atcgttaagg
    18181 atggtctaga gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt gataatcacc
    18241 tttctcaacg aatcccaaga ttagtcaaaa tttaaactac cgcctcttta tactcggatt
    18301 cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat ccgaattatg
    18361 aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc cgggctttgt
    18421 gctgcctctg ccgggctata tataaaaagc cgcacagtgg aagcgcctgt agaaccgcaa
    18481 tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc ttcctgcaac ggtttccgca
    18541 cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca ctgcacaaat agatcaatgg
    18601 gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc aggtgctgat ggcatcaacc
    18661 tatccgacaa acgttgctca agcagtgcaa tggtcgcacg ataatccact taaacaaggc
    18721 gatgctgcta ttcaggcggt atctgaccag ccgtgggacg ccagcgttaa atcactggtg
    18781 gcctttccac aattgatggc attgatgggc gaaaacccgc aatgggtgca aaacctgggc
    18841 gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt gcggcaactg
    18901 gcgcaacaaa ccggctcgct gaagtcatca accgaacaga aagttattac cacaacgaag
    18961 aaagctgtac cggtaaaaca gacagtcacg gcacccgtca taccatccaa taccgtttta
    19021 actgccaacc ccgtcattac agagccggca acaaccgtca tttccattga gcccgccaat
    19081 cctgatgtgg tctatattcc caactacaac ccaaccgtgg tttacgggaa ctgggccaat
    19141 actgcgtatc cgccggttta tctgccacca ccagccggag aaccgtttgt tgacagcttt
    19201 gtgcgcggat tcggctatag catgggcgtt gctaccacgt acgcactatt cagcagcatc
    19261 gactgggatg acgacgatca tgaccatcat catcatgacg atgatgatta tcatcaccac
    19321 gatggcggtc atcgtgacag taatggctgg caacacaacg gcgacaacat caatatcgac
    19381 gtcaacaatt tcaacagtat caccggtgag catcttactg ataagaatat ggcatggcgg
    19441 cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc aaagcggttt
    19501 catcaaaccg atgtcaacgg cggaatgagt gccacgcagc tacctgctcc aacacgcgac
    19561 agccagcgtc aggcgacagc aagtcagttt cagcaacgaa cacacgccgc ccccgtcatt
    19621 acacgagata cccaacgtca ggcagcggca cagcggttta atgaagctga acactatggg
    19681 agctatgacg acttccgcga cttcagccgt cgccaaccac tgacccagca gcaaaaggac
    19741 gccgctcgtc agcgttatca gtcggcctct cctgagcagc gccaggcagt ccgcgagaaa
    19801 atgcagacta acccgcagat ccagcagcga agggaggcag cgcgccagcg tattcagtcc
    19861 gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc agactaaccc gcagaaccag
    19921 cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa ctcccgaaca gcgccaggtg
    19981 tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa cgcccgccag
    20041 cgtatacaat cggcatcacc tgaacaacgt caggtttttc gggagaaagt tcaggagagc
    20101 cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga gcaacggtca
    20161 acagtacgcg aacgtctctc tgaacgtgga gcaagaagac tggaaaggta aattgcagac
    20221 gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg caaacgaaaa
    20281 attacttctt cttcgctttc gggttcgggt tcggcaggtc ggtaatgcta ccttcgaaca
    20341 cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga
    20401 tgtcttcagc atcacaaccc atttcgattg ccagcccgat ttcacccagc agctcgccgc
    20461 cgttggtacc aacaatcgca ccaccgatca cgcggtgaga ttctttgtcg aaaatcagct
    20521 tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc cacgggaagg
    20581 tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga cctacccatg
    20641 caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag tagtgtttct
    20701 taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg
    20761 gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc agctgtttgt
    20821 caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg tcgaggtttt
    20881 taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc ggttcagctg
    20941 gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca acggcggtaa
    21001 ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag actttaacga
    21061 tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca atctgtgaac
    21121 ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc attaccagca
    21181 ggcgttctgg cacttctttc agttccagcg cgtcagtgga gtcccagata cgcggatctt
    21241 catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc gcattgtcga
    21301 agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc ccggtgaatt
    21361 tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc agaccaccgg
    21421 tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg gttttcggtt
    21481 cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact tttgctacgt
    21541 gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca agggtgttgt
    21601 aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca gcggagtaac
    21661 ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc atcatgacct
    21721 ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg gcgttagctt
    21781 cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc gcttgctacc
    21841 ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc accgggtcgt
    21901 tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac ggcaaccgat
    21961 ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa gccggccgtt
    22021 gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg ttgttaatga
    22081 tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa
    22141 tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc atcgcggact
    22201 tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag tgggtagtac
    22261 ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc gcagtcagct
    22321 taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg atgatgcctt
    22381 tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc accgccacac
    22441 cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc gaactattga
    22501 agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca accggggtga
    22561 tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca
    22621 gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc cagttacggc
    22681 tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg atttcgccaa
    22741 acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg ccagtcgccg
    22801 ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg tcttcgcgca
    22861 ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca aactcgcgtg
    22921 ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca aattcagatt
    22981 tgccttccgc tttcgcagcc ggagctgctg ccggagcttc agcttttgct gccggagccg
    23041 gcgctgccgc ttcctgtttc gcaggagctg ccgcaggtgc tgcgccttca acttcgaaga
    23101 tcataatcag cgaaccagtt ttcactttat cgccaacgtt gactttcagt tccttcacga
    23161 cgcctgcaaa cggagccgga acttccatag aagctttatc gccttctacg gtaatcagtg
    23221 actgttcagc ggcaactttg tcgcccactt tcaccatcac ttcggtcact tcaacttcgt
    23281 caccgccgat atccggaacg ttaacttctt tcacgccagc tgctggtgcg ggggtcgctg
    23341 ccggagtcgc ttcctgttta gccgccggag ctgccgcgcc tgcttcaccc gcgacttcga
    23401 agaccataat cagcgagccg gtagacactt tgtcacccac gttcactttg atctctttca
    23461 cggtacctgc aaacggagcc ggaacttcca tagaagcctt gtcgccttct acggtgatca
    23521 gcgactgttc agcttcaact ttatcgccaa ctttcaccag gatttcggtc acttcaactt
    23581 cgtcgctgcc gatatccgga acgttaacgt cttttgccgc cgcagccgcg ggtgctgcta
    23641 ccggagctgc ttctttcttc tcttctgcct gagcaggtgc agcgtctgct gcaccgtcgg
    23701 cggaatcgaa aatcataatc agtgcgccgg tctgggtttt atcgccaaca gagactttga
    23761 tctctttaac gatacccgcc tgcggagatg gaacttccat agaggctttg tcgccttcta
    23821 cggtgatcag cgactgttcg gcttcaactt tgtcgcccac tttgaccagg atctcggtga
    23881 tttcaacttc atcagccccg atgtccggta ctttgatttc gatagccatt attcttttac
    23941 ctcttacgcc agacgcgggt taactttatc tgcatcgatg ttgaatttgg cgattgcgtc
    24001 agcaaccact ttcttatcga tttcgccacg tttagccagt tcgcccagcg ccgcaaccac
    24061 gacataagaa gcatcaactt cgaagtggtg acgcaggttc tcacggctgt cggaacgacc
    24121 gaagccatca gtacccagta cgcgatagtc gtcagccggt acgtaagtac ggacctgctc
    24181 agcgaacagt ttcatatagt cggtagatgc cactgccggc gcgtcgttca tcacctgagc
    24241 gatatacgga acgcgcggag tttccagcgg atgcagcatg ttccagcgtt cacaatcctg
    24301 accatcacgc gccagctcgg tgaaggaggt cacgctataa acgtcagaac ctacgccgta
    24361 atctttcgcc aggatctctg ccgcttcacg gacgtgacgc aggatggaac cggagcccag
    24421 cagctgaact ttacctttgc taccttcaat ggtttcgagt ttgtagatac ctttacggat
    24481 accttcctca gcaccttccg gcattgccgg catgtggtag ttttcgttca gcgtagtgat
    24541 gtagtagtaa acgttctctt gtttttcacc gtacatacgc tccagaccgt catgcataat
    24601 gacagcaact tcgtaagcgt aagccgggtc gtaagagata cagttcggga tagtcagcga
    24661 ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttca ccgttcaggg tggtacgacc
    24721 ggaagtaccg ccgatcagga agccacgcgc ttgctggtct ccagccgccc agcacagatc
    24781 gccgatacgc tggaagccga acatcgagta atagatgtag aatgggatca tcggcagatt
    24841 gttcgtgctg taagaggtcg ccgctgccag ccaggaacac cctgcgccca gctcgttgat
    24901 cccttcctgc agaatctgac ctttctcgtc ttctttatag taagcaacct gctcgcggtc
    24961 ctgcggggtg tactgctgac cgttcgggct gtaaatacca atctgacgga acagaccttc
    25021 cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc agacgatctt tgatcgactt
    25081 gttcttcagc atcacgttca gagcacgaac gaaagcgata gtggtagaga gctctttgct
    25141 ctgctcttcc agcagcgcgc cgaagtcttg caggctcggc agctcaagct tctcggtgaa
    25201 gttcggctga cggcttggca gataaccgtg cagtttctga cgctgagcgt gcagataggt
    25261 atgctcttca gaaccttccg ggaaggtgat gtacggcagt ttttcgatat ctgcatcaga
    25321 caccggcaca ttgaaacggt cgcggatgtg acgcacgccg tccatgttca ttttcttaac
    25381 ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg ccgtaacctt taatggtatg
    25441 agcaaggatt actgtcgctt tgcctttggt ttcctgcgct ttcttgaatg cagcgtagat
    25501 tttcttcgga tcgtgaccac cacggttcag tgcccagatc tgctcgtcag tccagtctgc
    25561 aaccagtgct gcggtttcag gatatttacc gaagaagtgt tcacgaacgt acgcaccatc
    25621 tttcgatttg aaggtctggt agtcgccgtc aacggtttcg ttcatcagct ggatcagttt
    25681 accgctggta tctttacgca gcagttcatc ccaacggcta ccccacatca ctttgatcac
    25741 gttccagcca gcaccttcga agatgccttc cagttcgttg atgatcttgc cgttaccggt
    25801 gaccgggccg tcaagacgct gcaggttaca gttgataacg aaaaccaggt tatccagttt
    25861 ttcacgggta gcgatggtga tcgcaccttt ggattccggt tcgtccattt caccgtcgcc
    25921 gaggaacgcg taaacggtct gtttagaggt atctttcagg ccacggtgtt ccagatattt
    25981 caggaattta gcctggtaaa tagcaccaat cggacccaga cccatagata cggtcgggaa
    26041 ctgccagaat tccggcatca gtttcgggtg cggataggaa gagaggccat tgccgtgaac
    26101 ttcctgacgg aagttatcca gctgctcctg agtcagacga ccttccagga aagcacgggc
    26161 gtaaacgccc ggggagatgt ggccctggaa gtaaaccagg tcgccgccat cctgctcgtt
    26221 gcgtgcacgg aagaagtggt taaagcacac atcataaatg gttgcggaag actggaagga
    26281 cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca cgcagcaccg tcatgatggc
    26341 gttccagcgg atagctgaac gaatacggcg ttccagttcc agattacccg gatactccgg
    26401 ttgttcttca acggggatgg tgttgatgta gttgctgata cctgtgcctg cggctacgtt
    26461 tacaccgcct ttgcgggctt cagcaagcag ttggtcgatc agatactgag cacgctcaac
    26521 accttcttca cggatgaccg attcgatcgc ctggagccag tcgcgagttt cgatcggatc
    26581 cacgtcattt gggaaacgtt ctgacatggg ttattcctta tctatctaat aacgttgagt
    26641 tttctggaac ctgtcccatt gaactctcgc cagaaagctc aataagacag gttctacgtt
    26701 tagttaccgc gcttttatat gcgcttgatt tacaacatct tctggataat ttttaccaga
    26761 aaaatcacta attctttcgt tgctccagac gacgcagaga acgctcacgg cggctctctt
    26821 cacggcttct gtcgagcaaa atttcttcga taaaggccag atggcgatgc gatgcttcgc
    26881 gcgcttcttc cggcttaccg gccataatcg cttcaaatat gcgggtgcgg tgactactca
    26941 ccagcggcag catctcgcga cgcgaataga gcaattcgaa gttctggcgg acattctggg
    27001 ccaacatcgg ctccatacac cttagcagat gaagcagaac cacattgtgg gccgcttcgg
    27061 tgacggcaat ctgatactgg agtacggcgt ttgattccgc gtccagatcg ccagactgct
    27121 gcgccagctc tatggcgtgg tggagttcac ggatgcgttc cttgtcttca tcggtactac
    27181 gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg tcgtgtttcg agcaagtcat
    27241 actgtgattc aggatggtcg gagagcagct ccaccagcgg atcgctgaag ctttgccata
    27301 ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag caacaagccc ttcgcttcga
    27361 gacgttgaat cgcctcacgc aaggagggac gggagacgtc gaactgtttt gccagttcgc
    27421 gttccggtgg gagtttttcg cccgggcgga gagtgccttc gaggatcaaa aactccagtt
    27481 gctgctcaat cacatcggag agttttggtt ggcggatttt gctgtaggcc atgagttcct
    27541 gtcttaagcc acttgccgaa gtcaattggt cttaccaatt tcatgtctgt gacgctaaag
    27601 taacaaagta ttcaccttat gtccatacag gttttgattg aaatcatgaa actgtgcaca
    27661 ttttaacaac ttgacatata taacgtttca aagttgtaac tatgcacaaa tgtagacttt
    27721 acgtaggaaa gggatttttt aacgattatt aaactataaa aatccgaatt gaaccgattc
    27781 acttaccaat tttttgattt ttaagtcaat aaaaacgaat ttaaattcac tctgcatatt
    27841 gagaggggtt gaggctgagc tttacaaacg gtttcttttt aagcaactca tcttcaacca
    27901 tgcataaagc gggtgcattc gctgccgcat accattattc ttgatctgac ggaagtcttt
    27961 ttgtaacaat tcaaacttcc ttgatgtaaa taaatcaata caacaaacgg aattgcaaac
    28021 ttacacacgc atcactgcgt agatcaaaaa aacaatcacc gcacgaggtt tcatgatgga
    28081 aggtcaacag cacggcgagc agctaaagcg cggccttaaa aaccgccata ttcagcttat
    28141 cgcgctgggt ggcgcgatag ggacagggct attcctgggt agcgcctccg taatacagtc
    28201 cgcagggcca gggattatcc tgggttacgc cattgctggt tttatcgcct ttctgatcat
    28261 gcgtcagctg ggtgaaatgg tggtcgaaga acctgtcgca ggctccttta gccactttgc
    28321 ttataaatac tggggcagtt ttgccggttt cgcctctggc tggaactact gggtactgta
    28381 cgttttagtt gccatggcag agctgactgc cgtgggtaaa tacattcagt tctggtatcc
    28441 ggaaatccca acctgggttt ctgccgccgt attctttgtg gtgattaacg ccatcaacct
    28501 gaccaacgtt aaagtgtttg gcgagatgga gttctggttt gccattatca aagttattgc
    28561 ggtagtagcg atgatcatct tcggcggctg gctgctgttc agtggcaacg gcggcccgca
    28621 ggcgagcgtt agcaacctgt gggatcaagg tggtttcctg ccgcacggct tcaccggact
    28681 ggtgatgatg atggcgatta tcatgttctc gttcggtggt ctggaactgg tggggatcac
    28741 cgcagcagaa gctgataacc cggagcaaag tatcccgaaa gcaactaacc aggttatcta
    28801 ccgcatcctg attttctata ttggttcgtt agccgttctg ctctcactga tgccgtggac
    28861 ccgcgttacc gccgatacca gtccgtttgt gctgatcttc cacgagttag gcgatacctt
    28921 tgtggcgaat gcgctgaaca tcgtggtact gactgcggcg ctctccgtgt acaacagctg
    28981 cgtatattgc aacagccgta tgctgtttgg tctggcacaa caggggaacg cgccaaaagc
    29041 gctggcgtct gtcgataaac gcggcgtacc ggttaacacc attctggtgt ctgcgctggt
    29101 tacagcattg tgcgtattga ttaactatct tgctccggaa tccgcatttg gcctgttaat
    29161 ggcactggtg gtatccgcac tggtgatcaa ctgggcgatg atcagtctgg cgcatatgaa
    29221 gttccgtcgc gccaagcagg aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc
    29281 gctgggtaac tggatctgcc tgctgtttat ggcggcggta ctggtgatta tgctgatgac
    29341 cccaggaatg gcgatttcgg tatacctgat cccggtatgg ctgatcgtgt taggtatcgg
    29401 ctatctgttt aaagagaaaa ccgccaaagc cgtaaaagcg cattaatctc tctacgccct
    29461 cacccgtaca gggtgagggc aataatctct acaccaacgc cccgtaaatc gtcagtagcg
    29521 caatcaccac cacgaccacg aacgaggttt tcttcgccat tgaaaccgct gccttcggcg
    29581 tctccacctt atcgacatgc ggttcacgcg ccagagagaa ctgcgccaga cgcgttaata
    29641 cctgatattg cgaagtatgg aaatcaccca gcgaagcaaa ccaggccggt aacgctttct
    29701 caccatgacc gatcaaggca tataccacac ccgcaagacg aaccggcacc caatccagta
    29761 catgaagcac tgcatcaatg ccagactgta aacgatgatg cggcgtctgg tatcgcgcca
    29821 gccagtattg ccatgcacgt aagaacgcat accccatcag cgtaacgggt ccccaggttc
    29881 cccccacaat cagccagaac agcggtgcaa gataaaaacg aaagttaatc cacagcaatg
    29941 cattttgcag ctcacgcaaa tactcacgtt cgtcgcagcc tgccgggacg ccgtgaatca
    30001 tggtgagttc gccagccatc gtggcacggg catggctatc attacgtgaa gcagctgtca
    30061 gataagcatg ataatgaaga cgaactttac ctgcgccaat acacagcaaa ccaatcagca
    30121 gccacaccag cagcgtggga acgttgaaca atactccctg caatgcgcgt aacagtaaaa
    30181 aagtcacacc catcgcaata atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca
    30241 cccgccgaaa gaacgcttca agacgatgat caagctgcca gtgctcgccc aacttaaaca
    30301 ggcgctcgaa aattaacacc agtaaggttg taaatagcgt catgttgtct ccttgctgac
    30361 cagcgcacga aaccgtgccc aatcaaatgc aggaccggga tcggttttcc gatccggcgc
    30421 aatatcacaa tggcccgtca tgttattagc gatatcggga tagcgatcta tcagtgcccg
    30481 cgtaaccgcc gcaagctgtt gatactgcgc atcggtatac gccagagtat cggtgccttc
    30541 aagctcaatc ccaatagaaa aatcattgca gcgttcacgc ccctgatact gagagactcc
    30601 cgcatgccag gcacgtttat cgaaaggaac atactggact atttcaccat cacggcgaat
    30661 caaacagtga gcggagacgc gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg
    30721 atcaatagtt ccagtgaata atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag
    30781 gctaatattg tgcaccacca gcagggtggg tgtttcgtca tccaggcggc aatcgtaatg
    30841 tggtgaggga acgcggcgcg cgccaaccag ccacccctgt tctaacaaca tgcaggatct
    30901 ccttatatgt ggtgctaata cccggttcag agtagcatgt ttctacctta tgattcgtta
    30961 gctatctgga gttttaacat gccgcctcgc cgctacaacc ctgacacccg acgtgacgag
    31021 ctgctggaac gcattaatct cgatatcccc ggcgcggtgg cccaggcgct gcgggaagat
    31081 ttaggcggaa cagtcgatgc caacaatgac attacggcaa aacttttacc ggaaaattct
    31141 cgctctcatg ccacggtgat cacccgcgag aatggcgtat tttgtggcaa acgctgggtt
    31201 gaagaggtgt ttattcaact ggcaggcgac gatgtcacca taatctggca tgtggatgac
    31261 ggcgatgtca tcaacgccaa tcaacccttg ttcgaacttg aaggcccatc ccgcgtgctg
    31321 ttaacgggcg aacgcactgc gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag
    31381 gtacgccact atgtcgaatt actggaaggc accaacacgc agttgttgga tacgcgcaaa
    31441 accttacctg gcctgcgttc agctctgaaa tacgcggtac tttgcggcgg cggagcgaat
    31501 caccgtctgg ggctttctga tgccttcctg atcaaagaaa accatattat tgcctccggc
    31561 tcagtgcgcc aggcggtcga aaaagcgtcc tggctgcatc cggatgcgcc agtagaagtc
    31621 gaagtagaga atctggaaga acttgatgag gccctgaaag ccggagccga tatcatcatg
    31681 ctggataact tcgaaacaga acagatgcgc gaagccgtca aacgcaccaa cgacaaggcg
    31741 ctactggaag tgtctggcaa cgtcactgac aaaacactgc gcgaatttgc cgaaacgggc
    31801 gtggacttta tctccgtcgg tgcgctaact aaacacgtac aagcactcga cctttcaatg
    31861 cgttttcgct aattgctgac agatttatcc gctccatcgc gggcggataa tctccccttc
    31921 cccaactttc ttcgtaacgc ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa
    31981 cctcggaatc tgctttcccg tttcgttttt tgcccctcgc ctgtgctgat ggcgtttgcc
    32041 aaagtagcac caaccaaatc aaggagcgaa acagatggac aagcaacgcg gttttacact
    32101 tatcgaactg atggtggtta ttggcatcat tgccatttta agcgccattg gtattcccgc
    32161 ttatcaaaac tacctgcgca aagccgcact caccgacatg ctacaaacct ttgtgcctta
    32221 ccgtaccgcc gtagagttgt gcgcgctgga acatggtgga ttagatacct gcgacggtgg
    32281 cagcaatggc attccctcgc ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa
    32341 gggcgtggtg tcgctgaccg ggcaagaaag tctcaatggg ctaagcgtcg tcatgacgcc
    32401 aggttgggat aacgcaaacg gcgtcaccgg ctgggcgcgc aactgcaata ttcaaagtga
    32461 cagcgcattg cagcaagcct gcgaagatgt cttccgcttt gatgacgcca actaaggagc
    32521 ggcaatgaat attccacagc tcacggccct gtgcctgcgt tatcagggag tcttgctgga
    32581 tgccagcgaa gaagtggttc atgttgcggt ggtcgatgcc ccctcacatg agttgctgga
    32641 cgcattgcat ttcgctacca ccaaacgtat tgagatcacc tgctggacgc gccaacaaat
    32701 ggaaggtcac gccagtcgca cacaacagac attgcccgta gctgttcagg agaagcatca
    32761 gcccaaagca gagttgctga ctcgaacgtt acaatctgcg ctggaacaac gcgcgtctga
    32821 tattcatatc gaaccagcgg acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt
    32881 gcatccttta ccggatgttt caccggatgc cggagtcgca ttaacagcca gattaaaagt
    32941 gctgggaaac ctggatattg cggaacatcg cctgccgcag gacgggcaat tcactgtcga
    33001 actggcagga aacaccgtct catttcgtat tgcgacctta ccatgtcggg gtggtgaaaa
    33061 ggtggtatta aggttgttac agcaggtgag ccaggcactg gatgttaaca cgctgggaat
    33121 gcagccgtta caactggcgg actttgctca tgccttgcaa caaccacagg gactggtgct
    33181 ggtaactggc cctacaggca gcggcaaaac ggtcacgctt tatagtgccc tgcaaacgct
    33241 gaataccgct gacattaata tttgtagcgt cgaagatccg gttgagatcc ccatagccgg
    33301 actaaaccag acgcaaatcc atccgcgtgc cgggctcacc tttcagggtg ttttgcgtgc
    33361 gttattgcgc caggatcctg acgtcatcat gatcggagag atccgcgatg gcgaaacggc
    33421 agaaattgcc attaaagccg tgcaaaccgg tcacctggtg ttgtccacgc tacacaccaa
    33481 ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gttgcccgct ggatgctatc
    33541 atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg
    33601 ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca
    33661 ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt
    33721 tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga
    33781 atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct
    33841 ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca
    33901 tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg
    33961 ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta
    34021 ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg
    34081 cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc
    34141 tggctctgct ggcggaacag catcccagta aacaatggca agcgttgctg caatcgctgg
    34201 cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat
    34261 ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat
    34321 gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat
    34381 cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc
    34441 tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg
    34501 cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg
    34561 tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc
    34621 ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac
    34681 aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt
    34741 tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga
    34801 cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg
    34861 gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg
    34921 acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata
    34981 acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc
    35041 tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat
    35101 aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt
    35161 ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc
    35221 cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac
    35281 cgggcctcgc agcggcagct tgacggtttt accttctgct gcgcggtatt ccgcaacgcc
    35341 gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc
    35401 gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag
    35461 catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc
    35521 accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc
    35581 agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc
    35641 aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt
    35701 cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa
    35761 ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg
    35821 gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac
    35881 catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac
    35941 agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga
    36001 aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc
    36061 tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga
    36121 gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat
    36181 gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca
    36241 tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca
    36301 taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt
    36361 tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg
    36421 ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg
    36481 gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg
    36541 cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc
    36601 agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg
    36661 acctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa gcaacttccc
    36721 cctatgtact gtgggttgtg ccattgctga tagaaaactc actgtataaa aaagcgaatc
    36781 gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg
    36841 atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc
    36901 ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg
    36961 ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac
    37021 cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct
    37081 gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg
    37141 cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt
    37201 ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg
    37261 cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggg
    37321 gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc
    37381 tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt
    37441 gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc
    37501 cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc
    37561 ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga
    37621 cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa
    37681 gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg
    37741 tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa
    37801 ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg
    37861 ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc
    37921 cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt
    37981 gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc
    38041 aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta
    38101 ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac
    38161 cgggttgccc ttctttaccc cacggcaccc cttcccaact ttcgaccagc cagaaccaca
    38221 gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg
    38281 gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca
    38341 tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac
    38401 gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt
    38461 tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag
    38521 cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg
    38581 atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga
    38641 gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg
    38701 ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact
    38761 gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt
    38821 gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata
    38881 gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt
    38941 ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat
    39001 ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag
    39061 ccactcggca attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg
    39121 aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa
    39181 cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt
    39241 acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg
    39301 cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt
    39361 cacccacggg tgttcaatgg cttcgcctag cttcataccc agtttacgca tcatgccgga
    39421 tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga
    39481 agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga
    39541 ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg
    39601 tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc
    39661 aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt
    39721 cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg
    39781 gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag
    39841 ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc
    39901 tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg
    39961 cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat
    40021 ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata
    40081 cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg
    40141 cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat
    40201 ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac
    40261 gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat
    40321 gttggccgga gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc
    40381 ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga
    40441 gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg
    40501 aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat
    40561 cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg
    40621 cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa
    40681 gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg
    40741 tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg
    40801 gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt
    40861 accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt
    40921 acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa
    40981 gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat
    41041 cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt
    41101 cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac
    41161 tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt
    41221 gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg
    41281 tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc
    41341 agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct
    41401 tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat
    41461 gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga
    41521 aagaaagatg acggattacc gtgcgaatag catgttgatg ccagtaatca acggaatagt
    41581 tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg
    41641 ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc
    41701 tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt
    41761 agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat
    41821 cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga
    41881 atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag
    41941 ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg
    42001 ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt
    42061 gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt
    42121 cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa
    42181 tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg
    42241 acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac
    42301 aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat
    42361 atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga
    42421 gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt
    42481 gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac
    42541 acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc
    42601 gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc
    42661 gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc
    42721 tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg
    42781 cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat
    42841 gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa
    42901 accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc
    42961 tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa
    43021 agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg
    43081 ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag
    43141 caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca
    43201 ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga
    43261 tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac
    43321 cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga
    43381 actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag
    43441 acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac
    43501 ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg
    43561 tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac
    43621 cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac
    43681 cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct
    43741 tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga
    43801 aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga
    43861 ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg
    43921 caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt
    43981 ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc
    44041 caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt
    44101 caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga
    44161 ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg
    44221 tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca
    44281 gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt
    44341 cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat
    44401 aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca
    44461 cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac
    44521 cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct
    44581 gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca
    44641 cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt
    44701 ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg
    44761 cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc
    44821 cagcataagg aattacctta gtgtggcgta atgccccacc ggtataaacg gcgatatcca
    44881 ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata
    44941 ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt
    45001 caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg
    45061 cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg
    45121 catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg
    45181 cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca
    45241 taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga
    45301 tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg
    45361 attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa
    45421 tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac
    45481 caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg
    45541 tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc
    45601 tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg
    45661 ctgtaaaacc ggataaagtt ctacaaagcg agccaaacgt ttcatcgtat cgccccggcc
    45721 aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc
    45781 cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg
    45841 caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt
    45901 gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca
    45961 ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct
    46021 gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac
    46081 atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc
    46141 attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc
    46201 atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag
    46261 caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc
    46321 gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta
    46381 ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc
    46441 tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca
    46501 taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg
    46561 cctgcaaatt ggcctcttgt gacgctttca gacctgcggg gcagaaatac tgtgtctcat
    46621 cagagagata cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg
    46681 gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa
    46741 tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg
    46801 ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca
    46861 aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc
    46921 gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc
    46981 gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca
    47041 tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa
    47101 acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag
    47161 gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag
    47221 aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt
    47281 tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg
    47341 atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc
    47401 tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt
    47461 gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact
    47521 tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg
    47581 cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct
    47641 gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc
    47701 agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca
    47761 aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt
    47821 ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc
    47881 gcattcaggg tgacgcgcat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc
    47941 gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt
    48001 gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc
    48061 accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc
    48121 tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc
    48181 atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga
    48241 ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt
    48301 gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg
    48361 tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag catttcggca
    48421 cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa
    48481 atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa
    48541 taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa
    48601 ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat accggcacca
    48661 ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt
    48721 tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca
    48781 aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg
    48841 tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg
    48901 accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcgataattt
    48961 tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt
    49021 gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac
    49081 cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt
    49141 cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg
    49201 aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg
    49261 gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg
    49321 gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca
    49381 ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg
    49441 tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta
    49501 caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca
    49561 taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccagccgt
    49621 tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga
    49681 tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg
    49741 cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc
    49801 ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac
    49861 tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg
    49921 caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag
    49981 cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc
    50041 ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat
    50101 ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag
    50161 tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt
    50221 gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag
    50281 ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc
    50341 ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag
    50401 caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc
    50461 cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg
    50521 ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg
    50581 caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag
    50641 cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga
    50701 tgccccttta acagcgctac ctactaccag cacgatcatc agcaggatga tagatccgag
    50761 cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc
    50821 cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt
    50881 ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc
    50941 gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt
    51001 ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca agatactgaa
    51061 tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc
    51121 gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca
    51181 caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata
    51241 gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg
    51301 ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg cgccagtggg
    51361 ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc
    51421 aggccattca gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag
    51481 cgcacaccgt tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt
    51541 aacgctttca ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccgcc
    51601 agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc
    51661 tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg
    51721 ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca
    51781 tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac
    51841 tgttgtaaac caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct
    51901 gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac
    51961 agttcacact catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca
    52021 acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg
    52081 ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg
    52141 ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata
    52201 ccgggactgg cgacaatcag atctgccgcc atcaaccatt catcattcag actgcccgtg
    52261 tggcgttcta cggcttcggg taatttatcc aggctaggcg gtgtcatacg cgtatccata
    52321 acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc
    52381 aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg
    52441 ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg
    52501 gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt
    52561 gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca
    52621 cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg
    52681 caccacctaa cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa
    52741 accacaggaa gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg
    52801 cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg
    52861 ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac
    52921 catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt
    52981 agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg
    53041 gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc
    53101 agaaatattt ccaacgagcg atcaaccctt tggtatcttt acgcaccact ttgcgataat
    53161 catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg
    53221 gattggacgg gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc
    53281 cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta
    53341 cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc cacaatgaga
    53401 tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa
    53461 agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg
    53521 taaagcgcgt actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat
    53581 tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa
    53641 atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac
    53701 gcggtcaata ccagcagctt ttgccgcctc gcccacctgt acatggcagt cttcgctttc
    53761 agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag
    53821 tacctgaaca gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa
    53881 ctggttttct gccagttgga tggggaacag acggccagga acagctttca gatttgccag
    53941 ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag
    54001 cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg
    54061 ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc
    54121 gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca
    54181 gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct
    54241 aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc
    54301 cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc agactcacag tcctggcaat
    54361 ctcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg
    54421 caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt
    54481 gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc
    54541 cgtcagagca accacgcgcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa
    54601 cgccagacgc gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac
    54661 cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg catcaaaacg
    54721 ttcacctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac
    54781 agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct
    54841 aagggttacg ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag
    54901 tagtccagac gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt
    54961 accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc
    55021 atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc
    55081 ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca
    55141 attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac
    55201 cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc
    55261 gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt
    55321 ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc
    55381 gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg
    55441 ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga
    55501 tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc
    55561 attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg
    55621 gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg
    55681 tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac
    55741 gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt
    55801 gcgcaaaaag tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc
    55861 gaaccggtag tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc
    55921 attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg
    55981 gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa
    56041 aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg
    56101 ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca
    56161 gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact
    56221 acagctacaa agagatcgcc cgccgcagcc acacggctgt cgagtgtcat ctctcgcagt
    56281 gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc
    56341 acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc
    56401 cggctcgatg ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga
    56461 aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa
    56521 gcgcggctga ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg
    56581 accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat
    56641 cgccgccttc acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt
    56701 gcgaacaatg gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat
    56761 cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa
    56821 cggtgttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg
    56881 ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc
    56941 cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgcta acgccagctt
    57001 ggaaacaccg acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg
    57061 tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt
    57121 ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc
    57181 cggttcaaac acgtcggtga tggtacggtt acgcatcgcc tctttcggcg tgccgctcag
    57241 attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc
    57301 caccagcacg gcgctaccag attcagcctt gttaaaggcc accgcattgt tcagttcgcg
    57361 ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct
    57421 gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc
    57481 acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact
    57541 atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg
    57601 agactcttca cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc
    57661 agagtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc
    57721 agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac
    57781 gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac
    57841 gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg
    57901 ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg
    57961 taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa
    58021 cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt
    58081 cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga
    58141 cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc
    58201 gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc
    58261 cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac
    58321 ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa
    58381 aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt
    58441 aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc
    58501 agaactgaac tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg
    58561 cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac
    58621 cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct
    58681 tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag
    58741 ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg
    58801 aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact
    58861 tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg
    58921 gcaatgcgtt tggcaaaacg ctcttcacca taggttttca atacccaggc gatatcggct
    58981 tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg
    59041 tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa
    59101 gagacgccaa gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg
    59161 tattcgccca gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc
    59221 ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca
    59281 agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg
    59341 ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta
    59401 tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc
    59461 caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag
    59521 ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg
    59581 cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc
    59641 taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcgacagac gcgataattt
    59701 ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc
    59761 aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg
    59821 cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc
    59881 ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga
    59941 ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc
    60001 taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc
    60061 tgtaagaaaa aatccctcat cgtcatcact gttttaaaat gactcactat tttccacagc
    60121 aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt ccccggttaa
    60181 cagtccttgt tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa agcaaagaaa
    60241 agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa aaacgcgctg
    60301 tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaacggg cgggtttaaa
    60361 accatttatc aataatagtc aggaatagtc ttatttactt taaggatatt gat
//
LOCUS       DAJTNM010000011        58109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTNM010000011 DAJTNM010000000
VERSION     DAJTNM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.011"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(26..280)
                     /locus_tag="OO172_000786"
     CDS             complement(26..280)
                     /locus_tag="OO172_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5511070.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            435..1007
                     /gene="yeiP"
                     /locus_tag="OO172_000787"
     CDS             435..1007
                     /gene="yeiP"
                     /locus_tag="OO172_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5511071.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1230..2696
                     /locus_tag="OO172_000788"
     CDS             1230..2696
                     /locus_tag="OO172_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5511072.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNPLKI"
     gene            2814..3800
                     /gene="yeiR"
                     /locus_tag="OO172_000789"
     CDS             2814..3800
                     /gene="yeiR"
                     /locus_tag="OO172_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5511073.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3839..4552
                     /gene="lpxT"
                     /locus_tag="OO172_000790"
     CDS             3839..4552
                     /gene="lpxT"
                     /locus_tag="OO172_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5511074.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4964..5530
                     /gene="mepS"
                     /locus_tag="OO172_000791"
     CDS             4964..5530
                     /gene="mepS"
                     /locus_tag="OO172_000791"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5511075.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5711..7267
                     /locus_tag="OO172_000792"
     CDS             5711..7267
                     /locus_tag="OO172_000792"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5511076.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7349..9163
                     /locus_tag="OO172_000793"
     CDS             7349..9163
                     /locus_tag="OO172_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5511077.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9164..10258
                     /locus_tag="OO172_000794"
     CDS             9164..10258
                     /locus_tag="OO172_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5511078.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10258..11283
                     /locus_tag="OO172_000795"
     CDS             10258..11283
                     /locus_tag="OO172_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5511079.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11285..12874
                     /gene="yejF"
                     /locus_tag="OO172_000796"
     CDS             11285..12874
                     /gene="yejF"
                     /locus_tag="OO172_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5511080.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     LSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12878..13222)
                     /locus_tag="OO172_000797"
     CDS             complement(12878..13222)
                     /locus_tag="OO172_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511081.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO172_000798"
     CDS             complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO172_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5511082.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO172_000799"
     CDS             complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO172_000799"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5511083.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15618..17378
                     /locus_tag="OO172_000800"
     CDS             15618..17378
                     /locus_tag="OO172_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5511084.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17503..17787
                     /gene="rplY"
                     /locus_tag="OO172_000801"
     CDS             17503..17787
                     /gene="rplY"
                     /locus_tag="OO172_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5511085.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO172_000802"
     CDS             complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO172_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5511086.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19115..19342
                     /locus_tag="OO172_000803"
     CDS             19115..19342
                     /locus_tag="OO172_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5511087.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19362..21122
                     /gene="pbgA"
                     /locus_tag="OO172_000804"
     CDS             19362..21122
                     /gene="pbgA"
                     /locus_tag="OO172_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5511088.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21197..21273
                     /locus_tag="OO172_000805"
     tRNA            21197..21273
                     /locus_tag="OO172_000805"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21231..21233,aa:Pro,seq:ggg)
     gene            complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO172_000806"
     CDS             complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO172_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5511089.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24287..24934
                     /gene="narP"
                     /locus_tag="OO172_000807"
     CDS             24287..24934
                     /gene="narP"
                     /locus_tag="OO172_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5511090.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO172_000808"
     CDS             complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO172_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5511091.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO172_000809"
     CDS             complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO172_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5511092.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO172_000810"
     CDS             complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO172_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5511093.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO172_000811"
     CDS             complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO172_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5511094.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO172_000812"
     CDS             complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO172_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5511095.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO172_000813"
     CDS             complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO172_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5511096.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO172_000814"
     CDS             complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO172_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5511097.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO172_000815"
     CDS             complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO172_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5511098.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO172_000816"
     CDS             complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO172_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5511099.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO172_000817"
     CDS             complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO172_000817"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5511100.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO172_000818"
     CDS             complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO172_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5511101.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO172_000819"
     CDS             complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO172_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5511102.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO172_000820"
     CDS             complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO172_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5511103.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTAMKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO172_000821"
     CDS             complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO172_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5511104.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO172_000822"
     CDS             complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO172_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5511105.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRVWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37692..38180
                     /gene="eco"
                     /locus_tag="OO172_000823"
     CDS             37692..38180
                     /gene="eco"
                     /locus_tag="OO172_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5511106.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38330..39361)
                     /locus_tag="OO172_000824"
     CDS             complement(38330..39361)
                     /locus_tag="OO172_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5511107.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQSTLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39595..39954)
                     /locus_tag="OO172_000825"
                     /pseudo
     CDS             complement(39595..39954)
                     /locus_tag="OO172_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40172..41815)
                     /gene="yojI"
                     /locus_tag="OO172_000826"
     CDS             complement(40172..41815)
                     /gene="yojI"
                     /locus_tag="OO172_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5511108.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41891..42485)
                     /gene="alkB"
                     /locus_tag="OO172_000827"
                     /pseudo
     CDS             complement(41891..42485)
                     /gene="alkB"
                     /locus_tag="OO172_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42485..43549)
                     /gene="ada"
                     /locus_tag="OO172_000828"
     CDS             complement(42485..43549)
                     /gene="ada"
                     /locus_tag="OO172_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5511109.1"
                     /translation="MKNATCLTDDQRWQSVLARDPDADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43623..44678)
                     /gene="apbE"
                     /locus_tag="OO172_000829"
     CDS             complement(43623..44678)
                     /gene="apbE"
                     /locus_tag="OO172_000829"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5511110.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44790..45911)
                     /gene="ompC"
                     /locus_tag="OO172_000830"
     CDS             complement(44790..45911)
                     /gene="ompC"
                     /locus_tag="OO172_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5511111.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46649..49321
                     /gene="rcsD"
                     /locus_tag="OO172_000831"
     CDS             46649..49321
                     /gene="rcsD"
                     /locus_tag="OO172_000831"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5511112.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQILIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            49338..49988
                     /gene="rcsB"
                     /locus_tag="OO172_000832"
     CDS             49338..49988
                     /gene="rcsB"
                     /locus_tag="OO172_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5511113.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50188..53037)
                     /gene="rcsC"
                     /locus_tag="OO172_000833"
     CDS             complement(50188..53037)
                     /gene="rcsC"
                     /locus_tag="OO172_000833"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5511114.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKKTLTVYAERVRKSRDS"
     gene            complement(53312..54088)
                     /locus_tag="OO172_000834"
     CDS             complement(53312..54088)
                     /locus_tag="OO172_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5511115.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDMDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54093..55742)
                     /locus_tag="OO172_000835"
     CDS             complement(54093..55742)
                     /locus_tag="OO172_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5511116.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55743..56465)
                     /locus_tag="OO172_000836"
     CDS             complement(55743..56465)
                     /locus_tag="OO172_000836"
                     /inference="COORDINATES: protein motif:HMM:NF036531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511117.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEE
                     EMSFTLQPVTSNEIDSDALYLDEITLTCEQDAVLRYGQVEVPLPPGADVERTTWGISV
                     NKPNAAEQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYV
                     RSYAPAQQSVAAGSEWTGMQVK"
     gene            complement(56462..58021)
                     /locus_tag="OO172_000837"
     CDS             complement(56462..58021)
                     /locus_tag="OO172_000837"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511118.1"
                     /translation="MTTSELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFY
                     PLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMPERPRREEVDTAAWMPS
                     LTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVY
                     RVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLS
                     AELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETP
                     QEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRL
                     MQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQA
                     DNMPLLHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILD
                     TPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGG
                     DATQTAAILMV"
BASE COUNT        14189 a        15683 c        14289 g        13948 t
ORIGIN      
        1 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
       61 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
      121 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
      181 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
      241 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
      301 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
      361 ccacttgccc gaaaggccca tcgcgagtac tttgtcgcga tatttttgac attttcgact
      421 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
      481 acggcaaact gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg
      541 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
      601 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
      661 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
      721 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
      781 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
      841 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
      901 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
      961 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
     1021 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
     1081 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
     1141 atcgcattaa caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt
     1201 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
     1261 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
     1321 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
     1381 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
     1441 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
     1501 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
     1561 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
     1621 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
     1681 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
     1741 gccgccgtga gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
     1801 atggccatgt ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg
     1861 ccgggtggat aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
     1921 ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg
     1981 cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc
     2041 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
     2101 aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
     2161 tcgcctatat cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa
     2221 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
     2281 ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga
     2341 tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc
     2401 gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg
     2461 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
     2521 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
     2581 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
     2641 aacaaatcgt acaattcggc gcacatcagg cgctattaaa ccccctcaaa atttaacgat
     2701 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct
     2761 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
     2821 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
     2881 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
     2941 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
     3001 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
     3061 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagctcctcg
     3121 atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc
     3181 tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
     3241 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
     3301 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
     3361 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
     3421 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
     3481 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
     3541 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
     3601 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
     3661 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
     3721 agctgatttc cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc
     3781 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
     3841 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc
     3901 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
     3961 ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgactaacaa
     4021 tcgcgccttc gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct
     4081 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
     4141 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
     4201 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
     4261 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
     4321 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc
     4381 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
     4441 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
     4501 cttttttgac aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt
     4561 aattaacatc atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
     4621 ttcatctatc gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
     4681 tcaattgtac tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata
     4741 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt
     4801 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
     4861 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcctgg cattttttta
     4921 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
     4981 gattttgaga tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc
     5041 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcaa tgggtagtga
     5101 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
     5161 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
     5221 cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt
     5281 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
     5341 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
     5401 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
     5461 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
     5521 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
     5581 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
     5641 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
     5701 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
     5761 tgtagcaggc gtgatgattg cgatactggt tagctgcctt cagtttttag tggccaggca
     5821 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
     5881 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
     5941 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
     6001 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
     6061 caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg
     6121 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
     6181 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
     6241 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
     6301 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat
     6361 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
     6421 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct
     6481 gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt
     6541 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt
     6601 actgctacgc tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt
     6661 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
     6721 cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc
     6781 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
     6841 gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
     6901 agaagccaca caactcttcg cctggctgca cttggtcggc gtagaaattg ctattgatga
     6961 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
     7021 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
     7081 cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac
     7141 gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
     7201 tagtcgcccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca
     7261 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
     7321 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
     7381 tctgttcacc ttcggtgcgc aggcgcaggc tatcaaggaa agctatacct ttgccgtgct
     7441 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
     7501 aaaaggtggg cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc
     7561 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc
     7621 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga
     7681 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat
     7741 tactgcccgc gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt
     7801 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
     7861 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
     7921 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
     7981 tccgtaccac gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
     8041 ttactgggca gcaaacttac cggtaaaccg tggacgctgg catttcgaca ccattcgcta
     8101 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
     8161 gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa
     8221 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
     8281 gtttaatatc caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc
     8341 ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag
     8401 ttattttcaa aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
     8461 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
     8521 ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
     8581 cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct
     8641 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
     8701 cagcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac
     8761 taaccgcatg cgcaatcgcg actatgacat gatgccgcgc ctatggcggg cgatgccgtg
     8821 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
     8881 ccccggcgtg caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg
     8941 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
     9001 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
     9061 ccaacccgct gtacgccctg tttacagcct aggtatcgat acctggtggt atgacgttaa
     9121 taaagcggcc aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat
     9181 tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat
     9241 cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa
     9301 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
     9361 tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat
     9421 cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
     9481 gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg cgtcggtgct
     9541 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
     9601 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt
     9661 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
     9721 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
     9781 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
     9841 gcatatcacg ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct
     9901 gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
     9961 aggggtaagt gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt
    10021 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    10081 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    10141 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    10201 tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg
    10261 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc
    10321 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    10381 gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    10441 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    10501 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    10561 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    10621 tggctgggaa cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg
    10681 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    10741 ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa
    10801 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    10861 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    10921 gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt
    10981 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    11041 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc
    11101 ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    11161 aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    11221 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    11281 tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca
    11341 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    11401 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    11461 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    11521 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    11581 ggaaccgatg ctgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    11641 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    11701 tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    11761 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    11821 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    11881 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    11941 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    12001 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    12061 tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga
    12121 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    12181 tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg
    12241 cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca
    12301 gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc
    12361 tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    12421 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    12481 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    12541 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    12601 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    12661 ggtacaggcg caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta
    12721 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    12781 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    12841 ggagtatacg cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    12901 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    12961 ggcgatttac aaacaggcac ggctcacctt cacactccaa caccgaacat ggaacttcga
    13021 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    13081 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt
    13141 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    13201 ttgagagttg taatgaagtc acttattttc cccggaagta cattcgcttc acaatttgtt
    13261 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    13321 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    13381 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    13441 actttttaca taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg
    13501 tggttgacat gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg
    13561 ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    13621 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    13681 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    13741 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    13801 caccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat
    13861 ccacgctgcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    13921 gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc
    13981 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc
    14041 ggcgcttaag aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag
    14101 gacacgttta tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg
    14161 ctgacgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag
    14221 aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    14281 gccgccaact atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat
    14341 catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct
    14401 ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg
    14461 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg
    14521 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    14581 aatataagta ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat
    14641 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    14701 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    14761 gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    14821 gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac
    14881 gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    14941 cagacgaacc tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc
    15001 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc
    15061 cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    15121 ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc
    15181 gtgcagtttc cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc
    15241 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg
    15301 ctgcgccagc gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc
    15361 attacgaacg atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    15421 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    15481 gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta
    15541 tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa
    15601 tctacctacg cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg
    15661 ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg
    15721 caggtaaaag cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc
    15781 tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg
    15841 aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg
    15901 gcagcgttca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga
    15961 ttgtcgatga atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca
    16021 ctcacctgac taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc
    16081 gattgggcaa aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga
    16141 aagccctttt ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct
    16201 atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc
    16261 aggcacagag taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc
    16321 aacgcattac gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg
    16381 taatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg
    16441 aagatgctgc actgattact ggcgaccccc ccggcgctga gcgcgatgtg ttaattgaag
    16501 attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg
    16561 acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc
    16621 aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg
    16681 attatgcggg taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa
    16741 gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg
    16801 ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt
    16861 ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc
    16921 cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac
    16981 tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac
    17041 gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca
    17101 cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc
    17161 agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc
    17221 tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt
    17281 ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg
    17341 aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata
    17401 aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt
    17461 tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca
    17521 gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc
    17581 ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa
    17641 gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctgaccat cgttgttgac
    17701 ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg
    17761 cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg
    17821 cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca
    17881 gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga
    17941 cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc
    18001 ggttgctgga tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa
    18061 gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa
    18121 gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac
    18181 gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc
    18241 attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc
    18301 ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa
    18361 tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc
    18421 tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat
    18481 acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt
    18541 ttcgttaacg cgcatactgc tcaggttgct cagaactgcc accagcagat actccaccgc
    18601 cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa
    18661 ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg
    18721 acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt
    18781 atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg
    18841 ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg
    18901 gtgcagagca atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg
    18961 tattcaacca ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca
    19021 gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata
    19081 tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa
    19141 tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc
    19201 gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat
    19261 tgccccggcc caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat
    19321 caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc
    19381 gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata
    19441 ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc
    19501 ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc ctggtgttcg
    19561 ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga
    19621 ggtttttgtc cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag
    19681 tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag
    19741 acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat
    19801 tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc
    19861 gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg
    19921 tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc
    19981 cgctttcgta cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc
    20041 aggagtatca acgccgtctt attgagcaag gtaatccaga caccgtttcc gtacagtatc
    20101 cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg
    20161 tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg
    20221 agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct
    20281 ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc
    20341 ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag
    20401 atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg
    20461 tacgcaccca atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg
    20521 cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca
    20581 gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga
    20641 tcaaacgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta
    20701 tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg
    20761 gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg
    20821 ctctgactga tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca
    20881 cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact
    20941 gggttactgc tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc
    21001 tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa
    21061 aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact
    21121 gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt
    21181 attagcagcc acgagtcggc acgtagcgca gcttggtagc gcaccgtcat ggggtgtcgg
    21241 gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta
    21301 cggttggttt ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct
    21361 gtttattttc tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc
    21421 tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga
    21481 tgaccttatc ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc
    21541 atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag
    21601 ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc
    21661 ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta
    21721 gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc
    21781 gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc
    21841 aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt
    21901 caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt
    21961 atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc
    22021 aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag
    22081 cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg
    22141 ggtgttaatt gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag
    22201 acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt
    22261 cagcacatcg gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg
    22321 gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt
    22381 gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc
    22441 tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt
    22501 gacgttgagc tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt
    22561 tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga
    22621 acgagtcact ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa
    22681 gttgaggcga gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt
    22741 ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc
    22801 ctcagaaacc actaccttac ccaccaacat agcgggtgca taatttattt gcggcttcat
    22861 gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac
    22921 attagcgcgg ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat
    22981 acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc
    23041 agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtgt caatagcctg
    23101 cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc
    23161 atcctttata ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga
    23221 gacaattaat ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac
    23281 ctccagtaag ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg
    23341 caaacttccg ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg
    23401 accgagacgg tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc
    23461 tgctttctga tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt
    23521 ttcgatagcg gcaaaaacgg tccccccatc tttttcaacg attaagcgcc cgcctttaat
    23581 cgtggtatca ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt
    23641 tgccgcgcca cccatttgaa caatttgata accatcattt aatactgagt attctgcggt
    23701 accattcatt tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa
    23761 ttcttttata tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac
    23821 cagctggata tctttttcca ggataacacc atcaactgtc tcaccatact caactgttga
    23881 tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    23941 aagagaaaca gaaccagatt gatgcattga gctttcatcc tatgaaatta attgcagtta
    24001 aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg
    24061 tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct
    24121 gatgcaaaat tccgtcttta taatgaaaat gatgccaaag cgaacgacaa ggttgtagtt
    24181 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac
    24241 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    24301 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    24361 actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgacggcg cgagcgcgat
    24421 cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat
    24481 gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    24541 cctgaccgta tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg
    24601 ttatctgttg aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa
    24661 aggcaacaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg
    24721 cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct
    24781 ggcacagggg ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt
    24841 aaaagtacat attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac
    24901 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg
    24961 gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg
    25021 atcgatcaat ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat
    25081 ccggcataaa ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct
    25141 ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa
    25201 tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa
    25261 cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga
    25321 tattgctatg gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt
    25381 tgggatcaga tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg
    25441 gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca
    25501 tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag
    25561 tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt
    25621 ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct
    25681 gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac
    25741 ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg
    25801 cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc
    25861 caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt
    25921 aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac
    25981 cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat
    26041 cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact
    26101 gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg
    26161 agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt
    26221 ctcccacagc ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg
    26281 atagcgaatg ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag
    26341 atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc
    26401 tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat
    26461 gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt
    26521 cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg
    26581 ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga
    26641 ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc
    26701 ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc
    26761 ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt
    26821 ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa
    26881 agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc
    26941 gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt
    27001 ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca
    27061 ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca
    27121 tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg
    27181 ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa
    27241 taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc
    27301 accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg
    27361 aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa
    27421 ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt
    27481 agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa
    27541 atactgccca gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc
    27601 agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc
    27661 gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg
    27721 ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac
    27781 gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag
    27841 aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc
    27901 gccagtgagt gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc
    27961 ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc
    28021 acaatgccga gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca
    28081 taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag
    28141 aaacccacgt accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac
    28201 agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga gaacgggtta
    28261 gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca
    28321 cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg
    28381 ctcatcagca gcacccacag cagaagcgaa ccttcatgcg cgccccaggt agccgccacg
    28441 cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg
    28501 ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc
    28561 caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc
    28621 ggatacacgg acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt
    28681 tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca
    28741 tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg
    28801 cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga
    28861 acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg
    28921 tgaaggtcac tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc
    28981 caacgcgcag acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa
    29041 tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga
    29101 tagtcagcgc cagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa
    29161 tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg
    29221 cgccacacca cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag
    29281 cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa
    29341 aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca
    29401 ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc
    29461 agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc
    29521 ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg
    29581 gagtaatgaa taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgg
    29641 cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac
    29701 agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt
    29761 tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc
    29821 atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc
    29881 accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata
    29941 atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag
    30001 ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt
    30061 tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca
    30121 gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg
    30181 tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc
    30241 catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg
    30301 ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg
    30361 tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca
    30421 gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa
    30481 ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg
    30541 cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac
    30601 ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg
    30661 gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta
    30721 caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca
    30781 gttcaagacg gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg
    30841 gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc
    30901 ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc
    30961 ggtaaaaggc tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc
    31021 gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag
    31081 cccggcctgc gccaaggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg
    31141 cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag
    31201 gttttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc
    31261 agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt
    31321 gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa
    31381 ggttcgttca tcccgctcac aaagtacctc tctggcttca agcataccca cgcaataacc
    31441 ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg
    31501 gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga
    31561 tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt
    31621 attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt
    31681 ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt
    31741 gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac
    31801 gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat
    31861 ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc
    31921 cccccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg
    31981 gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt
    32041 tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat
    32101 tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac
    32161 ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc
    32221 agtggtgcga tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac
    32281 ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat
    32341 ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga
    32401 ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat
    32461 cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa
    32521 ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac
    32581 gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt
    32641 agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga
    32701 tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc
    32761 ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg
    32821 agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg
    32881 gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc
    32941 aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg
    33001 cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat
    33061 cgctttcccg ccaaggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg
    33121 gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg
    33181 gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga
    33241 ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg
    33301 tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt
    33361 gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag
    33421 tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc
    33481 gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg
    33541 cgtgttgcgc tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca
    33601 aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag
    33661 ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac
    33721 tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg
    33781 cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc
    33841 ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc
    33901 gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac
    33961 acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc
    34021 attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca
    34081 cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac
    34141 cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc
    34201 gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc
    34261 acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa
    34321 ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac
    34381 gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa
    34441 cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc
    34501 cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac
    34561 caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc
    34621 gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag
    34681 ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac
    34741 ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc
    34801 cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg
    34861 ccagagatcc gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg
    34921 gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa
    34981 gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg
    35041 atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt
    35101 acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc
    35161 atgaagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat
    35221 atcacgatgt ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc
    35281 cacttcacgc gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc
    35341 cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt tggcccacac
    35401 accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc
    35461 ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt
    35521 tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga
    35581 ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta
    35641 gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat
    35701 cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg
    35761 cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac
    35821 cgccacggtg acgttctggt tagagagacg acggttagtg atgcgtgacc agaggatcgg
    35881 gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc
    35941 atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc
    36001 catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt
    36061 ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc
    36121 cggccctttt tctttcatgg cggttttgaa tttctcttcc atcacatcga aggcctgatc
    36181 ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag
    36241 cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca
    36301 gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg
    36361 ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca
    36421 tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc
    36481 agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat
    36541 ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc
    36601 tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc
    36661 accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg
    36721 ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa
    36781 acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc
    36841 tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcgcctggt agatcccgga
    36901 gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc
    36961 aactgactga tacgccaggc acgcgtcccc gatggtgaac tgcaaatccc agaccctggt
    37021 gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca
    37081 ctcgttattt ttgaaattaa cgctcggata gccacccgtg ccgcgttgca gaatgttgtt
    37141 ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc
    37201 accggaccag ggcggacgga taccgttact ggctttgcgc cagcgaccag tgagtatgcc
    37261 ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga
    37321 gagcaaccaa atagaccgca taattaataa gccattttta tagccgctaa gatattaaag
    37381 gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg tagcaggatc aaaagagtgg
    37441 cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atggttataa acatatttat
    37501 atagcgatta attcaccaga gatatttctg cttgtttgct ccctcatcag aatttaacac
    37561 taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc aaattaataa
    37621 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg
    37681 aaagcaaaaa aatgaagacc attctacctg cagtattgtt tgccgctttc gctaccactt
    37741 ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
    37801 aaacaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
    37861 aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
    37921 ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
    37981 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
    38041 tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
    38101 tgtatacgcc agacaatgta gatgtgaaat accgcgtctg gaaggcggaa gagaaaattg
    38161 acaacgcgga agttcgctaa actgttgtga agtacggcac cccgttggtt ggataaggcg
    38221 gttacgccgc atccaacatc taacgcccga gccggttgcc tgatgcgacg ctggcgcgtc
    38281 ttatcagtat acgccacatc cggcatacca tgccggatgt ggcacatcat tacaacgcaa
    38341 tatccgccac ttctttttga acgggttgcg gtttcaactg cggtttcggc gtactatctg
    38401 ctgcttgcgg tttgtcgtat ttcagcccca gcacttcgct ggtgtactgc aactcgcgtt
    38461 ctgttgccgc tacatcaccg ttcagcttgc gtccataaga cggaacgatc gctttcaacg
    38521 tagactgcca ttgcgggctg gaaacgcgat cgccaaatac tttttccagc agattcaaca
    38581 taatcggcgc ggcggttgac gcccctggcg atgcccccag cagcgccgca atggtccctt
    38641 gctggtcact gaccacttcg gtacccagac gcagtacgcc acctttctcg gcatcacgct
    38701 tgataatctg cacacgctgc cccgcttgcc acaaacgcca gtcctctttt ttcgcttgcg
    38761 gatagtactc tttcaacgct tcaaaacgat cctcttcact caacatcacc tgactcacca
    38821 gatatttcac cagatcgaaa ttatccagcc cgacgtgcat catcggcatc acgttagagg
    38881 tggtggtgga actcatcaga tcccacaatg aaccgttttt gaggaatttg gttgagaagg
    38941 tggcaaatgg cccaaacagc actacgcgtt taccgtccag aacacgggta tcgatatgcg
    39001 gaaccgacat cggtggtgcg ccaacggatg ctttaccgta aactttcgcc agatggcgat
    39061 taaccacgtc tggattttcc gaaacaagga actgtccgcc caccgggaaa ccggcgtagt
    39121 ctttcgcttc cggaatcccc gattcctgta acagcttcag cgccgcaccg cccgcgccga
    39181 taaagacaaa tttggtgcgg atgttctgtg cagtgccgtt tttcagatcg gcaacggtaa
    39241 cggtccaggt gttatcgtca ttacgcttta gggcgcggac ttcgctgctg agttgcagcg
    39301 agaagttaga tttcttctgt aaggaagcaa ttaactggcg ggtgatctca ccatagttca
    39361 catcagtgcc aatttctgtt gcggatcgcg cccttccatc actaacggtg cccactcttt
    39421 gatctgcgcg tgatcttcag agtaacgcat accgcgaaac agcgagcttt gttgcaacgc
    39481 ggcgtaacgg gcgcgcagga aattaacgtt atcctcgccc cagacaaagc tcatatgcgg
    39541 aacggtattg ataaatgaac gcggagtacg cagcacgccg cgctcgacct ggtgcgccca
    39601 gaactggcgg gaaatctgaa atgcttcgtt aatggcgact gctttttcaa tactgatgct
    39661 gccatcggcg ttttgcgggg tgtagttcag ttccatcagt gcagaatgcc cggttccggc
    39721 gttgttccag ccgttcgaac tctcctgcgc gcccccctcc aggcgctcca ccatggtcat
    39781 cgaccattca ggctccagct cgcgtaaata ggtccccaac gtggcgctca taatgccgcc
    39841 gccaatcaac agtacatcag tttcctgctc ctcggacgct ttcgcttttg ccgccatcga
    39901 aacggcatta agccccacgg ccatcgagaa gagcatggca gtcacttttt tcatcttgtt
    39961 aatgccttac ttttagtcgc tttattgcag gtgagatttg cgcggcatca acggtaacac
    40021 ttaagtaaca acatttaaat aatgtttata aattatattc taattttaga aacattataa
    40081 tttcgttgat taattatagg gttattagaa atgaagggat ttttagcata ttgacctgac
    40141 ggcagcagtc cgccaggtca gcgtcgtaat attatgccgt ccgggcaacg gcatcacgcg
    40201 aagcggcatc gcgctcttcg cccgtcagct cgctaagttg cccattgcgc atttccagca
    40261 ggcggtcggc gtggataaag taatgatcat catgactgat agcgaaaata gttttaccca
    40321 tctcctgcat cagcggcagc aacacctgat aaaactcacg acggaagtgt ggatcctgat
    40381 ccgccgccca ttcatccagc aggataatat cgcgttcttc tgccagcgcc agcaacagcg
    40441 gcacgcgttt tttctgccct tttgataact tcaggttaac aatacgcccg ttgcttaact
    40501 caagcttatg agccattttc agctgcgcca gccacttctc aaccagttgc gggttagcgg
    40561 gttgcccctc cggccccagc agttgatcaa acagccagac atcggtaaac actgccgaaa
    40621 acagtttgcg ataatcttcc ggttgttcgc cgctgacagg tttgccatcc agcaagattt
    40681 cgccgctttg tggctgatac aagcccgtca acaacatcgc cagcgtcgat tttccgctac
    40741 cgttgccgcc aatcagaaac agcagctcgc cacgtttgat ggtgagatta atcggaccaa
    40801 cggaaaacgc gttatcctga taagcaaacg tcacgttacg cagctccagc gtttgccagt
    40861 tgggaaacgc ctgcgggcgc ggaaactcag ctttgaaagg cgcgagcgcg aatttgttca
    40921 gcttgttaaa cgccacctgc gccgtcagca gcgtcggcaa tgcgccaacc gccgaaagca
    40981 gcggcgtacg caggaataac agcgtcaacg aataggtcgc agcaacgttg gtatcagccc
    41041 aaccgagact gttcgccatc cagaacacca ggccgatagc gcccagcatc atgatgtttg
    41101 accagttcac ggcactaaga tggaaggtgt ctgcgcgaat aatatgatgg cgatactctt
    41161 gcgcatcggg gatgtagagg ttgttaaaca catactcggc gcgttcccgg ttcagagtca
    41221 gctctttgcg cccttcaagt acagtttgaa aatccgtgta cagcttgtct tcggtttcac
    41281 gtagggtcgc catatgtttg tacacccgcg ccaccagcac aaaaccgccc cagatggtga
    41341 tcgccatcca gatagccgtt accagcaaca ttttgcccga cagcatccac agatacgccg
    41401 atgaaccgat agtgagaatg atcccctgcc ccagttccgg cagacgcaca aaagcaatgg
    41461 tgatattgcg cacatcgctg gttaatcccg ccagcaacga ggcgctaccg agttgttcaa
    41521 tgcgctcgac gtgggtatcc agaatccgct tgataaattc actacgcagt cggtaaacga
    41581 agtgatgccc caaagtggtg agcgccagtt gcgatccgag agtgactgcc atcaacagca
    41641 gcaataatcc cagaaactcc ggtaacacca gcagactggt atccgccgtt tcgataaggc
    41701 gctgattgat aaaagcaatt aagccaatgc ctaatgccgc actggcgagg cttagcgcca
    41761 tcacactgat aaatggccag cgatactgcc gccagacaag tacaagaagt tccatgcaga
    41821 aaacccggac aatgaattac agcccgcagt ttaaacatct tcgcgcgcac agcaataata
    41881 attcttattt ttattctttt ttacctgcct gacggaatgt caggttgtag cggcagtcgg
    41941 tggtgagtgg atgaaacccc gctttcaacg gttgaatacc gtgataaaac agccgcgatt
    42001 caccgcccca ggccaccaaa ttgaaaaatc gcgggtaagc ccagagaaac agaaacaatt
    42061 ggcgcgcgca gatccggttc gtctttatcc tgatgcagcg acagtttcgc gctaggagcg
    42121 tagcggttga taagacaagc atctggctgg aaatctggat agcccgccgc cgtagccgca
    42181 cgttgacata aatcatgaaa gctttgtggc atggcgggcc acggtttatt tgtttgcgga
    42241 tcaatgggcg aatagagata accttgccga tgggtcgtcc agcccagatg cccacagttg
    42301 gtcatcgcca ccgacatggt atatccgcct ggagcgacca tctggcgaaa cggcgactgg
    42361 ctggcaacgt cattaatatc gcggattagt tgctccgcag cattaaaagc aaaacgccgt
    42421 aaaattaccg caccagccgc cagtggctct tgccacggtt cagcatcggc aaacagatcc
    42481 aacattacct ctcctcattt tcagcttcgc ggcgcagcag ttgcgcttta cgcgacacgc
    42541 cccagcggta accggaaagt gtgccatcac cacggaccac tcgatgacag ggtattacga
    42601 tagccagctt gttggcggca caggcgctgg caacggcccg taccgctttc ggtttgccga
    42661 tggcgttagc cagttgctga taactgacgg tttcaccaca aggtatcgtg cgcagtgcct
    42721 gccagacttg ttgctgaaaa gcagtgccgc gaatatccag cggtaacgtc agcggcgtat
    42781 cgcgttgatt gaggctggcg atcacttcac gcacatgttg ctgaaacgtc agatcggcag
    42841 gcgcgttgtc ggcagcggga aacatctgct gcaactcgct gattaatgtc gcgtcatcat
    42901 cgcccagcaa tatcgcgcaa atcccccgct cgctttctgc caccaggcaa cgacccagct
    42961 cacaatcagc cagcgcgtaa cgcaccgcca gattttcgcc accgtgacgg aattgtttag
    43021 ccgtcatgcc cagcgtttca tcggctttgc ggtagtaact gctgctgtcg gggaatccgg
    43081 cgttaagaat agacgtcgtc acgctctccc ctttcgccag cgattcgcgc aaacggcgag
    43141 cgcgccaggc ctgttgccag gctttaggcg tcattccggt agtagcttta aacaaccgat
    43201 gtagatgaaa tggactcatc gccacctggt cggctaaggc ttccagcgtt acaggcgttt
    43261 cctgttccag cagtcgacac gcgtgggtga ttttatccaa ccgatgttgc cggggattgg
    43321 ctttgtctgg ctgacaacgt ttgcaggggc gaaatccggc ggcgagtgcc tcgctggcat
    43381 ttgcgtagaa ggagacattt tcccgcaagg catgtctggc gcggcaagac ggacggcaaa
    43441 agatacccgt ggtgcgcacg gcgaaaacga attcgccgtc ggcatccggg tcgcgggcta
    43501 agacagattg ccagcgttga tcgtcagtta agcatgtggc gtttttcata atcagctccc
    43561 tggttaagga tagcctttag tctgcccggt caccatcacg caaaaaccaa caatcttgcg
    43621 ctttaatttt tttcgctgac aaggaagctt ttaaactgtg gtgacatcca ggttttaaag
    43681 ctatcgcctt ctttggtgat catataaacc gccagccctt cccggcgaac aacctctttg
    43741 gctttctccg gcccgagtac catcaagcca gtatcccagg catcggcttc cagcgccgtc
    43801 ggagcaatca ccgtcacgga taccagattg tgttcgatgg gacgcccggt ttgcggatcg
    43861 ataacatggg aaagacgttt gccgtccagt tcgtaatagt tacggtagct gccagaagtg
    43921 ctaatcccat gaccgttgat atccaccaca gcctgaaccg cgttttcttt atcggttggt
    43981 ttttgaatcg ctacccgcca cggctggcct tcaccgttca taccacggct gttcagcgcg
    44041 ccgcccaccg acaccagata gcgggaaatc ccttcctgct ccatcaagcg tgccaggtga
    44101 tccgccgcat aaccttcacc gacggtggag agatcgacat ataaatccgg caggtctttt
    44161 tgcagatatt gctgatgcga ctgattgatg accgtcaggt gctgtaagcc ggttttggct
    44221 ttcatcgcat cgatctgttc ctggctcgga atttgaaccg gctgttgttc cgggccaaag
    44281 ccccacagat tcaccagcgg cccgacggta atatccatcg caccatcggt ccttgcccca
    44341 atgcgcagcg aggtggtgac gatatcggcc atcgcttcac ttaccggcca cggcgacaaa
    44401 ctttgcgagt cgttaaagcg catcagcgcg gaatcttttt tataggtcga aagcagctga
    44461 tcgtcggcgt ccagctgggt ctgaatcttt tctttaagtt cggcactgcg tttggcatca
    44521 atgcccggga tgctggcacg ccagaaggta cccatggttt tgccttcaag aacggtaact
    44581 tcagtagcgt gggttttggc gggttgtggt ttttgatcgc aaccaacaaa gaagagcgcg
    44641 gcaaccagca gtgccacgcg ggtaaagctt atttccatac gtgattatcc tcatgcgaac
    44701 ggtcgcaaga gtacaccaaa aaactgtgtt tgtacgctga aaacaatgaa aaaagggccc
    44761 gcaggccctt tgttcgatat ccatcgagat tagaactggt aaaccagacc cagagctacg
    44821 atgttatcag tgttgatgcc agcgtcacga gtgaactggt tgtcgtccag caggttgatt
    44881 ttgtagtcaa cgtaggtgga catgtttttg ttgaagtagt aggtcgcacc aacatcaaca
    44941 tatttcagga tatcttcgtc gtcgtagtta cgaccattga tgacacccag gtttttacct
    45001 ttagactgca ggtaagctag ggacggacgc agaccgaagt cgaactggta ctgagcaaca
    45061 gcttcgaagt tctgtgcttt gttcgcccaa cccaagttgc ctacgcgagt tgcgttgtag
    45121 gtctgggtgt actgagcagc taggtagatg ttgttcgcgt cgtatttcag accaccggtg
    45181 taggtttcag cgcggtcgcc attaccgatg taacgctcat cataacggtt cagaccgaag
    45241 ttctggtcat cagtacgttt ggagctggaa actgcagcac cgataccgaa gccttcgtaa
    45301 tcataagtaa tagaaccgcc aacgccgtca ccgttctgac gcagagcttc acgaccgttg
    45361 ttggtcatgc cttcaccttc cgggctaccg tttttgccct gatactgaac agcgaagttc
    45421 agaccgtcaa ccagaccaaa gaagtcggtg ctacggtagg ttgcgaagcc gttaccacgc
    45481 tgctgcatga agttgtcaga accgtaagtg tcaccaccga attctggcag tacgtcggtc
    45541 caggaagtta cgtcgtaaac tacgccgtag ttacgaccgt agtcgaaaga acctacatcc
    45601 tggaatttca gacctgcgaa tgccacacgg gtccaggagt tgttttcgtt ttcagcgctg
    45661 ttgccctgga tctgatattc ccactggccg taaccggtca gctggtcagt aacctgagtt
    45721 tcacctttga agccaagacg catgtaggtc tggtcgccat ctacagactt gtcgtcagag
    45781 aaatagtgca ggccgtctac tttaccgtac agatctaatt tgttgccgtc tttgttgtaa
    45841 acttcagcag cgtttgctgc tcctgctacc agcagagctg ggaccaggag ggacagtact
    45901 ttaactttca tgttattaac cctctgttat atgcctttat ttgctttttt atgccactgc
    45961 atactgatta accctcatta atcagtcggc aagtccattc tccccaaaaa tgcagaataa
    46021 tccaacacga atatgatact aaaactttta agatgtttca tttatcgcta cagatgtttc
    46081 aaaatgtaaa tacaagggaa ctttttaaga ttattgcgga atggcgaaat aagcacctaa
    46141 catcaagcaa taataattca aggttaaaat caataactta ttcttaagta tttgacagca
    46201 ctgaatgtca aaacaaaacc ttcactcgca actagaataa ctcccgctat catcattaac
    46261 tttatttatt accgtcattc atttctgaat gtctgtttac ccctatttca accggatgcc
    46321 tcgcattcgg tttttttacc cttctttaca cacttttcat tattctgtgc taccacagaa
    46381 aaactataac gcttgttaac tatttcacaa ataattaaca tcagcataat ttccagcaat
    46441 ctttgtttat ttacaattaa tttcgttggg ctttttgtag gttatttgta cagcaaaatg
    46501 gcgcttgtac atctatttcc ccccatacag gatgataaat atcacgggag aatagagaat
    46561 catcaatcag gtaagagtct ggaatttcac actgtaccct ttatactgcc ctatcacttc
    46621 gcgaagtttt aacaggtcat aaacacgaat gcgtcagaaa gagacaacgg ccacgacccg
    46681 cttttcactc ctaccgggga gcattacccg cttcttttta ctgttgatca ttgtgttact
    46741 ggtgacgatg ggggtaatgg tacaaagtgc cgttaacgcc tggctgaaag ataaaagtta
    46801 ccagattgtc gacattaccc acgcaatcca aaagcgcgtc gatacctggc gttacgtgac
    46861 caggcagatc tacgacaaca ttgccgcgac gacctccccc tcctccggcg aaggtttaca
    46921 agagacgcgc ctgaaacagg atgtctacta tctggagaaa ccacgccgca aaacggaagc
    46981 gttaatcttt ggctctcacg acaactcaac gcttgagatg actcagcgga tgtccactta
    47041 tctggataca ttgtggggtg cagaaaatgt accgtggtcg atgtattacc tgaatggtca
    47101 ggataacagt ctggtgctga tctcaaccct accccttaaa gatctcacct ccggatttaa
    47161 agaatcgacc gtcagtgaca ttgttgattc acgtcgtgca gaaatgttgc aacaggccaa
    47221 cgccctcgat gaacgcgaaa gtttttctaa catgcgccgc ctggcctggc agaacggtca
    47281 ttactttacc ttgcgtacca cgttcaacca gccgggacat ctggcaacgg tcgtggcttt
    47341 tgatctgccg attaatgatt tgatcccacc gggtatgccg ctggacagtt tccgccttga
    47401 gccagacgcg acggcaacgg gaaacaatga taatgagaaa gaagggacgg atagcgtcag
    47461 tatccacttt aacagtacga agattgaaat ctcctcggca ctcaactcta ccgatatgcg
    47521 cctggtctgg caggttcctt atggcacctt attgctggat acgttgcaaa acattctgct
    47581 gccactgctg ctgaacatcg gtttgctggc gctggcgtta tttggctata ccacattccg
    47641 ccatttctcc agccgcagta cagaaagcgt ccccagcacg gcggtcaata acgaattgcg
    47701 cattttacgg gcaattaatg aagagatagt ctcactgttg ccgctcggcc tgctggttca
    47761 cgatcaggaa tcgaaccgca ctgtcataag taacaaaatt gccgatcatt tgctgccgca
    47821 tctgaatctg caaaacatca ccaccatggc ggaacagcat caggggatta ttcaggcgac
    47881 gatcaataac gagctgtatg agatccgcat gttccgcagc caggtcgcgc cgcgcacaca
    47941 aattctcatt attcgcgatc aggatcgcga agtgctggta aacaagaaac tcaagcaggc
    48001 gcagcgtctg tatgagaaaa accagcaggg gcggatgacc tttatgaaaa acattggcga
    48061 tgcgctgaaa gaacccgcac agtccctggc ggagagtgcg gctaaactca acgccccgga
    48121 aagcaaacaa ctggcgaatc aggcagatgt gctggtgcgg ctggtcgaag aaatacagtt
    48181 agcgaacatg cttgcggatg atagctggaa aagtgagacg gtgctgttct ccgtgcagga
    48241 tttaattgat gaagttgtgc cttcagtgtt gcctgccatc aagcgtaaag gtctgcaact
    48301 gctgattaac aatcatctga aagcacacga tatgcgccgc ggcgatcgcg atgccttacg
    48361 acgtattttg ctgctactga tgcaatatgc cgtgacctca acgcaattgg gaaaaatcac
    48421 ccttgaggtt gatcaggatg agtcctctga agaccgcctg acattccgca ttctggacac
    48481 gggagaaggc gtaagtattc atgaaatgga taatttgcac ttcccgttta tcaaccagac
    48541 ccaaaacgat cgttatggaa aggcggaccc gctggcattc tggctgagcg atcaactggc
    48601 acgtaaactg ggcggtcatt taaacatcaa aacgcgggat gggcttggta cacgctactc
    48661 tgtgcatatc aaaatgctcg ccgctgaccc ggaagttgaa gaggaagaag agcgtttact
    48721 ggatgatgtc tgcgtaatgg tggatgttac ttcggcagaa attcggaata ttgtcactcg
    48781 cctgttagaa aattggggtg caacctgtat cacacccgat gaaagattaa ttagtcaaga
    48841 ttatgatatc tttttaacgg ataatccgtc taatcttact gcctctggct tgcttttaag
    48901 cgatgatgag tctggcgtac gagaaattgg gcctggtcaa ttgtgcgtca acttcaatat
    48961 gagcaacgct atgcaggaag cggtcttaca attaattgaa gtgcaactgg cgcaggaaga
    49021 ggtgacagaa tcgcctctgg gcggagatga aaatgcgcaa ctccatgcca gcggctatta
    49081 tgcgctcttt gtagacacag taccggatga tgttaagagg ctgtatactg aagcagcaac
    49141 cagtgacttt gctgcgttag cccaaacggc tcatcgtctt aaaggcgtat ttgccatgct
    49201 aaatctggta cccggcaagc agttatgtga aacgctggaa catctgattc gtgagaagga
    49261 tgttccaaga atagaaaaat acatcagcga cattgacagt tatgtcaaga gcttgctgta
    49321 gcaaggtagc ctattacatg aacaatatga acgtaattat tgccgatgac catccgatag
    49381 tcttgttcgg tattcgcaaa tcacttgagc aaattgagtg ggtgaatgtt gtcggcgaat
    49441 ttgaagactc tacagcactg atcaacaacc tgccgaaact ggatgcgcat gtgttgatta
    49501 ccgatctctc catgcctggc gataagtacg gcgatggcat taccttaatc aagtacatca
    49561 agcgccattt cccaagcctg tcgatcattg ttctgactat gaacaacaac ccggcgattc
    49621 ttagtgcggt attggatctg gatatcgaag ggatcgtgct gaaacaaggt gcaccgaccg
    49681 atctgccgaa agctctcgcc gcgctgcaga aagggaagaa atttaccccg gaaagcgttt
    49741 ctcgcctgtt ggaaaaaatc agtgctggtg gttacggtga caagcgtctc tcgccaaaag
    49801 agagtgaagt tctgcgcctg tttgcggaag gcctcctagt gaccgagatc gctaaaaagc
    49861 tgaaccgcag tattaaaacc atcagtagcc agaagaaatc tgcgatgatg aagctgggcg
    49921 tcgagaacga tatcgccctg ctgaattatc tctcttctgt gaccttaagt ccggcagata
    49981 aagactaatc atctgtaggc cagataagac gcgttagtgt cttatctggc atttgcaccg
    50041 attgccggat gcggcgtaaa cgccttatcc ggcctacgat tcccattatt tcaacagatt
    50101 acattaaagt aggccagata agacgcgtca gtgtcttatc tggcatttgc actgaatgcc
    50161 ggatgcggcg taaacgcctt atccgtccta cgaatcccgc gatttcctga ccctctcggc
    50221 atataccgtc agcgtctttt ttatcacatc cagcgttacc ggcttcgaga ggcagctgtc
    50281 cataccggac tccagacacc gctgcttctc ttcagccaac gcattagcag ttactccgat
    50341 taccggcaac gtcagtccca actgacgaat gcgttgcgtc aagcggtaac catccatatt
    50401 tggcatgttg acgtcgctaa gcacgatatc aatatgattc ttactaagta cattaagcgc
    50461 atcgacgcca tcattcgcgg ttttacattg atagcccaac gatcccaact gatctgccag
    50521 cagacgtcgg ttaatcggat gatcatccac gaccagaatc atcatatcgt cgttatcgct
    50581 gaccgctttg tccgtcgacg gcagagcgtt agcaggatcg tcgctctcca tctcgatcaa
    50641 ataaatacgc gccaacaatg ccggtagctc atgcggagca gccacactgt gtacccactc
    50701 ccctggcgct ttctccagcg gaataccaat atggcgacga cagaaggtca ctaccgctct
    50761 gccctgccat tttttactca ctacctcgtc agtgattaac acatcttcgg gagtcggttc
    50821 ctgcccttcg tatgttgtaa cgacgatgcc gctgcgctgc aaactggttt ccaggaactg
    50881 acagagcgac gcattgcgga ccgccagcca gcagcgttta ccactcaacc cttccacgcc
    50941 ttttttctgc gggtactgag cgccgtacaa cggaatacgc acggtaaact ggctgcccat
    51001 tcccggttct gaatctaccg agatatcgcc gtccatcatg ctgatcagtt tttcacaaat
    51061 cgccagaccc agaccggtcc cctggaagtt tcgctgtacg cccgttccaa cctggaagaa
    51121 gggatcaaac aagcgcacca cttctttcgc cggaatcccc acgccggtat cgcgaacacg
    51181 gatagagaga taatcgccat cagcgcgaac atgcaaaact atacagccgg tatcggtgaa
    51241 ttttatggcg ttactcaaca ggttggagat gacctgctgt aaacgcatcg ggtcgccatt
    51301 taaggccact ggcacatccg gttcaataaa gcagtacaag cctaactgct tgcgtaccac
    51361 cagcggtaaa tagttggcgg tgatgtggtt catcacttca cgcggtgaaa actcacgcgg
    51421 ttcgatcttc aactgttccg attcaatctt cgagaaatcg agaatatcgc tgataatttt
    51481 caacaacagg ctggaagagt tgttcattgc cgtcaccaga cgatcgacgc ctttcggtaa
    51541 ctctttggtt tgcaacagat ccaggttacc gataatgcca tacagcggcg ttcgcagctc
    51601 atgactgacg gtggcaagga acatcgattt tgactggctc gcctgttccg ctgcttgtgc
    51661 catctcctgc aacgactctt ccatcttcac gcgcgaagaa acatccacca gcacacaaat
    51721 ggccacgttt tcattacgat agcgcgaatg gacgaagctg atttgcagat tggtattgtt
    51781 gctggtcagg acatcaacaa aattgacctg ctgcccacag ataatttgcg tcagtcgttg
    51841 gcggtcctca tgcgtaagca tattgagata ggtatgcgcc agttcgttac ttaaaatatt
    51901 gacgccatca gcggtacgca aaatgcagat acccactggc gcggaggcga caatcttgcg
    51961 attgaactgc tcatgttctt ccagtcgcag ggcgtcgctt tccgccggaa tgaaaatacg
    52021 tcgctcgtac atccgcgcga gagtaaacaa tgcagctccg gcaagcacat tcagcaaaat
    52081 tgcgttaagg atcaacatgc gaatgcgttc cagcacctta tcaaccggca ccgaatacac
    52141 gatgcttagc gatgagggtg gcagattttt cttcagcacc agctcccgga acccttccgt
    52201 atagccaaac caggagcgtt cctgcatcca gcgaggatcg cccttaattt tactttctgg
    52261 tccggtaagc gaaatcaggg tatgaccatt ttcatcaaga atggtaaccc ccatcggcaa
    52321 cgtacccggt aagaaaaagt tctccatccg gatggtctgc tcgacaccca aaagcgcctg
    52381 caaccggttc gccagataaa ctggcgtcaa cgcgtaaaaa tacccgacgc cagggcgcgg
    52441 accttcgctg atccagtaga ggttactgcc gctatcatct tgtggtgcat ttcgatattt
    52501 attgatgcgt tcatgcaacg ctttcaacgc ggtatcgcgt tccactggca tatcacgcag
    52561 accgaaattg gccatgcaga ggttatcact gccgattaaa aatacccggt tgagatcgta
    52621 agccgcagaa aaattatcgc gccagtagcg cataaaccac gccaatgact ccagagaacc
    52681 tcgccaggtg ttactcattg cggaacaatc ggagtcggca aacagcggtt caaacgcagg
    52741 cacatccgcc tgcgtttctc gtccacgcgg ggaaagcaca ccgttttctg ccgataagcg
    52801 attttcggcg atgtacttaa gctctttcat cacatcagag gtgcgttgaa taaagcgctg
    52861 agcctgatcg gaactcagat taaattcctg acgaatttcc gattctcgct gatgtaacgc
    52921 attaacgatg taaaaaacgg atgaaaaagc aatcaacagc cagagcacta acgccaatgc
    52981 tctgaacatg tagcgcgagg ctttcagggt tgtacgaaaa gaagcaaggt atttcaaagg
    53041 ggcgaagctc cgcctcaggt gaccgatgga gtgtggttaa ggtagcggta aaagcgtgtt
    53101 accgcaatgt tctgtcacca ttgcaataat gcgtatcgct tagaaaccat tgccttaacc
    53161 aggaaagccg gagtagatgg acagttctca gtacgaaaaa taagaaatga ccaatctgcc
    53221 gacgcttgtt aatggttata tcctggcgtc ggcagatcgc ccagcagtct tatctgaaaa
    53281 aagagagagc caggttgtgt gaagggatgg atcaccagtc aaaacttttc accagcgtca
    53341 gctcgccagc attacgcatc ggtacaataa atgtttcctg tttctcattg accgatcctt
    53401 catcggtgat cagcgtcagt tgggcggtcg tcaattccgt ttcgctgcgc ccaccatagt
    53461 agttgatata cacctgatag cgcccgtgaa ctggtgctgg catggcgaaa atctcgggtc
    53521 cgtaccccgt cgtgacatcc atatccagtg caccactgtt tttcagcacg gtgttaccgt
    53581 accaggcgtg ttcgccatcg ggcgtgacaa catggagatc gagatcggta ttgtccatat
    53641 cccacgagag aaccagccgt aaacgtgcac gaatagttcc tgtacccggc gttgagtaaa
    53701 actgcatttt ttgccggctt tgcccatccg ggctgatgac ctgcacgctg ttgctgcctt
    53761 cagtgaaaat ataagggcgt gcaaatgaac catcggattc aattctctgc ggcatactgg
    53821 caccgttgac caccagccgg ccctgctgaa ctttacccgc cgcagcataa ttctttattt
    53881 tgccgctgat ctgcgctgag atattttgat catctgccat gttgacggac gacgccgggt
    53941 aattgatcga ttgtgaaaaa ctggcatctt cgccctctgc cggatgccag ccagagagcg
    54001 gtgcgtcaat ctcgacttcc tgcacacctt cactgtgagc gacaggcgaa agtgccacca
    54061 gtaacaacgg aagaaaaatc tttcgcatag cattagtcca ttaataattg tcgggtcagc
    54121 gtttcgatat agttctcatc aagcccggtg ggataaccat caaaggccaa gtgcagatac
    54181 tcatgggtta aatccagccg atcctgaagc gtaaagaagt tacgaataaa cagccgtttc
    54241 tgctgccgat cggtataggg gaaacctgag actaaacgac agaccgcaaa cacgtctggt
    54301 tcgttgtacc ccgtctcgcc ttgtaatata cgccgccact gcggcatttt tttcgccagc
    54361 caggctttcg ctttgggtaa taactggcag gttgagcgtg gagctcccca gcggctaagg
    54421 ctgttgtcgg gataagcaaa cgcgagtatc tggtcgtaac gctccccctg ccccgcctgg
    54481 gccgtggctt gccgccagga aagtgtccct tcggtagcgc gactgccatg ataatgaacc
    54541 ggatcgcctg catagatgag ctcctgcgtc caggcggtca tcgttcgcgc ccccgtggtc
    54601 gctggcgaag cagaaacacg ctgcgtggcg ctgctgccgg ggatggtcag gcaatcacct
    54661 tcccgattgg cgttttgctg taaaaaagta cgtatcgcca cagtcatggc tttcgctgcc
    54721 tcaggtggcg tacttttcgc ctcgcgatcc agaactctgg caacgtactc ttcacgatcg
    54781 agatgcgact gcaatttcag tttgcccttt tcgctcagta aggtcgtctc accgtggctg
    54841 acaaacgtaa tgtggttacc attagtaaac gtcacacgat agcgcccatt aagtacgctg
    54901 ggttttactg ccgtcgtact cttttccgct gtgattttct tcagcggata gcgggcaaac
    54961 agctccacct ctacacattg cccactggca acctgcgtcg gcactggcaa aacccgatta
    55021 agcaccgtcg cataacgggt tagtaccgtt ttgctggtgc cactgccagt aacccacagc
    55081 ggtgtaccat cggttaacca gccagcaaaa ccaccctgac ggatttcctg tttatcatcg
    55141 gcaaaccagc tccaggtttt cacccgtacc cgactaccga gcatggatga gacaccgatt
    55201 ttcgcctcgt ccagcaccac atccagtaag acttcctgag ctttgttttg cgcaggtaac
    55261 gtcgctaatg aatcaagcag cgatttaacc gttaccgagg tttctggctt cagcgttgta
    55321 agagacgcta accacgcagg tgcctgacgc tgttgccagt attgccccca cacatctgcg
    55381 ccaatatgca accgctgcgg cgcaaaatag agcccacagg aacgtaccag cgcggtatcg
    55441 cgcgttatgc tctccccagc ctgacaacaa taaacctctt ccggggagtt tccgcgacac
    55501 tggtagggct gctccggttg atgggtatcc tccagccagg cgtaaacata cagtttccac
    55561 aaactcccta acggcgtggt gagcgagtca ggtaatgctg agactttggt cacgcctgat
    55621 gacgacagct gatacagttg atcgtgctgc gcaccgcgca gcaccagttg taacggcgtc
    55681 tcctccgcaa gcgttggcag agtaaccaac gccaatagcc agacgattct gcgccagttc
    55741 acttatttca cctgcatccc ggtccattca ctccctgccg cgacactttg ctgcgcaggt
    55801 gcataggaac gcacataacg agcaggaggc agaacgaatt gccctttttg cgagaagcgc
    55861 agcaaatggc ggaaagtgac cgttcccgtc agttctttca ccggcaccat ataggccagt
    55921 tcgcccattt cattacgcgc tttttccagc aattgcccct gctgttccgc ggcgttgggt
    55981 ttattgactg aaatgcccca tgttgtgcgc tcaacgtcgg ctcccggcgg gagcggtact
    56041 tccacctgac catagcgcag aactgcatcc tgctcgcagg taagcgtaat ttcatcgaga
    56101 tacagcgcat cgctgtcaat ctcattgctg gtcaccggct gcagagtaaa gctcatctct
    56161 tcttcaccgg ggataagccg atacaactgg cgttcaacgg tcaccggaat gttactttgt
    56221 tgagccgttt ttgccggttc acgccagcgg acctgcacat tttgcggcga taattcgtca
    56281 ccaaaagaga gaatgtccgg cacaccctga ccaacccaac gccagtcttc gccccctcca
    56341 gttaacttat gtttagccca tgcgcccgca ggcgcaggca aaacaactgg aggcattgtc
    56401 gccatatatt tcgccagcca gttcatggcc agcgcgcgct caatagtgga ttgctcagcg
    56461 gtcaaaccat taaaatagcg gccgtctggg tagcgtcacc accgctattg agcagcacaa
    56521 ccgtgtgcgc cagcggctga ttgctgtttg ccgctttatc ccacgcatat tgctgaacct
    56581 gtggcatcac cgtggacttc aactgcgctt ttttcagcaa cgtcatggtt aagacgtttg
    56641 ccaccgcatc tgccagtgga gattccggtg tatcgaggat caggctgtca ttgatatcgc
    56701 ggatgtcttc ctcagagaaa tcttcatctt tagttccgcg ccgggcgata gcttcatccc
    56761 cccctttcaa caacgttttg cacggcagat tcatctcctg tgcccacgcc agcaccagcg
    56821 cccgatgcaa taacggcatg ttatccgcct gctcggcata actgtcgagc atatgctgcc
    56881 agtattccgg ttgttgcgtt acgccgagcg cctggctggc ctgccaatcg gcgtaccatg
    56941 cccatgccgt aaggaaggcg tcaccattgc catcttcacc ccaccaggta aagcgcgctc
    57001 cgggccccgc cagttgcatc agccgcagac ggttatcctg aatcatctga cgaatgtcgt
    57061 tagcggcggc actttgatga tcggcaagcg aacgccaggc gagactgagc gggatcagac
    57121 ggctaccggt gttgatcacc ccaccccacg gttcatcgac taacgcatca agattgttgc
    57181 ggaaaatctc ctgcggcgtt tcactacttt gtagccggat attactcgcc tgctcgggca
    57241 acatcagcgc gttctcgcca ccgccgagca tgacattttt ctgctgttca acgggccagc
    57301 tgttgtccac aaaagacagt tttgtgctaa tgctgtcctg cacttgccca ttttgttgca
    57361 gttctgcact taacaagcca gattgctgaa tattctgcgt cagcgaaata taattcgccc
    57421 ctttgtgcag cgtcagcgtc tggcgcatct cagcgcctgc aaatttagtc accagcgcca
    57481 ccggttcgtt atcctgctga ctgaagataa acagtcctgc cgccggtttg tcgcccacgc
    57541 gatacaccgt tggcatactc cacttcatgt agagattttt ttccgaacgc agataagcac
    57601 gcccctgccc gaccagcccg tcgccgttca tcccacgcgc ggtgatacgc cagcgggtta
    57661 acgaatcagg catcaggaac gtgaaatacg ctttgccttg tttatcggtt gtgagtgacg
    57721 gcatccatgc cgcggtatcc acctcttcac gccgtggacg ttcaggcatt tttactcgcc
    57781 gctcgctgcg gttagtcgcg ccaggcgcaa ccggctcgct ggagagcgcc tggtcatagc
    57841 tgataaacga cagactggag ctggtacgca cattgttacg ccccagcgga tagaaaaatt
    57901 tgccgatatt cggcgcgatt tctggttgca gcgcgtagat catttcatcg accacgccta
    57961 ccgttagctg cgcagaaaca ggtttacctt tcagcgacga ggttaattct gaagtggtca
    58021 acaaaaactg gccaccgagt tagagttttt ccagtatcga ttttccgatt cgtttggggg
    58081 taacccaccg ttatattcgt gcggtctta
//
LOCUS       DAJTNM010000012        56453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTNM010000012 DAJTNM010000000
VERSION     DAJTNM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.012"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..1017)
                     /gene="setA"
                     /locus_tag="OO172_000838"
     CDS             complement(<1..1017)
                     /gene="setA"
                     /locus_tag="OO172_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5511119.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGA"
     gene            complement(1119..1250)
                     /gene="sgrT"
                     /locus_tag="OO172_000839"
     CDS             complement(1119..1250)
                     /gene="sgrT"
                     /locus_tag="OO172_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5511120.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1339..2994
                     /gene="sgrR"
                     /locus_tag="OO172_000840"
     CDS             1339..2994
                     /gene="sgrR"
                     /locus_tag="OO172_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5511121.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3028..3127
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3158..4141
                     /gene="thiB"
                     /locus_tag="OO172_000841"
     CDS             3158..4141
                     /gene="thiB"
                     /locus_tag="OO172_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5511122.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPSGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4117..5727
                     /gene="thiP"
                     /locus_tag="OO172_000842"
     CDS             4117..5727
                     /gene="thiP"
                     /locus_tag="OO172_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5511123.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5711..6409
                     /gene="thiQ"
                     /locus_tag="OO172_000843"
     CDS             5711..6409
                     /gene="thiQ"
                     /locus_tag="OO172_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5511124.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(6523..7287)
                     /locus_tag="OO172_000844"
     CDS             complement(6523..7287)
                     /locus_tag="OO172_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5511125.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7373..8251)
                     /gene="araC"
                     /locus_tag="OO172_000845"
     CDS             complement(7373..8251)
                     /gene="araC"
                     /locus_tag="OO172_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5511126.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8590..10226
                     /gene="araB"
                     /locus_tag="OO172_000846"
                     /pseudo
     CDS             8590..10226
                     /gene="araB"
                     /locus_tag="OO172_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            10237..11739
                     /gene="araA"
                     /locus_tag="OO172_000847"
     CDS             10237..11739
                     /gene="araA"
                     /locus_tag="OO172_000847"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5511127.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            11939..12634
                     /gene="araD"
                     /locus_tag="OO172_000848"
     CDS             11939..12634
                     /gene="araD"
                     /locus_tag="OO172_000848"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5511128.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12709..15060
                     /gene="polB"
                     /locus_tag="OO172_000849"
     CDS             12709..15060
                     /gene="polB"
                     /locus_tag="OO172_000849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5511129.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHNPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15224..18130
                     /gene="rapA"
                     /locus_tag="OO172_000850"
     CDS             15224..18130
                     /gene="rapA"
                     /locus_tag="OO172_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5511130.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAILLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18142..18801
                     /gene="rluA"
                     /locus_tag="OO172_000851"
     CDS             18142..18801
                     /gene="rluA"
                     /locus_tag="OO172_000851"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5511131.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(18918..19733)
                     /gene="djlA"
                     /locus_tag="OO172_000852"
     CDS             complement(18918..19733)
                     /gene="djlA"
                     /locus_tag="OO172_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5511132.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            19988..22342
                     /gene="lptD"
                     /locus_tag="OO172_000853"
     CDS             19988..22342
                     /gene="lptD"
                     /locus_tag="OO172_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5511133.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22395..23681
                     /gene="surA"
                     /locus_tag="OO172_000854"
     CDS             22395..23681
                     /gene="surA"
                     /locus_tag="OO172_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5511134.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRANAYVKILSN"
     gene            23681..24670
                     /gene="pdxA"
                     /locus_tag="OO172_000855"
     CDS             23681..24670
                     /gene="pdxA"
                     /locus_tag="OO172_000855"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5511135.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24667..25488
                     /gene="rsmA"
                     /locus_tag="OO172_000856"
     CDS             24667..25488
                     /gene="rsmA"
                     /locus_tag="OO172_000856"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5511136.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25491..25868
                     /gene="apaG"
                     /locus_tag="OO172_000857"
     CDS             25491..25868
                     /gene="apaG"
                     /locus_tag="OO172_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5511137.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25875..26717
                     /gene="apaH"
                     /locus_tag="OO172_000858"
     CDS             25875..26717
                     /gene="apaH"
                     /locus_tag="OO172_000858"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5511138.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26795..27274)
                     /gene="folA"
                     /locus_tag="OO172_000859"
     CDS             complement(26795..27274)
                     /gene="folA"
                     /locus_tag="OO172_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5511139.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27465..29327)
                     /gene="kefC"
                     /locus_tag="OO172_000860"
     CDS             complement(27465..29327)
                     /gene="kefC"
                     /locus_tag="OO172_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5511140.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29320..29850)
                     /gene="kefF"
                     /locus_tag="OO172_000861"
     CDS             complement(29320..29850)
                     /gene="kefF"
                     /locus_tag="OO172_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5511141.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(29898..31292)
                     /locus_tag="OO172_000862"
     CDS             complement(29898..31292)
                     /locus_tag="OO172_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5511142.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(31351..31638)
                     /gene="fixX"
                     /locus_tag="OO172_000863"
     CDS             complement(31351..31638)
                     /gene="fixX"
                     /locus_tag="OO172_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5511143.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31635..32921)
                     /locus_tag="OO172_000864"
     CDS             complement(31635..32921)
                     /locus_tag="OO172_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5511144.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESSPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(32972..33913)
                     /locus_tag="OO172_000865"
     CDS             complement(32972..33913)
                     /locus_tag="OO172_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5511145.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(33928..34698)
                     /locus_tag="OO172_000866"
     CDS             complement(33928..34698)
                     /locus_tag="OO172_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5511146.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVNA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35169..36683
                     /gene="caiT"
                     /locus_tag="OO172_000867"
     CDS             35169..36683
                     /gene="caiT"
                     /locus_tag="OO172_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5511147.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFDIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36714..37856
                     /gene="caiA"
                     /locus_tag="OO172_000868"
     CDS             36714..37856
                     /gene="caiA"
                     /locus_tag="OO172_000868"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5511148.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            37985..39202
                     /gene="caiB"
                     /locus_tag="OO172_000869"
     CDS             37985..39202
                     /gene="caiB"
                     /locus_tag="OO172_000869"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5511149.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            39276..40829
                     /gene="caiC"
                     /locus_tag="OO172_000870"
     CDS             39276..40829
                     /gene="caiC"
                     /locus_tag="OO172_000870"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5511150.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            40938..41723
                     /gene="caiD"
                     /locus_tag="OO172_000871"
     CDS             40938..41723
                     /gene="caiD"
                     /locus_tag="OO172_000871"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5511151.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41729..42319
                     /gene="caiE"
                     /locus_tag="OO172_000872"
     CDS             41729..42319
                     /gene="caiE"
                     /locus_tag="OO172_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5511152.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42405..42800)
                     /gene="caiF"
                     /locus_tag="OO172_000873"
     CDS             complement(42405..42800)
                     /gene="caiF"
                     /locus_tag="OO172_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5511153.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(43062..46283)
                     /gene="carB"
                     /locus_tag="OO172_000874"
     CDS             complement(43062..46283)
                     /gene="carB"
                     /locus_tag="OO172_000874"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5511154.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(46301..47449)
                     /gene="carA"
                     /locus_tag="OO172_000875"
     CDS             complement(46301..47449)
                     /gene="carA"
                     /locus_tag="OO172_000875"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5511155.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(47904..48725)
                     /gene="dapB"
                     /locus_tag="OO172_000876"
     CDS             complement(47904..48725)
                     /gene="dapB"
                     /locus_tag="OO172_000876"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5511156.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(48892..49806)
                     /gene="rihC"
                     /locus_tag="OO172_000877"
     CDS             complement(48892..49806)
                     /gene="rihC"
                     /locus_tag="OO172_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5511157.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(49872..50822)
                     /gene="ispH"
                     /locus_tag="OO172_000878"
                     /gene_synonym="lytB"
     CDS             complement(49872..50822)
                     /gene="ispH"
                     /locus_tag="OO172_000878"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5511158.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(50824..51273)
                     /gene="fkpB"
                     /locus_tag="OO172_000879"
     CDS             complement(50824..51273)
                     /gene="fkpB"
                     /locus_tag="OO172_000879"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5511159.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(51360..51854)
                     /gene="lspA"
                     /locus_tag="OO172_000880"
     CDS             complement(51360..51854)
                     /gene="lspA"
                     /locus_tag="OO172_000880"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5511160.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(51854..54670)
                     /gene="ileS"
                     /locus_tag="OO172_000881"
     CDS             complement(51854..54670)
                     /gene="ileS"
                     /locus_tag="OO172_000881"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5511161.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(54713..55654)
                     /gene="ribF"
                     /locus_tag="OO172_000882"
     CDS             complement(54713..55654)
                     /gene="ribF"
                     /locus_tag="OO172_000882"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5511162.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(55662..55880)
                     /locus_tag="OO172_000883"
     CDS             complement(55662..55880)
                     /locus_tag="OO172_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5511163.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            55983..56246
                     /gene="rpsT"
                     /locus_tag="OO172_000884"
     CDS             55983..56246
                     /gene="rpsT"
                     /locus_tag="OO172_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5511164.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        13502 a        15471 c        14670 g        12810 t
ORIGIN      
        1 tgcccccgct cttccaggca ttaaatcctg aaaccatagc atcccaatac ccgcaacaat
       61 gccgataaat acagcgttaa aaagttgcag cgtcatcaac gccatacggc tatgaaagaa
      121 aatcaatcca gtgtaaaaca gcactcctgc caccactgca atgaccatca ttcgccgctt
      181 accataacgt ttgacatagt agccagccag aatcattgct ggtatttcca gtccagctgc
      241 cgtccccatc aggaaacccg ccagtttgtc tggcaatcct aaatcgctac tgatccacaa
      301 cggcatatca ataatgtaca tagtgttgca ggtccacatt aacgtcgagg cgacaaataa
      361 catccgtacg ttactatcct gccagtcacc ttgcattgat aaagcatttt ccgacggcag
      421 ttctacccgc gccacagacg gaagcataaa tgcaatcaat accagactga gtgtgaatat
      481 cccggcggca atcgaaaaca tcactgtaaa gccgtaattc aacgccagca taaaggccag
      541 cggtggaccg attacccatg ccagagaaag ctgcgcacgc atcaccgagc taaacatcac
      601 cacttctcgc gccgagttat ccgcgtattc ccgcgccaga gcaaataact gtggcattgc
      661 cgtattggcc agagatgcca gaagcacacc acaggtgata agcgtcagat aatgacgatt
      721 aaatgcaaac agtagcgcat tgccaatagc catcaaacag caaaatataa tcagttttcg
      781 ccgatcgccc tgactgtcag aacgttttgc caaccagagg cttacgccga tcccagcaat
      841 agcattcacc gtataaaaga gtccgatcca gaaaggttgc gcgccaacct cacgactcag
      901 aaataagctc aatgtaggag cctgtagcgc cccggccacc cccatcataa aagcgaccag
      961 cataaatgcc gcgtaaacac cgttcatacg gcgagccatc gtcattatcc agatcatacg
     1021 ttcccttttt agcgcggcga gaataaaaaa aaccagcagg tataatctgc tggcgggtga
     1081 ttttacacca atactcagtc acacatgatg caggcaagtc aactttcaga attgcggtca
     1141 tcccactgca tcagtccttc aagcatggtt aatcgttgtg ggacgcttaa ccaacgcaac
     1201 cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa actgacgcat ggggcatctc
     1261 cttgcttcat cgttaggaat tattgactta atatagggaa aataaaattg ctgtcttttg
     1321 cgcaggagtt ccccttttat gccatctgct cgtctgcaac aacagttcat ccgcctgtgg
     1381 caatgctgcg agggtaaatc gcaggacaca acgctcaacg aactggcagc gttattgagc
     1441 tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc aggatcgcgg ctggctgacg
     1501 tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga cattcctcta taccgggctg
     1561 gcgcttcagc aacagcgggc ggaagacctg ctggagcagg atcgtatcga ccaactagtg
     1621 cagttggttg gcgacaaagc gactgtgcgg caaatgctgg tttctcatct gggccgcagc
     1681 ttccgccagg ggcggcacat cctgcgcgtg ctctactatc gtccgttgcg taatctgcta
     1741 cctggcagcg cattgcgccg ttccgaaacc catattgccc ggcaaatctt cagttcgcta
     1801 acgcgcataa atgaggaaaa tggggaactg gaagcagaca tcgcccacca ctggcagcaa
     1861 atatcaccgc ttcactggcg tttctttttg cgtccaggag tccattttca tcatggtcgt
     1921 gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa tcaatacgct gccgctctat
     1981 tcgcatatta cggacattgt gtcgccgacg ccctggacgc tggatatcca tctcacgcag
     2041 ccggaccgct ggttgccgtt actgctgggg caagttccgg cgatgatcct gccgtgcgaa
     2101 tgggaaaccc tcagtaactt tgccagccat cctattggca ccggtccgta tgcggtgatt
     2161 cgcaacagca ccaatcaact gaaaattcag gcattcgatg acttcttcgg ttaccgggca
     2221 ttaatcgacg aagttaacgt ctgggttctg ccggaaattg ccgacgagcc agccggtgat
     2281 ctgatgctaa aaggtccaca gggcgaggaa aaagagattg aaagccgcct ggaggaaggt
     2341 tgctactatt tactgttcga cagccgcacc catcgcgggg cgaatcagca agtcagggac
     2401 tgggtaagct atgtgctttc tccaactaat ctggtctatt tcgctgagga acagtaccag
     2461 caactgtggt tcccggctta tggactgctc ccccgttggc accatgcccg caccataaag
     2521 agcgaaaaac cggctggcct ggaaagcctc accctgacct tttatcagga tcacagtgag
     2581 catcgggtga ttgccgggat catgcagcag attctggcaa gtcaccaggt cacgctggaa
     2641 atcaaagaga tcagctacga tcagtggcat gaaggagaga tcgagagcga tatctggctt
     2701 aatagcgcca actttacgct gccgctggat ttttcgctgt tcgcgcactt gtgcgaggtg
     2761 ccgctgctcc aacactgtat tccgatcgac tggcaagccg acgccgcccg ctggcgcaat
     2821 ggcgaaatga acctggcgaa ctggtgccag caactggtcg ccagtaaagc aatggtgccg
     2881 cttatccacc actggctgat cattcaggga caacgcagta tgcgcggcct gcgtatgaat
     2941 accctcggct ggttcgattt taaatcagcg tggtttgcgc caccggatcc atgattgctg
     3001 gtagataaca aaatcactac actaacgccg ttctcaacgg ggtgccacgc aaacgcgtgc
     3061 gctgagaaaa tacccgtcga acctgatccg gataacgccg gcgaagggat ttgaggctcc
     3121 ttctcaagtc ctttgccact cttttttgag gtgcaaagtg ttaaaaaaat gtcttcccct
     3181 gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt ctgactgttt atacctacga
     3241 ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa aaagcttttg aagccgactg
     3301 taactgcgaa ctgaaactgg tggcgctgga agatggcgtt tcgcttctca accgtctacg
     3361 gatggaaggc aaaaatagta aagccgatgt ggtgctgggg ctggataaca atctgttaga
     3421 cgccgccagt aaaaccggac tatttgccaa aagcggtgtg gcagcggatg ccgttaacgt
     3481 tcccagcggc tggaataatg acactttcgt cccgtttgat tacggctatt tcgccttcgt
     3541 ctatgacaag aacaaactga aaaacccgcc gcaaagcctg aaagaattgg ttgagagcga
     3601 tcagaactgg cgggtgattt atcaggatcc gcgtaccagc acgccggggc tgggtctgct
     3661 gttgtggatg caaaaagtct atggcgatga cgccccacaa gcctggcaaa aactggcgaa
     3721 gaaaaccgtc acggtcacca aaggctggag cgaagcctac ggcctgtttt taaaaggtga
     3781 aagcgatctg gtactgagtt acaccacctc tccggcttat cacattctcg aagagaaaaa
     3841 agataactac gccgccgcga actttagcga aggtcactat ctgcaggtgg aagtcgccgc
     3901 ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa ttcctccagt ttatggtttc
     3961 tccggctttt cagaatgcga tcccaaccgg taactggatg tatccggtgg caaatgtcac
     4021 gctgcctgcc gggtttgaac aattgaccaa accggccacc acgctggagt tcacaccagc
     4081 tgaagtggcg gcacaacgtc aggcatggat tagcgaatgg caacgcgccg tcagccgtta
     4141 attcccggct ggttaattcc aggtgtaagc gccgccacgc tggtggtagc ggttgcgctc
     4201 gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta actgggtggc agtctggcag
     4261 gacagctacc tgtggcatgt ggtgcgcttc tccttctggc aggcgtttct ctcggcgcta
     4321 ctctctgtcg tacccgcgat attcctcgcc cgcgccctct atcgcaggcg ctttccgggt
     4381 cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc tcccggtgct agttgctgtt
     4441 ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg catcgctctg ccaatcgctc
     4501 ggtctggagt ggaccttttc gccctacggc ctgcaaggca ttttgctggc gcacgtattt
     4561 tttaatctgc cgatggcgag ccgcttatta ctccaggcac tggaaaacat tcccggcgaa
     4621 caacgtcagc ttgccgccca gcttgggatg cgcggctggc attttttccg cttcgtcgaa
     4681 tggccgtggc tacggcgaca aatcccgccg gttgccgcgc ttatctttat gctctgtttc
     4741 gccagcttcg ccaccgtgct atcgctgggc ggcggtccgc aggcaaccac tatcgagctg
     4801 gctatttatc aggcgctgag ttacgactac gatcctgccc gcgcggcgat gctggcgctg
     4861 ctccagatgg tgtgttgcct tgggctggtg ctgttgagtc agcgactaag taaggccatt
     4921 gcgcccggca ccacgctgct gcaaggctgg cgcgatccgg acgatcgtct gcatagccgc
     4981 atttgcgaca cggtgttaat agtgctggcg ctgctgctgt tgctgccacc gttgctggcg
     5041 gtgatcgtcg atggggtaaa tcgccagttg ccggaagtgc tggcacaacc ggtgctgtgg
     5101 caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag gtgtattgtg cgtagtgctg
     5161 accatgatgc tgctatggag cagtcgcgaa ctgcgggcgc ggcagaaaat gctggcgggt
     5221 caggcgctgg agatgagcgg catgttgatc ctcgccatgc cggggattgt gctggctacc
     5281 ggcttctttt tactgctcaa caacactatc ggcctgccac aatctgctga cggcattgtg
     5341 attttcacca atgcgttaat ggcgatccct tatgcgctga aagtactgga aaacccgatg
     5401 cgcgatatca ccgcccgcta cagtatgttg tgtcagtcgc tgggcattga aggctggtcg
     5461 cgcttaaaag tggtcgagct gcgcgccctg aaacgtccac tggcgcaggc gctggctttt
     5521 gcctgcgtgc tgtcgattgg tgattttggc gtagtggcgt tgttcggtaa cgatgatttc
     5581 cgtaccctgc cgttttatct ctaccagcaa attggctcct atcgcagcca ggacggcgcg
     5641 gtcaccgcgt taattctgct actactctgt tttctgctgt ttaccgtgat tgaaaaacta
     5701 ccggggcgaa atgttaaaac tgactgatat cacctggctt tatcaccatt tgccgatgcg
     5761 ttttagctta acggtagaac gcggcgagca ggtggcaatc ctcgggccaa gcggcgcggg
     5821 taaaagtacc ctgctgaatt tgattgccgg ttttctgacg ccagccagcg gttcgctgac
     5881 tatcgatggc gttgatcaca ccacgacgcc gccgtcacgc cgtccggtgt cgatgctgtt
     5941 tcaggagaac aacctgttca gccacctgac ggtcgcgcag aacatcgggc tggggctaaa
     6001 tccggggttg aaactgaacg cggcacagca ggagaaaatg cacgcgattg cccgtcagat
     6061 ggggattgat aatttaatgg cgcggttacc gggcgagctt tccggcggtc agcgacagcg
     6121 cgtggcgtta gcgcgttgtc tggtgcgcga acagccgatt ttattgctcg atgaaccgtt
     6181 ctctgcgctc gatccggcgt tacgtcagga gatgctgacg ctggtgagca cgagctgcca
     6241 gcagcaaaaa atgacgctat tgatggtgtc gcacagtgtg gaagatgcgg cgcggatcgc
     6301 cacacgctcg gtggtggtcg ccgacgggcg catcgcctgg cagggtaaga ctgaagaatt
     6361 gttgagcggt aaggcgagtg cttcggcaat attggggatt acgggttagt atgcactgtc
     6421 agatgcggcg tgaacacctt atccgaccta cggacagtgc gttttgtagg cctgataaga
     6481 cgcggtcagc gtcgcatgag gcaacccgca caagacaatc ccctaaaccc caaccacttt
     6541 acgcaaaata tcgatataca ccggcatcaa cgggtggcga attaacacca ccagcgccac
     6601 cacgccgatg gcagaaatca acggcgtcag ccacaacaaa cggccgcgcg acaaataatg
     6661 acttaaacga tcggttgctt taccgctgcg ccataaccgc cagcacagcc agccaccaac
     6721 ccacaaaaat accgccgtcg ccagcagcaa ccatttaaac tcgccgctct gcattccggc
     6781 tggaatatcg atcgccgcgc ccgccagaat cccaggcagg aagtaaaacg gtggccacag
     6841 caggcagccg ataatattcg gcgtaatgaa ttttgccacc ggcagatcca gcattcccgc
     6901 caccattggc accagcggac gcgtcgggcc aacaaagcga ccgaccagaa tggtgaacat
     6961 gctgtgttga tgcaacgcat gttcagtttt atcgagtaat gctttgtttt tcttcagaaa
     7021 tgaccagcga tgcaacggct ttttaaaacg ccagcccagc cagaaggaga tccagtcgcc
     7081 cagcagacaa ccgacaatcc ctgccagcca agcgtgccag aaacttaact cgccgctgcc
     7141 aatcagcgct cccagccccg ccatcagcac cgtaccgggt agaatcaaac cgaccagcgc
     7201 cagcgactcc agaaaggcca ccaacaccac cgccatcaat gaatacacgg tagattgggt
     7261 aataaagtgt tccagcaatg cttgcataat gtgcctgtca aatggacgaa gcagggattc
     7321 tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca
     7381 acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg
     7441 ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc
     7501 gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac
     7561 gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca
     7621 gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg
     7681 ccaggtgatc gctgatgtac tgacaagcct cgcgtacacg attatccatc ggtggatgga
     7741 gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca
     7801 gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc
     7861 tgaaatacgg ctggtgcgct tcatccgggc gaaagaaacc cgtattggca aatattgacg
     7921 gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc
     7981 attcgcgagc ctccggatga caaccgtagt gatgaatctc tcctggcggg aacagcaaaa
     8041 tatcacccgg tcggcagaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa
     8101 tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat
     8161 aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg
     8221 gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag
     8281 aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct
     8341 cgctaaccca accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag
     8401 ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt
     8461 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc
     8521 ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt ttttggatgg
     8581 agtgaaacga tggcgattgc aattggcctc gattttggca gtgattctgt gcgagctttg
     8641 gcggtggact gcgccagcgg tgaagagatc gccaccagcg tagagtggta tccccgttgg
     8701 caaaaagggc aattttgtga tgccccgaat aaccagttcc gtcatcatcc gcgtgactac
     8761 attgagtcaa tggaagcggc actgaaaacc gtgcttgcag agcttagcgt cgaacagcgc
     8821 gcagctgtgg tcgggattgg cgttgacagt accggctcga cgcccgcacc gattgatgcc
     8881 gacggtaacg tgctggcgct gcgcccggag tttgccgaaa acccgaacgc gatgttcgta
     8941 ttgtggaaag accacactgc ggttgaagaa gcggaagaga ttacccgttt gtgccacgcg
     9001 ccgggcaacg ttgactactc ccgctatatt ggcggtattt attccagcga atggttctgg
     9061 gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg cgcaatctgc cgcatcgtgg
     9121 attgagctgt gcgactgggt gccagctctg ctttccggta ccacccgccc gcaggatatt
     9181 cgtcgcggac gttgcagcgc cgggcataaa tctctgtggc acgaaagctg gggcggcttg
     9241 ccgccagcca gtttctttga tgagctggac ccgatcctca atcgccattt gccttccccg
     9301 ctgttcactg acacctggac tgccgatatt ccggtgggca ccttatgccc ggaatgggcg
     9361 cagttggcgc aggcgcacag cctaacgcac tggtaaaagt tatcggtact tccacctgcg
     9421 acattctgat tgccgacaaa cagagcgttg gcgagcgggc agttaaaggt atttgcggtc
     9481 aggttgatgg cagcgtggtg cctggattta tcggtctgga agcaggccaa tcggcgtttg
     9541 gtgatatcta cgcctggttt ggtcgcgtac tcggctggcc gctggaacag cttgccgccc
     9601 agcatgcgga actgaaagcg caaatcaacg ccagccagaa acaactgctt ccggcgctga
     9661 ccgaagcatg ggccaaaaat ccgtctctgg atcacctgcc ggtggtgctc gactggttta
     9721 acggtcgtcg cacgccaaac gctaaccaac gcctgaaagg ggtgattacc gatcttaacc
     9781 tcgctaccga cactccgctg ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac
     9841 gcgcaatcat ggagtgcttt accgatcagg ggatcgccgt caataacgtg atggcgctgg
     9901 gcggcatcgc gcggaaaaac caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc
     9961 cgctgcaaat tgttgcctct gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg
    10021 tcgccgcgaa agtgcacgca gacatcccat cagcccagca aaaaatggcc agtgcggtag
    10081 agaaaaccct gcaaccgcgc agcgaacagg cacaacgctt tgaacagctt tatcgccgct
    10141 atcagcaatg ggcgatgagc gccgaacaac actatcttcc aacttccgcc ccggcacagg
    10201 ctgcccaggc cgttgcgact ctataaggac acgataatga cgatttttga taattatgaa
    10261 gtgtggtttg tcattggcag ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc
    10321 caacatgccg agcacgttgt taatgcgctg aatacggaag cgaaactgcc ctgcaaactg
    10381 gtgttgaaac cgctgggcac cacgccggat gaaatcaccg ctatttgccg cgacgcgaat
    10441 tacgacgatc gttgcgctgg tctggtggtg tggctgcaca ccttctcccc ggccaaaatg
    10501 tggatcaacg gcctgaccat gctcaacaaa ccgttgctgc aattccacac ccagttcaac
    10561 gcggcgctgc cgtgggacag tatcgatatg gactttatga acctgaacca gactgcacat
    10621 ggcggtcgcg agttcggctt cattggcgcg cgtatgcgtc agcaacatgc cgtcgttacc
    10681 ggtcactggc aggataaaca agcccatgag cgtatcggct cctggatgcg tcaggcggtc
    10741 tctaaacagg atacccgtca tctgaaagtc tgccgatttg gcgataacat gcgtgaagtg
    10801 gcggtcaccg atggcgataa agttgccgca cagatcaagt tcggtttctc cgtcaatacc
    10861 tgggcggttg gcgatctggt gcaggtggtg aactccatca gcgacggcga tgttaacgcg
    10921 ctggtcgatg agtacgaaag ctgctacacc atgacgcctg ccacacaaat ccacggcgaa
    10981 aaacgacaga acgtgctgga agcggcgcgt attgagctgg ggatgaagcg tttcctggaa
    11041 caaggtggct tccacgcgtt caccaccacc tttgaagatt tgcacggtct gaaacagctt
    11101 ccaggtctgc ccgtacagcg tctgatgcag cagggttacg gctttgcggg cgaaggcgac
    11161 tggaaaaccg ccgccctgct tcgcatcatg aaggtgatgt caaccggtct gcagggcggc
    11221 acctccttta tggaggacta cacctatcac ttcgagaaag gtaatgacct ggtgctcggc
    11281 tcccatatgc tggaagtctg cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt
    11341 cagcatctcg gtattggtgg taaggacgat cctgcccgac tgatcttcaa tacccaaacc
    11401 ggtccagcga ttgtcgccag cctgattgat ctcggcgatc gttaccgtct gctggttaac
    11461 tgtatcgaca cggtgaaaac accgcactcc ctgccgaaac tgccggtggc taatgcactg
    11521 tggaaagcgc aaccggatct gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg
    11581 caccataccg tcttcagcca tgcgctgaac ctcaacgata tgcgccagtt cgccgagatg
    11641 cacgacattg aaatcacggt gattgataac gacacccgcc tgccagcgtt taaagacgca
    11701 ctgcgctgga acgaagtgta ttacggattt cgtcgctaag tagccacatc aggtatgtaa
    11761 cgcctgatgc gacgctgact cgtcttatct ggcctacacg ctgcgatttt gtaggccgga
    11821 taagcaaagc gcatccggca ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct
    11881 acgcgctgcg attttgtaga ccggataagc gaagcgcatc cggcacgaag gagtcaacat
    11941 gttagaagat ctcaaacgcc aggtattaga agccaacctg gcgctgccaa aacacaacct
    12001 ggtcacgctc acatggggca acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat
    12061 caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac gatatggtcg tggttagcat
    12121 cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc tccgacacgc caactcaccg
    12181 gctgctctat caggcattcc cctccattgg cggcattgtg catacgcact cgcgccacgc
    12241 caccatctgg gcgcaggcgg gtcagtcgat tccagcaacc ggcaccaccc acgccgacta
    12301 tttctacggc accattccct gcacccgcaa aatgaccgac gcagaaatca acggcgaata
    12361 tgagtgggaa accggtaacg tcatcgtaga aacctttgaa aaacagggta tcgatgcagc
    12421 gcaaatgccc ggcgttctgg tccattccca cggcccgttt gcatggggca aaaatgccga
    12481 agatgcggtg cataacgcca tcgtgctgga agaggtcgct tatatgggga tattctgccg
    12541 tcagttagcg ccgcagttac cggatatgca gcaaacgctg ctggataaac actatctgcg
    12601 taagcatggc gcgaaggcat attacgggca gtaatgactg tataaaacca cagccaatca
    12661 aacgaaacca ggctatactc aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca
    12721 ggttttatct taacccgaca ctggcgggac accccgcaag ggacagaagt ctccttctgg
    12781 ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt
    12841 attcacgccg atcaggttcc ccgcgctcag catattttgc agggtgaaca aggctttcgt
    12901 ctgacaccgc tggcgttaaa ggattttcac cgccagccgg tgtatggcct ttactgtcgc
    12961 gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg aaggtggcgt taccgtctac
    13021 gaggccgatg tgcgcccgcc agaacgctat ctgatggagc ggtttatcac ctcaccggtg
    13081 tgggtcgagg gtgatatgca caatggcact atcgttaatg cccgtctgaa accgcatccc
    13141 gactatcgtc cgccgctcaa gtgggtttct atagatattg aaaccacccg ccacggtgag
    13201 ctgtactgca tcggcctgga aggctgcggg cagcgcatcg tttatatgct ggggccggag
    13261 aatggcgacg cctcctcgct tgatttcgaa ctggaatacg tcgccagccg cccgctgctg
    13321 ctggaaaaac tcaacgcctg gtttgcaaac cacaatcccg atgtgatcat cggctggaac
    13381 gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg agcgttaccg tcttccgctg
    13441 cgtcttgggc gcgataatag cgagctggag tggcgcgagc acggctttaa aaacggcgtc
    13501 ttttttgccc aggctaaagg tcggctaatt atcgacggta tcgaggcgct gaaatccgcg
    13561 ttctggaatt tctcttcatt ctcgctggaa actgtcgctc aggagctatt aggcgaagga
    13621 aaatctatcg ataacccgtg ggttcgaatg gacgaaattg accgccgttt cgccgaagat
    13681 aaacctgcgc tggcaactta taacctgaaa gattgcgagc tggtgacgca gatcttccac
    13741 aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg tgaacggcct gccggtggat
    13801 cggcacggcg gttcggtggc agcgtttggt catctctatt ttccgcgaat gcatcgcgct
    13861 ggttatgtcg cgcctaatct cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg
    13921 atggattcac ggccagggct ttatgattca gtgctggtgc tggactataa aagcctgtac
    13981 ccgtcgatca tccgcacctt tctgattgat cccgtcgggc tggttgaagg catggcgcaa
    14041 cctgatccag agcacagtac cgaaggtttt ctcgatgcct ggttctcacg ggagaaacat
    14101 tgcctgccgg agattgtgac taacatctgg cacgggcgcg atgaagccaa acgtcagggt
    14161 aataagccgc tttcgcaggc gctgaagatc attatgaatg ccttttatgg cgtgctcggc
    14221 accaccgcct gccgcttctt cgatccgcgc ctggcatcgt cgatcaccat gcgtggtcat
    14281 cagatcatgc ggcagaccaa agcattgatt gaagcgcagg gctacgacgt tatctacgga
    14341 gataccgact cgacttttgt ctggctaaaa ggtgcacatt cggaagaaga ggcggcgaaa
    14401 atcggtcgtg cactggtgca gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa
    14461 cggctgacca gcgcattaga actggagtat gaaactcatt tctgccgttt tcttatgcca
    14521 accattcgcg gagctgatac cggcagtaaa aagcgctacg ccgggctgat tcaggagggc
    14581 gacaagcagc ggatggtgtt taaagggctg gaaaccgtgc gcaccgactg gacgccgctg
    14641 gcccagcagt ttcagcagga gctgtacctg cgtatcttcc gcaacgagcc atatcaggag
    14701 tacgtccgcc aaaccatcga caaactgatg gcgggtgaac tggatgcgcg gctggtttac
    14761 cgtaaacgcc ttcgccgtcc gctgagcgaa tatcagcgta atgttccgcc tcatgtacgc
    14821 gccgctcgcc ttgccgatga agaaaatcaa aagcgcggtc gccccttgca atatcagaat
    14881 cgcggcacca ttaagtacgt atggaccacc aacggcccgg agccgctgga ctaccaacgt
    14941 tcaccactgg attacgaaca ctatctgacc cgccagctac aacccgtggc ggagggaata
    15001 ctccctttta ttgaggataa ttttgctaca cttatgaccg ggcaacttgg gctattttga
    15061 gcaaaaaaag agttcgccag ataccatttt gatgcgtgac gaatgctttg ccatccagta
    15121 ccatagcgcc ctttccattc ctggacctga ataacaccac tacctcataa gcacggtagc
    15181 gggtggttat tgcctgcaat taaagatata gagccgaaca catatgcctt ttacacttgg
    15241 tcaacgctgg atcagcgata cagaaagcga attgggactt ggaaccgttg tcgcggtgga
    15301 tgcgcgaact gtcactttac ttttcccatc tactggtgaa aaccgtctgt acgcacgcag
    15361 tgattccccc gtgacccgcg tgatgttcaa ccctggtgat accattacca gccatgacgg
    15421 ctggcagatg caagtcgaag aagtaaaaga agaaaatggc ttgctgacct atatcggtac
    15481 tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa gttttccttg atagcaaact
    15541 ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag attgaccgta tggaccgctt
    15601 tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg
    15661 cctgcgcggt cagcgtacca gcctgatccc gcatcagctc aacatcgctc atgatgttgg
    15721 tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg ggtttaggga aaaccattga
    15781 agccgggatg atcctgcatc agcaactgct ctctggcgct gctgaacgtg tgctgattat
    15841 cgtcccggaa accttacagc atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg
    15901 ctttgctctg tttgatgatg agcgttatgc cgaagctcag cacgatgctt acaacccgtt
    15961 tgacaccgaa cagctggtga tttgctcgct ggattttgcc cgtcgtagca aacagcgcct
    16021 ggaacatctc tgtgaagccg aatgggacct gctggtggtc gatgaagcgc atcacctggt
    16081 gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt gaacaactgg cagagcacgt
    16141 gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg gggatggaaa gccacttcgc
    16201 ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca
    16261 gaaaaattat cgtccggttg cggacgccgt tgccattctg ctggcaggta acaaactgag
    16321 caatgacgaa ctgaacatgc tcggcgagat gatcggcgag caggatatcg agccgctgtt
    16381 gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat
    16441 gctgatggat cgccacggca ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa
    16501 aggattcccg aaacgcgagc tgcacaccat taagctgccg ctaccgacgc agtatcagac
    16561 ggctattaaa gtctccggca ttatgggcgc acgtaaaagt gcggaagatc gtgctcgcga
    16621 tatgctctac ccggagcgta tttatcagga atttgaaggt gataacgcca cctggtggaa
    16681 cttcgatccg cgcgttgagt ggctgatggg ctacctgacc agccatcgct ctcagaaagt
    16741 gctggtgatc tgcgccaaag ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg
    16801 tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg attatcgaac gtgaccgcgc
    16861 tgccgcctgg tttgccgaag aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg
    16921 ttctgaagga cgtaactttc agttcgccag ccacatggtg atgtttgacc tgccattcaa
    16981 cccggatcta ctggagcagc gtattggtcg tctggatcgt atcggccagg cgcacgatat
    17041 tcagatccat gtgccttatc tggagaaaac cgctcagtcg gtgctggtgc gctggtatca
    17101 cgaaggtctg gatgcatttg agcacacctg cccgaccgga cgcactattt acgatagcgt
    17161 atacaacgat ctgattaact atctggcttc accggatcaa accgaaggct ttgacgatct
    17221 gatcaaaagc tgccgcgagc aacatgaagc gctgaaagca cagctggaac agggtcgtga
    17281 ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat
    17341 tgaagagcag gatgacgata ccaacctgat cgccttcgcc atgaacctgt tcgatattat
    17401 cggtatcaat caggacgatc gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat
    17461 gctggtgccg gacttccctg gcctgtcgga agatggcatc accatcacct ttgatcgtga
    17521 agtggcgctg gcgcgtgaag atgcacagtt tattacctgg gagcatccgc tgatccgcaa
    17581 cggtctggat ctgatccttt ctggcgatac cggtagcagc acgatttcac tgttaaaaaa
    17641 caaagcgttg ccggtaggta cgctgttggt ggaactgatt tatgtggttg aagcccaggc
    17701 tccgaagcag ttgcagctca accgcttcct gccaccgacg ccggtacgta tgctgctgga
    17761 taaaaacggc aacaacctgg cggcgcaggt agagtttgaa acctttaacc gccagcttaa
    17821 cgcggttaac cgtcacaccg gcagcaaact ggttaacacc gtgcagcagg atgttcacgc
    17881 tatccttcaa ctgggtgaag cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc
    17941 gcgtaacgaa gccgacgaaa aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc
    18001 agtgaacccg aacattcgtg acgacgaact gaccgccatt gagagcaacc gtcagcaggt
    18061 aatggaaagc ctggatcagg caggttggcg tctggatgcc ctgcgtttga tcgttgtaac
    18121 gcatcagtaa cggagccgaa aatggggatg gaaaactaca atccaccgca ggaaccctgg
    18181 ttggttatcc tgtatcagga tgaccatatt atggtggtca acaagccgag cggtttgttg
    18241 tcagtgccgg gtcgtctgga agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat
    18301 tatccgcagg cagaatcggt gcatcgtctg gatatggcta ccagcggcgt gattgtagtg
    18361 gcgctgacca aagccgcgga gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa
    18421 aagcagtatg tggcccgcgt ctgggggcat ccatcccccg cagaaggtct ggtggatctg
    18481 ccgctgattt gcgactggcc aaaccgcccg aaacagaaag tctgttacga aacgggtaaa
    18541 cctgcgcaga cggaatatga agtggtggag tatgcggcgg ataacacggc aagagcggtg
    18601 ttaaaaccga ttaccgggcg ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat
    18661 ccgattctcg gcgatcgttt ttatgcatca ccagaagcga gagcgatggc accgcgtttg
    18721 ttgctacatg cagagatgtt gacgattacc catccggcgt atggcaatag tatgacgttt
    18781 aaagcgccag cggattttta atcgtcacgc tggcgcagtt cggtaggcgg ataaggcgtt
    18841 cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg acgcgggccg cgtcttatca
    18901 ggcctacagt ttaaaactta tttaaaccct ttctgctgct ttatcagctc atatgcctgc
    18961 tgaatttcct gcgctttctg cttcgccatc tccatcatct caggcggcaa acctttcgcc
    19021 accagcttat cgggatggtg ttcactcatc agcttacggt aggcacgttt gatggtggtc
    19081 gcatcatccg tcggcttcac gcccagcaca ttacaggcat cttccagcgt cgggccacgc
    19141 tgcgcttgct gccagttacc accgccagat tgctgctgat aaccgccgcc aaactgtgca
    19201 ccgccctgca tcatgcgcaa aaactggtca aactgggcgc gggagatccc taattcttca
    19261 gcaatgacat acagcaccgc ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc
    19321 tgaatctgga tctccagaaa catacgaatt aagtcaaaac gaccaaagca gacactgcga
    19381 aactggcgca tcttttcgcg cagcgggtaa ttatctgatt ttcccacccg gaacgcgttt
    19441 tgcgccgcag tacgggaagc gccatgaagg ttcattcggt ccatcaactg gctggcgata
    19501 tgaatatcag cctccgtgac gcgacctttg gatttggtta aatgccccat cacttcaaaa
    19561 gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc cattttacgg
    19621 ctacgggctt tatcaaacat atggccaatt aacaggccta acactacgcc ccaaaagccg
    19681 ccgcccatca gtaaggccac ggccacgcca atgatttttc cccaatactg catatattcc
    19741 ccaaatcgac acacggatat cagggctatc tcccacaata taaaggtgct tttaccgttt
    19801 tccggctgcg gtcaattgtg ggacatcgcc tataatttgc attatcatac ctgtcattca
    19861 atgccgtgcc taacaccact gacgctattc ggacaggatt aacactagcg tagagatgac
    19921 aagtacgtta gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa
    19981 tcaacgtatg aaaaaacgta tccccactct cctggccacc atgattgcca ccgcccttta
    20041 tagtcaacag ggactggcag ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta
    20101 tgaccgtcct ctggtacagg gcgataccaa tgacttaccc gtgactatca atgctgacca
    20161 cgcgaaaggg gactacccgg atgacgccgt gtttactggc agcgtggata tcatgcaggg
    20221 taacagccgt ctgcaggccg acgaagtgca gctccatcaa aaagaggcac caggacaacc
    20281 ggagccggta cgtaccgttg atgcgctcgg taatgtccat tacgacgata accaggtgat
    20341 cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa gataccaacg tctgggaagg
    20401 tgattaccag atggtgggtc gccagggtcg cggtaaagcg gacctgatga aacaacgtgg
    20461 cgaaaaccgc tataccattc tggataacgg tagctttacc tcctgtctgc cgggttctga
    20521 cacctggagc gtggtaggta gcgaaattat tcatgaccgc gaagaacaag ttgcggagat
    20581 ctggaacgcc cgctttaagg tgggtccggt accgatcttt tatagcccct atttgcagtt
    20641 gccggtgggt gacaaacgtc gctctggttt cttgatcccg aacgccaagt acaccaccac
    20701 caactacttt gagttctacc tgccatatta ctggaacatc gcgccaaata tggatgccac
    20761 catcacgccg cattatatgc atcgtcgtgg caacatcatg tgggagaacg aattccgcta
    20821 cctctcccag gcgggcgctg gcttgatgga actggactat ctgccttcag ataaagtcta
    20881 tgaagatgaa cacccgaacg atgacagttc acgtcgttgg ttattctact ggaaccactc
    20941 cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca gcgatcctag
    21001 ctacttcaat gatttcgata acaagtacgg ttccagtact gacggctacg caacgcaaaa
    21061 attcagcgtt ggctatgcgg tgcaaaactt caatgccacc gtttcaacca agcagttcca
    21121 ggttttcagc gaacagaaca ccagtagcta ctcggcagag ccgcagttag acgttaatta
    21181 ctaccagaat gatgttggtc cgtttgatac gcgtatttac ggccaggcag tgcactttgt
    21241 taacaccaga gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt
    21301 gccgctctct aataactggg gcagcatcaa taccgaagcg aagtttctgg caacccatta
    21361 tcagcaaacc aatcttgact ggtataactc cagaaacacg accaagctgg acgaatccgt
    21421 taaccgcgta atgccgcaat tcaaagttga cggcaaaatg gtctttgaac gcgatatgga
    21481 aatgctggct ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc
    21541 gtatcgcgat cagagcgaca tctataacta cgactcgtct ctgctgcaat ctgactactc
    21601 tggcctgttc cgggaccgga cttacggcgg tcttgaccgt attgcctccg ctaaccaggt
    21661 gacgaccggt gtcacatctc gcatatatga tgatgctgcc gttgaacgtt ttaatatttc
    21721 cgttggtcaa atctactatt tcacggagtc tcgcactggc gatgacaaca taacatggga
    21781 gaatgacgac aaaacgggct cactggtgtg ggcaggcgac acctactggc gtatctccga
    21841 gcgttgggga ttgcgtggcg ggattcagta cgatacacgt ctggataacg tagcgaccag
    21901 taactccagc attgaatacc gtcgggatga agaccgtctg gtacagctga attaccatta
    21961 cgccagcccg gaatatattc aggctacgct gcctaagtac tattccactg ctgagcaata
    22021 taagaatggt atttcgcagg taggtgctgt cgccagcagg ccaattgccg atcgttggtc
    22081 cattgttggg gcctactact acgacaccaa tgcgaacaag caagccgact ctatgttagg
    22141 tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg
    22201 ttgggataac gataaacaac atgcggtata tgacaacgca atcggcttta acatcgaact
    22261 tcgcggcctg agctccaact acggtctggg tacgcaagag atgctgcgtt cgaacattct
    22321 gccgtatcaa aacactttgt gatttgttga tttaccacgt aatccgcagt gcggttaatt
    22381 gaaatggaaa aagtatgaag aactggaaaa cgctgcttct cggtatcgcc atgatcgcga
    22441 ataccagttt cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc aataacggcg
    22501 tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt aaaactgaac gctgcgcagg
    22561 caaggcagca gcttcctgat gacgcgacgc tgcgccacca aatcatggaa cgtttgatca
    22621 tggatcaaat catcctgcag atggggcaga aaatgggagt gaaaatctcc gatgagcagc
    22681 tggatcaggc gattgctaac atcgcgaaac agaacaacat gacgctggat cagatgcgca
    22741 gccgtctggc ttacgatggt ctgaactaca acacttatcg taaccagatc cgcaaagaga
    22801 tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg
    22861 aagtcgaatc cctggcgcag caggtgggta accaaaacga cgccagcact gagctgaacc
    22921 tgagccacat cctgatcccg ctgccggaaa acccgacctc tgatcaggtg aacgaagcgg
    22981 aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg
    23041 cgattgctca ttctgccgac cagcaggcgc tgaacggcgg ccagatgggg tggggccgta
    23101 ttcaggagtt gccggggatc ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg
    23161 ttggcccgat tcgttccggc gttggcttcc atattctgaa agttaacgac ctgcgcggcg
    23221 aaagcaaaaa tatctcggtg accgaagttc atgctcgcca tattctgctg aaaccgtcgc
    23281 cgatcatgac tgacgaacag gcccgcgtga aactggagca aattgctgct gatatcaaga
    23341 gtggtaaaac gacttttgct gccgcagcga aagagttctc gcaggatcca ggttctgcta
    23401 accagggcgg tgatctcggc tgggctacac cagatatttt cgatccagcc ttccgtgacg
    23461 ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca ttcggctggc
    23521 atttaatcga actgctggat acccgtaatg tcgataaaac cgacgctgcg cagaaagatc
    23581 gtgcataccg catgctgatg aaccgtaagt tctcggaaga agcagcaagc tggatgcagg
    23641 aacaacgtgc caacgcctac gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt
    23701 tgtgatcact cccggcgagc ccgccgggat tggcccggac ttagttgtcc agcttgcaca
    23761 gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc actctcctta ccgaccgggc
    23821 agcgatgctc ggtttgccgc tcaccctccg cccttattcc cccaactccc ctgcacaacc
    23881 gcaaactacg ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg tcactgcggg
    23941 gcagttagcg attgaaaatg ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg
    24001 ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa
    24061 cgacgcaagc attccgttta ccggtcatac cgagtttttc gaagagcgtt cgcaggcgaa
    24121 aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt
    24181 accgctgcgc gatatcgcag atgctatcac ccctgcactt ttgcacgaag tgattgctat
    24241 tttgcatcac gatttgcgta caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg
    24301 gctgaatccg cacgcgggcg aaggcggtca tatgggtacg gaagagatag acaccattat
    24361 tccggtgctc gacgagctgc gggcgcaggg aatgaaactc aacgggccgc tgcctgccga
    24421 taccctgttt cagccgaaat atctcgataa cgccgacgcc gtgctggcga tgtaccacga
    24481 tcagggtctt cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg
    24541 cctgcccttt attcgcacat cagtggacca cggcaccgcg cttgaactgg cgggacgtgg
    24601 cgaagccgat gtcggcagtt ttattacggc gcttaatctc gccatcaaaa tgattgttaa
    24661 cacccaatga ataatcgagt ccaccagggc cacttagccc gtaaacgctt cgggcaaaac
    24721 tttctcaacg atcagttcgt gatcgacagc attgtttctg ccattaaccc gcaaaagggc
    24781 caggcaatgg tcgaaatcgg ccccggtctt gcggcattga ccgagccggt cggcgaacgt
    24841 ctggaccagc tgacggtcat cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat
    24901 ccattcttag gcccgaaact gacgatttat cagcaggatg cgatgacctt taactttggc
    24961 gaactggccg agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc ttataacatc
    25021 tccacgccgt tgatgttcca tctgtttagc tatactgatg ccattgccga catgcacttt
    25081 atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt
    25141 cgattaagcg tcatggcgca atactattgc aacgtgatcc cggtactgga agttccgccg
    25201 tcagccttta caccaccacc caaagtggat tccgccgtcg tgcgcctggt ccctcatgca
    25261 acgatgcctc acccggttaa agatgttcgc gtgttgagcc gcatcaccac cgaagccttt
    25321 aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta
    25381 accggaatgg ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc
    25441 cagatggcga actatctggc ggagaacgcg cctttgcagg agagttaacg atgatcaatt
    25501 cgccccgagt gtgtattcag gttcaaagcg tctacattga ggcacaatct tcacctgata
    25561 atgaacgtta cgtttttgct tataccgtaa ccatacgcaa tctggggcga gcgccagtgc
    25621 agttgttggg gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg
    25681 gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg cgaagagtac cagtacacca
    25741 gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg tcactacgaa atgatcgatg
    25801 aaaatggcgt ccctttcagc atcgacattc ccgtattccg actcgccgtt cccacactca
    25861 ttcattaaaa gaatatggcg acatacctta ttggcgacgt tcatggttgt tacgatgaac
    25921 tgatcgcatt gctgcataaa gtagaattta cccccgggaa agataccctc tggctgacgg
    25981 gcgatctggt cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg aaatccttag
    26041 gcgacagcgt acgtctggtg ctgggcaatc acgatctgca tctgctggcg gtatttgccg
    26101 ggatcagccg caataaaccg aaagatcgcc tgacaccgct gctggaagcg ccggatgccg
    26161 acgagctgct taactggctg cgtcgccagc ctctgctgca aatcgacgaa gagaaaaagc
    26221 tggtgatggc ccacgcaggg atcacgccgc agtgggatct gcagaccgcc aaagagtgcg
    26281 cgcgcgatgt agaagcggtg ctgtcgagtg actcctatcc cttctttctt gacgctatgt
    26341 acggcgatat gccaaataac tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta
    26401 tcaccaacgc ctttacccgt atgcgttttt gcttcccgaa cggtcaactg gatatgtaca
    26461 gcaaagaatc gccggaagaa gcccctgccc cactgaaacc gtggtttgcg attcctggcc
    26521 ctgtcgctga agaatacagc atcgcctttg gtcactgggc atcgctggaa ggcaaaggta
    26581 cgccggaagg tatttacgcg ctggataccg gctgctgctg gggtgggtca ttaacctgcc
    26641 tgcgctggga agataaacag tattttgtcc agccgtcgaa ccggcataag gatttgggcg
    26701 aagcggcggc gtcttaaaca cagcctgata caggaaggcc ggataagacg cgaccggcgt
    26761 cgcatccggc actagccgta aattctatac aaacttaccg ccgctccaga atctcaaagc
    26821 aatagctgtg agagttctgc gcatcggcgt cgtggaattc gctgaatacc gattcccagt
    26881 catccggctc gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca
    26941 gatacagttt ttgcgctttt ggcaggaact gctcataaac gcgaccgccg ccaatcacca
    27001 tgatttctgg tacgtcacca cacgccgcaa tggcttcatc caccgatttc acccacgtta
    27061 cgcgatcgtc cgtacccggt tgactgctga ggataatatt tttgcgtcct ggcaacggac
    27121 gaccgattga ttcccaagta tggcggccca taatcacggg tttatttaag gtgttgcgtt
    27181 taaaccaggc gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc
    27241 gatctaccgc taacgccgca atcagactga tcattgagat ttcccgataa aaaaattgtc
    27301 gccactatac gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat
    27361 tttatattct gctggcgagt ccacgctctc ccccaggact cgccgcatta caatgaaaca
    27421 aaaacaaaca gttagctgta aagtgtgatt tacgtcactc tttattaaga tgagggtttc
    27481 gtttccggtt catccgccat gttgccggta tgtttacctt cttctgttcc ctgccagcca
    27541 tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtga tgatctcact taacatcgcg
    27601 ctggtgcgtt tatagaccgc cgcgcgggct ttggtgtcat tctcaaccat tgccatctct
    27661 tccaccatct gaatattaaa gcggcggaac acatcggcac gttctcgcgc ttcatacggc
    27721 cccagaccta aactttccag cgccagacgc ccggttttca gcgcaccttc aaaggtttca
    27781 cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt agtgatcgac atcgcgggcg
    27841 cgggcaataa tctgcaaatg cgggaaatgt tctttcacca tctccgtcag ttgcaggttg
    27901 gtttgcggat cgtcaatagc gttaatcagc acttccgctt tcgccgctcc ggcagattcc
    27961 agtaaatcca tccgcgtggc atcgccataa aacactttca taccaaattt acgcagcgtt
    28021 tcgatatggt ccggatcgtg atcgagcacc accattttca ccccgctgga gagcagtaaa
    28081 cgtccggtaa tctgtccaaa acgaccgaat ccggcgataa tcacgcgcgg ctgttcttca
    28141 tcgatctcat cggcttcacg cgcctcctcc gtagaagatt gctcaaggcg attgaggatc
    28201 accagcagaa tcggcgttgc tgccatcgac agcgccaccg ccagggtcag cgatttcgcc
    28261 cactccggct ccagcacatt cgccatctgc gccgcaccaa ataccacaaa ggcaaactca
    28321 ctgccctgcc ctaacaacac cgcaaaccag cgacgctgtt tatttggcac ctgcaacggt
    28381 cgggcaatca gccacagcat ggcgattttg atgatgagga aaccgagcag caaaatgaca
    28441 atgcgcaatg ggttttcaat cagcgtgcca aagtctatcg acatgccaac accgatgaaa
    28501 aacagcccca acagcaaacc tttaaacggt tcgatatcgc tttccaacgc atgacggtat
    28561 tccgagcttg ccagcagtac gcccgccaga aacgcgccca tcgccatcga caagccgacc
    28621 tcttccagca gcaaaccaaa gccaaacaca aggaataacg ccacggcact aaacacttcc
    28681 cgcaagccag agcgggcaac aaaacgcagc gccggacgcg taacatagcg ccccagcaat
    28741 accaccagta ccagcgcacc cgccactttt aacgccgaga gagcaaatgc gcccatcgtc
    28801 gtcgaggcac tgctcgccgc cagtagcggg atcatcgcca ccagcgggat tgccgcgata
    28861 tcctggaaca gcagcactgc aaaggcactg cggcccattt gcgtcaccat cagattgcgt
    28921 tcattcatcg cctgcatggc aatcgccgtt gatgagagcg ccagcgtcat accgatcaat
    28981 tccgcgacct gccagcgcag cccaagtaac atacagaaca gccccagcag gccaccgcaa
    29041 atcaccatct gcaatgcgcc accgccgaac actgccgcac gcagcttcca cagcctttgt
    29101 ggatcgagtt cgaggccgat aataaacagc atcagcacca ccccaatctc ggcaaagtgc
    29161 agaatagatt cggcatcggt caccagtcgc agcccccacg ggccaataat gcagccggcg
    29221 atcaggtagc caagtaccga tcccagacca agacgtaccg caatgggtac aatcagcgcc
    29281 gccgatccga ggtagatcag cgcctgaacc agcgtatgac tatccatgat gggcctcctg
    29341 ccattccagc agacgttgct tatagtggcg cgcctgccct tcgagggttt cgtcgtcaca
    29401 aataaaggtg cagtgcatgg caaacggtgg cagccagttc agcccgcagt agattgccgt
    29461 cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt caaaatggct
    29521 ttccccgccg ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc
    29581 gtgaccgtaa gcccagccgt gcgacaaaac tttatcgatc caaagtttaa gaagcggagg
    29641 aatgctgtac cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag agagcgcctc
    29701 ctgctcggcg gcaatatcga tattgaagtc aggatagagt tgataaagag agcgaatttc
    29761 gacgccttcc agcgtccttg cctgttcaag catccgttta ttcgcatggg aatgatgcgg
    29821 atacggatgc gcataaatga tgaggatcat agttattagc ccgcgtgttt acgcctttgt
    29881 tttcccacaa agtgtagtca tatattcacg cggcttatag tcaataatat tgattcagaa
    29941 aagagagaaa tctgaacaaa taatcccatt ctctgcccgc ggatcgtcat attgctggtc
    30001 tgcgccagtg acattcctcg agtctccggg gcaaacgcta cggaaatcaa caagccaaac
    30061 agcgagatac ccgcccccat tagcatcgtg ttactgatac cgtaattatt gataaatatc
    30121 ggcagtgccc aggtcgaaac aatggtgcca atacgactta aggacataat cacgcccacg
    30181 gcagaggcgc ggatatccgt cgggaagagt tcattaggat agagccactg caaattaccc
    30241 ggcccgccag agaaaaaggc atacaccgca aaggccatca ctaccagcca gatccccata
    30301 tccgggatta gccccaaaac cgccagcgcc agcgtcatca tggcaaagct gccaatcaac
    30361 aatggacgcc gtccggcagt gtttagccac agcatcggcg gaatacagcc gagcataaag
    30421 aacaggctaa tcaccacatt ccctagtgcc gcgtttttgc caacccccaa tcccaacgaa
    30481 ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag
    30541 atggcggcaa caaacagaac aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg
    30601 gtttgctgcg gctgctcttc atcgaaagcc accggttcgc caaacagttt tatcatcatc
    30661 tcttcgcact cttttactcg ccctttgcgt aataaccagc ggggagattc tggcagttcg
    30721 aatcgaccaa tcaaaatcaa caaacaaggg atcgccgcgc tacccagcat ccagcgccaa
    30781 ccaccttcca catcataaag ccagtagccg accagatcgg cgcaggtcgc gccgacatac
    30841 cacatcgcgg caataaagct gatggaaaac gcccgctgac gggtactgga gaactcggtg
    30901 atcatcgaag tggcgatggg ataatctgca ccgatgacaa tgccgatgag tacccgcatc
    30961 accaacagtt cgacggggga tgaaacacac atcgtcgcca ccgatatcac gccgatggcg
    31021 atgatatcaa tgaggaacat tttgcgccgt ccgactttat cggagatata gccaaacagc
    31081 gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg
    31141 tccagtttca gcgccggagt cagttgctcc agcgccacgc caatcattac cagtacataa
    31201 ccatccagaa acggaccacc gcttccccac agcaaaatgc ggcggtgaat agaggagaat
    31261 ttgagatcgt caaagtttct ggacggttgc atgggttatt cctgtttttt tgcaatgaga
    31321 gtcagtacgg cgttacgtat caaaccaaca tcagccgtaa cggaactcca caccaaaggt
    31381 gccgcgcggg tattcccact gttccagcgc cgaccccagc cccagaatgc gacaggtgcc
    31441 gcactccaga catccggcgt aatcgaagcg cacactgccg tcatcctgct tcttgtacag
    31501 acctgcgggg cacgctttca ccagcagctc cagcacctgt ttatcagcat cggccttcac
    31561 aacaatgtgc ggatgctctt catcgacatt gaatttattg acgcccagtt tgacgtccac
    31621 attgacggga gaagtcataa aacggtcact cctttcatgc catccttgat cagattgatg
    31681 aagcccactt tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg cgcactgccg
    31741 tcaatggtga acaggtcacg cgccacgccc accgccagtt ccgggtagcc gctaaacatg
    31801 cgtgggttat caaggaacgc cggtagtttc tggtacatgc gcatatcgcg cagcgggctg
    31861 ctctccagat gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc gcttttcatc
    31921 gctgaaagca cggtttttgc tgcagcttcc ccggcggcaa tcgccagatc cataccgcga
    31981 atggtaaaac cgaggttcat acacattccg gcggcatcac cggcaatcaa tacgccgtca
    32041 ccgaccagct ccggcagcat gttgatgcct gcttccggca ctacgtgagc ggaatattcc
    32101 accagcttgc cgcccgcgat cagcggtgca acggccggat gctgtttgaa atcttccagc
    32161 atttgcggca ccgatttttt cgcgtcatgc agatgatgca gaccacaaac cagccccagc
    32221 gacagggtat tttcattggt ataaaggaag ccgccgccca tcaggccatc ggtgggtgat
    32281 cccgcaaaca gacaagccgc gccctgatta ccctgcaact gaaaacggtc ttcgattacc
    32341 gacttcggta actcgatcag ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt
    32401 tttgccatcc ccaacttttc ggcaaggatg gagttcaccc catcagcaag gagcaccgtt
    32461 ttcgcttcaa tcacatcgcc atcggcttct acaccgacga ctttgccatc gcgctgtacg
    32521 aggttatcga cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc
    32581 aaccaggcat caaatttact gcgcaaaacg gagtaagaac gctgggatag cgaggtttcg
    32641 tcaccgttgc agtagtccat agtcatcgct gacttttccg tcataaacgc gagtttttca
    32701 tgggtgatca ggcgttctac gggggcggaa tcggcgaaac ctggaataat gtgttccaga
    32761 ctgtgggcat agagacgccc gccagtaacg ttcttggcac ccgcggaatt gccacgctcg
    32821 ataactaaca cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga accggcaagc
    32881 cctgcaccga cgatgatggc gtcaaagata tcttcggaca taagagctcc ctgtcagcgg
    32941 cagagccgta atgcagccct gccagagtgg atcaacgcgc taaagctgcg gtcagtgccg
    33001 gaaggatctt cacggcatcg ccaacaatgc cgtaatccgc gtactggaag atcggcgcat
    33061 ttttatcttt attgatggcg aaaatggttt gcgacgcgtt agcgccaacc atgtgctgga
    33121 tctgcccgga gatccccacc gccaggtaca gttcaggttt cagcatcagg ttggagatac
    33181 cgacatagcg ttcgtgctcc atccattttt cgttttctgc caccggacga gaacaggcca
    33241 actccgcacc tatcgccttg caaagctgtt ctgccagtgc aatgttctct ttgctgccaa
    33301 tgccgcgacc gacgctgacc accagacggg ctttgtcgag atcgacgctg ttgctctggc
    33361 gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc acggtgtgcg
    33421 tttcgccagt gcgtgacgcg tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca
    33481 gtaccgcata cggcgtggca atgcgttctt cgccaatcgc cagaccgccg taaaccatgt
    33541 gtttcactgt tgctttaccg tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg
    33601 ctttaaggcg ataacccagt tttgccgcca gtaatttgcc gcgacgggtg tttggcaaca
    33661 gcaccagacc gtcagcgccg tgctggcgaa tagtgtcagc catgacaccg gcgtaatctt
    33721 cgatcatccg atcgtccggt ttgccgttta atttccagac atgattagcg ccgagctgga
    33781 ttgcctgtgc gccgtcggca tcattgagga caaaggcatt gatttgatta gctaaagcct
    33841 gcgcaccgtt catcagttcc ggcagacgag agggggtatc gctgaatacc cagacttgag
    33901 aaaacgtgtt catagcatcc cctgtaatta aatgactttg cgaagatttt cagcaaatgc
    33961 ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc gctgtttcgg
    34021 cgcggcaacc tgttgttcag accaggcgtt cacggcgtta aaaccaatat ccgccgccga
    34081 ccatacctgg actggctttt tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg
    34141 ggagttgata tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta aggtttcggt
    34201 ttcatcttcc agttcgcgct caacggtgag ggtatctgcc gtcagggaga taattttgct
    34261 gacgccgtta actgccggaa tattgaggat ttcgcccacc agcagaccaa cctgctgggc
    34321 ataaaggtcg gaagaaccat cgccacagag gatcagatca aagcctgctt tctgggcggc
    34381 tgcagccagt gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt catcaatcac
    34441 cacaatcagt tcatccgggc cgcgcgatag cacatcttta cgccctttgg cgttggtcag
    34501 ggctttaccg cccacactta aggctgtcac ctgcgcctct gctgcctgtt gctttagctg
    34561 gcaagccgct tcaatagcgt tgagatcgta ttggcttatt ttggcatcgg ctttgctgaa
    34621 gtctaatgaa ccatcagcat tattgaccgc aatatcctgt tcatcaggca cgcacttata
    34681 gcaagtaata atcttcattg catctccaga aatcatgaag gaaacgtcgt tcatatcggt
    34741 agtgttaatt acagatatca cgccctttaa tttggaaata aaactttaat caccaatatt
    34801 gaaaatgtca cacgcagata aatccaactt tcaatattgt taagttcctc accaatattg
    34861 aaaacatggc gtagcaaaaa gaaattttca atattgtttt atagatcacc aatattgaaa
    34921 gtcagatgct attttttacc cacaaaagaa cataacaaac tgattaataa ccaaaattaa
    34981 cgaaaaacgt cctctattca ctcgaaacaa aaatgtgaca ccaatcacag aatacagctt
    35041 attgaatacc cattatgagt tagccattaa cgcgtccacg aggttaataa taattatatt
    35101 aaatgttaac aaaaataaaa caaacgggaa cacaaaataa atattcgttt tcacagtgga
    35161 attaactcat gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc
    35221 cattaataat cgtcggcata ctttgttggc ttacagtcag agatctggat gcggcgaatg
    35281 tcgttattaa tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt
    35341 atatggtggt gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc
    35401 gtttaggtaa cgaaccgcca gaatttagca ccgccagttg gatctttatg atgttcgcct
    35461 cctgtacgtc tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca
    35521 ccccgccgtt tggcttagaa ccgaactcga caggagcgaa agagttgggg ctggcttaca
    35581 gcttgttcca ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg
    35641 cttacttctt ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc
    35701 tggtaggtga aaaacacgcc aaagggttgt tcggtaccat cgtcgacaac ttctatctcg
    35761 tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt
    35821 gtatgcaatg gttgtttgac attccgcata ccctgcaact ggacgctatc atcattacct
    35881 gctggattat cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg
    35941 ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc atcgtcagcg
    36001 gtgccagctt catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc
    36061 cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg
    36121 tgttctactg ggcatggtgg gtgatttacg ccatccagat gagtatcttc ctcgcccgca
    36181 tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat ggtgatgggg ctgacagcat
    36241 ccacctggat cctgtggacc gtgctcggta gtaacactct gctgttgatg gataaaaaca
    36301 tcatcaacat tccaaatctg atcgaacagt acggtgtggc gcgcgccatc atcgaaacct
    36361 gggccgctct gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg
    36421 ccaccgttac gctggttaac gcctgctctt ataccctggc gatgtctact tgccgcgaag
    36481 tacgcgatgg tgaagaacca ccgctgctgg tgcgtatcgg ttggtcaatt ctggttggca
    36541 ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg
    36601 ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg
    36661 cgaaacagaa ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt
    36721 ttaatttaaa tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg
    36781 aaaactggga ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg
    36841 tcaaagcact ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc
    36901 tggacgcggg gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac
    36961 caacctatgt actgtaccag ctgccgggcg ggttcaatac cttcctgcgc gaaggcacac
    37021 aagagcagat cgacaagatt atggctttcc gcggcaccgg taagcagatg tggaactcag
    37081 cgattaccga accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta
    37141 gaaatggtaa gatttatctt aatggtagta agtgttttat taccagtagc gcctacaccc
    37201 cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat
    37261 ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa acttgagaag ctcggtctgc
    37321 gtatggatag ctgctgtgaa atcacctttg acgatgtgga actggacgag aaagacatgt
    37381 tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc
    37441 tggtagccct gaccaactac ggtacggcga tgtgcgcctt tgaagatgcg gcgcgctacg
    37501 ccaaccagcg cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat
    37561 tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga
    37621 aagcagacaa cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca
    37681 atgcggcatt tgaagtggtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg
    37741 gcaaccaccg catcagccgc ttctggcgtg acctgcgtgt agaccgcgtt tccggaggat
    37801 ctgacgaaat gcagatcctg acgctgggtc gtgcggtgct gaagcaatac cgctaagtcc
    37861 gtgcgctgcc tgatgcgacg ctgacgcgtc ttatcaggcc tacgggagca cgaatgtaag
    37921 ccggataagg cgtttacgcc gcatccggca gtcatgcgct acataaattt ttcaggagct
    37981 aataatggat catctaccca tgccgaaatt cggaccgctg gccggattgc gcgttgtctt
    38041 ctccggtatc gaaatcgccg gaccgtttgc cggacaaatg ttcgcagaat ggggcgcgga
    38101 agttatttgg atcgagaacg tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc
    38161 gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg
    38221 cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gtaaaggtcc
    38281 ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt
    38341 tatcgctcac ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc
    38401 ctataacacc atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca
    38461 gccaatgcct gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc
    38521 ggcgctggca gcactgcata aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc
    38581 catgtatgaa gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg
    38641 cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta
    38701 taaatgtgcc gacggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg
    38761 ctttaaagat attggcctcg cacatctact cggtacgccg gaaatcccgg aaggcactca
    38821 gcttatccac cgtatcgaat gcccttacgg cccactggta gaagaaaaac tcgatgcctg
    38881 gctggcggca cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg
    38941 tgccaaagta ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc
    39001 aatcactcag tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa
    39061 attcaaaaat aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc
    39121 tgccattttg aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaaagg
    39181 tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg cttgcctgac tagcgcccgc
    39241 ccaataacaa acgaacaaaa atggatagag gtgcaatgga tatcattggc ggacaacatc
    39301 tacgtcaaat gtgggacgat ctggcggacg tttacggtca taaaacggcg ctgatttgtg
    39361 aatccagcgg cggagtcgtt aaccggtata gttatcttga gttaaatcag gagattaacc
    39421 gcacggcaaa cctgttttat acgctgggga ttcgcaaagg caacaaggtt gcactacatc
    39481 tcgacaactg cccggaattt atcttttgct ggttcgggct ggcaaaaatt ggcgcgatta
    39541 tggtgccgat taacgcccgc ctgttacgcg aggaaagcga gtggatcttg caaaatagcc
    39601 aggcgtgcct gctggtgacc agtgcgcaat tttatcctat gtatcaacag attcagcagg
    39661 aagatgccac tcaattgcgg cacatttgcc tgacagatgt ggcacttccc gctgatgatg
    39721 gagtgagttc atttactcaa ctgaaaaatc aacaacctgc caccttgtgc tatgcaccgc
    39781 cgctatcgac tgacgatacg gcggaaattc tcttcacctc cggcaccacc tcccgaccga
    39841 aaggtgtggt gattacccat tacaacctgc gcttcgctgg atattactcc gcctggcagt
    39901 gtgcactgcg tgacgatgac gtctacatga cggtaatgcc tgcgtttcat atcgattgcc
    39961 agtgtactgc ggcgatggcg gcgttttctg ccggggccac ctttgtgctg gtcgagaaat
    40021 acagcgcccg cgccttctgg ggacaggcgc agaagtaccg cgccaccatt accgaatgta
    40081 ttccgatgat gatccgtacg ttgatggtgc agccgccttc agcgaacgat cggcaacacc
    40141 gcctgcggga agtgatgttt tatctcaact tgtcggagca ggaaaaagac gcattttgtg
    40201 aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac ggaaaccatt gtgggcatta
    40261 tcggcgatcg ccctagcgat aaacgacgct ggccgtcgat tggtcgggcg gggttttgct
    40321 acgaagcgga gatccgcgac gatcacaatc gcccgctccc ggcaggtgag atcggtgaaa
    40381 tctgtattaa aggcgtacca gggaaaacca tcttcaaaga gtattttctc aacccgaaag
    40441 ccactgcgaa agtgctggaa gccgacggct ggctacatac cggcgatacc ggataccgcg
    40501 acgaagaagg ctttttttat ttcgtcgatc gccgctgcaa tatgatcaaa cgcggtggcg
    40561 agaatgtctc ctgcgtggag ctggaaaata tcatcgccac ccatccgaaa attcaggaca
    40621 tcgtggttgt gggtattaaa gattcgattc gcgatgaagc catcaaagca tttgtggtgc
    40681 tgaatgaagg tgaaacattg agcgaagagg aattttttcg cttctgcgaa caaaatatgg
    40741 cgaaatttaa agtgccctct tatctggaga tcagaaaaga tctgccacgt aattgctcgg
    40801 ggaaaataat tagaaagaat ctgaaataaa tgaaacgtca gggaactacc ctgccagcta
    40861 ataatcacgc cctaaaacaa tatgcatttt ttgcagggat gttgtcgtcc ctaaaaaagc
    40921 aaaaatggag aaaaggaatg agtgaatcat tacatctgac ccgcaatgga tcaattctgg
    40981 aaattaccct tgatcgccca aaagcgaatg ctattgatgc aaaaaccagc tttgaaatgg
    41041 gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt attaccggtg
    41101 ccggagagaa attcttttcc gcgggctggg atttaaaagc ggcagcagaa ggcgaagcac
    41161 cggatgctga ctttggcccg ggtggttttg cgggattaac cgaaattttc aatctcgaca
    41221 aaccagttat cgcagctgtg aacggctacg cctttggcgg cggtttcgaa ctggcgctgg
    41281 cggcagattt tattgtttgt gccgataacg ccagcttcgc cctgccggaa gccaaactgg
    41341 gcatcgttcc tgacagcggc ggtgtgctgc gtctgccgaa gatcctgccg cctaccatcg
    41401 tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga agaggcgctg cgttggggga
    41461 tagtcaaccg cgtggttagc caggcggaac tgatggataa cgcccgcgaa ctggctcagc
    41521 agctggttaa cagcgccccg ctggcgattg cggcgctgaa agagatctac cgcaccacca
    41581 gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg aaacactatc
    41641 catcggttct gcattcggaa gatgccattg aagggccgct ggcgtttgcc gagaagcgcg
    41701 atccggtgtg gaaaggacgt taacaaccgt gagctattac gcctttgagg ggttaattcc
    41761 ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc ttgattggcg atgtgattgt
    41821 gggagccggt gtctacatcg gcccactcgc ctcactgcgt ggtgactacg ggcggttgat
    41881 cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg catggctact gcgacactga
    41941 caccatcgtt ggggaaaacg gccatatcgg gcatggagcg atcctgcatg gttgtgtgat
    42001 tggtcgcgat gcattggtcg ggatgaacag cgtaattatg gatggcgcgg tcattggcga
    42061 agagagcatt gttgccgcaa tgagctttgt caaagcaggc tttagtggcg agaaacgcca
    42121 gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt agtgacgacg agttacactg
    42181 gaaacggttg aataccaaag agtatcagga tcttgtcggg cgctgtcatg catcgttaca
    42241 tgaaacgcag ccactgaggc aaatggagga aaatcgcccc cgtttgcagg ggacgacgga
    42301 tgtgacgcct aaacggtgat cttgccggat gcgacctggt tgctggataa gatgctacaa
    42361 catcgtatct ggcaagcagg acgcccggta tcaacgagat aaaattaacg acgcatactc
    42421 tttgacaaca tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga
    42481 atacgcggtg tctttcttac gccgactaat ttttcccgac tgttattgcg caggcgcgcg
    42541 taaatttgct catcgatatc gaccacttta accagtcgct gacactggca tccccgccct
    42601 tccagcttat tagggatcat cttaacttcg cagcttattt ctgtgacttc cgacagaata
    42661 taagtcaggg tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt
    42721 gctatcaccc accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt
    42781 tcaacatatc cttcacacat acgaactcca gtttcacaaa ttaaaagtct ggctatgact
    42841 cgttttgaag caattaaaca agaccatatt aaaattgtgg atatatcgat agtatgcaac
    42901 ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca
    42961 aaagatttac ttaatgggca aaaaaaaacg gtcctcatca gagaaccgta ttagttattc
    43021 gatcaaatta gcggatgaaa aatatccgcc acgacacgct attatttgat ctgtgcgtgc
    43081 atttcctgca ccgaaattac tttttcagtc gcatcggcat tcagcgccat cgcggtagcg
    43141 aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc
    43201 acgcgggagt cttcaatcgc acgacggcct gaggtggtgt tgatgatgta ggtatattcg
    43261 ccattcttga tacggtcctg aatgtgcgga cggccttcat gcaccttgtt taccagacgc
    43321 ggattgatac ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag
    43381 ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg
    43441 gaaagcagcg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggcg
    43501 aacgcttcag cgaaggtgcg gcccacgccc atgacttccc cggtagagcg catttctggc
    43561 cctaacagcg ggtcaacgcc tgggaattta ttgaacggca gcaccacttc tttcaccgag
    43621 tagtacggcg gaataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc
    43681 acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacagta
    43741 cgcgccgcac gcgggtttac ttcaatcagg taaacttcgt tgtttttcac cgcaaactgc
    43801 acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc
    43861 acatcctgaa tttcctgact taaggtgtac gctggcagcg aacatgcgga gtcaccggag
    43921 tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg
    43981 cagatggcat ccacgtcaac ttctactgcg tcatcaagga aatgatccag caacactggc
    44041 gcatcgttag acacgctaac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag
    44101 acgatttcca tcgcccgacc gccgagaacg taagacggac gcaccaccag cgggtagcca
    44161 atctcttttg ccttctcaac tgccatttca atagcagtaa cggtggcgtt cgccggttgt
    44221 ttcagtttca gacggtcaac cgcatgctgg aagcgttcac ggtcttccgc acggtcgata
    44281 gcatccgggc tggtgccgat aaccggtacg ccagcggctt ccagtgcacg cgccagtttc
    44341 agcggggttt gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt
    44401 tccagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg
    44461 gtagagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcacgcagc
    44521 gccagcgagg cgtgtacgca gcagtagtcg aattcgatac cctgaccgat acggtttgga
    44581 ccgccgccaa gcaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct
    44641 tcataagtgg agtacatgta agcggtgtcg gtggcaaact ctgccgcaca ggtatccacg
    44701 cgcttgtaga ccgggtgcag gtcatattgg tcacgcagct tacggatttc cgcttcgcgt
    44761 acgcccgcca gttttgccaa gcgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg
    44821 aattcagcgt tcaggccagt gatgcccact tccgccactt tctcttccag acgcaccagt
    44881 tcttcaatct gtaccaggaa ccagcggtca atgttagtca ggttgaagac gccgtccaca
    44941 gacaggcccg cgcggaaagc atcggcgatg taccagatac gctctgcgcc tgcgtctttc
    45001 agttcgcgac ggattttggt taacgcttcc gggtcatcca ggctcacttt cgggtcgaag
    45061 ccagttgcgc ccacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga
    45121 ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcaccg
    45181 gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg
    45241 aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtctag ggtgtaaccc
    45301 accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc
    45361 gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc
    45421 accgcaaact ggacattgga accaccggtt tcaacgccga tttcacgcag caccgccatc
    45481 gaggcgttac gcatgatttg atattctttg tcagtcagcg tttgggctgg cgcgacagtg
    45541 atggagtcac cggtgtggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg
    45601 cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac
    45661 tcatcaatca gcaactcttt ggtcggagag agatccagac cgcgggcgca aatttcttca
    45721 aactcttcac ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga
    45781 ataatgcacg ggaagcccac gtcagcggca acagccagcg cttcttccat cgtgtgtgcg
    45841 ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg
    45901 tcttctgctt tatcaatcgc atcggcagtg gcaccaatca tggtgacacc gaactcttcc
    45961 aacacgccct gacgttccag ctccagcgcg cagttcagcg ccgtctgacc gcccatggtt
    46021 ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtaccacttc ccagtgaatc
    46081 ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag
    46141 ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag
    46201 tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt
    46261 tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt
    46321 actgctcaat taacttgata aagtggtcga acaacggcgc ggcgtcgtgt ggaccagggc
    46381 tggcttcagg gtgaccctgg aagctgaacg ccggtttatc gatacgatga atgccctgta
    46441 acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat
    46501 ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa
    46561 ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca
    46621 gcgccagcag ctgatgaccg agacagatgc cgaataccgg aatatcggtt tcgaggaact
    46681 tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga
    46741 agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg
    46801 tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat
    46861 aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca
    46921 acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat
    46981 ccatgccgtt cagacctggg aacgcgcggg ctttttctaa cgccagcgcc gcatccgggt
    47041 tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct
    47101 tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt
    47161 cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg
    47221 catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat
    47281 aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg
    47341 tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac
    47401 cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa acacctccat
    47461 agaatattca ctcactttat ttgcatatta attcacagtg atgacgtaaa tcaatgcaaa
    47521 tctgcttacc gacgaatttc tggcaaacgg cggcattctg gagatatggg cgctaaaagt
    47581 caacttaatg gcaggatttt ttatattatt ttgtggctca tgcatgtttt acaggcatta
    47641 agcgacaaaa acatcatcta acctgttctg gaaaacgctt gcgcagcaag aagattgcat
    47701 gaaataaaaa cgagcaggaa aaagtggacc aaatggtcaa aatttacatt aaaacaacaa
    47761 aaatagaaga cgaaacatgt aatttttggc agttatagaa aatttaaaag cataacacca
    47821 aaaataaaag ggctaaaaat taaagccctt tataattaat aaattaaatg tgttactttt
    47881 gcatcataat aaacattttg tggttacaaa ttattgagat caagcacatc tcgcatatca
    47941 aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcacc gttagcaaat
    48001 gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
    48061 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtaccaggc
    48121 acacgttcac cggtatggcc ttcacgactg tagaccgcgc aatctttcag atctttatca
    48181 agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
    48241 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct
    48301 ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc
    48361 gcaatatcgg cggcggcgtc acgaattgcc tgtttaccgg cttcgtcaaa ccccgtagtg
    48421 ccgatcacca tccctttgcc atgctgccga caaaaagcga gatggttcag cgtaccttcc
    48481 ggacgggtaa aatcgataaa cacatcaaaa tcatctttta tcgcatcaag actgctttgc
    48541 acggtaacgc ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa
    48601 gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag tgccgcctga
    48661 atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca
    48721 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
    48781 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
    48841 agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag gttacggcac
    48901 cagagccagc acctcagcca cccactgttg gaagcctttt acattcagat ccaatgccac
    48961 ctgtacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt
    49021 aaattcgcct tgagtttcta ccgcgacaaa acacggcttg agggtgaata agtccgggcg
    49081 caccagccag gcgatggcgc agagatcgtg cattcgcaga ccgctttgca tactgccgct
    49141 acggtagtgg ctaaacaggg cgtggagcat ttttccggtg cgattcaact ccggtaaagt
    49201 agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc acatgacaat
    49261 ttcgatgccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc
    49321 ggcatttggt gtacagttgc cgcgaccagc agaaccgccc atgatcacca gacgacgaat
    49381 atacggttta cattccgggc attgtaaaag taacagcgca atattggtta acgggccgat
    49441 ggccaccagg gtaacgggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc
    49501 cggtatcccg agtggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc
    49561 gccgtgcaca gatgctgcat cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc
    49621 gagcggaatc tccgcattcc agaaatgcag cagttgcagg gcattgcggg tagttttctc
    49681 aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gggcaaaaat
    49741 cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa
    49801 acgcatgttt tcttcataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg
    49861 ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa
    49921 acaatatttt cttcacggcc ttccagcgga atggcttcac caccacccaa ctgctgcaaa
    49981 cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg
    50041 catttaacct ctttcaccca ctcttcctgg atatctttcg catcgtcaat caaaaacgcg
    50101 tgtttgccca tacgctgggc cagctccgcc agacggttgg agttggagga gtttttcgaa
    50161 ccgaccacca acacaacttc cgcctgttct gccagggcgc gtaccgcttc ctgacggtta
    50221 gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc
    50281 agcgcgtcga tcacatcaga cgtatcatcc accgacagcg tggtttgggt cataaaggag
    50341 agcttctctt cgtttttgac cgtcagtttc cacacatcgt ctggcgattc gaccagatac
    50401 attccccctt cggggttgct gtactgcccc atcgtccctt ccacttccgg gtgcccggcg
    50461 tggccgatga gaatagattc ttcgccacgg cgactggcgc gggcaacttc catatgcact
    50521 ttggtcacca gcggacaggt ggcatcaaac accgtcaaat cgcggctttt cgcttcgtta
    50581 cgaaccgcct gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggcacttcg
    50641 ctaatctgct caataaagat agccccacgc tcgcgcaagc tatcgaccac gtagcggtta
    50701 tgcaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca
    50761 atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc
    50821 atgttacgcc tccagtgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg
    50881 cccggccagc ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac
    50941 accaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc
    51001 gcctgcatcc ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc
    51061 aaacgccgca tctggctcca gcgagaaggt ggttttgtcg cccactttca gccccagcag
    51121 gtgttgctcc agcccttcag aaagagaagc atcgcccagg cggaacagcg ccggtttacc
    51181 gttgttgcga gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag
    51241 gacggcgcta ttgctctgta cagattcaga catgaaggtt tgctcttatt tttgaggatt
    51301 tttgccggat gcggcctacg atttcgtagc aatctgtagg ccgcatccgg cagggtttat
    51361 tattgttttt tcgctttgga aggtaaaaac ccttccagca cgatcagtgc cgcaccgaca
    51421 caaatggcag tatcggcaag gttgaaggtg gcgaagtgcc agtcgccgac gtagaagtcg
    51481 atcatatcga caacgaagcc gtgccacagg cggtcgaaca ggttgcccag cgcgccgcca
    51541 ataatcagcg cgtaagcgat attgtttagc ttctgcgtgg ccttcgagcg atacatcatc
    51601 actgccagga gcacgctaat accaatcgca ataccggcaa agaaccaacg ctgccagccg
    51661 ccgctatcgg caaggaaact aaacgccgcg ccatagttac gcgcataatg cagattaagc
    51721 gacgggaaca gcgggaccgt atcccccaga gcaaagttct ggaggatcag gtatttgctg
    51781 cccagatcga taatcagcac gactaccacc agccacagcc agcgtagccc tgttgaacag
    51841 atcgattgac tcatcaggca aacttacgtt tttcaccgtc accggcgacg ttgctgacac
    51901 agcggccgca gatttctgcg tgttccgcca ccttgccgac atcctgggtg tagtgccagc
    51961 agcgtgggca cttctcacct tcggctttac tcaacgcgac tttcagccct ttgagtactt
    52021 cgctctgctg agcatcagca ggtgcgtcgt tatagtctgc aacggtagcg ccggaggtca
    52081 acaggacaaa tcgtaattca tcgcccagcg cggtcagttt cgccgccagt tccggttctg
    52141 catacaaggt tactgccgct tccagcgagc cacccacttt cttgtcggca cgcgcttgct
    52201 caatgacttt gttcacttcg ccacgcactt tcaacagctc gtcccagaac gcatcgttca
    52261 ttgcttcact gtctgccaga ccaaacaggc cttcgtacca ctcaccggtg aagacgtatt
    52321 tttcacgttc gcccggcaga tagccccaca cttcatcagc ggtgaaggag aggattggtg
    52381 ccatccagcg caccagcgct tcagcgatgt gatacagcgc agtctggcag ctacgacgcg
    52441 ccacgctatc cgctttggcg gtgtactgac ggtctttgat gatgtcgagg tagaaggaac
    52501 ccatctcaac ggagcagaag cgcatcagac gctgtaccac ttcgtggaaa tcgtatgctt
    52561 cgtacgcctt gaggatgtct tcctgtgccg ctttcgcaca acctacggcc cagcgatcca
    52621 gtaccaccat ctcttccggt ttcaccatat cttttgctgg atcaaaacca ttcaggtttg
    52681 ccagcaggaa gcgcgcggtg ttacggatac gacgatagct atcggcagca cgtttcagga
    52741 tctcgtcaga aacggccatt tcaccggtgt agtcggttga tgccacccac agacgcagaa
    52801 tatccgcgcc cagtttgttc atcacatcct gcggcgaaac ggtgttgccg atggatttag
    52861 acatcttgcg accctgacca tccacggtaa agccgtgggt cagtacctga cgatacggcg
    52921 ctttgccttt catcgcggtg gagatcatta gggaagacat gaaccaacca cggtgctggt
    52981 cagaaccttc cagatacatg tccgctgcgt gaccggcaaa ttccggacgc acgtcaacaa
    53041 cagaagagtg ggtagatccg gagtcaaacc atacgtcgag agtatccggc actttcacat
    53101 actggtcagc ttcgtcgccg aggatctctt tcgcatcgag atcccaccac gcctggatgc
    53161 cgtcaacttc aacgcgttta gccacttctt ccatcagttc gagggtacgc ggatgcagct
    53221 cttccgtgtc tttgtgcacg aacagtgaca tcggtacgcc ccaggtgcgc tgacgggaga
    53281 tacaccagtc aggacggtta gcaaccatcg actcgatacg cgcctggccc cagtccggga
    53341 tccactgcac gcctttgatc tctttcagtg actgcgcacg cagacctttc tgatccatgc
    53401 tgacgaacca ctgcggtgtc gcgcggaaga tgatcggcgt tttgtgacgc cagcagcacg
    53461 gatagctgtg ctgcattttc tcaacgtgca gcagcgcgcc tttttcctgc agcagcgcaa
    53521 cgacgatgtc gttcgctttg aagacgttca cgccatccag cgtcggataa gtgcccggca
    53581 gataagtgcc gtccgggcca accgggttag cggtttccag gccgtatttc tgaccgatca
    53641 catagtcgtc cgggccgtgg ccaggcgcgg tgtgaacggc accggtaccg gcatccaggg
    53701 taacgtgatc gccgaggatt gccggaacgt cgaagcccat aaacggatgg gtaaagcgca
    53761 gcagctcaag ctctgcacct tttaccgtgc cgagaatggt gtaatcggtc acgccgatac
    53821 gctgcattac gctttcaacc agatctttcg ccagaatcgc ggcctgaccg tcgatctgca
    53881 ccagcgcata gtcgaaatca ggtgcaatag agattgcgcg gttcgcaggc aaagtccacg
    53941 gcgtagtggt ccagatcacc agcgagattg ggccgttaac gttgcttacg ccaaatttcg
    54001 ctttcagcgc atcctgatcg accgcctgga aagcgacgtc aatggacgga gaagttttgt
    54061 cgtaatactc aacttccgct tctgccagtg cagaacggca gtctacgcac cagtgcaccg
    54121 gcttcgcgcc tttgtgcagg tgaccattgc cgatgatttt gcccagcgcg cggatgatgt
    54181 tggcttcagt tttgaagtcc atagtcagat acgggtgcga ccagtcgccc agcacgccca
    54241 gacggataaa gtctttgcgt tgaccgtcaa cctgggtcgc cgcgtattcg cggcacttgg
    54301 cgcggaactc ggcggcggtg aatttctcac ccggcttacc gtattcttgc tctactttca
    54361 gctcgatcgg cagaccgtgg cagtcccagc caggcacata cggcgagtca taaccggaaa
    54421 gccctttgga cttcacgata atgtctttca gaatcttgtt aaccgagtga ccaatatgaa
    54481 tgctgccatt cgcataagga gggccatcat gcagaatgaa ggtttttttg ccttttttag
    54541 ccgcacggat gatgccgtac agatcatcat cagtccaacg cgccagcatt cccggttcgc
    54601 gcttggcgag atcgccacgc atcgggaacc ctgtttccgg caaattcagg gttgatttat
    54661 agtcactcat cagattctcg gttccgtgtt tcggtttgat tacataacag gcttaagccg
    54721 gttttgttag cccaaaaaat tcgcgggcgg ttaattcatc acgcgcaatc tgcgctttca
    54781 gttcgtccag cgacgcaaat cgctgctcat tgcgtatttt tttacgcagc actacttgta
    54841 tatggcgacc gtaaaggtcc attgcaacat ctaacaaatg cacttccagc tgctggcgaa
    54901 taccggcaac cgttgggcgt gttccgatgt ttgccacgcc gggtaacggc ttttcaccga
    54961 ggcccagcac ttctaccgca taaacccctt tcaccgggga aacctgacgg cgtagcggta
    55021 cattcgccgt cgggaaacct atagtgcgcc ctaattcatc accgtggact acacgcccgg
    55081 agatggcaaa cgggtgcccc agtaaacttt ctgccagagc cagattgtca tcagccagcg
    55141 cctgacgcac ggcggtgctg ctgatacgca caccaccttc gcaaaaagtt tgcgtactgg
    55201 tgatatcgaa gccgtattcc atgccagctt tctgtaataa caagaaatcg ccttcacgac
    55261 cagcgccaaa gcggaaatca tcacctacgg caagaaattt tacgcgcaaa tgcttcacca
    55321 aaagatcgct gatgaaattt tgcgcggtta acgccgcaaa acgcctgtcg aaacgcacgc
    55381 acagcacgta atcaacgcca cactctgcaa ggtaacgcag tttttcccgc agccgggtca
    55441 gtcttgccgg ggctttatcg gtagcaaaca gttccagtgg ttgaggttca aaaagcatca
    55501 ccatcaccgg taagttgcgc ttgcgccctt cttcctgcaa gccctgtaac agcgcgcgat
    55561 gaccgcgatg cacgccgtcg aaattaccaa tagtcagcac acacccttct tgcggggcct
    55621 ggctgagatt atgtatgccg cgtatcagct tcatgtctgg ctcaaaacag tgaaaatcgt
    55681 ccgagtatac cttgtacagc ggtcaaggtt aaccggcgat tgagtaccga aatcagaaag
    55741 aagtggcttt tcattgccat ggcgcaaatc acgggaagaa actgaccgcc tgctgcaatt
    55801 tttatcgcgg aaaagctgta ttcacacccc gcaagctggt agaatcctgc gccatcacta
    55861 cgtaacgagt gccggcacat taacggcgct tatttgcaca aatccattga caaaagaagg
    55921 ctaaaagggc atattcctcg gcctttgaat tgtccatata gaacacattt gggagttgga
    55981 ccttggctaa tatcaaatca gctaagaagc gcgccattca gtctgaaaag gctcgtaagc
    56041 acaacgcaag ccgtcgctct atgatgcgta ctttcatcaa gaaagtatac gcagctatcg
    56101 aagctggcga caaagctgct gcacagaaag catttaacga aatgcaaccg atcgtggacc
    56161 gtcaggctgc taaaggtctg atccacaaaa acaaagctgc acgtcataag gctaacctga
    56221 ctgcacagat caacaaactg gcttaatcgc caatttgctg aagctttgtg aaaaagcccg
    56281 cgcaagcggg tttttttatg cctgctgctt ttgtgatgcg tgagagaaca cactgccggt
    56341 tgccagatag tcaaaagaga catgccgttg tactatctga actcttcgtg gcttaagaat
    56401 cgtagaacca tcggctgata cattctgaac aagaatatct actcaatctc cag
//
LOCUS       DAJTNM010000013        55387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTNM010000013 DAJTNM010000000
VERSION     DAJTNM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.013"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..354
                     /locus_tag="OO172_000885"
     CDS             <1..354
                     /locus_tag="OO172_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5511165.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO172_000886"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO172_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5511166.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO172_000887"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO172_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5511167.1"
                     /translation="MKHLHQFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="OO172_000888"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="OO172_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5511168.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO172_000889"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO172_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5511169.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO172_000890"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO172_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5511170.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="OO172_000891"
     CDS             6877..7281
                     /locus_tag="OO172_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5511171.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="OO172_000892"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="OO172_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5511172.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="OO172_000893"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="OO172_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5511173.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO172_000894"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO172_000894"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5511174.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO172_000895"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO172_000895"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5511175.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12146
                     /locus_tag="OO172_000896"
                     /pseudo
     CDS             10704..12146
                     /locus_tag="OO172_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12216..12992
                     /gene="yaaA"
                     /locus_tag="OO172_000897"
     CDS             12216..12992
                     /gene="yaaA"
                     /locus_tag="OO172_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5511176.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13145..13441)
                     /locus_tag="OO172_000898"
     CDS             complement(13145..13441)
                     /locus_tag="OO172_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5511177.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13655..14941)
                     /gene="thrC"
                     /locus_tag="OO172_000899"
     CDS             complement(13655..14941)
                     /gene="thrC"
                     /locus_tag="OO172_000899"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5511178.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14942..15874)
                     /gene="thrB"
                     /locus_tag="OO172_000900"
     CDS             complement(14942..15874)
                     /gene="thrB"
                     /locus_tag="OO172_000900"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5511179.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15876..18338)
                     /gene="thrA"
                     /locus_tag="OO172_000901"
     CDS             complement(15876..18338)
                     /gene="thrA"
                     /locus_tag="OO172_000901"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5511180.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18364..18483)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18419..18484)
                     /gene="thrL"
                     /locus_tag="OO172_000902"
     CDS             complement(18419..18484)
                     /gene="thrL"
                     /locus_tag="OO172_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5511181.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18698..19384)
                     /locus_tag="OO172_000903"
     CDS             complement(18698..19384)
                     /locus_tag="OO172_000903"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5511182.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19784..19924)
                     /locus_tag="OO172_000904"
     CDS             complement(19784..19924)
                     /locus_tag="OO172_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511183.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20020..20736
                     /gene="arcA"
                     /locus_tag="OO172_000905"
     CDS             20020..20736
                     /gene="arcA"
                     /locus_tag="OO172_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5511184.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20795..22147)
                     /gene="creD"
                     /locus_tag="OO172_000906"
     CDS             complement(20795..22147)
                     /gene="creD"
                     /locus_tag="OO172_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5511185.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22205..23629)
                     /gene="creC"
                     /locus_tag="OO172_000907"
     CDS             complement(22205..23629)
                     /gene="creC"
                     /locus_tag="OO172_000907"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5511186.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     VAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(23629..24318)
                     /gene="creB"
                     /locus_tag="OO172_000908"
     CDS             complement(23629..24318)
                     /gene="creB"
                     /locus_tag="OO172_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5511187.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSLGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24331..24804)
                     /gene="creA"
                     /locus_tag="OO172_000909"
     CDS             complement(24331..24804)
                     /gene="creA"
                     /locus_tag="OO172_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5511188.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25015..25884
                     /gene="robA"
                     /locus_tag="OO172_000910"
     CDS             25015..25884
                     /gene="robA"
                     /locus_tag="OO172_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5511189.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25881..26528)
                     /gene="gpmB"
                     /locus_tag="OO172_000911"
     CDS             complement(25881..26528)
                     /gene="gpmB"
                     /locus_tag="OO172_000911"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5511190.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26580..27101
                     /gene="yjjX"
                     /locus_tag="OO172_000912"
     CDS             26580..27101
                     /gene="yjjX"
                     /locus_tag="OO172_000912"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR5511191.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(27186..27512)
                     /gene="trpR"
                     /locus_tag="OO172_000913"
     CDS             complement(27186..27512)
                     /gene="trpR"
                     /locus_tag="OO172_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5511192.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27602..29539)
                     /gene="sltY"
                     /locus_tag="OO172_000914"
     CDS             complement(27602..29539)
                     /gene="sltY"
                     /locus_tag="OO172_000914"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5511193.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     EIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29750..31417
                     /gene="ettA"
                     /locus_tag="OO172_000915"
     CDS             29750..31417
                     /gene="ettA"
                     /locus_tag="OO172_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5511194.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31524..31691)
                     /locus_tag="OO172_000916"
     CDS             complement(31524..31691)
                     /locus_tag="OO172_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511195.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31725..32957)
                     /gene="nadR"
                     /locus_tag="OO172_000917"
     CDS             complement(31725..32957)
                     /gene="nadR"
                     /locus_tag="OO172_000917"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5511196.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32978..34360)
                     /gene="radA"
                     /locus_tag="OO172_000918"
     CDS             complement(32978..34360)
                     /gene="radA"
                     /locus_tag="OO172_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5511197.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34409..35377)
                     /gene="serB"
                     /locus_tag="OO172_000919"
     CDS             complement(34409..35377)
                     /gene="serB"
                     /locus_tag="OO172_000919"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5511198.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35483..36127
                     /locus_tag="OO172_000920"
     CDS             35483..36127
                     /locus_tag="OO172_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5511199.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36155..37171
                     /gene="lplA"
                     /locus_tag="OO172_000921"
                     /pseudo
     CDS             36155..37171
                     /gene="lplA"
                     /locus_tag="OO172_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37203..37466
                     /locus_tag="OO172_000922"
     CDS             37203..37466
                     /locus_tag="OO172_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5511200.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37627..38346)
                     /gene="deoD"
                     /locus_tag="OO172_000923"
     CDS             complement(37627..38346)
                     /gene="deoD"
                     /locus_tag="OO172_000923"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5511201.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38403..39626)
                     /gene="deoB"
                     /locus_tag="OO172_000924"
     CDS             complement(38403..39626)
                     /gene="deoB"
                     /locus_tag="OO172_000924"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5511202.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39678..41000)
                     /gene="deoA"
                     /locus_tag="OO172_000925"
     CDS             complement(39678..41000)
                     /gene="deoA"
                     /locus_tag="OO172_000925"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5511203.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLDGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41127..41906)
                     /gene="deoC"
                     /locus_tag="OO172_000926"
     CDS             complement(41127..41906)
                     /gene="deoC"
                     /locus_tag="OO172_000926"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5511204.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42164..43705
                     /locus_tag="OO172_000927"
     CDS             42164..43705
                     /locus_tag="OO172_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5511205.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDTRCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43677..44540
                     /locus_tag="OO172_000928"
     CDS             43677..44540
                     /locus_tag="OO172_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5511206.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44754..45536)
                     /locus_tag="OO172_000929"
     CDS             complement(44754..45536)
                     /locus_tag="OO172_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5511207.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45533..46606)
                     /locus_tag="OO172_000930"
     CDS             complement(45533..46606)
                     /locus_tag="OO172_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5511208.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46728..46889)
                     /locus_tag="OO172_000931"
     CDS             complement(46728..46889)
                     /locus_tag="OO172_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5511209.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(47016..47621)
                     /gene="osmY"
                     /locus_tag="OO172_000932"
     CDS             complement(47016..47621)
                     /gene="osmY"
                     /locus_tag="OO172_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5511210.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(48014..49603)
                     /gene="prfC"
                     /locus_tag="OO172_000933"
     CDS             complement(48014..49603)
                     /gene="prfC"
                     /locus_tag="OO172_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5511211.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49694..50371)
                     /gene="yjjG"
                     /locus_tag="OO172_000934"
     CDS             complement(49694..50371)
                     /gene="yjjG"
                     /locus_tag="OO172_000934"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5511212.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50386..50832)
                     /gene="rimI"
                     /locus_tag="OO172_000935"
     CDS             complement(50386..50832)
                     /gene="rimI"
                     /locus_tag="OO172_000935"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5511213.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50801..51214)
                     /gene="holD"
                     /locus_tag="OO172_000936"
     CDS             complement(50801..51214)
                     /gene="holD"
                     /locus_tag="OO172_000936"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5511214.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51317..52348
                     /gene="rsmC"
                     /locus_tag="OO172_000937"
     CDS             51317..52348
                     /gene="rsmC"
                     /locus_tag="OO172_000937"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5511215.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVITQTGRFKVYRA
                     IMTRQAKKG"
     gene            52625..52711
                     /locus_tag="OO172_000938"
     tRNA            52625..52711
                     /locus_tag="OO172_000938"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52659..52661,aa:Leu,seq:cag)
     gene            52740..52826
                     /locus_tag="OO172_000939"
     tRNA            52740..52826
                     /locus_tag="OO172_000939"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52774..52776,aa:Leu,seq:cag)
     gene            52861..52947
                     /locus_tag="OO172_000940"
     tRNA            52861..52947
                     /locus_tag="OO172_000940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52895..52897,aa:Leu,seq:cag)
     gene            complement(52986..53222)
                     /locus_tag="OO172_000941"
     CDS             complement(52986..53222)
                     /locus_tag="OO172_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5511216.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53363..54151
                     /gene="fhuF"
                     /locus_tag="OO172_000942"
     CDS             53363..54151
                     /gene="fhuF"
                     /locus_tag="OO172_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5511217.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(54189..54866)
                     /gene="bglJ"
                     /locus_tag="OO172_000943"
     CDS             complement(54189..54866)
                     /gene="bglJ"
                     /locus_tag="OO172_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5511218.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQEFFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(54824..>55387)
                     /locus_tag="OO172_000944"
     CDS             complement(54824..>55387)
                     /locus_tag="OO172_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5511219.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        13258 a        14946 c        13826 g        13357 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaaatc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagaaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagagaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cgctaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aaggcagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcgt catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaattgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcta ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggtg
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcacc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ttctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataagg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggagctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcattcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagcggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagcg tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggagcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcgccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc acgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacagt ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccgccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgcgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tattgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 cgggaatggg atcgacgcca aacgcggcag cggcagcggc agcggcagcg gcatcctggc
    11581 ctccgcatcc ggcagcacaa gggattgtcc agatgattgg catttttatc gacaccctgg
    11641 tcatctgtac ggcaagcgcc atgctgatat tactggcggg taacggcaca acctacatgc
    11701 cgctggaagg tattcagctt atccagaagg cgatgcgggt gctaatgggt tcctagggtg
    11761 ctgaatttgt taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca
    11821 tttatgccga aaacaatctc ttctttttac gcctgaacaa ccctaaagcg atctggtgtt
    11881 tgcggatctg caccttcgca acggtcatcg gcggcacctt gctaagcctt ccgctgatgt
    11941 ggcaactggc agatatcata atggcctgca tggctattac caatttgacc gccattttac
    12001 tgctctcgcc tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg
    12061 tgcgcccggt gtttgatccg ttgcgttatc cggatatcgg tcgccagctt tctccggacg
    12121 cgtgggatga tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg
    12181 ctaaagtcgg cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga
    12241 aaacgcttga ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg gagctgttag
    12301 acaattccca gcagttgatc catgaagcgc ggaaactgac gcctccgcag attagcacgc
    12361 tgatgcgcat cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc
    12421 cagatttcac gccggcgaat gcccgccagg cgattctggc gtttaaaggt gatgtctaca
    12481 ccggcttgca ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc
    12541 gaatgctttc cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc
    12601 tggaaatggg gatccgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag
    12661 atatcatcac caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtggtgatta
    12721 atctggcgtc agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta
    12781 tcaagccggt gttcctcgat gagaagaacg gcaaatttaa gatcatcagc ttctacgcta
    12841 agaaagcacg cggtctgatg agtcgtttca ttattgaaaa tcggctgacc aaaccagagc
    12901 aactgactgg ttttaatagc gaaggttact tctttgatga agattcctcc agcaatggcg
    12961 aactggtgtt taaacgctac gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg
    13021 aacgccttat ccggcctaca aaagcatgca gattcaatat attgcagaat taacgtaggc
    13081 ctgataagac gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac ccggcatttg
    13141 tcatttagcg gtgatgtttg cctggaccat gaccgccgtg atgatcatga ggagctttct
    13201 tatggtggcg cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct cgccattcat
    13261 aatgttgttt ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa tagccacgat
    13321 tatcacgatc gcctatctgt aattttactg acgggactaa cgtaatttcc gcagcctgtg
    13381 ctgccatggg agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca
    13441 caggttactc cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga
    13501 acgacggttg ttattctccg caatcctaat ctctaagtta ccgcatttat tgataatttc
    13561 tcctttttcc ctgtcgtttt tctccataat ttcttcataa aaaagccggg ctgcataaaa
    13621 gcaaacccgg cctgattgag ataatgaata gattttactg atgattcatc atcaatttac
    13681 gcaacgcaga aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca gcacgttctg
    13741 ccagctcttt tggcagatcc aacgtttcac cgagaatcgc ttccacgctc tctttaaatt
    13801 tcgccggatg cgcggtgccg aggaacaagc catattcgcc tggattcaac tggtcacgca
    13861 gcgcacgata cgctacggca gcgtgcggct ccgaggtgta gcccagttct tttaactcac
    13921 gcattgtctg ttgcgtggtt tcatcatcca cggctgcata acccagctct ttcagttgcc
    13981 agattttgcg gcggaacaac tcttccacac gcggccagtt gttcggctga ctcacgtcca
    14041 tcgcgttgga taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac
    14101 gtggcacggt atcgttcacg ttggtcgcag caataaaacg tttcaccggc agaccgaatg
    14161 acttcgccag cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca
    14221 gctggttgcg cgcctcctgc ggcagctgcg caacagcttc aaagtagtag caaatctgcg
    14281 ccagcaaacg gctgatgtta atcgagttag ccgagtttaa ccccagcgcc actttcagtt
    14341 cttcatcatc aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg
    14401 caaccgtttc gatattgccg cccaacgtac agaacagttt ttcttgcagt ggactgattt
    14461 tgcctcgtgg atagaggata accactttca cattcggcaa accgtagaaa gcatgagcca
    14521 ctgcagctcc ggtatcaccg gaggtcgcgg tcagaatggt cactggctta tcatccgcaa
    14581 tatgggtcag catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc
    14641 cgtggaacaa ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg
    14701 caaacgccgc gcgcacgcgc tcttccagga tttcctgtgg gatttcatca ccaataaacg
    14761 ccgagaggat cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag
    14821 tcaggctgaa ttccggcagg tcgtgcggaa aaaacagccc ctgatttttg cccaacccct
    14881 gggttacggc ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg tagagtttca
    14941 tttagttttc cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt
    15001 cctgattttg caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct
    15061 tgtcacatag agcgaacaag gtcgggccgg agccggagat accgctcgct accgcgccga
    15121 tttccgcgac cgcctgccgc gcctgccgga agccaggcag taaccgttca cggtagggtt
    15181 cagcgataac atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt
    15241 gaatgaagcc agccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg
    15301 gtaaaatagc ccgggcttct gccgtcgaga ctttaattcc cggatacgcc agcacccaca
    15361 gccactcatc aaaccctggc acttgctggc tgatgatgtc gttttcttcg atcatcaact
    15421 gcataccacc aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc
    15481 cttccagctc gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg ccgcagtgtt
    15541 cattcatcgc catcagcgcc gcgaccaccg aacaggcgct ggagcctaag cccgaaccga
    15601 tcggcatatt cttttccaga gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa
    15661 aacgctccca gcactgataa acgatatttt cccgtggttc tgacggcagc ttatcggcaa
    15721 agcgtccgag gttgttgaga ctgaatgtct ctgccgcctc aaccgtgact acatctccga
    15781 gcaatgcacc atcaacgggt gccaccgccg ccccgagcac atcaaacccg acgctcatat
    15841 tggcactgga agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg
    15901 gtacgtagca gatcggcaaa gacaccggca gcggtaacgt cattgcccgc accatatccg
    15961 cgcagcacca acggcagcgg ctgataatag tggctataaa aggccagggc gttttcgcca
    16021 tttttcactt tgaacagcgg atcattccca tccacttcgg caatcttcac gcggcagaca
    16081 ccatcttcat caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttcgcc
    16141 acgcgcgcgg caaagagatc gtcgagctgt gacagattcg ccataaaagc ggcaacatca
    16201 ccctcggcgt taaactctgc gggcagcaca ggttcaattt caatatccgc cagctccagt
    16261 tcacgtcccg tttcacgagc gagaatcaat agtttacgcg ccacatccat accagaaaga
    16321 tcatctcgcg ggtccggttc ggtataaccc atttcccgcg ccagcgtggt cgcctcggag
    16381 aaactcatgc cttcgtctaa cttgccgaag atataagaaa gcgaacctga aagaatgccg
    16441 gagaacttca ccaattcatc acctgcattg agcagatttt gcaggttctc aataaccggt
    16501 aatccagccc caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcataa
    16561 cgcaactgat gatagtaatc catcgacgag gtgttggcct ttttgttcgg cgtgacaacg
    16621 tggaaacctt cgcgcaggaa gtcggcatat tgatccgcca ctgcctggct ggaggtgcag
    16681 tcaacaatga ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga
    16741 ttaaacggct ctttggcttg tgcaagttct tcctgccagt tttccagatt aaggccatgc
    16801 acactagtga gcagagcctt cgagttggca acgccgcaga cacgtaagtc gatatgttta
    16861 ttcttcagcc agctttgctg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg
    16921 acgccaatca caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg
    16981 cgcacgccag tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat
    17041 ccctgagcaa tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc
    17101 gagatcccac gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc
    17161 gtcaccgcca gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt
    17221 gcccgttcag ctcgcacaca gtcgctttgt ggaacgcaga aactgatact gtattcggaa
    17281 gatgattgcg taatcagcac cacggaaata cgggcgcgtg acattgcagc aaagacgcgc
    17341 gccgccatgc caaccatccc tttcatcccc gggccggaaa cgctgaacat tgccatgtta
    17401 ttcagattgg aaatgccctt gaccggtaat tcgtcttcat cacggctggc accaatgagc
    17461 gtacctggtg cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg
    17521 atgggggtaa tggtgcgggg gtgaagaact ttagcgccga agtaggaaag ctccatcgct
    17581 tcctggtagg acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta
    17641 tagaccccgt caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc
    17701 accgccgcgg agtagtcgga accgttacgt ccaagcacca ccagttcgcc tttttcatta
    17761 ccggcggtga aacctgccat caacaccatg tgatcagccg gaatgcggct tgccgcaata
    17821 cggcgggtgg actcagcaat atcgacggta gattcgaggt aatgccccac tgccagcagt
    17881 ttttcgaccg gatcgataac ggtaacgttg tgaccacgcg cttctaacac gccggccata
    17941 atggcgatcg acattttctc gccacggcaa atcagcgcag cgttgatgct atccgggcac
    18001 tgccccaaca aactaatgcc atgcaggaca tgttttattt gggcaaattc ctgatcgacg
    18061 aaagttttca attgcgccag cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt
    18121 tcggcaaaaa tacgttcggc atcgctgata ttgggtaaag catcctggcc gctaatggtt
    18181 ttttcaatca tcgccaccag gtggttggtg attttggcgg gggcagagag gacggtggcc
    18241 acctgcccct gcctggcatt gctttccaga atatcggcaa cacgcagaaa acgttctgca
    18301 tttgccactg atgtaccgcc gaacttcaac actcgcatgg ttgttacctc gttacctttg
    18361 gtcgaaaaaa aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc
    18421 agcccgcacc gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt
    18481 tcatggatgt tgtgtactct gtaattttta tctgtctgtg cgctatgcct atattggtta
    18541 aagtatttag tgacctaagt caataaaatt ttaatttact cacggcaggt aaccagttca
    18601 gaagctgcta tcagacactc tttttttaat ccacacagag acatattgcc cgttgcagtc
    18661 agaatgaaaa gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa
    18721 tatcatgcag caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt
    18781 gctgtaacca gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc agcaatgcca
    18841 tgactcgttc gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca cttttcgccg
    18901 gttgttgtat taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga
    18961 gcgaaggata atccgccacc atcggcacac cagtaagcac gtcagccaac gctaactctt
    19021 cgttagtcag cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag
    19081 atttttcctc taacagcggc accagttcta ctggcgtggc gtagtaatga tatttcgccc
    19141 gactacgcgc agtggtggcg acagtgaaat cgacatcgtg taacgattca gccagtgtcg
    19201 ggaaaacttt aatattatca ataatatcac tagatccatg tgcgacccag cgggtggctg
    19261 gctccaggtg tgcctgacta tcgacaatcc gcagttcgct aaaacccatc gttttcattg
    19321 cccgcgccgc tgccccaata ttttctgctc tggcgggtgc gaccaggata atcgttatac
    19381 gcatattgcc actcttcttg accaaataac cgcgaacagg gtgatcactg tcaacttatt
    19441 acgcggtgcg aatttacaaa ttcttaacgt aagccgcaga aaaagccctt tacttagctt
    19501 aaaaaaggct aaactatttt ctgactgcac taacggttta attgttaaaa aatgctacat
    19561 atccttctgt ttacttagga taattttata aaaaataaat ctcgacaatt ggattcacca
    19621 cgtttattag ttgtatgatg caactagttg gattattaaa ataatgtgac gaaagctagc
    19681 atttagatac gatgatttca tcaaactgtt aacgtgctac aattgaactt gatatatgtc
    19741 aacgaagcgt agttttattg ggtgtccggc ccctcttagc ctgttatgtt gctgttaaaa
    19801 tggttaggat gacagccgtt tttgacactg tcgggtcctg agggaaagta cccacgacca
    19861 agctaatgat gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta cgtactttag
    19921 tcatgttacg ccgatcatgt taatttgcag catgcatcag gcaggtcagg gacttttgta
    19981 cttcctgttt cgatttagtt ggcaatttag gtagcaaaca tgcagacccc gcacattctt
    20041 atcgttgaag acgagttggt aacacgcaac acgttgaaaa gtattttcga agcggaaggc
    20101 tatgatgttt tcgaagcgac agatggcgcg gaaatgcatc agatcctctc tgaatatgac
    20161 atcaacctgg tgatcatgga tatcaatctg ccgggtaaga acggtcttct gttagcgcgt
    20221 gaactgcgcg agcaggcgaa tgttgcgttg atgttcctga ctggccgtga caacgaagtc
    20281 gataaaattc tcggcctcga aatcggtgca gatgactaca tcaccaaacc gttcaacccg
    20341 cgtgaactga cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct gggtactgtc
    20401 agcgaagaac gtcgtagcgt tgaaagctac aagttcaatg gttgggaact ggacatcaac
    20461 agccgttcgt tgatcggccc tgatggcgag cagtacaagc tgccgcgcag cgagttccgc
    20521 gccatgcttc acttctgtga aaacccaggc aaaattcagt ctcgtgctga actgctgaag
    20581 aaaatgaccg gccgtgagct gaaaccgcac gaccgtactg tagacgtgac gatccgccgt
    20641 attcgtaaac atttcgaatc tacgccggat acgccggaaa tcatcgccac cattcacggt
    20701 gaaggttatc gcttctgcgg tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa
    20761 cggcgctttt tagcgccgtt tttatttttc aaccttattt ccagatacgt aattcatcgt
    20821 ccgttgtaac ttctttactg gctttcattt tcggcagtga aaacgcatac cagtcgatat
    20881 tacgagtcac aaacatcatg ccggccagcg ccaccaccag cacactggtt cccaacaaca
    20941 gcgcgctatc ggcagagttg agcagtcccc acatcactcc atccagcaac aacagcgcga
    21001 gggtaaacaa catgctattg cgccagcctt tcaataccgc ctgcaaataa ataccgttca
    21061 ttaacgcccc aatcagactg gcgattatcc atgccacggt aaaaccgata tgttcagaaa
    21121 gcgccagcaa gagcaaataa aacatcacca atgaaagccc caccagcaaa tattgcattg
    21181 ggtgtaaacg ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa
    21241 tcagcagaat ggcgtactta gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg
    21301 tcactgcgac gctaaacgcg gggaagtttt cccagccggt atcattgcct gaagcaaaac
    21361 gctcaccgag attattagca aaccagctgc tttgccattg cgcctgaaaa cctgactcgc
    21421 tagcttcccg tttggctggt agaaaatcac ctaaaaaact gggatgcggc cagttgctgg
    21481 ttaaggtcat ttcgctatta cgcccggcag gcaccacaga aagatcgccg gtaccgctta
    21541 aattcagtgc catattcagc tgcaggttct gcttccgcca gtccccttcg ggtaaaggga
    21601 tatgcacgcc ctgcccgcct tgctctaacc cggtaccggg ttcaatggtc agtgccgttc
    21661 cgttaacttc aggcgctttc accacaccaa taccacgcgc atccccgacg ctaatcacaa
    21721 taaagggctt gcctaagatg atatttggcg cgttgagctc gctaagacgc gaaacatcga
    21781 aatcggcttt tagcgttaaa tcactgtgcc agacctgacc ggtataaatc cctatcttgc
    21841 gttcttccac gttctgattg ccatcaacca tcaatgactc aggtaaccaa aaatggataa
    21901 aacttcgttt ccgctccacg gttttatcct cttcctgcac cgtataaagc tcggtcacag
    21961 gaatagcgat aagcggccca acgagttttt gcggcccgct ggtactttgg cgaatcgcat
    22021 cttccacatc gctacggtaa tcggcacgtt cgacaatcac ttgccgaatc agcattatcg
    22081 gaatcaacaa cagcaatact gcaccaaaca aggtggtcat tttccagaac aggggggatt
    22141 tcaacatagt cttctccttt gcaatggcag caggatacga aggctatgtg ggaagaattt
    22201 gaagctatgt gaagttacgg tgaagtcgta gcgaggccag cacgccacct tcctgcacgt
    22261 tgcgcagcgt gacttcgccg ttaaacaaac gggcgacctc actgacgaac gccaacccca
    22321 gaccgctgct tttttgccca tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg
    22381 acagcgcgta gtcaggaata ccactaccgg tatccagcac cttaagcgtg acgtgttcct
    22441 gatccacttc ggcgcttagc gttatgcaac cgctctcggg agtaaaatcg atggtgttat
    22501 ccagtaaatt ccccagcgcc tgatccagta acgccggttc agcagcaaca ttaacctcgg
    22561 tgggcataac atgcaaagtg atgttttttt ctgccaactg cacggtgcgc gcttcgctga
    22621 cgcggcgaaa taatgccgcc acatcaacag cagtcagaac gacttcctga cgattctcca
    22681 gtcttgcctg gcgtagtaac gtttccacca gcgcctgcat tcgcgcattt tgtgtcagaa
    22741 tgttgtcagt aaaacgagcc actacttcag gcggcggacc ttcgcgtaaa atttccgcca
    22801 cgccacgaat cgccgccagt gggcttttta gctcatgggt taacgcataa acatactgct
    22861 caatatagtt tttcccttcc agcttcacgc gcatactttc cagcgcctgc gcgagtttac
    22921 gcagctcgct actaccgaga tcgggaagag gaacgggctt attgtcagtg acggaatcag
    22981 catagcgagt gagccgggca atggagcggt tgatccacca aaccatgcct gcgccaatca
    23041 ccagtgcaat ccccaacaaa atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa
    23101 tgaccggagc catcgccgcg ttcggtttgc ctacgctcaa aacgccaata agccgcgagc
    23161 cgcccataat cggcgcggca acatacatca ccgaactttc gggatcggca ggattttgca
    23221 acgtgctgcg cgcaccatac tgaccacgca acgttagcca gacgtcattc cagcgcgaat
    23281 aatcctgtcc aacggcttta tttgccgaat cgaacaatac tttgccatgc gcatcggtca
    23341 tatagacatg atattcattg cgcactttgt taatgccacc aatattggcg cgaaacgggc
    23401 gatgttgtag ctgattaaac gcctgcgcca gttgcccatg cgttgggtcc ccagagagca
    23461 aatcgggacg cgccagctcc gcaagtaacg ttgccgtgtc gatcaacgtc ccctccgttg
    23521 ctcttcgcac gcccggttta acttctttga caaaaatagc cagtacgaac caggccgcca
    23581 ccgccaccag taaaaaatag cccagcagca accgcatgcc gatacgcatt acaagcccct
    23641 caggctatat cccatgccgc gatgagtatt aatcggtgaa agatcggggt tgatggcgcg
    23701 cagcttggca cgcagcgttt taatgtggat atcgacggtg cgatcgtagg tgtcctgcgc
    23761 atcttcccac acgctatcca ttagttgctg gcgggaccag acgcggccta gcgacttgag
    23821 taacgtcttc agcaataaaa actcataccg cgtcagcgtt aatggcgtgt caaaccagct
    23881 gatctgcgcc gcgggttcat tcaattcaaa atgaccaata cggatgacgg gagacggcgt
    23941 cgagaacttc ttcacccgac gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga
    24001 aaacggttta gccacatagt cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc
    24061 ttcacttcgg gccgtcagga acagtacagg taacgccgga tggagcgcca gtaactggcg
    24121 gcacaattca aagccactga tatcaggcaa accaacgtcg agaatcatga cgtcgggaac
    24181 ctgctggcga gctttatcca gcaccggcaa acctcgctca aagacctcga cggcaaaacc
    24241 ttcctgctgc aacatgtaga ccagcgtgtc ggctatccct tgctcatctt ccactaacca
    24301 gaccgtttcc cgttgcataa atcgcctctg ttattgccgc cacggcatga caggaaccgc
    24361 gctaatcgcg tttttcggcg aaccttctac aactttgtcg gagtaagcca gataagcgag
    24421 cgcgttgcgt ttggcgtcat aaaagcgcac gacctgtaac gacttaaaga ccagggacgt
    24481 gcgttttttg aatactacct cgccctgagc tttgccgttt ttaatacgat ccgacagttc
    24541 aatcggcccg acttgctgac aagaaatggc cgcatcggag gtatcttctg ccaggcccaa
    24601 tcccccttta ataccaccgg ttttcgcccg gctcacataa caggtgacat ttttcacatc
    24661 gggatcgtca aaggcttcca caacaatttt gtgatccggg ccgatcattt taaatacggt
    24721 gtcgaccgaa ccaatctctt ctgcatgagc caatggcccc agcattatca ggcttaaaga
    24781 caggatcaaa tgcttgtatt tcatattgtt accattttta tctattacac gagcaattag
    24841 ttcgtcacgg taaataacat attgattaag aacaaaatct caattctttt atttccgtca
    24901 ggccctaaaa catactctac taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct
    24961 attatccaat tacctgatgt caggtgctcg ttgttgaaag gatgaggata ttttatggat
    25021 caggccggca ttattcgcga ccttttaatc tggctggaag gtcatctgga tcagcccctg
    25081 tcgctcgaca atgtagcggc gaaagcaggt tattccaagt ggcacttaca gagaatgttt
    25141 aaagatgtca ctggccatgc tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg
    25201 gcggtcgcac tacgcctgac tgcgcgtccg attctggaca tcgcgctgca ataccgcttc
    25261 gactctcaac agacatttac ccgcgcattc aagaagcagt ttgcccagac tcctgcactt
    25321 taccgccgtt ctcctgaatg gagcgccttt ggtattcgcc cgccgctacg tctgggtaaa
    25381 ttcacaatgc cagagcacaa atttgtcacc ctggaagata cgccgctgat tggtgttacc
    25441 cagagctact cctgttcgct ggagcaaatc tctgatttcc gccatgaaat gcgttatcag
    25501 ttctggcacg attttctcgg caacgcgccg accattccgc cggtgctcta cggcctgaat
    25561 gaaacgcgtc cgagtcagga taaagacgac gaacaagagg tattctatac caccgcgtta
    25621 gcccaggatc aggcagatgg ctatgtactg acggggcatc cggtgatgct gcagggcggc
    25681 gaatatgtga tgtttaccta tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg
    25741 gtatacggaa cgtgcatgcc aatgctcaat ctgacgcgcc gtaaaggtca ggatattgag
    25801 cgatactacc cggcagaaga cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa
    25861 ctgctgattc ctatccgccg ttaacgctgc agctcatcta atgcaggggc gtccagatgc
    25921 gagatgtccc ctgcggtttc aacgacccag ccagacgcca gccacaggct ttcctgataa
    25981 tccacgcgcg aaatagaaca gttacgcaga cgtaagcggc gctctgccca tgctggtaat
    26041 ccgagaatcg tactcaccag acatcccagt gcaataccat ggctcaccag cagcggtcgg
    26101 cttccctgcg gtaagtcccg gcaagattcc agtgccgcat tgacgcgatc gctgagctcc
    26161 tgcattgact cgccttcagg aatacgcccg tcaacggtgc cattgaccag ctgccgacgc
    26221 cagttctctt cttcttcggt cagagaatcg atatgtcttt tttccagcac acccatgttt
    26281 aattcacgca ggcgagaatc aaagatgatg tcacagccgc aggcctgggc gatgatttcc
    26341 gccgtacgcc gggtgcgtcc taaatcgcta ctgataatat gcgtaatgcc aagctctttg
    26401 gcacgggttg ccacctgcat cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac
    26461 tggccctgaa tacgtcgctc ggcgttccac tgcgtttcac cgtggcggac taggtatacc
    26521 tgtaacatgc tttttttccg tatactgctg taacgttaat tttctgagta atgctgatta
    26581 tgcaccaagt tgtctgtgcg accaccaatc ccgctaaaat tcaggccatt ctgcaggcat
    26641 ttcacgagat cttcggcgaa ggatcctgcc atattgcatc cgttgccgtc gagagcggtg
    26701 taccggaaca accctttggc agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa
    26761 atgcccgccg tttacttcca gaggctgatt tttgggtggc gattgaagct ggcattgatg
    26821 gcgacagcac tttcagttgg gtggtgattg aaaacgccag ccagcgcggc gaggcgcgtt
    26881 cggcaacttt gccgttacct gcggtgattc tggaaaaagt tcgtgaaggc gaggcgcttg
    26941 gtcccgtgat gtcgcgttat accggtattg atgagattgg ccgtaaggaa ggggcgatcg
    27001 gcgtgtttac cgccgggaaa ctcactcgcg ccagcgtcta tcaccaggcg gtgatcctgg
    27061 cgctgagtcc gtttcacaat gccgtgtatc aggcattgta ggacggataa ggcgttcacg
    27121 ccgcatccga cattcggtgc acgatgcctg atgcgacgct gccgcgtctt atcaggccta
    27181 caaaatcaat cgcttttcag caacacctct tccagccact gacgcagctc cacgggcgcg
    27241 gctttcaggc tgttagatcc acgcgtaatc gtcgcgatgc ccgcgccgag ttcatttttt
    27301 aactcacgct gactcatttc gccacgcaac agctcttcga caatacgcac gcgagtcccc
    27361 aacgcttcgc gctcatctgg cgtcagcatc aggtttaaca acggtaaatg gagatcgttt
    27421 tggtaggcat tcttaagcag gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc
    27481 atcgctgctg aatagggtga ttgttgggcc ataatatgtc gccattgtta gcgggggaag
    27541 caaaatgact cccccggttg tactcgctaa agagtacgat agcatatcat aaacgtgcgg
    27601 atcagtaacg acgtccccat tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat
    27661 agcggtagta agcatcataa gccagcacgt tcttcacata accgcgcgtc tcggagaacg
    27721 gaatactctc gacaaatgcc acagcgtcga tacgcccggc gctgttgcca agccaggttc
    27781 gcacccgtcc aggtccggcg ttataagctg ctgaggagaa aatacggtta ttgccaaact
    27841 gctgataaac atattgcagg taactggtgc caatgttgat gttcgtttcc ggatccagca
    27901 attgcccagg gctgctataa ccaggaatag agaacatctt caccgtatgg gtcgctgtac
    27961 caggcataat ctgcatcagg ccgctggccc ctaccggtga tttcactttc ggattccagg
    28021 cgctctcctg acgggcaatc gccatcgcat agctttgcgg gatctctttc ccactggtgt
    28081 aacgtttgaa aagatcgttg taagccagcg ggaatcgctc ttccagatga tcccaaagct
    28141 tcccggcgat cgttgcctga acgctaagat cccaccattg gttattgaaa gcataccgag
    28201 ccagttgagc ctgctctgtt tttgacttgc tcttcaccag attggcccac tcgctacgcg
    28261 cggtattatc gagattccag tacatcaact cgcgcacgcg cgccatctcc ggcccctgag
    28321 tcagggtgct gtcaacattc tgcggcgctt tatcaatctt cagctcatac tcttcaccga
    28381 tgcgttgtgc tgcaaccatc gggtagaaac cacgctgttg catgagttga tgcaaaatct
    28441 ctttcgcttc agcttcacgt ccgcgttcca gcaataaatc cgcctgccag taacgccatt
    28501 catctttctc ttttgcttcc atcggcagac gcgccagcca ggtattcagg ccgcgacgat
    28561 cgccggtgcc aagcgccatt cgcacacggc gttcaatcaa cgacgtggat tgcgagcgca
    28621 taatggcgtc atcgcgccat ttcgcctgct catcggtgac atcattgccc atcaaacgcc
    28681 aggcgacgat atcgcgcagc tcctgaatct catcttcatt aagctgctgc gcctgggcaa
    28741 gcgacgggat catcagccgt gcattttcgg catcctgccg cgccacactg gcaaacgcca
    28801 ccgccgccat ttgacgggta aaatcggtcg cgccggttgt acgcgcgaag gtcagtaccg
    28861 tattagggtt gttcgccagt gaaatgattg ccgaggcgat agtctggtaa tcggcaggca
    28921 tctgccctgc cagcactgtt accaggcctg tgttacccgc tttcatcgcc agacggatac
    28981 gctctaaata cgccagcgga tcttgtttac ctgacgcacg ccagacgcta aataacttgt
    29041 cacaggcgtt aggctggctc ttgccggtta gccacagctc tttcgcccct tgccaggctt
    29101 cttcactctg cccggtgttc catttcgcat agtagtaatt acattgcgct tcggtagttc
    29161 cgggcttttc cgggctaaag gctaacaagc cacgccagtc ttcacgccgc gccagttcat
    29221 tgacgaaacg agattgcagc gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt
    29281 tagtgaccgt caccgccggt tgattcatca ggtcatcggt gatctggcgg tattccaggt
    29341 agggataaag cggataatcc ttcagtccag gcatcatttg ttcgaccaca tccatctgtc
    29401 gattatccca ggcctgcttg atttgcgcgt aacggctacg ctgctcatcc agtgagtcgg
    29461 ctcgcgccac gctgctgacc gtcagcagac agacaccggc agccaacagc cgccaggtaa
    29521 cttgtttggc tttttccaca agcgcatcct ctaagtgtaa atacatcaat gcagcgtcat
    29581 accgcgtaat agatttatgc taaccagtca ttgccgttta cgccacgtta cggacacttt
    29641 tttactttta ctgcgaaggt gatcgaccaa ctgggattag gcggggaaac aggctacact
    29701 tcgcctttga aaactcatca tcacgataaa catagaggcg aagtccaacg tggctcaatt
    29761 cgtttatacc atgcatcgtg tcggcaaagt tgttccgccg aaacgtcata ttttgaaaaa
    29821 catctctctg agtttcttcc ctggggcaaa aattggtgtc ctgggtctga acggcgcggg
    29881 taagtccacc ctgctgcgca ttatggcggg cattgataaa gacatcgaag gtgaagcgcg
    29941 tccgcagcca gacatcaaga ttggttatct gccgcaggaa ccgcagctga acccggaaca
    30001 caccgtgcgt gagtccattg aagaagcggt ttcagaagtg gttaacgccc tgaaacgcct
    30061 ggatgaagtg tatgcgctgt acgccgatcc ggatgccgat tttgacaagc tggccgctga
    30121 acaaggccgt ctggaagaga tcattcaggc tcacgacggt cataacctga acgtacagct
    30181 ggagcgtgcg gcggatgcgc tgcgtctgcc agattgggac gcgaaaatcg ctaacctctc
    30241 cggtggtgag cgtcgtcgcg tggcgttgtg ccgcctgctg ctggaaaaac cagacatgct
    30301 gctgctcgac gaaccgacca accacctgga tgccgaatcc gtggcgtggc tggaacgctt
    30361 cctgcacgac ttcgaaggca ccgtggtggc gattactcac gaccgttact tcctcgataa
    30421 cgttgcaggc tggatcctcg aactggaccg cggtgaaggt attccgtggg aaggtaacta
    30481 ctcctcctgg ctggagcaga aagatcagcg tctggcgcag gaagcttcac aagaagcggc
    30541 gcgtcgtaag tcgatcgaga aagagctgga gtgggtacgt cagggaacta aaggccgcca
    30601 gtcgaaaggt aaagcacgtc tggcacgctt tgaagagctg aacagcaccg aataccagaa
    30661 acgtaacgaa accaacgaac tgtttattcc acctggaccg cgtctgggcg ataaagtgct
    30721 ggaagtcagc aacctgcgta aatcctacgg cgatcgcctg ctgattgatg acctgagctt
    30781 ctcgatcccg aaaggggcga tcgtcgggat catcggtccg aacggcgcgg gtaaatcgac
    30841 cctgttccgt atgatctccg gtcaggaaca gccggacagc ggcaccatca ctttgggtga
    30901 aacggtgaaa ctggcatcgg ttgatcagtt ccgtgactca atggataaca gcaaaaccgt
    30961 ttgggaagaa gtttccggcg ggctggatat tatgaagatc ggcaacaccg agatgccaag
    31021 ccgcgcctac gttggccgct ttaactttaa aggggttgat cagggtaaac gtgttggcga
    31081 actttccggt ggtgagcgcg gtcgtctgca tctggcgaag ctgctgcagg ttggcggcaa
    31141 catgctgctg ctcgacgaac caaccaacga cctggatatc gaaaccctgc gcgcgctgga
    31201 aaacgccctg ctggagttcc cgggctgtgc gatggttatc tcgcacgacc gttggttcct
    31261 cgaccgtatc gccacgcaca tcctggacta ccaggatgaa ggtaaagttg agttcttcga
    31321 aggtaacttt actgagtacg aagagtacaa aaaacgcacg ctgggcgcag acgcgctgga
    31381 gccgaagcgt atcaagtaca agcgtattgc gaagtaatgc gtaaaatgcc ggatgcggcg
    31441 cgaacgcctt atccggccta caaaaccagc aatttcaata cgttgtaaaa ctgtaggcct
    31501 gataagacgc ggtaagcgtc gcatcaggca ttgagcaccg aatgccggat gcggcgtgaa
    31561 caccttttcc ggtctacaac agccttagca tcctcaattt cgtggcgggt cacccgtgca
    31621 ggttttttca aaccttgttt aatctcgaca gcctcttcca gagaggtttt caactcatca
    31681 aaaaaactca ttttatacct cgcctttgag cagtttcgtc acggttatcc ttgctccccc
    31741 atcatctccc gcaccagctc cacgcagcgc aggaaacggc tgtcgtaatc gtcctcttca
    31801 acccgcacga attcgatatt gttctcttcc agcatctcca ccagcaagtt ctggaactct
    31861 ttgcgatcca ccgaactgcc gaggctgcgt aaaccatccg ccacccacgg cgtgttgttc
    31921 tccagcagga tcaccagatc gaaacggtat tcatcaatca gcgcctgcac gaacggatgc
    31981 tcacgccctt cgtacttttt gcagaacgcc tgggtagtga caaaatcggt gtcaataaac
    32041 gccactttat tggcgtattt cactgcaaaa tcaatgtatt gtgcgtggcc caacgcgatt
    32101 ttatcgtagt cggaatactg caatgcgatc tcatcaccgc cgaggtgtga aaagacataa
    32161 tcgcggccat attcccacgc actggtggtg ttgaagatat tggcaagttt gtttaccagg
    32221 gtggatttac cgctcgactc gccgccaagg atcgccacgg tacgcacaaa aaacggcttc
    32281 acttcggtag gaatatattc ccagtagcgg aacgggtttt cgcggatctg cgcaccgctg
    32341 atactcataa aggtacgttt cggatcgacc agcaccgtct cgatccccag atgttccata
    32401 tactgtggcg catcggcttc ttccgaggtg tagatcaggt ccggctggat ccccttttca
    32461 gccataaact ttttgatgcc gttgctccac acatcccagc catgcggata cggctccatg
    32521 ccctcttcgt tgaaagcatg aatacggata tttttctgat atttaaaggt ttgcaatagc
    32581 cagcgcagac gatccggcac cgtgggctgc tgcgacatgg cgctgtcttc aaacaacgcg
    32641 cggtcgcggg tatcgtcaaa acccataatg atatgcagct cgtcaacctg gctacaggcg
    32701 cgctggataa ggtagatatg tccggtatgc agcgggtaga acttaccgaa tacgacacca
    32761 atggttttct tctgccgggg aaattcaagc cccaaaaaac ggtgcaatgc ctccagcttt
    32821 tgcgcgctgg ggcttttaat tttggcattc agtaactggc ttaaataccc tttggtcata
    32881 ccgctggcat cagctacctg ctgtagcgtg cagccctgtt gcttgatggc agttttcagg
    32941 taatcaaatg acgacatatc tccctccgta tctctcatta taagtcgtcg aacacgctaa
    33001 gcgcgtcgga gagtttttta acgccaaaaa tctgcatccc ttccggcgct tttttcggta
    33061 cgttagccgc cggaacaatc gcccggcgaa aaccgtgttt cgccgcttca gaaatccgct
    33121 cctggccact gggcaccggg cggatctccc ctgccagccc gacttcacca aacaccacca
    33181 gatcctgtgg cagcggtctg tcacgcaggc tggaaaccat cgccagcagt aacgctaagt
    33241 cggcgctggt ttcggttacc ttcacgccgc cgaccacgtt cacaaacaca tcctgatcgg
    33301 ccatttgcag accaccgtga cggtgcaaga cagccagcag gattgccaga cggttttgtt
    33361 ccagccccac tgccacgcgg cgcggattcg ccattatcga gtgatcgacc agcgcctgaa
    33421 tctccaccag caacggacgc gttccttccc acaccaccat cacggagctg ccggaggtca
    33481 cttcatctcc gcgacttaag aaaattgccg aagggttgct gacttcacgc agcccctgtt
    33541 cggtcatcgc gaagacgccc agctcattca ccgcgccgaa gcggttttta tggctgcgta
    33601 aggtgcgaaa acgagagtcg gcatcgccat ccagaagcac cgaacagtcg atacagtgtt
    33661 ccagcacttt cgggccagcc agcgaaccat ctttggttac gtgtccaacc atgacaatcg
    33721 ccacaccgcg cgttttggcg aaacgcgtca gataagccgc cgtttcacgc acctgcgcca
    33781 cgctaccagg cgatgattgt acatccgcca tatgcatcac ctggatcgag tcaattacca
    33841 tcagcttcgg ttgctcttct tcggcaatca ggcagatctg ctcgatgctg gtttccgaca
    33901 acatattgag attgtcagtc ggcaggccaa ggcgatgagc gcgcattgcc acctgttgca
    33961 gcgactcttc gccggtgaca tacagcgttt tcatctgctg ggccagtttg cacagcgttt
    34021 gcagcaacag cgtggatttc cccgcaccag ggttaccgcc aatcagaatg gcacttcctg
    34081 gcaccacgcc gccgcctagt acgcggtcga actctttaaa tccggtggaa aaacgcggca
    34141 gctcttcaag gctgatatcg gagagtttct ggacttttgc caccccggcg ctaccggcat
    34201 agccgctgag acgctcgtta cgcgccaccg ttggcgacgc agcaagacgc acctcggtga
    34261 tggtgttcca ggcatgacag gcactgcact gcccctgcca gcgcggataa tcggccccgc
    34321 attcattaca aacaaaggcg cgttttggag cttttgccac ggtgtacctc gttaatgctg
    34381 tgccgcccgc aggatgacgg gcaggcaatt acttctgatt caggctgcca gagaggatgc
    34441 agaatacccc catcaggtca gcgtgacgga tggtgacttc cgccttttca ttcacttttg
    34501 gcttggcatg gtaggcaatc cccagccctg ctgctttgat catcggcagg tcattagctc
    34561 catcgccaat cgccacggtc tgcgccagcg ggatttcata ctcctgcgcg aggcgagtca
    34621 gagttttcgc tttgtactgt gcgtctacga tgtcgccgat cacattgccg gtaaatttac
    34681 cgtccatgat ctccagttca ttggctacca cggctgtcag gcgcagcttg tcgcgcaggt
    34741 attcagcaaa gaaagtaaag ccgccggagg caatcgccac tttccagccc agcgtttcca
    34801 gcttgagcac cagctgcgtt aagcctggca tcagcggcag attttcacgc acctgttgca
    34861 gaatattggc gtcagcgcct ttcagcgtcg ccacgcggct gcgcaggctg gcggtaaaat
    34921 cgagttcgcc gcgcatcgcc cgttcggtta cctccgctac catctcgccc gttccggcca
    34981 gcttggcaat ttcatcaata cattcaatct ggatggcggt ggaatccatg tccatcacca
    35041 gcaaacccgg cgtgcgcagg tgcgggattt tccccagcgg ggcgacatcc agctgcgctt
    35101 cgtgggccag gcgtgtagcc cgtgcggtga gtgaacctgc cagacgaatc acctgataat
    35161 cttccacgca ccaggcggca acaatcacca tcgccgcacc cagtttgctc tggtattggg
    35221 tcagacgctg tttatccagc ccacgaccat acagcagcca gccgctacga gctgcgtggt
    35281 aatccagtgg catcacttca tcaccactta atgaaagagg cagacccggc cataaagaga
    35341 catcttcagg caggtcgcac caggtaatgt taggcattaa ggctcctgta aaatcgttcg
    35401 aagcagggaa aataacgcat gaggctacct tgtatccatt gcttctggca acattaagtc
    35461 tcaaattttc aaagggtgga agatggctcg cacaaaactg aaattccggc tacatcgggc
    35521 agtgattgtc ctgttctgtc ttgccttgtt agtggcactg atgcagggag cgtcatggtt
    35581 tagtcaaaac caccagcgac agcgtaatcc acagctggaa gaactggccc gcaccctggc
    35641 gcgtcaggtg acgctgaacg ttgcaccgct gatgcgtacc gactcaccgg atgaaaaacg
    35701 cattcaggcg atcctcgatc agttaacgga tgaaagccgt atcctcgacg cgggtgtgta
    35761 tgacgaacaa ggcgatctta tcgcacgttc tggcgaaagc gtcgaagtgc gcgatcggct
    35821 ggcgctcgac ggtaaaaaag caggcggcta ttttaaccag cagattgtcg agccaattgc
    35881 gggtaaaaac ggaccgctcg gctatctgcg cctgacactc gacacccata cgctcgccac
    35941 cgaagcccaa caggtggata acaccactaa cattttacgc ctgatgttgc tgctctcact
    36001 ggcaatcggc gtagtgctga cccgcacgct gctacagggt aaacgcaccc gctggcagca
    36061 atcgcccttc ctgttaaccg ccagcaaacc ggtgccggaa gaggaagaaa gcgagaaaaa
    36121 agagtgaccc attactacaa gaaaggaaat cgttatgtcc acattacgcc tgctcatctc
    36181 tgactcttac gacccgtggt ttaacctggc ggtggaagag tgtatttttc gccaaatgcc
    36241 cgccacgcag cgcgttctgt ttctctggcg caatgccgac acggtagtaa ttggtcgcgc
    36301 gcagaacccg tggaaagagt gtaatacccg gcggatggaa gaagataacg tccgcctggc
    36361 gcggcgcagt agcggtggcg gcgcggtgtt ccacgatctc ggcaatacct gctttacctt
    36421 tatggctggc aagccggagt acgataaaac tatctccacg tcgattgtgc tcaatgcgct
    36481 gaacgcgctc ggcgtcagcg ccgaagcgtc cggacgtaac gatctggtgg tgaaaaccgt
    36541 cgaaggcgac cgcaaagtct caggctcggc ctatcgcgaa accaaagatc gcggcttcca
    36601 ccacggcacc ttgctactca atgccgacct cagccgcctg gcaaactatc tcaatccgga
    36661 taaaaagaaa ctggcggcga aaggcattac ttcagtgcgt tcccgcgtga ccaacctcac
    36721 cgagctgctg ccggggctca cccatgagca ggtttgcgag gccataaccg aggccttttt
    36781 cgcccattat ggcgagcacg tggaagcgga aatcatctcc ccggacaaaa cgccagactt
    36841 gccaaacttc gccgaaacct ttgcccgtca gagtagctga gagtggaact tcggtcaggc
    36901 tccggcattc tcgcatctgc tggatgaacg ctttagctgg ggcggcgtgg aactgcattt
    36961 cgacgttgaa aaaggccata tcacccgcgc ccaggtattt accgacagcc tcaaccccgc
    37021 gccgctggaa gccctcgccg ggcgactgca aggctgcctg taccgcgcgg atatgctgca
    37081 acaagagtgc gaagcgctgt tggttgactt cccggaccag gaaaaagagc tacgggagtt
    37141 gtcgacgtgg atagcggggg cggtaaggta actcgctgat taaaatatcc aaggaaggaa
    37201 atatgatcga tctggaaaac caggaacggg agattatcaa tttaatgttc agccagcgta
    37261 tttcctggct agccgccgtg cgtatcaggc ataaattatc gcttgctgaa gtaagtaaaa
    37321 tgttaggtat ctcgattaat tctctaaaac agatcgaaaa gacggaacgg cttagctcaa
    37381 acatcaaaag caaaatggca gaaatttatg gttgtccacc agagttgctg atatgccctt
    37441 cctggatgac ggctgaacat aaatgaatag attcgtgaat agatacttga atgtatttta
    37501 ttttctatcc atatgaaaaa taatagtaaa tctataaatc agttgctatt gatgaataga
    37561 tactaaaaag ccggagcagt cccccactcc ggcttaacac gacaatcgcc tttcagcgaa
    37621 acacaattac tctttatcgc ccagcagaac ggattccagt gcgattttga tcatgtcgtt
    37681 gaaggtggtc tgacgctcag cggcagtggt ctgctcgtga gtgcggatgt ggtcagacac
    37741 ggtgcagatg gtcagggctt tcgcgccaaa ctctgcagcc acgccgtaga taccagccgc
    37801 ttccatttcc acgccgagga tgccgtattt ttccatcacg tcgaacattt cgccgtccgg
    37861 agagtagaac aggtcagcgg agaacaggtt acccacgcga gcatcaacgc ccagtgcttt
    37921 agctgcatct actgcgttac gcaccatgtc aaagtcagcg atagcggcaa agtcatggtc
    37981 tttaaaacgg atgcggttaa ctttggaatc ggtgcaggcc cccataccga taacgacgtc
    38041 gcgcagtttt acgtgcggca gaactgcgcc acaggaacca acgcggataa ttttcttcac
    38101 gccgaaatcg gtgatcagtt ctttggtgta gatggagcag gacgggatac ccataccgtg
    38161 acccattacg gaaattttgc ggcctttgta agtaccagtg aagcccagca taccgcgaac
    38221 gttgttcact tcacgggcat cttcaaggaa agtttcagca atatacttcg cacgcagcgg
    38281 gtcgcctggc atcaaaacta cgtcagcgaa atcgcccatt tctgcattaa tgtgtggggt
    38341 agccattgtt ttatcctttt tgtgacataa caaaggcgga gtcaacgctc cgcccaaatc
    38401 catcagaaca tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc
    38461 tggccgatat ccgcgaaggt ttcacggtga cccagtgagc ccggttttac tttcgggcca
    38521 tataccagta ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcgcaa
    38581 ccgtggtcag cggtgaggat caggatgtcg tcatcgcgca gcagagacat cagctccggc
    38641 agacggcggt cgaacagttc cagacccgca gcataaccgg cgacgtcgcg acggtggccc
    38701 caggaagagt cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc
    38761 atctctttga tggtggcgtc aaacagcgcg tccaagccag tcgctttcac ttttttggtg
    38821 ataccgcagt tggcgtagat gtctgcaatt ttaccgacag aaaccacctg gccgtgtttt
    38881 tcatcaacca gtttctgcag cacggtcggt gctggcggct caacagccag gtcgtgacgg
    38941 ttaccggtac gctggaagtt accagctttg tcaccgataa acggacgagc gataacacga
    39001 ccgatattgt agccgccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt
    39061 ttatccagac cgaaagtttc ttcatggcag gcaatctgga acacggagtc agcggaggta
    39121 tagaaaatcg gcttgccggt tttcatgtgc tcttcgccca gttgatccag aatgaccgta
    39181 ccggaagagt ggcagttacc gaggtaaccc ggcagattag cgcgttcgac cagtttatcc
    39241 agcagctctt gcgggaagct gttttcgtga tcggagaaat atccccactc aaacagaacc
    39301 gggacaccgg cgatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc
    39361 gcccatgcgt acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg aatgaaaccg
    39421 gtagaacctt cgtgtgcttt cgccagcccc agacggatca gatttggcag attgagcggc
    39481 cctttacgac cattatcagc ttcgcctttg gcacaagctt ctgcgatatg acccagggtg
    39541 tcagccccga cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc
    39601 agcaccataa taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg
    39661 atcagatcag tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt
    39721 gcgttatcgg caagtttaat tgccgctttc accgctttcg ccgcttcctg ccagctgttt
    39781 tcgtctttcg cgaggataac cgccagcgga cgctgaccgt ctacctggtc gcccagacgc
    39841 gccatatcag taaagccgac gctgtaatcg atggtgtcag atgcctgacg acgaccgccg
    39901 cccattgcaa ccactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg
    39961 gtatcagcat agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca
    40021 acgaagtcgg tcgggccttt ttgtgccgct accatacgac caaagacttc tgccgcttta
    40081 ccgttgtcca gcaccgcctg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg
    40141 ccggagatca gcatctccac gcacagcacc atcgtgacat caaacagacg cgggttacga
    40201 tactcacccg tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag
    40261 gccagtacct gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc
    40321 acgccaacaa tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg
    40381 ctacccactt tcacgtccat caccagcgcg tccagacctt ccgccagttt cttcgccagg
    40441 atcgaggcag tgatcagcgg gatggagtcc acggttgcgg taatatcacg ggtcgcgtag
    40501 aaacgtttat cagccggagc cagtgaactg gtctgaccga taatcgccac gccgacgtct
    40561 ttaataattt cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc
    40621 agtttgtcga gcgtaccgcc agtatgaccg aggccgcgac cggagatcat cggaatatag
    40681 ccgccgcagg ctgcgaccat cggccccaac atcagcgaag tgacatcgcc gacgccgccg
    40741 gtcgagtgtt tatcaacaat cgggccatcc agatgcaggc ttttccagtc gagaacggtt
    40801 cctgaatctc gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag
    40861 aaaatggtca tcgcgagggc ggcaatctgc ccttcggaaa tagtgttgtc gcgaataccg
    40921 ttgataaaga aacgaatttc ttcatcgctc agcgcatgac catcacgttt tttacgaata
    40981 atttcttgtg cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc
    41041 ggatgcgtct acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc
    41101 agcgcatcag gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcgccgtgg
    41161 cccagcgctt tcagcaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca
    41221 tcagcccagt cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc
    41281 gcagtacgca cgccgcccgc cggtttgaaa ccaacggttt tttctacgcc catatcacgg
    41341 atcacttcca tcatgatgcg agcgctttcc ggcgtcgcgt tcacagccac tttaccggta
    41401 gaggttttga tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc
    41461 gcttcgtctt tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg
    41521 caagcctctt tacaggcttt caccaggtca aaaccaacct gctcgtcacc cgccatcagc
    41581 gcgcggtacg ggaataccac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt
    41641 tctgccagcg cgatttcgat gtcgtcgtta ccgtgtggga agttggttac cgtagcgata
    41701 cggatttccg gggtgccctg ctctttcaga gttttgcgag caatcgggat aaaacgagga
    41761 tagatacaga tagcggcggt attgccgacc ggagttttgg cctgatgaca cagagcaatt
    41821 actttctcgt cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt cagtgcacgc
    41881 aggctgcttg ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac
    41941 cttgcgagtt tgttagtatt ctaacatcta ctccgcaaca cacttcgata cacatcacaa
    42001 ttaaggaaat ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac
    42061 tttcaaatga atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac
    42121 ttcttacaat ggcgcatcac cttcggcata aaaggaaaag aagatgccca cttctcatga
    42181 aaatgcactg caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca
    42241 gaagcgccat tttctggcac tggaagcaga aaacaatctg ccttacccac agcttcctgc
    42301 cgaagcccgc cgcgcgctgg atgaaggtgt aatctgcgat atgtttgaag gtcatgcgcc
    42361 gtacaaaccg cgctatgtct tacccgatta cgctcgtttt ctggcgaacg gttccgaatg
    42421 gctggagctg gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta
    42481 ccaccacgta ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt
    42541 gcaaccgtat gttagaattc taacacaaaa cgagatcgat gttcgaataa aacgtttctg
    42601 gcgttacctc gacagaaccc tgccagatgc ctttatgcac gccaatatcg gcccgtctga
    42661 ttcgcccatt acccgcgcga tcttacgtgc agacgcgcag gtttcaccga acctgacctt
    42721 tatctacgat cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg
    42781 tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg
    42841 gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct
    42901 ggtacgcctt aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac
    42961 gcgcactcta ccgcactact gccagcagca gatcgccatc atcgatacgc ggtgtgaatt
    43021 cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa
    43081 ccctgaacgt tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct
    43141 gtgtgagaaa gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtggg
    43201 ttatcgcatc agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggctg
    43261 gcaaaaacgc gccatgttac acgcacagtc ggggatcagt tccgatatcg gcaccacgcc
    43321 gggcgcgcgt ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc
    43381 cccgcatcat gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat
    43441 caaacgtaac ccgcaggcac tggtacagct ttgcctcggt gcctttaaag ccggaatgcg
    43501 tgaatttacc gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtgcg
    43561 tttgtcggat ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg
    43621 cgaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga
    43681 acagcagatg cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca
    43741 gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt
    43801 ggacgatggg acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg catcaggcgt
    43861 tgcagattgt tgacggcaaa gtggtgtgga acgctgtggt ctgcgagcaa tgtgatacct
    43921 gcctgaagat gtgtccgcaa catgccacac caatggcgca atccatgagc gtggacgaag
    43981 tgcttagcca tgtgcgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg
    44041 aagtcacgac ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc
    44101 aactgcgcca tctcacctgt ctggtggaca gtaacggtat gttgagcgaa accggctggg
    44161 aaaaattact cccggtgtgt gacggcgcaa tgctcgatct caaagcatgg ggtagcgaat
    44221 gtcatcaaca attaaccgga cgcgataatc aacagattaa gcgcagcatc tatttgctgg
    44281 cagagcgcgg caagctggcg gaactgcgtt tgctgatgat cccaggccag gtggattatt
    44341 tgcaacacat cgaagaactg gcggcgttta tcaagggact tggagatgtt ccggttcgcc
    44401 tgaacgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc
    44461 cggaagacgt tgagccgttg gctgatgcat taaaggtgcg cggggtgagc cggttgatat
    44521 ttccggcgct ctatttgtga catgccggat acggcctacg gttcggcaca gacttgtagg
    44581 cctgataaga cgcgtcaagc gtcgcatcag gcattgtgcg ccaactgccg gatgcggcgt
    44641 gaacgcctta tccggcctac ggttcggcac aaacctgtag gcctgataag acgcgttaag
    44701 cgtcgcatca ggcattatgc gccgactgcc ggatgctgcc tacgaagggc aaactaccca
    44761 cgaacgttaa acaccgcata cgtgttatta agcaacactt cggcaatctc atccgcgggt
    44821 tccgggcgta actcgcaaag cacggcaaac acacgggcag cctgttccgg gcggttaggc
    44881 tgcccctgaa aaccgttgag cggcatatcc ggcgcatcgg tttccagcaa caacgatgcc
    44941 agcggtaatt ttgcgatgac atcgcgggtt ttactggcgc gtggataggt gatagtaccg
    45001 cctacgccga ttttgtagcc cagctgtaca aaccgttcag cctgttgctg gctgccagaa
    45061 aatccgtgga caacaccagc acgcggcaaa tcgtggcgtt taagatgcat cgccagtttg
    45121 tcgtgcgtgc gccgtgaatg caggatcacc ggcagatcgt agcgtttcgc cagtttcagt
    45181 tgttcgtcga gtaaccactg ctgcctctca aattgcggat cgtcgccgaa gagatccaga
    45241 ccgatctccc ccaccgccac caccttcgcc ggacgccttt ccagcgcctg ctgtagctgc
    45301 tcaagagaca catcgctatg tttttccaac ataccaggat gcaagcccaa tgcggcatac
    45361 agcggttgat aattttccgc taacgcctgc acgcgggcaa aattttccgc ctctgtcgcc
    45421 ggaacaatga tcttgcctac gcccgcttgt gccgcgcgtt gcaggctggc ctcttcatcg
    45481 ccaataaaag gcgggaaatc aaaatggcag tgagtgtcga taaaacggca aatcaagcca
    45541 gatcctcatt gttaaatgtc gggtcgttcg cctgcggagc gtcgctcact tcggcaacca
    45601 gcgtatcgtt ggcgactggc gcaggcggaa tgacaatcga ttccggcgtc accactggca
    45661 ccagatggcg ggtaagcggc gctttttcag ttaatagctt gccaaccgtg gcgaggaaat
    45721 aacgaccgca taaacgccca agtttatagt cttcacgcag cgccggaatc cgactgccaa
    45781 gcgcgatact atgtaatggc ttcggcggat aaatttcgga tatccgcagc ttgcccggtg
    45841 gtttctcaat aaattgctga atgtcacgat agctggtttc atgatgctgc accagattga
    45901 ccagcggctg caggctactg tcacccagcc agcgttccat gcgtttgaac cactgcggcg
    45961 tgtagtacat ttgtgacggc acagtgcgga tgacgaccaa cgttttagcg ccctgccttg
    46021 ccgcctcttt aaccggaatc gcatcactga tcccgccatc caggtagtta atgccttcca
    46081 gcgacactcc gctacgataa aagccaggta tcgcactgga ggcgcgaatc acatccagcc
    46141 agttttgttt ggttggtaaa aagtaattcg gcgcgtagtc atcctgacga caggcgcaca
    46201 tataaaacga tttgccgctg tcaaacaacc gcgcggcggt atccatttgc aacggcatct
    46261 ggcttgcggt ggcctccacc agccagtcga gatcgataag atttcctcca cggacaaagc
    46321 gcaatggatc aaaaaattcg cgttttgtgg tatagcgcat gatgactttg cgcgcgtaac
    46381 cgggctgatt gcagataaac gccgagaggt tctgcgcccc ggcagatgtg ccgagataaa
    46441 gatcgaaagg attaaactgc gcgcgcataa actcatccag cacgccagcc gtgaaaattc
    46501 cacgctgtcc gccgccttcg cacaccagag ctattcgtcc aggctggaac ggccttagcg
    46561 acaacggcgc aatattacca agcgttacag gtattctctg ccccacttct gccttcctgt
    46621 ttttgttgtg aaagaaagac aaagtaacgc aatttattat tcgctaaaac cgcaaaagcc
    46681 agtccgctgg actggctttg gataaaattt tcttaatggt ctgaaatcta gggtcgtttt
    46741 cggcccatga acaaactcac taggaacaga ataatcccga cgacaaagac aattttagct
    46801 gcgcctgcag cggtaccggc cagaccacca aacccaagtg cggcggcgat taacgcgata
    46861 accagaaata tgatgcccca acgaaacata agcgtctcct ttaccatagt taatgtcacc
    46921 gcttgatatg agcgtgaaaa tcactcaatg cgatattttt agtgtggtgc acatcgcgcc
    46981 tcccgacaaa gttcgggagg acgaattacg acgaattact tagttttcag atcattttta
    47041 acgcttttca caccatctac cgctttggcg atactttcag cacggtcact ttgtgcctga
    47101 gaatctacgg taccggagag ctgaaccacg ccatcggtgg tttcaacttt cactttacgt
    47161 gaagggacga tatcgtccgc cagcagtttg gctttgattt cactggtggt ggcggtgtca
    47221 cccgcgtagt ccttcaccga gccttcttta gcgtcgcgaa cgtgcagttt gtcgctgaca
    47281 gaggtcacgc cttcaacgcc tttcgccact ttcactgcct cttcggcctg ggcctgactt
    47341 tcaacgaaac cgctcagggt cacgactttt tgatcggttt tgacagagat atcggtgctc
    47401 ttgatgttgt catgatccac cagcgccgcc ttcactttcg cggtgatggc gctgtcatcc
    47461 atgaaattac cgactttatt catagagcta tcgacttttt gccctgcgct ttcattggta
    47521 gtctgcgcgt tgttttccgc gtaggcagag ccggtcgcga cggcagaggt caacattaca
    47581 gccagcagag ttttcgaaat cttcagtctt gtcatagtca tcgatttatt cctgtatgtt
    47641 tgctcgtaat ttgagcctag caacacgagg ttgcattgct gaatagggag agacttcacc
    47701 ctctacagaa gtcaatggtc gccatcacaa aagcgatggg tgatgaataa cgaccattac
    47761 agtctctgaa tcagttatta atatcggtag aatgacaatc gacggcttta gatactgata
    47821 tctacgcatt gaacggtatt taacgccatc agaaatgtca tcactttgtt aaatatagat
    47881 cacaattttg aaaccgctcg ggatatcagc gaaaacataa gcaaaagtga atgttttaag
    47941 aacattccgt aagcggctaa taaggaaggg aaattgacag ggcgcagcgg ctaccgcgcc
    48001 ctggaggcaa gaattaatgc tcgcgggtct ggtggaactg aacgtccgga taacgttcct
    48061 gtgccaggcg caggttgacc atgctggtag cgatgtaagc gaggttatcg ccgccatcaa
    48121 gcgccagttg gctttcgttc ttacgcttga actcttcgaa tttcttcgcg tctgcacatt
    48181 ctacccagcg ggcagtggca acgttgactg actcatacac tgcttcaacg ttgtattcgc
    48241 tcttcaggcg cgctaccacc acatcaaact gcagcacacc aaccgcacca acgatcagat
    48301 cgttgttgga gattggacgg aacacctgca ccgcgccctc ttcggaaagc tgtaccagcc
    48361 ctttgagcag ctgtttttgc ttcagcggat ctttcaggcg gatacgacgg aacagttctg
    48421 gtgcgaagtt cggaataccg gtgaacttca tcatctcacc ctgggtaaag gtgtcgccga
    48481 tctgaatggt gccgtggttg tgcaggccga ggatatcgcc cggatacgct tcttcaacgt
    48541 gcgaacggtc acccgccata aaggtcagcg cgtcggagat caccacatct ttcgcagtgc
    48601 gcacctggcg cagtttcatg cctttttcat atttaccgga caccacacgc ataaacgcca
    48661 cgcggtcgcg gtgtttcggg tccatgttgg cctgaatttt aaatacgaag ccggtaaatt
    48721 tatcttcgct cgcttctacg gtacgggtat cggtctgacg cggcatcggc gcaggcgccc
    48781 actccaccag gccattcagc atatgatcaa cgccgaagtt acccagcgca gtaccgaaga
    48841 ataccggagt gatttcgccc gcaaggaaca gctctttgtc gaactcgtta gacgcgcctt
    48901 taaccagttc cagttcgtca cgcagctgct gtgccagatc ttcaccaacc gcagcatcga
    48961 gatccgggtt attcagccct ttaacaatgc ggacttcctg aatggtgtgc cctttaccgc
    49021 tctgatagag ataggtttca tctttataaa ggtggtaaac gcctttaaac agcttgccgc
    49081 agccaattgg ccaggtgatc ggtgcgcagc caattttcag ctcgttctca acttcatcga
    49141 gcagttccat cgggtcgcgg atatcacggt caagtttgtt cataaaggtg aggatcggcg
    49201 tgtcgcgcag acgggtaact tccatcagct tacgggtacg atcttcaaca ccttttgcgg
    49261 cgtcgataac catcaggcag cagtccaccg ccgtcagggt acgataggta tcttccgaga
    49321 agtcttcgtg ccccggggtg tcgagcaggt taaccaggca atcgtgatac ggaaactgca
    49381 tcacagacgt agtaatggag atcccacgct gcttttccat ctccatccag tccgacttag
    49441 cgtgctggtt ggaaccacgg ccttttactg taccggcggt ctgaatggcc tgtccgaaca
    49501 gcagcacctt ctcggtgatg gtagttttac cggcgtccgg gtgagaaata atggcaaaag
    49561 tgcggcgctt cgccacctct tgcaaataag gagacaacgt cataattctt tcttcttgag
    49621 taagcggcat cgcgccgcgc ttgttgaaaa cgaaaaattg cggctatttt acccatcaac
    49681 gggggggagg caatcagtgt ttacacagga gctgctccag ttcgtgcaac gaagaaacgg
    49741 tccaggtggg cgcgatgcct tctggttgct cgcgattgtg cgcattcagc cagcaggtcg
    49801 caagcccggc gttgatgcca ccgagaatat cggactcggc agtgtcgcca accatcagca
    49861 cgcgtgaacg gtcaggattg cccgcctgtt ccagcgcata atcgaaaatt ttcttattcg
    49921 gtttggcaac gccaacttct tcggaaatca ccagcaaatc gaagtaatca cgcaggcccg
    49981 tccgttccag acgcacttgc tgcaaggcgc taaaaccgtt ggtgatgatg ccgattttag
    50041 cgttgccacg aatggcgtta agcagagaaa ccgcgcccgg caacggcgta cagatttccg
    50101 ccatcgcatt aataaaggca tcgttaagca agcctgcttc aactttcagc cgttcggccc
    50161 agctctcgaa acgcccgtgc tgaagctgta atgaagtgat cgcgccgttt tgataatcca
    50221 cccacagtgg cttgttaacg gcctgatagt cctgaaaatc ttcagcggta aaggtgacgc
    50281 tgtaatcaag aaacatccgc tgcaggccgg tgaatgagtc aaaggtaaac agcgtttcat
    50341 cggcatcaaa gaaaatccag tcccacttca ttattccacc ttgtcttaca tactgattgg
    50401 caacgccatg atgatggcgt cttcgcgacc gtccgtggtg gggtagtaat tgcggcgaat
    50461 cgtcgcctcg ttaaagccta aactttcgta cagggcaatg gcggcagcat ttgaggcacg
    50521 gacttccagc catagtgtcg ccacgccgcg tttttccagt tcgtcgatca gatgttccag
    50581 cagcgcccta cccagcccct gacgctgata gtcgggatcg accgcaatat tgaacaatgt
    50641 agcttcatcc agcaccactt gcgtaatcgc aaacgccgcc attttgccgt tttgcgttaa
    50701 ctgaaagttg agataacgct cgccctggtt gctggcaaag gttttttcac tccacggaaa
    50761 ggcgtgggcg cgttgttcaa tgtggtaagc cgctggtaaa tcagtcgttt cgagggaaga
    50821 aatcgtgttc atatgtgcaa atttgttgcc ataacgcggc gcgtgccgtt gggtttgccc
    50881 gtaattcggt gagcgccggt gatgccacct gagcgccttc caatgatagc ggttcgtcag
    50941 tacccaaccg ccagctgttg cagcgactgc cttgcggcag catcgcgatt ttttctggcg
    51001 tcagttgcag cacttgatcg gggctgacgg ttaatgcgcg cagaacatcg ctcactaaag
    51061 gatcagtcag ggcgggaaga tcgtttgcca ccatcaccag acggacgtgt gccgggctcg
    51121 caatggcaat ctccccctgc aacgcgccag ggcgacgcag cgaccactgg gtaatgccca
    51181 gttgctgtaa ctgccagtct cgtcgggatg tcatagcgaa tcgctcctgt tgtcaggggc
    51241 gcaaatatag caaattcgtc gataccgcgc caacatatgg ctataatcgc cgccagtatc
    51301 aattgaggag cattccatgt ctgcatttac cccggcaagt gaagtcttgc tgcgtcacag
    51361 tgatgatttc gaacaaagcc gtattctgtt tgccggagac ttacaggatg acctgcccgc
    51421 gcgtttagat accgcggcca gccgtgctca tacccagcaa ttccaccatt ggcaggtgtt
    51481 aagctgccag atgggggata acgcccgttt tagtctggtc gccacggcga atgatgtcgc
    51541 agattgcgat acgctgattt actactggcc gaagaacaaa ccggaagccc agttccagtt
    51601 gatgaattta ctttctctgc tgccagtggg gacagatatt tttgtcgttg gcgagaaccg
    51661 cagcggcgtg cgcagcgctg agcagatgct ggcagattat gcgccgttga ataaagtcga
    51721 cagcgctcgt cgctgtggcc tctattttgg tcgtctggaa aaacagccag tatttgatgc
    51781 ggataaattc tggggcgaat acagcgtcga tggcctgacg gtcaaaacgc tgcctggcgt
    51841 gtttagccgc gacggtctgg atgtcggtag ccagttgctg ctctcgacgt taactccgca
    51901 cacgaaaggt aaagtgctgg atgtcggctg tggcgcgggt gtactttcag ttgcctttgc
    51961 gcgccattcg ccgaaaattc gtctcacctt gtgcgatgtc tctgccccgg cggtagaagc
    52021 cagccgcgca acacttgcag ccaacggtgt tgaaggtgaa gtctttgcca gcaacgtctt
    52081 ttccgaggtg aaaggttgtt ttgatatgat catctccaac ccgccgttcc acgatgggat
    52141 gcaaaccagc ctggatgcgg cgcaaacgct gattcgtggc gcggtgcgtc atcttaatag
    52201 cggcggcgag ctgcgaattg tagcgaacgc cttcctgcct tacccggacg tgctggatga
    52261 gacatttggc ttccatgaag ttatcacgca aaccgggcgc ttcaaggtat atcgcgccat
    52321 tatgacccgc caggcgaaga aaggttgagt atctgatatc tgtcgccgca taccgtgtcg
    52381 gatgcggcgt gaacgcctta tccgacctac aaaacctgca cgttagccct tcgtaggcca
    52441 gataagacgc gccagcgtcg catctggcat ctgcccccgc atgcctacca aacctgcacg
    52501 ctagtttcct gatggacatt tttccagcaa ttacacatca ggcgataatt aactattgac
    52561 gaaaagctga aaaccactag aatgcgcctc cgtggtagca attcttttta agaattgatg
    52621 gtatgcgaag gtggcggaat tggtagacgc gctagcttca ggtgttagtg tccttacgga
    52681 cgtgggggtt caagtccccc ccttcgcacc aaaaaccacg ttgatattgc tcgcactggg
    52741 cgaaggtggc ggaattggta gacgcgctag cttcaggtgt tagtgttctt acggacgtgg
    52801 gggttcaagt cccccccctc gcaccaaacg aggcgatatc aaaaaaagta agatgactgt
    52861 gcgaaggtgg cggaattggt agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg
    52921 ggggttcaag tcccccccct cgcaccaatt atctttactt cctttcttgt ttcttccctg
    52981 ttttgtcatc cctgattcaa attcattatc atcagcatga tgccagcaat aagcgccagg
    53041 cacgatggta gcaaaatgta atgccgcaac tgtttgtgat acagcatcac cggcgtcaat
    53101 actaatccca tcagaatgac aatccgccac tgatccggcg ataaatccgc atacatcagc
    53161 cctgcaatca gcaatcccgg cagcaacact ccccagccac tgcccagcgt acgttgcaac
    53221 atggtttcat ctctttcatt gataatgaaa accaatatca tatgataatt tttatcattt
    53281 gcaagccaga gaaatccctt gctatcgagt aaacctatcg ataggattag caatcattat
    53341 catttagatt actatcacga ttatggccta tcgttccgca ccgctctatg aagatgtcat
    53401 ctggcgaacg catctccagc cgcaggatgc tgggcttgca caagccgtac gggcgatgat
    53461 cgccaaacat cgtgaacatt tgctggagtt tatccgcctg gatgaacccg ctccgcttaa
    53521 cgccatgacg ctggcgcaat ggtcatcacc gaatgcgcta agttctctac tggcggtcta
    53581 ttccgatcat atctatcgca accaaccaac gatgatccgc gagaacaaac cgctgatctc
    53641 actatgggca caatggtata tcggcctgat ggtgccacca ttaatgctgg cgctactgac
    53701 gcaggaaaaa gcattagatg tgtcgccgga acatttccat gctgagtttc acgaaaccgg
    53761 acgcgtcgcc tgtttctggg tcgatgtgtg tgaagataaa aacgcaacac cacattcgcc
    53821 gcagcagcga atggaaacgt taatcagcca ggcgctggtt ccggttgtgc aagcactaga
    53881 agcgaccgga gagatcaacg gcaaacttat ctggagtaat accggttatt tgatcaactg
    53941 gtatctcact gagatgaagc aactgctcgg cgaggctact gttgaatcgc tgcgccatgc
    54001 cctctttttt gagaaaacgc tcacgaacgg tgaagataat ccactctggc gtaccgtggt
    54061 actgcgcgac ggcctgctgg tgcgccgcac ttgctgccag cgttatcgct taccggatgt
    54121 gcagcaatgt ggctattgta cgctgaaatg aaagcactgc cggggaagta aacccggcat
    54181 catgcggatt aatagggatg caacacatta cttgcttcgc aatgccgcat acataacaga
    54241 atatctgcgg cctccaacaa ccctgcatca gaactgacgc ccagtttcga catcacatta
    54301 aatttatgcg ctcggatcgt tttgatatta cgtttaagct gctcggcaat ttgtgtcatt
    54361 gagtagccac gcgacataaa gcgcaatatt tctctctccg tcgggcttaa cgtccggctt
    54421 tggttaatgt accactgatt gttcagtcgg tcggtcgcct gacgtacacc atttaatgac
    54481 aaaaagaatt cctgatgaaa aatctccagc gttgacgctt tacttaatac accgtccagc
    54541 ggtgatggcg acaatgaacc aatcagccga gcttcaatat catcatccac aataactaaa
    54601 cgccgggtac gcggaaactt aatcgccagc tcagtcaggc aagataatcc ctctctccgc
    54661 tcacttctca tggcagaaaa agaaaaaatg accgccgcaa acgagatccg cgacatggca
    54721 tcctgaaatg actcctgact ggtataggta tggagcttat aagggttgcc cgcaaacttt
    54781 ctgaataaac tctcaatacc gatactactc atgacgcatt tttctatgag tgcgacattt
    54841 ctcttcttaa ttcggctgtg ttccattctg cggcatctcc ataaacagaa ggcaagttaa
    54901 gttctcttgc accctgcgcg ctatttatcc atgcatacat ttcagcgttg ctgtgaattg
    54961 ccagtcgacg catcgcactg tttttttggg cgctgatagt tttattactt ttcttaagca
    55021 atgaagctat ctggttgatg ccccatcctt tacctaatag ccgcagtacc ttgcgctctg
    55081 aaagagtgag aatgacggag cgatagctaa aatcatgagt ttcagtcatt gccggagagg
    55141 tcagcatggc gctgatacgc tctgcatgcg tattgccgga acgtacggtc ttgaccagat
    55201 tctcaatggg ttcaacatca gacaataacg tcgccgtagg gcacatgagc agttctacag
    55261 cctgggaata ccaggagcgt gacaccatga aaacccagtg aatttcccga tattgtgaga
    55321 ttaaagaata ataatgttca caaacagaac gtggatcttc actttcaccg gctaaatcag
    55381 caatgac
//
LOCUS       DAJTNM010000014        53212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTNM010000014 DAJTNM010000000
VERSION     DAJTNM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.014"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(12..323)
                     /locus_tag="OO172_000945"
     CDS             complement(12..323)
                     /locus_tag="OO172_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5511220.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            486..582
                     /gene="ffs"
                     /locus_tag="OO172_000946"
     ncRNA           486..582
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO172_000946"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            702..1055
                     /locus_tag="OO172_000947"
     CDS             702..1055
                     /locus_tag="OO172_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5511221.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="OO172_000948"
     CDS             complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="OO172_000948"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5511222.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLHQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2811..3281)
                     /locus_tag="OO172_000949"
     CDS             complement(2811..3281)
                     /locus_tag="OO172_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5511223.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3397..3948)
                     /gene="maa"
                     /locus_tag="OO172_000950"
     CDS             complement(3397..3948)
                     /gene="maa"
                     /locus_tag="OO172_000950"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR5511224.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4120..4338)
                     /gene="hha"
                     /locus_tag="OO172_000951"
     CDS             complement(4120..4338)
                     /gene="hha"
                     /locus_tag="OO172_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5511225.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="OO172_000952"
     CDS             complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="OO172_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5511226.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5284..8433)
                     /locus_tag="OO172_000953"
     CDS             complement(5284..8433)
                     /locus_tag="OO172_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5511227.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="OO172_000954"
     CDS             complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="OO172_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5511228.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9791..10438
                     /gene="acrR"
                     /locus_tag="OO172_000955"
     CDS             9791..10438
                     /gene="acrR"
                     /locus_tag="OO172_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5511229.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10566..13928
                     /gene="mscK"
                     /locus_tag="OO172_000956"
     CDS             10566..13928
                     /gene="mscK"
                     /locus_tag="OO172_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5511230.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="OO172_000957"
     CDS             complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="OO172_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5511231.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14315..14842)
                     /gene="priC"
                     /locus_tag="OO172_000958"
     CDS             complement(14315..14842)
                     /gene="priC"
                     /locus_tag="OO172_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5511232.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14912..15289
                     /locus_tag="OO172_000959"
     CDS             14912..15289
                     /locus_tag="OO172_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5511233.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15442..15993
                     /gene="apt"
                     /locus_tag="OO172_000960"
     CDS             15442..15993
                     /gene="apt"
                     /locus_tag="OO172_000960"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5511234.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16122..18047
                     /gene="dnaX"
                     /locus_tag="OO172_000961"
     CDS             16122..18047
                     /gene="dnaX"
                     /locus_tag="OO172_000961"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5511235.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17380..17444
                     /gene="dnaX"
                     /locus_tag="OO172_000961"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18100..18429
                     /locus_tag="OO172_000962"
     CDS             18100..18429
                     /locus_tag="OO172_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5511236.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18429..19034
                     /gene="recR"
                     /locus_tag="OO172_000963"
     CDS             18429..19034
                     /gene="recR"
                     /locus_tag="OO172_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5511237.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19144..21018
                     /gene="htpG"
                     /locus_tag="OO172_000964"
     CDS             19144..21018
                     /gene="htpG"
                     /locus_tag="OO172_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5511238.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21199..21843
                     /gene="adk"
                     /locus_tag="OO172_000965"
     CDS             21199..21843
                     /gene="adk"
                     /locus_tag="OO172_000965"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5511239.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21975..22937
                     /gene="hemH"
                     /locus_tag="OO172_000966"
     CDS             21975..22937
                     /gene="hemH"
                     /locus_tag="OO172_000966"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5511240.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22934..23894)
                     /gene="aes"
                     /locus_tag="OO172_000967"
                     /pseudo
     CDS             complement(22934..23894)
                     /gene="aes"
                     /locus_tag="OO172_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24046..25350
                     /gene="gsk"
                     /locus_tag="OO172_000968"
     CDS             24046..25350
                     /gene="gsk"
                     /locus_tag="OO172_000968"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5511241.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25483..27159)
                     /locus_tag="OO172_000969"
     CDS             complement(25483..27159)
                     /locus_tag="OO172_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5511242.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGKIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27397..28617)
                     /gene="fsr"
                     /locus_tag="OO172_000970"
     CDS             complement(27397..28617)
                     /gene="fsr"
                     /locus_tag="OO172_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5511243.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSQGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28835..30487
                     /gene="ushA"
                     /locus_tag="OO172_000971"
     CDS             28835..30487
                     /gene="ushA"
                     /locus_tag="OO172_000971"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5511244.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30524..31003)
                     /gene="ybaK"
                     /locus_tag="OO172_000972"
     CDS             complement(30524..31003)
                     /gene="ybaK"
                     /locus_tag="OO172_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5511245.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31207..32001)
                     /locus_tag="OO172_000973"
     CDS             complement(31207..32001)
                     /locus_tag="OO172_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5511246.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32139..32480
                     /locus_tag="OO172_000974"
     CDS             32139..32480
                     /locus_tag="OO172_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5511247.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32695..35199)
                     /gene="copA"
                     /locus_tag="OO172_000975"
     CDS             complement(32695..35199)
                     /gene="copA"
                     /locus_tag="OO172_000975"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5511248.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35461..36393
                     /gene="glsA"
                     /locus_tag="OO172_000976"
     CDS             35461..36393
                     /gene="glsA"
                     /locus_tag="OO172_000976"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5511249.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36396..37688
                     /locus_tag="OO172_000977"
     CDS             36396..37688
                     /locus_tag="OO172_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5511250.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37813..38220
                     /gene="cueR"
                     /locus_tag="OO172_000978"
     CDS             37813..38220
                     /gene="cueR"
                     /locus_tag="OO172_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5511251.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38221..38679)
                     /locus_tag="OO172_000979"
     CDS             complement(38221..38679)
                     /locus_tag="OO172_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5511252.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38676..39593)
                     /locus_tag="OO172_000980"
     CDS             complement(38676..39593)
                     /locus_tag="OO172_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5511253.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39739..40416
                     /gene="fetA"
                     /locus_tag="OO172_000981"
     CDS             39739..40416
                     /gene="fetA"
                     /locus_tag="OO172_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5511254.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40403..41182
                     /gene="fetB"
                     /locus_tag="OO172_000982"
     CDS             40403..41182
                     /gene="fetB"
                     /locus_tag="OO172_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5511255.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41245..42099)
                     /gene="cnoX"
                     /locus_tag="OO172_000983"
     CDS             complement(41245..42099)
                     /gene="cnoX"
                     /locus_tag="OO172_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5511256.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42160..42969)
                     /gene="ybbO"
                     /locus_tag="OO172_000984"
     CDS             complement(42160..42969)
                     /gene="ybbO"
                     /locus_tag="OO172_000984"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5511257.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42959..43581)
                     /gene="tesA"
                     /locus_tag="OO172_000985"
                     /pseudo
     CDS             complement(42959..43581)
                     /gene="tesA"
                     /locus_tag="OO172_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43552..44238
                     /locus_tag="OO172_000986"
     CDS             43552..44238
                     /locus_tag="OO172_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5511258.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44235..46649
                     /locus_tag="OO172_000987"
     CDS             44235..46649
                     /locus_tag="OO172_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5511259.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO172_000988"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO172_000988"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5511260.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OO172_000989"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OO172_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5511261.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="OO172_000990"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="OO172_000990"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5511262.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="OO172_000991"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="OO172_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5511263.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="OO172_000992"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="OO172_000992"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5511264.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALNSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53212
                     /locus_tag="OO172_000993"
     CDS             52939..>53212
                     /locus_tag="OO172_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5511265.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12821 a        13708 c        14019 g        12664 t
ORIGIN      
        1 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat
       61 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc
      121 gaaattgtgc catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
      181 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
      241 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
      301 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
      361 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
      421 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg
      481 ggctctgttg gttctcccgc aacgctactc tgtttaccag gtcaggtccg gaaggaagca
      541 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
      601 tcccaccgtt tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca
      661 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
      721 ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
      781 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac
      841 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
      901 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga
      961 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     1021 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
     1081 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
     1141 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggtgt agccactctt
     1201 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
     1261 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     1321 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat
     1381 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     1441 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     1501 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     1561 caagattgat cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa
     1621 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     1681 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     1741 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     1801 cacgattttc gatagcatcc tgcagccgat gatgcggtga ctggatacgg cgcaaaatac
     1861 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     1921 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     1981 tcatctccgg gaagggcagg atatcgtaaa tgatcccatt attttcgata tgctcaccgg
     2041 gtgttttttg tagcctggta ataattccct gagcaatttc atcgctgctg gttatgacaa
     2101 cgttatgggc attgccaata atggcggcat caatttgcca tgagctatag ggaatgacat
     2161 caataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     2221 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     2281 ttttacctgg ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg
     2341 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
     2401 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca
     2461 gcgcatcttt cccttgtgtc gccaccttat tggctcgaat agcgacgcgg gaggaatagg
     2521 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
     2581 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
     2641 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
     2701 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
     2761 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
     2821 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
     2881 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
     2941 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
     3001 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
     3061 ttaagcagga taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc
     3121 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
     3181 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
     3241 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
     3301 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
     3361 ctaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
     3421 tggattaccg cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc
     3481 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
     3541 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
     3601 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
     3661 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
     3721 gagaaaaatg ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac
     3781 ctgaccgaat aaatcagcga gaatttgctg gcgtaatgtg tgctcttccg ctagggaatg
     3841 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
     3901 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
     3961 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta
     4021 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
     4081 aataaacagc cggttatagc tccgaaagcg aattatttat tagcgaataa atttccatac
     4141 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     4201 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     4261 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt
     4321 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     4381 caggattctc tttcgtcgca ttgacaaaac aacggaatag tcgattacct gacttccgcc
     4441 atttctgaag agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca
     4501 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     4561 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     4621 ggtcgttaac ccagccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg
     4681 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     4741 cgcgtcccct tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     4801 acctagactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
     4861 cgcattatta cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac
     4921 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
     4981 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     5041 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
     5101 tattaaatct attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat
     5161 tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc
     5221 ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg attacatgtt
     5281 gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc ggctaaagcg
     5341 gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac
     5401 catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact
     5461 gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga tcggacgtaa
     5521 acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa
     5581 gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa tggttgtgag
     5641 cagtcctact tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc
     5701 accgataacc cccagcggaa cgaccagcat aacggagaac ggagtcgacc agctctcgta
     5761 cagcgccgcc agacacagga atacgacaat caacgaaacc gcgtacagtg aaggtgcctg
     5821 gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa caccggtagg
     5881 cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt tacccggtgc
     5941 cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac gcggcgaacc
     6001 gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat cagcagcacg
     6061 aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg acatgacgta
     6121 aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc
     6181 gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc
     6241 gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcagcat
     6301 atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca gtttttcgtg
     6361 accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac ccagttccac
     6421 gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt
     6481 tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccagtcctt
     6541 caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac
     6601 ggcgaacacc gactcaacgt tgtttttttc tttggtcaga tagtaatgcg ttacctcatt
     6661 gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa
     6721 tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat
     6781 gccgaccacg atgatcagat acagcactag gtaacgcccc gtactgcgca gaataccgcc
     6841 tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttaaacc agccgaagaa
     6901 gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag
     6961 agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa caatggtaat
     7021 agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac
     7081 cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg
     7141 ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt tttctaccac
     7201 aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca ttgttagcgt
     7261 gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac
     7321 ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca taaccaggaa
     7381 cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa
     7441 cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt
     7501 cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg tcgccagctt
     7561 gatccccaga ccggaagccg gttggccgtt aaactctgcg atgatgtcgt agttctcacc
     7621 acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct gattcacttt
     7681 cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc
     7741 gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg caacctgggc
     7801 gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagctc
     7861 attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa catcacccac
     7921 gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag agatatcctc
     7981 ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga
     8041 tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca tcgccagctg
     8101 cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact caaaggtcag
     8161 ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat
     8221 accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc
     8281 gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgtgccac
     8341 cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc
     8401 aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag
     8461 acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt
     8521 cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat
     8581 cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg cttgcaacga
     8641 tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca tcaccacgcg
     8701 gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca
     8761 gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc
     8821 gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt
     8881 cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt
     8941 gtttcagcgt gccattcgcc agttcctgtt tcaggcgcag gaagtcgttg ctggactggg
     9001 tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt
     9061 tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta atcggagagg
     9121 tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc gccgcagtta
     9181 ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtacccttgc ttactgatgt
     9241 actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag
     9301 cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag gtcgcaggat
     9361 caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa ttacgcttca
     9421 ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg
     9481 gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca
     9541 tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg
     9601 agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc
     9661 gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat
     9721 attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat
     9781 tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct
     9841 cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggcgagat
     9901 tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga
     9961 tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag agcttgagta
    10021 tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct
    10081 tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat tccacaaatg
    10141 cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag
    10201 ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga
    10261 tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc tgatggaaaa
    10321 ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt acgtcgccat
    10381 cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgctacta acgaataacc
    10441 ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct ggttcactgc
    10501 gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat
    10561 tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta
    10621 ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg tcatcgaatg
    10681 gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat aaacaaaaag
    10741 atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg
    10801 ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc
    10861 cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc gataacgacg
    10921 aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact cgcgttgccc
    10981 aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac agccagctgg
    11041 tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct tcgcagcagc
    11101 tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca
    11161 gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt gaccagcagc
    11221 gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa cgtgattacg
    11281 tgacggcgaa cagcgcccgt ctggagcatc agttgcagct gttgcaagaa gcggtaaaca
    11341 gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg gatgaagccg
    11401 cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag cagttaagtc
    11461 agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac attaaagtca
    11521 aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag attgccgtcc
    11581 tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg ctgccctcgg
    11641 cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag
    11701 ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa ctggaagaag
    11761 gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg gttgatatgc
    11821 gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg atggccatta
    11881 acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa tccatcctga
    11941 ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg atcaaagcgt
    12001 tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag
    12061 cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg attgccgggc
    12121 tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct tccgctgtgg
    12181 gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc gacctgatcc
    12241 gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg accatgcagc
    12301 tcaatatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc tggctggtgt
    12361 ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac ttcggcatgc
    12421 cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc gcgttgctgc
    12481 ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg gatgatgtgc
    12541 tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta tggccaatgt
    12601 gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actagtcact attaccgtgc
    12661 tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc tacaccacgc
    12721 tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg aacttgctct
    12781 accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcgc
    12841 tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac
    12901 ccaccattgc actggagcaa gttaaccagc agacgctgcg tattaccatg ttgctgatgt
    12961 ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc gtgttcagct
    13021 atctcgacag catcacgctc tggcattaca acggcactga agctggcgct gcggtggtga
    13081 aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt
    13141 tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg aatatgcgcc
    13201 agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga
    13261 tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg gccgcagcat
    13321 tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc tccggtttga
    13381 tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt agcttctcgg
    13441 ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag
    13501 tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca
    13561 ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg gaaaaagtgc
    13621 gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa ccaatgccgg
    13681 aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc
    13741 gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc gatcagctgt
    13801 gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga
    13861 agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg acaccagcgg
    13921 ttgggtaata accaaagggg gcgacattta gttgccccga gattactgac aaagtgcgcg
    13981 ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc aagaattcaa
    14041 tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc
    14101 ccgcaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
    14161 gtgcgttaat ttcttttcat aatcacgctt tacaatatcc agcgcgcgca gcacggtact
    14221 ggcagggatc tgattttcct ccagcagcac aatcaaatcg acagccagtt tgacatcgtc
    14281 aggggcattt tcaagtgaca tactctttcc attgctagcg ggttaagcgc gctagcctgt
    14341 tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccactt
    14401 cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg gctctgcgtt
    14461 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
    14521 agcgggcaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gcggcttcac
    14581 gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact tgcggcagct
    14641 gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctcg tcgagacaag
    14701 cctgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca cttagcgtgg
    14761 cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct tccagttttt
    14821 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
    14881 gtagccactc tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
    14941 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
    15001 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
    15061 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
    15121 gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca tttctctgtg
    15181 gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
    15241 ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
    15301 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
    15361 cggtcaaagg ttaaacaacg gttacttttg atacgtttaa aacgcgccgt gagtaccacc
    15421 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    15481 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    15541 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    15601 aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    15661 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    15721 gaaaccatca gtgaaaatta cgacctggaa tacggcaccg atcagctgga aatccacgtt
    15781 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
    15841 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg
    15901 ttcattatca acctgttcga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
    15961 agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg ctgcccacgc
    16021 tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    16081 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggttttag
    16141 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    16201 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
    16261 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
    16321 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    16381 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat acccgcgacc
    16441 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctgatcgacg
    16501 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt gaagagccgc
    16561 cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg gtgacgattt
    16621 tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcagc
    16681 ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc gcgggcgctg caattgttgg
    16741 cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    16801 gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg cttgacgacg
    16861 atcaggcgct gtcgctggtt gaagcgatgg tcgagggcaa cggcgagcgc gtaatggcac
    16921 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    16981 gcttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    17041 ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    17101 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgca
    17161 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg ccgctgcctg
    17221 agccagaagt gccacgccag tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa
    17281 ccctggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    17341 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    17401 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    17461 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
    17521 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg cagcaaaaag
    17581 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    17641 tggcggcgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca caggtgagcc
    17701 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
    17761 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggcgcac
    17821 agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa ctgactatcg
    17881 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
    17941 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    18001 ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    18061 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    18121 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    18181 atcgcgcagc tggaagttac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    18241 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    18301 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    18361 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    18421 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    18481 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    18541 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    18601 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
    18661 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc attgagcaga
    18721 cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg ctggacggca
    18781 tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    18841 ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct aactacattg
    18901 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    18961 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctccctcgcc gggcgtcata
    19021 agattcgttt ttaagcaaac gagagcagga gcacctgctc tcgcttgaaa ttattctccc
    19081 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    19141 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    19201 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    19261 tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct gtacgaaggt
    19321 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    19381 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    19441 tccggtacca aatcattcct cgaatccctg ggttctgacc aggcgaaaga cagccagctg
    19501 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
    19561 cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa
    19621 ggtgaatata ccgttgccga catcaccaaa gaagatcgtg gtactgaaat caccctgcat
    19681 ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat catcagcaaa
    19741 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agatggcgaa
    19801 accgttatct cctgggcaaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    19861 gaaatcaccg atgaagagta taaagagttc tacaaacaca tcgcccacga ctttaacgat
    19921 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    19981 atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg
    20041 tacgttcaac gcgtgtttat catggacgac gctgaacagt tcatgccgaa ctatctgcgc
    20101 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    20161 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    20221 ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca acagtttggc
    20281 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    20341 ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct ggaagactac
    20401 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga cagctatgcg
    20461 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    20521 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    20581 ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    20641 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgcgtgaa agctctgctc
    20701 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    20761 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    20821 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    20881 gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct gctggatcag
    20941 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaac
    21001 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    21061 tcatcatctg tactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt
    21121 tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa atcgacacat
    21181 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    21241 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    21301 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    21361 gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    21421 gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    21481 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    21541 ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac
    21601 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact
    21661 acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg
    21721 actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg
    21781 aaagttgacg gtaccaagcc ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc
    21841 taatcaagcc gcagcagatc tgctttcggg caggtctgct catccacctc aggcaatacc
    21901 tctcacagca attagttctt cttcctcact tttccgctac aattatcaac aagttgaatc
    21961 gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac ctgggtacgc
    22021 ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac
    22081 gcgtggttga tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg attttgccgc
    22141 tgcgctcgcc gcgtgtggcg aagctgtacg cctctgtctg gatggaagac ggctcgccgc
    22201 tgatggttta cagccgccag caacagcagg cgttggcaca acgtttaccg gatacgccag
    22261 tggcgctggg catgagctac ggctcgccat cactggaaag cgccgtcgat gaactcctgg
    22321 cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttctacgg
    22381 tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga
    22441 tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg gcgaacagcg
    22501 tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct tatcatggca
    22561 ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc acaacgactc
    22621 gcgaactggc ttccgcactg gggatggtac cggaaaaagt gatgatgacc tttcagtcgc
    22681 gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag
    22741 aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat tgtctggaga
    22801 cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc gggaaaaaat
    22861 atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg gctaatcttg
    22921 ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt
    22981 cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct gggtagagtt
    23041 tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg ctgtcatcca
    23101 gcagcggatc gaactcctcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat
    23161 tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt
    23221 catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag
    23281 tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca attttaccgc
    23341 aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg gcacctgcgg
    23401 aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc tgctggtgga
    23461 aataacaaca agcagccaca atttcctcta tcgcttgtgg aaaacgcgct tcaggtgaaa
    23521 gggtgtaatc aataccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc
    23581 gatcgtgggt atcgagattg ccgagaataa aaccgccttc catgcaaata aaatagcgtc
    23641 gccgggctat ctggctgcgg acaaaagaga cgtgtttcca cctgcccata ttttgttgga
    23701 accatgtaag ctctggttgc catttctgga gcgcccgcat tccagaatcg gcgctcaagc
    23761 gtgtaatact gtcgttgctc agcaatcgtt cccgttgcgg gccaggacgg taaatccggc
    23821 tgaagagtat tcacaacggt cttcatttca gcagaaataa ggtccagaac aggtagtttg
    23881 ttttccggct tcatacaaaa actcctttca aattacgtca ttgtaaggaa ccactgccat
    23941 gaaaatgcga tcccgcctgc tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga
    24001 ttgtgttaac attcgccgct cagttaacca cccgtaaaaa caaccatgaa atttcccggt
    24061 aaacgtaaat ccaaaaatta cttccctgta aatgcacgcg atccgctgct tcagcaattc
    24121 cagccagaaa acgaaaccag cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat
    24181 attgaagcga aagtggatga tgaattcatt gagcgttatg gattaagcgc cgggcattca
    24241 ctggtgattg aggatgacgt agccgaagtg ctttatcagg agctaaaaca gaaaaacctg
    24301 attacccatc agtttgcggg tggcaccatt ggtaacacca tgcacaacta ctcggtgctc
    24361 gcggacgacc gttcggtgct gctgggcgtc atgtgcagca atattgaaat tggcagttat
    24421 gcctatcgtt acctgtgtaa cacttccagc cgtaccgatc ttaactatct acaaggcgtg
    24481 gatggtccga ttggtcgttg ctttacgctg attggcgagt ccggggaacg tacctttgct
    24541 atcagtccag gccacatgaa ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc
    24601 ggagcctcgg cactggttct cacctcatat ctggtgcgtt gcaagccggg tgaacccatg
    24661 ccggaagcaa ccatgaaagc cattgagtac gcgaagaaat ataacgtacc ggtggtgctg
    24721 acgctgggca ccaagtttgt cattgccgag aatccgcagt ggtggcagca attcctcaaa
    24781 gaccacgttt ctatccttgc gatgaacgaa gatgaagccg aagcgttgac cggagaaagc
    24841 gatccgttgt tggcatctga caaggcgctg gactgggtag atctggtgct gtgcaccgcc
    24901 gggccaatcg gcttgtatat ggcgggcttt accgaagacg aagcgaaacg taaaacccag
    24961 catccgctgc tgccgggcgc tatagcggaa ttcaaccagt atgagtttag ccgcgccatg
    25021 cgccacaagg attgccagaa tccgctgcgt gtctattcgc acattgcgcc gtacatgggc
    25081 gggccggaaa aaatcatgaa cactaatgga gcgggggatg gcgcattggc agcgttgctg
    25141 catgacatta ccgccaacag ctaccatcgt agcaacgtac caaactccag caaacataaa
    25201 ttcacctggt taacttattc atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc
    25261 tatcaggtac tgaaccagca ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac
    25321 agcctggaag agtcttactg ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc
    25381 gttcacgccg catctggcat ttggctctcg atgcctgatg cgacgctggc gcgtcttatc
    25441 atgcctacat atttttcata ttttacatcc ggcaaccacc gtttaccccg tcaccacttc
    25501 acccgccggt ggcgtttcca gcagttccag catagtacgg gcaatttcac gttcacccat
    25561 cactacctga ttcgcgccac gttcggtgat atacgtcact tcatcgtcat aatgggcgcg
    25621 ggcgataatc tcaatatccg ggtttttcgc gcgggcagac gccacaatct cacccgcttc
    25681 ataaccgttg gggatcgtca ggatcagcca ttttgcacat tccagatgtg ccagttgcat
    25741 aatttcttcg ttcgctgcat tgcccaatac agcgcggacc ccacgctctc gcagctcatc
    25801 aacacgggtt cgtgacgtct caatcaccac cagcggaata tcagaggcga gcaatttctc
    25861 ccccagcagg ctgcctacac gaccgtaacc caccaatagc gcatggttgc aaatatccac
    25921 tgggatctgt ttctcttctt cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt
    25981 cttcgccaga tatttctcca gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat
    26041 cgcccctgcc agtaccaggt tttgtccggc ctgcggcagt aaattcaatg ccattcccag
    26101 tcccgccagg ataaacgcga actcaccaat ctgcgccagg ctggcggcga tggttaatgc
    26161 cgtacgttgg gagtgaccaa acagacggac aaggaacagg gccgcgaccg atttaccaaa
    26221 cagaataatt gccagcgtcg ccagcactgc cagcggttgc tgaatcagaa ttaacggatc
    26281 aaacaacatc ccgactgaga caaaaaacag caccgcaaac gcgtcgcgca atggcaacgt
    26341 atcgtgggcg gcacggtgac tcaattcaga ctcgttcagt accatcccgg caaagaacgc
    26401 accgagtgca aaggagacat caaacagctc taccgcacca aaggcaatcc ctaacgccag
    26461 cgccagcact gatagggtaa acagttcgcg agaaccggtt gccgcgctgc gtgccataat
    26521 ccacggcacc agacggcgac ctaccagcat cataatggcg ataaatgcga tcaccttgcc
    26581 gatggtgatc cccatatcga ctgcaagagt ggcaaagccc acatcgccct gttccatcat
    26641 tcctgccact gcgggcagca acaccagcgt cagaaccatc accaggtctt ccacaatcaa
    26701 ccaaccgatg gcgattttcc cacgctgact gtctattaat tgccgttctt caagtgcgcg
    26761 cagtaacacc acggtactgg cggtggaaag acataagccg aacacgatac cggtcattaa
    26821 cgaccagccc agcacggcgg agagcgccat acccagcagc gtcgccacgg ctatctgggc
    26881 gatcgcgccg ggaatggcga tggactttac cgccatcaaa tccttgagcg aaaaatgcaa
    26941 accgacgccg aacatcagca aaataacgcc cagttcagcc agttccggcg caagcttggt
    27001 atcggcaaca aagcccggag tgaatggtcc tgccagcaca cccgctaaca gatatcccac
    27061 cagaggagaa atacgtagtt tattggccag catgccgagg ataaaggcga gcacaaggcc
    27121 gccaacaatg gtggtgataa gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg
    27181 ttattgtcca tttttggccg ggaaaaccaa aattacaggt aatagtttat gacaatttca
    27241 ttgatgatgt ttatgaataa ttgttgaatt ttgcagaaaa atggaattag ctgcaaaaaa
    27301 agcacggatc ggaaaacaga aggcggttta acagaggaaa ggcttatggc gactgtaaaa
    27361 cgatgcagcc aaagtttgcc tttggctgca atgaaatcag tctttatgcc ggttatcagg
    27421 caggaatatg gtcaacatcc ccaatagtgg caggaaagca cagattttat agactaactc
    27481 gatgctggtg tgatcggcga taagccccag aactgccgct cccagacctc ccatgccgaa
    27541 agcaaaaccg aaaaagagtc cagaaatcat accgatacgt cccggaagta gctcctgagc
    27601 gtagaccaga atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac
    27661 ccccgtccag tgcaggcggg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat
    27721 agagccccaa atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt
    27781 acctgccgca acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa
    27841 tccgaatttt tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata
    27901 tttcgagaaa atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag
    27961 tggattgatt atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca
    28021 acggctaatt tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac
    28081 gttgcctttg ccataaggcg cgataatcac cgccgccagc aaggggccct gggaactgcc
    28141 aaagttgccg ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat
    28201 acgggctacg cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc
    28261 cgccagcaga actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt
    28321 aaagcacatg ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac
    28381 cactggttgc agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat
    28441 aaatgtcaga gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat
    28501 catgtcgttc agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc
    28561 cttggtcgtt gacgcagccg cgcccgccac aggctgggtt tgttcactca ttgccatagg
    28621 aaagtcactt tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac
    28681 taacatacct gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc
    28741 tggatgcaga tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt
    28801 tttaacacgt tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc
    28861 gttagcgctg ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga
    28921 taaaacctac aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa
    28981 tgaatatggc gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga
    29041 ggttgcggct gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt
    29101 gcccgagtct gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta
    29161 tgacgcgatg gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca
    29221 ggaaaagtgg gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga
    29281 gcgcctgttt aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg
    29341 gctgacaacc gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt
    29401 tcgtaagccc gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc
    29461 agacattatt atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa
    29521 cgcaccgggc gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt
    29581 cggtggtcac tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta
    29641 cgtgccgggt acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca
    29701 tgagtggggc aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat
    29761 ggttaactac cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa
    29821 aagcgagcgc gtgctttaca ctcctgaaat cgctgaaaac cagcaaatga tctcgctgtt
    29881 atcaccgttc cagaacaaag gcaaagcgca gctggaagtg aaaataggca aaaccaatgg
    29941 tcgtctggaa ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat
    30001 tctggcagcc caaatggatc gcactggtgc cgactttgcg gtgatgagcg gaggcggaat
    30061 tcgtgattct atcgaagcag gcgatatcag ctataaaaac gtgctgaaag tgcagccatt
    30121 cggcaatgtg gtggtgtatg ccgacatgac cggtaaagag gtgattgatt acctgaccgc
    30181 cgtcgcgcag atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt
    30241 ggcgaaagac ggcaaactga acgaccttaa aatcaaaggc gaaccggtcg atccggcgaa
    30301 aacttaccgt atggcgacat taaacttcaa tgccaccggc ggtgatggat atccgcgcct
    30361 tgataacaaa ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta
    30421 tatccagaaa agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg
    30481 gcagtaatcc gaaagtgccg gatgtttgca tccggcacaa tgcttaatcg cggcgggcga
    30541 tatcagcaaa tttggcatcg aggatctttg ccagatcgcc tgccgccagt tcgatatcca
    30601 gtccgcgctt gccgccggaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa
    30661 tcgtgggcag acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg
    30721 aacgctgcgc gaccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta
    30781 cttttttaag atccagttga cctgcgaccg gcgtaacggc cacggcaagg tgtttcatat
    30841 caccgttcac tgccaccagc agcgttttgt agacctgatc cggattcaaa cctaattttt
    30901 tgacgacttc atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa
    30961 acgaaatctt gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt
    31021 aaacaaaaaa acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc
    31081 ctaagagtat tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag
    31141 tgacggcata ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc
    31201 atttttttag cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac
    31261 cgcgaccaca tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg
    31321 ctgatgcatc agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt
    31381 attttgcggc ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt
    31441 atgccagtgg gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa
    31501 catggcaatc tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc
    31561 cgcctgcaat agctggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg
    31621 cgtcgcctga agaaccatcg cgatttgcca cagcggttgg gtagaaaaga gtgagggaga
    31681 aatgcccatc tcctggctaa tgtgctgcag gttttggagt tgctcctcgc taatgcgctc
    31741 ttccagcgcc tcgcaggcag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc
    31801 ctcgacgatc agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc
    31861 catatcgtgg ctacccatat gaatactgcc aatcagatga aaatggcgat tgccggggag
    31921 ggtgatatcg atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt
    31981 tacccggtac aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg
    32041 agggatggcg caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc
    32101 ttgcatcgct attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac
    32161 cgactacacc cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca
    32221 atgagttagc agagttgctg catgttcatc gtaatcgcgt cagtgcactg atcaataaca
    32281 atcgtaaact caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg
    32341 attttaggct aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca
    32401 cccaggaaga attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc
    32461 gtgcaaaaaa ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt
    32521 tcacgccgca tccggcatcc gagcaccatt gcctgatgcg acgcttacgc gtcttatcag
    32581 gcctacaaac ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt
    32641 catgcgtcga tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc
    32701 cttcggttta aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc
    32761 cattgccgct ccggcaacta ccgggttaag cagtgttcca gtaaacggcc acaaaatacc
    32821 ggcggcgacc ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat
    32881 gttgcgcagc gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg
    32941 catcagggta atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc
    33001 gacatccgcc tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg
    33061 acgtccttca ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc
    33121 aatcacttca tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc
    33181 ggtcaacatc accagacgat atcccgcttt atgcaggcgt tgcagcgccg ccacgctatc
    33241 actacgcaac ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag
    33301 cacaggcgtt gctccttgcg atgcctgagc tgaaatatcg gcttcaatcg ctttagtatc
    33361 gacttgctga tcatttaaca acgcctgatt acccagcaat aacgcatgac cttctgcttc
    33421 accgctcacg cccagcccgc gcaatgtgcg gaaaccgttg acctgcggta gctgcatatc
    33481 gcctgcttta tcaaggatcg ctcgtgccaa tggatggctg gaaccttgct ccagcgccgc
    33541 cgccagacgc agcgcctgcg cttcgtcaac atcagcaaat gttttcactg cgacaacctg
    33601 cggcttccct tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc
    33661 gcgttgcagc gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc
    33721 ggaaataatc gacatcggcg tcgccagtcc cagcgcacac gggcaggcga taatcagcac
    33781 cgtagtggca atcaccaggg tatagacaat ctgcggtgcc ggaccaaaga aataccagat
    33841 tgccgcactg acaagcgcaa taaccactac taccggcaca aatacggctg agattttatc
    33901 cgccagctga ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatcct
    33961 tgatagcgtg gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac
    34021 cactgtcccg gcatggacgc tatcgccttc gcctttttgc tgcgggattg gttcgcccgt
    34081 cagcatcgct tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg
    34141 atcgccggtc gtcaggcgca gcaacatacc tggctgcact tctgccagag gcacgctttt
    34201 ttcaccttcg tcagtaacca ggcgcgccgt cggtggggtt aaatcgagta atttctccag
    34261 tgccttagaa gaacgctgac gtgcgcgtgc ttccagcata tggccgagat tgatcagacc
    34321 gataatcatc gcactggctt catagtaaag atgtcgcgct tccatcggga accactgcgg
    34381 ccacaggttg acgctcatcg aatagagcca cgccacgccc gttcccagcg ccaccagcgt
    34441 atccatcgtc gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc
    34501 ggcgaaaacc atcaccgcca gggttatcag gccgataacc aaccacaggc tgcggttgtc
    34561 ggcggtgacc atcatgttat cgccgatcat cccccagacc atcaccggga tacccaccgc
    34621 cagtgcgaca attgcctgcc agcggaagcg cttcatcgta gcgacggcgg tttcttgctg
    34681 gcgttcgcgg cgtttcgcgt catcttcaat cgcttccgcg ccgtagcccg ctttttccac
    34741 cgcctgcact aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag
    34801 gtttacccgt gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac
    34861 acagctggcg cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt
    34921 cgctgccgga agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc
    34981 cagcggttta gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc
    35041 gatcagctgt tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc
    35101 ttgttccaca tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca
    35161 ggacaggccg tccaggttca ggtcgatagt ttgtgacata aaacactcct ttaagacagt
    35221 tttgactggc tgtgataaag gttaaacctt ccagaaaggg gaaggtcaag aaattaataa
    35281 accaggcggg taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga
    35341 catttatttc tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct
    35401 gaaaatcggc acaggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag
    35461 atgttagatg caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca
    35521 cttaacggcg gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg
    35581 gcggcagtgg ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac
    35641 cgctttgcac tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc
    35701 ggcccgcagg cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca
    35761 gttatcgcct tagagttgca tggcggcaaa ccgctttcgc cactggtaaa tgctggcgct
    35821 attgccacca ccagcctgat taacgctgaa aacgccgaac aacgctggca gcgaatttta
    35881 catatccaac agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg
    35941 gaacaaacaa ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat
    36001 ctctattgtg atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc
    36061 aatactgttg aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg
    36121 acgcataaac gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg
    36181 gaagggctgt atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc
    36241 ggtgtaggtg gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca
    36301 ccaccgctgg acgaagaagg caatagtgtt cgcggtcaaa aaatggtggc atcggtcgct
    36361 aagcaactcg gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg
    36421 gtaacaaacc cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg
    36481 cggggatctt cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctggggcg
    36541 cctttgcttt tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg
    36601 cgagctatcc cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg
    36661 tcttttcgct agcgctctcg ttgctatacc tgttgacgct ggcggtgagc atcgccatgg
    36721 ttgcccgtgc ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc
    36781 accttatttt gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa
    36841 gcaaccatgc ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt
    36901 tattgctgat tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc
    36961 cccccagctc cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct
    37021 ttggcatgat ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg
    37081 cgtttctggt ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc
    37141 ttagcgatgt atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg
    37201 cttctccgct gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga
    37261 cggcttcagg cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca
    37321 gcgaacgcga actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca
    37381 acatcatcgt cgtggtgttg ataatgctga tgacggcggc gctaaattta gactcactcg
    37441 ccagcgttgc cagcgccacc tttttgatct gctatctggc ggtgtttgtg gtggcgatcc
    37501 gcctgcgtca tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt
    37561 tgttggtgat cgttggcttt atctacagcc tgtggtccca gggcagccgt gcgttgatat
    37621 ggattattgg tgcaatctta ctcagcctta ttgtggcaat ggtcatgaag cgtaataaaa
    37681 ccgtataaca cctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc
    37741 gcactggcaa aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc
    37801 gttgcgcgaa cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc
    37861 attcgcttct atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat
    37921 cgcacctaca cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg
    37981 ggctttaacc tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac
    38041 agcgccgacg tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag
    38101 gagctgcaat ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac
    38161 agcgccgact gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga
    38221 ttaagacgag acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacata
    38281 cgtacctgca ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat
    38341 atgaccgcgc ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg
    38401 cccgcgctgg tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca
    38461 tttccaccac agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc
    38521 ccaacccagc ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca
    38581 caacaaataa ccatttccgc ccagcatctc ggctgccagc agcaacccgc cgagactgag
    38641 ccagaaaata tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg
    38701 ctgtctttca ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat
    38761 ggcatcatca ctactttgct gttactggag gaaccgatct gctgtaacgc ttcggtgtat
    38821 ttttgcgcta cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc
    38881 attttggtgg cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac
    38941 gccgactgac gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg
    39001 aggatttccg cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc
    39061 gctttcatct gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt
    39121 tcaatacggg tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg
    39181 cgtgagttga tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg
    39241 gtacggatgt tagtcatggt caggttgatg atcgccagct ccagattgct gacttcatag
    39301 gcagcgcgtg gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg
    39361 ttatctttcg agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc
    39421 ttgcgaccaa tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg
    39481 gtatagcgac caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc
    39541 gcgccgacaa tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa
    39601 acctcctgtt gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct
    39661 gattactgaa agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat
    39721 cagaaaaagg gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga
    39781 tatctggcgg gtgatacgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa
    39841 tttaagttaa ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct
    39901 tcattgatca gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta
    39961 aaaccagaaa tctaccgcca gcaagtctct tactgcgccc agacgccgac gctgtttggc
    40021 gatacggtat acgataatct gatctttccc tggcagatcc gtgaccagca gcctgacccg
    40081 gccatttttc tcgattttct cgaacgcttc gctttgccgg acagcatttt gacgaagaat
    40141 atcgccgagc tatctggtgg tgaaaaacaa cgcatctcat tgattcgtaa cctacaattt
    40201 atgccgaagg ttttattgct ggatgaaata accagtgcgc tggatgaaag taataaacat
    40261 aacgtcaatg agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg
    40321 acacacgata aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc
    40381 ggagaaatgc aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact
    40441 ggcattaatg ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga
    40501 gaaagatatt ctctggagcg tcgggcgagc gataattcag ctgattattg tcggctatgt
    40561 gctgaagtat attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat
    40621 ctgctttaat gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat
    40681 ctcatcgttt attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc
    40741 agggtcgatt gaatttatcc cgatgcaggt gatccctgtc gccgggatga ttgccggtaa
    40801 cgccatggta gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca
    40861 gcaacagatt caggagaaac tgagtcttgg cgcgacgccg aagcaggctt cagcgatatt
    40921 gattcgcgac agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg
    40981 cttagtgagt ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa
    41041 ggcgattaaa tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac
    41101 cattattgcc tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt
    41161 gactcaattg aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg
    41221 gttcatgtgc cgaacaacgg aatatcaata caacaatgca tacagctggc ggcgatactt
    41281 cgacgccagt gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt
    41341 ctgaccgtct gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc
    41401 ttcattgcgc ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc
    41461 tggattctcc gccacctgct gttgcaactg ttgaatttcc ggcttatcag ccgcctgctt
    41521 cagcagttcg atttgcgcta ccagcccctg gtagcgggtg tcctgatcct gcaacggaat
    41581 ggttttcagc accgcttccg catcttcaga acggttcagc gcaatcagcg tttctgccag
    41641 cagcaggccg atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag
    41701 ggcatcggtg taattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc
    41761 ttcgcgcggc agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg
    41821 gaagccatct accggttgcc cgttctggaa cagatacacg gtcggaatcg cgcgcagacc
    41881 aaactgggcg gcaatcatct gctccgcgtc gcagtccagc ttcgccagaa taaattgccc
    41941 gttgtactgc gccgcgaggc tttccaggat cggggttaac tgcaaacagt gctggctacg
    42001 ttcagaccaa aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag
    42061 gttagattcg ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga
    42121 tattttcttt gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc
    42181 catcacgcgc ccaggcagca ggcgctcgag caccattact gcccaggtca ccagcgttac
    42241 cggatagcgc atcttcggct tctcgctaat aaaagcatgg cgcactttgt cgaccaccgc
    42301 ttccggtccc aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg
    42361 cgtctggttg acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac
    42421 tttaattccg ctgtggcgta gctccatgcg cagcgcgtct gaccacgcct ccagcgcata
    42481 tttactggcc gcgtaagcgc cacgacccgg cgtggagatt aatcccatca ccgatgatgt
    42541 catcacaata cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg
    42601 gtgtgcgccg aaaaagttgg cggaaaactg ctgttccatc tgcgcacggc tgatggtgga
    42661 aagggggcca tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt
    42721 cagggcgatc acctcgtctg ctgcgcgatt aacactttct ggtgaatcca gatcgatcaa
    42781 cacgccggta aatcccatgt tgttcatacg ctcaacatca ttcggttttc ggcaacctgc
    42841 cagcacatga aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga
    42901 acatccggta attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt
    42961 atgagtcatg atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct
    43021 gggcgtcgcg gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct
    43081 cttccataaa aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg
    43141 cgctaaaggc ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca
    43201 atggttcagc gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt
    43261 gctgtggctg aaaaccacgc aaaccatcat tgcgcccagt tcaaccagca cccaacgcgg
    43321 ctgatgctgt ttgagcagag ccggaaggcg cgccagccct tgttgcgagg tgtcgccgct
    43381 gatgctggca ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca
    43441 ggccgcgctg gcagacattc gatatccggc gctcaggcta tcacccagaa tcaataacgt
    43501 gtccgctgcg gcggcacgga aggttaacag gaccaggaac aggaagggca aatgccagcg
    43561 gaaaacattg ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc
    43621 tccatcctca ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc
    43681 gagtcgggat cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc
    43741 agtggcgaag tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca
    43801 aagttgcgcg cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt
    43861 aacgcgctgg aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt
    43921 cgtaacgggg cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt
    43981 ccggcacagc tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt
    44041 cgacctgatg tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat
    44101 aaaattgccg acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg
    44161 acccacgacc tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag
    44221 ttgcaggagg aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat
    44281 taattgtctg gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca
    44341 gcgatcgcat ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg
    44401 cgttgcgcag ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc
    44461 tgaaagtcgg caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc
    44521 tggcgaacgt caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc
    44581 cccctggcct gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc
    44641 ttaacctgaa aacggacgac accattgacg tgggcgatgc caccttgcgg attgccggag
    44701 aagtgattca ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga
    44761 tgaatctggc ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc
    44821 gttataaatt cggcggcaac gagaaccagc tcgacggcta tgagaaatgg ttgttacctc
    44881 agcttaaacc cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat
    44941 cgatggaacg ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag
    45001 tggcagcggt ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga
    45061 tcctcaaaac gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt
    45121 tgatggtgct gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg
    45181 tgttgatggt gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc
    45241 cgtggctgtg ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat
    45301 accgcttgtt gctggcaacg cagcctttac gcgtattacg taatgatgtg gtcgcgaacg
    45361 tctggccgct gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg
    45421 gattaatggg tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt
    45481 tgctgtgcgg tgtgctgggc tggatgctgc tgaatgtact tcgccgcatg acgctgaaat
    45541 cgctgcctct gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc
    45601 agctttcggc attttcgctc tcctttatgc tgctggcact gctgctggtg ctgcgtggcg
    45661 atctgctcga ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta
    45721 acatcgccac agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc
    45781 cggaatcatt ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag
    45841 aaggtaatga agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc
    45901 ccgatcataa tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg
    45961 aagaggggct ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg
    46021 atacccagga gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc
    46081 ggcctaattt ctattttatt ttccctgaag gggcattaga cgggcaaccg cagagctggc
    46141 ttaccagttt ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc
    46201 cgaccattag cctgttagac attggcgcga ttttaaaaca ggtcggtcag gtgctggagc
    46261 aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt
    46321 tgctggcaca ggtgcaggtg ggaatgcgcc agcgtcatca ggagctggtg gtatggcgca
    46381 cactcggtgc ggggaaaaaa ctgctgcgca cgacattgtg gtgtgagttc gccatgcttg
    46441 ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca gtgttgcagg
    46501 cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca
    46561 gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta
    46621 aggcgctgtt caggcagttt gtggggtgat gacaggtgtt agattgcctg atgcgctacg
    46681 cttatcaggc ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat
    46741 aaggcgttta cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca
    46801 actgtcatgt cggttttttt aaaggccgaa ggaggtaaac cttcggcctt agctacgttt
    46861 cctcagttat tcaataaact gacggatcag ttccagcagc gcgattagcg ccttaagaac
    46921 gatgagaact ttatcgagaa aagttttcat ccgttgctcc cttaagggag aaagacaatc
    46981 acagcttacc cttacgttgc ctgtaactat gattttcttc atgtgctaga tgaggactgc
    47041 ctctctccaa attagagcac cagaatcagc accacctgta tcctgaccag gacttaaaag
    47101 tacttttccg cactgcggta atcccttgag gcagtatcac tactagcgtc gggattgtat
    47161 cgccaacata gctgtttgaa tatgtaggca tcctgactta taattcactt gctagatgag
    47221 gactgtttaa tttctgcccg gaacaaccaa gtcttgggcg gaaaaaataa aaaaaccgac
    47281 tgtcatgtcg gttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggcgg cgcgttcatc gttattcatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtgggttgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgccgctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 gggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggaccaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattgatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc actcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agcgttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttaaaagttg gaaaattttt
    51121 ccaattaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctcaattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgatgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcact gggggtttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtagtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat tc
//
LOCUS       DAJTNM010000015        50290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTNM010000015 DAJTNM010000000
VERSION     DAJTNM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.015"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..335
                     /locus_tag="OO172_000994"
     CDS             <1..335
                     /locus_tag="OO172_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5511266.1"
                     /translation="LMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGM
                     LYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMI
                     CLLFIKDI"
     gene            446..1369
                     /locus_tag="OO172_000995"
     CDS             446..1369
                     /locus_tag="OO172_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5511267.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1373..2191)
                     /gene="nlpA"
                     /locus_tag="OO172_000996"
     CDS             complement(1373..2191)
                     /gene="nlpA"
                     /locus_tag="OO172_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR5511268.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2413..2706
                     /locus_tag="OO172_000997"
     CDS             2413..2706
                     /locus_tag="OO172_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511269.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2747..3937)
                     /gene="nepI"
                     /locus_tag="OO172_000998"
     CDS             complement(2747..3937)
                     /gene="nepI"
                     /locus_tag="OO172_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5511270.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4148..4600)
                     /locus_tag="OO172_000999"
     CDS             complement(4148..4600)
                     /locus_tag="OO172_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5511271.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4653..5987)
                     /gene="adeQ"
                     /locus_tag="OO172_001000"
                     /gene_synonym="yicO"
     CDS             complement(4653..5987)
                     /gene="adeQ"
                     /locus_tag="OO172_001000"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5511272.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6162..7928
                     /gene="adeD"
                     /locus_tag="OO172_001001"
     CDS             6162..7928
                     /gene="adeD"
                     /locus_tag="OO172_001001"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5511273.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPAVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(7974..9365)
                     /gene="uhpT"
                     /locus_tag="OO172_001002"
     CDS             complement(7974..9365)
                     /gene="uhpT"
                     /locus_tag="OO172_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5511274.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9503..10822)
                     /gene="uhpC"
                     /locus_tag="OO172_001003"
     CDS             complement(9503..10822)
                     /gene="uhpC"
                     /locus_tag="OO172_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5511275.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(10832..12334)
                     /gene="uhpB"
                     /locus_tag="OO172_001004"
     CDS             complement(10832..12334)
                     /gene="uhpB"
                     /locus_tag="OO172_001004"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5511276.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12334..12924)
                     /gene="uhpA"
                     /locus_tag="OO172_001005"
     CDS             complement(12334..12924)
                     /gene="uhpA"
                     /locus_tag="OO172_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5511277.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(12999..13289)
                     /gene="ilvN"
                     /locus_tag="OO172_001006"
     CDS             complement(12999..13289)
                     /gene="ilvN"
                     /locus_tag="OO172_001006"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5511278.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13293..14981)
                     /gene="ilvB"
                     /locus_tag="OO172_001007"
     CDS             complement(13293..14981)
                     /gene="ilvB"
                     /locus_tag="OO172_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5511279.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(15087..15185)
                     /gene="ivbL"
                     /locus_tag="OO172_001008"
     CDS             complement(15087..15185)
                     /gene="ivbL"
                     /locus_tag="OO172_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5511280.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15750..15839
                     /gene="tisB"
                     /locus_tag="OO172_001009"
     CDS             15750..15839
                     /gene="tisB"
                     /locus_tag="OO172_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5511281.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16119..17303
                     /gene="emrD"
                     /locus_tag="OO172_001010"
     CDS             16119..17303
                     /gene="emrD"
                     /locus_tag="OO172_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5511282.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17311..17808)
                     /locus_tag="OO172_001011"
     CDS             complement(17311..17808)
                     /locus_tag="OO172_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5511283.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17805..18167)
                     /locus_tag="OO172_001012"
     CDS             complement(17805..18167)
                     /locus_tag="OO172_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5511284.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18157..18504)
                     /locus_tag="OO172_001013"
     CDS             complement(18157..18504)
                     /locus_tag="OO172_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5511285.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLTWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18613..19026
                     /locus_tag="OO172_001014"
     CDS             18613..19026
                     /locus_tag="OO172_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511286.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19073..20456)
                     /locus_tag="OO172_001015"
                     /pseudo
     CDS             complement(19073..20456)
                     /locus_tag="OO172_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(20491..22206)
                     /locus_tag="OO172_001016"
                     /pseudo
     CDS             complement(20491..22206)
                     /locus_tag="OO172_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22373..23266
                     /locus_tag="OO172_001017"
     CDS             22373..23266
                     /locus_tag="OO172_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5511287.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23263..24077)
                     /locus_tag="OO172_001018"
                     /pseudo
     CDS             complement(23263..24077)
                     /locus_tag="OO172_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24077..25693)
                     /locus_tag="OO172_001019"
                     /pseudo
     CDS             complement(24077..25693)
                     /locus_tag="OO172_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            25988..26662
                     /locus_tag="OO172_001020"
     CDS             25988..26662
                     /locus_tag="OO172_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5511288.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26701..28362)
                     /locus_tag="OO172_001021"
     CDS             complement(26701..28362)
                     /locus_tag="OO172_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5511289.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28558..28986)
                     /gene="ibpB"
                     /locus_tag="OO172_001022"
     CDS             complement(28558..28986)
                     /gene="ibpB"
                     /locus_tag="OO172_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5511290.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(29098..29511)
                     /gene="ibpA"
                     /locus_tag="OO172_001023"
     CDS             complement(29098..29511)
                     /gene="ibpA"
                     /locus_tag="OO172_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5511291.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(29505..29577)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            29816..30148
                     /locus_tag="OO172_001024"
     CDS             29816..30148
                     /locus_tag="OO172_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5511292.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGSKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(30150..31364)
                     /locus_tag="OO172_001025"
     CDS             complement(30150..31364)
                     /locus_tag="OO172_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5511293.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            31465..32483
                     /locus_tag="OO172_001026"
                     /pseudo
     CDS             31465..32483
                     /locus_tag="OO172_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(32444..33781)
                     /locus_tag="OO172_001027"
     CDS             complement(32444..33781)
                     /locus_tag="OO172_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5511294.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33856..35004)
                     /gene="dgoD"
                     /locus_tag="OO172_001028"
                     /pseudo
     CDS             complement(33856..35004)
                     /gene="dgoD"
                     /locus_tag="OO172_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(35001..35618)
                     /gene="dgoA"
                     /locus_tag="OO172_001029"
     CDS             complement(35001..35618)
                     /gene="dgoA"
                     /locus_tag="OO172_001029"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5511295.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKVVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35602..36480)
                     /gene="dgoK"
                     /locus_tag="OO172_001030"
     CDS             complement(35602..36480)
                     /gene="dgoK"
                     /locus_tag="OO172_001030"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5511296.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLLR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(36477..37166)
                     /gene="dgoR"
                     /locus_tag="OO172_001031"
     CDS             complement(36477..37166)
                     /gene="dgoR"
                     /locus_tag="OO172_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5511297.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37444..38100
                     /locus_tag="OO172_001032"
     CDS             37444..38100
                     /locus_tag="OO172_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511298.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(38146..38958)
                     /gene="yidA"
                     /locus_tag="OO172_001033"
     CDS             complement(38146..38958)
                     /gene="yidA"
                     /locus_tag="OO172_001033"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5511299.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(39072..39470)
                     /locus_tag="OO172_001034"
     CDS             complement(39072..39470)
                     /locus_tag="OO172_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5511300.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(39710..42124)
                     /gene="gyrB"
                     /locus_tag="OO172_001035"
     CDS             complement(39710..42124)
                     /gene="gyrB"
                     /locus_tag="OO172_001035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5511301.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(42153..43226)
                     /gene="recF"
                     /locus_tag="OO172_001036"
     CDS             complement(42153..43226)
                     /gene="recF"
                     /locus_tag="OO172_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5511302.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPRFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43226..44326)
                     /gene="dnaN"
                     /locus_tag="OO172_001037"
     CDS             complement(43226..44326)
                     /gene="dnaN"
                     /locus_tag="OO172_001037"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5511303.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44331..45734)
                     /gene="dnaA"
                     /locus_tag="OO172_001038"
     CDS             complement(44331..45734)
                     /gene="dnaA"
                     /locus_tag="OO172_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5511304.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            46031..46111
                     /locus_tag="OO172_001039"
     CDS             46031..46111
                     /locus_tag="OO172_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511305.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46341..46481
                     /gene="rpmH"
                     /locus_tag="OO172_001040"
     CDS             46341..46481
                     /gene="rpmH"
                     /locus_tag="OO172_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5511306.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46498..46857
                     /gene="rnpA"
                     /locus_tag="OO172_001041"
     CDS             46498..46857
                     /gene="rnpA"
                     /locus_tag="OO172_001041"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5511307.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCLLARGS"
     gene            46821..47078
                     /gene="yidD"
                     /locus_tag="OO172_001042"
     CDS             46821..47078
                     /gene="yidD"
                     /locus_tag="OO172_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5511308.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            47081..48727
                     /gene="yidC"
                     /locus_tag="OO172_001043"
     CDS             47081..48727
                     /gene="yidC"
                     /locus_tag="OO172_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5511309.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48833..50197
                     /gene="mnmE"
                     /locus_tag="OO172_001044"
     CDS             48833..50197
                     /gene="mnmE"
                     /locus_tag="OO172_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5511310.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        12409 a        13526 c        12679 g        11676 t
ORIGIN      
        1 tattaatgct cattgctatc gtgagtggta tgtgttttta cgccagcgta ctcatggcga
       61 cgactccggc ggttgagctg gaattgcaaa ttctaaatgc catcttcctt ggtattctct
      121 gtggtatcgg catgctttat tttcaggact tgatgcctga aaaaataggc tctgcgacaa
      181 cgttatatgc aaatacttca cgcgtcggct ggattatcgc cggctctgtt gacggaatta
      241 tggttgaaat ctggagctac catgcgttgt tctggctggc gatagggatg ttgggtattg
      301 cgatgatttg cctgctgttt attaaagata tttagctcac cagtctgggc taagaaaaat
      361 cggcgtggcg caaacagagc catcctcgcc atttttgcta tcatgcctgc atacataaac
      421 gacaaaacag tatgcagagg gaaaaatggg ttccaccaga aaggggatgc tgaacgttct
      481 gattgccgcc gtgttgtggg gaagttcagg ggtctgtgcg caatacatca tggagcaaag
      541 ccagatgtcg tcgcagtttt tgactatgac gcgtttgata ttcgccggtt tgattctgct
      601 gacgctgtca tttgttcatg gcgataaaat cttttctatt attaacaatc acaaagatgc
      661 cattagtctg ctgatttttt ccgtggttgg cgcgctaacc gtacagctca cttttttgct
      721 aaccatcgaa aaatcgaacg cagccacggc aacggtgctg caattcctct caccgacgat
      781 tatcgtcgcc tggttctcac tggtacgtaa atcgcgcccg ggcattctgg ttttctgcgc
      841 tattttgaca tcgctggtcg ggactttttt attggtgaca cacggtaatc cgacgtcatt
      901 atcgatctct cctgccgcgt tattctgggg cattgcctcg gcatttgctg ctgcattcta
      961 taccacctat ccctcaacgc tgattgcccg ctatggcacg ttgcctgtcg tcggctggag
     1021 tatgctgatc ggcggtctga ttctattgcc tttttatgcc agacaaggga caaactttgt
     1081 cgttaacggc agtttgattt tggcgttttt ttatttgatc gtcattggta cgtccctgac
     1141 atttagtctg tacctgaaag gagcacaatt aattggcggt ccaaaagcca gcattttgag
     1201 ctgtgcagaa ccattaagta gcgcgctact ctctttgctg ttgctgggga tcacgttcac
     1261 attaccggac tggctgggaa cgctgctgat tctgtcatcg gtaattttga tttcaatgga
     1321 ttcccgtcgc cgcgccagaa aaataaatcg tccggcgcgg gatgagtgag aattaccagc
     1381 caggcaccgc gccaccgtta aagatggttt ctgccgcttt agcgacttcg gatgattgat
     1441 aagattgcag aaattccttc acgttttctg cattcttatt atcttcccgt gccaccaaaa
     1501 tattcacata cggcgaattt ttatcttcaa taaatacact gtcgtgcacc ggagaaagcc
     1561 cggtctgctg aatgtaagtg gtgctgataa tcgccacatc aactttggga tcatccagta
     1621 cgcgcgggag ctgcgccccc tcgagttcca taatctgcaa atgacgagga ttatcggtta
     1681 tatccagcgc ggtaggtaat aacccttttc cctctttcag agtaattagc ttctcttttt
     1741 gcaacagtaa aagcgcacga ccaaggttgg tcgggtcgtt ggggatcgcc actgtcgcgc
     1801 cttcttttat ttgcgccacg gttttgattt ttttggaata acccgccatg gggaagacaa
     1861 acgtatttcc caccgctacc agtttataac catgcgcctg attatcttgt tcaagaaagg
     1921 gtcgatgctg gaagacgttc gcatcaagtt cgccatgatt tgtcgcatcg ttcggtagca
     1981 acgaaccgct aaatcctacc agttgaacat cgaggccata tttctctttt gccacctttt
     2041 tagcgacttc cgcgacatct tgttctgcgc catttataac gccaacttta atgtgttttg
     2101 catcgctgct actctggtcg caacctgcca gcagaattcc ggccagcaat aatgcggccc
     2161 ctgtccgtag atgatgtgtt gtcagtttca tttttttatc cttttattaa tgattacctg
     2221 actatagcgg taaggtcgct gcggtttaaa aaacgaaacg ctatcgataa gaataaaaag
     2281 gaataaaagt ggaatataaa gtctggcact tcttactgac cacgcaagcg cgtttcgtac
     2341 agcatgatga gagcgatgaa agcaaactac acttatgctt cattcgatac acatttgtaa
     2401 aaggttgaat ccatgaaacc aacgacacta ctgcttattt tcactttttt cgctatgcca
     2461 ggcatcgtat acgccgagtc gccttttagc tcactgcagt ccgcaaaaga gaaaaccacg
     2521 gtattacaag atttacgtaa aatttgcacg ccacaggcgt cattatcaga tgaagcgtgg
     2581 gaaaagttaa tgttgtcaga tgaaaacaat aaacaacata tacgcgaggc catcgtggcg
     2641 atggaacgta ataatcagag caattattgg gaagcgttgg gtaaggtaga atgcccggat
     2701 atgtaagaac agcaacaact actgaccaac gcttctttac ctgatatcag gatttcttca
     2761 ttttcacctt tgcagtaacc aacaatgcag tcagcaacat caatgtgccg gacaacatca
     2821 acggcgaagt cagaccaata ttatccagcg cataaccgcc gattgccgcg ccacaggtat
     2881 tagcaagctg aataaccgcc acctgaatag acccggcttt ttctgcctga tcggccagcg
     2941 agcgggtgat ccacgttgac cagccgacgg gaaccaatgc aaaagttagc ccccagataa
     3001 tcgccacgcc ggtagcaacg attttatcgc ttccccacaa cgtcagtacc aacgcactca
     3061 cagccagtat taacggcgcg cctgctaagg ccagttttac cgaacgttta agaatgaacg
     3121 acgaaagcga cgtaccaata aagctggcga taccaaaact caacagcacc agcgttaagc
     3181 catccacgcc gaatcccgcc aggttcatat acaccgggcg aatatacgtg aagaaagcaa
     3241 actgcccggc gaaagacatg aagatggcga tcatccctgc catcacaccc ggacgttgta
     3301 ataagcggaa agtattttgt ttctgatgcg agggttcgcc tggcagtgaa ggcaatgatt
     3361 tgataatcca gaaaatacac agcacgccca tcaccgccgc cgcattaaag acattgcgcc
     3421 aaccgataag ctcgcctaaa aaactgccca acggcgcggc aatcaccagc gcaatagaaa
     3481 ccgcgccgaa gatcaccgac agcgccttcg gcaccgtacg cggcggtacc agacgcatgg
     3541 tcagcgacgc cgacatcgcc cagaacccgc ccagcgccag ccccagacag gcacgaccga
     3601 ttaaaagcaa actgaatgag ttagcaaagg aaaccagcaa gcaggagagc gtcagcaaaa
     3661 cggcaaacaa aataacaacg tagcggcggt cagtggcctg aattgtctgg gtaataaaca
     3721 aactggcaaa cattgccaca aaggcggtca cggtcaccga ttgcccggca accccttccg
     3781 aaatgcctaa atcctgggcc attggcgtca acaaactgac gggcaaaaac tcaacgataa
     3841 tcagacaggc gacacaaaac gccaccgaga aaacggctga ccagttcggt cgggtgatgg
     3901 catccgcgcc gcggttttcg gcaataaatt cactcatggt gttacccgtg gcatgtttca
     3961 ggggaaaagc cgaccacttt aacattgaaa tgtgtgacgc atttaacgtt tttgcaactt
     4021 ttacggtgtt gcttgagctg gagttaagat cgaaacggaa cagggcaaag attaccggat
     4081 gcggcgtgaa cgtcttatcc acccgacagg tcaggcgcgg tctgtaggct gataagacga
     4141 aacggtttca atgcaaccag tgaacgccct cggcaggctg aaagaacgcg ttatagcgca
     4201 tctgaaaagc attaatatcc tgcatttcag gttcggtttc acgcagaaaa cccagcgcca
     4261 gcctgacctg agcatcggta atcggcgcgg aaaggcgggc tttttgtaaa atccggtgcc
     4321 attgcagcag gttctgttcg ctatcatcga caatgacaat ttgccagata agcaacacct
     4381 gcgcggcgtt ttgcaggtgc gactcatccg ggcgatgcaa ataacgtaaa aattcgtcac
     4441 cggctgactt tcccatttga tcgaccatcg actccacggg tacgacatcg atattcagcc
     4501 gatcgctgag acggcgaatc gcttttctgg ccccggatgt cagcacccga aaaccaacca
     4561 caaacactac cgccagcgtg gcgagcatta tccagatcat gagttctcct gcgtaatcag
     4621 aactcatgat atctggaaac cctcgccagt tactaatcca ccagaataat cttcaatgcg
     4681 aacagagctg cgaccaccac cacacacagg ttcagatcgc gccagcgccc ggtgcacact
     4741 ttcatgatgc agtacgacat aaagccgagt gcaatccctt cggtgatcga gaaagtaaag
     4801 ggcatcatca ccgtggtaat aaacgcaggc accgattcgg taaaatcatc ccagttaacg
     4861 cgcgccaggc tcgaagtcat cagcacgcca acaaagatta acgctccagc ggttgcgtaa
     4921 ggaggaacca tcgccaccag cggtgagaag aacataacta acaggaacat aacgccgacc
     4981 acgacagccg ttagccccgt gcgaccaccg actgccacac cagaagtact ttcaatatag
     5041 gcagtaacag acgaggtacc gataaatgcg cctgccaccg aactgacgct atcaacatac
     5101 agcgccttat tcatattggg gaatttaccg ttgctatcta ttaagcccgc tttatcagtt
     5161 acaccaatta atgttcctga tgaatcaaat aggttgatca gcataaagga gaaaatgata
     5221 ccggcgagtt caagtgttaa cgcgccgctc aaatctactt caccaatgac gccgctaata
     5281 tcaggcggaa tggaatagac gccgctaaaa tgaacatcac cgaaaaataa tccacagcaa
     5341 gacgtcacca caatagaaac cagcaccgcg gcatgaaaat gacgtgatga caacacggtt
     5401 ataataaaaa accctaaaat acctaacaac acgccgtgag aacttaaatc gccaatcatc
     5461 accagcgtgt ctttattggc gacaataaca ccagtgtttt ttaatcccat taaggcaata
     5521 aataatccga ttccactggt gataccaata cgtaaactta atggaatgtt ggagatcatc
     5581 cagtaccgga tacgaaacag cgtgagcaaa aatagcccaa ttgcgcccca gaatatcgcg
     5641 cccatcccgg tctgccagga gatccccatc gcccccacga ccacgaacgc aaagaaggcg
     5701 ttcagcccca ttgccggagc cagcaccacg ggtaagttag caaatatccc catcgcaata
     5761 ctgccgatac cggcaatcaa acaggtggta acaaacacca ctttcgggtc catttgtgcc
     5821 gcgccgagga tttgcgggtt cacaaaaaca atgtacacca tggttaaaaa agtggtcatc
     5881 cccgcaatca attctgtgcg gacggtggtc ccatgctgag gtagtttaaa taatcgcgaa
     5941 agcgtccctg attcattact cacgtaatcg gtattgtcat tattcatttt tttgtccatt
     6001 ggaggagatt taatcccttg cttttgtaaa acaaatgaca acaacggttc agtgataatt
     6061 atcacatttc aattgcacat taatagatat tctttaataa tctcgcgacg tttctttatg
     6121 ataaataata atcaaattga taaaatcaaa atgagaaaaa tatgaataat tctattaacc
     6181 ataaatttca tcacattagc cgggctgaat accaggaatt gttagccgtt tcccgtggcg
     6241 acgctgttgc cgattatatt attgataatg tctctattct cgacctgatc aatgccggag
     6301 aaatttccgg cccaattgtg attaaaggac gttacattgc cggtgttggc gcagaatacg
     6361 ctgatactcc ggctttgcag cggattgatg ctcgcggcgc aacggcggtg ccagggttta
     6421 ttgatgctca cctgcatatt gaatccagca tgatgacgcc ggtcactttt gaaaccgcta
     6481 ccctgccgcg cggcctgacg accgttattt gcgaccctca tgaaatcgtc aacgtgatgg
     6541 gagaagccgg attcgcctgg tttgcccgct gtgccgaaca ggcaaggcaa aaccagtact
     6601 tacaggtcag ctcttgcgtg cccgctctgg aaggctgcga tgttaacggt gccagtttta
     6661 cccttgaaca gatgctcgcc tggcgggacc atccgcaggt taccggcctt gcagaaatga
     6721 tggactaccc tgccgtaatt aacgggcaga atgcgctgct cgataaactc gatgcatttc
     6781 gctacctgac gctggacggt cactgtccgg gtttgggtgg caaagaactt aacgcctata
     6841 ttgctgcggg tattgaaaac tgccacgaaa gttatcagct ggaagaagga cgccggaaat
     6901 tacaactcgg catgtcgttg atgatccgcg aagggtccgc tgcccgcaat ctcaacgcac
     6961 tggcaccgtt gatcaacgaa tttaacagcc cgcaatgcat gctctgtacc gatgaccgta
     7021 acccgtggga gatcgcccat gaagggcata tcgatgcctt aattcgccgc ctgatcgaac
     7081 aacacaatgt gccgctgcat gtggcatatc gcgtcgccag ctggtcgacg gcgcgccact
     7141 ttggtctgaa tcacctcggc ttactggcac ccggcaagca ggccgatatc gtcctgttga
     7201 gcgatgcgcg taaggtcacg gtgcagcagg tactggtgaa aggcgaaccg attgatgcgc
     7261 aaaccttaca ggcggaagag tcggcgagac tggcgctgtc tgccccgcca tatggcaata
     7321 ccattgcccg ccagccagtt tccgccagcg actttgccct gcaattcacg cccggaaaac
     7381 gctatcgggt cattgacgtc atccataacg aattgattac gcactcccgc tccagcgtct
     7441 acagcgaaaa tggttttgat cgcgatgatg tgtgctttat tgccgtactt gagcgttacg
     7501 ggcaacggct ggctccggcc tgtggtttgc tcggcggctt tggcctgaat gaaggcgcgc
     7561 tggctgcgac agtcagccat gacagccata atattgtggt gatcggtcgc agtgccgaag
     7621 agatggcgct ggcggtcaat caggtgattc aggatggcgg cgggctgtgc gtggtacgta
     7681 acggccaagt acaaagtcat ctgccgttac ccattgccgg gctgatgagc accgacacgg
     7741 cgcagtcgct ggcggaacaa attgacgcct tgaaagccgc cgcccgtgaa tgcggtccgt
     7801 tacccgatga gccgtttatt cagatggcgt ttctttctct gccagtgatc cccgcgctaa
     7861 aactaaccag tcaggggcta tttgatggcg agaagtttgc cttcactacg ctggaagtca
     7921 cggaataata aaaaaagccc ggcgtcatgc cgggcaaaag tcaccagtta cgtttatgcc
     7981 actgtcaact gctgaatttt tttctcgcgg cggattttgc gttcttccat taccgccact
     8041 atcgccatca ggcagataca accaatcgcg gcgatatcca gcgcggcgaa ggtgcctgcc
     8101 cagccggtaa ggccgaatac cggcgtccca tcggcaatca ttcccagacc taacttggca
     8161 aagctgtcac caatcaggta agcaaaggtg cctttaatac catcggcagc gccaatcgct
     8221 tttttaggta caaagccaac agcagccaca ccaatcaaca attgcgggcc aaagaccagg
     8281 aaacccaacg caaagagaga agccagatag atatattcgt tactggcatg ttgatacaca
     8341 ccgagcgtgg cgataatcag cgccagcgcg atgcaggcca ccaggccacg gcgaccgttc
     8401 gccaggtcag agagccagcc ccacagcagc gtaccgacca gcgcaccagc ttcaaacagc
     8461 gtaaagccct gaatcgccac cgctttagag agtttcagtt cctggaacgc gtatacggtt
     8521 gaccactggt cgataccaat acgtaccaca tagaggaaaa tgttggcgaa gcacagcagc
     8581 cagatcactt tgtttttcag cacatactca acaaagatct gccacttggt catatcggta
     8641 gattctgtct ctttgtcctc ttcgctgacc tcctcgccga acagttcttc agctttgccg
     8701 aggccataag attccgggga gtcgctaccg taacgcaggc cgataaaacc gacaatcagc
     8761 gcgataatcg acgggaagat aaacatgccg atgacatggc catcgaacag gtaatttgcc
     8821 ccgaacagcg ccacacctgc tgcgcctgca ccgccaaggt tgtgagaaat attccagaaa
     8881 ccgaggaatg tcccgcgttt acgacgcggc gtccatttgg tgatggtgga gtaactgcac
     8941 gaaccgccgg tactctggaa aaagccgctt aaggcgtaga aggcaatcat caggaacagg
     9001 ctaaccgagc cgctgcccat actggcactg aagcccagca tacaaatggc agagaggata
     9061 agcatgaacg gcaggaattg tttggtgttt ttgccgtcgg cgtagtatga aaccaacgtt
     9121 ttacccacgc cataagtgat ggagaaaccc aggccgatca tccccagctg cgtcatgctc
     9181 aacccgtagg tcgaaatcat atcgttctgc gcgatgttaa agttcttgcg aatcaggtac
     9241 atcgtcaggt agccgataaa gaccaccagg taggattgca tgaacggttt gaaccacatt
     9301 ttgcgccgca cttcgagcgg aaggtccagg gtcggcttgc gaacctggtt taggaaagcc
     9361 agcatgggtt actcctgaaa tgaatacctg cgtgaggcat gcattgtaaa aatcagcagc
     9421 aaatcaggcc tgagatagcg tccaagaaaa acctggaaaa tttcttagtt ttgccggata
     9481 tgaagtgaaa aggtgagatg catcacgctt cacgcggtgt ctgggcgttc aaaaagggca
     9541 gtaacagcag tgcggaaatc ccggcggcga tagcgataac cacaaaaaat ccgctccagt
     9601 gccaggtatc gagcactttc gccagcggcc aaccagcaag cgacgccccc agataagcaa
     9661 acaagccgac aaaccccgtc gccgcccctg ccgcctcttt gtgggaacac tctgccgccg
     9721 ccataccaat taacatctgc gggccaaaga caaaaaaacc aatggtgaag aagcaggttg
     9781 cctgcatcac gtagctggca aatggcatca gccacagcga gccgactgaa agcaaaattc
     9841 cggcggcgaa aatcaaattc atcggcccac ggttgccgtt aaacaatttg tccgagcccc
     9901 aaccggctac cagcgcaccg ataaatccgc ccagttcaaa catcgtcact gccgtattcg
     9961 ccgtgaccag atcgacgccc agcgtctcgg acatatacaa attgccccag tcgttgatcg
    10021 ccgcccggac cacatagacc agcacatagc aaaacgaaag cagccagata tacggattca
    10081 gcaacacata tttggtgagg atctctttac gcgtcaaccc tgccccttct tgttgttgag
    10141 caatttccag cgcgtcgtgc cgccattcac cgaccgccgg taaacctaac gcctgcgggc
    10201 gatcgcgtag ccgccagcag agaaaaatcc ccacgactat cgccatacaa ccagcaatca
    10261 tcatcccggc acgccagccg taatgcagcg cgctcgctgc catcacaatg ggaatgagtg
    10321 cgccgccgac gttatgcgcc gtgttccata atgcccacca accgccgcgc tcggtacgtg
    10381 aataccaggc cgttaacaga cgcgcacaca ccggtgaacc ccagccctgg aaaaaggcgt
    10441 tcagcaccca gagcacggca aacgcccata gcgacgtcga gaagccaaac agaatgttca
    10501 tgatgcccgt ggcgataagc cctatcccca taaaataacg ggcatttgag cgatcgctga
    10561 caatgccgga gacaaacttc gacacgccgt aagtaatgta aaacagggtc gctaacaggc
    10621 cgatatcgct acggctgagc acgccgttag caaggatttc tggtaccgcg gcgttaaaac
    10681 ttttccgcgt gaagtaaaac agcgcgtaac ccagccagat ggtcagcaga atatgccgac
    10741 gccagtagcg ataacgggca tcaatttcat gtttatcagt cattaatggc gcatcggcag
    10801 gcgctttcag aaacggcaac atcatcgaac cttagacata acgttgaggt agagaaacgc
    10861 tgacacgcgt gccgtgcaga caggaaatgt gtaatgtgcc gcccagcgcc gttacgcgct
    10921 cgcgcattcc ggtgaggcca aaaccttgtt gcccggaacc cggcggcaaa ccgctgccat
    10981 cgtcttcaat caccagcatc aaccgttcat cctgctgcca gccttgcagc gtaaccgcgc
    11041 tggcatcggc atgtttcaca atgttgttca gcccttcctg gcagacacga aacagcgtca
    11101 cgcgctggtt ttcgcttaat gctgattcat cgatacgcca ttcgagatgg ctgacaatgc
    11161 cgcgcccttc cagctccatt tcccgcatca gtgagcggat ggcctgctcc agggtgagat
    11221 catccagctg gcgcggacgt aaccgtccta acaaacggcg caccgcgtcg taaacgccca
    11281 gcgacagttg ttcgatgagc tgcccgctct gcttcacact ggcgttatct gccgccagcc
    11341 gctgaacaat gcccgcctga gtacgaatag cagtgatggt ctgaccgata tcgtcgtgca
    11401 gctcacgcgc cacgtcgcgg cgcacgctct cttcggtttc taacaaacgt tcagccagat
    11461 gctgattgcg cgccagttcc ttttgtagcg actggttaag ttcacgcaac cgctggatgc
    11521 ctgcgcccag taacaaccct gtcagacttt gcaccagcaa cgagagtaat aaatccacca
    11581 gatgatcgcg ccaggtttga ctggcgatca gcgcgatggc gttcatcaac gtcgcaatca
    11641 gcgctccttg ccagccatag tgccaggcca gcgcgataat cggcagcgcc agacagaatg
    11701 gcgtaaagcg cgacagttcg tccggcaatc ccaactggag ccagagactg ataacaaaca
    11761 gcagcaagta ccagaccaga tgccgcccgc gccagttgat gggctgagaa accagtgacg
    11821 ggccgagcgg cagccaggtg ttattggcga gatagtgcca gaacaccaga catatcgggg
    11881 ccagcgtcag accgccagtt aaagtcagca acagcgcatt ccacgactct ttgccgtgcc
    11941 aaagccaggg cagcgactgc aacaacgccg ccgccgttaa cgccgcaccc tgtaacagca
    12001 aggtgcgcca gtcacgctga tggcgatagc gcgagatcag cgctacgggc agtaacgtca
    12061 gtaaactacc gatcatcaat aacggaaaat gggttaaacc gaccgcctgc gttagccagt
    12121 aaatcagcag ccactccgca cccagcaata cgggccagta tccgcgcggg cattgcagca
    12181 ttagccccag acgcagacca aacggaaata acagcaccgc catatcaggg cgctcaacca
    12241 gatgcaggct gatactccat aggcaaaacc atgcggcaga gaagataaaa aagcaggcaa
    12301 taacggtaat taagcgggag aacaacgtct tcatcaccag ccatcaaaca tgcggcgcgc
    12361 cagctctacg tcgttactga cgcccagttt ttccatcaga ttggcgcgat ggacgtgtac
    12421 cgttttcggt gacaagccca gttcggcggc aatctctttc accgccattc cttgcgccag
    12481 tttttccgcc acctggcgtt cacgtttggt cagcgggtcc tgacgaccgg atgccagttt
    12541 aatggcaata tccggcgtca gataacagcc gcccgtggca accgtatgca ccgcagcaat
    12601 gagttcatcc gggctacagc gtttggaaag aaagccgcgt gcccccgcgt taagcgcctg
    12661 ctcaaccagc gcaggactgt cgtgaacgga gagcataatc gtcgccatac ctttcggcag
    12721 ctggcttagc agctccagac cggagatatc gggcatggag atatcgcaaa tacacacctg
    12781 cacaccgcgc cccggcagcc ccgccagcgc ctcgcgcccc gaaccaaact cggcaactac
    12841 ctgcaaatca ggttccagcc ccagcagctg cgcaaagccg gagcggacga tgaggtgatc
    12901 gtctataagg gcaacggtga tcatggtctt gtcctggcgg gtaaaaaaaa acgcgcttac
    12961 cttaacgata agcgcgatgt tgttcaagcc ttgagcggtt actgaaaaaa caccgcgatc
    13021 ttgttaaaca tcgtcggatc ggactgatta cgctgcactt tcacgacatc ttccagctta
    13081 tcgatttggc ttatcatctg ctccagacgc tggtcgtcat tgaccagtag ccagatatgg
    13141 cttttgtcgc tgtcctgaat cggcagacaa agaatgcctt caacgttaaa agcgcggcgg
    13201 gcaaaaaggc cacaaacgtg ggtcattacg cccggatggt tgcgaacggt gagctccaga
    13261 attacgttgt tatgagttgt gttttgcatg gcttattccc ccaccatttc agtattcgcc
    13321 gcacctggcg gcaccatcgg gtaaactttt tcttcggcat caatgcgcac atggatcagc
    13381 gccgggccag ggcgattgat tatttcctgc aatgcagcct gcggatcggc ttcgttattc
    13441 aaatcacagg tttcgaggcc gaatccggcg gcaatctgca taaagttgat tttgcccgga
    13501 taggtggcgg caaaaacgcc ttgctcgtag aacagactct gttgctgatg caccagcccc
    13561 agcgcttcgt tgttcatcag aatgattttg acatccagct gattttcact ggcggtcgcc
    13621 atctcctgaa tattcatcat caggctgccg tcgccggaga aacacaacac tttgcgatcc
    13681 gggttcgcca gcgccgcgcc aatcgccgca ggcaggccaa aacccatcgt gcccagccca
    13741 ccggaggtca gccactggcg tgggcgattg agcggataag cttgcgcggt ccacatctga
    13801 tgctgaccca catcggtggt gataatcgca ttgtcatcga cacaggcggc aacggcgttg
    13861 atcaggccgt aatggcttaa tggatcgcac gctttcggga ttggacacgg gaattcgcgc
    13921 tgcaaatccg ctaccaactg gtgccactct gcacgcagtt gcgcttccac ctgcgggatc
    13981 aattgcgcca gcacgtcatc aacatccgcc tgaatcgcca catgcggctg cttgatttta
    14041 cccagctctg cgcgatcgat atcgacatga ataattttgg cattcggaca gaactgctcg
    14101 gttttgccaa tcgcccggtc atcaaaacgc gcaccgagca caatcaacaa atccgcctct
    14161 tgcaaaatat agttggtact gcgcacgccg tgcatcccca gcatacccag cgacaacgga
    14221 tgcgcttttg gcagcatgcc cagcgccatt aaagtcatgg tggtaggcag ttgcgctttc
    14281 tccgccagtt cacgcacccg cgcaggcgca ttgatcacac caccacccag ataaagcacc
    14341 gggcgtttgg cagcgttaat cattgtagct gcgtcacgaa tgctttcttc gctaaaggag
    14401 ggtgcagcgg ctttttctgc caccgcgggc tgagcttcaa tctcaaaaac cgccgtttgt
    14461 acatccttag gaatgtctat ccacaccggg ctagggcggc ctgattgcgc aatgcggaag
    14521 gcatcgctca tgacctgtgg gagctcttcg atatgtctga ccagatagtt gtgtttggtg
    14581 atggggatag agatgccgta ggtgtccact tcctggaagg cgtcggtgcc gatcatcgag
    14641 gcgggaacct gaccagtgat gcaaatcagc gggatggagt ccagccgcgc atcagcaatg
    14701 gcggtcacca ggttagtcgc acccggcccg ctacaggcca tacagaccgc cggtttaccg
    14761 tcggtgcgcg ccattccctg agcgataaac cccgcgccct gttcatggcg agccagaata
    14821 tggcggattt gcgtgctttg gcttaaggcg tcgtaaacag gcaggataga accgcccgga
    14881 atgcccgtca caatcttaat gccctgctgt tccaggaaat gaacgataaa ttctgcgccg
    14941 gtaaagcgct tacgcgtcga tgttgtgccc gaacttgcca tgctccagtc cttttcttct
    15001 gggccgactt tccgggaggt gcttaaacga aaaaccccgc ccggtttgcg ccggcggggt
    15061 tttggaatcg tgtgttgttc cagtccctac ggcgcattgc cgacgaccac caccacacgc
    15121 acgacgacca ctgcggcgga tggcgcagtt ggtagtagtt ttgcgttgaa catggaagta
    15181 gtcattgggg accttgttgg ttttgtgttt aacaatattt atacaagcac agctttacag
    15241 gggagacaat ggaaaatttt tcagcaaggg aaaattgagg ggttgatcac gttttgtact
    15301 gaattgcaga taacaaaaaa ccccgccgga gcgaggtttc gtcagccgcc tgcggctggt
    15361 aaccgcaaag cacactgtat tatgtcaaca ctgaaagtat acgtgttccg cgcagaacgc
    15421 gcaatttcgg cacgaatttt gacgtattta gtgcatagtt gagtatcgtt cacagtttgc
    15481 gttttgtcca aatattactg tttatttata cagtaaactt ctataatatc actgttcgca
    15541 atgtgttatg cgggggccgc atcgttaccc ggcgcactaa gtcctggctg aaacgggtgg
    15601 tgccgtcagc gccttaaccc cgcgtgagca cactgtgtta tgtcaacaag cacaacgttt
    15661 ctccttgaga taccgcgtgc acaacagctg gcaacaggca gcggaaaggt acgtcagctg
    15721 gcagtgctcc tgaaccacag gagacgcgta tgagcctggt ggatatcgcc attcttatcc
    15781 tcaaactcat tgttgcagca ctgcaactgc ttgatgctgt tctgaaatac ctgaagtaat
    15841 tcagattcaa gtcgcaccaa aggggagcgg gtaaccgctc cccttttata tttagcgtgc
    15901 gggttggagt cggatgcgat gctgacgcat cttatccgcc ctaccatccc tcccggcaac
    15961 atttattgcc gcttttgttt acatattctg ccgctaaaca attccccatt cctggcgtat
    16021 atctggctaa cattcatcaa tgtgatagat tcctctcccg catttatggg aatgcgtagt
    16081 gacttattct aattattttt ataaaagcat ccgtgataat gaaaaggcaa agaaacgtca
    16141 atttgttatt gatgttggta ttactcgtgg ccgtcggtca gatggcgcaa accatttata
    16201 ttccagctat tgccgatatg gcgcgcgatc tcaacgtccg tgaaggggcg gtgcagagcg
    16261 taatgggcgc ttatctgctg acttacggtg tctcacagct gttttatggc ccgatatccg
    16321 accgtgtcgg tcgccgacca gtgatcctcg tcggaatgtc catttttatg ctggcaacgc
    16381 tggtcgcggt cacgacctcc agtttgacgg tattgattgc cgccagcgcg atgcagggga
    16441 tgggcaccgg cgttggcggc gtaatggcgc gtactttgcc gcgtgattta tataaacgga
    16501 cacagttgcg ccatgctaac agcctgttaa acatggggat tctcgtcagt ccgttgctcg
    16561 caccgctaat cggcggtctg ctggatacga tgtggaactg gcgcgcctgt tatctctttt
    16621 tgttggttct ttgtgctggt gtgaccttca gtatggcccg ctggatgccg gaaacgcgtc
    16681 cggtcgatgc accgcgcacg cgcctgctta ccagttataa aacgcttttc ggtaacagcg
    16741 gttttaactg ttatttgctg atgctgattg gcggtctggc cgggattgct gcctttgaag
    16801 cctgctccgg cgtgctgatg ggcgcggtgt tagggctgag cagtatgacg gtcagtattt
    16861 tgtttattct gccgattccg gcggcatttt ttggggcatg gtttgccggg cgtcctaata
    16921 aacgcttctc aacgttgatg tggcagtcgg ttatctgctg cctgctggct ggcttgctga
    16981 tgtggatccc cgactggttt ggcgtgatga atgtctggac gctgctcgtt cccgccgcgc
    17041 tgttcttttt cggtgccggg atgctgtttc cgctggcgac cagcggcgcg atggagccgt
    17101 tccccttcct ggcgggcacg gctggtgcgc tggtcggcgg tctgcaaaac attggttccg
    17161 gcgtgctggc gtcgctctct gcgatgttgc cgcaaaccgg tcagggcagc ctggggttgt
    17221 tgatgacctt aatgggattg ttgatcgtgc tgtgctggct gccgctggcg acgcggatgt
    17281 cgcatcaggg gcagcccgtt taagcgcacg tcaccgcagc atcgtcatca gctccatggg
    17341 agaacgatgc tgctttatca gatcacgcat cacccgcata tgcggtgcgg agtaagaata
    17401 aaacgcctga tagcccgcac aaagcacgct gtctggcgtc tcctgccgag cgtgcgggca
    17461 caggcgatgc cagtcgcacg cctggcagtc acggcgcggt tgcgggtttt cgctgccaca
    17521 ccatttttgt aaggtggcgt caaaaagctc aatcgagata cgactgatat ctgcccgcac
    17581 ccaacactca aacagggcaa ttaaaaattt cccccacagc tctgctgtca ccgaagcggc
    17641 cgtcagatcg ccccggtcat caaattcaag atggggaatg agctgtacgg agcggatgcc
    17701 ccgctgtttg aaattgtgat aaaaatcggc aggttgcaga gcatccttgt actctacaac
    17761 cagcttatgc ctgtcctctt cagcatcaat gacctgactt cctgtcatgc gactctctca
    17821 ataaatacga taagttgatg tatatgcgtt acagcaaaca gtattgcgag agataacacc
    17881 gcgagggaga tcaaaaattt gtcacggact acgtgaaatt gggaaaaatc gctattcgtg
    17941 acatccatta aattgcgatt acgggtgtag tgccagagga tcagcgccac gatggcgagg
    18001 atgccaatcg aaatccagaa taacatgccc gcctggtgcc agttgtgttt gatagccaac
    18061 gccatcaacg cgccgtagcc cagcatggta cgaaaccacg ccagtgatgt gcgctccggc
    18121 tgcagccctg ggtcggcaat gcgtctggct ttacggctat ccggcataca acaccagtcc
    18181 cataacgatc accgccacaa ccatcaatat taagctgatg attaataagc tgttggtgta
    18241 gggcaggtcc tctttcagac gcatcgcttt ttcattgcgt aaccagcgca ggtagccata
    18301 cattgctaac ccgccggaaa acagacacaa caacaacgcc agcagttcac gaatgacggg
    18361 cgtggcgaaa tctggcgcaa gttgatcaag cccaaccccc gccgccagaa atcctagtgc
    18421 ggtacggatc cacgtcagaa aagtacgctc attagccagc gagaagcggt aatcgggcgc
    18481 ttctccaagg cgggaaattt tcattgttgc tcctttgcaa tttcactaac gctctatctt
    18541 tagcggtttt ttttaagaat ttcgtccgcc cgtttcctga tatgcttttg ccatccgcta
    18601 caaggaggtc agatgggtat catagcgcaa aataaaatca gttcattggg catgctgttc
    18661 ggtgccatcg ctttaatgat ggggattatc cacttttcat tcggcccttt ttccgcacca
    18721 ccgccaacgc ttgaaagcat cgtggcggac aaaacggctg aaattaaaag aggtcttctc
    18781 gccggaataa aaggcgaaaa aattaccact gtggagaaaa aagaggacat ggatatcgat
    18841 aaaatcctcg atcaaagcgg tatcgcactg gccattgctg cgctactttg cgcatttatt
    18901 ggcggtatgc gtaaggaaaa tcgctggggc attcgcggcg cgctggtttt tggtatcggc
    18961 atcgtgtgca gcattttgct gatattcctg attttttcct tcctgaccgg cggttcgctg
    19021 gtttaagcag cctgaataat gcccgccggg tgatcatcag cgggcattat cgtcagaatt
    19081 tctgtttttt ctcctccacc ttcactcctt gcgtcggtag gccggtgtca tagtcgcgta
    19141 ccactggcga atagccatct tgtgggcgtg ggcgaaacgc ccccatccag cgcggtcgtg
    19201 catctttacg ccacggacgc agactccatt ggtaactgcg gaacggatcg cgaattttgt
    19261 ccatgtaatc caataaggcg tcatgcattt tgctgcgaac gtctgcaaaa cggatatcat
    19321 cgatcaggtt atgcatttca tttgggtcat tacggcgatc gtaaagttca tcactggtga
    19381 agaggttgag caccagttta aagtcatccg tcacccagca acgcaccgga ataaaaccgc
    19441 caaagctgtc atgctcaatc tcgtagcggt taaattccac catcacgccg cgtggctctt
    19501 tcacggcaag gatattttcc cccggcagaa tctctggttt ttcaatatct gccagcgcca
    19561 tcattgtcgg cagtaaatca atatgactga ctggcgtatc gacctgtcgc cgctccttta
    19621 ctgatcagct tatgtgcgcc catcatttca ccgtgatcgg aggtataaat gacccacgta
    19681 ttttcacgtt gctctggcgt taaggcattg atgacacgtc cgatttggtc atcaacaaag
    19741 tcattgcagg caaaatagag cggatggtga taaagcccgt catcaccgac tggcgatggc
    19801 atcgcctgcg cccataagcg gtgatgttcc ggtttgttcg ccaggtcatc ctgtgatttc
    19861 tcgccaagtt cgtagtaaaa atcagcgtat ttctctaaat actccaccgg acaggtgaac
    19921 gggtgattcg gctcatcata cgaaaccacc atcaggaagg gttcctcggc gcgcacgggc
    19981 tgttgcagaa aatctaccgc tcgattgctg atgcggtgcg cccaggtgaa ggtttcgtca
    20041 atatggttcg cctgtaaatc ttcgacgctg tttaggccat tgcgccacag gctgatctct
    20101 ttttccgtca gttcgctaag atagttcgcc ccatcaaacc agtaatcagc atcccactcc
    20161 ggcggacact cgccagtgcc gaaatagtca tgaccgtcga gatgccattt gccgatgtaa
    20221 caggtgtgat agccggcatc cttaaagtag cgccccatag tggagatgtt tttgcctggc
    20281 gcgacgttgt tggtccacgg gccggactgg ttagcgtaga taccggtgaa tagtccggcg
    20341 cgtgcaggcg tacaaaccgg tgaacaagtg taggcggaat taaagcgaat accttccgcc
    20401 gccagactat caatattttg cgtattcagc ggtttaccgc tatagcaacc gaccatattg
    20461 gtggcctggg tatcggtcaa ttggggcgtt tcataggcta tccttcgcgt cgggagtaaa
    20521 ggttacggct gggtcgtgac gatgccgcca gctgtcaaaa atcaggtaga caatcactac
    20581 ggcagcgatg aaataactga tcatcgctaa ccagcgcgta ccgtagccgc cgaattcagc
    20641 cagcccgaca tagacgccaa tcatcgcgaa caggatacca attgacgcga tcttgacgtt
    20701 tttccacggt ttcatgtcca ccgcaaacgc atctttgaag gtgaacggcg tggcgcgcgg
    20761 tttgataaaa ccgatcacca gcatcacgac cacgttgatg cagaacgtac aggccagcac
    20821 atagaggaaa tggaagtcga acttcaccag atagttgatg gtgatgtagc tgattatgcc
    20881 aatccccatc gccacttttg ccgccagcgc cgggatgcgc gggaagaaaa agcccataat
    20941 gatgatggta accagcggca cgttgtaaat gccgttgagc tgtttcatcc agctatacag
    21001 cccctgcggc gcgttggcga tccacggcgc taccaaaacc gaaacgatag cgataaagaa
    21061 accaaatttg cgcccgacgg tgaccagctg ctgcggctcg gcattctggt taatgatgcg
    21121 acggtaaata cccatactga ataatgtact ggcgctattc agaaagccgt tgaaggtact
    21181 gatcaccgca ccaaataaca ccgcgccgaa gaaacccacc agtggcactg gcagaacgtt
    21241 attgaccagc gtcgggtagg ccatgtcggc tttcggtaga tcctgataca gatgaaacgc
    21301 aatcaaccct ggcagcacca gtaccagcgg gtcgagcatt ttcagcaccg ccgttaacag
    21361 cgcccccttt tgcccttccg ccaggctttt tgacgccagc gtgcgttgca cgatgccctg
    21421 atttgtacac cagtaaaagg tgttcaccag aattaaaccg gtaaatgccg cgccaatcgg
    21481 caagggatcg gtcgggccac cgactgagtt taatttctcg gcgtgaacgg tggtaatttg
    21541 ctcaatgccc tgcataaagc tgcccttgcc catcgcgatc aggccaaata ccggcaccat
    21601 caacccgccg ataaccagcc caataccgtt gatggagtcc gccactgcca ttgcgcgcag
    21661 tccgccgatc accgcataca aaattcccgc cagaccaagc aaaattacca gtagccagat
    21721 agccgcaccg tgagaaatct gtagcgattc cccgacgtga aacaggctgt tgagcgccaa
    21781 cgcgccggag tagagaacaa tcggcagaaa gcagacgccg gtggcaatga ggaagcagaa
    21841 gtcgatgata atacgcgtcg ttttatcata acgttcctcc agaaaatcgg ggatggtggc
    21901 aatgccgcgc ttcagatagc gcggtaaaaa gattagcgcg aggaagatca gcgtcaccgc
    21961 tgaagtcact tcccagccca tcaccgacat gccgcttttg taggcctgcc cggaaagacc
    22021 aaccagttgt tccgtggaaa ggttggttaa cattaacgaa gcggcaataa ccggcgcttt
    22081 tagtgaacgt ccggcaagaa aatagccttg ttgcgatccg gtatctgttt tgcgaacctt
    22141 ccactaggtg atcaccgcca ccagcagcgt aaaaccgaca aaactcaaga tttgtaacga
    22201 attcatctca tagcccttat ttatgtttat gtcccggacg catgaatata gggagaggta
    22261 aaatgtgtcg ggtcaggcag tcagattctt aaaatacaaa cgtcgtatcc ctgaacggat
    22321 tgttttctgt taagttcagg ttgtgagcat aattcgccgg agggatatta aaatgaatgg
    22381 aaaattgcaa agttcagatg taaaaaacga aactccgtac aatattccct tattgattaa
    22441 cgaaaatgtg atctccagcg gaatttctct gatctcgctc tggcatacct acgccgacga
    22501 gcattaccgg gtgatctggc cgcgggacaa gaagaaaccg cttatcgcca actcatgggt
    22561 tgcggtgtat accgtacaag gatgtgggaa aattctttta aagaatggcg aacaaataac
    22621 tctgcatggc aactgtatta tatttttaaa gccaatggat attcactctt atcactgtga
    22681 aggtttagtc ttggaacagt actggatgga atttaccccc accagtatga tggatattcc
    22741 cgttggtcag caaagcgtta tttataatgg cgaaatttat aatcaggaac tcaccgaagt
    22801 tgctgagtta ataacttcac cagaagcaat aaaaaataat ctggcagtcg cttttctgac
    22861 gaaaattatt tatcagtgga tttgtcttat gtctgcagat ggtaaaaaag atccacaacg
    22921 gcggcaaatt gaaaaattaa ttgccacttt acatgccagt ctgcaacaac gctggagcgt
    22981 agctgatatg gctgccacga tcccctgtag cgaagcctgg ttgcgtcgtc tgtttttacg
    23041 ctataccggc aagacgccga aagaatatta cctcgatgcg cgtctggatc tggcgctatc
    23101 gctattaaag caacaaggaa actcggttgg cgaagtcgct gatacgctca acttcttcga
    23161 ctcctttcat ttcagcaaag cctttaaaca taaatttggt tatgcgccgt cagccgtgct
    23221 aaagaatacg gaccagcacc caacggatgc cagtccacac aattaacgta actctggcca
    23281 gtacgctttg ttggcctcca gcaactcatc cagaatagct ttggcgaccg aggcgctcgg
    23341 taccgttttc gacaacgtaa tcgcctgcca caggtgctga tatgaacgct gttcccaggc
    23401 gtccacgacc agtttttcca ccgccacttg ctgactcatt aaccctttct gaaactgcgg
    23461 gatatcgccg accaccagtg gttcaggccg tagtagcgca cgcgcatctg tttgcgatct
    23521 tgcagcccaa caatttgcgc catccggctt tcaataccga ttggcatgtc acagatgttg
    23581 aggattttcg cattcgggcg cagacgacgc gtggcttctg cgacaattgc tgccgggttg
    23641 gagtagttga gcatctaggc gtttggtgaa tatttttcca tataatccac cagttccagg
    23701 acgccgccaa tggaacgcat gccgtacgct attccgccgg gtccgcaagt ttcctgacca
    23761 acaacaccgt ggcgccgtgg gattttttca tccagttcgc gcatcgggta cttgccgacg
    23821 cggatgtgcg ccataacaaa atcaacgtcg ctgaacgcca cttcaggatc ggtggtgtaa
    23881 ctaaacgcaa tgtccggcgc tttttcttta aggatgactt tacaggcttc ggcaatcact
    23941 tcctgccgcg cgccatcgtt atcataaaat ttcagtgcac gaagcgggaa acggtcctga
    24001 ttcgccagga gcatcaacac gatgcctggc gtaaaggtgc ttccaccgcc tgcgacaacc
    24061 actgagaatt tggtcattat actgcctccg taatggcaac attttctgct gattgatgag
    24121 aattaattaa gctatcgagc tgttcacgca attgggatac atgcagacca atgatcacct
    24181 gaatggcatc gccactacgg aagacgccgt gcgctcccag ctttttaaag acttcgtcat
    24241 ccagcgtttg tgacatgtca tgcagtgcaa tacgtagacg cgtcgcgcaa ttgttaatgc
    24301 tggagatatt gccgaccccg cccagggctt gcaggatacc ggcagcctga tccagctctt
    24361 tttttggctc tgcagcggtg gtttagcctc gcgaggcttt gtattcggct tttgagtaga
    24421 gtttcacttc cgcatcttca cgtcccggcg tgcacatgtt gaactgcaga atcagggggc
    24481 ggaaaaccac gaagtacagc agggtaaagc acaacccaat ggcgatttgg gtcagcatca
    24541 tatccgcgtg gttgctgaac atcgggatcc agttttgcgg taaaacctgg tcaatcagac
    24601 ctccgcccat gttgcccacc acaccaaaga gatacattac ggtcgacatt gaggccgcca
    24661 gcacggcgtg taccgcaaac agcaacggtg aaatgaacag gaaggtaaat tccagcggtt
    24721 cggtaattcc caccagcatg gcggttaagg ttgcgggaat cagcaagccc gccactttta
    24781 cccgattttc cggtgcggca gtgaagtaca tcgctaaaga aatgcccacg gcaccaaaga
    24841 tttttgagtt accgtgcagg gcaaaaccgc cttccgggaa caacgatttc agcggctcgg
    24901 cgctcagact gaactcttgc agatgctgcg cccagtacat ctgaatgccg ccttcaacag
    24961 ctgccggacc aaagataaac ggtccgtaga tgaagtggtg taaaccggtt gggatcagaa
    25021 tacgttcgag gaaggtgtaa acccacaccc caagcgcacc cgccgaacgc aggaacgctt
    25081 gcagagattc aatccccatt tgtacttttg gccagccgag caatgtcagc caggcacagg
    25141 ggatcatcac caggaaggcg ataatcacca cataagacgt cccctggaaa atgccgagaa
    25201 aaaccggcag ttttttatcg aacagacggt tatgcagcgc cgtcacaatg ccggaaatga
    25261 taattgcgcc gataatgctg gtatcgaggg ttttaatccc ggccatcatt gtcagaccgc
    25321 tacctgccac cgcgtcctga gtgaaatcga cgccgaagta gcttccccag gtcattccca
    25381 tcgcattgat gaaatagttc caggtcagga aactcaccat caccgccaga caagcacgtc
    25441 cctgcgcttg cttagcaagg ccaatgggta aaccgacagc aaaaatcagc ggcatattac
    25501 ggaataccgt ccaaccgccc tcttcaataa tgtgtacgat ttgcgcgaat aaactgttcg
    25561 gatcggtcag tgattccccg acaaacatcg ggttttgcag caagatggca agacccacca
    25621 caatcccggc gaagggaaac agcagcactg gcgtgaacat cgcgccgcca aagcgttgaa
    25681 tttgactgag catttccaca tcctcttttc tcaattctgt aagtaggaag cctgattggt
    25741 tatgcggcag acctggagcg aggataggaa gatgcgatcg gcagaacatg acgacccaat
    25801 gggattcgtg atcgctttca tgcttttcgt tgggcggtag agatctacgt tttcgcgaaa
    25861 aagtagacat ctgtgagcac aggtacacag catcgaacac atctttaaaa aaaagatttt
    25921 tttcaatcga ttaagcagaa cttgtgggcg cattacccgt gcttgcaggc aaaaaagaga
    25981 gctagagatg atctacaaaa gcattgcgga gcggttaaga attcgactta attccgcgga
    26041 tttcacgcta aacggccttc ttcccggtga aaaaaagctg gcggaagagt ttgcggtatc
    26101 gcggatgact atccgtaaag ccattgacct gctggtagcg tgggggctgg tggtccgccg
    26161 ccacggcagc ggcacttacc tggtgcgcaa agatgtgctg catcaaaccg ccagcctgac
    26221 cggactggtg gaggtgttaa aacggcaggg aaaaacggtc accagccagg tgctgatttt
    26281 tgaaatcatg cctgcgcctc cggccattgc cagccagtta cggattcaaa tcaacgagca
    26341 gatctacttc tcccgtcgcg ttcgttttgt ggaagggaaa ccgctaatgc tggaagacag
    26401 ctatatgccg gtaaaactgt tccgtaacct ttcactgcaa catctggaag ggtcgaagtt
    26461 tgaatatatt gaacaagagt gcgggatttt gattggcggt aattatgaaa gcctgatgcc
    26521 ggtgctcgcc gatagactgc tggcgcggca aatgaaggta gcggaacaca cgccactgct
    26581 gcggatcacc tcgttgtcat atagcgagag cggggagttt ttgaattatt cagtgatgtt
    26641 cagaaatgcc agcgaatact aggtggagta ccatttacgg cgactccacc cggaaaagag
    26701 ttaaccgata ctccagaaga gcaccgccag taattggggg gtgataatgc gcaggaacat
    26761 cactaacgga tagacagtgg cgtaagagag cgccgccgca ccactggttg gatgaagatt
    26821 attagcaaac gccagcgccg gaggatcggt catggaacct gccagcatcc cgcacatggt
    26881 caggtaattc attttggcta acatccgcgc cagaatgcca acagtaatca gcggaacggc
    26941 ggtgatcagg gcaccataac caatccagct tagcccttcg ccattgacca gggtattcac
    27001 aaaatcccca ccagatttca gaccaacgac cgagaggaac agcacgatcc ccagctcccg
    27061 cagcgcgagg ttggcacttg gcggcataaa ccagtacagc ttgccgatac tgccgatacg
    27121 cccgaggatc aacgccataa tcagcggacc gcccgccagc cccagtttca acgcggccgg
    27181 gaatcctggc acaaagacgg gaatagaacc taacaatacg ccaagcccga tgccaataaa
    27241 cactggcagc atctgaacct gttgcagttt ttgctgcgca ttccccagca cattggcaac
    27301 ggcatcaatt gcggacggac gccccaccag gttgaggata tcgccgaact gcaggctgat
    27361 atcgccactg gcgaccagtt cgaccccggc acggttcagg cgcgagataa caacgtcata
    27421 gcgttcttta aagtgcaggt cgcgaatacg ttttccgagc acgttttcat tggtgaccac
    27481 cacacgctcg acgcgcaaat cagtgccttt cgtagacagc gaggtatcga cctcctgacc
    27541 aatcaccagt tgcgcattat gtaaatccgc tggctgcccc accagatgca gcaaatcgcc
    27601 cagttggata atggtatctg gcgaaggtac ttttagggtt tcttctcgtt tcagacgcga
    27661 gcagataatt ttgtcgccgt tgagaatcgg tacatcttta atggctaaat catgcaggtt
    27721 agggttctca acgcgaatat tgatagtctt gatcagcgcg ccgccattgg tgcgtgaaga
    27781 ctcgtgctgc tgagcttctg tctcgacatt gacgcggaaa ataacccgca acatccacat
    27841 ggtgaacaaa atcccgcaaa tgccgaatgg atacgccatc gcgtaactca tccccatctg
    27901 atcgaccatt tccattggtg tacccaggtc gcgcaagatc tgctgccctg cccccagcgc
    27961 tggcgtattg gttaccgcac cggagaaaat ccccagcact accggcagtg gaatatcaaa
    28021 cagtttatgc aggatggcgg taaccagacc accgatgatg acgatcagaa cagcaaacag
    28081 gttgaggcgt aatccggaga cgcgcaatga ggcaaagaag cccggcccta cctgaatccc
    28141 gatggtataa acgaacagga tcaggccaaa ttcctgaata acatgcagca tatcgctact
    28201 taatgtcatc cccgcctgag aaacaaaatg gccgacgatg atcccaccaa acagcacgcc
    28261 gccaatacct aatcctatgc cgcgaaattt gacgttgccg ataaacaaac cgacgactgc
    28321 caccaaagcc agaatactga ccgttaatgc tatatcactc atcgtccatt tctcttgaga
    28381 taacatttta attgctacgg attatggctg aatcggtgaa gcagatttgc cttatagcgc
    28441 acattatcac gggaaagaac gtgccgaaat atcttaaaca gtcgaaattt aacgcattgt
    28501 tgaagaagaa agacaaaaaa agccccacca gaatggcggg gcaaagagaa tagctagtta
    28561 gctatttaac gcgggacgtt cgctgatagc gatacgctgc gctgcgatgg gttctggctc
    28621 attacgaatt aaatcaatat gcagtaaacc gtttacgaag gttgcgccag agacttccat
    28681 attttcagcc agcgtaaaac tcaggctaaa tggctgattc ataagccctt gatgcagcca
    28741 ttttttctct tcttttggct gctccggcgt gccttttacg ctcaggcgcg taccttccag
    28801 ttgaatctct aaatcttcct gacggaaacc tgccagcgca agggtaatgc ggtagtggtt
    28861 atcgtcgctt ttctcaatgt tgtacggcgg gaagctctgg ctttcaccgg cgttttgcag
    28921 tgcgttggcc agtttgtcaa aaccgatcca ttgacgcatc agtggggata aatcgaagtt
    28981 acgcatagtc atttctcctt ctaagaagcg agtaagtacc tgcaaatccg aagattcgca
    29041 tatgctccct gacggcgagc atggagatgt caggccgcgc caggcggcct tagggaatta
    29101 gttgatttcg atacggcgcg gttttttcgc ttccggaatc acgcgttcga gatcgatata
    29161 cagcaaacca tttaccaggt tagccccacg aacatgaatg ttctcagcta actggaattt
    29221 gcgttcaaag ttgcgttcag cgatgccctg gtacagatag gtgcgctctt tttgttcgtc
    29281 ggcgtgagca cctttcacca ccagcagatt atcctgggcg gtaatttcca gttcgctctc
    29341 agcaaaacca gccacagcga tagcaatgcg gtaatggttt tcgtctacca gttcaacgtt
    29401 atacggaggg tagccgccat tactctggct ctggttgttt tctaagtggt taaacaaacg
    29461 gtcaaatcca atagcagaac ggtaaagcgg ggataaatca aagttacgca taatcaatag
    29521 ctcctgaaat cagcgagaat gtaagacctt ccacaatgga caggtcaggt agccagaaca
    29581 cccatcaggc gcgtcctcat cgactacgat gtaaaatggg tctggaaatg aacttttcaa
    29641 gccttatcag cggacttttt tttgcagttt atggtctatt gcatagactg agggggcagc
    29701 acgaaacgtt aaagtgcgat agccgccaca gtgcgacgta atgatgatgt tattttcagc
    29761 aagggatcgc tataactcat caggccaaca cccacgatca acagatgaat ttattatgat
    29821 aagaaatgtg ttgttagcgt tcatgatatg cagcggaatg acattactcg gaggatgctc
    29881 cagcgtgatg tcccataccg gggggaaaga aggaacgtat cccggcaccc gcgccagcgc
    29941 caccatgatt ggcgacgatg aaaccaactg gggctctaag tcgctggcga ttctcgatat
    30001 gccgtttacc gctgtgatgg acacgctttt actgccgtgg gatgtgttcc gcaaagacag
    30061 ctcggtgcgc tcgcgcgttg aaaaaagcga agccaacgcg caagccacca atgcggttat
    30121 tccaccggcc agaatgcctg ataactgatt tagcgatcgg tttcggtgat ccacagctgg
    30181 ccaccttcca tccacgccag ccattgacca tccggcgaga agaccacggc gtccgcagaa
    30241 ggtggattgg cgtggttttc ggttaaatac tcaacctcgc cgctttgcgc atgggcacaa
    30301 acaattcgat tatccagcac aaagcccaac cattctcctg acggatgcca gttaaatgca
    30361 gactgaatat ccgttttgtt atgggttaac ttgcgcggtt cgccgccctg tggcgagata
    30421 agccacagtt gcacaatgcc gttatcatca cgcattaaaa acgcgatttg cgtaccctgc
    30481 ggattacagc gcacccagtg gcgcgggacg ttgactaacc ccggataagc ccgatgatgg
    30541 gtaaaggtta aacgtcgctg cacgacgcca cgcggtggcg cgggtagggt tgtttccgtt
    30601 ccacttaacg gcgcatcacc tgccgatttc cagctagctt catcttgcgg taactcaacg
    30661 ataaatagct ccggcacttt ctcgcccttt ggcgaaagtg tgtcgccaat aaacgccagc
    30721 gcgtgatttc ctacccatcc ttcttcataa gcacgattga tttcatcact gccaggctgc
    30781 ggcgtgggcg tggttttact caccagcaca caccagtggc taccgctgta ttcacgcgga
    30841 tgttgctttt gtacgttgac cgggccaaac ggtgcagcaa ccccgacgtt tcgcaaatcc
    30901 agcgccggat cgagttgatg cattacatgg tcgttatagg taaagctccc cctttcaccg
    30961 ttcgggctaa agacatgcac atggctgcca ccgcgcagcg ctcctggcgt atacggcgcg
    31021 gtaatatcca ttgcatcgag attgctcacc ttgccgcctt cagcaatgac tccgcgtcga
    31081 tgatggaagt cgtaatgcca tgtttcatca ggattttcag ggccgtgaat aaagacatat
    31141 ttctctgact tcggatgaac ggtcaccacg ccgacgtgtg cgccctgtga cgcgcgatag
    31201 ataacctcga cctcgcctgt atggatattc acacgctcaa tggtctcgcc ggtaaacgac
    31261 gccccagaag gacgcacgtc aaataccagc cactggctgt cgggcgtcca ggtattggta
    31321 ttggtgagca ggtgattacg gggagcaaag gttatctgtt tcatggcgcg gtcctgatac
    31381 aaaacggttc ccgccatcat acttcacaag gacttcactt tcagacaata gtcttttgcc
    31441 atcgtgatga atgtgagtga aaagatggaa catttcgacg tggcgattat tggcctcggc
    31501 ccggcagggt cggcgttggc acgaaagtta gccggcaaaa tgcaggtgat cgcgctggat
    31561 aaaaagcacc agtgcggtac tgaaggtttc agcaaacctt gcagattttc agcgtcaaaa
    31621 ctgtcgacgt cgccgcatcg ctcacgcgta actaccagcg aagctatatc aatattaatc
    31681 gccatgcttt cgacttgtgg atgaaatcgc tgatccccgc cagcgttgag gtttatcacg
    31741 atagcctgtg ccgaaaaatc tggcgtgagg atgataaatg gcatgtcatt tttcgtgcag
    31801 acggctggga gcaacatatt accgcccgct atctggtcgg tgccgatggc gcaaactcga
    31861 tggtgcggcg acatctctac ccggatcatc aaatccgtaa atatgtcgct atccagcagt
    31921 ggttcgcaga gaaacatccg gtgccgttct actcctgcat ctttgataat gcgataactg
    31981 actgttactc atggagtatc agcaaagatg gttattttat ctttggcggt gcctatccaa
    32041 tgaaagacgg tcagacgcgt ttcacgacgc tgaaagagaa aatgagcgcc tttcagttcc
    32101 agtttggcaa agcggtgaaa agtgagaaat gcacggtgtt gtttccctca cgctggcagg
    32161 attttgtctg tggtaaggac aacgcctttc tgattggtga agcggcggga tttatcagcg
    32221 ccagctcgct ggaggggatt agctatgcgc tggatagcgc agagattctg cgtgcggtgt
    32281 tcctgaaaca gccggagaag agcaacgccg cttactggcg cgccacacgc aaactgcgtt
    32341 taaaactctt cggcaagata gtaaaaagcc gatgcctgac cgcaccggct ttaagaaagt
    32401 ggattatgcg cagtggtgtg gcgcatattc cacagttgaa agattatcca acgcgcttca
    32461 catcacccac cagcaggatg taagagagcg cgccaatcaa cgcgacggcg gagatataaa
    32521 ccagtgcagg cgcgaaaccg taaccctgcg ccaggtaccc caccaccagc ggaacggtga
    32581 tgccgcccag accaccggcg aagttaaaca cgccgccggt taaaccaatc aggcgcatcg
    32641 gtgccagaga agagaccagc gaccaggtaa tcgaagcaaa accgttgccg aagaatgcca
    32701 gcgccatcag gcacataatc atcatcggat cgttggtgta gttagcgccc ataatgcagg
    32761 tggagatcag caagccgcag ataatcggcg ttttacgcgc aaagcccagt gaaaagccct
    32821 tacgtaccag cagatccgcg acccagccag agagcaggac gccgacaaac gccgcgagga
    32881 atggcaccgt ggtcataaag cctgctttca gcgccgtgat tcctttttcc tgggttaaat
    32941 agttcgggaa ccaggttaag aaaaaccaca gtgcagaagc caccgcaaat tgcccaagat
    33001 aaacgccgat cagtttacga tggaacacca gtttccagtc tttggctgtt aacggctgac
    33061 gcgcctcttt cttcaccggc gcatcaccat ccaccagacc gccgccatca cgaatgtaat
    33121 ccagttcagc tttgctgata cctttggtca ggcgcggcgg ctgataaacc ttaaaccaaa
    33181 tcagcgacca gataatgccg ataccaccag tgacaatgaa cacccagtgc cagctcaaca
    33241 tctcctgaat ccagatcagc agcggagtca aaaacgccag accgacaaac tgaccagacg
    33301 tataaaaacc aacggcggaa gcgcgttcat gttccggaaa ccagctggtc accatccggt
    33361 tattggtcgg gaaagcgggc gcttcgaaaa taccggttat cgcacgcaga ccaattaatg
    33421 acattaatcc cgtggcaaag ccctggaata aagtcgccac tgaccaacca aatatcgcaa
    33481 taaaataagt cacgcgagaa cctacgcgat ctaaaaacca accgccgggg atctggcata
    33541 gcgtataaag ccaggcgaag gccgaaaata catagcccat ttccgctttc gtaatgccga
    33601 actcttcctg aatatgggcg gaagccacgg ccaggttagc gcggtcaaca taacaaatga
    33661 ccaccgtaat aaagatcatc accagcgtca gataacgccg acgccccggc tttgctgcat
    33721 taacgggaat atccatagcg agctttctcc agattttggg catagcgaag ccgctcacca
    33781 tgccctgtaa tttacagagg gttatatttt tgtattgctg ttttagtgcc cgatgagggg
    33841 cttacgtggc aggaattacc actctgctac gctgttatct tcatgacgcc agagcggatt
    33901 acgccagtcc ggggcatttt tactgaactc aataacttta gcttcgtcga tttccacacc
    33961 taagcccggt ttcgttaacg gtttaaagaa gccgccaacc atgctgaagt cttctttgtt
    34021 tttcacaaag tcgagtaact ccgcgccttt gttgtaatga atgcccatac tttgttactg
    34081 aagtaccgcg ttataggaaa caaagtcgat atgcaggcaa gccgccagtg caatcggtcc
    34141 gaacggacag tgcggcgcaa gggtcacatc ataggcttct gccattccag caattttgta
    34201 gcattcggta ataccgcctg catgggagag atccggttgc agaatcgaaa taccgcctgc
    34261 ctccagcacg cgtttaaaat cgaaacgtga gaacatgcgc tcacccgccg ccagtggaat
    34321 atgcgtttgt gccgccaatt tcgggtagta ttcggcttgt tccgccagca ccggctcttc
    34381 aataaacagc gggcgatacg gctccagttc tttaatcagc actttcgcca ttggcgcgct
    34441 gacgcgaccg tggaaatcaa gaccaaactc aatctgattg ccaaaagctt cacgaatttg
    34501 tgccacggtg ttgactgccg catctaccgc gcgggagtta tcaattagcc ccagttcttc
    34561 acaaccgttc agtttgaagg tatcgaagcc gatttcgcgc agggttttaa tgccgtcgat
    34621 aacatccgcc ggacgatcgc cgccgaccca actgtaggct ttaattttgt cgcgaaccag
    34681 gccgcccatc agttgccaga ctggcgcatt cagcactttg cctttgatat cccataacgc
    34741 ctggtcgatc ccggcgatgg cgctcatcag gattggaccg ccacgataaa atccggcgcg
    34801 atacatcact tgccataagt cgttgatgcg cgaaggatcc tgaccaatca aatagtcacc
    34861 cagctcgtga actgccgctt ccaccgtacg ggcgcggcct tcaatcacgg gctcgcccca
    34921 accgaccacg ccttcatcgg tttcaatttt caggaacatc cagcggggag gtaaacgata
    34981 cgtggtaatt ttggtgattt tcattgcact gcctctcgat acgccttaac aaatgctgct
    35041 gcctgctgcg cggtgcgttc tacggactgc ccggcgcgat aaagatcgct gcctaagccc
    35101 gccccaacac aacctgcgtc tatccactgc gccaggtttt ctggcgtcac gccgccaacg
    35161 gcaaagactg cgatgtccgg tggtaatacc acttttaacg ctttgatgta ttgcggacca
    35221 aaagccgatg acggaaatat tttcagcgcc tgcgcgcccg cttcgagcgc ggtaaaggct
    35281 tcggtcgccg tcgcgcagcc ggggcaaacg gtcatgccgt agcccaccgc gcggcggatc
    35341 acttcactat ggatattggg cgtaacgatg agctgacagc ccatcctggc gagcgcatcg
    35401 acctgttcag gtttcagtac cgtacctgcg ccaatcaacg ccttgtcgcc atacgcatca
    35461 acgatggcgg gaatgctctg ctcccattgt ggggaattca gcgggatttc aaccgcgtcg
    35521 aacccggcgt caatcaccgc gccaacatgc gccagcgcct cgtcgggcgt aatgccgcgc
    35581 aaaatggcga tcagcgggag tttagtttgc cactgcatga gcgatgctcc ttataccagc
    35641 ctgaaatgcc gtgtcgcccg ccaccgccgc cacgtcgcaa cccatcgcct gaaaggcttg
    35701 ctggtagcgc gcggtcagcg atgttccggc gacaagggtg atggcgtgtt gatgggtcac
    35761 atagtcgcgc atactggcaa cctctgcgcc aatcaacaaa ccagagagaa attcgctgac
    35821 ctgttcgcgg agaagtgttc ccagcacatg cgaggcgcga acttcaaaaa gctgcggcaa
    35881 tatggcgggc gcattaaggc cgcgctcaag gccagccgcg aaggcatcgg cagagttttc
    35941 ctgcggcggc aaacctgcgc caatcaatga gtgatttaac agtaaatgat gtaattcacc
    36001 ggtcatcacg gtgcgaaaat cgttgatttg ctggctatcg gcctgcaccc atttgcaatg
    36061 cgtaccgggc ataacataaa gagaggaagg agccagagct cgcgcgccga tcaattgtgt
    36121 ttcttcgccg cgcatcacat tgtggttatc gtcatgagag acgcataatc cgggaataat
    36181 ccagatattg tcgccaacag acgttaattg ttcgccaata gacgaaaaac gggcaggaac
    36241 agataaatac ggagcaactt tccagccgac attgctgccg accattcctg ccatcaccac
    36301 tggcgtattt tcttcacgcc agtcggtcgt gacttctgct aacaccgcag ccggagattt
    36361 tccgttcagg cgcgtgacgc ctgcttctga ttgcctgctc tccaggcagt ggtcgccctg
    36421 ataaagccag gcgcgcagat tggtcgatcc ccagtcaatt gcgatgtagc gagctgtcat
    36481 gtgatttcct ttaaccttcg tgtcgagctg gcaatcatgg taagcgccgc ctgctctgcc
    36541 gcatcgccgt cctgatgccg tatcgcatcg aacagcgcct tatgttcctg gagcgtttgc
    36601 ggcatgttgg cctcatcgcc catccaggtt cgttcaaaaa ccgcccgctg cagcgaactg
    36661 atcgcaatgc taagttgctg taacaccggg ttatgcaccg actgcagcac cgcctcgtgg
    36721 tagcgaatat ccgcttcgtt aaacgcttcg cggtcctgat tgttggcaat catctcgttc
    36781 agcgccgatt caatctgcgc cagatcgctg gaggtcgcgc gctctgctgc ccaacgggca
    36841 atcgccggtt ccaccagatt tcgcacttcg ctcatggcac tgataagccg tgggtcgtag
    36901 tcgttttcca gcacccattg cagtacgtca gtgtcgaggt aattccactg gttacgcggt
    36961 gccacaaacg ccccgcgata acgtttcatt tcaatcagcc gcttcgccat cagcgaacgg
    37021 aacacctcac ggatgatgtt gcgcgaggtt gcaaactctt cacagagctc cgcctcagcc
    37081 ggaagtggcg agccaggtac gtatttgccg tgaacgatct gtttacccag cgtaatgaca
    37141 atgcgatcgg ttttattgag agtcatggag agtccttgtg ctctgtatgt tcttctctac
    37201 tttaccccga tcgatgcata acgcggcaac tttgtagtac cagcgtgatg acgttcgcgt
    37261 ttgccgtgcg tgtaatgtag tacaaactta tattgttgtg ctacaattta gatcaaaaaa
    37321 agaacaatgt ataaaaaaat gacatgcgtc gggcagaaat ctgaaaaggg atatcaggcg
    37381 ctaaacagga gggaaagaag agtatgcttt caacggctta gctactcgtt taaaagatta
    37441 atcatgaagt tgaattttaa gggatttttt aaggctgccg gtttattccc actggcgctg
    37501 atgctttcag gctgtatctc gtatgctctg gtttcccata ccgcaaaggg tagttcagga
    37561 aagtatcaat cgcagtcaga caccatcact gggctatcgc aggcaaaaga tagtaatgga
    37621 acaaaaggct atgtttttgt aggggaatcg ctggattacc ttatcactga tggtgccgat
    37681 gacatcgtta agatgctcaa tgatccagca cttaaccggc acaatattca ggttgccgat
    37741 gacgcaagat ttgttttaaa tgcggggaaa aagaaattta ccggcacaat atcgctttac
    37801 tactactgga ataacgaaga agaaaaggca ctggcaacgc attatggttt tgcctgtggt
    37861 gttcaacact gtaccaggtc actggaaaac ctaaaaggca caatccatga gaaaaataaa
    37921 aacatggatt actcaaaggt gatggcgttc taccatccat ttaaagtgcg attttatgaa
    37981 tactattcac ccagaggcat tccggatggt gtttccgcag cattactgcc agtgactgtt
    38041 acgctggaca tcattactgc accgctgcaa tttctggttg tatatgcagt aaaccaataa
    38101 tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgccc acagattaat tcagcacata
    38161 cttctcaata gcaaacgcca cgccatcttc aaggttggat tttgtgacaa agttcgccat
    38221 ttctttcacc gaaggaatag cgttatccat cgccacaccg acgcctgcat attcaatcat
    38281 tgcgatgtcg ttttcctgat cgccaatcgc catgatttct tccggtttaa tacctaacac
    38341 gtcggccagt gatttcacac ccgtaccttt gttaacgcgt ttatcgagga tttcgaggaa
    38401 gtacggcgca cttttcagca cggtatattt ctctttcact tcctgcggaa tacgcgcgat
    38461 agcctggtcg aggatggcgg gttcatcaat catcatcact ttcaggaact gggtattggg
    38521 gtccattttc tccgcttcgc agaacaccag cggaattgtg gcaacgaagg attcatgcac
    38581 cgtgtagtag ctgatatcgc ggttagcggt gtacagcgtg gtgcggtcca gggcgtggaa
    38641 atgagaaccg acttcgcgag agagtttttc caggaaacga tagtcgtcat agctgagagc
    38701 ggtttgcgcc acggtgctac catcagcggc cttctgtacc agcgcgccgt tataagtaat
    38761 gcagtagtcg cccggctgtt ccatatgcag ctctttcagg tagttgtgca cacctgcata
    38821 cggacgaccc gtcgttagca cgacattcac gccacgggcg cgagctgcgg caatcgcatt
    38881 tttaacggcg ggtgaaatgg tgtgatcggg cagcagaagg gtgccatcca tatcgatagc
    38941 aatgagtttg atagccatga gttcccaagg tagattagtt cctgacccat gctaacgcga
    39001 ttccgctcaa aaatcagtac aacacccgag ggaaaagggg atgcaacgcg ggttgcatgc
    39061 tccccatttg cttagcggaa gagtttccct ttcagcagtt ccatgcctgc ggaaagcaga
    39121 tcgttattgg cttgtgctga cacttcacct tgcggtgaga gcgcatcaat aatcttcggc
    39181 aattgctctg ccagtaaact ggaagccgta ctggtatcca cgccaagttt ttgcccgaga
    39241 tcggacaccg catttgtgcc gagcgccgat tccagttgct cgccgctaac tgattgattt
    39301 ctctgttgat tactcaacca agttgagaga atggccccta agccgccact ttgcagtttt
    39361 tccagcagca cctgaatgcc accctgctcc tcaacccaac ttaaaatcgc ctgatatttc
    39421 cccgcatcgc ctttcagaaa ggcaccgaca acttcatcaa aaagccccat gataatcacc
    39481 tgtaaagcgt tacgtgttga cccaaaaagt atagatctgc gggatgataa ttgcggattg
    39541 cagaaataaa aagggcggag atgatctccg cccttttctt atagcttctt gccggatgcg
    39601 gcgtgaacgc cttatccggc ctacaaaatc atgcaaaatc aatatattgc aggagtctcg
    39661 taggactaat aagcgtagcg catcaggcac gctcgcatgg ttaacgccat taaatatcaa
    39721 tattcgccgc tttcagggcg ttctcttcaa taaacgcacg acgcggttct acggcgtcgc
    39781 ccatcagcgt ggtgaacaac tggtcagcgg caatcgcatc cttaacggta acgcgcagca
    39841 tacgacggct ttccgggtcc atggtggttt cccacagctg ttccgggttc atctcgccca
    39901 gacctttata acgctggatg gagaggccac gacgggactc tttcaccagc cagtccagcg
    39961 cctgctcgaa gctggctacc ggctgacgac gctcgccacg ttcgataaac gcatcttctt
    40021 ccagcaagcc acgcagtttc tcacccagcg tgcagatacg acgatattcg ccaccggtga
    40081 taaactcgtg atccagcgga tagtcagtat ccacaccgtg ggtacgcacg cgaacaatcg
    40141 gctcgaacag gttttgttcg gcattggtgt ggacatcaaa cttccactgg ctgccgtgct
    40201 gttctttgtc gttcagttcg ctgaccagcg cgttcaccca gcgggtaacg gtctgctcat
    40261 cagaaaggtc agcttccgtc aacgtcggct gatagataag ctctttcagc attgctttcg
    40321 gataacgacg ctccatgcgg ttgatcattt tctgcgtcgc gttgtactca gataccagtt
    40381 tctctaacgc ttcgccagcc aatgccggtg cactggcgtt ggtgtgcagc gttgcgccgt
    40441 ccagcgcgat agagatctgg tactgatcca tcgcttcgtc gtctttaatg tactgttcct
    40501 gcttgccttt cttcactttg tacagcggcg gctgagcgat gtagacgtga ccgcgttcaa
    40561 cgatttccgg catctgacga tagaagaagg tcaacagcag cgtacgaatg tgcgagccgt
    40621 cgacgtccgc atcggtcatg atgatgatgc tgtgataacg cagtttgtcc gggttgtact
    40681 cgtcacgacc gataccacag ccaagcgcgg tgataagcgt cgccacttcc tgagaagaga
    40741 gcatcttatc gaagcgcgct ttctcgacgt taaggatttt acccttcagc ggcagaatcg
    40801 cctggttctt gcggttacgc ccctgcttcg cagagccgcc cgcggagtcc ccttccacca
    40861 ggtacagttc ggaaagcgcc ggatcgcgtt cctggcagtc tgccagtttg cccggcaggc
    40921 cagctaagtc gagcgcacct ttacggcggg tcatttcacg cgcgcgacgc gccgcttcac
    40981 gggcacgggc agcatcgata attttgccaa ccacgatttt cgcatcagtt gggttttcca
    41041 gcaggtattc cgccagcagt tcgttcatct gctgttcaac cgccgatttc acctcagaag
    41101 aaaccagttt gtctttggtc tgggaggaga atttcgggtc cggcactttt acggaaacga
    41161 ccgcaatcag gccttcacgc gcatcgtcac cggtggcgct gactttggct tttttgctgt
    41221 agccttcttt gtccatgtag gcattcaggg tacgggtcat cgccgcacgg aagcctgcca
    41281 ggtgagtacc gccgtcacgc tgcggaatgt tgttggtaaa gcagtagatg ttttcctgga
    41341 agccatcgtt ccactgcagc gccacttcaa cgccaatacc gtctttttcg gtggagaagt
    41401 agaagatatt tgggtggatc ggcgttttgt tcttgttcag atattcaacg aacgctttga
    41461 tgccgccttc atagtggaag tggtcttctt tgccgtcacg cttgtcgcgc agacgaatgg
    41521 aaacgccgga gttgaggaac gacaactcac gcagacgttt cgccagaatt tcatattcga
    41581 actcggtcac attggtgaag gtttcgaggc tgggccagaa acgcaccatg gtgccggttt
    41641 tttcagtctc gccggtaacc gccagcggag cctgcggtac accgtgttcg tagatctgac
    41701 ggtgaatttt accctcgcgc tgaataacca gctccagttt ttgcgacagg gcgtttacta
    41761 ccgaaacacc aacgccgtgc agaccgccgg acactttata ggagttatcg tcaaatttac
    41821 cgcctgcgtg cagaacggtc atgatcactt ccgccgccga tacgccctct tccgggtgaa
    41881 taccggtcgg aatgccgcgc ccgtcatcct gtacagagac agagttatcg gcgtgaatgg
    41941 tgacgataat ttctttacag tgacccgcga gcgcttcgtc gatagcgtta tctaccacct
    42001 cgaataccat gtggtgcaga ccggtgccgt catccgtgtc gccgatatac atacccgggc
    42061 gcttacgcac cgcatccagc cctttcagga ctttgatact ggaggagtca taagaattcg
    42121 acatcaacgt ttctcgctca tttatacttg ggttaatccg ttattttacc cttttccacg
    42181 gtaaacatct tcgaattttc gtccgacatg tctataacgt gttcagcact gatcgcgctg
    42241 acaaagacct gtgattgcgt cgcttttaag cggctggcaa gcaacccgcg acgctcatca
    42301 tcaagctcag aggcaaaatc atctatcagg tagagacacc gccgcccgct ttcacgagtg
    42361 aggaactctc cttgcgccag acgtaaggcg cacatcaaca gcttaagctg cccacgcgat
    42421 aaggtatctt ccaccggcgc accgtcggcg cgaatgcgta agtccgcttt atgcgggcca
    42481 tgcgcggtgt aggttagctg gcgatcgcgt tcaaaattac gttccagcac ctcagcgtac
    42541 tctgtctctt tctcccagcc gcgctggaaa gagaaagtca gagaaaactc agggagaaat
    42601 tgcttacagg tatcatccat atcggccgcg ataccggcgc tatactccgc gcgccaggtg
    42661 ctgatttgct ccgccagcgg gatcagttct ttatcccacg ggcgtagctg ttcgtaacga
    42721 gtcacctggc gcagcgccgc attgcgctgc ttgagcaatc gcttgagatt gctccaggcg
    42781 gtgaaaaatc taggttcgtt gtgaaagcat ccccagtcga ggaatgctct tctgtatttg
    42841 gggccgccgt tgagtaaagt aaacccttct ggcgttatca actgcatcgg catcaggtgc
    42901 gccagttccg cgactttatg cccgtccgta ccgtcaatac ggactttgct gtcgccctgt
    42961 ttgtctttgg ttaagccaat cgccgtctcg cgctcttcgc cctgtaatcg cccgtggaga
    43021 acaaatgcct cctgctcatg gcgaatgacg cgaccaatct gcaaactgcg aaacgcccga
    43081 ccatggccga gcgtatagat ggcttccagc acgctggttt tgccactgcc gttggcacct
    43141 accagaaagt taaagccggg agataaagcg agatccgcgg tttcaatatt gcggaaatcg
    43201 cggatcaaca agcgggtgag ggacattaca gtctcattgg catgacaaca taggcagccg
    43261 actgtgatgc ggcatcttca atctgcacgc tggaaaccga atcggtcagc atcatgcgga
    43321 cgttttcgca tttcagcgcg ttcagaacat ccagcacata gctgacgttg aagccgattt
    43381 ccatctccgc accgctatag gtaacgtcga ggatctcttc cgcttcttcc tgttccgggt
    43441 tgttggcagt aattttcagc tggttttcgc tgacatacag gcgcacaccg cggaatttct
    43501 cgttagagag aatcgccgcg cgagcaaacg cctgcttgag cagatcgcag cctgcttcca
    43561 gatgtttgtc cgggttcttc ggcagaacgc gacggtaatc cgggaagcga ccatccacca
    43621 gtttcgaggt gaaaataaag tcgccaacgt gggcacgaat gttattgctg ccaatctgca
    43681 cacgcagcag attgtcgccg ccgtcgagca tacgcatcag ttcaatcacg cctttacgcg
    43741 gtacgatcac cgaatggctt ggcaaagatt gaccaattgg cattgaacag accgccagac
    43801 ggtggccgtc ggttgccacg gtgcgcagtt cttcaccttc ggtttcaaac agcataccgt
    43861 ttaagtaata gcgaacatcc tgatgcgcca tcgaaaactg ggttgcttca atcagacgct
    43921 tcatcgtcgc ctgcggcagg gtaaattcga cttcgctctg ccagtcatcg aggttcggga
    43981 aatccgccgc tggcagggta gacagcgaaa aacggctacg cccggagcgc accagcatcc
    44041 gttcaccttc cagctgcacg gcaatttccg cgccttcagg cagaccacgg cagatatcaa
    44101 agaatttgcg cgccggaacg gtcgtcgctc ctggctcgtg tggctgaacc agcgcaacgc
    44161 gtgccaccat ttccatctcg agatcagtac cggtcagcga caacgtaccg tcagcaacct
    44221 gtaacagcag attaccgaga atcggtagcg taggacgacc acctaacgga ccgctcacct
    44281 gttgtagcgg ttttaataaa tgctcacgtt ctacggtaaa tttcataggt ttacgatgac
    44341 aatgttctga ttaaatttga aaaatcttct ttgatatcgt ggctctcttc acgcaactgc
    44401 tcgatcttac ggcaggcatg aagcaccgtc gtgtggtcac gcccaccaaa cgcatcgcca
    44461 atctctggca gactgtggtt agtcagctct ttcgctagcg ccatcgccat ctggcgcgga
    44521 cgcgccaccg agcgggatcg acgcttggaa aggagatccg cgactttgat cttgtagtac
    44581 tccgccaccg tcttctgaat attgtcgatg gtgaccagtt tttcctgcaa tgccagcaag
    44641 tcgcgcagcg cctcacgcac gaagtcgatg gtgatcgccc gtccggtaaa gttggcgtta
    44701 gcaataacgc ggttcagcgc cccttccagc tcacgtacgt tagatcgtag acgcttggcg
    44761 ataaagaacg ccacttcacc cggcaaacga atgtcatttt cgtcggcctt tttcatcagg
    44821 atcgccacac gggtttccag ctctggcggt tcgatcgcca cagtcagtcc ccaaccgaag
    44881 cgggatttca aacgatcctc aacgccgttg atctctttcg gatagcgatc cgaggtgaga
    44941 atgatctgtt gattaccttc cagcagggcg ttgaaggtgt ggaaaaactc ttcctgagat
    45001 cgttctttat tagcaaaaaa ctgaatatcg tcgatcagca gtgcatctac ggaacggtag
    45061 tagcgtttaa actcttcgat cgcgttgttt tgcagggctt taaccatgtc ctgaacaaag
    45121 cgctcggagt gcatataaac cactttggca ttcggcttgc gcgccataat gccgttaccc
    45181 accgcatgca gcaggtgagt tttacccaga cccgtgccgc cataaaggaa caacgggtta
    45241 taggcaccgc cagggttatc cgccacctgg cgagccgccg cgcgcgccag ttggttagat
    45301 ttaccttcaa cgaagttatc aaacgtgtgt ttgacgttta cgttagaacg ataggtcggt
    45361 tctgccgggg ccgggacgtt atcccaacct gagcgcgtag aaggcgcagc acgttgcggc
    45421 tgcgtttgcg ccacctgtgc aggggccgcg acgttgctcg tcactgccgc ttgtggcgtt
    45481 tgcgtcaccg gtttggtgcc gacttcaaaa cgcagctgtg gggcatccgc tccgcagaaa
    45541 ctggttagca gtccattgat attattaagg tacttgtccc gtacccaatc gaggacaaaa
    45601 cggtttggcg cgtacaggga cagcgtgtta tcgctcagtt ccgcctgcaa tgggcgtatc
    45661 cacatactga attctgtggc tggtaactca tcctgcaatc gggcaagaca ctgctgccaa
    45721 agcgaaagtg acacggcgga ctccactcga acaaaagtcg ataatgacta aggctgaaac
    45781 attcatgatt gttgacgtac gtcgaaaaga ccctgcttga gggtgacgca cgaaccgctg
    45841 tctgcggtta tatgcccgat caagatcctg caaaacgatc gggaccgcgg atcatagcct
    45901 aaactgcgca agagatcttc tgtttctcac agattttccc tatttatcca caggactttc
    45961 cagaactcgc taagtgtaaa cgatcctgcc gcgaggcggg cacgatttac gccgcatatt
    46021 ggaaaattta atgaccatag acaaaaattg gcttaatcga tctaataaag atccaggacg
    46081 atccttgcgc tttacccatc agcccgtata atcctccacc cggcgcgcca tgctggtttc
    46141 cactggtgtg aggccgtaca ttttccctgc gaaaaggtgc ggaaaagcgc ggtaaataag
    46201 gaaagagaat tgactccggt gtgtacaatt attacaatcc ggcctcttta atcacccatg
    46261 gcttcggtgt ccatcgtttc atttttcggc ggatatccaa taaagccatt gaatttattc
    46321 aagtttaggt agaaatcgcc atgaaacgca cttttcaacc gtctgtactg aagcgcaacc
    46381 gttctcacgg cttccgtgct cgtatggcta ctaaaaatgg tcgtcaggtt ctggcacgtc
    46441 gtcgtgctaa aggccgcgct cgtctgaccg tttctaagta ataaagctaa cccctgagtg
    46501 gttaagctcg catttcccag ggagttacgc ttgttaactc ccagtcaatt cacattcgtc
    46561 ttccagcagc cacaacgggc tggcacgccg caaattacca ttctcggccg cctgaattcg
    46621 ctggggcatc cccgtatcgg tcttacagtc gccaagaaaa acgttcgacg cgcccatgaa
    46681 cgcaatcgga ttaaacgtct gacgcgtgaa agcttccgtc tgcgccaaca tgaactcccg
    46741 gctatggatt tcgtggtggt ggcgaaaaaa ggggttgccg acctcgataa ccgtgctctc
    46801 tcggaagcgt tggaaaaatt atggcgccgc cactgtctcc tggctcgcgg gtcctgatag
    46861 ccctcattcg ggtctatcaa cgcctgatta gtccgctact cgggccgcat tgtcgtttca
    46921 ctccaacctg ttcaagctac ggaattgagg cattgcgcag gtttggagtg ataaaaggca
    46981 gttggttgac ggtgaaacgc gtattaaaat gccacccttt acaccctggt ggtgacgatc
    47041 ccgtcccgcc cggaccattt gataccagag aacactaacg atggattcgc aacgcaatct
    47101 tttagtcatc gctttgctgt tcgtgtcttt catgatctgg caagcctggg agcaggataa
    47161 aaacccgcaa cctcaggccc aacagaccac gcagacaacg accaccgcag cgggtagcgc
    47221 cgccgaccag ggcgtaccgg ccagtggcca ggggaaactg atctcggtta agaccgacgt
    47281 gcttgatctg accatcaaca cccgtggtgg tgatgttgag caagctctgc tgcctgctta
    47341 cccgaaagag ctgaactcta cccagccgtt ccagctgttg gaaacttcac cgcagtttat
    47401 ttatcaggca cagagcggtc tgaccggtcg tgatggcccg gataacccgg ctaacggccc
    47461 gcgtccgctg tataacgttg aaaaagacgc ttatgtgctg gctgaaggtc aaaacgaact
    47521 gcaggtgccg atgacgtata ccgacgcggc aggcaacacg tttaccaaaa cgtttgtcct
    47581 gaaacgtggt gattacgctg tcaacgtcaa ctacaacgtg cagaacgctg gcgagaaacc
    47641 gctggaaatc tcctcgtttg gtcagttgaa gcaatccatc actctgccac cgcatctcga
    47701 taccggaagc agcaacttcg cactgcacac cttccgtggc gcggcgtact ccacgcctga
    47761 cgagaagtat gagaaataca agttcgatac cattgccgat aacgaaaacc tgaacatctc
    47821 ttcgaaaggt ggttgggtgg cgatgctgca acagtatttc gcgacggcgt ggatcccgca
    47881 taacgacggt accaacaact tctataccgc taatctgggt aacggcatcg tcgctatcgg
    47941 ctataaatct cagccggtac tggttcagcc tggtcagact ggcgcgatga acagcaccct
    48001 gtgggttggc ccggaaatcc aggacaaaat ggcagctgtt gctccgcacc tggatctgac
    48061 cgttgattac ggttggttgt ggttcatctc tcagccgctg ttcaaactgc tgaaatggat
    48121 ccatagcttt gtgggtaact ggggcttctc cattatcatc atcaccttta tcgttcgtgg
    48181 catcatgtac ccgctgacca aagcgcagta cacctccatg gcgaagatgc gtatgctgca
    48241 gccgaagatt caggcaatgc gtgagcgtct gggcgatgac aaacagcgta tcagtcagga
    48301 aatgatggcg ctgtacaaag ctgagaaggt taacccgctg ggcggctgct tcccgctgct
    48361 gatccagatg ccaatcttcc tggcgttgta ctacatgctg atgggttccg ttgaactgcg
    48421 tcaggcaccg tttgcactgt ggatccacga cctgtcggca caggacccgt actacatcct
    48481 gccgatcctg atgggcgtaa cgatgttctt cattcagaag atgtcgccga ccactgtgac
    48541 cgacccgatg cagcagaaga tcatgacctt tatgccggtc atcttcaccg tgttcttcct
    48601 gtggttcccg tcaggtctgg tactgtacta tatcgtcagc aacctggtaa ccattattca
    48661 gcagcagctg atttaccgtg gtctggaaaa acgtggcctg catagccgcg agaagaaaaa
    48721 atcctgattc ggtgagtttt cgctaaaata agggcggtca gttgaccgcc ttttttcttt
    48781 tcgtagggcg gataagcacc gcgcatccgc cacacaaagc aacaggaaca tcatgagcga
    48841 taatgacact atcgtagccc aggccacacc tccgggacgt ggcggcgttg gcatcctgcg
    48901 catctccggc ttcaaagccc gtgaagttgc cgaaaccgtg ctgggtaaac tgcctaagcc
    48961 gcgctacgcc gattatcttc cgtttaaaga cgccgacggc agcgtgctcg atcaggggat
    49021 tgcgctatgg ttccctggcc cgaactcgtt caccggcgaa gatgtgctgg aactgcaagg
    49081 tcatggcggt ccggtgatcc tcgacctgct gttaaaacgc attctgacca ttcccggcct
    49141 gcggattgct cgccctggtg agttttccga acgcgcgttt cttaacgata aacttgactt
    49201 agcccaggcc gaggcgattg ccgatcttat cgacgccagt tcggaacagg cggcccgttc
    49261 ggcacttaac tcgctgcaag gcgcattctc cgcacgggtt aatcatctgg tagaagccct
    49321 cacccacttg cgcatttacg tcgaagcggc aattgatttc cctgatgaag agatcgattt
    49381 cctctccgac ggcaaaattg aagcccagct taatgacgtg atggccgatc tcgatgcagt
    49441 gcgtgctgaa gcacgtcagg gtagtttgtt gcgcgaaggg atgaaagtag tgattgccgg
    49501 acgtcctaat gccggtaaat cgagcctgtt aaacgcgctg gcgggccgtg aagcggcaat
    49561 cgtaaccgat atcgccggaa ccacgcgtga cgtgctgcgt gagcatatcc acattgacgg
    49621 aatgccgctg catatcatcg acactgccgg gctacgtgag gccagtgacg aagtggaacg
    49681 tattggtatc gagcgcgcgt ggcaggaaat tgaacaggcc gaccgcgtgc tgtttatggt
    49741 cgatggcacc acgacagacg ccgttgatcc ggcagagatc tggccggaat ttatcgcccg
    49801 tctgcccgcg aaactgccga tcaccgtggt acgcaataaa gccgatatca ccggcgaaac
    49861 gctgggaatg agtgaagtga acggtcacgc gttaattcgt ctctcggcaa ggactggtga
    49921 aggcgtggac gtgctgcgta accatctcaa acagagcatg ggctttgaca ccaatatgga
    49981 aggcggcttc ctggcgcgcc gtcgccactt acaggcgctg gaacaggcag cggagcattt
    50041 gcagcaaggt aaagcgcaac tgttgggagc ctgggcaggt gaactgctgg cggaagagtt
    50101 gcgcctggca cagcagaact taagcgaaat caccggggaa tttacttcag acgacctgct
    50161 ggggcggatt ttctccagct tctgtattgg taagtaaccg cgcttacgaa gccgtattct
    50221 gactgtcaga tgcggcttcg cttcattgtt agcactcctg ttatgcctca accctttttt
    50281 aaacattaaa
//
LOCUS       DAJTNM010000016        43207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTNM010000016 DAJTNM010000000
VERSION     DAJTNM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.016"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..504)
                     /locus_tag="OO172_001045"
     CDS             complement(<1..504)
                     /locus_tag="OO172_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5511311.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVV"
     gene            complement(644..1831)
                     /gene="metC"
                     /locus_tag="OO172_001046"
     CDS             complement(644..1831)
                     /gene="metC"
                     /locus_tag="OO172_001046"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5511312.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            2083..2817
                     /gene="exbB"
                     /locus_tag="OO172_001047"
     CDS             2083..2817
                     /gene="exbB"
                     /locus_tag="OO172_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5511313.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            2824..3249
                     /gene="exbD"
                     /locus_tag="OO172_001048"
     CDS             2824..3249
                     /gene="exbD"
                     /locus_tag="OO172_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5511314.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3521..4406)
                     /gene="yghA"
                     /locus_tag="OO172_001049"
                     /pseudo
     CDS             complement(3521..4406)
                     /gene="yghA"
                     /locus_tag="OO172_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
     gene            4597..5091
                     /locus_tag="OO172_001050"
     CDS             4597..5091
                     /locus_tag="OO172_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5511315.1"
                     /translation="MERFFENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            complement(5131..6171)
                     /gene="gpr"
                     /locus_tag="OO172_001051"
     CDS             complement(5131..6171)
                     /gene="gpr"
                     /locus_tag="OO172_001051"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5511316.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            6328..7215
                     /locus_tag="OO172_001052"
     CDS             6328..7215
                     /locus_tag="OO172_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5511317.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            7334..7621
                     /locus_tag="OO172_001053"
     CDS             7334..7621
                     /locus_tag="OO172_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5511318.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            7810..8928
                     /gene="hybO"
                     /locus_tag="OO172_001054"
     CDS             7810..8928
                     /gene="hybO"
                     /locus_tag="OO172_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5511319.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            8931..9917
                     /gene="hybA"
                     /locus_tag="OO172_001055"
     CDS             8931..9917
                     /gene="hybA"
                     /locus_tag="OO172_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5511320.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            9907..11085
                     /gene="hybB"
                     /locus_tag="OO172_001056"
     CDS             9907..11085
                     /gene="hybB"
                     /locus_tag="OO172_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5511321.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            11082..12785
                     /gene="hybC"
                     /locus_tag="OO172_001057"
     CDS             11082..12785
                     /gene="hybC"
                     /locus_tag="OO172_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5511322.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            12785..13279
                     /locus_tag="OO172_001058"
     CDS             12785..13279
                     /locus_tag="OO172_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5511323.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLSDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            13272..13760
                     /gene="hybE"
                     /locus_tag="OO172_001059"
     CDS             13272..13760
                     /gene="hybE"
                     /locus_tag="OO172_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5511324.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            13753..14094
                     /gene="hypA"
                     /locus_tag="OO172_001060"
     CDS             13753..14094
                     /gene="hypA"
                     /locus_tag="OO172_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5511325.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            14228..14476
                     /gene="hybG"
                     /locus_tag="OO172_001061"
     CDS             14228..14476
                     /gene="hybG"
                     /locus_tag="OO172_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5511326.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(14599..15465)
                     /gene="yghU"
                     /locus_tag="OO172_001062"
     CDS             complement(14599..15465)
                     /gene="yghU"
                     /locus_tag="OO172_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5511327.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            15670..17529
                     /gene="gss"
                     /locus_tag="OO172_001063"
     CDS             15670..17529
                     /gene="gss"
                     /locus_tag="OO172_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5511328.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            17821..19320
                     /gene="pitB"
                     /locus_tag="OO172_001064"
     CDS             17821..19320
                     /gene="pitB"
                     /locus_tag="OO172_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5511329.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(19369..20061)
                     /locus_tag="OO172_001065"
     CDS             complement(19369..20061)
                     /locus_tag="OO172_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5511330.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            20235..20948
                     /locus_tag="OO172_001066"
     CDS             20235..20948
                     /locus_tag="OO172_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5511331.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            20980..21738
                     /locus_tag="OO172_001067"
     CDS             20980..21738
                     /locus_tag="OO172_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511332.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            21784..23133
                     /locus_tag="OO172_001068"
     CDS             21784..23133
                     /locus_tag="OO172_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5511333.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            23133..23957
                     /locus_tag="OO172_001069"
     CDS             23133..23957
                     /locus_tag="OO172_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511334.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            23969..24529
                     /locus_tag="OO172_001070"
     CDS             23969..24529
                     /locus_tag="OO172_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5511335.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLSNE"
     gene            24560..25630
                     /gene="lptF"
                     /locus_tag="OO172_001071"
     CDS             24560..25630
                     /gene="lptF"
                     /locus_tag="OO172_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5511336.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPWQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            25627..26706
                     /gene="lptG"
                     /locus_tag="OO172_001072"
     CDS             25627..26706
                     /gene="lptG"
                     /locus_tag="OO172_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5511337.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(26742..27914)
                     /locus_tag="OO172_001073"
     CDS             complement(26742..27914)
                     /locus_tag="OO172_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5511338.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(27914..28162)
                     /locus_tag="OO172_001074"
     CDS             complement(27914..28162)
                     /locus_tag="OO172_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5511339.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            complement(28195..29109)
                     /locus_tag="OO172_001075"
     CDS             complement(28195..29109)
                     /locus_tag="OO172_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5511340.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(29106..30794)
                     /locus_tag="OO172_001076"
                     /pseudo
     CDS             complement(29106..30794)
                     /locus_tag="OO172_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            31198..32340
                     /locus_tag="OO172_001077"
                     /pseudo
     CDS             31198..32340
                     /locus_tag="OO172_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(32464..33234)
                     /gene="glcC"
                     /locus_tag="OO172_001078"
     CDS             complement(32464..33234)
                     /gene="glcC"
                     /locus_tag="OO172_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5511341.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            33480..34979
                     /gene="glcD"
                     /locus_tag="OO172_001079"
     CDS             33480..34979
                     /gene="glcD"
                     /locus_tag="OO172_001079"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HCR5511342.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANELGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            34979..36031
                     /gene="glcE"
                     /locus_tag="OO172_001080"
     CDS             34979..36031
                     /gene="glcE"
                     /locus_tag="OO172_001080"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5511343.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFCYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            36042..37265
                     /gene="glcF"
                     /locus_tag="OO172_001081"
     CDS             36042..37265
                     /gene="glcF"
                     /locus_tag="OO172_001081"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HCR5511344.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            37270..37674
                     /locus_tag="OO172_001082"
     CDS             37270..37674
                     /locus_tag="OO172_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HCR5511345.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            37696..39867
                     /gene="glcB"
                     /locus_tag="OO172_001083"
     CDS             37696..39867
                     /gene="glcB"
                     /locus_tag="OO172_001083"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5511346.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGIVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            40222..41904
                     /gene="glcA"
                     /locus_tag="OO172_001084"
     CDS             40222..41904
                     /gene="glcA"
                     /locus_tag="OO172_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HCR5511347.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFAGVIGVITLLQAYVFTGMLVS"
BASE COUNT        10340 a        10621 c        11509 g        10737 t
ORIGIN      
        1 taccacgctg acccacagca atccacttaa ccagttgaaa aactggaagc ggcggtttgg
       61 cagaccggaa attcccgcca tggttggcag cagcgtacgg acaaatgcaa gaaaacgtcc
      121 agccagcagc gccagcagac cgtggcggtc aaacatgcag gtggcgcgct ggtgatattt
      181 agcaggaagc tgtgccagcc agcctttcac cgttttggta ttccctaacc agcgcccctg
      241 aatataactt agccagcagc ccagacttgc tgcggcggtc agaatcgcaa tcgtaggcag
      301 aaaatccata accccttggg caatcaatgc gcctgccagt atcaacaagc tgtcgcctgg
      361 caaaaatgag gcgggcagca ggccgttttc taaaaacagc gtggcaaaca tgacaaagta
      421 aacaacgcta acaatatgag gatccgccag cgcggcaaag tcgtgttgcc agagcgcagc
      481 gatgatatct tgaataacag ccatggactt tcctgtggaa cagcgtttaa gcttttatct
      541 gcctattgta ctcctgaatc gtccgggacg ccttgatccc ggacgcaaca atttaagact
      601 tttcacaata aaatgtctgc aaaattgtcc aaaagtggca atgttataca atccgtgcaa
      661 aaccggcgtc cagatcggca atcagatcgt cgacatcttc cagaccaata tgcaggcgaa
      721 tcaaggtccc gctaaaatcg atctcgcctt gtgggcgaat ggcggcgata tgttctggtt
      781 gatttgccag gatcaacgat tcatacccgc cccacgagta ggccatgctg aataaactga
      841 agttatccag atagttcgcc agctcttcat cattgagttt tttcttaagc acaaaggaaa
      901 atagcccgct gctgcctgta aagtctcgtt tccagaattc gtgtccttta ctgccaggca
      961 gggcagggtg gttaactcgc gcaacttgcg gatgttctgc cagccattcg gccactttca
     1021 gactgctttc atgatgttga cgcaaacgca cacctaatgt gcgcaggcca cggctggtta
     1081 tataggcggt atcggcatcg accatctggc ccatcagata ggcattttcc cgtagctgct
     1141 cccagcaacg ggcattgcac acggcagtgc caatcatcgc atctgaatgc ccaaccagat
     1201 atttggtggc ggcctgaata gaaacatcga tgccaaaatc cagcgcctta aacagcacac
     1261 cggctgccca ggtgttgtcg atcataatga tggcatccgg caccacactg cgcacggcgg
     1321 caacaatcgc cggaacgtcg tggacttcca tagtgatgga acccggcgat tccagaaaca
     1381 cgattttagt gtttggctgc agatgcttaa cgatatcggc accaatcagc ggatcaaacc
     1441 acgatgtcgt tacgcccagt ttgctgagga ttttgctaca gaaatcctga ctcggttcat
     1501 aggcggtgtt ggtcatcaac acatgatcgc cctgttcgac aaaagcaaga atcgaattag
     1561 caaccgctgc cgccccgcag ggaaatagcg cgcagcctgc gccaccttcc agttcacaca
     1621 tcgcttgttg taaggaaaaa tgggttaacg ttccgcgccg tccatagaac aactcgccat
     1681 tggcgcgatt gcgcgtcgcg tgctttttgg cttccatact ctcaaatacc agcgaagaag
     1741 cgcgctgaat cacgctattt accgcgccaa gagtgtattt tttgctgcgt cctgcattca
     1801 ccagttgagt atcaagcttt ttgtccgcca tgtcgggatt cctgttttta tacgtctgga
     1861 tgtctaaact agcatgaata ttcgcgggcg catcctgaag aattggcata aagcagaaaa
     1921 ttttcgcaaa tggcgtgctg gtgacttttt tactgcgtaa acgcaaggaa aagaaagcaa
     1981 aggtacggca gtatgcaaat agtaatgaga acgactatca attcgacgtc gttttgatat
     2041 tattatgcgc agattttgtg acttgcgtcc tggagataca cagtgggtaa taatttaatg
     2101 cagacggacc tttccgtctg gggtatgtat cagcacgccg atattgtcgt taagtgcgtg
     2161 atgattgggc ttattttggc ctccgtagtc acctgggcaa tcttcttcag taagagcgta
     2221 gagttcttca atcagaagcg tcgccttaag cgcgagcagc aactgctggc tgaagcacgt
     2281 tccttaaacc aggccaacga tatcgccgct gattttggta gcaaaagctt aagcctgcat
     2341 ttgctcaatg aagcgcagaa tgagctggaa ctgtcagaag gcagcgacga taacgaaggt
     2401 attaaagaac gtaccagctt ccgtctggag cgtcgggtcg ccgcagtggg tcgtcaaatg
     2461 ggccgcggta acggctatct ggcaaccatc ggcgcgattt ctccgttcgt tggtctgttt
     2521 ggtacggtct ggggcatcat gaacagcttt attggtatcg cgcaaacgca gaccactaac
     2581 ctggcagtcg ttgcgccggg tatcgcagaa gctctgttag caacggcaat cggcctcgtg
     2641 gcagcgattc ctgcggtcgt tatctataac gtatttgcac gccagattgg cggctttaaa
     2701 gcgatgctgg gtgatgttgc agcgcaggta ttgttgctgc aaagccgtga cctggatctg
     2761 gaagccagcg ccgctgcgca tccggttcgt gtcgcacaaa aattacgcgc aggataatgt
     2821 ccgatggcaa tgcatcttaa cgaaaacctc gacgataacg gcgaaatgca tgatatcaac
     2881 gtgacgccgt ttatcgacgt gatgttggtt ctgctgatta tctttatggt ggcggcaccg
     2941 ttagcgacgg tagatgtgaa ggtgaacttg cctgcttcta ccagcacgcc gcagccgcgc
     3001 ccggaaaaac cggtttatct gtcggtgaag gcagacaact cgatgtttat cggtaacgat
     3061 ccggtcaccg atgaaacaat gattacggcg ttgaatgcgt taaccgaagg caagaaagac
     3121 accaccatct tcttccgagc ggacaaaacc gtcgattacg agacgttgat gaaggtaatg
     3181 gatacgctgc atcaggcggg ttacctgaag ataggtctgg tcggcgaaga aaccgccaaa
     3241 gcgaagtaaa gtagaattgc ctgatacgct acgctcatca ggcctacaaa atctattgca
     3301 acatgttgaa tcttcgtgcg tttgtaggcc ggataaggcg ttcacgacgc atccggcatt
     3361 aggtgctcaa tgcctgatgc gctacgctta tcaggcctac aaaatctatt gcaacatgtt
     3421 gaatcttcat gcgtttgtag gcggataagg cgttttcgcc acatcaggta agagtgaatt
     3481 cacaatgatg cccggttgct tttcacaacc gggcattttt ttaacctaaa tgctcgccgc
     3541 cgcacacgcc gtgcacttct gcggtgacgt agctcgactc ctgacttgcc agataaacat
     3601 atacaggggc cagttccgcc ggttgccccg cacgcttcat cggcgttttc tgaccaaact
     3661 gcgggatctt atcctgcgtt tgtccgccgg aaatttgcag tgccgtccag atagggcctg
     3721 gcgcgacaat attcacccga atacctttct ccgcgacctg ttttgccagg ccacggctgt
     3781 agttcagaat cgccgccttc gtagccgcat agtccagtaa atgcggactt ggctggtatg
     3841 cctggattga cgaagtggtg atgatacttg cacctttcgg tagcaggggg atcgcttcct
     3901 gggttagcca gaacagcgcg aaaacgttaa tggcaaaggt cttttgaaac tgttcgctgg
     3961 tgaggtctgc aatatccgga atggcaacct gtttcccggc gaccagcgcc ataatatcca
     4021 gcccgcctaa cgccttgtgc gcttcgtgaa ccagcgaacg ggcgaatttc tcatcgctta
     4081 aatcgcctgg cagcagaacg gctttgcgtc cgcattcttc aatgatcttt ttcacatcct
     4141 gagcgtcttc ttcttccacg ggaagataac tgatcgccac gtcagcccct tctcgcgcgt
     4201 aagcgatggc ggcagcgcga cctattccgg aatcgccccc tgtcaccagt gctttacgat
     4261 ctttcaggcg agcgctacca acataggttt tctcgccgca atccggtatc ggtgtcatct
     4321 tcgcctggat gcctggcgtc ggttgtttct gtttgggata ttcaccagtg taatactgcg
     4381 tggtcgggtc attttaaatg agacatcgtt tttctccctt caggttcaac gtcctttaag
     4441 ggtagacgct ctcgatgcgt tgataaggga accaggaaga tccctaaacc tcagaattat
     4501 gcgacaaagg tttaacgtat atgttgattt gctgttgcgt gctgtttact caattgcgat
     4561 atactgttgc ccgttttaac tacacgacag gaatgtatgg aacgtttttt tgaaaatgca
     4621 atgtatgctt ctcgctggct gcttgccccc gtgtactttg gcctttcgct ggcgttagtt
     4681 gccctggcgc tgaagttctt ccaggagatt attcacgtac tgccgaatat cttctcgatg
     4741 gcggaatcag atttgatcct cgtgttgctg tcgctggtgg atatgacgct ggttggcggt
     4801 ttgctggtga tggtgatgtt ttccggttat gagaatttcg tctcacagct ggatatctcc
     4861 gagaacaaag agaagctgaa ctggctgggg aaaatggacg caacgtcgct gaaaaacaaa
     4921 gtagcagcgt cgattgtggc aatttcttcc attcacttac tgcgcgtctt tatggatgcg
     4981 aaaaatgtcc cggataacaa actgatgtgg tacgtcatta tccatctgac gtttgtgctt
     5041 tctgcatttg tgatgggcta tcttgaccga ctgactcgtc ataatcactg atctgattcg
     5101 ggcgcggttc gcgcgcccgt tattaacagg tcatttatcg gacgacgcct gccacagatt
     5161 cagttcgcca tcggcgatat gctgatcaat ctgcgccagc tcctcggtgc taaatgtcag
     5221 attattcagc gcctgcacgt tctcctctag ttgctccgcg cggctggcac caatcaatac
     5281 cgacgtcacg cgatcatctt tcagcaacca gcttaacgcc atttgcgcca ttgattgtcc
     5341 acgctgctgt gccatttcat tcaataagcg caggctgttg aggttggctt cggtaagcat
     5401 tttcggcgtc agaccacgaa ttttattccc ttcacgatgc atccgtgaat cttgcggaat
     5461 gccgttgaga tattttccgg tcagcaatcc ctgagccaga ggagtaaagg caatacagcc
     5521 cacgccgtta ttttgcaggg tatccagcag gccgctttta tccacccagc ggttcagtaa
     5581 attgtacgaa ggttgatgaa ttaaaagcgg aattttccac tcgtgcagca actcgaccat
     5641 tttttgcgtc cgctctggcg agtaagagga gatcccgaca tacagcgcct taccgctttg
     5701 taccgcatga gccagcgcag aggcggtttc ttccatcggc gtattttcat cgacgcgatg
     5761 agagtaaaag atatcgacat actcaagccc catacgcttc aggctttggt cgaggctggc
     5821 gagcaggtat ttacgtgaac cgccagagcc gtacgggccg ggccacatat cgtagccagc
     5881 cttggtagag ataatcagtt catcgcgata agcagcaaaa tcctcgcgca gcaggcgacc
     5941 aaagttctct tctgcgcttc ctggaggcgg cccgtaattg ttggctaaat caaagtgcgt
     6001 aatgcctaaa tcaaacgctt tacgcaggat tgcacgctgt gattccagcg cgttaacgtg
     6061 accgaaattg tgccataaac cgagcgataa cgcgggcagg cgtaaaccac tttttccgca
     6121 atagcggtac tgcatctgcc cgtaacgttc gggattcgct aaccagacca tgacctctcc
     6181 tttccaccgt tcaatttcga aacaatgttt ctagtttagc gattcgccag cgcgtatccc
     6241 gtagtctggc tcacagagtg acgaaaaact gggcaaaaac acgcgcttat gctttgctta
     6301 aaaaaacacc agttgaggaa tgcaacgatg ccgcgtttaa ccgccaaaga tttcccacaa
     6361 gagttgttgg attactacga ctattacgtt cacgggaaaa tttcgaaacg tgagttcctc
     6421 aaccttgcgg cgaagtatgc ggtgggcggg atgacggcat tagcgttgtt tgatttgctc
     6481 aagccaaatt atgcgctggc gactcaggta gagtttaccg acccggagat tgttgctgag
     6541 tacatcacgt atccttcgcc aaatggtcac ggcgaggtac ggggttatct ggtgaaaccc
     6601 gcaaaaatga gcggcaaaac gccagccgtg ggggtggtgc atgagaatcg tggactgaat
     6661 ccgtatatcg aagatgtggc acggcgagtg gcgaaggcgg ggtatatcgc cctggcacct
     6721 gacggattaa gttccgttgg aggttatccg ggaaatgatg ataaaggtcg tgagctgcaa
     6781 cagcaggttg atccaaccaa actgatgaat gatttctttg ccgcaattga gtttatgcaa
     6841 cgctatccgc aagcgacagg taaagtgggt attaccggat tttgctatgg cggtggcgta
     6901 tcgaacgcgg cggctgtcgc gtatccggaa ctggcctgcg cggtgccgtt ttatggtcgt
     6961 caggcaccca ctgccgatgt ggcgaagatt gaagcgcctt tactactcca cttcgcggaa
     7021 ctggacaccc gaatcaacga gggctggcct ccttacgagg cggcgttgaa agacaataat
     7081 aaggtttatg aggcttatat ctatccgggg gttaatcacg gattccataa tgattccacg
     7141 ccccgttatg acaaatctgc cgccgatctt gcctggcaaa ggacactgaa atggttcgat
     7201 aaatatctct cctgataggt ttatctctta cgggattacg tcttaaacaa gtatgaaaaa
     7261 atagcgtgcg caaaagtcgt tctttgccta aaatatcgct atatatggcg atatataacg
     7321 aatggggtga acgatgaata accattttgg taaaggctta atggcgggat taaaagcaac
     7381 gcatgccgac agtgcggtta atgtgacaaa attctgtgcc gattataaac gcggttttgt
     7441 attaggctac tcacaccgga tgtacgaaaa gaccggagat cgccagctta gcgcctggga
     7501 agcgggtatt ctgacgcgcc gctatggact ggataaagag atggtaatgg atttctttcg
     7561 tgagaataat tcctgttcta cgttgcgctt ttttatggcc ggttatcgcc tcgaaaattg
     7621 atcaaacata cgtattatct tgctttaatt aattacacta atgcttcttc ccttcgtttt
     7681 agcgccccgc cgcagtatca tgatatcgat aaccataata aatgtgtggt aaatggcgca
     7741 tcgatcgcat tattgatttt gcgattgagg caaaatatat gccaggtcct cgcaacggaa
     7801 taactataaa tgactggaga taacaccctc atccattctc acggcattaa ccgtcgtgat
     7861 ttcatgaagc tttgtgcagc attagccgcc accatggggt taagtagcaa agccgctgca
     7921 gagatggccg aatcggttac taacccgcag cgtccgccag ttatctggat tggcgcgcag
     7981 gagtgcaccg gttgtacgga atctctgctt cgtgcaacgc atccaacggt agaaaacctc
     8041 gtgctggaga ctatctctct ggaatatcac gaagtgcttt ccgccgcctt cggtcatcag
     8101 gtcgaagaga acaaacataa cgctctcgag aagtacaaag ggcagtatgt gttagtggtg
     8161 gatggttcca tcccattaaa agataacggt atttattgca tggttgccgg tgagccgatt
     8221 gtggatcaca tccgcaaagc ggcggaaggc gcagcagcca ttatcgctat tggttcctgc
     8281 tccgcgtggg gcggtgttgc cgcagctgga gttaacccaa ctggcgcagt cagcttgcaa
     8341 gaagttctgc caggcaaaac cgttatcaat attccgggct gcccgccgaa cccgcacaac
     8401 ttcctcgcga ccgttgcgca catcatcact tacggcaaac cgccgaaact ggatgacaaa
     8461 aaccgtccga ccttcgccta tggccgtctg attcacgaac actgcgaacg tcgcccgcac
     8521 ttcgatgctg gtcgttttgc caaagagttc ggtgatgaag gccaccgcga aggctggtgc
     8581 ctgtaccacc tcggctgtaa agggccagaa acttacggca actgctcaac gctgcaattc
     8641 tgcgatgttg gcggtgtgtg gccggtggcg attggtcacc cgtgctatgg ctgtaacgaa
     8701 aaaggtatcg gcttccataa aggcatccat cagcttgcca acgtcgaaaa tcagactccg
     8761 cgttcacaga aaccggatgt taacgctaaa gagggcggca acgtctctgc aggcgctatt
     8821 ggtttgctcg gcggtgtggt tgggctggtt gccggtgtca gcgtgatggc ggtgcgtgaa
     8881 ctgggtcgtc agcaaaagaa agataacgct gactcacggg gagaataacc gtgaacagac
     8941 gtaattttat taaagcagcc tcctgcgggg cattgctgac gggcgcgctg ccgtctgtca
     9001 gtcatgcggc tgctgaaaac cgcccgccaa ttccgggatc gctggggatg ttgtacgact
     9061 cgaccttgtg cgtaggctgc caggcttgcg tcaccaagtg tcaggatatc aacttccctg
     9121 aacgtaaccc gcaaggggaa cagacctggt cgaacaacga caaactgtcg ccgtatacca
     9181 ataacatcat tcaggtgtgg accagcggca caggggtcaa caaagaccag gaggagaacg
     9241 gctacgcgta cattaagaaa cagtgtatgc actgcgtcga tccgaactgt gtctctgtgt
     9301 gcccggtctc tgcactgaaa aaagatccga aaaccggcat tgtccattac gacaaagacg
     9361 tgtgcaccgg ttgccgttac tgcatggtcg cctgtccgta caacgtgccg aagtacgact
     9421 acaacaaccc gtttggtgcg ctgcataagt gcgagctgtg caaccagaaa ggtgtggaac
     9481 gtctcgataa aggcggtctg cctggctgcg tagaagtgtg cccggcgggc gcggtgattt
     9541 tcggtacgcg tgaagagctg atggcggagg cgaaaaaacg tctggcgctg aagcctggca
     9601 gcgaatacca ctatccgcgt cagacgctga aatctggcga cacttacctg catacggtgc
     9661 cgaaatatta tccgcatctg tacggcgaga aagagggcgg cggtactcag gttctggtac
     9721 tgacgggtgt gccttatgaa aatctcgacc tgccgaaact ggacgatctt tctaccggtg
     9781 cgcgttccga aaatattcaa cacaccctgt ataaaggcat gatgctacca ctggctgtgc
     9841 tggcgggctt aaccgtgctg gttcgtcgca acaccaaaaa cgaccatcac gacggaggag
     9901 acgatcatga gtcatgatcc acaaccgctg ggtggcaaaa tcatcagtaa accggtcatg
     9961 atttttggac cgttaatcgt catctgtatg ctcctgattg tgaagcgtct ggtgttcggt
    10021 ctgggctctg tctctgacct gaacggcggc ttcccgtggg gcgtgtggat cgcgtttgac
    10081 ctgctgattg gcaccggctt tgcctgtggc ggctgggcgc tggcgtgggc ggtatacgtc
    10141 tttaaccgtg ggcaatacca tccgctggtg cgtccggcgc tgttggcgag tctgtttggt
    10201 tactcactgg gtggcttgtc gatcactatc gacgtgggtc gctactggaa cctgccgtac
    10261 ttctacattc cgggtcactt caacgtgaac tcggtactgt tcgagacggc ggtctgtatg
    10321 accatctaca tcggcgtgat ggctctggag tttgctccgg cactgtttga acgtctgggc
    10381 tggaaggtgt cgctacaacg tctgaacaag gtgatgttct tcatcatcgc gctcggtgcg
    10441 ctgctgccga ccatgcacca gtcttcaatg gggtcgctga tgatctcggc gggctacaag
    10501 gtgcatccgc tctggcagag ctatgaaatg ttgccgctgt tctcgctgct gacggcgttc
    10561 atcatgggct tctcgattgt catctttgaa ggttcactgg tgcaggcggg tctgcgtggc
    10621 aacggtccgg atgaaaagag cctgttcgtc aagctgacca acaccatcag tgtgttgctg
    10681 gcgattttca tcgggctgcg ctttggcgag cttatctatc gcgacaagct gtcgttagcg
    10741 tttgccggtg acttctactc cgtgatgttc tggattgaag tcctgctgat gctcttcccg
    10801 ctggtcgttc tgcgtgtggc gaagctgcgt aatgattccc gcatgctgtt cctgtcagca
    10861 ctgagcgcac tgttaggttg tgcaacctgg cgtctgacct attcgttggt ggcattcaac
    10921 ccgggcggcg gttacgccta cttcccgacc tgggaagaac tgttgatttc tattggtttt
    10981 gtggctattg agatttgcgc ttacatcgta ctcattcgtc tactgccgat acttcctcct
    11041 ttaaaacaaa acgatcataa tcgtcatgag gcgagcaaag catgagccag agaattacta
    11101 ttgatccggt aacccgtatt gaggggcatt tacgcatcga ttgcgaaatc gaaaatggcg
    11161 tcgtttcgaa agcatgggct tccggtacca tgtggcgcgg catggaagag atcgtgaaaa
    11221 accgcgatcc gcgcgatgca tggatgattg tgcaacgtat ctgtggcgta tgtactacca
    11281 ctcacgcgct gtcttccgtt cgtgcggcag aaagtgcgct gaatatcgac gttccggtta
    11341 acgcgcaata catccgtaac atcattctgg ctgcgcacac cacgcatgac catatcgttc
    11401 atttctatca gctttcggcg ctggactggg tggacatcac ttctgcactg caagctgacc
    11461 caaccaaagc ctccgaaatg ctgaaaggcg tttcgacctg gcatctgaac agtccggaag
    11521 agttcactaa agttcagaac aagatcaaag atctggttgc cagcggtcag ttgggtattt
    11581 tcgccaatgg ctactggggt cacccggcga tgaaactgcc gccggaagtg aacctgattg
    11641 cggtagcgca ctacctgcag gcgctggagt gccagcgtga cgctaaccgc gtcgtggcgc
    11701 tgctgggcgg taaaacgccg cacattcaga acctggcagt gagtggtgtc gcgaacccaa
    11761 tcaacctcga cgggttgggc gtgttgaacc ttgagcgcct gatgtacatc aagtctttca
    11821 tcgacaaact gagcgacttt gttgagcagg tttataaggt tgataccgcg gttatcgccg
    11881 cgttctaccc ggaatggctg acgcgcggta aaggtgcggt gaactacctg agcgtgccgg
    11941 aattcccgac cgacagcaaa aacggcagct tcctgttccc gggcggctac attgagaatg
    12001 cggatctgtc ctcgtatcgt ccgatcactt ctcattccga tgaatacctg atcaaaggca
    12061 ttcaggaaag cgcgaagcac tcctggtata aagacgaagc gccacaggcg ccgtgggaag
    12121 gtaccaccat tccggcttat gatggttggt ctgacgacgg gaaatattcc tgggtgaaat
    12181 caccgacttt ctacggcaaa acggtagaag tgggtccgct ggctaatatg ctggtgaaac
    12241 tggcggcagg tcgcgaatct acccagaaca aactgaatga aatcgttgcg atttatcaga
    12301 aactgactgg caacacgctg gaagtggcac agctgcactc cacgctgggc cgtattattg
    12361 gtcgtaccgt tcactgctgt gaattgcagg atatcctgca aaaccaatac agtgcactga
    12421 tcaccaatat cggcaaaggc gatcacacca cctttgtgaa gccgaacatt ccggcaacgg
    12481 gtgagttcaa aggtgttggc ttcctcgaag cgccgcgcgg tatgctctct cactggatgg
    12541 ttattaaaga cggtatcatc agcaactacc aggcggttgt tccatcaacc tggaactctg
    12601 gtccgcgtaa cttcaatgat gacgtcggtc cttacgagca gtcgctggtg ggtacaccgg
    12661 ttgccgatcc gaataaaccg ctggaagtgg tgcgtaccat tcactccttc gacccgtgca
    12721 tggcctgtgc ggtacacgta gtggatgccg acggcaacga agtggtttca gtgaaggttc
    12781 tgtaatgcgt attttagtct taggggtcgg caatattttg ctgaccgatg aagccatcgg
    12841 cgtgcggatt gtcgaagcgt tagagcaacg atacattctg ccggattatg ttgagatcct
    12901 cgatggcggc acggcgggaa tggagctgct tagcgacatg gcaaatcgcg atcatctgat
    12961 tattgcggat gccattgtgt cgaaaaagaa cacgccggga acgatgatga tcctgcggga
    13021 tgaagaagtt ccggcgttgt ttaccaacaa aatctctccg catcagcttg gcctggccga
    13081 cgtattgtcg gccctgcgct tcaccggtga gtttccgaaa aagctgaccc tggtcggcgt
    13141 gatcccggaa tcgctggagc cacacatcgg cttaacgccg acggttgaag caatgattga
    13201 acctgcgctt gagctggttc tggctgcgct gcgcgaatca ggcgtggaag ccatcccacg
    13261 ggaggcgatt catgactgaa gagatagcag gtttccagac ctccccgaag gcgcaagtac
    13321 aggcagcgtt tgaagaaatt gcccggcgtt cgatgcacga tctctctttt ctgcatcctt
    13381 caatgccggt gtatgtttct gattttacgc tgttcgaagg tcagtggacg gggtgtgtga
    13441 tcaccccgtg gatgctgagt gcagttatct tccccggccc ggatcaactc tggccgctgc
    13501 gcaaagtgag tgaaaaaatt ggtctgcaac tgccgtatgg caccatgacc tttaccgttg
    13561 gcgaactgga cggtgtttcg caatacctct cctgttcgct gatgtcgccg ctttcgcaca
    13621 gcatgtcgat tgaagaaggc caacgcctga cggatgactg cgcacgaatg atcctttcgt
    13681 tgccagtcac caatccggat gtaccacacg cagggcgtcg cgccctgctg tttggccgca
    13741 ggaggggcga aaatgcatga gttgtcgctt tgccagagcg ccgttgaaat tatccaacgg
    13801 caggcggagc agcacgatgt taagcgcgtc accgccgtgt ggctggaaat tggcgcgctc
    13861 tcctgcgttg aggagagcgc cgtccgtttt agttttgaaa ttgtctgcca cggaacggtg
    13921 gcgcaagggt gcgatttaca tatcgtctat aaacccgccc aggcctggtg ctgggattgc
    13981 agccaggtgg tggagattca tcagcacgat gcgcagtgtc cgctctgtca cggcgagcgg
    14041 ttgcgtgtcg ataccggcga ttcgctgatc gtcaaaagta ttgaagttga ataagactga
    14101 ggacaacgcc taaaaagcgc acggacgggc ccctcgcccc tccggggaga gggttagggt
    14161 gagagggaac acccgctaaa ggttcttttt cgtgtttgtc cggcaatcga aataaccgga
    14221 gttaacaatg tgtattggcg ttccaggcca ggtgctggct gtcggtgaag atattcacca
    14281 gcttgcgcag gttgaagtat gtggtatcaa gcgcgacgtg aatatcgccc tgatttgcga
    14341 aggtaaccct gccgatctgc tgggccagtg ggtgctggta cacgtcggat ttgccatgag
    14401 catcatcgac gaagatgaag ccaaagccac attagacgca ctgcgccaaa tggattacga
    14461 cattaccagc gcgtgatgat tagcttttgt aggcctgata agacgcggca gcgtcgcatc
    14521 aggcattgtg cacgattgcc ggatgcggcg tgaacgcctt atccggcctg gatggcttgc
    14581 tgcgacgaac accaaccctt acccctgacg cttatcttct gtatttgtct caaaatcact
    14641 ggcgtcatgg cgctcatgca actgctcgtt cagcggcccg ttggtgcggt taacaatacg
    14701 cccacgtttc actgccggac gttcgcccac ttctttcgcc cagcgttgca catgtttata
    14761 acttcccgca tccagaaact ctgcggcatc atacacgccg ccgagcacca cgttgccaaa
    14821 ccacggccaa atcgccatat ccgcaatggt gtactcatcg cccgcaacaa acttatgctg
    14881 cgccagttgc ttatccagca cgtcgagcag gcgcttggct tccatggtaa agcggttgat
    14941 ggcgtactca attttcaccg gcgcgtaatg gtaaaagtga ccaaaaccac cgccgaggaa
    15001 cggcgcggca ccctgtaacc agaacagcca gttcaacgtt tcagtccgtt ttgccagatc
    15061 ctgcggcagg aagtagccaa atttctccgc cagataaagc aggatcgaac cagattcaaa
    15121 cacgcggatc ggcggattat gcgtatgatc gcgcagcgcc gggatcttcg agtttgggtt
    15181 cacttcgaca aagccgctgg agaattgatc gccatcgcca atacgaatca gccaggcgtc
    15241 gtactctgca ccagtaacgc ccagcgccag tagctcctca agcataatcg ttactttctg
    15301 accgttcggc gttcccagcg aataaagttg caatggatgt ttgccaacgg gcagcgtttt
    15361 ttcatgcgtc ggaccagaaa acgggcgatt gatattggcg aacgcgccgc cagcggattt
    15421 atcccacgtc cagactttcg cgggctgata agtattgtct gtcatagtga gttgccttct
    15481 gagtggtagt gttgaagcag tgtagcaggt cacttgctgt acagaatgtg aattttttaa
    15541 ccctttgcgc cagccgctgc gatgaggtgt atgattagtc tgacctctgt ccggttaatg
    15601 tcgctgagca ggtaatcaga gcacaacaat tacgccaact gcttaggttg tattgttaaa
    15661 ggtaaagtga tgagcaaagg aacgaccagc caggatgccc cgttcgggac attattgggc
    15721 tacgccccag gcggggtagc aatctactct tcagattaca gttctctcga tccgcaggaa
    15781 tacgaagatg acgccgtatt ccgtagctat atcgacgacg aatatatggg ccacaagtgg
    15841 caatgcgttg aatttgctcg ccgttttctc tttctgaatt acggtgtggt ctttactgac
    15901 gtgggtatgg cgtgggagat tttctcgctg cgcttcctgc gagaagtggt taatgacaac
    15961 atcctgccat tgcaggcatt tcctaacggc tcgccgcgtg cgccggtcgc gggtgcgctt
    16021 cttatctggg ataaaggcgg tgaatttaaa gacactggcc atgtcgccat cattacccaa
    16081 ttgcatggca acaaagtccg tattgcggaa cagaacgtga ttcattcccc gttgccgcaa
    16141 gggcaacagt ggacgcgcga gctggagatg gtggtcgaaa acggctgcta tacccttaaa
    16201 gacacttttg atgacaccac cattctgggc tggatgatcc agacggaaga tactgaatac
    16261 agcttaccgc agccggaaat tgcaggcgag ctgctgaaaa tcagtggagc gcgtctggaa
    16321 aacaaaggcc agtttgacgg taaatggctg gatgaaaaag atccgctaca aaacgcctat
    16381 gtgcaggcca acggtcaggt gatcaatcag gatccgtatc attactacac cattaccgag
    16441 agtgccgagc aggagctaat taaagccacc aacgagctgc atctgatgta tcttcacgca
    16501 accgacaagg tgctgaaaga tgacaacctg ctggcgctgt tcgacattcc gaaaatcctc
    16561 tggccacgtt tgcgtctctc ctggcagcgt cgccgtcacc atatgatcac tggtcgtatg
    16621 gatttctgca tggatgagcg tggcctgaag gtttacgaat acaacgccga ctccgcctcc
    16681 tgtcataccg aagcgggctt gatcctcgaa cgttgggcgg agcagggcta taaaggcaac
    16741 ggcttcaatc cggcggaagg gctgattaac gaactggctg gtgcctggaa acacagtcgt
    16801 gcacgtccgt ttgtccatat catgcaggac aaagatatcg aggaaaacta tcacgcgcag
    16861 tttatggagc aggcgctgca ccaggcgggc tttgaaacgc gtatcttgcg cgggctggat
    16921 gaactgggct gggatgctgc cgggcaactg attgatgggg aagggcgact ggttaactgc
    16981 gtgtggaaaa cctgggcgtg ggaaaccgcg tttgatcaga ttcgtgaagt tagcgaccgt
    17041 gagtttgctg cggtgccaat ccgtaccggt catccgcaaa acgaagtgcg tcttatcgac
    17101 gtattgctgc gcccggaagt gctggtcttt gagccgctgt ggacggtgat ccccggcaac
    17161 aaagcgattc tgccgatcct ctggtcgctg ttcccgcacc atcgttacct gctggatacc
    17221 gatttcactg ttaatgatga attggtgaaa acaggttacg cagtgaaacc gatcgccggt
    17281 cgctgtggta gcaatatcga cctggtcggc catcatgaag aggtgctgga caaaaccagc
    17341 ggtaaatttg ccgagcagaa aaacatctat cagcaactgt ggtgtttgcc gaaagtggac
    17401 ggtaaataca ttcaggtatg taccttcacc gttggcggca actacggtgg gacgtgtttg
    17461 cgcggtgatg aatcactggt catcaaaaaa gagagtgata ttgaaccgtt aattgtggtg
    17521 aaaaagtaat acatgttgta gcaaccccgt actgattatg aagaataatt agtacgggga
    17581 ttaatcaaaa tattcaatat ttatatattc aaatatttat tcatttcata tgtgaatata
    17641 ttaaccagtg gaatacctgt gttttgatta tttctaaagg ttttgaatga atgcttattg
    17701 tctaatacac aagaaataac actcttttta tcgttaaaaa atgatatttc actttgccca
    17761 tgccgttaaa gtcactgata atgcgtccgt tcgtaaattc aaaatggcgt aatctaatat
    17821 atgctaaatt tatttgttgg ccttgatata tatacggggc ttttgttatt acttgctctg
    17881 gcatttgtgt tgttctacga agcaatcaat ggttttcatg acacggcgaa tgcggtggca
    17941 accgttattt atactcgtgc catgcagcca caacttgctg tggtgatggc ggcatttttt
    18001 aacttttttg gcgtgttatt gggcggactt agcgttgcct atgccattgt ccatatgtta
    18061 ccaaccgatt tgttgctgaa tatggggtca actcacggtc tggcgatggt cttttccatg
    18121 ctgctggcgg cgattatctg gaacctggga acgtggttct ttggtttacc ggcctccagt
    18181 tcgcacacct tgattggtgc gattatcggc atcggtttaa ccaacgcgct gttaaccggc
    18241 tcatcggtga tggatgcgtt aaacctgcgt gaagtaacca aaattttctc ctcgctgatt
    18301 gtttccccta tcgtcggcct ggtcattgcg ggtggtctga ttttcctgct gcgccgtttc
    18361 tggagtggga cgaaaaagcg tgaccgtatt caccgcattc cggaagatcg caaaaagaaa
    18421 aagggcaaac gtaaaccgcc attctggacg cgtattgcgc tgattgtttc cgctgcaggc
    18481 gtggcgtttt cgcacggcgc gaacgacggg cagaaaggga tcggcctggt aatgctggta
    18541 ctggtgggga ttgcccctgc tggcttcgtc gtcaatatga atgcgtccgg ctatgaaatt
    18601 acccgtaccc gcgatgccgt taccaacttc gaacactacc tgcaacagca tcctgaactg
    18661 ccgcagaagt tgattgccat ggaacctcca ttgcctgcag catcgactga tggcacgcaa
    18721 gtaacagagt ttcactgtca tccggcaaat acctttgatg cgattgcgcg cgttaaaacg
    18781 atgctgccag gcaatatgga aagttacgag ccgttaagcg tgagtcagcg cagccagctg
    18841 cgccgcatta tgctgtgcat ctctgatacc tccgcgaagc tggcgaaact gccaggcgtc
    18901 agtaaagaag accagaacct gctgaagaaa ctgcgcagcg atatgttaag caccattgag
    18961 tacgctccgg tgtggatcat catggcggta gcactggcgc tcggcattgg caccatgatt
    19021 ggctggcgtc gtgtagcgat gaccatcggt gagaagattg gtaagcgcgg catgacgtat
    19081 gcgcaaggca tggcggcaca aatgacggcg gcagtgtcta tcggccttgc cagttatatt
    19141 gggatgcctg tctccacaac acacgtcctc tcgtctgcgg tagcagggac gatggtggtg
    19201 gacggcggtg ggttacagcg taaaacggta accagcatcc tgatggcgtg ggtgtttact
    19261 ttaccggcgg caatttttct ttctggtggg ctgtactgga tagcattgca gttgatttaa
    19321 ccttcctgaa aatgcccggt cctggcgatc gggcatttcc atttttgact agtgataacc
    19381 acgcgcggtc ataaaatccg taatcgcttt ttctgcatca accaacacct gttccagagg
    19441 ctgattggca tcgatatcaa ccagttgtgc accaccaaag gttaactgtg gcgttatggc
    19501 aatcttcctc gccagcgatt cccgtttatg gtcgggttta cgtgcacagg caacttcaag
    19561 gtcaacattg agtttgatga ccagatcagg cttatggctc gccatccagt gaaacgcttt
    19621 acgttcctgg cttgccagcc atgagacaaa acgaccacct tcaacgttag gtgggaacac
    19681 cgtaccatcg taagcgccag gaatttggtc ctgaggataa cggtcggtta gaacaattaa
    19741 cccgcgacga cgacaggcaa gcatatgacg aaagcgcagt aagcgacggg cgacaaacgc
    19801 tgtaattacc agcgccggaa ctggtccagg caattttttt gctgttttca cctgatttcg
    19861 ttcaattgtt ttatgtaagg attttcccat caaaggtaat tttgtcactg cacgaccgac
    19921 atttccggcc tgttttccta aatgaactct ttcggcagca ccatattttt cgacaacggt
    19981 aataagatgt tcacacaccg ttgacttgcc tgaaccatcg ctaccaataa cggcaattaa
    20041 tggaggtgtt attgactgca tgaatatatc ctttaattta aatatccatt aaaaatattt
    20101 atttggttaa tatgttttta tgaaagcgta attcaggtca atgtcacaat taaccattgt
    20161 cacaataagg ttgaacggat attctgagca cggaccacgt aatattcaat acattattta
    20221 ctgccgggtt tttcgtgaat caacgtaata tgtcaataat taattccacg ccagtgcgtg
    20281 ttattgccat tgtaggatgt gatggttcag gtaaatcgac cctcacggca agcctggtaa
    20341 atgaactggc agcaagaatg ccaacagaac acatttatct cgggcaatcg tccgggcgaa
    20401 ttggcgaatg gatttcacag ctccctgtta ttggcgcacc ttgtgggtgt tatctgcgaa
    20461 gtaaagcggc acatgtacac gaaaagccct caacaccgcc tggcaatatt actgcgctgg
    20521 ttatctatct gctttcctgc tggcgggcgt acaagtttcg caaaatgttg tgtaaaagcc
    20581 agcaaggcta tctgctcatc accgaccgct acccgcaggt tgaagtgccg ggttttcgct
    20641 ttgatggccc gcaattggca aaaaccacgg gcggtaacgg ttggataaaa atgttaaggc
    20701 agcgcgagtt gaagctgtac caatggatgg cgtcttattt acccgtattg ttgattcgtc
    20761 ttggcattga tgaacaaacc gcgtttgcgc gtaaacctga ccaccaactg gcagcgttac
    20821 aggaaaaaat cgccgttacg ccgcaactga cattcaatgg cgccaagatc cttgaactgg
    20881 atggtcggca accagccgat gaaattatgc aagcgtcact acgcgcaatt cacgccgcgc
    20941 tttcctgatc accaaaatgg ataggggatg atttaacgaa tggatgcact acaaacacaa
    21001 actgttaata gtacaaccgc gccgcagccc aactacattc cggggctgat tgcggtggtc
    21061 gggtgtgatg gcaccggtaa atccacgctg accaccgacc tggtgaaatc gctgcaacaa
    21121 cactggcaaa ccgagcgccg ctatctgggg ctgctctccg gcgaagacgg agacaaaatc
    21181 aaacgattgc cgttggttgg cgtctggctg gaacggcgac tggcggccaa atcctcgaaa
    21241 acccaaagca tgaaaaccaa atctccggcg ctatgggcgg cggtgattat gtactgcttc
    21301 tcgctgcgaa gaatggcgaa tctacgcaag gttcagcgac tggcgcaaag tggcgttctg
    21361 gtggtcagcg atcgcttccc gcaggctgaa atttcgggct tttattatga cggaccgggg
    21421 atcggcgtcg aacgtgcgac cgggaaaatc agcatgtttc tggcgcagcg cgaacggagt
    21481 ttataccaaa aaatggcgca ataccgtcct gaattaatca ttcgtttggg gattgatatt
    21541 gatactgcca tctcccgcaa gcctgaccat gagtatgccg agctgcagga caaaatcggc
    21601 gtcatgtcga cgattggcta taacggcaca aaaattcttg agattgattc cagagcgccc
    21661 tacagcgaag tgctggaaca ggtacagaag gcggtttctc tggttgccat cgtttctgac
    21721 cgccgaagtt taacttaagg ctggcgggat attttttact atcaacgata aggtcaggct
    21781 gaattggctg gttttaacat caaacattgg tttgcagatg gcgcgtttcg caccattatt
    21841 cgcaatagcg cctggttagg ctccagtaat gtcgagagcg ccttgctggg gctgttggcg
    21901 ctctcgtgtg ccggtaaagg gatgacgccc gccatgtttg gtgtactggt gattgtgcaa
    21961 tcgtacgcca agtcgatcag cgattttatt aagtttcaga catggcagct ggtggttcag
    22021 tacggaacgc cagccttgac caacaataat ccgcagcaat ttcgcaatgt cgtctcattt
    22081 tccttctcgc tggatatcgt cagcggcgcg gtggcgattg tcggcggcat tgccttactg
    22141 ccattccttt cccattcatt aggtctggat gaccaaagct tttggctggc ggcgctgtat
    22201 tgcacgctca ttccttcaat ggcttcctcc acgccgaccg gcattctgcg tgcggtagat
    22261 cgctttgatt tgattgctgt acagcaggcg acgaaacctt ttctgcgcgc agcggggagc
    22321 gtcgcagcct ggtattttga ctttggtttt gcgggttttg ttattgcctg gtacgtgtcg
    22381 aatctggttg gcggcaccat gtactggtgg tttgccgcgc gcgaattacg ccgtagaaat
    22441 atccataacg ccttcaaatt gaatctgttt gagtctgccc gacacattaa aggcgcgtgg
    22501 agttttgtct ggtcaaccaa cattgcccac tccatctggt cggcgcgtaa ctcgtgcagc
    22561 acagtgttgg tggggatcgt gttaggaccc gctgccgctg ggttatttaa aatcgccatg
    22621 acattcttcg acgccgccgg aacgccagcg ggtttgctgg gtaaaagttt ttacccagag
    22681 gtgatgcgtt tagatccgcg caccaccaga ccgtggttgt taggtgtgaa gtccggttta
    22741 ctggcgggcg gaatcggtat tctggtggcg ctcgcggtgt tgatcgtcgg caagccgctc
    22801 atttcgctgg tgtttggcgt taagtatctc gaagcgtatg acctgattca ggtgatgctg
    22861 ggcgcaattg tgatttcgat gctgggcttc ccgcaggagt cattgctatt gatggcggga
    22921 aaacagcgcg cttttctcgt ggcgcaaacc atcgcctcaa tcggctacat cgtattgctg
    22981 ttcatgttct gtcatctgtt tggtgtgctg ggagcagcgt ttgcttactt cggtgggcaa
    23041 tgtctggacg tggcgctctc gttaattcca accttaaagg cattttttca gcgccattcc
    23101 ttgctttata acgcagccgg agagaaatcc tgatgatgag aaaatatttc ccgcttgagg
    23161 cgagcgagcg tctatttgtt gcgattgaag aagatgatgt cgttgatgca caggttagtt
    23221 tgccgccgac tattgcacta agttgtacca ccgaaatcat tcatgataat tacgcgttat
    23281 gcctgcaatt ctggctcaat ggcgttgatc gtcaggaatt actgcgttta gttcgcaaac
    23341 aagccaaagg cgatgagctg actgctgatg agcgcaaaca atttaaatac atgcgagcgc
    23401 gctacaagca cctgcgtttt gcgcaacggc tgtatttaaa gaagcatcag gccggatttt
    23461 tattcggcaa aacgaccgtt tttctggggc gttttcagga cggctttcgt aacggtaaga
    23521 aaaatattgt gtcgtattac ggcaacctgt tacggattta tttaagctct ccggtctggt
    23581 cgttggtgaa ctattcatta cgccatagcc agttagaaag cgtaagcagt tttattgcct
    23641 atcggcaaaa gcaaatgcat accctgaaag agattattgc caaaccgcga ctaaccggca
    23701 gggaatttca tgatgtgcga aaaatcatca gccagcaggt ttcttattac gacaccttaa
    23761 gatcgcttga tcctgaaaat aaagaggcgt tacagatttc ccgtttcctc gccgccatta
    23821 atggcctgat gggagataaa cacgacgaca tggtggcgga tgatatggaa aaccgccagt
    23881 cgtattatgc accgttggcg ctggatagcg atatttgtca gcgcttagag ctgctgattt
    23941 cgcgttttcc gctatagccg gagcaatgat gtcatctcgt ttgattattg cgttgattat
    24001 catgctctta gccccgggtg tgcaggcaca caatttcgtc actggcaaaa cggttacccc
    24061 tgtttatatc caggaaggcg gcgagctgct tctgaatagc gacgacgaaa ttcactacca
    24121 aaagtggaac agcacacaat tagcgggaaa agtccgcatt attcagtata tcgccgggcg
    24181 taagtcggcg aagaaaaaga actcactgtt aattaaagct gttgaagcgg caaactttcc
    24241 gcaggatcgt tttcagccca ccaccattgt gaacaccgat gatgccatct ttggtactgg
    24301 ctattttgtt gttggcaaaa tcgaaaaaaa taaacgccgc tatccgtggg cgcaattcgt
    24361 tattgacggc aatggacagg gacgcgttgc ctggcgattg ccggagcaga gttcaaccat
    24421 tctggtgctg aataaagccg ggcaaattca gtgggcaaag gacggcagcc tgacgccgga
    24481 agaggtagat catgtcattg cgttggcgca gaaactgagc aatgaataaa aaactcaaag
    24541 gattgagggc gttagtgaga tgaagctggt tgaacactac atcatgcgtg gtacgcgccg
    24601 tctggtgctg attatcgtcg gttttctgat ctttattttc gccagctact ccgcacagcg
    24661 ttatctgacc gaagcggcaa atgggacatt agctcttgat gttgtgctgg atatcgtttt
    24721 ctacaaagta ctgattgcac tggagatgtt gttacctgtt ggtctgtatg tgtcagttgg
    24781 cgtgacgcta gggcagatgt ataccgactc ggaaattacc gctatctctg cggcgggggg
    24841 cagtccggga cgcttgtaca aagccgttct ttatctggcg ataccgctaa gtatttttgt
    24901 cacccttctg tcgatgtatg gtcgtccgtg ggcttatgcg cagatttatc aactggagca
    24961 gcagtcgcag tcggagctgg atgttcgcca gttgcgggca aagaaattta acactaacga
    25021 taacggacga atgatccttt cgcagacggt tgatcaggat aataatcgcc tgactgacgc
    25081 gctgatttat acttctactg ccaatcgaac ccgtattttc cgcgcccgtt cggttgatgt
    25141 ggttgaccca tcacccgaga aaccgaccgt tatgttgcac aacggcactg cctatcttct
    25201 cgatcatcag gggcgtgacg acaacgaaca gatctaccgt aatctgcaat tacatctgaa
    25261 tccgctggat caaagcccga acgtcaaacg caaagcgaaa tcggtcacgg agctggcgcg
    25321 ctccgccttt cctgccgatc atgccgaact gcaatggcga caaagccgtg gcctgaccgc
    25381 attgttgatg gcgctgctgg ccatttcatt aagtcgggta aaaccgtggc aagggcgatt
    25441 ttcaacgtta ttgccactga cgttgctgtt tattgccatt ttttatggcg gcgacgtctg
    25501 ccgtacactg gtggctaacg gcgcgattcc cctcattcct ggtttgtggt tagtgcccgg
    25561 actcatgcta atgggcctgc tgatactggt cgcacgtgac ttctctttgc tgcagaaatt
    25621 ttcccgatga atgttttcag tcgctattta atccgtcatc tctttctcgg ttttgccgcc
    25681 gctgcagggc tattgctgcc gctttttacc accttcaacc tgattaacga actggatggt
    25741 gtcagcccgg gcggttatcg ctggactcag gcggtgctgg tggtgctaat gaccttaccg
    25801 cgcacactgg tcgaactttc gccgtttatc gcgttattgg gtggtattgt tggactgggg
    25861 cagttatcga aaaacagtga gcttaccgcc attcgcagca cggggttttc tatcttccgt
    25921 attgcactgg tggcgctggt tgccgggata ttgtggactg tttcgttagg cgcgatagat
    25981 gagtgggtgg cgtcgccatt gcagcaacag gcgttgcaaa tcaaatcgac cgccaccgcg
    26041 ttgggggagg acgatgacat taccggcaat atgctgtggg ccaggcgtgg taatgaattt
    26101 gtgacagtga aatcgctgaa cgagcagggc cagccggtgg gcgtggagat atttcattat
    26161 cgcgacgatc tctcgcttga atcctacatt tatgcacgca gtgccaccat tgaagacgac
    26221 aaaacgtggg tcctgcatgg tgtgaatcat aaaaaatggc tgaatggcaa agaaacgctg
    26281 gaaacattag ataatcttgc ctggcaatcg gccttcacca gtatgaatct tgaagagtta
    26341 tcaatgccgg ggaatacttt ttctgtccgt cagcttaatc attacatcca ttatttgcag
    26401 gaaaccgggc aacccagcag cgaataccgc cttgctctgt gggaaaaact ggggcaaccc
    26461 atcctgaccc tggcgatgat tttgctggct gtgccgttta cctttagtgc cccgcgttcg
    26521 cctgggatgg gtagccgtct cgctgtaggc gtcatcgtgg gcttactcac ctggatcagc
    26581 tatcaaatca tggttaatct gggattgcta tttgcgttaa gcgcacctgt taccgcgctc
    26641 ggtttaccgg tagcgtttgt gttggtggcg ttgagcctgg tgtattggta tgacagacaa
    26701 cattaaaccc gccgtggcgc aaacaccacg gcgggctcac attactgctt cagagtagca
    26761 aatgcctgaa tgatggcatc gatttgctca cgactgtggg cagcatttac gctacagcgc
    26821 agcagcgtga tgcctgcagg cgcggcaggc ggcaggataa gattcacata cacgcccggc
    26881 gagataagct cgcgccagat gcgtaaacct tcctctttcg aaccgattat tactggcact
    26941 accgggctaa tatgtgaact taactcataa cccagttttt gcagaccgtc atacagatgg
    27001 ttggcgttat ccatcagctt ttgccgcagc tctggttgtg tggcaatagt tcttaacgac
    27061 gagcgcacgg tcgcaatgca ggacggagaa ggagaggcgg taaaaatata cgggcggctg
    27121 ccgtagcgca gcacttccat cgcttccgaa ccgacagcaa aaccgccaat cgaggccaga
    27181 cttttactga aagtaccaac gataatatcc acatcatctt caacgccgac agcttcagcc
    27241 agtccgcgcc ccgttgcgcc taacacgaca aacgaatgcg cttcatcgac aatcaaataa
    27301 ccaccgagac gacgtttgat atcaacaatt tccgccagcg gcgcaacgtc accgagcatg
    27361 ctgtagatgc cctcaacgat gataatcgcc tctttcgccc gttcaccgag gcgaaccatg
    27421 cgccgttcca gatctttagc atcgttatgg cggaaacgaa taatttccgc gccaccgaga
    27481 gaacaggcgt cataaatact ggcgtggctg tcggcatcca gtaacaccac ggcgttatgg
    27541 tcggcaagtg cgctgatgac gcctaaatta gcggtataac cggttgaaaa aacgatagcg
    27601 gtagggcgat tgaaaaactc ggcaatctct ttctctaacg ccaggtgcgg cgcataactg
    27661 ccgtttgcca tacgagaacc ggtggtgccg gtgccttggg ccgctaacgc ggcctgacct
    27721 tcgctgatgg catcatgatt aaaggttaaa ccgagataat tattggtccc ggccagaatc
    27781 acttttttat tgccgatccg gccttcggtg gcggaataca cttcatcaat acaggtgcca
    27841 aacggtgtta acccgttgtc actaaattgc agacgctcgc cagcaaggcg cgcatattta
    27901 tcgtatagcc ccattattta ttctccagcc atggaagtag agtttccatt aattgcgcag
    27961 ggggtttgac attcagcaga atattaatag gaatggaaat atcgaatctg tcttccagca
    28021 tcattaagag atccatcact ttgatcgact ccaggccaag atcgttgacg agatcgctgt
    28081 cgggcttaat ttccacaccg ttttcgacca aatcctgtaa acaggaaagg atgtaatcca
    28141 ttactatttc acgatttacc atagaaacgt taacacacct ttaaaacaag acaatcaaaa
    28201 tagcccgttg cgtaatgcgt gttccaggct aatcccggga aaccaattga tatcttcaga
    28261 aatacggtta ttacttgccg accagtcggc gtgggttaat tcacgaattt tcgagcgggt
    28321 cagcataggt tctttacctg ccaggcgact caacgcggta ctgatatccg caaggcctgt
    28381 gagtaccggc agaggaatgt caaccattcg cacggaacca caacgggctt tggcggcaag
    28441 ctgtcgtacg cgttgccagt catagccacc ggcgacgcca tcgcataatt cataggtttg
    28501 cgtctgtaca gtttcggcgc ttaaccactg acccactgct tgcgcaaaat cggtgacgtg
    28561 aagaaaagag agttgtgtat ctggcgcacc aagtcgtggc agcaggccgc gtagcatcca
    28621 gtcaaacagc ggttttaact ctttatcgcc gggaccatac acggctgtcg ggcgaaaaac
    28681 gcccagcgta atttcgtccg ccattgctgc cagtcgttgc tcggcgacgt atttggattt
    28741 tgcataccag gagagctcgg gatggcgcgc cgccagcgaa gagataaaca gaaaacgttg
    28801 gcaaaagccg ctctcttttg ctgcttgcat caggcgcagg ctgccgtcaa cgttacagcg
    28861 ggtgaaaatc tcttctttgt gcccgcgcac ttgcccggcg caatggacca ctacgctggc
    28921 tccggcaacc agctcgctaa gtgaatgagt atcttccagc gaaccgcgca cccaggtgag
    28981 attatcattg acgtgagcgc gggcagtacg cgtcaatgcg cgaacgtgaa agccgcgggc
    29041 gagcaggtta tcgataatat atttaccgat aaacccggta gcgcccgtca ccgcgacagt
    29101 ttgattcatg ccagggattc ctgcacatta agactggcag cataagcctt ctgataacgt
    29161 tttttcgctt ccgcacgggc aggcttgccg gaggacgttc ggggaatact gtggggcggc
    29221 aacagatcga tagccgcggt cacgccaaat tcgctttgga tccgtgccgc cagcgcgtgg
    29281 ataagctgcc cgcgacgttc ttcgtcgctg atccgacact ggatctgcaa aatgattttt
    29341 tcctgggcgg taacaaaagc aatcgcatcg ccagaatgaa tttccggttc ctgttccgct
    29401 atatattcaa tatcctgcgg ccagatatta cggccacgaa taataatcag atctttaatg
    29461 cgtccggtga catacagata accgtccagc agataaccga ggtcgccggt gtctaaccag
    29521 cccgtcgcgg caatctcgtc ttgcgaaacc tggtcgccaa agtaaccgct catcagactg
    29581 ggaccggaga tgcaaatatg gcctaccaca cgttccgcga ccggcatacc tgcttcattg
    29641 cggatttcaa taccatgttc cggcaacgct ttgccgcagt tgacgaaagt cgatacggcg
    29701 cgtgtctctg cacccggcgc gacggcttta ccctgatatt cgaggatgtc gcgatccact
    29761 tcgttaacca caaccccgga ggcttcatca gagaagctga cagccagcgc attttctgcc
    29821 agtccgtagc acggcatgaa agttttattg tcaaagttaa cctgacggaa acattcagcg
    29881 aattgatgga gttgttctgc ggagatcggc tcagcaccaa taccagcgac gcgccagtag
    29941 gaaagatcca gttcagcgag atctttttca ttcacgcggc gttggcacaa ttcatagcca
    30001 aacggcggcg caacggaaac ggtgccgcga tttttactga tcaatttaag ccattgcaaa
    30061 ggacgcatgg caaaatcctg agtgcgcaaa taatctactg aaagttgcgt ggcgacgggg
    30121 ggcaggagaa agccgaccag tcccatatca tggtagaaag gctgccagga gacgcagcgg
    30181 tcgctagggc gtaatttaat gccatcgtgg cttatagcac gtagattagc catcacttcg
    30241 cgatgggtga taatgacgcc acggggaaaa cgggtgctgc cggaggtgta ctggaggtag
    30301 gcgatatcgt tcggaactgg acgctggagc gcaacatcgg cttccggtaa tgccttaaac
    30361 caagcgtggc ttaaaacatg taattcgggg ttgttatgcg tcgcggcatt gaccagtggc
    30421 aactactcat caccagtgat aatggctgcg ggctggcaac ttgccagtaa accctagagt
    30481 ttggcgctcc aggaatcccg ctgaccgacg cccattggaa tcgccaacgg gaaggcgact
    30541 aagccagcat actgacaggc aaaaaaagcc tctacaaacc cgctactcgt ttcggcaatc
    30601 agcgccacgc gttcgccttt tttcaggttc agcgataaca accgcttcgc accagcttcg
    30661 gcacgcgttt ttaatgtctg atattccagt tgatcttcaa gttggcaacg tctgtcataa
    30721 aaattcattc cggctctact cagagcggcg tagtccaaag catcaaccag cgttggaaaa
    30781 tcggcatagc gcataggtag ggaatgcgta aagattttat tagacatata caccacgaaa
    30841 aaattggata tcaaaaataa aatgacaata atgcggtgga ttatataggc ataaaagata
    30901 aatgagatag tattttcatc ggtgtgaaaa tgaacattga tttaaataat gagtttgagt
    30961 tttttatttt tattatgaat gatatttaat ttatgaatat attaagaaga agccttgtcc
    31021 ggtcgcctta aatcgacaaa aggcattgag gcgtttctgt gtagcctctg accttatcat
    31081 aatcattaat taatactatt tattcgtcgt ttgtattcat tgagttgatt attaagatgc
    31141 cgctcgttgc tgttgcaacc tgtttttcac cattaattat ttgttatcca cagagatatg
    31201 gaatgcgatc tgctgatgat taaaattgaa aaagtaatta ataaaaatga ccttaaggca
    31261 tttatcgctt ttccgtcatc actttatccc gacgatccaa actggatacc ccctttattc
    31321 attgaacgca acgagcattt gtctgcgaaa aatccgggga cggaccatat catctggcag
    31381 gcgtgggtgg caaaaaaagc ggggcagata gttgggcgta ttaccgcgca aatagatacc
    31441 ttgcaccgtg agcgttacgg tgaagatacc ggtcatttcg gcatgattga cgccattgat
    31501 gatccgcagg attttgccgc gttgtttggc gcagcggaag cgtggttgaa gtcacaaggt
    31561 gcaagtaaga tcagcgggcc tttcagcctg aatatcaatc aggaaagcgg attactgatt
    31621 gaaggttttg acacaccacc ctgtgcgatg atgccacacg gcaaaccgtg gtatgccgcg
    31681 catattgaac aactgggtta tcacaaaggc attgatttac tggcgtggtg gatgcagcga
    31741 accgatctca ctttctctcc ggcgctaaaa aaactgatgg atcaggtgcg caaaaaggtg
    31801 accattcgct gcatcaatcg tcagcggttt gccgaagaga tgcagatcct gcgtgagatt
    31861 ttcaactctg gctggcagca caactgggga tttgtgccgt ttaccgaaca tgaattcgcg
    31921 accatgggcg accaacttaa atatctggtg ccggacgata tgatctacat cgctgagatt
    31981 gattctgcac cctgcgcgtt tattgtcggc ttgcctaaca tcaacgaggc gattgccgat
    32041 ctgaacggat cgctctttcc cttcggctgg gcaaaattgc tgtggcgttt gaaagtcagc
    32101 ggtgtgcgaa ctgcgcgagt accgctgatg ggcgtgcgcg acgagtatca gttcagccgc
    32161 atcggcccgg tgattgcgct gttattgatt gaagccttac gcgatccgtt tgcccgccgg
    32221 aagatcgatg cgctggagat gtaatggatc ctcgaaacca atacaggcat gaataacatg
    32281 ctggaacgca ttggggcgga gccgtacaag cgttaccgat tgtatgaaaa gcaaatttaa
    32341 taccagatcc tgtttagcgc aggggcggtc attcgggaga gggtatagat gaacagattt
    32401 aacaccagat cctgttaagt gcaggggcgg tcccctcgcc ttggggatag ggtaaaactc
    32461 tgctcacatt cccaccttgt tcatctccag cggcaccgcc gagcgaatca aatggtggcc
    32521 ttccagctcg atatcgtgga gattcttttt cacggtccgt acatgatcgc gcgctgcgcg
    32581 ctgagcgacg tgcggcagcc gctgcaacac cgcgttgtag atccgcgcat gctggcgatc
    32641 gatctgcttt ttttgctgtg gtcgatggta gagattattt acgctggcaa acactgaatt
    32701 aaacatcaga tcggtcaatg attgcagcgt aaacaccagc acctgattgt gagaagcctg
    32761 acaaatggcg agatggaaag cgtgatccaa ctgcgcatgt tcgatcagcg aaatctcttt
    32821 gtggttctca ctggcggcga gcattttttc ataacagcgg gttatcacaa caaaatcagc
    32881 ctgcgttccc agcgttgccg ccagccttgc cgattcgccc tccagtaatg cgcgaacgtc
    32941 gagcagatcg tacagcgttc gcggctgcgt actgaacaga tggatcagcg ggctggtgtc
    33001 ctgcacccga ttaagccgtg cgacatgaga atcgcgaccc tgcgccgttt caataatccc
    33061 gcgcccgcgc agcacggtca gcccttcacg cagtgcggag cgtgagaagc cgagcttttc
    33121 acacagtcga cgttccgagg gaagcggctg accgaccttc agtacgccgt cgataattaa
    33181 ccgttcgata ctctctgcaa ccacttcgca aatagggcga cgttcatctt tcattcccgg
    33241 acctcgtgca caggtaggac caatttttct gttcttaact cgcaaaacac gcacatcacg
    33301 caagtgatta tgttacatca atttaacatt aagttaacca agacaaggtc acagagctgg
    33361 aaaaaaaatg gtctgaccgg tagctaaaga gatagacgaa aacgaaaagc ccgcttaata
    33421 actgttcaca gaagcagcgc gcaaaaatca gctgccacac aacaaagcga agcctactca
    33481 tgagcatctt gtacgaagag cgtcttgatg gcgctttacc cgatgtcgac cgcacatcgg
    33541 tactgatggc actgcgtgag catgtccctg gacttgagat cctgcatacc gatgaggaga
    33601 tcattcctta cgagtgtgac gggttgagcg cgtatcgcac gcgtccatta ctggttgttc
    33661 tgcctaagca aatggaacag gtgacagcga ttctggctgt ctgccatcgc ctgcgtgtac
    33721 cggtggtgac ccgtggtgca ggcaccgggc tttctggtgg cgcgctgccg ctggaaaaag
    33781 gtgtgttgtt ggtgatggcg cgctttaaag agagcctcga cattaacccc gttggtcgcc
    33841 gcgcgcgcgt gcagccgggc gtatgtaacc tggcgatctc ccaggccgtt gcgccgcata
    33901 atctctatta cgcgccggat ccttcctcac aaatcgcctg ttcaattggc ggcaatgtgg
    33961 cggaaaatgc cggtggcgtc cactgcctga aatatggtct gaccgtacat aacctgctga
    34021 aaattgaagt gcaaacgctg gacggcgagg cgctgacgct gggatcggac gcgctggatt
    34081 cacctggttt tgacctgctg gcgctgttca ccggatcgga aggtatgctc ggcgtgacca
    34141 ccgaagtgac ggtaaaactg ctgccgaagc cgcccgtggc gcgggttctg ttagccagct
    34201 ttgactcggt agaaaaagcc ggacttgcgg ttggtgacat catcgccaat ggcattatcc
    34261 ccggcgggct ggagatgatg gataacctgt cgatccgcgc ggcggaagat tttattcatg
    34321 ccggttatcc cgtcgacgcc gaagcgattt tgttatgcga actggacggc gtggagtctg
    34381 acgtacagga agactgcgag cgggttaact acatcttgtt gaaagcgggc gcgactgacg
    34441 tccgtttggc acaggacgaa gcagagcgcg tacgtttctg ggccggtcgc aaaaatgcgt
    34501 tcccggcggt aggacgtatc tccccggatt actactgcat ggatggcacc atcccgcgtc
    34561 gcgccctgcc tggcgtactg gaaggcattg cccgtttatc gcagcaatat gatttacgtg
    34621 ttgccaacgt ctttcatgcc ggagatggca acatgcaccc gttaatcctt ttcgatgcca
    34681 acgaacttgg tgaatttgcc cgcgcggaag agctgggcgg gaagatcctc gaactctgcg
    34741 ttgaagttgg cggcagcatc agtggggaac atggcatcgg gcgagaaaaa atcaatcaaa
    34801 tgtgcgccca gttcaacagc gatgaaatca cgaccttcca tgcggtcaag gcggcgtttg
    34861 accccgatgg tttgctgaac cctgggaaaa acattcccac gctacaccgc tgtgctgaat
    34921 ttggtgccat gcatgtgcat cacggtcatt taccttttcc tgaactggag cgcttttgat
    34981 gctacgcgag tgtgattaca gtcaggcgct gctggagcag gtgaatcagg caattagcga
    35041 taaaacgccg ctggtgattc agggcagcaa tagcaaagcc tttttaggtc gcccagtcac
    35101 cgggcaaacg ctggatgtgc gttgtcatcg cggcattgtt aattacgacc cgaccgagct
    35161 ggtgataacc gctcgtgccg gaacgccgct ggtggcgatt gaagcggcgc tggagagcgc
    35221 cgggcaaatg cttccctgtg agccgccgca ttatggtgaa gaagccacct ggggcgggat
    35281 ggtcgcctgc gggctggcgg ggccgcgtcg cccgtggagc ggttcggtcc gcgattttgt
    35341 cctcggcacg cgcatcatta ccggcactgg aaaacatctg cgttttggtg gcgaagtgat
    35401 gaaaaacgtt gccggatacg atctctcacg gttaatggcc ggaagctacg gttgtcttgg
    35461 cgtgctcact gaaatctcaa tgaaagtgtt accgcgaccg cgtgcctctc tgagcctgcg
    35521 tcgggaaatc agcctgcaag aagccatgag tgaaatcgcc gagtggcaac tccagccatt
    35581 acccattagt ggcttatgtt acttcgacaa tgcgttgtgg atccgccttg agggcggcga
    35641 aggatcggta aaagcagcgc gtgaactgct gggtggcgaa gaggttgcgg gtcagttctg
    35701 gcagcaattg cgtgaacaac aactgccgtt cttctcgtta ccaggtacct tatggcgcat
    35761 ttcattaccc agcgatgcgc cgatgatgga tttacccggc gagcaactga tcgactgggg
    35821 cggggcgtta cgctggctga aatcgacagc cgatgacaat caaatccatc gcatcgcccg
    35881 caacgctggc ggtcatgcga cccgctttag tgccggagat ggtggctttg ccccgctatc
    35941 ggctccttta ttctgctatc accagcagct taaacagcag ctcgaccctt gcggcgtgtt
    36001 taaccccggt cgcatgtacg cggaactttg aggagcaggc tatgcaaacc cgattaaccg
    36061 aagagatgcg gcagaacgcg cgcgcgctgg aagccgacag catcctgcgc gcctgtgtcc
    36121 actgcggatt ttgtaccgca acctgcccaa cctatcagct tctgggcgat gaactggacg
    36181 ggccgcgcgg gcgcatctat ctgattaaac aggtgctgga aggcaacgaa gtcacgctta
    36241 aaacacagga gcatctcgat cgctgcctca cttgccgcaa ttgtgaaacc acctgtcctt
    36301 ctggtgtgcg ctatcacaat ttgctggata tcgggcgtga tattgtcgag cagaaagtga
    36361 aacgcccact gccggagcga atgctgcgcg aaggattgcg ccaggtagtg ccgcgtccgg
    36421 cggtcttccg tgcgctgacg caggtagggc tggtgctgcg accgttttta ccggaacagg
    36481 tcagagcaaa actgcctgct gaaacggtga aagctaaacc gcgtccgccg ctgcgccata
    36541 agcgtcgggt tttaatgctg gaaggctgcg cccagcctac gctttcgccc aacaccaacg
    36601 cggcaactgc gcgagtgctg gatcgtctgg ggatcagcgt catgtcagct aacgaagcag
    36661 gctgttgtgg cgcggtggac tatcatctta atgcgcagga gaaagggttg gcacgggcgc
    36721 gcaataatat tgatgcctgg tggcccgcga ttgaagcagg tgccgaggca attttgcaaa
    36781 ccgccagcgg ttgcggcgcg ttcgtcaaag agtatgggca gatgctgaaa aacgatgcgt
    36841 tatatgccga taaagcgcgt caggtcagtg aactggcggt cgatttagtc gaacttctgc
    36901 gcgaggaacc gctggaaaaa ctggcaattc gcggcgataa aaaactggcc ttccactgcc
    36961 cgtgtaccct acaacatgcg caaaagctga acggcgaagt ggaaaacgtg ttacttcgtc
    37021 ttggatttac cttaacggac gttcccgaca gccatctgtg ctgcggttca gcgggaacat
    37081 atgcgttaac gcatcccgat ctggcacgcc agctgcggga taacaaaatg aatgcgctgg
    37141 aaagcggcaa accggaaatg atcgtcaccg ccaacattgg ttgccagacg catctggcga
    37201 gcgccggtcg tacctctgtg cgtcactgga ttgaaattgt agaacaagcc cttgaaaagg
    37261 aataaaaaaa tgaaaactaa agtcattctt agccagcaaa tggcgagtgc aattattgcc
    37321 gcaggtcagg aagaggcgca gaaaaataac tggtctgttt ccattgctgt tgccgatgac
    37381 ggcggtcatc tgctggcgtt aagtcgcatg gacgattgcg cgccgattgc ggcttacatc
    37441 tcccaggaga aagcgcgtac cgccgcgctg gggcgtcgtg aaactaaggg ctatgaagag
    37501 atggtgaaca acggacgtac cgcgttcgtg actgcgccgt tactgacgtc gctggaaggc
    37561 ggcgtaccgg ttgttgttga tgggcaaatt attggtgccg tgggcgtttc tggtttaacc
    37621 ggagcacagg atgcgcaggt cgcgaaagcg gcagcagcgg tgttggcgaa ataagcgaaa
    37681 acgaggagag aaacaatgag tcaaaccata acccagggcc gtttacgcat tgacgccaat
    37741 tttaaacgtt ttgtggatgg agaagttttg ccgggcgtgg aactggacgc tgctgcattc
    37801 tggcacaatg ttgatgagat cgttcacgat ctggcaccag aaaatcgtca gttgctggca
    37861 gaacgcgatc gcattcaggc ggcgcttgat gagtggcatc gcagcaatcc ggggccggta
    37921 aaagataaag cggcctataa atctttcctt cgtgaactgg gctacctggt gccgcaaccg
    37981 gagcgcgtga cggtggaaac cacgggcatt gacagcgaaa tcaccagtca ggcggggcca
    38041 cagctggtgg ttccggcaat gaacgcccgc tacgcgctga acgcggcgaa cgctcgctgg
    38101 ggctcactgt acgatgcgtt atacggcagc gacatcatcc cgcaggaagg ggcgatggtc
    38161 agtggctacg atccgcagcg cggtgagcag gttatcgcct gggttcgacg tttcctcgat
    38221 gaatcactac cgctggaaaa cggcagctat caggatgtgg tggcgtttaa ggtggtcgat
    38281 aaacaattac gcatccagtt gaaaaatggt aaagaaacca cgttacgtac tccagcacag
    38341 tttgtcggtt accgtggcga tgccgctgcg ccgacctgca ttttgctgaa aaataacggc
    38401 ctgcatattg aactgcaaat tgatgccaac gggcggattg gcaaagacga tccggcgcac
    38461 atcaacgatg ttatcgtcga agctgctatc agtaccattc tcgactgcga agattcggtc
    38521 gcggcggttg atgcggaaga taaaatcctg ctgtaccgca atctgctggg cctgatgcag
    38581 gggactctgc aagagaaaat ggagaaaaac ggtcggcaaa tcgtacgtaa actgaatgac
    38641 gatcgtcagt acaccgccgc cgatggctct gaaatttctc tgcacggacg ctcgttgctg
    38701 tttatccgca acgtgggtca tttgatgacc attcctgtga tttgggacag cgaaggcaat
    38761 gaaatcccgg aaggtattct tgatggcgtc atgactggcg cgattgccct ctatgattta
    38821 aaagtgcaga aaaactcgcg cactggcagc gtctatattg tgaaaccgaa aatgcacggt
    38881 ccgcaggaag tggcgttcgc caacaaactg tttacccgca ttgagacaat gctcggtata
    38941 gtaccgaata ccctgaaaat gggcattatg gatgaagaac gccggacctc gctgaacttg
    39001 cggagctgta tcgctcaggc gcgcaaccgc gtggcgttca tcaataccgg tttcctcgac
    39061 cgtaccggcg atgaaatgca ttcggtgatg gaagctggcc cgatgctgcg taaaaaccag
    39121 atgaaatcga cgccgtggat caaagcctac gaacgtaata acgtgctttc cggtctgttc
    39181 tgtgggctgc gcggtaaagc gcaaatcggt aaaggcatgt gggcaatgcc ggacctgatg
    39241 gcagacatgt acagccagaa gggcgaccaa ctgcgtgccg gggcaaacac agcctgggtt
    39301 ccgtcaccaa ccgctgctac gctccatgcg ctgcactacc accaaaccaa cgtacagagc
    39361 gtgcaagcca acattgccca gaccgagttc aatgctgaat ttgaaccgct gctggacgat
    39421 ctgctgacta ttccggtggc tgaaaacgct aactggtcgg cgcaagagat ccaacaagag
    39481 ctggataaca acgtgcaggg gattctgggg tacgtggtgc gttgggtgga gcaggggatt
    39541 ggttgttcaa aagtgccgga tattcacaac gtggcgctga tggaagaccg cgcaacgctg
    39601 cgtatctcca gccagcatat cgccaactgg ttacgtcacg gtattctgac caaagaacag
    39661 gtgcaggcgt cgctggagaa tatggcgaaa gtggttgatc agcaaaatgc tggcgatccg
    39721 gcttatcgtc cgatggcggg gaatttcgct aactcgtgtg cttttaaagc tgccagcgat
    39781 ttaatcttcc tcggcgtgaa acagccaaac ggttataccg aaccgttatt acacgcctgg
    39841 cgtttacgcg aaaaagaaag tcattaatag cgtcaaaaaa gccccggcaa gttttattgt
    39901 cggggctatg atagtaactg cggtagtaca tttatcccgg aaataaccgg agcgattatc
    39961 agggtttaac aatattaaat acccgtagcg cattactagc gggcctaaaa caacaatatg
    40021 gacatcttaa cgatactctg cgcctgcgtg tgcggaacag aaacccttac ccgatatact
    40081 tgttatgcca gaggtagcaa ttgcgttagc ggcgtactgt tgctgtaaag tataacgcct
    40141 atattttaca gttcagtcct cttttgctta ttacaacctt aagtatatca agcatataaa
    40201 gataataaga gactgaacaa tatggttacc tggacccaaa tgtatatgcc gatgggagga
    40261 ctggggctat ccgctctggt cgccctgatc ccgataatat tcttcttcgt tgcactcgcg
    40321 gtattacgtc tgaaaggaca tgtcgctgga gcaataaccc ttatattatc tatcctgatt
    40381 gcgatattcg cctttaaaat gccgattgat atggcatttg ctgctgcggg ctatggcttt
    40441 atttatggat tatggccaat tgcgtggatt attgtcgcgg cggtgttcct gtataaatta
    40501 accgttgcca gcgggcagtt cgatattatc cgcagctcgg ttatctccat caccgacgat
    40561 cagcgtttgc aggtgttact gattggtttc tcctttggtg cgttgctgga aggagcggct
    40621 ggctttggtg cgccggtggc gattaccggt gcgctgctgg tgggcctggg cttcaaaccg
    40681 ttatacgcgg cggggctgtg tctgattgcc aatactgcgc cggtggcgtt tggtgcgttg
    40741 ggcgtgccga ttctggtcgc cggtcaggta acgggaatcg atccgttcca cattggcgca
    40801 atggcgggac gtcagttacc gttcctgtcg gttcttgtgc cgttctggct ggtagcaatg
    40861 atggacggct ggaaaggggt gaaagagacg tggccagcgg cgctggttgc tgggggaagc
    40921 ttcgctgtca ctcagttctt tacctctaac tatattggtc cggaactgcc ggatattact
    40981 tcggcgctgg tgagtatcgt ctcactcgct ttattcctta aagtctggcg gccgaaaaat
    41041 accgaaacgg caatcagcat ggggcaatcc gcaggtgcga tggtggtaaa taagccatct
    41101 tctggcggtc ccgtgccttc agaatatagt ctggggcaaa tcattcgtgc gtggtcaccg
    41161 tttttaatct taacggtgct ggtcaccatc tggaccatga agccgtttaa agcgttattt
    41221 gctccgggcg gcgcgtttta ttcactggtg attaatttcc agatccctca tttgcatcaa
    41281 caagtgttga aagcggcacc cattgtcgcc caaccaacgc caatggatgc ggtgtttaaa
    41341 ttcgaacctc tctcggctgg cggcaccgct atttttattg cggcgattat ctctatcttc
    41401 atcctcggcg tgggcatcaa gaaagggatt ggcgtctttg ccgaaacgct aattagtctg
    41461 aagtggccga tattgtcgat tggcatggtg ctggcattcg ccttcgtcac caactattcc
    41521 ggcatgtcca ccacgctggc gctggtactg gcaggtacag gcgtgatgtt cccgtttttc
    41581 tcaccgtttc tcggctggct gggcgtattc cttaccgggt cggacacttc ttcaaacgcc
    41641 ctgtttggtt cactgcaatc gaccacggcg cagcaaatca acgtctctga cactctgctg
    41701 gtggcagcaa ataccagcgg cggcgtaact ggcaagatga tctccccgca atctatcgcc
    41761 gtggcctgcg ccgcgacggg catggtgggc cgagaatctg aactgttccg ctacaccgtg
    41821 aagcacagtc tgatttttgc cggcgttatc ggcgttatca ccctgctgca ggcgtatgtg
    41881 tttaccggga tgttagtctc gtaaatcaat aaagccggat gaaggtaaat atcacccggc
    41941 tttccatttg tctgccgttt atctcgccag ggaaccgttt ttacgattgg cttctttata
    42001 cgtagtctcc gtttcacgat gcgaattgtt taatatttat acaacttggc ttctgtgctg
    42061 gttataaagt acccactttc atgagatcaa ttttagtatg ttattacatt gcttcatctg
    42121 ttgctttcat gacgaatggc gttttttctc ttatttatat ttttcactag aacaatgagt
    42181 agatcaaaat tcccaaaact cccaaaaaca taagattatt cgcaaagctg attttgcaga
    42241 ataatgagaa cttttaagat ttgtcgagga tcaattttaa gcaatttatc tccacttgtt
    42301 acataaaaga tgccgttagg ttaataagtt aatcttgcta taacagtgtt cgttactgcc
    42361 tattaataaa gatatttggg atagggtaac gtggatgagg attcgaaatc atccgtcttg
    42421 cggaaagtga aatgttggca gatgttaata ttatgtatgt attttaactc caacaacaca
    42481 ccaatcattt tgttagaaat aagaaaattt atggttaaat aatttggtga ttatgaagca
    42541 ttaaatcgta atgccaaata ttgttcgctt tggagagtat tattgcctgt ttgtgtctgt
    42601 tgagcagaaa aatcatctga atttgaatac ttttattagg cgcgaagaaa tgaaaaaatc
    42661 attaaaatta ttaatttata tgaatatttc agagttggag ttcgaatgtt gacacctgag
    42721 tatttcctgc taaaaatagc cctgctttac ggatggttgt attgcaataa cacgaaagta
    42781 gtaagagatt aaaggtatct gtcagcgata actctgcaag tgatgacatt ttgtaaacca
    42841 aataatgtaa atcagcaatg tatcttttca cttaattata acaaatgtgg tattcactaa
    42901 gaatctgata cgagcttaca tcacgactat tttagtctca tcgcgggata cagggatgca
    42961 ccgatggatc tcagcacacc gtaatgcaat acctgaacaa atagaacagt aaagtggttc
    43021 agtattaatg aaagctctta ttttccgttg ggaaaactta aaatagatga gaaggctttt
    43081 tgatttctta tgtgatctca tttcgagtat tgagaaattt gtgattttta aatttttcaa
    43141 taagccctgt aagggattgg ggttttatga gaaataaaga gacgtattgt ctttgtttaa
    43201 acggtac
//
LOCUS       DAJTNM010000017        43171 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTNM010000017 DAJTNM010000000
VERSION     DAJTNM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.017"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..413
                     /locus_tag="OO172_001085"
     CDS             <1..413
                     /locus_tag="OO172_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5511348.1"
                     /translation="MYIGAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKL
                     FWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFD
                     KYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            438..3037
                     /locus_tag="OO172_001086"
                     /pseudo
     CDS             438..3037
                     /locus_tag="OO172_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3028..4098
                     /locus_tag="OO172_001087"
     CDS             3028..4098
                     /locus_tag="OO172_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5511349.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQVIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4110..4652
                     /locus_tag="OO172_001088"
     CDS             4110..4652
                     /locus_tag="OO172_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5511350.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGAGVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIQLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4660..5175
                     /locus_tag="OO172_001089"
     CDS             4660..5175
                     /locus_tag="OO172_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5511351.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTLDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5141..5878
                     /locus_tag="OO172_001090"
     CDS             5141..5878
                     /locus_tag="OO172_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5511352.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5989..6999
                     /gene="pyrD"
                     /locus_tag="OO172_001091"
     CDS             5989..6999
                     /gene="pyrD"
                     /locus_tag="OO172_001091"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5511353.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7173..7715
                     /gene="zapC"
                     /locus_tag="OO172_001092"
     CDS             7173..7715
                     /gene="zapC"
                     /locus_tag="OO172_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5511354.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="OO172_001093"
     CDS             complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="OO172_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5511355.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9065..11173
                     /gene="rlmKL"
                     /locus_tag="OO172_001094"
     CDS             9065..11173
                     /gene="rlmKL"
                     /locus_tag="OO172_001094"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5511356.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11185..13092
                     /locus_tag="OO172_001095"
                     /pseudo
     CDS             11185..13092
                     /locus_tag="OO172_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13222..14475
                     /gene="pqiA"
                     /locus_tag="OO172_001096"
     CDS             13222..14475
                     /gene="pqiA"
                     /locus_tag="OO172_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5511357.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAVVIFLASIMVPTLKMSAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14480..16120
                     /gene="pqiB"
                     /locus_tag="OO172_001097"
     CDS             14480..16120
                     /gene="pqiB"
                     /locus_tag="OO172_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5511358.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16117..16680
                     /gene="pqiC"
                     /locus_tag="OO172_001098"
     CDS             16117..16680
                     /gene="pqiC"
                     /locus_tag="OO172_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5511359.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16936..17103
                     /gene="rmf"
                     /locus_tag="OO172_001099"
     CDS             16936..17103
                     /gene="rmf"
                     /locus_tag="OO172_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5511360.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="OO172_001100"
     CDS             complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="OO172_001100"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5511361.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKIT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17760..19520)
                     /locus_tag="OO172_001101"
     CDS             complement(17760..19520)
                     /locus_tag="OO172_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5511362.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19705..20157
                     /gene="matP"
                     /locus_tag="OO172_001102"
     CDS             19705..20157
                     /gene="matP"
                     /locus_tag="OO172_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5511363.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="OO172_001103"
     CDS             complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="OO172_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5511364.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="OO172_001104"
     CDS             complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="OO172_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5511365.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22363..22992
                     /gene="sxy"
                     /locus_tag="OO172_001105"
     CDS             22363..22992
                     /gene="sxy"
                     /locus_tag="OO172_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5511366.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="OO172_001106"
     CDS             complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="OO172_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5511367.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25127..25573)
                     /locus_tag="OO172_001107"
     CDS             complement(25127..25573)
                     /locus_tag="OO172_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5511368.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25696..27750
                     /gene="helD"
                     /locus_tag="OO172_001108"
     CDS             25696..27750
                     /gene="helD"
                     /locus_tag="OO172_001108"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5511369.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSDVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="OO172_001109"
     CDS             complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="OO172_001109"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5511370.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28336..28998)
                     /locus_tag="OO172_001110"
     CDS             complement(28336..28998)
                     /locus_tag="OO172_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5511371.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29171..29584
                     /locus_tag="OO172_001111"
     CDS             29171..29584
                     /locus_tag="OO172_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5511372.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="OO172_001112"
     CDS             complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="OO172_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5511373.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="OO172_001113"
     CDS             complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="OO172_001113"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5511374.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31289..31567
                     /gene="yccX"
                     /locus_tag="OO172_001114"
     CDS             31289..31567
                     /gene="yccX"
                     /locus_tag="OO172_001114"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5511375.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="OO172_001115"
     CDS             complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="OO172_001115"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5511376.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="OO172_001116"
     CDS             complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="OO172_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5511377.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32850..32937)
                     /locus_tag="OO172_001117"
     tRNA            complement(32850..32937)
                     /locus_tag="OO172_001117"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32901..32903),aa:Ser,seq:tga)
     gene            33363..34481
                     /gene="hyaA"
                     /locus_tag="OO172_001118"
     CDS             33363..34481
                     /gene="hyaA"
                     /locus_tag="OO172_001118"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5511378.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLTKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34478..36236
                     /gene="hyaB"
                     /locus_tag="OO172_001119"
                     /pseudo
     CDS             34478..36236
                     /gene="hyaB"
                     /locus_tag="OO172_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36255..36962
                     /gene="hyaC"
                     /locus_tag="OO172_001120"
     CDS             36255..36962
                     /gene="hyaC"
                     /locus_tag="OO172_001120"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5511379.1"
                     /translation="MQQKSDNIVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36959..37546
                     /locus_tag="OO172_001121"
     CDS             36959..37546
                     /locus_tag="OO172_001121"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5511380.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37543..37941
                     /gene="hyaE"
                     /locus_tag="OO172_001122"
     CDS             37543..37941
                     /gene="hyaE"
                     /locus_tag="OO172_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5511381.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37938..38795
                     /locus_tag="OO172_001123"
     CDS             37938..38795
                     /locus_tag="OO172_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5511382.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38929..40473
                     /gene="appC"
                     /locus_tag="OO172_001124"
     CDS             38929..40473
                     /gene="appC"
                     /locus_tag="OO172_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5511383.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40485..41621
                     /gene="appB"
                     /locus_tag="OO172_001125"
     CDS             40485..41621
                     /gene="appB"
                     /locus_tag="OO172_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5511384.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41633..41725
                     /gene="cbdX"
                     /locus_tag="OO172_001126"
     CDS             41633..41725
                     /gene="cbdX"
                     /locus_tag="OO172_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5511385.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41805..43103
                     /gene="appA"
                     /locus_tag="OO172_001127"
     CDS             41805..43103
                     /gene="appA"
                     /locus_tag="OO172_001127"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5511386.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
BASE COUNT        10744 a        10502 c        11497 g        10428 t
ORIGIN      
        1 ttatgtacat tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac
       61 gggcagtccc ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa
      121 gcgtcatcaa acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg
      181 cgaaactgcg ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt
      241 tcattaccct gacagacctg aaaataggtg gaaaagtagt taaaaatcaa atgatttccc
      301 cctttgataa ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc
      361 gaaccatcaa tgactacggg gcggaaacgc cgcaactcaa ctgtaaatcg taagccgtct
      421 tcagttaaga gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg
      481 gagtgccatc gtttattggt gggctggtgg tgtttgtgtc ggcagcgttc aatgcacaag
      541 ctgaaacctg gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt
      601 ctcgtttcga aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga
      661 atcagacaat tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga
      721 ttgccgcctg tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc
      781 cggcttttaa acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg
      841 ccagcgtaac gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca
      901 tcaaaagtaa tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc
      961 tattactggg atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt
     1021 ctggcgacag ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc
     1081 gcaacaattc aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca
     1141 gctatttgca gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata
     1201 ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca
     1261 acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca
     1321 atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg
     1381 gtgcttttga aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa
     1441 tcaaagaagc ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac
     1501 tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta
     1561 atgaacagca ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa
     1621 cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg
     1681 gatttaacct tggtgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc
     1741 tggcagagca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca
     1801 accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca
     1861 ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg
     1921 aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc
     1981 aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc
     2041 aaacttactg gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa
     2101 ttaaagatct ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg
     2161 ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta
     2221 atgatagcta taccaggaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa
     2281 cgaagggcac attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct
     2341 gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag
     2401 catggactac aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg
     2461 cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat
     2521 taccctgggg caatcgctgg gggaaactaa cgtgctgatt gtagcaccag gcgcagagaa
     2581 tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc
     2641 ctatgccact tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa
     2701 cgtggatctt gaaaatgcag tagtcaacgt ggttcccacc aaaggggcgt tggttctggc
     2761 ggagttcaat gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc
     2821 gctgcgtttt ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga
     2881 tgatgatggc tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg
     2941 gggcgaagct cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa
     3001 ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta
     3061 ttactacgac tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat
     3121 atcgggcagt gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa
     3181 actctggatg aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac
     3241 gaatcgagtg attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga
     3301 tgggcattcg ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat
     3361 gactatcttg ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct
     3421 ttcgaaaacg tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa
     3481 cgtttaggtg gccagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag
     3541 ccgttcgttg gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac
     3601 aacatacccg caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg
     3661 tctggtacga tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt
     3721 gatctgggcg acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc
     3781 agaacggcag aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac
     3841 gtcaatctga gtctgacggc aacctcagac cccagctatc cccaggtgat taagacgtca
     3901 cgtcctggcg tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt
     3961 gggacactac cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat
     4021 ccagtcagca cgacaggcgt accaccagaa accgggcgat ttgaagccac ggcaacggtg
     4081 agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta
     4141 ttttgttctt tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta
     4201 tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac
     4261 aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca
     4321 ctgctgctga tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcgggcg
     4381 tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag
     4441 gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc
     4501 aagaaatcca gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac
     4561 tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg
     4621 cggttctcta ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg
     4681 attctgttct tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc
     4741 aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc
     4801 aatgtgcagt taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag
     4861 aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc
     4921 ttttcaggaa ccctcgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat
     4981 gcagccagtg ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac
     5041 caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa
     5101 ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta
     5161 cttcattatg actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct
     5221 gctggtggcg ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc
     5281 ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat
     5341 taacagcaaa atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc
     5401 actattactg gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg
     5461 cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag
     5521 cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc
     5581 ggtatttaaa ctgttctggc gacacgaagg tttgccgggc gacccgctgg aagcttatca
     5641 acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta
     5701 cattaacctg attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc
     5761 gcctaaaagc cagcgtcaga caaactggtg tcaggtaata gcaccgtgtc atgtcgcctg
     5821 gcggacgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa
     5881 caggttctga aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact
     5941 cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc
     6001 ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag
     6061 caattacgcc gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg
     6121 aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt
     6181 gataaagacg gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc
     6241 ggtaccgtca cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta
     6301 gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta
     6361 gagaacgtaa aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa
     6421 gatacgccag tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc
     6481 tatgcgggat atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg
     6541 caatatggtg aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg
     6601 caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa
     6661 gacgaattga tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca
     6721 accaatacca ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt
     6781 ggcttaagtg gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta
     6841 gaattaaacg gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg
     6901 cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa
     6961 ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt
     7021 tataagataa tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt
     7081 atttagacat tttgtacttt tattgttgag gttattaagc gaagcaacaa tggattgtgt
     7141 tgctgcggtt ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc
     7201 gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat
     7261 ttcgctcacg ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt
     7321 gcgttgatga tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat
     7381 ctaaagacca aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac
     7441 cgcaaatgcc gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg
     7501 gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg
     7561 aatctggcga aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc
     7621 gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga
     7681 atgttgacag cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg
     7741 gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg
     7801 tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat
     7861 aagggatacg aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac
     7921 gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg
     7981 cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca
     8041 cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga
     8101 catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg
     8161 gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa
     8221 ccacatcaaa caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg
     8281 acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac
     8341 gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa
     8401 atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc
     8461 gtaattgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa
     8521 ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag
     8581 caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag
     8641 gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag
     8701 gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg
     8761 taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca
     8821 cggtgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct
     8881 ataatgcgca acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg
     8941 ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc
     9001 catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga
     9061 cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga
     9121 actggaaaat ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg
     9181 cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt
     9241 gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat
     9301 caactggaca gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa
     9361 tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc
     9421 tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt
     9481 taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt
     9541 acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc
     9601 cgctattgtg atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg
     9661 ttccggtacg ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca
     9721 ccgtgggcgt tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt
     9781 gaaagcggaa gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta
     9841 cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg
     9901 gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc
     9961 gaaagggccg tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga
    10021 accggcgctg attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg
    10081 ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc
    10141 agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt
    10201 tgccgaaagc acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct
    10261 gcgtaagaac ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg
    10321 cctgtatgac gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt
    10381 ggtggtgcag gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct
    10441 gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct
    10501 gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga
    10561 gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga
    10621 tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg
    10681 caaagatttc ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg
    10741 cggtgcacgc agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg
    10801 caacctgcgt ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct
    10861 ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt
    10921 ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat
    10981 gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg
    11041 tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac
    11101 gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat
    11161 taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg
    11221 ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt
    11281 tgtctggtgg gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa
    11341 caagggctgg atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa
    11401 caggatccgc cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa
    11461 gaacaagcgg aatatctgaa acgctatcac gatatttcgt gcctggtgat gaacgacccg
    11521 agcgagaaaa atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg
    11581 tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt
    11641 gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg
    11701 agcaatccgc gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc
    11761 gactggctgg aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac
    11821 cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg
    11881 acctatccag ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa
    11941 gaactacaaa atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgctag
    12001 gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt
    12061 cgcgaacgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc
    12121 agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag
    12181 caactggtga aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt
    12241 ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcgggc
    12301 agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg
    12361 gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg
    12421 gttaatggca agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa
    12481 cgggcgatga cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg
    12541 cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat
    12601 gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg
    12661 gttagccacg atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc
    12721 ggcggtaaaa ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa
    12781 tatgtggcac tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca
    12841 gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag
    12901 ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg
    12961 gatgcttcct tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct
    13021 gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa
    13081 aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg
    13141 cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata
    13201 gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc
    13261 tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt
    13321 ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc
    13381 tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg
    13441 aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct
    13501 gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt
    13561 tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag
    13621 caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg
    13681 ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc
    13741 tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt
    13801 cgctggttat gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc
    13861 gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct
    13921 gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg
    13981 cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg
    14041 cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc
    14101 attctgttat ggagcgaagg ctcttatccc gtcgctgtgg tgatctttct ggccagtatt
    14161 atggtgccaa cgttaaagat gagcgccatc gcgtggctgt gttgggatgc caaagggcat
    14221 ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc
    14281 tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt
    14341 ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca
    14401 atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag
    14461 catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa
    14521 gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct
    14581 tttttatcat tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg
    14641 aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag
    14701 cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat
    14761 ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga
    14821 agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc
    14881 gaaaggcagc aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga
    14941 tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga
    15001 tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca
    15061 aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag
    15121 caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg
    15181 tgtggagatg ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga
    15241 aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca
    15301 gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga
    15361 ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac
    15421 cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg
    15481 tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc
    15541 ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac
    15601 cggaaacctg gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc
    15661 aataaccggt attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct
    15721 ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa
    15781 tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct
    15841 gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc
    15901 gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg
    15961 ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga
    16021 actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa
    16081 ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt
    16141 gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta
    16201 cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta
    16261 gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat
    16321 gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc
    16381 aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct
    16441 ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat
    16501 ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt
    16561 ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg
    16621 gccggtgtct ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa
    16681 ttataaagat ttgtaaatat aaccgtctcc ggtatgccgc ctgaggcggt ttttttgttt
    16741 ctaacgtgcg gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta
    16801 gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta
    16861 ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta
    16921 tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt
    16981 ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat
    17041 caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc
    17101 tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct
    17161 gtctgatgat aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc
    17221 ggtatagatc agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg
    17281 acgattaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac
    17341 ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag
    17401 ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat
    17461 aaccggatcg ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc
    17521 tgcttcaaca taccctttgt cgaagttacc acccgtttcg gttattttga ccacacggtc
    17581 catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg
    17641 accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt
    17701 aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat
    17761 cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc
    17821 tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg
    17881 ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag
    17941 atggtgaatt tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga
    18001 acgttagcag tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata
    18061 gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga
    18121 tcgactgaac ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc
    18181 agggcgcaga gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca
    18241 aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac
    18301 gcctgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg
    18361 atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca
    18421 aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt
    18481 tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg
    18541 aggaaacctt cgcgccattc acgctgctgc agcattaaat ttagctgctc gccggagaag
    18601 gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca atgcgggcta
    18661 agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa
    18721 gcatccgctc ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac
    18781 cactggctta ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata
    18841 gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct
    18901 accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac
    18961 tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac
    19021 agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga
    19081 tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt
    19141 tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct
    19201 gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc
    19261 agttggcctg catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca
    19321 gactcttccg gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc
    19381 tccagcgcaa attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc
    19441 aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat
    19501 gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata
    19561 ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa
    19621 agctcacagg atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag
    19681 ttacacggta acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt
    19741 tggaaatgga agtatctggt aaagaagcat cgcgaagggg agttaattac ccgttacata
    19801 gaagccagtg ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg
    19861 ctggtaaatg gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag
    19921 accattcggg ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa
    19981 tcgatcgatc tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt
    20041 aaaacgcttt ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac
    20101 gcgaataaaa tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg
    20161 acgaaagtca gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta
    20221 ccagacgata acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat
    20281 ctctacgcga cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc
    20341 acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc
    20401 cgggatacct ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc
    20461 ctggttgtaa gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc
    20521 tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg
    20581 tttcagggtt gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt
    20641 ctgtacttcc ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg
    20701 gtaggaaaca cccaagctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc
    20761 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    20821 ctcaacacca cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc
    20881 accaggtacg ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat
    20941 gtccagatcg tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt
    21001 gtatgcgccg ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat
    21061 ttcaaaacca acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt
    21121 ttcgtgggtc ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag
    21181 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    21241 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    21301 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc
    21361 tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa
    21421 gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt
    21481 cttgtataag gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa
    21541 accgcttcag acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    21601 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
    21661 ttagcccgga aagttgtctc gttgtgtgag aggatgcgct taccggacgc ataataaacc
    21721 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
    21781 catctgtcaa ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg
    21841 actccactgt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
    21901 cagatgcctg aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc
    21961 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
    22021 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
    22081 gcgcaatttt acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt
    22141 acataatcaa tccagcccct gtgagttact gtatggatgt gcagtacatc cagtgacaac
    22201 aaagatcaac cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac
    22261 ataaattaat cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta
    22321 atgtgaatca ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat
    22381 aagcggatct ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca
    22441 ttgtttggca gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt
    22501 gagttgtatc ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc
    22561 tggctgacat ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa
    22621 agtctatggc gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg
    22681 ctgaaagaga aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct
    22741 tttcatctgg aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata
    22801 cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag
    22861 attctgttta tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca
    22921 cgccgccagg agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg
    22981 gagcttgagt aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc
    23041 caacttgttg tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc
    23101 gctgtttcag gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta
    23161 acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta
    23221 actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca
    23281 gcaagcgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg
    23341 ataccacaga cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg
    23401 ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt
    23461 tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcgggccaga
    23521 tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta
    23581 acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga
    23641 acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa
    23701 ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg
    23761 cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt
    23821 ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta
    23881 tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga
    23941 gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg
    24001 ggctgtcatc agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg
    24061 attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg
    24121 ttttgagtaa atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat
    24181 gcgtaaaagc gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc
    24241 gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca
    24301 cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac
    24361 gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac
    24421 cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca
    24481 gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag
    24541 gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca
    24601 ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt
    24661 accagacggc accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg
    24721 ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt
    24781 gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata
    24841 gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg
    24901 taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg
    24961 ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg
    25021 ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccaag
    25081 tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac
    25141 gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa
    25201 ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga
    25261 ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga
    25321 aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag
    25381 ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag
    25441 tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag
    25501 tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca
    25561 aaacggtacg cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac
    25621 gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg
    25681 tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac
    25741 ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa
    25801 taccttattc ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc
    25861 gagctggaat ttgtactgcc ggacgaaaaa gtggtgcgtc tgcacggcac agaatggggc
    25921 gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg
    25981 agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga
    26041 gaaaataaat ggctgacgcg tgagcaaacc tctgacgttc agcaacaaat ccgccaggct
    26101 ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
    26161 tggcgtaaat gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag
    26221 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
    26281 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
    26341 gccggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg
    26401 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
    26461 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
    26521 catgcgctgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
    26581 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
    26641 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
    26701 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
    26761 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
    26821 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
    26881 ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt
    26941 catcaggcga ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg
    27001 gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag
    27061 caaaacagtc agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc
    27121 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc
    27181 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt
    27241 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
    27301 aaagctgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
    27361 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
    27421 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
    27481 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtgat
    27541 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
    27601 gatttcccgg acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat
    27661 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat
    27721 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
    27781 attacttcag acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt
    27841 cgttgaaatg cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa
    27901 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
    27961 ttagtggatc ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa
    28021 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
    28081 gggaaatcaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
    28141 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
    28201 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac
    28261 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
    28321 acaggcacaa aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg
    28381 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
    28441 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
    28501 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
    28561 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
    28621 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
    28681 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
    28741 aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
    28801 cgtcttctca acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc
    28861 accgcgcagc agagagctgg aaaccttttt gccgtcgagt accagaagat cgacatcggt
    28921 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
    28981 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
    29041 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
    29101 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
    29161 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
    29221 gctggtgggg gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact
    29281 cgaccagggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
    29341 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
    29401 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac
    29461 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
    29521 gttaaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa
    29581 ataaaaaatc cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag
    29641 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
    29701 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
    29761 taggccgtta tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt
    29821 caccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
    29881 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
    29941 aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc
    30001 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat
    30061 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc
    30121 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
    30181 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt
    30241 ataaccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
    30301 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
    30361 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
    30421 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
    30481 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
    30541 gtagcggcgg gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc
    30601 gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagca gggcaggcgg
    30661 caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac
    30721 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc
    30781 cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg
    30841 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
    30901 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
    30961 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
    31021 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
    31081 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
    31141 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
    31201 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
    31261 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta
    31321 cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg
    31381 cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
    31441 agaagggcag gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg
    31501 tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat
    31561 ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
    31621 gcctttcggg aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt
    31681 cgccatcgct tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc
    31741 acgtacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc
    31801 aatcaccact gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc
    31861 cgtttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
    31921 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
    31981 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
    32041 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt
    32101 gctggtttca aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag
    32161 atgcagagca ggcagttgca ggaagatatt agcaaccata ccaatcagta ccaccacaat
    32221 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
    32281 agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat
    32341 tccggcagac agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa
    32401 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
    32461 accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact
    32521 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
    32581 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
    32641 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
    32701 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
    32761 tatcaggtca ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg
    32821 ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
    32881 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
    32941 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
    33001 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac
    33061 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta
    33121 aagaaaaata ggtccatttt tatcgctaaa agataaatcc acacagtttg tgttgttttg
    33181 tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa
    33241 ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg
    33301 tcgcttgccg accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg
    33361 atatgaataa cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca
    33421 gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac
    33481 caaagattgc ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc
    33541 tggaatgtac ctgctgtacc gaatctttta tccgctccgc tcacccactg acgaaggacg
    33601 tcatcctttc cctgatttcc ctctattacg acgataccct gatggctgcc gccggaaccc
    33661 aggcggaaga agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag
    33721 tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt
    33781 ttattgagaa actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct
    33841 gcgcgtcctg gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg
    33901 acaaagtcat caccgacaaa cccattatca aagtacctag ctgcccgccg atcccggatg
    33961 tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa
    34021 tgggtcgtcc actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc
    34081 acttcgacgc cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt
    34141 gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct
    34201 ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa
    34261 atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta
    34321 ctcattccac cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg
    34381 gtgtgcacgc agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag
    34441 aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca
    34501 gggatacacc atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga
    34561 aggccacatg cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc
    34621 ctgcggcacc atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc
    34681 gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt
    34741 ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa
    34801 cattatgctg acaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg
    34861 gatggactgg atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact
    34921 ggcgcaaagt ctctcctctt ggccgaaatc atcccctagc tatttcttcg acgtacaaaa
    34981 tcgcctgaag aaattcgttg aaggcgggca gttggggatc ttccgcaatg gctactgggg
    35041 gcacccgcag tacaaattgc cgctagaagc caacctgatg ggctttgccc actatctcga
    35101 agctctcgat ttccaacgtg aaattgtcaa aattcacgcg gtctttggcg gtaaaaaccc
    35161 gcatccaaac tggattgtcg gcgggatgcc ttgcgccatt aacattgacg aaagcggcgc
    35221 ggtcggggcg gtcaatatgg aacgcctgaa cctggtgcag tcgattatca cccgcacggc
    35281 agacttcatt aacaacgtga tgatccccga cgccttagcc atcggtcagt tcaacaaacc
    35341 gtggagcgaa atcggcacgg gtctttctga taaatgtgtc ctcagctacg gcgcattccc
    35401 ggatattgcc aacgactttg gtgagaaaag tctgctgatg cctggcggcg cggtgattaa
    35461 cggcgacttc aacaatgtgc tgccagtgga tttggttgat ccgcagcagg tgcaggagtt
    35521 tgtcgatcac gcctggtatc gttatcccaa cgatcaggtc gggcgtcatc cattcgatgg
    35581 cattaccgac ccgtggtaca accccggcga tgtcaaaggc agcgatacca acattcagca
    35641 gctgaatgaa caggaacgct actcgtggat caaagcgcca cgctggcgcg gtaacgcgat
    35701 ggaagtgggg ccgctggcac gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt
    35761 tgagtcggtc gatcgcatga tgtcggcgtt gaacctgccg ctttccggta tccagtcaac
    35821 gttgggccgc aggtaagttg cagtatttct tcgacaagct gatgaccaac ctgaaaaatg
    35881 gcaatctcgc cactgcttcc actgaaaaat gggaacctgc aacctggccg acagagtgcc
    35941 gtggcgtcgg ctttaccgaa gcgccgcgcg gagcgttagg ccactgggcc gccattcgcg
    36001 atggcaagat tgatctctac cagtgcgtgg tgccgaccac ctggaacgcc agcccgcgcg
    36061 atcccaaagg gcagattggc gcttatgaag cggcgctgat gaacaccaaa atggcgatcc
    36121 ccgagcaacc gctggagatc ctgcgtactc tgcacagctt tgatccgtgc ctcgcctgtt
    36181 caactcatgt gctgggcgac gacggtagcg agctgatctc cgtgcaggtg cgttaacagc
    36241 gaaggagaat catcatgcaa cagaaaagcg acaacattgt cagccactat gtctttgaag
    36301 caccagttcg tatctggcac tggctgacgg tgttatgcat ggcggtgttg atggtcaccg
    36361 gttactttat cggcaagccg ctaccttccg tcagtggcga ggcgacgtat ctgttctata
    36421 tgggttacat caggttaatt cacttcagcg ccgggatgat ttttaccgtg gtcttgctga
    36481 tgcggatcta ctgggctttt gttggcaatc gttactcccg cgagctgttt atcgtgccgg
    36541 tatggcgtaa aagctggtgg cagggcgtgt ggtatgaaat ccgctggtat ctgtttctgg
    36601 caaaacgtcc gagtgccgat ataggccata accccatcgc ccaggcggcg atgttcggct
    36661 atttcctgat gtcggtcttt atgatcatca ccggttttgc gctgtacagc gaacacagcc
    36721 agtacgctat ttttgcgccg ttccgttatg tggtggaatt tttctactgg acgggtggca
    36781 actcaatgga cattcacagc tggcatcggc tggggatgtg gctgattggg gcgtttgtga
    36841 tcggtcatgt ctacatggcg ctgcgtgaag acatcatgtc cgacgacacg gtgatctcca
    36901 ccatggtcaa cggctaccgt agccacaaat ttggcaaaat aagtaacaag gagcgttcat
    36961 gagcgagcaa cgcgtggtgg tcatggggct gggcaacctg ctgtgggccg atgaaggctt
    37021 cggcgtgcgg gtggcggaac ggctgtatgc ccattaccac tggcccgagt atgtggagat
    37081 tgtcgatggc ggtactcagg gactgaactt gctggggtat gtcgaaagcg ccagccatct
    37141 gttgattctc gatgccattg actacgggct ggaacctgga aggctgcgaa cctatgccgg
    37201 agaacgcatt ccggcctatc tcagcgcgaa gaaaatgagc ctgcatcaga acagtttctc
    37261 cgaagtgctg gcgctggcag atatccgcgg acatctgcca gcacatattg ccctcgtcgg
    37321 gctgcaaccc gcgatgctcg acgactacgg cggcagcctg agcgaactgg cacgggagca
    37381 actgcccgct gcggaacaat cggcgctggc gcagttggct gcgtggggaa gtgtgccgca
    37441 accggctaat gaatcgcgtt gtctcaatta tgactgtctg tcgatggaaa attacgaagg
    37501 cgttcgcttg cgccagtacc ggatgacaca ggaggagcag ggatgagcaa cgacacgcca
    37561 tttgatgcgt tgtggcaacg aatgctggcg cgcggctgga cgccggtcag tgaatcccgt
    37621 cttgacgact ggcttacgca agcgccagac ggcgtggtgt tattaagcag tgacccgaaa
    37681 cgcacgccag aggtcagcga taatccggta atgattggcg aattactgca cgagtttccc
    37741 gactatacat ggcaggtggc gattgctgac cttgagcaga gcgaagccat cggcgatcgc
    37801 tttggcgcct ttcgctttcc tgccacttta gtgtttaccg gcggaaacta tcgcggcgtg
    37861 ctgaatggta ttcacccgtg ggcggaactg ataaacctga tgcgcgggct tgtcgaaccg
    37921 cagcaggagc gtgcctcatg agcgaaactt ttttccatct gctggggcca ggaacgcaac
    37981 cgaacgatga cagtttcagc atgaatccac tgccgatcac ctgtcaggtg aatgatgaac
    38041 cgagtatggc ggccttggag caatgtgctc acagcccgca ggtgattgcg ctgttaaacg
    38101 agttacaaca tcaactaagc gaacgccagc cgccgttggg cgaggtgctg gcagtcgatc
    38161 tgttaaatct caacgccgac gatcgtcact ttatcaatac gcttctcggg gaaggggaag
    38221 tgtcagtgcg cattcagcag gctgacgaca gtaaaagtga aatacaggag gcgatcttct
    38281 gcggattatg gcgggtgcgc agacgtcgcg gcgaaaagtt gctggaggac aaactggagg
    38341 ctggctgcgc gccgctggcg ttgtggcagg cggcaacgca aaatctcttg ccgacagatt
    38401 cgctgttacc gccgcccatt gatggcctga tgaatggcct accgttggcg catgagttac
    38461 tggcacatgt acgtaacccc gacgcgcagc cgcacagcat taatctgacg caattaccca
    38521 tcagcgaggc tgatcggctt tttctctcac gtctctgtgg gccgggaaat attcagattc
    38581 gtaccattgg ctatggcgag agctatatca actccacggg gttacgccat gtctggcatt
    38641 tacgctgtac ggacacctta aaaggcccgt tactggaaag ttatgaaatc tgcccaatac
    38701 cggaagtggt gctggcagcg ccagaagatt tggtcgactc tgcgcagcgg cttagcgagg
    38761 tatgtcagtg gctggcggaa gctgcaccga cgtaaaaaga cggtaagtat cgctttcagt
    38821 cttatgaata tcgcaatcgg cgaatacctc tggtcgtaga gtttcaggat aaagagggag
    38881 atctaacatt atcgggttat ttttctctct tcgcctacag gagtgcgcat gtgggatgtc
    38941 attgatttat cgcgctggca gtttgctctg accgcgctgt atcacttttt atttgtaccc
    39001 cttaccctgg ggctgatttt tttgctggct atcatggaaa ccatttacgt ggtcaccggc
    39061 aaaacaatct accgcgatat gacgcgcttc tggggtaagc tcttcggtat caattttgct
    39121 cttggcgtgg ctaccggcct gaccatggag tttcagtttg gtactaactg gtcattctat
    39181 tccaactatg tgggcgatat ttttggcgca ccactggcga tggaagcatt aatggccttc
    39241 ttcctcgaat ccacctttgt cgggctgttc ttcttcggct ggcaacggct gaataaatac
    39301 cagcacctgc tggtgacgtg gctggtggcg ttcggttcaa atctctctgc attgtggata
    39361 ttgaatgcca acggttggat gcaatacccg accggtgcgc attttgatat cgacaccctg
    39421 cgtatggaga tgaccagctt cagcgagctg gtctttaacc cggtcagcca ggtgaaattt
    39481 gtgcacaccg tcatggcggg ctacgtgacc ggggccatgt ttattatggc gatcagcgcc
    39541 tggtatttac tgcgcggacg ggagcgcgat gtcgcattac gctcgtttgc catcggttcc
    39601 gtcttcggta ctctggcgat tatcggtacc ctgcaactcg gagacagttc tgcgtatgaa
    39661 gtcgcgcaag tacaaccggt aaaactggcg gcgatggaag gggagtggca aacggaacct
    39721 gctcctgcac cgttccatgt ggttgcctgg ccggaacagg atcaagagcg taacgccttt
    39781 gccatcaaaa ttcccgcgct gctagggatc ctcgccactc actcattaga taaacccgtg
    39841 ccgggtctga agaatttgat ggctgaaacc tacccacgct tgcaacgcgg acgtatggcc
    39901 tggctgttaa tgcaggaaat atctcaaggc aatcgtgagc cgcatgtgtt gcaggcattc
    39961 cgggagctgg aaggcgacct tggttacggc atgttgctct cccgctatgc gccggatatg
    40021 aatcatgtca cagccgcaca gtaccaggcg gcgatgcgtg gcgcgatacc tcaggttgcg
    40081 ccggtattct ggagtttccg catcatggtg ggctgtggtt ccctgctgct actggtgatg
    40141 ctgattgcgc ttgtccagac gctgcgtggc aaaatcgacc agcatcgctg ggtgctgaaa
    40201 atggcgctct ggagtttgcc gttgccgtgg attgcgattg aagccgggtg gtttatgacc
    40261 gagtttggtc gtcagccgtg ggcgatacag gacatcttac cgacatactc cgcgcactcc
    40321 gctttaacca caggacaact ggctttctca ctgatcatga tcgtaggtct ttacaccctg
    40381 ttcttaatcg ccgaagtcta cctgatgcag aaatatgccc gtctggggcc gagcgcgatg
    40441 cagagtgaac aaccgacgca gcaacagggg taaaggagaa aatcatgttt gattatgaaa
    40501 cattgcgctt catctggtgg ctgctgattg gcgtgatcct ggtggtcttt atgatctccg
    40561 acggatttga catggggatc ggctgtttgc tgccgctggt ggcgcgtaat gatgatgaac
    40621 gccggatagt gattaatagc gttggtgcgc actgggaagg caatcaggtc tggttgatcc
    40681 tcgcaggtgg ggcattattt gccgcctggc ccagagtgta tgcagcggcg ttttccggct
    40741 tttatgtggc gatgatcctg gtgctgtgct cgctgttctt ccgtccgctg gcctttgatt
    40801 atcgcggaaa aattgccgat gcacgctggc gtaaaatgtg ggatgccggt ctggtcatcg
    40861 gcagtctggt gccgccggta gtcttcggta tcgccttcgg caacttgttg ctcggcgtgc
    40921 cgtttgcctt cacaccgcaa ttacgcgtgg agtatctcgg cagcttctgg caactgctga
    40981 cgccattccc tttattgtgt ggtttgctca gccttgggat ggtgattttg caaggtggcg
    41041 tctggttaca actgaaaact gttggtgtga ttcatctgcg ttcacagctg gcgactaaac
    41101 gcgctgcact gttggtgatg ctgtgctttt tgctggcagg ttactggctg tgggtcggta
    41161 ttgatggctt tgtactgctc gcccaggatg ctaacggtcc ttccaatccg ttaatgaaac
    41221 tggtggcagt gctacctggt gcctggatga ataatttcgt cgaatcgccc gttttgtgga
    41281 tcttcccgct gctggggttc ttctgcccat tgctgacggt gatggcgatt tatcgtggtc
    41341 gcccgggttg gggatttttg atggcatcat tgatgcaatt tggcgtgatt ttcacggcag
    41401 gcatcacgct gttccccttt gtcatgccgt caagtgtgag tccaatctcc agcctgacgt
    41461 tgtgggacag cacttccagt cagctgacgc tgagcattat gttggtaatc gtgctgatat
    41521 ttttgcccat tgtgttgctc tacactctct ggagctacta caaaatgtgg ggacgcatga
    41581 caacagaaac tctccgccgt aacgaaaacg agttgtacta aggagcagga acatgtggta
    41641 tttactttgg ttcgtcggca ttttgttgat gtgttcgctc tccacccttg tgttggtatg
    41701 gctggacctg cgtctgaaaa gttaacgaac gtaggcctga tgcggcgcat tagcatcgca
    41761 tcaggcaatc aataatgtta gatatgaaaa gcggaaacat atcgatgaaa gcgatcttaa
    41821 tcccattttt atctcttctg attccgttaa ccccgcaatc tgcattcgct cagagtgagc
    41881 cggagctgaa gctggaaagt atggtgattg tcagtcgtca tggtgtgcgt gctccaacca
    41941 aggccacgca actgatgcag gatgtcaccc cagacgcatg gccaacttgg ccggtaaaac
    42001 tgggttggct gacaccgcgc ggtggtgagc taatcgccta tctcggacat taccaacgcc
    42061 agcgtctggt agccgacgga ttgctggcga aaaagggctg cccgcagtct gctcaggtcg
    42121 cgattattgc tgatgtcgac gagcgtaccc gtaaaacagg cgaagccttc gccgccgggc
    42181 tggcacctga ctgtgcaata accgtacata cccaggcaga tacgtccagt cccgatccgt
    42241 tatttaatcc tctaaaaact ggcgtttgcc aactggataa cgcgaacgtg actgacgcga
    42301 tcctttgcag ggcaggaggg tcaattgctg actttaccgg gcatcggcaa acggcgtttc
    42361 gcgaactgga acgggtgctt aatttcccgc aatcaaactt gtgccttaac cgtgagaaac
    42421 aggacgaaag ctgttcatta acgcaggcat taccatcgga actcaaggtg agcgccgaca
    42481 atgtctcatt aaccggtgcg gtaagcctcg catcaatgct gacggagata tttctcctgc
    42541 aacaggcaca aggaatgccg gagccggggt ggggaaggat caccgattca caccagtgga
    42601 acaccttgct aagtttgcat aacgcgcaat tttatttact acaacgcacg ccggaggttg
    42661 cccgcagtcg cgccaccccg ttattggatt tgatcatggc agcgttgacg ccccatccac
    42721 cgcaaaaaca ggcgtatggt gtgacattac ccacttcagt gctgtttatt gccggacacg
    42781 atactaatct ggcaaatctc ggcggcgcac tggagctcaa ctggacgctt ccaggtcagc
    42841 cggataacac gccgccaggt ggtgaactgg tgtttgaacg ctggcgtcgg ctaagcgata
    42901 acagccagtg gattcaggtt tcgctggtct tccagacttt acagcagatg cgtgataaaa
    42961 cgccgctgtc attaaatacg ccgcccggag aggtgaaact gaccctggca ggatgtgaag
    43021 agcgaaatgc gcagggcatg tgttcgttgg caggttttac gcaaatcgtg aatgaagcac
    43081 gcataccggc gtgcagtttg taatgcataa aaaagagcat tcagttacct gaatgctctg
    43141 aggctgatga caaacgaaga actgtctaat g
//
LOCUS       DAJTNM010000018        41031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTNM010000018 DAJTNM010000000
VERSION     DAJTNM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.018"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..221)
                     /locus_tag="OO172_001128"
     CDS             complement(<1..221)
                     /locus_tag="OO172_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HCR5511387.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIG"
     gene            complement(261..1106)
                     /gene="glpF"
                     /locus_tag="OO172_001129"
                     /pseudo
     CDS             complement(261..1106)
                     /gene="glpF"
                     /locus_tag="OO172_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            1532..1777
                     /gene="zapB"
                     /locus_tag="OO172_001130"
     CDS             1532..1777
                     /gene="zapB"
                     /locus_tag="OO172_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5511388.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(1862..2347)
                     /gene="rraA"
                     /locus_tag="OO172_001131"
     CDS             complement(1862..2347)
                     /gene="rraA"
                     /locus_tag="OO172_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5511389.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(2440..3366)
                     /gene="menA"
                     /locus_tag="OO172_001132"
     CDS             complement(2440..3366)
                     /gene="menA"
                     /locus_tag="OO172_001132"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5511390.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(3433..4764)
                     /gene="hslU"
                     /locus_tag="OO172_001133"
     CDS             complement(3433..4764)
                     /gene="hslU"
                     /locus_tag="OO172_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5511391.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(4774..5304)
                     /gene="hslV"
                     /locus_tag="OO172_001134"
     CDS             complement(4774..5304)
                     /gene="hslV"
                     /locus_tag="OO172_001134"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5511392.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(5397..6356)
                     /gene="ftsN"
                     /locus_tag="OO172_001135"
     CDS             complement(5397..6356)
                     /gene="ftsN"
                     /locus_tag="OO172_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5511393.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(6448..7473)
                     /gene="cytR"
                     /locus_tag="OO172_001136"
     CDS             complement(6448..7473)
                     /gene="cytR"
                     /locus_tag="OO172_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5511394.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(7629..9827)
                     /gene="priA"
                     /locus_tag="OO172_001137"
     CDS             complement(7629..9827)
                     /gene="priA"
                     /locus_tag="OO172_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5511395.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            10030..10242
                     /gene="rpmE"
                     /locus_tag="OO172_001138"
     CDS             10030..10242
                     /gene="rpmE"
                     /locus_tag="OO172_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5511396.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(10303..10911)
                     /locus_tag="OO172_001139"
     CDS             complement(10303..10911)
                     /locus_tag="OO172_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5511397.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(11095..11412)
                     /gene="metJ"
                     /locus_tag="OO172_001140"
     CDS             complement(11095..11412)
                     /gene="metJ"
                     /locus_tag="OO172_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5511398.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            11689..12849
                     /gene="metB"
                     /locus_tag="OO172_001141"
     CDS             11689..12849
                     /gene="metB"
                     /locus_tag="OO172_001141"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5511399.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            12852..15284
                     /gene="metL"
                     /locus_tag="OO172_001142"
     CDS             12852..15284
                     /gene="metL"
                     /locus_tag="OO172_001142"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5511400.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            15633..16523
                     /gene="metF"
                     /locus_tag="OO172_001143"
     CDS             15633..16523
                     /gene="metF"
                     /locus_tag="OO172_001143"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5511401.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            16852..19032
                     /gene="katG"
                     /locus_tag="OO172_001144"
     CDS             16852..19032
                     /gene="katG"
                     /locus_tag="OO172_001144"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5511402.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALLVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            19126..20031
                     /gene="yijE"
                     /locus_tag="OO172_001145"
     CDS             19126..20031
                     /gene="yijE"
                     /locus_tag="OO172_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5511403.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(20058..20675)
                     /locus_tag="OO172_001146"
     CDS             complement(20058..20675)
                     /locus_tag="OO172_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR5511404.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20950..22042)
                     /gene="gldA"
                     /locus_tag="OO172_001147"
                     /pseudo
     CDS             complement(20950..22042)
                     /gene="gldA"
                     /locus_tag="OO172_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(22053..22715)
                     /gene="fsa"
                     /locus_tag="OO172_001148"
     CDS             complement(22053..22715)
                     /gene="fsa"
                     /locus_tag="OO172_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5511405.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPCQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(22727..25228)
                     /gene="ptsP"
                     /locus_tag="OO172_001149"
     CDS             complement(22727..25228)
                     /gene="ptsP"
                     /locus_tag="OO172_001149"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5511406.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRVRTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            25537..26616
                     /locus_tag="OO172_001150"
     CDS             25537..26616
                     /locus_tag="OO172_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5511407.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            26631..26951
                     /locus_tag="OO172_001151"
     CDS             26631..26951
                     /locus_tag="OO172_001151"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5511408.1"
                     /translation="MTKIIAVTACPSGVAHTYMVAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            27002..29299
                     /locus_tag="OO172_001152"
     CDS             27002..29299
                     /locus_tag="OO172_001152"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5511409.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            29265..30143
                     /locus_tag="OO172_001153"
     CDS             29265..30143
                     /locus_tag="OO172_001153"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5511410.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            30145..30486
                     /locus_tag="OO172_001154"
     CDS             30145..30486
                     /locus_tag="OO172_001154"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5511411.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(30473..31324)
                     /locus_tag="OO172_001155"
     CDS             complement(30473..31324)
                     /locus_tag="OO172_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5511412.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(31473..33206)
                     /locus_tag="OO172_001156"
     CDS             complement(31473..33206)
                     /locus_tag="OO172_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5511413.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(33389..36040)
                     /gene="ppc"
                     /locus_tag="OO172_001157"
     CDS             complement(33389..36040)
                     /gene="ppc"
                     /locus_tag="OO172_001157"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5511414.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLDYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(36392..37543)
                     /gene="argE"
                     /locus_tag="OO172_001158"
     CDS             complement(36392..37543)
                     /gene="argE"
                     /locus_tag="OO172_001158"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5511415.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            37697..38701
                     /gene="argC"
                     /locus_tag="OO172_001159"
     CDS             37697..38701
                     /gene="argC"
                     /locus_tag="OO172_001159"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5511416.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            38709..39485
                     /gene="argB"
                     /locus_tag="OO172_001160"
     CDS             38709..39485
                     /gene="argB"
                     /locus_tag="OO172_001160"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5511417.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            39546..40919
                     /gene="argH"
                     /locus_tag="OO172_001161"
     CDS             39546..40919
                     /gene="argH"
                     /locus_tag="OO172_001161"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5511418.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         9342 a        10531 c        11258 g         9900 t
ORIGIN      
        1 ataccgatag ctgcaatttg atcggaactg atatcggctt tcgccagcac ttctaccagc
       61 gtggagcttt gggtggccca gatttccatt gggtcgtgtt ctacccaacc tggttttggg
      121 tagatttgct caaattcgcg ctgcgacacg ctaatgatat tggcatcgtg atccattacg
      181 accgcgcggg agctggtggt gccctggtcg agcgcaacga tatatttttt tcagtcatgt
      241 ttaattgtcc cgtagtcata ttacagcgaa gctttttgtt ctgaaggagt tgtggtttcc
      301 ttttcttcca caacacagat atcgcaaggc aaatggcgac caatcagttt gcggtaggca
      361 aatgcaccta caatcgcgcc aacgataggg ccgaaaagcg gcaccaggaa gtaaggaatg
      421 tctctgccgc cggtaaaggc gacattgccc cagcccgcca gctaggcaaa gactttcgga
      481 ccgaagtcac gcgctgggtt catggcaaaa cctgtcaatg ggcccataga tgcgccaatg
      541 accgcaatca gtagaccaat cagcaaggga gccaaagggc cgcgtggtac accgttgcca
      601 tcgtccgtta acgccaggat aagccccatc agaatagcgg taatcaccat ctcaactgcg
      661 aaagcctgca caaaattgat atgaggatta gggtaagtag agaaagtgcc agccagatca
      721 acactttcaa cgctgccgcg aacaatgtga tgagtctgct cgaagtcgaa aaataaattg
      781 tagtaaagcc cgtaaactaa agccgcagcg cagaaagcgc cggcaacttg tgaaacgata
      841 aaaggaataa ctttgcgctt gtcgaaacag gcaaacagcc acaatgcaat ggtaacagcg
      901 ggattaagat gcgcgccgga aacccctgcg gtcaggtaga tggccattgc cacccccagt
      961 ccccaaatga cactgatttc ccactgacca aaagacgcac cagcgacttt tagtgctgca
     1021 acgcaaccca caccgaaaaa aatcaacaac ccggtaccga ggaattcagc aatgcactgg
     1081 cctttcaagg ttgatgtttg actcataatc ggatcctgaa gagttaatgt ttgttgtatg
     1141 tgtgaaagtc acggacctcc acgatgcttg taggcatgct gttaacttat cgttaacgag
     1201 caaaaacgag aaatatcgaa cttaaaatgt gtgtgcttcg tcataaaatg agcgttatcg
     1261 cgccatttat tccatctgat tgtacatgat gcatgtttgt tagatcattc gcgcaacaaa
     1321 tttattaaca ttttcgattg atgtggatta tgtcactagg agaaggatga aaagtgtggc
     1381 aaaccgtaat ctgcaaggcg tgccgctgga cagcgatggc gcggcttcat acaatcggag
     1441 ctaactaaag tgcgctcgta tttattaagg cgtcaccggt aatcgggacg aggatttttt
     1501 atccatcaac gccttgcaat tcaggagagg tatgacaatg tcattagaag tgtttgagaa
     1561 actggaagca aaagtacagc aggcgattga taccatcact ctgttgcaga tggaaatcga
     1621 agagctgaaa gaaaaaaaca actcactgtc gcaggaagtt caaaatgccc agcatcagcg
     1681 cgaagagctg gagcgtgaga acaaccatct gaaagaacag cagaacggct ggcaggaacg
     1741 tctgcaggcc ctgctgggtc gcatggaaga ggtctgatta cgcttcatct ttcgcgtaat
     1801 ttctttactg tgtaggccaa caggtaacgc agaaaaaagg caccttgcgg tgcctttctt
     1861 atcattcaat atccagcgga tcttctgaaa gaataatccc ggtattgtcg gcataaagat
     1921 ggtcgccgga gaagaaggtg acaccgccaa aattgacgcg gacatcgctt tcgccaatgc
     1981 cttcgccagc ggcaccaacc ggaattgccg ccatcgcctg gatgccgata tccaactctt
     2041 ccaggtcatc tacctgacgc accgcgccgt aaatgaccag accttcccat tcattttgta
     2101 ctgccagacg cgccagttca gcatcgacca gtgcgcgacg aacagaacca ccgccatcga
     2161 cgacaagaac acgaccacgg ccattctgtt cgagcagatc gtacagcaac ccgttgtcct
     2221 cgaaacattt taccgtgatt atttgtccgc caaacgacgc ccgtccgcca aagttggaga
     2281 acagcggttc cacgacgtta acatcttctt gatagatgtc acaaagctcg gaagtatcgt
     2341 atttcatagg cttaacattc agttgctgcg agaattttca gtatatcgcg ctatgtgggc
     2401 tgttggcaaa gtcatcaatt gttaattgat atttgtcagt tatgctgccc actggcttag
     2461 gaatatccct aaaacaaaca gcaggttagt cagtaacgct cccttgacag tacgttccag
     2521 cattggtcgc atcgccaccg ggtccatttc ccgcatcaca taacgggctt gcttcaccag
     2581 taatggtgcc gccagcagga acagccagcc ccacaggcta tgcagcgaaa agagattaaa
     2641 cagcgccaga cacaccagcg agcccatcag caggcaggca tgataacgac gcgcgttcac
     2701 ttcacctaag cgcaccacca gcgtgttttt gccattttcg cggtcgctat tgatatcacg
     2761 caggttatta atattcagta ctgccgttgc cagcaggccg catgcggtcg ccggaaggat
     2821 cagtgccgga atcaatgtat gagcctgtaa ataccagctc cccatgacac tcaaccagcc
     2881 aaagaaaacc agtacggaaa tatcacccag accgatataa ccataaggac gattgcccac
     2941 ggtgtaggtg atagcggcaa tgatcgacaa cccgccaaga atcaggaaac cgacaaaatc
     3001 ggccagcgta tggcatgcca ctgcaaccag tgccagcccg gagagacaga tgagcacgac
     3061 ggtaataatg agcgcccgtt tcatcttttg ctgggtaatg accccttttt gcatgccgcg
     3121 tagcggccca atgcggtcag gtttatcgct gccttttacc gcatcgccgt aatcattggc
     3181 gaggttagaa aggatctgta atagcccggc ggtaattagt gccagcaggg cgaccagcgg
     3241 atcgaagtga ccttgccacc atgccagcgc cgtcccgacg ataattgcag caaaggcgag
     3301 ggggagggtt ttaggtcgta aactttccag ccacgcctga gttcggctaa tttgttgttc
     3361 agtcataata cgcgccaata aaaatggggc ctttcagccc catcaaacaa tgatgaaaat
     3421 gattgaacgc gattatagga taaaacggct cagatcttca tctgccacca acgcatccag
     3481 atgtttgctc acataatctg cgtcaatagt gatattttga ccgcttaaat cgctggcgtc
     3541 gtaggaaatc tcttccatta aacgctccag aacagtgtgt aaacgacgag caccgatgtt
     3601 ttcggtagat tcgttcacct gccatgccgc ttccgcgatg cgtttaatac cggagtcggt
     3661 aaactcgata tttacgcctt cagtcgccat cagtgctttg tactgcacgg tgatagaggc
     3721 attcggctcg gtcagaatac gctcgaagtc gctggtggtc agtgcctgca gttcaacgcg
     3781 gattggcagc cgaccttgca gttccgggat caggtcagac ggtttcgcaa tctggaacgc
     3841 gccagaagcg ataaacagaa tgtggtcagt tttgaccatc ccgtgtttgg tggaaacggt
     3901 gcaaccttct accagcggca gcaggtcacg ctgaacgcct tcacgagaaa catccggacc
     3961 ggaagactcg ccgcgcttac agattttgtc gatttcgtcg ataaacacga tcccgtgctg
     4021 ctcaacagcg tcgatagcgt cttgcttcag ctcttccggg ttcaccagtt tcgccgcttc
     4081 ttcttcaatc agcagcttca tggcgtcttt gattttcagc ttacgcgctt tttgcttctg
     4141 gccgcccagg ttctggaaca tggactgcag ctggctggtc atctcttcca tgcccggagg
     4201 agccataatt tcaacgccca tcggtgctgc ggcaagatcg atctcgattt ctttgtcatc
     4261 aagctggcct tcacgcagtt ttttgcggaa tgcctgacga gcagcggaag gttcctgctg
     4321 ctgttcagtc tgtccccagt tgtttttagc aggtgggatc agcacgtcga gaatacgttc
     4381 ttctgccagt tcttcagcgc gataacggtt tttctcgata gcctggacgc gtaccatttt
     4441 cacggcggca tcggtcagat cgcgaataat agaatcaact tccttaccga cgtagcccac
     4501 ttcggtaaat ttggtcgctt caactttgat gaacggcgca ttcgccagct tagccagacg
     4561 acgggcgatt tcagttttac cgacaccggt cgggccgatc atcaggatat ttttcggggt
     4621 cacttcatgg cgcagctctt cgttgagctg catgcgacgc cagcggttac gcagagcaat
     4681 cgccacagaa cgcttggcgt tgtcctggcc gatgatgtgc ttatccagtt cgctgacgat
     4741 ttcgcgtggg gtcatttcag acatgggaga tccttacgct ttgtagctta attcttcgat
     4801 ggtgtggaaa tggttggtat agatgcaaat gtcgcctgca atatccaacg ccttttcagc
     4861 aatttcacgg gcgctaagtt cagtgttttc taacagcgcg cgcgccgcag cctgggcgta
     4921 agggccgccg gagccgatag caataagatc gttttctggc tgcaccacgt caccgttacc
     4981 ggtgatgata agcgatgcag tttcatccgc gactgccagc agtgcttcaa gtttgcgcag
     5041 catgcgatcg gtacgccagt cttttgccag ctcaacggcg gctttaacca gatggccctg
     5101 atgcatttcc agtttacgtt caaacagttc gaacagcgta aaagcatccg cagtaccgcc
     5161 cgcaaagccc gcgatgactt tgtcgttgta cagacggcgg acctttttca cgttgccttt
     5221 cattacggta ttgcccaacg tggcctgacc atcaccagcg atgaccacat ggccgttacg
     5281 gcgtacgctt actatagttg tcacgagctg accccttggt tacgaataca gagtacaaac
     5341 cccgtacaaa agtacggggc ataatgcaat tatagatggg ggggattttg agggtttcaa
     5401 cccccggcgg cgagccgaat gcagtttgta tgacccgcca tcttcaaccg attgagggtg
     5461 ctgtctgcgt tctctttgcc tttcaccggg ccaatgacca cacgattcca gccattgttg
     5521 gtggtgattt tcgagtcaaa tccttcgaac gccagctgag cacgtaccgt ctctgcctgt
     5581 tccgcgcctc tgaacgaacc gcactgcacc atccagcggc gttcgtcttt tttctccgcc
     5641 gtcggttttg gcgcgtcagc ggcacgagca accggcgcag cttgctgcgg cttcgactgc
     5701 gcggtcgtgt gcgcaggggt ttgcagcaga tcctggtacg gctgctgagt agaagctggt
     5761 ttagactggc gcggttgcgc ctgtaccggc gcggcttgcg acgtacgcgt ctgctgctgc
     5821 cagctttgtt cagtcgtacg cgactgctga gccaggcgtt gctgctccgc taattgctgc
     5881 gcctgacgct ggcgttgtag cgtttgctga cgctgttccg gcgtctgttc attccacgga
     5941 acctcaacca gctgcgttgg ctgctggcgc atatcagcct gcatttgttc aagaagctga
     6001 cgttgttctg gtgtcagttg ctccggcgtt ttcacttcac caccggcaga aggttctgtg
     6061 ggcgcacgca cacccggctg gcgactttcc agctctttaa tgtagcgcca gcgttcttct
     6121 ggttttggtg gtagtccgtt tccggtcact ttctggcttt gcagcgtctc ggactcttct
     6181 ttcttgtgat gcgtaatgaa gtacagacca ccgataaagg tcacaagaac ggcggcagca
     6241 atagcgacca tagcgggaga aaccgcaggc agatttcgtt gctttttccg tgaggtgctc
     6301 ttttttcgcc gcgaaggtgc cggttggctg cggcgtacat aatctcgttg tgccactatc
     6361 gtttcgctgt atttattcgt tcgtcagccc gccatgttac ttaagcggcg ggcctttgac
     6421 cagtcacggc agtcttaaag gtttacttta aggtaacgcg cgtgttgatc cccggatgat
     6481 aagttcgcag tccattaaac gagagccact gccaacgtgt tgcccctgca tttgatcaag
     6541 caataacagc atagcttccc gaccgatttc gtaacgcggc tgcgcgatgg ttgtcagcgg
     6601 cggatcacaa aattgcgtca ggtcgatgtt atcaaaaccg attatggaaa ggtcttccgg
     6661 gactttcagc ccctggcgtt ttgcctgaga aagtgcgccg agcgccatca catcgctatg
     6721 gcagaagact gcagtaggcg gttgtggaag atcaagcagc tgttgcattg ctttgcttcc
     6781 ggcttcgaag gtgaagtcgc cacgggcgat gtattgtgga tcaaccataa ttccgcagcg
     6841 acgcagcgcc tgaacatagc cttgcaggcg gtagtgacac agtggcatct cttcgggacc
     6901 ggctatacag ccaatccgtt tatgcccttg ctcatataaa taatttactg catcaaatgc
     6961 ggcggtcaga ttgtcgatat gaacggtagg cagctccagt tccggtgcaa attcgttcgc
     7021 catcaccatc ggcggcagat tacgctgttc ctcaatgctg gcatcaaacg gcagccttga
     7081 acccagcagc aacatgccat caatttgctt ggtgatgatc aaatcgataa aggttttttc
     7141 ctgctgattt tgatgcgcac agtcgccaat cagcaccaga tatccgtgat ttgccgccgt
     7201 aacttcgata ccgcgaataa tttcgctaaa gaagggatcg cagatatccg ggacaatcac
     7261 cagaatggtg cgggattcat tacgcttgac gttgcgcccc ataggctgcg gtaaataacc
     7321 cacttcccgg gccgcttttt caacccgatt acgggtggcc tgggagactt tatcgggatt
     7381 cattaatgct cgggagacag tcgctgtaga gacttttgcc ttgagggcaa cgtctttcat
     7441 ggtcgcggca gtttcctgct tcttcgcttt cacactcact cctcgcctgg cacgtcaggc
     7501 gtactacatc catgtttact tcacatcggc aacattttta gcagatagcg cgtgaaaacg
     7561 gttacagaat tttcatgaaa agtgtgatga atattgaatt tttcgatccg cctcgcatcg
     7621 taagcggttt aaccctcaat cggatcaaca tccagtaccc atttcacctt gcgggaatcc
     7681 ggtattgtat tgatgagcgc cagcgtacca ctaatgatgt gttgcaagcg cacgcgggaa
     7741 gggtgctgca acaatatctg ccagcgccag cggccgccac gtttaggtgc cagtgccgga
     7801 accggaccga gaacccacag tttgtcgtct gccagcggac tggagaggat cagattacgc
     7861 agttgttgca ggaacagcgg cgcatgctga ttgttatgat cttccgcacg cacaatcaca
     7921 tggctggtcc atggcggcag ttgcatcatc cgtcgctcag ccagcgcctg ttcggcaaag
     7981 gcgtcgtagc ctttatagag caacgtttgc aacagaggat gttccggatg gtgcgtttgc
     8041 agcaccactt cgccttgttt acccgcacgc ccggcacgac cagcgacctg ggtgtaaagc
     8101 tgagcgaaac gctctgccga gcgaaaatcg gcagaaaaca gcgcgccgtc cacatccagt
     8161 aacgccacca gcgtcacatc cggaaagtgg tgacctttcg ccagcatttg tgtgccgatc
     8221 aaaatcctcg caccgccgcg atgtacttcc gccagttgct gttccagcgc ccctttgcgg
     8281 ctggtggtat cgcggtcgat acgagaaatg ggcacatcag ggaacagcgg cgcaagcgtc
     8341 tgttcaagct gttcggtgcc cagccccacg ggtaccaggt gcgtggaacc gcaggaaggg
     8401 cactggcgcg gcaccggacg ctgactgtca cagtggtgac agcgcaaatg ctgttgcgcc
     8461 tgatgcagcg tgtagtagtg atcgcaacgt gggcattcgg caatccagcc gcagtcgtgg
     8521 cacagtagcg caggcgcaaa gccacggcgg ttaagaaaga ggataacctg attgttggcc
     8581 tgtaaatgct ggcgcatacg ggtgattaac gccggagcca gacctgcctg caccttctga
     8641 ccttttaaat ccagcacgtg ttgaattgcc ggacgcgcat tccccgcccg gcgcgtcagg
     8701 cgcagcaggc ggtatttttt ctgctggacg ttgcacaacg tttccagcgc gggcgttgcg
     8761 gagccaagaa taatcgggat ttgctcgcta tgcgcacgat acaccgccag gtcgcgggca
     8821 tgatagcgcc agccttcctg ctgcttgtag gaactgtcgt gctcttcgtc aatgacaatc
     8881 acgccgagat ttttaaacgg cgtaaacagc gcggagcggg tgccgatcac aatcgccgcc
     8941 tcgccatttt tcgctttcag ccacgccgaa agacgctcgc tgtcgttcag accggaatgc
     9001 agaacttcca cgggcgcatt aaaccgttca cgaaaacggg cgatagtttg cggtgtcagg
     9061 ccgatttccg gcaccatcac cagcgcctgt ttgccctgag cgagcacgtt ttccagtaca
     9121 ctgagataaa cctccgtttt accggaaccg gtaacgcccg ccagcagcca ggcagaaaaa
     9181 gtatctgccg cgctatgaat tgcgccaacg gcggtggcct gttcggtatt caatcgcaac
     9241 cgctcaccag aaacggcata gttcgttcgc cagtcgctaa actctggtgt ttcacttgct
     9301 aaatcacaca gacctttttt gcgcagcgcc tgcaacgcgg catcattaaa ttcgagcgtg
     9361 gcaacctggt cgcgccagat tttgccttgc cgtaacgctg ccagcgcctg ttgttgcttg
     9421 ggtgagcgtt tcaggctgtt cagatccacc gcctgacctt gttcagtggc aaaccagtac
     9481 cacatcggcg cgttcgccgc aggccgcccc tggcgtagta aaatcggcaa ggcatgaaac
     9541 agcacatcgc caatcggatg atgatagtaa tctgccgccc atagcagcaa tcgccagacg
     9601 gagtgagtaa acaccggctc accatccagc acttcgacta ccgcttttag ctcagtgagc
     9661 ggcagttcgc tggcatcgct aactgatacc acaatcccga tgcgctcctg ctgtttgcca
     9721 aacggcacgc gcacgcgaca cccagcttta accgtcatgc cttctggcag cagatagtca
     9781 aaggtacgag gaagcggaac gggcaaggca acgtgggcaa cgggcatagc atcatcctga
     9841 cttgaaattc ggtgggttag tatacacatt gccgtagaag agtgcggatc agtttgcata
     9901 cgctggttaa tttctgtatg atttcgcgcc ttcgtacgaa atgatcgtat tgaagctata
     9961 cttttaacat cgcgtggtgt ctggcgttag ggctggaaga gcgacgcggc cttaaaccga
    10021 ggttttccca tgaaaaaaga tattcacccg aaatacgaag aaattactgc tagctgctct
    10081 tgcggtaacg taatgaaaat ccgctccacc gttggtcatg acctgaacct cgacgtgtgc
    10141 agcaagtgcc acccgttctt cactggcaaa cagcgtgatg ttgctaccgg tggccgtgtt
    10201 gaccgcttca acaagcgttt caacatcccg ggcagcaaat aagaccggat ttccgaaaaa
    10261 agcgccgcac ggcgcttttt ttgtgcctga attttggtcg tatcaccacg aaaacagcgg
    10321 ccactctttg gcaaccgtgg tgagttgtgg cgcgtcgaaa acggcttgtg gtgaaactac
    10381 cgtttcctca agcggaatat ttttaccgta gcgttctttg agcttcgcct ggaccagcgg
    10441 gttgctgaga tcaaactctt tcaacttctg ctgttcgccc acgcgcattc ccagcgcgtt
    10501 ctggtaaacc ttccacacca cttccgaaca gtactggcga tcgtcgctcc atgagaagct
    10561 gaaatcatac ggtttaccga gataacgttt tgccgtttgc gtcagttttt gctgttgttc
    10621 aacactcagt ccgccttcaa cgcggcgaac aacgtagttg cccttttcac catgggcaat
    10681 ccactgcttg agcggggtgt atttcaccgg gccgaccgct tcaaaaacgt agggcttttt
    10741 gttacgcatc accagcatac cggtgtggct gtaatcggaa tgggttgcca gttggatcgc
    10801 tttactttgc gatgagcgag agatttgaaa gatgatgtcg ccagtttgcg gctgccaggc
    10861 aaaggcaggt acagaaacca gcaggctgag gatcagcagc ctgtttttca tagttaaacg
    10921 tccatgtata aaaagcggtg ggtcgcagac aacgtgctcg ttgtttatgc cggatgcggc
    10981 gtgaacgcct tatccggcct acaagttcgt gcaaattcaa taaattgcaa tatgacgtag
    11041 gcctgataag cgtagtgcat caggcgattc cactccgcgc cgctcttttc cgctttagta
    11101 ttcccacgtc tccgggttaa tccccatctc acgcatgatc tcttttgccg cttccgggat
    11161 ttcgtcgctg cgctctttac gcagatcggc atcatccggc aaaggttgcc cggtaaaggc
    11221 atgcagaaac gcttcgcaca gcagctcgct gttggtagcg tgacgcaggt tgttcacctg
    11281 acgacgcgtg cgttcatcgg tgaggatttt taacacctta agaggaatgg aaaccgtaat
    11341 ctttttgact tgttcactct tcttgccgtg ttcagcgtat gggctgatat attcgccgct
    11401 ccattcagcc atgagatact taatcctctt cgtcaataaa ttgagaccag accccacaat
    11461 ttatgtgggt actgaccgta aacccgcata gtttaccgta caggcgttac cgtgacatcg
    11521 tgtaatgcac ctgtcggcgt gataatgcat ataattttaa cggctatttg ggatttgctc
    11581 aatctatacg caaagaagtt tagatgtcca gatgtattga cgtccattaa cacaatgttt
    11641 actctggtgc ctgacatttc accgacaaag cccaggaaac ttcatcacat gacgcgtaaa
    11701 caggccacca tcgcagtgcg tagcgggtta aatgacgacg aacagtatgg ttgcgttgtc
    11761 cctcccatcc atctttccag cacctataac tttaccggat ttaatgaacc gcgcgcgcat
    11821 gattactcgc gtcgcggcaa cccaacgcgc gatgtggttc agcgtgcgct ggcagaactg
    11881 gaaggcggtg ctggcgcagt acttactaat accggcatgt ccgcgattca cctggtaacg
    11941 accgtctttt tgaaacctgg cgatctgctg gttgcgccgc acgactgcta cggcggtagc
    12001 tatcgcctgt tcgacagtct ggcgaaacgc ggttgctatc gcgtgttgtt tgttgatcaa
    12061 ggcgatgaac aggcattacg ggcagcgctg gcagaaaaac ccaaactggt actggtagaa
    12121 agcccaagta atccattgtt acgcgtcgtg gatattgcga aaatctgcca tctggcaagg
    12181 gaagtcgggg cggtgagcgt ggtggataac accttcttaa gcccggcatt acaaaatccg
    12241 ctggcattgg gtgccgatct ggtgttgcat tcatgtacga aatatctgaa cggtcactct
    12301 gacgtggtgg ctggcgtggt gattgctaaa gacccggacg ttgtcactga acttgcctgg
    12361 tgggcaaaca atattggcgt gacgggcggc gcgtttgaca gctatctgct gctacgcggg
    12421 ttgcgaacgc tggtgccgcg tatggagctg gcgcagcgca acgcgcaggc gattgtgaaa
    12481 tatctgcaaa cccagccgtt ggtgaaaaaa ctgtatcacc cgtcgctgcc ggaaaatcag
    12541 gggcatgaaa ttgccgcgcg tcagcaaaaa ggctttggcg caatgttgag ttttgaactg
    12601 gatggcgatg agcagacgct gtgtcgtttc ctgggcgggc tgtcgttgtt tacgctggcg
    12661 gaatcattag ggggagtgga aagtttaatc tctcacgccg caaccatgac acatgcaggc
    12721 atggcaccag aagcgcgtgc tgccgccggg atctccgaga cgctgctgcg tatctccacc
    12781 ggtattgaag atggcgaaga tttaattgcc gacctggaaa atggcttccg ggctgcaaac
    12841 aaggggtaaa aatgagtgtg attgcgcagg caggggcgaa aggtcgtcag ctgcataaat
    12901 ttggtggcag tagtctggct gatgtgaagt gttatttgcg tgtcgcgggc attatggcgg
    12961 agttctctca gcctgacgat atgatggtgg tttccgccgc cggtagcacc actaaccagt
    13021 tgattaactg gttgaaacta agccagaccg atcgtctctc tgcgcatcag gttcaacaaa
    13081 cgctgcgtcg ctatcagtgc gatctgatta gcggtctgct acccgctgaa gaagccgata
    13141 gcctcattag cgcttttgtc agcgatcttg agcgcctggc ggcgttgctc gacagtggta
    13201 ttaacgatgc ggtgtatgcg gaagtggtgg gccacggtga agtgtggtcg gcgcgcctga
    13261 tgtctgcggt acttaatcaa caagggctgc cagcggcctg gcttgatgcc cgtgagtttt
    13321 tacgcgctga acgcgctgcg caaccgcagg ttgatgaagg gctttcttac ccgttgctgc
    13381 aacagctact ggtacaacat ccgagcaaac gtctggtggt gaccgggttt atcagccgta
    13441 acaacgccgg tgaaacggtg ttgctggggc gtaacggttc cgactattcc gcgacacaaa
    13501 tcggtgcgct ggcgggtgtt tctcgtgtaa ccatctggag cgacgtcgcc ggggtataca
    13561 gtgccgaccc gcgtaaagtg aaagatgcct gcctgctgcc gttgctgcgt ctggatgagg
    13621 ccagcgaact ggcgcgtctg gcggctcccg ttcttcacgc ccgtacttta cagccagttt
    13681 ctggcagcga aatcgacctg cagctgcgct gtagctacac gccggatcaa ggttccacgc
    13741 gcattgaacg cgtgctggcc tccggtactg gtgcgcgtat tgtcaccagc cacgatgatg
    13801 tctgtttgat tgagtttcag gtgcccgcca gtcaggattt caaactggcg cataaagaga
    13861 tcgaccagat cctgaaacgt gcccaggtgc gtccactggc ggttggcgtg cataacgatc
    13921 gccagttgct gcaattttgc tacacctcag aagtggccga cagtgcgctg aaaatcctcg
    13981 acgaagcggg attacctgga gaactgcgcc tgcgtcaggg gctggcgctg gtggcgatgg
    14041 tcggggcggg tgtcacccgt aacccgctgc attgccaccg cttctggcag caactgaaag
    14101 gccagccggt cgagtttacg tggcagtccg atgacggcat cagtctggtg gcagtactgc
    14161 gcaccggccc gaccgaaagc ctgattcagg ggctgcatca gtccgtcttc cgcgcagaaa
    14221 aacgcatcgg cctggtattg ttcggcaaag gcaatatcgg ctctcgctgg ctggaactgt
    14281 tcgcccgcga gcagagcacg ctttcggcgc gtactggctt tgaatttgtg ctggcaggcg
    14341 tggtggacag ccgccgcagt ctgttgagtt atgacgggct ggacgccagc cgcgcgttag
    14401 ccttcttcaa cgatgaagcg gtcgagcagg atgaagagtc gcttttcctg tggatgcgcg
    14461 cccatccgta tgatgattta gtggtgttgg acgttaccgc cagccagcag cttgctgatc
    14521 agtatctcga tttcgccagc cacggtttcc acgtcatcag tgccaacaaa ctggcgggag
    14581 caagcgacag caataaatat cgccagatcc acgacgcctt cgaaaaaacc gggcgtcact
    14641 ggctatacaa cgccaccgtt ggtgcgggct tgccgatcaa ccataccgtg cgcgatctga
    14701 tcgacagcgg cgataccatt ttgtcgatca gtgggatctt ctccggcacg ctctcctggc
    14761 tgttcctgca attcgacggt agcgtgccgt ttaccgagct ggtggatcag gcgtggcagc
    14821 agggcttaac cgagcctgat ccgcgtgacg acctctccgg caaagacgta atgcgcaagc
    14881 tggtgattct ggcgcgtgaa gcgggttaca acattgaacc ggaccaggta cgtgtggaat
    14941 cgctggtgcc tgctcattgc gaaggtggca gcatcgacca tttctttgaa aatggcgatg
    15001 aacttaacga gcagatggtg caacggctgg aagcggcccg cgaaatgggg ctggtgctgc
    15061 gctacgtggc gcgtttcgat gccaacggca aagcgcgtgt gggcgtggag gcggtgcgtg
    15121 aagaccatcc gctggcatca ctgctgccat gcgataacgt ctttgccatc gaaagccgct
    15181 ggtaccgcga taatccgctg gtgatccgtg ggcctggcgc tgggcgcgac gtcaccgccg
    15241 gggcgattca gtcggatatc aaccggctgg cacagttgtt gtaatttcag aaatttaata
    15301 atgcccggta ctcatgtttt cgggtctatg gtttctaatg aaatatattg aattatcata
    15361 gaattaggcc ggatgaagcg tttacaacga atccggcaag aagcaataag tacatggtta
    15421 gtttatattt gcagtccggt ttgctttgca tatcggattt tctttttctt accatcctga
    15481 agttttttca tcttccctga tttttcctca ccatcattgg tcatttttcg gttgacgccc
    15541 ttcggctttt ccttcatctt tacatctgga cgtctaaatg gatagatgtg cacaacacaa
    15601 catataacta caagcgattg atgaggtaag gtatgagctt ttttcacgcc agccagcggg
    15661 atgccctgaa tcagagcctg gcagaagtcc aggggcagat taacgtttcg ttcgagtttt
    15721 tcccgccgcg taccagtgaa atggagcaga ccctgtggaa ctccatcgat cgccttagca
    15781 gcctgaaacc gaagtttgta tcggtgacct atggcgcgaa ctccggcgag cgcgaccgta
    15841 cgcacagcat tattaaaggc attaaagatc gcactggtct ggaagcggca ccgcatctta
    15901 cttgcattga tgcgacgccc gacgagctgc gcaccattgc acgcgactac tggaataacg
    15961 gtatttgtca tatcgtggca ctgcgtggcg atctgccgcc gggaagtggt aagccagaaa
    16021 tgtatgcttc tgacctggtg acgctgttaa aagaagtggc agatttcgat atatccgtgg
    16081 cggcgtatcc ggaagttcac ccggaagcaa aaagcgctca ggcggatttg cttaatctga
    16141 aacgcaaagt ggatgccgga gccaaccgcg cgattactca gttcttcttc gatgtcgaaa
    16201 gctacctgcg ttttcgtgac cgctgtgtat cggcgggcat tgatgtggaa atcatcccgg
    16261 gcattttgcc ggtatctaac tttaaacagg cgaagaaatt tgccgacatg accaacgtgc
    16321 gtattccggc gtggatggcg caaatgtttg acgggctgga tgacgatgcc gaaacccgca
    16381 aactggtggg cgcgaatatt gccatggata tggtgaagat tttaagccgt gaaggagtga
    16441 aagatttcca cttctatacg cttaaccgcg ctgaaatgag ttacgcgatt tgccatacgc
    16501 tgggtgttcg acctggttta taaatagtgt ggcttttgtg aaaatcacac agtgatcaca
    16561 aattttaaac agagcacaaa atgctgcctc gaaatgaggg cgggaaaata aggttgtcag
    16621 ccttgttttc tccctcatta cttgaaggat atgaagctaa aacccttttt tataaagcat
    16681 ttgtccgaat tcggacataa tcaaaaaagc ttaattacga tcaatttgat ctacatctct
    16741 ttaaccaaca atatgtaaga tctcaactat cgcatccgtg gattaattca attataactt
    16801 ctctctaacg ctgtgtatcg taacggtaac actgtagagg ggagcacatt gatgagcacg
    16861 tcagacgata tccataacac cacagccact ggcaaatgcc cgttccatca gggcggtcac
    16921 gaccagagcg cgggggcggg cacaaccact cgcgactggt ggccaaatca actccgtgtt
    16981 gacctgttaa accaacattc taatcgttct aacccgctgg gtgaggactt tgactaccgc
    17041 aaagaattca gcaaattaga ttactacggc ctgaaaaaag atctgaaagc cctgctgaca
    17101 gaatctcagc cgtggtggcc agccgactgg ggcagctacg ccggtctgtt tattcgtatg
    17161 gcttggcacg gcgcaggtac ttaccgttca atcgatggac gcggcggcgc gggtcgtggt
    17221 cagcaacgtt ttgcaccgct gaactcctgg ccggataacg taagcctcga taaagcgcgt
    17281 cgcctgttgt ggccaatcaa acagaaatat ggtcagaaaa tctcctgggc cgacctgttt
    17341 atcctcgcag gtaacgtggc gctggaaaac tccggcttcc gtaccttcgg ttttggtgcc
    17401 ggtcgtgaag acgtctggga accggatctg gatgttaact ggggtgatga aaaagcctgg
    17461 ctgactcacc gtcatccgga agcgctggcg aaagcaccgc tgggtgcaac cgagatgggc
    17521 ctgatttacg ttaacccgga aggcccggat cacagcggcg aaccgctttc tgcggcagca
    17581 gctatccgcg cgaccttcgg caacatgggc atgaacgacg aagaaaccgt ggcgctgatt
    17641 gcgggtggtc atacgctggg taaaacccac ggtgccggtc cgacatcaaa cgtaggtcct
    17701 gatccagaag ctgcaccgat tgaagaacaa ggtttaggct gggcgagcac ttacggcagc
    17761 ggcgttggcg cagatgccat tacctctggt ctggaagtag tctggaccca gacgccgacc
    17821 cagtggagca actatttctt cgagaatctg ttcaagtatg agtgggtaca gacccgcagt
    17881 ccggctggag caatccagtt cgaagcggta gacgcaccgg aaattatccc ggatccgttc
    17941 gatccgtcga agaaacgtaa accgacaatg ctggtgaccg acctgacgct gcgttttgat
    18001 cctgagttcg agaagatctc tcgtcgtttc ctcaacgatc cgcaggcgtt caacgaagcc
    18061 tttgcccgtg cctggttcaa actgacgcac agggatatgg ggccgaaatc tcgctacatc
    18121 gggccggaag tgccgaaaga agatctgatc tggcaagatc cgctgccgca gccgatctac
    18181 aacccgaccg agcaggacat tatcgatctg aaattcgcga ttgcggattc tggtctgtct
    18241 gttagtgagc tggtatcggt ggcctgggca tctgcttcta ccttccgtgg tggcgacaaa
    18301 cgcggtggtg tcaacggtgc gcgtctggca ttaatgccgc agcgcgactg ggatgtgaac
    18361 gccgcagccg ttcgtgctct gcttgttctg gagaaaatcc agaaagagtc tggtaaagcc
    18421 tcgctggcgg atatcatcgt gctggctggt gtggttggtg ttgagaaagc cgcaagcgcc
    18481 gcaggtttga gcattcatgt accgtttgcg ccgggtcgcg ttgatgcgcg tcaggatcag
    18541 actgacattg agatgtttga gctgctggag ccaattgctg acggtttccg taactatcgc
    18601 gctcgtctgg acgtttccac caccgagtca ctgctgatcg acaaagcaca gcaactgacg
    18661 ctgaccgcgc cggaaatgac tgcgctggtg ggcgggatgc gtgtactggg tgccaacttc
    18721 gatggcagca aaaacggcgt cttcactgac cgcgttggcg tattgagcaa tgacttcttc
    18781 gtgaacttgc tggatatgcg ttacgagtgg aaagcgactg acgaatcgaa agagctgttc
    18841 gaaggacgtg accgcgaaac cggcgaagtg aaatacacag ccagccgtgc ggatctggta
    18901 tttggttcta actctgtcct gcgtgcagta gcggaagttt acgccagcag cgatgcccac
    18961 gagaagtttg ttaaagactt cgtagcggca tgggtgaaag tgatgaacct cgaccgtttc
    19021 gacctgctgt aatctgacct tcttcagcga ctgtctttca ggcagtcgct gaagtttctt
    19081 taccggcgta tagtgtccac aggaaaacta cacactggat ctctcatgtc tgccgcagga
    19141 aagagcaacc cactggcaat cagtggcctg gttgtgctca cacttatctg gagttatagc
    19201 tggattttca tgaagcaagt caccagttac atcggtgcct tcgactttac cgccttacgc
    19261 tgcattttcg gcgctctcgt tttattcatc gtccttttat tacgtggtcg cggaatgcgc
    19321 ccgacgccgt ttaaatacac cttagccatt gccctgttac aaacctgcgg gatggttggt
    19381 ctggcgcagt gggcgttggt cagcggaggt gcggggaagg tggcgatcct gagctatacc
    19441 atgccgttct gggtggtgat tttcgccgcg ttgtttctcg gtgaacgcct gcgacgtggg
    19501 caatatttcg cgattctgat tgccgctttc ggcttatttt tggtgttgca gccgtggcaa
    19561 ctcgatttct cttcgatgaa aagtgccatg ctggcaatcc tctccggtgt tagttggggg
    19621 gcgagcgcga ttgttgctaa acgcctatac gcccgtcatc cgcgcgtgga tttattatcg
    19681 ttaacatcct ggcagatgct gtatgcggcg ctggtgatga gtgtggtcgc tttactggtg
    19741 ccgcaacgtg aaattgactg gcagcccacc gtgttctggg cgctggccta cagtgcgatt
    19801 ctggcgacgg cgctagcgtg gagcttatgg ctgtttgtgc tgaaaaactt gcctgccagt
    19861 attgccagct taagcacact ggccgttccc gtttgtggcg tactcttttc ctggtggctg
    19921 ctcggtgaga atccgggggc cgttgaaggt agcggtattg tgctgattgt gctggcactg
    19981 gcgctggtga gccgtaagaa aaaagaagct gtcagtgtaa aaagcatctg aattttttct
    20041 tcatgtgggg cgatctctta tttaacaaaa taacgataat gccccaccat ccgccagcta
    20101 aacagcacat cttcttcctg cgcgcctgcg ccaatgttat gtatcaccag cggcgtaccg
    20161 tcgcgggcga agccatctga cgccacccca atatgcgcca gcccgttatc taatcgccag
    20221 gagacaatat cgcccgcttg atagtcaccg gcatttttac tgatggggcg tgttttgttg
    20281 tggcggctaa accaggtttc cagattaggc acccgacggt gatcgatgtt gctgtccggg
    20341 cgctttaact tccatttttg cgggtactca gcaaaattct tcgccatatc ttcgtgaacc
    20401 agtttctgca aatcgacctt ctggctgcgc aatgcgcgga tcaccacatc ggaacataca
    20461 ccgcgttctt gcggaacatc accgccagga taggtaagct gcatatacgc cggatcgtaa
    20521 aatagcgtgc tgccaatttg ctgtctggca ccgtctgcga tggcaaggtt ggtattggcc
    20581 tggatttgta ccacggttgg tggaacggcg ggagatttta aggagtggct ggtaaatgcc
    20641 gttagcaggc tgagcagcgc cagtgaagct ttcatctggt tcgtccgtga aaataagagt
    20701 cacagattcg accttccggg ctaacgattg ttctgtggag aaaatgtcca gaagcggata
    20761 aaaaactcct gaacaacgtg ggagtttcag attactggca cagagtgccg gatgcggagt
    20821 gaacgcctta tccggcctac aaaagcacgc aaattcaata tattgcagag attgcgtagg
    20881 cctgataagc gtagcgcatc aggcaatttt gcgttcaaac tcccggacaa gccgggagtt
    20941 tggagtaggt tattcccact cttgcaggaa acgctgaccg tactggtcag ctaccagcag
    21001 tgcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcaccttc
    21061 tgcacaagat gcttttgcaa caatgcgcat tttcgccggg acatcttctt taatatccag
    21121 ttgagcgaga gttattggca aacctaccgc atggctaagt gcagctacgg tttcgatttc
    21181 ctccaccggc gcgttttcca gtaccagctg cgtcagcgta ccaaatgcca ctttttcacc
    21241 gtgataatag tgatgcgcgt ccgggatagc ggtcaggccg ttatgcaccg cgtgagccgc
    21301 cgctttcaaa accaacgccg ctcaaatagg tgttcgcttc aatcacgcgc tccagcgccg
    21361 gggttactac atgctgttcg gcggcaagca tcgctttttc gccttcttcc agcagggtgt
    21421 tgtagcacag ttcagccagt gccagcgccg cctgggtgca cttgccgccc gccatggtgg
    21481 tcgcgccgct acgagagcaa gcacgcgctt caaaccaggt tgccagcgca tcgccgatac
    21541 ccgctgctaa cagacgtgca ggtgcgcccg cgacgatttt ggtgtcgaca atgaccatat
    21601 tcgggttatt tggcaacagc agatagcggt caaactcacc ctcatcggtg tagataacag
    21661 acaatgcgct gcacggtgca tcggtagagg cgatagtcgg tgcgatcgct accggaacac
    21721 ccatgaaatg tgccagtgct ttggcagtat cgagggtttt tccgccaccg ataccgagaa
    21781 ttgcgccaca ctgcgcagtc tccgcgatgc cacgcagacg gtcgatctca ttttgcgaac
    21841 attcaccgcc aaacggcgta atttctacta ccagtccagc atctttaaag cttttctcga
    21901 cagtggattg agcaaaacct aaaacaaatt tgtcacccac cactaaccag cgttctgcca
    21961 gcggcttcag gtattcgccc agacgattaa tcacatcagc gccctggatg tatttacccg
    22021 gtgattgaat aatgcggtcc ataattgctc ctttagagat gagtagtgcc aaatgcggca
    22081 ttccagtcgt gttcgaactt ctctatagct gactctactg caggggtgtt gagcatttgt
    22141 tgcgctacat ctaagggcag ggtgatggat tcacatcctg ccagtaaaca gtccagcgcc
    22201 tgacacggcg ttttaaagct ggctgccagc accatgcttt ctggcgcgtg catttctaac
    22261 agcgtttgca gctcctgaac cgtacgaatg ccgtccccgc cctgggcatc tacgcggtta
    22321 acatacggag caacgtattt tgcccctgcc agtgcggcta ataacccttc tgcggcgcta
    22381 tatacagcgg tgccaagtgt agtaataccc tcttttttca gtattttaat tgctgccaga
    22441 ccttcggaag tcaccgggat tttcaccaca atacccggaa tagcgtcgcg caggcgcttc
    22501 gcttcttcca ccatcccctg cgcgtcgcgg ctcatggtct gagcaaacag aatgccctca
    22561 tcaccaatcg ctttttgcag acgcggcagc acttcccaga tggactcctt gctggcagcg
    22621 ataatgctcg ggttagttgt caccccggca atcgggaata tgcgtgccag acgttcgact
    22681 tctgcgacgt tagcggtgtc cagatacagt tccatgatgt ttccttttac agttccagtt
    22741 catgttgcag caggctggcg atagcgtcgg cagaggcggc gttaaccagc gcgttacgga
    22801 attcttcgtg catgatgcgg cgagcgaggc gcgagaaaat gcgcatgtgc tgatcgcccg
    22861 cagcgtgttt gttcagggtt aacataatga tgaattgcgc ttcatcatcg ccccagcgca
    22921 ccggcgcttg cagacgcgcc acgctgatgg tggattgctc aatgtgttct gatttgctgt
    22981 gtggaatggc aaaactaaag cccagaccgg tagagaaaac ggcctcgcgc gcccataagt
    23041 cggcttccag tttacgcgga tagcggcagc ggcccgccag cagcaggtta tcggtcatgc
    23101 ctttgagcac ttcttctttg ctgcgccagt cactttccag cgtgatgcac tcggcggtga
    23161 ccagcggtgc gtcctgttgg gtcatgcgga attgcgccag caggtgttcc acttccagcg
    23221 aagtacggca ggccattgcc tggttgagca acttgcggca ctcacggcta tcaagttgcg
    23281 ccatccgcgc tttcgccgcc ggaattgatg gtgcgctcat gctgagttca tccagcccta
    23341 agccgaccag caacggcagc acggaacctt tcgctcccag ctcaccgcac agaccaatcc
    23401 atttaccctg gcgatgcacc gcctgcacgg cgtaatcgag cgcccgcaag aatgccggat
    23461 tcaggctgtt gtagtgacga gtaaccttag cgttatcgcg atccactgcc agcagatatt
    23521 gcgtcaggtc attactacca atactaaaga aatcaatctc ttcgcagcat tgatcgatga
    23581 tgaacatcac cgacggcact tccagcataa tgccgagctg gatcttctca tcaaacggaa
    23641 tgtgttcgtt acgtagttgc tgtttggctt ctgccagttt ttctttcacc cataagatct
    23701 cttccattga ggagatcatc gggatcatga ttttcaggct gccgtgagcg gaggcgcgca
    23761 ggatcgagcg tagttgtgtg gtaaataacg acgcgtattc ttcataaata cgcacggcgc
    23821 gataaccgag gaacgggttt gcctctgcgg gaatgtttag ataatcaacg ggtttgtcgc
    23881 cgccaatgtc catagtgcgc acaataatgc tgcgtccgtt ggcagactcc agcgcctgac
    23941 aaaaaatgtt gtacagctcg ctttcgcccg gtgcactggt gcgatccata tagagcattt
    24001 cagtgcggaa caaaccaacg ccttccgcac cattgccgaa tgcggcctgc gcttccacgg
    24061 agtgagcgat gttagcggca atttcaatgc ggataccgtc agcggtacgg gcttgttggg
    24121 tcagccagac acgctgttgc tcacgcaggg cgtcctgtac gcgggcttct tgctgataat
    24181 aacgagctac ggcttctcct ggctcaacca caatcgcccc ggcgttgccg tcgatataaa
    24241 tcgtttgatg ctgccacgga gtgagggcat caatatccac accaaccagc gtcggaatgt
    24301 tgaacgaacg ggcaaggatc accgtatgtg aggtggtgcc gccgcttttc agtaacaatc
    24361 ctttgaggtg atttttatcc agttcgagga actggctggg ggtcagttca tcagccatac
    24421 aaatggcgga ctgcgtcagt ttgcccggtg ccgggaagcg ttgctcaccg tagatttgct
    24481 ggagtaactg gaagcagacg tcgcgtacgt ccagggcacg ttcttgcagg tatctgctgc
    24541 tggaacgggc gaactcttcg caaaagtgat tcgcactggc gacaatcgct tcggcgcagc
    24601 ttaatccggc gctgactcct gccagtaaat gttcgtgcag ggaagtatcg ccagccaggg
    24661 atcggtgcgc ttccagaatc gcgctggtag caccgtcgct atccagcaga cgaaactcaa
    24721 tgtttttcag taccagcgtc aggccgtttt ccagtgcgga ttgctcggcg tcaacgtctt
    24781 tggctgcggg aagattactc agcgcattga gatctaaaga agagatcggc gtcagaatgc
    24841 cgcctgcact accgctgcac acggtgcgga cgcggataat ttgcggattc agattggtca
    24901 gtgaaaccgg cagtggttcc agttcgtcag atttaacttc cgccagcggc gcatcgcagt
    24961 gggggaattc atcgcgcagc cattggctta aacgctggtg cccttcctgt tcgtcggccc
    25021 cggaaatcag tagctggcag ttatcaccca ccagcgtatc ggtgccaatc agcgccagag
    25081 cgcttttggc gttgccttta cggtcagtac gcaggttatg ccactcaatt tgagatgaaa
    25141 aagtattaca cagcgtttca acgtggcttg ccggacgcgc atgtacgccg ttaggtagct
    25201 cacaaataaa ttccacaatc agggccattg cctttctccc ggtacggttt tctgtttaca
    25261 ggataagagc gcacggtaac ggcctgccat gtgacaaatc tgccaaaagc tggacaaatg
    25321 taatgtaacc gtcaatttgc gacgcgtctc acaagacgcc gttttgcggc atgcttccgg
    25381 attatcgcaa gttatgaggc ggatcgcatt tttgtactga tattacaaaa gtccagtaaa
    25441 tggccttttt atccactgtt tgctccgcct gcgattgcct attgtttcct ccatcaaaaa
    25501 tatggacatg ggcttttgca agcccgggag cagcttatga atgagttggt gcagatcctg
    25561 aaaaataccc gtcagcattt aatgacgggc gtttcacaca tgattccctt cgtggtatcg
    25621 ggcggtattt tgctggcggt ttccgtcatg ttgtatggca aaggcgcagt gccggatgcc
    25681 gtagccgatc cgaatttgaa aaaactgttt gatattggcg ttgcaggttt gacgctgatg
    25741 gtgcctttcc tcgccgctta cattggttac tccattgcag agcgttctgc gctggctccg
    25801 tgcgctatcg gtgcctgggt tggtaacagc tttggtgcgg gcttctttgg tgcgctgatc
    25861 gccgggatta tcggcggcat cgtggtgcat tacctgaaga aaattccggt gcataaagtt
    25921 ctgcgctcgg tgatgcccat cttcattatt cctatcatcg gcacactgat taccgcaggc
    25981 atcatgatgt ggggcttggg cgagcctgta ggggcgttga ccaacagcct gactcagtgg
    26041 cttcagggga tgcagcaggg cagcattgtt atgctggcgg tgatcatggg gctgatgctg
    26101 gcgttcgata tgggcggtcc ggttaacaaa gtggcctatg ccttcatgct gatttgcgtt
    26161 gctcagggtg tttataccgt ggtggccatt gctgccgtgg gtatttgtat cccgccgctg
    26221 gggatggggc tggcgacgct gattggtcgt aaaaatttct ccgcagaaga gcgcgaaacc
    26281 ggcaaagcgg cactggtgat gggctgtgtt ggcgttactg aaggggcgat tcctttcgcc
    26341 gctgccgatc cgctgcgtgt cattccttcc attatggtcg gttcagtttg tggtgcagta
    26401 actgcggcgc tggtcggtgc gcagtgctat gcaggctggg gtggtctgat tgtgctgccg
    26461 gtggttgaag gtaagttggg ttatatcgca gcagtggctg tcggagcagt ggtgacggct
    26521 gtttgtgtga acgtgctgaa aagtctggcg cgtaaaaatg ggtcttcgac tgatgaaaaa
    26581 gaagacgacc tggatttgga ttttgaaatt aattaattga ggaacgagcc atgacgaaaa
    26641 ttattgcagt aaccgcatgt ccttcaggtg ttgcccatac ctatatggta gcagaggcgc
    26701 tggaaagtgc cgcgaaagcg aaaggctggg aagtgaaagt agaaacccag gggtcaattg
    26761 gtctggaaaa tgaactgact gcggaagacg tggcgagcgc tgatatggtg attctgacca
    26821 aagatatcgg catcaagttt gaagagcgtt ttgctggcaa gaccatcgtg cgcgtcaaca
    26881 tcagcgatgc agtaaaacgt gctgacgcca ttatgagcaa aattgaagct cacctggcgc
    26941 aaaccgctta agttcttctc ccgctcgcaa gggcgggttc gctttctcac aggagttcct
    27001 catgacgaat cgtatctctc gcctcaaaac tgcactgttt gccaataccc gtgaaatctc
    27061 gctggagcgg gcgctgcttt ataccgccag ccatcggcaa accgaaggcg aaccggtgat
    27121 attgcgccgg gcgaaagcaa cagcgtatat ccttgaacat gttgaaattt cgattcgtga
    27181 tgaagaactg attgccggta accgcaccgt aaaaccgcgc gccgggatta tgtcgccgga
    27241 aatggaccct tactggctgc tgaaagagct ggatcaattc ccgacgcgtc cgcaggaccg
    27301 ctttgctatc agcgaagaag ataaacgtat ctaccgtgaa gagttgttcc cgtactggga
    27361 aaaacgttcg atgaaagatt tcatcaacgg gcagatgaca gatgaagtaa aagccgcgac
    27421 caacacgcag attttcagca tcaaccagac ggataaaggc caggggcaca ttattattga
    27481 ttacccacgc ctgttgaatc acggtctggg tgagctggtg gcacagatgc agcaacattg
    27541 tcagcaacag ccggagaatc acttttatca ggccgcgttg ttactgctgg aagcctcgca
    27601 gaaacacatt ttgcgttacg ccgaactggc ggaaacgatg gcggcaaact gcacagatgc
    27661 ccagcgtcgc gaagagctgc tgactattgc ggagatctcc cgccataacg cgcaacataa
    27721 gccgcagacg ttctggcagg cgtgccagtt attctggtac atgaacatca ttctgcaata
    27781 cgaatccaac gccagttcgc tgtctttagg gcgcttcgac cagtatatgt tgccgttcta
    27841 tcaggcatct ttaacccagg gcgaagatcc ggcgttcctg aaagaactgc tcgaatcttt
    27901 atgggtgaaa tgcaacgaca tcgtgctgtt gcgctccacc agtagcgcgc gttatttcgc
    27961 cggtttcccg accggctata ccgcactgct cggcgggtta accgagaacg gacgtagcgc
    28021 ggtgaacgtg ctttcgttcc tttgccttga cgcctatcaa agcgtgcaat taccgcaacc
    28081 gaacctcggc gtgcgcacta acgccttgat cgacacgccg ttcctgatga aaaccgccga
    28141 aaccattcgc ctcggcaccg gtattccgca aatctttaac gatgaagtgg tggtgccagc
    28201 gttcctcaac cgtggcgttt cgctggaaga tgcgcgcgac tattccgtag tgggctgtgt
    28261 ggaattatct attcccggca gaacctacgg cttgcatgac atcgcgatgt ttaatctgct
    28321 gaaagtgatg gaaatctgcc tgcatgaaaa tgaaggcaat gctgcgctga cttatgaagg
    28381 tttactggag cagatccgcg ccaagatcag ccactacatc accctgatgg ttgagggcag
    28441 caatatttgt gatatcggcc atcgcgactg ggcacctgta ccgctgctct cgtcttttat
    28501 cagcgattgt ctggaaaaag gtcgcgatat taccgatggc ggcgcgcgtt ataacttctc
    28561 cggcgtacag ggaattggta tcgccaacct gagcgattct ctccatgcgt tgaaagggat
    28621 ggtttttgag caacagcgtt taagttttga cgaattgctg tcggtattaa aagccaactt
    28681 tgcaacgcca gaaggcgaaa aagtccgcgc tcgcttaatt aaccgctttg agaaatacgg
    28741 taacgatatc gacgaggtgg ataacattag cgccgaactg ttgcgccact actgcaaaga
    28801 agtggaaaaa taccagaacc cgcgcggcgg ttacttcacg ccgggatcgt ataccgtttc
    28861 tgctcacgtt ccgttgggat cggtggttgg cgcgacgcca gacggtcgtt ttgccgggga
    28921 acaactggca gacggcggct tgtcacctat gctgggtcag gacgcacaag ggccaacggc
    28981 ggtactgaag tcagtcagta agctcgataa tacgctgctg tctaacggta cgttgctgaa
    29041 cgtgaaattc actccggcga ccctggaagg tgaagcggga ttacgcaaac tggccgactt
    29101 cttacgggtg tttacccagc ttaagttgca gcatattcag tttaacgtgg tgaacgccga
    29161 cacgttgcgg gaagcgcaac agcgcccaca agattatgcc gggctggtgg tgcgcgttgc
    29221 tggatacagc gccttctttg tcgaactgtc gaaggagatc caggatgaca tcatccgccg
    29281 gacagcgcat cagctgtaac gttgtggaaa cgcgccgcga tgatgtggcg cgcattttca
    29341 acattcagcg ttattcactg aatgacggcg agggcattcg tacggtggtc ttttttaaag
    29401 gctgtccgca tctttgcccg tggtgtgcta atccggagtc gatctccggc aaaatccaga
    29461 cggtacgcag agaggcgaaa tgtctgcact gtgcgaaatg tttgcgtgat gcggatgaat
    29521 gcccctccgg ggcgtttgaa cggattggtc gcgatatcag ccttgacgct ctggaacggg
    29581 aagtgatgaa agatgacatt ttctttcgca cgtccggcgg cggcgtcacg ctttctggcg
    29641 gcgaagtgtt aatgcaggcg gagtttgcta cccgtttttt acagcgactg cggctgtggg
    29701 gtgtctcatg cgccattgaa actgccggag acgcgccagc aagcaagctg ttaccgctgg
    29761 cgaaattgtg cgatgaagtg ctgttcgatt taaaaattat ggacgcgact caggcgcggg
    29821 atgtggtgaa gatgaacctg ccacgcgtgc tggagaatct gcgtttgctg gtgagtgagg
    29881 gtgtcaacgt gatcccgcgt ttaccgttga tccctggttt cacgctcagc cgggagaata
    29941 tgcagcaggc gctggatgta ctgatcccgc tgaaaatcag gcagatccat ctgttaccgt
    30001 ttcatcagta cggcgaaccg aaataccgcc tgctggggaa aacatggtcg atgaaagagg
    30061 tgcctgcgcc gtcgtcagcc gatgtgacaa cgatgcgcga aatggcagaa cgggccggat
    30121 ttcaggttac cgtgggaggt taaaatggca tacctggtgg cagtaaccgc ctgcgtaagc
    30181 ggcgtggcgc atacttatat ggcggcggaa cggctggaaa agttgtgcca gttagagaag
    30241 tggggcgtca gcattgaaac tcagggcgcg ctgggaacgg agaatcgttt agcggacgag
    30301 gatatccgtc gggcggatgt tgctctgttg attacggata tcgagcttgc cggtgccgag
    30361 cgatttgaac attgccgcta tgtgcaatgc agcatctacg cattcctgcg tgagccgcag
    30421 cgggtaatga gcgcggtgcg caaagtgctt tctgcgccgc agcaaaccca tcttattctg
    30481 gagtagtcgg tttttctgtc agttggctgt ggtactgccg gcgatattcc gacggcgagt
    30541 gttcggtgtt tttacgaaac agacggcaga agtagttgct gtcgacaaag ccgcaggcgt
    30601 gcgccacttc ttttaccttc aggtcatagc ctttcaataa cgtcttagcg tgctccagtc
    30661 gcgtgtgatt caggtattcg ttaaaaccaa tggcccccgt tttttgaaac aggtgcgaga
    30721 gatagtttgg tgaaatatag aatgcctgtg cgacagattc gcgggtaagc gcggaggcat
    30781 agcgttcgtc gatataatcg cgaatagctt caaatagtgc ctggctgcgt gaggcggtct
    30841 ggatttggct gccgcgcaaa tcgcggcagt ggctaagcag gctggctacg ataagccttg
    30901 ccgtttgctg ctcctgcggc tgcatttgca tttcattgag cgtttgtagc agaaaagaac
    30961 ctatgcgtgg gccgcgtcgg gcgacgtgtt gcttcgccag attttgatat tgtttgccat
    31021 cccattgcac gacgctgaag ccgagttgct gtttgccaaa cagcacgcta aaggtggtag
    31081 cgggtgtttt ccattgtggg aaattccagc caccagcggc aacgtacagc acatcgccgg
    31141 gaactaacgt tgctccagcg ccggtatcaa caaactcacc ttccagcaca atttccagcc
    31201 gtggaaaatc gacctgatac gcaagatcgg gcaccgggcc gttactgctg gcaaagtaaa
    31261 tctggcgcag ggacagcggg ccgttgatca ggcgggagag caaatagctg acgtcgtgat
    31321 acatgttatt cccttaggta tgtagacggg aaaacagtaa ctggcattat taaaaaaggc
    31381 cacctgaaaa gtgtggcctg aggggagttc gatgtaaaca ttgcggcagt atcgcatccg
    31441 gcaaatagcg ccagacgcta tacaaaggtc atctactgat tacccacctg atcgccatac
    31501 ggcagagtgt cgtaatcgat cagcgcgttt ttcttgtacg ggttaccaat ccagcgggta
    31561 gtttctttga actgcggatt caccactgaa cgggttggat cgtaaccgtc gtaagataag
    31621 cccgccaaat ctgaccaggt gtggatcagt tccgccaggc tgtatttacg atcgacatcc
    31681 tgtgagaaat cacggggatg agtcgcttgc catttttccg acgtccacag caggaacgga
    31741 atggtgtaca tgtggcgcgt cgggttgtct tcgttacgcc cctgagtttt gtgcggcggc
    31801 gtgtcgtaaa cctcttcacc gtggtcagag aagtaaacca ggaatccgtt cgggtctgcc
    31861 gctttaaagt ctttaatcag gctggcaacc acatgatcgt tatacaggtt agcgttgtca
    31921 taatcgttat atgattccag ctcttctgcg cttaatcctg gaggaacatg atcggtattg
    31981 ccatcaaact tgccctgatt ttccgggtag cggtatttgt atttgatatg cgtacccagc
    32041 agatgaacga tgatcagttt cttcggcgca gggtcattca gcacatcctg gaacggcttc
    32101 agcacgttgg tgtcgtattc acgcgcactc tgcgtacgtt gctggttcat gtagtactgc
    32161 ttgtcggtct ggcgcgaaaa taccgtcagc atggtattgc gggcggtcat cgtctgctgg
    32221 ttggtgatcc agaaggtttt ataacccgcc tgtttcatca tgttcatcag cgacggctgc
    32281 gtcagataca gatccgggtt cttttcattg gcaaaggtca gcgcctgttg caggatttca
    32341 atggtgtacg gacgagacgt aactacgtta ttaaacacgg tcagattcgg atcggtttta
    32401 tgcagcgcat ccagctccgg cgtggtttca cgcggataac cgtacagact catgcgtccg
    32461 cgctgggtcg actcgccaat caccagcact aaagtgcgcg gttcgttacc cgattcatct
    32521 ttgaaattag ccagtggcgg caaggcatta ttttcattca gtaactttgt tagcgagttt
    32581 agttgctgac gatactgata atagccggtc aggaattgcc acggtgcggc aggctccata
    32641 cgcgaggcta ggttatccaa cgttttctca aacggcttgt ttttgataaa cgtattcatg
    32701 gcgatcggat gcagaatcaa gccataaagc agggcaaaag agacaacata acgccacggc
    32761 tttggaatat agaccgggcg caggcgtgtc cacagcagaa ctgccaccgc cgtataggcc
    32821 agcgcgataa gcacaatttt caggctgaaa tactggctta aatactcgct ggcttcgttg
    32881 gtgttggttt cgaacatcac aaacagaacg ctctgcgaga actcctgccc gtagatgacg
    32941 tagtagcaca gcgccgccag agaggccgtc catagcacca cgccgatcac tgcggcaata
    33001 attttaatcc gcttcggaaa gaggaatacc gggatcaacc acagcgagct gaataacagc
    33061 gagtcgcgaa tgccgttggt gccactataa ccactgatgt aaataatggc ctgtagcaga
    33121 gtagagaaaa accaaaagta gagcagtgcc caacccaggg ctttccagct agaaaggggt
    33181 ttagcctgga cttctgtgga atgcatagta agaacctgtc ttgaaaaaaa tatcgccgaa
    33241 tgtaacgaca attccttaag gatatctgaa ggtatattca gaatttgaat aaaatgcaga
    33301 cagaaatata ttgaaaacga gggtgttaga acagaagtat ttcagaaaac cctcgcgcaa
    33361 aagcacgagg gtttgcagaa gaggaagatt agccggtatt acgcatacct gccgcaatcc
    33421 cggcaatagt gaccattaac gcttgttcga cgcgaggatc cggttcctgg ccttcttttt
    33481 ctgcctggcg ggagcggtgc agcaactcgg cctgcaatac gttcagcggg tcggtgtaaa
    33541 tattccgtag ctgaatagac tctgcaatcc acggcagatc ggccatcaga tgggaatcgt
    33601 tggcaatcgc cagcaccact ttgatgtctt cttcttgcag gttgcgtaac tctttaccta
    33661 acggccacag tgctttgtct accagacgtt gatcatagta ttccgccaac cacaggtctg
    33721 ctttggcgaa gaccatctcc agcatgccga gacgcgtcga gaagaatggc caatcgcggc
    33781 acatggcttc cagctcgctc tgtttgccat cttcgaccac tttttgcagc gccgtacctg
    33841 cacccagcca ggcggggagc atcaggcggt tttgcgtcca ggcaaaaatc cacggtatgg
    33901 cgcgcagcga ctcgacgccg ccggttgggc gacgtttcgc cggacgtgaa cccaacggca
    33961 gtttgcccaa ttcttgttcc ggcgtagcgg agcggaagta aggcacaaaa tctttgtttt
    34021 cacgtacgta gccacggtag agatcgcagg agatgactga cagttcatcc ataatgcgac
    34081 gccagctctc tttcggctca ggtggtggca gcaggttggc ttccagaatc gccccggtgt
    34141 aaagcgacag gctgctgacg gtgatttctg gcagaccata tttaaagcgg atcatctcgc
    34201 cctgttcagt cacgcgcagg ccacctttca ggcttcctgg cggttgtgac agcagcgccg
    34261 catgggcagg tgcgccgccg cgaccaatgg aaccgccgcg accgtggaac aacgtcagct
    34321 caatacccgc tttttcgcag gttttgatta atgcatcctg tgcctgatat tgcgcccagg
    34381 aagctgccat cacgcccgca tcttttgctg agtcggaata gccaatcatc accatctgtt
    34441 tgccctgaat caggccgcga taccagtcga tattgagcag ctgggtcatg acatcgttgg
    34501 cgttgttcag gtcatcgagg gtttcaaaca gcggagcaac cggcatcgca aacccgatac
    34561 ccgcttcttt cagcagcagg tggacagcca gtacgtcgga cggcgttttc gccatcgaga
    34621 tcacgtaggc ggcaatggag ccttgcggcg cttcggcaat cacctggcag gtatcgagca
    34681 cttcgcgcgt ttcggcgctt ggttgccagt tgcgcggcag aagcggacgt ttggagttca
    34741 gttcgcggat caggaacgcc tgtttgtcgg cctctgacca gctttcgtag tcgccgatac
    34801 cgaggtagcg ggtcagctcg cccagcgctt cggtatggcg ggtgctctcc tgacggatat
    34861 caatacggac cagcggtacg ccgaaacatt tcacgcggcg cagggtgtcg agcagatcgc
    34921 cgttggcgat aatacccatg ccacacgcct gaagtgactg gtagcaagcg tagagcggtt
    34981 cccacagttc ttcgttttgt gtcagcagcc cttctggttt tggcagttct tcgcctttca
    35041 ggcgcgcttc cagccatgcc tgtgtcgcca tcaggcgaga acgcaggttt ttcatcagat
    35101 agcgatacgg ttctgcggca ccttcttcgc caaccagcgc cagcagttca ggggtcgctt
    35161 caaccatcga cagttcagaa accagaacct gaatatcttt caggaacaga tcggtggctt
    35221 tccagcggct gagcagcagg acgtggcggg tgatatcggc agtgacgttc gggttgccgt
    35281 cgcggtcacc gcccatccac gaggtaaaac gtaccggaac aaattcgacg ggcagtttgt
    35341 agtcgagatt ctcttccagt tgttcgttca gttcgcgcag gtaattcggt acgccttgcc
    35401 acaggctgtt ttctactacg gcaaagcccc atttggcttc atctaccggg cttggacgca
    35461 gcttacggat ttcatcggta tgccatgact gggcgatcaa ctggtgcagg cgacgcatca
    35521 gctggttgtg ttcgtagtca gcgatatctt tgttatcgag ctgttttaaa caggcgttca
    35581 cttccaccat tttgtggatc agtgtacgac gggtaatttc ggttgggtga gccgtgagga
    35641 ccagttccag cgacagcgat tccactgctt ttttgatggt gtcttcgctc agttccggct
    35701 ggtttttcag tttacgcagg gtgcgggcga tcacttccgg gttgctggca gcttcgcctt
    35761 tcggcgaaat gctgtggtat tgctcggcgg tgttggccag gttcaggaac tgactaaacg
    35821 cacgcgcaac gggcagcagc tcgtcgttcg acaaattttg taaggtggtg agcaactcct
    35881 ggcggttagc atcattgcca gcgcgtgaag atttcgacaa cttacggata gtttctacgc
    35941 gttcaagaat gtgttctccc aacgcatcct tgatggtttc tcccagcact ttgccgagca
    36001 tactgacatt actacgcaat gcggaatatt gttcgttcat attaccccag acaccccatc
    36061 ttatcgtttg atagccctgt atccttcacg tcgcattggc gcgaatatgc tcgggctttg
    36121 cttttcgtcg tcttttataa agccacgtaa aagcggtgac gtcaaatgct gcgaaatcgc
    36181 ttcagcaaac gaataaatag caggaattta cgccattaaa ttcacgacgc tttaaataag
    36241 cgtaacttat ggaaatgtta aaaaatcgcc atacataaca ccaaaggtgt aggtcggata
    36301 agatgcgcaa gcatcgcatc cgacattatt gcggcaccgg agtttggcaa cggtgccgga
    36361 tgcggcgcga gcgccttatc cggcctacgt tttaatgcca gcaaaaatgg tgaattacct
    36421 ggataatcaa ctcacgggtg ggtttaataa accgcgtctc cagatattca tcaggctgat
    36481 gagcttgatt gatggaaccc ggaccgagca ccagcgtcgg gcataacgtt tgaataaacg
    36541 gcgcttcggt acagtaattc accacttcgg tttttgctcc gagcaatttc tcaaccactt
    36601 caaccagttg atgattcggt gggcattcat agccagggat cggcggatgc agctcgtcga
    36661 ccgtcagacg acccggccag cgttcgctca ccggagccaa tgcatcgttg agcaaaccat
    36721 taagttcatt gagtgtcatg ccaggctgcg gacgaatatc catatgcaac tcacagcaag
    36781 cgcaaatacg gttagaagcg tcgccaccgt gaatatgccc gaggttgagc gtagggtatg
    36841 gcacggtaaa tgcttcgtag tgataacgtt ctttcaggtt atcgcgcaat tgcaaaatat
    36901 gcccgatggc gtcgtgcatt agttcgatag cgttaactcc gcgtgctgga tcgctggagt
    36961 gccccgactg gccctgaata cggatggcgt tagagatatg acctttatgt gcgcgtaccg
    37021 gttgtagtga cgtcggttcg ccaatgatgg cgcaatccgg gcgcagggcg gtagtttcgg
    37081 caaaataacg cgctccggcc atactggttt cttcatcagc agtcgccaga atgtagagcg
    37141 gttttttcag tttcgtgacg tcgacatcgc gtagcgcatc aaggataaac gcaaaaaagc
    37201 ctttcatgtc ggcggtgcct aagccgtaaa gcttgccgtc atgctccgtc agtgtaaacg
    37261 gatcgcgcgt ccagcgaccg tcatcaaatg gcaccgtatc ggtatgcccc gccagcaaca
    37321 agccgccagc cccctgtccg taactggcca gcatattaaa tttgttgcga gttcctggaa
    37381 caggctgcac ttccacattg aagcccaaat ctttaaacca gtccgccagc agagtgatta
    37441 aatctgcatt gctttgatcg agtgcctctt ccgtagcgct tattgaaggt gtggcaatca
    37501 gagcgcggta aatctcgata aatggcggta atttgttttt cattgttgac acacctctgg
    37561 tcatgatagt atcaatattc atgcagtatt tatgaataaa aatacactaa cgttgagcgt
    37621 aataaaaccc accagccgta aggtgaatgt tttacgttta acctggcaac cagacataag
    37681 aaggtgaata gccccgatgt tgaatacgct gattgtgggt gccagcggct acgctggcgc
    37741 agagctagtg acctatgtaa atcgccatcc gcatatgaac ataaccgctt tgactgtttc
    37801 agcgcaaagc aatgatgcgg gaaagttaat ctccgatttg catccgcagc taaaaggcat
    37861 cgttgagctg ccgttgcagc cgatgtcgga tatcagcgag tttagcccag gggtggacgt
    37921 agtgtttctc gccaccgccc atgaagttag ccacgattta gcgccgcagt ttcttgaagc
    37981 gggctgcgtg gtgttcgacc tttccggcgc gtttcgtgtt aacgacgcca ccttctatga
    38041 aaaatattac ggctttaccc atcaataccc ggaactgctg gaacaggcag cctacggtct
    38101 ggcggagtgg tgcggtaata aattaaaaga agcgaatttg attgcggtgc caggctgtta
    38161 tccgacggcg gcacagctgg cgctgaaacc gttgattgat gccgatcttc ttgacctcaa
    38221 tcagtggccg gtgatcaacg ccaccagcgg cgtgagcggt gcaggacgta aggcagcgat
    38281 ttcaaatagt ttttgtgaag ttagcctgca accgtatggc gtctttactc atcgccatca
    38341 accagagatc gccacacacc tcggtgctga cgttatcttc accccacatc tgggcaattt
    38401 cccgcgcggc attctcgaaa ccattacctg ccgcctgaaa tcgggtgtga cccaggcgca
    38461 agtcgcgcaa gcgttacagc aggcgtatgc ccataaaccg ctggtgcggc tgtatgacaa
    38521 aggcgttccg gcgctgaaaa atgtcgttgg gctgccattt tgcgatatcg ggtttgccgt
    38581 tcagggcgag catctgatta ttgtggcgac cgaagacaac ttactgaaag gcgcggcggc
    38641 acaagcggta cagtgcgcca atattcgttt cggctatgcg gaaacgcagt ctcttattta
    38701 agggtgcaat gatgaatcca ttaattatca aactgggcgg cgtactgctg gatagtgaag
    38761 aggcgctgga acgtctgttt agcgcattgg tgaattatcg tgagtcacat cagcgtccgc
    38821 tggtgattgt gcacggcggc ggttgcgtgg tggatgagct gatgaaaggg ctgaatctgc
    38881 cggtgaaaaa gaaaaacggc ctgcgggtga cgcctgctga tcagatagac attatcaccg
    38941 gagcactggc gggaacggca aataaaaccc tgttggcatg ggcgaagaaa catcagattg
    39001 cggccgtagg tttgtttctc ggtgacggcg acagcgtcaa agtgacccag cttgatgaag
    39061 agttaggtca tgttggactg gcgcagccag gttcgcctaa gcttatcaac tccttgctgg
    39121 agaacggtta tctgccggtg gtcagctcca ttggcgtaac agacgaaggg caactgatga
    39181 acgtcaatgc cgaccaggcg gcaacggcgc tggcggcaac gctgggcgcg gatctgattt
    39241 tgctctccga cgtcagcggc attctcgacg gcaaagggca acgcattgcc gaaatgaccg
    39301 ccgcgaaagc agaacaactg attgagcagg gcattattac tgacggcatg atagtgaaag
    39361 tgaacgcggc gctggatgcg gcccgcacgc tgggccgtcc ggtagatatc gcttcttggc
    39421 gttatgcgga gcagcttccg gcactgttta acggtatgcc gatgggtacg cggattttag
    39481 cttaagtttt gttagccgga ggagcagctt tccggcattg aatttcaaaa taaggaaaca
    39541 gagttatggc actttggggc gggcgtttta cccaggcagc agatcaacgg ttcaaacaat
    39601 tcaacgactc actgcgcttt gattaccgtc tggcggagca ggatattgtt ggctctgtgg
    39661 cctggtccaa agccttggta acggtcggcg tgttaaccgc agaagagcag gcgcaactgg
    39721 aagaggcgct gaacgtgttg ctggaagatg ttcgcgccag gccacaacaa atccttgaaa
    39781 gcgacgccga agatatccat agctgggtgg aaggcaaact gatcgacaaa gtgggccagt
    39841 taggcaaaaa gctgcatacc gggcgtagcc gtaatgatca ggtagcgact gacctgaaac
    39901 tgtggtgcaa agataccgtt agcgagttac tgacggctaa ccggcagctg caatcggcgc
    39961 tggtggaaac cgcacaaaac aatcaggacg cggtaatgcc aggttacact cacctgcaac
    40021 gcgcccagcc ggtgacgttc gcgcactggt gcctggccta tgttgagatg ctggcgcgtg
    40081 atgaaagccg tttgcaggat gcgcttaagc gtctggatgt cagcccatta ggctgtggcg
    40141 cgctggcggg aacggcctat gaaatcgacc gtgaacagtt agcaggctgg ctgggctttg
    40201 cttcggcgac ccgtaacagt ctggacagtg tttctgaccg tgaccatgtg ttggaactgc
    40261 tttctgctgc cgctatcggc atggtgcatc tgtcgcgttt tgctgaagat ctgattttct
    40321 ttaacaccgg cgaagcgggg tttgtggagc tttcggaccg cgtgacttcc ggttcatcat
    40381 taatgccgca gaagaaaaac ccggatgcgc tggagctgat tcgcggtaaa tgcggccggg
    40441 tgcagggtgc gttaaccggc atgatgatga cgctgaaagg tttgccgctg gcttacaaca
    40501 aagatatgca ggaagacaaa gaaggtctgt tcgacgcgct cgatacctgg ctggactgcc
    40561 tgcatatggc ggcgctggta ctggacggca ttcaggtgaa acgtccgcgt tgtcaggaag
    40621 cggcgcagca ggggtatgcg aacgccactg aactggcgga ttacctggtg gcgaaaggcg
    40681 tacctttccg cgaggcgcac catattgtgg gtgaagcggt ggtggaagcc attcgtcagg
    40741 gcaaaccgct ggaagatctg ccactcagtg agttgcagaa attcagtcag gtgattggcg
    40801 aagatgtcta tccgattctg tcgctacaat cgtgcctcga aaagcgtgcg gcaaaaggcg
    40861 gcgtctcacc gcagcaggtg gcgcaggcga ttgcttttgc gcaggctcgg ttagagtaat
    40921 ttgtttatat tgctcccctg aaaatgggga gtttaccacg cctacaaagt gcgacacttc
    40981 gtaggcggat aaggcgttta cgccgcatca ggcagaaatt tgtcagcatt c
//
LOCUS       DAJTNM010000019        38433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTNM010000019 DAJTNM010000000
VERSION     DAJTNM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.019"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            343..393
                     /gene="hisL"
                     /locus_tag="OO172_001162"
     CDS             343..393
                     /gene="hisL"
                     /locus_tag="OO172_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5511419.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    369..492
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            539..1438
                     /gene="hisG"
                     /locus_tag="OO172_001163"
     CDS             539..1438
                     /gene="hisG"
                     /locus_tag="OO172_001163"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5511420.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1444..2748
                     /gene="hisD"
                     /locus_tag="OO172_001164"
     CDS             1444..2748
                     /gene="hisD"
                     /locus_tag="OO172_001164"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5511421.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2745..3815
                     /gene="hisC"
                     /locus_tag="OO172_001165"
     CDS             2745..3815
                     /gene="hisC"
                     /locus_tag="OO172_001165"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5511422.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3815..4882
                     /gene="hisB"
                     /locus_tag="OO172_001166"
     CDS             3815..4882
                     /gene="hisB"
                     /locus_tag="OO172_001166"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5511423.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4882..5472
                     /gene="hisH"
                     /locus_tag="OO172_001167"
     CDS             4882..5472
                     /gene="hisH"
                     /locus_tag="OO172_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5511424.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5472..6209
                     /gene="hisA"
                     /locus_tag="OO172_001168"
     CDS             5472..6209
                     /gene="hisA"
                     /locus_tag="OO172_001168"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5511425.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6191..6967
                     /gene="hisF"
                     /locus_tag="OO172_001169"
     CDS             6191..6967
                     /gene="hisF"
                     /locus_tag="OO172_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5511426.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6961..7572
                     /locus_tag="OO172_001170"
     CDS             6961..7572
                     /locus_tag="OO172_001170"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5511427.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7758..8735)
                     /gene="wzzB"
                     /locus_tag="OO172_001171"
     CDS             complement(7758..8735)
                     /gene="wzzB"
                     /locus_tag="OO172_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5511428.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8881..10047)
                     /gene="ugd"
                     /locus_tag="OO172_001172"
     CDS             complement(8881..10047)
                     /gene="ugd"
                     /locus_tag="OO172_001172"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR5511429.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEDYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10297..11703)
                     /gene="gndA"
                     /locus_tag="OO172_001173"
     CDS             complement(10297..11703)
                     /gene="gndA"
                     /locus_tag="OO172_001173"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5511430.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11812..12171)
                     /locus_tag="OO172_001174"
     CDS             complement(11812..12171)
                     /locus_tag="OO172_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511431.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12821..13384)
                     /locus_tag="OO172_001175"
     CDS             complement(12821..13384)
                     /locus_tag="OO172_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511432.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13592..14530)
                     /locus_tag="OO172_001176"
                     /pseudo
     CDS             complement(13592..14530)
                     /locus_tag="OO172_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14593..14967)
                     /locus_tag="OO172_001177"
     CDS             complement(14593..14967)
                     /locus_tag="OO172_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5511433.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFELLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(14971..16119)
                     /gene="wzy"
                     /locus_tag="OO172_001178"
     CDS             complement(14971..16119)
                     /gene="wzy"
                     /locus_tag="OO172_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5511434.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16139..17044)
                     /gene="rfbG"
                     /locus_tag="OO172_001179"
     CDS             complement(16139..17044)
                     /gene="rfbG"
                     /locus_tag="OO172_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5511435.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17037..17927)
                     /locus_tag="OO172_001180"
     CDS             complement(17037..17927)
                     /locus_tag="OO172_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5511436.1"
                     /translation="MNSNIYAVIVTYNPELKNLNELITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17957..19213)
                     /gene="wzx"
                     /locus_tag="OO172_001181"
     CDS             complement(17957..19213)
                     /gene="wzx"
                     /locus_tag="OO172_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5511437.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19229..19774)
                     /gene="rfbC"
                     /locus_tag="OO172_001182"
     CDS             complement(19229..19774)
                     /gene="rfbC"
                     /locus_tag="OO172_001182"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5511438.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19779..20657)
                     /gene="rfbA"
                     /locus_tag="OO172_001183"
     CDS             complement(19779..20657)
                     /gene="rfbA"
                     /locus_tag="OO172_001183"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5511439.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20716..21615)
                     /gene="rfbD"
                     /locus_tag="OO172_001184"
     CDS             complement(20716..21615)
                     /gene="rfbD"
                     /locus_tag="OO172_001184"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5511440.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21615..22700)
                     /gene="rfbB"
                     /locus_tag="OO172_001185"
     CDS             complement(21615..22700)
                     /gene="rfbB"
                     /locus_tag="OO172_001185"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5511441.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23073..23966)
                     /gene="galF"
                     /locus_tag="OO172_001186"
     CDS             complement(23073..23966)
                     /gene="galF"
                     /locus_tag="OO172_001186"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5511442.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24141..25535)
                     /gene="wcaM"
                     /locus_tag="OO172_001187"
     CDS             complement(24141..25535)
                     /gene="wcaM"
                     /locus_tag="OO172_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5511443.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25546..26766)
                     /gene="wcaL"
                     /locus_tag="OO172_001188"
     CDS             complement(25546..26766)
                     /gene="wcaL"
                     /locus_tag="OO172_001188"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5511444.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSYEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26763..28043)
                     /gene="wcaK"
                     /locus_tag="OO172_001189"
     CDS             complement(26763..28043)
                     /gene="wcaK"
                     /locus_tag="OO172_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5511445.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRSYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMIADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28223..29701)
                     /gene="wzxC"
                     /locus_tag="OO172_001190"
     CDS             complement(28223..29701)
                     /gene="wzxC"
                     /locus_tag="OO172_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5511446.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVSVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29703..31097)
                     /gene="wcaJ"
                     /locus_tag="OO172_001191"
     CDS             complement(29703..31097)
                     /gene="wcaJ"
                     /locus_tag="OO172_001191"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5511447.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDTKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31152..32522)
                     /gene="cpsG"
                     /locus_tag="OO172_001192"
                     /gene_synonym="manB"
     CDS             complement(31152..32522)
                     /gene="cpsG"
                     /locus_tag="OO172_001192"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5511448.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32627..34063)
                     /gene="cpsB"
                     /locus_tag="OO172_001193"
     CDS             complement(32627..34063)
                     /gene="cpsB"
                     /locus_tag="OO172_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5511449.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASSEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGHHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34066..35289)
                     /gene="wcaI"
                     /locus_tag="OO172_001194"
     CDS             complement(34066..35289)
                     /gene="wcaI"
                     /locus_tag="OO172_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5511450.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLLFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35286..35765)
                     /gene="gmm"
                     /locus_tag="OO172_001195"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35286..35765)
                     /gene="gmm"
                     /locus_tag="OO172_001195"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5511451.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35765..36733)
                     /gene="fcl"
                     /locus_tag="OO172_001196"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35765..36733)
                     /gene="fcl"
                     /locus_tag="OO172_001196"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5511452.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36736..37857)
                     /gene="gmd"
                     /locus_tag="OO172_001197"
     CDS             complement(36736..37857)
                     /gene="gmd"
                     /locus_tag="OO172_001197"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5511453.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(37883..38431)
                     /gene="wcaF"
                     /locus_tag="OO172_001198"
     CDS             complement(37883..38431)
                     /gene="wcaF"
                     /locus_tag="OO172_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5511454.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
BASE COUNT        10276 a         9614 c         8648 g         9895 t
ORIGIN      
        1 ggctttcatc attgttgccg acaaattctg acgcgcttcg ctgtagctaa ttgtacgcat
       61 gtaaatctcc tattttgtac agttcattgt acaatgatag gtgttaatta actatttatt
      121 aattagtttg tagatcaaga cattgccagc gagacgaaaa atcagacttt gctctttttg
      181 gtgctgtcaa gttagaggac agtcctctta gccccctcct ttccccgctc attcattaaa
      241 caaatccatc gccataaaat atataaaaaa gcccttgctt tctaacgtga aagtggttta
      301 ggttaaaaga catcagttga ataaacattc acagagattt ttatgacacg cgttcaattt
      361 aaacaccacc atcatcacca tcatcctgac tagtctttca ggcgatgtgt gctggaagac
      421 attcagatct tccagtggtg catgaacgca tgagaaagcc cccggaagat caccttccgg
      481 gggctttttt attgcgcggt tgttaacggt tcagacaggt ttaaagagga ataagaaaat
      541 gacagacaac actcgtttac gcatagctat gcagaaatcc ggccgtttaa gtgatgactc
      601 acgcgaatta ctggcgcgct gtggcattaa aatcaatctt cacacccagc gcctgatcgc
      661 gatggcagaa aacatgccga ttgatattct gcgcgtgcgt gacgatgaca ttcccggtct
      721 ggtaatggac ggcgtggtag accttgggat tatcggcgaa aacgtgctgg aagaagagct
      781 gcttaaccgc cgcgcccagg gtgaagatcc gcgctacttt accctgcgtc gtctggattt
      841 cggcggctgc cgcctttcgc tggcaacgcc ggttgatgaa gcctgggacg gtccgctctc
      901 cttaaacggt aaacgtatcg ccacctctta tcctcacctg ctcaagcgtt atctcgacca
      961 gaaaggtatc tcttttaaat cctgcttact gaacggttct gtcgaagttg cgccgcgtgc
     1021 cgggctggcg gatgcgattt gcgatctggt ttccaccggt gccacgctgg aagctaacgg
     1081 cctgcgcgaa gtcgaagtta tctaccgctc gaaagcctgc ctgatccagc gcgatggcga
     1141 aatggaagaa tctaaacagc aactgatcga caagctactg acccgtattc agggtgtgat
     1201 ccaggcgcgc gaatcaaaat acatcatgat gcacgcaccg accgaacgcc tagatgaagt
     1261 catcgccctg ctgccaggtg ccgaacgccc aaccattctg ccgctggcgg gtgatcaaca
     1321 gcgcgtagcg atgcacatgg tgagtagcga aaccctgttc tgggaaacga tggaaaaact
     1381 gaaagcactg ggtgccagtt caatcctggt cctgccgatt gagaagatga tggagtgatc
     1441 gccatgagct ttaacacaat cattgactgg aatagctgta ctgcggagca acaacgccag
     1501 ctgttaatgc gcccggcgat ctccgcttct gaaagcatta cccgcaccgt taacgatatt
     1561 ctcgataacg tgaaaacgcg tggcgatgag gccctgcgtg aatacagcgc gaagtttgat
     1621 aaaaccacgg ttaccgcgct gaaggtgtct gctgatgaga tcgccgccgc cagcgaacgc
     1681 ctgagcgacg agctaaaaca ggcgatggcg gtggcagtaa agagtattga aaccttccac
     1741 actgcgcaaa aactgccgcc ggtagatgta gaaacgcagc caggcgtgcg ttgccagcag
     1801 gtcacgcgcc cggtggcttc agtgggtttg tatattccgg gcggctccgc cccgctcttc
     1861 tcaacggtat taatgctggc aactccggcg cgtattgcgg gctgtaaaaa agtggtgctg
     1921 tgctcaccgc cgccgattgc cgatgaaatt ctttatgcgg cgcagctgtg cgatgtgcag
     1981 gacgtgttta acgtcggcgg cgcacaggcc attgccgcgc tggcgtttgg tacggaatct
     2041 gtgccgaaag tggacaaaat cttcgggccg ggtaacgcct ttgtcaccga agcaaaacgc
     2101 caggtaagcc agcgtctgga cggtgcggcg atcgatatgc ccgcaggccc gtcggaagtg
     2161 ctggtgattg ctgacagcgg cgctacgccg gattttgtgg cttctgattt gctttctcag
     2221 gctgaacacg gccctgactc acaggtaatt ttactgaccc ccgatgctga tatggcgcgt
     2281 cgcgttgccg aggccgtcga acgccaactg gcagaactgc cgcgtgccga aaccacccgc
     2341 caggcactga acgccagccg cctgatcgtg actaaagatt tagcgcagtg cgtggagatc
     2401 tccaaccagt acggcccgga gcacctgatc attcagaccc gcaacgcccg cgaactggtc
     2461 gatggcatca ccagcgccgg ttcggtattt cttggtgact ggtcaccgga atcggcaggc
     2521 gactacgcct ccggcaccaa ccacgtgctg ccgacttacg gttacaccgc cacctgttcc
     2581 agcctcgggc tggcagattt ccagaaacgc atgaccgtac aggaactgtc gaaagaaggt
     2641 ttctccgcgc tggcttcgac cattgaaaca ctggccgccg ccgagcgcct gaccgcccac
     2701 aaaaatgccg ttactttgcg tgttaacgtc cttaaggagc aagcatgagc accgtgacta
     2761 ttactgattt agcgcgtgaa aacgtccgca acctgacgcc gtatcagtcg gcgcgtcgtc
     2821 tgggcggtaa cggcgacgtc tggctgaacg ccaacgaata tcccacagcc gtggagttta
     2881 agcttactca gcaaacgctc aaccgctacc cggaatgcca gccaaaagcg gtgattgaaa
     2941 attacgcgca atatgcaggc gtaaaaccgg aacaggtgct ggtcagccgt ggtgcggacg
     3001 aaggtattga actgctgatc cgcgcttttt gcgaaccggg taaagacgcc atcctctact
     3061 gcccgccaac gtacggcatg tacagcgtca gcgctgaaac cattggtgtc gaatgccgca
     3121 cagtgccgac gctggaaaac tggcaactgg acttacaggg catttccgac aagctggacg
     3181 gcgtaaaagt ggtctatgtt tgcagcccca acaaccccac cgggcaactg atcaatccgc
     3241 aggattttcg caccctgctg gagctaaccc gcggtaaggc gattgtggtt gccgatgaag
     3301 cttatatcga gttttgcccg caggcatcgt tagctggctg gctggcggaa tatccgcacc
     3361 tggcgatttt gcgtaccctt tcgaaagcct tcgcgttggc ggggcttcgt tgcggattta
     3421 cgctggcaaa cgaagaagtc atcaacctgc tgatgaaagt gatccccccc tacccgctct
     3481 cgacgccggt tgccgacatt gccgcacagg cgttaagccc gcagggaatc gtcgccatgc
     3541 gcgaacgggt ggcgcaaatt attacagaac gcgaatacct gattgccgca ctgaaagaga
     3601 tcccctgcgt ggagcaggtt ttcgactctg aaaccaacta cattctggcg cgctttaaag
     3661 cctccagtgc agtgtttaaa tctttgtggg atcagggcat tatcttacgt gatcagaata
     3721 aacaaccctc tttaagcggc tgcctgcgaa ttaccgtcgg aacccgtgaa gaaagccagc
     3781 gcgtcattga cgccttacgt gcggagcaag tttgatgagt cagaagtatc tttttatcga
     3841 tcgcgatgga accctgatta gcgaaccgcc gagtgatttt caggtggacc gttttgacaa
     3901 actcgccttt gaaccgggcg tgatcccgga attgctgaag ctgcaaaaag agggctacaa
     3961 gctggtgatg atcactaatc aggatggtct gggaacacaa agtttcccgc aagcggattt
     4021 tgatggcccg cacaacctga tgatgcagat cttcacctcg caaggcgtgc agtttgatga
     4081 agtgctgatt tgtccgcacc tgcccgccga tgagtgcgac tgccgtaagc cgaaagtaaa
     4141 actggtggag ggttatctgg ctgagcaagc gatggatcgt gccaacagtt atgtaattgg
     4201 cgatcgcgcg accgacattc aactggcgga aaacatgggc attaatggtt tacgctacga
     4261 ccgcgaaacc ctgaactggc caatgattgg cgagcaactc actaaacgag accgttacgc
     4321 ccatgtagtg cgcaacacca aagagacgca gattgacgtc caggtgtggc tggatcgcga
     4381 aggtggcagc aagattaaca ccggcgttgg cttctttgat cacatgctgg atcagatcgc
     4441 cacccacggc ggtttccgca tggaaatcaa tgtcaaaggc gacctctata tcgacgatca
     4501 ccacaccgtc gaagataccg gcctggcgct gggcgaagcg ttaaaaattg ccctcggcga
     4561 caaacgcggt atttgccgct ttggttttgt gctgccgatg gacgaatgcc ttgcccgctg
     4621 cgcactggat atctctggtc gcccgcacct ggaatataaa gccgagttta cctaccagcg
     4681 tgtgggcgat ctcagcactg aaatgatcga acacttcttc cgttcgctct cttacaccat
     4741 gggcgtgaca ctacacctga aaaccaaagg taaaaacgat catcaccgtg tagaaagcct
     4801 gttcaaagcc tttggtcgta ccctgcgcca ggccatccgc gtcgaaggcg acaccctgcc
     4861 ctcgtcgaaa ggagtgctgt aatgaacgtg gtgatccttg atactggctg cgccaacctg
     4921 aactctgtga agtctgccat tgcgcgccac ggttatgaac ccaaagtcag ccgtgacccg
     4981 gacattgtgt tgctggccga taaactgttt ttacccggcg tcggcaccgc acaagcagcg
     5041 atggatcagg tgcgtgagcg cgagctgttt gatctcatca aagcctgtac ccaaccggtg
     5101 ctgggtatct gcttagggat gcaactcctg ggacgacgca gcgaagagag caatggcgtc
     5161 gacttgctgg gcatcatcga cgaagacgtg ccgaaaatga ccgactttgg tctgccactg
     5221 ccacatatgg gctggaaccg cgtttatccg caggcaggca accgcctgtt tcaggggatt
     5281 gaagacggcg cgtactttta ctttgttcac agctacgcaa tgccagtcaa tccgtggacc
     5341 atcgcccagt gtaattacgg cgaaccgttc accgcggcgg tacaaaaaga taacttctac
     5401 ggcgtgcagt tccacccgga gcgttctggt gccgctggcg ctaagttgct gaaaaacttc
     5461 ctggagatgt gatgattatt ccggcattag atttaatcga cggcaccgtg gtgcgtctcc
     5521 atcagggcga ttacggcaaa caacgcgatt acggtaacga cccgctgccg cgcttacagg
     5581 attacgctgc gcaaggtgcc gaagtgttgc acctggtgga tctgaccggg gcaaaagatc
     5641 cggctaagcg tcaaatcccg ctgattaaaa ccctggtcgc gggcgttaac gtcccggtgc
     5701 aggttggcgg cggtgttcgt agtgaagaag acgtggcggc gttactggaa gctggcgttg
     5761 cgcgcgtggt ggtcggttcc accgcagtga aatcaccgga cgtagtgaaa ggctggtttg
     5821 aacgtttcgg tgccgatgcg ctggtgctgg cactggatgt ccgtattgac gagcaaggca
     5881 acaagcaagt ggcagtcagc ggctggcaag agaactcggg catttcactg gaacaactgg
     5941 tggaaaccta tctgcccgtc ggcctgaaac atgtgttgtg taccgatatc tcgcgcgacg
     6001 gcacgctggc gggatctaac gtctctttat atgaagaagt gtgtgccaga tatccgcaag
     6061 tggcgtttca gtcctccggc ggcattggcg acattaacga tgttgctgcc ctacgtggca
     6121 ctggcgtacg cggcgtaata gttggtcgtg cattactgga aggtaaattc accgtgaagg
     6181 aggccatcgc atgctggcaa aacgcataat cccgtgtctc gacgttcgtg atggtcaggt
     6241 ggtgaaaggc gtacagtttc gcaaccatga aatcattggc gatatcgtgc cactggcaaa
     6301 acgctacgcc gaagaaggtg cagatgaact ggtgttctac gatatcaccg cttccagcga
     6361 tggccgtgtg gtagataaaa gctgggtatc tcgcgtggcg gaagtgatcg acattccgtt
     6421 ttgtgtggcg ggtgggatta agtctctgga agatgccgcg aaaattcttt cctttggcgc
     6481 ggataaaatt tccatcaact cccctgcgct ggcagaccca acattaatta ctcgcctggc
     6541 cgatcgcttt ggcgtgcagt gtattgtggt tggtattgat acctggtacg acgccgaaac
     6601 cggtaaatat catgtaaatc aatataccgg cgatgaaagc cgcacccgcg tcactcaatg
     6661 ggaaacgctc gactgggtag aggaggtgca aaaacgcggc gccggagaaa tcgtcctcaa
     6721 catgatgaat caggacggcg tgtgtaacgg ttacgacctc aagcagctga aaaaagtgcg
     6781 tgaagtttgc cacgtcccgc tgattgcctc cggtggcgcg ggcaccatgg aacacttcct
     6841 ggaagccttc cgcgatgccg acgttgacgg tgcgctggca gcttccgtat tccacaaaca
     6901 aataatcaat attggcgaat taaaagcgta cctggcaaca cagggcgtgg agatcaggat
     6961 atgttaacag aacaacaacg tcgcgaactg gactgggaaa aaaccgacgg tctgatgccg
     7021 gtgattgtgc aacacgcggt atctggcgaa gtgttaatgc tgggctacat gaacccggaa
     7081 gccttagaca aaaccatcga aagcggtaaa gtcaccttct tctcgcgcac taaacagcga
     7141 ctgtggacca aaggcgaaac ctcgggcaat tttctcaatg tggtaagtat tgccccggac
     7201 tgcgacaacg acacattact ggtgctggcg aatcccatcg ggccaacctg ccacaaaggc
     7261 accagtagct gcttcggcaa caccgctcac cagtggctgt tcctgtatca actggaacaa
     7321 ctgctcgccg aacgtaaata tgccgacccg gaaacctcct acaccgccaa actgtatgcc
     7381 agcggcacta aacgcattgc gcagaaagtg ggcgaagaag gcgtggaaac cgcactggca
     7441 gcaacggtac atgatcgctt tgagctgacc aacgaggcat ctgacttgat gtatcacctg
     7501 ctggtgctgt tgcaggatca ggatctggat ttaacgacag taattgagaa cctgcataaa
     7561 cggcatcagt gagttacggg ctgagcaggt gtgatgttgt tgtcacaccc ggagccattg
     7621 cccgatgcgc aaacttatcg tgcctacaca ttatctcaac acacgtaggc cggataagat
     7681 gcgccagcat cgcatccggc aaaaaaaacg ggcatggtgt caccaccctg cccgtttctc
     7741 ttaaatgcac aataatatta cttcgcgttg taattacgca gagcgttacg ccccaacaca
     7801 atccccgcgc caaccatgcc gcccagaagc actgccagaa tcaaggtgat tgcctttttc
     7861 ggactatcgc gacgaatagg taacgtcggt ttcatcacat agcggtaagc atgaatatca
     7921 agatcatcaa actttaattt ttcaatatcc aacaggtttt gacgtgtctg atagtagttt
     7981 ggtgagaaca ccaacggacg agtcgcttca tgcttaatca tcgactccag cgcttcgctc
     8041 cctagaagga acaacgtatc ttgcgtcaca tcttcagtct gctgaacctg tggctttgtc
     8101 acctgcgcct gattcgcata ctgcaacgct tcctgaatct gacggatacg cagatctttc
     8161 tgctcctgcg cgaccacttc ctgggttctt aaagagtcct gcaagttttt ccgtcccaga
     8221 gcaatgttgt ccttgagatc cttttctagc tcttgattca ctttatcatc aacttgctga
     8281 atgtattggg ccaacttcat ttgtgcgccc tctgcagttt gcccaacata agaaacagtc
     8341 aatggtaatt gttggttctt aacagaaggt tcgatggtaa gtttttctgg ctcttcctga
     8401 ttatccagcg tttctgctaa tgcagagaag gcagaactga agcgaccaat taacgtctcc
     8461 tgcaaatccg atactttcgg tgcagcctga ccatagataa cattcatggc attgttatag
     8521 ccagcaattt gccccacgtc gggctgagtg ataattgctg ttgacgtcca tttctccttc
     8581 gctactgcca aataaccaat agccagggca atagccacaa tgacggaaat gataattgtc
     8641 atcttgccac gccacaactg cactagtaaa tcaatcaaat caatctgttc cgggtcatgg
     8701 ttttgcccag aaacattatt attttctact ctcatcatta ccctaactga cgaaaatttc
     8761 caagtccgca tagtttagcg ataatccccg tgaatgctat aggataagtg ataaaaataa
     8821 tgaattattg cggaggtaac aaaagacaat cagggcgtaa atagccctga taacaagatg
     8881 ttagtcgctg ccaaagagat cgcgggtgta gaccttatct gccacatcct taagctcttc
     8941 tgccatacgg ttggagataa tgacgtcggc ttgttgtttg aaggtggcga gatcacgttc
     9001 caggcgagag ttgaagaatg agtcttcttt catcaccggc tcgtagatga tcacttcaac
     9061 gcctttcgcc ttgatacgct tcataatccc ctgaatagaa gacgtacgga agttatctga
     9121 cccgctcttc ataatcagac gataaatacc caccacttgc ggtttgcgtg acaaaatggc
     9181 atcggcaata aaatctttac gcgtgcggtt tgcatcgaca attgccgaga tcaggttatt
     9241 cggcacagac tggtagttcg ccagtaactg cttggtatct ttcggcagac aataaccacc
     9301 ataaccaaac gacggattgt tgtaatggtt gccaatacgc ggatcgagac aaacgccttc
     9361 gataatctgg cgagtattca gacctaaact ttctgcataa ctatccagtt cattaaagta
     9421 tgctacgcgc atcgccagat aggtattagc gaaaagttta atcgcttctg cttcagtgga
     9481 gtcggtaaac agggtcggga tattttgctt aatcgcgcct tcctgtaaca acgcagcaaa
     9541 acgttctgcg cgttctgaac gctcaccgat gacaatacgt gacggatgga gattatcgta
     9601 aagggcttta ccttcgcgga ggaattccgg agagaaaata atattttcag tacgatattt
     9661 cttatgcatc gctgcggtaa aaccaacggg aaccgttgat ttgatgacca taaccgcata
     9721 aggatttatc tcaactacgt ctttaattac tgattctaca ctggatgtat tgaaataatt
     9781 agttttagga tcatagtcgg ttggagtggc gatgatgaca taatcagcat cccggtagtc
     9841 ttcattttta tctaatgtgg cattaaagtg tattttatct gattgcaaaa actgctgaat
     9901 ttccttatca acaataggag atatccgatc attcagcata gcaacgcgtg acggtaaaat
     9961 atctaatgcc acaacctcat gattttgtgc gattagaagc ccgtttgaca agcctacata
    10021 gccagtaccg gaaatggtga ttttcatttc atgctctcag aattaactta actgtgaatc
    10081 atgatgtttt tagcatcgtg ataagatgct aaaagtttta acgctacgtt gtcagatggt
    10141 caacgcaacg aaaaacaatt cagattttct ttataagaat agctgatgtt tattaagtta
    10201 atattaagca gtgaaattta gtctataaga tatttggcaa aaaaaagccc ggtgcaatat
    10261 acgccgggcc tcaattttat tgttggttaa atcagattaa tccagccatt cagtatggaa
    10321 cacaccttct ttatcaatgc gcttataagt atgcgccccg aaatagtcac gctgtgcctg
    10381 gatcaggttc gcaggcagaa ccgcggcacg gtagctatcg taataggcaa ccgcagcggc
    10441 gaaggtcgga accgggatac cgttctgtac tgcataagcg acgacatcgc gcagcgcctg
    10501 ctggtagtca tcggcaattt gcttgaagta cggtgccagc agcaggttag cgatctgcgg
    10561 attttcggca taagcatcgg tgattttctg caggaactgc gcacggatga tgcagccagc
    10621 acggaaaatc ttcgcgattt cgccgtagtt cagatcccag ttgtattctt cagacgcagc
    10681 acgcagctga gagaagccct gagcgtaaga aacgattttg cccagataca gcgcactgcg
    10741 aactttttcg atgaactcag ccttgtcgcc tgctgactga gcttgcggac cagagagaac
    10801 tttagatgcg gcaacgcgct gatctttcag agaagagata taacgtgcaa acacagactc
    10861 ggtaatcagc gacagcggtt cgccgagatc cagcgcgctc tggctggtcc atttaccggt
    10921 gcctttgttt gctgcttcat ccagaatcac atcaaccagg tagttaccgt cttcatcttt
    10981 tttggtgaag atatctttag taatgtcgat caggtagctg ctaagttcac cgttattcca
    11041 ctcggtgaaa gtctgggcca gttcttcatt agatagattc aggccacctt tgagcagaga
    11101 atacgcttca gcaatgagct gcatatctcc gtattcaata ccattgtgaa ccatcttcac
    11161 ataatggcct gcgccgtcgg caccaatata agtaacgcat ggctcgccgt cttctgccac
    11221 ggcagcgatt ttagtcagga taggcgcaac cagctcataa gcctctttct gcccgccagg
    11281 cataatagaa ggacctttca gcgcgccctc ttcaccgccg gaaacaccgg taccgatgaa
    11341 gttaaagccc tctgctgaaa gctcacgatt acgacgaatg gtgtcctgga agaaggtatt
    11401 accaccatca atgatgatgt cacctttatc gaggtatggt ttcagggaat caatcgcagc
    11461 atccgtgcct gcacctgctt tcaccattaa caggatgcga cgaggcgttt ccagagattc
    11521 aacaaactct ttcaccgtat agtaaggagc cagtttcttg cctggatttt cagcaatcac
    11581 ttcttccgtc ttttcgcggg agcggttgaa aatagagacg gtataaccac gactttcgat
    11641 gttgagcgca aggttgcgcc ccatcactgc cataccgact acgccgatct gttgctttga
    11701 cattgtttac tcctgtcttc tatattatta accgtctatt aaccgttata ttattaaccg
    11761 tcgtgatttt catcttctaa tcaaggaata catatggtta cagcatgctt cttactttat
    11821 agcgataagt atgccattgg gtgtatccat aagaactttg aattttggat acttttctgg
    11881 attaaaatta gcatagcttc gtttagatat aacaataaac gaactattaa aagatttcat
    11941 cttctctatt aaatcatcct gtgggattac ctcacactcg ggattactaa tagtccaatc
    12001 actatatctt gctaatccaa agacgtcacc aattgccatc cctttataga agtaaatacc
    12061 atcctccaag cctaattgga taagattatc tccatataac tcactatatt cacttgcttt
    12121 cttcatcagt tcatagctat agcgactttc taagcgtgaa ttaaaattca agtattcaga
    12181 ataaaaatat ttgaattcaa aatgcaacga tcgccccgaa aaaaaaataa tacacacaag
    12241 gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta aacttgcaat
    12301 tgagatcgca accagcaata ctgacaatgg gtaaatgggt gataaatacc tgtcaccttg
    12361 cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata cccaaagtat
    12421 tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat cactgccaac
    12481 tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc gagcttgttc
    12541 tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata tatttcctcc
    12601 agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat aaatgccacc
    12661 aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt tcgctatcaa
    12721 tataacaaat atgatggctc catatatggc accaaatagt tttacgccac aagcaattcc
    12781 aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt ctgagcgaat
    12841 atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata tatgcataac
    12901 caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata atgctaggat
    12961 aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt acaaaaaaac
    13021 agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct aaagccatta
    13081 aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca acaattgcat
    13141 ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc caatcaccgg
    13201 gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat aaaataacaa
    13261 acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga cgtttaataa
    13321 tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta aattgcccga
    13381 ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat aggcaataaa
    13441 aaaataaaga aaatttgtta agtcctgcca ccccctcctt aaaaaaagat gacaatacaa
    13501 tattaccaat gacaaaaaat gtcattccaa aagcaaaaca atacacaaca tacaaataca
    13561 acggcaattc aatcatagta aaatactcta tttacattat cagtttatag tttttttcag
    13621 aaagataaaa taatgccttc atttcacgtc tagttgttgt atttgaatgc aaaataatgc
    13681 cacatacaaa tgccattatt gacgataaca tgatggaggc agttaaaaag gcagtcggaa
    13741 atcttctcac caaacctgta tcaatataat caacaatgat gggcaatcct aaaattatag
    13801 acaaagcagc aagtaaaacg ccgataatac taaaaaagta tagtggccgt tcaagcgagt
    13861 aaagtttaat tattgttttc agaatcttga acccatcact ccaagttgat agtttgctca
    13921 cactcccttc agatcgctcg ccatattttg tattaacctc accatacttc atacgtaatt
    13981 ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc ccttgataga catggaaaag
    14041 atttaacata gcgtctggaa aatacacgat accccgacaa catatcagta aaattccctt
    14101 taaagatagt ctgaacagta ccagtcaaca gtttatttcc tcagcgatgc ccttttctgt
    14161 atgtattttg atctccattc tcttgacgac atcctacaac catatccaat tgatttttta
    14221 tgagatggtt aatcatttct ggagcagaac ttgcatcata agtgccatct ccatcaacca
    14281 ttagataaat atccgcatct acatctgaga acatacgtct tacgacattc cccttgcctt
    14341 tcagaggaac agagtgaact gttgctccag catcctctgc cacttgtgct gtactgtcag
    14401 tcgaattatt atcaaataca tatatagaag cagttggaat attcgttttg aaatcattta
    14461 tgacttttgc tattgccttc tgctcattat aacaaggaat aattacagcg atattaaaag
    14521 agtgatacat aagcccctca tataaactat ttagctaatg tgcaagtaat tgtgttttta
    14581 acaaatcgtt tactaacgaa aaatgaaata ttttgatgcg caatatccta aaaccaaact
    14641 aaaaagagaa aaggtgaaca atgtaaatat tggagataaa gctaagagtt caaataaaac
    14701 gccgaataaa taactcatcg cccccataaa aaatataaat ataaaatatc tagccttggt
    14761 tgcagatact ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg ctgcacatat
    14821 aaaaccaaca atgttacttc ggccttgacc aaaagctaaa ttattataac acgcataaaa
    14881 taccccccaa tgtaaagcag tattgacaat tccaattaaa aaataactaa aaaaagaact
    14941 ggtcaataac ttccctattt ttaacatcct ttattttgct ccagaagtga ggttattact
    15001 aatttggata ttttctatag aaaataccca taaaactaca acgcctataa taaaaattcc
    15061 catgggtgta aaaagaaatg gattagttat agaaacggta atactgaaaa aaagtattaa
    15121 cgacatcatt aatgatgttt ttgtactatt taaaaaatag cctttcaaaa agataagcaa
    15181 cataggaact aatgatataa atacaccaat aacgcctgtt ttctgaagta tctcaagagg
    15241 tactatttca atccttaatc gatctaatat aaatgaacca aatcctcttc caaacaagaa
    15301 cgtcgctaaa tcaacatttt tataataaaa atataaatca ttaaatctaa cagagtcaga
    15361 atcttcactg cgtgatccga ccatgtaaag cataattata aatagaacgc tgcccaatat
    15421 tattataata gtatttaaat atagcttatg taatttaaaa ctggctaata acagtgataa
    15481 tgttgtaaaa agtaatacac ctcttgattc tgtcaataat atcgcaagca ctattatgag
    15541 acattttaaa tattttttgt cgacaaaact gataatcgca ccgacaccaa aaaagtagaa
    15601 ggatttggaa aaaaaagccc catcgggacg aaaaaagaac tcgctagtca atgataagtg
    15661 ttcataaatt aaattaaaat ttaacaaacc gaaattcaac aaaattatgt atgaaatata
    15721 taacagtgat ccatataatg caaatgcatt aactatttta ataaaaatct cacataaaaa
    15781 tacttttaaa gtatatctta gtgtaaatag atatataagt gacagcataa aaaggtatgg
    15841 ttgcacatca aacagtatat catttatttt attaccagct aataagccga caaagaagcc
    15901 ataaaaagga aataaaagca accatataaa aagactgaca catttttggg tgattgaaaa
    15961 attgaatgta actaaattaa aaattaaaag tataaaacta aatacaaata ataaatatcg
    16021 caaagggaat attcccaatg gctccagtaa tcttccacca ccaccaataa tcagttcaat
    16081 acataaaaat gttataaaaa ttttatttat attattcata ttcgtaaggt gatgtttttt
    16141 aaaatatgtt taaaatcaac attaataaaa tataaatttt atcagccttt ttccgcctta
    16201 acttccaaaa acttaaagcc aagatcaaat tgcctatata acctttaccc aattgattat
    16261 tcacaaagct atcatcagct aaggcattag ctattttagt tacttctttt cgataccact
    16321 tgtttctcac caaaccaagc cgtttgtatt ttgctttaaa actaatgttt gctcccactt
    16381 ggttgttttc atgctgtctg tacagcattg ttggttttct atcaatatac cacgaataat
    16441 ttctcgtccg cgcaaatgaa tataaaaacc aatcatgcag acagatatct tgtgcagcat
    16501 ttttattatt tatgatgaac ttcttaaatt caataagcgt ttcttttttt ataacatatg
    16561 tacaccccgg acctgctgct tcaaaaaaat aatcgaattg agtttgcgac tgagccttat
    16621 caactaaatt tttttgccct gatggataat atgcaattac attgctggaa taacaattcg
    16681 cattattaaa taccaagcag tcaattgcat tttttatttt atctttatac caaatatcgt
    16741 cctgatcact aaaacagata taatcatagt tttcaagatc gacatcttta attaatctat
    16801 aaaaattttt acctgccccc ccaaacttat tgacgcttgg aagtatattt ataattgagg
    16861 gatatgataa ttgaaattct tcacaaatat ttaatgtatt atcagttgat aaatcatctg
    16921 atataaaaat tgagatgtct acatcttttt gttcaataat actttttaat tgttcctcaa
    16981 tccagcattc gccattatac gtagccataa gtacagctac tcttttttga tcagaactat
    17041 gcattataac gaccgccccc agtaattcct cttattccat ccagcgtagc caaaatatgt
    17101 tttttcatat attcaatttt atttttttga gttgcgataa ttaaagtcgt atgaattaaa
    17161 gaaaaaacaa cttccctaat agagagtaaa agaggcacat gattttttct aagcaaaaga
    17221 aatgcatttc ttactcgata ataacgacgt actggcgagt gaattggtat gttaatccca
    17281 cagattttta ctgacttatc cccaatagca tgaaccattt ctatatcagg aatgatatga
    17341 actaatatgc catagtttaa gcatctaaga caccattctg tatcgacata atcaataaaa
    17401 agttcctcat ccatcattcc aacaatctca aaaacttctt ttgaaaccat ggttccggat
    17461 gagattgtta ctgaactttt aaacggtgtc tgtccttcag tgacatgaat tttctctcga
    17521 agaccatttt ttttgatatt acaaatagga taataatgtg acttatctct atcaataaaa
    17581 actgggccag ctatttttgc attttcatta tccatatagc aggaaagttt tttgataaac
    17641 tcattgcgaa tacggctatc ttgatcaaaa aaaattatct tctctgcccc tttttcaaga
    17701 gaatatcgaa tcccaatatt ttgtgctttt gctatacctt cattcctgcc aagacaaatt
    17761 aaatgaactt tttcaatgtc tgctaattta tctttcaagg tgaaattagt cctattatcg
    17821 actactacaa cgtaacaatt ttgctccttt agttcagtga tcagttcatt cagattttta
    17881 agttcgggat tatatgtcac aatgacagcg taaatattac tattcattat ccaagtgact
    17941 cagtaattgg ttaattttag ttttcatata cagaacacat tctagtataa agccattgta
    18001 ataacagagc atctaaaatt acacgtatgc tccaggatat tgcggcaccc agtaacccat
    18061 atttttcgac cataaaatac aaaagggcta aataaggaac caattcagca caatgaatca
    18121 aagcagtaat tttagatttt cctaatgcct ggattgcaga gaatggtatc attgccagcg
    18181 cattaaagaa aaaaccgatc aataacacat ttaatatatt aaaggaatta atttggtaat
    18241 tctcaccaaa ccatattttc aatacaagcc ctgaatataa caagcctatg attatcaccg
    18301 gcaaacagat tagaaacatt aaaagcaatg atttatttac attacgttta aattctttaa
    18361 agtctttgat gttacttaac cttggaaaca ctgccctccc gattgctgca ggtattattc
    18421 ctaattttag aataacctcc gctggtgcag aataaaatgc aactttatct gcccccataa
    18481 tatttgacac tataaatcga tcaaaataca ccatgaccgg acttatgata ttacttactg
    18541 tcatccaacc gccaaagaaa aagagtcgtt taaaggtttt ataacaaaaa tgaacccccg
    18601 aaattttaat atcattcctg acataatagg cagagactaa aatcgaaata actcttgcaa
    18661 atattaaacc agcaaccgct gccgacaatg tagcactata aaaaacaaat atggccggaa
    18721 ttcccgcgat gcaagagctt gatatagatt tttgaatatt tacaatgcca aatttttcat
    18781 ccccctcaag aatggctgac cataattgat ttagaataaa taatggaatg caaatagcca
    18841 acagtttaaa tgctagttgt atatcactat gttcaacacc agaaattttt agataattaa
    18901 caattccatc agagaaaatc aataataaaa aagcaccaaa gcatgaaaat agcaccaaaa
    18961 aagatgtact tgttgaaatt accttggttc tttcatgatg attatcacga tgaattgcaa
    19021 tttctctaat aacagagcga gtcaggccta catcaaaaat tccagcatac ccaacaagtg
    19081 caattgcaat tgtataaaca ccgaaacctt caggccccaa acctcgagcg agaaatccta
    19141 gcgaggggat agcaattaaa gttggtattg catagccaaa aaggttccag acactatttt
    19201 ttattatgct cattattaca tttctttatc atataaaata atcagcatct acaaattttt
    19261 gaccattaat atctttatca gaaagcaatg gattttgaac cggccattct atatttatac
    19321 ttttgtcgct ccataaaatc ccacgctctt gcatttttga atagaaatta tttgttttgt
    19381 agacaaattc tgcttcatca ctaagaacgc aaaacccatg agcgaatcct tccggtatcc
    19441 ataattgacg cttattctcc gccgacaaat ttacaccaac ccacttgcca aatgttggtg
    19501 atgatttccg aatatcaaca gctacgtcaa aaacttcacc gacaacacag cgtaccagtt
    19561 ttccttgagc ataaggttcc agctgatagt gcagtccgcg taacacaccc ttagttgatt
    19621 tagaatggtt atcctgaaca aactcaacct ttcgtcctac agcctcttcg aaaactttct
    19681 gattgaagct ttccataaaa aagccccgct catcaccaaa tacttttggt tcaaaaatta
    19741 gcacatcagg aatttcagtt ttaattacgt tcattttatt aataaccttt aatcattttc
    19801 agcagatact gaccataagc atttttcttc agcggctcgg ctaatacctt cacttgctca
    19861 gcatcaataa agcctttacg gtaagcaatt tcttccgggc aggaaacttt cagcccctga
    19921 cgctcttcaa ttgttgcaat gaagttgctt gcttcaataa gactttgatg tgtccccgtg
    19981 tccagccatg cataaccacg ccccatcata gcgacagaca aacgtccttg gtccatataa
    20041 atacggttaa tatcggtaat ttccagttcg ccacgtgcag atggtttaag gtttttcgcc
    20101 atttctacaa cgtcattgtc atagaaataa agcccagtaa ccgcatagtt actttttggt
    20161 tccagcggtt tttcttccag actaattgcc gtaccgttat catcaaattc caccacaccg
    20221 tagcgctcag gatcatttac gtgataggca aaaaccgttg cgccactctc tctgttaaca
    20281 gcggtatcca ttaatttcgg caggtcgtga ccatagaaga tattatcacc aagtaccagc
    20341 gcacaatcat caccacctat aaattcttca ccaatgataa atgcttgcgc cagaccatcc
    20401 ggactcggtt gtactttata ttgcagatta agcccccact ggcttccatc accaagcaat
    20461 tgttgaaaac gtggtgtatc ctgcggcgta ctgataatca gaatatctcg aatacccgcc
    20521 aacataagcg ttgaaagcgg ataataaatc atcggtttat cataaatcgg tagcagttgt
    20581 ttgctcacag ccattgtcac aggataaaga cgagtgccgg aaccaccagc cagaataata
    20641 cctttacgcg ttttcatttc actattcctt ttaattcatc tcgctctggc atcatgagcg
    20701 agatgcaata atttgttaaa ttgccgtggt cgtaaataat tcgttgagca tacgtttcac
    20761 gcccacctgc cagtcaggca agacaagcgc aaagttctgc tgaaattttt cggtattgag
    20821 gcgagaatta tggggacgac gtgctggtgt gggataggcc gttgtcggca cggcattaag
    20881 tttgttaaga gcaaggttga tccccgctct gcgcgcttct tcaaatacca gagcggcata
    20941 atcgtgccag gttgttgtac caccagcaac cagatggtac aaaccagcaa cttctggttt
    21001 atttgcggcc acgcgaatgg catgagcggt gcaatcagcc agtaattcag cccccgttgg
    21061 tgcgccgaat tgatcgttta ttacagccaa ctcttcgcgt tcttttgcca ggcgcaacat
    21121 cgttttggca aagttatttc ctttaccagc gtatacccag ctggtacgga aaataagatg
    21181 ctttgcgcaa tgttcttgta acgctttttc cccagctagc ttggtttcac cgtagacatt
    21241 tagcggtgcg gttgcatccg tttccagcca tggcgtgtca ccgttgcctg ggaaaacata
    21301 atcagtggag tagtgtataa cccaggctcc gacttcatta gctgcttttg caatcgcttc
    21361 gacgcatgtc gcgttaagta attgcgcgaa attcggttct gattccgctt tgtctactgc
    21421 agtgtgagca gcagcattaa caataacatc ggggcgaatt tttttgacgg tttcagccac
    21481 accttcgggg ttgctgaaat ctccacaata atcagtggag tgaacatcga gcgcaatcaa
    21541 gttacccaga ggagccagag cacgctgcag ttcccaaccc acctgccctg ttttgccgaa
    21601 aagcaggata ttcattactg acggccctca tagttttgtt caatccacga ctgataagca
    21661 ccgcttttca cattctcaac ccaatttgta ttagccagat accattccac cgttttacga
    21721 atcccgctct caaacgtttc ttgcggtttc cagcccaatt cgcggctaat tttatcggca
    21781 tcaatggcgt aacggcggtc atgccccggg cgatcggcaa cataagtaat ttgctcacga
    21841 taagatttct ctttcggtac aatctcatcc aacaaatcac aaatagtgag cactacgtcg
    21901 atgtttttct tttcgttatg tccaccaatg ttataagttt cacccgcttt accttcggtt
    21961 acgacggtat ataacgcacg agcatgatcc tctacataca accagtcgcg aatttgatcc
    22021 cctttaccat aaataggtaa tgccttacct tccagagcat tcagaataac caatggaata
    22081 agtttttccg ggaagtgata aggaccataa ttgttcgagc aattagtcac aatggttggt
    22141 aaaccataag tccgcttcca ggcacgtacc aaatgatcgc tggaagcttt agacgcagaa
    22201 tacgggctac ttggcgcgta agctgtcgtt tccgtaaata gcggtaacgc ttcgttatta
    22261 tttgcttcat ccggatgggg taagtcacca taaacttcat cagtagaaat atgatgaaag
    22321 cggaaacttt ttttcttttc atcattcaga gcagaccagt aattgcgcgc cgcttctaaa
    22381 agaacataag tccccacaat attggtttca ataaatgccg cagggcccgt tattgaacgg
    22441 tcaacgtggc tctctgccgc caggtgcatc accgcgtctg gctggtgctg tgcgaaaata
    22501 cgcgacatgg ctacagcatc gcagatatct gcatgctcaa aagcataacg ttcagaatca
    22561 gaaacatcag cgagtgattc taggttcccg gcgtatgtta atttatcgac attaacaaca
    22621 ctatcttgcg tattatttat tatgtgacga acaacagcag aaccaataaa ccctgcgcca
    22681 ccagtaacaa gtatcttcac ttttttattc catatagcta tagagcatgc tgtgaaatag
    22741 actgctccag atttgattaa tagaggtatt aatacacgct accgcccctg gcttaacagc
    22801 taccagtgca ctgcatgcat ctcaacgatg tggcgagcgt tagccactcg cgccaaatcc
    22861 gaaaaattca aacgttaatt gtcttaccaa acagccgtag aaacaaggaa aatcctggaa
    22921 aaatttgaat aatatgagct atgtaacttc ttgtttttat aggctttata aaaacggcaa
    22981 cggcaggaaa attcaatgag caaaacttat gccacgaatc ttcactgccg ttataatttt
    23041 cttatcaacc gttacatccg gtcagatttt cattattcgc ttaacagctt ctcaatacct
    23101 ttacggaact tcgccccttc tttcaggttg cgtaggccat acttcacaaa cgcctgcata
    23161 tagcccattt ttttgccgca gtcgtaactg tcgccggtca tcagcattgc atcaacggat
    23221 tgttttttcg ccagctcggc aatagcatca gtcagctgaa tacgtcccca tgcaccaggc
    23281 tgagtacgtt ccagttccgg ccaaatatcg gcagaaagca catagcgacc tacggccatg
    23341 atgtctgagt ccagcgtctg cggctgatcc ggtttttcga taaattcaac aatacggctg
    23401 actttaccct cacggtccag cggctcttta gtctggatga cggagtattc agagaggtca
    23461 cccggcatac gttttgccag cacctggctg cggcccgttt cgttgaaacg tgcaatcatg
    23521 gcagcaaggt tgtaacgtag cgggtcggcg ctggcatcgt cgatcacaac gtctggcagt
    23581 accacgacaa atgggttgtc accaatggca ggtcgcgcac acaaaatgga gtggcctaaa
    23641 cctaaaggtt cgccctgacg cacgttcata atggtcacgc ccggcggaca gatggactgt
    23701 acttccgcca gcagttgacg cttcacgcgc tgctcaagga gtgattctaa ctcataagag
    23761 gtgtcgaagt ggttttcgac cgcgttcttg gacgcgtgag ttaccaggag gatttctttg
    23821 atccctgcag ccacaatctc gtcaacaatg tactgaatca ttggtttgtc gacgattggt
    23881 agcatctctt tgggtatcgc cttagtggca ggcaacatat gcatcccgag acccgctaca
    23941 ggaataactg cttttaaatt cgtcattatt tcatccacct gtaaaatggt tgctgaatta
    24001 tagcttgttc gatttttttc gccagcatca attaccctga attgattact gaattacttg
    24061 tgatgttacg ccgcttcgtt gtggattgca gtagcattgt tcctaagtat ggctccattt
    24121 ttccaggaat ggtcgcaaat ctactccctc cgttccggca atctaaagtt aatcttttcc
    24181 acattaacaa tatggtgatt aatcctgtcg atatcgacgg agctttgccc tctttcattc
    24241 accgcatgaa catttgcaag agacagcagt gtttcttttt tcgccataaa gacgccgcga
    24301 acgtctttgc gcatgtcgaa gttcatgctc aatgctggtc caactgagga ttcctgcatc
    24361 acattgatat ttcgtataaa aagatgttgc ggcttgttgt gtaactccag cgaggcacgc
    24421 ttcatctcaa tgttagttag cgacacaaag gagacggcat tcccggcgga gatttggatg
    24481 ccgcgcaatt tataagcaag atgggtatta tccagttgaa tattattcac tcggaaattt
    24541 tgcggtatcg agagatattt gcctttaatt accccatagc cgattaacat cccggcgcta
    24601 ttaatcattt caatattatc aatcacgaaa ttgtcacaac cgtaaatagc gactgtcgcg
    24661 ttatcaatgc ccgctttctt actgaaatcc ggcgtgatat tgcgggcatt gatattacga
    24721 ataacaaaat gtttaccatt ttcaacatgg ttcaactgcc gacaatccga tcccgtgata
    24781 ttcgccacga caaagttttt cactgcctgg tcttccgggt agttgttatc ataagtgctt
    24841 cccgcaaggc ctatgccgat gccccagttg attttgccgt tggtacagtt gatacgctcg
    24901 atgacatggt cagatatcaa aatatcactg tcgttaattg ccacgttcca ttcaatggcg
    24961 tcgccctgta agtcgctgaa cttacagttg gtaatgttgg caccgataat ctggttatga
    25021 aatccctggc gtaagatggc gtaattagcg tggctgacag tgaggttatc gatggtcagg
    25081 ttgcgcatga cccgtttgtt tttgccgcca atataaatct gcattaccgg gccaaagccg
    25141 ctcatcgcca gccctttgat ggtgcagtca gaaccacgca catccagggt aatgttatgc
    25201 ataccgccgc cctctccccc tgtcacctgg ctgccgtcct gtaagacaaa tcgccctctg
    25261 ccgttgccgc gcaggcttcc aaggatgtgt aacgttttgc cgggagggat gaagatgccg
    25321 gtgttgatat tgtcacaaac gaatccggca ggcacgacga ccgtttgccc ttcgctgaag
    25381 gcttgtttaa atgaggcgat ccagtcatgt gggttgtagt cgttaatgtt aacgctttgt
    25441 cgggcgggaa gtgcgcgagc gaaaggggta tggaggaagg caagcgccga gcttgccgtc
    25501 aggaaggtgc gtcgggagag tgttttaaat ggcatacgtt ctcctctata aagcctgcag
    25561 caagctggcg agttctcgat taatcacctg ctggttaaaa tcgtgttcga ctttttcgcg
    25621 cgcacgtttg acgaccggag ccagttcgtc ggtatccagt tggctaaacg ccgccagacg
    25681 ttgtgccagt gcgcgagcat cgttctcagg caccagccag ccggatttat cggcctccac
    25741 cagttcagga attccgctat gcagagtaga aaccaccgga ataccgaccg ccatcgcttc
    25801 cattagcgcc accggaatgc cttccatatc gccatccgcg cccgttaccg atggcaacag
    25861 gaagacatcc gcgtcgtcga gcatctcctt cacttcatag ctcggtttaa agcccggcat
    25921 ctctaccaca tcttccagtt gatattgttc gatgagggtg cgcaggcgtc gttcccacag
    25981 gccaatgccg aggatgcgat agcgaaatgc cacgccctgc tctttcaact gacggcaggc
    26041 ttcaatcgcc acatgcaggc cttttttctc ggttaagcgt gcgacagata tgatttccag
    26101 cggcgttgcg ggcgctttca ccggacgcgg gctaaagcgc gtcatgtcca cgcccatacg
    26161 cgatacggcg attttttctc tggggcagcc cattttttgc agccttccgg cccacagatc
    26221 gcttatcggt aacatcaggt cgccacggcg aaacagttgt tgatattcgg gagtgtagtg
    26281 gttgagcact tcccgactgg agatatcaat accgtggaag atggtggcaa ttttgccgcg
    26341 aatgataccc agttcgcgta gttttgctgc ggttaccccc gccgggccaa aatgagcgat
    26401 gaacacatcg gcgcgaaacg gtgttgcgac ctgaccacaa atggcagaca aaatcaggtt
    26461 ccgcgactcg gcaccatagc gtttgaggtt gagcgcatgc caggtatttt tacgatgaat
    26521 gccacgtaag gtctggctgg cgcggtggcg cagtttcgct actttgcctg caggttcgtc
    26581 ctgtaaccag cgggttctgg cgacaaggtt gtatttcgtc catgccgcgt gggtgttctc
    26641 tgtatcgcct ttttgcaaag cgacaatctc cacttcaaag cccatatcaa taaacgcggt
    26701 aatttgattg aggacgaagg tttccgacga cagcgggaat ttcaataaga agaagccgac
    26761 cttcatttca cctccccgat gcgctcaagc acagactgca ccatctgcat tcccgtctga
    26821 cgttcgcgac tgacggcttc gttaaatcga gcgttcagca ccggaagctg gcctaaggta
    26881 tccgcaatca tcgcttgcag gctgccgtcc agtaaatgac ggatatcaat cgccatctct
    26941 ggcagtccca gctgctgcat aatcccggcg gatttatgtt catagttgat ggcaatcgcc
    27001 ggagtagcaa agttcattga gataatggcg gagtgcaggc gcgtaccgac ggtgagttca
    27061 caggctccga gaattttgcc catttccaga tcgttgagtt catccatcac tacgtggtaa
    27121 cgggcaggat cgctgatgtg ctggcgcaga ttgagcgcca ccatgcggtc gtctttgtta
    27181 tagctgtcga tacccgtaca ggtggagagc gcaatcacct gatagccttc atcgagaatg
    27241 cgattgacca ccccggcaaa ggctttttca tacgcttgtt gagtggtgcc gagacgtttg
    27301 tcgaacggtg ccagttcgcg cagggtaatt gccactgttt tctgttgtgc ggcaacgtcc
    27361 agccagtgtt gaacggcata gctggcggtg aagtcttcgg tgtggtgatc gaccagccac
    27421 gcggtatcga cgccatgttc cacttttgcg gtggtgatat tgctgcgttt catcaaatca
    27481 aggctgaccg attcgcgcag gatcagcgcg tcgcagtgac caaagacata gttcgccagt
    27541 tggttaaatt gctcatcctg gaaggggccg acgctgtgac caatcataaa cagcggcttt
    27601 ttcgccataa atgtgcaaag cgcatgttca aactgcggca cgccgtagag atcgacaaaa
    27661 aacgatccgc cgacctgaat aatggcgtcg taacctgaca gcaggcgcac gaagtcggtg
    27721 aatccctggg cgatggcgat attgcgcagc ttgccagtgt cggtgacgcg tgagagcaat
    27781 acctgatgct ggtagctgcg acggaggact tttttaacgc gcccgacaac gcccgccgcg
    27841 ctattgtgtt gtgtcatctg caggaacagc ggatcgccca ttaccgggcg gttgagcagc
    27901 caggaagaac tgaccggata gcggctcatc acgtccactt cggtatgtgg attgaggatg
    27961 ttgatggcat caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc gcaagtgtgg
    28021 ttgcccagaa taagtaattt cattttttcc tcataaattt gatgccaggt gaggctgtgt
    28081 ttgttgtgtt cgttgccgtt ttttgcctga tgcgacgctg gcgcgtctta tcaggcctac
    28141 aggtcccgag cacagaaccg taggacggat aaggcgtttt acgccgcatc cggcaaccgt
    28201 tgtcggaacc gaaaacagca attcaccccg cccgtaaaag cattttcatt ttttcgctgc
    28261 gacaaaactg acgcttcact tccaccacca gcggatggcg tgacagcaca atcatcacca
    28321 caaacgccag cacgcccgcg gctatttgca ccgccagcag catccccagc gccagttgcc
    28381 ctttcagcac aatgcccagc gcataactga ccaccagcgt cggcaacgag agataaaacg
    28441 gcagccacaa actcaggatg tactggcgat aactggaacc aagaaccggt ttaatcatca
    28501 cgaagtaact cagaatggtg ttgataatct gcaccagcag gaaaccgagc gtgacgccga
    28561 ttgcgcccgc catctgccca ccaataacaa tcgccggaat aaacagaaat gttttgaata
    28621 cattgaattt aaagctgata tcgacccgcg ctttcgccat cagcagcgaa ccaatcgggt
    28681 tacccacgga gcgcagcaga cccacaatac acagcaattg cagcaccgga ataatgctgt
    28741 tccacttttc gccgaatacc agcggtacga aattattcga caccaccatc agccccagca
    28801 gcgccggaaa gttgataatc cccactatcg acagcagctt gtagaagtta acacgcagct
    28861 tttcggtgtc gtcctgaatt ttggcaaacg ccggaaacaa cacgcgggtg atgattgggt
    28921 tcagcttcat cggcggtacg acggccacgt tgtacgccag gttgtatccc cctgccacgc
    28981 ccgcgccaag aatacgcgcc agcaccagcg ttgaaaggtt ggtgttgaga tagttgatga
    29041 tgctgtccgc cgtcagccag gcaccaaaac gtaagttcgg tgccacagac gccagcgaga
    29101 agtgcagacc ggggcaataa attttgcgtc caaagtagcc aaacagcagt gttctcaccg
    29161 cactattgac cagataaccg aggatcgcgg tcatcgccag cggccagaaa tgggcgctaa
    29221 ctaccgtaaa agtgaagccc gccagcaccg cgctggtttc gatcatgccg attttgttga
    29281 actccagctc cttctgcatc aacgcgcgga actgttgccc gtgggggatc accacaaacg
    29341 ccagcgacaa tgttttaatc aacggtgcca ggtccgggtt attcagcacg tcgccgatga
    29401 catcactcaa caaaaacacc gacacacata ccacaagccc cagcccgacg ttcagccagt
    29461 acagcgtggt gagttcaagg tgactgattt cttttcgctg aataatcgag ttagcgatac
    29521 cgaagtcaga aagcgtatct gccagcgcga taatcaccag cgacacagtc agcaggccga
    29581 actggtggtt gtcgataatc cgcgccagca cggtcatctg caccagcccg aggccgatga
    29641 tgatcaccgt ggcaatagcc gaccacttcg cgccgctgat ggttttttca cgtaagctca
    29701 tatcaatatg ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac gattttgata
    29761 tcgaaccaga cgctccattc gcggatgtac tcaaggtcga actcgacgcg tttttccatt
    29821 ttctccagcg tgtcggtttc gccgcgccag ccattaatct gcgcccagcc ggtaatgccc
    29881 ggtttcactt tatggcgcag catgtagcct tcaatgagct ggcgatactg ttcgttatgc
    29941 gctactgcgt gcggacgtgg accgacaatc gacatccccc cggtcagcac attgataaac
    30001 tgcggcaatt cgtccagcga ggtgcggcgc agaaagttcc ccactttagt gacgcgcgga
    30061 tcgttctgcg tcgcctgggt caccactttg tcgttctcca tcactttcat ggaacggaac
    30121 ttccacactt tgatcggctt gccatccata ccatagcgag tctggcggaa aataaccggc
    30181 cctggtgaac tgagtttcac cgccagcgca atacagcaca gcaccgggga gatcagcagc
    30241 agaataagcg tcgccagcac aatgtcttcc gcacgtttta gcaggcggtt accccccgaa
    30301 agcggcgtgt catacagcgg caccaccgga acgccgttca tctcttcgag acgtgaatgg
    30361 agaatgttga aggtaaagac gtcggggatc agcagcaccg aacaggtggt atccgccagt
    30421 tgatgggcca gttttttcac tcgtgcgccg tcgcacattt gcatcgcgat atagacgtta
    30481 tgaatcttgc ccgcttttgc atcctcgacc agctgttgca ggttacccgc ccagtcgtta
    30541 gaaacgccgc ccggtttcgt gtcgtggtaa acgcccacca cttcaaaccc taaccacggc
    30601 tgattacgga aactctccat cagcatttgt ccggcggcta aatcccccgc tacggcgacc
    30661 atccgcttgt tgtagccatg attacgcagc cagcccgccc caatgcgaat acatgaacgg
    30721 caaaccacca gtccgatgct ggtcagccca taccacgcca gccagatttt cagttgcgtg
    30781 tcgaaatcat tgttgaacgc caccagtccg gcgctgaaaa tcacgcttaa ggtccagttt
    30841 tgtagcaaca gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg ataaaaatcg
    30901 gtgatgccgc ccagcatctg gaacaccacc agcgtaatca gcgccaccaa caggtgcatg
    30961 tagaggaatg acagtccgct gacttcgcaa accagccata gcccggcaaa catgatggtg
    31021 atatctgaaa agcgttgcac catagagatt aacgatgcat tggttttcgc tcgctcgcgc
    31081 ttttttagat ttgtcatcgt tgttcctgtt attagcccct tacccgcagt ggggtaaggg
    31141 aagatccgac attactcgtt cagcaacgtc agcagagttc gcgttcgcgc ttccatcagc
    31201 ggcacatcac cgcgcgattc cacattcagg cgcaccaccg gttcggtatt ggaggtgcgc
    31261 aggttaaagc gccagtcggc aaaggtcatg ctgatgccat cgctgcgatc caccgccagc
    31321 gcctcacggc taaaatgctg ttccacgcgg ttaatcgcct caacgggttg cgccagtttg
    31381 ctgttgatct caccgcttgc cggaaacgcc gccatccggt cgcgcaccag ttcgcccagc
    31441 gtttttcctt tcaggcacac cagttcggcg accagcagcc acgggatcat gccgctgtcg
    31501 cagtaagcga aatcacggaa gtaatggtgg gcgctcattt cgccaccgta gatggcgtct
    31561 tccttgcgca tccgttcttt aataaaggcg tgtccggttt tcgacattac cggcgtgccg
    31621 cctgcagcag tcaccacatc aatagtgttc caggagagac gcggatcatg gatgatcttc
    31681 gcgccgggat ttttttcgag gaacgcttct gccagcaggc cgacaatgta gtagccctcg
    31741 ataaactgcc ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc aaaggcaatg
    31801 cccatatccg cgccgtgttt gatgaccgca ttacgggtgt cgtcgcgaca ttccggcagc
    31861 agcgggttag gaataccgtt ggggaaattg ccgtccggtg tgttgtgtac tttgattaat
    31921 tccacgggcg cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac cggacccgct
    31981 gcgccattcc cggagttgat caccagcttg agcggcgtga gatttttgac attgatataa
    32041 ccgaacaggt gatcaacgta agcgtcacgc aggttgattt gctgatagcg accgcgtttg
    32101 gtttcatcga cgggaggaaa gtcgttggct tcagccagac gctggacgtc gcgcagtccg
    32161 gtatcaccgc tgatcgggcg agccccctcg cgcaccagct tcatgccgtt gtaatccatc
    32221 ggattatggc tggcagtaac ttcaatgccg ccatccacgc cgagatggaa cgtggcgaaa
    32281 tagatctctt cggtgccgga cataccgata tccagcacat cgacgcccgc atcctgtaaa
    32341 ccttttgcca gcgccagttt taaggtttcg ctggtgagac ggacatcacc gcctaacaca
    32401 atggttttcg gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc gatatcttca
    32461 ttcagttctt cgcctaattt cccgcgaata tcataggctt taaagcaggt taattttttc
    32521 atgtcgttac cctttttcag gcaatagttg gccctgaccg aaacggccaa ttttattttt
    32581 gtcgttattt acaccgcggt ttcgcattca ttgcctgatg cgacgtttac acccgtccgt
    32641 agcgatccgc gaagcgcacc acatcatcct cttcgagata agagccggag cgcacttcaa
    32701 ttaaatcgag cggaattttc cccgggtttt ccaggcagtg cgtcgccccc agcggaatat
    32761 aaatggactc gttttcacca agcagtttga tatcaccatc aatggtgact tttgccgttc
    32821 ccgcgaccac tacccagtgt tccgcgcggt gatggtgcat ctgtaccgac aaaccctcgc
    32881 ccggtttcac ggtgatgcgt ttcacctggt agcggtcgcc cgcgtcgata gagtcatatt
    32941 tgccccacgg acgatacact tcgcgatgca cccgatgctc atggtgacca tcggctttga
    33001 tctgctcgac cacttttttc acatcctgta ccgcgttacg gtcggcaatc agcaccgcat
    33061 ctttggtctg caccactacc agatctttca ccccgacggt ggtgactagg cctgattcgg
    33121 catacacata gctgttttca gttttgtgat taatcacatc gccgtggcaa acgttgccct
    33181 cggcggtgtg ggcgctgatc tcccataatg aagaccagga accaacatcg ctccagcccg
    33241 catccatcgg caccacaaca gcatctgccg tacgttccat gaccgcgtaa tccaccgact
    33301 cttccgggca ggcgagaaac gcttcttcat ccacacgaat aaaatcgaga tccggatcga
    33361 cggcgctcat cgctttttcg caggcatcga ggatatccgg gcgatatttt ttcagttctt
    33421 cgagatagcg tccggcgcgg aacaggaaca tgccgctgtt ccagtaatat tcgctgcttg
    33481 ctacataggc ctgggcggtt tccagattcg gtttttcgac aaactgcgcc acttcaaagg
    33541 ccaccgtatc ctgctcaccc gcagacactt caccgcgacg aatatagcca taaccggttt
    33601 ctggtagatc cggcacaatg ccaaaggtca ccagcttgcc cgcttcggca taaggcatgg
    33661 cattacgcac ggcggcacgg aacgcgtctt tatcggcaat cacatgatcc gccgccaata
    33721 ccagcattaa cgggtcgctc tccgggctat gacgttttgc cgccagcgcc gccagtgcaa
    33781 tggcaggtgc agtgttacgc cctgccggtt cgagaataat gttctcggtg agtttgttca
    33841 gttgacgcag ctgttccgcg acaataaagc ggtgctgctc attgcaaatc accaccgggc
    33901 tttcgcactc cacgccgttc aggcggcaga tggtggtttg cagcatggtg agatcgcctt
    33961 tcaggcataa aaactgcttg gggtaaagta cgcgggaaag cggccataaa cggctaccgg
    34021 agccacctgc catcacaact ggatagagtt tcgactgcgc cataattatc cccgaatatc
    34081 atttataaat tgacgtaaca cgttctcttt atcgagcgtg cgttcggcat attcacgtgc
    34141 caccgtgtta tgtttgggca gcaggagcgc ctgacgaatc cccgccacca gcgcctcgac
    34201 cgattccggt tctacgcaaa ccgcaatacc cggaaaggtt tcgcaaagct gccccagttc
    34261 tgtgtgggcc tctgcggtaa tcaccgcgtt accgcctacc gccagaatat tggtcagttt
    34321 cgacggcaat acggcatctg ccgcgccgcg tttttgcacc accagatggc aatcgcccat
    34381 cttcagcagt gcgggtaaag cgtcatacga ttgcagcggg aaaaattgca tgttgcgcag
    34441 tccacgctgc tgcgccattt tttccagccg cgctttgccg ccgccctgcc cgacaatggc
    34501 aaaaagcagc ggttcatcgc gcaggagatc ggcagcttca ataacgtttt ccagcccctg
    34561 cttttcacca atattgccgg agtaaagaat gatttttttg ttatccggca ggccaagctg
    34621 gttacgaagg gcatcaacat cggcgtctgc aacatgctga aaacgggcaa tttccgacca
    34681 gttggggaag aagatgacgt tatccgccgc cacgcctttt tcgatggctt tattcatcat
    34741 cgaacgcgaa atcgtggaaa cgttatcgac gttatgcagt ccgctacgtt cgaacgccgt
    34801 tgccagctgt gccactttgc cgcctttgcc ttttccggca aggcccagcc ccagcatggc
    34861 gtccacttcg taatcctgaa tatgcagcac ggtacgcgca ccagagagct tcgccagcag
    34921 gcgcattccc ggcgtgcaaa acagcgttgg cactacgcca ataatgcgat ccggcttcca
    34981 gcgacgttgc gccatcagtg gaaagaaact gctgacggca aaactgccga gatgcaacaa
    35041 gcgtttcagg gtgctcggct gttttggcac gtacagcggg cagcgccaca ccgtggcggc
    35101 cccttcttct cgtttgtagc gccaggcgga atagttctca cccacctgcc attgcgggta
    35161 gtaaggcggt gcggtaatga cccgcacctc atgaccttgt gccgccaacc attccaccat
    35221 ctcgccggtg tatttgccga tgccggttaa ctccggcgag tagttaatgc cgtagaccag
    35281 tattttcata atccgggtac tccggcacgc ttctcagcga gaaaataggc gcggctgtta
    35341 gcgtgaacat cgttactggc gagcagcgcg tccggcgtca gccagcggta atcgtcatgc
    35401 tgctcatccg gcagtaacag ctcttcttcc gctactctga agcgaaaacc gagcaccaca
    35461 tagtgagtgg tgaaatccgt gccggagaag ttatcgtcat aaaaatgctg ccagacaccg
    35521 taaaactggc ctgctgttat cggcagacgc agccccagtt ccgccatcgt cagccgctca
    35581 aatgcggctt ccagcgtttc gtctttctgc acgcgccctc ccggcacaaa ccagtaaccc
    35641 tgcgccgggc ggttggttct tttgccaagc agaaactcgc cgcgactgtt ctcgacaata
    35701 aagtcgagag agacaagcgg tgtggagcgc actacagtgg caaagtcttc ctgacgtaaa
    35761 aacatcattc cccccgaaag cggtcttgat tctcaaggaa ccactggtaa gtgctggcaa
    35821 gccccgcttc cagtgagatt tcgtgatacc agccaagctg atgcaggcgc gtcacatcca
    35881 gcagtttgcg cggcgtacca tccggtttgc tggcatcaaa aaccacccga cctttgtaac
    35941 ccaccacttt ggcgatggtt tgcgccagct cgcggatagt gcagtcaacg cccgtgccga
    36001 cgttaatgtg cgacagcatc ggttgggtat tctccagcca gacttcatgc gccagctcca
    36061 tgacatgaat gctcgccgcc gccatatcat cgacgtgcag gaattcacgc atcggtgtac
    36121 cgctgcccca taccaccacg tccggcgcat tctgtgccgt cgcctcgtgg aagcgacgca
    36181 gcaatgctgg tatcacatgg gagttactcg ggtggaagtt gtcatgcggc ccgtacaggt
    36241 tggtcggcat gacagagcgg taatcgcgtc catactggcg gttgtaagat tcgcacagtt
    36301 tgatcccggc gattttggca atggcgtaag gctcgttggt cggctccagc gtgccctgca
    36361 ataactcgct ttctgccatc ggctgttttg ccagtttcgg gtagatgcag gacgatccga
    36421 gaaacatcag tttgttcacg tcgttctgat gcgcggcgtg aatgatgttg ctctcaatca
    36481 tcatgttctg gtagatgaaa tccgccggat aggtgttgtt ggcaacaatg ccgcccactt
    36541 tcgccgccgc cagatagacc tggtcaatgc gttcgctggc aaagaaatca tgcacggcgc
    36601 ggctgtccag caggttcagt tcatcgcggg tacgtaatac cagctccaca tcaccgcgct
    36661 gttcgagctg ccgcttgatg gcagaaccga ccatcccaca atggccagcg ataaaaatgc
    36721 gttgtttact tatgcttatg actccagcgc gatcgccacg tcgtagccgt gagatttcag
    36781 cagagagtgt tttttcgccg cttcgaggtc attagccacc atttcagaca ccatctctct
    36841 gagggtgatt tccggtttcc agcccagttt ttcgtgcgct ttggtcgggt cgccgagcag
    36901 cgtttcaact tctgccggac ggaagtaacg cggatcaacg gcgataatca catcacccgg
    36961 tttaacgccc ggcgcgtcat gcccggtgac ggaaaccaca atgcccttct cttcaacacc
    37021 cgtgccttca aagcgcagtt tgatgcccag ctgtgctgcc gccatttcca caaactgacg
    37081 tacggagtac tgaacgccgg tcgcgataac gaaatcttcc ggctgttcct gttgcagcat
    37141 catccactgc atttttacgt agtctttggc gtggccccag tcacgcaggg aatccatatt
    37201 gccgaggtac aggcacgact ccagcccctg ggcgatgttg gcgattgcgc gggtgatttt
    37261 gcgggtaacg aaggtttcgc cgcggcgcgg ggattcatgg ttgaagagaa ttccgttaca
    37321 agcgtacatg ccgtaggatt cgcggtagtt aacggtgatc cagtaggcgt acagtttggc
    37381 gaccgcatac ggagatcgcg ggtagaacgg cgtggtttct ttctgcggaa tttcctgcac
    37441 cagaccatac agttcagagg tggaagcctg atagaaacga gttttctttt ccagaccgag
    37501 gaagcggatc gcctccagca ggcgcagcgt acccatcgcg tcgacgtcag cggtatattc
    37561 tggtgactca aaagagaccg caacgtggct cattgcgccc aggttgtaca cttcatccgg
    37621 ctgtacttca cgcaaaatgc gcgtcaggtt agaggtatca ctcaggtcgc cataatgcag
    37681 atggaatttc gggttgcagg tgtgcggatc ctgataaatg tgatccacgc gctcggtgtt
    37741 gaatgacgat gcgcgacgct taataccatg cacctcgtaa cctttttcca gcagaaactc
    37801 tgccaggtaa gaaccgtctt gtccggttac accggtgatg agagcgactt ttgacatgtc
    37861 ttattcctct gtatttttga atttattcag tttcaacgcg ttcgcgtatc accactgcgg
    37921 gattcccccg gcaaaccaca tttgccggaa gcgatttaaa aacactgctt cgtgcaccca
    37981 cgacggtgcc gtcgccgatt gtgacgcctg gggcaacaaa gacatcggtt gccagccagc
    38041 atttctcgcc aatcacaata ggcgtggcgt taatggtgaa atgttgactt gcatggtcgt
    38101 ggctaccggt gcataaataa cttttttgcg atatcaccga atgtgcgcca atggttattt
    38161 caccgagggt atataaattg acgtcatcgc caacccacgc gtaatcacct aaggttaatt
    38221 tccacggata ggtaattttt actgacggac gaataactac gttttttcct atttttgctc
    38281 cgaataaacg taataaaaaa gcccgccagc gatacaatac ttgtggcgac caggcaaata
    38341 ttgttgcctg tactgcccac cataattgca ctttaatagc gttgccgccc cggaaccctt
    38401 tcggcaccga gaaaccgctt aaatcttgca ttg
//
LOCUS       DAJTNM010000020        37630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTNM010000020 DAJTNM010000000
VERSION     DAJTNM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.020"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..171)
                     /locus_tag="OO172_001199"
     CDS             complement(<1..171)
                     /locus_tag="OO172_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5511455.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQD"
     gene            complement(255..1139)
                     /gene="yhdJ"
                     /locus_tag="OO172_001200"
     CDS             complement(255..1139)
                     /gene="yhdJ"
                     /locus_tag="OO172_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5511456.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1225..1521)
                     /gene="fis"
                     /locus_tag="OO172_001201"
     CDS             complement(1225..1521)
                     /gene="fis"
                     /locus_tag="OO172_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5511457.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1547..2512)
                     /gene="dusB"
                     /locus_tag="OO172_001202"
     CDS             complement(1547..2512)
                     /gene="dusB"
                     /locus_tag="OO172_001202"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5511458.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2841..3722)
                     /gene="prmA"
                     /locus_tag="OO172_001203"
     CDS             complement(2841..3722)
                     /gene="prmA"
                     /locus_tag="OO172_001203"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5511459.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3734..5185)
                     /gene="panF"
                     /locus_tag="OO172_001204"
     CDS             complement(3734..5185)
                     /gene="panF"
                     /locus_tag="OO172_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5511460.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5175..5417)
                     /locus_tag="OO172_001205"
     CDS             complement(5175..5417)
                     /locus_tag="OO172_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5511461.1"
                     /translation="MDTSFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5526..6875)
                     /gene="accC"
                     /locus_tag="OO172_001206"
     CDS             complement(5526..6875)
                     /gene="accC"
                     /locus_tag="OO172_001206"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5511462.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHIEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6886..7356)
                     /gene="accB"
                     /locus_tag="OO172_001207"
                     /gene_synonym="fabE"
     CDS             complement(6886..7356)
                     /gene="accB"
                     /locus_tag="OO172_001207"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5511463.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8334..9308)
                     /locus_tag="OO172_001208"
     CDS             complement(8334..9308)
                     /locus_tag="OO172_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5511464.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9460..11400
                     /gene="csrD"
                     /locus_tag="OO172_001209"
     CDS             9460..11400
                     /gene="csrD"
                     /locus_tag="OO172_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5511465.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11705..12748
                     /gene="mreB"
                     /locus_tag="OO172_001210"
     CDS             11705..12748
                     /gene="mreB"
                     /locus_tag="OO172_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5511466.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12814..13917
                     /gene="mreC"
                     /locus_tag="OO172_001211"
     CDS             12814..13917
                     /gene="mreC"
                     /locus_tag="OO172_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5511467.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13917..14405
                     /gene="mreD"
                     /locus_tag="OO172_001212"
     CDS             13917..14405
                     /gene="mreD"
                     /locus_tag="OO172_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5511468.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14414..15007
                     /gene="yhdE"
                     /locus_tag="OO172_001213"
     CDS             14414..15007
                     /gene="yhdE"
                     /locus_tag="OO172_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5511469.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14997..16466
                     /gene="rng"
                     /locus_tag="OO172_001214"
     CDS             14997..16466
                     /gene="rng"
                     /locus_tag="OO172_001214"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5511470.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16534..20334
                     /gene="yhdP"
                     /locus_tag="OO172_001215"
     CDS             16534..20334
                     /gene="yhdP"
                     /locus_tag="OO172_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5511471.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVDGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20490..21935
                     /gene="tldD"
                     /locus_tag="OO172_001216"
     CDS             20490..21935
                     /gene="tldD"
                     /locus_tag="OO172_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5511472.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFSYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22063..22992)
                     /gene="aaeR"
                     /locus_tag="OO172_001217"
     CDS             complement(22063..22992)
                     /gene="aaeR"
                     /locus_tag="OO172_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5511473.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23175..23378
                     /locus_tag="OO172_001218"
     CDS             23175..23378
                     /locus_tag="OO172_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5511474.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23386..24318
                     /gene="aaeA"
                     /locus_tag="OO172_001219"
     CDS             23386..24318
                     /gene="aaeA"
                     /locus_tag="OO172_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5511475.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24324..26291
                     /gene="aaeB"
                     /locus_tag="OO172_001220"
     CDS             24324..26291
                     /gene="aaeB"
                     /locus_tag="OO172_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5511476.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26383..26655
                     /locus_tag="OO172_001221"
     CDS             26383..26655
                     /locus_tag="OO172_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5511477.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTLGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26711..26974)
                     /gene="yhcN"
                     /locus_tag="OO172_001222"
     CDS             complement(26711..26974)
                     /gene="yhcN"
                     /locus_tag="OO172_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5511478.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27339..27809)
                     /gene="argR"
                     /locus_tag="OO172_001223"
     CDS             complement(27339..27809)
                     /gene="argR"
                     /locus_tag="OO172_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5511479.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28244..29182
                     /gene="mdh"
                     /locus_tag="OO172_001224"
     CDS             28244..29182
                     /gene="mdh"
                     /locus_tag="OO172_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5511480.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29244..30311)
                     /gene="degS"
                     /locus_tag="OO172_001225"
     CDS             complement(29244..30311)
                     /gene="degS"
                     /locus_tag="OO172_001225"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5511481.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30401..31768)
                     /gene="degQ"
                     /locus_tag="OO172_001226"
     CDS             complement(30401..31768)
                     /gene="degQ"
                     /locus_tag="OO172_001226"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR5511482.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDEKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTNTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31922..32320)
                     /locus_tag="OO172_001227"
     CDS             complement(31922..32320)
                     /locus_tag="OO172_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5511483.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32514..33641
                     /gene="zapE"
                     /locus_tag="OO172_001228"
     CDS             32514..33641
                     /gene="zapE"
                     /locus_tag="OO172_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5511484.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33860..34288
                     /gene="rplM"
                     /locus_tag="OO172_001229"
     CDS             33860..34288
                     /gene="rplM"
                     /locus_tag="OO172_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5511485.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34304..34696
                     /gene="rpsI"
                     /locus_tag="OO172_001230"
     CDS             34304..34696
                     /gene="rpsI"
                     /locus_tag="OO172_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5511486.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35091..35729
                     /gene="sspA"
                     /locus_tag="OO172_001231"
     CDS             35091..35729
                     /gene="sspA"
                     /locus_tag="OO172_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5511487.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35735..36232
                     /gene="sspB"
                     /locus_tag="OO172_001232"
     CDS             35735..36232
                     /gene="sspB"
                     /locus_tag="OO172_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5511488.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36275..37630)
                     /gene="dcuC"
                     /locus_tag="OO172_001233"
     CDS             complement(36275..37630)
                     /gene="dcuC"
                     /locus_tag="OO172_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5511489.1"
                     /translation="MISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGFGGVL
                     DPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSLLSRP
                     LKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVIA
                     TTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDKK
                     DKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVVMLLS
                     LTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDA
                     VIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVM
                     IMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLF
                     Y"
BASE COUNT         9111 a         9411 c         9902 g         9206 t
ORIGIN      
        1 gtcctgaaaa ttcagttcgt cacagttaac cagttttagt ggattaactg agttcatatt
       61 acggcatttg tctgtttctg ttgtcagcag ttcaatccac tgcatcagta aagtatcaaa
      121 cagaaaaacg aagacagcga aaacgacgag ccaccaatac ttgcgaatca ttacctgagc
      181 ctgtgcatgt ttaaccaagt ctggcaggaa atatacacga aatgaataat ctgaaaacca
      241 ggcttacact tcctttactt tgcaatgaga tcggggtcaa cttcgcttaa ccggcttcgt
      301 tttgacaggt tgccggtctt tctttttttc actttcgcca gttcttccgc ggagtaatgc
      361 gacgcgacat ccagccgtcg aagccccatt ttgatgtact cgctgttgat ctcaatacca
      421 atgaattttc gtccgctggc gatggctacg gcaccggtag taaagctacc ggcaaacggg
      481 tcgagaacga tatcgcctgg gttggaagag gcgagaataa tgcgtttcag taaggctttc
      541 ggtttttgcg tcgggtggtt ctcatattca tccatcaaat aacgcacgcg tggaaaatcc
      601 cagacgttac ccggtacttt ttgatgattg tatggctgtg gaggattttt gcgataatag
      661 atcaacgcgc gctgcgatcc ggttttggct tctaccagaa tagcatcacc gttgaatgtg
      721 tagttctttg cgtctttcac catcatcagg atgggttcgt acatggagcc gtagtgtttt
      781 ttcgcctgta ctccagaact gtcatatgac cagacgatgc gacttttgat ggtaaaaagc
      841 ttgcggcact ggagatcgat aaagggcatg ttttccgtac tgttcataat gtacatgctg
      901 ccctgctttt tcagaacgcg gtggcactct gcaatcactt caaacagcca gtcgataaac
      961 agatcttctt tccaggcttc gatcaaacca tcaaaatttt taccgatgtt atacggtggg
     1021 tcggcaaaga gcagatcgac gctttcagta ggtagctttt taagttcggc aagggcatca
     1081 ccgtgaataa tggtcttagc ttcattacca aaccgggtcg gttcacatcc tgttctcatg
     1141 gtcactccct ttgtgacacc tataaaaaag gcgcttccct atgccgagta gcgccttttt
     1201 aatcaagcat ttagctaacc tgaattagtt catgccgtat tttttcaatt ttttacgcag
     1261 cgtaccacgg ttgatgccca tcatcagcgc agcacgggtc tggttaccac gggtgtattg
     1321 catcaccatg tccaacaggg gctgttctac ttcagccagt accagctcat agaggtcatt
     1381 cacatcctga ccattcagtt gagcaaaata gttcttcagt gcctgtttaa ccgagtcacg
     1441 caggggtttt tgggttacct gatcctgaga gttaacggta gaaacggtca gtacgtcaga
     1501 atttacgcgt tgttcgaaca tagttctgtc agctctttat ttctgtttac gcaaaatttt
     1561 cgaagtatgc ctccaacgcc tccagctgtt cgctggcatc ctcaatggcg ttgaatgtgc
     1621 gccgaaactg gtcatttgga gcgtgttcct ggagatacca ggaaacgtgt ttacgtgcaa
     1681 ttcggtaccc ttttgccgga ccataaaagt catgcagttc ccgaacgtgc gcgcaaagca
     1741 agcgcttaac ctctgccaaa ggcagcgggg gcagcaactc cccagtgtcc agataatgct
     1801 ggatttcccg aaagatccag ggtcttccct gagctgcgcg gcctatcatc agggcatccg
     1861 cccctgtata gtcgagcaca gctctggctt taagcgggtc agtaatgtcg ccattcgcga
     1921 taaccggaat ggaaactttc tgcttaactg cccgaatact gtcgtactca gcttctccat
     1981 tgaacaaaca ggcgcgtgta cggccatgaa tggtcagagc ctgaatgcca cagtcttcag
     2041 ccagttgggc aatctcttcg cagttacggt gttccggtgc ccagccggtg cgaatcttca
     2101 gggtaacagg aacgtccact gcattgacga cctcggtaag gatcgattta acgacatccg
     2161 ggtactgcaa gagggctgaa cctgcgagct tgcgattcac ttttttagcc gggcaaccca
     2221 tattgatatc aataatctgg gcaccgcttt ccacgttaat acgtgctgca tctgccattt
     2281 ctttcggagc gctaccagca atttgcacgg tgcgaatacc gggttcatca atgtgcacca
     2341 tccgtaaacg agatttgtcg ctttcccaaa cctgtgggtt agaagacatc atctcggata
     2401 ctgtcaatcc ggctcccatc tcgtagcaca acgtccgaaa aggtctgtct gtaatgccag
     2461 ccatgggcgc tgcgatcagg cgatttctga gctgatattg tccgatgcgc atgagttaag
     2521 aaatgaccat actgtgactg caaggcggcg tatattacgc attttttgca cgagatgaaa
     2581 ggccaaactt tgaccaatcc tctgagatgg atcaaagaat tgcatttaaa atgagcgtgg
     2641 tgcgataatt actcataaaa atcatcatat tagaaaatag tgactaaaat ttacactcaa
     2701 agaaatttga gtaagttctc aatttttctt tatgaatgaa aattttggca cgcaaatttg
     2761 cgtaaataat cggcaaattt acgtgccttt tgtgagcttg ctcgcacttc gccccgcgtc
     2821 accctacggc gatgcgaagg ttaattctta cgaccggtaa tacggcacca ctcttctttt
     2881 tccacgaccg ggtccagtgc gaagctatcg gcataagctt cacaaacgct ctctgcctgg
     2941 cttgccagaa taccggaaag gcccagcaaa ccgcctgaaa ccggcaggac gctgattaac
     3001 ggtgccagtt cacgtaatgg gcctgcaagg atgttagcga ccaccacgtc ggctttcatt
     3061 tcttctggct gatctttcgg taagtagagt tccagacggt cagaaacgcc attacgttcg
     3121 gcgttatcgc ggctggcctg aatcgcctgc ggatcgatat caataccaat ggcttttgct
     3181 gcacccagtt tcagcgccgc gatcgccaga atgccggaac cacagccaaa gtcgatgact
     3241 gttttaccgg ttaaatcgag gctgtcgagc cattgcaggc acagagaggt ggttggatgg
     3301 gtacccgtac caaacgccag ccctggatct aacatcacgt tgacggcgtt ttcgtccggc
     3361 acatcacgcc agctagggca gatccacagt cgttcaccaa agcgcatcgg gtggaaatta
     3421 tccatccatt cgcgctccca gtctttatct tctagttgtt cggttttatg cgcgaagcct
     3481 gcgccgagca gcggatggtt ttccagaatc gccaccacgt cgttcatatc ggtttcagcg
     3541 tcgaacagac caatcacatc ggtgtcgccc cacaggcgcg tttcgcccgg cagcggctca
     3601 aatactggcg tatcgtgggt atcctgaaaa gtgatagaaa cggcacccgc ttccatcagc
     3661 gcatcgctaa gatcttccgc gttcgcgccg gtggtgttca gtttcagttg gatccaaggc
     3721 atggcaaaac tctttattta tcagtagtca aaacggtagc ttgcgggacg gatgtaccga
     3781 aacggtttcc gaccaggaaa gccagcaaac ttagtagtaa cgagggcacg atagggtgga
     3841 agcccaggta ctgaatattc agcgtcgcga gtacggcata cagcacgccg ccaacgatca
     3901 tcgcacttag cgcgcctttg gcgttggcgc gttcccagta aagacccagc accagcggcc
     3961 acaggaaaac ggcttccagc ccaccgaagg ccagcaaatt cagccagatg atcatttctg
     4021 gcggcttcca ggcggcaagc agcagcaacg cgccgagaac taacgtaatt accgccgaca
     4081 tccgcttcag acgcgtctcg ttttgcattt gatccggacg gatattcaga tagagatctt
     4141 taatgatcgt agcggaactt tgcagcagtt gggcgttaat tgtcgacatg atcgcagcca
     4201 taggtgcagc cagaaagatc ccggcagcaa aaggtggcag cacttttacc attaacgtcg
     4261 ggatcaccag atccggtacg gtgagatcgg ggatcactgc acgacctaac gctccggcta
     4321 ggtgcatacc gaacatcagg attgcgacta caatcgtacc gatgatgatc ccccggtgta
     4381 ctgctttgct gtctttataa gagatacagc gcaccgcagt atgcggcagg ccaatcacgc
     4441 caaagcacac cagtacccag aacgacgtca taaaggcagg cgacagaata tcgtcagcgc
     4501 cttgtggcgt aaccagttgc ggatcgatgg tttgcaaggt ctgtactgcg ttgcttaagc
     4561 cgccagcggc atgtaccacg ccaataagca gcacaacggt gccaatcagc atcacaagcc
     4621 cttgcatggt gtcgttcagc acgctggcgc gaaagccacc aaaggcggta tataacgcaa
     4681 tgctgatacc aaaaatcagc agcccggttt cataaggaat acccgccgcg gtttccagca
     4741 ggcgcgcacc gccgataaac tgcacggtca tcgcaccaac gaacgcaacc agcaaactca
     4801 aactcgccag ccacaccaga agacgactct ggtagcgggc aaacagcata tcgttcagcg
     4861 tcactgcatt gtagcggcgc gcaagaatcg caaacttctt gccgagaata ccgagtgaaa
     4921 gccagacagc aggaagctga atcatcgcca gcaataccca gcccagcccg tatttataag
     4981 cagctcctgg cccgccgata aacgaactgg cactgatata ggtcgcggtg agcgtcatcg
     5041 ccagcacaat accgcccata gagcggctgc cgaggaaata ctcattaagg aaggtgccgg
     5101 tgctccgttt acgcatcgca taaaccgaga taccgaacac caccaccaga taggcgacca
     5161 gcggtagaat tacttcaagc tgcatcgtca tcctccagtg ggatatcgcg atagataaat
     5221 ttcaccatcg cccagcacag tccaataaac agtagcggcg tcaggatgca ggccatctca
     5281 aaccagcgcg gaaagccggt aaaaccgggg gcaacgccag gtaagtaagc atctactaac
     5341 caaactgcca gatacaaaag ggtcagcccc agcgcccagc gcgcctcttt atgggcctga
     5401 acaaaactag tgtccatttt ttgtccctga tgggtgaaga aagcgaggat tgtaccttat
     5461 gggggttgtc gatccccagt aataaaaaag gccggaaaat ccggcctttt gacgcttttg
     5521 cagtcttatt tttcctgaag accgagtttt ttctccagat agtggatgtt agtgccacca
     5581 tgctggaagt tctcgtcatt catgatgcgg atctgcagat caacgttggt tttgataccg
     5641 tcgatgatca gctcctgcag cgcattcttc atgcgggcaa tcgccacgtc acggttttca
     5701 ccgtagcaaa tcagcttacc gatcattgag tcatagtacg gcggtacggt gtagcccgcg
     5761 tagatatgag actcccaacg tacgccaaaa ccgccaggtg cgtggaaacg ggtgattttg
     5821 cctgggcttg gcaggaaggt gttcggatct tcggcgttga tacgacattc caccgcatgg
     5881 ccgcgaacgt gaacttcttc ttgcttgatc gacagcggtt gaccggcagc gatacgcagc
     5941 tgttctttga tcaggtcaac gccggtgatc atttctgtaa ccgggtgttc tacctgaata
     6001 cgggtgttca tttcgatgaa atagaactcg ccgttttcga acaggaactc gaaagtacct
     6061 gcaccgcgat agccgatatc aacacacgct ttagcgcaac gttcgccgat gtagcgacgc
     6121 agttccgggg taatgcccgg tgctggtgct tcttcgacca ctttctggtg gcggcgctgc
     6181 atggagcagt cacgttccgc cagatagata gcgttgccct gaccgtcagc cagtacctga
     6241 atctcgatgt ggcgaggatt ctccaggtat ttctccatgt aaaccatatc gttgctgaaa
     6301 gcagctttcg cttccgcacg ggtcatggag atggattgtg ccagttcagc gtcgccgcgc
     6361 actacgcgca taccgcgacc gccgccgccg ccggaggctt tgataatcac cggataacca
     6421 atgcgtttag caatggcgcg gtttttatcc atatcgtcgc ccagcgggcc gtcagaacca
     6481 ggtacgcaag gaacgcccgc ttttttcatc gccgcgattg cggatacttt gtcgcccatc
     6541 aggcgaatgg tttctgcttt cgggccgatg aagataaagc cggagcgttc aacctgctcg
     6601 gcaaagttgg cgttctcgga gaggaagccg taacccggat ggattgctac tgcgccggtg
     6661 atttcagcgg cgctgatgat tgccgggatg ttcagataac tttgtactga cggagcaggg
     6721 ccaatacaga ccgtttcatc tgccagtaat acgtgtttta gatcgcgatc cgcgctggag
     6781 tgcacagcga cagtcttgat gcccagttct ttacaggcac gaagaatacg caatgcaatc
     6841 tcgccgcggt tggcaataac aattttatcc agcatgttcg cctcgttact cgatgacgac
     6901 cagcggctcg tcaaattcta ccggttgtcc actttcgacc agaattgctt tcacggtacc
     6961 ggatttgtcc gcttcgatct ggttcatcat tttcatggct tcaacgatgc acagggtatc
     7021 gcccacgttg actttctgac ccacttcgat gaacgctttt gcgtccgggc ttggggtgcg
     7081 gtagaaagta ccaaccatcg gggaacgtac gatgtgacca ctgatttccg ctgctgctgg
     7141 cgcttccatg gaaggaacgg tcgccggagc ggctgcgtta gattgagctg gctgctgcat
     7201 cattggtgca gcgtaagctt gttgcatcac agggaaactt gcggcaggag ctgcacggct
     7261 aatgcgtact gactcttcgc cttcagaaat ttccagttcg gagatgcctg attcttcaac
     7321 cagctcgatc agttttttaa tcttacgaat atccatgagt gggttccgta ctctttgttt
     7381 agtgtgattg tgacaggcgt ttcaccgccg tctgtaaagc gtatgccccg acagctgtat
     7441 gcatagcgat aaattccagc aggccgggac gccggtctat tttgccccag agtgtcaatg
     7501 ttagacttga cggacattgt gcagcgatct gctgctatcc tccggcaaaa aacaaaatat
     7561 accttcgttg cgccaatgtc accttttccg cagcgcaaaa actgcgtcag ggaggacgag
     7621 gtcgcacatt ataacgattt cgtagcaatt ggcagctaaa tactggtctt atcagggaag
     7681 ataatcaaca gctaacatgt aaataacctt caacaccgtg taatttgcaa caagccgcac
     7741 aattcacgaa attagcgcca tcatcgacgg aacttcttat aacgtaaggt gaaaagctga
     7801 aaagccagcc ctgcgaagat gaggggctgc aatgagataa tcttcacaga ccacaaataa
     7861 tgtagggatg ccaggatcgc gacaagatag acgacattac gcagcggttg ctggggatta
     7921 cccaattttc actgcatcgt ctggatagat gtgaatgcta aagcacgcaa aatcaaccaa
     7981 ctgacaattc tcagcattaa ataggttcga gtggttaatt tgtagtcaaa taaaatcaga
     8041 ttattcaccc catggtacca gaaatcagta acaggttaag cgtagtgtcg cccaggcaaa
     8101 gcaccataaa cctaacaggc gacggaggcg tatcaataat ggctgtttag aggagtatca
     8161 atgcgcatca atttgtggtc agtcgaaatc atgactttcc agtttggatg atgttgtaca
     8221 acttcctgac caaggtgttc gacgaaagct gtccggctaa ccgcttttcc cgttaaccga
     8281 ttgaataatg atgtcaaaat gagttccctc tcttattatt ccttgctaaa tggttagtta
     8341 accttcacca gcgtgcgacc ttggatctgg ttattaatga tggcctcggc aaagttcggt
     8401 gcctctgaca gagatatctc ttttgccgcc tgggtataga atgattccgg taaatcggcg
     8461 accagtcgct gccaggcttg tgcgcggcgt tctggtggcg tcattactga atccacccct
     8521 tgcaaacgga cattacgcag aataaatggc atgaccgtgc ttggcagagt aaaaccaccc
     8581 gccagaccac aggccgccac gcagccgccg taattcattt gcgccagcac ttttgccagg
     8641 actttgtcgc caacagtgtc aattgcccca gcccagactt gtttttccag aggacgggat
     8701 tcggcaaact catcacgagg gagaatacgg ctggcaccta aacttttcag atattcatgg
     8761 gtactttcgc gaccggaaac ggcaacgacc tgataaccca acttatgcag cagcgccacg
     8821 gcggtactgc cgacgccacc actggcaccc gtcacgacaa tctccccgtc ctgcgggcga
     8881 acaccggcat cttccagcgc catcacacac agcatggcgg taaaaccggc agtaccgata
     8941 atcattgctt tacgcgcgtc cagcccttgc ggcatggcaa ccagccagtc acctttcact
     9001 cgcgcctgct ccgccagccc accccagtgg ttttcaccaa cgccccagcc agtgagtaac
     9061 acctcctgac cggcatgaaa acgcggatct tcgctggtgc gtacagttcc ggcaaaatcg
     9121 atcccaggaa tcatcggaaa attacggatg atttttccct taccggtaat cgccagcgca
     9181 tctttatagt tcaggctcga ccagtgaaca tcgaccgtga catcgccctc cggcaggcga
     9241 ctttcgtcca gagtctgtac tgatgcgaga gttttgccgt cctgctgttc taaaagtaac
     9301 gcctgcataa gtggtcctca tgtgcatgat ggattggaaa ataatttctg aagactatac
     9361 tcgctaatgg aaaagcaaag ccgatgaagc gcaataaatt gcgagataaa tctgatttgc
     9421 tagtatgccc gcttcctcac tatcggagtt aacacaagga tgagattaac gacgaaattt
     9481 tcggcctttg ttacgctgct caccgggtta acaatttttg tgactttgct gggctgttcg
     9541 ctaagtttct acaacgccat tcagtataag tttagtcatc gcgttcaggc ggtggcgacg
     9601 gcgattgata cccaccttgt gtcgaatgac ttcagcgcat taaggccaca aattaccgaa
     9661 ttaatggtgt cagcagatat cgttcgtgta gacctgctgc atggtgataa acaggtttat
     9721 accctggcca gaaatggtag ttatcgtccg gttggcacca gcgatctgtt tcgtgaactg
     9781 agcgttccgt tgataaagca tccggggatg tcgttgcgtc tggtttatca ggatccgatg
     9841 ggcaactatt tccattcgtt gatgaccacc gcgccgctca cgggggcgat tggctttatc
     9901 attcttatgc tctttctggc ggtacgctgg ttacaacggc aacttgccgg gcaagaattg
     9961 ctggaaaccc gggctactcg tatcttaaac ggtgagcgtg gctctaatgt gttgggaacc
    10021 atctatgaat ggccgcccag aaccagcagt gcgctggata cgctgcttcg tgaaattcag
    10081 aacgcacgcg aacaacacag ccgtcttgat acgctgatcc gctcttatgc cgcacaggac
    10141 gtgaaaaccg gcctcaataa ccgactcttt ttcgataatc agttagcaac gttactggaa
    10201 gatcaggaga aagtaggtac ccacgggatc gtgatgatga ttcgtctgcc ggatttcaat
    10261 atgttgagcg atacctgggg gcacagccag gttgaagaac agttcttcac tctgacgaat
    10321 ctgctgtcga catttatgat gcgctaccct ggcgcactgc tggcgcgtta ccaccgcagt
    10381 gattttgctg cgctgttacc gcaccggacg ttaaaagagg cagagagcat agccagtcag
    10441 ttaatcaaag ccgtcgatac cttgccgaac aataaaatgc tcgatcgcga cgatatgatc
    10501 cacattggta tctgtgcctg gcgtagtggt caggataccg agcaggtaat ggaacatgca
    10561 gagtctgcca cgcgtaatgc gggattgcag ggtggcaata gctgggctat ttatgatgac
    10621 tcgttgcctg aaaaaggacg cggtaatgtt cgctggcgta cgcttatcga gcaaatgctc
    10681 agtcgcggcg gcccgcgcct ttatcaaaaa ccggcggtta ctcgcgaagg tcaggttcat
    10741 catcgcgaac tcatgtgccg catcttcgat ggtaatgaag aggttagctc ggcggagtat
    10801 atgccgatgg tcttgcagtt tggcttatcg gaagagtatg accgtctgca aatcagccgt
    10861 cttattccac tattgcgtta ctggccagag gaaaatctgg cgattcaggt taccgttgag
    10921 tcgctgattc gtccgcgttt tcagcgttgg ctgcgcgata cgttaatgca atgtgaaaaa
    10981 tcacaacgaa aacgcataat tattgaactt gcagaggccg atgtaggtca acatatcagt
    11041 cgtttacaac ctgttattcg tttagtgaat gctttagggg tacgggtagc cgtcaaccag
    11101 gctggtttga cgctggtaag caccagttgg atcaaagaac ttaatgttga gttactcaag
    11161 ctccatccgg ggctggtcag gaacattgag aagcgaacgg agaaccagct gctggttcaa
    11221 agcctggtgg aagcctgctc cgggaccagc acccacgttt acgccaccgg cgtgcgttcg
    11281 cgaagcgagt ggcagaccct gattcagcgc ggtgttacag gcgggcaagg ggattttttc
    11341 gcgtcctcac agccacttga tactaacgtg aaaaaatatt cacaaagata ctcggtttaa
    11401 cctgccgttt aatccgtttt cacgtagaat aacgcgcgct gcgtctcatg ggagtgtgct
    11461 tgtctgctcg ccagattgtt gcagcacata tgcagatgaa tgaccttacg cggttgcaaa
    11521 caggcgagga atgctgctga tgcattaagc ctttctggac tcaggcagag atttgtaaca
    11581 aaggaaacga actgcactaa ttttcaccgt agcagatgat ttttgcgcct tgtcgctgct
    11641 gcgtgtggtt ggtaaagtaa gcggattttc ttttccgccc cagctttcag gattatccct
    11701 tagtatgttg aaaaaatttc gtggcatgtt ttccaatgac ttgtccattg acctgggtac
    11761 tgcgaatacc ctcatttatg taaaaggaca aggcatcgta ttgaatgagc cttccgtggt
    11821 ggccattcgt caggatcgtg ccggttcacc gaaaagcgta gctgcagtag gtcatgacgc
    11881 gaagcagatg ctgggccgta cgccgggcaa tattgctgcc attcgcccaa tgaaagacgg
    11941 cgttatcgcc gacttcttcg tgactgaaaa aatgctccag cacttcatca aacaagtgca
    12001 cagcaacagc tttatgcgtc caagcccgcg cgttctggtt tgtgtaccgg ttggcgcgac
    12061 ccaggttgaa cgccgcgcaa ttcgtgaatc cgcgcagggc gctggtgccc gtgaagtctt
    12121 cctgattgaa gaaccgatgg ctgccgcaat tggtgctggc ctgccggttt ctgaagcgac
    12181 cggttctatg gtggttgata tcggtggtgg taccactgaa gttgctgtta tctccttgaa
    12241 cggcgtggtt tactcctctt ccgtgcgcat tggtggtgat cgtttcgacg aagctatcat
    12301 caactatgtg cgtcgtaatt acggttctct gatcggtgaa gccaccgcag aacgtatcaa
    12361 gcacgaaatc ggttcggctt acccgggcga tgaagtccgt gaaatcgaag ttcgtggccg
    12421 taacctggca gaaggtgttc cacgcggttt taccctgaac tccaacgaaa tcctcgaagc
    12481 actgcaggaa ccgctgaccg gtattgtgag cgcggtaatg gttgcactgg aacagtgccc
    12541 gccggaactg gcttccgaca tctccgagcg cggcatggtg ctcaccggtg gtggcgcact
    12601 gctgcgtaac cttgaccgtt tgttaatgga agaaaccggc attccagtcg ttgttgctga
    12661 agacccgctg acctgtgtgg cgcgcggtgg cggcaaagcg ctggaaatga tcgacatgca
    12721 cggcggcgac ctgttcagcg aagagtaatc ggatgcaggc aggggaagcg tctgtttacc
    12781 ctgcctggtc tgatacgaga atacgcataa cttatgaagc caatttttag ccgtggcccg
    12841 tcgctacaga ttcgccttat tctggcggtg ctggtggcgc tcggcattat tattgccgac
    12901 agccgcctgg ggacgttcag tcaaatccgt acttatatgg ataccgccgt cagtcctttc
    12961 tactttgttt ccaatgctcc tcgtgaattg ctggatggca tatcgcagac gctggcctcg
    13021 cgtgaccaac tagaacttga aaaccgggcg ttacgtcagg aactgttgct gaaaaacagt
    13081 gaactgctga tgcttggaca atacaaacag gagaacgcgc gtctgcgcga gctgctgggt
    13141 tccccgctgc gtcaggatga gcagaaaatg gtgactcagg ttatctccac ggttaacgat
    13201 ccttatagcg atcaagttgt tatcgataaa ggtagcgtta atggcgttta tgaaggccag
    13261 ccggtcatca gcgacaaagg tgttgttggt caggtggtgg ccgtcgctaa actgaccagt
    13321 cgcgtgctgc tgatttgtga tgcgacccac gcgctgccaa tccaggtgct gcgcaacgat
    13381 atccgcgtaa ttgcagccgg taacggttgt acggatgatt tgcagcttga gcatctgcca
    13441 gcgaatacgg atattcgtgt tggtgatgtg ctggtgacct ccggtctggg cggtcgtttc
    13501 ccggaaggct atccggtcgc ggttgtctct tccgtaaaac tcgataccca gcgcgcttat
    13561 actgtgatcc aggcgcgtcc gactgcagga ctgcaacgtt tgcgttatct gctgctgctg
    13621 tggggcgcgg atcgtaacgg cgctaacccg atgacgccgg aagaggtgca tcgtgttgct
    13681 aatgaacgtc tgatgcagat gatgccgcag gtattgcctt cgccagacgc gatggggcca
    13741 aagttacctg aaccggcaac ggggatcgct cagccgactc cgcagcaacc ggcgacagga
    13801 aatgcagcta ctgcgcctgt tgcgccgaca cagcccgctg ctaatcgctc tccacaaagg
    13861 gctacgccgc cgcaaagtgg tgctcaaccg cctgcgcgtg cgccgggagg gcaatagtgg
    13921 cgagctatcg tagccaggga cgctgggtaa tctggctctc tttcctcatt gcgctgttgc
    13981 tgcaaatcat gccctggccg gataacctga ttgttttccg gccaaactgg gtgttactta
    14041 tcttgttgta ttggatcctg gccttgcccc atcgcgtaaa tgtgggcaca ggttttgtga
    14101 tgggtgccat actggatctg atcagcggct cgatgcttgg cgtacgcgta ttggcgatga
    14161 gcatcattgc ttacctggtg gcgctgaaat accagctttt ccgcaacctc gcattatggc
    14221 agcaggcgct ggtcgtcatg ttgctttcgc tggtggtgga tattattgtt ttctgggcag
    14281 agtttttagt gattaacgtc tctttcagac cggaagtgtt ctggagtagt gtagtcaatg
    14341 gggtgctctg gccgtggatt ttcttgctga tgcgtaaagt ccgtcagcag tttgccgtgc
    14401 aataaaggtt tctatgactt ctctgtattt agcttccggt tctccgcgtc gtcaggagtt
    14461 acttgcgcaa cttggcgtga cctttgaacg tattgttacg ggcattgagg agcagcgtca
    14521 gccgcaggag agcgcgcagc agtatgttgt gcgtctggca cgcgagaaag cacaggcggg
    14581 tgtcgcgcaa acggcacagg atctcccggt gctgggtgcg gatattatcg ttatcctgaa
    14641 cggagaagtg ctcgagaaac cgcgcgacgc agagcatgcg gcgcagatgt tgcgcaaatt
    14701 atcgggtcag acccatcagg tgatgacggc agtggcgttg gccgacagcc agcatattct
    14761 cgattgcctg gtggtcaccg atgtgacttt cagaacgtta acagacgaag acatcgcggg
    14821 ctatgtcgcc agcggtgaac cgttagataa agcaggtgca tacggtattc aggggctggg
    14881 tggctgtttt gtcaggaaga taaatggcag ctatcacgcc gtagtcggct taccgctggt
    14941 tgaaacgtat gaattattaa gtaattttaa cgcactgcgt gagaaaaggg ataaacatga
    15001 cggctgaatt gttagtaaac gtaacgcctt cggaaacgcg agtggcgtat attgatggcg
    15061 gtattctgca ggaaattcat attgaacgtg aggcgcgacg cggaatagta ggcaatatct
    15121 acaagggtcg tgtaagtcgt gtacttccgg gtatgcaggc ggcttttgta gatattgggc
    15181 tggataaagc cgcgtttctt catgcatccg acatcatgcc gcataccgaa tgtgtggcgg
    15241 gtgaagaaca aaagcaattc acggtgcgcg acatctcgga actggttcgt caggggcaag
    15301 atctgatggt gcaggtggtg aaagatccgc ttggcactaa aggtgcgcgc ctgaccaccg
    15361 atatcacgct gccttctcgc tatctggtat ttatgccagg ggcttctcac gttggggttt
    15421 cccaacgaat tgaaagcgaa tcagaacgtg aacgcctgaa aaaagtggtc gcagagtatt
    15481 gcgacgagca gggcgggttt atcatccgca ctgcagcgga aggggttggc gaggctgaac
    15541 tggcctccga tgccgcttat ctgaaacgcg tctggaccaa agttatggag cgtaaaaagc
    15601 gcccgcagac ccgttatcag ctgtacggcg aactggcgct ggcgcagcgt gttctgcgtg
    15661 attttgccga cgccgaactg gaccgcattc gcgttgactc acgcctgact tacgaagcat
    15721 tgctggagtt tacctcggag tacattcccg agatgaccag caagctggag cattacaccg
    15781 gacgccagcc gattttcgat ctctttgatg tcgaaaacga aatccagcga gcgttggaac
    15841 gcaaagtaga actgaaatcc ggtggttatc tgattatcga ccagaccgaa gcgatgacca
    15901 ccgtggacat caataccgga gcgtttgtcg gtcatcgcaa tctggacgac actattttca
    15961 ataccaatat tgaagcgacg caggctatcg ctcgccagtt acggttgcgt aatctgggcg
    16021 ggattatcat tattgatttc atcgatatga ataatgaaga tcaccgccgc cgcgtgctgc
    16081 attcgctgga gcaggcgttg agcaaagacc gggtgaaaac cagcgttaat ggtttttcgg
    16141 cgctggggct ggtggagatg acgcgtaaac gcacccgcga aagcattgag cacgtactgt
    16201 gtaacgaatg cccaacctgc cacggtcgcg gaacggtgaa aaccgtggaa acggtatgct
    16261 atgaaatcat gcgcgagatt gttcgtgtcc accatgctta cgactccgac cgtttcctgg
    16321 tctatgcttc tccggcagta gccgaagcct tgaaaggcga agagtcacac tcgctggcgg
    16381 aagtggaaat tttcgttggc aaacaggtta aagtacaaat tgaaccgctc tataaccagg
    16441 agcagtttga cgtcgtaatg atgtaaacag atgctgggcc gccatccggc aaagggtttt
    16501 tgagtcacat ttttagcaga caaggagtga cgggtgaggc gattgccggg gattttactg
    16561 cttactggag ccgcgctcgt tgtgatcgct gccctgctgg ttagcggcct gcgtattgct
    16621 ttaccgcatc ttgacgcctg gcgtccggaa atcctcaaca aaatagaatc cgcgactggc
    16681 atgccggtag aagccagtca gctctcagcc agctggcaga attttggccc gacgcttgaa
    16741 gcacacgata tccgtgcaga actaaaagat ggcggcgaat tttcggttaa acgcgttact
    16801 ctggcgctgg atgtctggca gagcctgtta catatgcgct ggcagtttcg cgacctcact
    16861 ttctggcagc tgcgctttcg caccaacact cctatcacca gcggtggtag cgatgacagt
    16921 ctggaagcca gtcacatcag cgatctgttt cttcgtcaat ttgaccattt cgatctccgc
    16981 gacagtgaag tcagttttct gacgccatcc ggtcagagcg ccgagctggc gatcccacaa
    17041 ctcacctggc tgaacgatcc acgtcgacac cgtgcggaag gcctggtaag cctctccagc
    17101 cttaccgggc agcacggcgt gatgcaggtg cgcatggatt tgcgcgatga tgaggggttg
    17161 ttaagcaatg gtcgcgtctg gctacaggcg gatgacatcg acctgaagcc gtggctcggt
    17221 aaatggatgc aggacaatat tgctctggaa acggcacagt tctcccttga aggctggatg
    17281 acgatcgaca aaggcgatgt aaccggcggt gacgtctggc tgaaacaggg cggtgccagc
    17341 tggttgggcg agaagcaaac gcatacgctg tcggtggata atctgaccgc gcatattacg
    17401 cgtgaaaatc cgggctggca gttctctatt cccgatacac ggatcacgat ggacggcaaa
    17461 ccctggccga gcggagcatt gacgctggcc tggataccgg aacaggacgt tgacggcaaa
    17521 gacaataaac gcagtgacga actccggatt cgcgctagta atctggagct ggcaggcctg
    17581 gagggcatac gcccgctggc cgcgaaactt tcacctgcac tgggtgatgt ttggcgctcc
    17641 acacaaccga gcggcaagat taacactctg gcgctggata tcccgcttca ggcggcagac
    17701 aagacccgtt ttcaggcatc gtggagcgat ctggcctgga agcaatggaa attattaccg
    17761 ggtgcggaac acttctccgg gacgctttcc ggcagcgttg aaaatggttt gcttaccgcg
    17821 tcgatgaagc aggcaaagat gccttacgaa acggtattcc gtgcgccact ggaaatcgcc
    17881 gacggccagg caactataag ctggctgaac aatgacaaag gtttccagct ggatgggcgt
    17941 aatattgacg ttaaagccaa agccgtccat gcgcgcggcg gttttcgtta cctgcaacct
    18001 gctaacgatg aaccctggct gggtattctg gctggcatca gtaccgatga tggttcacaa
    18061 gcctggcgct atttcccgga aaacttgatg ggtaaagacc tggttgatta tttaagtggt
    18121 gcgattcagg gcggtgaagc ggataacgcg acgctggttt atggtggcaa tccgcaactc
    18181 ttcccctata aacacaacga aggtcagttt gaagtgctgg tgccgctgcg caacgcgaag
    18241 tttgccttcc agccggactg gcctgcatta actaaccttg atattgaact ggactttatt
    18301 aacgacggtt tatggatgaa aaccgatggc gttaatctgg gcggcgtgcg cgcgagtaat
    18361 ctcaccgcag tgatccctga ctactcaaaa gaaaaactgc tgattgacgc tgacattaaa
    18421 ggtccgggta aagccgttgg cccttacttt gatgagacac cgctgaaaga ttctctgggt
    18481 gcgaccctgc aagaactcca gctcgacggc gatgtgaatg ctcgcttaca tcttgatatc
    18541 ccgctgaacg gcgaactggt aaccgcgaaa ggtgaagtga cgctgcgtaa taacagtctg
    18601 tttatcaaac cactcgacag catcctgaaa aatttgagcg gtaaattcag ctttatcaat
    18661 ggcgatctgc aaagtgaacc actgacagca agctggttta atcagccgtt gaacgtggat
    18721 ttttccacca aagaaggggc aaaagcctac caggttgcgg tgaatctcaa cggtaactgg
    18781 caaccggcga aaaccggcgt tctgcctgaa gcggtgaacg aagcattgag tggcagcgtg
    18841 gcgtgggatg gtaaagtggg cattgatctg ccttatcatg ctggcgcgac ctataacgta
    18901 gagctgaacg gcgatctgaa taatgtgagt agtcacttac cttcaccgtt agccaaacct
    18961 gcgggtgaac cactagcggt aaacgttaag gttgatggca atctcaacag ctttgaatta
    19021 accggacagg ctggtgcgga taaccatttc aatagccgct ggttgcttgg tcaaaagctg
    19081 acgctcgatc gtgctatttg ggcggcagac agtaaaacgc tcccgccgtt gccggaacaa
    19141 agtggcgttg aacttaatat gccgccgatg aatggtgccg agtggctggc cctgttccag
    19201 aaaggcgctg cggagagtgt cggtggtgca gcgagtttcc cacaacacat aacattacgt
    19261 acgcctatgt tgtcactggg aaatcagcaa tggaataacc tgagtattgt ttcgcaaccg
    19321 acggcaaatg gcaccctggt tgaggcgcaa gggcgtgaaa tcaacgccac gctagcgatg
    19381 cgtaataacg cgccgtggct ggcgaatatc aaatatcttt attacaaccc gagcgtggcg
    19441 aaagctcgtg gtgattcaac gccgtcatca cctttcccga caacggagcg cattaacttc
    19501 cgtggctggc cggacgcaca aatacgatgc acagagtgct ggttctgggg gcaaaaattc
    19561 ggtcgcattg acagtgatat caccatttct ggcgatacgt taacgctgac caatggactg
    19621 attgatactg gtttctcgcg gcttactgcc gatggtgaat gggttaataa tccggggaat
    19681 gaacgtacct cgctgaaagg aaaactgcgt gggcagaaaa ttgatgccgc cgcagaattt
    19741 tttggtgtca cgacgcccat acgccagtcg tcatttaatg tggattacga tttacactgg
    19801 cgcaaagcac cctggcagcc agatgaagcg acgttgaatg gcatcattca tactcaactg
    19861 ggtaaaggcg aaattaccga aatcaatacc ggacatgccg ggcaattgct gcgcttattg
    19921 agcgtagatg ccctgatgcg taagctgcgt tttgatttca gagacacttt tggcgaaggg
    19981 ttctattttg actccattcg cagcaccgcg tggattaaag acggcgttat gcacaccgac
    20041 gacacgctgg tggatggcct ggaggcggat atcgccatga aagggtcggt aaatctggta
    20101 cgtcgagacc tgaatatgga agcggttgtc gcaccagaga tttctgcgac ggtgggcgtg
    20161 gctgcggctt ttgcggttaa ccccattgtt ggcgcggcag tgtttgccgc cagtaaagtg
    20221 ctggggccgc tgtggagcaa agtctccatt ttgcgctatc acatttcggg tccgctggat
    20281 gatccgcaaa tcaacgaagt gttgcgccaa ccgcgtaaag aaaaagcgca atgatttgac
    20341 gagggcgcgt aattgcccca atctcatatg ataatcgttg ccaaaggcca acgagccgga
    20401 acataactgt aggccggata gggcgttcac gccgcatccg gcagccgtaa aaaatcctct
    20461 actgcagtaa ctaacgagta gcaaaaacga tgagtctgaa cctggtaagt gaacaattgc
    20521 tagcggcgaa cggcctgaaa catcaggact tgttcgcgat cctcggtcaa ctggccgaac
    20581 gtcgccttga ttatggcgat ctctattttc agtcgagcta tcacgaatcc tgggttttag
    20641 aagaccgcat tattaaagat ggttcttaca acatcgatca gggcgttggc gtgcgtgcaa
    20701 tcagcggtga aaaaaccgga tttgcttacg ctgaccaaat cagcctgctg gcgctggaac
    20761 agagtgcgca agcggcgcgc accatcgtcc gtgatagtgg tgatggcaaa gtacagacgc
    20821 tgggcgcggt agagcatagc ccgttgtata cctcggtaga tccgctgcaa agcatgagcc
    20881 gtgaagagaa gctggatatc ctgcgtcgcg tcgataaggt tgctcgcgaa gcggacaagc
    20941 gcgtacagga agtgactgcc agcctcagtg gtgtctatga attaattctg gttgcggcca
    21001 ccgacggcac gctagcggcg gatgtccgtc cgctggtgcg tctttccgtg agcgttctgg
    21061 tcgaagaaga tggcaaacgc gaacgcggtg ccagtggcgg cggcggtcgt tttagttatg
    21121 agttcttcct tgccgatctt gacggcgaag tccgtgcgga tgcatgggca aaagaagcag
    21181 tgcgtatggc gctggtcaat ctttctgccg ttgctgcacc agcgggcacc atgccggtag
    21241 tacttggcgc aggttggccg ggcgtgctgt tgcatgaagc ggtaggtcac ggtctggaag
    21301 gcgacttcaa ccgccgtggc acttcagtat ttagtggaca ggtcggggag ctggtggctt
    21361 cagaactgtg taccgtggtt gatgacggca cgatggtcga tcgccgtggt tcggtggcga
    21421 ttgatgacga aggtacgcca ggccagtaca acgtgctgat tgagaacggc attctgaaag
    21481 gctacatgca ggataaactc aacgcgcgtt tgatggggat gacgccgact ggcaacggtc
    21541 gccgtgaatc ctacgcccat ctgcccatgc cgcgtatgac caacacctat atgctgccgg
    21601 gtaaatcgac cccgcaagaa attattgaat ccgttgagta cggtatctat gcaccgaact
    21661 ttggtggcgg tcaggtggat atcacctccg gcaaattcgt tttctccact tcagaagcat
    21721 atctgattga aaacggtaaa gtaacgaagc cggtgaaagg cgcaacgttg attggttccg
    21781 gtatcgaaac catgcagcag atttcgatgg ttggcaacga cctgaaactg gataacggcg
    21841 tgggtgtctg cggtaaagaa gggcaaagtt tgccggttgg cgtgggccag ccaacgttga
    21901 aagtcgataa cctgactgtt ggcggtactg cgtaataatt tcattatttt cagaaggata
    21961 attaatcttt ctacgtgcac gaacggtccc ctcgctcctc tggggttagg gtgaggggaa
    22021 cccgttggca caggtttgta gaacgtaaca gtacaatatg aattacttct ctttcccgcg
    22081 cccgtgcatc tcctgaaaca atttaccgac ctcaacaaaa taatccgtca gcgagttgat
    22141 cacgacctgt accttcagcg gcagcttatc tttttcggta tataacgcat aaaccgggcg
    22201 tggatctgac tgataacgcg gcagcaggat ctccagctcc ccacgattga tctcgttgat
    22261 cacccacatc agcggcacgt aggcgatccc ggcacccgcc gttagccagc gcaccagcgt
    22321 catcggatca ttagtcacaa atctcccttg cgggatcagg cgagtcgaga tcccttccgg
    22381 tgcaatcagt tcaaattcat tgtcgggccg cacgctgtat tcaagccatg aatggctact
    22441 taaatcggcg ggtttttccg gtatgccgta ttgtgtgaga tagcttttcg cggcgcacac
    22501 caccattggc atcgcgccaa gacggcggga aaacaggctg gaatcctgca acgcgccgac
    22561 gcggatcacc acatccagac cgtcggcaat caggtcgggg gctggaattc cggtaaccag
    22621 attggcgctc aaacctgggt attctttcag cattttggct gtcagcccgg cgagaacatt
    22681 ttgtgccata gttgaagaac agccaatgcg tagcgtcccg atgggggtgt tattgaaggc
    22741 atacagttgc tcatgaacat cctgcacttc atgaagcata cgacggcagc cctggtagta
    22801 aattctaccg gcttcggtca ggccaatgct gcgtgtgcta cggtttaaca gctttacctg
    22861 caactcatct tccagttttg acaccgtctg actgatggac gaaacgctca tctgtagctg
    22921 tctggcggcg gcggtaaaag agccaaattc aactactttg gcaaacaccg acatgcgttt
    22981 tagtcgttcc attattcact ctgacttaaa agtgatttag atcacataat atagataaca
    23041 gcataacagt tacgctaata tattaaatat caatctacag caatgttgct ctcgcccggc
    23101 tttccatgcc cttctctgcg gcgacagatg ctgaaaataa taacgcctgc tctctctata
    23161 caaccaaggt caacatgagt ctgtttcccg ttatcgtggt gtttgggctg tccttcccac
    23221 cgatattttt tgaattgctt ttatcactgg cgattttctg gctggtgcgc cgggtacttg
    23281 tgccaacagg tatctacgac tttgtctggc atccggcgtt gttcaacacc gcgctctatt
    23341 gctgcttgtt ttatttgata tcgcgactgt tcgtttgagg ttgaagtgaa aacactaata
    23401 agaaaattct cccgtacggc catcacggtc gtattagtca ttctggcctt catcgcaatt
    23461 tttaatgcct gggtctatta caccgaatcc ccctggacgc gtgacgcgcg ctttagcgct
    23521 gacgtcgttg cgatcgcgcc ggacgtttct ggactcatta cccaggtgaa tgttcatgat
    23581 aaccagctgg tgaaaaaagg acagatactg ttcaccatcg accagccgcg ctatcaaaag
    23641 gcgcttgagg aagcgcaagc cgatgttgct tattatcagg tactggcaca ggagaaacgc
    23701 caggaggccg gacgtcgtaa ccgtctcggt gtgcaggcga tgtctcgcga agagatcgac
    23761 caggctaaca acgtactaca aacggttctg catcagttag cgaaagcgca ggcgacccgc
    23821 gatctggcaa aactggatct tgaacgcacg gtgatccgag cgccagcaga tggctgggtg
    23881 accaacctca acgtctatac cggtgagttt attactcgag gatcaacggc ggttgcgctg
    23941 gtgaaacaga actccttcta tgtactggcc tatatggaag aaactaagct ggaaggggtg
    24001 cgtccggggt atcgtgcaga gatcacgccg cttggcagta acaaagtgct gaaagggact
    24061 gttgatagtg ttgccgctgg ggtcaccaac gccagcagca cgcgtgacga caaagggatg
    24121 gcgaccatcg actctaacct tgaatgggtg cgtttggcgc aacgtgttcc ggttcgtatt
    24181 cgtctcgaca accagcaaga gaacatctgg cctgcgggca ccactgctac agtggtggtc
    24241 actggcaaac aagatcgcga cgaaagccag gattcgttct tccgtaaaat ggcccatcgc
    24301 ctgcgtgagt ttggttaatc acgatgggta ttttctccat tgctaaccaa catattcgct
    24361 ttgcggtaaa actggcgacc gccattgtac tggcgctgtt tgttggcttt cacttccagc
    24421 tggaaacgcc acgctgggcg gtactgacag cggcgattgt tgccgccggt ccggcctttg
    24481 ctgcgggagg tgaaccgtat tctggcgcta ttcgctatcg tggctttttg cgcatcatcg
    24541 gcacatttat tggctgtatt gccggactgg tgatcatcat tgcgatgatc cgcgcaccat
    24601 tattgatgat tctggtgtgc tgtatctggg ccggtttttg tacctggata tcctcgctgg
    24661 tacgaataga aaactcgtac gcgtgggggc tggccggtta taccgcgctg atcattgtga
    24721 tcaccattca gccggaacca ttgcttacgc cgcagtttgc cgtcgaacgt tgtagcgaga
    24781 tcgttatcgg tattgtgtgt gcgattatgg cggatttgct cttttctccg cgatcgatca
    24841 aacaagaagt agatcgagag ctggaaagtt tgctggtcgc gcaatatcaa ttaatgcaac
    24901 tctgtatcaa gcatggcgat ggtgaagttg tcgataaagc ctggggcgac ctggtgcgac
    24961 gcaccacggc actacaaggc atgcgcagca acctgaatat ggaatcttcc cgctgggcgc
    25021 gggccaatcg acgtttaaaa gcgatcaata cgctatcgct gacgctgatt acccaatcct
    25081 gcgaaactta tcttattcag aatacgcgcc cggaattgat cactgatact ttccgcgaat
    25141 tttttgacac gccggtagaa accgcgcagg acgtccacaa gcagctcaaa cgcctgcgga
    25201 gagttatcgc ctggaccggg gaacgggaaa cgcctgtcac catttatagc tgggtcgcgg
    25261 cggcaacgcg ttatcagctt ctcaagcgcg gcgttatcag taacacaaaa atcaacgcca
    25321 ccgaagaaga gatcctgcaa ggcgaaccgg aagtaaaagt agagtcagcc gaacgtcatc
    25381 atgcaatggt taacttctgg cgaaccacac tttcctgcat tctgggcacg cttttctggc
    25441 tgtggacggg ctggacttcc ggcagtggtg caatggtgat gattgcggtc gtgacgtcac
    25501 tggcaatgcg tttgccgaat ccacgcatgg tggcgatcga ctttatctat gggacgctgg
    25561 ccgcgctgcc gttagggctg ctctactttt tggtgattat ccctaatacc caacagagca
    25621 tgttgctgct gtgtattagc ctggcagtgc tgggattctt cctcggtata gaagtacaga
    25681 aacggcgact gggctcgatg ggggcactgg ccagcaccat aaatattatc gtgctggata
    25741 acccgatgac tttccatttc agtcagtttc tcgacagcgc attagggcaa atcgtcggct
    25801 gtgtgctcgc gttcaccgtt attttgctgg tgcgggataa atcgcgcgac aggactggac
    25861 gtgtactgct taatcagttt gtttctgccg ctgtttccgc gatgactacc aatgtggcac
    25921 gtcgtaaaga gaaccacctc ccggcacttt atcagcagct gtttttgctg atgaataagt
    25981 tcccagggga tttgccgaaa tttcgcctgg cgctgacgat gattatcgcg caccagcgcc
    26041 tgcgtgatgc gccgatcccg gttaacgagg atttatcggc gtttcaccga caaatgcgcc
    26101 gcacagcaga ccatgtgata tctgcccgta gcgatgataa acgtcgtcgg tactttggcc
    26161 agttgctgga agaactggaa atctaccagg aaaagctacg catctggcaa gcgccaccgc
    26221 aggtgacgga accggttcat cggctggcgg ggatgctcca taagtatcaa catgcgttga
    26281 ccgatagtta agtcaaaacc gacgccaaaa gcgtcggttt tttcatggct atacttagcg
    26341 atgcacggca gaactcgccg cgaaacgtga cggtggcaac agatgaatat ttatgccttt
    26401 gattttaatg agattgagag tcaggaggat ttttatcgtg actttagcca aacccttggt
    26461 ctggctaaag ataaggtacg cgatcttgac tcactatggg atgtgttaat gaacgatgtc
    26521 ctgccgctac cacttgagat tgaatttgtt catctgggag agaaaacgcg tcgccgtttt
    26581 ggcgcgttaa tattgctgtt tgatgaggca gaggaagagc tggaagggca tttgcgtttt
    26641 aatgttcgtc attagcgcaa aaaaaagccc ccgaaccggg ggcaatatcg tcggacaaaa
    26701 cgatgagggt ttatttgtac agttcagccg tagcgtgcca ggtgtcaccg ctacgagctt
    26761 cagtaatctg gtaggccgtt gcgcctttct cttccgcttt tttgttcagc atttcacgta
    26821 tatccattgg cgaagacgcc acaccactta cggatacggt cccgattgct tcacgatttt
    26881 gtgcttgtgc agcatcaatg gagtcggcag cgaatgcacc gaaagagaga acagaaagta
    26941 cgcttaatgc agctacagtg gttttgattt tcatgatttt tacctcgaca taatctttta
    27001 gctgggcctt tgtttcgtga cccatatcac aaaatcaagc atacactaat tattgaacta
    27061 attaatacca cgctaactgt ttcagttgat actaagtgtt aaaaatgtga attttaataa
    27121 caaaaaagta taaaaaacac ggttatttat tattaattat agtattttca attagataat
    27181 gaattttgca taatgtgctt tttttaccgt atgtattcat tttgtgaatg atgtgtcgcg
    27241 gtaaaacgca ctattcgtta aatgtatcgc aggagaaaga aggggaatga gagggataag
    27301 caacattttc cccgccgcca gaagcgacgg ggcagagatt aaagctcctg gtcgaacagc
    27361 tctaaaatcg cttcgtacag gtctttgacg gtgaaaccgt tagcaggggt ggtaaagatg
    27421 gtgtcatcgc cagcgatggt gcccagaata ccttctgctt tgcccagtga gtccagcagg
    27481 cgagcaatta actgcgccgc gccagggctg gtatgaatca cgacaactgc atcgttgtag
    27541 tcgatatcca gcaccagatt cttcaatgga ctggaggtgg ttggtacacc cagttcagct
    27601 ggcaggcagt aaaccatttc cattttggca ttgcgtgtac gtacagcacc aaacttggtc
    27661 aacatccgcg agactttaga ctgattaata ttgtcaaagc cttgctcctg caacgcggcg
    27721 acgatttcgc cctgggagct aaatttctct tctttaagta atgctttaaa tgctttaact
    27781 agttcttctt gcttagccga gcttcgcata agtcacccga tatggtggtt gatacaacat
    27841 tattgtgcat acagatgaat ttttatgcaa acagtcagcc ctgaagaagg ctgaaataat
    27901 gttatgaaag agcgggattt tatcaaattt cgttattgag aaacatgcct gcgtcacggc
    27961 atgcaaattc tgtttaaaag taaattaatt gttatcaaat tgatgttgtt ttggctgaac
    28021 ggtagggtat attgtcacca cctgttggaa tgttgcgcta atgcataagc gactgttaat
    28081 tacgtaagtt aggttcctga ttacggcaat taaatgcata aacgctaaac ttgcgtgact
    28141 acacattctt gagatgtggt cattgtaaac ggcaattttg tggattaagg tcgcggcagc
    28201 ggagcaacat atcttagttt atcaatataa taaggagttt aggatgaaag tcgcagtcct
    28261 cggcgctgct ggcggtattg gccaggcgct tgcactactg ttaaaaaccc aactgccttc
    28321 aggttcagaa ctctctctgt atgatatcgc tccagtgact cccggtgtgg ccgtcgatct
    28381 gagccatatc cctactgctg tgaaaatcaa aggtttttct ggtgaagatg cgactccggc
    28441 gctggaaggc gcagatgtcg ttcttatctc tgcaggcgta gcgcgtaaac cgggtatgga
    28501 tcgttccgac ctgtttaacg ttaacgccgg catcgtgaaa aacctggtac agcaagttgc
    28561 gaaaaactgc ccgaaagcgt gcattggtat tatcactaac ccggttaaca ccacagttgc
    28621 aattgctgct gaagtgctga aaaaagccgg tgtttatgac aaaaacaaac tgttcggcgt
    28681 taccacgctg gatatcattc gttccaacac ctttgttgcg gaactgaaag gcaaacagcc
    28741 aggcgaagtt gaagtgccgg ttattggcgg tcactctggt gttaccattc tgccgctgct
    28801 gtcacaggtt cctggcgtta gttttaccga gcaggaagtg gctgatctga ccaaacgtat
    28861 ccagaacgcg ggtactgagg tggttgaagc gaaagccggt ggcgggtctg caaccctgtc
    28921 tatgggccag gcagctgcac gttttggtct gtctctggtt cgtgcactgc agggcgaaca
    28981 aggcgttgtc gaatgtgcct acgttgaagg cgacggtcag tacgcccgtt tcttctctca
    29041 accgctgctg ctgggtaaaa acggcgtgga agagcgtaaa tctatcggta ccctgagcgc
    29101 atttgaacag aacgcgctgg aaggtatgct ggatacgctc aagaaagata tcgccctggg
    29161 cgaagagttc gttaataagt aattgattag cgaataataa aaaaccggag cacagactcc
    29221 ggtttttgtt ttgagcactc gacttaattg gttgccggat attcctgaat ggtgacctgc
    29281 agcgttaact gcttatcatc acgcatcact acaaccggga tcaccgaacc agggcgaatt
    29341 tccgccacct gatccatcgt ctccagagca gagatggccg gtttgttatc caccgaaata
    29401 atcagatcgt tgacctgaat acccgcattc gccgctgggc cgtcaggtga cacttcatta
    29461 accacgatcc cttgcagttg atctatacca ccgctctgcg cgtgcagtgg tgcgatctcg
    29521 cgtccgccga taccaatgta gccgcggatc acgcgaccat cgcggatcag cttatccata
    29581 attttggttg ctaactggaa aggaatcgca aagccgatac cttccggcgt ttcgccatcg
    29641 ttactcttat caaacgacag cgtgttaatg cccatcagtt ctcccagcga gttcaccagc
    29701 gcgccgccag agttaccgtg gttaatggaa gcatcggttt gtaggaagtt ttgccgcccg
    29761 gtcgggttca gaccgattcg acccgtggca ctaataatcc cttgggtaat ggtctgcccg
    29821 aggttgtacg ggttaccgat cgccagtact acgtcgccaa tgtgcggtac gcgacgtgca
    29881 ttaattggaa tggtaggtaa accgccagtg gcattaattt taagtaccgc cagatcggtt
    29941 agagagtcag atcccaccag caatgcttca aatacacgtc catcctgtaa ggcgacgatg
    30001 atctgatcgg cgtcgttgat gacgtgttta ttggtgatga tataaccgcg ttgatccatg
    30061 attacaccgg atcccagggt gcggatctca agctggttgt gagagttggt gttcaaacca
    30121 cggttgtaaa cgttaaccac cgctggcgcg gcgcggcgaa ccgccagatt atagctggca
    30181 ggcgtctcat cggtactgtc aaattgcgga gtggaaagcg ggttaaggct gcgcagcgaa
    30241 ggcatggcaa ccagcagaat agcgccgaca attaatccaa tcgcaacgga acgtaagagc
    30301 ttcacaaaca tgatggaggc gtcattaaaa aagggaacgg cagcagcata ccacgagtta
    30361 accggacatc acacgtcagc ctgatgcccg gtttacgaca ttaacgcatc agcagataga
    30421 tgctttcatt gccgcgtaca atttgcaggg cgatgatggc cggttttgcc gccagcactt
    30481 tacgcatttc agcaatcgag ttcacccgat cgcggttgac gccaatgatc acatcgtctt
    30541 tttgcaagcc agcctgagca gctgggcttc ctttgacaac ttcatctatc ttaatacctt
    30601 tgccgccatc ttttagctga ccatcgctca acgttgcacc ttccagcgct ggcgtgatca
    30661 tttcagcgct ggccgacgaa gaggtgttgg tatcaagcgt cacttctact tccagtggtt
    30721 tgccgttacg cagcaggcca agcttcactt tcgtgcccgg ctctgtagtc gcgatacgag
    30781 agcgcaactc agcaaagcta ttcagcggtt tgccgttgag gctggtaata atatcgcccg
    30841 ctttgacgcc cgctttcgct gagccagaac ttggcaacac ttcgctgaca aacgcgccac
    30901 gctgcacgtc aaggttgaag gctttggcga tatcggcact catctcggtg cctttgatgc
    30961 ctaacaaacc gcgtttgatt tcaccaaagt cgataagctg ctgcgccagt gttcgcgcca
    31021 tattactggg gatggcaaat ccaatcccga cgctcccgcc gccaggcgca aggattgcag
    31081 tgttgatgcc aattaactca ccgttaaggt ttaatagtgc accgccggag ttaccgcggt
    31141 taatggaagc atctgtctgg ataaagtttt ccagaccttc aagattcaac ccgctgcggc
    31201 ctaatgcgga aacaatgcca gaggtggcgg tttgcccaag gccaaatggg ttaccgaccg
    31261 ctacggcaaa atcaccgacg cgcaatttat cggagtcggc aatagcgatt tgcgttaatt
    31321 tgctcgggtt ttgaatttgt aacagggcga tatcgctctg gtcatcgcta ccaatcagtt
    31381 tttcatcaaa ctcgcgccca tcattgagct gaatactgat tttctgtgcc tgattaatca
    31441 catggttgtt ggtcagcaca tagcctttac tggcgttgat gatgacaccg gaacctaaac
    31501 cttcgaaggg ttgtgcaggt tgatccggta aatcatcacc aaaaaacttt ttgaattctt
    31561 ccgggatttt ctgtccctga ctggccgttc cttccacccg tacgctcacc actgccggaa
    31621 gcactttttc cagcattgga gccagactgg ggagaggggc ctgatcggca acctggcctg
    31681 gaatcgacgc gacggcctga aatgacgccg agagagttaa cccggcactt aacgctaatg
    31741 cactcaacag ctgggtttgt tttttcatta ttcctgctct cgtacctgaa tgataagaaa
    31801 agagattcaa aacagtttga tgttattgaa ttttcagacg gctaacaatg agacaccaga
    31861 ttaaatgata gtcgtggagg gagagaagag gagggcgcaa tggctgcgcc cgaaaaataa
    31921 attagtcgcg cttcgcgcca gtacgcagca ggccggatgc gccttcagaa tagtcgcgag
    31981 gcatctgcac cggtgcctga tcgttgctgg cttcagactc tgccagacga ttacggaacg
    32041 ggtttgcttc agcagacagt tccggcagca ggctgctgga gctttttgcc atgtgctgat
    32101 acagctggcg atagtcgtgc gccatggtat ccagtaattc cgcgctgcgg gcaaagtggc
    32161 taaccagctc ttcgcgatac tcgtccagtt cagctttatt cttttccagt tcgtactgca
    32221 acgcctgttg ctggcgtagt ttacgattac caaaacgcat ggccacagca ccaataatga
    32281 tgccgacgac taacccaatt agcgcatatt cccaggtcat gaacatctcc cgttgtcttt
    32341 tgtttccgta gggtgttggc ttcaggctcc atgcctgcgg ctgattatgc cactataacc
    32401 gttaattcca cagaagtgga atcccgactg catatcgcgt agtgtagaac ggcctttttt
    32461 tcgtcaaccg tgaacaacgg cgcaccgatt attcaaggaa taacaataag atcatgcaaa
    32521 gcgttacccc aacatcgcaa tacctgaagg cgctcaatga aggcagccat caacccgacg
    32581 acgttcaaaa agaggccgtc agccgcctgg aaattattta tcaagaactc atcaatagca
    32641 ggccaccagc ccccaggacg agtgggctaa tggcgcgggt cggtaagctg tggggtaaac
    32701 gcgaagacac aaagcatacg ccagtgcgtg gcttatatat gtggggcggt gtaggacgcg
    32761 ggaaaacctg gctgatggac cttttctatc aaagcctgcc gggagagcgg aaacagcgcc
    32821 tgcactttca ccgttttatg ctgcgggtgc acgaagagct aactgcctta caggggcaga
    32881 ccgatccgct ggaaattatt gccgatcgct ttaaagccga aactgacgtg ctctgttttg
    32941 acgaattttt tgtttctgat attaccgacg ccatgctact tggcggtctg atgaaagccc
    33001 tgttcacccg cggtattacc ctggtagcga cgtcaaatat tccgccggac gaactttatc
    33061 gaaatggcct gcaacgtgcg cgttttctgc ctgcaatcga tgccattaaa cagcattgtg
    33121 atgtaatgaa cgtggacgct ggtgttgatt atcgactgcg tacactcact caggcgcatc
    33181 tgtggctttc gccactcaac gatgaaaccc gggcgcagat ggataaacta tggttggcgc
    33241 tggcgggggt gaaacgagaa aattcaccga cgttagaaat caaccatcgg ccattggcga
    33301 caatgggcgt cgagaaccag acgctggcgg tttcttttac tacgctgtgc gtcgacgccc
    33361 gcagtcagca tgactatatt gcgctctcac gcctgtttca cacagtaatg ttgtttgatg
    33421 tgccagtcat gacgcggttg atggagagcg aagcgcggcg ctttattgcg ctggtggatg
    33481 agttttacga gcgccatgtc aaattagtgg tgagtgcaga agtgccgctg tatgaaattt
    33541 atcagggtga gcggctgaag tttgagttcc agcgttgcct gtcacgtctg caagagatgc
    33601 aaagcgaaga gtatctgaag cgcgagcatt tggcgggtta aaacctgcca caaatcacaa
    33661 aaaggggtcg atctttgacc ccgacttctc tataatcctg cgaccccacg ttacaagaaa
    33721 gtttttttcc caaaactttt tgtgtgctgg cataggctat tcgaaggggt aggtttgccg
    33781 gactttgtcg tgtgaacctc aacaattgaa gacgtttggg tgttcaccaa cgtgtaacta
    33841 tttattgggt aagcttttaa tgaaaacttt tacagctaaa ccagaaaccg taaaacgcga
    33901 ctggtatgtt gttgacgcga ccggtaaaac tctgggccgt ctggctactg aactggctcg
    33961 tcgcctgcgt ggtaagcaca aagcggaata tactccgcac gtagataccg gtgattacat
    34021 catcgttctg aacgctgaca aagttgctgt aaccggcaac aagcgtactg acaaagtgta
    34081 ctatcaccac accggccaca tcggtggtat caaacaagcg acctttgaag agatgattgc
    34141 tcgccgtcct gagcgtgtga ttgaaatcgc ggttaaaggc atgttgccaa aaggcccgct
    34201 gggtcgtgct atgttccgta aactgaaagt ttacgcgggt aacgagcaca accacgcggc
    34261 acagcaaccg caagttcttg acatctaatc gggattatag gcaatggctg aaaatcaata
    34321 ctacggcact ggtcgccgca aaagttccgc agctcgcgtt ttcatcaaac cgggcaacgg
    34381 taaaatcgta atcaaccaac gttctctgga acagtacttc ggtcgtgaaa ctgcccgcat
    34441 ggtagttcgt cagccgctgg aactggtcga catggttgag aaactggacc tgtacatcac
    34501 cgttaaaggt ggtggtatct ctggtcaggc tggtgcgatc cgtcacggta tcacccgcgc
    34561 tctgatggaa tacgacgagt ccctgcgttc tgaactgcgt aaagctggct tcgttactcg
    34621 tgacgctcgt caggttgaac gtaagaaagt cggtctgcgt aaagcacgtc gtcgtccgca
    34681 gttctccaaa cgttaattgg cttctgctcc ggcagaaaac aatttgcgaa aaaacccgct
    34741 tcggcgggtt tttttatggc taaattctga atcagcgtaa aaaatgggaa gttgcttttt
    34801 gctgccgtct gaaagacagg taaaacaaaa accacatcgc caataagggt ctaagtcaac
    34861 tatttcagac taaagcgcat ctctttttcc ccatttccgg catccactca ccacaaaggt
    34921 cgcaaaatct ggtaaactat catccaattt tctgcccaaa tgtcgggtat tgctcatttt
    34981 ttgtttgatt ttcgaacaaa gagagtcgtt ccttattggg taacacaact tctgactggc
    35041 cacctggtgg ctggtagcag taaaaattct gactatacct ggaggttttc atggctgtcg
    35101 ctgccaacaa acgttcggta atgacgctgt tttccggtcc tactgacatc tatagccatc
    35161 aggtccgcat tgtgctggct gagaaaggtg taagtttcga gatcgaacac gtggaaaagg
    35221 acaatccgcc tcaggatctg attgacctta acccgaatca gagcgttccg accctggtgg
    35281 atcgtgagct gaccctgtgg gaatctcgca tcattatgga atatctggat gagcgtttcc
    35341 cgcatccgcc actgatgcct gtttacccgg tagctcgcgg tgaaagccgt ctgtacatgc
    35401 atcgcatcga aaaagactgg tacacgctga taaacactat catcaacggt tcagcttctg
    35461 aagcagatgc cgcacgtaag caactgcgcg aagaactgct ggcgattgcg ccggtcttcg
    35521 gtcagaagcc gtacttcctg agcgatgagt tcagcctggt cgattgctac cttgctccgc
    35581 tgctgtggcg tctgccgcaa ctgggcatcg agttcagcgg cccgggtgcg aaagagctga
    35641 aaggctatat gacccgcgtc tttgagcgtg actctttcct tgcttcttta actgaagcag
    35701 aacgtgaaat gcgtctgggc cggagttaat ctgtatggat ttgtcacagc taacaccacg
    35761 tcgtccctat ctgctgcgtg cattctatga gtggttgctg gataaccagc tcacgccgca
    35821 cttggtggtg gatgtgacgc tccctggcgt gcaggttcct atggaatatg cgcgtgacgg
    35881 gcaaatcgta ctcaacattg cgccgcgtgc tgtcggcaat ctggaactgg cgaatgatga
    35941 ggtgcgcttt aacgcgcgct ttggtggcat tccgcgtcag gtttctgtgc cgctggctgc
    36001 cgtgctggct atctacgccc gtgaaaatgg cgcaggcacg atgtttgagc ctgaagctgc
    36061 ctacgatgaa gataccagca tcatgaatga tgaagaggca tcggcagaca acgaaaccgt
    36121 tatgtcggtt attgatggcg acaagccaga tcacgatgat gacactcatc ctgacgatga
    36181 acctccgcag ccaccacgcg gtggtcgacc ggcattacgc gttgtgaagt aatacaaaac
    36241 aggcccaggc ggcctgtttt gtctttttaa tgacttaata aaatagcgtg attgtggcaa
    36301 tcatattcac cacgaaaccg actgccatgg ggatcgctgt ccgcttcacc accgcaaaag
    36361 gcgaaacgcc tgcaattcct gacgtaacga ccaccaccgc agtaatcggc gaaaccgcgc
    36421 gcgctagcgt cgtggcaaaa tgcatcggca taatcattac aaccgctggt acatgtagtc
    36481 cggctgcgat attcggaata agactggcaa atgacataaa cggcgcattg ccagagccca
    36541 tcacaatggc acaaatggca atgactagcg ccataataat catcacgcca ataccgccca
    36601 gaccagaatg ctccgcaccc ctgataaccg catcaacagt gccaatcgtc gttaagcctt
    36661 tcgcaaatat ttcacccgcg accaccagcg ttaccacgtt gacaaactgc gtacccatgc
    36721 cgtcaaaaaa cgcctgcaca tcgtccattg tttcgcgcaa gttatgcttg cggaagaact
    36781 caacaaacat cgtcaccgtc aaacttagta acatcaccac caccagatgc agttctgact
    36841 tcatcagccc gacgtgggcg aggaacagcg agccgagcat caggattaac ggcatcacag
    36901 gtaaaatggc gtaatagagc ggcgggacat tatcgagagt tttttgctct gcctgttcgt
    36961 gattgatatt tttatctttt ttgtcaaaag cgcgttgcac gaaaaagtgg gagatcgcca
    37021 ccgagataat gacgcaagag gcgaccggaa gctggtagtg gaagaagtat gtggcaattt
    37081 tcattcccgc gacctgggct gcaaaaatgg agttcgtttc cagaatcccc cactcaatgg
    37141 acatcgtggt tgcgataacc gccaccgcag agaggcgact tactcccaga ctcaccagcg
    37201 tcggaaataa ggtgaccatc agcaacatac ccagaccgga ggcgctggta ataaactgcg
    37261 ccatgatttg gccgatgacg taagttgccg acagaataat atacggcgag cgaatgagtt
    37321 ttaacgggcg gcttagcaag ctcaccatcg cgcgactggc tcctatgcgc tccatgtagc
    37381 gggcataacc gcccaccgcc ataatcgaca gccccaatcc ggcaatgcgg ttggacagca
    37441 tgcgcaggat ttcattataa atgtcgacga tcaagtagcc gctgcttttg gcgggatcga
    37501 gtacaccacc gaaccctaac cagactccgc acatcatcag gaagacacct gcggcagcca
    37561 gcaccacctg aggtttgtag tttttcagga tcaaatagcc catcgtaatt aatacgatga
    37621 cagatataat
//
LOCUS       DAJTNM010000021        36638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTNM010000021 DAJTNM010000000
VERSION     DAJTNM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.021"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(156..1391)
                     /locus_tag="OO172_001234"
     CDS             complement(156..1391)
                     /locus_tag="OO172_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5511490.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OO172_001235"
     tRNA            complement(1538..1614)
                     /locus_tag="OO172_001235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="OO172_001236"
     tRNA            complement(1657..1743)
                     /locus_tag="OO172_001236"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="OO172_001237"
     tRNA            complement(1764..1839)
                     /locus_tag="OO172_001237"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="OO172_001238"
     tRNA            complement(1898..1974)
                     /locus_tag="OO172_001238"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="OO172_001239"
     CDS             complement(2077..3462)
                     /locus_tag="OO172_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5511491.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO172_001240"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO172_001240"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5511492.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO172_001241"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO172_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5511493.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO172_001242"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO172_001242"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5511494.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO172_001243"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO172_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5511495.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRFGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO172_001244"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO172_001244"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5511496.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO172_001245"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO172_001245"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5511497.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO172_001246"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO172_001246"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5511498.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO172_001247"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO172_001247"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5511499.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO172_001248"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO172_001248"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5511500.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO172_001249"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO172_001249"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5511501.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAVNYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO172_001250"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO172_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5511502.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO172_001251"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO172_001251"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5511503.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO172_001252"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO172_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5511504.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO172_001253"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO172_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5511505.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO172_001254"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO172_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5511506.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="OO172_001255"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="OO172_001255"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5511507.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="OO172_001256"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="OO172_001256"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5511508.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO172_001257"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO172_001257"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5511509.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAVKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="OO172_001258"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="OO172_001258"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5511510.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO172_001259"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO172_001259"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5511511.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="OO172_001260"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="OO172_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5511512.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO172_001261"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO172_001261"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5511513.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO172_001262"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO172_001262"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5511514.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO172_001263"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO172_001263"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5511515.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO172_001264"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO172_001264"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5511516.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="OO172_001265"
     CDS             complement(31267..32913)
                     /locus_tag="OO172_001265"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5511517.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="OO172_001266"
     CDS             complement(32916..32966)
                     /locus_tag="OO172_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511518.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO172_001267"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO172_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5511519.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="OO172_001268"
     CDS             33502..35022
                     /locus_tag="OO172_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR5511520.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="OO172_001269"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="OO172_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5511521.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="OO172_001270"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="OO172_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5511522.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="OO172_001271"
     tRNA            complement(36439..36514)
                     /locus_tag="OO172_001271"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
     gene            complement(36523..36599)
                     /locus_tag="OO172_001272"
     tRNA            complement(36523..36599)
                     /locus_tag="OO172_001272"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36563..36565),aa:Asp,seq:gtc)
BASE COUNT         8946 a        10031 c         9230 g         8431 t
ORIGIN      
        1 gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccgac
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gccatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttatgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacgcc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccaacccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cacgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcataacggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgccaaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcaac tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag tcaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattca gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaattta ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagatcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctctcga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 gctgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag tcctgtgcaa aaattaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcgttagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttaccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcatgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgggc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatggttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca cgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattct ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtggtgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggagcacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggct attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgct ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggag tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcata gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgttcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgctat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aaggggggcg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag ggtggcggaa cggacgggac
    36541 tcgaacccgc gaccccctgc gtgacaggca ggtattctaa ccgactgaac taccgctcca
    36601 ccgaattctt ttacaaccac cggttttatg accggctt
//
LOCUS       DAJTNM010000022        36607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTNM010000022 DAJTNM010000000
VERSION     DAJTNM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.022"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            27..2294
                     /gene="adiA"
                     /locus_tag="OO172_001273"
     CDS             27..2294
                     /gene="adiA"
                     /locus_tag="OO172_001273"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5511523.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2619..3380
                     /gene="adiY"
                     /locus_tag="OO172_001274"
     CDS             2619..3380
                     /gene="adiY"
                     /locus_tag="OO172_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5511524.1"
                     /translation="MRICSDQPCIVLLTEKDVRIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3517..4854
                     /gene="adiC"
                     /locus_tag="OO172_001275"
     CDS             3517..4854
                     /gene="adiC"
                     /locus_tag="OO172_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5511525.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4958..6601
                     /gene="eptA"
                     /locus_tag="OO172_001276"
     CDS             4958..6601
                     /gene="eptA"
                     /locus_tag="OO172_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5511526.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6598..7266
                     /gene="basR"
                     /locus_tag="OO172_001277"
     CDS             6598..7266
                     /gene="basR"
                     /locus_tag="OO172_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5511527.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7267..8367
                     /gene="pmrB"
                     /locus_tag="OO172_001278"
     CDS             7267..8367
                     /gene="pmrB"
                     /locus_tag="OO172_001278"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5511528.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="OO172_001279"
     CDS             complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="OO172_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5511529.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8544..10046)
                     /gene="proP"
                     /locus_tag="OO172_001280"
     CDS             complement(8544..10046)
                     /gene="proP"
                     /locus_tag="OO172_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5511530.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10310..11188)
                     /locus_tag="OO172_001281"
     CDS             complement(10310..11188)
                     /locus_tag="OO172_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5511531.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNSGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="OO172_001282"
     CDS             complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="OO172_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5511532.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13815..14150
                     /locus_tag="OO172_001283"
     CDS             13815..14150
                     /locus_tag="OO172_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5511533.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14308..14751
                     /gene="yjdN"
                     /locus_tag="OO172_001284"
     CDS             14308..14751
                     /gene="yjdN"
                     /locus_tag="OO172_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5511534.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14884..15672
                     /gene="phnC"
                     /locus_tag="OO172_001285"
     CDS             14884..15672
                     /gene="phnC"
                     /locus_tag="OO172_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5511535.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15697..16713
                     /gene="phnD"
                     /locus_tag="OO172_001286"
     CDS             15697..16713
                     /gene="phnD"
                     /locus_tag="OO172_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5511536.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSSGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16819..17598
                     /gene="phnE"
                     /locus_tag="OO172_001287"
     CDS             16819..17598
                     /gene="phnE"
                     /locus_tag="OO172_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR5511537.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17619..18344
                     /gene="phnF"
                     /locus_tag="OO172_001288"
     CDS             17619..18344
                     /gene="phnF"
                     /locus_tag="OO172_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR5511538.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18345..18797
                     /gene="phnG"
                     /locus_tag="OO172_001289"
     CDS             18345..18797
                     /gene="phnG"
                     /locus_tag="OO172_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR5511539.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMIRGDNA"
     gene            18794..19378
                     /gene="phnH"
                     /locus_tag="OO172_001290"
     CDS             18794..19378
                     /gene="phnH"
                     /locus_tag="OO172_001290"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR5511540.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19378..20442
                     /gene="phnI"
                     /locus_tag="OO172_001291"
     CDS             19378..20442
                     /gene="phnI"
                     /locus_tag="OO172_001291"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR5511541.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20435..21280
                     /gene="phnJ"
                     /locus_tag="OO172_001292"
     CDS             20435..21280
                     /gene="phnJ"
                     /locus_tag="OO172_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR5511542.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21277..22035
                     /gene="phnK"
                     /locus_tag="OO172_001293"
     CDS             21277..22035
                     /gene="phnK"
                     /locus_tag="OO172_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR5511543.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22146..22826
                     /gene="phnL"
                     /locus_tag="OO172_001294"
     CDS             22146..22826
                     /gene="phnL"
                     /locus_tag="OO172_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR5511544.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22823..23959
                     /gene="phnM"
                     /locus_tag="OO172_001295"
     CDS             22823..23959
                     /gene="phnM"
                     /locus_tag="OO172_001295"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR5511545.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPTSLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            23959..24516
                     /gene="phnN"
                     /locus_tag="OO172_001296"
     CDS             23959..24516
                     /gene="phnN"
                     /locus_tag="OO172_001296"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR5511546.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLLVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24503..24937
                     /gene="phnO"
                     /locus_tag="OO172_001297"
     CDS             24503..24937
                     /gene="phnO"
                     /locus_tag="OO172_001297"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR5511547.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24939..25697
                     /gene="phnP"
                     /locus_tag="OO172_001298"
     CDS             24939..25697
                     /gene="phnP"
                     /locus_tag="OO172_001298"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR5511548.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRAQRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(25844..26173)
                     /locus_tag="OO172_001299"
     CDS             complement(25844..26173)
                     /locus_tag="OO172_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511549.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26242..26874)
                     /locus_tag="OO172_001300"
     CDS             complement(26242..26874)
                     /locus_tag="OO172_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5511550.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGCCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26955..29170)
                     /locus_tag="OO172_001301"
                     /pseudo
     CDS             complement(26955..29170)
                     /locus_tag="OO172_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            29284..30804
                     /locus_tag="OO172_001302"
     CDS             29284..30804
                     /locus_tag="OO172_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5511551.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30801..31775
                     /locus_tag="OO172_001303"
     CDS             30801..31775
                     /locus_tag="OO172_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5511552.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            31806..32747
                     /locus_tag="OO172_001304"
     CDS             31806..32747
                     /locus_tag="OO172_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5511553.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            32802..33485
                     /locus_tag="OO172_001305"
     CDS             32802..33485
                     /locus_tag="OO172_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HCR5511554.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQRLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            33500..34360
                     /locus_tag="OO172_001306"
     CDS             33500..34360
                     /locus_tag="OO172_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HCR5511555.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            34357..35358
                     /locus_tag="OO172_001307"
     CDS             34357..35358
                     /locus_tag="OO172_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5511556.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(35355..36061)
                     /locus_tag="OO172_001308"
                     /pseudo
     CDS             complement(35355..36061)
                     /locus_tag="OO172_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            36352..>36607
                     /locus_tag="OO172_001309"
     CDS             36352..>36607
                     /locus_tag="OO172_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511557.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYY"
BASE COUNT         8529 a        10228 c         9717 g         8133 t
ORIGIN      
        1 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
       61 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
      121 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
      181 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
      241 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
      301 gcgatcggga aaaagccctc gccgcaatgg accgggatct gctggagctt gtcgatgaat
      361 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt gccgcgatga
      421 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagtgaca
      481 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
      541 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gacatgggca
      601 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
      661 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtacttccg
      721 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
      781 gtaactgcca taaatccatc gaacaaggtt tgatgctgac aggcgcgaaa ccggtctata
      841 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
      901 ctgaaacctt gcaaaagaaa atcagtgaaa gcccgctgac caaagacaaa gccgggcaaa
      961 aaccgtctta ctgcgtggta accaactgca cctatgacgg cgtgtgttat aacgctaaag
     1021 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     1081 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     1141 atcacaacgg tcctaccgtt ttcgccaccc actccatcca caaactgctg aatgcgctgt
     1201 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     1261 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     1321 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacag gaagtgatta
     1381 acgaagcggt tgatttccgt caggcgatgg cgcggctata taaagagttc accgctgacg
     1441 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     1501 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     1561 tacatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     1621 acccaattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     1681 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     1741 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     1801 ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     1861 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     1921 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     1981 acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     2041 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     2101 actcagttat cccgtatccg ccaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     2161 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     2221 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     2281 gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     2341 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     2401 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     2461 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     2521 atgcatgttt atattcatca tataattata taaccatttc ccggtatcgc tttgctttag
     2581 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     2641 cttgtattgt tttattgact gaaaaagatg tccggataag ggtgaatggg aaagaaccta
     2701 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     2761 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gactacctcc
     2821 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     2881 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     2941 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     3001 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     3061 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     3121 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagttgaaa agcgaaaaca
     3181 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     3241 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     3301 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     3361 gagaacgcac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     3421 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     3481 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     3541 aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg gtcaggtgtt
     3601 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     3661 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgaccca
     3721 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     3781 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgccatggt ggtcattggc
     3841 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     3901 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     3961 cgcgtgcagg ccgttgccac tgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     4021 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     4081 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     4141 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     4201 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     4261 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     4321 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     4381 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     4441 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     4501 atcgtcggta ttttgatgac catcttccag ctcagcagca tttcaccaaa cgcgaccaaa
     4561 gagttcggtc tggtttcttc cgtctcggtc atctttacac tggtgccata tctttacacc
     4621 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     4681 gctgttacta ccattgcctt cctctactgc atttgggccg tggtggggtc cggggcgaaa
     4741 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     4801 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     4861 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     4921 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     4981 accctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     5041 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     5101 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     5161 aagctctttc ttatggctta atcgaacact ggcctgcctg tttattctgg ttggcgcggc
     5221 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     5281 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     5341 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     5401 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     5461 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     5521 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     5581 tcagcgactg gcaaatctgc cgctggtgcg aattggtgaa gacgcgcacc gcaacccgtt
     5641 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     5701 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     5761 cgtggtctat ttccctaata ccgcatcttg cggtacggca acggcagttt cagtaccgtg
     5821 catgttctcg gatatgccgc gtgagcacta caaagaagag ctggcacagc accaggaagg
     5881 cgtgctggat atcattcagc gagcgggcat caacgtgctg tggaatgaca acgatggcgg
     5941 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctga
     6001 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     6061 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     6121 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     6181 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     6241 cgactatatt gttgataaag cgattaatct gcttaaagaa catcaggata aatttaccac
     6301 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
     6361 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccaatgc tgctgtggct
     6421 gtcggaggat tatcaaaagc ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
     6481 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
     6541 gacgaagtat taccaggccg cggatgatat tctgcaaact tgcaggagag tgagtaaatg
     6601 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
     6661 accgaaggct acgcgtgcga tggcgtgaca accgcgcgga tggcggaaca aagccttgag
     6721 gacggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
     6781 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
     6841 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
     6901 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
     6961 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
     7021 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
     7081 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
     7141 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
     7201 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
     7261 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
     7321 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg gctatggcat
     7381 gaaagtaccg agcagattca actgtttgag caggcgctac gcgacaatcg caacaacgat
     7441 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
     7501 atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac ccgcccgctg
     7561 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
     7621 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
     7681 ctgaccagca cgctggataa cgaaaggttg tttaccgctg acgtcgcgca cgaactgcga
     7741 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
     7801 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
     7861 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
     7921 gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
     7981 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
     8041 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccgcaagg cagcaacatt
     8101 atgattaagc tgcaagaaga tggcggagcg gtcatggcag ttgaagatga aggaccaggt
     8161 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
     8221 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
     8281 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
     8341 gatcagaacg tggcaaacca gatataaaga aagctgctga ccaccagcac gctgaacacg
     8401 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
     8461 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
     8521 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
     8581 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat tatcgatttt ctgctcgata
     8641 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
     8701 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
     8761 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
     8821 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
     8881 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
     8941 ataaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
     9001 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagtgcaac actaccaagt
     9061 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     9121 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     9181 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     9241 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     9301 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     9361 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     9421 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     9481 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     9541 tccagccagc tgcccataaa gccacgttta cggtcggggg agtattccgc aacaaatatc
     9601 gacgccccgg tatattcacc accgaccgag aaaccttgtg ccatcttaca gatcaacagc
     9661 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     9721 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
     9781 aacataccaa agaagagtcc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
     9841 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
     9901 taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc cagtgatgct
     9961 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    10021 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttgaccca tttccgcaaa
    10081 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    10141 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    10201 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    10261 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    10321 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    10381 gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg ggttgtttat
    10441 ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg cccgtcggaa
    10501 cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
    10561 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagcaacaac accaccgctt
    10621 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
    10681 taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa
    10741 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
    10801 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
    10861 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
    10921 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
    10981 gcagactaag cggcgcattt aacatgccgc tgttcgtcca tgtctgaagc tgcacacgcg
    11041 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttga ggatggcggg
    11101 catcatcgcc ctgcgccaga tccactaaaa agcgcggatg aaccgactcc agcacgcgac
    11161 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
    11221 atcgcgtaac agctgagcgt cggtgtaaat ccagctgtga gtcatcacag tctgctgcaa
    11281 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca gactttcgcg
    11341 taaacgcgtc tgtaattctg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
    11401 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaatagag
    11461 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
    11521 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
    11581 aacgcctaaa cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga cttcactgaa
    11641 cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg
    11701 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc
    11761 ggttaacacc cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact
    11821 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct cgcttaacgg
    11881 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc accgcatctt gctgttgacg
    11941 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
    12001 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
    12061 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
    12121 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
    12181 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
    12241 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
    12301 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
    12361 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
    12421 gaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
    12481 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
    12541 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    12601 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    12661 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    12721 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    12781 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    12841 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    12901 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    12961 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    13021 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    13081 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    13141 accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag
    13201 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc
    13261 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    13321 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    13381 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    13441 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
    13501 agaaataatt ttatatttag tgtgttgttt tttatcagaa taaataacgt cttctgatgc
    13561 gtttaaaacg tcagaaagat aaaaatatca tgtaaataaa aaaaagaaca agtagagcat
    13621 taacattatc ttaaataata aatagaggca aaaagattat tttctttttg cgtttccttt
    13681 caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt
    13741 gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt
    13801 ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacttacg
    13861 aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg
    13921 cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg
    13981 acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg ctgaaaattg
    14041 gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg
    14101 acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga ttgtattgtg
    14161 atcggtaagc cgaataaggc gctcgcgccg catccggcaa cgatgccgac tgcctgatgc
    14221 gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac tacacttaac
    14281 tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt
    14341 aactgttccg acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa
    14401 atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg ccgagaactg cccttccgga
    14461 atgcaatttc ccgataccgc catcgctcat gccaacgtgc gcattgccgg aagcgacatc
    14521 atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac gctggtgctc
    14581 gattcgcaac aggtcgaaga aggaaaacgc tggtttgata accttgccgc taacggaaaa
    14641 atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa
    14701 tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata acccgccggg
    14761 aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt
    14821 taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc
    14881 atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaagcg
    14941 ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg tggctctgct tgggccgtcg
    15001 ggttccggca aatccaccct tttacgtcat ttaagcggtt tgattaccgg cgataaatcc
    15061 gccggtagcc atatcgagct actgggccgc actgtccagc gcgaaggccg cctggcccgc
    15121 gatatccgca aaagccgcgc caacaccggc tacatctttc aacaattcaa cctggtgaac
    15181 cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc gttctggcgc
    15241 acctgtttta gctggttcac cggcgagcag aaacagcgcg cgttacaggc gctgacccgc
    15301 gttggcatgg tgcattttgc ccatcagcgc gtttccaccc tctccggcgg ccagcagcaa
    15361 cgtgtggcga ttgcccgtgc gctgatgcag caggcgaaag tgattctggc cgatgaaccc
    15421 atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg acaccctgcg cgacatcaac
    15481 cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc cctgcgctac
    15541 tgcgaacgca tcgtcgccct gcgtcagggg cacgtcttct acgacggcag cagccaactg
    15601 tttgataacg aacgttttga ccatctctac cgcagcatta accgcatcga agagaacgcg
    15661 aaagctgcct gatatcccca tcattgagga aaacgaatga acgctaagat aattgcctcg
    15721 ctggccttca ccagcatgtt cagcctcagc accctgttaa ccccggcaca cgccgaagag
    15781 caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa cctgaaaccg
    15841 caatggacgc cgtttttaca ggatatggag aagaaactgg gcgtgaaggt caacgccttc
    15901 tttgccccgg actacgcggg cattatccag gggatgcgct tcaataaagt ggatattgcc
    15961 tggtacggca acctgtcggc gatggaagcg gtggatcgcg ccaatggcca ggtcttcgcc
    16021 cagacggtcg cggcggatgg atcgtcgggt tactggagcg tgttgatcgt caacaaagac
    16081 agcccgatca acaacctgaa cgatctgctg gcgaagcgga aagatctcac cttcggcaat
    16141 ggcgatccta actccacctc tggcttcctc gtccccggtt actacgtctt cgctaaaaac
    16201 aatatctccg ccagcgactt caagcgcacc gtcaacgccg ggcatgaaac caacgcgctg
    16261 gccgtcgcca acaagcaggt ggatgttgcc accaacaaca ccgaaaacct cgacaagctg
    16321 aaaacctccg cgccggagaa gcttaaagag ctgaaggtga tctggaaatc gccgctgatc
    16381 ctaggcgatc cgatcgtctg gcgcaagaat ctgtccgaaa ctaccaaaga caagatctac
    16441 gacttcttta tgaactacgg aaaaacgccg gaagagaaag cggtgctgga acgcctaggc
    16501 tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca gctcgcactg
    16561 tttaaagaga tacaaagcgt gaaaggcaat aaagggctga atgagcagga caagctggcg
    16621 aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa cgcgttaagc
    16681 gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag acgccccggc
    16741 gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc ttatccggcc
    16801 tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg tagctggttc
    16861 tcgcttctga gctgggccgt tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa
    16921 atggccccgc ttacgctgat taaagacggc ggcaacatgg caaccttcgc tgccgacttc
    16981 ttcccacccg atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa
    17041 atcgccgtct ggggcaccgc gctggcagtg gttctctcca tccccttcgg tctgatgagc
    17101 gccgaaaact tggtgccgtg gtgggtttac cagcccgttc gccgcctgat ggacgcctgc
    17161 cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg cctcggcccg
    17221 ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa gctgctttcc
    17281 gaagcggagg aagcgatcga acccggcccg gtggaaggca ttcgcgccac cggcgccaac
    17341 aaactcgaag agatccttta cggcgtgctg ccgcaggtga tgccgctgct gatctcctac
    17401 tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat ggtcggcgcg
    17461 ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc
    17521 gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc tcaacggttg
    17581 cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga
    17641 ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac
    17701 actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga
    17761 accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc
    17821 gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg
    17881 cgcgttttag ccagaacctg ctggaccagg gcagccatcc caccagcgaa aaactgctgt
    17941 cggtattgcg ccccgcgtcc ggtcacgttg ctgacgcgct ggggattacc gagggcgaga
    18001 acgttatcca tttgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt ttaatcgacc
    18061 actactttgc ggacctcacc ctttggccga cgctgcaacg cttcgacagc ggttcgctgc
    18121 acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg cggatcagcg
    18181 cccgccgcgc ccaggccaaa gagtgccagc gccttgaaat cccgaatatg tcgccgctgc
    18241 tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca
    18301 gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg
    18361 cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa ctggcagccc
    18421 gcctgaacgc gctgaacatc accgccgact atgaagtgat ccgcaccgcc gaaaccggcc
    18481 tggtacagat tcaggcgcga atgggcggca ccggcgaacg cttttttgcc ggcgacgcca
    18541 cgctgacccg cgccgccgta cgcctgactg acggcaccct cggctacagc tgggtgctgg
    18601 ggcgtgataa acagcacacc gaacgctgcg cgctgattga tgcgctgatg cagcaatctc
    18661 gccactttca aaacttatca gaaaccctta ttgccccgct ggacgctgac cgtatggcac
    18721 gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt acgatgattc
    18781 gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg atgcccagca
    18841 cagttttcgt cgcctgttaa aggccataag cgagccgggc gtgattgtcg ccctgcatca
    18901 gctcaagcgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc
    18961 tgataacgac acgccggtgt ggcttgccgc gccattgagt aacgatatcg tcaatcagag
    19021 cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga ccttcgcggt
    19081 gacggatgac ggaatttcca gcaaacagct caacgccctt tccaccggca ccgccgttgc
    19141 gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg ggcgcatgtt
    19201 gcgtctcacc ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc tgccggagtg
    19261 cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct
    19321 gacctgcggc gaacgcctgc tggctattcc gcgaaccact catgtggagg tgtgctgatg
    19381 tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc
    19441 cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccaaattga acagcagctt
    19501 aatctcgcgg tggatcgcgt gatgaccgaa ggcggcgttg ccgaccgcga actggcggcg
    19561 ctggcgctga aacaggccag cggcgataac gttgaagcaa ttttcctgct gcgcgcctac
    19621 cgcaccacgc tggcgaaact ggcggtaagc gagccgctcg acaccaccga gatgcgtctc
    19681 gaacggcgta tttcggcggt ttataaagac attcccggcg gccagctgct tggcccaacc
    19741 tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggtga agcgccgacg
    19801 ctgaccaccg ccgacagcga acaacagccg tcgccgcacg ttttcagcct gctggcgcgt
    19861 caggggctgg cgaagtttga agaagatagc ggcgcacagc cggatgacat cacccgcacg
    19921 ccgccggttt acccctgctc acgctcctcc cgcctgcaac aattaatgcg cggggacgaa
    19981 ggctatttgc tggcgctggc ctactccacc cagcgtggtt acggacgcaa tcacccgttc
    20041 gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga gctgggattt
    20101 gcggtaaacg tcggcgaact gctgatgacc gagtgtgaaa tggtcaacgg ttttatcgac
    20161 ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtattcgg catgagcgag
    20221 cgcaaagcga tggcaatggc gctggttgac cgtgcgttgc aagccccgga atacggcgag
    20281 cacgcgacag gcccggcgca ggatgaagag ttcgtgctgg cgcatgccga caacgtcgaa
    20341 gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca agccgaactg
    20401 gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt
    20461 tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa aagcggtggc
    20521 tatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccct acggctgggg
    20581 aaccggcggc attcagctca ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat
    20641 tgaccagggc gcagacgaca ccactaacgc cgtgtcgatt cgcaacttct tcaagcgcgt
    20701 gaccggggtg aacaccactg aacgtacgga cgatgcgacg cttatccaga cgcgccatcg
    20761 catccccgaa acgccgctga ccgaagatca gatagttatt ttccaggtgc cgatccccga
    20821 accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc actatgcacg cgctggagga
    20881 gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac
    20941 cacctatgct tatccggtga aggtgaacgg gcgctacgtg atggacccgt cgccgatccc
    21001 gaaattcgat aacccgaaaa tggacatgat gcccgccctg cagctgttcg gcgcagggcg
    21061 cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg atttcgacga
    21121 tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat cgacccacag
    21181 ctatctcgac gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac
    21241 cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttccgtc
    21301 aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc ttttgattta
    21361 tggccggggg aagtgctggg catcgtcggg gaatccggct ccgggaagac cacgctgctg
    21421 aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcgctacga gaatcgttcg
    21481 ttgtatgcga tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg
    21541 catcagcatc cactcgacgg cctgcgtcgc caggtctcgg caggcggcaa tatcggcgag
    21601 cggctgatgg cgaccggggc ccgtcattac ggcgacatcc gcgccaccgc gcagaagtgg
    21661 ctggaagagg tggagattcc cgccaaccgg atcgacgacc tgccgaccac cttttccggc
    21721 ggcatgcagc aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt
    21781 atggacgagc caaccggtgg gctggatgtg tcggtgcagg cacgcctgct cgatctactg
    21841 cgcggcctgg tggtggagct gaacctggcg gtggtgattg tcacccatga tttaggcgtc
    21901 gcccgcctgc tagcggaccg tttgctggtg atgaagcagg ggcaagtagt ggagagtggg
    21961 ttaaccgacc gcgtgctcga cgacccgcac catccgtata cccagctgct ggtgtcatcg
    22021 gttttgcaga attgagccgg tgccggatgc ggcgtaaacg ccttatctgg cctacaaatg
    22081 cgctccccgt aggtcggata agacgcatca gcgtcgcatc cgacacccga accacgaggc
    22141 gaaaaatgat taacgtacaa aacgtcagta aaaccttcat cctgcaccag caaaacggcg
    22201 tgcgcctgcc cgtcctcaac cgcgcctcgc tcaccgtcaa cgcgggcgaa tgcgtggtgc
    22261 tccacggcca ctccggcagc ggcaaatcaa ccctgctgcg ctcgctgtac gccaactatc
    22321 tgcccgatga agggcaaatc cagatcaaac acggtgacga gtgggtagac ctggtcaccg
    22381 cgccagcgcg caaagtggtg gagatccgca aaaccaccgt cggctgggtg agccagtttc
    22441 tgcgcgtcat cccgcgtatt tcagcactgg aagtggtgat gcagccgctg ctcgataccg
    22501 gcgtaccgcg tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc ctgaacgtgc
    22561 cggaacgcct gtggcacctg gcgccatcaa ccttctccgg cggcgaacag cagcgcgtca
    22621 acatcgcccg cggctttatc gtcgactacc cgattctgct gctcgacgaa cctaccgcct
    22681 cgctggacgc caaaaacagc gccgcggtgg tggaactgat ccgcgaagcc aaagctcgtg
    22741 gcgcagccat tgtcggcatc ttccatgacg aagccgtacg taatgacgtt gccgaccgcc
    22801 tgcacccaat ggaagcctct tcatgattat caataacgtc aaattggtgc tggaaaacga
    22861 agtggtacac ggttcactgg aggtgcagga cggcgaaatc cgcgcctttg ccgaaagcca
    22921 gagccgcctg ccggaggcga tggacggcga aggcggctgg ctgctgccgg ggctgattga
    22981 gctgcatacc gataatctgg ataaattctt caccccgcgc ccgaaagtcg actggcccgc
    23041 ccattcggcg atgagcagcc acgacgcgct gatggtggca agcggcatca ccaccgtact
    23101 ggacgccgtg gcgattggtg atgtgcgcga cggcggcgat cggttggaga atctggagaa
    23161 gatgatcaac gccatcgaag agacgcagaa acgcggcgtc aaccgcgccg agcaccgtct
    23221 gctcctgcgc tgcgaactgc cgcatcacac cacgctgccg ctgtttgaaa aactggtgca
    23281 gcgcgagccg gtgacgctgg tgtcgctgat ggaccactcg ccgggccagc gccagttcgc
    23341 caaccgcgag aagtatcgcg aatattatca gggcaaatac tcgctcaccg atgcgcagat
    23401 gcagcagtac gaagaagagc aactggcgct tgccgcacgc tggtcgcagc cgaatcgcga
    23461 atccatcgcc gccctgtgcc gcgcgcgaca aattgcgctc gctagccatg atgacgccac
    23521 ccacgcccac gttgccgaat cccacctgct tggcagcgtg atcgccgaat ttcccaccac
    23581 gtttgaagcg gcggaagcct cgcgcaagca tggcatgaac gtactgatgg gcgcgccgaa
    23641 tatcgtgcgc agcggttcgc actccggcaa cgtggcggcc agtgaactgg cgcagcttgg
    23701 cctgctggat atcctctctt ccgactacta ccccaccagc ctgctggatg cggcatttcg
    23761 cgtcgccgat gaccagagca accgctttac gctgccgcag gcggtgaagt tagtgaccca
    23821 aaatccggcg caggcgctta atcttcagga tcgcggggtg attggcgagg gcaaacgcgc
    23881 ggacctggtg ctggcacatc gcaaggacaa tcatattcat atcgaccacg tctggcgtca
    23941 gggtaaaagg gtgttctgat gatgggaaaa ctgatttggt taatggggcc gtccggctcc
    24001 gggaaagaca gcctgctggc ggaaatccgc ctgcgggaac aaactcagtt actggtggcg
    24061 catcgctaca ttacgcgcga cgccagcgcc ggaagtgaaa accatatcgc cctgagcgag
    24121 caggagtttt ttacccgcgc ggggcaaaat ctgttggcct taagctggca cgctaacagt
    24181 ctgtattatg gcgtcggcgt tgagattgat ctctggctgc acgccggatt cgacgtgctg
    24241 gtcaacggct cacgcgccca tctgccgcag gcgctggcgc gctatcaatc ggcgctgctg
    24301 cccatttgct tactggtttc gccggagatc ctgcgccagc gcctcgaaaa ccgtggtcgt
    24361 gaaaatgcca gtgaaattaa cgcccgacta gcacgcgctg cccgctatac tcctcaggat
    24421 tgtcttacgc tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg
    24481 attcatcaga aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtacga
    24541 caccgacgca gtttacgcgc tgatttgtga gctaaaacag gcggagtttg accaccatgc
    24601 gtttcgcgtg ggctttaacg ccaacctgcg cgacccaaac atgcgctacc atctggcgct
    24661 gctggatggc gaagttgtca gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt
    24721 caactggatc ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg gtctgaacgt
    24781 cggcagtaaa ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac
    24841 cgcgctttcg accaacgtga aacgccacga cgcgcaccgt ttctatctgc gagaaggcta
    24901 cgagcagagt cacttccgct tcaccaaggt gctgtaacat gagcctgacc ctcacgctca
    24961 ccggcactgg cggcgcacag ggcgttccgg catggggctg cgagtgcgcg gcctgcgacc
    25021 gagcgcagcg ctcaccgcag tatcgccgcc agccatgcag cggcgtagtg aagtttaacg
    25081 acgcaatcac cctgatcgac gccgggctgc acgatctcgc cgatcgctgg tcgcccggat
    25141 cgttccagca gtttttgctg acgcattatc atatggatca cgcccagggg ctgtttccgc
    25201 tgcgctgggg cgtgggcgat ccgatcccga tctacggccc gccggatgac cagggttgcg
    25261 acgatctgtt taaacatccg ggcctgcttg atttcagcca cacagtggaa ccgtttgtgg
    25321 tgtttgattt gcaggggttg caggtcacgc cgctgccgct taaccactca aaactgacct
    25381 tcggttatct gctggaaacg gcacatagcc gggtggcgtg gctatctgac accgctggcc
    25441 tgccggaaaa aacgctgaaa tttttacgca ataatcagcc acaggtaatg gtgattgatt
    25501 gcagccaccc gccgcgcgcg gatgcgccgc gtaatcactg tgatttaaat accgtgcttg
    25561 cgctgaatca ggttatccgc tcgccacggg tgattctgac ccatatcagc caccagtttg
    25621 atgcgtggct gatagaaaac gcactgccgt cggggtttga ggcggggttt gatgggatgg
    25681 agattggggt ggcgtgatga gagggaatgt gcgcgctggt cccctcaccc taaccctctc
    25741 cccagagggg cgaggggagt gattgtgctc gatattgaat attgcgctgg ttttctccct
    25801 ctccccattg gggtgagggg cgatgcctgc tccataccca acctcatcgc ccatactcat
    25861 cttccattct ccgctcttca tcttctagct gccgacgctc ctgatcaagc tggcgctggc
    25921 gatcgtccag ctgcctgcgg cgatcttcaa actggcagcg gcggtcgtca tattgtctgc
    25981 gccgatcgtc gctcacttca cgctgccagc cgtcgtcgcg cgaatcttca tagtctcgcc
    26041 cacggtcaga gttataagcg tcattaatcg cctgctgaat attaccaata gtatcgtcga
    26101 taatatcggc ctgggccgga acggtggaca gcgtgagcag ggtgaaaaga aaaagcgtgg
    26161 agtaaggttt cataagccag tctctgcgat ggactggctt aacgttagtt gagtgtgaat
    26221 gaccggagta gcggagtaat ctcaaattcg gtaaggcgca gactcttcca ttagctggtt
    26281 agctcgctgt ttattttccg ccaccaatac aatgccatga catttttgat ccggtgaaat
    26341 actccagata cgctcttccg gaatgcagca gccatcgtag atcactttcg tgccatcgta
    26401 gggccaaaat gccccttgtt cagtgtctaa tacaaacacg gaaatctgat cggactcgcg
    26461 cgcgatcttc tgccagagca tcttcccgcc atcatattga tcaaaatcgc tgattagcgt
    26521 gataccgcat tgcaggatta gcgcttcata cagccgagtt gccagaccct tatcgcgctc
    26581 acctggagag acacaaatac cttccacctg taccagatcc gagtcttccc accagcgttc
    26641 aacggcaatg atatcgctgc cgaactcttt tttacgctgc agatcgagag cggcaagtac
    26701 ccgatgacgc ggcacgcctg caaaaacttc aatgtccgct tcaatgatgg ccgctcttgg
    26761 cgtctggttg tcataaatta cctgtaacag acgtccagaa agctcgatct cttccaccac
    26821 gcgaaaagaa cgagcaaccg tcatagggct ataggtggtt ggcatcacga tcatatgtaa
    26881 atcacttcaa ttcagaacag aattgctcac aatagcagca cgtttgcttc gagcctcttg
    26941 ataaattctg aaaatcatcc ctcaatttgc cccagcacct gcgccagctg cgcgcgggta
    27001 aagggtttgc gcagcagcgc cacgtccggc agcgcggggt tatggctggg gcgtaaatcc
    27061 tgaccgctga tgagtagcag cgtcaggtgc gggtaaagtt tgcgggccgc attgaccact
    27121 tctgcgccgc tcatgccgcc agcaggtcca gcgcctgttc gccgctgctc gcttccagtg
    27181 tcaaatagcc cagcaggtgc aactgctcgc agatggtctg gcgcaccgcg gcttcatctt
    27241 ccagcactaa caccagttta tcgccattaa cagctgtctg ttctaccgcc gctggcgata
    27301 ggtttttcgc cgccgtcagc gcgcggggaa gctgtaatct gaccgttgtc ccctgccccg
    27361 gcgcgctttc aatttccacg cgcccgccgg actggcgcac gaaaccatag accatcgaca
    27421 agcccagccc gctgccgctc cccgtctgtt tggtggtaaa gaacggctcg aacacctggc
    27481 ttttcaccgc ctgcgacata ccgctgccat gatcgatgac ctccagtgcc accatgtcct
    27541 gacgctgccc actgctgcgg gtaacacgct gattccaggt acgaattttg ataattcccg
    27601 tctgcccttc catcgcgtcg cgggcgttca tcaccaggtt gatgatggcg ttttccagtt
    27661 ggctgacgtc aatccacgcg gaccacgccg gagactgcgc ttcaatctca atcgtcagcg
    27721 tcgccggtaa ggagtggcgc attaattcgc ttaactcttc cagcagtgtt tttagttcaa
    27781 cggcttgcgg atgtaaggac tgcttgcggg aaaatgccaa caatcgctgg gtcagtagcg
    27841 cgccacgttc ggcggcttgc tgcgcccgct gtattcgcgg cgcgtcgggt gagttcgtgt
    27901 tcaccagttc caggctacca ataattaccg ccagcagatt attaaaatca tgggccagcc
    27961 cgcccgtcag ttgcctgacc gcttttaact tctggctgtg caatagcgcc tcttccagcc
    28021 cctgccgctc ggtttgggca atctccatct gcgtggtttt ctctttcaac agccgtgacg
    28081 ttcgttcaag tgatgcggta ttgcgggcaa aaacgttaaa ggcgcgcgcc agttcaccca
    28141 actcatcgcg ccgctgtaac gccggaaaag tgacgtccgc ttcgccgtgc gccaggcggc
    28201 tcatcgcgcg ggaaatcgcc gtcaggttag agccgagatt gcgatagata taccaacctg
    28261 caaacgcggt gatcaccacc gctaacaggg cgaaaatcat gatcagcctc tcgccggagc
    28321 gcaaatcctg gtaactttgc tccacccgct gaccagagag cgtcgccacc tgttcggcgt
    28381 aacggttgat gtcgccatta aggatcgcca ccagcgcttt gatgtgaaac atataccagc
    28441 tgattgccag atcgctttgc tccagagccg ccgaaagtgg cgcgagttta cgcagttcac
    28501 gattaaaatc agcgagtagc gtattaacca atggcgtatc gctgtgagtt ggcaaggcag
    28561 acatcactcc gaccagttga tgaataatcg cctgcggcgc gggggtggca atggcggcaa
    28621 caatcagtcg gttcatctct ttaagcaaaa tattatcttg cgcgtgagtg actttttgca
    28681 gatgccgcag ataacttaag ttttgataca gcgaacttaa cagcgcgtta cgctccagat
    28741 gccgcctttg cccgcgttcc agcattccgc cgacgctggt ttgcagttcg ttgctacgcg
    28801 tgatgatgcg cgtcaccagc ccggcttcct gctgcgctaa aggcgcgttc gccagttgtg
    28861 caagcgagct ttttaatgcc cgctgtgtct cctgcaaccg ctcggcttcg cctttatatt
    28921 ccagtgctcc caccacctgc gacaatcgca ctgccgccgt tgccacgttt gccgtgtcgc
    28981 gcgccagatt caggctgccg gtcatatcat ctacagtttg ctgcttcacc agctcttgca
    29041 tctggctggc atgacgaaag cccattaccg ccacgccgct gaccatcaac gtcaccgcca
    29101 ccaccagcaa attcaaaagc agcaatcgcc cgcgcgcgct ggcaaaaaag tgacgtccgg
    29161 ggtgcgccat atcacgctcc gtgaagaaaa atcgcaatat gacaaatatg aacggcttct
    29221 gacattttgc atttactttc ctgtgatgga gtgctgatat cgacaacctc gcaggagtcc
    29281 accatgaaca gcacgccggt tcttgagatg cgcaatattg ccaaagcctt cggcaaattt
    29341 tatgcgctca aaggggtgga tttgacggtc taccctggcg agatccatgc cctgatgggg
    29401 gaaaacggcg cgggaaaaag cacgctgatg aaggtgctgg cgggggcgta taccgccacc
    29461 agcggcgaga tcctgataga cggcaagccc tttcacattc gcacgccaaa agatgcctta
    29521 agcgccggta ttacgctgat ttatcaggag atgcagctgg cacccaattt gacggtggca
    29581 gaaaatattt tcctcggcag cgagctttcc cacggcgggc tggtgcagcg aaaagagatg
    29641 ctggtgcagg cgcaaagagt gatcgaccgc ctcggcgcgc agtttaacgc cagcgataag
    29701 gtcatgacgc tgaccattgc cgaacaacag caggtcgaaa tcgcccgcgc gctacatcgc
    29761 aacagccgca ttctggtgat ggacgaaccc accgctgccc tctcctcccg tgaaactcac
    29821 cgtctttttg aactgattat gcggctgcgc gatgaaggga tggcgattat ctacatcagc
    29881 caccgtatgg cggaagtgta tgagctttcc gatcgcgtca gcgtgctgcg cgacgggcaa
    29941 tacgttggca gcctgacccg cgataacctc aatgccgggg agctggtgcg gatgatggtc
    30001 ggcaggccac tgagcgatct gttcaataaa gagcgcgata tcccgctcgg taaagcccgc
    30061 ctgaatgttc accacctgac cgacggcggc aaagtccagc cgagctgcct gcaggtgctt
    30121 tccggcgaaa ttgttggcct cgccgggctg gtgggagccg gacgttccga actggcgcag
    30181 ttaattttcg gcgtgcggaa agcgacaggc ggaatgattg aagtcgatgg cgaaccggtg
    30241 gtgatccact ccccgcgcga agccatcgat cttggcattg gttttctcac cgaaaaccgc
    30301 aaagaacaag gcttattcct tgaaatggcg gcagtcgaaa acatcactat ggcaaccctg
    30361 gagcgcgatg ccagctgggg aatgctcaat cgcaaaaaag cgcaaaccat ttccgatgac
    30421 gccattaagt tgctcaacat tcgcgtgcct catgcccagg tacgcgcggg cgggctttcc
    30481 ggcggcaatc agcaaaaact gttgatctcc cgctgggtgg cgattggccc gcgcatttta
    30541 ctgctcgatg aacccacccg cggcgtggac gttggcgcaa aaagtgagat ctaccggatc
    30601 atgaacgaga tggcgcgcaa gggcgtggcg atcctgatga tctccagcga actgccggag
    30661 atagtgggaa tgagcgatcg cgtccatgtg atgcgcgaag gcagcattgc cggtgagcta
    30721 aatggcaaaa acatcaccca ggaaaacatt atgaccttag cgactggcgt caacgacgcc
    30781 cattcccagg cggtgacctc atgacgacct ctgcaaaaac ctctgccaaa aaaatgctga
    30841 tgagcgatct gatgcaaacg gtcggcattt tgcccatttt gatcctgatc gtagcggtat
    30901 ttggctttat cgcgccaaac ttttttaccg aaagcaacct gctaaatatt acccgccagg
    30961 cgtcgatcaa cattgtgctg gcggcgggga tgaccttcat cattttgacc ggtgggatcg
    31021 atctttctgt aggttcaatc ctcggcacca cggcggtggc ggcaatggtt gtctccctta
    31081 gcccggagtt cgccatgttg tctatcccgg cggcgctgat gctcggcctg ctgctggggc
    31141 tgttcaacgg cgcgctggtg gcctttgccg gattgccgcc gtttatcgtc acgcttggca
    31201 cctatacggc gctgcgcggg gcggcgtatc tgctggctga tggcaccacg gttatcaact
    31261 ccaacattaa tttcgagtgg ataggcaata actatctcgg gccggtcccg tggctggtgg
    31321 tgattgcgct ggcggtgatc gtgctgtgct ggtttattct gcgtcgcacc acgcttggcg
    31381 ttcatatcta cgccgtcggt ggcaatatgc aggcggcgcg tcttaccggc attaaggtct
    31441 ggctggtgct gctgtttgtt tatggcatga gcggcctgct ttcagggctt ggcgggatta
    31501 tgagcacctc gcggctgtac agcgccaacg gcaacttagg tatgggttac gaactggatg
    31561 ccattgcggc ggtgatcctc ggcggcacca gttttgtcgg cggcatcggc accatcaccg
    31621 gcacgctggt gggggcgcta attatcgcca cgctcaacaa tggcatgacc ttaatgggcg
    31681 tctcttactt ctggcagctg gtgatcaaag gggcggtgat catcattgcg gtgcttatcg
    31741 acaaataccg tacccgacat catcaaagtg cataacaaca ataccctaca catgaggaat
    31801 acagcatgag attgaagccc ttagtgaccg cgctctgtgc aggcgcgctg ctggccgcag
    31861 caccgttcgc ccaggcgaaa gatctgaaat cgataggcgt aacggtaggt gacctggcga
    31921 acccgttctt cgtacagatc accaaaggcg cggagctgga agcacgcaaa ctggcgggcg
    31981 ataacgtcaa agtgacgctg gtttccagcg gctacgatct gggtcagcag gtctcgcaga
    32041 tagacaactt tatcgccgct aacgtcgata tgatcatcct taacgctgcc gattcaaaag
    32101 ggatcggccc ggcggtgaaa cgggcaaaag acgccgggat cgtggtggtg gcggttgacg
    32161 tggcggcaga aggtgccgat gccaccatca cttccgataa cacccaagca ggcgaaatgg
    32221 cctgtaaata catcaccgat cgcctgaaag gcaaaggcaa cgtggtgatc atcaacggac
    32281 cgccggtgtc cgccgtgcaa aaccgcgtcg aaggctgcca gaccgaattt aaacgccatc
    32341 ctgacatcaa ggtgctctct gacaacaaga acgccaaagg cagtcgtgag ggcgggctgg
    32401 aggtgatgac cgggctgctg gcggcaaatc cgaagatcga cggcgtattt gcgattaacg
    32461 atccgacagc gatcggtgcc gatctggcag caaaacaggc gcaacgtaac gagttcttta
    32521 ttgtcggcgt ggatggaagc ccggacgccg aagaggcgct caagcgtgag aactcgttgt
    32581 ttgtcgctac tccggcgcaa gatccacagg tgatggcagc gaaagcggtt gagatcagtt
    32641 atgacatttt gcagggcaaa cctgcgccga aagagccggt gctgatcccg gtgacgatga
    32701 tcgataaaaa caacgtcggc aattacaaag gctggacggt gaaataagtc cacacgccgg
    32761 gcggcgatgc tgcccggcca ccgctaagga gaacgctttc gatgaaacgc tccgctataa
    32821 acaacattct cggccataca cggcaattct tttcgcaaca tgacgtacat ctgccgccct
    32881 ttgccagttt ctctcctgct caatggcagc agctcgacac cgccgcgtgg gaggtggttt
    32941 ttgatctcaa actcggctgg gatgttaccg cttttggtgg taacaacttt gcagcacaga
    33001 ggctgacgct gtttaccctg cgtaacggct cagcaaaagg aatgccgtat gtgaaatgtt
    33061 acgccgagaa gatcatgcat gtgcgcgatg cgcaggtcac gccaatgcat tttcactggc
    33121 gtaagcgtga ggacatcatc aatcgcggcg gcggcaattt gattgttgaa ctgtggaatg
    33181 ccgatagcaa cgagcaaaca gttgataccg acgttacggt ggtgatcgac ggctgccgcc
    33241 agactcatgt cgcaggcagt cagttacgcc tgtcccccgg cgaaagtatc tgcctgccgc
    33301 ccggcctgta tcacagcttt tgggcggaaa ccggttttgg cgatgttctg gttggcgaag
    33361 tctcttctgt caatgacgac gaccacgata accacttttt acaaccgctg gaccgctaca
    33421 acctgattga cgaagacgaa ccggcccagc tggtgttgtg taacgaatac cgccagttcc
    33481 tctgaataag gataacgtta tgccgttgat ttctctcgct gaagggctgg cacatgcccg
    33541 cgaacatcac tatgcgttag gtgcgtttaa tgtgctggac tcacactttt tacgcgcctt
    33601 gtttgctgct gccagacaag aacgttcgcc ctttattatc aacattgccg aagtacattt
    33661 taaatatatc tcgctggatt ccctggtcga gacagtgaaa tttgaagcgg cgcgtcaagc
    33721 gattccggtg gtattaaatc tcgatcacgg gctgcatttt gaatcggtgg cgcaagcgtt
    33781 gcggctggga ttcagctcgg taatgtttga tggctccacg ctggggtatg aagagaatat
    33841 ccgccagacg cgggaagtgg tgaaaatgtg ccatgccgtt ggcgtatcgg tagaagctga
    33901 actgggcgcg gtgggtggtg acgaaggcgg tgcgctttac ggtcatgccg atgaatctct
    33961 gtttaccgat ccgcagctgg cgcgtgattt tgtcgaccgt accggcattg acgcgctggc
    34021 cgtggcgatt ggcaatgcgc acggcaaata tcgcggcgaa ccgaaactcg atttcccgcg
    34081 cctcgacgct atccgccagc aggcggctat cccgctggta ttacacggcg gttcagggat
    34141 tagagatgcc gatttccgcc gcgccatcga actgggtatc cacaaaatca atttctacac
    34201 cggcatgtca caagcggcgc tggctgccgt tgaggcccga atggcgcacc gtcagccgat
    34261 gtatgacgag tttgccgagt tactactagc agtggaagcc gccatcagcg atacggttgc
    34321 acaacagatg cgtattttcg gcagtgcggg gcaggcatga tggaacgtaa agggattatc
    34381 gccgcaggca atatgctggt tgaccacgtc caccagatta tccagtggcc agagcgcggc
    34441 tggctggcgg agattaccca cagcgaacgc tccaccggcg gcgcgccgct taacgtcctg
    34501 ctgacactgg cgaaaatgcg caccgggttg ctgttacagg cggtcgggct aatcggtcag
    34561 gacagcgacg gcgattacat tatggcgatg cttgagcaat atcacgtcaa tcgtcagcat
    34621 gtgcagcgca ctacctttgc cccgacgtca atgtcgcagg taatgactga tccaagcggg
    34681 cagcggacgt ttttccattc gccaggtgca aacagacttt tggatcttcc ggcgttcgat
    34741 cagctcgata acaccatgaa gattttccat ctcggctatt tactgttgct cgacagcctg
    34801 gatatgccgg acgatgttta cggcacccgt agcgcacgcc tgctggcgca aatgcgtgat
    34861 actggctttg aaacctcact cgacctggtc tcccgcaagg gcgatccgcg ttatcagccg
    34921 ctggtgttgc ccgcgttgcg ccatctggat tacctggtaa ttaacgaact ggaggccggg
    34981 gagtttagcg ggctggaaat tcggttaccc aacggcgaac cgcatatcgc gcatattgct
    35041 gccgccgcgc gtgaactgtt agatgcgggc gtgcgccagc gtgtggtgat ccactgcccg
    35101 gaaggggcgt ggggagagac cccggaacag tgcggcgtgt ggatcccttc gcaaaagtta
    35161 gaacaacggg aaattatcgg cagcgtcggt gcgggcgatg tattttgtgc cggattttta
    35221 tatggctgcc atgaacgctg gacgcttacc gagagcatca aactggcaca cgcctgcgcg
    35281 cgagcaagcc tgttatgcgc caatgccatc gacggggcga aaacgctgga agagctgcaa
    35341 acagaaatgt cggattaatg cacgtcggca gcgaagacat accccagacc gcgcagagtt
    35401 ttaatcaaca tcggctgatg cgggttgagt tcgattttgc gtcgtaagcg catgattaat
    35461 acgtcgatgg tgcgatcaaa cacctccacg ctgtcgctat gagtgaacgc cagcagctgt
    35521 tcgcggctta atacccgacg ggcattttga gccagggcca gtaacaagcc atattcgccc
    35581 tgagtcagct cgacatgttg ttgtagcgga ctatgcagtt ggcagcgaat ggtatcgaga
    35641 taccagccat tgaatgacag cccggcgttt ttctcctcat taaccgccag cgcgccaacc
    35701 cgtcgcaaca cgctttcagt cgtgcgacca ccacccgtgg ggtgaacggc ttgcagatgt
    35761 aatcatccgc gcccatttcc agcccgacca ccacatcaga ctcactcccc attccggtca
    35821 acatcaccac aggcaatgcc ggacgcagtt tctggatttg ctgcaatacc ctcaggccgt
    35881 tggtatcggg cagcatcata tccagcacca ccagcgcaat gtcaggctcg ccctcaatac
    35941 gcgatatggc ctcctgtccg gtatggctga ctgaaaccgt aaagacatgt tcacttaaca
    36001 cgtcctgcaa cagtgcgcag atagccgtgt catcatccac caccagcacg acgggtttca
    36061 tggttacact ccgcatcatt catctaatta atcattgtgc gtacttcttg tcgaagtgaa
    36121 agcggaaagt aatataaata ataaaaatat aagtcaggtc acattattga tgatagtaat
    36181 aaatatgccg tgcttttccc tcttcagcac aaatgcctaa taaaatggcg cgtcccgctc
    36241 acacttccac ttattaaagt cgcaaattcc caattatgat aaccccagct ttaaatgacg
    36301 tattttgcat ggattacata tttacgacac aaatgataaa tggagattta tatgaataac
    36361 tctcggttat tccgtttgag cagaattgtt attgcgttaa ctgccgccag cggcatgatg
    36421 gtaaataccg ctaacgcgaa agaggaagcg aaagccgcca ctcaatatac ccaacaggtt
    36481 aatcagaatt acgccaaatc attaccgttt agcgatcgtc aggattttga cgacgctcag
    36541 cgtggattta tcgctccgct gctggatgaa ggtattctgc gcgatgctaa tggcaaacca
    36601 tattatc
//
LOCUS       DAJTNM010000023        35477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTNM010000023 DAJTNM010000000
VERSION     DAJTNM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.023"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(66..1541)
                     /locus_tag="OO172_001310"
     CDS             complement(66..1541)
                     /locus_tag="OO172_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5511558.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(1688..2972)
                     /locus_tag="OO172_001311"
                     /pseudo
     CDS             complement(1688..2972)
                     /locus_tag="OO172_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3085..5019)
                     /gene="yeaG"
                     /locus_tag="OO172_001312"
     CDS             complement(3085..5019)
                     /gene="yeaG"
                     /locus_tag="OO172_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5511559.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGTLCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILPRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5455..6201
                     /gene="mipA"
                     /locus_tag="OO172_001313"
     CDS             5455..6201
                     /gene="mipA"
                     /locus_tag="OO172_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5511560.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6291..7052
                     /locus_tag="OO172_001314"
     CDS             6291..7052
                     /locus_tag="OO172_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5511561.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7103..7987)
                     /locus_tag="OO172_001315"
     CDS             complement(7103..7987)
                     /locus_tag="OO172_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5511562.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8071..9066)
                     /gene="gapA"
                     /locus_tag="OO172_001316"
     CDS             complement(8071..9066)
                     /gene="gapA"
                     /locus_tag="OO172_001316"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5511563.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9408..9821
                     /gene="msrB"
                     /locus_tag="OO172_001317"
     CDS             9408..9821
                     /gene="msrB"
                     /locus_tag="OO172_001317"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5511564.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            9863..10135
                     /locus_tag="OO172_001318"
     CDS             9863..10135
                     /locus_tag="OO172_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5511565.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10505..11581
                     /locus_tag="OO172_001319"
     CDS             10505..11581
                     /locus_tag="OO172_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5511566.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11608..12987
                     /locus_tag="OO172_001320"
     CDS             11608..12987
                     /locus_tag="OO172_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5511567.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     SLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            13004..14049
                     /locus_tag="OO172_001321"
                     /pseudo
     CDS             13004..14049
                     /locus_tag="OO172_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14070..14906
                     /locus_tag="OO172_001322"
     CDS             14070..14906
                     /locus_tag="OO172_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5511568.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            14911..15859
                     /locus_tag="OO172_001323"
                     /pseudo
     CDS             14911..15859
                     /locus_tag="OO172_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            15869..16849
                     /gene="ydjG"
                     /locus_tag="OO172_001324"
     CDS             15869..16849
                     /gene="ydjG"
                     /locus_tag="OO172_001324"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5511569.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            16986..17744
                     /locus_tag="OO172_001325"
     CDS             16986..17744
                     /locus_tag="OO172_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5511570.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            17862..19220
                     /locus_tag="OO172_001326"
     CDS             17862..19220
                     /locus_tag="OO172_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5511571.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19313..19954)
                     /gene="pncA"
                     /locus_tag="OO172_001327"
     CDS             complement(19313..19954)
                     /gene="pncA"
                     /locus_tag="OO172_001327"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5511572.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(19965..20981)
                     /gene="ansA"
                     /locus_tag="OO172_001328"
     CDS             complement(19965..20981)
                     /gene="ansA"
                     /locus_tag="OO172_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5511573.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TLDD"
     gene            complement(21148..23004)
                     /gene="sppA"
                     /locus_tag="OO172_001329"
     CDS             complement(21148..23004)
                     /gene="sppA"
                     /locus_tag="OO172_001329"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5511574.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23165..23716
                     /locus_tag="OO172_001330"
     CDS             23165..23716
                     /locus_tag="OO172_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5511575.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            23833..24876
                     /gene="selD"
                     /locus_tag="OO172_001331"
     CDS             23833..24876
                     /gene="selD"
                     /locus_tag="OO172_001331"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5511576.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMQREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            24881..26842
                     /gene="topB"
                     /locus_tag="OO172_001332"
     CDS             24881..26842
                     /gene="topB"
                     /locus_tag="OO172_001332"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5511577.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            26969..28009
                     /locus_tag="OO172_001333"
     CDS             26969..28009
                     /locus_tag="OO172_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5511578.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQLGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28126..29469)
                     /gene="gdhA"
                     /locus_tag="OO172_001334"
     CDS             complement(28126..29469)
                     /gene="gdhA"
                     /locus_tag="OO172_001334"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5511579.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            29705..29977
                     /locus_tag="OO172_001335"
     CDS             29705..29977
                     /locus_tag="OO172_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5511580.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(29943..30350)
                     /gene="nudG"
                     /locus_tag="OO172_001336"
     CDS             complement(29943..30350)
                     /gene="nudG"
                     /locus_tag="OO172_001336"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5511581.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30437..31084
                     /locus_tag="OO172_001337"
     CDS             30437..31084
                     /locus_tag="OO172_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5511582.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(31093..32400)
                     /gene="ynjE"
                     /locus_tag="OO172_001338"
     CDS             complement(31093..32400)
                     /gene="ynjE"
                     /locus_tag="OO172_001338"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR5511583.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGGWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(32467..33120)
                     /locus_tag="OO172_001339"
     CDS             complement(32467..33120)
                     /locus_tag="OO172_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5511584.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33120..34654)
                     /locus_tag="OO172_001340"
                     /pseudo
     CDS             complement(33120..34654)
                     /locus_tag="OO172_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34627..>35477)
                     /locus_tag="OO172_001341"
     CDS             complement(34627..>35477)
                     /locus_tag="OO172_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5511585.1"
                     /translation="LKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGG
                     AQLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDP
                     AALKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLT
                     FNPAHAQQKIASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPD
                     AQLRKADPVVWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWL
                     RRYGTH"
BASE COUNT         8985 a         8405 c         9079 g         9008 t
ORIGIN      
        1 ggggtaatag taataaattg tatgcaaaag ccacgcaaaa agcatggctc tgaatatttt
       61 tgcacttaaa tattatgaat aatcactttg ttgcccccct cgcttttcgc ttcgcgtaag
      121 gctttatctg cttccgtcat gacaagcgag atattacgcg tgtcacccgt tgaaaaaaac
      181 gtgccaatac tgatggtcat ttgttcagga acatcatatc tgttactgaa gccggtcagg
      241 agttcgacat ttttacgcat acgttccgca atgatttttg cctgggcact attgagttcg
      301 gtaaatagca agccaaacac ctcaccctcc agtcgcgcta aaatatcatc ggggcggatg
      361 ctttgctgga taatatcgac aattgaaacc agcactttat cacccacacg atgtccccat
      421 tgggcattga tgcgtttgaa ctgattaata tcaaccagca tgacacagaa gtccttaaca
      481 acaggtcgcg acaataatga ctctacgctg ttgaaaaagt agcgccgatt ataaatattg
      541 gtcagcacat catgaactgc cagtttgctg gagagttgca gttcctgaaa aatgttataa
      601 atcagaaaag acacaacaaa cagtttactg gagacttcaa tcccgcgact gatgtaccag
      661 atggtgtaat tatactcatc cagagtcagt agaagtaagt tgcagcatag cgatgccaga
      721 cataataatg ctattaatgg ccatagttcg ctggctaatc gtgtacgcat gataataaag
      781 aacagtaaaa atgcccacag acaaaccaat atttttgtat agttgattcc ccaggtcgca
      841 gtatggttgt caggacagta atcgacgaca tacaaagaat agtcagcgtt ataactgctc
      901 agattgtgtg ccagaagggg aaaaactaaa aaaggaatca gcgccagcaa cagaatgcca
      961 gtttttttct tattgttgtc aaggatgttg tctttaccat aacaaaagag cgccaacgag
     1021 gttaaacaaa taaaactgag ttggcggaaa atatagaaaa tagaaacatc attcgacttt
     1081 gtctggatca gtgtactgtc gctgataggt ttatggataa caacaatagt ttcaactaaa
     1141 tagataaggc cacttaaaag tgcaagactt aaaatgagtg ttggtagaat ttgtttgtta
     1201 gtgaaatatt tcattaacat gaaaagaaaa atgattgcgt taaggaaaaa caaagccaca
     1261 acgaccatca gataattcat ttgtgggatg agttttacat tcgtcttttc gttatataat
     1321 gtggtgatgc aaaataaaat taacgtcagc aataaactga aaataaaaaa gctttgagct
     1381 tttaattttt gtgtgcccat agaaaccata aaaaggagag caatgtgagt gctggatttt
     1441 attgatggca gagatatata gttttgacca agatcaattt tcgttaacga taaaaaatat
     1501 ttaaagaata accccaggga aatacgtgtt gactgaatca tttttccgaa tgattgtttt
     1561 ctggaactaa attgtgtaaa ggttatgctg tcatatttaa agcgattgta agctaatgta
     1621 tgtaataaat gagataattt acagtaagct aaaaatcagc caggcgataa tgacccggct
     1681 gatagtttta gtctttagct gttgcatttt gtttatgaaa cagttcacgg aacaccggat
     1741 aaatatcatc ctggtcgcgg atgtgctgca tcgcaaagtt gtcgaaagta gattgcagat
     1801 gctcatattc tcgcacacaa tgtctgatgt gcacgacggg taatttcgat atagctgtaa
     1861 taacgaacaa caggtaataa ttttttcgcc aggatttcat ggcaaagcgg agagtcatcg
     1921 gcccagttat cgccgtccga tgcttgtgca gcgtaaatat tccactgtgc cgggttataa
     1981 cgctctttca ctacctcatc catcagtttc agggcgctgg aaacaatggt gccgcctgtt
     2041 tcctgcgagt agaaaaactc atgttcatcg acttctttcg cctgggtatg atggcggatg
     2101 tatacgactt ccacgttctt atacgttctg ctgaggaaca gatacagcag aatataaaaa
     2161 cgcttagcca tatctttagt ggattgatcc attgaaccgg aaacgtccat caggcaaaac
     2221 atcactgcct ggctggaggg atcgggccgc ttctcgtagt tcttgtaacg taaatcgaag
     2281 gtgtcaataa aagggacgcg ttcaattttg gcacgtaatt ctgcaatttc tttgcgcaga
     2341 cgttcctctt ccagcagctg cgcaggttca ctgttgctga tgatggccaa attctcttcc
     2401 agtgcatgaa gttcccgccg cttgcctgcc gtcatggctg tgcgtcgcgc cagtgagttc
     2461 tgcaaggaac gcacaacgct gatattggct ggaacgccgt tagcggtata acccgcccga
     2521 tgcgttttat attcggtcag ctggcgttgt tggttttgtt tcagattcgg taaggccaaa
     2581 tcttcaaaga gcagatcaag atactcatct ttcgaaatct gaaagacaaa ttcatcctga
     2641 ccttcaccat cctggctggc ctggccctga ccactgccgg aacctccgcc gccaccctgg
     2701 ggacgttcaa ttcggtcgtt ctgaacgaaa tggtcattgc ccggatgcac gcggtggcgc
     2761 agaccgccac gcccctgatg aaacattggt tcgctaatat cttccgtggg aatggatacg
     2821 gattcgctgc tgtcgacgtc agtcaccgaa cgcttattaa tggcctcgga gatcgactgt
     2881 ttaatttgcg ctttataacg gcgtaaaaaa cgctggcgat tcaccatgct tttgtttttg
     2941 ccgttcagac gccggtcaat aaaccaggtc atatgccccc cgtactacat ttgccaactg
     3001 tatgctacgc ctgtgttgcc gcttctcagg cccacggttc gctatcgttg taggcccggt
     3061 agcatgtgta ccgggccagg gttgttaaga cgatttacgt acgcgcaaat accattcgca
     3121 cagtaaacgc acctgtttac gggtgtagcc tttttccatc atacggtcga caaagtcgtc
     3181 gtgtttcttc tgctcgtcgg ttgacgtttt ggcgttaaac gagataaccg gcaacagctc
     3241 ctcggtattg gagaacattt tcttctcgat aaccgtgcgc agtttttcat agctggtcca
     3301 gttcggattg cgtccgctgt tattcgctct ggcgcgcagt acgaagttaa caatctcgtt
     3361 gcggaaatct tttggattac tgatccccgc cggtttctcg attttctcca gctcggcgtt
     3421 aagagactcg cggtcaaaca gctgcccggt atccggatcg cgatactcct gatcctgaat
     3481 ccagaaatcc gcgtaggtaa cataacggtc gaaaatgttt tgcccatatt cggaatagga
     3541 ttcaaggtag gccgtctgga tctctttgcc gataaactcg gcatatttcg ggatcagata
     3601 acctttcagg aactccagat agcgttctgc ctgctcttgc gggaactgct cgcgctcgat
     3661 ctgctgttcc aggacgtaga acagatggac cgggtttgcc gccacttcta catgatcgaa
     3721 gttgaacacg cgggggagga tcttaaacgc aaaacgcgtc gacagaccgt tcatcccttc
     3781 atcgacaccg gcgtagtcac gatattcctg atacgacttg gctttcggat cggtgtcttt
     3841 cagactttcg ccatcataaa cccgcatctt tgaataaatg ctggagtttt ctggctcttt
     3901 caggcgcgaa agaatggaaa aacgtgacag tgtttcgagc gtgccagggg cgcatggggc
     3961 gtgagtcaat tcactgtgat taagcaattt ctcgtagatt ttgatctctt cggaaatgcg
     4021 caagcaatac ggcaccttca cgatgtaaac acggtcgagg aaggcttcgt tgtttttgtt
     4081 attacggaaa gtgacccatt cggactcgtt cgagtgggcg agaataatcc cgttgaacgg
     4141 cagggcggag ataccttccg tcccgttgta gttatcttcc tgagtggcgg ttaacaaggg
     4201 atgcagcact tttatcggtg ctttaaacat ctcaacgaat tccatgatcc cctgattggc
     4261 gcggcacagc gtaccggaat agccgtaggc gtccgggtca ttctgcgcgt agtgttcgag
     4321 tttacgaata tcgactttcc caaccagcgc ggagatgtcc tggttgttct catcaccggg
     4381 ttccgttttg gcgatagcaa tttgttgcag aattgacggc cagaccttca ctacccggaa
     4441 cttagtgata tcgccaccaa attcatgcag gcgttttgcc gcccatggcg acatgatggt
     4501 gccgagataa cggcgaggaa tgccatactc tttttccaga atctgagcat cttcctgcgg
     4561 attgaaaaga cagaacggat gatcgttgac cgggctacgc tcaccgttgg cgctcaatac
     4621 ataaatcggt acgagctgca ttaatgattt cagtcgctca gcaagcgatg atttaccccc
     4681 acccacaggc cccagcagat acaggatttg tttcttctct tccagcccct gagccgcgtg
     4741 tttcagataa gagacaatct gttcaatcgc gtcttccatg ccgtaaaact cttcaaatgc
     4801 cggataacgt gcaatgaccc ggttagaaaa gagtcgagaa agtctgggtt cctgggctgt
     4861 atcgaccatg acaggctcac cgatagccat caatagccgc tcagccgcgt tggcataagc
     4921 actgcgatct tgccgacaag tggtaagaaa ctcctgcagt gtgaactctt cgtccttggc
     4981 agcttcatat cgctggcgat agtgatcgaa tatattcatg gtatgccgtc ctttcgtttt
     5041 ttagcacagg ttaagagccg ttcatatgaa tagtagaggc tcccggaaga ggtaaactga
     5101 acgacgtctc ttgctaaaac acagcaactc tcatgccatt ttatcgacga tgagaacaac
     5161 gtgcccggtg atacaccttc aaaattaagc gtagatggca aatggaaaac ttgcctgcac
     5221 ggctttctta atttcaatgg catatcaaca actcatccat aaattttgca taattaatgt
     5281 aaagaccagg ctcgccagta acgctaaatc catttggctg taagcgcggt gtcatccgcg
     5341 gcaggaaaat taaacagtta ctttaaaaaa tgaaaacgta aaaaggttgg gtttcgatgt
     5401 attgacgggt aaactttgtc gcccgctaaa catttgttta aggaatgatt aattgtgacc
     5461 aaactcaaac ttctggcact tggagtgctt atcgcaacgt ctgcaggcgt agcgcacgct
     5521 gaaggtaaat tttccctggg cgcaggcgta ggtgtcgttg agcacccata taaagattac
     5581 gataccgatg tttacccagt accggtaatc aactatgaag gcgataactt ctggttccgt
     5641 ggcttaggtg gtggttacta cctgtggaat gacgcaacgg ataaactttc aattaccgct
     5701 tactggtcgc cgctttactt caaagcgaaa gacagtggcg atcaccaaat gcgtcacctg
     5761 gatgaccgta agagcaccat gatggctggt ctgtcttatg ctcactttac ccagtacggt
     5821 tacctgcgta ccaccctggc tggcgatacc ctggataaca gcaacggcat tgtctgggat
     5881 atggcttggt tgtatcgtta caccaacggt ggcctgaccg tgactccggg tattggtgtg
     5941 cagtggaaca gcgaaaacca gaacgaatac tattatggcg tatcgcgcaa agagtccgct
     6001 cgcagcggtc tgcgtgggta taacccaaat gacagctgga gcccttacct tgagctgagc
     6061 gccagctaca acttcctcgg cgactggagt gtttacggta ccgcgcgcta cacccgtctg
     6121 tctgatgaag ttactgacag cccgatggtg gataaatcct ggactggcct gatttctacc
     6181 gggatcacct acaaattctg ataatgcatc tttccagggc attttgtgca taaaaatagg
     6241 gcgtcacgcc ctgttttgca ttacggtggt gcaatacctg ggaggaaaca atgcaacaaa
     6301 aaatgattca atttagtggc gatgtctcac tgccagccat agggcaggga acatggtata
     6361 tgggcgaaga tgccagtcag cgcaaaacag aagttgctgc actacgcgca ggcattgaac
     6421 tcggtttaac cctcattgat accgccgaaa tgtatgccga tggcggtgcc gaaaaggtgg
     6481 ttggggaagc attaaccggt ctgcgagaga aggtctttct cgtctctaaa gtctatccgt
     6541 ggaatgctgg cgggcaaaaa gcgataaatg catgcgaagc cagtttacgc cgtctcaata
     6601 ctgattatct cgatctctac ttattacact ggtctggcag tttcgctttt gaagagactg
     6661 ttgctgcgat ggaaaaattg atcgcccagg gaaaaatccg tcgctggggc gtttctaacc
     6721 ttggttatgc cgatatgcag gaactctggc agctgccggg gggaaatcag tgtgccacta
     6781 atcaggtgct ttaccatctc ggttcacgag ggattgagta cgatctactc ccctggtgcc
     6841 agcaacagca gatgccggtg atggcttaca gtccattagt ccaggccggg cggttgcgca
     6901 atggactgtt aaaaaacgcg gtagtcaacg aaattgcaca tgtccaacaa aatgcagctg
     6961 cgcttgaggt cgaactttcg tcagcggaat tagctatgct ggataaggca tatccggcac
     7021 caaaaggaaa aactgcgctg gatatggtgt gatgttgata cgtgccggat gcatcgcacc
     7081 cggcacacaa cattaacgca aattaacgtt tggcaacgcg aatggattgc gccagatgtg
     7141 caggtttctc tttggtgact ttttgcgttt ctgaagcgta agccgtttct acacaaacaa
     7201 aggtcttgta gccatcgtcc ggcatgtcgc ccatgctaac tgaaagcgcc ggtcccgggt
     7261 tccagccgac aacgttcaga tgatgctggt ggcctacggc gataatacga ttcagcgctt
     7321 catcattaat cacgctgcaa tcttgcggat tcagatacac gcgatcggta cggtcaggga
     7381 aggtctgaat accgtcggtc agtacatctt ctttcgcgtc attcacttta tcaatgaagc
     7441 gatcgcccag cccactgacg cttaccttag cgatatcacc cacgttaaag taggtatgca
     7501 gggcagaggt ggtttcaaat tcgccatgtg attcaagatc gatttcacag gttttaccca
     7561 cgcggaaatg cgctaacagc gtaaagtcgt gcggccagaa ttttttcgtc tcttcgcttt
     7621 gtgtcaattc aaaagtcagc gctacgccat cagcatcttc atgatgtgat ttcagcgtcc
     7681 acggcaggtt gcgggcaaaa ccgtgcgcag gcagaccttg ttgtgccgcc ggaccaaacc
     7741 acggccagca aaccggtacg ctaccgcgaa tagcgacgcc atttttgaac ggtgtgttgt
     7801 tgctcaacca cagaacttct tcttcacccg caggtttcca cgagagaagg tgtgcgccct
     7861 gtaatgcaaa agaggctttt acctggggat gatcgaccac aatgaggtcc agttcatcca
     7921 gtttacgacg ggagaggaca ggggagattt gttcgatgac cggaagggca aaaattttct
     7981 taatcatgac gcagtccttt aacttcattt tatcaggtaa aaaaaagagc gaccgaagtc
     8041 gctcttttta gatcacagtg tcatctcaac ttatttggag atgtgagcga tcaggtccag
     8101 aactttgttg gagtaaccgg tttcgttgtc gtaccaagat accagtttca cgaagttgtc
     8161 gttcagagcg ataccagctt tagcatcgaa cacggaagtg caaacttcgc cgttgaaatc
     8221 ggtagatact acgtcatctt cggtgtagcc cagaacgcct ttcatttcgc cttcagcagc
     8281 agctttaacg gcagctttga tctgctcgta agttgcagct ttttccagac gaacggtcag
     8341 gtcaactacg gatacgttcg gggtcggaac gcggaacgcc ataccagtca gtttgccatt
     8401 cagttctggc agtactttac ctacagcttt agcagcaccg gtagaggacg ggatgatgtt
     8461 ctgggaagcg ccgcggccgc cgcgccagtc tttgtgagac gggccatcaa cggttttctg
     8521 agtagcggta gtagcgtgaa cggtggtcat cagaccttcg atgatgccga agttatcgtt
     8581 gataacttta gccagcggag ccaggcagtt ggtggtgcag gaagcgttgg aaacgatgtc
     8641 ctggccagca tatttgtcga agttagcgcc tttaacgaac atcggagtgt tgtctttaga
     8701 cggaccagtc ataaccactt tcttcgcacc agcggtgatg tgtttacgag cagtttcgtc
     8761 agtcaggaac agaccagttg cttcagcgac aacgtcaaca cccacttcgt cccatttcag
     8821 gttagccgga tcacgttcag cggtaacacg gattttttta ccgttaacga tcagatgacc
     8881 gtctttcact tcaacggtac cgtcgaaacg gccgtgagtg gagtcatatt tcagcatgta
     8941 tgccatgtaa tcagcgtcta acaggtcgtt gattgcaacg atctcgatgt cagaacgttt
     9001 ctgagcagca cggaaaacaa tgcgaccgat acggccaaaa ccgttgatac ctactttgat
     9061 agtcatatat tccaccagct atttgttagt gaataaaagg ttgcctgtaa aattacaaaa
     9121 accttacgca gcgtcaagcg gaatcgtgtc aatcattgcg acagatcaat cctgtgccta
     9181 agcattacgc gactgactcg cctcactctt cctttgggct tgagaccaca tggggtcggc
     9241 gccccgaatt ttaaagggca attacgataa aaatgtgatt ttcatcacga tttaggtgca
     9301 gccatttcgt cgagatcaag tttaggacaa aattttgcca gttggtgtta atgttttgtt
     9361 agaatcagtc acgtaatgtg agcacgatta aagtgagatg tgagtaaatg gcgaataaac
     9421 cttcggcaga agaactgaaa aaaaatttgt ccgagatgca gttttacgtg acgcagaatc
     9481 atgggacaga accgccattt acgggtcgtt tactgcataa caagcgtgac ggcgtatatc
     9541 actgtttgat ctgcgatgcc ccgctgtttc attccgaaac caagtatgat tccggctgtg
     9601 gctggcccag tttctacgaa ccggtgagtg aagaatccat tcgttatatc aaagacttgt
     9661 cacatggaat gcagcgcata gaaattcgtt gcggtaactg tgatgcccat ctggggcatg
     9721 tctttcccga cgggccgcaa ccaacaggcg aacgttattg tgttaactct gcttctttac
     9781 gcttcactga tggcgaaaac ggcgaagaaa tcaacggttg aaaaaacgat tcagcaactt
     9841 attccacagg agcggaaaaa ttatgaatct tgatgacatt atcaacagca tgacgcctga
     9901 ggtataccag cgtttgtcga ccgctgttga actggggaaa tggcctgatg gcgttgcgtt
     9961 gaccgaggaa caaaaagaga attgcctgca actggtgatg ctgtggcaag cccgccataa
    10021 taccgaagca cagcatatga caattgacac aaatggtcag atggtcatga agagcaaaca
    10081 gcagttaaaa gaagattttg gtatctcagc aaagcctatt gccatgttta agtaagtggt
    10141 tcatcccctt tttgccgcgg ctatgaaggg gatagccaac aatgacccca cgactcgtct
    10201 caattatctc gacccgacct ggctaagtcc cagtccgatc tcttgcagtc gcgttcacgc
    10261 ctgaatgact tctcctgaac ttaagtctca gacctatttg gccggtaatc cctctcgaat
    10321 ttgctctttt tgcgtcccgc cttccaccaa cctgtaacag acccagccat tttctgcgta
    10381 attgtgatct aagttgtaaa atgcaggtca ttatctgtgt tgtgcagatt taatgttgac
    10441 ctcaaaaatc atgtgggtaa gtatgatggc catctaagca aaacaaaaca cagaggatga
    10501 cgcaatgaaa gcactggctc ggtttggcaa ggcctttggc ggctacaaga tgattcatgt
    10561 cccacaaccc atttgtggcc cggaagacgt agtgattgaa attaaagccg cggcaatctg
    10621 cggcgcagac atgaagcact acaatgtcga tagcggttct gatgagttta actctatccg
    10681 cggccatgag ttcgcaggtt gtattgcgca ggttggtgaa aaagtcaaag actggaaagt
    10741 ggggcaacgc gtcgtgtcgg ataacagcgg tcacgtttgc ggcgtttgtc cggcctgtga
    10801 acagggtgat tttctgtgtt gtacagaaaa agtaaacctt ggtctggata acaatacctg
    10861 gggcggtggt ttttccaaat attgtctggt tcctggtgaa attctcaaaa ttcatcgtca
    10921 tgcgttgtgg gaaatccctg atggtgttga ttatgaggac gcagccgtac ttgaccctat
    10981 ctgtaatgcc tataaatcca tcgcgcagca atcgaaattc cttcctggtc aggatgttgt
    11041 cgtcattggc actggcccac tcgggctgtt ttccgtacaa atggcgcgga ttatgggggc
    11101 ggtaaatatc gtcgtcgttg gtctgcaaga agatgtggcg gtccgcttcc cggttgcaaa
    11161 agaactgggg gcgacggcag tagtaaatgg ttctaccgaa gatgtggtgg cgcgctgcca
    11221 gcaaatttgt ggcaaagata atctgggact ggtgattgaa tgctccggtg ccaatatcgc
    11281 actgaaacaa gccatcgaaa tgctccgccc gaatggggaa gtggtacgcg ttggaatggg
    11341 cttcaaacct cttgatttct cgattaatga cattaccgcc tggaataaaa gcatcattgg
    11401 gcatatggcc tatgactcca cctcatggcg taacgctatc cggctattag ccagcggcgc
    11461 tatcaaagtc aaaccgatga tcacgcatcg tatcggcctg tcgcaatggc gcgaagggtt
    11521 tgatgcgatg gtcgataaaa ccgcaatcaa agtgatcatg acttacgact ttgatgaata
    11581 actactctcc tgaaggaacg ttatcgcatg gaacagataa caaaaccgca ttgtggtgca
    11641 cggctggatc gcttaccgga ttgccgctgg cattcgtcaa tgtttgccat cgtcgcgttt
    11701 ggcttgcttg tctgctggag taatgccgtt ggtggcttga tcctcgcgca gctgaaagcg
    11761 ttgggctgga cagataattc caccacagcc acattctcag caatcacgac cgccggaatg
    11821 tttctcggtg cgctggtggg cggcatcatt ggcgacaaaa ccggtcgcag aaatgcgttc
    11881 atcctctatg aggccattca tattgcctcg atggttgtcg gtgctttctc accgaatatg
    11941 gattttctta ttgcctgccg ttttgtgatg gacgttgggc tgggagcttt actggttaca
    12001 ctgtttgctg gtttcaccga atatatgccc ggtagaaatc gcggaacgtg gtccagtcgg
    12061 gtttccttta ttggcaactg gtcatattcg ctctgctcac tgattgcgat gggactcacg
    12121 ccgctgatta gtgcagagtg gaactggcgg gtacaactgc ttattcctgc aatattgtcg
    12181 cttatcgcta cggcgctggc ctggcgctac tttcctgaat ccccgcgctg gctggaatcg
    12241 cgcggacggt atcaggaagc cgagaaagtc atgcggagta tagaagaagg cgtcatacgc
    12301 cagacgggta aacctttgcc gcctgtggtt attgctgatg acggtaaagc gccacaagcg
    12361 gtgccgtatt cagccttact gacaggagta ttactgaaac gcgtgatatt aggctcttgt
    12421 gtgctgatta ccatgaacgt tgtgcagtac acactaatta actggttgcc aacaatattc
    12481 atgacccagg ggattaattt aaaagactcg attgttttaa ataccatgag tatgtttggc
    12541 gcgccatttg gtatttttat tgccatgctg gtgatggata aaattccgcg taaaacaatg
    12601 ggtgtggggc tattaatcct gattgcggtg ctcggatata tctattcact gcaaaccagt
    12661 atgttgctca taacgctgat tggtttcttc ctgattactt tcgtctatat gtacgtttgc
    12721 tatgcctcgg cagtgtatgt ccctgaaatc tggccgacag aggccaaact ccgtggctcc
    12781 ggtctggcga atgcggtagg gcgaatcagt ggtattgccg caccttatgc cgttgcagtg
    12841 ctgctcagta gttatggtgt aacgggagtc tttattcttc tgggggctgt ttcaattatt
    12901 gtcgcaattg ctatcgccac cattggaatt gaaaccaaag gtgtctccgt tgaaagttta
    12961 agtattgatg cagtagccaa taaataatac gggtggattc aaaatgaaaa attcaaaagc
    13021 aatattgcag gtgccgggca caatgaaaat tatttcagca gaaataccag tgcctaaaga
    13081 agatgaagtt ttgattaaag tagaatatgt cggtatttgt ggttcagatg tacatggttt
    13141 tgaatcaggc ccgtttattc cgcctaaaga cccaaatcaa gaaattggcc tgggtcatga
    13201 atgcgccggg acggttgtgg ctgtgggaag ccgcgtgcgc aaatttaaac cgggggatcg
    13261 ggtaaatatc gaacctggcg ttccttgcgg tcactgtcgt tactgtctgg aaggcaaata
    13321 taacatctgc ccggacgttg attttatggc gacacaaccc aactaccgcg gcgcattaac
    13381 gcactatctg tgtcatccgg agagctttac ttacaaactg cccgacaata tggacacgat
    13441 ggaaggggcg ctggtggagc ctgccgcagt cgggatgcat gccgcgatgc tggcagatgt
    13501 taaaccgggt aagaagataa ttattctggg agcaggttgt attggtttga tgacgttgca
    13561 agcgtgcaaa tgcctgggcg caacggaaat tgccgtcgtt gatgtactgg aaaaacgtct
    13621 ggcaatgggg gaacagcttg gtgcgacagt ggttattaat ggcgcaaaag aggacactat
    13681 tgcacgctgt cagcaattta ccgaagacat gggcgcagat attgttttcg aaacagcggg
    13741 ttctgcggtc accgttaaac aggcaccttt aatctggtaa tgcgcggcgg taaaattatg
    13801 attgttggta ctgtacccgg cgattcggca atcaatttcc tcaaaatcaa tcgcgaagtc
    13861 actatccaga cggtattccg ctatgccaat cgttatccgg tcacgattga agctatttct
    13921 tcagggcgat tcgatgtgaa atcgatggtg acgcatattt acgattatcg ggatgtacaa
    13981 caggcatttg aagagtcagt taacaacaag cgcgacatta ttaaaggcgt tattaaaatt
    14041 agcgattaat taatacggga gaacagaaca tgctcgcaga tatcagatat tgggaaaacg
    14101 atgccacaaa taaacattat gcaattgccc attttaacgt atggaatgca gaaatgttga
    14161 tgggcgttaa agacgcagcc gaagaagcga aatccccggt tattatttct tttggtacag
    14221 gttttgtcgg taacacctca tttgaagatt tctctcacat gatggtatca atggcacaaa
    14281 aagcaacggt gccggtaata actcattggg atcatggtcg gagtatggag attattcata
    14341 acgcctggac tcatggtatg aattcattaa tgcgtgatgc ttctgcattt gatttcgaag
    14401 aaaatattcg tttaaccaaa gaggctgttg atttctttca tccgctgggt attccggtag
    14461 aggcggaatt agggcatgtc ggtaatgaaa ccgtttatga ggaagcgctg gcgggctatc
    14521 actataccga tcctgaccag gcggctgaat ttgttgaacg tacgggctgt gactcattgg
    14581 ctgtcgccat cggcaaccag catggggttt atacgtcaga gccgcaattg aactttgagg
    14641 tcgtcaaacg cgtacgcgat gctgtttctg ttccgctggt tttgcatggc gcatcgggga
    14701 tcagtgatgc cgacattaaa actgcaattt cgttgggtat tgcgaaaatc aacattcata
    14761 cggagctctg tcaggccgca atggtcgcag tgaaagaaaa ccaggatcag cctttcctgc
    14821 atttagaacg tgaagtacgt aaagctgtaa aagaacgggc attggaaaaa attgaactgt
    14881 tcggttctga tggcaaagcg gaataatgat atggataatc tcgacgttat ttgtataggt
    14941 gccgctattg ttgatattcc attacaaccg gtcagtaaaa atatctttga tgtagattct
    15001 tatcctcttg aaagaatcgc aatgagcacc ggggggcgat gcaataaatg aagcaacaat
    15061 tatttctcgt ctggggcatc gtaccgcatt aatgagccgc attggtaaag atgcctcagg
    15121 gcaatttatt ctcggccatt gtcgcaaaga gaatattgat attcaaagcc tgaaacagga
    15181 cgtcagcata gatacatcta ttaacgttgg actggtgacg gaggatggcg agcggacgtt
    15241 tgtcacgaac cgtaacggca gtttgtggaa attaaatatt gacgatgttg attttgcgcg
    15301 gtttcctcag gcgaaattat tatcactggc cagtattttc aacagtcctc tattggatgg
    15361 taaagcgcta acagaaattt ttacgcaagc gaaagcccgg cagatgatca tttgtgccga
    15421 tatgatcaaa ccgcggttga atgaaacgct ggatgatatt tgcgaagcat tgagttatgt
    15481 cgattatctg tttccaaatt ttgccgaggc aaaattactc acagggaaag agacactgga
    15541 tgaaattgct gactgctttc ttgcgtgcgg cgtaaaaacg gtggtgatta aaacgggtaa
    15601 agacggctgc tttatcaagc gtggtgacat gacgatgaag gtgccggcgg tcgcaggaat
    15661 aaccgccatc gacaccattg gcgcgggcga taactttgct tcaggtttta ttgcggcact
    15721 gttagaaggc aaaaatctgc gtgaatgcgc acgctttgcc aatgcaacgg cggctatctc
    15781 ggttctaagc gtcggtgcca ccaccggcgt aaaaaacaga aagctggtcg aacaattgct
    15841 ggaagaatac gaaggataat gaaggcaaat gaaaaagata cctttaggca caacggatat
    15901 aacgctttcg cgaatggggt tggggacatg ggccattggc ggcggtcctg catggaatgg
    15961 cgatctcgat cggcaaatat gtattgatac gattcttgaa gcccatcgtt gtggcattaa
    16021 tctgattgat actgcgctag gatataactt tggcaatagt gaagttatcg tcggtcaggc
    16081 gttaaaaaaa ctgccccgtg aacaggttgt agtagaaacc aaatgcggca ttgtctggga
    16141 acgaaaagga agtttattca acaaagttgg cgatcggcag ttgtataaaa acctttcccc
    16201 ggaatctatc cgcgaagagg tagaagccag cttgcaacgt ctgggtattg attacatcga
    16261 tatctatatg acgcactggc agtcggtgcc gccatttttt acgccgatag ctgaaactgt
    16321 cgcagtgctt aatgagttaa aggctgaagg gaaaattcgc gctataggcg ctgctaacgt
    16381 cgatgctgac catatccgcg agtatctgca atatggtgaa ctggatatta ttcaggcgaa
    16441 atacagtatt ctcgaccggg caatggaaaa cgaactgctg ccgctatgtc gtgataatgg
    16501 cattgtggtt caggtttatt ccccgctaga gcagggattg ttgaccggca ccatcactcg
    16561 tgattacgtt ccgggcggcg ctcgggcaaa taaagtctgg ttccagcgtg aaaacatgct
    16621 gaaagtgatt gatatgcttg aacagtggca gccactttgt gctcgttatc agtgcacaat
    16681 tcccactctg gcactggcgt ggatattaaa acagagtgat ttaatctcca ttcttagtgg
    16741 ggctactgca ccggaacagg tacgcgaaaa tgtcgcggca ctgaatatca acttatcgga
    16801 tgcagacgca acattgatga gggaaatggc agaggccctg gagcgttaaa tactcaacac
    16861 gattcacctc tgtttctgtg aaaactgtga tgaaaatctg tgattcaaat aggttatttt
    16921 gaaagtaaac atcggttatg cgataatcgc gctaatgtga tgtaaaaaga tacaggggtg
    16981 taatcgtggc ggcaaaagac aggattcagg caattaagca aatggttgcc aacgataaaa
    17041 aggtgacagt ctcaaatttg agtgggattt ttcaggtaac cgaagaaacc attcgccgcg
    17101 atcttgagaa gctggaagat gaaggctttt tgaccagaac ctatggtggt gctgttttaa
    17161 atacagcggt gttgacggag aatatccatt tttataagcg cgcttcatcg ttttatgaag
    17221 agaagcagct tattgcacgc aaggcactac cctttatcga caataaaacc accatggcag
    17281 ccgattccag tagtaccgta atggaattgc tcaaattgtt acaggaccgt agtgacctga
    17341 cgttgctaac caactcggcg gaagcgattc atgttctggc tcagtcagaa attaaagtcg
    17401 tttcaacggg tggggaacta aacaaaaata cactttccct gcaaggaaga attactaaag
    17461 agatcatcag ccgctaccat gtcgatatca tggtaatgag ttgtaaaggt cttgatatta
    17521 acagcggcgc gctggactct aatgaagcgg aagctgaaat caaaaagaca atgatccgtc
    17581 aggcgacaga agttgcgtta ttggttgatc actctaagtt tgatcgtaaa gcttttgtcc
    17641 agttagctga ctttagtcat attaattata taataactga taaatcaccg ggtgcagagt
    17701 ggatagcatt ttgcaaagac aataatatcc agctggtgtg gtaatttaag cacacgttgt
    17761 caatattatt tgttcctgcg cctggaaata aaatgatgat aaagatgcgg caagtgaatt
    17821 tgccgcaatt gctacctttt atagcaaatc cgaggcaccc aatggaacaa tatgatcaaa
    17881 ttggcgcaag actggaccgc ttgcctttgg cccggtttca ttatcgtata tttggtataa
    17941 taagctttag tctgttatta acggggtttt tgagttactc aggtaatgtc gtcttagcaa
    18001 agctggtaag caatggatgg tcaaataatt tcctcaatgc cgcctttacc tcggcattaa
    18061 tgtttggtta tttcatcggc tcacttactg gtgggtttat tggtgactac tttgggcggc
    18121 gcagggcgtt tcgcataaat cttctcatcg tcggtattgc tgcaacaggg gccgcttttg
    18181 tccctgatat gtactggctc attttcttgc gcttcctgat gggaacagga atgggggcgc
    18241 tgattatggt tggctatgcc tcatttacgg agtttatccc cgcgacggtg cgtggaaaat
    18301 ggtccgcgcg gctctcattt gttggtaact ggtcgcccat gctgtctgcg gcgataggcg
    18361 tggtggttat cgcttttttt agttggcgaa taatgtttct tctgggtggt attggcatac
    18421 tgttagcctg gtttctctca ggtaaatact ttatcgagtc gccacgatgg ctggcaggga
    18481 aagggcaaat cgccggtgca gaaagccaac ttcgtgaagt agagcagcaa attgaaaggg
    18541 agaagagtat tcgtttaccc cagcttactt tgaaccagag caacagcaag gttaaggtaa
    18601 tcaagggtac cttcaggctc ctgtttaaag gggaaatgtt acgacgtaca ttagttgcga
    18661 taactgtttt aattgcaatg aacatttcgc tttataccat taccgtatgg ataccgacca
    18721 tatttgttaa ctccggcatt gatgtcgata aatcaatatt aatgaccgct gttattatga
    18781 ttggtgctcc ggtaggaata tttattgcgg cattaattat tgatcatttt cctcgtcgat
    18841 tatttggctc cgccttactt attattattg ccgtgttagg ctatatctat tcaagtcaga
    18901 ctacagagtg ggcgatttta atctatggtc tggtgatgat cttcttttta tacatgtatg
    18961 tttgcttcgc gtcggcggtt tatatcccgg agctttggcc aacgcattta cgcctgcgcg
    19021 gttcgggttt cgttaatgcc gtcggacgga tcgtcgcagt tttcacgccc tatggcgttg
    19081 cggcattatt aacacattat gggtcgatca cggtgtttat ggtgcttggt gtcatgttag
    19141 tgctctgtgc gctggttctc tccatttttg gcatcgaaac gcggaaggtg tcgctggaag
    19201 agatttctga ggtgaattaa atctttcgtt gatggcatca attgccagat gtaccactga
    19261 cgtgttttat ttggcctaca atgccagtac agaattgtag gccagcgtaa aattacccct
    19321 gcgtctcttc ccagtctgcc agcgtatata gcgttgcccc agctgctgac atctccataa
    19381 acgcgtgtgc actgtcctgg ggctggatat tcacgccacg acagccatcg gtaatcacgt
    19441 ttaccttata acctaactgt aacgcgtcca gcacggtaaa cttcacgcaa tagtcagtag
    19501 ccaggcccat aacgatcaat tcatcgattt catgatcgcg caaccagtca tcaagcgcgg
    19561 ttttctgccg acgtccgtta tcaaaaaagg cactgtaact gtcaactaaa ggattttcgc
    19621 ctttatggaa caccgctgcg atctcttttt gattcagtaa cggatgtaat tgtgcgcctt
    19681 cactgttctg cacacagtgt tcaggccaga aagtttgccg caggccgtcg agttggcctg
    19741 gcgtataagg ctctacgccg tgctggctgg caaagctgcc gtgattcgcc ggatgccagt
    19801 cctggctggc gataaccgct tctccgcgcg actggcacca gtcaataagg cggttagcaa
    19861 cctccaccgt actgtcacct tccggtacgg cgagcgcgcc accagcacag aaatcatttt
    19921 gtaaatcgac cagtaacagg gcgcgatggg gcattgcagt ctccttaatc atccagcgtc
    19981 agttcgccgc gcaggttttg gctcatggcc ttgcgaatgg tttcagtatc cagttcctgg
    20041 ctcagcaggt aatgcagttt ggttagcgtg gcttctacag tcatatctgc accgccaata
    20101 acgccggcat gggcgaggac gttaccggtg gcataaccac tcatattcac tttaccggac
    20161 atacattgtg tcaggttgac caccacaata ccgcgatcgc tggcttcttg taattcctgc
    20221 aggaaggctt tgttttgtgg cgcattaccc acgccatagg agcgcagaat caatgctttc
    20281 accggttggc gcagaaaatt gcgcacgacg tcagcagaaa tccctggata aatcgtcact
    20341 acgccaattg gttgtggcgt gattggatga acgattaatt ccccttcacc gtgcggggcg
    20401 ggtggcgtat tcaaacgacg aatatggata ccagcttcca gtaacggagg aaggtttgga
    20461 gaggcaaacg catcaaaacc atcggcatgg gctttggtag tgcggttgcc gcgatacaat
    20521 cggttattga aaaagagcgt tacttcgttg atcggataat tcgccgcaac gtacaacgca
    20581 ttcagcagat taatttgtcc gtcagagcgt aactcagcca gcgggatttg tgaccctgtc
    20641 acaatgaccg gtttaccgag attctcgagc atgaacgaca gcgcagaggc ggtatacgcc
    20701 atcgtgtcgg taccgtgtag aatgacaaaa ccatcatagt cgtcatagtg cgctttaata
    20761 tcttcagcaa tatgctgcca gtcttccggc gtcatatcag aagaatccat caacggcgta
    20821 tattcatgaa tggtgaaatc tggcatctcc aggcgatgga attccggcat cagcgccagt
    20881 tggcgttgta gatgacctga caccggaata taaccctgct cggaacgctg catcccgatg
    20941 gtcccgcccg tgtaggcgac gtaaattgat ttcttttgca tgatattgat atgaatgata
    21001 tcgaaagagc aaaagtatat acgtgaggca ggaggagcgc acctgatact atgcggataa
    21061 ggaaatatgg ggcgagacag cgggaagagg attcgtcgag gtaaaaaata atgtggccga
    21121 cgatctgtcg gccactcagt acaagactta acgcacgttg gcgcaggtca ggcaaaacgc
    21181 ataacggttt tgtgggtcgt taaacgcggc cagcttgtcg ctttcacttt taacggtaga
    21241 ggctaccgag gcaagcggtg caggtaacat ggcctggaac gcatctggca acattgcccg
    21301 gacagaacca gacatgttgt ccatcacttt gtcgaagaag gtcggttcat caacgtagta
    21361 ttccagatgc cactgtttca cttttgcgag ctctgctgct ttggcaaccg catcatcgaa
    21421 atccccgaga ctatcgacca gcccgttagc ttttgcatcc tgaccggtcc agacgtggcc
    21481 ctgggcgatt ttatcaattt gctccggcgt cgaatgacgc gcatcagcaa ccagcgtgat
    21541 aaagcgttta tagccattct caatgcttaa ttgcatcatc tgctgcgctt ccggcggcag
    21601 tgccctggtg atagaaacat ccgccagcgg tgaagttgag acaccatcag tatgaacacc
    21661 aatcgaatcc agactatttt ctacggtggt gatcacgccg aagataccga tagaaccggt
    21721 cagggtgctg gggttagcca caatgtaatt agctggcgtg gaaatccagt aaccaccaga
    21781 tgccgccatg ccgcccatcg atacaaccac agacttaccc gctgcccggg ctgctgccag
    21841 ttcagcgcga atcacttcag acgcggtaac gctgccgcct gggctattaa cacgcaggac
    21901 aatcgctttc actttcgggt caaggcgagc gtcgcggatt tgtgccgcag tggtatcacc
    21961 gccaacattc ccctgagttt cctcgccatc cataattgcg ccattagcga agacgacacc
    22021 gatgctgtca ccggtatctg ccggcgtttt caatgcgtaa tcgtaataac tgatggcgcg
    22081 ataattttta tcagtcttac tccagccgaa ctctttggtc agagcttttt cgatttcggc
    22141 actcgatgcc agtgcatcga ccagcttgtt ttccagtgca tatttcgcgg tatcgccacc
    22201 ggttttggtt aaaccctcaa gcaacccttg cgcgccaggg aatacctgct gagcagggat
    22261 ctgccggtta gcggcaacag tattcagata gttttgccac agctcaccaa tccagcggct
    22321 gtcagcttcg cgggctgccg gtgacatatc atcacgaata aacggttcaa cggcagattt
    22381 atacgtaccc acgcggaaca catgggtgga aactttcagc ttatccagca acgatttgta
    22441 gtacagaccg ttggtagcaa aaccgtgcag atcaaccacg ccttgcggtg acagccaaat
    22501 tttattggcg aaactggcga gataatattg cccctggctg tagttctcgc caacggcata
    22561 aaccggtttc ccgctgtcac gaaactcttt cagggctttg ccgatgtact gcatagacgg
    22621 ctggtcgccg cctgcgaagt ttttcagatc catcacgata ccggtgatat tgcggtcgtc
    22681 cttcgcctgg cgaatggtgt tgacgatatc aaacagtgag ttttcctgca gacgatcgga
    22741 actggcacca agcagctggc ggcttaattt actaaaccgc tgagaactgt ctggtttatc
    22801 gacgatcaca ccagaaatgt ccagcagcag tgcgccacga ctggccgttt ctttcgaatc
    22861 accaccactg acctgcatcc agatccccac accaaccagt acgaggaaaa taaagaacag
    22921 gttaagtacc atttcacgga cgaaattcag cagacgccac gtccatttaa aaaatccggc
    22981 aataaatcgc caaagggttc gcatgtattc tcccaactta aggtcacacc tgtcacaggc
    23041 gcaatagatg tggttatcgt aatgacccaa ccggcacttg tcagcaggaa ttgcggactg
    23101 tgctgtaaca aaatccactc tcgtgttaat tttgtgaata aatatcacga caggagttaa
    23161 tcaaatggat gcactcgaac tattgatcaa tcgccgtagc gcctcccgct tggctgaacc
    23221 cgcgccaacg ggtgaacaac tgcaaaacat cctgcgtgcg ggtatgcgtg cgccggacca
    23281 taagtccatg caaccgtggc atttttttgt gattgaaggg gaagggtgcg agcgtttcag
    23341 cgccgtactg gaacaggggg cgattgctgc cggtagtgat gataaagcta tcgacaaagc
    23401 ccgtaatgcg ccgttccgcg caccgctcat catcacggtg gtggcgaaat gcaaagaaaa
    23461 tcataaagtc ccgcgatggg aacaggaaat gtctgccgga tgcgcggtca tggcgatgca
    23521 aatggcagcc gttgcccagg gatttggcgg catctggcgc agtggcgcat taactgaaag
    23581 tccggtagtg cgtgaagcat tcggttgccg tgagcaggat aaaattgtcg gttttctcta
    23641 cctcggcacg ccgcagttga aagcatctac gtcgattaac gtcccggacc cgacgccgtt
    23701 tgtaacttat ttctgataat tgtcgcgaaa ctgtctggat tgtgagcaaa ggcgcaggaa
    23761 ttcagacact ctcacttatc acttcacgga atgagggtta ccatacccgg attgcattga
    23821 caggagatgt ccatgagcga gaactcgatt cgtttgaccc aatacagcca cggagctggt
    23881 tgcggctgta aaatttcccc aaaagtgttg gaaaccatcc tgcacagtga gcaggcgaag
    23941 tttgttgatc cgaatttgct tgtgggtaat gaaacccgcg acgatgcggc ggtgtacgat
    24001 ctgggcaatg gcaccagcgt tatcagtacc accgacttct ttatgccgat cgttgataat
    24061 cctttcgatt ttggccgcat tgcggcgact aacgccatca gcgatatctt cgcgatgggg
    24121 ggcaaaccga ttatggcgat tgcgatcctc ggctggccga ttaacaaact ttccccggaa
    24181 attgcccgcg aagtaaccga aggtggacgc tatgcatgcc gtcaggcggg tattgcgctg
    24241 gctggcggtc actccatcga tgcgccggag ccgatttttg gtctggcggt aacggggatc
    24301 gtaccgaccg agcgggtgaa gaaaaacagt accgcacaag ccggatgcaa actgttcctg
    24361 acgaaaccgc tggggatcgg cgttcttacc acggctgaga aaaaatcact gttgaaacca
    24421 gaacatcagg gactggcgac ggaagtgatg tgccggatga acatcgcagg cgcgtccttt
    24481 gccaacatcg aaggcgtaaa agcgatgacc gacgttacgg gctttggtct gctgggccac
    24541 ttgagcgaaa tgtgtcaggg ggctggtgtg caggcacgcg tcgactatga agcgatcccg
    24601 aaactccccg gtgttgaaga gtacattaag ttgggcgcag tacctggcgg cactgaacgt
    24661 aactttgcca gctacggtca tctgatgggt gaaatgcagc gtgaagtgcg cgatctgctg
    24721 tgtgatccgc aaacttctgg cggtttgctg ctggcggtca tgccggaagc agaaaatgag
    24781 gttaaaacta cagccgccga gtttggcatt gaactgacgg cgattggcga actggtgcca
    24841 gcgcgcggcg gtcgtgccat ggttgagatc cgttaattca atgcggttgt ttattgccga
    24901 aaaaccgagt ctggcgcgcg ccattgctga tgtcctgccc aaaccgcacc ggaaaggcga
    24961 tggctttatc gagtgcggta atggtcaggt ggtgacttgg tgtatcggtc acctgcttga
    25021 gcaggcgcag ccagacgcct acgacagccg ctatgcgcgc tggaatcttg cggatttgcc
    25081 gattgtcccg gaaaagtggc aattacagcc acgaccctcc gtgaccaaac aacttaacgt
    25141 catcaaacgc ttcctgcatg aagccagcga aatcgttcac gccggggacc cggatcgtga
    25201 agggcaattg ctggtggatg aagtgctgga ctatctgcaa ctggcaccgg aaaagcgcca
    25261 gcaggtgcag cgttgcttga taaacgacct gaacccgcag gcggttgagc gggcgatcga
    25321 ccgtcttcgt tccaacagtg agtttgtgcc attgtgcgtt tctgcgctgg cgcgagcgcg
    25381 tgccgactgg ctgtacggca tcaatatgac ccgtgcgtat accattctcg gtcgcaatgc
    25441 cggttatcag ggcgtacttt ccgtgggacg cgtgcagaca cccgtgctcg ggctggtggt
    25501 gcgccgcgat gaagagattg aaaacttcgt ggcgaaagac ttctttgaag tcaaagcaca
    25561 tatcgtgaca cctgccgatg agcggtttac cgctatctgg caaccgagcg aagcgtgtga
    25621 accgtaccag gatgaagaag ggcgcttgtt acatcgtcca ctggcggagc atgtggttaa
    25681 ccgcattagt ggtcaaccgg ctattgtcac cagctataac gataaacggg aatcagaatc
    25741 cgcgccgctg ccgttttcgc tttcggcgtt gcagattgaa gcggcaaaac gcttcggtct
    25801 gagcgcgcag aacgtgcttg atatctgcca gaagctgtac gaaacgcaca agctaatcac
    25861 ttatccgcgt tccgattgtc gctatttgcc agaagaacat tttgccggac gccacgcggt
    25921 gatgaatgcc atcagcgttc atgcaccgga tctgttgccg cagccagtgg tagatccaga
    25981 tatacgcaac cgctgttggg atgacaaaaa ggtcgatgcg caccacgcca tcatcccgac
    26041 cgcacggagt tctgcgatca acctgacgga gaacgaagcg aaggtctata acctgattgc
    26101 ccgtcaatac ctgatgcagt tctgcccgga tgcggtgttc cgcaagtgtg ttatcgaact
    26161 ggacattgcc aaaggcaaat ttgtcgctaa agcccgtttt cttgctgaag caggctggcg
    26221 cacgctgtta ggcagcaaag agcgcgatga agaaaacgac ggcacgccat tgcctgtggt
    26281 ggcgaaaggc gatgagttgc tgtgtgaaaa aggtgaagtg gtagagcggc aaacccagcc
    26341 gccgcgccat tttaccgatg caacactgct ttcggcgatg accgggatcg cgcgctttgt
    26401 gcaggacaaa gatctgaaaa aggtccttcg tgcgaccgat ggtctgggga cagaagcaac
    26461 ccgtgccggg attattgaac tgctgttcaa gcgtggtttt ctgaccaaaa aagggcgcta
    26521 tatccactcc accgacgccg gaaaagcgtt attccattcg ctgccagaaa tggcgacgcg
    26581 accggacatg accgcgcact gggaatcggt gctgacgcaa atcagcgaaa agcagtgtcg
    26641 ctatcaggac tttatgcagc cgctggtggg gacgctatat cagcttattg atcaagccaa
    26701 acgtacgccg gtgcggcagt ttcgcggcat tgtggctccg ggcagtggtg gcagtgctga
    26761 taagaaaaag gctgcaccgc gtaaacgtag tgcgaaaaaa agtccgccag cagatgaagc
    26821 cggaagcggg gcgatagcgt aagcgagcga atctttcgtg ctattcgagt catattctga
    26881 aatatccagc ggatcaagaa aattcgttgg atattttttt gcatggataa aattatcgcc
    26941 tctaaagtat gtaataacag ggaatgtgat gaaaaaagtt ttattacaaa accatcctgg
    27001 gagcgagaag tattctttta atggctgggg aatatttaat agtaattttg aacgggtgat
    27061 taaagaaaat aaggccatgc tgctttgtaa gtgggggttt tatttaacat gtgttgtcgc
    27121 tgtaatgttt gtattcgcag cgataacatc caacggtttg aatgaaagag gcctgattac
    27181 cgcgggatgc tcttttcttt atctattaat tatgatgggg cttattgttc gggccggttt
    27241 taaagcaaaa aaagaacaac tacattatta tcaggctaaa ggtattgagc cgctcagtat
    27301 cgagaagtta caggcgctac aattgatcgc accttatcga ttctatcata agcaatggtc
    27361 tgaaacgcta gagttctggc cgcgaaagcc tgaacctggc aaagatacct tccaatatca
    27421 tgtgcttcct tttgactcga tcgatatcat cagtaaaaga cgcgagtctt tagagaatca
    27481 attgggtatc gaagatagcg aaagttattg tgccttaatg gagcattttc tttctggcga
    27541 ccatggagcc aataccttta aagcaaacat ggaggaagcc ccagagcagg ttatcgccgt
    27601 gttgaataaa tttgctgttt ttccctcaga ctatatctct gattgcgcta ataatcgctc
    27661 cggtaaatcc tcggcgaagc taatatgggc ggcggaatta tcatggatga tctcgatatc
    27721 aagcacagct tttcaaaacg ggacaattga agaagaactg gcctggcatt atataatgct
    27781 tgcttctcga aaggcgcacg agttgttcga aagcgaagaa gattatcaaa aaaatagtct
    27841 aatgggattt ctttactggc atatctgctg ctatcgcaga aagttaacgg atgctgaact
    27901 tgaagcgtgt tatcgttacg acaagcagtt ttgggagcac tacagtaaga aatgccgttg
    27961 gcccataaga aatgttccct ggggagcatc atccgttaaa tactcataaa cgcctgaaat
    28021 tttgccgggg gcgctgcgct tgcccggcct acattagagc gtaactgcaa tgaattgtgc
    28081 ccatctgtag gcctgataag cgtagcgcca tcaggcattt acaacttaaa tcacaccctg
    28141 cgccagcatc gcatcggcaa ccttcacaaa accggcaatg ttcgcgccct gcacgtagtt
    28201 ggtttgctca ccttcaccac catgctcaac acaggcatgg tggatatcca gcatgatgtg
    28261 atgcaaacgt gcgtcaactt tctcggcttt ccagcccagg cgcgcagcgt tttgtgccat
    28321 ttccaggccc gacgttgcta caccgccagc attagccgct ttacccggtg caaacagtac
    28381 gcctgcctgc tggaacagtt cagtcgcttc gatggtggtc ggcatatttg ccccttcagc
    28441 gacggcttta acgccattgg cgataagctg atgcgcggtg tcaacatcca gttcgttctg
    28501 ggtggcgcaa ggcagggcga tatcaaccgg tacagaccac ggctgttggc cttcgagata
    28561 gaccagacca aattctttgg cgtaatctgc cactcgacca tcgcggctgg ctttgatttc
    28621 gataagacgt gccagtttct ctttcgtgaa tccgctttca tcaactacag tgccgctgga
    28681 gtctgacgca gtgatcacac gagcaccaaa ttccatcgct ttttcgatag cgtactgggc
    28741 gacgttgccg gagccagaaa cggaaacgcg catcccttca aaacccatac cgtggcgttt
    28801 taacattgct tctgtgaaat aaaccagacc gtagccggta gcttccgggc gaataagact
    28861 accgccaaat gaaaggccct taccggtgaa gacgcaggcg gtattgttgg agagcttttt
    28921 catcatcccc gccataaagc cgacttcacg accaccaacg ccgatatcac ctgccggaac
    28981 gtcggtatcc gcgcccaggt ggcgatacag ttcagtcatc agcgcctggc aaaaacgcat
    29041 cactttacct tcgctttttc ctttcggatc gaaatcgctg ccgcctttac caccgcccat
    29101 cggcagggta gtcagggcat ttttgaaggt ttgttcaaag ccgaggaatt tgagaatgga
    29161 aaggttaact gacggatgga agcgcatacc gcctttgtac gggccgatgg cagagctgaa
    29221 ctgcacacgc catgcacggt tgacctgtat ctggttgcga tcatcaaccc ataccacgcg
    29281 aaactggatc acgcgctccg gttcaaccag acgctccagt aatgacatct ggcgatattt
    29341 tggattttgt tcaagaaaag gccagagtgt ggtcattact tcacgaacgg cttgcgcgaa
    29401 ctcggtttga ttcgggtcgc gcttttggac atggttgagg aatgactcca gagaatatgt
    29461 ctgatccata gatataaaaa ccttatatat taatacgatt gtgcttatgt tgtttatgtc
    29521 atgtgctttt gcagttttcg actataccat caagaatgtg acggtaagca agaaaaaaat
    29581 gacccaggaa agcaaattaa taacgagagt aatctcataa ccaaatgcga taactcagac
    29641 gctaaagttt tgccacccgg ctaccgttat cagtagcaga gacaataacg ttaaggagtt
    29701 ccctgtgagt cgagcattgt tcgccgtagt attagcattt ccattaattg cactggctaa
    29761 cccgcattat cgtccggacg tggaggtaaa cgtaccgcca gaagtgttca gttcaggcgg
    29821 gcagtcagcg cagccctgta ctcagtgctg cgtttatcag gatcaaaatt attctgaagg
    29881 ggcggtaatt aaagccgaag gaatactgct gcaatgccag cgtgatgaca aaacccttag
    29941 cactaatccg ctgctctggc ggcgcgtaaa gccataaacg cctctaataa tggaatgtca
    30001 gcaggggcca gcggatattg cagcgcctct tcaggtgagc accagaccag cgcctgatgt
    30061 tcatgtgcct gtaacgtccc gtggaagtcg ggtacgtgcc aggcatgaag atggataatc
    30121 cggcccgaaa cttctcgctg atggctggca acatactcac ccacagttgc ttcgatgtcc
    30181 aattcttcgt ttaactcacg caccagcgcc tgctgctggc tttcatccag ctcgacttta
    30241 cctccggcaa actcccataa tcccgcctga tcgctctggg cggggcgttg cgcgagtaaa
    30301 attttgccat cacgttcaat gatggcggca acaacttcaa tcattttcat aaattacaca
    30361 cgggcagata agcgaaaaat gccataatat ccgcactgtt gctcaatcag aaaggcttta
    30421 tatttaagga gtgcctgtgc tagaccgcca tcttcatccc cggattaaac cgttgttgca
    30481 tcagtgcgtg cgggttcttg ataaatcggg cattacgcca gatggtttaa cattagtggg
    30541 atttgccatc ggcgtgctgg ctctgccgtt tctggcgctg ggctggtatc tggcggcgct
    30601 agtcgtcatt ttgttgaaca ggctgcttga tggtctggac ggtgcgctgg cgcggcgcag
    30661 agggcttacc gacgcgggcg gctttcttga tatttctctc gattttctct tttacgcgct
    30721 ggtgccgttt ggctttattc ttgctgcacc ggagcaaaat gcgctggcag gcggctggtt
    30781 gttgtttgcg tttatcggca cgggcagcag ttttctcgcc tttgctgcac tggcagcaaa
    30841 acatcagatt gataaccccg gttatgcaca taagtcgttt tactatctgg ggggattaac
    30901 cgaaggcaca gaaacgatct tactgtttgt gctgggatgt ttattccctg catggtttgc
    30961 gtggtttgcg tggtttgcgt ggtttgcgtg gtttgcgtgg atcttcggtg cgttgtgctg
    31021 gatgacgacc tttacgcggg tgtggagtgg gtatctgacg ctgaagtcgc tccagcgtca
    31081 gtaattgcaa tgttatttgc tactgcccgg gccgcgttca ccggttgcaa ccggattttt
    31141 tggatcgctg ctccattcgt accagccgcc gtcatacacg gaaacattct tccaacccat
    31201 ggcgcgtgcg tacataaagg tttcggacgc gcgccagccg gtgccgcagt agaatgaaac
    31261 ttgctgatct ggtttgatat tccatgcttt ccacatagcg gtaatatcat cggcgctacg
    31321 catggtgcca tccgggttat ggaaatcttc catatgcgtc gagtcgctac cagcgtgtcc
    31381 ccaacgtgct ccggctattt caccttttgg tttaatatag ctgtaaccgc tggtcgtacc
    31441 gataaattct ggccacgaac gaatgctcac cagcgatgca tcctggcgat gcagcagtcc
    31501 acgggcctgt tccatatcga gcatcaactg cggttgtgcc gggatcttca cgccgaaatc
    31561 tggttccgct ttcactttcg gtggcgttcc tcgctcaaca ggcagtcccg cgtcggacca
    31621 ggtttgccag ccgccatcca gcagacgcac atctttcacg cctgcgtaga gcataatttg
    31681 cgccacacgc gctccagcgt atacgtcacg cccgtacaga atgaccgtgg tgtcatggcg
    31741 aatgccgtgt tttgccagca tcgcttttag ttgttcatca gaaactttgt tccacagcgg
    31801 ttcgctttcc acttcgttgg tatcgatgta gtcagcgcca ggaatatggc tgataaggta
    31861 aagcttagga gcgccccagg ccgcttcaat gactttccag ccaccgacag gtttcgccgt
    31921 aacctcttta ccttgttgca ggtcatgcag ccattgcgga taaaccagct gctcaaagtg
    31981 cggcagtttt tgtagacggg aaggttcgct tagcgcgtca ctcagaatgg agatatgcgt
    32041 aaaacctgct ttttgcagtc gcgttttgac ggcgtcgaca tctttgccat taccgtacag
    32101 cgccaccggg gcatcggctt tcaggttatg ttgcttgatc cacgcgttga gctgttcggt
    32161 gctcattttg tcgagccagc tagcagagag gtttaaggcg gcaggttcat gaccagaagg
    32221 gccatttaag gtttgtggcc agccgttata aaacgcgctg gggcgagtat ctatcgcttt
    32281 gccattttgt tgctgaagtt ggtcaagtgt aagaggcttc gccagttcag cggcccacga
    32341 agaagcgcaa gccagcccta aagccattgc cagcgcggtc atttgagaaa cacgtttcat
    32401 cgaaaatccc gacattatag ttttgagtgc gccattctga actgacgcat tgagaaaaac
    32461 tttgcgttat cgcagtttgt tgtaattttc tgaccactgc gccatatcca gaacagaact
    32521 atcagcagga acatcctgga gatcgtgcgt tacctgaacg acggggatcg ccagggcgcg
    32581 aacttcgctg aacacccact ggcgaaaatt atcgcgcaga gccacatcaa gacggctgaa
    32641 tggctcatcc aggagtaacg cttttggctg ggcgagaagg gcgcgtagta gagcaacgcg
    32701 cgctcgctga ccgccagaca aagtggcagg atcctgatgg aaagctccgt caaggcctga
    32761 acgctcaagt gcatcattca cggcattacg tcgggcattc cccttaagtg tcgccggtag
    32821 cgccagcagt aaattttgcc cgacgctgaa ctggtcaaat aacagtgcat cctgaaaaag
    32881 aatgccaatc tgacgctgtg cggtgggtag catgtcaatc cgttgctcat tgagccatag
    32941 ctcacctgta caagaaaact gtccggccag tgcaccaatc atccatgaaa acagagtgga
    33001 ttttccacag ccagatggcc ccattaacgt gacgatgtca cctttatcaa ccgtaaagtt
    33061 aacgtttgtc agcaagcggc tttctggtaa acgtagcgaa acatttttca cgcagagcat
    33121 tagcggattc cttgtctgac ataacctacc cattttgcga ctaacgcggt cagggcaaaa
    33181 ataataagcg gtaacaacag ttgccataaa gcctgggcgg cgagaatacc gttgctgccg
    33241 ccgctgctta atgccaccgc ctcagtggtg agcgtcggaa aacgccccgc gcccagccac
    33301 agcgtcggca tatactgcgc aatactgact gaaaatccca ccgcgaaggc aatcagcaca
    33361 gggcgcaaca tgagtgggca tttcacgtaa aagaagattt tggcccgcga ccagcccagt
    33421 gtttgcgcaa tcaaaattaa ccgtgaatca atgcgctgcc aggcgggttg caggataaac
    33481 agcatccacg gcattaccca cagcagatgc ccccagacca ccgccgtcca gcttccatcc
    33541 aggttcagcc atagcgccag cgtgtactag cccgccacca gtggcagagc gggcaataga
    33601 atgggcaacc atagccacaa ctggcgacgc tgtgcccccc attccagcca cagcagtaac
    33661 aggagcaaag cgatgaatgt tgctaccagc cccattgtca gactgttgat gagtgcttca
    33721 ctattgatcg tcgactgatc cgcgaggatc gccagcagaa ccacacacag cacaccggtt
    33781 aagggtaaaa aactcgccag cgtagtgcct ggtaataaag gtgtggcagg cttgcgaatg
    33841 ccatctacgc ggggaatagt gcgccgccag ctgcgccata gcagatagct cagcaaaacg
    33901 taggcggcga gtaacagcat cagcagcaga ctggcgagtg cgcctttcgt ttgttgatcc
    33961 gcgtcgccct gagttaacca ctgccagcta attactgcca gcgtcggtgg attacctggc
    34021 ccaagaataa tcgccacatc cacgaccgaa agcgaccagg caacaatcgc cagcatcgcc
    34081 attgccagcg aaggcgcgac ggagggcaac agcagccagt tcaggcattg ccagcggctg
    34141 tagcccagcg aatccagcac aatgacctgc tgcaacagcc gtttttcgct caatactgcc
    34201 actaagatcc acagcagaaa tgcgctttct ttcactgcaa gggtgaggcc cagaccgatc
    34261 caaatcggtc cattggcgga gtgaaatacg ggaaatagtc atatagcagc cctccgtcag
    34321 caaagagcag cagggcgctg gtggcaaaag ccacatgggg aatggcgagc agccacggca
    34381 gacgggcgca catacgctgc catttcggcc caggccatag cgccacaatc accagcaggg
    34441 caatcaacaa tgccccgacc gccgcgatgg ttgtcgacac cagcgttgcc agtaatgcct
    34501 gcggtaactg tggatcggca aataacgcct gccagcgtgt caacgacaac gcaggcgata
    34561 tcagcgtgag agctgccggg atcagcggtg cataaatcac cgccaccatc gcccacagca
    34621 aaaagattaa tgcgtaccgt aacggcgtaa ccattcttgt tccagtgcat tcacccatcc
    34681 tgcgtgcggt tcagccagta ccggcggcag atcctgcggc attcttgatt gcaatatttc
    34741 gcgctgcccg tcaggcagtt tttgcggatc gagaacagaa ggatcgcccc agacaacggg
    34801 atctgcttta cgcagttgcg catcgggtga gagcaggaaa ttggcaacta ccttcgccgc
    34861 agcactggca ttcgcgttgg ctggaatggt gacgaaatgc acgttgccaa tcatcccctc
    34921 gcgaaagcca aaactgtaac tgcttgccgg caaatcgccg ctggcgattt tttgctgcgc
    34981 atgcgcgggg ttaaaggtca gcgacaggcg caatgtgccg gctttcagca gagcatccat
    35041 ccgcgcgggc gaaggcggga aatcctttcc ttcgcgccac aaatacggat gcagtgcatc
    35101 aagatattgc cacaagggcg cagtgacacg ggcgaaagtc gcatcgtccg gcgcttcttt
    35161 taatgcggcg ggatctggcg tcagcataat cagcaactgt tcaagaaacg ccgtgccggt
    35221 aaagtccggt gggcgcggat aagtaaccgt gccgggattg gctttagcaa actccagtaa
    35281 ggcttgcggc gtttgtggtg gctgtggcgt aacatcgcgg cgggcgataa acgtcagttg
    35341 tgcgccgccc cagggcgatt ccgcaccttg tgtcggcact gaaaaatctt cccgcaccgg
    35401 cagctgtgtg tcgacatagc gccagttggg cagagtctcc gcccagcccg tttgtaataa
    35461 attggcctct tttaagg
//
LOCUS       DAJTNM010000024        35267 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTNM010000024 DAJTNM010000000
VERSION     DAJTNM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.024"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..1127)
                     /gene="setB"
                     /locus_tag="OO172_001342"
     CDS             complement(<1..1127)
                     /gene="setB"
                     /locus_tag="OO172_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5511586.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EIHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFA"
     gene            1495..2625
                     /gene="fruB"
                     /locus_tag="OO172_001343"
     CDS             1495..2625
                     /gene="fruB"
                     /locus_tag="OO172_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5511587.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNCIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2625..3563
                     /gene="fruK"
                     /locus_tag="OO172_001344"
     CDS             2625..3563
                     /gene="fruK"
                     /locus_tag="OO172_001344"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5511588.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3580..5271
                     /gene="fruA"
                     /locus_tag="OO172_001345"
     CDS             3580..5271
                     /gene="fruA"
                     /locus_tag="OO172_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5511589.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5695..6636
                     /locus_tag="OO172_001346"
     CDS             5695..6636
                     /locus_tag="OO172_001346"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5511590.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYVDSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSASKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6624..7562
                     /gene="psuG"
                     /locus_tag="OO172_001347"
     CDS             6624..7562
                     /gene="psuG"
                     /locus_tag="OO172_001347"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5511591.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQIAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7656..8906
                     /locus_tag="OO172_001348"
     CDS             7656..8906
                     /locus_tag="OO172_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5511592.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8977..9675)
                     /gene="yeiL"
                     /locus_tag="OO172_001349"
     CDS             complement(8977..9675)
                     /gene="yeiL"
                     /locus_tag="OO172_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCR5511593.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            9806..10747
                     /gene="rihB"
                     /locus_tag="OO172_001350"
     CDS             9806..10747
                     /gene="rihB"
                     /locus_tag="OO172_001350"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5511594.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10847..12097
                     /locus_tag="OO172_001351"
     CDS             10847..12097
                     /locus_tag="OO172_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5511595.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLVLNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12153..13241)
                     /locus_tag="OO172_001352"
     CDS             complement(12153..13241)
                     /locus_tag="OO172_001352"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5511596.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLVDCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13244..14101)
                     /gene="nfo"
                     /locus_tag="OO172_001353"
     CDS             complement(13244..14101)
                     /gene="nfo"
                     /locus_tag="OO172_001353"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5511597.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14175..15224)
                     /locus_tag="OO172_001354"
     CDS             complement(14175..15224)
                     /locus_tag="OO172_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5511598.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15323..16204
                     /locus_tag="OO172_001355"
     CDS             15323..16204
                     /locus_tag="OO172_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5511599.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16410..17879
                     /gene="lysP"
                     /locus_tag="OO172_001356"
     CDS             16410..17879
                     /gene="lysP"
                     /locus_tag="OO172_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5511600.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18171..20162
                     /gene="cirA"
                     /locus_tag="OO172_001357"
                     /pseudo
     CDS             18171..20162
                     /gene="cirA"
                     /locus_tag="OO172_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20194..21030)
                     /gene="yeiG"
                     /locus_tag="OO172_001358"
     CDS             complement(20194..21030)
                     /gene="yeiG"
                     /locus_tag="OO172_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR5511601.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21288..21956
                     /gene="folE"
                     /locus_tag="OO172_001359"
     CDS             21288..21956
                     /gene="folE"
                     /locus_tag="OO172_001359"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5511602.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21973..23130
                     /locus_tag="OO172_001360"
     CDS             21973..23130
                     /locus_tag="OO172_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5511603.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23272..24311
                     /gene="galS"
                     /locus_tag="OO172_001361"
                     /pseudo
     CDS             23272..24311
                     /gene="galS"
                     /locus_tag="OO172_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            24591..25589
                     /gene="mglB"
                     /locus_tag="OO172_001362"
     CDS             24591..25589
                     /gene="mglB"
                     /locus_tag="OO172_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5511604.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25650..27170
                     /gene="mglA"
                     /locus_tag="OO172_001363"
     CDS             25650..27170
                     /gene="mglA"
                     /locus_tag="OO172_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5511605.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27186..28196
                     /gene="mglC"
                     /locus_tag="OO172_001364"
     CDS             27186..28196
                     /gene="mglC"
                     /locus_tag="OO172_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5511606.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28267..29502)
                     /gene="preA"
                     /locus_tag="OO172_001365"
     CDS             complement(28267..29502)
                     /gene="preA"
                     /locus_tag="OO172_001365"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR5511607.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29496..30736)
                     /gene="preT"
                     /locus_tag="OO172_001366"
                     /pseudo
     CDS             complement(29496..30736)
                     /gene="preT"
                     /locus_tag="OO172_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(30930..31169)
                     /locus_tag="OO172_001367"
     CDS             complement(30930..31169)
                     /locus_tag="OO172_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5511608.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31172..31891)
                     /gene="sanA"
                     /locus_tag="OO172_001368"
     CDS             complement(31172..31891)
                     /gene="sanA"
                     /locus_tag="OO172_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5511609.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32041..32925)
                     /gene="cdd"
                     /locus_tag="OO172_001369"
     CDS             complement(32041..32925)
                     /gene="cdd"
                     /locus_tag="OO172_001369"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5511610.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33055..33750)
                     /locus_tag="OO172_001370"
     CDS             complement(33055..33750)
                     /locus_tag="OO172_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5511611.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33747..34145)
                     /locus_tag="OO172_001371"
     CDS             complement(33747..34145)
                     /locus_tag="OO172_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5511612.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34384..>35267
                     /gene="dusC"
                     /locus_tag="OO172_001372"
     CDS             34384..>35267
                     /gene="dusC"
                     /locus_tag="OO172_001372"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5511613.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8656 a         8618 c         9317 g         8676 t
ORIGIN      
        1 gcaaaccaga acacagcgtg ataattccag atctcggcga cgatgcccgc cactgatcct
       61 gcgatgatcc agcccacgcg cgacgtgttg gtatagagcg tggtggctga acctgcctga
      121 ccgggcatca aatcctgaaa atagagcatc ccgatgccgc ccagaatgcc aataaaaata
      181 gcatttagca gctgcaagcc caacagaatg gcaggtgaat gcgccatcag cattcctgcg
      241 taaaaacaga cgccacccac ggcagcaacg cgcattaaga aacgcttacc cagacgtttg
      301 gcgaaatatc cggcaatcaa catcgtcggg atttccagcc cggcggcggt ccccatcatc
      361 acaccggcca gtttctcggg aagatgcagt tcgttgataa taaatagcgg catgttgatg
      421 atgtacaggc tgttcgagcc ccacatcaat gtacaaatga caaacagcag tagcgtatcg
      481 cgacggttac ggcgcggcgc ttcaaccgtg ccggtcgcca gcggaagctc tttttgcatc
      541 gacggtaaaa acagccacac catcacgccg caaacaataa acgctaccgc tgcgctcaga
      601 tacattaccg taaagctgaa acccatcgct aacgcataag ccagcggtgg gccaatgacc
      661 catgccagtg aaacctgagc gcgtaaaaaa gagctgaaca tcaccgcctc acgtccggtt
      721 ttgtcggcat gttcacgggc aagggcaaac atttgcgggt tagcggtcga gccaaagctg
      781 ctaagaaaga cgccaacgaa tagcaaaaca aagtagttgc gattccaggc aaaaagggtg
      841 caggccagca cgcctaacag gcagcaaaag acaatcagcg atttgcgatc gccgcgctta
      901 tcagagcgcc cagcgagaaa ctgacttacc agaatcccaa tgacagcgct gccggtaaag
      961 aagaatccca ccatcgccgg acgggcatgt atttcatcgg taaggaaaat actgagtgtc
     1021 ggggtttgca gagcacccgc aataccggtg agaaaggcaa cgattaaaaa cgccgtcgag
     1081 gtcaggtcaa acgatttcgc gctggagact gcgggggagt tatgcatgct ggtcgatcac
     1141 tggaagagga agacgcggag tttacgctgc ttgtcagaaa ataaacagac agcgaaatca
     1201 aaagcgccac gaataatcaa aatgccattt cactgttgct gattggctgc tgacgctgct
     1261 gaaatgaaat tgctgataac ggattttccc atcagcaatt aggaaaaatg acaaaaaatt
     1321 gtgcagcaca tcaaactttt gctcataact ttacggcttt ccttgcgtgc tgaaacagaa
     1381 gtattatgct ttcttgaaac gtttcagcgc gatcttgtct ttaaccctaa gccaggttgg
     1441 cgcttttttt ctcatagagg ctgaatcgtt tcaattcagc aagagaggag aactatgttc
     1501 cagttatccg tacaggacat ccatccgggc gaaaaggccg gagacaaaga agaggcgatt
     1561 cgccaggtcg ctgcggcgct ggtgcaggcc ggtaatgtag cagaaggcta cgtcaatggc
     1621 atgctggcgc gcgaacagca aacctcaacg ttcctcggca attgtattgc tattccacac
     1681 ggcactaccg acacccgcga tcaggtgctg aaaaccggcg ttcaggtatt tcagttcccg
     1741 gaaggcgtca cctggggtga cggtcaggta gcgtacgtgg caatcggtat tgctgccagc
     1801 tcggataaac atctgggcct gctacgccag ctgacccacg tactgagcga tgattccgtt
     1861 gctgaacaac tgaagtcagc aacaacagca gaagaacttc gcgcattact gatgggcgaa
     1921 aagcagagtg agcagctgaa gctcgacaac gaaatgctga cgctggatat cgtcgccagc
     1981 gatctgctga ctcttcaggc gctgaacgct gcgcgtctga aagaggcgag ggcagttgac
     2041 gccactttcg tcaccaaagc catcaatgaa caaccgctga atctcggaca gggtatctgg
     2101 ctgagcgata gcgccgaagg caatctgcgt agtgcgattg cggtaagccg tgcggcaaat
     2161 gcttttgatg tggacggcga aacggcagcc atgctggtga gtgtggcgat gaatgacgat
     2221 cagcccatcg cggttcttaa gcgtctcgct gatttgttgc tcgacaataa agctgaccgc
     2281 ttgctgaaag cggatgcggc aacgttgctg gcgctgctga ccagcgatga tgcgccgacc
     2341 gacgacgtgt taagcgcgga gtttgtggtg cgcaatgaac acggcctgca tgctcgtcca
     2401 ggtaccatgc tggtcaatac cattaaacaa tttaacagtg atattaccgt gacaaacctt
     2461 gatggcaccg gcaaaccggc aaacggacgt agtctgatga aagttgtggc acttggcgtt
     2521 aagaaaggtc atcgcctacg ctttaccgcc cagggtgcag atgctgaaca ggcgctgaaa
     2581 gccattggcg acgctatcgc tgctggtctt ggggagggcg cataatgagc agacgtgttg
     2641 ctacgatcac ccttaatccg gcttatgacc ttgttggttt ctgcccggaa attgaacgcg
     2701 gcgaagtgaa cctggtgaaa accaccggtc tgcatgcggc gggtaaaggc atcaacgtgg
     2761 ccaaagtatt aaaagacctg ggaattgatg tcaccgttgg cggcttcctg ggtaaagaca
     2821 atcaggatgg ttttcagcaa ctgttcagcg agctgggcat tgccaaccgt ttccaggttg
     2881 tacaggggcg cacccgaatt aacgttaagc tgacggaaaa agacggcgaa gtgaccgact
     2941 tcaacttctc gggttttgaa gtcacccccg ccgactggga acgctttgtg actgattctc
     3001 tgagctggct cggtcagttc gatatggtct gtgtcagcgg aagcttaccg tcaggcgtca
     3061 gcccggaagc gttcaccgac tggatgactc gcctgcgtag tcagtgtcct tgcattatct
     3121 ttgatagtag ccgtgaagcg ttagtagcag gtttgaaagc ggcaccgtgg ctggtgaaac
     3181 ctaaccgccg cgagttggaa atctgggcag gccgtaaact gcctgaaatg aaagatgtga
     3241 ttgaagctgc acatgcgcta cgtgaacaag gtattgcgca tgttgttatt tcactgggtg
     3301 ccgaaggcgc gctttgggtt aatgcctccg gcgaatggat cgctaaacca ccgtcagtcg
     3361 atgtcgtaag caccgttggc gcaggggatt ctatggttgg tggcctgatt tatggcttgc
     3421 tgatgcgtga atccagtgaa cacacactgc gtctggcgac agctgttgca gccctggcgg
     3481 taagtcaaag caatgtgggt attaccgatc gtccgcagtt ggccgcaatg atggcgcgcg
     3541 tcgacttaca accttttaac tgacagcagg agaggcataa tgaaaacgct gctgattatt
     3601 gacgctaatc tcggtcaggc acgcgcctat atggcgaaga ccctgctggg cgcggcggcg
     3661 cgaaaagcaa aactggaaat catcgacaat ccgaacgacg cagaaatggc gattgttctc
     3721 ggtgattcca tcccgaatga cagcgcgctg aacggtaaaa atgtctggct gggtgatatt
     3781 tcccgggcag ttgcgcaccc tgagctgttc ctgagtgaag ccaaaggcca tgcgaaacct
     3841 tacactgcgc cggtcactgc gacagcaccg gttgccgcca gcggtccgaa acgcgtagtt
     3901 gcggtgactg cttgcccgac tggcgtagca cacaccttta tggcggctga agccattgaa
     3961 accgaagcga aaaaacgtgg ctggtgggtg aaagttgaaa cccgtggttc tgttggcgcg
     4021 ggtaatgcaa tcactcccga ggaagtagcc gcagcggatc tggtgattgt ggcggcagat
     4081 atcgaagtgg atctggcgaa atttgctggt aaaccgatgt atcgtacctc taccggtctg
     4141 gcgctgaaga aaacctcgca ggaactggat aaagcggttg ctgaagcaac gccgtatgaa
     4201 ccggcgggca aagctcaaac ggcgaccact gaaggtaaga aagagagtgc aggcgcttat
     4261 cgtcacttgc taacgggcgt ctcttacatg ctgccgatgg tcgttgctgg tggtctgtgt
     4321 atcgcgcttt cttttgcttt tggtatcgaa gcgtttaaag agccgggtac gttggcagcg
     4381 gcgctgatgc agattggtgg tggttcagcc tttgcgctga tggtgccggt actggcaggt
     4441 tatattgcct tctccattgc cgatcgtccg ggcctcacgc cgggtctgat tggcggtatg
     4501 ctggcggtca gcaccggttc tggcttcatt ggcggtatta ttgcgggctt cctggctggt
     4561 tacattgcga agttaatcag tacgcaactg aaactgccgc agagtatgga ggcgctgaaa
     4621 ccgatcctga tcattccgct aatttccagt ctggtggtcg gtctggcgat gatctacctg
     4681 atcggtaaac cagttgctgg cattctcgaa gggctgactc actggctgca gaccatgggg
     4741 actgcgaatg cggttctgct gggggcgatc ctcggtggca tgatgtgtac tgacatgggc
     4801 ggtccggtaa acaaagcagc gtacgcattc ggtgtgggtc tgctgagtac tcaaacctat
     4861 ggcccgatgg cggcgattat ggcggcaggt atggtgccac cgctggcaat gggcctggca
     4921 acaatggtgg cgcgtcgcaa attcgacaaa gcgcagcagg aaggtggcaa agccgctctg
     4981 gtattggggc tgtgcttcat ttcggaaggt gcaattccgt ttgctgctcg tgatccgatg
     5041 cgtgtgctgc cgtgctgtat cgtgggtggg gcgctgactg gcgcaatctc aatggcgatt
     5101 ggtgcgaaac tgatggcacc gcacggtggt ctgtttgttc tgctgatccc tggcgctatt
     5161 acgccggtat tgggttacct ggtagcaatt attgccggta cgctggtggc gggtttggcc
     5221 tatgccttcc tgaaacgtcc ggaagtggac gcagtagcga aagcagcgta ataaaaggtg
     5281 tgttacaggg cagaaatttc ctgccctgta acatttgatg agcgcaaaga gaatgaccag
     5341 cttgtgaatg taattttatg aatttaaatg gttttatctt tcagtagaaa cttcaaacgt
     5401 tcatcaaaca ctaaggttat ttcctcaagg gcagattatt ttctgccctt ttttagtgtt
     5461 cctgttctgt gtatcccgtc acaaattcct cgcttttgta attcaggcat ttaatcgcat
     5521 tttgtgagcc tgctcaaatc tgcccatttt gcctctctgg cggtaattta ctttacgtaa
     5581 aacatgcata ctatgagcac atgtttaaat atgaacacat gtttaaaccg tctttgcagt
     5641 atctatattc ttgtggattg acagggttgg taatttttta ccagaggacg tgctatgcgc
     5701 gaaaaggatt atgtcgtaat tataggttcg gcgaatattg atgtcgccgg atattcacat
     5761 gaatcattaa attatgtgga ttcaaatcca ggtaaaataa aatttacgcc tggtggagta
     5821 gggcgcaata ttgcacaaaa cctggcgttg ctgggtaaca aagcctggct actgagcgcc
     5881 gtaggcagtg atttttatgg tcaatcgctg ctaacgcaaa ccaatcaatc tggtgtttat
     5941 gtcgataaat gcctgattgt gccgggagaa aatacgtcga gttatttatc attactcgat
     6001 aataccggtg aaatgctggt tgctataaat gacatgaata ttagcaacgc tattacagct
     6061 gaatatctcg cacagcaccg tgaatttatt cagagggcaa aggtcattgt cgcggactgt
     6121 aatatcagtg aagaggcact ggcatggatt ctggataatg ccgccaacgt acccgtattt
     6181 gtcgatccgg tttcagcgtc gaaatgtgtc aaagtgcgcg accgtctaaa tcagatccac
     6241 actctgaagc caaaccgcct tgaagcggaa accctgagtg ggattgcgct gtcagggcgt
     6301 gacgatgtgg caaaagttgc tgcctggttc catcaacatg gcctgaaccg actggtattg
     6361 agcatgggcg gcgacggcgt ttattacagc gatatcagcg gtgaaaatgg ctggtctgcg
     6421 ccgatcaaaa ccaatgttat taatgttacc ggagcgggcg atgccatgat ggcgggactt
     6481 gcttcgtgtt gggtagacgg aatgccgttt gccgaatctg ttcgtttcgc acagggatgt
     6541 tcgtcaatgg cgctctcctg tgaatacacc aataaccccg atttatcgat tgccaacgtt
     6601 atatcgttag tggagaacgc agaatgtctg aattaaaaat ttcccctgaa ttattacaaa
     6661 tttctccgga agtgcaggac gctttaaaaa acaaaaaacc ggttgtggcg ctggaatcaa
     6721 ccattatttc tcacgggatg ccgttcccac aaaatgccca gatcgcaatt gaagtagaag
     6781 aaactattcg taaacagggc gcagtacctg ccactatcgc cattattggc ggcgtgatga
     6841 aagtgggttt aagcaaagaa aaaattgaat tactgggtcg tgaagggcat aacgtgacca
     6901 aagttagtcg tcgcgattta ccttttgttg ttgccgccgg aaaaaatggc gcaaccactg
     6961 tggcttcaac gatgattatt gcggcgcttg ccggaattaa agtatttgcc accgggggaa
     7021 ttggtggtgt gcatcgcggg gcggaacata ccttcgatat ttctgccgat ttgcaagaac
     7081 tggcaaatac taatgtcacc gttgtttgtg ccggggcgaa atctattctc gatttaggat
     7141 taaccactga gtatttagaa accttcggtg tgccgttaat tggctatcag actaaagcgc
     7201 tgcctgcgtt tttctgccgt accagctcgt ttgacgtcag cattcgtctc gacagcgcca
     7261 gtgaaattgc ccgtgcaatg gcggtgaaat ggcaaagcgg gctgaacggt ggcctcgtgg
     7321 tagcgaaccc gatcccggaa cagtttgcga tgccggagga atctatcaat gcagccatag
     7381 atcaagccgt cgccgaagcc gaagagcagg gcgttattgg taaagaaagt acaccgttcc
     7441 tgctggcgcg cgttgctgag ctgaccggcg gtgacagcct gaaatccaat atccagctgg
     7501 tgttcaacaa cgccattctg gcgagtgaaa ttgccaaaga ataccagcgt ctcgcgggtt
     7561 aatttctgac gggcagaaag ccgtcgcccg tactcatcaa gcataccaaa acctggcgct
     7621 gacgccgggt ttactggcat gaagggaaaa tcattatgga tataatgaga agtgttgtgg
     7681 ggatggtggt gttactggca atagcatttc tgttgtcagt gaataaaaag agcatcagtt
     7741 tgcgcacggt tggagccgca ctgctgctgc aaatcgctat tggtggcatc atgctctact
     7801 tcccaccggg aaaatgggca gtagaacagg cggcattagg cgttcataaa gtgatgtctt
     7861 acagtgatgc cggtagcgcc ttcatttttg gttcgctggt tgggccgaaa atggatgtcc
     7921 tgtttgacgg tgcgggtttt atcttcgcct ttcgcgtact tccggcgatt attttcgtta
     7981 ctgcgctcat cagtctgctg tactacattg gcgtgatggg gctgctgatt cgcatccttg
     8041 gcagcatttt ccagaaagcc ctcaacatca gcaaaatcga atcttttgtt gcggttacca
     8101 ctattttcct cgggcaaaat gagatcccgg cgatcgttaa accgtttatc gatcgcgtga
     8161 atcgcaacga gttgtttacc gcaatttgta gcgggatggc gtccattgct ggttcgatga
     8221 tgattggtta tgccggaatg ggcgtaccaa ttgactacct gttagcggca tcgctgatgg
     8281 cgatccccgg cggtattttg tttgcacgta ttcttagccc ggcaaccgag ccttcgcagg
     8341 tcacatttga aaatctgtcg ttcagcgaaa cgccgccaaa aagctttatc gaagcggcgg
     8401 cgagcggtgc gatgaccggg ctgaaaatcg ccgctggtgt agcgacggtg gtaatggcgt
     8461 ttgtcgcaat tattgcgctg atcaacggca ttatcggcgg aattggcggc tggtttggtt
     8521 tcgccaatgc ctctctggaa agtatttttg gctatgtgct ggcaccgctg gcgtggatca
     8581 tgggtgtgga ctggagtgat gccaaccttg ctggtagcct gattgggcag aaactggcga
     8641 ttaacgaatt catcgcttac ctgagtttct ccccatacct gcaaacgggc ggcacgctgg
     8701 aagtgaaaac cgtggcgatt atatcctttg cgctttgtgg ttttgctaac tttggttcta
     8761 tcggtgttgt cgttggcgca ttttcggcta tttcgccaaa acgtgctcca gaaatcgccc
     8821 agcttggttt acgggcgctg gcagcagcaa cgctttccaa cctgatgagt gcgactattg
     8881 ccgggttctt tattggtctg gcgtaatgct cgatttgtag accggataaa acgtattaag
     8941 cgttgcatcc ggcaatcgcg ctattgcaaa taatttttac tgcatcatcc cggagaattt
     9001 attctccggg tccatctcca gcgccagtcc tgacaactgc tttctgtttt taattagata
     9061 tcctttcttg cttttaatta ataaaccgtc gtgaatgaat tgcgcgagaa catataaaag
     9121 atgtcgataa gaaacgccta aatactctgc cgcttgcgta tgcttttcgt gataaagatc
     9181 accttcttgt gagagtaata taaatgctgc caggcgattc attaacggaa atgattgatt
     9241 ctgagttaaa gaaacaatat tacgataatt tttatgactt aaggtgacgc aaagttttcg
     9301 taaaaatagg gtgtcgttta ataacagcgg acggtaatgt ttcataggga gcgcaaggca
     9361 ccaacactct tcaatagcct gcaccgcacg cggttcatgg tctttgtcga ttaactcaat
     9421 ctcgccaata aaacagggag cagcaaagaa atcgatcagc gacacgcgac cattagcaag
     9481 cgtggcgtaa agcctggcgc ggcctcgcgt cagataaaac agccatgacg gctgttgccc
     9541 ttcctgcaca atgtagtcac gcgctaaaaa atgaaacagc cgcgtatcgg ctgaaacatc
     9601 cgttgaaaag caatccttaa acgcagattc actcatcaat tgctgcttaa ggtcattatt
     9661 gtggatttct ttcacgatgt ttatccgcta tgagatttct catatttagc atacgtacgc
     9721 tgttgttaag attcaatcat cagcccggtc gaattttctt tgctgtagaa aaaaataacg
     9781 caactggaaa cagaggaaat aaacaatgga aaagagaaaa attattctgg attgtgagcc
     9841 gggtcatgat gatgctattg caatgatgat ggcagcgaaa catccggcaa tagatttatt
     9901 aggcatcact attgtagcgg gtaatcagac gcttgataaa acattaatta atggcctgaa
     9961 tgtttgccag aaactggaga ttaatgttcc ggtttatgcg gggatgccgc aacccattat
    10021 gcgtaaacaa atcgttgccg ataatattca cggtgaaacc ggactggatg gcccggtatt
    10081 cgagccgctg acccgccagg cagaaagtac ccatgcggtg aaatatatca tcgataccct
    10141 gatggcaagc gatggcgata tcactctggt gccggttggt ccgctttcaa atatcgcggt
    10201 ggcaatgcgt atgcaacccg caatcctgcc caaaatccgt gaaattgtgc taatgggcgg
    10261 cgcttacggt acaggcaact tcaccccatc tgccgagttc aatatctttg ccgacccgga
    10321 agccgcccgc gtggtgttca cctccggcgt tccattagtg atgatgggcc tcgatcttac
    10381 caaccagacc gtttgcaccc cggatgtaat tgctcggatg gaaagggtag gcggccctgc
    10441 cggagagctg ttcagcgaca tcatgaactt cactctcaaa acgcagttcg aatactacgg
    10501 cctggctggc ggcccggtgc atgacgccac ctgcattggt tatctgatca accctgatgg
    10561 cattaaaacc caggatatgt acgtcgaagt ggacgtcaac agcggccctt gctacgggcg
    10621 tacggtctgc gacgaactgg gcgttcttgg caagcccgct aataccaaag tcggcatcac
    10681 tattgatacc gactggttct ggggcttagt cgaagagtgc gtgcgcggct acatcaaaac
    10741 ccattaagtg ttgataacaa gccgggacag tatcgcaaga tgcaggcccg gctatttctg
    10801 atgggcagaa agccgtcgcc caaaatgaca tgaagagata atcactatgg atgtcatgag
    10861 aagtgttctg ggaatggtgg tactgctggc aattgcgttt ttactgtcag taaacaagaa
    10921 gaagatcagc ctgcgtaccg ttggcgcggc gttagtgtta caggtcgtga ttggcgggat
    10981 tatgctttgg ttaccgccag ggcgttgggt cgcagaaaaa gtcgcttttg gcgtccacaa
    11041 agtgatggcg tacagcgacg caggtagcgc atttatcttc ggttctctgg tcggaccgaa
    11101 aatggatacc ttatttgatg gcgcaggatt tatctttggt ttcagggtat taccggcaat
    11161 tatcttcgtc actgcactgg tgagtattct ctactacatc ggtgtgatgg ggatcttaat
    11221 tcgcattctc ggcggtatct tccagaaagc tttaaatatc agcaagatcg agtcattcgt
    11281 cgcggtcacc accattttcc tcgtgctaaa cgaaattccg gcgatcgtca aaccctttat
    11341 cgatcgtctg aatcgcaatg aattatttac agcgatttgt agtggcatgg cctcgattgc
    11401 tggttcgaca atgattggtt acgccgcact gggcgtacct gtggaatatt tgctggcggc
    11461 atcgttaatg gcgatccctg gcgggatctt gtttgcccgc ctgttaagcc cggctacgga
    11521 atcttcgcag gtttctttta ataacctctc tttcaccgaa acaccgccaa aaagcattat
    11581 tgaagccgat gcgacagggg caatgaccgg gctgaaaatc gccgccggtg tagcgacagt
    11641 tgttatggca tttgtcgcca tcattgcgtt aattaacggt attatcggcg gcgttggcgg
    11701 ctggtttggt tttgaacatg cctcgctgga gtccatttta ggttacctgc tggccccact
    11761 tgcgtgggta atgggtgttg actggagtga tgcaaatctt gccgggagtt tgattggaca
    11821 gaaactggca ataaatgaat ttgtcgctta tctcaatttc tcaccctatc tgcaaacggc
    11881 tggcactctg gatgctaaaa ccgtggcgat tatttccttc gcgttgtgcg gtttcgctaa
    11941 ctttggttct atcggggtgg tggtgggggc gttttctgcg gttgcgccac accgtgcgcc
    12001 ggaaatcgcc cagcttggtt tacgcgcgct ggcggcggcg acactttcta atctgatgag
    12061 tgccaccatt gccgggttct ttattggttt agcgtagtgt gcaatttatg agccggatta
    12121 gatgcggtca agcgtcgaat ccggcaacat tgttacacca ttggcactaa tgacagcgcg
    12181 ttatcggcag acagggtagg gttgtttaag ctgccgctgg cgcgtgaaat tgccgcgcag
    12241 gccatcgcaa aacgggcgct gtcacggaaa ttgtttccct ccagaaagct atataccaga
    12301 cccgccataa aaccatcgtc agcaccaaaa ctgtctaccg tcgtatgcgc tggcacagtc
    12361 agcaaaaatt gttcaccatc cttttcgctg caataaactg actcatcggg caaataaaca
    12421 aacagttgct gaacaccttg ctgatgcaat gcattcactg cggcattacg gtcagcatcg
    12481 ctggtgatcg cctgtcccca taaaatttcc agctccggta aagtgggttt cagggtgtga
    12541 atatgcgcca gccagtgttt gattttgccc gctttgaatt ctgaaacggt atcgacaaac
    12601 accgggatct catcagcgag ggtaaagacc cattccagcg cctcggctgt caggttgcaa
    12661 tcaaccagta ccacgcccgc atgacgaagt aaatcgcgcg acccgttcag tagttgcggt
    12721 gtcaactgtt ccagcagatg ggtatcgtta atcgccagca cggtttgatc gtctcgattg
    12781 gcaattgcca gatacgtcga tgtgctttga ccatgcaaac gaacgcagcc ggagacattc
    12841 acgccggcac ggcgcgtttc ttccaggagc atttcgccat aaaagtcatc gccaatcact
    12901 gaaagcaaat ggacgtcacg gcctaacagc gccagattgt gggcgatatt gcgtccaacg
    12961 ccgcctgccg aacaatgaat tgtaccggga tgagaagccg cttgcgggta atggatatcc
    13021 gccatcccgc gaatatccat attgattgtc cccaccacta cgcagtattc ctgctcggtg
    13081 agaatgtaac ctttgccttt aatccggcct ttgcgcatta aatccataat atgcgccgca
    13141 acgcgcgaac gactgatttg cagcatgtcc gcaatttcgt tctgctgaat cagcgggtta
    13201 cgccgtaaaa ttgcaaggat ctccttttcc cggttattca tcttcaggct accgcttttt
    13261 cagtttgttg cgctttcagc caggcgatct cttctgccca gatatccggg ttgatggttt
    13321 caaggatcag cggaatgccg tcgaaacggt cgttctgcat aatccagcgg aacgcatcat
    13381 gaccgatatt gccttcaccg aggctatgat ggcggtcaac gcggctgcca aaggtacttt
    13441 tcgcatcgtt aaggtgcatc ccgcgcagat acttaaagcc gacaatacgg gcaaaatcag
    13501 cgaacgtttt ttcgcattcg gctggagtac gcaaatcata cccggcagcg aaagcatggc
    13561 aggtatcaat gcagacgccg acgcgggatt tatcttccac gccgtcgata atcgccgcga
    13621 gatgttcgaa tttaaaccct aagttactgc cctgaccggc ggtgttttca atcaccgctg
    13681 ttacaccttg agttttatcc agcgcaatgt tgatggattc ggcaatacgc gcaaggcaat
    13741 cctcttctga aatctgcatc agatggctgc cagggtggaa gttgagcaaa gaaagcccca
    13801 gttgttcgca acgctgcatt tcatctataa aggcatcgcg cgatttttcc agagcttcag
    13861 tgaccggatg tccgaggttg atcagataac tgtcgtgggg aagaatttgc gccgatgtgt
    13921 agtgatattt ttcacaggcg gctttgaatt catcgatggt ttgcgtcgtg agcggtgcgg
    13981 cacgccactg acgttggttt ttggtgaaca aggcaaacgc ggttgcgtcg atttcggcgg
    14041 cgcgaattgc ggcatttgcc agaccgccag cagcactaac gtgcgctcca atgtatttca
    14101 tgcgaggact cctgttaaac ccgctggagg aaaacggtaa tgatagcggg ttaacaggag
    14161 caagaagtag tggtttatgc gatgacgctt tgaatcacat agttaatcgc accaccacca
    14221 acaatcagcc aggcaaacag taccagtgcc atcagcagag gtttcgcccc agcttttttc
    14281 agcgcgctga cgtgagtggt cagacccagc gccgccatcg ccattgccag caggaaggta
    14341 tccagcgtta ccagcatgtt caccacgctc tgcggtaaca ggtggaacga gttaaagatg
    14401 gcaactacga tgaacaagat ggcaaaccac ggaatagtga ttttgctttt ctcgccgctg
    14461 ttcgccccag acagctgttt aacacgcgcc gccagcagga tgaggaacgg agccagcatc
    14521 atcacgcgca gcattttgga aataactgct gcgttttctg catccgggct gatggcatga
    14581 cctgccgcca ccacctgcgc cacttcgtgc acagtagaac caatgtagat accgaaagtt
    14641 tccggactaa accattgaga catcagcgga tatatcgccg ggtagaggaa aatcgcgacg
    14701 gtcccgaaga taacaacggt tgcaacagcc acggttactt tactggcttc cgctttcact
    14761 accggctcag tcgccagtac cgcggcagca ccacagatac tgctaccggc accgatcaac
    14821 caactggtgt gcttatccag accaaacact ttctgcccca ggaagcaagc cagcaggaag
    14881 gtactggaca gcgtcagcac gtcaatgatg atcccactga taccgacatc ggcaatttgc
    14941 gagaacgtca gacggaagcc ataaagaatg atacccagac gtaataaata ttgcttggca
    15001 aacagcacac caccgtcaca gcttttccag atgtgcggat agatggtgtt gcctaaaacc
    15061 atccccaaca agattgcgag ggtgagggca ctaaacccgg cacccgcaac cgcgggaatg
    15121 gaaccacccc acagggcgac cccggtgata actgcactca gggctaaccc cggaataaaa
    15181 tgccacagtg tacgatgttg tttctgtaag gtgatattcg tcataaccct ctcctttaac
    15241 cgggcataag gttacgacta actggtttaa aaataaaatt gattatatat ttataattaa
    15301 tctttataag tggtaagcga ctatgcacat caccctccgg cagttggaag tttttgcaga
    15361 agtattgaaa agtggatcaa ccacccaggc gtcggtgatg ctggcgttgt cgcaatcagc
    15421 agtgagcgca gccttgactg acctggaagg gcagcttggc gttcaactgt ttgatcgcgt
    15481 ggggaaaaga ctggttgtta atgaacacgg gcggctgctc tatccgcgag cgttggcact
    15541 gcttgaacag gcggttgaaa tcgaacaact gtttcgcgaa gacaacggcg cgattcgtat
    15601 ctatgccagt agtaccatcg gtaactacat tctgcctgca gttatcgccc gttatcgcca
    15661 tgattatccg cagttgccga ttgaacttag cgttgggaac agccaggacg tgatgcaagc
    15721 ggtgctggat ttccgcgttg atattggctt tattgaagga ccgtgccaca gcactgaaat
    15781 catttctgaa ccgtggctgg aagacgagct ggtggttttc gccgcgccga cttcgccgtt
    15841 ggctcgtggt ccggtcacct tagaacagct ggccgctgcg ccgtggatcc tgcgtgaacg
    15901 cggttccggc acgcgggaga ttgtcgatta tctgttgctg tcacatttac cgaagtttga
    15961 gatggcgatg gaattaggta actccgaggc aatcaaacat gcggtgcgtc atgggttggg
    16021 aattagttgc ctgtcgcgac gtgtgattga agatcaattg caggcaggca cattaagtga
    16081 agttgcggtc cctctgccgc gcctgatgcg tacgttgtgg cgtatacatc atcggcaaaa
    16141 acacctttcc aacgcgctac ggcgctttct ggactattgc gatcccgcaa atgtgccgcg
    16201 ttaagttgct gtacaagaac atgctggtgc tgtgtcgatt tcgtgacgca gcgccttcag
    16261 catgcattcg ccagaaaaga gattggctgc tttacttata atccctgggc gatcatgaag
    16321 gtgtcttata accgtgtatt tctgccggaa ggattgccaa tcgtctgcta caatcgcgcc
    16381 atcattttta agatggatag catttttgta tgggttccga aactaaaact acagaagcgc
    16441 cgggcttacg ccgtgaatta aaggcgcgtc acctgacgat gattgccatt ggcggttcca
    16501 tcggtacagg tctttttgtt gcctctggcg caacgatttc tcaggcaggt ccgggcgggg
    16561 cattgctctc gtatatgctg attggcctga tggtttactt cctgatgacc agtctcggtg
    16621 aattggctgc atatatgccg gtttccggtt cgtttgccac ttacggtcag aactatgttg
    16681 aagaaggctt tggcttcgcg ctgggctgga actactggta caactgggcg gtgactattg
    16741 ccgttgacct ggttgcagca cagctggtca tgagctggtg gttcccggat acaccgggct
    16801 ggatctggag tgcgttgttc ctcggcgtta tcttcctgtt gaactacatc tctgttcgcg
    16861 gctttggtga agcggaatac tggttctcac tgatcaaagt cacgacagtt attgtcttta
    16921 tcatcgttgg cgtgctgatg attatcggta tcttcaaagg cgcgcagcct gcgggctgga
    16981 gcaactggac aatcggcgaa gcgccgtttg ctggtggttt tgcggcgatg atcggcgtag
    17041 ctatgattgt cggcttctct ttccagggaa ccgagctgat cggtattgct gcaggcgagt
    17101 ccgaagatcc ggcgaaaaac attccacgcg cggtacgtca ggtattctgg cgaatcctgt
    17161 tgttctatgt gttagcgatc ctgattatca gcctgattat tccgtatacc gatccgagcc
    17221 tgctgcgtaa cgatgttaaa gacatcagcg tcagtccgtt caccctggtg ttccagcacg
    17281 cgggtctgct ctctgcggcg gcggtgatga acgcagttat tctgacggcg gtgctgtcag
    17341 caggtaactc cggtatgtat gcgtctactc gtatgctgta caccctggcg tgtgacggta
    17401 aagcgccgcg cattttcgct aaattgtcgc gtggtggcgt gccgcgtaat gcgctgtatg
    17461 cgacgacggt gattgccggt ttgtgcttcc tgacctccat gtttggcaac cagacggtat
    17521 acctgtggct gctgaacacc tccgggatga cgggttttat cgcctggctg gggattgcca
    17581 ttagccacta tcgcttccgt cgcggttacg tattgcaggg acacgacatt aacgatctgc
    17641 cgtaccgttc aggtttcttc ccactggggc cgatcttcgc attcattctg tgtctgatta
    17701 tcactttggg ccagaactac gaagcgttcc tgaaagatac cattgactgg ggcggcgtag
    17761 cggcaacgta tattggtatc ccgctgttcc tgattatttg gttcggctac aagctgatta
    17821 aaggaactca cttcgtacgc tacagcgaaa tgaagttccc gcagaacgat aagaaataag
    17881 ttttctccct tccttgctaa gccctctcaa ccgagagggc tttttcaatt ccatttccct
    17941 gacaaatcat gcggatataa aatttaacat ttggattgat aattgttatc gtttgcatta
    18001 tcgttacgac gcaatcaaaa aaggctgaca aatcagaggc tgttccggct ttctgggatg
    18061 atcacctgca tacaaataag tccaccgcga tgctgccgta cgcaagggga cgtaaagaag
    18121 atgtgagcga tacccatttt attttcgtag ttacctcatg gagatatgga atgtttaggt
    18181 tgaacccttt cgtacgggtc gggctgtgtt tgtccgctat ttcttgtgca tggcctgtgt
    18241 tagcggtcga tgatgatggc gaaacgatgg ttgtcactgc atcttccgtg gaacaaaatc
    18301 ttaaagatgc acccgccagt atcagcgtca ttacccagga agacctgcag cgaaaaccgg
    18361 tacagaatct gaaggatgtc ctcaaagaag tgcctggcgt acaactgacg aacgaagggg
    18421 ataaccgtaa gggcgttagt attcgtggtc tggacagcag ctacaccctg attcttgtcg
    18481 acggtaaacg cgttaactcc cgcaatgccg tcttccgcca caatgatttc gatctgaact
    18541 ggatcccggt cgattccatc gaacgtattg aagtggttcg tggcccgatg tcgtcgctat
    18601 acggctccga tgcgctcggc ggtgtagtga atatcatcac caaaaaaatc ggtcagaaat
    18661 ggtcgggtac cgttaccgtc gataccacca ttcaggaaca tcgcgatcgc ggtgatacct
    18721 ataacggtca gttctttacc agtggaccat taattgatgg tgtgctggga atgaaagctt
    18781 acggcagcct ggcaaaacgt gaaaaggatg acccgcaaaa ctcaacgacc accgataccg
    18841 gagaaacgcc gcgtattgaa ggattctcca gccgcgacgg caatgtcgaa tttgcctgga
    18901 caccgaatca aaatcacgat tttactgccg gatacggttt cgaccgtcag gatcgtgatt
    18961 ccgactagct ggacaaaaac cgcctggaac gccagaacta ctgcgtcagc cataatgggc
    19021 gttgggatta cggcaccagc gaactgaaat cctacggtga gaaagtcgag aacaaaaacc
    19081 ctggcaacag cagcccgata acttccgaaa gcaatacggt cgacggcaaa tacacgttgc
    19141 cgctgacggc gattaatcag tttctcacgg ttggcggtga atggcgtcac gacaaactta
    19201 gcgatgcggt gaacctgacc gggggaacca gctccaaaac gtctgccagc cagtacgcgc
    19261 tgtttgtgga agatgaatgg cggatcttcg agccgctggc gctgacgacc ggcgtgcgta
    19321 tggacgatca cgaaacctac ggtgaacact ggagtccgcg tgcctacctg gtttataacg
    19381 ccaccgacac cgtaacggtg aaagggggct gggcgacggc atttaaagca ccttctctgt
    19441 tgcaacttag ccctgactgg acgagcaatt cctgccgtgg cgcatgtaag attgtgggta
    19501 gcccggatct gaaaccagaa accagcgaaa gttgggagct ggggctttac tacatgggtg
    19561 aagaaggctg gctggaaggg ggtgaatcca gcgttatcgt tttccgtaac gatgtgaaag
    19621 atcgtatcag catcagccgt acgtctgacg tcaacgctgc accgggctac caaaactttg
    19681 ttggttttga gacgggcgct aacggacggc gcataccggt atttagctac tacagcgtta
    19741 acaaagctcg tattcagggc gtggaaaccg aactgaaaat tccgttcaac gatgaatgga
    19801 aactgtcgat caactacacc tacaacgatg gtcgtgatgt cagcaacggc gaaaacaaac
    19861 cgctatccga tctgccgttc catactgcta acggtacgct ggactggaaa ccgctggcgc
    19921 tggaagactg gtcattctat gtttctggtc actataccgg gcagaaacgc gccgacagcg
    19981 cgacggctaa aacaccgggc ggttatacca tctggaatac cggcgcggcc tggcaggtga
    20041 ctaaagacgt caaactgcgc gcaggcgtgc tgaaccttgg cgacaaggat ctcagtcgtg
    20101 acgactacag ctataacgaa gacggacgtc gttactttat ggcagtggat tatcgcttct
    20161 gatgagaaga tgcccggcga accgggcgga ctttcacttc agtaaatact gcgcatggaa
    20221 gcgcaggtga tcctctataa aagaggcgat gaagtagtaa ctgtgatcat atcccggctg
    20281 aatacgcagc gtcatcggcc acgctttctg gcgcgcggct tctgccaata ccgcaggttg
    20341 caactggtcg gcaagaaact gatcattatc gccctgatcg ataagcgtcg ggatcgcatc
    20401 ctgcgcgtta ctggcataca tcagcgcgca actatcccat tccagccatg catttttgtc
    20461 ctcacctaaa taggtgctaa acgctttgat tccccacggg acgctgcacg gattcacaat
    20521 gggcgcaaag gccgaaacgc tggtgtattt acccggattt ttcagcgcca taatcagcgc
    20581 accgtgacca cccattgagt gaccgctaat ggcgcagcgg tcgctgacat taaattgcga
    20641 ctgaaccagc gccggtaatt catcgcgcag ataatcatac atccggtaat gcgtcgccca
    20701 cggcggttgc gtggcattaa gataaaagcc tgcgccctgg cctaaatcgt agccatcgtc
    20761 gttggcaacc ttttcgccgc gcgggctggt gtctggcatc accagtacaa tccccagttc
    20821 cgccgctacc cgctgggcac ccgccttggt ggtgaagttc tcgtcattgc aggttaatcc
    20881 ggaaagccag tacagcactg gtggcggggt gtgatcacga gggggtggga gaaagatact
    20941 gaacgtcatc gggcagttta aggtactgga gtcgtgtcgc cagcgttgct gccagccttc
    21001 aaaacagcgg tgctcttcga gcatttccat gcgtggctcc ttgttgtgtt gtttgcaatt
    21061 tcttcatcat accgtttatt caatgtgctg tgagtaactt tcacttccgt atttgcataa
    21121 cgatgtttta acatctgctg atgaaaggca gcggcaatta caataattat cgctgtgaat
    21181 actggattat gtgcgccgcc tcacgcacaa taatcaagct gtaaatcagc ttaataactt
    21241 tgcccccacg cagggcggag gcgtcacacc tgcaggagaa atcataaatg ccatcactca
    21301 gtaaagaagc ggccctggtt catgaagcgt tagttgcgag aggactggaa acaccgctgc
    21361 gcccgcccgt gcatgaaatg gataacgaaa cgcgcaaaag ccttattgct ggtcatatga
    21421 ccgaaatcat gcagctgctg aatctcgacc tggctgatga cagtttgatg gaaacgccgc
    21481 atcgcatcgc taaaatgtat gtcgatgaaa ttttctccgg tctggattac gccaatttcc
    21541 cgaaaatcac cctcattgaa aacaaaatga aggtcgatga aatggtcacc gtgcgcgata
    21601 tcactctgac cagtacctgt gaacaccatt ttgttaccat cgatggcaaa gcgacggtgg
    21661 cttatatccc gaaagattcg gtgatcggtc tgtcaaaaat taaccgtatc gtgcagttct
    21721 ttgcccagcg tccgcaggtg caggaacgtc tgacgcagca aattcttatt gcgctacaaa
    21781 cgctgctggg caccaataac gtggctgtct cgatcgacgc ggtgcattac tgcgtgaagg
    21841 cgcgtggcat ccgcgatgca accagtgcca cgacaacgac ctctcttggt ggattgttca
    21901 aatccagtca gaatacgcgc cacgagtttc tgcgcgctgt gcgccatcac aactgattaa
    21961 aaggcaggaa ccatggagcg caacgtcacg ctcgattttg ttcgcggcgt cgccattctg
    22021 gggatcctgc tattaaacat cagcgccttt gggctaccaa aggcggctta tctcaatccc
    22081 gcctggtacg atgctattac gccgcaggat gcatggacct gggcatttct cgatctcatc
    22141 ggccaggtga aattcctcac gctttttgcg ctgctgtttg gtgctggcct gcaaatgttg
    22201 ctgccccgtg gcagacgctg gatccagtcg cggttaacgc tgttagtctt gttgggcttt
    22261 attcacggtt tattgttctg ggacggcgat attctgctgg cttacgggct ggtgggctta
    22321 atctgctggt ggctggtgcg cgatgcgcca tcggtaaaaa gcctgtttaa taccggcgtc
    22381 atgctttatc tggtggggct tggcgttttg ctgttattgg ggctgatttc cgacagccag
    22441 accagccgcg cctggacgcc ggatgcatcg gctattttgt atgaaaaata ctggaagctt
    22501 cacggcggcg ttgaagcgat cagtaatcgt gccgatggtg ttggcaatag tttactggca
    22561 ctgggcgcac agtatggctg gcaactggca gggatgatgc tcattggtgc ggcgctgatg
    22621 cgcagcggct ggctgaaagg gcagttcagc ttacgtcact atcgtcgtac tggttttgtg
    22681 ctggtggcga ttggggtgac cattaacctt cctgccatcg ccctgcaatg gcagctggac
    22741 tgggcgtatc gctggtgtgc cttcttactt caaatgccgc gggaactgag tgcgccgttt
    22801 caggcgattg gctatgcgtc gctgttttat ggcttctggc cgcaattgag ccgctttaag
    22861 ctggtgcttg cgatcgcctg cgtcggacgg atggcgctga ccaactatct attgcaaacg
    22921 ctgatttgta ccacgctttt ttaccacctc ggtttgttta tgcattttga ccgcctggag
    22981 ctgctggcgt ttgttattcc ggtatggctg gcgaatatcc tcttctctgt tatctggctg
    23041 cgttacttcc gccaggggcc ggtggaatgg ctctggcgtc agttaacttt gcgtgctgcc
    23101 ggactggcaa tatctaaaac atcaagataa cgatctggat cacattcgtt aacaaaacgg
    23161 ctgtaaccgt ttccattgct gtgactcgat tcacgaagtc ctgtattcag tgctgacaaa
    23221 atagccgcca gcaagcagtc atttactgca atctcataac aggtagtgaa tatgatcacc
    23281 attcgtgatg tagcgcgtca ggctggcgtc tctgtggcaa cggtttcccg ggtgctcaat
    23341 aacagcacgc tggtcagtgc cgacacgcgt gaagcagtaa tgaaagccgt gagtgagctg
    23401 gattatcggc caaacgccaa tgcccaggcg ctggcaactc aggttagcga caccattggc
    23461 gtggtggtga tggacgtttc tgatgcgttt ttcggcgcgc tggtaaaagc ggtggatctg
    23521 gtcgcccagc agcatcagaa atacgtgcta atcggcaata gctatcatga agcggaaaaa
    23581 gagcgtcacg ccattgaggt gttaattcgc cagcgttgta atgcgttgat tgttcactca
    23641 aaagcattga gtgacgatga actggcacaa tttatggata acattcccgg tatggtgtta
    23701 atcaaccgcg ttgtgccggg gtacgcccat cgttgcgttt gcctggataa tctcagcggt
    23761 gcccgaatgg cgacgcgcat gttgctgaat aacggtcatc aacgtattgg ttatctttct
    23821 tccagccacg gcattgaaga tgacgccatg cgtaaagcag gctggatgag tgcgttgaaa
    23881 gagcaggata ttattccgcc ggaaagctgg attggcactg gtacgccgga catgccgggc
    23941 ggtgaggcgg cgatggttga actgctgggg cgcaatctac aacttaccgc tgtatttgct
    24001 tataacgaca atatggccgc tggcgcactg acagcattaa aagataatgg cattgcgatt
    24061 ccgttacatc tctcaatcat cggtttcgat gatattccca tcgcccgtta caccgacccg
    24121 caattaacga ccgtgcgtta tcccattgct tcaatggcta aattagccac cgaactggcc
    24181 ttgcaggggg cagcaggcaa tattgatcct cgtgccgcca ctgttttatg ccgacgttag
    24241 tgcgtcgcca ttctgtcgca acgcgccaga atgcggcggc gatcactaac tcaacaaatc
    24301 aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc
    24361 tatgattgca ccgttttaat gttgtaaccc gtatgtaaca gtgaataatc actttcaccg
    24421 aggtaatagc gtcataacaa caattaaagc cgttttctgg agcgttaccg ggcatggaag
    24481 aacgaatttt aaaaagtgag cttcggcgtt cagtaacact tcattaactc tactgccccg
    24541 ccgagcattt atctcaagca ctaccctgca taagaaaaac cggagatacc atgaataaga
    24601 aggtgttaac cctgtctgct gtgatggcca gcatgttatt cggtgccgct gcacacgctg
    24661 ctgatactcg cattggtgta acaatctata agtacgacga taactttatg tctgtagtgc
    24721 gcaaggctat tgagcaagat gcgaaagccg cgccagatgt tcagctgctg atgaatgatt
    24781 ctcagaatga ccagtccaag cagaacgatc agatcgacgt attgctggcg aaaggggtga
    24841 aggcactggc aatcaacctg gttgacccgg cagctgcggg tacggtgatt gagaaagcgc
    24901 gtgggcaaaa cgtgccggtg gttttcttca acaaagaacc gtctcgtaag gcgctggata
    24961 gctacgacaa agcctactac gttggcactg actccaaaga gtccggcatt attcagggcg
    25021 atttgattgc taaacactgg gcggcgaatc agggttggga tctgaacaaa gacggtcaga
    25081 ttcagttcgt actgctgaaa ggtgaaccgg gccatccgga tgcagaagca cgtaccactt
    25141 acgtgattaa agaattgaac gataaaggca tcaaaactga acagttacag ttagataccg
    25201 caatgtggga caccgctcag gcgaaagata agatggacgc ctggctgtct ggcccgaacg
    25261 ccaacaaaat cgaagtggtt atcgccaaca acgatgcgat ggcaatgggc gcggtagaag
    25321 cactgaaagc acacaacaag tccagcattc cggtgtttgg cgtcgatgcg ctgccagaag
    25381 cgctggcgct ggtgaaatcc ggtgcactgg cgggcacagt actgaacgat gctaacaacc
    25441 aggcgaaagc gacctttgat ctggcgaaaa acctggccga tggcaaaggt gcggctgatg
    25501 gcaccaactg gaaaatcgac aacaaagtgg tccgcgtacc ttatgttggc gtagataaag
    25561 acaacttggc tgaattcagc aagaaataag actgattggg cgcagtcgtt tactgcgccc
    25621 ttattgtatg gccaacaagg taaaattata tggtcagctc aacgactccg tcttccgggg
    25681 aatacttgtt ggaaatgagc ggtatcaaca agtcctttcc tggtgttaag gcacttgata
    25741 acgttaattt aaaagtccgg ccacattcta tccatgcatt aatgggggaa aacggtgcag
    25801 gaaaatcgac attattaaaa tgcctgtttg gtatttatca aaaagactcc ggcaccattt
    25861 tattccaggg taaagagatc gatttccatt ccgccaaaga ggcactggaa aatggtattt
    25921 cgatggtaca ccaggagtta aacctggtat tacaacgttc ggtgatggat aacatgtggc
    25981 tggggcgata tcccaccaaa ggcatgtttg tcgatcagga caaaatgtac cgcgaaacca
    26041 aagcgatttt tgatgaactg gatattgata tcgatccgcg tgcgcgcgtc ggcacattat
    26101 ccgtttcgca aatgcagatg atcgaaatcg ccaaagcgtt ttcctataac gcgaaaattg
    26161 tgattatgga tgaaccgact tcttcgttaa ccgaaaaaga ggtcaatcat ctgttcacta
    26221 ttattcgtaa attaaaagag cgcggctgcg gtattgttta tatctcgcat aaaatggaag
    26281 aaatcttcca gttatgtgat gaagttaccg tattgcgcga cggtcagtgg atcgccaccg
    26341 aaccgctggc aggactgacg atggacaaga tcatcggcat gatggttggg cgttctctta
    26401 atcagcgttt tcctgacaaa gaaaacaagc cgggcgaagt catcctcgag gtgcgtaacc
    26461 taacgtcact gcgccagccg tcgattcgcg atgtctcgtt tgatctgcat aaaggggaga
    26521 tcctcggtat tgccgggctg gtgggggcga aacgtaccga tattgttgag acgttatttg
    26581 gtattcgcga gaaatcggct ggcaccatta cgttgcacgg caaacagatc aataaccata
    26641 atgccaacga agccataaac cacggatttg cactggtaac tgaggagcgc cgctcaacgg
    26701 gaatttatgc ctatctggat attggtttta actcgttaat ttccaatatt cgcaactaca
    26761 aaaataaagt tggtttactg gataactcgc ggatgaaaag cgatacccag tgggtgattg
    26821 attcgatgcg ggtaaaaacg ccgggtcatc ggacgcaaat tggttcgctc tccggtggta
    26881 atcagcaaaa ggtgattatt ggtcgctggc tactaacgca accagaaata ttaatgctcg
    26941 atgaaccgac gcgcggtatt gatgtcgggg cgaagtttga aatttatcag ttaattgccg
    27001 aactggcgaa gaaaggcaag gggattatta ttatctcctc tgaaatgcct gagttgttag
    27061 ggataacaga ccgtattctg gtcatgagca atggtctcgt ttccggaatt gtcgatacaa
    27121 aaacaacaac gcaaaacgaa attctgcgtc ttgcgtcttt gcacctttaa gattaggggc
    27181 ttcccatgag tgcgttaaat aagaaaagtt ttcttactta cctgaaagag ggcggtattt
    27241 acgtcgttct tttagttttg ctggcgatta ttattttcca ggacccaaca tttttaagtc
    27301 tgttgaactt aagtaatatt ctcacccagt catcggtgcg tattattatc gcgctcggtg
    27361 tggcagggtt aattgtcacc caggggaccg atctttctgc tggtcgtcag gtagggctgg
    27421 cggcagtggt ggctgcgaca ttattgcagt ccatggataa cgccaacaaa gtgttcccgg
    27481 aaatggcgac gatgccgatt gcgctggtta ttctgattgt ctgtgccatt ggtgcggtga
    27541 tcggtttgat caacggtctg attatcgctt atctcaacgt gacgccgttc attaccacgc
    27601 tcggcacgat gatcatcgtc tatggcatca actcgctcta ttacgacttt gtcggggcgt
    27661 cgccaatttc tggttttgac agtggcttct ctacctttgc tcagggcttt gtcgcgctgg
    27721 ggagtttccg tctctcttac atcaccttct acgcgttgat tgcggtggcg ttcgtctggg
    27781 tgttgtggaa caaaacccgc ttcggtaaga acatttttgc cattggcggt aacccggaag
    27841 cggcaaaagt atctggtgtc aacgtcggcc tgaacctgct gatgatctac gcgttgtctg
    27901 gcgtgttcta tgcctttggc gggatgttag aagccggacg tatcggctct gccaccaaca
    27961 acctcggctt tatgtatgag ctggatgcta tcgcggcgtg cgtggtaggc ggtgtatcgt
    28021 tcagcggcgg tgtggggacg gtgattggcg tggtgaccgg ggtaattatt tttaccgtca
    28081 tcaactatgg cctgacgtat atcggcgtaa acccatactg gcagtacatt atcaaagggg
    28141 cgattattat cttcgccgta gcgctggatt cactgaaata cgcgcgtaag aagtgatgtg
    28201 attgcggata ttgccggatg cgctgcatcc ggcaatcggt gccaaatgcc ggatgcgtct
    28261 ctggttttac aacgttaccg ggtgttcttt ctcgcctttc ttaaacttca cttccccgag
    28321 ctcaatacaa cccaccgggc agacgtgacc acaaagcaga caacccacac atttctcggt
    28381 attacaatgc ggtgtgcggg ttttctcgct ccattccatc gcctggtgac cgccgtcgta
    28441 acaggaaata taacagcgtc cacagccaac acatttgtca agattgatac ggggatagac
    28501 aatataactg cggtctaaat cttccgccgg gacaatattg ttattcgcca gacctaccat
    28561 ctcctgcaac gaatcaaatc cctgatcggc gagataatgc gataacccgc tcgccatatc
    28621 ttctacgatg cgatagccgt actgcataat gccggtggtg acctgtaacg ttgctgcgcc
    28681 gagcaataaa aactccgccg catcttccca ggtttcaatg ccgccgatac cgctgattgg
    28741 gaaatcgcgc agttctggat gggtgcgcat ttgctgaatg aagcgcaggg cgatcggttt
    28801 taccgcttta ccggaatatc cggaaatact cgatttaccg ttaacgatcg gcataccgat
    28861 tttctgatta agatcgatat tggtgatgga tttaacggtg ttaattgccg caatgccatc
    28921 tgcgccgccg cgcttcgccg ccagcgccac ttcgcacata tcaccgatat tcggcgtcat
    28981 cttcgccagc attggcagcg tggaaccccg tttcactgcc cgacaatatt tttttaccag
    29041 ctccgggctt tgcccgacat cgctacccat cgcatgagaa gtcatttgcg gacaggagaa
    29101 gttacactcg atcatatccg cgccagcttc ttgcaccagg cgcgccagct cctcccattg
    29161 ctgctcattt tcccccatga tcgaagcgat caatactttg tccgggtaat cttccttcag
    29221 ccgacgcagg gcggtcagat tctcttccaa cggatgttca gcaatctgct ccatattttt
    29281 gaagccgata aaaccggtat cttctttcac cagatgatca aaacgcggcg agacttcgtt
    29341 ggcgataaaa aagccgatcg ttttaaacac cacaccgccc caacctgtgt cgtaggcttt
    29401 ggcacacatc tcatagcagt tgcctaccgg cgaagaagag aggcagaacg ggttgggaaa
    29461 gttcacgccg caaaaagtaa tcgaaagatc tttcgttaac atgagcaagc tccctctaat
    29521 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29581 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcgggtct gataattttg
    29641 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29701 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29761 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29821 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29881 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29941 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgg
    30001 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg gtcgctatgc tcaaacagcg
    30061 gtagcccgga accgcttgat aaccccacgg tgaccagtac cgcgcggttt tccgctttca
    30121 gttgctccag ggtgagtgtt ttaccgattt cgttattgca cttaatggtc acgcccattt
    30181 tttcaatacg ggcgatctct gcatccagca ctgattgcgg taaccggaat tgcggaatac
    30241 cgttacgcaa ccagccaccg gggtgcgctt ctttctcata aatcgtgacg tcataaccct
    30301 ggtttgtcag tgtcacactg gcctgcaatc cggcaggacc tgcgccaata atcgcgactt
    30361 tgccgagcgt tttagtaccg ggctgataaa tttccatccc ggtttgttgt tcaaaatcag
    30421 taacaaaacg ctgtaagcgg ccgatatcaa tgggagcatc aacaccggca cgggtacaac
    30481 cgctttggca taatttctcc gtcgggcaca ctctggcaca aacggcaccg agggcattat
    30541 tttcgcgaat tgtctcggca gcgcctttaa aattacgaaa gtagattgag cgaataaatt
    30601 tccccggatc ggtctgtgcc gggcaagcct gactacaggg agcgtcgtga cataataaac
    30661 agcgagaggc ttctttaatc gccagtaacg gcgtaaaagc cggagtgagt tcatccagat
    30721 aattttgttg cggcatgtga tcttccttaa tgctatgagt aattatggcg aacgctaaat
    30781 aaatattgcg tgctgatatt aaggctatgc gcgtggggaa atttaaactg tgaaaggatt
    30841 cacaaatagc tgcggttcag aatgttatat ccgcagctta ttgagtgaag taataattat
    30901 tctcaataat taacagaaaa gagaaaatat taacgataga aaataagata aacaatcatg
    30961 ccgatagata aaattccgaa gccaatatat gccaccatat aagcgaagaa gagtccggga
    31021 ttttttgctc gccagacata caccggttcc ggtgcatttt taacccgttt atcaatcgcg
    31081 cctgcgcgcc agcctttcca tgcttcgcgg aaacaaaata tcgggataag gaaaaaaacg
    31141 gcaacgacaa aaaactgctg tacatccata actactttcc ttgtttcttt tgtaattcaa
    31201 gtaactgttc gggtgtaacg gcgggatacc cctgcgcatc ttccggcacc tggtgcatag
    31261 ccggaatagg gaccagcggc cctaaaaaac gcggttcacg tttaaaaata taaaggtcag
    31321 ccagcgcacc gaaacgggcg gcaaattcgc gaatacgtac tgacagcata tctttcggtg
    31381 acggtacggc ataacactga gcctgaatcc ccatatgcag cgcaataaat aatgctcgct
    31441 cacagtggaa acgttgggtg ataataatga aatcattagt atcgaaaact ttgcgtgtac
    31501 gcacgatgga atccagcgta cgaaagcctg cgtaatcgag aacaatatct gatgggtcga
    31561 caccagcagc gattaaatct ttgcgcatgg tcatcggctc gttataactt tgcaatgcgt
    31621 tatcgccgct cagtaataga taatttacct taccgctgtt ataggcatta atcgctcctt
    31681 gaatgcggta gcgataatac tgattaataa cgccggtacg ataatatttt gctgtgccga
    31741 gcaccacgcc gacctggcgg taggggagat cctgcaattc gtcgtagata taaggcgcgg
    31801 ttttccagct catccagcga tcgaggccga gcacagtcaa cagcagcaag ccgatcagga
    31861 ctaacaggct gaggaacacg cgctttaaca tgcaggagac tcgagctaag gaaaatttcc
    31921 atcaggctac tttacccgct ggttttgcgc aagaaacggc aaactgtttg ccgggaattt
    31981 cgacaccagg agagggctgg caagccggac aaggcgttca cgccgcatcc ggcaccaggc
    32041 ttaagcgaga agcacgcggt cgatactgtg acagccgaga gctttcagcg ttgcggaggt
    32101 ggcatcccac tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc gctggatatc
    32161 cgggtaatca taacccttga gattcaacag aattaacgcc ccctgcaacg gcggcagagt
    32221 cgggttgaat gcggcgtttt cagcgtagct gccgctgaaa atacgaccgt ctttacattc
    32281 cagcgcgaca ccgcttggtg acttactgta aggcatgtgc gaacggtttg ccgctgcaat
    32341 cgccgcctga gaaagcgcat caccagtcag cgcatagccg tgatcctgct cgtccatcag
    32401 cagcgtttta atctccagat ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc
    32461 gtgtgcctcg cggcccggca aatgaatacg cagatccaga ccgctgttca gttcattcat
    32521 aaactgacgg cagtgaccac aaggcgtata gttaacggtg atggctgcaa gcgctttttc
    32581 accactcaac caggcgtggc tgatcgcgct ttgttcggca tgaacggttt gctgcattgt
    32641 tgcaccaata aattccatat tggcaccaaa ataccaggtt ccgctcacac cgcgcgcaat
    32701 tgcgccaaca ttaaaattcg acaatggcgt acgcgcacag gccgccgcca gcggaagtag
    32761 tgcgaatgcc agcgcgtctt cgtccagccc cgttgcgctc ttcagcgatg agacttgctc
    32821 cccggtcaac aaagcgggga agtacttgtc tgccagaata ggttccagtg cagattgcaa
    32881 gttatccgca agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa
    32941 tcatgtaatg ggataacagt ttacgaaccc gttatggctt tatatgtgat ctgaatctca
    33001 ttaattatgc aacacgtgaa acttacatca aaaatgcgcg acgtatcgca aattttagcc
    33061 cattactgcc agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca
    33121 taacaccagc gctagcgaac taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc
    33181 cgtaccgagg gcgtgcgagg cagtccccat tgccagaccg cgcgcagctt tggtacgaat
    33241 acgcatcgca ttaagcaatg tatggccaaa taccgcgccg aggatgccga cgaaaatcac
    33301 gcaaactgcg ctgattgccg gaataccgcc aatactgccg ccaaccgcca ttgcaatagg
    33361 cgtggtgact gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa
    33421 tgccacggaa gtccccgtca ccatcgcaac cacgctgccg ataaaacaga tggtgatgat
    33481 cgatttccag cgcgcgcgga tctggtgtag ctgctcatat aaaggatagg ccagcgcgac
    33541 gaccgccggt tgcagcagat cgtttagcac ttcgctacct ttaaagtaac tgtcgtaaga
    33601 gatgccggtc agcattaaaa aaggaatgat caccaccatt gctaccagca acgggttaag
    33661 caggggaaac ttataccgtg ccgccagttt gcgggcggca aaaaagacaa tcaacgtcag
    33721 cggtaatgac caccagatat tcgccatcat tcttctgatc ctttctgacc tacgacttta
    33781 cgttcaccgt gtaccagttg tgaactccag ctcaccacca gaaaaaccac cagcgtactg
    33841 acagcacagg aaaccactac cgggccaaac tgcgcgcgga gcaaatcaaa atattgcatg
    33901 acgcctacgc caatcggcac aaacaatagc gccatatagc gaatcagtac gtagcacccc
    33961 ggattgaccc atttcgccgg aagaatttgc aaggccagca ggacaaacag gatcagcatc
    34021 ccgatgatgc tgcccggaat ggttaccggt agcagggaag caatgaaaat gcctgcatac
    34081 aggcaggcat aaatcaggac gaaagcgcgt aaatattgcc agataatgtt cagtgtctta
    34141 ctcataatca acaccccaga tgagatggat tcatcataca attaattcgt aaaacgtgct
    34201 accgatcaca ttaagaaatc agcgcttatt ttttcgctac ccggtattac gcaatgagca
    34261 gcgaactcag gcatacatca agcatagaga gggattgcag tttgcagcaa tccctctttg
    34321 cagaccttta atccgctgac cataggcgct aacatagcgc ctcatttttt tgcgggtgat
    34381 gatatacgtg tgttactggc accgatggag ggagtgcttg actctctggt gcgtgaattg
    34441 ctgaccgaag ttaacgacta cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa
    34501 ctgctgccgg taaaagtctt tcatcgcatt tgccctgagc tacaaaacgc cagccggaca
    34561 ccatctggta cgctggtgcg cgtgcagttg ttaggtcagt tcccacaatg gctggcagag
    34621 aacgccgccc gtgcggtgga gttaggttcc tggggcgtgg atctcaattg cggctgcccg
    34681 tcgaaaacgg ttaacggtag cggcggcggg gcgacgttac tcaaagatcc tgaactcatc
    34741 taccaggggg caaaagcgat gcgtgaagct gtaccggcgc atttgcctgt cagcgtgaaa
    34801 gtgcgtctgg gctgggacag cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag
    34861 gctggcgcta cggagctggt ggtgcatggg cggacgaaag agcagggtta ccgcgcggag
    34921 catattgact ggcaggcgat tggcgagatt cgccagcggc tgaatattcc ggtgattgcc
    34981 aacggtgaaa tctgggactg gcagagcgcg caacaatgca tggcgatcag cggctgcgac
    35041 gcagtgatga ttggtcgcgg ggcgctcaat attcccaacc tgagccgggt ggtaaaatat
    35101 aacgaaccgc gaatgccgtg gccggaggtg gttgctttgc tgcaaaaata tacccgtctg
    35161 gaaaagcagg gcgataccgg gttatatcac gttgcgcgga ttaaacagtg gttgagttat
    35221 ttgcgtaaag aatacgatga agcaacggaa ttatttcagc atgttcg
//
LOCUS       DAJTNM010000025        35037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTNM010000025 DAJTNM010000000
VERSION     DAJTNM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.025"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..655)
                     /gene="rlmB"
                     /locus_tag="OO172_001373"
     CDS             complement(<1..655)
                     /gene="rlmB"
                     /locus_tag="OO172_001373"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5511614.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     "
     gene            complement(835..3276)
                     /gene="rnr"
                     /locus_tag="OO172_001374"
     CDS             complement(835..3276)
                     /gene="rnr"
                     /locus_tag="OO172_001374"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5511615.1"
                     /translation="MSQDPFQGREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3315..3740)
                     /gene="nsrR"
                     /locus_tag="OO172_001375"
     CDS             complement(3315..3740)
                     /gene="nsrR"
                     /locus_tag="OO172_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5511616.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(3945..5243)
                     /gene="purA"
                     /locus_tag="OO172_001376"
     CDS             complement(3945..5243)
                     /gene="purA"
                     /locus_tag="OO172_001376"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5511617.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5347..5544)
                     /locus_tag="OO172_001377"
     CDS             complement(5347..5544)
                     /locus_tag="OO172_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5511618.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5626..6630)
                     /gene="hflC"
                     /locus_tag="OO172_001378"
     CDS             complement(5626..6630)
                     /gene="hflC"
                     /locus_tag="OO172_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5511619.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6633..7892)
                     /gene="hflK"
                     /locus_tag="OO172_001379"
     CDS             complement(6633..7892)
                     /gene="hflK"
                     /locus_tag="OO172_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5511620.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7978..9258)
                     /gene="hflX"
                     /locus_tag="OO172_001380"
     CDS             complement(7978..9258)
                     /gene="hflX"
                     /locus_tag="OO172_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5511621.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9334..9642)
                     /gene="hfq"
                     /locus_tag="OO172_001381"
     CDS             complement(9334..9642)
                     /gene="hfq"
                     /locus_tag="OO172_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5511622.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9728..10678)
                     /gene="miaA"
                     /locus_tag="OO172_001382"
     CDS             complement(9728..10678)
                     /gene="miaA"
                     /locus_tag="OO172_001382"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5511623.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10671..12518)
                     /gene="mutL"
                     /locus_tag="OO172_001383"
     CDS             complement(10671..12518)
                     /gene="mutL"
                     /locus_tag="OO172_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5511624.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12528..13865)
                     /gene="amiB"
                     /locus_tag="OO172_001384"
     CDS             complement(12528..13865)
                     /gene="amiB"
                     /locus_tag="OO172_001384"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5511625.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13884..14345)
                     /gene="tsaE"
                     /locus_tag="OO172_001385"
     CDS             complement(13884..14345)
                     /gene="tsaE"
                     /locus_tag="OO172_001385"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5511626.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14317..15864)
                     /gene="nnr"
                     /locus_tag="OO172_001386"
     CDS             complement(14317..15864)
                     /gene="nnr"
                     /locus_tag="OO172_001386"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5511627.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15863..17002
                     /gene="queG"
                     /locus_tag="OO172_001387"
     CDS             15863..17002
                     /gene="queG"
                     /locus_tag="OO172_001387"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5511628.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16985..17038)
                     /locus_tag="OO172_001388"
     CDS             complement(16985..17038)
                     /locus_tag="OO172_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511629.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17271..17346)
                     /locus_tag="OO172_001389"
     tRNA            complement(17271..17346)
                     /locus_tag="OO172_001389"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17311..17313),aa:Gly,seq:gcc)
     gene            complement(17382..17457)
                     /locus_tag="OO172_001390"
     tRNA            complement(17382..17457)
                     /locus_tag="OO172_001390"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17422..17424),aa:Gly,seq:gcc)
     gene            complement(17615..17690)
                     /locus_tag="OO172_001391"
     tRNA            complement(17615..17690)
                     /locus_tag="OO172_001391"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17655..17657),aa:Gly,seq:gcc)
     gene            complement(17901..18446)
                     /gene="orn"
                     /locus_tag="OO172_001392"
     CDS             complement(17901..18446)
                     /gene="orn"
                     /locus_tag="OO172_001392"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5511630.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18541..19593
                     /gene="rsgA"
                     /locus_tag="OO172_001393"
     CDS             18541..19593
                     /gene="rsgA"
                     /locus_tag="OO172_001393"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5511631.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNIKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDG"
     gene            19690..20658
                     /gene="psd"
                     /locus_tag="OO172_001394"
     CDS             19690..20658
                     /gene="psd"
                     /locus_tag="OO172_001394"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5511632.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            20680..23991
                     /gene="mscM"
                     /locus_tag="OO172_001395"
     CDS             20680..23991
                     /gene="mscM"
                     /locus_tag="OO172_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5511633.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSIAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(24142..25644)
                     /gene="yjeM"
                     /locus_tag="OO172_001396"
     CDS             complement(24142..25644)
                     /gene="yjeM"
                     /locus_tag="OO172_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR5511634.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(25863..26840)
                     /gene="epmA"
                     /locus_tag="OO172_001397"
     CDS             complement(25863..26840)
                     /gene="epmA"
                     /locus_tag="OO172_001397"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5511635.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27165..28973
                     /gene="frdA"
                     /locus_tag="OO172_001398"
     CDS             27165..28973
                     /gene="frdA"
                     /locus_tag="OO172_001398"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5511636.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            28966..29700
                     /gene="frdB"
                     /locus_tag="OO172_001399"
     CDS             28966..29700
                     /gene="frdB"
                     /locus_tag="OO172_001399"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5511637.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29711..30106
                     /gene="frdC"
                     /locus_tag="OO172_001400"
     CDS             29711..30106
                     /gene="frdC"
                     /locus_tag="OO172_001400"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5511638.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30117..30476
                     /gene="frdD"
                     /locus_tag="OO172_001401"
     CDS             30117..30476
                     /gene="frdD"
                     /locus_tag="OO172_001401"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5511639.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            30539..31672
                     /locus_tag="OO172_001402"
     CDS             30539..31672
                     /locus_tag="OO172_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5511640.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            31761..32294
                     /gene="blc"
                     /locus_tag="OO172_001403"
     CDS             31761..32294
                     /gene="blc"
                     /locus_tag="OO172_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5511641.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32291..32520)
                     /locus_tag="OO172_001404"
                     /pseudo
     CDS             complement(32291..32520)
                     /locus_tag="OO172_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(32696..32842)
                     /gene="ecnB"
                     /locus_tag="OO172_001405"
     CDS             complement(32696..32842)
                     /gene="ecnB"
                     /locus_tag="OO172_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5511642.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(32953..33078)
                     /gene="ecnA"
                     /locus_tag="OO172_001406"
     CDS             complement(32953..33078)
                     /gene="ecnA"
                     /locus_tag="OO172_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5511643.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(33130..33696)
                     /gene="efp"
                     /locus_tag="OO172_001407"
     CDS             complement(33130..33696)
                     /gene="efp"
                     /locus_tag="OO172_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5511644.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            33738..34766
                     /gene="epmB"
                     /locus_tag="OO172_001408"
     CDS             33738..34766
                     /gene="epmB"
                     /locus_tag="OO172_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5511645.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8313 a         9597 c         8916 g         8211 t
ORIGIN      
        1 ccattgggat gctgatcaac tcatcgcaat gttcacgggt caggcgacgc ataccttcac
       61 cttccgcgcc catcaccagc gccaggcgtc cggtcatttt gctctgatag agtgtatgat
      121 ccgcctcgcc tgccgtaccg acgatccaga tattctcttc ctgcaacata cgcatggtgc
      181 gcgcaaggtt agtcacccga atcagcggaa cgctttctgc cgcgccgcag gctacttttt
      241 tcgccgtagc gttgagctgt gcggagcgat ctttcggcac aatcaccgca tgaacgcctg
      301 cggcgtccgc actacgcagg cacgcgccga ggttgtgcgg atcggttacg ccgtcgagga
      361 tcagcaggaa cggttgatcg agcgaagcga tcagatccgg cagatcgttt tcctgatact
      421 gacgtcctgg cttcacgcgg gcgataatgc cctgatgcac ggcaccgtcg cttttctcgt
      481 cgagatattg gcggtttgcc aactggataa ccacgccctg agactcaagg gcgtgaatca
      541 gcggtaacag acgtttatct tcacggcctt ttaaaataaa gacttcctga aaacgttcag
      601 gggcgcgctc cagcagggcc tgcactgcgt ggatgccgta aatcatttcg ctcattaatg
      661 tactcgttgt ttacgttttg cctgatgcac tacgtttatc cggcctacat gatctctgca
      721 atatattgaa tttgcgtgat tatgtaggcc ggataaggcg ttcacgccgc atccggcatg
      781 aacaaagcgc attttgtcag caatctaacc ctcttctttt aaagagggta ttgatcactc
      841 tgccactttt ttcttcgccg cacgcttcgc tttggtcgct gcggctattt tctgcgtttt
      901 cgccgatggc tttttcgctt ttctcgcgtc tttcttcgcc gctttcggct tcgttttttt
      961 ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt ttaccgacct gacgacgctt
     1021 gccgcctttt ttgcctgcat cgcctttttt cgctttctcg cgcgccgttt taccgacgtt
     1081 gcgcggtgcg cgttcactgg agatcagact aaagtcgatt ttgcgctcgt ccatattaac
     1141 cgcttcgacg cgaacttcca cgcgatcgcc caggcgataa gtctggccgc tggattcccc
     1201 catcaggcgt tgccctacct ggtcaaagcg atagtagtca ttgtccagcg aagagacatg
     1261 gaccagacca tcaatgaaca agtcgtccag acggacgaag aagccaaagc cagtgacgct
     1321 ggaaattacg cctttaaaga cgttacctac ctggtcgagc atgaagtcac acttcagcca
     1381 gtcagcgaca tcgcgcgttg cttcgtcggc acgacgttcc gccatcgaac agtgctgacc
     1441 cagttgcaac atctcttcca tcgaataatg gtagccgccg gtttcagtgg tgttgccctg
     1501 atgcccctgc tctttcgcca gcagatattt aatggcgcgg tgcagcgtca ggtcaggata
     1561 acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat gccagaccga agtgaccacg
     1621 gttttctgga tcgtaaatcg cctgtttcat cgagcgtagc agcatggttt gcagcatttc
     1681 tgcatcagga cggtcggcaa ccgactccag cagttccgcg tagtcacgcg gttccggctt
     1741 gttaccaccc ggcagctcca gccccagctc cgccagcact gaacggaaag aggtaatcgc
     1801 ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt tctttcgctt tctcaacgaa
     1861 acgcgccgcc gagatattcg ccagaatcat gcactcttca attaatttgt gcgcgtcgtt
     1921 acgctgggtc tgttcgatac gttcaatacg gcgttcagcg ttgaaaatga acttcgcttc
     1981 ttcgctctca aatgagatcc caccgcgttc ttcacgggct ttatccagca ctttatagag
     2041 gttatgcaac tcttcgagat gcttaaccag cggggcgtac tgctcgcgca gatcctgatc
     2101 gccctgcaga atatgccaga ctttggtgta ggtcagacgc gcgtgagagc tcatcaccgc
     2161 ttcgtagaat ttgtagcccg tcaggcggcc tttcgacgaa accgtcatct cgcacaccat
     2221 acacaggcgg tctacctgcg ggttgagcga acacaggccg ttagagagca cttccggcag
     2281 catcgggata acctgcgaag ggaagtacac cgacgtgcca cggttacgcg cttctctgtc
     2341 cagcggcgtt ggcggacgca catagtagct gacgtcggca atcgcgaccc ataaacgcca
     2401 gccgccgccg cgttttttct cgcagtaaac tgcatcgtca aagtcacggg cgtcttcgcc
     2461 atcaatggtg accagcggta aatcgcgcaa atcgacacgg cccgcttttg cttcttccgg
     2521 cacttcttct ttcagccccg caacctgttg ctcaacagcc tgcggccaga tatacggaat
     2581 ttcatgggta cgcagagcga tatcaaccgc catgccggtg cccatattgt cgcccagcac
     2641 ttcgacgatt ttacccaccg ctttggtgcg gcgagtcgga cgctgagtca gttcgactac
     2701 gaccacaaag cccatccgcg cgcccatgat ctgatcgggc gggattaaga tatcgaagct
     2761 cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct tcggtaaagt agcgaccaac
     2821 aatctggctg gtttttggca ccagtacgcg gacaatacgc gcttcacgac gacctttacg
     2881 gtcagcaccc agcggctgcg ccagcacctg atcgccatga atgcaggttt tcatctgctc
     2941 gctggagaga tacaaatcat ctttacgccc ttcaacccgc agaaagccgt agccatcacg
     3001 gtggccaata acggtacctt tcaccaggtc gaggcgttcc ggcagcgcat agcactgacg
     3061 acgagtgaag accagttgac catcgcgctc catcgcgcgc aggcggcgac gcaggccttc
     3121 aagctgctct tcgccttcaa tgtgcagttc taccgccagc tcatcacggc tggccggttt
     3181 ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta gggatgggat tcgcgtattt
     3241 ttcagcttcg cgtccctgga aaggatcttg tgacatctcg gttcctccgt tgtcatctct
     3301 gatgaagatt ttcgtcactc caacagcaat aatttataaa gcggttgatt ctcttcaacc
     3361 aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac tttgcacggc cttagaaagc
     3421 gcctgtttca accgacaggc aggtgtaatg tggcaaaact cactgctgca attcaccagc
     3481 gataagggct ccagctcgcg caccacatca ccaatacgta tcgcactcgc cggtttaccc
     3541 aggcgaatgc cgccattttt tccacgaaca gcagtcacgt agccggcacg actaagttga
     3601 ttgattattt tgaccatatg attacgggag acgccgtaga cgtcagtcac ttcagaaata
     3661 ctggtcatcc gcccttctgg caatgacgcc atgtagatca gcgcacgtaa tccgtaatca
     3721 gtgaaactcg ttaactgcac atcaacctca aaagggaaat cgggaaaaaa tacatttata
     3781 ttgatgataa accagccaca agctgtgtcg ctaatttatt tcagttcggg gaaggaaaaa
     3841 agcgaggatt taacactgtg tcggatagcg taatggctta accgacacag caatgtatca
     3901 cactgttagc ccggcaggca aaatatctgc caggcgtacc agaattacgc gtcgaacggg
     3961 tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag agatgatatc gatcggcaca
     4021 ccggtcagct cttcaatacg cttgatgtag ttcagcgccg cctgcggcag gccgctacga
     4081 tctttcacgc cgaaagtgga ttcagaccag cccggcatgg tttcgtaaat cggctctaca
     4141 cctttccagt cgtcagctgc cagcggagtg gtagtcactt cgcgaccatc cggcatacgg
     4201 taagccacgc agagtttaac ctctttcagg ccatccagaa cgtccagttt agtcaggcag
     4261 aagccagaca gggagttcag ctgtaccgca cgacgaacgg caacggtgtc cagccagccg
     4321 gtacgacgac gacgacccgt agttgcaccg aattcgttac cctgcttgca gaggaactcg
     4381 ccagtttcat caaacagttc ggccgggaaa ggacctgcac ctacacgagt tgagtaagct
     4441 ttgaggatac ccagaacgta atcaacataa cgcgggccca ggccggaacc ggtcgccacg
     4501 ccaccagcag tggtgttgga agaagttacg tacggataag taccgtggtc gatatccagc
     4561 agcgtaccct gcgcaccttc gaacatgacg aaatcgccac gctgacgcgc ctggtcgagc
     4621 aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg caacagccat cgtatcatcc
     4681 agaactttct ggtaatcaac cgcttcagct ttgtagtagt taaccaactg gaagttgtga
     4741 tattccatca cttctttcag tttttcagcg aaggtttctt tgtcgaaaag gtcgccaaca
     4801 cgcagaccgc gacgtgccac tttatcttca taagcaggcc cgataccacg accggtggtg
     4861 ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat ccagcgcaac gtgataatca
     4921 aggatcagcg gacatgcttc agacagcagc agacgctcac gaacggggat gccacggtct
     4981 tccagttctt tcatctcttt catcagcgcg gccggagaca gcacaacacc gttaccgatg
     5041 atgctggtta cattctcgcg gagaatacct gatggaataa gatggagaac ggttttttca
     5101 ccgttgatta cgagagtatg gcctgcgttg tgaccgccct ggtagcgtac aacatattta
     5161 gcccgttcag tcagaagatc gacgatctta cctttacctt cgtcacccca ttgggtgccc
     5221 agtacgacga cgttgttacc catttttcaa aatcaccgtt tgcttaaaaa tggattctac
     5281 catcgctttt tcagagttac agcacttttt gcactcaaaa atgacctcga acagtctgtt
     5341 ttttgttcag ccaatcgttt tcctcaacat gtagtagacc acaacgcccg caaccacaag
     5401 tccaccgcca aaacgacgta aaatattatc gggcaaattg gtcatcgcag agatcatctt
     5461 cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc agtaccaaaa ccagggcaag
     5521 cgccagccag attgtcgaat tcattcatta tccttataga aaaagaaaac caccgacatc
     5581 cctgaggatg cggtggcttt attgacctgt accgcagtcg ttatattaac gcgttgcgga
     5641 agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg ctcatgacca tcacgtcctg
     5701 attgccagag aagctgttct cataagcacg caggctacgg atgaatgcgt agaagtccgg
     5761 atctttactg aatgcatcag caaacagttt ggctgcttcg gcatcgcctt caccacgcat
     5821 aatgcggccc tgacgctcag cttctgccag cgttctggtc acttcatagt cggcagtcgc
     5881 gcgcagtttt tccgcttctt cctgaccttg tgaacggtga cgacgcgcta ccgcttcacg
     5941 ctcggcgcgc atacggttgt agatcgcttc agacacttcg gtcggcaggt tgatctgctt
     6001 gatacgcaca tcgacaactt caatacccag cgccgccata ctgttcgggt tgatgaccgg
     6061 aactttgccc ttcgtctcag ccgttacgcg ctctgccgct tcggcaatgg cgttatctgc
     6121 tgccggggta gtaacttcat cttctgtacc cgcagaaccg gagttcagcg cgtcacgtac
     6181 ttcgagggtc agacgaccac gggaatcggt gacgatatct ttcacgtcca ggcgaccaat
     6241 ttcagaacgc agacggtcag agaacttacg tttcaacagc acttccgctt gcgaaatgtc
     6301 gccaccaccc gttgccaggt agtaacggct gaaatcgctg atgcgccatt tgatgtaaga
     6361 gtcgacgatc aggtctttct tctctttggt cacaaagcgg tcggcctggt tgtccatggt
     6421 ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac ggtatcttga aatgcagacc
     6481 aggctcataa accaaaggtt tgttgtcatc gtcacgcagt accttaccaa aacgcagcgt
     6541 aataccacgc tcaccttctt tgacgacaaa gacagacatg taaagcacta ccagcacgat
     6601 gatgataatc gcgataactg acttacgcat cgttattccc cctgacgctg gtagtcgtta
     6661 cgctgcgcgt tggcgcggcg ttggtccata atatcgccct gactggtgga cgacgtgttg
     6721 cttgctccac tggttgtgga ggaagaggct ggcggcagac gcagcagatt gctggcaccg
     6781 ttatcgctct tcgccgcagg ggcgttacca cctttcagca tctggtctaa cggcagaacc
     6841 atcaggttgc cacctttatc gttaaccagc actttgcggg tgttacccaa cactttttcc
     6901 atcgtctcga tatacagacg ctcgcgagta atttccggcg cggctttata ttccggcaga
     6961 agtttagcaa agcgcgccac ttcaccctga gcttccagga tggtctgggc cttgtacgca
     7021 cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac gcggctgaac ttcgttggta
     7081 tacgcttctg cttcacgaat gtattgctgt tcgttttcac gcgcggcaat cgcatcgtca
     7141 aacgccgctt ttacttcttc cggcggacga gcagcctgga agttgacgtc cagcagcgtg
     7201 atacccatgt catacggacg aatcgtctct tccagttcgc gctgagtatc gctacgaatc
     7261 acggtacgac cttccgtcag aatgcggtcc atggtgtatt taccgataac tccacgcagg
     7321 gcgctgtcgg tagcctgacg caggctgtca tccgggctgg tcacgctata cagatatttt
     7381 tccggattgg tgacgcggta ctgcacgttc atctcaacgc gcactacgtt ctcgtccgac
     7441 gtcagcatca caccagaagc ggccagttca cgcacggctt ccacgttcac cggtttgact
     7501 tcgtcgataa acgtcggttt ccagttcaga cccggctcaa ccagatggct gaatttacca
     7561 aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat agaaaccact ggccgcccag
     7621 ataatgacaa tcgctgccgc tgcgatggta acgacacgac cgccaagctg cgggcgcggg
     7681 ccttgcgatg aactgccacc gccagatccg gtgcctttac cgccgcccag accaccgagc
     7741 tttttgctca gtttgcggaa gatatcatct aaatcaggtg gcccttgatc gcgaccgcct
     7801 ttgtttccat ttccctcaga gttgccgcca ggtttgctgc ttccccacgg gtcgcggtct
     7861 tgtccgttat taccgggctg attccacgcc atgtttgtgc tccatatttg ttatgcggtg
     7921 atccctgttg tctttcgacg ccagaggata tgactccacg cttcagacgc tacgccgtta
     7981 gatcaggtaa tcgatcaacg ccggttcttg tttacagagg cgacgccagt caacgatcgg
     8041 catacgaact tgcagactta cgctgccgtc ctcctccatc cactcttttt ctattgcctg
     8101 aagctgataa aaacgactcc tcagacgccc ttcctgcggt ggcagacgca atgtatgctg
     8161 cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa agctgtggta tccccgctcc
     8221 ggtctgtgcg gaaagccaga cacggatcgg tttgttctct tcgtcccgat caatacgcgg
     8281 ttcgaaatct tccagcatat cgatcttgtt catcaccagc agggttggga tctcgtgagc
     8341 gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt tcttgtacac gcacatccgc
     8401 cgcatcaatg acgtgcagca gtaatgtggc ttgccgcgtc tcttgtaacg tggctttaaa
     8461 tgctgccacc agatcgtgcg gcaggtggcg aataaaccct acggtatctg caagtacggt
     8521 ttcaccgaca tctgcaacgt caatacgccg caacgtcggg tcgagggtgg caaacaactg
     8581 gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa agggtagatt taccggcgtt
     8641 ggtatatccc accagcgaaa cagtaggaac gtcggctttg atacgcgatt gccgcccctg
     8701 ctcacgctgc ttttcaactc tttccaggcg cgactgtatc tgcacgatgc gattacgcaa
     8761 caaacgacgg tcggtttcga gctgggtttc acccggacca cgcaaaccta tcccgccttt
     8821 ctgtctttca aggtgggtcc agccacgcac caggcgcgta gccagatggc gcagctgcgc
     8881 cagctcaacc tgcaacttac cctcatgggt acgcgcacgt tgggcgaaaa tatctaaaat
     8941 aaggccggtg cggtcgataa cacgacactc gcacaaacgc tccaggttac gctcttgcgc
     9001 cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc gtcgctttga cagcttccgc
     9061 aatttcaact gctttacctt cacctacaaa atactttggg tgcggcgctt tacggctacc
     9121 ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc agagattcaa actcctggag
     9181 gtcttccata tctttgtctt gcgtaaaata gatgtgtacc agtaccgcct gctcaccagc
     9241 atcataacgg tcaaacaagc gtataaccct ctaaatagat cagcggggaa cgcaggatcg
     9301 ctggctcccc gtgtaaaaaa acagcccgaa accttattcg gtttcttcgc tgtcctgttg
     9361 cgcggaagta ttctgtgcgc tgctaccatg atggtagtta ctgctggtac cgccaccggc
     9421 gttgttactg tgatgagaaa ccgggcgaga tgggacaaca gtagaaatcg cgtgcttgta
     9481 aaccatctgg ctgaccgtgt ttttcaacag gatcacgaac tgatcaaaag actcgatttg
     9541 cccttgcagc ttaataccat tcaccaaata aatagaaact ggaacacgtt cccgacgcag
     9601 tgcgttcagg aacggatctt gtaaagattg ccccttagcc attctctctt ttccttatat
     9661 gcttatttgt actttgaacc tttcgattct gaaaaaattg cgcacgatac gtctcaattg
     9721 tacacattca gcctgcgata gcaccaacaa cctgtaatac ttcgtcacgc gcctgttctg
     9781 gtttttcact gtcaagccag tgaacccctt cccaaccacg cagccaggtt atctgccgct
     9841 tcgccaactg tctcgtggcg caaacacctc gataaaccat ttcatcgtat gagatttcgc
     9901 cttcaaggta agaccacatc tggcgataac ccacgcaacg aatggaaggc aagtccgtat
     9961 gcaaatctcc tcgggcaaaa agcgcccgga cttctgcttc aaaacctgaa gccaacatct
    10021 gatgaaaacg ctgctcaatg cgttgatgga gcagttcacg gctcgccggg gcgatggcga
    10081 actgatgcac ctgatacggt agagcgtctc ctgacgtttg cgtcagttcc gttaaagttt
    10141 tacccgaaat aaaaaaaact tccagtgccc gggaaagcct ttgtggatca tttggatgaa
    10201 tccttgctgc cgcaaccgga tctacctcct gaagttgacg atgcaatgac tcccaacctt
    10261 gctctgccgc ctgttgctca attctggccc gcacttccgg gtctgccgac ggtagcggcg
    10321 acaacccttc cagcaatgcc ttgaaataca acatcgtacc gcccactaac agtgggatcc
    10381 gccccgccgc ggtgatatcg gccatttccg ccagcgcatc gcggcgaaaa tcagcagccg
    10441 agtaagcctg cgacggatcg cgaatatcca gcaatcggtg cggcgcggcg agtaactctt
    10501 cagcgttcgg cttcgccgtc ccgatatcca tccctttgta aataagggca gaatcaacgc
    10561 ttatcaactc tactggtaaa attttacgca gctcaatggc taacgccgtt ttaccggagg
    10621 ccgtcggccc catcaaaaaa atcgccttag gcaggctcgc cttactgata tcactcatct
    10681 ttcagggctt ttatcgccgg atgtaaatca acagattgta acagaccacc cggcggcgtt
    10741 ttcacaagtt gcggacataa ccgttccacg tccgccagca gggttatggc ctgtgccatt
    10801 gaccactgcg catgttcgct catcagattt cgtgcaatcc actgcgcaat attgccaggt
    10861 tcgaatacgg actgcttcgc caggtagcct atcagttcag gaatcaagat ttgtaaattt
    10921 tgttggcgta agggtaaagg cacggccctg atggtcacat gctgtgcatc tgactggaaa
    10981 tcaataccca attccgctag ggcagactgc gctttttcta atgccgattt ttcttcgcca
    11041 gaaactttta gccgcaacgg aatcagcagc ggctgggcgc aaacgggcgc ttcacccggc
    11101 gtcaattgtg cctgacgcag ccaacgttct gccactggca aggataaaag tgaaatgttg
    11161 ccatcgcgct ccagcaacgc acagtcggaa tggacgatag tcagtacccg accaaaactc
    11221 tgactgttcg ccgcaagtgc aggttcctgc ggttccggcg cttttggttt ttgcatcggc
    11281 gcgggcgttt gcaaaagctg gcgatacact tcaccttgct gtttctggta gcctggctgc
    11341 gcattcggcc agggggcagc cggacgactg cctgaggcgg gcgcaggaga gtagcgcgga
    11401 gctaccggct cacgagctgc cggttctgca aagtgattgc gccccgccgc cacgcggttt
    11461 tccgggatgg cacgcggtgc gggttgtggt tcatcgtcca gcggtagcgg cgtttccagt
    11521 tgctgttgta gcacgctcag cacgccctga tagataaaat catgcaccag acgcgactga
    11581 tggaaacgca cttcgtgttt ggcggggtgt acgttgacgt ccacctgatg cgggtcgatc
    11641 tccagataca acacaaatgc cggttgctga tcggccccca gtttgtcttc gcaggcctgg
    11701 cggatcgcat gattgatcag gcgatcgcgc atcatgcgac cattcacgta gcaatactga
    11761 atttccgcca gtgcgggcgt agtgtgattt ggatcggcca cccagccgcg cagggtgaga
    11821 tcgccgtgtt gccattcaat cgccagcgct tgttcgagaa aagcggtgcc gcaaatcgcg
    11881 cctaagcgcc gttctttttg cccgccttcc ggcactgcgc ggtactgacg cacaatttta
    11941 ccgttatgcg acaggttgat cgtgacgtcg aaacgcgcca gcgcaatgcg gcggatgatt
    12001 tcatcaatat ggctaaattc ggttttctcg gtgcgcagga atttgcgccg cgccggggtg
    12061 ttgtagaaca gatccagcac ctccagcgtc gttcccaccg gatgcgccgc cggtttaacc
    12121 gtcacgtcca tgtcgcgtcc ttcggcatag gcctgccagg cttcctgctg ttctgcggtg
    12181 cgtgaagtga gcgtcaggcg ggaaaccgaa ctgatactcg ccagcgcctc accgcgaaag
    12241 cccaggctga taatggcttc gagatcgtcc agagaggcga ttttactggt ggcatgacga
    12301 gccagcgcca gcgccagctc atcttttttg ataccgcagc cgttatcacg aatgcggata
    12361 agtttcgccc caccgcgttc gatatcaata tcgatacgcg tcgcacctgc atcgaggctg
    12421 ttttccacta gttctttgac taccgacgca ggtcgctcga ccacctcacc tgcggcaatc
    12481 tggttcgcca gttgtggcgg taagacctga attggcatca atcgtcctta gtttggcagc
    12541 gtgcgatctg gcgtcgtcac cgtactggca gtttgtgccg ttgcaccctg cggcgcagat
    12601 tgcatcggat gcgcaaggaa gtaattgcgc aggcctttgt aaatggcttc tgccagctgt
    12661 tgttggtaat cgtcgctcgc cagcaaacgt tcttcgctgt tgttgctgat aaaaccggtt
    12721 tcgaccagta ctgatgggat atccggcgag cgcagaacgc caaggctggc gtgttctggt
    12781 cgacgtttat gtatttcgcc aatgcgttgc aactgactga tcatactggt cgctacatca
    12841 taccctaccc gctgggaatg accgaactgt aaatccagca ccgcctggct taaatagggg
    12901 tcagactgac tgttcgccag cacatcaccc gccccaccca gcagctccga ctgtttctcg
    12961 tgctgctcca gccagctggc catttcactg ttggcgcgac ggttagagag caccaatacg
    13021 gaagcgccag tcgcactgcg gttcggtgcg gcatcagcgt gaatcgacac gaggaaattg
    13081 gcgttttgct tatgtgccac atcactgcgc cccatcaccg agataaagta atccccgtca
    13141 cgggttaaaa cgcctttaaa catcggatcg tcattgagca aagtacgcaa tttacgcgcg
    13201 atggcgatgg tgacattttt ctcccgcgta ccaccggggc cgatagcgcc agggtcctga
    13261 ccgccgtgtc cggcatcaat agcgatgata attttatcgc cagtgttagc cgtcgcgcgc
    13321 gctgccggac gcgttaccgt attactgctg ataacacccg tagtgcggtt actttccgtt
    13381 ttaaacggat tgcgcgccgg ttcgctgacg cgcggtgcga caaccgcagg cgtttcaacg
    13441 cgtttcgcaa ccacgggcgg cggaggaggc ggtggcggca catcggcgtt aatcgtaaag
    13501 acgacagtgt aattgctgcc attctgccgc ttcaccgctt cggttttacc gttttcggta
    13561 agatcgacca ccagccgtag cgtttgtgca tctttaggcg ttccagagcg aatcgccttc
    13621 accagattat tgccgctgaa caacaacggc agtccctgaa tcacgcccgt ttgtttgata
    13681 tcgagcgcca cggtgcgttt gctttgatgg ctaaacgcat aatcaggatc gccaataaaa
    13741 ctcaacgtta tccgtgcctg ttgattaccg ttagaaacct gaatatcaga gagcgtcgcg
    13801 gcacccaccg gcgtgcacag cagcagcagc gtcgctacca accaatttct gatgcgatac
    13861 atcatcccgc cacctttcaa aggttaaccg gctaaacgcg ccagcaacaa ttcacccgca
    13921 gaggaaaccg cactcacgcg cgcctcacgg ccttgtgcct gataatcaat gtgtatttcg
    13981 acatccgggt caggaagaac acctgtacct tgttgtggcc actccacaag gcagatggca
    14041 tcgttggcaa aataatcgcg gatccccata aactccagct cctcgggatc ggcaaggcgg
    14101 tacaaatcaa agtgatagac cattaagttg tcgagcgtat agggttcgac cagcgtataa
    14161 gtggggcttt tgacattacc ctgatgaccc agagcctgta aaaagccccg gctaaaggtg
    14221 gttttacctg cgcctaaatc gccatacaga tagattacgg ttgctccatc gcaggctttc
    14281 gctacccgct cgcccaggtc taatgttgcc tgctcatcag ggagcggaat tactcgattc
    14341 atcatggttt ttatcagtca cttccgggtt aacaatacgc tgtagcgtgg aaaagagatc
    14401 ggttgccagc atcccgcgcg ttccaaaacg cgccgccagt acgtcagctg ccgcaccgtg
    14461 cgcgacacag cctgcacagg ctgcatcata cggcgacagt ttttgcccaa gcaatgcgcc
    14521 aataatacca gagagcacat cgcccatgcc gccgctcgcc atgcctgcat ttccggcatc
    14581 aataatgcct aaagcgtcag gatgggcggc gaccacggtt ccggcacctt tcagcaccgc
    14641 tacgccgcca taacgttgta ccagacgttt ggcgcaatgt aagcggtcac tttcaatttc
    14701 agcgacggaa cagcctaaca accgtgcggc ctcgctagga tgcggcgtga tcacgcgatt
    14761 gtgacgctta tcgggattga ttgccagcag gttcaatgca tcggcatccc acaacatcgg
    14821 tttgcgaaaa ttctcaactt tttgcagggc ttttttcccc cactcttgct ggcccagacc
    14881 gggaccaatc accaccacat cggcccattc caggctttcg gtaagagagt ccatagtcag
    14941 ttcatgcacc atcaattctg gtcgtgcagt cagcagcggc gcaatgttct cactgcgggt
    15001 cagtactcgg actaaaccag caccagcacg tagcgccgct tcccccgtca tacgaatagc
    15061 ccccgccgtg ccgtgatcgc caccgataat caccagccgc ccgtgatcgc ctttatgcga
    15121 agtcgggcga cgcggtttca gccagtgaga aagttgttct gccgaaaacc gctgaatttt
    15181 cgtctcctga cctgccagcc agctatccag ccccagtgag tcaaaatgca gttgtcctgt
    15241 aacatcccgc gcttttccag tgagcaagcc tagtttcagc gcaataaaag tgatggtgtg
    15301 atcggcgttg atcactgcgc ctggcgtagc gccggtttca gccagcaggc cggaagggat
    15361 atcaaccgcc acaatcggcg caggatggga attagcgtgg tcgattaact ggctaatgga
    15421 ttcgcggggc gcttgctgca agccggtacc gagcagcgca tcaacaatca gatctaccga
    15481 ttcgggccag acaatattcg aagcatggat ctcgccaccc gcgtttaacc atgcttcgcg
    15541 tgccagcgcg gcctcttccg gcaacggttt gtcgctctcc tgggccaaca acgtgacctc
    15601 aatgccgacc gctttggcca gtcgcgcgac cacgtagcca tcgccgccgt tattaccatg
    15661 accgcacagc accagccagt ggcgggcgtc aggatacgcc gaacgacaca cctggaatgc
    15721 ggcctcgcca gcgcgaagca tcagctcata gagtgtgagc cccagcacat ctgccgcctc
    15781 gcgttctccg cggcggatat cgtcggcgta ccagacggtg tgtggtatac ttacggggtt
    15841 tttcttcatt gtatggtccg tcatgtcaga gcccctcgat ctcaatcagt tagcgcaaaa
    15901 aattaaacag tgggggctgg aactgggctt tcagcaggta ggtattaccg ataccgatct
    15961 cagcgagtcc gagcccaaac tgcaagcatg gctggacaaa caataccacg gcgaaatgga
    16021 ctggatggca cgtcacggta tgctgcgcgc tcgcccccat gagttattac ccggtacgct
    16081 gcgtgtaatc agcgtgcgga tgaattacct tcctgctaac gccgcatttg ccagcacgct
    16141 gaaaaacccc aaactcggct atgttagccg ttatgcgctg ggccgtgact atcacaaact
    16201 tctgcgcaac cgactcaaaa agctgggcga gatgattcag caacattgtg tttcgctgaa
    16261 ttttagaccg tttgtcgatt ctgcgcctat tctcgagcgc ccgttagctg aaaaagctgg
    16321 gctcggctgg acaggtaagc actcacttat cctcaatcgc gaggccggtt cgttcttctt
    16381 tttaggcgaa ttgctggtcg atattccgct gcccgtggat caaccagtcg aggaaggatg
    16441 cggcaaatgc gtggcctgta tgacgatttg cccgaccggt gccatcgtcg agccatatac
    16501 cgtcgatgct cgccgctgta tctcttatct caccatcgaa cttgaagggg cgatcccgga
    16561 agagttgcga ccgttaatgg gaaaccgtat ttacggttgc gatgactgcc agcttatctg
    16621 cccgtggaat cgctattcgc aactcactac agaagacgat ttcagcccgc gtaagccgct
    16681 acacgcaccg gaactcattg agttatttgc ctggagcgaa gagaagtttt taaaagtcac
    16741 ggaaggatcg gcgattcgcc gtataggtca cctgcgctgg ctgcgtaata tcgccgtggc
    16801 attaggcaat gccccttggg atgaaacgat tttgacagcg ctggaaagtc gtaaaggtga
    16861 gcacccactt ctcgatgagc acatagcgtg ggcgattgcg cagcaaatag agagacgaaa
    16921 tgcgtgcata gtcgaagtgc aattgccgaa aaaacagcgt ctggttcggg tgattgaaaa
    16981 agggttaccg cgtgacgcct gattcatcca cagcttgtga ataaaaataa aaacgcattg
    17041 caattcaaga cgaataaaat cgtcaagtga tcgactcgac aaaacggaat cttgattttt
    17101 aacttattta tcaataggtt aaaaaatacc attcaccgtg cgaagtttct ttgaaattaa
    17161 gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag acaacaaaaa
    17221 ccagcaataa cgcatcgcgt cgctgtggat attttattga gagaagaatt tggagcggga
    17281 aacgagactc gaactcgcga ccccgacctt ggcaaggtcg tgctctacca actgagctat
    17341 tcccgcttgg gtggtctgtg ccttacagca ctttcaaatt ttggagcggg aaacgagact
    17401 cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta ttcccgctta
    17461 atcttcgtct ttcgaaccgc tactgccttg gctgccttca ctcaccttag tcacatactt
    17521 ttgtaagttc ccaaggattc gttcagttgc cgccttgtcg caattcgaaa tactgcgatt
    17581 gggtggtctg tgctttgcag cacttttcaa attttggagc gggaaacgag actcgaactc
    17641 gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc atactactta
    17701 attttgcttt cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa
    17761 tctttccctc tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa
    17821 ttatcagcaa gacgttcgat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc
    17881 ttacgtgacc aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc
    17941 gccaccgatt cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc
    18001 agaatttccg gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg
    18061 tagtggaagt agacttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgtccg
    18121 atgctgttac cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc
    18181 gttgccagtt cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg
    18241 ctggcggtat gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga
    18301 tgtactgcaa tggtcggccc ttctgccaga atattcagat tggcatcggt caccagcgtg
    18361 gcaatctcaa taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa
    18421 atcaggttgt tttcattggc actcatgcta ttttccaccc attcacagtg actatattga
    18481 tccacaattg cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtcagtacga
    18541 ttgagtaaaa ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt
    18601 cgtcttaaaa cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat
    18661 gaaggtatcg tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac
    18721 gttcaccgct gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc
    18781 tggcgtccgg gtaaaccggc ggcggaaggc gtaaatatca aagggatcgt ggaagcggtg
    18841 catgagcgca cctcggtgtt aacgcgtccg gatttctacg acggcgtgaa acccatagcc
    18901 gccaacatcg accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt
    18961 atcgaccgtt acctggtggc ctgcgaaacc ttgcagattg agccgattat cgtgctcaac
    19021 aagatagacc tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac
    19081 cgcaacatcg gttatcgcgt attgatggtt tccagccata ctcaggatgg gctgaaaccg
    19141 ctggaagagg cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggtaaa
    19201 tccagcctgc tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct
    19261 gacaactcgg ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac
    19321 ggcggtgatg tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg
    19381 gaacaaatca ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc
    19441 gattgcaaac acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa
    19501 atcgcggaaa cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa
    19561 acgcgtaaaa acttttctga tacggatggc tgacaattaa gctaaccgtc gttagaatcg
    19621 tccccttttt ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg
    19681 gaggctacct tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg
    19741 cttactcgcc tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt
    19801 atcgatctgt tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc
    19861 gccagctacc gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca
    19921 atcgataccg atccgaatgt actggtcatg cctgccgatg gcgttatcag ccagttgggt
    19981 aaaatcgaag aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg
    20041 ctggcaggca actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac
    20101 ctctccccgc gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg
    20161 atctacgtgc cgggcgatct cttttccgtt aaccatctca ccgctcagaa cgtgccgaat
    20221 ctgtttgccc gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg
    20281 cagattctgg tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt
    20341 acgccgccgc gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt
    20401 tctgtggcac tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc
    20461 aacctgtttg caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg
    20521 aaaattggcc agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc
    20581 gccccacttc ctgctgaaga gatcgaagca gaacacgacg ccaggccatt ggttgacgat
    20641 aaaaaagacc aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt
    20701 ctgatggcct ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa
    20761 atcactcagg aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag
    20821 gcgctccagt ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa
    20881 caatatcagc aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta
    20941 aacaacatgc gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat
    21001 caggaaattc tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag
    21061 caggagcgcg cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac
    21121 gcccgccgtc agttaaatga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg
    21181 ctcaatcagg cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt
    21241 gatgaactgg agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttacgc
    21301 tcagagctgg cggaaaaaga gagccagcaa ctggatgcgt atttgcaggg cttacgtaat
    21361 cagttgaata gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg
    21421 gcagaaaaca gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa
    21481 ctatcggcag cgctgaatca acaggcgcag cggatggagc tcgttgcctc gcaacagcgt
    21541 caggctgcca gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg
    21601 caatggctgg gatcgtccaa cctgctcggc gaagcgctgc gggcgcaggt ggcacggcta
    21661 ccagaaatgc caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg
    21721 ttacgttatg aggatttact taataaacag ccgctgctac ggcaaatcca tcaggccgac
    21781 ggtcagccgc tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt
    21841 gagttgctga actcattgtt gcagggtggc gacacgctac tattggaact gaccaagctg
    21901 aaagtctcca acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc
    21961 tatctgttct ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc
    22021 caggatctgc gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg
    22081 atgatgctga ccagcaaaga gacaattttg ccgctgtttg gcgcgttgat tctggtcggt
    22141 tgcagtattt actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc
    22201 ggcaaagtga ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc
    22261 gtcgcgtcac cgttaccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg
    22321 ccttatccgc tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg
    22381 gtagtgatga tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc
    22441 tggccgcgcg aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggctgatt
    22501 atggcgctga tgatgttcga taacctcgac gaccgcgaat tttccggttc gctgggacgg
    22561 ctttgcttta tcctcatttg cggtgcgctg gcggtggtca ccctaagcct gaaaaaagcc
    22621 ggtattccgt tgtatctcaa caaagaaggc agcggcgaca acattaccaa ccatatgctg
    22681 tggaacatga tgattggcgc accactggtt gccattctgg cgtcggcggt gggttatctg
    22741 gcaacggcgc aggcactgtt agcgaggctt gaaacctcga ttgccatctg gttcctgcta
    22801 ctggtggttt atcacgttat ccgccgctgg atgctgatcc agcgtcgcag gctggcgttt
    22861 gaccgggcga agcatcgccg ggcagagatg ttagcgcaac gtgcgcgtgg cgaagaggaa
    22921 gcgcatcatc acagtagccc ggaaggagca attgaagtcg atgaaagcga agtcgatctc
    22981 gatgccatca gtgcgcaatc cttgcggctg gtgcgctcaa ttttgatgtt gatcgccctg
    23041 ctttcggtca ttgtgctgtg gtcagaaatc cattccgctt tcggcttcct cgaaaatatt
    23101 tcgctgtggg atgtcacctc cacggtacag ggcgtagaaa gtctggagcc aattaccctc
    23161 ggtgcggtgc tgattgccat tctggtattt atcatcacca cgcagctggt gcgcaacctg
    23221 cccgcgctgc tggaactggc gattttgcag cacctggatt taacgccggg tacgggttac
    23281 gccatcacca ccatcacaaa atatctgctg atgctgattg gcgggctggt cggcttctca
    23341 atgattggta ttgagtggtc gaaattgcag tggctggttg ccgcgctcgg tgttggtctc
    23401 ggttttggtt tgcaggaaat tttcgccaac tttatctctg gcctgatcat cctgttcgaa
    23461 aaaccgattc gcattggcga tacggtaacg atccgcgatc ttactggtag cgtgacaaaa
    23521 attaacaccc gcgccaccac catcagcgac tgggaccgta aagagataat cgtgccgaac
    23581 aaggcgttta ttaccgagca gtttatcaac tggtcgctct ctgactcggt cacgcgcgta
    23641 gtgttgacga ttccggcccc tgccgatgcc aacagtgaag aagtgacgga aatcctgctc
    23701 accgcagcgc gtcgctgctc gctggtgatc gacaacccgg caccggaagt cttcctggtg
    23761 gatctgcaac aggggattca gattttcgag ctgcgtattt acgccgctga gatgggtcac
    23821 cgtatgccgc tacgccatga gatccaccag ctgattctgg caggcttcca cgcccacggt
    23881 atcgatatgc cattcccgcc cttccagatg cgtctggaaa gcctcaacgg taaacaaacg
    23941 gggagaacgc tgacgtctgc gggcaaaggt cgtcaggcgg gaagtttgta atgcatgaag
    24001 ctgccaaatg atgtcaacgt ggctcattta gcagcttaac atcatcttca gttatcaccg
    24061 gccctggaga gtatcatcca tacgcattac ttcctgacaa ctcatcgccg gagagacgat
    24121 gagttgcagg atttcatatg atcagtcgag agctaactca ggtttattcg ccatgcgact
    24181 gcaatagttc tggtaaatcg ccatcgctaa cagcgagaag aagacagggc cgccaatcat
    24241 ccacaatgtg ctgtcccagt ctccggcttc aaccacaggc tgaataatgg tgaagacgtt
    24301 cgcaaatgtc accaccagaa cgacaaccac cgtcgcaatc attgccgaca tacgcgtttt
    24361 gaaaatcaca aatggtctgt cgagatcctg acgtgcttta aaaaacggga aagccagcgc
    24421 gaggaacagg taaggaagcg tcatagacac gttcgccatc agcgtcagct tgttaaagaa
    24481 cgccgatgcg gtaccgccac caaacgaaac cagcagaatg aagacagtaa ccaacccgca
    24541 ctgcatccac atggcgatag acggcatccc catcgcattc aggcgcgtca tcggttccgg
    24601 ccacaacgct ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa
    24661 gaacgcaccg gtataagcga ggaacatcga aagtccggta atacgcgcaa accatacgcc
    24721 cagcgacaat gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct
    24781 cttcatcagc acataggtaa tattgccaag gttaacggaa ccattactta atacctgctg
    24841 ccagtttgtg ctgacgcccc ataaaaatat tgccagcgaa taaccgattg aaataacaat
    24901 agcggcaaaa acaatacctt tggcaaagtt cttttctgga ttttccgttt tatcgaccag
    24961 accaccaacc gcttcaattc cgccataggc aaaaatagca aataccacaa acgatagcat
    25021 tgccagaccg gactgataac ccgggttcgg tgatgcaagg aaattaatat cctgcgcgaa
    25081 atgcccacca tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca
    25141 cattactgca ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc
    25201 aacgacggtg accagaatca tccatgctac tgccagcaga ccaaccacct gcgtaggctc
    25261 cagtccggca atacgccagt gctgggtcat gtcactacca tagaggaatg ttgagaacgg
    25321 tacccaaact ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa
    25381 cgtaccaatg aaggcaaaac gtggtccgac actattattc atccaggaat agataccacc
    25441 ttcttctttg cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa
    25501 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
    25561 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
    25621 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
    25681 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
    25741 taccttgatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
    25801 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
    25861 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
    25921 aacatcacca gacgatcaac acctaatgcc acgccggagc agtcaggcat accgactttc
    25981 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
    26041 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
    26101 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
    26161 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
    26221 tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
    26281 tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc
    26341 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
    26401 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
    26461 aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga
    26521 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca
    26581 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
    26641 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
    26701 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
    26761 cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc
    26821 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
    26881 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
    26941 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
    27001 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
    27061 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
    27121 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
    27181 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
    27241 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
    27301 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
    27361 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
    27421 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc
    27481 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
    27541 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
    27601 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
    27661 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgttggtg cagatccgtg
    27721 ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg
    27781 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
    27841 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
    27901 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
    27961 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
    28021 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
    28081 ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc
    28141 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
    28201 aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc
    28261 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
    28321 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
    28381 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
    28441 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
    28501 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
    28561 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
    28621 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
    28681 ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
    28741 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
    28801 tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt
    28861 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
    28921 atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa
    28981 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
    29041 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
    29101 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
    29161 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
    29221 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
    29281 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
    29341 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
    29401 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
    29461 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
    29521 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt
    29581 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
    29641 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
    29701 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
    29761 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
    29821 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
    29881 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
    29941 gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
    30001 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
    30061 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
    30121 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
    30181 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
    30241 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
    30301 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
    30361 gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct
    30421 acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca
    30481 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
    30541 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
    30601 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
    30661 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cttggggcta
    30721 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
    30781 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
    30841 aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac
    30901 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
    30961 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggccccagg
    31021 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
    31081 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
    31141 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
    31201 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
    31261 gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga
    31321 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
    31381 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
    31441 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
    31501 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
    31561 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
    31621 caatccagag agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
    31681 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
    31741 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
    31801 ttctggtcgt tgcctgtagt tctcctacgc cgccgcgtgg cgtgaccgta gtgaataatt
    31861 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
    31921 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
    31981 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
    32041 actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
    32101 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg
    32161 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
    32221 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
    32281 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
    32341 gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
    32401 cgccgactgc gccaataccc gtccacacgg catcaaacgg ctaaagccgt gggtatattt
    32461 caggccaacg gcccatacca cttccagcag accagcaata actaagataa tccaggacat
    32521 atcaggctcc ggaaatttgg ggccgtcccc ggtgaaaaga ggcgtttgca ggtcgtcctg
    32581 caaagctgat ggttgaacgg atattttgcc aggtggcagg ggaatttcaa ctattacctt
    32641 tatgacgatc gcagaaaaaa caaacggcac gacactgctg tcgtaccgtt attgcttatt
    32701 gctgcgcttt cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa
    32761 cgccacgcgt ggtgttgcag gcagttaata ctgttgaaag caccagaaca gaaaagatcg
    32821 ctgcaattgt cttcttcacc ataacgtctt ccttttaggt aatgttattt atgtttgcct
    32881 atagcaaatt aataatagac aacatcgcca aaaatgatgg aaatctgatg atttttcgga
    32941 agagaagaaa aattagctgg cagcgcgaga gatggagttg ccgagatgtt tgatgtcttc
    33001 gccgaaaccg caagcggtgt tacagcccgt gagcagcgtg ctggcaagca aaacaagaac
    33061 gataaggcgt ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc
    33121 acaaccgcat tacttcacgc gagagacgta ttcaccagag cgggtatcca ctttgatgac
    33181 ttcgccgatt tgtacgaaca gcggaacttt aaccacagcg ccagtagaca gggtagccgg
    33241 tttgccgcca gtacctgcgg tatcaccttt caggcccgga tcggtatcaa cgatttccag
    33301 ttcaacgaag ttcggcggag taacggagat cggctgacca ttccacagag ttacgataca
    33361 ctctgcctga tccagcagcc atttagcgtt gtcgccaatt gcttttgcat cagcagacag
    33421 ctgctcgaaa gtttcgttgt tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt
    33481 caggttcata tcgacaacat cagcgccttc agcggaatca gtagatttga aggttttttc
    33541 tacgcgagta ccggtcagca gacgacgcag tttaacgcga gcaaatgcct ggcctttacc
    33601 cggttttacg aattcactcg cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag
    33661 accagcacga aaatcgttgc tatagtacgt tgccataagg ccctctgaaa tttgttaatt
    33721 ggtagctaag ccacaaaatg gcgcatattg taaccctaaa taccccatcc agagaagatt
    33781 ggttaacgca acttgccgat gttgtgaccg atcctgatga acttctgcgt cttttgaata
    33841 tagacgcgga cgaaaaactg ttggccggac gcagcgccaa aaagctgttt gccctgcgtg
    33901 tgccccgctc atttatcgat cgcatggaga aaggcaatcc ggacgatcct cttttgcgtc
    33961 aggtacttac ctcgcaagat gagtttgtcg tcgcgtccgg attctccacc gacccactgg
    34021 aagaacagca cagcgtagtg cctggtttgt tgcataaata ccacaaccgg gcgcttttgc
    34081 tggtcaaagg cggctgcgcg gtaaattgcc gctattgctt ccgtcgccac ttcccctatg
    34141 ccgaaaatca gggcaacaag cgtaactggc agactgcact tgagtatgtt gctgcgcatc
    34201 cggaactgga cgagatgatt ttctccggcg gcgatccgct gatggcgaaa gatcacgagc
    34261 tggactggtt gctcacacaa ctggaagcca tcccgcatat caaacgtctg cggattcaca
    34321 gccgtctgcc gattgtgatc ccggcacgta tcaccgaggc gctggttgaa cgctttgccc
    34381 gttctacgct gcaaatcttg ctggtgaatc acatcaatca tgccaatgag gtagatgaaa
    34441 cgttccgtca ggcgatggct aaattgcgcc gtgtcggtgt caccctgctt aaccagagcg
    34501 ttctgttacg tggtgtgaac gataacgcac aaacgttggc aaacctgagt aatgcgttgt
    34561 tcgatgccgg cgtgatgccg tattacctgc atgtgctaga taaagtacag ggcgcggcgc
    34621 attttatggt gagtgatgac gaagcacggc agattatgcg tgagttgctg acattggtgt
    34681 cgggatatct ggtgccgaaa ctggcgcgag aaattggcgg cgaacccagc aaaacgccgc
    34741 tggatctcca gctacgccag cagtaactga ttaaacaggg aagttaaaca ttaaaattgc
    34801 ctgatgcgct acgcttatca ggcctacatc gtgcattgaa attgaatgga tttgcaatga
    34861 actgtaggcc ggataaggcg tttacgccgc atccggcaag agcaacaaac gcaagtacag
    34921 caacctgtca ttaaactgag aaatttaatc tttgcacgtt gcataacctt taattattca
    34981 tatgcattgc tgttgttact ttttatatag ctaacaccaa taattaacct ttctttt
//
LOCUS       DAJTNM010000026        34538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTNM010000026 DAJTNM010000000
VERSION     DAJTNM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.026"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..245
                     /locus_tag="OO172_001409"
     CDS             <1..245
                     /locus_tag="OO172_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5511646.1"
                     /translation="LAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNG
                     ARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
     gene            complement(289..4906)
                     /locus_tag="OO172_001410"
                     /pseudo
     CDS             complement(289..4906)
                     /locus_tag="OO172_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(4999..5646)
                     /gene="rnt"
                     /locus_tag="OO172_001411"
     CDS             complement(4999..5646)
                     /gene="rnt"
                     /locus_tag="OO172_001411"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5511647.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5749..6156)
                     /gene="gloA"
                     /locus_tag="OO172_001412"
     CDS             complement(5749..6156)
                     /gene="gloA"
                     /locus_tag="OO172_001412"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5511648.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6237..7334)
                     /gene="nemA"
                     /locus_tag="OO172_001413"
     CDS             complement(6237..7334)
                     /gene="nemA"
                     /locus_tag="OO172_001413"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5511649.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7371..7970)
                     /gene="nemR"
                     /locus_tag="OO172_001414"
     CDS             complement(7371..7970)
                     /gene="nemR"
                     /locus_tag="OO172_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5511650.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8073..8312
                     /locus_tag="OO172_001415"
     CDS             8073..8312
                     /locus_tag="OO172_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5511651.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8361..9257
                     /locus_tag="OO172_001416"
     CDS             8361..9257
                     /locus_tag="OO172_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5511652.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9338..9667
                     /locus_tag="OO172_001417"
                     /pseudo
     CDS             9338..9667
                     /locus_tag="OO172_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
     gene            complement(9638..10021)
                     /locus_tag="OO172_001418"
                     /pseudo
     CDS             complement(9638..10021)
                     /locus_tag="OO172_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(10014..10805)
                     /locus_tag="OO172_001419"
                     /pseudo
     CDS             complement(10014..10805)
                     /locus_tag="OO172_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(10808..11044)
                     /locus_tag="OO172_001420"
     CDS             complement(10808..11044)
                     /locus_tag="OO172_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5511653.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            11245..11679
                     /gene="slyA"
                     /locus_tag="OO172_001421"
     CDS             11245..11679
                     /gene="slyA"
                     /locus_tag="OO172_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5511654.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(11726..12193)
                     /gene="slyB"
                     /locus_tag="OO172_001422"
     CDS             complement(11726..12193)
                     /gene="slyB"
                     /locus_tag="OO172_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5511655.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            12467..13576
                     /gene="anmK"
                     /locus_tag="OO172_001423"
     CDS             12467..13576
                     /gene="anmK"
                     /locus_tag="OO172_001423"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5511656.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            13674..14003
                     /gene="mliC"
                     /locus_tag="OO172_001424"
     CDS             13674..14003
                     /gene="mliC"
                     /locus_tag="OO172_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5511657.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            14062..14718
                     /gene="pdxH"
                     /locus_tag="OO172_001425"
     CDS             14062..14718
                     /gene="pdxH"
                     /locus_tag="OO172_001425"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5511658.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            14847..16121
                     /gene="tyrS"
                     /locus_tag="OO172_001426"
     CDS             14847..16121
                     /gene="tyrS"
                     /locus_tag="OO172_001426"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5511659.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            16180..17049
                     /gene="pdxY"
                     /locus_tag="OO172_001427"
     CDS             16180..17049
                     /gene="pdxY"
                     /locus_tag="OO172_001427"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5511660.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQV
                     KAANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAV
                     NNVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGM
                     RQPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDR
                     IAKPEHYFSATKL"
     gene            complement(17093..17698)
                     /gene="gstA"
                     /locus_tag="OO172_001428"
     CDS             complement(17093..17698)
                     /gene="gstA"
                     /locus_tag="OO172_001428"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5511661.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(17804..19306)
                     /gene="dtpA"
                     /locus_tag="OO172_001429"
     CDS             complement(17804..19306)
                     /gene="dtpA"
                     /locus_tag="OO172_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5511662.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(19917..20552)
                     /gene="nth"
                     /locus_tag="OO172_001430"
     CDS             complement(19917..20552)
                     /gene="nth"
                     /locus_tag="OO172_001430"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5511663.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(20552..21247)
                     /gene="rsxE"
                     /locus_tag="OO172_001431"
     CDS             complement(20552..21247)
                     /gene="rsxE"
                     /locus_tag="OO172_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5511664.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(21251..21871)
                     /gene="rsxG"
                     /locus_tag="OO172_001432"
     CDS             complement(21251..21871)
                     /gene="rsxG"
                     /locus_tag="OO172_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5511665.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(21875..22933)
                     /gene="rsxD"
                     /locus_tag="OO172_001433"
     CDS             complement(21875..22933)
                     /gene="rsxD"
                     /locus_tag="OO172_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5511666.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(22934..25060)
                     /gene="rsxC"
                     /locus_tag="OO172_001434"
     CDS             complement(22934..25060)
                     /gene="rsxC"
                     /locus_tag="OO172_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5511667.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(25053..25631)
                     /gene="rsxB"
                     /locus_tag="OO172_001435"
     CDS             complement(25053..25631)
                     /gene="rsxB"
                     /locus_tag="OO172_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5511668.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(25631..26212)
                     /gene="rsxA"
                     /locus_tag="OO172_001436"
     CDS             complement(25631..26212)
                     /gene="rsxA"
                     /locus_tag="OO172_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5511669.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFATIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(26289..26729)
                     /locus_tag="OO172_001437"
     CDS             complement(26289..26729)
                     /locus_tag="OO172_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5511670.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(26815..27030)
                     /gene="ydgT"
                     /locus_tag="OO172_001438"
     CDS             complement(26815..27030)
                     /gene="ydgT"
                     /locus_tag="OO172_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5511671.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(27303..27428)
                     /gene="blr"
                     /locus_tag="OO172_001439"
     CDS             complement(27303..27428)
                     /gene="blr"
                     /locus_tag="OO172_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5511672.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            27671..28711
                     /locus_tag="OO172_001440"
     CDS             27671..28711
                     /locus_tag="OO172_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5511673.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(28747..29748)
                     /gene="add"
                     /locus_tag="OO172_001441"
     CDS             complement(28747..29748)
                     /gene="add"
                     /locus_tag="OO172_001441"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5511674.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(29851..31023)
                     /gene="malY"
                     /locus_tag="OO172_001442"
     CDS             complement(29851..31023)
                     /gene="malY"
                     /locus_tag="OO172_001442"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5511675.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(31033..32625)
                     /gene="malX"
                     /locus_tag="OO172_001443"
     CDS             complement(31033..32625)
                     /gene="malX"
                     /locus_tag="OO172_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5511676.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            32800..33828
                     /gene="malI"
                     /locus_tag="OO172_001444"
     CDS             32800..33828
                     /gene="malI"
                     /locus_tag="OO172_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5511677.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            33940..>34538
                     /gene="hdhA"
                     /locus_tag="OO172_001445"
     CDS             33940..>34538
                     /gene="hdhA"
                     /locus_tag="OO172_001445"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5511678.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFSWRCDITSEQELSALADFAVSKLGKVDILVNNVGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSV"
BASE COUNT         8599 a         8861 c         8976 g         8102 t
ORIGIN      
        1 tgcttgctgt tccgccagcg tacacaagcc agcgttgcgc gtgctgtggt catacagcga
       61 aagaaaaccg cctgtcacaa agtaaattca gatgccaggt atgtggatat acagcgaacg
      121 ccgatgtaaa tggcgctcgt aacattttag cggcggggca cgccgttctt gcctgtggag
      181 agatggtgca gtcaggccgc tcgttgaagc aggaacccac cgaaatgatt caggcgacag
      241 cttgaacgta gcagggaacc acgtctttca gggcgtggag gatgtcaact atccccaatc
      301 cagcccttgt ggcgaacttg aaaatcccgc ctcacgcagc agcgtaaaca tcggcgtttg
      361 ccggaccgga agatcattca cttctgttag cgtaaagcgc agccttggtt cgcgacgcag
      421 tgcggtggtc agcgcctgga aaatctctgg ggcgagtaat tcctcctttt cctgccagac
      481 cagcattttt ttgccgcctt gcggcagata gagtaacaat ttgccgccag aaacgaccac
      541 cagcgcaccc gcccgacgcg ttggtaccag cgctgccgga tgtgcaggcc agggcagaag
      601 atttccccac acattagccg gatcgttggc ggagagcgcc actggtgtat agtggcgtgt
      661 ttgcgtggct tgtgtcgcca gatcgcgcaa tcggtcaata gtcagacgtt cagcgaattg
      721 cgcgccaccc agaccttcta caaaacgacc tcgcataatt cgcccggagt cttccatact
      781 tcgacaaagt gtttgcatcg atggaaaccc gccagggata ttttcggcta tcaccgcctg
      841 acgactgatg atgccgtagc ggtcgagcat attttccgcc agcgccagca tcctttcggt
      901 atcgtttagt ggctccacct gcaataacga ccagcgtcca gccagatttg gtgtgttgta
      961 agatacccgc ggcgagacgg gttgtgcata gacaggacgc ccacgacgag cccggtgact
     1021 gcggcgagtt gaggtgcgtg cgttggaact gctgcgggtg agggcgcgta acggtgccca
     1081 aatatcgctg gtaatgacgc cttgccagac cagcgcccat aacgcctctt gcagtgccga
     1141 ggaatcaacc gattcgccga ttttatcgcg tatccgttgg ctgatttgtt gtgcaaacca
     1201 ggctcctccg tcagccagaa cagcgattat cgcttgctgc agcgccgaac gattcgcctg
     1261 atccgcttcg gcgggagtga acgattctgc ggcatatttc tgtagatgca gtgccaccag
     1321 gccgtcatct tcacccagct tttttttgtc ccgaccagat aaccgctccg gttgccagta
     1381 attcatcgag catttctggc gaatagtcgc gtacgcgggc aggcaggatc tggctttccc
     1441 agagtgaggc gggtaaaccg actccggcaa gctgttcgat cacccgcatc acgccatcta
     1501 cgccctcata aacgcctggc gatgttgagg caaagagcgc cgggctacca tcggtggcgg
     1561 gtaatacgcc ctgacgttcc agcagcaatc gcgcataggt cgtggctgca acgggacgcg
     1621 tcgcttctct ggcggcttgc agcgagcgca aacgcagacg acgaaatact tcatcgctca
     1681 cccagatgtc ttgttgcaga ttcatcacca gaccctgttc acgcagttgc tgaagctgct
     1741 cttcgacaat ggcaataccg agactaaact catgagccag ttgttcagcc gtgaccagag
     1801 catgagcccg gagatagcgc agaaagaggt cgcgaagcgg gtaactgact ctatgtaaat
     1861 aaatctctgg caatgactct ggtagtcgta cgccgagggc atcacgcagc ctggcggcat
     1921 catccataca ggcaagacgc tcctgtccgc taatcatcgc agggaagatt cgttttactg
     1981 caagaagatt ttccagatac gacgcaacct cttcactgct gcctgtatgc cgttgcgcca
     2041 ggtcttcaac ggtcattggc cccagttcgc gcagcaggtc gaacaatcct tcttcacctt
     2101 tcgctcttct gccaggagcc agtcgttgca actcttcttc cacctggcga atgacctgcg
     2161 ggtcgagtaa ttcccccgga tcgacctgtc cgagtagatt gcgcagtaac tcgctgtcca
     2221 gcgacagtac ggatgcccgg cgctctgcca gcggggcgtc gctctggtac ataaattccg
     2281 cgacatagcc gaacaataaa cttgtggcga aaggcgaggg cgtagtggtc gttatatcgg
     2341 atatttgaat ttcgccaccg ttcaggcgac gcatcaaacg ttccagtgcg ggaagatcat
     2401 aaacatcttg cagacattcg cgtagggttt cgagaatgac cggaaaatcc ggatatccct
     2461 gagcgatttc cagcaactga ctggcgcgca gccgttgttg ccaaagcggg gtgcgatggc
     2521 ccggcgtgcg gcccggcatt aataatgccc gcgcggcgca ttcgcgaaaa cgggcggcga
     2581 aaagtgccga gctgcctacc gcctcgcgga caatttgcag caacttttct ggttcaaaca
     2641 aaaaaatcgc ggcgtcgggc aatttaccat cagtgtcagg aatgcgcgca acaatgccgt
     2701 catcactggc gaccacagac gcgtcagcgc cccatagggc atgtattcgc ccggcaatcg
     2761 ccagcgccca gggttcatgc acccgtcttc catagggaga gtgcaaaata atacgccagt
     2821 cgccaatttc atcacggcac cgttcgagga ccagatggcg actccccgga acgatgcccg
     2881 tcgcgttgcg ttgctcgtca atcaacccct gaatattggc gttcgtattt tcttctgcca
     2941 gccacggggg aatagtcccg gaaaagaacg cgccatccgc cagtaaatga agaaaatcgc
     3001 cgatcatctc gcctaattca gccggacgtc cgttaccttc accacgccag aaggggagcc
     3061 gggcagaacg acccggagca ggagtcacaa tcacctgatc gcgggtgatt tgctggatcc
     3121 gccatgaggt agcgccgaga gtgataatgt cgttcacccg cgactcatat accatctcct
     3181 catccagttc acccacccgc cgcgaaccgg ccttttcttc accttcgggt aataacacgc
     3241 tatacatgcc acgatccgga atggtgccgc cgctggtaac cgccaacaat tgagcgccag
     3301 gtcgggcggt caatatcccg gtctccctgt tccagaccag tttggggcga aaagcagaaa
     3361 aatcgccaga gggatagcgc ccggaaagca tatccagcgt ggcgtcaaaa acacgtcttg
     3421 gcagatcttt ccacggtgcg gcacggcgta cgcgggagta ccattcgtct acctgtaatg
     3481 catccatcgc cgcggcggca acggtttgct gcgcaaggac gtcgagagga ttatgcggtg
     3541 gtgtcaggtt ttccagcctg cctgcgaaca tacactctac aatgactgcg gaatcgacta
     3601 aatcacgccg ggtacggggg aaaaacagcc ctttagatac accgccaacc tgatgtccgg
     3661 cgcgaccaat gcgttgtaac ccactggcaa cagaaagcgg cgttgccacc tgaatcacca
     3721 gatccaccgc gcccatatca atccccagtt caagactgga ggttgcgacc acgcagcgta
     3781 attcacccga tttcagcgcc tgttcggtga ttgctcgttg ttctttagag acggagccgt
     3841 ggtgtgagcg ggcaataaaa acgtcgctac tttgtacacg gttagaggtt gcgccggagg
     3901 tcgactcgaa atgggccgca tcaacggcga tagacgggga acgctgtaag cgtgcggcgt
     3961 aaagctcatt taatcgtgcc gtcagttttt ccgccagccc gcgtgaatta gtaaagacaa
     4021 tggtcgagcg atggcgcaac acttcatcaa ggatacccgt ttcaatatat ggccagatgg
     4081 agccttcccg gccggcatgg ctgtcttcgc cggtgccgct ggcgaccgat gagacatcat
     4141 ccatattggc gaccggtacg acaattcgta tctgcggatg gcgcattgcg ggcgggttga
     4201 ctaccgtaac cgggcgatcg ccaccaagaa atgctgccac atcgctggct gagcgcacag
     4261 tggcagaaag gccaattcgc tgtgctgagg tgtggagcag cgcatcgagc cgctccagac
     4321 ttaacgccag atgcgcacca cgtttactgc ccgctaccgc gtggacttca tcaataatta
     4381 ccgtttcgac gccgtgtagc gtttcgcgcg cgcgggaggt cagcatcaga tagagtgatt
     4441 cgggtgtggt gatcagaata tccggcggat tacgggtgag tttgctgcgt tcctgtgcag
     4501 gcgtatcgcc agtacggatc cctacgcgaa gattgacttc cgtttcgccg cgccgccgcc
     4561 gttcatcggc aatacccttc aacaggatct gcaagttgcg ctgaacgtcg gtgcccaggg
     4621 cttttatcgg tgaaatatag aggatgcgtg aggttttacg cttatgcgcc tcgcgggtat
     4681 cttcgccgcc ttcgcggaag agccgatcga gggcgtagag aaatgctgcc agcgttttcc
     4741 cggagccagt cggtgcaatc accagcgcat gttcgcctcg cgccgccaca tgccaggttt
     4801 gcgactggac agcggtcggc tgtttaaagg cgcgaagaaa ccagtcgcgg gtcgccggtg
     4861 aaaacacgtc cggcaggagc gatgaagggt ctggattatc tgccattaac gctattcccc
     4921 gtgaatatca tgcttgatgc gtggaatagc ctcactatag atgcctgaat cattgcatgt
     4981 catcaggcat cgactcgatt acacctcttc ggcggcaggt agcggccagc ctcccagacg
     5041 tttccagcgg ttgacgattt cacaaaacag cacagcagtg cgttcggtgt cgtacagcgc
     5101 ggagtgcgcc tgggtgctgt cgaagtccat gccagcggtc tggcaagcct ttgacaatac
     5161 ggtttgtccg agtgccagcc cggccagtgc agcagtgtca aaagtggcga aagggtggaa
     5221 cgggttacgt ttcagcgagg cgcgttctgc ggcggccatc ataaagctgt gatcaaaatt
     5281 ggcgttgtgc gccaccataa tggcgcggtt acagccgctc gctttaatac ctttacgtac
     5341 aactttaaaa atttcgtgca gcgcctcgta ttcgctgacc gcgccgcgat cgggatcgtt
     5401 cgggtcaatg ccgttgaagg cgagggcttc tggttgcaaa tttgcgccga caaatggttc
     5461 gacgtggaaa tgtaatgtgg tgtccggcat cagccagcct tgttcatcca ttttcagggt
     5521 gatggcggca atctcaagca gcgcatcggt tttggcgtta aatccggctg tttcaacatc
     5581 gatcacaaca ggataaaaac cacgaaaacg gtcgcacaga ccggtaagtt gagcgttatc
     5641 ggacatctga ttctcttaat acggataaat tgcagcgcgc attatgacaa atattacttg
     5701 tcgatgcagt aaagatgcgg gcgcgatgag ttcacgcccg gcaggagatt agttgcccag
     5761 accgcgaccg gcgtctttct cttcgattaa ctcaattttg taaccgtccg gatcttccac
     5821 aaacgcgata accgtagtac cgccttttac cggacccgcc tcacgggtca cgttaccccc
     5881 gttttgacgg attttttcgc acgcttcagc ggcgttatct acgctaagcg cgatgtgacc
     5941 ataagcagtg ccgagttcgt atttatccac gccccagttg taggtcagtt caatcaccgc
     6001 ttcttcggtt tccgggccgt agccaacaaa cgccagtgag tatttgtatt ccgggttttc
     6061 gctggtacgc agcagtttca tgcccagcac tttggtataa aaatcgatgg agcgttgcaa
     6121 atcgccaacg cgcagcatgg tatgaagaag acgcatcttt ttatcctcaa aatggctaac
     6181 agattcaaaa tgttgtttta gtatagcggc ggctttacgc cgctcgcaat gttggattac
     6241 aacgtcgggt aatcggtata gccttccgcg ccgccaccgt agaaactttc ggcacgctgt
     6301 gggttaagct cagctttgag ctgcaagcgg gcgaccagat ccgggttcgc aatccagtca
     6361 cgaccaaatg ccaccgcatc aattaaccct ttgccgatca gcgtttcagc tttttctact
     6421 gtgtatgcac ctgcgccgat aatcggaccg tggaaacggg cgcgtacttt ttcgcggaac
     6481 gcatcagtat acggttcacc ccccgcccaa tctggttctg acatatgcag ataagcaatg
     6541 ccgcgtttac ccagttgttc aatcagatac agtgcatcgg cttcttcatt cgggccgtta
     6601 tctgtgttct ggaaagtacc gattggcgaa acgcgaatgc caatgcgatc ggcaccccat
     6661 tcttcaatcc cggcatcgac cacttccagt accaaacgtg cgcgattttc cacgctgccg
     6721 ccgtactgat cggtacgatg gtttgaagaa ggggaaagga actgatgcag caaataaccg
     6781 tgagcagagt ggagctctac cagatcaaaa ccggcttcac gcgcgttagc aatggcctga
     6841 cggaaatcat tgacgatacc cggaatctct tccagttcaa gcgcacgcgg catggatgtt
     6901 tcaacgcgga tcgcccgacc attttcatcg cgcagagaag tacgtgttcc cgcgctaagt
     6961 gctgaaggcg ctaccggtgc ctgaccgcca ggttgcaggc tggcgtgaga aatgcgtccg
     7021 gtgtgccaca gctgcaaggc catatgacca ttttcagcat gaacgccagc ggtgattttt
     7081 ttccatgcgg caatttgctc cggactatgg atgccaggcg cacctgcata tccttttgcc
     7141 tgggcagaaa tttgcgtggc ttcactaata atcaaaccgg cactggcacg ttggcgatag
     7201 tattccgcca tcaacggggt aggaatgtca cccggttcaa tactgcgcag acgcgtcagc
     7261 ggtgccataa aaatacggtt tgccgccgtg atcgcgccca ctttcagtgg ggaatacagt
     7321 ttttcagatg acataaaggc tcccggtcgt ctggtgataa aaaatgcctg ctaaacggca
     7381 ggcgtcgcaa taatgttttt tacatgggcc agcgcgtttt ccagtggctc gaaactgcgc
     7441 gaaattttgg cctgcagatt cgcgccaagc catagtgcgt aaagcacttg tgcctgttgt
     7501 agcggttcgc cacaaaaggt taaacaatgg ttctcacggc cattttccag agcctgcgag
     7561 agcagggcga tcacgccgcg agcaccttta tccatcgcgc tgcgcatatc ttctgacaga
     7621 tcgcacactt cggcagagag ttttactgtc aggcaaccac tgatggttcc atgttggcaa
     7681 aactggttca gtgtttgctg gtaataagcc agtatgcggt cgcggtagtt accttcgccg
     7741 gattgcagca actcagccag tcgctggtga tatgcggcgt aatgacgctc aagcatggca
     7801 acgccaaacg cttctttaga gcgaaagtag tgatagaagg accctttcgg cacttcagcg
     7861 gtttttagta attcgcttag ccccatcccg gtgaatccac gttgcaggca aagttgctcg
     7921 cccgtcgcca agagatgttc gcgagtatca tgttcggtgt gtttgttcat gcgttggagt
     7981 gtagtagacc agtcggtcta gtgcaagcaa agttgctact ttggacatta acagcttcaa
     8041 taatggtaat aatcttatct gaggagggcg aggtggcgga gcaattagag ttctttcccg
     8101 tccagagccc gtgccgggga atttgccagt ctgacgaacg cgggttttgt cgcggctgtt
     8161 ttcgtagtcg ggatgagcgt tttaactgga ataaaatgag cgatggcgaa aaacaagagg
     8221 tgctgcgcct ttgtcggcag cgattaatgc gcaaattacg cgcaaataag ccagcgtcat
     8281 cagacgaacc ggagcaacca tcactctttt gaacctaatc ttgcgtatac tcctctcata
     8341 tctgataaga ggaagcgatt atggttcagc gtattactat tgcgccggaa ggcccggagt
     8401 tttcccgttt tgtgatgggc tactggcgat tgatggactg gaatatgtcc gcccgccagc
     8461 tggtcagttt tattgaagag catctggatc tcggcgtgac caccgtggac catgctgata
     8521 tttatggtgg ctatcagtgc gaagcggcgt ttggcgaggc actgaaactg gcacctcacc
     8581 tgcgtgaacg gatggaaatc gtcagtaaat gcggtatcgc gacgaccgcg cgtgaagaaa
     8641 acgtcattgg tcattacatc actgaccgca atcacatcat taagagcgcc gaacagtcgc
     8701 taattaatct cgcgaccgat catctggatc tgctgttaat ccaccgacca gacccgttaa
     8761 tggatgccga tgaagtggcg gacgcgttca aacatctgca tcagagcggc aaagtgcgtc
     8821 attttggcgt atcgaacttt acgcctgcgc aatttgccct gttgcaatca cgtctgccgt
     8881 ttacccttgc cactaatcag gtggaaatat ccccggtgca tcagccgtta ctgctggatg
     8941 gcacgctcga ccaactacaa caactgcgtg ttcgtccgat ggcgtggtcc tgccttggtg
     9001 gtggtcgtct gtttaatgat gattatttcc agccgctgcg tgatgaactg gctgtggtgg
     9061 cggaggagtt aaacgcgggc tcgattgaac aggtggttta cgcctgggta ttacgtttac
     9121 cgtcgcagcc gctgccaatt atcggctcag gtaaaattga gcgcgtacgg gcagctgtcg
     9181 aagcagaaac actgaaaatg acccgtcaac aatggtttcg tatccgtaaa gctgcactgg
     9241 ggtacgacgt accgtaagca cttttaggaa tagccgccgt tcaaaaatgt gtcactggtt
     9301 tacacttatt caggaatgca caatgaacgg aggtcctatg aaacgtttta gtctggccat
     9361 tctggcgctg gttgttgcga ccggcgcaca agctgccagt gaaaaagtcg agatgaacct
     9421 cgtcacgtcg caaggggttg ggcagtcaat tggtagcgtc accattactg aaactgataa
     9481 aggtctggag ttttcacccg atctgaaagc attacccccc ggtgaacatg gcttccatat
     9541 tcatgccaaa ggaagctgcc agccagccac caaagatggc aaagccagcg ccgcggaatc
     9601 cgcaggcggg catcttgatc cacaaaatac cggtaaaatt aaccatcgga aaggcgatga
     9661 tggcagccgt gtagcccgcc agttgaaatg catacgcgac gttattcgtg aagtgcgcac
     9721 aggcctaggt acaaaagcca agccacgccg acatgctcaa tagaaaaaac cacggctcat
     9781 tgagcgtatg tccggcaaga agcagtgccg caatggctcc cagtaaactg ccagcgatgc
     9841 gtccgaggct tttgctgatg acaccgccaa cggtgggaaa gctaaccact gcagccgagg
     9901 tcatcgccca atagggttca tccagattca aataataggc aaccgtcagc gccagacaca
     9961 tggcaatggt attgcgtaac gcataacgcc attgcgccag cgtggccctg aaccagcgca
    10021 aagcggacgg gaacgcgttg ggcgagacga acccagggga cgttaggttt cacatccgga
    10081 ataaggcttg agtcactttc aacgctctga tcataaatcg cccgaccgat actggaaacg
    10141 tgaccctgta acgttttgtt atcactatag agggtaattt gtgctggtgc accttcgcgg
    10201 atatggcgca acttggtttc ttcaaaataa ccaatgacgt aaaacgagtg gctgtcgaca
    10261 agggcaaaca gcggtttccc ggtgtcagcg taatcgccga tgcgggtagt gaggttagtc
    10321 acccatccac tcaccggggc gcgaatttcc gtttgcgcca gttgccattg tgtctgtttc
    10381 agcgtcgcct gtgcggcatt aacactggcc tgcatcgctt taacattgag gttagcggta
    10441 tcgagttctt cggcaaaaat aaaattttgt gagagatgac gacgacgatt cgcttcgtta
    10501 ttggcttttg cgaggtcaga ttgcgctttc gccagctgag cctgtgcgtt cagctcggcg
    10561 atctgaaagg gcgttttatc gatcgtgagc agaagttccc ccgcgttaac cagttggttg
    10621 tcttttatat tcagctcaac aatacgtccc gacacctgtg gggtgataga aacctgttct
    10681 gcgtgtattt tcccgtcgcg tgtccacgga gattgcatgt aataattcca cagccaccat
    10741 ccggcaagaa ccgcaactat cgctacaatg attgttgaga aatacttaat tgttttcatt
    10801 gacatagtta ccacgcaatc agtattgcca gagcaaggca aacgcaaatc gcaaacagcg
    10861 ataaatccat taacaaggga tgccagatgt caccggcgta gatccagcca cgaagcagac
    10921 ggtgtacgac aagccagatg acgaatccaa acgcgaatgc tttgaaaaac ggaggaaaat
    10981 agacggacgc accgaacacc agatcttgta agggcaatcc tgttgcattg agcataaact
    11041 tcacaaagta gattccttta cgaccgaata tattgcgtta gattaataaa tattctttaa
    11101 gtgcgaaaaa tttacgcgca atttctgaaa aatatgtctg acggtaacca aatgcagcaa
    11161 tacatttgtt ttagcaatac aattgctgca cactattcta aaagccgcat aatatcttag
    11221 caagctaatt ataaggagat gaaattggaa tcgccactag gttctgatct ggcacggttg
    11281 gtgcgcatat ggcgtgctct gatagaccat cgcctgaaac cgctggagtt aacacaaacc
    11341 cattgggtta cgttacacaa tatccatcag ttacctccag accagtcgca aattcaactg
    11401 gcaaaagcga ttggcatcga gcagccatca ctggtccgta ctctggacca actggaagaa
    11461 aaagggttaa tttcgcgtca aacttgtgcc agcgatcgtc gggctaaacg tattaaactg
    11521 acggaaaagg cagagccact gatcagcgaa atggaagctg ttattaacaa aacccgcgcg
    11581 gaaatattac atggcatctc cgcagaggaa ctggagcaac tgattacgct catcgcaaaa
    11641 cttgagcata atatcattga gttacaggcc aaagggtgaa atgaaggggg cgtgtggtca
    11701 gtaacctgac cacacgcaaa cttatttagc gcggagaaac ggtcacctga ctgccattgc
    11761 tggccagtac gacacgttgg cccggagaga aacgagtgtt gccttgtttc tgtaccacca
    11821 tgatggtatt accatcgtct ttacgaattt ccagctcgac accctgcgtt ttgttcattg
    11881 cactctgtac gccctgacca gctacgccac ctgcaacagc gcctgctgca gtagccagag
    11941 aacgcccggt tccgccacca acagtattcc ccaggaaacc accaagaaca gcaccgccaa
    12001 ttgcaccgat aacgttggaa tcatcaccgc cctgaatctg taccggacgt acgttaacga
    12061 tggtgccata gctgacattc tgtacttgtt tcgcttcaga agcggtataa acatcccctg
    12121 acagggtgtc gttattaaca caaccgacaa gagacagacc taccattgaa acaaccaata
    12181 cgcgtttaat cactgaaaaa tctcctgttc accatgaaac gccacgcaag catccctcat
    12241 ggtcaaagta tatggcatat gtgaatccat ggttacatgt tctaccaaat atgagcaaat
    12301 cataatgaaa gtaggatcaa ccaattataa acaaaacatt catgatgatt attgatttga
    12361 agataatgtt ttactaccaa cggtaaaaat cggtcattcc gcatggtatt cacgaaaagt
    12421 ttatgtttga gtggtgcgca aacgtcagat tgaaaaagga tgaactatga aatcgggccg
    12481 ctttattggc gttatgtcag gcaccagcct tgatggtgtt gatgttgtgt tggcgacaat
    12541 tgatgaacac cgggtcgcac agctggcaag cttgagctgg ccgatcccgg tgtctctgaa
    12601 acaggctgta ctggatattt gccagggcca gcagcttaca ctttcgcagt ttggacagct
    12661 tgatactcaa ctcgggcgac tttttgctga tgcggtcaat gccttgctta aggaacaaaa
    12721 cctgcaggcg agagatatag ttgcgatcgg ctgtcacggt caaaccgtct ggcatgaacc
    12781 gacgggcgtg gcaccacaca ctttacagat tggcgataac aatcaaattg tggcacgcac
    12841 cggaattacg gttgtcggtg attttcgccg tcgcgatatt gccttgggag gacaaggcgc
    12901 accgctggta cctgcgttcc atcatgcctt gctggctcac ccaaccgagc gacgaatggt
    12961 gctcaatatt ggcggcatcg ccaatctgtc actgctcatt cctgggcagc cggttggcgg
    13021 ctacgatacc ggtcctggta acatgctgat ggatgcctgg atctggcgtc aggcaggtaa
    13081 accttacgat aaagatgccg agtgggcacg ggcgggtaaa gttattctcc cactgctgca
    13141 aaatatgctc agcgacccgt atttctcgca acctgcaccg aaaagcaccg gacgcgaata
    13201 ctttaactac ggctggctgg agcgccattt acgccatttt ccgggtgttg atccccgtga
    13261 tgttcaggcc acactggcag aactcaccgc cgtgaccatt tctgaacaag ttttgttgag
    13321 cggtggctgc gaacgattga tggtatgtgg tggaggtagt cgtaatccgc tactcatggc
    13381 gcgtctggcg gtattactgc taggcacaga agtcaccacc accgatgccg ttggcattag
    13441 tggcgatgac atggaagcat tggctttcgc ctggcttgcc tggcggacgc tggcgggatt
    13501 accaggaaat ctgccttccg tcactggcgc aagccaggag acggtactgg gggctatttt
    13561 ccccgccaac tcgtgatatc agaattaact gaattttttc ttatcgtgtc tgccgttaca
    13621 cttggaagat taaggagggt atttgccctc cggaccagga aagtcttcgg gatatgacta
    13681 tgaaaaaact gttaattatc attttgccgg tccttctctc cggttgtagc gcctttaatc
    13741 aactggttga gcgaatgcaa accgatacgc tggaatacca gtgtgatgaa aaaccgttga
    13801 cggtcaaact gaataatccg cgccaggaag tcagttttgt ttacgataat caactactgc
    13861 atctcaaaca gggcctttca gcctctggcg cgcgttactc cgacgggatc tatgttttct
    13921 ggtcgaaagg tgaggaagcg actgtctata aacgcgaccg catcgtcttg aataactgtc
    13981 agttacaaaa tccacagcgt tgagattttt ccaggggcgg cgcacaatag cgtcacccac
    14041 tgacaatccg taaagaaaac catgtctgat aacgacgaat tgcagcaaat cgcgcatctg
    14101 cgccgtgaat acaccaaagg cgggttacgc cgccgcgatc ttcccgccga tccattaacc
    14161 ctttttgaac gttggctctc tcaggcttgt gaagccaaac tggcggaccc taccgcgatg
    14221 gtggtcgcta ccgtggatga acatggtcag ccttatcagc gcatcgtttt actcaaacat
    14281 tacgacgaaa aaggcatggt gttttacacc aacctcggta gccgtaaagc acatcaaatt
    14341 gaaaataatc cgcgcgttag cctgctgttc ccgtggcata cccttgaacg ccaggttatg
    14401 gtgatcggta aagcagaacg actttcgact ctcgaagtga tgaaatattt tcatagccgc
    14461 ccgcgtgata gccagattgg tgcatgggtt tcgaagcagt ccagtcgcat ttctgcccgc
    14521 ggtatccttg aaagtaaatt cctggagctg aagcagaagt ttcaacaggg cgaagtgcca
    14581 ttgccgagct tttggggcgg ttttcgcgtc agccttgaac agattgagtt ctggcagggc
    14641 ggtgagcatc gcctgcatga ccgctttttg taccagcgtg aaaatgatgc gtggaagatt
    14701 gatcgtcttg caccctgaaa agatgcaaaa atcttgcttt aatcgctggt actcctgatt
    14761 ctggcacttt attctatgtc tctttcgcat ctggcgaaaa gtcgtgtacc ggcaaaggtg
    14821 cagtcgttat atacatggag attttgatgg caagcagtaa cttgattaaa caattgcaag
    14881 agcgggggct ggtagcccag gtgacggacg aggaagcgtt agcagagcga ctggcgcaag
    14941 gcccgatcgc gctctattgc ggcttcgatc ctaccgctga cagcttgcat ttggggcatc
    15001 ttgttccatt gttatgcctg aaacgcttcc agcaggcggg ccacaagccg gttgcgctgg
    15061 taggcggcgc gacgggtctg attggcgacc cgagcttcaa agctgccgag cgtaagctga
    15121 acaccgaaga aactgttcag gagtgggtgg acaaaatccg taagcaggtt gccccgttcc
    15181 tcgatttcga ctgtggagaa aactctgcta tcgcggcgaa caactatgac tggttcggca
    15241 atatgaatgt gctgaccttc ctgcgcgata ttggcaaaca cttctccgtt aaccagatga
    15301 tcaacaaaga agcggttaag cagcgtctca accgtgaaga tcaggggatt tcgttcactg
    15361 agttttccta caacctgttg cagggttatg acttcgcctg tctgaacaaa cagtacgatg
    15421 tggtgctgca aattggtggt tctgaccagt ggggtaacat cacttctggt atcgacctga
    15481 cccgtcgtct gcatcagaat caggtgtttg gcctgaccgt tccgctgatc accaaagcag
    15541 acggcaccaa atttggtaaa actgaaggcg gcgcagtctg gttggatccg aagaaaacca
    15601 gcccgtacaa attctaccag ttctggatca acactgcgga tgccgacgtt taccgcttcc
    15661 tgaagttctt cacctttatg agcattgaag agatcaacgc cctagaagaa gaagataaaa
    15721 acagcggtaa agcaccgcgc gcccagtatg tactggcgga gcaggtgact cgtctggttc
    15781 acggtgaaga aggtttacag gcggcaaaac gtattaccga atgcctgttc agcggttctt
    15841 tgagtgcgct gagtgaagcg gacttcgaac agctggcgca ggacggcgta ccgatggttg
    15901 agatggaaaa gggcgcagac ctgatgcagg cgctggtcga ttctgaactg caaccttccc
    15961 gtggtcaggc gcgtaaaact atcgcctcca atgccatcac cattaacggt gaaaaacagt
    16021 ccgatcctga atacttcttt aaagaagaag atcgtctgtt tggtcgtttt accttactgc
    16081 gtcgcggcaa aaagaattac tgtctgattt gctggaaata atgcattaag tggaaagggg
    16141 gagtgagaaa tcactccccc tggtttttat acagggaaca tgatgaaaaa tattctcgct
    16201 atccagtctc acgttgttta tggtcatgcg ggtaacagtg cggcagagtt tccgatgcgc
    16261 cgcctgggcg cgaacgtctg gccgctgaac accgttcaat tttctaatca cacccaatac
    16321 ggcaaatgga ctggctgcgt gatgccgccc agccatttaa ccgaaattgt gcaaggcatt
    16381 gccattgccg ccattgataa attacacacc tgtgatgccg tattaagtgg ctatctggga
    16441 tcggcggagc agggtgaaca tatcctcggt atcgtccgtc aggtgaaagc agcgaatccg
    16501 caggcgaaat atttttgcga tccggtaatg ggtcatccgg aaaaaggctg tatcgttgca
    16561 ccgggtgtcg cagagtttca tgtacggcac ggtttgcctg ccagcgatat cattgcgccg
    16621 aatctggttg agctggaaat actctgtgag catgcggtaa ataacgtcga agaagcggtt
    16681 ctggcagcgc gcgaactcat tgagcaaggg ccacaaattg tgttggttaa acacctggcg
    16741 cgagctggct acagccgtga ccgttttgaa atgctgctgg tcaccgccga tgaagcctgg
    16801 catatcagcc gtccactggt ggattttggt atgcgccagc cggtaggtgt tggtgatgtg
    16861 acgagcggtt tactgctggt gaaactgctt cagggggcaa cgctgcagga ggccctggaa
    16921 catgtgaccg ctgcagtcta cgaaatcatg gtgaccacca aagcaatgca ggaatatgag
    16981 ctgcaagtgg tggctgctca ggatcgtatt gccaaaccag aacattactt cagcgcaaca
    17041 aagctctgaa atatctcatt tcggcccaca acgacctgtg ggccgatggc tattacttta
    17101 agccttccgc tgacagcgcg tcttgtactt ccggacgttc agccatacgt tgcataaatg
    17161 ccgcaatgtg ctctaaccct tccagattca gtttcaccgc gtatgcccag cgcagaaccg
    17221 taaacagata ggcatcagca attgtaaatc tttgcccgca gatccagtgc tcatccttca
    17281 gtgcctcgtt cacatattgc agcttcttat ccagctgcgc gcgaactgtc ggtttgtact
    17341 cttccggtgt atctgggcga aacagaggtg tgaaaccttt atgcagctcg gtggcgatgt
    17401 aattcagcca ttcgatggtt ttatagcggg aaatactgtt taccggtgcc agcaactggc
    17461 ggtcggggac gctgtcggca agatactgca taatcgcaac gccttccgtc agcaaagtac
    17521 catcgtccag cagcaatgca ggcacctgcc ccttagggtt aacggaaaag taatcgtcac
    17581 cgttttcgag acgttttttc attaaatcca cactgacgag cgtaaaatcc tttccgctct
    17641 cacgcagggt aatatgggaa gcgagagagc aggcgcccgg tttgtagaac aatttcatcg
    17701 gtaactcctt ttgtctgtgg tttaaagtat ggtagtgcgt tgctgaacaa aaaaaaagcc
    17761 gctaacgaaa agttagcggc ttgtaaaaga gtttccctga attttacgct acagtagctt
    17821 tcgccgcttt gtctgcagcg tcatcctgcg tcatgcggtg cagtttaggc gcggtcagca
    17881 gcatcagtac tgcaataacg gcagtagcga caccaatctg caagaatacg cgaccataga
    17941 cttccagtga catcagcggg tcggtaacgt tatccggcac agccatcata cccgcaacat
    18001 aaccaccaat caggtttgca ccggcagtgg tcaggaacca gctacccata atgaagccca
    18061 tcagacgctg cggaacgagt tgtgcaacca ttgccagacc cagaccagag atcatcagtt
    18121 ccccgatgct ctgcaggcca tagcttgcga ccagccagct gacagacacg atacctgcgt
    18181 cagacgcgaa tttcgcaccc agcggcagaa tcaggaacgc accagaacac atcaccatgc
    18241 cgattgcaaa cttggttggc atcggcaggg tatcacccat cttgttatag atagcggcca
    18301 gaatcggact accgatgatg atccagaacg ggttcagtgc ctgatactgc tcaggttcta
    18361 cggccagacc cagaatggag tgctcaacgt tacgaatcgc aaagaagttc agtgacgttg
    18421 gcatctggct gtacagcacg aagaagatga ttgcttcgag catcaggatg aaggcaacga
    18481 tcattttacg acgcgcagca cctttcatcg cgaaagcttc tttaccgaag ataaccacaa
    18541 taccgaaggc aacaacgccc agcgccatac gcgcaacttc ctgattgtgc agtagccagg
    18601 tggcaacagc gatcagcgcc acaacaccaa taatggtcag caacaggtta cggtagttga
    18661 ctggttcaaa gtctggtttt gaaccgtact gtttaaccca gcgttggcag aaggcgaagt
    18721 taacgatagt gatcagcagg cctacaacgc tcaacgcaaa cgcaacactc cagccgtatt
    18781 tcgcggccag ccacggggtg gctatcatag agaagaaaga gccgatgttg acggacatgt
    18841 agtacatggt gaatgcaccg tccagacgtg ggtcgttttt ctcatagcat gtagaaagca
    18901 gagaagacgg gttagcttta aacaggccgt taccgaccgc aatagccgcc atacccatat
    18961 aaacaatacc ggcgtcgtga ccagaccagg caaccagagc ataaccaatc gccagcacaa
    19021 tagcgccgag cataattacg cgtttagtac ccagtacctt gtcacctaac cagccgccga
    19081 tagcgaccag accataaacc agggcactaa aggaagagaa aagggtgatt gaatccgctt
    19141 cagacatacc cagttgttta accaggtaaa cagccataat tccttgtagg ccgtaataac
    19201 caaaacgttc ccataactca atcgagaaga tgagatagaa cgccttcggt tgtttgaaag
    19261 cgttcaaact gacgctttca gttggttttt ggtttgcagt ggacactttt acctcttttt
    19321 ttacatccca tattaacggg ggtgctttct cgcgttatgt ctaaaaaaca tacgcgcaat
    19381 tgttatagtg ggaggggaaa cggcaggtaa tgttcactat cttgcacttt cagacaagtc
    19441 ctttgtaata atctgttaca tataactgag gcggggaatt caaccattcc gggaaatgtt
    19501 atccagcgtt aaatttttca aaaaacgtaa cggcagattt tttcactgca ttgagagggg
    19561 gatgatggct ttacggagat atgttctgct atttagcgtt tttcctagtg agatagtcca
    19621 gtttctgaaa aatagccagt gtaatgtttt gcaggtcaat atttgttgcg tgtgtaaatt
    19681 taaatcattt taacctgatt cttgcaatgt ttttacattg ccacagcatc gtgatcttga
    19741 tcacgaatga atagaaaatt tcgctattaa aaaaacaatt aacctgcatt tttggttatc
    19801 cgcaaacgga ttacctgatg ataacgctaa gcaatggcat cattgacgtt aaaaagatgt
    19861 tttaaaagag gataaagaaa ggttatcaat ggggtaatcg gtgttacccc ttttcttcag
    19921 atgtcaactt tctctttgta ttcacaaaga tcttcaataa tacaagagcc acagcggggc
    19981 ttgcgggcaa tgcaggtata acgcccgtgc aggatcaacc aatggtggca gtcgacttta
    20041 aactctgccg gaaccacttt cagtagcttt tcttctacct gttcgacgtt tttccccggc
    20101 gcaaattgag tacgattaca aacgcggaaa atgtgcgtgt cgacagcaat agtcggccag
    20161 ccgaatgcag tgtttaatac gacgttggct gttttacgac ctacgccggg cagggcttca
    20221 agcgcagcac gatcttccgg aacctcgcca ttatgttgtt ccagtaatat acggcaggtt
    20281 ttgatgatat tttctgcttt gctgttataa aggccaatcg ttttgatata ggttttcacc
    20341 ccttcaacgc ccagttcaag catcgctgca ggcgtattcg ccaccgggta gagtttcgcc
    20401 gtcgccttat taacactgat atcggtcgcc tgagcggaaa gcagtacggc aatcagcaat
    20461 tcaaaaggcg aactgaaatt aagctcggtg gtgggatgag gattgttctc acgcaggcga
    20521 gtgaggatct ccagacgttt tgctttgttc atcagacatt ccctgtttca ccgtttggca
    20581 atgcacgttc ggcagctgct tcagcacggc gctttttcat tctttcatca atcaggtatt
    20641 ttcctgccag catcagtccc aagccaataa atgcacctgg tggcagcatc gccagcagga
    20701 aaggggagtc ggtgtggaaa atctccacgc gtaatacttt tgcccagcta cctaacagcg
    20761 catctgcacc gtcaaacaac gtgccattgc cgataatttc gcgtagtgaa cccagcacga
    20821 acatggcgca ggttgcgccc gtaccaattg aaaagccgtc cagtgccgaa agcgccggac
    20881 cttttttggc ggcgaaggct tcagcgcggc ccacaacgat acagttagtg acaatcagcg
    20941 gaataaaaat ccctaatgat tgatacaggc caaaggcgta ggcgttgatc agcatctgta
    21001 cagcgctgac caccgaggcg atgatcatca cgtaaatggg aatgcggatc tcggctggcg
    21061 tccagtgacg cagcgtcgaa atggtcaggt tggtcagcgt cagtaccagc gtagtcgcaa
    21121 gtcctaaacc cagagcgtta gtggcagtgg acgtgaccgc caatagcgga caaaggccga
    21181 gcagttggac cagtgcagag ttgtttttcc acaacccctg aacaataacg tctttaattt
    21241 cgctcacgct ttattctcca caggcaggaa gttgagaaag ttgtgccggt aacgtctgag
    21301 cgtacaatcc ggcgcgtttt accgcattaa ccaccgcgcg gggagtaatc gtcgcgccgg
    21361 tgaactggtc gaaatcacca ccatctttct tcaccgccca gtgcgcatca tccgcaccac
    21421 tgattttttt tcccgcaaaa tgggtgagcc agtcagaaag gcgcaattcg attttatcgc
    21481 caagccctgg cgtttcgtgg tgctctgtca cgcgcgtgcc aagtaccgtg ccattaaaat
    21541 cggctcccac cagcagctga atcgcaccgg aatagccatc tggcgcggtt gcttccagaa
    21601 cggcggctac cggtttgtca tcctgtttgg cgatgtaaac ccgatgctca cctttaccta
    21661 actctggcgc agttaccaga tagcaactct gtgcgagcgc attgttatag cgttcggctg
    21721 gcagcacctg accaaataac gccttttgtt gcagactggc ctgttcagca attgtcgttt
    21781 tggtcatctg gttgatggcc gcagttaacc ctgtagaacc cgctgcaaat agtgccagtg
    21841 taatgccgtg ttttcggata gttttcagca tggtttaccc tttgcgatgg ccgtagacgc
    21901 gcggacgcgt gtagtaatcg atcagaggaa ccgtgatgtt cgccagcagg acggcaaaag
    21961 ccacgccgtc aggatagccg ccgaaactgc ggatcaacca gactaataag cccgccagcg
    22021 cgccgaacat cagccgacca cgattggtcg tagaagcagt aactgggtca gtcaaaataa
    22081 agaatgcgcc gagcatagtc gcaccagaca gcagatgaat ttgcggtgct gccagtgttt
    22141 ctggtgagaa caaccagccc aacgttgcgc ataacgccag cgttactaag aagctgaggg
    22201 gaatatgcca gcgaatcgct ttctgccata gcaaccacac gccaccagcc agccaggcga
    22261 gatttaccca ttgccaacca gcgcccgcca gaataccgct gtagatcgga tattgcataa
    22321 tctgttcaac cgaatgacct gcgcggacag aggttttgaa tgtatccagc ggtgtcgcct
    22381 gactaatgcc atcaatacct aagcgtagcg tattcatatc accaccactg gcggtatgac
    22441 cgctgaaaat aacctgtatg gcgtcgataa aaccagggat gttgaccgca atttcatgtg
    22501 gcggtaacca gctggtcatc tgcaccggga aggagatcag taagaccaca taaccaatca
    22561 ttgccggatt aaacgggttt tgccccagac cgccatacaa ctgtttagcg atgatcaccg
    22621 caaacaccgt acccagtacg accatccacc atggcgctag ggggggaata cttaccgcca
    22681 gcaataagcc tgtcagcaat gctgagttat ctttcaacgt tgcggctacc gactgcttgc
    22741 gtagtttgag tacgagagct tcggctaaca gagcactgac cgacgccaac aggatctgaa
    22801 cgagagtacc ccaaccaaaa aaccacagtt gcgctgcgat tcctggcacg gctgcgagca
    22861 acaccaatag cataatgcgc gatgtctggc gctggttatg ggtataaggg gagctagcta
    22921 ttctgaatac catttagtcc tcgtttacaa ccttttgctg ggcggctttt ttggcctgaa
    22981 cgcgggcaat agccgcggca actgcggctt tgcgcggatc gacctgttct tctggttccg
    23041 cattagcctg ttgctgttcc agcttgcgtg ctttggcgcg ggcaatagcg gcttcgacgg
    23101 cagctttgcg cggatcaacc tgttcttctg gctccgcatt ggcctgttgc tgttccagct
    23161 tgcgtgcttt ggcgcgggca atagcggctt cgacggcggc tttgcgcgga tcgacctgtt
    23221 cttctggttc cgcattagcc tgttgctgtt ccagcttgcg cgctttggca cgggcaatag
    23281 cggcttcgac ggcggctttg cgcggatcga cctgttcttc tggttccgca ttagcctgtt
    23341 gctgttccag cttgcgcgct ttggagcggg cgatagctgc ttcaacggca gttttacgtg
    23401 gttcagcaac ggttgctgcg tcgttagttt gctccagttc tgcctgtttc gctctggctt
    23461 gcgctttacg ggcttcccgt gctgcaataa ttgcactgtt atccgggcgt tcgcccgctt
    23521 taatcacaat aggctgtgta gcctgggcct gtttctcttt cacccgcgcc agagcggcag
    23581 caatcgcatc tttatctttg gctgcaggtt gaacggctgc gctcttatgt cgttcaaggc
    23641 gagccgcttt ttcgcgctcc agacgagcct ggcgcgcttc gaaacgcgct ttggcttctg
    23701 cggcgcgctt ttcttcctga cgaatagccg caatttcagc tttttcctga cggaaatatt
    23761 gcaccaaggg aatattgctc gggcaaaccc acgcgcaagc cccacattca atgcagtcgg
    23821 caatgttatg cgtggtagct ttatcgtgtt gctgaccttt gctgaaccag tacaactgtt
    23881 gcggcaaaag atccacaggg caggcgtcag cacaggcgct acaccggatg cagctttgtt
    23941 cttcctgtgg ttcgccaagt tcattggcag agggagccaa cagacagttg gtaatcttta
    24001 cgaccgggac atccagccat ggcaaggtaa agcccattag cgggccaccc ataatcacca
    24061 tttgatcggc agaggggcag aatccggcat cattcaataa atgacgcact ggcgttccca
    24121 gccgtgccca gacgttgccc gggcgagcga ttgcttcgcc agtcagggtt acaacacgct
    24181 cggtaatcgg ctcgccatca ataacggcac gtttcactgc ataagcagtg ccgacgtttt
    24241 gcattaatac gccgatatcg gatgaacgcc cgccatgtgg aacctgcttc ccggtcagaa
    24301 tgtaggttaa ttgtttagca ccgccagaag gatatttggt tggaatcacc cgcagagaaa
    24361 tatcgttaga gtccgccagc accgcgcgca gcatggaaat cgcctgcggt ttgttatctt
    24421 caatgccgat aagaatttcg cgtggctgca gaatatgcgc aagaatgcga ataccctcta
    24481 cgacctgagc cgcgcaatcc tgcatcaaac ggtcatcggc ggtaatgtac ggctcgcact
    24541 cagcagcgtt gataatcaac gtttcaatct tatctccacc accctgcaat ttaacgcctg
    24601 tcgggaatcc ggcaccgccc agcccggcaa caccaaactg atgtatgcgc tcgattaact
    24661 cttcgcgact gcgagagcga taatcgaccc agccgtcgcg cgggatccag cagtcttcac
    24721 catcggcatc aataatcacg cttaattcag ctaaagctga aggatgagcc gtagagtggg
    24781 gcgcaatagc cgtaacggta cccgaggtgg gcgcgtgaac aggcagcatt ttgccgcgac
    24841 cacgggtaag cggctggccg cgcaatactt tatcgccgac gctaacgcac aactcacctt
    24901 cagcgccaat atgctgtttc agtggaataa caaaacgctg cgccagtggt acctggcgca
    24961 ggggggtacc gttggactgg gttttcatct ccggtggatg gatgccgccg ttgaaatccc
    25021 agattttatt ttttctgaat gcagagaata acttaagcat ggtgttccac gggaatgata
    25081 cgcacgggaa tggtgttcag atcccatttc caggagtcag gtgtttctgc gaccggttgc
    25141 aacgagatgc agtgcgtcgg gcacggatca acacataaat tgcagcccgt acagagatca
    25201 ctcattaccg tatgcatggc acgggtagcg ccaacgatgg cgtctaccgg acaagcctga
    25261 atacatttag tgcagccaat acagttattt tcatcaataa ccgccaccat ccgcgcaggc
    25321 gttagctctt gcgcttcgcc atccagcggc tgcggctcga cattaagcaa ctcggcaatt
    25381 tttagcatca cagcttcgcc acctggggcg caacggttga ttttttcacc gttacagctg
    25441 atggcttccg cgtaggggcg acagccggga taaccgcact gaccacactg gctctgcggt
    25501 aagatttcgt caattttctc aacgaccgga tcgtcttcca ccgcaaaacg gcgggaggca
    25561 taacccagaa tggcgccaaa cgccaggccc agcaggctca cggcggcaac ggcaatccag
    25621 atagcattca ttacaacttc accaaaccac taaagcccat aaaggccaga gacataagac
    25681 ctgcggtaat taacgcaatg gcattaccgc gaaaaggtgc cgggacatca gccacagcaa
    25741 ggcgttcgcg gatggtggcg aagagcacca tcaccagcga gaaaccgaca gcggcggaaa
    25801 aaccgtacag cgccgactgc aagaaattgt gcccgagatt gatattcagc aacgccacgc
    25861 cgagcactgc acagttggtg gtgataagcg gcaaaaaaat ccccagcaga cggtaaagca
    25921 ccgggctggt tttgcgcacc accatctcgg tgaactgcac gaccacagca atcaccagaa
    25981 taaatgccag ggtgcgtagg taaatcagat taagtgggat caaaatccac gtatcgataa
    26041 gccaggcgca aatagacgtc agcgtcatca caaacgttgt tgccagcccc atgcccatcg
    26101 cggtttccag ttttttggaa acccccataa acggacagag accgagaaac ttgaccagta
    26161 caaagttatt gaccagtaca gttccgacaa acagtaacag gtagtcagtc atttttcggc
    26221 ctgaaataaa aaagccgcct attatccgtt aatccagagc aggcgacaac aggttaactg
    26281 taaggttatt acggattcac aaacgtcgct tttacccgcg acgagcgttt gaaatagggg
    26341 acgatcaatg cggttgccaa caaagtaaac agcaactggc gaacggctat gccaacttcc
    26401 acgggtgaaa aagcgaatgc ttttactgcc agcaatacgg aaataagcag ccagataata
    26461 tagtgtttag gaacacaacg ccgacgttta aagaaagcga tggtcaacca cagtgtgtaa
    26521 taccacatgg cgatagcggt aataaaagaa acgccccata agatttgtgt cgtcagtgct
    26581 tgtccgccga gcgtttgaaa tgtttgcgga gaagataacg cagcggtata cagcaacaac
    26641 gccagcgtcg tactcaacag agcgactaaa agccaggcca acgggccaag caaccagcct
    26701 ccaatacgtt gtggcgttgt ggtggtcata ggttctccag aatagcgacg caaacaggca
    26761 aatctaattt taggggcgtt attataaggc ttttataacc gatcgctact ttctttattg
    26821 gacatagtgc cagacggact tcggtacctg gccgaggtca aacaaacgcc cgccagaaac
    26881 cagctctgcg cgacggtgat cggcagcacg atacatattg atcagttcct gatcgtccgt
    26941 cagggtgtaa ttaagatggt cgtagagttt ttccagactt tcgagtgaac tgattttgcg
    27001 aaattttaat aagtagtcct gaacagtcat aaaaggtcca taaaataatt aataccaaag
    27061 gggtaggtat tattagtgat gataaaattt caccaaaagt gaacgcttgt gttattacaa
    27121 taataagaat gcgctgatgg caaatattca gggatgaaaa actgctctga aaagagtaac
    27181 gcgctttact atccctgaca atcaccaaca acatcgaaca agataataaa ttcctggttt
    27241 aatatccgac aagtgaaaac atgcacccgg acgggcagca tgtcgctcca caagtgcaga
    27301 gcttacttgt gttgtaccga agcactctgt tcaggtggct gatagttgtc aatgtgactc
    27361 gccacgccaa gaagaatgac tgaaacgaca agaacgatcc aacctgttaa ttcaataaga
    27421 cgattcatta cagcccacat ctctcttgat tgatccatta acttcagggg gtaaatgtta
    27481 cttagcaata atagctccag caagattttt actgaggttt ttgcgatatt tagcttttgt
    27541 cgttggaaaa ttcgctattt ttttgactta agttaaacaa catcccttat tgctggcagg
    27601 ttattaaact gttgagcgtg ggtaaaggat agtgtcaaat agccatcata cttcaatgag
    27661 aggcaatgac atgagcgaca acatccgtgt tgggttgatt gggtatggtt atgcgagcaa
    27721 aaccttccat gcgcccctga ttgcgggcac gcccgggctg gaactggcgg taatctccag
    27781 cagtgatgaa acaaaagtaa aagccgactg gccaacggtt acggttgtct ctgagccgaa
    27841 gcatctgttt aacgatccca acatagacct gattgtcatt cctacaccca acgataccca
    27901 tttcccgtta gccaaagcgg cgcttgaggc gggtaaacat gtggtcgttg ataaaccctt
    27961 taccgtgaca ctgtcacaag cgcgagagct ggatgcgctg gcaaaaagcc tggggcgtgt
    28021 gctgtctgta ttccataacc gtcgttggga tagcgatttc ttgacgctaa aaggtttgct
    28081 cgcggaaggc gtgctgggtg aagttgctta ctttgagtct cattttgacc gcttccgtcc
    28141 gcaggtgcgc gatcgttggc gtgaacaggg cggcccaggc agcggtatct ggtacgattt
    28201 agcaccgcat cttcttgatc aggccattac gctatttggt ttaccggtca gcatgacggt
    28261 agatttggca cagttacggc ccggagcgca gtcgaccgat tatttccacg ccatcttgtc
    28321 ctatccacag cggcgagtca ttttacacgg taccatgctg gcagccgctg agtcagcacg
    28381 gtatatcgtg catggatccc gaggcagtta tgtgaaatat ggcctcgatc cacaggaaga
    28441 acgtctgaaa aatggcgagc gtctgccgca ggaagactgg ggctacgata tgcgtgatgg
    28501 cgtacttacc cgcgtggaag gtgaggaacg tgtcgaagaa acgctgttga cggtgcctgg
    28561 gaattatccg gcttactatg cggcgattcg tgatgcgtta aatggcgatg gtgaaaatcc
    28621 ggttccggca agccaggcaa tccaggtaat ggagttgatt gagctgggca tcgaatccgc
    28681 caaacatcgc gcgacgttgt gccttgcgtg aaaaatggat ggtgcaattt gcaccatcca
    28741 tttttgttac ttcgcggcga ctttttctcg cagtgcgcgt ttttcctctg cgctgaggaa
    28801 agccatttcc agaccattaa tctgtgcctg gcggatttgc tcacgggata acccagcagc
    28861 tggcgcggca acggtatatt cgtgaatgat atccactccc tgtacgccgg gatcgtcagt
    28921 gttaatgctg gcacgaatgc catgctcaag gaacgttttc agcggatgtg cagccagctc
    28981 tgctacggtg ctggtctgaa tattggaggt cagacaggat tcaataccaa tttgttgctc
    29041 ggcgagaaaa tccatcagcg cccgatcttc aatggctttt acgccatgtc caatacgctc
    29101 cgcacccagt tcacgaatcg cctgccagat gctttccggc ccggcagctt cgcctgcatg
    29161 gacggtaata tgccagcccg catcacgcgc gcggttgaag tgagaaagga acagacttcc
    29221 cgggaatcca agttcatcac cagctaaatc aagtgcggta atctggtcac ggtgggctaa
    29281 aaaggcctcc agctcttgct gacaggcggc ttcgccgaag gttcggctca taatgccgat
    29341 aagcttcgcc tgcacaccaa aggtgcggca accttcacgt acgccatcga tcaccgcttc
    29401 gacaacaccc gctacaggca gcttatgtgc cattgccatg tagcctggtg aaaaacgcag
    29461 ctcgacatag cgcaggccgt tacgggctgc atcttcaatg ttttcaaatg ccacgcggcg
    29521 gcaggcatca agagaggcga gaactttaac gccccagtca agtttggtca gaaagctcac
    29581 cagatcgggt tcgttggcaa tgacctgaac gtggggaatc agtgtttcaa gggattgtgc
    29641 aggaagcgag atattatact ggcggccaag ttcaagaatg gtctggggac gaatgttgcc
    29701 atcaaggtgg cgatggatat cagttaatgg cagggtggta tcaatcatgg tcgcactctt
    29761 tttctggata taaaagtgcg caccattata aaaacaaagc agggtaaaaa tccacgattg
    29821 cgcaacgatt tttacgttgc gcaattgggg ttagcgaaca gcgcggatgg cgtttattaa
    29881 tccagccaca cctttttcca gtttcgaacg tgggcagccg gcattgagac ggacaaaacc
    29941 acgaccttct tcaccatagg tataccccgg catgatcgcg actttttctt gctcgataag
    30001 cgctttttgc aacgcgttgt cgtcaatatt caacggacgt agatcaagcc aggccagata
    30061 agtggattgc gggatctgcc agttgagttc aggaaacgcg gcgttcattt tatctgagat
    30121 atacgtcagg ttatctttca gatagacgcg taaggcatcc agccacggtg caccttgctg
    30181 ataggcggcg atatgggcag ttaacgccag taccgaaggg gaagaaagcc catcacgtcc
    30241 tttcagtgcc gataaatagg catcacggct gctgctattt tctataatcc cgtaagcacc
    30301 ggtcagcgcg ggaatattga aacttttcga tcccgacgtc agcaacgccc agtctccccg
    30361 cgccacatta ctccagggaa tatgcggctg ctcgccccag accatatcca tatggatttc
    30421 atcggaaata acccgcacat catgacgctc gcacaggtca gccatgatct ccagctcatc
    30481 gcacgtccac actttcccgg taggattctg tgggttacac aggagcataa ttttacattc
    30541 tggtttcgcc aaaacggctt ccagcttgcc catatcgcaa aaccagccgt cagcctgctt
    30601 ctctaaagca acgggcatta ctgtgcgctg gttaccttca atggccttgt aaaatgcgtc
    30661 ataggcgggc gtgtggatca ccacgccttc acctgtttca gaccactgac gaatcagttc
    30721 tgaaaccata tagatgacag aagggccata caccaccgtc tgcgtatcaa ttgcggtgta
    30781 atgctgggtg gaaaaccagt gggcaatagc cgcgagaaac tcatcgtttt tccagcggct
    30841 gtagccaaat acgccgtgca tcaggcgctg attcagcgcc tcgataatgc agggggcagt
    30901 ggcaaaatcc atgtctgaaa tcgtgaacgg taacaggtca gcagtgccga aacggtcagc
    30961 gacataatcc cactgtgtac accatgtgcc atgacgatcc acgacctttg aaaaatcgaa
    31021 catatcttat ccttatgcct ggacagtatg catcagaccg gccatttcat cttttactga
    31081 ctgaacttgt ggcccgataa caacctgcag gttatgttga ttaagttgta ctacgccaat
    31141 tgcccgattg tccttcagtg cttgcacatt aacaagcgac atatcttcca cagacaaacg
    31201 cagacgggta atgcagttat cgaggctgac aatattgtcg gcaccgccta atgcttcgag
    31261 gattgcagga acgttgtaac ctgatttacc cggcgcaccg gcaacggctt tttcgattga
    31321 gctggcaact tcgctatcgc gccccggggt tttcagattg aagcgggtga tagcgaaacg
    31381 gaagatgacg tagtaaacga caaaccagat tgccgccacc actggcacca tgtaccactt
    31441 ggttgacaga ccatgcaaaa taccgaacac cacgaagtcg atgatattgc cgtcggtatt
    31501 accgatggtg acgccgagca cagacatgac ggtgaagccg aggccggtta acagcgcgtg
    31561 gatgacatac agaactggcg ctacgaacag gaacaggaat tccagcggtt cggtagtgcc
    31621 gccaacgacg caggcgatca ggccagaaat cagcagacct ttaattttat ggcgattttc
    31681 cgggcgcgcg cagtgataca tagctaacgc tgcacctggc agaccgccga gaaacgcagg
    31741 cattttacct tgcgaaagga aacgcgtgac gctttcagaa aaaccgtgag tggtcgggca
    31801 actcaattgc gcctggaaga tggtcagtgc gccgctgacg gtttgaccgc agacctcctg
    31861 cgtgccgcct gcgtcggtaa agcgaattaa tgccaccaga atgtgatgca gaccaaacgg
    31921 taacagcaga cgttcaccgg taccaaacag catcggtccg aaatcacccg cgctgtttat
    31981 catatggccc aagccgctaa tacccatggc gaaaatcggc cagactaatg gaatcaccag
    32041 gccgacaagg cccatcacca gcgaggagat aattggtacg aagcgcgtac cgccgaagaa
    32101 tgccagcgca tccggcaggc ggatattatg gaaacgctca tgcagcatcc agacgataat
    32161 accggcgatc accgcaccga ggatcccggt atcgatcgac tggatcccaa gaatgctctg
    32221 gatgttattg gctttcagaa cggcggcatc tgtggttggc agaatgcctt tattggtcaa
    32281 ccagaagttt accgcgaggt tcattaccgc ataaccgacg aagccagcga atgccgctac
    32341 gcctttattt tcgcgtgcca ggcccagcgg gatggcgata cagaacatga caggcaggaa
    32401 actaaaagca aacgagccaa tcttactcat ccaggtaaag atagcctgca acacggggtt
    32461 gcccaggacc gggatcaggg ttatgacatc atggctgcta agagaactac caatgccgag
    32521 cataatgccg cagaacgaca ataatgccac gggtaacatg aaggttttgc ctaactgctg
    32581 gaagaactcc cacagcgtga ctttcggtgc tgttttcgcc gtcataaaac gactcctcgt
    32641 agagaacaat ctggattcag taaattgcgc gagaagataa aacgttttat caaattttag
    32701 tgagtcataa atcacattac gcaacgataa tagcgggtat aagataaata aaaggtaaaa
    32761 cgttttatct gtcacataat cagggagtag gtcatctgca tggctaccgc caaaaaaata
    32821 accattcatg atgttgcgct ggctgcgggc gtgtcggtaa gtaccgtttc gctggtgctt
    32881 agtggcaaag ggcgaatctc taccgccaca ggagaacgcg ttaacgccgc cattgaagag
    32941 ctgggatttg tgcgcaatcg ccaggcgtcg gcgctgcgcg gcgggcaaag cggcgtcatt
    33001 ggtttgatcg tccgtgattt atctgcgccg ttttacgccg aattgacggc cggattgacg
    33061 gaagctctgg aagcgcaggg acggatggtt tttttgcttc acggcggtaa agacggcgag
    33121 cagctggcac agcggttttc actgttactg aatcagggtg tcgatggtgt ggtaattgcc
    33181 ggggctgcag gaagcagcga tgacctgcgg cggatggcag aagaaaaagc tatcccggtg
    33241 attttcgctt cccgtgccag ttatcttgat gatgttgata cggttcgccc ggacaacatg
    33301 caggctgcac agttgttgac ggagcatctc attcgcaatg ggcatcagcg gatcgcctgg
    33361 ctgggagggc aaagttcctc attaacccgt gcagaacggg tggggggcta ttgtgcaact
    33421 ctactaaaat ttggcctgcc gtttcacagc gattgggtgt tggagtgcac ttccagccag
    33481 aagcaagccg cggaagctat cacggcgctt ttacgtcata acccgaccat cagtgccgtg
    33541 gtttgctata acgaaactat tgcgatgggg gcatggtttg gtttgctgaa agcagggcgg
    33601 caaagcgggg aaagcggagt cgatcgttac tttgagcaac aggtttctct ggcggcattt
    33661 accgatgcga caccaaccac acttgatgat atacctgtta cctgggccag cacgccagcg
    33721 cgggaacttg gtatcacact tgcggatcgc atgatgcaaa aaatcaccca tgaagagacg
    33781 cattcacgca atcttattat tcccgcccgg ctcattgcgg cgaaataatc ctctctttat
    33841 ctgctatact tggtagtgtc ctttcctcaa ggttaatgat aaaaaatgtg gcacacaggc
    33901 ggtgcctttt atttttactg aagttcacag gaggtttatg tgtttaattc tgacaacctg
    33961 agactcgacg gaaaatgcgc catcatcaca ggtgcgggtg caggtattgg taaagaaatc
    34021 gccattacat tcgcgacagc tggcgcatct gtggtggtca gtgatattaa cgccgacgca
    34081 gctaaccatg ttgtagacga aattcaacaa ctgggtggtc aggcattttc ctggcgttgt
    34141 gatattactt ccgaacagga actctctgca ctggcagact ttgccgtcag taaactgggt
    34201 aaagttgata tcctggttaa caacgtcggt ggcggtggtc ctaaaccgtt tgatatgcca
    34261 atggcagatt ttcgccgtgc ttatgaactg aatgtgtttt cttttttcca tctgtcacaa
    34321 cttgttgcgc cagaaatgga aaaaaatggc ggtggcgtta ttctgaccat cacttctatg
    34381 gcggcagaaa ataaaaatat aaacatgact tcctatgcat catctaaagc tgcggccagt
    34441 catctggtca gaaatatggc atttgacctg ggtgaaaaaa atattcgggt gaatggcatt
    34501 gcgccggggg caatattaac cgatgccctg aaatccgt
//
LOCUS       DAJTNM010000027        33551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTNM010000027 DAJTNM010000000
VERSION     DAJTNM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.027"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            535..672
                     /locus_tag="OO172_001446"
     CDS             535..672
                     /locus_tag="OO172_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511679.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1272..2051
                     /gene="ipaJ"
                     /locus_tag="OO172_001447"
     CDS             1272..2051
                     /gene="ipaJ"
                     /locus_tag="OO172_001447"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5511680.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2999..3928)
                     /gene="virB"
                     /locus_tag="OO172_001448"
     CDS             complement(2999..3928)
                     /gene="virB"
                     /locus_tag="OO172_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5511681.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4145..4381)
                     /locus_tag="OO172_001449"
     CDS             complement(4145..4381)
                     /locus_tag="OO172_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5511682.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="OO172_001450"
     CDS             complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="OO172_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5511683.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTSPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="OO172_001451"
     CDS             complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="OO172_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5511684.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="OO172_001452"
     CDS             complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="OO172_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5511685.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="OO172_001453"
     CDS             complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="OO172_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5511686.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINKTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="OO172_001454"
     CDS             complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="OO172_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5511687.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="OO172_001455"
     CDS             complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="OO172_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5511688.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="OO172_001456"
     CDS             complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="OO172_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5511689.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="OO172_001457"
     CDS             complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="OO172_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5511690.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13569..15185
                     /locus_tag="OO172_001458"
     CDS             13569..15185
                     /locus_tag="OO172_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5511691.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLPQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15195..15557
                     /gene="ipgE"
                     /locus_tag="OO172_001459"
     CDS             15195..15557
                     /gene="ipgE"
                     /locus_tag="OO172_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5511692.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15557..16015
                     /gene="ipgF"
                     /locus_tag="OO172_001460"
     CDS             15557..16015
                     /gene="ipgF"
                     /locus_tag="OO172_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5511693.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16024..17139
                     /gene="mxiG"
                     /locus_tag="OO172_001461"
     CDS             16024..17139
                     /gene="mxiG"
                     /locus_tag="OO172_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5511694.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17147..17398
                     /gene="mxiH"
                     /locus_tag="OO172_001462"
     CDS             17147..17398
                     /gene="mxiH"
                     /locus_tag="OO172_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5511695.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17411..17704
                     /gene="mxiI"
                     /locus_tag="OO172_001463"
     CDS             17411..17704
                     /gene="mxiI"
                     /locus_tag="OO172_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5511696.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17710..18435
                     /gene="mxiJ"
                     /locus_tag="OO172_001464"
     CDS             17710..18435
                     /gene="mxiJ"
                     /locus_tag="OO172_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5511697.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18432..18959
                     /locus_tag="OO172_001465"
     CDS             18432..18959
                     /locus_tag="OO172_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5511698.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18901..19596
                     /gene="mxiN"
                     /locus_tag="OO172_001466"
     CDS             18901..19596
                     /gene="mxiN"
                     /locus_tag="OO172_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5511699.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKR
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19589..19996
                     /gene="mxiM"
                     /locus_tag="OO172_001467"
     CDS             19589..19996
                     /gene="mxiM"
                     /locus_tag="OO172_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5511700.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19980..20408
                     /gene="mxiM"
                     /locus_tag="OO172_001468"
     CDS             19980..20408
                     /gene="mxiM"
                     /locus_tag="OO172_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5511701.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20538..21170
                     /gene="mxiE"
                     /locus_tag="OO172_001469"
     CDS             20538..21170
                     /gene="mxiE"
                     /locus_tag="OO172_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5511702.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21157..22857
                     /gene="mxiD"
                     /locus_tag="OO172_001470"
     CDS             21157..22857
                     /gene="mxiD"
                     /locus_tag="OO172_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5511703.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSRLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22876..23943
                     /gene="mxiC"
                     /locus_tag="OO172_001471"
     CDS             22876..23943
                     /gene="mxiC"
                     /locus_tag="OO172_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5511704.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23956..26016
                     /gene="mxiA"
                     /locus_tag="OO172_001472"
                     /gene_synonym="virH"
     CDS             23956..26016
                     /gene="mxiA"
                     /locus_tag="OO172_001472"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5511705.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26029..26430
                     /gene="spa15"
                     /locus_tag="OO172_001473"
                     /gene_synonym="spaK"
     CDS             26029..26430
                     /gene="spa15"
                     /locus_tag="OO172_001473"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5511706.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26434..27726
                     /gene="spaL"
                     /locus_tag="OO172_001474"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26434..27726
                     /gene="spaL"
                     /locus_tag="OO172_001474"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5511707.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27819..28175
                     /gene="spa13"
                     /locus_tag="OO172_001475"
                     /gene_synonym="spaM"
     CDS             27819..28175
                     /gene="spa13"
                     /locus_tag="OO172_001475"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5511708.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            28144..29022
                     /gene="spa32"
                     /locus_tag="OO172_001476"
     CDS             28144..29022
                     /gene="spa32"
                     /locus_tag="OO172_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5511709.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIAANYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29016..29897
                     /gene="spa33"
                     /locus_tag="OO172_001477"
                     /gene_synonym="spaO"
     CDS             29016..29897
                     /gene="spa33"
                     /locus_tag="OO172_001477"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5511710.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29902..30552
                     /gene="spa24"
                     /locus_tag="OO172_001478"
                     /gene_synonym="spaP"
     CDS             29902..30552
                     /gene="spa24"
                     /locus_tag="OO172_001478"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5511711.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30567..30827
                     /gene="spaQ"
                     /locus_tag="OO172_001479"
     CDS             30567..30827
                     /gene="spaQ"
                     /locus_tag="OO172_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5511712.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30827..31597
                     /gene="spa29"
                     /locus_tag="OO172_001480"
                     /gene_synonym="spaR"
     CDS             30827..31597
                     /gene="spa29"
                     /locus_tag="OO172_001480"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5511713.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31604..32632
                     /gene="spa40"
                     /locus_tag="OO172_001481"
                     /gene_synonym="spaS"
     CDS             31604..32632
                     /gene="spa40"
                     /locus_tag="OO172_001481"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5511714.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSVFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32645..32887
                     /locus_tag="OO172_001482"
     CDS             32645..32887
                     /locus_tag="OO172_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511715.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10475 a         4884 c         6712 g        11480 t
ORIGIN      
        1 tgacacttgt tgactacctg tgcggcatct ggtatcaagg tggcgtgaag ccggttcagg
       61 ttctcatgcg gaacgttgga acctatatcc ccccgatacc aaaggcgaaa tgtaagtgac
      121 aaaatgcaat gaacacgttc tccgttaact gaggagcatg gtgataaata aaggtaaatg
      181 cagaaggtgt agagtctcgc agatttctga catcatagag cctaacatta gatgcataac
      241 gctacagatt gtagaatctc tacaggtgaa atatacactg gttggcgttt atgtcgcaaa
      301 acctgtcatt caggttcatc tcaccaataa ggatatgagt gaagtggagg ataagtgagt
      361 ctgctgtcag agttttctgg tgtatgtcag taactgcgaa ccatgtctgc ttgatatggg
      421 agcttatgga ggctctctgt acaaggttag ggagctaaca aagcagggat aaaaagtccg
      481 cctgatgcag gcacggttcg ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat
      541 gttcaggcta tctttatctt gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca
      601 atagaagaac tgcaggcagt actggtccca catcgtttgc tcagtcaact ggtgaagttc
      661 cgtagcgcgt aaattaatgc tttgcatgaa acgttactgg agtggatgaa agaatacctg
      721 catatatcat tactgcgttg gattaccagt acaacagatt gaatgaactg gacatgttga
      781 tagacggcat tgaaaagcag cttataagca tagtgcggca gagttaattg gagagcactt
      841 cgccttctga gtattaagta agcatggtgg tacctgcgga tactatctat tcactgtgct
      901 cggtcggcag ccttactgac caaggagttg ggggagttga taacaaagtt gaaaaaacag
      961 ctctcaacca aggtggttat tgtaacaata gcaaataagc ttgtgtgacc tgtatcacaa
     1021 taaccgatta tggacgtaaa tacgacaaaa atatgtcagt atcaggcggt attaattgct
     1081 gatgctgatg gacattaact cttaataaaa gggtggatgc tgaaaggttg ttatggtgac
     1141 ggacgtgatg acgacggaaa agagtatgag gttttggatt taaagttcgt tcggggaata
     1201 aggacccggt cagctaattc tcatagtagt tcgcattgct ggatgcaata ctgtgctgca
     1261 attactcgtc gatgtcggaa caacggaagc cttgcaaacg gggatgtatt catacaggtg
     1321 ttatgttata tggggtttta ttgcaggggg ctataccaag agaatatatg atatctcatc
     1381 agactgatgt gagggtaaat gagaatagag taaacgaaca aggttgtttt ttggcgagga
     1441 agcagatgta tgacaattct tgtggagctg ccagtttact ctgtgctgca aaggaattag
     1501 gggttgataa aataccacaa tacaaagggt ctatgtccga aatgacaagg aagagttctc
     1561 ttgatttgga taatcgttgt gagcgggatt tatacttaat aactagtggt aattataatc
     1621 cgagaataca caaagataat atcgcggatg ccggttattc tatgcctgat aagatagtca
     1681 tggctacgag gcttcttgga ttgaatgctt atgtcgttga agaaagtaac attttttcgc
     1741 aagtaattag ttttatttat cccgatgcaa gggacttatt aattggcatg ggatgtaata
     1801 tagtacatca acgagatgtg ttatcttcta accaaagagt tttggaagct gttgctgtat
     1861 cctttattgg agttcctgtc ggcctccact gggtgttgtg tagacctgat ggaagctata
     1921 tggatccagc tgtaggagaa aattattctt gtttttcaac tatggaatta ggtgcaagaa
     1981 gaagtaattc taactttata ggatatacta aaataggtat ttccatagtt ataactaatg
     2041 aggctttgta aattgtcgtt ttttatctgt gaaggagctt gaatattttg gggcgggggg
     2101 ggcataacaa atgatattac tgagtacaaa tttagctata ttgggcggat tagttgtgtg
     2161 ggaagacgaa atgtttctct tgaaataagt gaactccctc aaggaatgat tgtgtgctta
     2221 aatatttaag tgctggtaag tttgagaagg ctgacatatt ttgcagagca aacagtgatt
     2281 gacttggatg atatttttat tttagtcact cgttgtaatt ggagtatgct ttattttatt
     2341 tgaaaataga tgtattttat acccgcaatt taaactgtaa gccctatgag gggtatggat
     2401 attataagtt actctttgac taagcataac ttttggggtt agaaaaataa atctcttgtt
     2461 aataattaat atgattttaa aatcaaactt gtttataatt ataaaataat gtaagtcggt
     2521 cattttatgg gaagattagg agaataaaac agtaattata tttgagaaat ctgctaatat
     2581 attcttaaag cttttttgat actgtgaacg aagtctgtta atggtggatt ttttgtttat
     2641 caccatgtaa aaaaattgag tagtaaaagc gttgactgct aatcctcgct gtttttattg
     2701 aaacgattaa aaatacgctg aatattttcg gccagtcact catcttcaca taaaattttg
     2761 accatcatgc cgcatccctt agttgcacaa ttcattttca atgatatgaa tattactaat
     2821 ttcaccagat aattttctcg tggtcagcag ggtatgagtt ttcaccgtaa ttttaaggaa
     2881 acacagtgcg aggggattga ataacagata gccgacggaa ttaccccacc ggctgaaagt
     2941 caagttcaac gaagattttt attatctgaa ttgggcagtt tacatcagtg ttcgatgttt
     3001 atgaagacga tagatggcga gaaattatat cccgaatagc ttcatctatt tctctctgaa
     3061 ctgttttgtt tattttagat aatgtaaata ttagagttct gtttgtttta ttctcttttc
     3121 tttttatgta agtccgtttg tctttagaaa taaataacga gtcaactttt agcgaagggt
     3181 tttgctttct gtttttgctt ttctttatga tgtttaaaat ttctttttta tatatgtccg
     3241 ggggcaaatt tgtatcaagg tcttttattt cttcctttaa tattggtagt gtagaactaa
     3301 gagattcatt agccttttca aggcctttat aataattgaa taatatcttg tagtcattaa
     3361 agttaagttc tgacgcgatt ggaaatagag atattatttc ctgtggaacg cttgctgcct
     3421 gaaaggcacg agtgactttc gcgcgagaca gattctcttt tttggctatg tctttatagg
     3481 acatccctga tactttcaga aaattaagac caataccaag ttctcggatg ctatgctctt
     3541 ttgctgtttg tatatcgttt gctagttttc tggcatcaag agtagatata tattcttttg
     3601 aatatagaac ttcaagatct gctcctgcat atattgcaga tgctcttcta cgagtgccat
     3661 ccagaatttc aattctacca tcaatctccc ttcctattac agggaagaat tgttgtagct
     3721 ttatagtttt tatgatatcg gctaatgatt cttctgttag ggattcttga tcgcgtacat
     3781 tcgttttttg gttgacgaag gttaaatctt ggatcctttt atgaggaatc ttggctttga
     3841 taaaggaaac tttattacca gaatggagtg taaactcttt cttttggcct tcctttatac
     3901 ttaacaagtc gttgcacaaa tccaccatat cacaccctgt ttattcatat tgatttatat
     3961 tgacaaaagt taaatgcagt gtgagcaaga ttgtatgtaa ctgttgatga tagtcaacgg
     4021 tagtcttaaa aattactatt ttaatagctg gattgatgca atttatcttt attggataaa
     4081 agaataatat tcttgtggag ctctgatgtg agagctccat agatgaaaaa atgcttatca
     4141 attactatat tgattttgta ctatttttga ctacagaaat aagatctcca atagtaataa
     4201 tgtgttctaa tgcagattca tctatacgta gattgaactc aaaactaatc atatccagca
     4261 tttcaatcag agaaagagaa tcggcagaaa gatcttcaat aaatttagtt tcttcagata
     4321 gtttgctgta cgctatacca tagcaaaagg ccactattga taatattttt tcttttatca
     4381 tattttaatc cttattgata ttctttaata cttttgatag ggaagtggtt acatcttttg
     4441 ctgctttata tatattattg ttttcagctg taatatcatt gttatttttt aaatcagata
     4501 tatcatctgt aagtaattct gcagaggtat cgtcaatttg tgacaatacc ttgctgaggg
     4561 cgctagatac ttcttttgcc ttttcataga tcgcatgatt tttatcaatt gtatctgcgt
     4621 tggtttctgt tacgtagttt attttatttg catctgtaac ttttaatttg ttgtttattc
     4681 tgtgttctgt tgtggatttt gtaatgtcct ttgatttttc ctgtaactct cgttcttgtg
     4741 ttcctgattt agttccaatg agagatataa gattacttag ggagtttgtt actttttttg
     4801 aagcttcaaa tatcgtttcc cgtgttaaat atgtgtctcc atttcttgat aaaaaatcag
     4861 tctgaagtac ttttgaatca gtgtttgagt tgaatttatt tatttgcgaa gctactctat
     4921 gagaaagaga agcgctactt ttagttgtat ctgatgagtt atcattactg cttgtcactc
     4981 cttccgggtg gagaggggag gtggttgaag agttctgtat gttcagaggc ctgtgtcccc
     5041 gagaaagaga gttatttgca gatctttctt gactactcgt agttaacagg tctgaaccta
     5101 ttccatccga attatctgga gtatggttga aaaccttttc taaagctgaa ttatttgcaa
     5161 atgtctggac agtatctgat atagagtgtg gtagaacgga agttactttt tgaactagtg
     5221 ctctttgtgg atttagtaat gagtttccgt tacgggataa tagatcgttt gtctgagaag
     5281 taggagactg tgcatcattt tctgggttct cgtgatagct attgtcatat actctgttgt
     5341 caaaaactct attatcgtaa gttctattat cattatttat atgatagtga attacaggtt
     5401 gggatgtttt ctcattggct ccgggggttg gtccgccatc tggcatatct tcgggaggtg
     5461 tagggaggcc atcttggata ggatgcgaat cttttatatc ttctaaaata tcattaagga
     5521 cttttctcaa cagctcagga aacacggcta ctgtatttaa gtctcttgac agtttaccta
     5581 taccaaatcc tttactatca atagtatttt tacgttgatt gcgcaaacta tcaaaggcaa
     5641 gttttgcctg tgcgtcaatt aaatcatcta gtttgagaga tgaaacagtt tcattactta
     5701 actttaattc agcaagaata ttattcttga taaatgatga tacttcagta ttgaggagtt
     5761 tttgtcccaa tgataaagct tcaccagatg tgtgttgttg tgctaatagt gcaagtctgt
     5821 gctcaatgtt ttgtcgtaat tgatggagtt tcttattctt tgccgaccat ggtccaaagg
     5881 cagaatcttg ggataattta tcttttataa ttagttccga atactgtgcg acaatttttt
     5941 gaagtccctt ttcgttttca aaattaagac cattttgatg tactaaggag tacaaaacat
     6001 ttgaaaattc gagaatctcg cttttggcat acgcattcat tgagttggaa taaatgcttc
     6061 ttaacacatc ttctttccct gaggaagaaa ataaaaaatc aagacatttc tgattaaaag
     6121 acgttgcaaa gttttctttt tcagacactg atgctggtgt ttttagagaa gtttgcgaac
     6181 tatggatatc ggatattttg ctttttaact cgctgtattc tgttgatgat ggtgaagttg
     6241 ccttatataa gaatgttggc gcttgagtat tatttacatt atgcatggcg cacctcagaa
     6301 atggagaaaa agtttatctg tatctgtaca tgagcttatt gtactactca aaacctttac
     6361 taaattatca aaaatactat tggcattact gtatttttga actaaagttt gaagattatt
     6421 tttcattgtt tcatcttcgg cagagaatcc ggcattccat gcctgatatt ttgcattatc
     6481 tagcacaacc tcgccatttc cacctagatt atctaagctt tttaacatat tgtctattgg
     6541 ggtcatgttt atactgacaa catatccccc gtttttttga gataccttgc cgattgttcc
     6601 acctaattct gtaagccatt tatttgcttg ttcctgacta acagtattat ttgctggata
     6661 tagcggttta tctttatatt tttccttgag ttcttccaat gcctttttaa gcgagttgac
     6721 ttgtaatttc acggagtttc cgtcgttacc tccgggagag atccagccgg caagactgga
     6781 aagaacagcg ctaaaatctt gatacatttg agtatatgaa ctaacggcat gttcatatac
     6841 tttcagatac tgttcattaa tatcattgat ggagtttgca attttagccc acagttctct
     6901 atgagatatc atttgatctc catcaagctc ggcttctttc ggggctgaat gtaataattc
     6961 tcttgcgtct ttattaattg gatattcgtt cctggaaaga atatccaata gttgagcgga
     7021 tttatttaca tccatgctaa tctgagatga atgtaatgct atttcttcta gcgaagtttt
     7081 agtcaacgtt ttttgtgaaa gctctttctt taatgcctta tttgttgttc tgatattatg
     7141 aagggtgtcg ttaagcatag taagggaact tacaggatga gaactggtcg ttgtttttat
     7201 atcagaatta actgtttcgg ttgatgaacc gttggtattg tttggactga atgatgaggt
     7261 ggaaatacta ttagtcagag ttgttatatt catggttatt tccttacagg aagagccata
     7321 tattattatg gctcttggat atattaagct cgaatgttac cagcaatctg actggctgcc
     7381 gaattccttg attgggtgat gctgtcaatt atattcaata atttttgtat taattgattt
     7441 gtcgcttggg atgcttcttt agatacttgg gatgcttcct ctgcttgttt actgctggcc
     7501 tgactgatta gttgttcttc ttcttcaaga gcagatgcat aacgccctcc tgatgtggat
     7561 atattaccag caacggctga tgtttccatt gttgcacggc caatgttttg tttttgctgc
     7621 gcagaaaggg tattgagctc gtaagttctt ctctgggtgt caattttatc ctgcaaagaa
     7681 atatcgggag aactaagaga agttttatgt tcagttgaca gggatatatt atctggcgcc
     7741 agtttatttt tacctaaaaa ttttgtgcta gagttagttt gtggtgaggt gatatttgta
     7801 tctatttgtt tatttaaccc taatttagaa cctgcaagct ctttttcaag agattgagca
     7861 gtggcaaggt tctttcttaa ggctcctttt tggtcgctaa tccctgaatg cgttttttta
     7921 gcaccgatac ccgttatacc tacttgtgtg actgctccag taatgcttga tgatagggct
     7981 gccaggcctt gccgaacaat gttctctgca gctgattttg tagcatcgaa cgcaatcaat
     8041 gacaattgag agcccaattt attttctgca gtgcggagag cagaaagtag aacggctaca
     8101 ctaataatta aagaaacagc attagaagaa agactggaaa tatccagagt gttctctgga
     8161 gtaagggtgt gactcatctc tgaaagatct ttcatctttt tgtcagtaag gcgagcaatg
     8221 atttctgaat catgctgaac ctgctctgat aactttaaaa gttgatcatc atttaatgtg
     8281 gttgttaata cgggattttt accgacatta agtggaatat gcgctgcaat ctgctgataa
     8341 ttttgtgatt tttgtgtttc ggaagaactt tgagtttgtt ttgtggatat atctgtatat
     8401 aaaatctggg ttggttttgt gttttgaatt tccataacat tctccttatt tgtatcaagc
     8461 agtagtttgt tgcaaaattg cttttgcaac atcagttcta ttagcctgag agttggacat
     8521 tgaggctaat agatctgcaa ttacttcctg caattggccg aatttttcta ttgcttcact
     8581 gatatatttt gacagttgtt caacttgata tttcgataat gtcaggtctg ctagatttgt
     8641 cgacgcgctg ttctggaaaa cagcagaagc gacacttcct cccgcttgtg tggcagagtt
     8701 aacactttct cctaataaaa ctgcttggtt taaatgggtg gaaataattt gtttggatat
     8761 tacttcatca cccgctgcac caagaaattt atttaatctg gcaacagcat tagtgattaa
     8821 atcgtccagt tgagaagaaa aattcttgag aaactttggt ataaggtctg tgagggtttt
     8881 acctattatt ttgccaatat tctctgcaag ttttgctgct gcctgtttac caacagtggc
     8941 tacgagaacg actgctgcaa ctaggacaag agcgcctgcg attgccccca gaatagagcc
     9001 aatcattttg gcttttttcg agtcgacgcc caagccttcg agcatttttg taaatgcatc
     9061 tgaaaggagt ttgattaagg gttcaatgac tgctttcatg atcggattca gggcttgttc
     9121 catgaaggaa ttgccggtcg ctgcttgtac tatagcatcc gtaaccataa gagctaaacc
     9181 aacagctgcc agtgctagag aggctcctcc agaaaaagct gctgcaacaa cactaacgat
     9241 agttaaaagt gccccaagta ttttcccaac acaacccatt actctgttga gttcttctgc
     9301 tttacgtact tcagcagcat actcatcaga ttttctctcc atttcagttt ttcttgattc
     9361 ttggagagac tggaatagag ccagatcatt ttttaaagat tcttcattat tttttccaac
     9421 tagttgaata aaggttgcca tcaattgagt aacactggca agtccggtta atgatttctg
     9481 ctgggttgat agctgttcag cagatgctgt gtttgaaaat gcagaaaaag agtctatttc
     9541 tttttcgagt tgcattgatt tatctgtaag tttgctatga attgacaggg ttttctgctc
     9601 aatcaatgtc ctgtctttaa ctgctgcgtc ttttttgata gtgagttgta tttcttcccg
     9661 gcttaatttt ttcttttctg gtgactctgg gtcgagttcg gataatcttg tttgaatttg
     9721 gttaatttta ttttctaggt cttttatttt agaatctgcg ttttttagtt tattaatttg
     9781 tttttcatag tctctggtta gtccttcagt ttcagataga agagtgttaa ttttatcgga
     9841 gaattctagg tttttttgct gtcttgcctg ttgctgggac ttccaagcag taattttatt
     9901 tgttaatgca gttaaagatt tttcaccgag tatttgaata aggtttccaa ttaaaagcgt
     9961 tagttgggaa cttgcattta atgactttgg tgctttaagt tcagggatta atatatttga
    10021 agttgagtgt gcattagttt tattaatatt ttggttagca gtaagatcag caattgtaag
    10081 agaaaataat ttattagctg catcatttgc agcttggata gtattgtctc caagctcagt
    10141 ggaagccaat attttggcaa gaggaaaacc agtggttgtg gtgcttacat tatgcataat
    10201 aattactcct tgatatcctg aattgcgtcc aagtatgatt gtgcttttat ttttaatttt
    10261 tcatcattgc tgtgttgaat tacgagttcg aagcactctt tagcttttaa gggggctttc
    10321 aaccgaagct ggcattgtcc agtatggaat actggtgtat agtcattttt tcctaatgca
    10381 aaagcgacag cataaaggtc tgctgcttgt tggaactgtt cttttatctg ataaatagct
    10441 gcgagtccca taatgtagtc tacattgtaa aagtcgtata tacataaaaa tctgaagaaa
    10501 acttcagctt cctctattct tcctttgttg taaaagtcat aagcatatga ataaatgtca
    10561 tccatcatat catcaggaat tgcattaata tctttcagtg tagcgccaga gttaattgca
    10621 tcaattactg cagtagagat gctttcattt tcagtgatat ttaaagacat aaggtctcct
    10681 ttttgtctgc tttactttaa taagtataag atttaatata aaagatttaa tttgtattgc
    10741 tttgacggta tacagccttt agaacctctg cgcttatttc tatttgtaaa acttttgttg
    10801 attcattaaa tattttatcg tagacggaac tggatactag atttgtaaga tccttcattt
    10861 gtgtgttaaa caaatccatg tcactgctat agcgttttaa aatctttgct ctgaggccag
    10921 atgaggcaat ctcgtttagt gctgtatgac tgatagatgt ttgtgctgat ttttcatcaa
    10981 gttttatatc cgggaatttt ttttcaatag ctgcaaatat gttttttttg ttatctgagg
    11041 tcaaaacaac tccattgtgt atggccattt catctatact tctgtttatc attgaagaga
    11101 cataagaggt tcgctcttgg ctcatccaac aaaatagttg attccccgaa ttactatctt
    11161 ttattttgtc tatgacgctg tccctgattt tatttatatt caccgggcat ccaaaattct
    11221 tctgaggaaa gcgttgtttt aagttaaaaa cagataatat tttctttaca agcttattgc
    11281 gacttaacga atcaaatgat gggcgaggaa tcgcgaattc aacctgtgga agtattttgt
    11341 ttagaatttg catgataccc cctatatgtt agttcacttc tgaagtgatg tttgcaatgg
    11401 ttagtttaat acaaaataaa aattcataca aagattcaat gagttcatca ggtgaaagat
    11461 attttttgta gtgtttcttg gtcaaaacac attgatgagt atcagggaga agacagatag
    11521 aagaaccata atctctactc aaagagtagt taaggatgag tatgtgttct ggattaataa
    11581 tattttctgt aaaaagttca tgttcattaa gaagtccggt caaaaagatg tattcatcat
    11641 tatctgttat acaaacaggt atttgttctt cgagcaatat aaaggcttga tttttatcgt
    11701 taaaatcaag ctttgcacct gttatttcat aaagtttatc atatagtttg cgacacattt
    11761 ctatggcctt ctatatatta gaatgagagt tattcaataa aagcttattg atctgtaata
    11821 agtcgtgaat gtaaagtttg gttccaagat ctggcgattt aagagaattg taataatcaa
    11881 taatttttat tgatttttcg ataatttctt cttttttatc tgttgcttcg gtttttaatt
    11941 cgtttaatac acgagtcata ttgctgggtg ctgattttac taagtatgag tggcaagcgt
    12001 tgatgaccac atcaaagtct tctgaagata cattttcagt aggatgtttg taaagtgaaa
    12061 ttgataaagg cttgaatgtt tttggtgtgt ggataaggtt aattttattg aaacttttaa
    12121 gatagctgcg tcgtaattga gtcaatctgg aaagatcaaa atcagattca atatacttca
    12181 ttatttcgtt ataagactga ttgagacttt caatgcgttg cctaacttta tttgcatacg
    12241 cgtatacatc gttaggtgta gcaaccaatt taacgtcagg gagtggaaag tatatttcag
    12301 aatttcctgc tttaagaaca ttaagtgcca taccagcaca gttttcagat ttactgatta
    12361 atttataatt tgccaattgt ctgaaagcat ctgctttctt tttgcatata aattttgaca
    12421 tgtttgtttc atcaaggcca aaaaggttat ggctgatttt accatccttt gttttattat
    12481 cacctgataa tggaatgtat actttatcag cactgacccc ccaataacca taggggtctt
    12541 ttttttgatt agctctggga aaaaatattt ttttgttgtt attttgatct tgttttgaat
    12601 ttaataagtt tctaacctct tggcctgcat caagtctttc ggctgtttgg tctgaaatca
    12661 tatttagttt gtcttgctta taggtatcga cactgtaacc taagcgtttt tcaaaaaggt
    12721 agtttatttc tgtatctttt gtaacatccc cgccgggata ccagctgaga taggtatgag
    12781 ttggttcctg tagtaaattg ttttttatac tgatactagt atgccctaaa tgggttatgc
    12841 ctctatttcc ccagcaacga aaaactaaat gtctttcaag atttttggct gtaccgatgc
    12901 catgaaaacg ctctgcggta ttcaacatta atttgatgtc tctacctgtg aaagagtgtt
    12961 gaattgagtt tttagctgct tcataggcgg caaacgcact ttctgcagat ttttcgctag
    13021 ctaatgtatt gaggaaaagc aataaggatt tttgattctc tagtcgataa ttttttagtt
    13081 gttcatttaa tatctgtgaa tttatctttg cattaatagt gcttaatgtt ctgaaaaaga
    13141 gatcctttag gttgaatttc tgtgctatca atataggtcc atgctctgta tcttcaacgc
    13201 gtataggccc ttttgtattg cttgcgtcaa tgaaattgct aattttgagg ctcatacttt
    13261 attaactctc cattacttgg tgattgtggg gatgtaaaat gtatttggca tataatatat
    13321 tcaattaata aatttagaaa cttgagcctg ttaacataat caaattttct tttgctgtac
    13381 ataatatgta cctcgtgagc atatgtagtg ctcgtttcat catgaaatcc cacaagataa
    13441 agtgcctgat gtatcaggct cggagtgtta tagaaaaaga gagaaccctg ttgaataagt
    13501 atcttttttg ttgtaaaacg aggtacaaaa ctgcaatcaa tgccccattt atgactgagg
    13561 ataattaaat gcacataact aatttgggat tgcatcaggt ttcatttcaa agcggagatt
    13621 cctataaagg cgcagaagaa accggaaagc acaaaggtgt aagcgtgatt tcatatcaaa
    13681 gagttaaaaa tggagaaaga aataagggga tcgaagcgct taatcgatta tatttacaga
    13741 accaaacaag cttaacagga aaaagccttt tgtttgcgcg tgacagagct gaagtttttt
    13801 acgaggcaat aaagttggca ggtggtgata cgtcaaaaat taaagccatg atggaacgat
    13861 tagataccta taaacttggt gaagttaata aaagacatat taatgagctt aataaagtaa
    13921 taagtgaaga gataagagca cagctaggca ttaaaaataa aaaggaattg caaacgaaaa
    13981 taaaacaaat atttactgat tatttaaata ataagaattg gggaccggtc gacaagaata
    14041 ttagtcatca tgggaaaaat tatggttttc aattaacacc tgcctctcat atgaagatag
    14101 ggaataaaaa tatatttgtc aaagagtata atggaaaagg aatttgttgt gcttctacga
    14161 gagagagtga gcatatcgcg aatatgtggc tttcaaaagt ggtggatgac gaggggaagg
    14221 aaattttttc gggtatcaga catggagtta tatcagccta tggattgaaa aaaaactcat
    14281 ctgaaagagc tgttgctgct cgtaataaag ctgaggagtt agtaagcgca gcattatata
    14341 gcagaccaga gttattatca caggctttgt ctggtaaaac agtagattta aagattgttt
    14401 cgacttctct tctgacgccg accagtttaa ccgggggaga ggagagtatg ttaaaagatc
    14461 aggtgaacgc attaaaaggg ttaaactcta aaagggggaa gccaacgaaa ttattaattc
    14521 ggaatagtga tggtcttctg aaagaagtaa gcgttaatct aaaagtggtg acatttaact
    14581 ttggtgtaaa tgaattagcg ctaaaaatgg gcttaggctg gaggaatgtt gacaagctta
    14641 atgatgaatc aatatgttct ttgttggggg ataatttcct caaaaatggt gtgattggag
    14701 gttgggctgc agaagcaata gaaaaaaatc caccatgtaa gaatgatgtg atatatctgg
    14761 ctaaccagat aaaagagatt ataaacaaga agttacaaaa aaatgataat ggagagccgt
    14821 ataaattgcc acaaagaatg actctattgg cttatactat aggtgctgta ccttgctgga
    14881 attgtaagag tgggaaggac agaacaggta tgcaggatgc tgaaattaaa agagaaataa
    14941 taagaaaaca tgaaacaggt cagttttctc aattaaatag taaattatcc tcagaagaga
    15001 aaagattatt ttctactatt ctaatgaata gtggtaatat ggaaatccaa gagatgaata
    15061 ctggtgttcc cggaaataaa gtcatgaaaa aattgccgct atcttcgtta gagctatctt
    15121 attctgaaag aataggggac ccaaaaatat ggaatatggt gaaagggtat tcgtcatttg
    15181 tataagagga atatatggaa gatttagcag atgttatttg ccgagccttg ggtatccctt
    15241 taattgatat cgatgatcaa gcaatcatgc ttgatgatga tgtgctaata tatattgaaa
    15301 aagaaggaga ttcaataaat ttattatgcc ctttttgtgc attgcctgaa aatataaatg
    15361 atcttatata tgcgttgagc ctaaattact cagaaaagat atgcttagct actgatgatg
    15421 aaggcggaaa tctaattgcg cgattagatc ttaccgggat aaatgaattc gaggatgtat
    15481 atgttaatac agaatattat atttcgcgtg tgcgttggct gaaggatgaa tttgcgcgaa
    15541 gaatgaaggg gtattaatgt cccgtttcgt atttattttg ctgtgtttta tcccatattt
    15601 agggagagct gattgttggg ataaggctgg tgaaaggtac aatattccat caagtttgtt
    15661 aaaagcgatt gcggaaaaag aatccggatt taataaatct gcagtcaatg ttaataacaa
    15721 tggaagtaaa gattatggta taatgcaaat aaatgacttt cattctaaaa gacttagaga
    15781 aatgggatat tctgaggaaa tgctaattag tcatccatgt ctttctgtac attatgcagc
    15841 aaagttatta aatgagttta tgatgatgta tggaagaggg tgggaggctg ttggagccta
    15901 taatgcagga acgtctccga aaaagaaaaa agaaagatta aagtacgcag aggatatcta
    15961 tagacgctat ttgcggatag cagcagagag caagcagaat aatcgaagga tataagagga
    16021 tttatgtctg aggcaaagaa ctcaaatctt gcaccattca ggttattagt gaaactcaca
    16081 aacggtgtcg gggatgagtt tccgttatat tatgggaata acttaattgt tctcggacgg
    16141 acgatagaaa cacttgagtt tggaaacgac aatttcccag aaaatattat tcctgtaaca
    16201 gatagtaaat cggatggtat aatttatttg accataagca aagataatat ctgtcaattt
    16261 agtgatgaaa aaggtgagca aattgatatc aattcacaat ttaattcatt tgaatatgac
    16321 gggatttctt ttcaccttaa aaatatgagg gaggataaat cgagaggcca tatactaaac
    16381 ggaatgtata agaaccactc ggtgtttttt ttctttgctg ttattgttgt gttaattata
    16441 attttttccc tctctttaaa gaaagatgaa gtaaaagaaa ttgctgaaat tatagatgat
    16501 aagagatatg gaatagtcaa cactggacag tgcaattata ttcttgccga aacacaaaat
    16561 gatgcggtat gggcgtctgt agccttaaat aaaaccggat ttaccaaatg cagatatatt
    16621 ctggtgtcaa acaaagaaat aaatagaatt caacaatata tcaatcaacg attcccattt
    16681 atcaatttgt atgtgttgaa tttagtgagt gataaagcgg aattactggt gtttttaagt
    16741 aaagaaagaa atagttcaaa agataccgaa cttgataaat tgaaaaatgc tctgattgtt
    16801 gaatttccgt atatcaaaaa tattaaattc aactatctta gcgatcataa tgcaaggggg
    16861 gatgctaaag gtattttcac aaaggtaaat gtccagtata aagagatttg tgaaaataat
    16921 aaagttacat attctgtcag agaagaactg acagatgaaa agcttgagct cattaataga
    16981 ctgatttctg aacataaaaa aatatatgga gatcagtata ttgagttttc tgtacttttg
    17041 atagatgatg attttaaagg taaatcatat cttaacagca aagacagtta tgtaatgttg
    17101 aatgataaac actggttttt tttagataaa aataagtgag gataaaatga gtgttacagt
    17161 accgaatgat gattggacat tgagttcatt atctgaaact tttgatgatg gaactcaaac
    17221 attacaaggt gaactaacat tggcactaga taaattagct agaaatcctt cgaatccaca
    17281 gttgctggct gaataccaaa gtaaattatc tgaatataca ttatatagga acgcgcaatc
    17341 caatacagtg aaagtgatta aggatgttga tgctgcaatt attcaaaact tcagataata
    17401 gggagcattc atgaattaca tttatccagt caatcaggtt gatattatca aagccagtga
    17461 ttttcaatct caagagatat caagtctgga agacgtcgtg tcggctaaat atagtgatat
    17521 taagatggat acagatattc aagtatcaca aataatggag atggtaagca atccagaatc
    17581 attaaaccca gaatctttgg ccaagttaca gacgacgctc tcaaattatt caataggagt
    17641 atcattagct ggcacgttag caagaaaaac agtttcggct gttgaaactt tattaaagtc
    17701 ttaatttata tgattaggta taaaggtttt attttattct tgttgctgat gttgattgga
    17761 tgtgagcaac gtgaagagtt aatttctaat ttatctcaaa gacaggcaaa tgaaataata
    17821 tctgtgctag aacgccataa tattactgct agaaaagttg atggaggtaa acaggggatc
    17881 tcggtacaag tcgaaaaggg gacatttgca tcggcagttg atttgatgcg catgtacgat
    17941 ttgccaaatc cggagagagt tgatatctca caaatgtttc ctacagattc attagtgtct
    18001 tctccaagag ctgaaaaggc ccgtttatat agtgctattg agcaacggct ggaacagtct
    18061 ttagtttcta ttggtggtgt tatttcggca aaaatacatg ttagctatga tcttgaagaa
    18121 aaaaatatat cttcaaaacc gatgcatata tcagtaatcg ctatatatga ctcaccgaaa
    18181 gagtctgaac tattagttag taatattaag cgatttttga aaaacacctt ttctgatgtt
    18241 aagtatgaaa atatatctgt catattaact ccgaaagaag aatatgttta tacaaatgta
    18301 caacctgtta aggaagttaa atcggaattt ttaacaaatg aagtaatata tttatttctc
    18361 gggatggctg tactagttgt cattcttttg gtatgggcat tcaaaacagg gtggttcaag
    18421 agaaacaaaa tatgataaga atggatggaa tttataaaaa atatctttca ataatttttg
    18481 atccagcgtt ctatataaat agaaatcggt tgaatttgcc ttctgaactg ttagaaaatg
    18541 gcgtaatcag aagtgagatt aataatctca taattaataa atatgatcta aattgcgata
    18601 ttgaaccttt aagcggggta accgctatgt ttgttgccaa ctggaattta cttccagctg
    18661 ttgcttattt tatcggtagt caagagtctc ggttaattaa tcatagtgag atggttatta
    18721 gttattatgg gggcaaaatc agtaagcaag gggaggccgc tattcgaagt ggtttttggc
    18781 atcttattgc gtggaaggaa aatatatctg ttggtatata tgaaagaata aacttattat
    18841 ttaatccaat tgctttggag ggaaattata cgcctgtaga aagaaatttg tcccgattaa
    18901 atgaaggtat gcaatatgca aaaaggcact ttaccggtat ccagacatca tgcctatgat
    18961 ggtgttgtta taaagagaat tgaaaaggaa ttatgtaaaa ccataaaaga tagagacact
    19021 gagagtaaga ggaaagcaat ttgtgttata aaggaggcta cgaaaaaagc tgaatcactg
    19081 cgtatagatg ctgtatgtga tggttatcaa attggtatac agactgcttt tgagcatatt
    19141 attgactata tttgtgaatg gaagttaaag caaaatgaaa acagaagaaa tattgaggac
    19201 tatataacta gtttgctttc agaaaattta catgatgaga ggatcattag cactttattg
    19261 gagcagtggc ttagttcgtt gcgtaataca gtaacagaat taaaagtggt tttgcccaag
    19321 tgtaatttag cactgagtaa aaagttagaa cttgatctcc ataaatatcg ctcagatgtc
    19381 aagattattt taaaatattc tgaagggaat aattatatat tctgtagtgg taatcaagta
    19441 gtggagttta gtcctcagga tgtaatatca ggtgttaaaa tagaacttgc ggaaaaatta
    19501 acaaaaaatg ataaaaaata tttcaaagag ttagcacata agaaactgag acaaattgca
    19561 gaagacttgt taaaagagaa tcctgttaat gattaatcaa ataaatgcaa gcaatgctct
    19621 gcagcaaaga ttaaatagtg aagaatttgt taatttaaat gagagattat catcttcgca
    19681 atcatttgat gaggatataa tctatgaaat aatgcaatat ttctcacaga gtgaattaaa
    19741 ttccatagat aatgatgaac tgcacaataa gattgaacaa ttattcaatt cacgttttcc
    19801 ttatcttaca gctgcacaaa aaagttcact tcttaataaa ctcatagatg caaatcaata
    19861 tgtcgatttg catgagggat tttatgcatc attgtccata tataacaata ttgatttcta
    19921 tattaagact acaaccttcg attcattaat tagtgtcttt gaagcaggga gagaggcaga
    19981 tgattcgaca tggtagtaat aagttgaaaa tatttatttt aagtatattg ctattaacac
    20041 tgagtgggtg tgctttaaag tcatcatcta attctgaaaa agaatggcat attgttcctg
    20101 taagtaagga ttatttttct attccaaatg atttattatg gtcgtttaat acaaccaata
    20161 aaagtataaa tgtttactct aaatgtatta gtggtaaggc ggtttatagt tttaatgcag
    20221 gtaaattcat gggcaacttt aatgttaagg aagtagatgg gtgcttcatg gatgcacaaa
    20281 agatagctat agataaacta ttttctatgc tgaaagacgg ggttgtttta aaaggtaata
    20341 agataaatga taccatcctt atagagaagg atggggaagt taaattaaaa ttaattcgag
    20401 ggatataatt gtattgtgag taaatataaa ggtctaaata caagtaatat gttttacatt
    20461 tactctagtg gacatgaacc agttaacgtt gagcttgtaa aagataaaga acgtaacata
    20521 attgagctgg ctccagcatg gaagggtttt tttttgtccg aaatcaaaat ataaaattca
    20581 gtgataatgt gaactatcat tatcgcttta acattaatag ttgtgcaaaa tttcttgctt
    20641 tttgggacta tttcagtggt gctctagtag agcatagcca tgctgaaaaa tgtattcatt
    20701 tctatcatga gaatgatctt cgggatagtt gtaataccga atcgatgctt gataagttaa
    20761 tgttaagatt catcttttca tctgaccaga atgtaagtaa tgcactggct atgatacgaa
    20821 tgactgagtc atatcatttg gttctttatc ttctgagaac tattgagaaa gaaaaggagg
    20881 taagaataaa gagtctgaca gagcattatg gtgtgtcgga ggcttatttt agatctttat
    20941 gtagaaaagc tttaggggca aaagtcaaag aacaactgaa cacttggcgg ttagtaaatg
    21001 gtttattaga tgtatttttg cacaatcaaa caatcacttc tgctgccatg aataatgggt
    21061 atgcatcaac atcccatttt tcaaatgaga taaaaacacg actaggcttt tctgcaagag
    21121 aattatcgaa tattactttt ttagtgaaaa aaataaatga aaaaatttaa tattaaatct
    21181 ttgactctct tgattgtatt gttaccattg atagttaatg ctaataatat tgactctcgt
    21241 ttattagaac aaaatgacat agcgaaatat gttgctcaaa gtgatacggt aggatctttt
    21301 tttgaacgtt tttctgcgtt attaaattat cctattgttg ttagcaaaca agctgcaaaa
    21361 aagagaatat ctggggagtt tgatttatct aatccagaag aaatgcttga aaaactgaca
    21421 ttacttgtgg gactcatatg gtataaagat ggaaatgcat tatacatata tgactctgga
    21481 gaattaataa gcaaagttat tttgctggag aatatatcac ttaattatct tattcagtat
    21541 ctgaaggatg ctaaccttta tgatcacaga tatcctataa gaggaaacat tagtgataaa
    21601 acattctata tatctggtcc tccggcattg gttgaactgg tagctaatac agcaactttg
    21661 cttgataagc aggtttcttc tattgggaca gataaagtta attttggggt tataaaatta
    21721 aaaaatacat ttgttagtga cagaacttat aatatgagag gggaggatat tgtcatccca
    21781 ggggtggcta ctgttgtaga acgtttatta aataatggta aggctctgag taacagacaa
    21841 gcccaaaatg atccgatgcc accctttaat attacacaga aagtatctga ggatagtaat
    21901 gacttttcat ttagttcagt taccaatagt tctatattag aggatgtatc acttattgca
    21961 tatccagaaa ccaattctat attagtcaaa ggtaatgatc agcaaataca gattattcgc
    22021 gatattatca ctcagttaga tgtagccaaa cgacatatag aattatcatt atggataatc
    22081 gatattgata aatctgaatt aaataacctt ggtgttaatt ggcaaggaac ggcttcattt
    22141 ggtgattcgt ttggtgcgtc atttaatatg agctcatcag catcgataag cactttggac
    22201 ggcaataagt ttattgcttc agttatggca ttaaatcaga aaaagaaagc aaatgtagtt
    22261 tcaaggccgg tgattttgac acaagagaat attccggcta tatttgataa taacaggact
    22321 ttttatgtgt cattagttgg tgagcgcaat tcttcgttgg agcatgttac gtatggaaca
    22381 ttgataaatg ttatacctcg cttttcatct cggggacaaa ttgagatgtc tttaacgata
    22441 gaggatggaa caggaaatag tcagtcaaat tacaattata ataatgaaaa tacatctgtc
    22501 cttccagaag ttggaaggac caaaatcagt acaatagcga gagttccaca aggcaaaagt
    22561 ttgttgatag gtggttatac gcatgaaacg aactctaacg aaatagtatc tattcctttt
    22621 ctctcctcta ttcctgtaat cggtaatgtg ttcaagtata aaacaagtaa tatcagtaat
    22681 attgtaaggg tttttctgat tcaaccccgt gagataaagg agtcaagtta ttataatact
    22741 gcagaatata aaagcctcat atctgaaaga gaaatacaaa agactacaca aataatccca
    22801 tctgaaacta ctttgctgga agacgaaaaa tcattggttt catacttaaa ttactaacta
    22861 taaagtaggt gatgtatgct tgatgttaaa aatacaggag tttttagctc tgcattcatt
    22921 gataagttga atgcaatgac aaattcagat gatggagatg agactgctga tgcagagctt
    22981 gattctggct tggctaatag caagtatatt gactcatctg atgagatggc ttccgctctt
    23041 tcgtcattta taaacagaag agaccttgag aaactgaaag gaacaaatag tgatagtcag
    23101 gaacgtattt tagatgggga agaagatgaa attaatcaca agatttttga tttaaagaga
    23161 acgttaaaag ataaccttcc tctagatcgg gattttatag acagactaaa gagatatttt
    23221 aaagatccaa gtgatcaagt cttagcatta agggaacttt tgaatgaaaa agatcttact
    23281 gctgaacaag tcgaattatt aactaaaatt attaatgaga taatatcagg tagtgaaaaa
    23341 agtgttaatg ctggaataaa ttcagctata caggctaaat tatttggcaa caaaatgaaa
    23401 cttgaaccac agcttttgcg tgcatgttat cgtggtttta tcatggggaa catatcaaca
    23461 acagatcagt atatagaatg gcttggtaat tttggtttta atcacagaca tacaattgtg
    23521 aattttgtag agcagtcact gattgtagac atggattctg agaaaccgag ctgtaatgct
    23581 tatgagtttg gttttgtgtt atctaaatta attgcaatta agatgattag aacttcagac
    23641 gtaattttta tgaagaaact ggaatcctca agcttgctaa aagatggcag tttaagtgca
    23701 gagcagctat tgctaacttt attatatatt tttcaatatc caagtgaaag tgagcaaatt
    23761 cttacttctg ttatagaagt atcacaagcc agtcatgagg attctgtagt gtatcaaaca
    23821 tatctatctt ctgttaatga aagtcctcat gatatattta aaagtgaaag tgaaagagaa
    23881 attgcgatca atattctacg agagcttgtc acaagtgcat acaagaaaga gctttctaga
    23941 taacaggaga taaaagtgat ccagtctttt cttaagcaag taagtactaa gcctgaatta
    24001 attatattag tgcttatggt tatgatcatt gctatgttga tcataccatt acctacatac
    24061 cttgttgatt ttttaatagg gcttaatatt gtacttgcta ttctcgtttt tatgggatca
    24121 ttttatattg aaagaatatt gagtttttct acattccctt cggttttgtt gataacgaca
    24181 ttatttcgtc tcgctttatc aataagtact agtcgactaa ttttagttga tgcggatgcg
    24241 ggtaaaatta ttactacatt tggccagttt gtcattggtg atagtctggc tgtaggtttt
    24301 gttatttttt caatagtaac tgttgtgcag ttcattgtta taacaaaagg atctgaaagg
    24361 gttgcggagg tagctgctcg cttttctctt gacggtatgc cggggaaaca aatgagcatt
    24421 gatgcggatt taaaggctgg aattattgat gccgcaggag ctaaagaaag acgcagtatt
    24481 ctggaacgtg agagtcaatt gtatggttca tttgatggag caatgaagtt cattaaaggt
    24541 gatgcaattg ctggcatcat tattattttt gtcaatttaa taggaggtat ttctgttggt
    24601 atgagtcaac atggaatgtc cctatctggt gcattatcta cttataccat actaactatt
    24661 ggtgacggat tggtctctca gattcctgct ctgttaattt ctataagtgc aggatttatc
    24721 gttacaagag ttaatggtga tagcgataat atgggacgta acataatgtc ccagatattt
    24781 gggaatcctt ttgttctcat tgttacatca gctcttgcct tggcgatagg gatgttgcca
    24841 ggttttccgt tttttgtttt tttcctgata gcagttactt tgacggcttt attctattat
    24901 aaaaaggtcg tagaaaaaga aaaaagtctg tctgagtctg attctagtgg ctatactggt
    24961 acatttgata ttgataatag ccatgactca tctttggcaa tgatagaaaa tctggatgct
    25021 attagttcag aaaccgttcc tttaatttta ttatttgccg aaaataagat aaatgcaaat
    25081 gatatggaag gtcttattga aaggataaga agtcagttct ttattgatta tggtgtcagg
    25141 cttccaacta ttttatatag aacaagcaat gagcttaagg tcgatgatat tgttttgtta
    25201 ataaatgagg tgcgtgccga tagttttaat atatattttg acaaagtgtg tattacagat
    25261 gaaaatggag atatagatgc tctaggtatt cctgttgttt caacttcata taatgaacgt
    25321 gttatttctt gggtagacgt ttcatataca gaaaatctaa ctaatattga tgctaaaatt
    25381 aaaagtgctc aagatgaatt ttatcaccag ttgtcacaag ctttattaaa caacataaat
    25441 gagatttttg gtatacaaga aacaaaaaat atgttagatc agtttgaaaa tcggtatcct
    25501 gatctattaa aggaagtctt ccgacatgtg actatacaga gaatttctga ggtattacaa
    25561 agattgcttg gagaaaatat ttctgttcgc aatttaaaac ttattatgga gtctttggcg
    25621 ctttgggctc ccagagaaaa agatgtcata acattagttg aacatgtccg tgcatcactt
    25681 tctaggtata tttgtagtaa aatagctgtt tctggtgaga ttaaagttgt gatgctttcc
    25741 ggatatattg aggatgcaat aagaaaaggg ataaggcaaa cctctggtgg ctctttcttg
    25801 aatatggata tagaggtttc ggatgaggta atggaaactt tagcacatgc tttgagagaa
    25861 ttgagaaatg caaaaaaaaa tttcgttctt ttggtatcag tagatatacg taggtttgtt
    25921 aaaagactta tagataacag atttaagagt atactcgtta tatcgtatgc tgagattgat
    25981 gaagcatata ccattaatgt attaaagact atttagtgag gtttaaatat gagtaacatt
    26041 aatttagttc aattagttag agatagtctt ttcacgattg gttgtcctcc atcaattatt
    26101 acagatttag acagccattc agcgattacc atttctttgg atagtatgcc tgctattaat
    26161 atagctcttg tcaatgagca ggttatgtta tgggctaatt ttgatgcacc aagtgatgta
    26221 aaacttcagt catctgctta taacatatta aatttaatgt taatgaactt tagttatagc
    26281 attaatgagc tggtagagct tcatcgttct gatgagtact tacagttaag agttgttatt
    26341 aaagatgact acgttcacga tgggattgtt tttgcagaga ttctgcatga gttttatcag
    26401 aggatggaaa tcttaaatga ggtcttataa tcaatgagct atacaaaatt gctcactcaa
    26461 ttatcttttc ctaatagaat ctcggggcca atcttggaaa caagtcttag cgatgtttcg
    26521 attggtgaga tttgtaacat tcaggctgga attgaaagta atgaaattgt tgcaagagct
    26581 caggttgtag gatttcatga tgaaaaaaca atattaagct tgattggaaa ttctcgtgga
    26641 ctttcacggc aaacgttgat taagcccact gcccagtttc ttcatacgca agtcggccgt
    26701 ggattattgg gagcagtagt caatccttta ggggaggtta ctgataagtt tgctgttacg
    26761 gataacagtg aaattcttta tcgacctgta gataatgctc ctccgctata tagtgaaagg
    26821 gctgcaattg agaagccttt tttaacaggt attaaggtta ttgattcttt actcacgtgt
    26881 ggtgaaggac agcgaatggg gatttttgcg tcagctggtt gtggcaaaac ttttctcatg
    26941 aatatgctca ttgaacatag tggtgctgat atatatgtta ttgggttaat tggtgagcga
    27001 ggtcgagagg ttacagaaac ggttgattat ttgaaaaact ctgagaaaaa aagcaggtgt
    27061 gttttagtat atgcaacttc ggattactct tcggttgatc gttgtaatgc tgcatatata
    27121 gccactgcta tagccgaatt ttttaggact gaaggacata aagtagcgct ttttattgat
    27181 tcattaacaa ggtatgccag agcattacgt gatgtggcct tagccgctgg agaatcacct
    27241 gccagaagag gctatccggt ttcggttttt gatagcttac ccagacttct tgaaaggcca
    27301 ggaaagttaa aggcaggtgg ctctattact gcattttaca ctgttctttt ggaggatgat
    27361 gattttgctg atccattagc tgaagaggta agatccattt tagatggaca tatatatttg
    27421 agcagaaatc tagcccaaaa aggacaattt cctgcaattg attccttaaa aagtataagc
    27481 agggtattta cacaggttgt tgatgaaaaa catcgtatta tggccgctgc atttcgggag
    27541 ctactttctg aaatagaaga gctaaggaca attattgact ttggtgaata caaaccgggg
    27601 gagaatgcct ctcaggataa aatctacaac aaaatatctg ttgttgagag ttttctgaag
    27661 caagattatc gactcggttt tacttatgag cagacaatgg agcttattgg tgaaacaatt
    27721 agataaggtt ttaaaaatca aagataaata tcaacggtct gttaaattaa ttgaagcaca
    27781 catattaaca ctaaaaaaaa aactctttgt atcgagatgt ggaggcatta gataaaagga
    27841 ttatatactt cctgcaactg gagaatgact tagaacctgt aggcgctcag agcgttagtc
    27901 agcttttcaa tacaagacgt aagatcgcta tagtaaaaaa gcatattatc caatatcagt
    27961 ctgaacgaat tcttctaaag ggaagaattg aagaaattca aaaagatatt gatgaggcaa
    28021 atgcttctaa aagaaaactg cttcataaag agagtaaaat atgtaaacgg attggcttga
    28081 ttaaaagaaa taactttgcc aaacagttga tacttgatga gttatcacaa gaggatatga
    28141 agtatggcat tagacaatat aaacctaaat tttagtagtg acaaacagat agaaaaatgt
    28201 gagaaactat cttcaataga taatattgat agtctcgttt tgaagaagaa gaggaaggtt
    28261 gaaattcctg agtactcttt aattgcagct aactatttta ctattgataa gcactttgaa
    28321 cataagcatg ataaaggaga aatttatagt ggcattaaaa atgcgttcga acttagaaac
    28381 gaacgagcga catattctga tattccagaa tcaatggcca ttaaagaaaa tattttgata
    28441 ccagatcaag atatcaaagc aagggaaaaa ataaatatcg gcgatatgag ggggatcttt
    28501 tcatataata agagtggaaa tgcagacaag aacttcgaaa gaagtcatac ttcttctgta
    28561 aaccctgata atctgctaga atctgataat agaaatggtc aaattggttt aaaaaatcat
    28621 agcttgtcta ttgataagaa tattgctgac atcatttctt tactaaatgg aagtgttgct
    28681 aaatcatttg agctccctgt aatgaataaa aatactgcag acataacccc atccatgtca
    28741 ttgcaagaaa aatcaatagt tgaaaatgat aaaaatgttt ttcaaaaaaa tagtgaaatg
    28801 acttaccact ttaaacagtg gggggctgga cattctgtta gtatttcagt ggagtctggt
    28861 tcttttgttc taaaaccgtc agatcaattt gtaggaaata aacttgactt aattttgaaa
    28921 caagatgctg agggtaatta cagatttgat agcagtcaac ataataaggg gaataaaaat
    28981 aatagtacag gatataatga acagagtgaa gaagaatgct aagaattaaa cattttgacg
    29041 ctaacgaaaa actacagatt ttatatgcaa agcaactctg cgagcgtttt tcgattcaga
    29101 cattcaaaaa taaatttaca ggcagtgaaa gtttagtcac tcttacttcc gtgtgtgggg
    29161 attgggtaat tcgtattgat acattatctt ttttgaaaaa aaaatacgag gtattttcag
    29221 gattttctac acaagaatct ttactgcatt tatcaaaatg tgtctttata gagtcgtcat
    29281 ctgtattttc gattccagaa ctgtctgata agattacttt ccggatcacg aatgaaatcc
    29341 agtatgcaac tactgggagt catttatgct gtttttcctc ttctttaggt attatttatt
    29401 ttgacaagat gccggtatta cgtaatcaag tttctcttga cttattgcat catcttttag
    29461 agttttgctt aggttcatct aatgtaaggc tggctacttt aaaaagaatt cgcactggtg
    29521 atataatcat agttcagaaa ctttataatt tattattgtg taatcaagtt attattgggg
    29581 attatattgt gaatgataat aatgaggcaa aaattaatct gtcagaaagt aatggtgagt
    29641 cagaacacac agaagtttct ttggcattat tcaattatga tgatatcaat gtaaaagtgg
    29701 actttattct tttagaaaaa aatatgacaa tcaatgaact aaaaatgtat gtagaaaacg
    29761 aattattcaa gtttcccgat gacatagtta aacatgtaaa tattaaagta aatggttctt
    29821 tggttgggca tggggaactt gtttctattg aggatggtta tggtatcgag attagttctt
    29881 ggatggtaaa ggagtaatct catgctgagt gacatgtccc tcatagctac actttctttt
    29941 tttacgctat taccattttt agttgctgct ggtacctgtt atataaagtt ttctattgtt
    30001 tttgtaatgg tacgaaatgc acttggtttg cagcaggttc catcaaatat gactctcaat
    30061 ggtattgcat tgattatggc tttgttcgtg atgaaaccca ttattgaagc tggttatgaa
    30121 aattatctaa atggccctca aaaatttgat acaatatctg atattgtccg tttttctgat
    30181 tctggcttga tggaatataa gcaatacttg aaaaagcata cagatcttga gttagcacgt
    30241 tttttccaaa ggtctgaaga agaaaatgct gatttaaaga gtgcagagaa taatgattat
    30301 tcattgtttt ccctcttgcc tgcatatgcg ctaagtgaaa tcaaagatgc gttcaaaata
    30361 ggtttttatt tatatttgcc atttgttgtt gttgatcttg ttatttcaag cattttactt
    30421 gcacttggta tgatgatgat gagtccaatc acaatatcag taccaataaa acttgtatta
    30481 tttgttgcgc ttgatgggtg gggtatcctg tctaaggcct tgattgaaca atatatcaat
    30541 attcctgctt agatgatgga ggtaatgtgt ctgatatagt ttatatgggt aataaggctc
    30601 tttatttaat ccttatcttt tccttatggc cagtaggtat agctacggtt attggattat
    30661 ctattggttt gttacagaca gtgactcaac tccaagagca gacacttcct tttggtataa
    30721 agcttatagg tgtctcaata tctttgctac ttctttctgg atggtatggt gaggttttat
    30781 tgtctttttg tcatgaaata atgtttttaa ttaagagtgg ggtttgatgg acatttcaag
    30841 ctggttcgaa agtattcatg tgtttttaat actcctgaac ggcgtttttt ttagattggc
    30901 tccattattt ttctttcttc catttttaaa taacggtata atttctccat ctattagaat
    30961 acctgtgatt tttcttgttg catcaggatt aattacttct ggtaaggtag acataggttc
    31021 ttctgttttt gaacatgttt atttccttat gttcaaggaa ataattgttg gcctccttct
    31081 ctctttttgc ttgtctcttc ccttttggat atttcatgct gttggtagca ttattgacaa
    31141 ccagcgtggg gcaacgctta gtagttcaat tgatcctgcc aatggtgttg atacgtctga
    31201 gttggcaaaa tttttcaatc ttttttctgc agttgtattt ctatacagtg gtggtatggt
    31261 ctttatttta gaatccatac aattgtctta taatatatgc ccgttatttt ctcaatgttc
    31321 tttccgtgtc tcaaatatct taacatttct gactttattg gcaagtcagg ctgttatttt
    31381 agccagtcct gttatgatag tattgttact atcagaagta ttacttggtg tattatcgag
    31441 atttgctccg cagatgaatg ctttttccgt atcattaact attaaaagtt tacttgcaat
    31501 atttattatt ttcatctgtt cttctactat ttacttttct aaagttcaat ttttcctcgg
    31561 tgaacataaa tttttcacaa atctatttgt tagataaaat attatggcaa ataaaacaga
    31621 aaagccgaca cctaaaaaac taaaggatgc cgcaaaaaaa ggacagtcat ttaaatttaa
    31681 ggatttaacg actgttgtta ttattctggt agggacattt actataatat cattcttttc
    31741 cttaagtgat gtaatgctct tatacagata tgtaataatt aatgacttcg aaattaatga
    31801 gggtaaatac ttttttgcag tggttattgt cttttttaag ataattggct tcccactttt
    31861 tttctgtgtt ctttcggctg tgttgccaac attggttcaa acaaagtttg ttcttgcgac
    31921 taaagctatc aagattgatt tttcagtatt aaaccctgtt aaagggttaa aaaaaatatt
    31981 tagtataaag acaataaaag aatttttcaa aagcattctg cttcttatta ttctagcatt
    32041 aacaacctat ttcttttgga ttaatgaccg aaaaataatt ttttctcagg tgttttctag
    32101 tgttgatggc ttatatctta tttgggggag gctgtttaag gatataatat tatttttctt
    32161 ggcattttct gtttttgtta ttattcttga ctttgtgatt gagttcattt tatacatgaa
    32221 agatatgatg atggataaac aggagataaa aagagaatat atagagcaag agggacactt
    32281 tgagacaaag tcgagaaggc gtgagttgca tatcgagatt ctttcagagc agactaaatc
    32341 tgatatacgt aattcaaaat tagtggtaat gaacccgact catattgcaa ttggtattta
    32401 ttttaatcca gaaatagcgc ctgcaccttt tatttctctc attgaaacta accagtgtgc
    32461 cttggctgtc agaaaatatg caaatgaagt tggtataccg actgtgcgtg atgtgaaatt
    32521 agctagaaaa ctatataaaa cacatacaaa atatagtttt gttgattttg aacacttgga
    32581 tgaagtccta cgtcttattg tttggcttga gcaggttgaa aacactcatt aataagtaaa
    32641 ggagatgtgt tatatgggag ttaatttctg taataaaata ggtattgatc agtctgaatt
    32701 tgaaatagaa tcttccatca ttaactccat tgctaatgag gtattgaacc caatatcttt
    32761 ccttagcaat aaggatataa taaatgtttt actcagaaag atttcatctg aatgtgacct
    32821 ggttagaaaa gacatttatc gttgtgctct ggagttggtc gttgaaaaaa cacctgatga
    32881 tttataatca ttcataataa ctcttttgct gatataaata tttttgttga agatatttat
    32941 gtagtcacag gatcaattgt agaaaattgt tatgtttttg ttaggaactg cactaaataa
    33001 tatatgataa gacagcaaaa acgactgacc ataatccttc ttttattagg agttgataag
    33061 agagactatt catcctgtaa tgttaaaact ctgttatata gtattcgcga ttatgcaaag
    33121 tctgtaaatg atcatgagat actaaccgag agtaatcgtt tattatctca ttgcatttca
    33181 gatagtaacg gagctttctt taaaagtagt aaatacgttc cccttaagta cttacgtaaa
    33241 aggagaatag cacgaaagat accaaatgat tagcactttg ctagtaagct tgctggtaaa
    33301 ctatattgac cgttgggttt gttttgagac tgtaatttaa attgtgtaat tgcctgtttt
    33361 tgatatgttt actccaacaa cggagacagg taaattatgg acgaaaagaa gcttaaagcc
    33421 cttgcggctg aactggctaa aggcctcaaa actgaggccg acctcaatca gttttcccgt
    33481 atgctgacga aatatcccgg ctcttaaaac gatagtggct caggctttca tttttcagcg
    33541 ttccccagaa g
//
LOCUS       DAJTNM010000028        33128 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTNM010000028 DAJTNM010000000
VERSION     DAJTNM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.028"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..223
                     /locus_tag="OO172_001483"
     CDS             <1..223
                     /locus_tag="OO172_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCR5511716.1"
                     /translation="PQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVL
                     DGKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            397..1166
                     /locus_tag="OO172_001484"
                     /pseudo
     CDS             397..1166
                     /locus_tag="OO172_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1199..2719)
                     /locus_tag="OO172_001485"
     CDS             complement(1199..2719)
                     /locus_tag="OO172_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5511717.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNERAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2810..3529
                     /gene="tonB"
                     /locus_tag="OO172_001486"
     CDS             2810..3529
                     /gene="tonB"
                     /locus_tag="OO172_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5511718.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(3569..3967)
                     /gene="yciA"
                     /locus_tag="OO172_001487"
     CDS             complement(3569..3967)
                     /gene="yciA"
                     /locus_tag="OO172_001487"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5511719.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4072..4611)
                     /locus_tag="OO172_001488"
     CDS             complement(4072..4611)
                     /locus_tag="OO172_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5511720.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4641..5384)
                     /locus_tag="OO172_001489"
     CDS             complement(4641..5384)
                     /locus_tag="OO172_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5511721.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(5412..5501)
                     /locus_tag="OO172_001490"
                     /pseudo
     CDS             complement(5412..5501)
                     /locus_tag="OO172_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5741..6379
                     /gene="ompW"
                     /locus_tag="OO172_001491"
     CDS             5741..6379
                     /gene="ompW"
                     /locus_tag="OO172_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5511722.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(6439..6945)
                     /locus_tag="OO172_001492"
     CDS             complement(6439..6945)
                     /locus_tag="OO172_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5511723.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDCVEAKK"
     gene            complement(6991..7491)
                     /locus_tag="OO172_001493"
     CDS             complement(6991..7491)
                     /locus_tag="OO172_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5511724.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(7577..7756)
                     /locus_tag="OO172_001494"
     CDS             complement(7577..7756)
                     /locus_tag="OO172_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5511725.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(8137..8943)
                     /gene="trpA"
                     /locus_tag="OO172_001495"
     CDS             complement(8137..8943)
                     /gene="trpA"
                     /locus_tag="OO172_001495"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5511726.1"
                     /translation="MERYESLFAQLKERKEGAFIPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(8943..10136)
                     /gene="trpB"
                     /locus_tag="OO172_001496"
     CDS             complement(8943..10136)
                     /gene="trpB"
                     /locus_tag="OO172_001496"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5511727.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(10148..11509)
                     /gene="trpCF"
                     /locus_tag="OO172_001497"
     CDS             complement(10148..11509)
                     /gene="trpCF"
                     /locus_tag="OO172_001497"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5511728.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(11510..13105)
                     /gene="trpD"
                     /locus_tag="OO172_001498"
     CDS             complement(11510..13105)
                     /gene="trpD"
                     /locus_tag="OO172_001498"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5511729.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(13105..14667)
                     /gene="trpE"
                     /locus_tag="OO172_001499"
     CDS             complement(13105..14667)
                     /gene="trpE"
                     /locus_tag="OO172_001499"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5511730.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDNALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(14689..14781)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            14940..15821
                     /gene="yciV"
                     /locus_tag="OO172_001500"
     CDS             14940..15821
                     /gene="yciV"
                     /locus_tag="OO172_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5511731.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            15818..16438
                     /locus_tag="OO172_001501"
     CDS             15818..16438
                     /locus_tag="OO172_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5511732.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            16539..17411
                     /gene="rluB"
                     /locus_tag="OO172_001502"
     CDS             16539..17411
                     /gene="rluB"
                     /locus_tag="OO172_001502"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5511733.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(17451..18041)
                     /gene="cobO"
                     /locus_tag="OO172_001503"
     CDS             complement(17451..18041)
                     /gene="cobO"
                     /locus_tag="OO172_001503"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5511734.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(18038..18796)
                     /locus_tag="OO172_001504"
     CDS             complement(18038..18796)
                     /locus_tag="OO172_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5511735.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            19016..20065
                     /gene="sohB"
                     /locus_tag="OO172_001505"
     CDS             19016..20065
                     /gene="sohB"
                     /locus_tag="OO172_001505"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5511736.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKETKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(20101..20352)
                     /locus_tag="OO172_001506"
     CDS             complement(20101..20352)
                     /locus_tag="OO172_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5511737.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            20732..23329
                     /gene="topA"
                     /locus_tag="OO172_001507"
     CDS             20732..23329
                     /gene="topA"
                     /locus_tag="OO172_001507"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5511738.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPSKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            23539..24513
                     /gene="cysB"
                     /locus_tag="OO172_001508"
     CDS             23539..24513
                     /gene="cysB"
                     /locus_tag="OO172_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5511739.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            24808..24972
                     /locus_tag="OO172_001509"
     CDS             24808..24972
                     /locus_tag="OO172_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5511740.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLTVFLGSALFWVV
                     VALLIWKVWG"
     gene            24975..25142
                     /locus_tag="OO172_001510"
     CDS             24975..25142
                     /locus_tag="OO172_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511741.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            25256..25351
                     /locus_tag="OO172_001511"
     CDS             25256..25351
                     /locus_tag="OO172_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511742.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            25515..28190
                     /gene="acnA"
                     /locus_tag="OO172_001512"
     CDS             25515..28190
                     /gene="acnA"
                     /locus_tag="OO172_001512"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5511743.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(28254..28844)
                     /gene="ribA"
                     /locus_tag="OO172_001513"
     CDS             complement(28254..28844)
                     /gene="ribA"
                     /locus_tag="OO172_001513"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5511744.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYADISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            29014..29778
                     /gene="pgpB"
                     /locus_tag="OO172_001514"
     CDS             29014..29778
                     /gene="pgpB"
                     /locus_tag="OO172_001514"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5511745.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWTLVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            29927..30235
                     /gene="lapA"
                     /locus_tag="OO172_001515"
     CDS             29927..30235
                     /gene="lapA"
                     /locus_tag="OO172_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5511746.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            30242..31411
                     /gene="lapB"
                     /locus_tag="OO172_001516"
     CDS             30242..31411
                     /gene="lapB"
                     /locus_tag="OO172_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5511747.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            31604..32341
                     /gene="pyrF"
                     /locus_tag="OO172_001517"
     CDS             31604..32341
                     /gene="pyrF"
                     /locus_tag="OO172_001517"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5511748.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNAHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            32341..32667
                     /gene="yciH"
                     /locus_tag="OO172_001518"
     CDS             32341..32667
                     /gene="yciH"
                     /locus_tag="OO172_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5511749.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(32793..33011)
                     /gene="osmB"
                     /locus_tag="OO172_001519"
     CDS             complement(32793..33011)
                     /gene="osmB"
                     /locus_tag="OO172_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5511750.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVISHQVGK"
BASE COUNT         8400 a         8004 c         8420 g         8304 t
ORIGIN      
        1 tccacagttt tcacctgaag cattaaataa tctgaaaggt cgagtggaaa cattcacaca
       61 agatttaaag gttatcaaac cgtcagtaaa tattcagcct cagacaatca atatcacaac
      121 tcagacggta cttgatggaa aggttattga tgaaagaacc acatctcata ttaatcgtat
      181 gcaggaagat accttgatta gctcagctta cccagaggaa taaacaatgt tagaactact
      241 aatatttatt gccacacatg gcaatctatt aatctttatc tagtatgacg attcaactat
      301 gaatagtcga atgctacgtg atttcttctg agcagatacc aacatcaaaa attagatttc
      361 acgtagctca cgcttaacag aataggaata acaacaatgt ttgacacaaa gcaaattaga
      421 tactcaggta aagatggtat ctattttcac cttagagata atgtagaatc ttttcttgcc
      481 ttaacagcta ctgaacagat ggactatgac tcaccgatga tggtgacaac caggctatgc
      541 aatcggggca aactatcact gataatgttc agagagcacc taagaccatc tctattagcg
      601 gtgttgtagt ggttgattcg acgggttcag ttctattaac cagacagggg caacttgtag
      661 agacctttat ttctacggtt gaacaatggc gagaccagaa gcaaatcatc actattgttt
      721 gtaaagatgg aatatctatc actgatagta tcattactca gtttaaagcg agtaaagagc
      781 ataccattgc taatggtctt cgtattcaga tgaccttcca aggtgtagaa tttcgttcaa
      841 tcgtagggca aactgatatt tcagcagcta ctggcaagac cgctaccacg aatgatggtg
      901 gtgctaccag taaaaagaac actggcaaca ctacaacgag tcttggaagc cctatgctga
      961 cctgtaaaga gctttttagc tattcggcaa gtgagttatc agatgaagcg ctaaaagccc
     1021 gtgtaacgtg ctctaagagc gttagcgtta aaaatggtga gagcaccttc acagcctcag
     1081 ctaatgagcg agcttcaaaa gtgcttaagt cgggtaatgc tttacggaag tactctgtga
     1141 atccgaataa gaagggcact tattgagaca taaaaagggg cttcatagcc cctgaaaatt
     1201 acagttgata agcatcaata tccagtagct ctttatcagc aatttcaaag attggtgacc
     1261 catcatttag cggtagcggg tctgtaggta tggtgatttc agtgtcgttc tgtagcgtta
     1321 tagttactga acccttaccc aatgtaagcc cttttagatg tttggacaaa attaattgac
     1381 actcgacacg acccgcttta gttgtcaggt caatatcttt aagctggggt agttccttat
     1441 cagtgagtga agccttacgc ttcatagtat caatcgtggt ctgtagttct gtgcgcttct
     1501 ggttaagttc cttaagctga atttttagtt catcaatatc atccatctca agaagtaggg
     1561 cggtagcttt cttgattctg gctgtgacaa tttcaacctc atgctgtaac gctgcaatct
     1621 tcctctctga ttctccatca ttccccacgt caatcatccc cagaaaatta tcaatcatcc
     1681 atcggtcaaa cggtttacgc ttcaacggtg gaacatcaca gcgaccgtct aagtggttat
     1741 tgcacctgta gaccccagca tacttattct ttgttccggt aggatggaca ctagccccac
     1801 actgacacct aaacagacct ttgaagatgt taatcgttag tgggttgtca cttgcaggtg
     1861 tacggccttt acgattctta tcaagaatct cttgagcttt attgaacgtt gcagcatcta
     1921 tagcggcttc gtagtagttc ggtatagcgg ggcggtcatt gcgctgtgat gctggtagtg
     1981 tacctatcgt tgtctctgac cttaagatag tacgcacaga atgaacgcta aatccattag
     2041 catctttacg tcgtgagaat gaactaatgt tattagcctg taggtgacgt gcaatgttat
     2101 gcagactttc accatctagc agcagcttga aaatcaagcg tacagtttca gcacgttcat
     2161 tgagtttaaa agtctcaggg tcaatccatc cgggaacatt ccttgttaaa cgttgttcac
     2221 cagcaagggc aagctttgca cgttgttccc atttctgact aacacggaaa gacttctgtt
     2281 tactctcctg atgcgctatg tagcttttaa cccgctgaat gattgagatt cctaaatcat
     2341 cggatagagc atcccttgtg tagattagcc catcctgtag agtgtgaatc tcgataccat
     2401 gagcaataat gcgattcatc aaggcttctg tttcaagatg ttcaaggcgg cctatgcggt
     2461 cgatagcttc gactagtaaa attgtccctt ctggaaactc ccctgattca atgcgggaga
     2521 gcatatcccc aaattgccct gataagtgtt tacctttgta accggataga ccttcatcat
     2581 tgaaagatgc cactacctga gcatcgctat tcttccttaa ccactgtttg aatagctcat
     2641 tttgtcgtct ggttgaatca gcaccttcag caccttgtgt ggctgatgag aatctcaagt
     2701 atgttattac ggttctcacg gtgataaact cccatgcaat tgattatcat ttaaagatga
     2761 acttataaac gttatggcta atgctacagt gccgaatagc tacaatacaa tgccccttga
     2821 tttacctcgc cgcttcccct ggccgacgtt actttcggtc tgcattcatg gtgctgttgt
     2881 ggcgggtctg ctctatacct cggtacatca ggttattgaa ctacctgcgc ctgcgcaacc
     2941 aatttctgtc acgatggttg cgcctgctga tctcgaaccg ccacaagccg ttcagccgcc
     3001 accggagccg gtggtagagc cagaaccgga acctgagccg atccccgaac cgccaaaaga
     3061 agcaccggtg gtcattgaaa agccgaagcc gaaacctaag ccaaaaccga agccggtgaa
     3121 aaaggtacag gagcagccaa aacgcgatgt caaacccgta gagtcgcgtc cggcatcacc
     3181 gtttgaaaat acggcacccg cgcgccccac atcaagtacg gcaacggctg caaccagcaa
     3241 gccggttacc agtgtggctt caggaccacg cgcattaagc cgtaatcagc cgcagtatcc
     3301 ggcacgagca caggcattgc gcattgaagg gcaggttaaa gttaaatttg acgtcacgcc
     3361 ggatggtcgc gtggataacg tacaaatcct ctcagccaag cctgcgaaca tgtttgagcg
     3421 tgaggtgaaa aatgcgatgc gcagatggcg ttatgagccg ggtaagccag gcagtgggat
     3481 tgtggtgaat atcctgttta aaattaacgg caccaccgaa attcagtaag cagaaagtca
     3541 aaagcctccg accggaggct tttgactatt actcaacagg taaggcgcga ggttttcctt
     3601 caggatcaac cgcgacatac ttaaataatg cttctgtcgc tttatagcgt tgcccgattg
     3661 gttcagacgc tacttttttc acccacactt caatattaat gctgaccgat gtcgtccctt
     3721 tctggacaca gcgtgcatag cagcacacca catcgccgac cgcaaccggc cgtaagaaag
     3781 tcattccttc aacccgcaca gtcactacgc gaccgtgggc aatttctttt gccagaatag
     3841 cgccgccaat atccatttgt gacattaacc aaccaccaaa gatgtcacca ttggcattgg
     3901 tatcggcggg catggctaaa gtacgtaaaa caagatcgcc ctgagggacg ttatgtgttg
     3961 tagacatggt aaaaccgact taaaaggaaa atcacaggcg cgatgctact atgattttcc
     4021 tgaggagaac agaggagata cagcgccagc cccgaaggac tggcagtctg gttaggattt
     4081 atcttcctgc ggcatgtggc ggtagatata gataccgctt aacaatgtga agattaaggt
     4141 aagggcggtc aggccaaaga ctttaaagtt gacccaaata ttttgcggca gccagaatgc
     4201 gatgtagatg tttgccagac cgcaaaggat aaagaaaaca gcccaggcca gatttagctt
     4261 cgaccatacc ggttgcggca gcgtgagttc tttacccagc atccgctgaa ttagcggctt
     4321 tttcatcacc cattggctga ctaacagggc taccgcaaac agagcataaa tgactgtaac
     4381 cttccattta ataaactcat cattgtggaa gaacagcgtc aagccaccga agacgaccac
     4441 cagaacaaaa gtgatcaggg ccatcttctc aaccttacga aagcgaaccc agctatagat
     4501 aagcacaatc gccgtggcga cgatcagcgc tgcagtagcc gcatagatgt cataaatctt
     4561 gtaaaacgcg aaaaagacaa ccagcggtaa aaaatcaaga aactgcttca ttttacgatt
     4621 ccgtaatcaa atgcttaagg ttattggcga atcaacatat acaggcggaa cagatagatg
     4681 agcaatatgg cagaaatcag gttgctcaag gtgttcgcca gtacggcacc aatttccggg
     4741 gttaatgcgg caaaagaaga ggcaaaaagc agcaacagtg tttttgccag caaccagctc
     4801 agtactgcgg gtgccaccag acgcatattc gcccaagtca gccgcatact gctacgcatc
     4861 gaggcaaaaa tgcccatttt gtcctgtacc agcatcaccg gtgccagggc cagtaaaatg
     4921 gccatgataa ttcccggaac gaccaccagc atgatgccaa tctgtactaa aagggtagtc
     4981 agaaaaatca gaataaataa cttgggcaat atcggcgcac tggcaccaat agctcgcagc
     5041 gcacagactc tctgacccgc agacaccagc tggataatta atattacgcc tccggcgaga
     5101 atggcgttac cgattaattc tgaaaaagtg gacgccgctg aagcctgcag caaaatttgt
     5161 tgctgttccg gtgacatatt ctgaaccagg tcgaacaacc cactactgcc gctaacgggc
     5221 acgccgtcat tgagctgcgc aagctgtgca tcactgggtg agaaaacatg ccctaacacc
     5281 actgtgataa acgcacatag caacgatacc aacagaatgg tcataaattg attacggaag
     5341 aaatttccgg tgtcacggta tacggactgc gccgtgatag acatgcactc tccttgagtt
     5401 ttatgcaggt gttaattagc gggcaattgt accctggtta agcctcgggt ggcagcatca
     5461 ggtcttgtat ggaaaagcat atctttgtaa agcggaggta attgctggcc ttcaggcgtg
     5521 ggcaaaccgc ggcaagtggc gatcgagtta atatttgcgt agagaaaata tttaaaaatt
     5581 gatttaaatc acattaacca ggattctcaa tgcaacttct aaattaatcc agatcaataa
     5641 atggtgaatt atcatatgta atgtgatcta tgtaggatca tttgttactc caatgtaggt
     5701 atattcgtca cgtttttata accataacga cggagcggat atgaaaaagt taacagtggc
     5761 ggctttggca gtaacaactc ttctttctgg cagtgccttt gcgcatgaag caggcgaatt
     5821 ttttatgcgt gcaggttctg caaccgtacg tccaacagaa ggtgctggta gtacgttagg
     5881 aagtctgggt ggattcagcg tgaccaataa cactcaactg ggcttgacgt ttacttatat
     5941 ggcgaccgac aacattggtg tggaattact ggcagcgacg ccgttccgcc ataaaatcgg
     6001 cacccgggcg accggcgata ttgcaaccgt tcaccatctg ccgccaacac tgatggcgca
     6061 gtggtatttt ggtgatgcca gcagcaaatt ccgtccttac gttggggccg gtattaacta
     6121 caccaccttc tttgataatg gatttaacga tcatggcaaa gaggcggggc tttccgatct
     6181 cagtctgaaa gattcctggg gagctgccgg gcaggtgggg gttgattatc tgattaaccg
     6241 tgactggttg gttaacatgt cagtgtggta catggatatc gataccaccg ccaattataa
     6301 gctaggcggt gcacagcaac acgatagcgt acgcctcgat ccgtgggtgt ttatgttctc
     6361 agcaggatat cgtttttaat tccgcacaaa aacgaccccg taatatacgg ggtcaataag
     6421 gacatggtat aaagggtatt atttcttcgc ttctacgcaa tcagtttcag aacgaattaa
     6481 aaatttctca gttgtttgcg gaatatgctg aatcagccag tcggccattt gcttttcctc
     6541 atttaaaatc gcttcgatga tcggaattga gactgtatca ccggcatttt ttgctgctgc
     6601 taatagtgag gtgtaacagg cgatttcaaa ttgctcgaag acatatccgc taatagagcc
     6661 tttaactatt tcatcagaag ggaatatacc accgattgac tgcccaagcg cagccatttt
     6721 actcatggaa tctttaatga ctgaacgtga aatgtcatta cgatcaagaa tagtttccag
     6781 ttgaacaatc tggtttttgg tttcactaag atgttgttca atacgagcgc gtagttcagg
     6841 ataattatct atacggctgg ccatggattc aagcatagat tcggcttgct tttccattgc
     6901 gtgggcgtca cgtaaccagt catgataatg ttcaatacga ttcatgaaaa tgtcctcttt
     6961 ataaaatgac taaaagttaa aattcatatt tcaggcttta ttttcggctt tcttatttac
     7021 gttattaagg gccagatcag tcagtttgat gtcggtggcc ttttcttctt ccagggtttc
     7081 tttcagaagc ttcgctgctt tacggtaacc taattgttca gccagcgtcg ctaatgtccc
     7141 gtaactggca atctcataat gctcgacttt ctgtgctgcg gcaatcagtg cggcatcacg
     7201 cacttcgttt ttctcggtac tttcgatgac ctcattagct tcttcaataa gaccttccat
     7261 tgccacacat ttcatgcgct taattttcag atttgattct gattccacaa cttgatcaat
     7321 tcgttcaatc tgtccatgag tttcctcgag gtgcgcatga aaagcctgac ttaatttttc
     7381 atttgatgtt gctcttgcga gttttgccag tgcccggggt aattgttttt ctgcgctgta
     7441 ggtatctgaa agcagttgaa taaatacatc ttcaatggtc ttcatattca tatttttctc
     7501 cagtgaaatc actgcgagcc atacggcccg cagatgcaat tatctttatc agctgatatt
     7561 aataattaac ggtgaatcag gatttgtctg atttattacc accgctttgt tgaccgcctt
     7621 ttttacccgc ttcagatgcg cgttgcggat catttttaaa attaccgccg ctatgctgac
     7681 cgcctttacg gcctgcgtcg gatgccttct cacggtcttc ggcgaaattt cctgaaccac
     7741 cacgatgttc ggccatgtta tttctcccgt tgcgttgcat tgtttcatta atatgagtgt
     7801 tgtgtgtcga cactcattaa aattagtcgc taatgagaat tagtcaaatt aagcgcaacg
     7861 agaagataga gggaaaatat attttgagga acattctgga tatattaaca attacctgag
     7921 gaataaatga cttagagaaa attattgatt ttactggtgt tatgttgcgg gattaatttg
     7981 tttgattata ttactgttgg gcggaaaaat gacgtaagtt gacgttcgac cggggtaagc
     8041 gaaacggtaa aaagataaat attaaatgaa tttaggattt ttccggcttc attaaagaaa
     8101 gttaaaatgc cgccagcgga actggcggct gtgggattaa ctgcgcgtcg ccgctttcat
     8161 cggttgtaca aaaactttca gtgccgccag cattttctct ggctcattaa tatgttgctc
     8221 gatgatttta acaatggccg aaccagaaat cgcgcccgca gctcctgcat caatcgctgc
     8281 ttttacctga tccggggcgg aaataccaaa cccctgcaat ggaggtgcag cgttgtactc
     8341 tttcagcttc gcaaccagat gattgagggg taacgcggcg cggttttctg cgccggtcac
     8401 gcccgctcgt gacagcaaat atgtgtaacc acgaccgtaa gaggctatct ggcgcagcag
     8461 gtcgtcatcg gcatttggcg ggcagatgaa gataggtgcg acattatggc gcaacgcggc
     8521 ctggcggaag ggcgcggact cttcaactgg cacatcggca accagcaccg aatcgacgcc
     8581 gactttctcg cactgggcat aaaactcatc aatgcctttg ttaaacacca gattggcata
     8641 catcaacagg ccaatgggaa tggtcgggtg tttctggcga atcagtgcca gcatttcaaa
     8701 acattgtgcc ggagtcacac ctgccgcaaa ggcgcgcaga gtggcgtttt gaatggtcgg
     8761 gccatccgcc agtgggtcgg agaaggggat acctaactcc agcgcgtcag caccggcttc
     8821 aattagcgta tcgataattt tcaatgactg ctcaatgccc ggatcaccga gcgtgacgaa
     8881 aggaatgaat gcgccttctt tgcgctcctt caactgggca aacagagatt cgtagcgttc
     8941 catcagattt cccctcgtgc tttcaaaata tcgtgaacgg tgaagatgtc tttatcgccg
     9001 cgaccggaaa ggttaaccac cagtagctgc tctttatccg ggttttcgcg catcattttc
     9061 agcgcatggg ccagggcgtg ggaggattcc agcgccggga tgatcccttc gtgcaggcac
     9121 agcgttttga aggcttcaag ggcttcatca tcggtaatag acacgtaatc agcgcgtcca
     9181 gtgctgttaa gatacgcgtg ttgtgggccg acagacggga aatccagtcc ggcggagatg
     9241 gagtaagatt cttcaatctg cccgtcttcg gtttgcatca tcggcgcttt cataccgaaa
     9301 taaatgccca cgcgaccatg ttttagcggt gcgccgtgct cgccagtttc gataccgtga
     9361 ccaccaggct ccacaccaat caggccgacg ttggtttcat tgatgaaatc agcaaacatg
     9421 ccgatggcat tcgaaccgcc gccaacacag gcgataacgg catccggcag gcgaccttct
     9481 ctttccagaa tctgcgcttt ggtttcttcg ccaatcatcc gctgaaactc acggacaatg
     9541 gtcggataag gatgcgggcc agccgcggtg cccagcatat agtgcgcggt ttcataactg
     9601 ccggaccagt cgcgtagcgc ctcattacag gcatctttca gcgtggcgga accgctatgt
     9661 accgggatca cttccgcacc cattaagcgc atacgaaaaa cgttaggcga ctggcgttca
     9721 acgtctttgg cacccatata aatacggcat ttcaggccga gcagggcgct ggcaagggcc
     9781 gacgccacgc catgctgacc ggccccggtt tcggcgatga tttcggtttt acccatccgc
     9841 ttcgccagca acgcctgccc cagcacctgg ttggttttat gtgcgccgcc gtgcagcaaa
     9901 tcttcacgct tgagatacag cgtggtgttc gtcccggctg taatgttctg gcatttggtc
     9961 agtgcggttg gacgcccggc atagtttttc agcaggtcgt tgaactgggc ttgaaattca
    10021 ggatcttttt gcgcgctgac aaaagcttct tccagctggc gcagagcagg catcaggatt
    10081 tgtggcacgt acatgccgcc aaactcacca aaataggggt taagtaatgt tgtcattgtt
    10141 cctttcctta atatgcgcgc agcgtctgga aaaccgaggc caaaagacgt gcgtctttga
    10201 tgcccggttg cgactctaca gcagaattaa aatcaagtcc ggcgcagccg gtttgtgccg
    10261 cttccacgca gttatctgcg cctaagcccc ccgccagcag aacgttgcca agcgattgac
    10321 catttaatag tgaccagtca aaacgttgcc cgcttccacc ctggccgttg tctaaaacat
    10381 atttatcaac gtgctgaaac tcgcgggcgg gcagggtttc accgacgctt aatgctttcc
    10441 agatggcaac atgtgctggc agagcttcac gcagcgtatc gatatacagc tgatcttcat
    10501 taccatgcag ttgcactgcc gccagcgata acaccttagc tttgtccacc acatcggcaa
    10561 tatcgtgatt gcggaacacg ccaacatact gcaacggtgc cgcagccatc acttcctgcg
    10621 cctgttcaac gttgacgcaa cgcggtgatg tcgcaacaaa aatcaaccca ccgtaaatcg
    10681 cgcccgcgtc ataagctgct ttagcatctt gcccacgcgt caggccacat actttattct
    10741 cacccagcaa cacccggcgc acggcggcgt gcaaatcgtc atgggccatc aacgccgaac
    10801 caatcagaaa accgttagcg aagtggctta actcgcgcac ctgagcgtaa gtattgatgc
    10861 cggattcgct gattaccgtt acgttgtgcc ccagtttcgg cgcaagctcg cgggtacggt
    10921 tgagatcaat cgacaaatca cgcagatcgc ggttgttgat gccaacgacc tttgccccta
    10981 atgcaatggc gcgctccagt tcctcttcat tactgacttc ggtcagcacc cccatctcca
    11041 gactgtgagc gacggctgca agctggcgat attggtcgtc atccagtact gaaagcatta
    11101 ataagcaggc atcggcctgg taatagcgcg ccagatagat ctggtaaggg tcgataatga
    11161 agtctttaca taaaatcggc tgcggggcga tttggctgac gatgaggagg aaatcaaagc
    11221 tcccctgaaa atatttctca tcagtcagca ccgaaattgc cgaagcgtaa tgtttataaa
    11281 tggcggcaat gcgtgccgga tcgaaatcat cacggatcac gccttttgac ggcgacgctt
    11341 ttttacactc cagaataaac gccgtgcgtg caccttgtag cgcatcataa aaatgtcgtg
    11401 tgctcggctg aacctcattc tgaaaactgg ccagcggttg ctgctgtttg cgggtttcta
    11461 cccaaatcgc cttgtctgcg acgattttcg ctaaaacggt ttgcatcatt taccctcgtg
    11521 ccgccagcgc ggtaactctg tcgtaagcgg aaccactgcg cagtacctca agaacggttt
    11581 gcgcattggc ttgcagatct tcatggccat gcaggcgcat taacatggcg acgttcgcag
    11641 cgacggctgc ttcatgggcg gcgtcgcctt taccttgtaa caagcgtgtt aaaatgtcac
    11701 ggttttcttc aggtgttccg cctgccagtt gctcctggtg gtagggagtc aggccaaaat
    11761 cttcagcggt caattgatag ctcttaattt tgccgtcatg cagctcggca acgattgtcg
    11821 gcgcgtgtaa tgaaacttca tccatcccgc cgctgtgcac caccgccgcg cgttgatacc
    11881 ccagcacgcg caaggtttcg gcaatcggca gcaccagttc cggactataa acaccaatta
    11941 acgccagcgg cggatgcgcc gggttaatca atggccccag cacattgaac agggtgcggg
    12001 ttttcagttg ctggcgaacc ggcatcgcgt ggcggaatcc ggtgtgatat ttcggcgcaa
    12061 agaggaaaca tacgcctaac tcgtccagcg cctggcgcga tttattggcg ttcatatcaa
    12121 gattaatacc gaatgccgcc agcagatccg acgagccgga tttactggag acgctgcggt
    12181 taccgtgttt cgccactttc agcccacagg ccgcggcgac aaacgcactg gcggtagaaa
    12241 tattgatact gttgctgccg tcaccgccag taccgacgat atcagcaaac agataatccg
    12301 ggcgcgggaa cggcgctgcg ttttccagta gcgcggttgc tgccccggcg atctcgttcg
    12361 ggtgctcacc acgaattttc atgctcacca gcgccgccgc cagttgttcc ggcttcagct
    12421 cgccacgcac caccgctgaa aacagctggt ggctttcttg ttggctaagc gtctgcgcct
    12481 gatacagttt ttccagaatc ggttgcagcg tgttggctgg ctctagtttc tgctgcgccc
    12541 aggccagcgt ttgttccagc aggcgagcgc cctgggtggt gagaatggat tccggatgga
    12601 actggaatcc acaaacgcga tccgcatcgt gacgtactgc catcaccatg ccattaaaat
    12661 gggcgttgat ggttaaaccg gccggaatgt tactgccaac cagcgagtga taacgcgcca
    12721 ccggcagcgg gtttgttaat ccggcaaaca tcgcctgacc gtcatgttca atgctcgacg
    12781 ctttaccgtg aagaatttcg cccgcctgac cgacatagcc cccgtaagct tcgacaattg
    12841 cctgatgtcc gaggcaaatg ccaataatgg gcagcttgcc acgcaagcgg gtgaggagtt
    12901 ccggcataca accggcttcg ctcggcacac cggggccagg agaaagcatc agtaccggat
    12961 tgctcatcgt cgccaggcgt tcaattaagg tctgcgccgg aatatggttg cggtaaatca
    13021 ccacgttatg accattgctg cgcaactgat ctgccaggtt gtacgtaaaa gagtcgatat
    13081 tatcgagcag cagaatgtca gccatcagaa agtctcctgt gcatgatgcg cggtggcaat
    13141 agcgcgcagt acagcgcggg ctttgttacg ggtttcgtcg gcttccgact gcggaacaga
    13201 atcaaggact acaccagcac ccgcttgcac ggtggcgata ccgttttcca ccagcgccga
    13261 gcggatcaca atgcaggtgt cgagatcgcc atgcgcggtg aaataaccta ccgcgccgcc
    13321 gtagctgccg cggcgacgac cttccgcctc ggcaattaac tgcatagcgc gtactttcgg
    13381 cgcaccgctt aacgtcccca tattcataca ggcgcgataa gcgtgcaggg cgtcaagatc
    13441 gtgacgcagt tcgccgacta cgcgagagac gaggtgcatc acataggaat aacggtcaac
    13501 tttggtgaga tcggcgacgt agcggctgcc aggggtgcaa atgcgtgcca gatcattacg
    13561 ggcgagatca accagcatca gatgttcaga cagctcttta tggtcagtac gcatttccag
    13621 ttcaatacgg ctgtcgagat ctctgtccag tgaaccatcg gcgcgacgac cgcgtgggcg
    13681 tgttccggca atcgggtaga tctcaatctg gcggctggtg gcatcatact tgagcgagct
    13741 ttccggcgac gcgccaaata gggtgaaatc attatcctgc ataaaaaaca tgtacgggct
    13801 gggattactc tttttcagca cgtaataggc cgccagcggt gacgggcagg gcagagagaa
    13861 acggcgagat ggcaccacct ggaaaatttc tccagcgcga atcgcttttt gcaacaaacg
    13921 cactacgcca ccgaactctt catcgctctg attacattca caacgcatat gcggcacgga
    13981 aaccactggc agcggcggcg cggcttcggt cagttgctga cgtagttcgt tcaggcgagc
    14041 agtgagacgt tgtttttctt cttcattcgg agcaaacagg ctggcctgaa tacgggtgct
    14101 ttttttctga tggtcaatca ccatcagcgt ttcagcgaga taaaaacaga aatcagggca
    14161 gttattttcc gctgacagtt gcggtaaatc ttcaaatccc gccacaaggt cataagagaa
    14221 caggccgccg aagaacattg cttctcgttc ttccttcggt acattcaaca gattctgcaa
    14281 taaacggaaa gcgtcaaaaa ccgaaaggga gcataagcgg gcgtcttcat ccagcagtgg
    14341 actgacaggg gggaagcgca gcacacggca gtttggtgat tgttcacttt ccacacccgc
    14401 aggcagggcg ttatccagta gtgccagcag ggcttcgcca ttgccggaaa gtgcctgaat
    14461 tgtgacggtg tcacctaatg ctgtaatgtg cagcgcattg tctatcagca gcaggctttt
    14521 taaatcatct ttgctgtcga tatctgcgga ttccagcagc agcgttgccg gacgattccc
    14581 acacaactgg tgaaaaagcg cagtcgggtt gtcgcgataa gcgccttcgc aggttagcag
    14641 ttcgagagtc ggtttttgtg tttgcattgt tattctctaa ttttgttcaa aaaaaagccc
    14701 gctcattagg cgggctgggt atctgattgc tttacgcatg gtgaatacac tgcccgtttc
    14761 aggaagtggc caccaacctt tcagtacgaa aattgctttc attgtcgata ccatttttac
    14821 gtgaacttgc gtactagtca actggttcga tgattaattg tcaacagctc atttcagaat
    14881 atttgccaga cccgttatga tgtcggcgca aaaaacatta tccagaacgg gagtgcgcct
    14941 tgagcgacac gaattatgca gtgatttacg acctgcacag ccataccaca gcttccgatg
    15001 gctgcctgac gccagaagca ttggtgcacc gtgcagtcga gatgcgcgtc ggcaccctgg
    15061 cgatcaccga ccatgacacc acagctgcaa tcgcgccagc aagagaggaa atttcacgtt
    15121 cgggactggc gctgaatctt attcccggcg tggaaatttc cacggtctgg gaaaatcatg
    15181 aaattcatat tgtcgggctg aatattgata ttactcatcc gctaatgtgt gagttcctcg
    15241 cgcagcagac agaacggcgc aatcagcggg cgcagcaaat tgccgaaaga cttgaaaaag
    15301 cgcaaatacc tggggcgctg gaaggcgcac aacgactggc gcagggcggc gcagtgacgc
    15361 gcggtcattt tgcgcgtttc cttgtggagt gcggcaaagc cagttcaatg gcagatgtct
    15421 ttaaaaagta tctggcgcgc gggaaaaccg gatacgttcc gccacagtgg tgtacaatag
    15481 aacaagctat tgatgtcatt catcattctg gcggtaaggc ggtgctagct catcctgggc
    15541 ggtacaatct tagtgctaaa tggctgaaaa gattggttgc gcattttgcc gaacaccacg
    15601 gcgacgcgat ggaagtcgcg cagtgccagc aatcgcccaa tgaacgcacc cagctggcca
    15661 cacttgcgcg tcagcatcat ttatgggcat cacaaggatc tgattttcat cagccatgcc
    15721 cgtggatcga actgggtcgt aaactctggc tgcccgcagg cgttgaaggc gtctggcagc
    15781 tatgggaaca gccgcagaac accacagaga gggaattatg agccagtttt tttatattca
    15841 tcctgataac ccgcagcaac gtctgatcaa ccaggcggtg gagatcgtgc gtaaaggcgg
    15901 ggtgattgtc tatccaactg attccggcta tgcgctcggc tgtaaaattg aagataaaaa
    15961 cgccatggag cgtatttgtc gtattcgcca gctgccggac ggtcacaact ttaccctgat
    16021 gtgtcgcgat ctttctgaac tctcgaccta ttcattcgtg gataacgttg cgtttcgttt
    16081 gatgaaaaac aacacgccgg gcaactatac cttcatcctg aaggggacga aggaagtgcc
    16141 acgccgtctg ttgcaggaga aacgcaaaac catcggtatg cgtgtgcctt ctaacccgat
    16201 tgcgcaggcg ttgcttgaag cactgggcga accgatgctt tccacttcgc taatgctgcc
    16261 aggcagcgaa tttaccgaat ccgatcccga agaaattaaa gaccgtctgg aaaaacaggt
    16321 agatttgatt attcacggcg gctatctcgg gcagaaacca acaacggtta ttgatcttac
    16381 cgatgatacg ccggtcgtgg tgcgtgaagg cgtaggtgat gtgaagcctt tcttataagt
    16441 gcgtaactct gtatactacg cggccttaaa atggcgctat acggcgccat tgattctcct
    16501 gttcgacgcc tgtgaaggcg acaccaaaag gaagctctat gagcgaaaaa ttacagaaag
    16561 tgctggcccg cgctggccac ggctcccgcc gtgaaatcga atctattatt gaagccggtc
    16621 gtgtgagtgt tgatggcaaa attgctaaac tcggcgatcg cgttgaagtt acccctgggc
    16681 tgaaaattcg tatcgatggt catctgattt cggtacgtga gtccgctgaa caaatttgtc
    16741 gcgttctggc ctattacaaa ccggaaggtg agttgtgtac ccgtaacgat ccggaagggc
    16801 gtccgacggt gtttgaccgt ctgccaaagc tgcgtggcgc acgctggatt gccgtaggtc
    16861 gtctggacgt taacacctgt ggtctgctgc tgttcaccac cgatggtgaa ctggcaaacc
    16921 gtttaatgca tccaagccgt gaagttgaac gtgaatacgc cgtgcgtgta tttggtcagg
    16981 ttgacgacgc gaaactgcgt gatttgagtc gtggcgtgca actggaagat ggtccggcag
    17041 cttttaaaac catcaagttc agcggcggcg aagggatcaa ccagtggtac aacgtgactc
    17101 tgaccgaagg ccgtaaccgt gaagttcgtc gtctgtggga agcggttggt gtgcaggtga
    17161 gccgcctgat ccgtgttcgt tacggtgata tcccactgcc aaaaggtctg ccacgcggtg
    17221 gctggacgga gctggacctc gcccagacta actatctgcg cgaactggtg gagcttccgc
    17281 cagaaaccag ctctaaagtc gctgtagaaa aagaccgtcg tcgcatgaag gctaatcaga
    17341 ttcgtcgtgc ggtgaaacgt cacagtcagg tgagcggcgg tcgtcgttct ggcgggcgta
    17401 ataacggtta atcaaagtat gccggacgtc atatccggca tttttacaga ttaataatca
    17461 atccctattt gcgccttaac accggcatca aacgcatgtc tgatggggcg cagctcactt
    17521 accgtatccg ccagttcaag aatatcccga tgacagcctc gacccgtgat aatcaccgtc
    17581 tgttgatgtg gacgttcatt taacgcctgc accacttctt ccagtggcaa atagtcatac
    17641 gccaccatat acgtcagttc atcaagcaaa accatatcca gtgaggaatc agcaagcatc
    17701 cgctttgcat gttgccagac ttcgcggcag gcggcggtat cagactcgcg gttttgtgta
    17761 tcccaggtaa agcccgttgc catcacctga aactcaacgc catgtggctc cagcagattg
    17821 cgttcgccat taggccaggt gcctttaata aactgcacga cgcccacttt ttttccgtga
    17881 ccaactgcgc gtgttgccgt accaaatgcc gcggtggttt tgccttttcc attgccggta
    17941 aagacgatga taataccgcg ttcatcctgg gcctgggcca cacgagcatc tactttttct
    18001 ttcactcgct gctgacgctg ttggtagcgt tcatcactca ttgggaaatt cctggtttac
    18061 ggcccggttg ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc
    18121 agaggtagag cggcatgata tcagcgggtg ttttaagttt ctgtggatct tcggtcggga
    18181 aggcgcttgc gcgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg acgcgcaggc
    18241 gctgctgata ttcatcggcc agtacctgca tcatcccttc ggtggcaaat ttcgacgctg
    18301 catatgcacc ccagttggct cgtccctgac gtccaacgct tgatgaagta aagaccagtg
    18361 aaccggcgtc cgatttgagt aataaaggaa gcagtgcctg ggtgagcata aaggtggcat
    18421 taacgttgac ctgcatgacg tcctgccaga cctgcggatt ttgttcgctc attgggcaaa
    18481 catcgccgag caatccggca ttatgcaaaa caccatccag acgcggataa ttaacgacaa
    18541 tgcgctgtgc cagttgttgg caattttcgg acgtgcaggt cagcaaatcg agaataaacc
    18601 actgtggctg acgcccagtt tcttcgttta tgtggctggc tacctgacgt aatttttctt
    18661 cattacggcc caacagaatc actgtcgcac catagcgtgc atacgtcatc gcggcttcac
    18721 gaccaatccc atcgctggct cccgtcacca ggataatgcg atcattgagt aaatcttgtt
    18781 ttggctggta atgcatggct actcctcaac gacgttgtct gtcgcgctgc cagtgtactt
    18841 tatgactttg gggctttatg cctgaaacag aagggcattt caatcagcct ggggcgataa
    18901 caccgcaaaa tggatacttt gtcatacttt cgctgcaata acatctctgc gagacggctt
    18961 aacatgcctg ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga
    19021 attgttgtct gaatatggtt tgtttttggc taaaatcgtt accgttgtgc tagcgattgc
    19081 ggcgattgcc gccattattg tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt
    19141 acgggtcaac aatctcagcg aacagtataa ggagacgaaa gaagaactgg ccgcggcgct
    19201 gatggactca catcagcaaa aacagtggca caaagcgcag aagaaaaagc acaagcaaga
    19261 agcgaaagca gcaaaagcga aagccaaact gggcgaggtg gcaactgaca gtaaacctcg
    19321 cgtctgggtg ctggatttta aaggcagcat ggacgcccat gaagtgaact cgctacgtga
    19381 agagataacg gcggtactcg cagcattcaa accgcaggat caggttgtgc tccgtctgga
    19441 aagccctggt ggcatggtgc atggttacgg gttggcggct tcgcagctgc agcgtctgcg
    19501 tgataaaaac attcctttaa ctgttacggt agacaaagtc gctgccagcg gcggttacat
    19561 gatggcctgt gtggcggaca aaattgtttc cgcaccgttt gctattgtgg gttccattgg
    19621 ggtggtggcg caaatgccca actttaaccg cttcctgaaa agcaaagata ttgatatcga
    19681 actgcacacc gccgggcagt ataagcgcac gctgaccttg ctgggtgaaa ataccgaaga
    19741 agggcgggag aaattccgcg aagagctgaa cgaaacgcat cagttattta aagattttgt
    19801 gaagcgtatg cgtccgtctc tggatattga acaggtggca acgggtgaac actggtacgg
    19861 acaacaggcg gtagagaaag gcctggttga tgaaatcaac accagtgatg aagttattct
    19921 tagcctgatg gaaggccgtg aagtggttaa tgtacgctat atgcagcgta aacgactcat
    19981 tgaccgattc accggcagcg cggcagagag cgccgatcga ttgttgctac gctggtggca
    20041 gcggggtcaa aagccattga tgtaaaagac aaacgcgagg ctaagacctc gcgttttgct
    20101 ttaatcaacc agatgatatt tttctgaaag cacatgggcc aggtgtttga acatattaaa
    20161 caccgcggtg cttttggctg ttggcaatcc ttgttcatct aaaaagtagt cgccggtaaa
    20221 taccagcacg ccattacgct gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc
    20281 atgctcacga atgattttgt tagcctcttt caacagcgtt tcgcggtcga tgggttgtgt
    20341 ctctttgttc attatttact ccttaaacaa ggacattagt ctacgccagg catggcttgc
    20401 agacaaatat accacgctgg tggcaagagc gccttactgg caactttgga ttttgcatgc
    20461 taataaagtt gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc aatctggcaa
    20521 tagatttgct tgacattcga ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc
    20581 tgttaactca gtcacctgaa ttttcgtgaa cagagtcacg acaagggggt gatatccgca
    20641 gagagcgagt ccatatcggt aactcgttgc cagtggaagg tttatcaacg tgcgacgcat
    20701 tcctggaaga atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc
    20761 cccggcaaaa gccaaaacga tcaacaagta tctgggtagt gactacgtgg tgaaatccag
    20821 cgtcggtcac atccgcgatt tgccgaccag tggctcagct gccaaaaaga gtgccgactc
    20881 tacctccacc aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg
    20941 tatgggggtt gacccgtggc acaattggga ggcgcactat gaagtgttgc ctagtaaaga
    21001 gaaggtcgtc tctgaactga aacaactggc tgaaaaagcc gaccacatct atctcgcaac
    21061 cgaccttgac cgcgaagggg aagccattgc atggcacctg cgggaagtga ttggtggtga
    21121 tgatgcgcgc tatagccgag tggtgtttaa cgaaattact aaaaacgcga tccgccaggc
    21181 atttaacaaa ccgggtgagc tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt
    21241 tatggaccgc gtggtggggt atatggtttc gccgctgctg tggaaaaaga tcgctcgtgg
    21301 tctgtctgcc ggtcgtgtgc agtcggtggc ggtccgcctg gtggtagagc gtgagcgtga
    21361 aattaaagcg ttcgtaccgg aagagttctg ggaagtcgat gccagcacga ctacgccatc
    21421 tggtgaagcg ttggcgttgc aggtgactca tcagaatgac aaaccgttcc gtccggtcaa
    21481 caaagaacaa actcaggctg cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga
    21541 acgtgaagac aaaccgacaa ccagtaaacc tggcgctcct tttattacct ctacgctgca
    21601 acaagctgcc agcacccgtc ttggttttgg cgtgaaaaaa accatgatga tggcgcagcg
    21661 tttgtatgaa gcaggctata tcacttacat gcgtaccgac tccactaacc tgagtcagga
    21721 cgcggtaaat atggttcgcg gttatatcag cgataatttt ggtaagaaat atctgccgga
    21781 aagtccgaat cagtacgcca gcaaagaaaa ctcacaggaa gcgcacgaag cgattcgtcc
    21841 ttctgacgtc aatgtgatgg cggaatcgct gaaggatatg gaagcagatg cgcagaaact
    21901 gtaccagcta atctggcgtc agttcgttgc ctgccagatg accccagcga aatatgactc
    21961 cacgacgctg accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg
    22021 ctttgatggc tggacaaaag tgatgcctgc actgcgtaaa ggcgatgaag atcgtatctt
    22081 accggcagtc gataaaggcg atgctctgac gctcgttgaa ctaacaccag cccagcactt
    22141 taccaagccg ccagcccgtt tcagtgaagc atcgctggtt aaagaactgg aaaaacgtgg
    22201 tatcggtcgt ccgtctacct atgcgtcgat catttcgacc attcaggatc gtggctatgt
    22261 gcgagtagaa aatcgtcgtt tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct
    22321 tgaagaaaat ttccgcgagt taatgaacta cgactttacc gcgcagatgg aaaacagcct
    22381 cgaccaggtg gcaaatcacg aagcagagtg gaaagctgta ctggatcact tcttctcgga
    22441 tttcacccag cagttagata aagctgaaaa agatccggaa gagggtggta tgcgcccgaa
    22501 ccagatggtt ctgaccagca ttgactgccc gacttgtggt cgcaaaatgg ggattcgcac
    22561 agcgagcacc ggggtattcc ttggctgttc tggctatgcg ctgccgccga aagagcgttg
    22621 caaaaccacc attaacctgg tgccggaaaa cgaagtgctg aacgtgctgg aaggcgaaga
    22681 tgctgaaacc aacgcgctgc gcgcaaaacg tcgttgcccg aaatgcggca cggcgatgga
    22741 cagctatctc attgatccga aacgtaagtt gcatgtctgt ggtaataacc caacctgcga
    22801 cggttacgag atcgaagagg gcgaattccg cattaaaggt tatgacggcc cgatcgttga
    22861 gtgtgaaaaa tgtggctctg aaatgcacct gaaaatgggg cgtttcggta aatacatggc
    22921 ctgcaccaac gaagagtgta aaaacacacg taagatttta cgtaacggcg aagtggcacc
    22981 accgaaagaa gatccggtgc cattacctga gctgccgtgc gaaaaatcag atgcttattt
    23041 cgtgctgcgt gacggtgctg ccggtgtgtt cctggctgcc aacactttcc cgaaatcgcg
    23101 tgaaacgcgt gcgccactgg tggaagagct ttatcgcttc cgcgaccgtc tgccggaaaa
    23161 actgcgttat ctggccgatg cgccacagca ggatccggaa ggtaataaga ccatggttcg
    23221 ctttagccgt aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa aggcgactgg
    23281 ctggtcagca ttttatgttg atggcaaatg ggttgaagga aaaaaataac ctttaattct
    23341 atcaggtttt tataaacaaa gggtcgcgaa agcggccctt ttttattgca tattattttt
    23401 tcttcacacc tatacactaa ggctataaat gatatagtgg ttatagttag cacctttttt
    23461 attattaaat cgtattagtc acccgccagg tgtgacgaaa aaacgatgtt ctgatggcgt
    23521 ctaagtggat ggtttaacat gaaattacaa caacttcgct atattgttga ggtggtcaat
    23581 cataacctga atgtctcatc aacagcggaa ggactttaca catcacaacc cgggatcagt
    23641 aaacaagtca gaatgctgga agacgagcta ggcattcaaa ttttttcccg aagcggcaag
    23701 cacctgacgc aggtaacgcc agcaggacaa gaaataattc gtatcgctcg cgaagtcctg
    23761 tcgaaagtcg atgccataaa atcggttgcc ggtgagcaca cctggccgga taaaggctcg
    23821 ctgtatatcg ccaccacgca tacccaggca cgctacgcgt taccaaacgt tatcaaaggc
    23881 tttattgagc gttatccccg cgtttctttg catatgcacc agggcccgcc gacacaaatt
    23941 gctgatgccg tctctaaagg caatgccgat ttcgctatcg ccacagaagc gctgcatctg
    24001 tatgaagatt tagtgatgtt accgtgctac cactggaatc gggctattgt agtcactccg
    24061 gatcacccgc tggcaggcaa aaaagccatt accattgaag aactggcgca atatccgttg
    24121 gtgacatata ccttcggctt taccggacgt tcagaactgg atactgcctt taaccgcgca
    24181 gggttaacgc cgcgtatcgt tttcacggca acggatgctg acgtcattaa aacttacgtc
    24241 cggttagggc tgggggtagg ggtcattgcc agcatggcgg tggatccggt cgccgatccc
    24301 gaccttgtgc gcgttgatgc tcacgatatc ttcagccaca gtacaaccaa aattggtttt
    24361 cgccgtagta cttttttgcg cagttatatg tatgatttca ttcagcgttt tgcaccgcat
    24421 ttaacgcgtg atgtcgttga tgcggctgtc gcattgcgct ccaatgaaga aattgaggtc
    24481 atgtttaaag atataaaact gccggaaaaa taatttcttg cgttattttc ggcacctttt
    24541 atgtagcgaa ggtgccggaa tatattctct tttgttactt atttctatac cgtgcacaat
    24601 atttttatcc ctcatattta tagggtaaat tacctgtaaa gtagtgtcta attacccgcc
    24661 tcgcaaattc cagttttcaa atatctattt caggtcaaaa gattgaatta ttcactggag
    24721 acgattcgta aattcgctgg atttttgact aaagtttctt taggatttgt ctcatcgtat
    24781 gattaattac accaatcatt caacgtcttg ctgataagtg atggcgatta tatgaggtta
    24841 gcaatgcctt ctggaaatca ggaaccccgc cgtgatcctg aattaaaacg taaagcctgg
    24901 ctgacggttt ttcttggttc tgcacttttc tgggtggttg tcgcactgct gatttggaaa
    24961 gtgtggggat aactatggtc ggtcaggagc aactggagtc gtcaccatta tgccagcata
    25021 gtgacaatga accagaaacg aaacgggaat gttccgtcgt tattccagac gactggcaac
    25081 taacatcgca gcagcaagcc tttatagaac tgtttgctga agatgatcag ccgaaacaat
    25141 aattatcatc attcttatta cccattttta atgaattaaa gggcttttaa tacaccgcag
    25201 caataacagc ttgagttatc tcaacacaaa ataataaccg ttaagggtgt agcctatgat
    25261 caacacaaat atgaaatatt ggtcctggat gggcgcgttt tctctgtcga tgctcttctg
    25321 ggccgaactc ctctggatca ttactcactg atccttgacc ccgctgcggc ggggttgtca
    25381 tttgctttgc cacaaggttt ctcctctttt atcaatttgg gttgttatca aatcgttacg
    25441 cgatgtttgt gttatcttta atatttaccc tgaagagaat cagggcttcg caaccctgtc
    25501 attaaggagg agctatgtcg tcaaccctac gagaagccag taaggacacg ttgcaggcca
    25561 aagataaaac ttaccactac tacagcctgc cgcttgctgc taaatcactg ggcgatatca
    25621 cccgtctacc caagtcactc aaagttttgc tcgaaaacct gctgcgctgg caggatggta
    25681 actcagttac cgaagaggat atccacgcac tggcgggatg gctgaaaaat gcccatgctg
    25741 accgtgaaat tgcctaccgc ccggcaaggg tgctgatgca ggactttacc ggcgtacctg
    25801 ccgttgttga tctggcggca atgcgcgaag cggttaaacg cctcggtggc gatactgcaa
    25861 aggttaaccc gctctcaccg gtcgacctgg tcattgacca ctcggtgacc gtcgatcgtt
    25921 ttggtgatga tgaggcattt gaagaaaacg tacgcctgga aatggagcgc aaccacgaac
    25981 gttatgtgtt cctgaaatgg ggaaagcaag cgttcagtcg gtttagcgtc gtgccgccag
    26041 gcacaggcat ttgccatcag gttaacctcg aatatctcgg caaagcagtg tggagcgaat
    26101 tgcaggacgg tgaatggatt gcttatccgg atacactcgt tggtactgac tcgcacacca
    26161 ctatgatcaa cggccttggc gtgctggggt ggggcgtggg tgggatcgaa gcagaagccg
    26221 ccatgttagg ccagccggtt tccatgctta tcccggacgt agtgggcttt aaacttaccg
    26281 gaaaattacg tgaaggtatt accgccacag acctggttct cactgttacc caaatgctgc
    26341 gcaaacatgg cgtggtgggg aaattcgtcg aattttatgg tgatggtctg gattcactac
    26401 cgttggcgga tcgcgccacc attgccaata tgtcgccaga atatggtgcc acctgtggct
    26461 tcttcccaat cgatgcagta accctcgatt acatgcgttt aagcgggcgc agcgaagatc
    26521 aggtcgagtt ggtcgaaaaa tatgccaaag cgcagggcat gtggcgtaac ccgggcgatg
    26581 aaccgatttt taccagtatg ttagaactgg atatgaatga cgttgaagcg agcctggcag
    26641 ggccaaaacg cccacaggat cgcgttgcac tgcccgatgt accaaaagca tttgccgcca
    26701 gtaacgaact ggaagtgaat gccacgcata aagatcgcct gccagtcgat tatgttatga
    26761 acggacatca gtatcagtta cctgatggcg ctgtggtcat tgctgcgata acctcgtgca
    26821 ccaacacctc taacccaagt gtgctgatgg ccgcaggctt gctggcgaaa aaagccgtaa
    26881 ctcttggcct caagcggcaa ccgtgggtca aagcgtcgct ggcaccgggt tcgaaagtcg
    26941 tttctgatta tctggcaaaa gcgaaactga caccgtatct cgacgaactg gggtttaacc
    27001 ttgtgggata cggttgtacc acctgtattg gtaactctgg gccgctgccc gatcctatcg
    27061 aaacggcaat caaaaaaggc gatttaaccg tcggtgcggt gctgtccggc aaccgtaact
    27121 ttgaaggccg tatccatccg ctggttaaaa ctaactggct ggcctcgccg ccgctggtgg
    27181 ttgcctatgc gctggcggga aatatgaata tcaacctggc ttctgagcct atcggccatg
    27241 atcgcaaagg cgatccggtt tatctgaaag atatctggcc atcggcacaa gaaattgccc
    27301 gtgcggtaga acaagtctcc acagaaatgt tccgcaaaga gtacgcagaa gtttttgaag
    27361 gcacagcaga gtggaaggaa attaacgtca cacgatccga tacctacggt tggcaggagg
    27421 actcaaccta tattcgctta tcgcctttct ttgatgaaat gcaggcaaca ccagcaccag
    27481 tggaagatat tcacggtgcg cggatcctcg caatgctggg ggattcagtc accactgacc
    27541 atatctctcc ggcgggcagt attaagcccg acagcccagc gggtcgatat ctacaaggtc
    27601 ggggtgttga gcgaaaagac tttaactcct acggttcgcg gcgtggtaac catgaagtga
    27661 tgatgcgcgg caccttcgcc aatattcgca tccgtaatga aatggtgcct ggcgttgaag
    27721 gggggatgac gcggcattta cctgacagcg acgtagtctc tatttatgat gctgcgatgc
    27781 gctataagca ggagcaaacg ccgctggcgg tgattgccgg gaaagagtat ggatcaggct
    27841 caagccgtga ctgggcggca aaaggacctc gtctgcttgg aattcgtgtg gtgattgccg
    27901 aatcgtttga acgaattcac cgttcgaatt taattggcat gggcatcctg ccgctggaat
    27961 ttccgcaagg cgtgacgcgt aaaacgttag ggctaaccgg ggaagagaag attgatattg
    28021 gcgatctgca aaatctacaa cctggcgcga cggttccggt gacgcttacg cgcgcggatg
    28081 gtagccagga agtcgtaccc tgccgttgtc gtatcgacac cgcgacggag ttgacctact
    28141 accagaacga cggcattttg cattatgtca tccgtaatat gttgaagtaa caagtatttg
    28201 cttgccggtt attttgcttc cggcaagcaa atgaattaca caatgcaaga gggttatttg
    28261 ttcagcaaat ggcccatttt ctcggctttg gtatcgagat aatgttcgtt attggggtta
    28321 cgacctacaa tcaatggtac gcgttcaaca atattaatcc ctgcttcggt cagaatttcg
    28381 acttttttcg ggttattggt taacaagcgg acttcattga cgccaaggag tttgaacata
    28441 tcagcgcaaa gagtgaagtc gcgctcatca gcggcgaagc ctaactggtg gttagcctct
    28501 acggtatcgt aaccttgatc ctgcagtgcg taagcgcgga ttttattcag cagaccaatg
    28561 ttacgacctt cctgacggtg atacagcaaa ataccacggc cttcctcggc aatttgcgtc
    28621 aatgccgctt cgagctggaa gccacaatcg cagcgcaagc tgaacagggc gtcaccggtc
    28681 agacattcgg aatggacgcg cgcaagtacc ggggtatgcc cggaaatatc ggcatagact
    28741 agcgcgacat gatcgtgtcc ggttgccagt tcttcaaatc ccaccatcag gaaatcgccc
    28801 catggggttg gcagtttggc ttctgccaca cgtttaagct gcatgaaatt ctccagataa
    28861 tgctggttct gtattggctt attttgccat aacgagaagg ggttcaccta atcacgacgc
    28921 gtcgatcgtt cacggaatgg cacaaatctg tcaatttttc ttggaactgg cattttcagt
    28981 tatgattgtg ggacttatca aaaaggagag gccatgcgtt cgattgcccg acgtaccgca
    29041 gtgggagctg cactattgtt tgtcatgcca gtagccgtat ggatttctgg ctggcgttgg
    29101 caacctggag aacaaagttg gcgactaaaa gcggcttttt gggttactga aactgtcacc
    29161 cagccctggg gcgtcattac acatttgatt ttgttcggct ggtttctctg gtgtctgcgt
    29221 tttcgcatta aggctgcctt tgtattattt tccattctgg cggcggcaat ccttgttgga
    29281 caaggcgtta aatcctggat caaagacaaa gtacaggaac cacgaccttt tgttatctgg
    29341 ttggaaaaaa cacatcatat tccggttgat gagttctaca ctttaaagcg agcagaacgc
    29401 ggaaatctag taaaagaaca gttggctgaa gagaaaaata ttccacaata tttgcgttca
    29461 cactggcaga aagagacggg gtttgccttt ccttccggtc acacgatgtt tgctgccagt
    29521 tgggcactgc tggccgttgg tttgctgtgg ccgcgtcggc gaacgttaac cattgctatc
    29581 ttgttggtct gggcaacggg agtcatggga agccgcctgc tactcgggat gcattggcca
    29641 cgcgatctgg tggtagctac gttgatttcg tggacgctgg tggcggtggc aacgtggctt
    29701 gcgcaacgaa tttgtgggcc attaacacca cctgcggaag aaaatcgcga aattgcacaa
    29761 cgagaacaag aaagttaacg ctggttgatt ttccgaattt aggccttaaa tcatcaacaa
    29821 tgcgcgtgga tgccatttcg cagacggcgc gaaaatggta ctttaaaggg ctattgcggt
    29881 aagttgacca taatttattc gctctaacca cataacggga agtaatgtga aatatttact
    29941 cattttctta ctggtgttag cgatcttcgt gatttcggtc acgttgggtg cgcagaacga
    30001 tcaacaggtg acgtttaatt atctgttagc gcaaggggag taccgtattt ccacattgct
    30061 ggcggtattg tttgctgcgg ggtttgctat cggttggttg atttgtggcc tgttctggct
    30121 gcgagttcgt gtttccctgg cgcgcgctga acgtaaaata aagcgactgg aaaaccagct
    30181 ttcacccgcg actgacgtgg ctgtagtgcc gcactcgtca gcggcgaagg aataactttc
    30241 tatgctggag ttgttgtttc tgcttttgcc tgtagccgct gcctatggct ggtatatggg
    30301 ccgcagaagt gcgcaacaaa acaagcaaga tgaagccaac cgcttgtcgc gtgattacgt
    30361 agcgggggtt aacttcctgc ttagtaatca acaggataaa gcggtagacc tgtttctcga
    30421 tatgcttaaa gaggatacag gcaccgttga agcccacctt acgctcggaa acctgttccg
    30481 ttcgcgtggc gaagttgatc gcgctattcg catccatcag accctaatgg aaagcgcctc
    30541 gctgacctat gaacagcgtc tgttggcgat tcaacaactg gggcgtgatt acatggccgc
    30601 cgggttatac gaccgcgcgg aagacatgtt caatcagctg accgatgaaa ctgacttccg
    30661 cattggcgcg ctgcaacagt tgctacaaat ctaccaggct accagtgagt ggcagaaagc
    30721 aattgatgtt gccgaacgcc tggtgaagct gggtaaagat aaacagcgcg tcgaaattgc
    30781 ccatttctac tgtgagttag ccctgcagca tatggccagc gacgatctcg atcgtgccat
    30841 gaccttgcta aaaaaagggg cggcggcaga taaaaacagc gcccgcgtat ccatcatgat
    30901 gggacgcgtg tttatggcga aaggagaata cgccaaagcc gtcgaaagtt tgcaacgcgt
    30961 catatcccag gacagagaac tggtcagcga aacgctggaa atgttgcaaa cctgctatca
    31021 gcagttgggt aaaactgccg aatgggcaga attcctgcaa cgcgcggtgg aagagaacac
    31081 cggtgccgat gctgaattga tgcttgctga tatcatcgaa gcgcgcgacg gtagtgaggc
    31141 tgcacaggtc tatattacac gccagcttca gcgtcatccg accatgcgtg tattccataa
    31201 gctaatggat taccacttaa atgaagcgga agaagggcgc gccaaagaga gtctgatggt
    31261 gctgcgtgac atggttggcg agaaggtgcg tagtaagcct cgttatagct gccagaaatg
    31321 tggttttacc gcatacaccc tctactggca ttgtccgtct tgtcgtgcct ggtcaactat
    31381 taaaccgatt cgcggtcttg atggcctgta atttttaaaa aaatccgact ttagttacaa
    31441 catactagtt attgatgtcc cattgtgctc cggcaacgac ggagcagaaa aagcctgcta
    31501 ggggaacaat cgcaactgtt aattttttat ttccacgggt agaatgctcg ccgtttacct
    31561 gtttcgcgcc acttccggtg cccatcatca agaaggtctg gtcatgacgt taactgcttc
    31621 atcttcttcc cgcgctgtta cgaattctcc tgtggttgtt gcccttgatt atcataattg
    31681 tgatgacgcg ctgtcctttg tcgacaagat cgacccacgc gattgtcgtc tgaaggtcgg
    31741 taaagagatg tttacattgt ttgggccaca gtttgtgcgc gaacttcaac agcgtggttt
    31801 tgatatcttt cttgacctga aattccacga tatccccaac actgcagcgc acgctgtcgc
    31861 tgctgcagct gacttaggcg tgtggatggt gaatgctcat gcctccggtg gggcgcgtat
    31921 gatgaccgca gcgcgtgagg cactggttcc gtttggcaaa gatgcaccgc ttttgattgc
    31981 tgtgacagtg ttgaccagca tggaagccag cgacctggcc gatcttggcg tgacactgtc
    32041 acctgcagat tatgcagaac gtctggcagc actgacgcaa aaatgtggcc ttgatggtgt
    32101 ggtgtgttct gctcaggaag ctgtgcgctt taaacaggta ttcggtcagg agttcaaact
    32161 ggttacaccg ggcatccgtc cgcaggggag tgaagctggt gaccagcgcc gcattatgac
    32221 gccagaacag gcgttggcgg ctggtgttga ttatatggtg attggtcgcc cggtaacgca
    32281 atcggtagat ccagcgcaga cactgaaagc gatcaacgcc tctttacagc ggagtgcatg
    32341 atgagtgatt ccaacagccg tctggtctac tcaacggaaa ccgggcgtat tgatgagccc
    32401 aaagcggccc cagtacgccc taaaggcgat ggagcggtgc gtattcagcg tcagaccagt
    32461 ggacgtaaag gtaagggcgt ttgcctgatt accggtatcg atctcgatga tgccgaactg
    32521 acaaaacttg cagcggaact gaagaaaaaa tgcggctgcg gcggagcagt taaagatgga
    32581 gttattgaaa tccagggcga taagcgtgat ttattaaaat cactacttga agcgaaaggg
    32641 atgaaggtaa aactcgcagg cggttaacat aaaaagccac ggatatatcc gtggctttcg
    32701 aatattttac tgtgtgtatt attcagttat ttcgtactga acagaatcaa gcgtattata
    32761 attgcgagtc gataatcgac atttatttac gattatttac cgacctggtg actaataaca
    32821 ccaccgacag ctgcaccacc taatgtaccc aacgtactgc catcggtcag tactgcaccg
    32881 cctaatgccc ctgcacccgc gccgattgcg gtgttgcggt cccgtttaga ccagttagaa
    32941 caggcactca gagacattgc caaagtaatt gccagaacag ccgcggtcat ttttttgctc
    33001 gttacaaaca taatactctc tcctgaattt atgattcacg gaagtaagct ctctataact
    33061 ataatagcta agaataagcc tggtgaaatt attccgtgaa atctgctcgc gcgcagggtt
    33121 atatcacg
//
LOCUS       DAJTNM010000029        33007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTNM010000029 DAJTNM010000000
VERSION     DAJTNM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.029"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            17..4549
                     /locus_tag="OO172_001520"
     CDS             17..4549
                     /locus_tag="OO172_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR5511751.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDVWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSS
                     SDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTP
                     DDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIA
                     DALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDKTANGGAGAYNAS
                     HDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEEN
                     GAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALG
                     SSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEA
                     HDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIG
                     LNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEF
                     SVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVT
                     NIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAE
                     EENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNIT
                     LGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQRMVEMDNKLSKTESKL
                     SGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGST
                     NSQGEYSAALGAGIQW"
     gene            4917..6572
                     /gene="lldP"
                     /locus_tag="OO172_001521"
     CDS             4917..6572
                     /gene="lldP"
                     /locus_tag="OO172_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5511752.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6572..7348
                     /gene="lldR"
                     /locus_tag="OO172_001522"
     CDS             6572..7348
                     /gene="lldR"
                     /locus_tag="OO172_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5511753.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7345..8535
                     /gene="lldD"
                     /locus_tag="OO172_001523"
     CDS             7345..8535
                     /gene="lldD"
                     /locus_tag="OO172_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5511754.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8731..9204
                     /gene="trmL"
                     /locus_tag="OO172_001524"
     CDS             8731..9204
                     /gene="trmL"
                     /locus_tag="OO172_001524"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5511755.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9257..10078)
                     /gene="cysE"
                     /locus_tag="OO172_001525"
     CDS             complement(9257..10078)
                     /gene="cysE"
                     /locus_tag="OO172_001525"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5511756.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10158..11177)
                     /gene="gpsA"
                     /locus_tag="OO172_001526"
     CDS             complement(10158..11177)
                     /gene="gpsA"
                     /locus_tag="OO172_001526"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5511757.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11177..11644)
                     /gene="secB"
                     /locus_tag="OO172_001527"
     CDS             complement(11177..11644)
                     /gene="secB"
                     /locus_tag="OO172_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5511758.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11707..11958)
                     /gene="grxC"
                     /locus_tag="OO172_001528"
     CDS             complement(11707..11958)
                     /gene="grxC"
                     /locus_tag="OO172_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5511759.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12100..12531)
                     /locus_tag="OO172_001529"
     CDS             complement(12100..12531)
                     /locus_tag="OO172_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5511760.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12776..14320
                     /gene="gpmM"
                     /locus_tag="OO172_001530"
                     /gene_synonym="pgmI"
     CDS             12776..14320
                     /gene="gpmM"
                     /locus_tag="OO172_001530"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5511761.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14330..15613
                     /gene="envC"
                     /locus_tag="OO172_001531"
     CDS             14330..15613
                     /gene="envC"
                     /locus_tag="OO172_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5511762.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15617..16576
                     /locus_tag="OO172_001532"
     CDS             15617..16576
                     /locus_tag="OO172_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5511763.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16563..17597)
                     /gene="waaH"
                     /locus_tag="OO172_001533"
     CDS             complement(16563..17597)
                     /gene="waaH"
                     /locus_tag="OO172_001533"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR5511764.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17836..18861)
                     /gene="tdh"
                     /locus_tag="OO172_001534"
     CDS             complement(17836..18861)
                     /gene="tdh"
                     /locus_tag="OO172_001534"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5511765.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18871..20067)
                     /gene="kbl"
                     /locus_tag="OO172_001535"
     CDS             complement(18871..20067)
                     /gene="kbl"
                     /locus_tag="OO172_001535"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5511766.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20342..21199)
                     /gene="yibB"
                     /locus_tag="OO172_001536"
     CDS             complement(20342..21199)
                     /gene="yibB"
                     /locus_tag="OO172_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5511767.1"
                     /translation="MKSSTTIITAYFDIGRGDWTTNKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKSYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21503..22435
                     /gene="rfaD"
                     /locus_tag="OO172_001537"
     CDS             21503..22435
                     /gene="rfaD"
                     /locus_tag="OO172_001537"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5511768.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22445..23491
                     /gene="rfaF"
                     /locus_tag="OO172_001538"
     CDS             22445..23491
                     /gene="rfaF"
                     /locus_tag="OO172_001538"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5511769.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23495..24487
                     /gene="rfaC"
                     /locus_tag="OO172_001539"
     CDS             23495..24487
                     /gene="rfaC"
                     /locus_tag="OO172_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5511770.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24484..25692
                     /gene="rfaL"
                     /locus_tag="OO172_001540"
     CDS             24484..25692
                     /gene="rfaL"
                     /locus_tag="OO172_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5511771.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25728..26870)
                     /locus_tag="OO172_001541"
     CDS             complement(25728..26870)
                     /locus_tag="OO172_001541"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5511772.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26879..27892)
                     /locus_tag="OO172_001542"
     CDS             complement(26879..27892)
                     /locus_tag="OO172_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5511773.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27917..28624)
                     /gene="rfaY"
                     /locus_tag="OO172_001543"
     CDS             complement(27917..28624)
                     /gene="rfaY"
                     /locus_tag="OO172_001543"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5511774.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28650..29657)
                     /gene="waaO"
                     /locus_tag="OO172_001544"
                     /gene_synonym="rfaI"
     CDS             complement(28650..29657)
                     /gene="waaO"
                     /locus_tag="OO172_001544"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5511775.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29700..30497)
                     /gene="rfaP"
                     /locus_tag="OO172_001545"
     CDS             complement(29700..30497)
                     /gene="rfaP"
                     /locus_tag="OO172_001545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5511776.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30490..31614)
                     /locus_tag="OO172_001546"
     CDS             complement(30490..31614)
                     /locus_tag="OO172_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5511777.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31611..32669)
                     /gene="rfaQ"
                     /locus_tag="OO172_001547"
     CDS             complement(31611..32669)
                     /gene="rfaQ"
                     /locus_tag="OO172_001547"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5511778.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8733 a         8070 c         7788 g         8416 t
ORIGIN      
        1 atagcgagca tcattaatga acaaaatatt taaagttatc tggaaccctg cgacagggaa
       61 ttatactgtt accagcgaaa cggcaaaaag ccgtggcaag aaatctgggc gcagtaagct
      121 gttaatttct gcgctggttg cgggtggaat gttgtcgtcg tttggggcat tggcgaatgc
      181 cgggaatgac aacggtcagg gtgttgatta cggtagtgga tcagctggcg acgtctgggt
      241 tgctataggc aaaggggcga aagcaaatac ttttatgaac accagtggtt ccagtactgc
      301 tgtgggttat gacgctatag ctgaaggcca atatagctct gccatcgggt caaaaaccca
      361 tgcgattggt ggtgcatcaa tggcctttgg ggttagtgca atatcagaag gcgatagaag
      421 tatagcactg ggtgcctctt cgtattcatt gggccaatac tcaatggccc tggggcgtag
      481 cagtgtagct agcggtactg acagcctcgc atttggcaga caatcacttg ccagcgcagc
      541 gaacgctatt gcgataggtg ctgagaccga agccgctgaa aatgcaactg ctattggcaa
      601 taatgcgaag gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa
      661 agtcaatact atcgcattag gaaatggcag ccaggctctg gcagataatg caatcgccat
      721 aggccagggc aacaaagctg atggcgtgga tgccatcgct ctgggtaatg gtagccagtc
      781 gagaggctta aacaccattg ccttaggcac agccagtaat gcaactggtg ataagagtct
      841 tgcgcttggt agtaatagca gtgccaacgg tattaactct gtcgcgctgg gcgcagattc
      901 cattgcggat ttagacaata ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt
      961 taatgtgaaa aatggcgcga tcaagtctga cagttacgat gccattaatg gttcacagct
     1021 ttatgccatt agcgactcgg tagcaaaaag gcttggagga ggggctgcag tagatgttga
     1081 tgacggtact gttacagcac caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg
     1141 ggctgcgctc gctgtacttg atgaaaacac cctgcaatgg gaccaaacca aaggcaaata
     1201 cagcgctgct catggtacta gtagcccaac tgccagcgta atcaccgatg ttgcggatgg
     1261 cacgatttca gcctccagta aggatgcggt taacggttcc caactgaaag ctaccaatga
     1321 cgatgtcgaa gccaacacca ccaatatcac caacctgacg gattccgttg gtgaccttca
     1381 ggctgatgcc ctgctctgga acgaaactaa aaaggcattc agtgcagctc acggccagga
     1441 taccaccagc aaaatcacca acgttaaaga tgccgacctg acggctgaca gcactgatgc
     1501 tgttaacggc tctcagctga aaaccaccaa cgatgctgtg gcgacgaata ccaccaatat
     1561 cgccaataac acttccaata ttgccactaa caccaccaac atctctaacc tgactgagac
     1621 ggtgactaat cttggtgagg atgcgctgaa atgggataag gacaatggtg tattcacggc
     1681 agctcatggc accgagacca ccagcaaaat aaccaacgtt aaagatggcg acctgacgac
     1741 tggcagcacc gatgccgtta acggctctca gctgaaaacc accaacgatg ccgtggcgac
     1801 gaataccacc aatatcgcca ctaacaccac caacatctct aatctgactg agacggtgac
     1861 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtcttca ctgcagctca
     1921 tggcaacaat accgccagca aaatcaccaa tatcctggac ggcacagtca ctgcaaccag
     1981 ttccgatgcc attaacggta gccagcttta tgacttaagc agcaatatcg ccacctactt
     2041 cggcggcaat gcttctgtga atactgacgg cgtgtttacc ggtccaacct acaaaatcgg
     2101 tgaaacaaat tattataacg tcggcgatgc tctgctttgg gatgccaccg caggtaaatt
     2161 cagtgccaaa cacggtacta atggtgacgc aagcgtgatc actgatgtcg cagatggtga
     2221 aatttcagac tccagttctg acgcagtaaa cggctcacaa ctccacggcg tgagcagtta
     2281 tgttgttgat gcgctggggg gtggtgccga agtcaatgca gacggcacca tcactgcgcc
     2341 gacgtacacc attgctaatg ctgattacga taatgtcggt gatgccctga atgctatcga
     2401 taccactcct gacgacgctc tgctctggga tgcggacgcc ggtgaaaatg gtgcatttag
     2461 cgccgctcac ggaaaagata aaactgccag tgtaatcact aacgtcgcta acggtgcaat
     2521 ctctgctgcc agcagcgacg cgattaacgg ctcacaactc tataccacca ataagtacat
     2581 cgctgatgcg ctgggtggtg acgcagaagt caacgctgac ggcaccatca ccgcaccgac
     2641 ttacaccatt gcgaacgccg agtacaacaa cgtcggtgac gccctggatg cgcttgatga
     2701 taacgccctg ctgtgggata agactgccaa tggcggtgct ggagcctaca atgccagcca
     2761 tgacggtaaa gccagcatca tcactaatgt cgctaatggc agtattagtg aggacagtac
     2821 cgatgcagtg aacggttctc agttgaatgc gacgaatatg atgattgagc agaacaccca
     2881 aattatcaat cagctcgctg gtaacaccga cgcaacctat atcgaagaaa atggcgctgg
     2941 tatcaactac gttcgtacta acgacaacga tttagccttt aacgatgcaa gcgcctctgg
     3001 tgtcggcgct acagctgtag gttataacgc tgtcgcgtct ggtgccagca gcgtagccat
     3061 tggtcagaac agcagcagca ccgttgatac cggtattgcg ctgggtagca gctccgtttc
     3121 cagccgtgtg attgccaaag gttctcgtga cactagcgta acggaaaatg gcttggtgat
     3181 tggttatgac accactgacg gcgaactgct aggtgcattg tcaattggtg atgacggtaa
     3241 ataccgccaa atcatcaacg tagctgatgg ttcagaagcc catgacgccg ttacggttcg
     3301 ccagttgcag aacgctattg gagcggtcgc cactacgcca accaagtact ttcacgccaa
     3361 ctcaacggca gaagactcac tggccgttgg tgaagactcg ctggcaatgg gtgcgaaaac
     3421 tgtcgttaat ggtaatgcag gtattggtat cggtttgaac actctggttc tggctgatgc
     3481 gatcaacggc attgctatcg gcagtaacgc acgcgcaaac catgcaaaca gtatcgcaat
     3541 gggtaacggt tctcagacca cccgtggtgc acagactggc tacaccgcct acaacatgga
     3601 cgcaccgcag aactctgtag gtgagttttc tgtcggtagt gaagacggtc aacgtcagat
     3661 cacaaacgtc gcagctggtt cagcggatac cgatgcggtt aacgtgggtc agttgaaagt
     3721 cactgatgag cgcgtagcgc aaaataccca gagcattact aacctgaaca atcaggtcac
     3781 taatctggat actcgcgtta ctaatatcga aaacggtatt ggcgacattg tcaccaccgg
     3841 tagcaccaag tacttcaaga ccaacaccga tggcgtagat gccaacgccc agggtaaaga
     3901 tagcgttgct attggttctg gttccattgc tgccgctgac aacagcgtcg cactgggtac
     3961 cggttccgtt gcagaggaag aaaatacaat ctctgtaggt tcttctacta accaacgccg
     4021 tatcaccaac gtagctgcca gtgttaatgc caccgatgcg gttaacgtgt cgcagctgaa
     4081 atcttctgaa gcaggcggag tgcgctacga caccaaagct gatggttcta tcgactatag
     4141 caatatcacc ctcggtggtg gcaacggcag tacgactcgt atcagcaacg tctccgctgg
     4201 cgtcaacaac aacgacgcgg tgaactacgc gcagttgaag caaagcgcgc aagaaacgaa
     4261 gcaatacacc gatcagcgga tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa
     4321 gttgagcggt ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttatacacc
     4381 gggtgccagc atggcttcta ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt
     4441 aggtgtatcg atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa
     4501 tagccagggt gaatactccg ccgcactcgg tgccggtatt cagtggtaat gatccattaa
     4561 gttagtgtga ctaagggcaa acgccaaatt gcctgatgcg ttacgcgcat caggcaagat
     4621 aaggcgctcc gggtcaacaa tctaagggca attctctgat gaggattgcc cttttcttta
     4681 ccagacatct ccccccacaa gaattggccc taccaattct tcgcttatct gacctctggt
     4741 tcacaattcc ccaattaaaa ctcacatcaa tattgataat acataacatt tagttaacca
     4801 ttcattgtca ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac
     4861 aaggagatga gcaacagact cattacacga tgtgcgtgga ctctaggaga cctgcaatga
     4921 atctctggca acaaaactac gatcccgccg ggaatatctg gctttccagt ctgatagcat
     4981 cgcttcccat cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg
     5041 ccgcctcatg gacggtagca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg
     5101 tcgctaacgc gctggcctcg gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt
     5161 ggatcattat tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca
     5221 tcattcgctc gtctattctt tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg
     5281 gtttctgttt cggcgctttc cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta
     5341 ccgccgcatt gctggtcggc ctgggtttta aaccgctgta cgccgccgga ctgtgcctga
     5401 ttgttaacac cgcgccagtg gcatttggcg cgatgggcat tccaatcctg gttgccggac
     5461 aagtaacagg catcgacagc tttgagattg gccagatggt ggggcgtcag ctaccgttta
     5521 tgaccattat cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaaag
     5581 agacgtggcc tgcggtcgtg gttgcaggcg gctcgtttgc catcgctcag taccttagct
     5641 ctaacttcat tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc
     5701 tgacgctgtt cctcaaacgc tggcaaccag ttcgcgtatt ccgcttcggt gacttggggg
     5761 cgtcacaggt tgatataacg ctggcccaca ccggttacac cgcgggtcag gtgttacgtg
     5821 cctggacacc gttcctgttc ctgaccgcta ccgtaacact gtggagcatc ccgccgttta
     5881 aagccctgtt cgcatcgggt ggtgcgctgt atgagtgggt gatcaacatt ccggtgccgt
     5941 acctcaataa actggttgcc cgtatgccac cagtggtcag cgaggctaca gcctatgccg
     6001 ccgtgtttaa gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc
     6061 tctcgattgt ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc
     6121 tgaaagaact agctctaccc atctactcca tcggcatggt gctggcgttc gcctttatct
     6181 cgaactattc cggactgtca tcaacactgg cactggcact ggcgcacacc ggtcatgcat
     6241 tcaccttctt ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct
     6301 catctaacgc cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg
     6361 atctgttgct ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc
     6421 aatctatcgc tatcgcctgt gcggcggtag gcctggtggg caaagagtct gatttgttcc
     6481 gctttactgt caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc
     6541 aggcttatgt cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga
     6601 cgaggttgcc gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa
     6661 gttgcccgct gagcgccaac tggcgatgca actcggcgta tcacgtaatt cactacgcga
     6721 ggcgctggca aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt
     6781 tattcgctgg cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct
     6841 gatggccgat gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc
     6901 cagcaccgca tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct
     6961 ttgctttgaa gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt
     7021 tcatctggcg attgccgaag cctcacataa catcgtgctg ctgcaaacca tgcgcggttt
     7081 cttcgatgtc ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc
     7141 ggttttttca caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga
     7201 tgccgacggg gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa
     7261 acgattcgat gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcataa
     7321 tgagcattcg agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca
     7381 gcgcaacgca ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa
     7441 tacacgctgc gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg
     7501 aaaaacatgt ccgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg
     7561 gtagcactgg caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca
     7621 gccaaagcgg cggacgcgca tggcattccg tttactctct cgacggtttc cgtttgcccg
     7681 attgaagaag tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc
     7741 gatcgcggct ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg
     7801 gttttcaccg tggatatgcc gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg
     7861 agcggcccaa atgcggcaat gcgccgctac ttgcaggcgg tgacgcatcc gcaatgggcg
     7921 tgggatgtgg gcctgaacgg tcgtccacat gatttaggta atatctcagc ttatctcggc
     7981 aaaccgaccg gactggaaga ttacatcggc tggctgggga ataacttcga tccgtccatc
     8041 tcatggaaag accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg
     8101 atcctcgatc cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg aattgtggtt
     8161 tctaaccacg gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct
     8221 attgcagatg cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg
     8281 cttgatgtcg tgcgtatgat tgcgctcggt gccgacaccg tactgctggg tcgtgctttc
     8341 ctgtatgcgc tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgaa
     8401 aaagagatga aagtggcgat gacgctgact ggcgcgaaat cgatcagtga aattacgcaa
     8461 gattcgctgg tgcaggggct gggtaaagag ttgcctacgg cactggctcc gatggcgaaa
     8521 gggaatgcgg catagtcgtt tgcgccccct caccctaacc ctctccctca gggagagggg
     8581 accgttcggc gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc
     8641 ctcgcccctc cggggagggc cggggtgagg ggaaaaggcc gcacggttca ccaattttct
     8701 gctatcctgc ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa
     8761 attccgccaa atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctacat
     8821 atcatcgaac cgatgggatt tgcctgggac gacaagcgcc tgcgccgcgc ggggctggac
     8881 tatcacgagt ttaccgccgt tacgcgtcat catgactatc gcgcgttcct cgaagcagaa
     8941 aatccccagc gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaagc
     9001 tatcaggatg gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt
     9061 cttgatgccc tgcccgctga acaaaaaatt cgcattccga tggtgccgga cagccgcagc
     9121 atgaatctgt ccaatgcggt gtcggtagtg gtgtatgaag cctggcggca gctgggatat
     9181 ccgggagcgt tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg
     9241 gcacgatcac aggacattag atcccatccc catactcaaa tgtatggtta ataccgttga
     9301 aatgctggtc catatccatt gatggcttat cgctgtctgg tttaccgaca atacgagccg
     9361 gaacgccagc ggcggttgta tgcggcggca ccggttgcag caccacggaa cctgcgccaa
     9421 tcttcgcgcc gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca
     9481 caccttcacg aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca
     9541 cagattgcag aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg
     9601 tggcgtggtc gagcataatg ccacggccaa tttttgctgc cgggtgaata tcgacctgga
     9661 acgtcacaga aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca
     9721 accagtgacc gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg
     9781 ttgagtattt atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag
     9841 aggcgatcat ttccgggtca gcggcgtagg cttcttcaac cacttcacgg atagcaatag
     9901 caggcataat tggcgatgac agcttgttcg ccagcatgta gctcagtgca ctgccaaggt
     9961 tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca
    10021 gcgttctggc ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg
    10081 cttactccac acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta
    10141 acaaaggttc cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg acctaataat
    10201 gtcaatgctg cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt
    10261 attggcattt caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag
    10321 ccttccacca cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc
    10381 atcatgccaa aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc
    10441 agccccgcca tgcccataaa ggtggcagga tcggcaccca gcgccgcacc aagacgcgac
    10501 atttcagcca gcccacgggt gatcagcgcc gtacgcgcat tcgcaccaaa accgataccg
    10561 tcagacatcc ccgcgccaat ggcaatgacg tttttcaccg cgccgccaag ctgcacgcca
    10621 atgaaatccg ggttgctgta aacgcggaaa cttttgccgc agtgcagcag ctgctggaga
    10681 tcatcggcaa aggtctgatc ggtcgaggcc agcgaaatag ctgtcggtaa acctgccgcc
    10741 agttctttcg caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc
    10801 tcacgcgcca cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt ggtcgcccac
    10861 accagacgcg catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca
    10921 tggctgggta cgacgacgag aatattacga ctggctgcca gcgcagtggc gagatcgctt
    10981 tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg
    11041 cgttcaagcg ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt
    11101 cttgccaggg taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa
    11161 gcattacgtt ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct
    11221 gcaaatagtt catgaacagc gcatcgaagt taaccggcgc aaggttcagt tgcgggaatg
    11281 taccgcggga taccatgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc
    11341 agtatgcgcc caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac
    11401 cgccctgctg aacttcacac aggaacgcgg tttcttcgcc caaggaggcc gttacggtaa
    11461 cacgcagtac cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa
    11521 gtttaacttc tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag
    11581 agatatcctt ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg
    11641 acatgtgtag aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt
    11701 cacacgttat ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc
    11761 acagccgcca atgtgctgtg cgtcaataaa aatctgggga accgtggtgc gaccgctgcg
    11821 tttgatcatc tcttcacgct tggcggcatt gccatcgatc ggcaactcct ggaaactcac
    11881 gcccttgctg ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata
    11941 gatttcaaca ttggccataa tttagctcct gtttttttac cctgaaattt catgttgcag
    12001 ggcggctgca aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat
    12061 tgcagctaac ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg
    12121 caagttttcg ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc
    12181 agctttcgtc agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat
    12241 aaccgatttg tctttgtgct tctcaagctc accaacattg ttggctttga tttcgctcgg
    12301 caacagattg atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc
    12361 cacaaccaca gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt
    12421 cactttcgag gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca
    12481 ggcgatactc agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa
    12541 caactcccaa cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa
    12601 atacagccag cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa
    12661 atctggcatc ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg
    12721 attcagctgt agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc
    12781 ggtttctaaa aaacctatgg tactggtgat tctggatggc tatggctatc gtgaagaaca
    12841 gcaggataac gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg
    12901 tccgcatacc ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg
    12961 taactccgaa gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac
    13021 tcgtctggac gttgaaatca aagatcgcgc tttctttgct aatccagtgc tggctggcgc
    13081 ggtacataaa gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgcagg
    13141 cggcgtacac agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg
    13201 cgcagaaaaa atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc
    13261 tgaatcctcg ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc
    13321 gtccatcatt ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa
    13381 agcctatgac ctgctgactc tggcgcaggg tgagttccag gccgatactg ccgttgctgg
    13441 tttgcaggca gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg
    13501 tgcagaaggt cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt
    13561 ccgtgctgac cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt
    13621 cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat
    13681 caaaactgcg gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc
    13741 gaaaaacgac aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt
    13801 cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc
    13861 accgaaagtg gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa
    13921 actggttgcg gccatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg
    13981 cgacatggta ggtcacaccg gggtgatgga agcggcggtt aaagcggttg aagcactgga
    14041 tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac
    14101 cgctgaccac ggtaacgctg aacagatgcg cgatccagca acgggtcagg cacacacggc
    14161 acacaccaac ctgccagttc cgctgattta cgttggtgat aagaacgtga aagcggttgc
    14221 aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc
    14281 gcaagagatg actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa
    14341 ggcgattaat accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat
    14401 ctacgccagc gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga
    14461 gcgtgaccaa ctcaaatcta ttcaggccga tatcgccgca aaagagcacg cggtacgcca
    14521 aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc
    14581 tgaagccacc cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat
    14641 tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag
    14701 cctcgccgca caactggatg ccgcattccg tcagggcgag cataccggta ttcagctaat
    14761 tctcagcggt gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa
    14821 ccaggcgcga caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcaccatgca
    14881 gcgtgctgaa ctggaagaga aacaaagcga gcaacaaacg ctgttatatg aacagcgcgc
    14941 ccaacaggcg aaactgactc aggttctgaa cgagcgtaaa aagacgctgg cggggctgga
    15001 atcttccatc cagcaaggtc agcagcagct aagtgaacta cgtgccaacg aatcccgtct
    15061 gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gagaagcgcg
    15121 tgaggcccag gcggttcgcg agcgccagaa agaagcgacg cgcaaaggca ccacctacaa
    15181 accgaccgaa agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg cgccgcgtgg
    15241 tcaggcattc tggccggttc gcgggccgac gctgcatcgc tatggcgagc agctacaggg
    15301 tgaattacgc tggaaaggga tggttatcgg tgcctctgaa ggtactgaag ttaaagcaat
    15361 tgccgacggt cgagtgattc tggctgactg gctgcaaggt tacggtctgg tggtggtggt
    15421 tgagcacggt aaaggcgaca tgagtcttta cggctataat cagagcgcac tggtgagcgt
    15481 tggttcgcag gttcgcgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg
    15541 tcggccttca ctctatttcg aaattcgccg ccagggtcag gcggtcaatc cacagccgtg
    15601 gttgggaaga taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt
    15661 ggcgctctcc tcccccgtac ttgctggcaa acttgccatc gtcattgatg attttgggta
    15721 tcgcccgcac aacgaaaacc aggtgctggc gatgccttcc gctatctccg tcgctgtatt
    15781 acccgattca ccgcacgcca gagagatggc gaccaaagcg cataacagcg gtcacgaagt
    15841 gttgattcat ctcccgatgg caccgttgag taaacagccg ctggagaaaa atacgctacg
    15901 cccggagatg agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc
    15961 ctatgccgtg gggatcaaca accacatggg cagcaagatg acctctaacc tgtttggtat
    16021 gcagaaagtg atgcaggcgc tggagcgtta caatctttac ttcctcgaca gcgtaaccat
    16081 cggtaatacc caggcgatgc gcgccgcgca aggtactggc gtgaaggtga tcaaacggaa
    16141 ggtgttcctc gacgattcgc agaacgaagc ggacatccgt gtgcaattta atcgcgcaat
    16201 tgatctggcg cgtcgcaacg gttcaaccat tgccattggg catcctcacc cttcaacggt
    16261 acgcgtgcta caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag
    16321 cagcttgctg aatgaaccgc aggttgatac ttctacaccg ccgaaaaacg ctgtgcctga
    16381 tgcaccgcgt aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc
    16441 tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat
    16501 cgtttacttc cagcatcagt ggcaaggctg gggcaaacag cacgaagcgg cgaagtttaa
    16561 cgctagcgca aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc
    16621 cattgccata agcggaaaga ccacagtaac gcctggtaac cgaccttcac gctgcgcaca
    16681 ttggtaatca ggcgcttata cataccggaa gtaaatatct cggcaatcat ccgttggcgg
    16741 gtaagaatat ccggctcttt gcgcaccgca tggcaaacac gcaatgcttc gtaagttatt
    16801 tgctgatgaa attccggata aatcgtaatt ttgtcggcat aatttcgatt taatttctcc
    16861 agcaggcggg taatcttaat atagtgacgt tgataattaa ggtttttatt cccttgtcta
    16921 tgcaaccgac tcactgacgt attatgcaga taatatttat ataatgattg ctcggtatat
    16981 cgcgcacgca gcgcgttaaa catgaattct gttgtccaga caatatcctg atgatgtaat
    17041 ccggcaataa atttaatgtt atttttaaca ataacatcac ggcgataaac ccccatccag
    17101 acaacgtgag tccaacggcg cgaagaaagc cccatccgca gccagtccgg gccggttaat
    17161 acaccggttg agcgaaggcg atcgctgggg atggattgcc aggtttctcc cgtttcacga
    17221 aaagaccagt cagcgttgca ctgcgccacg tcgaggtcgt cctctaacgc catggtcatc
    17281 agcgtttcgt acatggtggg atagacctca tcgtcagcat cgacaaaagc gacatatttg
    17341 cccgtcgcca cctcgatccc acgattacgc gctaccgatg cgccagcatt cgcctgatgc
    17401 aacaaacgaa cgtgcggata gttttctgcg taatgctttg ctatttcaac agaattatcc
    17461 gttgaaccat cgttaataat aatgatttcc agagcagtcc aggtttgcgt aattaaagat
    17521 tccatacaag tgcggaaatc atcgcccgca ttatataacg gaataataac actaagttta
    17581 ttggtgctgt tcatcataaa ttaccgacga cctctgaagt tatcctgacg ttttacaggc
    17641 tttgtattaa gaaactatta agcctgtgag atattatttt tacaggtgag agagtcactt
    17701 cagggtagag agataatgag aacagctata taaaagatca gcgcaattac gaactcttta
    17761 ttacactgac aggattttac ataattaaca gattatcctc agataaaagg gctggaatac
    17821 cagcccttgt tcgtgttaat cccagctcag aataactttc ccggactggc ccgaacgcat
    17881 agcgtcaaag cccttctgga aatcatcgat agagaaacga tgggtaatga tcggcgagag
    17941 atcgaggcca gactgaatca gcgccgccat cttgtaccaa gtttcaaaca tctcacgacc
    18001 gtaaatgcct ttaatgaaca agcctttaaa gatcactttg gtccagtcga tagacatatc
    18061 agacggtgga atacccagca tcgcaatacg accgccgtgg ttcatggtat cgagcatggt
    18121 acgaaacgct ggcggcgcgc cggacatttc cagaccgaca tcaaagcctt cggtcatgcc
    18181 cagttcagcc atcacatcat taaggttttc tttcgcgacg ttaaccgcac gggtgatacc
    18241 cattttgcgc gccagctcaa ggcggtattc gttaacatcg gtgatcacca cattgcgtgc
    18301 accaacgtgt ttcgccaccg ccgcagccat aatacctatc ggacctgcac cagaaaccag
    18361 cacatcttcg cccaccagat cgaacgacag cgcggtatgc acggcgttac cgaagggatc
    18421 aaaaatggaa gccaggtcgt cggagatatt gtcggggatt ttgaaagcgt tgaacgccgg
    18481 aatcaccaga tattcggcga agcagcccgg acggttaaca ccgacaccaa tggtgttgcg
    18541 gcacagatgg gtacgaccgc cacgacagtt gcgacagtga ccgcaggtga tatggccttc
    18601 gccagaaacg cgatcgccga ttttgaagcc tttcacttcc tgaccaatac ctaccacttc
    18661 accgacatat tcatggccca cgaccatcgg caccggaatg gttttttgcg accactcatc
    18721 ccagttatag atgtgaacgt cagtcccgca gatggctgtt ttacggattt taatcagcag
    18781 atcgttatgc ccgagttccg gtacagggac gtcggtcatc cagatgccct cttccgcttt
    18841 cagtttggat aacgctttca tctcacatcc tcaggcgata acgcccagtt gtttaccaat
    18901 acgcgtgaat gcttctactg cacgcgtaat ttgctcaggg gtatgcgccg cagacatctg
    18961 ggtacgaata cgcgcctgac ctttcggaac gaccggatag aagaaaccgg tgacgtagat
    19021 gccctctttt tgcagctctc gggcaaattt ctgcgccact accgcatcac caagcatgac
    19081 cggaataatg gcgtgatcgg ctcccgccag ggtaaagccc gccgccgaca tttgctcacg
    19141 gaactgacgc gcgttcgccc acagacggtc acgcaactcg ctacccgctt caaccatctc
    19201 cagcactttg atggaagcgg cgacaatcgc tggtgccagc gagttggaga acaggtacgg
    19261 acgagaacgc tggcgcagcc actcaaccac ttctttgcgc gccgcggtat aaccaccaga
    19321 agccccgccc agcgctttac cgagcgtgcc ggtgatgatg tcgacacgcc ccatcacatc
    19381 gcagtattca tgggaaccac gaccattttc accgacaaaa ccgaccgcgt gggagtcgtc
    19441 taccatcacc agggcatcgt atttatctgc cagatcgcaa acgcccttca gattggcaat
    19501 cacgccgtcc atcgagaaca caccatcggt ggcaatcagc acatgacgcg caccggcttc
    19561 acgcgcttct ttcagacgag cttcaagctc ctgcatatcg ttgttggcat agcgatagcg
    19621 tttagcttta cacagacgca caccatcaat aatagacgcg tggttcagtg cgtcggagat
    19681 aatggcgtct tccgcgccca gcagcgtttc aaacaggcca ccgttagcat caaagcagga
    19741 agagtagaga atcgcatctt ccatccccag gaatgccgcc agtttttgtt caagctcttt
    19801 atggctgtcc tgagtgccgc aaataaaacg caccgaagcc atgccgaaac cgtgagaatc
    19861 cattcccgcc tttgctgccg caatcagatc cggatgattc gccagcccga gatagttgtt
    19921 ggcacaaaag ttaatgacgt ggcttccatc agccacggtg atatctgctt gctgcgcaga
    19981 cgtaataatg cgctcttctt taaacaaccc ttccgcccgt gcggtttcca gatcgttggt
    20041 taactgctga taaaattctc cacgcattgc gattctccag acagggcaaa ttccagcaca
    20101 tattacccaa acttataggt cgcgacgaga tgatgcgtta acacttctgc aaaattcagg
    20161 ataaataacg tgtatcttga tgtctctcaa cacctacaga tagtagaaaa actgtacagt
    20221 tttggctgag cataatgatg agtgatttta ctgcattgat ataaaaagaa aaaatgatgc
    20281 caaagcatca tttttcctat ctcagttggc atcaaaacgc tacccactaa tggagggaaa
    20341 cctattttct tgaaaggaaa attctcagcg cctgcatttt tgcccctaaa gtgttactcc
    20401 tgaagcagcg aaacaaatcg aaccattttc ctctccccag atagtttagg ttgaagagat
    20461 cggaacgttt gtaatagcac atgacaaaaa tgccctggtc atcgtccaca atgttattgt
    20521 ttagtgttat tttttgactt tccagaacta atttatagaa ttctttccat ttatgctgtg
    20581 aaccaacaat tgcgccccca ataatatata catgattgcc aatcatgaaa tcaaaagcct
    20641 gttgttgaga tgtaacagta agcccttttt ttatagtgaa aagatgcatc ttattttcat
    20701 caaatgggaa atcccaaatt ttcaatcctc gagttacatt tggcttacgg caatagccaa
    20761 aatctatcca cgcaacaaga ggcgttttca ctaaacccat gttgatagct ttatttacaa
    20821 aataggactt aaggttacat accaaaacat actctggtga ccagtactct gggtttttta
    20881 attgtcgagg ctcaagtcta tttgtaaacg actcatcttt ttgaattttt tcgatacgac
    20941 ttctgatata tctaaatttc ttttttatat cgataacgat aaccgttgtt ggttttccgt
    21001 tgcgaatcgc ttcaatcctt ggtttcaaat cgggagaagt gaaaataatc atctcatttt
    21061 caagtgcggc cagacgctca aagtaactaa aataaacatc aactgaacga gctagttttt
    21121 cacggaaccc tttattagta gtccaatccc ctctaccaat gtcaaaatat gcagtgataa
    21181 ttgtggtgga tgatttcatt actaataaga ccattaatat aaaaaaagaa ttatatcata
    21241 aatattaaca gaatgtatac tgtatattat ccattcaaat gattccgtta tttatcatac
    21301 atcggaatat tgatactaaa gcactattca catgcaaaac caacatccgc catgaaggac
    21361 tagctaaaac ccaaactagt ttgttgcaat tagcatcctt gcacctctat gtaaagggct
    21421 gaagggattc ggatgtgatg gtatgattac agacattcgt gtctgagatt gtctctgact
    21481 ccataattcg aaggttacag ttatgatcat cgttaccggc ggcgcgggct ttatcggcag
    21541 caacatcgtt aaggccctga atgataaagg catcaccgat attctggtgg tggacaacct
    21601 gaaagacggc accaagtttg tgaacctggt ggatctggat atcgcggact atatggataa
    21661 ggaagacttc ctgatccaga ttatggctgg cgaagagttc ggtgatgtcg aagcgatttt
    21721 ccacgaaggc gcatgctctt ccaccaccga gtgggatggc aagtatatga tggataacaa
    21781 ctatcaatac tccaaagagc tgctgcacta ctgtctggag cgcgaaatcc cgttcctgta
    21841 tgcctcttcc gcagccacct acggcggacg cacctccgac tttattgaat cccgcgagta
    21901 cgaaaaaccg ttgaatgtct acggttactc aaaattcctg tttgatgaat atgttcgtca
    21961 aatcctgcca gaagcgaact cgcagattgt tggcttccgc tatttcaacg tttatggacc
    22021 gcgtgaaggc cataaaggca gcatggcgag cgtcgctttc catctcaaca cccagcttaa
    22081 taacggtgaa tcaccgaagc tgttcgaagg tagcgagaac ttcaaacgtg acttcgtcta
    22141 tgtgggcgac gtggcagatg taaacctgtg gttcctggaa aatggcgttt ccggtatctt
    22201 caacctcggc accggtcgtg cagaatcctt ccaggcagta gcagatgcta cgctggctta
    22261 tcacaagaaa ggccagatcg aatacattcc gttcccggat aagctgaaag gccgctacca
    22321 ggcgttcact caggcagatc tgacaaatct gcgcgcggcg ggttacgaca aaccgttcaa
    22381 aaccgttgct gaaggtgtaa cggaatacat ggcctggctg aatcgcgacg cataagagct
    22441 ctgcatgaaa atactggtga tcggcccgtc ttgggttggc gacatgatga tgtcgcaaag
    22501 tctctatcgc acgctccagg cgcgctatcc ccaggcgata atcgatgtga tggcaccggc
    22561 atggtgccgt ccattattat cgcggatgcc ggaagttaac gaagctatcc ctatgcctct
    22621 cggtcacgga gcgctggaaa tcggcgaacg ccgcaaactg ggtcatagcc tgcgtgaaaa
    22681 gcgctacgac cgcgcctacg tcttaccaaa ctccttcaaa tctgcattag tgcctttctt
    22741 cgcgggtatt cctcatcgca ccggctggcg cggcgagatg cgctacggtt tactcaacga
    22801 tgtacgcgtg ctcgataaag aagcctggcc gctaatggtg gaacgctatg tcgcgctggc
    22861 ctatgacaaa ggcattatgc gtaccgcaca agatctgccg cagccattgt tatggccgca
    22921 gttgcaggtg agcgaaggtg aaaaatcata tacctgtaat caattttcgc tttcatcaga
    22981 acgtccgatg attggctttt gcccgggtgc ggagtttggt ccggcaaaac gctggccaca
    23041 ctaccactat gcggaactgg caaagcagct gattgatgaa ggttatcagg tggttctgtt
    23101 tggctctgcg aaagatcatg aagcgggcaa tgagattctt gccgctttga ataccgagca
    23161 gcaggcatgg tgtcggaacc tggcggggga aacacagctt gatcaagcgg ttatcctgat
    23221 tgcagcctgt aaagccattg tcactaacga ttctggcctg atgcacgttg cggcggcgct
    23281 caatcgtccg ctggttgccc tgtatggtcc aagtagcccg gacttcacac cgccgctatc
    23341 ccataaagcg cgcgtgatcc gtctgattac cggctatcac aaagtgcgta aaggtgacgc
    23401 tgcggagggt tatcaccaga gcttgatcga cattactccc cagcgcgtac tggaagaact
    23461 caacgcgcta ttgttacaag aggaagcctg acggatgcgg gttttgatcg ttaaaacatc
    23521 gtcgatgggc gatgttctcc atacgttgcc cgcactcact gatgcccagc aggcaatccc
    23581 agggattaag tttgactggg tggtggaaga agggttcgca cagattcctt cctggcacgc
    23641 tgccgttgag cgagttattc ctgtggcaat acgtcgctgg cgtaaagcct ggttctcggc
    23701 ccccataaaa gctgaacgca aagcgtttcg tgaagcgcta caagcagaga actatgacgc
    23761 agttatcgac gctcaggggc tggtaaaaag cgcggcactg gtgacacgtc tggcgcatgg
    23821 cgtaaagcat ggattggact ggcaaaccgc tcgcgaacct ttagccagcc tgttttacaa
    23881 ttgtaagcat catattgcaa aacagcagca cgccgtagaa cgcacccgcg aactgtttgc
    23941 caaaagtttg ggctatagca aaccgcaaac ccagggcgat tatgctatcg cacagcattt
    24001 tctgacgaac ctgcctacag atgctggcga atatgccgta tttcttcatg cgacgacccg
    24061 tgatgataaa cactggccgg aagaacactg gcgagaattg attggtttac tggctgattc
    24121 aggaatacgg attaaacttc cgtggggcgc gccgcatgag gaagaacggg cgaaacgact
    24181 ggcggaagga tttgcttatg ttgaagtatt gccgaagatg agtctggaag gcgttgcccg
    24241 cgtgctggcc ggggctaaat ttgtagtgtc ggtggatacg gggttaagcc atttaacggc
    24301 ggcactggat agacccaata tcacggttta tggaccaacc gatccgggat taattggtgg
    24361 gtatgggaag aatcaagtag agtgtcgttc tacaagtatg tctcttgcag atttgccagc
    24421 tcaaaccgtt tttcaaaatt taaatcttga aataataacc aataagttga catcggagat
    24481 aagatgacct caacattatt tttctctctc gagaaaaaaa actggatagc gtactggaac
    24541 agagctctcg tattcttatt cattaccacc tattttttgg gtgggattac aaggtataaa
    24601 catcttattg ttattcttat gacaataacg acaatcgtct atctctgcaa acggccaaaa
    24661 cactatctct cactatttaa aacatttctt tttggtagtg ttgccatatt aactattgct
    24721 gcattgctgt cacttcttca atcccctgat gcaggtgcta gcatgaagga aattttcaaa
    24781 gctattattg agaatacttt actatgcaca atagcaatac cggtcatatt gagagacgag
    24841 aaaagagaag atgtcgaaaa aatcgttttc ttctcattta ttagtgcgtt gggcttacgc
    24901 tgtttttctg aattgattac ctattataag gactatcaac aagggataat gccattcgca
    24961 gattatagac accgtagcat ttctgactcg atggtctttt tattccctgc attgttaaat
    25021 ctctggctta tcaaatcagc aaaataccgc atttcttttg tggttctaag cgttattttt
    25081 atttttctga tattaggaac tttatccaga ggggcctggc tttccgtgtt agtcattgga
    25141 ttaatatgga ttctgatgtt taaacaatgg aagttactat tagtaggagt aatggtttcc
    25201 atcattgcat tgtcggttat tttcacacat aaggagatga ccgcaaagct aacgtataaa
    25261 cttcaacaaa ctaatagttc ttatcgctat gcaaatggta ctcaaggcag cgcactcgat
    25321 ctaatattag aaaatcctgt tattggttat ggttacggta acgttgcata taaagatgtc
    25381 tataataaac gtgtcattga ttatccagaa tggaccttta gacaatcaat agggccacat
    25441 aattttgcgc tattcatctg gtttggcact ggtttattag ggctggtaag tcttatgatg
    25501 ctatactgtg caatattgaa agagtgtata aaaaatggcg tcaagaataa atatcgctca
    25561 ccatataatg catattatat aatcttacta tcttttatag gttattttgt tatccgtgga
    25621 aacgtagaac aaattgaacc aaatttatta ggcgtttacg ccggcttatt attagcgatg
    25681 aaaaacaagt aaaaaataaa aaaggctgca taatgcggcc tttttattta ttcaaaccaa
    25741 ttatgaataa cctcttcgaa tctttgagtt acgccatccc aactgtagtg atcaaacaca
    25801 aagtcctgac cttgctttgc aactgctgtt aattcaggat tcgctaacgt ttttaaaata
    25861 tcgctgctta tggagtctgc agtcataggt tcttttaaat ggaatcccgt agtattttct
    25921 ttgacaaatt ccgtcatccc cccacgcgta ctcactaaaa caggcttacc agcacccata
    25981 gcttcaattg ccaccatgca aaatggttcc tggaactggg aaggaatgac gactaaatct
    26041 gccagtgggt aataacaatg catcttttca ggcggtatgc ttcctaacat aatgcaacga
    26101 tccttcaaac gttttgcaat ttccctgaca ttgcgttgat atgcaccttt gtcactttta
    26161 ctcatttctg tataatcacc aatcacaact aattttaaat ttttgtgagc agcggccaat
    26221 ttttcgaatg attgcatcaa cagcaatatg cctttgtcag ggacgatacg cccagcataa
    26281 aaaataattt tctcttcagg ggagatatta agttcgcttc tagtgatcgg ctgaaaatta
    26341 gattgatagg tttctaaatc aattccattt ggtacaatct caatatctgc atttgctaaa
    26401 taagattggt aatacttttt caagtataaa ctagggacta tcattttaac attttgctca
    26461 agcaattttg gttcaaatgc attatgcata tgtataacca ctcttgcctg aggagctcgc
    26521 ttacgaattt gagtgtatag cttcatgcta ttatgaacta caataacact ttctttcgtt
    26581 ataggaaaat catgtgcaat attcaatatt ctctgtgaat atggaagagg atcaagtctt
    26641 gtccattttt gaaataaccg cttatatatg cgactgaatc caaccctatg aatactacaa
    26701 ttatcattaa caaaggtgta atttgagtat cctggatttt tgatacaaac aatacgattt
    26761 ggaaaattgg tacgctgagc aacctgatac atccacgttt caacagcagc cgctccgcga
    26821 ggaggaattg aaaaaatagg agtaaccgta aatattattt tatcaaccat ctcgtatatt
    26881 aacctttcat aacattatat ttaattgctg taaaaacacc atcgagaaat tttttctggt
    26941 aaagcaagtg tttatatttt tctttgtgct catgtttttt aacagctttt ttataaggta
    27001 tatttctcca tggtgatata ttataaatat tacgaaaata atctgcagag gcgtagttcg
    27061 cccaatcatg ccatggctta gttataccag tataatgtat gaagacagtg tcatcattaa
    27121 taaaccgggt gtaatattca ctattttttt cttcaaattc tgactttata gtataaatac
    27181 aattatattt tctcggtaat attttagcat gatgtagaaa cattaagttt aaaatatctt
    27241 gatccgggta ctttaatttt tttataattg actcatctga taatagatca aagaattttt
    27301 ccgttagtct ttgttttaac cattcccgta aattaatata cattacacca gagttaaaat
    27361 agctaccgtt aaaatcctca ttacacaaac gctcagcgct tttactttgc atagcatcta
    27421 catcaattac cactgcccca tattcatctt taaactctaa tgctattaac tcgttcaatg
    27481 aacccttaca gacgatatca gcatcaagat ataataatga atccaatcgc gcagaaaaat
    27541 aatcaaatga aagcaaacga taataaatag atactggcca gatatttgat tgaggtagcg
    27601 ccttcaatgg ctcagaatca atatgatata atttaattac tgtttgatac ttcgttgcta
    27661 attgtgataa taattcaata tactctttgt ctaagtaatc agcgattatg tgaaaggtaa
    27721 atttgttttg tttattcttt tcaagaattg aagcaattga aatcgcagcc ccaacctggt
    27781 agttctcatc aataccccat gagacattaa acgtttcccg ttcatcaagt ttaacggggc
    27841 gcttgtccag ttcaattata tctttaaatt gagtaagatg tttaaaatcc aatttaatta
    27901 cccttcatca taatcattat ttgctcttca atcctagttt gacttttaca tccctgatta
    27961 cttttttaat tttctttttg aatataagat atgtatagcc aaagtctttc aattcatttt
    28021 ttatattata gtgccgttca agatcaatgc gatcttttgc ttttaaaaca gcggttgttt
    28081 ttttacctga aaggtcaatt agtcttaaac ctttttcgga tactataaaa tttcctttat
    28141 gaggatcgcc agacaccatt ccatgctgat gcaactcttt tatcgattct gataattgat
    28201 ccttcagatc ttcagaaatt tccaaatact catttaaccc gacaccttca atatactcaa
    28261 tgagcataat ataataatga gcgaaattta atgttttacg ttcagctagc aaaaaatagt
    28321 cattaattga ttggatgcct tcaccacgta cccgatcggt ttgataaatt aaattttcat
    28381 aataatcttt ttttatgcaa gattttaaga aacgttcggt cattttatgc tttggcgcat
    28441 aaactttaag tacaagaggt ccacgagcag tgtcaataag aatgacttta gtatcatcaa
    28501 tagaacgaaa aacttttaat gttttaaccc tgcaagttaa aaagtcattt agaacttgct
    28561 cataaaaggg atcattatct tttatgaaaa ctttcaaccc atttatcgtt ttattatata
    28621 tcataatgaa ttattttaac cttaaatctt tagaagcatt tttctttata atactttaaa
    28681 taattaaaaa tgcctgccat ataatgcttt tgtttaaatt tatgttttgc acaataccga
    28741 tactgattgc tatttacagg tttaagtaaa tcttctttac tccacggaga agctgctttg
    28801 gcgatcaaaa aactacgtga aaccggataa tcagcccact catgccaggg ttttgtaggt
    28861 cccacatagt gtataaacac ggtgtcatca ttgactggat tatcaacttt gtcttttaat
    28921 tcatagttaa tactatatcg ggtattatat ttctccgaaa taaacttcac tttaccattc
    28981 aataatacat tcagtacatc ttgatcaagg tgggtgattt tacttaccca atctgggtca
    29041 cgcaacattt caatagcttt tgacgaaatg ttatttagat tccactcatc aatatttatc
    29101 agtaaaaaac cagcattgaa atatccagaa gctaactgtg gtgtagttaa taccgaggct
    29161 cgattttgcc accattcaat atctctttca gctacaactg cggcaatttc attagtagaa
    29221 aattgataat ctaagagttc tttaatacta cccttgcaag caatatctgc atcaagatat
    29281 agtatttttt catgtttatg atagaaataa tctgcaatta taaatcgaaa atatgttgcg
    29341 taagtccagt ttttcgtgct tggtaatgac ttcagcttat cacaattgat aacataaata
    29401 ttaattcttg aattatattg ttttgctaaa tcagaaaaat ataatttgtc tttatcactg
    29461 atataatctg tgaaaacatg aaattcacaa gagatttctc tattgtttaa tagaatagat
    29521 gcgatggcta caccacatcc aaaaagaaaa tttctgtcaa taccaaatgc tatatcaaga
    29581 gtatttttag atcttatatt ttgataatga aaattatact caaaaaggat gatgtcacta
    29641 tcattgagtt gagacatatt tattctgtat ccaaaatttt ggcaaaaata agttacgggt
    29701 tacaacgatt ttcgaatcgt tctttccctg atttttgtgg cttttgcttc tgcttgcgac
    29761 agcagtcctt gttcctgctt gagaatgtct ttaagcgggg cggcaaaata caccttcata
    29821 aagcgccaga tatcccgctg agtcaggcca atattcattg aagaaaaata aagccctata
    29881 agatctttat cgcgccaacg acctggaacg cgcgtgcgaa gctgcgcccg gtgtaggtca
    29941 attaccgaaa tttttaactc ctcttcctta ccggaaaaag gcaagtgcag caggaaatga
    30001 cagatataac agtcacgatg gttaatgccc gcagcatgca tatcgcgcac catcgtcgcg
    30061 acacgcttaa taagcatacg ctttacgcga acatctggtg ggttggtcgc ccagtcagca
    30121 cagtaatctt ccagactgat ggttggtgtc agatcttcgg taataataaa cgaggttctg
    30181 gtcagcggat tcatgccttt ttcgccgaat gccaccccat acatagtatc aacgccgaca
    30241 tcccgcagtc gatgaatcgc attccattcg cggtctgcgc ctaatactgg catccgcaat
    30301 gagagtaaat ttttgattat ctctttcagg gtcgtgccgc gatgccattt gagaaaatag
    30361 ctttttcccg ccatttcaaa gcgcagagta cggcgagttt ccagttcacg aaatacctca
    30421 ccctgcaagg ttttaacttc ctcaaaagga tctttgccgc gccataacgt ggcaaacggc
    30481 tctttaagtt caaccatcca gaccacccgt tatgatatcc gccgctttct ctggcagact
    30541 gtataaatct tgtgtatcag cataatgtcg cgcattttcc gcccaagcct ggcgcaatga
    30601 agattgcgtt aacgctttgc gtaaaatctc attcaatgtt tcctggcgga atggctcagc
    30661 aatagcctcg ccgcaattag cgtcgacaat ataatgcgca tagccacaaa cggcagttgt
    30721 tagtaccggt aatcctgcag taatcgcttc cagcagcaca attcccgccg cttcctggta
    30781 ggcaggatgc agtaataaat ccgccgccgc cattaattcc gagacatcgt tgcgccccga
    30841 gaagaagtga acattactgc gcacgccgcg tttttctgcc agtgcctcaa attttcgcgg
    30901 tttatcctgc ccaacaacat atagcaatgt gttgtggcgc agcgaatccg gtaacgaagc
    30961 aagtgcttca atggaacgat cgacaccttt acgcgtgaag tctgaaccga cctgcaacaa
    31021 taaatattgt tgttcggtta ttccattctt cttacggaag atttcacggc tattggctgg
    31081 ctgctggcta tatttacgat caggataaat ccccggtggc agaatatgaa aacgctccgc
    31141 ttcagtctga taatgtttct ggaaatcggc gatttgctta tctgtcagca tcagcagctg
    31201 tgtcggcttg ccctgttcga aggttgcccg ctcaaaggcg gcataatggc gataacgtga
    31261 cgtcaggcga tagaaaaagc ctttttcctg cgctactttc tcggcataac aaacatcagc
    31321 ggcataataa acgtccagcc ccggcatttt gttgaatcca acgactttat cgacgggatg
    31381 ttcgcgtaaa tgtttttgca cccaggcaaa atactccgca ttgcgcccgt gattggtatg
    31441 cgatttaacc ggcactttga tcagttcaaa tacatcaggg cattcgcctt cccacgactg
    31501 ggtataaacc cgaacatgat gacctcgggc ggcgactgtc tgagcaatac gcataaaatc
    31561 gcgctgcaaa ccgccaaagg gaaaatattt atataaacaa aaagcaacga tcataacgac
    31621 gtacctgtcg tggaggaggg cagtaattta tcgacagcag caatgacatc tgccgccgga
    31681 ataaccgaaa gatacatctc atttcggtca cgctgatcgc gcgttggcat ttcccggtaa
    31741 tctcccgccc agaattgaat catgttattt gaccagggac gccagaaaat atgatctgtt
    31801 gcgccaaaca gcgatatcag cggcgtatta actgcagcgg caatatgcgc cggtgcggaa
    31861 tcaacgccaa taaacagctg cgcatgatcg attaacgcac caagttccgg gaaggtcacc
    31921 tttccagcca gcgccgttac tggtggcgtc tggcatccct gcgcaatttc attgacgcag
    31981 gccagatcgt ctttatctgg gccggacgtc agaacgactt cataaccacg agcatgtaag
    32041 gcatcaatca cagcggaaaa cttggcgttg tcccagcatt tgaagatttg ccgcgccgta
    32101 ggttggataa ccacataatt ttgtccaaca ccagcgtgat caagttcgcg acgcatacgt
    32161 ttccagcttg caggcgggta actcatggtt gtctgcttca ccaacgaatc aagtcccaat
    32221 ggtgtcagca cggataagtt actttccacc acatttccac cctgcaacgg caccaaatgg
    32281 gtgaaacttt tacgccaaaa agcagactgc cgatgatgat aatcctggga aattttcaca
    32341 cgagcattta ataagcgaac cagtatagca accatccatt gatcggtaag attgacgata
    32401 aggtcatact tattggcacg taataccttg atgagatgaa aaaagttggc aattttttct
    32461 gaggcttttg cttttttatt ttttatgccg tagagcgcgt taatctctgg attttcagac
    32521 aggatcggga tggtgtcctg ataaagcagc acatcgattt ttgcgtcagg gtaatttttt
    32581 ttcagagaac taatgacggg agtagttaat aacatatccc catgaaaacg catcttaatg
    32641 agcaaaattc ttcgaaatgg cttatccaca agtaactctt tttgttaaga ttatccgatt
    32701 aaggtgaagc agaaaaagca cgctaccgcc ccaggctcaa cagctacctg aatactgata
    32761 ccgcactgat attgacgcgc tagtgtatca cttctttcgc ggcaatccga cccgaatgat
    32821 ttttaagcac attgttgatt ttcaataaag aaatcaatca aataaaatca ttagcaacca
    32881 tattctttca ctgttgctct gcgattcctt cctgcacgta cagcagaaat caatgaatcg
    32941 cataaaaata caaaaaaagt atagatgttc agcaagaatt ggcgggtaaa ttgctattta
    33001 agtcaaa
//
LOCUS       DAJTNM010000030        32930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTNM010000030 DAJTNM010000000
VERSION     DAJTNM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.030"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..328)
                     /gene="yfdR"
                     /locus_tag="OO172_001548"
     CDS             complement(<1..328)
                     /gene="yfdR"
                     /locus_tag="OO172_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5511779.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDSVIR
                     EKYGLPP"
     gene            complement(456..1280)
                     /locus_tag="OO172_001549"
     CDS             complement(456..1280)
                     /locus_tag="OO172_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HCR5511780.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(1346..1726)
                     /locus_tag="OO172_001550"
     CDS             complement(1346..1726)
                     /locus_tag="OO172_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511781.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGH"
     gene            complement(1723..2033)
                     /locus_tag="OO172_001551"
                     /pseudo
     CDS             complement(1723..2033)
                     /locus_tag="OO172_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2309..2605)
                     /locus_tag="OO172_001552"
     CDS             complement(2309..2605)
                     /locus_tag="OO172_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511782.1"
                     /translation="MLKQVYEMKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIM
                     AGVVFSLRRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            complement(2778..3452)
                     /locus_tag="OO172_001553"
     CDS             complement(2778..3452)
                     /locus_tag="OO172_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HCR5511783.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            3543..3743
                     /locus_tag="OO172_001554"
     CDS             3543..3743
                     /locus_tag="OO172_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5511784.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            3787..4338
                     /locus_tag="OO172_001555"
     CDS             3787..4338
                     /locus_tag="OO172_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511785.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDEQYLSISKLQEHAALVYKIFCVSE
                     SSDARECAAPGAVACRDCGETNA"
     gene            4514..4693
                     /locus_tag="OO172_001556"
     CDS             4514..4693
                     /locus_tag="OO172_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5511786.1"
                     /translation="MRQVNRWFKEHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            4683..5624
                     /locus_tag="OO172_001557"
     CDS             4683..5624
                     /locus_tag="OO172_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5511787.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            5621..6115
                     /locus_tag="OO172_001558"
     CDS             5621..6115
                     /locus_tag="OO172_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5511788.1"
                     /translation="MSMSLLNDVQKFIEAHRGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            6115..6768
                     /locus_tag="OO172_001559"
     CDS             6115..6768
                     /locus_tag="OO172_001559"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCR5511789.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVLTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            6765..7091
                     /locus_tag="OO172_001560"
     CDS             6765..7091
                     /locus_tag="OO172_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5511790.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            7088..7477
                     /locus_tag="OO172_001561"
     CDS             7088..7477
                     /locus_tag="OO172_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5511791.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITINKYEKTAGAAA"
     gene            7497..8294
                     /locus_tag="OO172_001562"
     CDS             7497..8294
                     /locus_tag="OO172_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR5511792.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSQDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVSRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            8302..9291
                     /locus_tag="OO172_001563"
     CDS             8302..9291
                     /locus_tag="OO172_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5511793.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            9306..9674
                     /locus_tag="OO172_001564"
     CDS             9306..9674
                     /locus_tag="OO172_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCR5511794.1"
                     /translation="MMRDIQMVLERWGAWAASDSSGVDYSPIAAGFKGLLPYTSKKRL
                     ACSDSDALIIEGCLARLKQKRPYEHSLLVAHYLYRISKRKIAKARGKDEKLIRIEIQL
                     AEGFIDGCLSMLDLTLDLDV"
     gene            complement(9703..11034)
                     /locus_tag="OO172_001565"
     CDS             complement(9703..11034)
                     /locus_tag="OO172_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HCR5511795.1"
                     /translation="MLDTLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTG
                     KTEFSIKLKNLIIEQETESKVVYIDAFKGDHAESPLLLITSAIASILPEEEKQHFIKR
                     SLPAIRFGLKTVLKAGAGWFLRQEASEVSEEFQDAMKKASNAAIDGTIENLLEDHMES
                     EKNINSLKSCIESISESQKIVIIIDELDRCKPSFSTNVIETIKHIFDINNVFFILVTN
                     TEQLKASINHIYGYSINSQKYLDKFIKYTITLPDTCLINGHVVCKTSVIYWNYLVDKT
                     KSLNEINRLSGRFIRDLIQRTNLSLRETQTFSRNLNIFQLLNDDEYKSSDPLINKIFV
                     VAVFIHCFGDKEKLKQEITAESISHLADLLNIKEIPYSYERRSQIPEISIVFFGIIKD
                     SITLNERFAPKSDEELKKFTTVYTDYEHLNFWSTTPRELLIKYINQMSFIQ"
     gene            11331..11657
                     /locus_tag="OO172_001566"
     CDS             11331..11657
                     /locus_tag="OO172_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR5511796.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            11661..12137
                     /locus_tag="OO172_001567"
     CDS             11661..12137
                     /locus_tag="OO172_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5511797.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLALKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKEAGGVVNEAEL"
     gene            12121..12513
                     /locus_tag="OO172_001568"
     CDS             12121..12513
                     /locus_tag="OO172_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCR5511798.1"
                     /translation="MKLSYKLLIVGFFVTVIGSFIWSANHYYSKYQYEKKRADEAVRN
                     AESATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRAREDIKVAVADDDCASRPVP
                     DVAADRLRKYADSIRAGSNDAVTDEPAR"
     gene            12976..13308
                     /locus_tag="OO172_001569"
     CDS             12976..13308
                     /locus_tag="OO172_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511799.1"
                     /translation="MPVNIDPEKLNDEREQVIAKWLFKDVDLISQQIELGEENVKRFD
                     ELLSIFDCCQSSWFATEHLFDNTELEKVWHEFESNFNKYINGGESKDLLMKMLDKLIS
                     SRFVFESR"
     gene            13359..13709
                     /locus_tag="OO172_001570"
     CDS             13359..13709
                     /locus_tag="OO172_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5511800.1"
                     /translation="MPPRIPKACRVRGCRSTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRVRVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKEKTARERLK"
     gene            13835..14329
                     /locus_tag="OO172_001571"
     CDS             13835..14329
                     /locus_tag="OO172_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5511801.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEELPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            14563..16059
                     /locus_tag="OO172_001572"
                     /pseudo
     CDS             14563..16059
                     /locus_tag="OO172_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            16071..16253
                     /locus_tag="OO172_001573"
     CDS             16071..16253
                     /locus_tag="OO172_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511802.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            16253..17494
                     /locus_tag="OO172_001574"
     CDS             16253..17494
                     /locus_tag="OO172_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5511803.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADDIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPSCVVPKLNSSWEPVYQVTFPDGSADVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            17472..18122
                     /locus_tag="OO172_001575"
     CDS             17472..18122
                     /locus_tag="OO172_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5511804.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRTKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYIALSLRDAEDVGSALNALKNLNF"
     gene            18137..19342
                     /locus_tag="OO172_001576"
     CDS             18137..19342
                     /locus_tag="OO172_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR5511805.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            19392..19592
                     /locus_tag="OO172_001577"
     CDS             19392..19592
                     /locus_tag="OO172_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511806.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            19595..19918
                     /locus_tag="OO172_001578"
     CDS             19595..19918
                     /locus_tag="OO172_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5511807.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            19915..20325
                     /locus_tag="OO172_001579"
     CDS             19915..20325
                     /locus_tag="OO172_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5511808.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRLRVDMPADNFGVEPQYL
                     ITFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECGQSHGGNNDDFLFAR"
     gene            20300..20806
                     /locus_tag="OO172_001580"
     CDS             20300..20806
                     /locus_tag="OO172_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511809.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            20803..21363
                     /locus_tag="OO172_001581"
     CDS             20803..21363
                     /locus_tag="OO172_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511810.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            21372..21539
                     /locus_tag="OO172_001582"
     CDS             21372..21539
                     /locus_tag="OO172_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5511811.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDEL"
     gene            21526..23019
                     /locus_tag="OO172_001583"
     CDS             21526..23019
                     /locus_tag="OO172_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5511812.1"
                     /translation="MTSFNTIPSNTLVPIFYAEMDNSAANTAQDSGASLLIGHANNGA
                     EIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATVT
                     LTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPALPFTASSSAG
                     VVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAAM
                     ADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAGD
                     QFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKGK
                     RFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAYV
                     LRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYEL
                     FKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            23019..23375
                     /locus_tag="OO172_001584"
     CDS             23019..23375
                     /locus_tag="OO172_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5511813.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPASKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            23375..23644
                     /locus_tag="OO172_001585"
     CDS             23375..23644
                     /locus_tag="OO172_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5511814.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            23786..25621
                     /locus_tag="OO172_001586"
     CDS             23786..25621
                     /locus_tag="OO172_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5511815.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETISPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYIPYPAVDLAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            25682..27010
                     /locus_tag="OO172_001587"
     CDS             25682..27010
                     /locus_tag="OO172_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR5511816.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEENAGTGRRVETHEYPNRDKPYTE
                     DLGKVTFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKGEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDVARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVEQATTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            27007..28086
                     /locus_tag="OO172_001588"
     CDS             27007..28086
                     /locus_tag="OO172_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR5511817.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEISRQWPGD
                     GGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRELVQIAQALAAPFGIEVVNNGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEDPGARKKKKARVQEDPF"
     gene            28086..28634
                     /locus_tag="OO172_001589"
     CDS             28086..28634
                     /locus_tag="OO172_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR5511818.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCLTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            28634..29059
                     /locus_tag="OO172_001590"
     CDS             28634..29059
                     /locus_tag="OO172_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR5511819.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYASRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            29046..30104
                     /locus_tag="OO172_001591"
     CDS             29046..30104
                     /locus_tag="OO172_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR5511820.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDEFWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRLPIACSTAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWAY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            30095..30679
                     /locus_tag="OO172_001592"
     CDS             30095..30679
                     /locus_tag="OO172_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR5511821.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSARDPAIAGAAPSLTRVHQRAD
                     VLMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPVATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            30683..31333
                     /locus_tag="OO172_001593"
     CDS             30683..31333
                     /locus_tag="OO172_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511822.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            31305..31739
                     /locus_tag="OO172_001594"
     CDS             31305..31739
                     /locus_tag="OO172_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HCR5511823.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYLTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(32512..>32930)
                     /locus_tag="OO172_001595"
     CDS             complement(32512..>32930)
                     /locus_tag="OO172_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5511824.1"
                     /translation="GFIVFSITAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAIN
                     TIITTVGLIIFNIFIAKIICGNDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVT
                     IPNPICAIIYTCIYVYVSDKIGHWLSRTVGGPFLIK"
BASE COUNT         8133 a         7748 c         9104 g         7945 t
ORIGIN      
        1 ctgggggtaa tccgtatttc tcacggatta cggagtctat tttttcttcc atttgtttat
       61 agtcaggaag aaggcgtttc agtggtgcgg gaatgtcctg gcaatacgct tctgttgcat
      121 catgcattaa cgcttcaaaa gcaaattcct gcggcaccag ctggctgcaa agaaccgcat
      181 gttgggcgac gctgtagaag tgcgaaagat gaccggcaaa gcgacagata tttgaaaggg
      241 aaaccgcgat atcgttaata tcgatgtcgt ctttatttat cctgtcataa taaaaatgct
      301 tcccggaaaa agttttaata aatgacattt tgttctccac gtatatgcgc tgcaccgcgc
      361 tgaattctgg taaaaagaat ccctcaccat ccggcgatta ttgagtaaat tacgtttcca
      421 taaatgcccc cgcaggggca tttgcagtaa tgaaatcagg cggtgaaagt accaataaag
      481 gtttctactt tgctgtcctt gaatttctca acaagcagat cacgaaattc gttagccatt
      541 tcttcctgca ccgcctccag ctgaataatg cgcagaacca gtaccggacg atcgccagtg
      601 ataatgctga ggcgtaattt aaacggacgt tctttcagac cttcaaacgg aatgcattta
      661 aattcaaatg ccactggcat aatgtctttg gtcttcgctt cgacagactc catcagggag
      721 cgtttgccgc tgaagtcatt atcttcaaaa tcagcggtct ggtttgcttc aatcgtgatt
      781 ttacggaccg ccgcagccgc ttttgttgcc tgaatggtgt caccattagc atcaaagccc
      841 acaaggtagt cggcccagtc ttcaatccat tctgccagtg atttctggga gttacgctcg
      901 ccattaacag acaacagagc agagaacggt gctgtctttt tcagtttgag agtggcggtg
      961 ttatctgcgt gacctggttc atcaatagta cccaggttaa gcacactgac ggcacgcata
     1021 ttatcagcat cgataaagca gcgggtgcct tcatctgcaa gatctttaga ataacgggta
     1081 aagtcatcga tgctggcagt ggaaagcgca ccacggaaac ggaagcgatt taaattaaat
     1141 ttttccagat catgaatgcg gaaattctca ggcaatgcca cagcatcggc accaatctta
     1201 ctgataattt cattaacacc ctgagcagaa ataagggcat ggatttgatt aattgcggtt
     1261 gcgtctaagt tctgagacat aataagtcct aactatataa agatattcag tgatgagata
     1321 aataatcagt taattaagaa cgatattaat gacctgctgc gcggagtttt ccgtcaggtt
     1381 caccggcaag agtcagtaat tgtccctggt cttcctgcag aatagtcagg cgaccaccgc
     1441 gattgacata catcggcgtt tcggtggtgt cttcttcgga aattttcccg cggttagtcg
     1501 ggcgaacata tgagagtttg tgtttgattt tcacacggtt ctcatcaaat ggttcgattt
     1561 ccaggttgag tgagacctta cctttggttt tcgtgttcat cacaccggaa gcgacttcac
     1621 tgagaactgc gccgattttg gtttcaaata cgccgccgtc cagctccccg ataaatgcct
     1681 gcacatcagt actgcgttcg ctagccattt tgctgctcct catcatatcg accctgcaag
     1741 gccgattagt ttctccacaa aacagagaag aacacctgcg gtagcagccg cccggatgga
     1801 ttgggttatg agcccgtcgt ccggtgatgc tcttctctgt tttgtaaaaa ggacggtacc
     1861 agccggaagc aagggtacaa actggtaccg ccaggactac acacagcata aagttgtggt
     1921 gccgggtgcc tcccggtgcc tggcgaaggt tgcacaccag acgggtgggt atccacagaa
     1981 ggtcgactgt cagcctcaac cttaacccgc gtgcgctgag ccgcattcac cacaactcta
     2041 aggattctct ttggttgaaa atacttagct gttatgtgcc tgtcttttca ccacttcagg
     2101 ctcggtggta tccttttaag cccgtataca taaaaggaaa atcaaatgac ttttgatgaa
     2161 aaagaacttg ataatgcaat taataaaatc atcgtaacgt cgctcttttc ctgtctcagc
     2221 gacactcagc agaaacagtt ctacgaatcg gctttcaaca tgatcgagcg ttgttgtttc
     2281 tgcgatgccg acgagttacc tgaaaaaatc aggaaacagt tggctgatgc tcttcgagtg
     2341 cgactttctg accaattttc tgaaatgtgc tctccgaatt tggacaaata gaaaaaggcc
     2401 atttccattc agggtctgat ggaaatactt cagcctgttc caaagcacgg cgtaaagaga
     2461 acacaactcc agccataatc tgatgtttcc cattggtcca gctatcgccg ctctgatcta
     2521 caggggcggc tatgtcgtat gaccaaacga cttcacagtt attgtttaaa atctggactt
     2581 tcatttcata cacctgcttt aacatgagtg cctagtggca caacatgact caacgaatca
     2641 tcctggactt catatgcccc aggcggctac ttcgtgggcg tcctgcctgt tcgttgtttc
     2701 gcttgggtac attatgtatc ccaagggtac attgtcaagt ataaaaaaac ctgccgaagc
     2761 aggttcataa acattgatta ggctttgatt ttgtatcttc ttggttttcc tgagaaaatc
     2821 actgtaccaa ttatagagca attaccgttg atcttaatgt aaggctcagg ccagtttggg
     2881 tttaacgctt tgagataacg ctgtgtccca tcttctatca accttttgaa ggtggtttcg
     2941 cctgtatcgt gcatcaatgc aataacgtcg tcaccgtggc aggcaggtac ttcaggatcg
     3001 acaaaaatca tgtctcccgg gcggtactca tcaatcattg aatcacctat cacccgcaag
     3061 atataagtca tttccccaca gggtacaggg cagggatacg tttctgctgt gctcaaatca
     3121 acctcagaat atccaacttc tttccatgct ccggcctgta cccatgatat gacagggact
     3181 aatgtgattt gtttattagt gattgaaaca tcaggttttt ttgtgatgtt cgttgtctgg
     3241 tgttcttgat cgagccatcc gacaggcagg tcgaaacatt tttcgatgtg tcgtgccatg
     3301 ctgtcaccga tatttttagt agcaccatct cccataaacc tgctggtctg ggttggctcg
     3361 cgatcaatca tagtggcaaa ggaagaattc ccgccaacac catctctcag ttttctggcg
     3421 ttagaccgcc ggatgtcatg gattgttttc ataacgaaat taaaaccctt gtaccgttaa
     3481 ggtacaagtg tcttggaggt tcatttcaat catgtaacat gtacaccgga ggtacatatt
     3541 gtatgaaagc gtattgggac tctttaacca aagaacagca gggcgagttg gccggaaaag
     3601 ttggctcaac acctggctac ttacggctgg ttttcaatgg ctataaaaaa gccagttttg
     3661 tgctggctaa aaaacttgag caatgcacgt caggtgcaat tacgaaatct gacttaagac
     3721 cggatatcta tccgaaagat tagcagaaca ctttcaattt ttaaccacag aacgatgagg
     3781 ctaaccgtgg gtaagcatca ctggaaaata gaaaaacagc ctgagtggta cgtgaaagct
     3841 gtcagaaaaa ctatcgcggc gttgccgggt ggttacgctg aagcggctga ctggctcgat
     3901 gtaacagaaa acgctttatt caaccgcctt cgtgcagatg gcgatcagat tttcccgctg
     3961 ggatgggcaa tggttttaca gcgtgctggt ggcactcact tcattgctga tgctgtggcg
     4021 cagtctgcaa atggcgtctt tgtgtctctt cctgacgtcg aggatgtgga caacgccgat
     4081 atcaaccaac gcctgctgga agtcattgaa cagatcggca gttattcaaa acagattcgt
     4141 tcagcaattg aagacggtgt agtggaaccg catgagaaga cagcaattaa cgatgagcag
     4201 tacctctcaa tttcgaagct gcaggagcat gcagcactgg tctacaaaat cttttgcgtt
     4261 tcagaaagta gtgacgcccg cgagtgtgca gctccgggcg ccgtggcatg tcgtgactgt
     4321 ggagaaacta acgcatgaac agtttaacaa cacactaccg tcgctcgcaa ctgattgcgc
     4381 ttcctgtacc gggtggaaaa gcgaaggtgg agtattgcta tgcagtaaat gtaccaggtg
     4441 acagggaaat tgtaacccac agctttgcag agtgggctgt gggtgatttc aaccggcaga
     4501 aggagacagt cctttgcgac aagttaaccg ctggttcaaa gagcactacg gagtgcccgt
     4561 cagagtcatt cgttgggagc cggaaacaca acgtgttatc tacctccgcg aaggctatga
     4621 gcatgagtgc ttcagcccgc tcgaacagtt tcgtcgtaaa ttcagggaaa tagaggtcgg
     4681 tcatgagcac taaattaacc ggctatgtat gggatggttg cgctgcatca ggcatgaaat
     4741 tatccagcgt ggcaattatg gcccgcctgg ctgatttcag taatgacgaa ggtgtgtgct
     4801 ggccatcaat tgaaaccatt gcccgccaga ttggcgcggg aatgagtacc gtcagaacgg
     4861 ctatcgcacg gctggaagca gaaggctggt taacgcgtaa ggcgcgtcgc cagggtaacc
     4921 gcaatgcgtc gaatgtttat cagcttaacg ttgcgaagct tcaggcagcg gcattttctc
     4981 aactgtcaga ttctgacccg tcaaaatctg acgcatcaaa atctgacccg tcaaaatttg
     5041 atgcgtcgaa atctggcaaa aaagcgggtt ttcacccgtc agaatctggc ggggatccgt
     5101 cagtaaaatc aaaacatgat ccgtcagata aaaaaacttc tcgtccggac gcttcgcaac
     5161 cggacacgca gacggctgaa caggagtttt taactcgcca tcctgatgcg gttgtattca
     5221 gccctaaaaa gcgccagtgg ggaacgcagg atgatttgac ctgcgcacag tggctctgga
     5281 aaaaaatcat cgccctgtac gagcaggccg ccgaatgtga cggcgaggtg gttcgtccca
     5341 aagaaccgaa ctggacagcc tgggcaaacg aaattcgcct gatgtgtgtg caggatggtc
     5401 gtactcataa acaaatctgc gagatgtaca gccgcgtcag tcgcgatccg ttctggtgcc
     5461 gtaacgtgct cagcccgtcg aagctgcggg aaaaatggga tgagctttcc ctgcgcttat
     5521 cgccgtccgt cagcacgtac accgaaaaac gcgaagaccc gtacttcaaa gctagttacg
     5581 acaacgtgga ctacagccaa atcccggcag gattcagggg gtgagcatga gtcttttgaa
     5641 tgacgttcag aaattcattg aagcccatcg gggctgtact tccggagata ttgcggatgc
     5701 ttttgccggt tactcacggc agcgcgttct gcagtcagca agcaagttac gtcagagtgg
     5761 gcgtgtggct caccgttgtg aaggagatac acgcagacat ttcccgcgcc tgactgagag
     5821 agcgcaggaa ccggaaccac aaccagttcg tgaaaccaga cctgtgcgca atttctatgt
     5881 cggcactaac gatccccgtg tgattttgtg cctgacccgc caggcggaag aactggagtc
     5941 caggggctta taccgtcgtg ctgcaaccgt gtggatggcg gcattccgtg aaagccactc
     6001 ccagccagaa cgaaacaatt ttctggcgcg tcgtgagcgg tgcttacgga aaagcagcaa
     6061 gcgcgctgca tcgggtgaag agtggtatct gtcagggaat tacgtggggg cttaatgagt
     6121 aataaatatt gccaggcgct ggtggaactg cggaacaaac cagcccatga actgaaggaa
     6181 gtgggcgatc agtggcgcac gccggacaac attttctggg gaattaacac cctgtttggc
     6241 ccgtttgttc tggatctgtt cactgacggt gataacgcca aatgtgccgc gtattacacg
     6301 gcggaagaca acgcgctggc gcatgactgg tcagaacgtc ttgcggagct taaaggtgct
     6361 gcctttggta atcccccata cagccgcgcc agtcagcatg aggggcaata catcaccggc
     6421 atgcgttaca tcatgaaaca tgccagtgcc atgcgtgata agggcgggcg ctatgttttc
     6481 ctgatcaaag ctgccaccag cgaagtgtgg tggccggaag atgcagatca tattgctttt
     6541 attcgcgggc gtattggttt tgaactgcct gtctggttta tcccgaaaga cgagaagcag
     6601 gtactgacag gagctttttt cgctggtgct attgctgttt ttgacaagac ctggaaggga
     6661 ccggcaatca gctacatcgg gcgcgatgaa cttgaggcat gtggtgaggc gtttctggcg
     6721 caggttcgcc agcaggcgga aaaactggtc agggagatgg cggcatgacg acgttaactc
     6781 aatgccagca gcaggtgctg gatatgctga tttcttatca gaaagaacgt ggcttcccgc
     6841 caaccaatca ggaggtggca accatgctgg gataccgttc ggtgaatgca gcggtggagc
     6901 atcttcgcgc actggagaaa aaaggcgtca tcacgataaa gcgtggcgtg gcccggggga
     6961 taacgcttca taccgcggtg aaggacgacg acagcgaggc ggtcgggatt atccgcgcac
     7021 tgcttgccgg tgaggaaaac gcaaggctgc gtgcaaccca ctggttacat gagagggacc
     7081 tgaaagtatg aagctgatcc tgccttttcc gcccagcgtg aacacgtact ggcgacaccc
     7141 caacaaaggg gcgtttgctg gtaagagcct gataagcgcg gcggggcgaa aattccagag
     7201 cgcggcgtgc gcagcaatag ttgagcagtt acgtcgtctg ccgaaaccaa cgtcggcacc
     7261 tgcttcagtg gagatcgtgt tgtttcctcc ggataaccgg atccgcgatc tggacaacta
     7321 taacaaggcg ctgtttgacg ccctgaccca cgcgggtgtg tgggaagacg acagtcaggt
     7381 gaaaagaatg ctggtggagt gggggccggt tatcccggaa gggaaggtcg agatcactat
     7441 caataagtac gagaaaacgg cgggtgcagc cgcctgatta agaggagaaa cgaagtatga
     7501 ataatctgat ggtcattgat ggtattgaag ttcgtcgtga tgcttatggg cgttacagcc
     7561 tgaacgatct gcacagggct gccggttctc aggataagca taagcctgca ttctggctcc
     7621 gcaatgagca aaccgaacgt ttaataagcg agttgcagat ttgcaactcg gtcaatatag
     7681 agccagttaa cgttagtcgt ggcggaaata accaggggac gtatgtctgc aaagaactgg
     7741 tgtatgccta tgcaatgtgg atcagcccgt cattccatct gaaggtgatc cgtactttcg
     7801 atatggtaac cagcgcaccg gaaaaattat ccgggcaggc tgctgacaag atgcaggctg
     7861 gcgtgattct gctggacttt atgcggcggg agttaaacct gtctaactca tctgtgcttg
     7921 gggcctgtca gaaactccag gaggctgttg gcttaccgaa tctggcacca cgctatgcca
     7981 ttgatgctcc tgctgacgcg cctgatggct caagtcgccc tacgctgtcg ctgagtgcac
     8041 tgctgaaaca gtatggtatc cgcctgacag ctaatcaggc atatcaccag atggcgaagc
     8101 tggggatcgt tgaacaacgc gaacgataca gccgtaccgc gattaacaac atcaaaaaat
     8161 tctggtcgct gacggcgaaa ggctgcatgt tcggcaagaa cattaccagt cctgcaaatc
     8221 cgcgcgagac gcagccgcat ttcttcgaat cccgattccc tgagctgtta aagctgctcg
     8281 ataccgttca ttgaggtgac cgtgagagca ctactgaccc ctgaaattgc cccgcgtatg
     8341 gggatcgtat tgttcagacc aggttcagag ctgatgcccc tgtttatgca ggggcgtgta
     8401 ctgctggagc ctgagccgga acgttattca tctttcgcca gtggtgccgt tcccgcggca
     8461 tcacaaccgc tggcggatga tcctgctgtt cgggccgtgt tccgcaatga ggcagtgatc
     8521 cgtcgtgctg gtggcgtgga atgtcttgaa agctggctac ttcgtgaaaa aggctgccag
     8581 tggcctcatt ccgactggca cagcgagaac atgaccacaa tgcgacacgc tccgggcgca
     8641 atccgtctgt gctggcactg tgataaccag ctgcgcgatc agttcacgga acggctggaa
     8701 tcaatggcaa cggataactg tgcccgctgg gtgttgtctg ttgtgcgtcg ggatctcggt
     8761 tttgatgata gtcacgttgt gacaatgccg gaactgtgct ggtggctgat tcgtaatgac
     8821 ctggcggatg ccttaccgga aagtgcagcc cgtaaggcac tgagattacc aaagcctgtt
     8881 gtgccgtctg tcacccggga aagtgacctt gtgccttcgg ttcctgccac cagcatcatc
     8941 caagataagg caaaaaaggt gctggcgctg aaagtggatc cggagtcgcc ggagtctttt
     9001 atgttacgcc caaaacgtcg ccgctgggtt aatgagaagt acacgcgctg ggttaagaca
     9061 cagccgtgtg catgttgtgg aaagcccgct gatgatcccc accacctgat aggccacggt
     9121 caggggggaa tgggtacaaa agcgcatgac ctctttgtgt tgcctttgtg cagaaagcat
     9181 cacgacgagc tgcatgcgga taccgtggca tttgaagaga agtatggctc ccagctggag
     9241 ctgatatttc gttttatcga tcgtgcgctg gcaattggcg tgctggcctg atttggtgga
     9301 gaaagttgat gcgtgatatt cagatggttc ttgagcgttg gggagcatgg gcggcgagtg
     9361 atagttcagg cgttgactat tcacctatag ctgctgggtt taagggactt cttccctata
     9421 ccagcaagaa acgcttggct tgttcggata gtgatgcatt aattattgaa ggttgtcttg
     9481 ctcgtctaaa gcaaaaaaga ccttatgagc attcgcttct tgtggcacat tatttataca
     9541 gaatatccaa gcgtaagatt gcaaaggcgc gtggaaagga tgagaaattg atacgtatag
     9601 aaatacagct agccgaagga tttattgatg gctgcctttc catgctagat ctaacattag
     9661 atttggacgt ttaataatac gccccatgca ggggcgtatt atttactgga tgaatgacat
     9721 ttgattaata tattttatca ataactctct gggtgtagta ctccaaaaat ttaggtgttc
     9781 ataatcagta taaacagtgg tgaatttttt aagttcttca tcacttttag gggcaaatcg
     9841 ctcattaaga gtaatactgt ctttaattat tccgaagaat acaatagata tttcaggaat
     9901 ttgcgatctt ctctcataag aataaggtat ttcttttatg ttaagcagat ctgctaaatg
     9961 agatatagat tctgcggtaa tttcttgttt tagtttttcc ttgtcaccaa agcaatgtat
    10021 gaagacagct acgacaaaga tcttatttat taaaggatca ctgcttttgt actcatcgtc
    10081 gtttaatagt tgaaaaatgt taagattgcg tgaaaaagtt tgtgtttcac gtaaggataa
    10141 gttggttcgt tgaattaaat cacggataaa tctaccagat aacctattaa tttcatttaa
    10201 tgattttgtc ttgtctacaa ggtaattcca atatataaca gatgttttac atacgacgtg
    10261 accatttatt aaacatgtgt ctggaagcgt gatggtatat tttataaact tgtcaagata
    10321 cttttgtgag ttaatactat aaccataaat atgatttatc gatgctttta attgttctgt
    10381 gtttgtaact aaaataaaaa agacattatt gatgtcaaaa atgtgtttta ttgtttcaat
    10441 gacatttgtt gaaaaactcg gcttacatcg gtctaattca tcaattataa ttactatctt
    10501 ttgactttct gaaatgcttt cgatgcaaga tttaagtgaa tttatgtttt tctctgattc
    10561 catatggtct tcaagcaaat tttcaatagt cccatctatt gctgcattgc ttgctttctt
    10621 catcgcatct tggaattctt cggaaacttc actagcctcc tgtcgtaaaa accaacctgc
    10681 accagctttt agtaccgttt ttaaaccaaa gcgaattgca ggaagagatc tcttaatgaa
    10741 gtgttgtttt tcttcctcag gtaaaatgct ggcaattgca gaggttatga gaagtaatgg
    10801 agattctgca tgatcccctt taaaggcatc aatataaaca actttagatt cagtttcttg
    10861 ctcaataatg agatttttca gtttgatact aaattctgtt ttccctgttc cccatgcgcc
    10921 gtctattacc agtggtgaaa tgtctgcctc tggttttagc aacttgatga tattttcagc
    10981 gatgtttctg cgttggaact cgtcacgttc agtgaaagat agtgtatcta acataatata
    11041 aacctattaa ctcctacagt aaaaaacgaa taatacgact ttgggattaa aaatcattaa
    11101 cgcggtccgc aaaaattctt gtaatctgtt aagagtggtt acttcgccac acagcttaaa
    11161 cccgccgtcg agcgggtttt gtcgtttttg ttgctgggga ttcgttgggc ctggcctatc
    11221 ccgcagttat ccattggttc ggctctttga cgtttccgct tctgatttgc ggtacatgat
    11281 gttccctcaa tttgcacctg ctgtatcagc gaggtgagag ataactacaa atgcctcata
    11341 acccaaatac ctggttggag ttggtccaga gctggtggcg tggagacaca ccgctgggcg
    11401 cagtgattat gtcgattgtt atggctggct tgcgcattgc atattttggc ggtggtgggg
    11461 gctggaaacg aaaaacgctc gagattttgc tctgtggtgc tctgacgctg acttttgcat
    11521 ccgctcttga gtatgtcgga tggcctaaat ctctttctgt tgccattggt ggcggcgttg
    11581 ggctaatcgg ggtcgatgct attcgtgggg ctgcaatgag agtaatcggt aataagtttg
    11641 gtggctctaa ggagtaattt atgcaggtac taaattccca gcgtaaagct ttcctcgata
    11701 tggtggcatg gtcagaagga acggataacg ggcgacaacc gacacgtaat cacggttatg
    11761 atgttattgt tggcggcgaa ctgtttactg attactctga tcaccctcgc aaacttgtca
    11821 cgctaaaccc caaactcaaa tcaacagccg ccggacgtta tcagcttctt tcacgctggt
    11881 gggatgccta ccgtaagcag cttgctctga aagacttctc ccccaaaagc caggatgctg
    11941 tggcactgca acagattaaa gagcgtggcg ctttaccgat gattgatcgc ggtgatattc
    12001 gtcaggctat cgaccgttgc agcaatatct gggcgtcgtt acccggtgca ggttatggtc
    12061 agtatgaaca taaaatcggt gatttgattg cccggtttaa agaggctggt ggggtggtaa
    12121 atgaagctga gctataagct gcttatcgtt ggtttcttcg ttactgtcat tggttctttc
    12181 atctggtcgg ccaaccacta ctacagcaaa tatcagtacg aaaagaaacg tgctgatgag
    12241 gctgtacgaa atgctgaatc agcaactgcc attaccaata acgtcctgca atcactacaa
    12301 atcgtcaata cagttctgga ggctaaccag catgcaaaac agcagatcgc actggagtca
    12361 cagagagccc gggaagatat caaagtggct gttgcggatg atgattgtgc ttcacgccct
    12421 gtgcctgatg tcgctgctga ccggttgcgg aagtacgcgg acagtatacg tgcaggttcc
    12481 aacgatgccg ttaccgacga acctgctcgc tgaaactcca cagccagtta tacccaatcc
    12541 tctgacttat ggggatagtc ttagtttgaa tgtaagtctg ctatcagcac tggggctatg
    12601 taaccgtgat aagtctgatc ttcgtaggtt aggagagcaa aagtacaatc tgcatttgaa
    12661 taataatatt cattaggtga aatattatta tttgactgtt ctagttatta tgcatttagt
    12721 tacaatactc tcactattaa cagtgaggta aaaatgaacg aaaattatat tgcatatgag
    12781 acacttgtag caaaccgtgc tgctgctgag tgggcttgtt gggcaatgat tgcatcttgg
    12841 gtgagtgctg gagctactat tgttactttg gttttggcgt tcaaggcatt gtttacgtgg
    12901 cgggaacaag agaaaacaaa agtaaaaata gattttagga atgcattaaa gaaattaaag
    12961 acagctctat tatttatgcc tgtcaatatt gaccccgaga aactcaatga tgaacgagag
    13021 caagttattg cgaaatggct atttaaagat gtagatctta ttagtcagca aattgagttg
    13081 ggagaagaga atgttaaaag atttgatgaa cttttgagta ttttcgattg ttgccagtct
    13141 tcatggtttg cgacagagca cttatttgat aatactgagt tagaaaaagt ttggcatgag
    13201 ttcgagtcta actttaataa atatataaat ggtggtgaga gtaaggattt acttatgaaa
    13261 atgcttgata agctcatctc ttctagattt gtatttgagt caagataatt gcctttgagt
    13321 atttttcttt attattttac ttattataaa ttttttatat gccccctaga atcccaaaag
    13381 cctgccgtgt tcgcggctgc cgctctacaa ccacggaccc gtcaggctac tgcgaaagcc
    13441 ataaaagcga aggctggaag caatacaagc caggacaatc tcgtcatcag cgcggttatg
    13501 gttcgaagtg ggatgttatc cgcgtgcgtg tcctgaagcg tgacaaaggc ctgtgtcagc
    13561 tatgtctgcg tgctggtgtg gtgcgtgagg cgaaaactgt tgaccacatc atccctaaag
    13621 cgcatggcgg cacagatgcc gacagcaatc tgcagagcct gtgctggccg tgccataagg
    13681 agaagacggc ccgtgaacgg ctaaagtgat cataattctc aactgtctga ggggaggggc
    13741 gggtcaaatc tctgtgacct gacgtcttcc ggactgcccg ccccatcgtt tttttatacc
    13801 cgcgaaaaat gaaatttaac caggagtgcc gcatatggct ggaacggcgg ggcgttccgg
    13861 gcgtcgccct aagccaacgg cgcgcaaggc gctggccgga aaccccggca agcgagccct
    13921 gaacaaagat gaacctgttt ttacgcccat caaaggtgtt gagccaccgg agtggttcgc
    13981 tgaagaagag cttcctctcg ccacgatcat gtggcaactg acaaccaaag aactctgcgg
    14041 tcagggcctg ctgtgcgtga ctgaccttgc ggtgcttgag cggtggtgtg tggcctatga
    14101 gttctggcgg cgtgccgtga aaaatattgc cagacagggc aacaccatca ccggtgcaat
    14161 gggcggtatg gtcaaaaatc cggagctgac cgccaaaaaa gaacaggagt ccgagatgag
    14221 cagcacgggg gcaatgctcg gactcgaccc cagtagccgc cagcgtctga ttggcctggc
    14281 ggggcagaag aaagccacta acccgtttct gaaaatcatc gagtcatgag ccggaaatct
    14341 taccccaacg taaatgctgc caatcagtat gcccgtgatg tcgtgcgcgg aaagattgtg
    14401 gcctgccagt ttgtgattca ggcctgccag cgccatcttg atgacctgat ggcggaaaaa
    14461 agtaagtcgt ttcgttaccg cttcgacaag gagctggctg aacgggccgc gaaatttatt
    14521 cagctgttgc cacacaccaa gggggagtgg gcattcaaga ggatgcccat cacgctggag
    14581 ccgtggcagc tctttgtgat ctgctgtgcg tttggctggg tcaataaagg gtcccggctg
    14641 cgccgcttcc gggaggtgta taccgaaatc ccccgtaaga acggcaaatc ggcaatctct
    14701 gccggtgttg ccctgtattg ttttgcctgt gataacgagt ttggcgcgga agtgtattcc
    14761 ggtgccacga cagagaaaca gacgtgggaa gtctttcgcc cggcgcgact gatgtgtaaa
    14821 cgcacaccca tgctgacgga agcgttcggg attgaggtta acgcctcaaa catgaaccgt
    14881 ccggaggatg gggcgcggtt tgaaccgctg attggtaacc ccggtgatgg ttcatcaccc
    14941 cactgtgcgg tggtggatga atatcacgag cacgccacag atgcgcttta caccacgatg
    15001 cttaccggga tgggggcgcg acgtcagcca ctgatgtggg ctatcactac cgccgggtac
    15061 aacattgagg ggccgtgcta cgacaaacgg cgggaagtca tcgagatgct caacggttcg
    15121 gtacccaacg atgaactgtt cgggattatc tataccgttg atgaaggtga cgactggacc
    15181 gacccgcagg tgctggaaaa agccaatcca aatattggcg tgtcggttta tcgcgaattt
    15241 ttgttaagtc agcagcagcg tgcgaaaaat aacgcccgtc tggcaaacgt ctttaaaaca
    15301 aaacacctca atatctgggt gtcggcgcgt tcggcgtatt tcaacctggt gagctggcag
    15361 agctgcgagg ataaatcact gacccttgag cagttcgagg ggcagccgtg cattctggcc
    15421 tttgacctgg cgcgtaagct ggatatgaac agcatggcgc gactttatac ccgcgagatt
    15481 gacggtaaaa cgcattacta cagtgtggcc ccgcgtttct gggtaccgta tgacacggtg
    15541 tacagcgtcg agaaaaatga agatcgacgg acagccgaac gctttcagaa atgggtggaa
    15601 atgggcgttc tgaccgttac cgatggtgcg gaggtggatt atcgctacat cctcgaggag
    15661 gccaaagcgg cgaacaaaat cagtccggtc agtgagtcac ccatcgaccc cttcggggcg
    15721 accgggttgt cacatgacct tgctgatgaa gacctgaacc ccatcactat cattcagaac
    15781 tacaccaaca tgtccgaccc gatgaaagag ctggaagcgg caattgaatc ggggcgcttt
    15841 catcatgatg gcaatcccat catgacctag tgtatcggca acgtggtcgg caaaaccatt
    15901 ccgggtaacg atgatgtggt gaagcccgtc aaagagcagg cggaaaacaa aattgacggt
    15961 gcagttgcgc tgattatggc ggttggcaga gccatgctgt acgagaaaga agacacgctg
    16021 tctgaccaca ttgagtccta tgggatccgc tcgctttaac tgaggtaatt atgatcatgc
    16081 tgattctcgc gcctctggtg ggcgtgctgg gggcgctttt gctggcgtat ggtgcctggc
    16141 tgatttatcc cccggcgggg tttgttgttg ccggggcgtt gtgcctgttc tggtcgtggc
    16201 tggtggcgcg atatctcgac cgtacacagt cgtctgtcgg cggaggtaaa tagtgttctt
    16261 ttcgggatta tttcaacgaa aaagtgacgc accggtgacc acgccagcag agctggcgga
    16321 tgatatcggg ttgtcctacg acacctatac cggaaagcag atcagcagcc agcgggccat
    16381 gcgactgacg gcggtttttt cctgtgtcag ggtgctggcg gagtcggtcg ggatgttgcc
    16441 ctgcaacctg tatcacctga acggcagcct gaagcagaga gccactggcg aacgtctgca
    16501 taagctgatc tccacgcatc ccaatggcta tatgacgccg caggagttct gggagctggt
    16561 ggtcacctgt ctgtgcctgc ggggaaactt ttacgcctac aaagtgaaag catttggcga
    16621 agtggctgaa ctgctgcccg tcgatcccag ctgtgtggta ccgaagctta acagtagctg
    16681 ggagccggtc tatcaggtca cattcccgga tggctccgcg gatgtactga gccaggagga
    16741 tatctggcat gtgcgcacgc tgacgctgga cggactggtg gggctgaatc ccatcgccta
    16801 tgcccgcgag gcaatatcgc tggcggcagc gaccgaagag cacggggcca gactgttcag
    16861 caatggcgcg gtgacgtcgg gtgtgttgcg tacagagcag acgctgtcag atcaggctta
    16921 cgagcgcctg aagaaagatt ttgaggagcg tcacaccggg cttggcaatg ctcaccgccc
    16981 gatgatcctt gagatggggc tggactggaa gtcgatggcg ctgaacgccg aggacagcca
    17041 gttcctggaa acccgcaagt ttcagcttga agaaatctgt cgtctgttcc gggtgccgtt
    17101 gcacatggtg cagaacaccg atcgcgccac tttcaacaat attgaagagc tggggctggg
    17161 atttatcaac tattcactgg tgccgtatct gacccgcatc gaacagcgga tcaacaccgg
    17221 actggtacga aaaagtaagc agggcgttta ttacgccaaa tttaacgccg gggcgttact
    17281 gcgcggggat atgaagtccc gttttgaagc ctacgccacc gggattaact ggggaattta
    17341 ctctcccaat gactgccgcg acctggaaga tatgaatccg cgtcccggtg gtgatgtcta
    17401 tctcacaccg atgaacatga ccacgaaacc ctccgatggc agtaaagccg gtaagcagaa
    17461 ggataacgcc aatgcagacg aaacaacgtc ttgatgtacc gctgagtctg aaatctgtca
    17521 gtgactccgg tgagtttgaa gggtatggct ccgtctttgg tgtaaaggac agccacgatg
    17581 atgtggtgat gtccggggca tttgccgctt ccctgcgggc gtggagtgac agaaaagcgt
    17641 tacctgcgct gctctggcag caccgcatgg atgagcccat cggtgtttac accgaaatga
    17701 aggaagacga tgtcgggctt tacgtcaggg ggcggttgct cattgatgat gatcccctgg
    17761 caaaacgcgc acatgcacac atgaaggccg gttcgttaac cggcctttct attgggtacg
    17821 tcctgaagga ctgggaatac gaccggacga aagaagcctt tctgctgaaa gaaatcgacc
    17881 tctgggaagt cagtctggtg acgtttccgt ctaacgacga ggcgcggatc agcgacgtca
    17941 agaacgcgct ggcccgcggg gaaatccccg aacagaaaaa aatcgaaaga gtcctgcgtg
    18001 atgtcggact ctcccgtacc caagccaaag cattcatggc cgggggctat atcgcactgt
    18061 ccctgcgcga cgctgaggat gtgggctctg cactgaatgc actgaaaaat ctgaacttct
    18121 aatcaggaga aatacgatgg cggtagatat taaagatgtc gaacaggtcg cgcaggagct
    18181 gcagcagaag tttgacgact tcaaagcaaa gaacgacaag cgcgtggatg cgattgagca
    18241 ggaaaaaggc aagcttgccg ggcaggtgga aaccctgaac gggaaactca gcgagctgga
    18301 aaatctcaaa agcgatcttg aaaaagagct gcttgagctg aaacgtccgg caggtggcgc
    18361 gcaaaataaa ctggccaccg agcataaaga agcgtttgtg ggctttttgc gtaaaggccg
    18421 tgaagatggt ctgcgcgatc tggagcgtaa ggcattgcag gtgggcaccg atgaagacgg
    18481 tggctatgcc gtgccggaag cgctggatcg caacattctc accctgctga aagatgaagt
    18541 ggtgatgcgc caggaagcca cggtgatcac cgttggcggt tccgactaca aaaaactggt
    18601 gaatctgggc ggcacggctt ccggatgggt gggggaaacg gatacgcgat cccagactgc
    18661 cacctccaga ctggagctga ttgaacctct catgggggaa atctacggca acccgcaggc
    18721 cacccagaaa atgctggacg atgccttctt caacgtggag gcctggatca acagcgagct
    18781 ggcaaccgaa tttgccgaac aggaagaaat tgcctttacc accggcgatg gtaccaagaa
    18841 gccgaaaggg ttcctggcgt atgaatccac tgatgaaacc gataaggtcc gggcgttcgg
    18901 caaacttcag catattgtat ccggcgaagc gacggcggtg accgcagacg ccattatcaa
    18961 actgatttac acgctgcgta aggcacaccg caccggcgcg aagttcatga tgaacaacaa
    19021 cagcctgttt gccatccgtc tgcttaaaga cagcgagggt aactatctgt ggcgtccggg
    19081 gctggaactg gggcagccgt cctctctggc gggttacggt atcgctgaaa acgaacagat
    19141 gccggatatc gccgctgatg cgaaagccat tgcatttggt aacttcaaac ggggttacac
    19201 catcgttgac cgtatcggca cccgcattct gcgtgacccg tacaccaata aaccgtttgt
    19261 cggtttttat accaccaagc gcaccggcgg gatgctggtc gattcgcagg ccatcaaact
    19321 gctgaagatt gcagcggcgt aatcactcag gggcgcggaa ccgcgccccc tgttctgacg
    19381 ggtgaagaat catgatcctg aaacaagatc tgaaatggtc accggacggt atgcgtgttg
    19441 aggtcattcg ggccggtgag tatgacgacg gggcgcttcc tgcccgggtg caggagattg
    19501 cacttcaggc cgggttagca gagcgcggaa ccagtgcaaa aagcagtaaa gcgacaaaag
    19561 agaaaaaagc cacgaccagt aaagagggct gagtatgctt ctgacaatgg aagagattaa
    19621 agcccaactc cggctggatg aggatttcga tgctgatgac cgccatctgc aactgctggc
    19681 ctgtgcggca caaaagcgga cggaaacgta tctgaaccgg aagctctatg caccggatga
    19741 aaccattccg gacagcgatc cggacgggct gcacctgccg gatgatattc gtctggggat
    19801 gctgatgctt atcagccatt tttacgaaaa ccgctcgtcg gttacggaag tggagaaact
    19861 tgacatgccg cagagttttg gctggcttgt cggcccgtac aggtactttc cgcaatgaaa
    19921 attcgtcagg cgcagaccag cgcaacctac attctgccgg accccggtga actgaataaa
    19981 cgcgtcctga tccgcctgcg ggtggatatg cccgcggata actttggcgt ggagcctcaa
    20041 tacctgatta cgttccggac atgggcgaag gttatccaga ccagtgccac cacctggcag
    20101 gaaaccgcgc agaccgggga cgccatcacc cattacatca ccattcgtta ccgccggggg
    20161 attaccgctg attatgaggt ggtctgcggt gacagtgtgt accgggtgaa acgtcagcgc
    20221 gatctgaacg gggcgcggcg ctttctgctg ctggagtgta cggaactggg cgaatgtggg
    20281 cagagtcacg gaggcaacaa tgacgacttc ctttttgcac gttgattttc agcagcccgc
    20341 ggagatgcgc tttaaccgcg cccgtgtccg gcgggcgttt gtcacgattg gtcagcgtca
    20401 tatgcgtgat gcccgtcggc tggtgatgcg ccgtgcgcgg tcggcaccgg gtgaaaaccc
    20461 cggttatcag accggacgcc tggctcgttc gattggttac atggtaccca gagccagtaa
    20521 acatcgccct ggttttatgg cacgtatagc ccctaaccag cgtaatggag agggaaaccg
    20581 ccgtatcacc ggtgattttt atccggcttt tttgttctat ggcgtgaggc gaggggcaaa
    20641 gcgtcgtcgc agccatcatc gtggtgcatc cggtggcagc ggctggcgac tggctccacg
    20701 taataacttt atggtggaaa cgcttgaaaa gaaccgcagc tggacacgct attttctggc
    20761 gcgggaattg cgtaaatcac tgaagccgga gcgacgacac agatgaaact gacgcctgtt
    20821 attgctgcgc tgcgtgcccg ctgcccgtat tttgaaaacc gggtggcagg cgcggcacag
    20881 ttcaaaaatc tgccggaggt cggaaagctg agactcccgg cggcgtatgt ggtaccgggt
    20941 gatgactctc cgggagaaaa caaaagccag accgactact ggcaggagct gaaagagggc
    21001 ttctccgtgg ttgtcatact gagtaacggg cgtgatgagc gcggtcagtt tgcctcgtat
    21061 gatgtggtgg acgatgtccg gcaaatgctc tttaaggccc tgctgggctg gaacccggaa
    21121 gcgtgcggta acccgattac ctatgacggc ggcacgctgc tggatctgaa tcgtcatgag
    21181 ctgatttatc agttcgattt ttcggtcatc agcgagttga ccgaagacga tacccgccag
    21241 caggatgacc tgaacagtct ggatgaactg cgaacgctgg cgattgatgt tgattatcta
    21301 gagcccggta acgggcctga cggcgatatc gaacatcaca ccgaaataac ccttccttcc
    21361 tgagaatctt catgtttgtg aaacctgtta aagggcggtc agttcctgac cctgcccgcg
    21421 gagacctttt gcccgccgag gggcgaaatg ttgatgagaa caactactgg ctgcgccgtg
    21481 aagcagcggg tgatatccgg cgcgtgaatg aaaaggtgaa taccgatgac gagctttaac
    21541 accattccgt caaatacgct ggttccgatt ttttatgcgg aaatggataa ctcggcggcg
    21601 aatactgcac aggacagcgg agcatcgctg ctgattggtc atgccaataa cggtgcagag
    21661 attgttgcca acagtctggt gctgatgcca tcggcagact atgcacgcca gatttgtggt
    21721 gcgggaagtc agctggcgcg tatggtcgag gcttatcgcc agaccgaccc gtttggtgag
    21781 ctgtatgtga ttgccgttcc agaagccaca ggcgcggcgg caacggttac gctgacggtg
    21841 accggggaag caaccgaaag cggcacggtg aatgtctatg tgggacgtac ccgcgtgcag
    21901 gctccggtga ccaacggcga taacgtcacg acgattgcca gcagtatcca ggatgccatc
    21961 aatgccgttc cggccctgcc gtttacggct tcatcttcgg caggcgtggt cacactgacc
    22021 gcgcgtcata aggggctttg cgggaatgaa attcctgtca gcctcaatta ctacggcttt
    22081 ggtgggggcg aagtgctgcc agcgggcgta cagattgccg tggcgacggg taccgccgga
    22141 acgggtgctc cggttctcac cggcgcggtg gctgcaatgg cggatgagcc gtttgattat
    22201 atcggcctgc cgttcaacga cacggcctcc gttaacacgc tggtgaccga gatgaacgat
    22261 accagcggtc gctggagcta tgcgcgtcag ctgtatggtc atgtgtatac ggcaaagatc
    22321 ggcacgctgt cagaactggt gaccgcaggt gaccagttta accagcagca cattaccctg
    22381 gcgggatacg aaaaagagac ccagacgcct gccgacgagc tggcggcaag ccgtaccgcc
    22441 cgcgcagcgg tgtttattcg caacgatccg gcacgtccca cgcagaccgg tgagctggtg
    22501 ggtatgctgc ctgcgccgaa ggggaaacgg ttcacgatga ccgaacaaca gaccctgctg
    22561 tctcatggcg tggcaacggc gtatgtcgaa agcggggtgc tgcgcattca gcgtgatgtc
    22621 accacgtaca ggaaaaacgc ttacggtgtt gcggataaca gctacctcga cagcgagacg
    22681 ctgcatacca gcgcgtatgt gctgcgcaaa ctgaaatccg tcattaccag taagtacggg
    22741 cgtcacaagc ttgccagcga cggtacccgc tttggtcccg gtcaggcgat tgtcacaccg
    22801 gcggtgatca aaggggaact gctggcaacc taccgtcagc ttgagcgtgc ggggatcgtg
    22861 gaaaactacg aactgtttaa gcagtacctg gttgtggagc gtgatgccag cgatccgaac
    22921 cgcctgaaca cgctgttccc gcctgactat gttaaccagt tgcgtgtctt tgccgtggtt
    22981 aaccagttcc gtcttcagta ttcagaggag tctgcataat ggcccgtatc gggggaacct
    23041 gttatttcaa aattgacggt caacagctat cgctgaccgg cggcattgag gtgcccatga
    23101 acaggacggt caatgatgac atcatcggcc tggacggttc agtggaccgc aaggaaactc
    23161 accgtgcgcc ttatgtcaaa gggaccttca aggtgccgaa gaattttccg gcgagcaaaa
    23221 tcacctcgtc tgatgagatg accatcactg ccgagctggc gaacggtcag gtctatgtac
    23281 tgtcgtctgc ctggctgcac ggcgaagcga accataatgc cgaagaaggc acggtcgatc
    23341 ttgagttcca cggtgaagaa ggggattacc agtaatgaaa gagcttgagt taaagaaacc
    23401 gattaccgct catggcgaga cactctccgt actggagttt gatgagccca ccgggaaaga
    23461 tgtccgcgag ctggggtatc cctaccagat gaatcaggat gagtccgtca gacttctggc
    23521 gcatgtggta tcgaaatata ttgtgcggct ggcgaaagtg ccgcaaagct ctgtcgacca
    23581 gatgtctccg gcagacctga atgcagcggc gtggcttgtg gctggttttt tcctccaggc
    23641 ctgacggctg aataccttac tgatcgcttc tttgattgcg ccagctactg gcgcattaat
    23701 cccttcgaat tgctgaatat gccgatcagt gaaattccct tgctggtcag tcaggcaaac
    23761 aggatagagc aggagaaacg cacacatggc tgaatttgag cttaaggcgt tgatcaccgg
    23821 tgtcgacagg ctttctcccg cgctgtcgaa aatgcaaaag aaaatccggg gatttaaacg
    23881 ccaggcggaa gaagcgtcac agggtgggct ggcgcttggt ggcggactgg cagcgggtct
    23941 gacgctttcc ctgaaatctt atgccgatca ggaaaacgcc gccaccgggc tgaaagtcgc
    24001 catgatggat gcgaatggcg aggttggaaa gagctttcag gacatcaata aactggctat
    24061 tggcctgggt aaccagctac ccggtacaac ggctgatttc cagaacatga tgcagatgct
    24121 ggtgcgtcag gggatcccgg cagaaaatat tcttggcggt gtgggtaaag cgacagctta
    24181 tcttgcggta caactgaaaa aaacaccgga agcggctgct gagtttgctg caaagatgca
    24241 ggatgctacc ggaacggcgt cagaagacat gatggggctg ttcgacacta tccagaaggc
    24301 gttttatctg ggcgttgacg ataccaacat gttgtccttc ttcactaaaa ccagttctgt
    24361 tctgaagatg gtgaacaagg acggtcttca ggctgcacag agccttgccc ccatcagcgt
    24421 catgatggat cagatgggga tgaacgggga gtcggcaggt aatgccctgc gaaaagttat
    24481 ccagtccgga ttaagcgtta agaaaatcag ggacgtcaat aaaatcatgg cccgccagaa
    24541 actcggggta cagctcgatt ttactgacgg caaaggaagt tttggcggtc ttgataacat
    24601 gttcaggcaa ctggcaaagc tgcgaaaact gaccgacgtt aagcgaacag gtgtacttaa
    24661 ggcaatattt ggtgatgatg ccgaaaccct tcaggtggtc aatgcactaa tcgataaagg
    24721 aaaggatggc tacgatcaga tccagcagaa gatgaataaa caggccagcc tgaataaacg
    24781 tgttcaggcc cagcttggta cgctgtccaa cctgtgggag gcaatgacgg ggaccgcaac
    24841 taacggcctt gcggctattg gcggcgcatt ttctggtgac gccaaaaata tcacgcaatg
    24901 gctgggggag ttgggggaga aattcacgaa gtttgcggat gaaaatcccc gggttattcg
    24961 cggcgtcgtc gggcttgctg ccggtcttgc gattctgaaa ctgggattga tgggcgtggg
    25021 cagtgccatc agtatcgtca gcaggatcat gtcgatgacg ccgattggca tgattgcgac
    25081 ggcgattgcc ctggctgcgg gattaattat cactaactgg gatgttgtcg gaccttattt
    25141 taagaaactc tgggaaacca ttagtcctta ttttgaggct ggctgggaac ttctgaagaa
    25201 ggtttttgcc tggtcgccgc tggggatggt gatcaataac tggggaccgg ttgttaagtg
    25261 gtttcaggat atgtgggata agctgaagcc aattattgag tggtttaccg acagttcagg
    25321 tgacacggtc gatgccatta actcggcgca gtggggcgcg ggtgcttatg atgcttatgg
    25381 gacgggaata ccggcgcggg gatacatacc ttatccggcg gtggatctgg ctcagtcaaa
    25441 caacgcctcc gatgccacag gcccgaatcc cttcatgatt aacaaagctt ctgcgccaaa
    25501 agttgatggt gagatcaagg tctcttttgt gaattcgcct ccgggtatgc gggttatgga
    25561 aacgcgatcc agcggttttg atgtcagcca tgatgttggc tatacgcgct ttggcaggta
    25621 atgaaaaatt aatctgttaa tgagtcccac tccggtggga ttttttatgt acggagttta
    25681 tatgacgtgg aaagacagac ttcaggacgc gtcatttcgc ggtgtgccgt ttaaggttga
    25741 agaagaaaat gcgggaaccg gtcgtcgtgt ggaaacgcac gaatacccga accgcgacaa
    25801 accctatacc gaagatctgg gaaaagtcac tttccgcccg tccatcacgg cttatgtggt
    25861 gggagatgac tgctttgacc agcgcgatcg cctgattgac gcgctgaata aacccggtcc
    25921 cggcacgctt gtccatccga catacggtga gctgaaagtc tgtgttgacg gggaggttcg
    25981 ggtcagcaca tcgaagggtg aagggcgtat tgtccgcttt gacctgaagt ttgtcgaagc
    26041 gggagaactc tcttacccca catcaggtgc ggcgacggcg cagacgctga tgtcatcctg
    26101 ttctgcactg gatgactgca tcagtgacag tttcagtggt ttcagtatcg atggcgtggc
    26161 agattttgtg cagaacgacg tcgtcggtaa tgccagcaca atgcttgggt atgtttctga
    26221 tgcgatgaaa gtggtggatt ctgccgtatc ggatgtcgcc aggctgttgc agggggatat
    26281 ctcggtactt ctgccgccgc catcgtcagg caaaaatttc gttgagcagg tgcagaaaat
    26341 gtggcgtacc gggaaacgcc tttatggtaa cgccagcgac ctggtcacca tgatcaaaac
    26401 gctttccggt gtcagcctcg gcagcgatct gcaaccgcgc ggcgtctgga aaacggacag
    26461 taaaaccacc gccacggcga cgcagcagcg taacgtggtt gccagcaccc ttcgtacgac
    26521 cgcaatcagc gaagcggcgt atgccgtcac ccgattgcct gcgccaacaa cttccgcggt
    26581 gatgcagaat gccgcagtgg agcaggcaac aacacctgcg cagagcactg gctggccttc
    26641 cgtcacgcat ccggcactga acaatgcacc ggcggtgaaa aacacggttg acctgccgac
    26701 gtgggaagaa ctgactgaca ttcgcgacac actgaatacg gcaattgata aggagttgtc
    26761 ccgtacaacc agtgatgcgc tgtttctggc gctgcgccgg gtgaaagcag atctgaatgc
    26821 ggatatcaac acgcgccttg aacagtctgc acggatcatt cagcgcacac cggatgaggt
    26881 tttacccgcg ctggtgctgg cggcgacctg gtttgataac gcggcgcgtg acgcggacat
    26941 tatccggcgt aatgccatta cgcatcccgg ctttgtgccg gtgatccctc tgaaggtgcc
    27001 agtgcaatga acgataacgt cacgctacgg gtaaatggcc gggagtggaa tggctggaca
    27061 tcggtgcgca tcggtgccgg tattgaacgg ctggcgcggg atttcagtgt ggagatctcc
    27121 cgccagtggc cgggagatgg gggtatcacc acgcttcagc cgcgcattaa aaacggttca
    27181 aaagtggaag tgctgattgg tgatgagctg gtgatcaccg gctgggtgga ggcgacgccc
    27241 gttcgttacg atgcccgttc ggtcagcacc ggtattgccg gacgcagtct gacggctgac
    27301 ctgattgact gtgcagccga accgacacag tttaacggac gagagctggt acagattgcg
    27361 caggcgcttg ctgcgccttt cggcattgag gtggtgaaca acggtgcgcc gtcgggtgtt
    27421 attcctgacg tccagcccga tcacggtgaa acggtgattg aggtaatcaa caaaatactc
    27481 ggtcagcagc aggcactggc ttacgacgac ccgcacggca ggctggtgat tggtggtatt
    27541 ggctcaacgc gggcacatac tgcgctggta ctcggggaaa acatcctttc ctgcgatacg
    27601 gagaagagta tccgggagcg gttttctgtt taccaggtgg cggggcagcg tgccggaaac
    27661 gacgatgatt tcggtgaggc caccaccacc gcgctgcggg cccgcacaga ggacgcattt
    27721 attgcccgtt accgtccgat gtatatcagg cagacagggc aggccacggg ggcaggctgt
    27781 attgcgcgtg ctgactttga agcccggcaa cgggcggcgc ggacggatga aaccacctat
    27841 gtggtgcagg gctggcgaca gggtaacggt acgctgtggc agcccaacca gcgggtgatt
    27901 gtcttcgatc cggtctgtgg ttttgacaat accgaactgc ttgtctcgga agtcacgttt
    27961 actcaggacc agaatggcac tctgacggaa atccgtgtcg gcccgcctga tgcttatctg
    28021 cctgaacccg aagaccccgg cgcgcggaaa aagaaaaaag ccagagtaca ggaggacccg
    28081 ttctgatgag gacgattgaa gccatgcagc gacaactcct cggcctgatt gggcgggccg
    28141 tggtgaaaag catcagtgcc gccacgaaat gtctgaccgt ggatgtgtcc ctgattgccg
    28201 gtgaacccaa agccggggtt gaacatcttg aaccctacgg ttttaccgca agggcaaaca
    28261 gcggtgcgga agcggtggtg ttgtttccgg atggcgaccg ttctcatgcg gtggttgtta
    28321 cggtgtcaga ccggcgctac cgcctgaaag ggctgcagac gggtgaggtg gctgtctatg
    28381 acgatcaggg gcagtccgtg acgctgaccc gggaggggat cgtggtggac ggtgcaggta
    28441 aaacgatcac gtttcgcaat gcacctgaag cacgttttga aatggacctg gaagtaacag
    28501 gacaggtgaa agacctgtgc gactccggcg gcaccaccat gtcagcaatg cggcttgcct
    28561 ataacggtca tcgtcacaga gagaacggtc agggcagtaa caccgataaa cctgataaag
    28621 ctatggaggc atgatggaac tgtggctgac ggtgaacggt aaacgcacct gcgccagcgc
    28681 accgctggat ccgctgaccc gcgccgtggt gatttccctg tttacctggc ggcgggcgga
    28741 gcctgatgac aacgccgacg tcccgatggg atggtggggg gatacctggc ctgcggtaca
    28801 gaatgaccgt tacgcctcca gactgtggct gcttcagcgc agcaaactga ccaatcagct
    28861 ggtgcagacg gtaagggggt atatccgcga atgcctgcaa tggatgactg atgacggcgt
    28921 ggtgtcccgt attgatctgg atatccgccg caccgggatt aatgaactgg gtaacagtat
    28981 cactctctgg cgtcgtgacg gaccggtaat gatttctttt gatgatctgt ggagtgcgat
    29041 aacgcatggc ggacagtgaa tttcagcgcc cgacgctggc agaaaatatc agtatgctcc
    29101 gtaacgattt attcgccagg ctggacgtca gcgacacgct ccggcgcatg gatgaagacg
    29161 tgcgggcaaa ggtgtatgcg gcggcgctgc atacggttta cgggtacatc gattatctgg
    29221 caatgaacat gctgcctgac ctgtgcgatg agttctggct ggcgcgacat gctgcgatga
    29281 aacggtgtcc gcgcaagggg gccacggctg ccagcgggta tatgcgctgg gaaggtgtca
    29341 gcgatggcct gaaggtgacc gccggaagtg ttattcagcg cgatgacctg gttcagtaca
    29401 cggcaactgc cgatgcaacc agctccggtg gtgtcctgcg cctgccgatc gcctgttcaa
    29461 ctgcaggcgc ggtcggtaac gctgacgacg gtacggcatt aatcctggtc acgccggtga
    29521 atggtctgcc gtcttccggt gtggcagata ccctgacagg tggatttgat actgaagatc
    29581 tggaaacgtg gcgcgcccgc gtcattgagc ggtattactg gacgccgcag ggcggggctg
    29641 acggggacta tgtcgtctgg gctaaagaag tgcccggcat tacccgcgca tgggcatacc
    29701 gtcactggat gggaacgggg actgtcggtg tgatgattgc cagtagtgac ctgattaatc
    29761 ccattccgga agaatcaacg gaaacggcgg caagacaaca tatcgggccg ctggccccgg
    29821 tggcaggctc tgatttgtat gtgttcaggc cggtggcgca tacggtggat tttcatatcc
    29881 gtgtgacgcc ggacacaccg gaaatacggg ctgccatcac cgcggagttg cgttcgttcc
    29941 tgctgcgtga tggttatccg cagggagaac tgaaggtatc gcgtatcagt gaggcgattt
    30001 ccggtgcgaa cggggaatac agccatcagt tgcttgcccc ggctgacaat atctccattg
    30061 caaaaaatga actggcggtt ctggggacga tttcatggac gtgacaaacg atgattacat
    30121 ccgtctgttg tcggcactgt tgccccccgg tccggcgtgg tcagccagag atccggcgat
    30181 tgccggtgcg gcaccgtcat taacccgcgt tcatcagcgt gcggatgtcc tgatgcggga
    30241 gctggatccg cgcaccacca ctgaactgat aaaccgctgg gagcgtctgt gcggtctgcc
    30301 ggatgaatgt attcccgcag ggacacagac ccttcgccag cgtcagcaac gactggatgc
    30361 gaaggttaac ctggcgggcg gcatcaatga ggatttttac cttgcacagc ttgctgccct
    30421 gggcagacca gacgccacca tcacgcgata cgacaaaagc acgttcacct gctcatctgc
    30481 ctgtactgac gcggtgaatg cgccggaatg gcggtattac tggcaggtca acatgccagt
    30541 cgccaccaac accacctgga tgacatgtgg cgatccctgt gattccgcgc tgcgtatctg
    30601 gggtgacacc gttgtcgagt gtgtgcttaa caaactctgc ccgtcgcata cctacgtaat
    30661 ttttaaatat ccggagtaat ccatgcatcg tatagacacg aaaaccgcgc agaaggataa
    30721 gttcggcgcg ggtaagaacg gttttacccg tggtaacccc cagaccggca cacctgctac
    30781 cgatctggat gatgactact ttgacatgtt gcaggaggag ctttgcagcg tggtggaggc
    30841 atccggtgcc agcctggaga aggggcggca tgaccagctg cttaccgcgc ttcgtgcgct
    30901 gctgttaagc cgcaagaatc cgtttggcga tatcaaatcg gatggcacgg tgaaaacggc
    30961 tctcgaaaac cttggtttgg gagaagcagc taaaaggaat gtaggtacag gggcgaatca
    31021 gatacctgat atgagcctgt tcgcgtcaat taataccgta acggctgctg cgcaaaaatt
    31081 tccgtctgga ttaattttac agtgcggtca gttgaatggt gccccgaatg tatcttcaac
    31141 atacgggatg aggttcccga tgacgttctc aagagtcatt gctgtcgtag ttacattgaa
    31201 cgttactggc gcggctgggc agccgactgt atcggcgaca agtgtccaga acactggatt
    31261 taatattact gtgtcgcccg gttcaggata cggttcatct gctgatgcgt attacattgc
    31321 aatgggatat taacaaaatg tcatattttt attctgcatc gacaaacgga ttttatttga
    31381 ctgaatttca cggcaccaat attcctgatg atgcagtgga aatctcggaa tcagagtgga
    31441 aaacactgat taatgcacag agtgtaacaa aaatgattac ctgtggtgag aacggtcatc
    31501 ctgtcattgt tgaccgtcct tctccaacac cagaacaatt agccttaata aatgatgaaa
    31561 agaaatctgc actgatagca gaggcaacga atgtaatagc tccgcttcag gatgcggttg
    31621 atttaggtat ggcaacagat gatgaaacga aactgttact ggcatggaaa aaatatcggg
    31681 tgctgcttat gcgtgttaat gtagtaaaac ccgagtggcc tatgcatcca aataaataga
    31741 cttgttaatc taggataatt gttggaataa tactcatttt tcagtaggtt caggtagcct
    31801 gggtataatg ctcacttcta caatgatttc aacttatttt atttaattga tattacgaaa
    31861 cttaactcat acataaacaa tatgactcaa tactgtccta aatagctgcg cagaaaagtg
    31921 ctccactttc gctcagccct tgctgggcga agcatcagga aggcgttctc agctaggttg
    31981 tgttatatct ggcttgagtt ttgttgccca tacgcaacac attacccatc tgtaaacgac
    32041 aagtgtgaca gaattaggtg cagctattta gactatatcg acgcagtaac gataatagat
    32101 atcgaaactg tactagatat tagctactta tggttaccgg gggaagtctt ttgagcactg
    32161 gtgttatcaa tcggcttcaa ataattcacc aataaattat attgcgatag ctcatcacct
    32221 tcctggttgt ttatagccag tttatcagcg tcaccaccta ctgtttatgc ttttatttct
    32281 ttgattaatg agtatatgat attttaattc atctggtgta tttctttctc atgtgttggc
    32341 cgactgaata tctgttcagt gaaggggtaa ttttatgttt gcacttcgaa cattcccttc
    32401 agttgccaag aaagaaaacc gtaacatttg cgcattaatt ttaacagtat aatctgagca
    32461 taaatatgcg ctatatatct ataaaaaggc gcaattaatt gcgccttttt atcattttat
    32521 caaaaatggc ccaccgactg tcctcgatag ccaatggcca attttatctg atacatatac
    32581 ataaatacag gtatatatga ttgcgcagat aggatttgga atggtcacgc tgaagcctat
    32641 gattgctaaa gccataggga taaataattt ggtgatatac tccattccac atatgttaag
    32701 agtatttctt ccagctctta caataaagtc attcccacaa attatttttg ctataaaaat
    32761 attaaatatt atcaaaccta ccgtggtgat aatagtatta atagcttgaa tataactact
    32821 tgatattatg tctgcattga atattgagat aatcttaaaa agcaaaggtt tctggttaag
    32881 gagttgatac gcactcatta tcgcggtaat tgagaagaca atgaaaccaa
//
LOCUS       DAJTNM010000031        32720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTNM010000031 DAJTNM010000000
VERSION     DAJTNM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.031"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..870)
                     /locus_tag="OO172_001596"
     CDS             complement(<1..870)
                     /locus_tag="OO172_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR5511825.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNREGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLP"
     gene            complement(1048..1959)
                     /gene="gsiD"
                     /locus_tag="OO172_001597"
     CDS             complement(1048..1959)
                     /gene="gsiD"
                     /locus_tag="OO172_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5511826.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1962..2882)
                     /gene="gsiC"
                     /locus_tag="OO172_001598"
     CDS             complement(1962..2882)
                     /gene="gsiC"
                     /locus_tag="OO172_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5511827.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2900..4438)
                     /gene="gsiB"
                     /locus_tag="OO172_001599"
     CDS             complement(2900..4438)
                     /gene="gsiB"
                     /locus_tag="OO172_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5511828.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(4458..6329)
                     /gene="gsiA"
                     /locus_tag="OO172_001600"
     CDS             complement(4458..6329)
                     /gene="gsiA"
                     /locus_tag="OO172_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5511829.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6316..7281)
                     /gene="iaaA"
                     /locus_tag="OO172_001601"
     CDS             complement(6316..7281)
                     /gene="iaaA"
                     /locus_tag="OO172_001601"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5511830.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7485..8720
                     /gene="moeA"
                     /locus_tag="OO172_001602"
     CDS             7485..8720
                     /gene="moeA"
                     /locus_tag="OO172_001602"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5511831.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPISATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8720..9469
                     /gene="moeB"
                     /locus_tag="OO172_001603"
     CDS             8720..9469
                     /gene="moeB"
                     /locus_tag="OO172_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5511832.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9648..10310)
                     /gene="fsa"
                     /locus_tag="OO172_001604"
     CDS             complement(9648..10310)
                     /gene="fsa"
                     /locus_tag="OO172_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5511833.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10441..11340
                     /locus_tag="OO172_001605"
     CDS             10441..11340
                     /locus_tag="OO172_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5511834.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11346..13778
                     /locus_tag="OO172_001606"
     CDS             11346..13778
                     /locus_tag="OO172_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5511835.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13924..14739
                     /gene="ybiV"
                     /locus_tag="OO172_001607"
     CDS             13924..14739
                     /gene="ybiV"
                     /locus_tag="OO172_001607"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5511836.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14891..16156
                     /locus_tag="OO172_001608"
     CDS             14891..16156
                     /locus_tag="OO172_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5511837.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16397..17989)
                     /locus_tag="OO172_001609"
     CDS             complement(16397..17989)
                     /locus_tag="OO172_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5511838.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPECVIDFSGNYEDYLRSKGIE"
     gene            18208..19128
                     /gene="ldtB"
                     /locus_tag="OO172_001610"
     CDS             18208..19128
                     /gene="ldtB"
                     /locus_tag="OO172_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5511839.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19186..20304)
                     /locus_tag="OO172_001611"
     CDS             complement(19186..20304)
                     /locus_tag="OO172_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5511840.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20301..20768)
                     /gene="mntR"
                     /locus_tag="OO172_001612"
     CDS             complement(20301..20768)
                     /gene="mntR"
                     /locus_tag="OO172_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5511841.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20954..21082
                     /gene="mntS"
                     /locus_tag="OO172_001613"
     CDS             20954..21082
                     /gene="mntS"
                     /locus_tag="OO172_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5511842.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21354..22937
                     /gene="opgE"
                     /locus_tag="OO172_001614"
     CDS             21354..22937
                     /gene="opgE"
                     /locus_tag="OO172_001614"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5511843.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22985..23500)
                     /gene="ompX"
                     /locus_tag="OO172_001615"
     CDS             complement(22985..23500)
                     /gene="ompX"
                     /locus_tag="OO172_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5511844.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23553..23618)
                     /locus_tag="OO172_001616"
     CDS             complement(23553..23618)
                     /locus_tag="OO172_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511845.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23853..24740
                     /gene="rhtA"
                     /locus_tag="OO172_001617"
     CDS             23853..24740
                     /gene="rhtA"
                     /locus_tag="OO172_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5511846.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            25039..25542
                     /gene="dps"
                     /locus_tag="OO172_001618"
                     /gene_synonym="pexB"
     CDS             25039..25542
                     /gene="dps"
                     /locus_tag="OO172_001618"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5511847.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25946..26692
                     /gene="glnH"
                     /locus_tag="OO172_001619"
     CDS             25946..26692
                     /gene="glnH"
                     /locus_tag="OO172_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5511848.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            26831..27490
                     /gene="glnP"
                     /locus_tag="OO172_001620"
     CDS             26831..27490
                     /gene="glnP"
                     /locus_tag="OO172_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5511849.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27487..28209
                     /gene="glnQ"
                     /locus_tag="OO172_001621"
     CDS             27487..28209
                     /gene="glnQ"
                     /locus_tag="OO172_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5511850.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            28470..30694
                     /gene="ybiO"
                     /locus_tag="OO172_001622"
                     /pseudo
     CDS             28470..30694
                     /gene="ybiO"
                     /locus_tag="OO172_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(30691..31617)
                     /gene="rlmF"
                     /locus_tag="OO172_001623"
     CDS             complement(30691..31617)
                     /gene="rlmF"
                     /locus_tag="OO172_001623"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5511851.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            31893..32153
                     /locus_tag="OO172_001624"
     CDS             31893..32153
                     /locus_tag="OO172_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5511852.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            32418..>32720
                     /locus_tag="OO172_001625"
     CDS             32418..>32720
                     /locus_tag="OO172_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5511853.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT         8022 a         8737 c         8098 g         7863 t
ORIGIN      
        1 cggtagataa aaaagtttat ctgatgcgtt caataaatta ccgccaggat aataaaaata
       61 gatttcgaac aatgcattat ttggcactgc tgatgtcttt gcaatatact ccgtatcgac
      121 agaatcaata atattataat taccttctct attataaata atcagttttt cacgagcgag
      181 ttgattcttg gcaatgatct catgaccttg tttcaaaaat agaacttcat cattagcgct
      241 gatttgatca agatactcgt tcagataccg ccagtttaaa tcgacatgaa ttttatctac
      301 cgctaacaaa tcgtgatctt tgccgttgat acgtagcgta aaagagatac attttttacc
      361 cggacgatca aagcaattgt atgccggatt ccagctgttg ttagagtcat ctgaagccac
      421 cgaccacggt cgtttcgatg ggttaaaatc ggcaaaaata acccgccctg actcgtcgac
      481 aagcggtcca tttacttgtt cctgatgata aacaacgatt ttatcattcg ccctccgcga
      541 aaagagataa tatttattat catcaagtac cagtcccacc gagtctaaaa atggcatcat
      601 ttccattttc agaatcacgg cgcgttgaat ctcctctgta ttttttaata aatacagtga
      661 gtcctttaat gtcagggccg aacgttcaat atctttaaag aaatagccaa cgtttttctg
      721 aataagttca aagcggctat cggcacgttt taccgcgcgt tccgatacca gttgatagtt
      781 aatcatgaaa aaaagaccaa taaatgacag tgctgccaat aaaaataaaa ttatcagcct
      841 tatctttatc ttttcgtata aactcagcat gttaatacat ttaacaaaat aactatctga
      901 acgctgtgcc gctgcaaaaa tagtcccttg ctgcggcttt ttaaccaaat tacccgattc
      961 tacaaaatca gcagaaagtt acaacacttg cttagggcaa taatttcctg tgaggtgatt
     1021 accctttcaa gcaatattca aacgtaacta tcctttaatt ttcggatcca gcgcatcgcg
     1081 taaaccatcg cccaacaaat tgaacgccag tacggtcaga aaaatagcca gggccggaaa
     1141 aacagcgaca tgcggcgcga taaccatatc cgctcgtgcc tcattgagca ttgctcccca
     1201 ctctggtgtc ggcggctgcg cacctaggcc gagaaatgac aggctggcgg cagagataat
     1261 cgaggtgcca atgcgcatgg tgaaaaacac cacgatagaa gagacggtcc caggcaggat
     1321 atgacgcaat aaaatggtca tatcgctggc accaatactg cgtgctgact caataaaggt
     1381 ttgctgtttc aacaccagcg tgttgccgcg caccaggcgg gcaaacgcgg ggatggaaaa
     1441 aatggcgact gcgataatca cgttagcaat gccgcttccc aacaccgcaa caacagcgat
     1501 cgccagtaaa atacccggga aggcaaacag cacatcgcaa atgcgcatga tcagccgatc
     1561 ccaccagcct tcataatatc cagccaacaa gcccagcaac gtccctatcg ccacaccgat
     1621 aaacacggca aacacgcccg ccgccagcga gatttgcgca ccaaccagga cacggctgaa
     1681 aatgtcgcgc cccagtgaat cgacgccaaa ccagtgctgc aaagaaggtc cgttattcag
     1741 attgtcataa tcaaaataat tttcggcgtc atagggagcg atccagcgtg caaaaatggc
     1801 caccacaatc aataaaataa cgaataatgc ggcggtcatc gccatatgct gacggcgaaa
     1861 tcgtcgccag aattcatgcc acggtgtacg tacctggtca ggtttgacca gtggcatggc
     1921 gtttaacacc gcctgacgtc gccagttaaa tagtcgcatc cttacttgta cctgatagcc
     1981 gggttaatgg cggcgtaaag cacatccacc actaagttga taagaataaa ttccagcgag
     2041 aaaagcagaa tttccgcctg aatcaccggg taatcgcgca tttctacaga gtcaacgagt
     2101 aagcgtccaa gtccgggcca gttgaaaact ttctccacca cgatagaacc tccgagcaaa
     2161 aagccaaact gtaagcccat catggtcact accgggatca tcgcgttgcg tagcccgtgt
     2221 ttgaggacaa cccaggtttc gctcacccct ttcgccctcg cggtacgcat ataatcttcg
     2281 cttaaaacgt cgacaaatga cgcacgggta aagcgcgcca tcacggcggc cactgccgcg
     2341 ccgagcgtca gggagggtaa aatgtagtgc tgccaactgt ctgctcccac agtaggcagc
     2401 cagcccagtt caacggagaa tacctgaatt aaaaacatcc ccagcgcaaa tgccggaaac
     2461 gagatccccg acaccgcaat ggtcatactc aatcgatccg gccaacgatt acgccagacg
     2521 gcggcgataa ttcccgccgc cataccaaat atcaccgccc agaccatact ggttatggtc
     2581 agccacagcg ttggcataaa gcggctggca atctcatcgg caaccggacg acgcgacacc
     2641 atcgacaggc caaaatcccc ctgcacagca ttgctgatat agtgccagaa ctggtgatac
     2701 agcggctgat ccaaccccag ctgctgacgc accagttcta taacctgcgc atcagcttcg
     2761 ggcccggcaa tcaatcgcgc cggatcgccg ggcagcatgt ggacaaataa aaacaccagc
     2821 accgagacga taaacagcgt cggaatcaac cccagtaatc gtttgataac gtaattaagc
     2881 attccactcc ctgcgttgct tattgcaaat ccgcgtcttc aaagctgaag ccgctgtctg
     2941 gcatgatcca aaaaccggtc aggtttttac tgtgtgccga caccagtttt tctaccacca
     3001 gcgggatcca cggcgattct tgccagatga tatcctgcgc cgccttatat aagcgggtct
     3061 tttccgccgg atcattcgtt ttcagtgcct gagccaggaa gtcatccacc tgtttattgc
     3121 tgtaaaacgc ggtattaaac agcgtcggtg gccagttttg cgaggcaaac agcggcgata
     3181 gtgcccagtc ggcttcgccg gttgaagccg accagccagt gtagaacatc cgcacgccgc
     3241 tctctttttg ccctttacct tcaacttctg ccgcccgctg tccggcatcc atcgcggtca
     3301 cctgggcttt aatcccgacc tgcgctaact gctgctgggt aaattgcagc actttctgcg
     3361 cggtgctgtg gttatgtgac gaccacagcg tggtactgaa accgttggga tatcccgcct
     3421 ctttcagtaa ttcgcgcgct ttcactggat cgtaaggcca cggtttataa ctttgcgcgt
     3481 aggcgatact tggcggtacc acaccagtag ctggcgttgc atagcccgca aaggcaactt
     3541 tcaccagcgc cggacggtta atggcgtaat tcagcgcctc acggaccttc gggttatcga
     3601 acggcttttg cgtcacgttc atactgatat aacgctgcat aattgacgga ctggccatca
     3661 actcgatatt tttgtttttc tccagcagtg tggcctgctc gtagggaatg gggaaagcaa
     3721 actgcgcttc accggtttgc agcattgccg cgcgggtgtt gttatccgcc accggacgcc
     3781 aggttatgct gtccagtttg ggcaatcctg gctgccagta acccgcgaat tttttcacct
     3841 tcacaaaatc ggtctgattc caggtatcca gttcatacgg tccggttccc accggatgaa
     3901 aaccaatctc cttgccatat ttttccagcg ctgtcggtga aatcatcgcg gtcgccggat
     3961 gggcaagaat attaataaac gctgagaacg gctgtttgag ggtaatcttt accgttgtcg
     4021 gatcgatcgc ctccgttttg gcaatattct tatacaggtt atagcgttta agatgattcg
     4081 ccggatcgct ggcccggtcc agattcgctt ttaccgccac tgcgttgaaa tcggtgccat
     4141 cctggaattt aattccttcc cgcaatttca cggtgtaagt aatgccgtca tcggaaacgg
     4201 tataactctc cgccagcacg tttttcagtt tcatctcttt atccagaccg aacagcccct
     4261 ggtaaaacga tttcgctacg gcctgagata acgtgtcatt cgcgtcatac ggatcgagcg
     4321 tggtgaaatt cgatcctacc gccaccacca catctttggc agcgaatgca caagatgcca
     4381 tcaacgctgt cgcaatgccc agcgccacta acccactacg gtgtacagct cttgccattt
     4441 tattctccgc ctgaatgtta tctacgcatg aatgcgtatt ctgattgtgg ttgtgcgacg
     4501 taatgccccg gcccgacgca ttgcaacgag acggctgcca cctcttcgcc acgcagatga
     4561 atattgctgg gaagatcgtc cgacagcagt acacgctgcg gtcgttgtcg ggacggttca
     4621 gcgaccggaa ctgccgccag taatttacgc gtataaggat gctgcgggtt ttcgaagacc
     4681 gcgcaccgtg aaccaatttc aacaatttgc ccgagataca tcaccgccac acgatgacta
     4741 atccgctcta ccacagccat atcgtgggag ataaacagat acgcaatgcc gaaatcacgc
     4801 tggagatcga gcaacaagtt gataatctgc ccgcgaatag aaacatccag cgccgaaacg
     4861 gcttcgtcgg caatgatcac ttttggattc aatgccaacg cgcgagcaat gcagatgcgc
     4921 tggcgctgac cgccggaaaa ctcatgcggg taacgccagg catgttcagg taacaggccc
     4981 acgcgctcca gcaaccacgc aacgcgtgca gccgcgtctt tacctggcaa taaaccgtgt
     5041 acacgcagcg gttcgataat cgaatcaccg atggtctgac gtgggtccag cgaagcgtaa
     5101 gggtcctgaa aaataaactg aatatcccgg cgcaatgcct gaagtttgcc aggtgacaag
     5161 gtatcgattc gctgaccgtt aaagataatt tcgccgccct gcgattcgac caggcgcagc
     5221 aacgcccgcc cggtagtgga tttaccgctg ccagactcgc ccaccagcga tagcgtttcg
     5281 ccaggccaga gatcaaaact gactttctca acggcatgca cttcccgcgt tacgcgattc
     5341 aacaaaccgc tgcgcaaagg gaaacgggtg acaagattac gcacccgtaa aacaggttcg
     5401 ccatccacca ccgttttctg ctcgatgggg ggttcctgtt tcgctggatg ttcaagcgat
     5461 atcaatggga aacgtcgggg ataatctaac cctttcatcg caccaagttg cggaacagca
     5521 gctaacagcg cacgggtgta aggatgttgc ggtgcatgaa aaatctgttc gacggtaccc
     5581 gtttccaccg cctcgccctg atacatcacc agtacccgat cggcaatctc tgccaccacg
     5641 cccatatcgt gagtgataaa gataacgccc atcgacatct ctttttgcaa tactttgatt
     5701 aattgcagga tctgcgcctg aatagtgaca tccagcgcgg tggttggctc atcggcaatc
     5761 agcaccgccg ggcggcatga cagcgccatc gcaatcatca ctcgctggcg catcccgcca
     5821 gagagttgat gcgaataacg tgaaagaatg gtttgtgcct ccggaatgcg tacctgatcc
     5881 agcatccgct tcgcctcgac catcgcttct tcacgactgg cgttctgatg cagacgaatt
     5941 gattcggcaa tctgttcacc cacagtaaat actgggttca gcgatgtcat cggctcctga
     6001 aatatcatcg ccatatccgc accgcgcaca tggcgcattt gtgcagcgct ctgctcgcta
     6061 agttcaatca catcacgact gcgccgccgc aacagcattt tatcgcactg tactaaaccg
     6121 cccgcctgtt ccaacagacg catcaatgcc agcgcagtca ctgacttacc ggagccggat
     6181 tcgcctacaa ttgccagcgt ctcaccgcgt tgcagactaa aagagagatt gcggaccgca
     6241 gctattttct gctggtcctg cataaaggca atattaagat tttcaaccgc cagcacatta
     6301 ccggcatcaa gttcatcact gtgtggcaac ggtgtcccct ttttcacggt agataccggt
     6361 ggttggcgta tcgcctgcgt agccccaggc gcgatacatt ccttcggtgt taaacggtag
     6421 cgcgacattc ccttcatggt cgatagcgat taagccaccg ctaccgccaa gcgcagggag
     6481 tttttccatt actacccgct cgcaggcttc cgcgagactt aatccgccgt aatccattaa
     6541 cgcggcgatg tcatatgccg ccagcgcgcg gatgaagact tcgcctgtgc cggtacaaga
     6601 aaccgccaca ctggcgttat tggcgtagca tccggcaccc actaagggac tatcgccaac
     6661 tcgtccgggt aatttattgg tcattccgcc tgtggacgtg gctgccgcca gattgccgtc
     6721 taaatccaac gccacggccc ccacggtgcc cattttctgt ttttcatcca gtggcgcacc
     6781 gctatggtcg aggactgttg ccccttcctc gcgcgctgcc agtagttgtt cataacgcaa
     6841 aggcgtggag aaaatctccg gtgagacgcg ctccatgcca tgagcaaacg caaaattttc
     6901 tgccccttcg ccaatcatca tcacatgcgg gctttgctcc atcaccagcc gggcggcaag
     6961 aaccggatta cgcagatgac taacgcccgc caccgcaccg gctttcaggg tgttaccatc
     7021 catcacacag gcgtccagtt catgggtttc atcacgcgta aagacagcgc caattccggc
     7081 gttaaacagt ggacactctt ccagcagacg caccgcttcc gtcaccacat ccagcgcact
     7141 ttcgccggct tccagcattt tctgcccggt ttcaacaatg gcagacaacg cctcgatgta
     7201 gcgtaattcc tgttgcagac tcatctgcgc gcggctaatt gcacctgcgc caccatgaat
     7261 tgcaatgact gctttgccca tagtattcgt ccattaaggg tggctcgcca cttttgctat
     7321 aaatatcaga atttttatga attactcatt caatttttga gtatagaaag atggatatgg
     7381 catgtaaagg caggagccaa cgctacgccc gaggtggcat acagtctatg gcgtttttct
     7441 gacataatag gcaaattcga ttttgcctcc gcaggagtgt tttcatggaa tttaccaccg
     7501 gattgatgtc gctcgacacc gcgcttaatg agatgctttc tcgcgttacc ccactgacca
     7561 cccaggaaac gctgccactg gttcagtgtt ttggtcgtat tctggcgagc gatgtcgttt
     7621 cgccactgga tgtcccgggg tttgataact ccgctatgga cggctacgcg gtgcgtttag
     7681 ccgatattgc ctccgggcaa ccgctgcccg ttgccggtaa atcctttgcc ggtcagccat
     7741 accatggtga atggcctgcg ggtacctgca ttcgtattat gaccggtgcg ccggtgccgg
     7801 aaggctgcga agcggtggtg atgcaggagc agactgaaca aacggacaat ggcgtgcgtt
     7861 ttactgctga agtgcgtagc gggcaaaata ttcgccgtcg cggtgaagat atctctgcag
     7921 gtgcggttgt tttcccggcg ggaactcgcc tgactaccgc agagctgcca gtaattgctt
     7981 cactggggat tgccgaagtt ccggtgattc gtaaagtgcg tgtagcgctt ttttctaccg
     8041 gtgatgaact ccagttgccc ggtcagccgc tgggcgacgg ccaaatctac gacaccaacc
     8101 gtctcgccgt acacctgatg ttagaacagc tgggttgcga ggtaatcaac ttagggatta
     8161 tccgcgacga tccacatgcc ctgcgcgccg catttattga agccgacagc caggcggatg
     8221 tagtgatcag ttccggcggt gtttcagtgg gtgaagcgga ttacaccaaa acgattcttg
     8281 aagagctggg ggagatcgcc ttctggaagc tggcgattaa accaggtaaa ccgttcgcat
     8341 tcggtaaact cagcaatagc tggttctgcg gcctgccggg caacccgatt tcagcgacgc
     8401 tgaccttcta tcaactggta cagcctttgc tggcaaaact aagcggcaac accgccagcg
     8461 gcctgcccgc gcgccagcgc gtacgcacag cgtcccgcct gaaaaaaacg ccaggacgtc
     8521 ttgatttcca gcgcggcgtg ctgcaacgca acgccgacgg tgaacttgaa gtgacgacca
     8581 ccggacatca gggttcacat atatttagct cctttagtct cggcaactgc tttatcgtgc
     8641 tggaacgcga tcgcggcaat gtggacgtgg gcgaatgggt ggaagtagaa ccgtttaacg
     8701 cgttgttcgg aggcctgtaa tggcggaact cagcgatcag gagatgctgc gctacaaccg
     8761 gcaaatcatt ctgcgcggtt ttgattttga cggtcaggag gcgctaaaag attctcgtgt
     8821 gctggtagtc ggcctcggtg gactcggctg cgcagcttcg cagtatctgg caagcgccgg
     8881 tgtcggcaac ctgacgctgc tcgacttcga cacggtttcg ctctcgaatc tgcaacgcca
     8941 gacactgcac agtgatgcca cggtcgggca accgaaggtg gaatccgccc gtgacgccct
     9001 gacgcggatc aacccacata tcgcgattac gccagtcaat gcactgctag atgacgcaga
     9061 acttgcagca ttgattgctg aacacgatct ggtgctcgac tgtacggata acgttgcggt
     9121 acgtaatcaa ctgaacgcag gctgttttgc cgcgaaggtg ccgctggttt ccggcgcggc
     9181 aattcgtatg gaagggcaaa tcacggtctt cacttatcag gacggcgaac cgtgctatcg
     9241 ctgccttagc cgtttgtttg gcgagaacgc gttaacctgc gtagaagcag gcgtgatggc
     9301 accgttgatc ggcgtaattg gttcgttgca ggcaatggaa gcgatcaaac tgctggcagg
     9361 ttatggcaaa ccggccagcg ggaaaatcgt catgtacgat gcgatgacct gtcagtttcg
     9421 cgaaatgaaa ctgatgcgca atccggggtg tgaggtgtgc gggcagtaat tgccacctcc
     9481 aggcctgata agacgcgaca agcgtcgcat caggcatttg ctccagacgc cagatgcggc
     9541 gtgaacgcct tatctggcct acggttcggg cacagatttc caagcgcggc aggcatcggt
     9601 gcactccgcc acgtcatcag caatttatga ggatgcagaa cgtccggtta aatcgacgtt
     9661 ctgccaaacg ctccctgcca gtcctgctca aacttcgcca cagcggcttc caccgccgga
     9721 tagctaatca tctgttgtgc cacatccagt ggcagagtaa ttgattcaca tccggccagt
     9781 aagcagtcca gcgcctgacg cggggttttg aaactcgctg ccagcacttt cgcctgcggc
     9841 gcatgcattt tcaataactg gtgtaagtcg gtcacagtct gaatgccgct accgccctga
     9901 gcatcaatac gattaacgta aggcgcaaca tattccgcac ctgccagcgc cgacagcagc
     9961 ccttgtgccg cgccatatac cgcggttccc agcgtcggaa tcccttccgc ttttaacatc
    10021 ttaatcgctg ccagcccctc ggctgtcacc ggaactttca ccacgatatc cgcaataata
    10081 gaacgcagct taagcgcgtc attcaccatc ccttcagcag tggtagccat tacctgggca
    10141 aacagacgcc cctgaccgcc catcgcttca tgaagttgcg gaagcacaac atccagcggt
    10201 tttttacccg cggcgataat gcttgggtta gtggtcacac ccgccagcgg aaaaatacgt
    10261 gacagcgcct tcaccgcaac aacgtctgaa gtatccagat acagttccat aaccatcctc
    10321 aaaatgtact ttttgtctga atgtagacta tcacgggaaa actctctgaa cagttgacgc
    10381 acatcaatct aactttcatt cgaaagtatt ttaatgtttg aacgaaacag agaggcaact
    10441 atgattttca atattcagcg ctactcgacc catgatggcc ccggtatccg cacggtcgta
    10501 tttcttaaag gctgttcgct gggctgccgc tggtgtcaga acccggaaag ccgcgcccgt
    10561 acgcaggatc tgctgtatga cgcacgactg tgtctggaag gctgcgagct gtgcgctaag
    10621 gccgcgccgg aagtgattga gcgcgcgctg aatggtttgc ttattcatcg ggaaaagtta
    10681 accccggagc atctgacggc gttaaccgac tgctgtccga cacaggcgtt aaccgtgtgt
    10741 ggtgaagtga aaagcgttga ggagatcatg gcgaccgttc tgcgcgataa accgttttac
    10801 gatcgcagcg gcggcggctt aacgctttcg ggtggtgaac ccttcatgca gccggaaatg
    10861 gcgacggcgc tgctgcaagc ctgccacgag gcaggcattc atactgcggt agaaacctgt
    10921 ctgcatgtgc cgtggaaata tatcgcccct tctctgccct atatcgatct gtttcttgcc
    10981 gatttaaaac acgttgccga cgcgccgttt aaacagtgga ccgacggtaa cgccgccagg
    11041 gtgctggata acctgaaaaa actcgccgca gcgggcaaaa aaatcattat ccgcgtgccg
    11101 ctgattcagg gctttaatgc cgacgaaacc tctgtaaaag ccattaccga ttttgccgcc
    11161 gacgagctgc acgttggcga aattcatttt ctgccctacc acacgctggg catcaacaaa
    11221 tatcacttac ttaatctgcc ctatgacgcc ccggaaaaac cgcttgatgc gccagagctg
    11281 ctcgattttg cccagcagta tgcctgccag aaagggttaa ccgcgacctt acgaggataa
    11341 caaccatgac cacactgaaa ctggacacgc tcagcgaccg cattaaagcg cacaaaaatg
    11401 cgctggtgca tattgtgaaa ccgccagtct gtaccgagcg cgcgcagcac tataccgaga
    11461 tgtatcaaca acatctcgat aagccgatcc cggtacgtcg cgcgctggca ctggcgcatc
    11521 acctggcgaa tcgcaccatc tggatcaaac actatgagtt gatcattggc aaccaggcaa
    11581 gcgaagttcg cgccgcgccg atcttcccgg aatatactgt ctcgtggatc gaaaaagaga
    11641 ttgatgatct ggcagatcgt cccggtgctg gctttgcggt gagcgaagag aacaaacgtg
    11701 ttctgcatga agtgtgcccg tggtggcgcg gtcagacggt acaggatcgc tgctatggca
    11761 tgtttaccga tgagcaaaaa ggtctgctgg cgaccggcat cattaaagcg gaaggcaata
    11821 tgacctccgg cgatgcgcac ctggcggtca atttcccgct gctgctggaa aaagggcttg
    11881 atggtctgcg tgagaaagta gcggaacgtc gctcgcgcat caatctgacg gtgctggaag
    11941 atttgcacgg cgaacagttc ctgaaagcga ttgatatcgt gctggtggca gtcagtgaac
    12001 acattgaacg tttcgctgcc ctggcgcgtg caatggccgc gaccgaaacc cgcgaaagcc
    12061 gtcgcgatga actgctggcg atggcagaaa actgcgatct tatcgcccac cagccgccgc
    12121 agactttctg gcaggcgctg caactgtgtt acttcatcca gttgattttg cagattgaat
    12181 ctaacggtca ctcggtgtcg tttggtcgta tggatcagta tctctacccg tactatcgcc
    12241 gcgacgttga actgaaccag acgctggatc gcgaacacgc catcgaattg ctgcatagct
    12301 gctggttgaa attgctggaa gtgaacaaaa tccgctccgg ctcccactca aaagcctctg
    12361 cgggaagtcc gctgtatcag aacgtcacca ttggcggaca aaatctggtt gatggtcaac
    12421 caatggacgc ggtgaatcca ctctcttacg cgatcctcga atcctgcggt cgcctacgtt
    12481 ccactcagcc taacctcagc gtgcgttacc atgcaggaat gagtaacgat ttcctcgacg
    12541 cctgcgtaca ggtgatccgc tgcggcttcg ggatgccggc attcaacaac gacgaaatcg
    12601 tgatcccgga atttattaaa ctcggtattg aaccgcagga cgcttatgac tacgcagcga
    12661 ttggttgtat agaaaccgcc gtcggtggca aatggggcta tcgctgtacc ggcatgagct
    12721 ttatcaactt cgcccgcgtg atgctggcgg cgctggaagg cgggcgtgat gccaccagcg
    12781 gcaaagtgtt cctgccacaa gaaaaagcgt tgtcggcagg taacttcaac aacttcgatg
    12841 aagtgatggc cgcgtgggat acgcaaatcc gttactacac gcgtaaatca atcgaaatcg
    12901 aatatgtcgt cgacaccatg ctggaagaga acgtgcacga tattctctgc tcggcgctgg
    12961 tggatgactg tattgagcga gcgaaaagta tcaagcaagg cggcgcgaaa tatgactggg
    13021 tttctggcct gcaggtcggt attgccaacc tcggcaacag cctggcggca gtgaagaaac
    13081 tggtgtttga acagggcgcg attggtcagc aacagctggc tgccgcactg gcggatgact
    13141 tcgatggcct gactcatgag caactgcgtc agcggctgat taacggtgcg ccgaagtacg
    13201 gcaacgacga tgataccgtc gatacgctgc tggctcgcgc ttatcagacc tatatcgacg
    13261 aactgaaaca gtaccataac ccacgctacg gtcgtggtcc ggttggcggc aactattacg
    13321 cgggtacgtc gtctatctcc gctaacgtac cgtttggcgc gcaaactatg gcaacgccgg
    13381 acgggcgtaa agcccacacc ccgctggcag aaggcgctag tccggcctct ggtactgacc
    13441 atctcggccc tactgcggtc attggctcag tgggtaaact gcctacggca gcgattctcg
    13501 gcggcgtgtt gctcaaccag aaactgaatc cggcaacgct ggagaacgaa tctgacaagc
    13561 agaaactgat gatcctgctg cgtaccttct ttgaagtgca taaaggctgg catattcagt
    13621 acaacatcgt ttcccgcgaa acgctgctgg aggcgaaaaa acatccggat cagtaccgcg
    13681 atctggtagt gcgtgtcgcg ggttattccg ccttcttcac cgcgctctct ccagacgctc
    13741 aggacgatat catcgcccgt actgaacata tgctgtaatc tcttcggccc ggctgcggcg
    13801 tcgccgggct aaatcattca catcatcaat ttcatcctta ctttcattcg aaatataatt
    13861 tgtgctctgc gtcacattgt tattacactg ttacggtttc attttcgagc caggagcaca
    13921 cctatgagcg taaaagttat cgtcacagat atggacggta cttttcttaa cgacgccaaa
    13981 acgtacaacc aaccacgttt tatggcgcaa tatcaggaac tgaaaaagcg cggcattaag
    14041 ttcgttgtcg ccagcggtaa tcagtattac cagcttattt cgttctttcc tgagctaaag
    14101 gatgagatct cttttgtcgc ggaaaacggc gcactggttt acgaacatgg caagcaactg
    14161 ttccacggcg aactgacccg acatgaatcg cggattgtta ttggcgagtt gctaaaagat
    14221 aagcaactca attttgtcgc ctgcggtctg caaagtgcat atgtcagcga aaatgccccc
    14281 gaagcatttg tcgcactgat ggcaaaacac taccatcgcc tgaaacctgt aaaagattat
    14341 caggagattg acgacgtact gttcaagttt tcgctcaacc tgccggatga acaaatcccg
    14401 ttagtgatcg acaaactaca catagcgctg gatggcatta tgaaacccgt caccagtggt
    14461 tttggcttta tcgacctgat tattcccggt ctacataaag caaacggtat ttcgcggtta
    14521 ctgaaacgct gggatctgtc accgcaaaat gtggtagcga ttggcgacag cggtaacgat
    14581 gcggagatgc tgaaaatggc gcgttattcc tttgcgatgg gcaatgctgc ggaaaacatt
    14641 aaacaaatcg cccgttacgc taccgatgat aataatcatg aaggcgcgct gaatgtgatt
    14701 caggctgtgc tggataacac atcccctttt aacagctgac ctctctaccg gagccatgcg
    14761 ctccggtttt cccttctttt tacatcctgt acatcacatc aaatcattaa acttgcatta
    14821 acttatttat aacttgtatt gtcacatgca aatcagcata gtttcgaatg aaagttaatg
    14881 cgaggtggtt atggcttcta cttttaccag cgacacattg cctgccgatc acaaagcagc
    14941 tatccgtcag atgaagcacg cgctgcgggc gcagcttggc gatgtccagc agatctttaa
    15001 tcagctaagc gatgacattg ccacgcgagt ggctgaaatc aacgcactca aagcacaggg
    15061 cgatgccgtc tggccggtgc tgtcttatgc cgatatcaaa gcaggtcatg ttactgcaga
    15121 gcagcgcgaa cagattaaac gtcgcggttg tgcggtgata aaaggccatt tcccccgcga
    15181 acaagcgcta ggctgggatc agtcgatgct ggactatctg aaccgcaacc gctttgacga
    15241 ggtctacaaa ggccccggcg ataatttctt cgggacgctc agcgcttcac gtcccgagat
    15301 ttaccccatc tactggtcgc aggcgcaaat gcaggcccgc cagagtgaag aaatggcgaa
    15361 tgcgcagtcg tttctcaatc gtctgtggac atttgaaagt gatggaaagc aatggtttaa
    15421 cccggatgtg agcgtcatct accctgaccg tatccgccgc cttccgcccg gaacgacctc
    15481 caaaggtctt ggagcgcata ccgactccgg ggcgctggaa cgctggctgc ttccagcgta
    15541 tcagcacgtt ttcgccaacg tctttaatgg caatctggcg aaatacgatc cctggcatgc
    15601 ggcacatcgt acggaagttg aagagtacac ggtggacaac accaccaaat gttccgtgtt
    15661 tcggacattc cagggctgga ctgcgctctc ggatatgctg cctggtcagg ggttgctgca
    15721 cgtcgtgccc attcctgaag ccatggcgta cgtactgtta cgtccgctgc ttgatgatgt
    15781 gccggaggat gaactgtgcg gcgtagcgcc cggaagagtg ttgccggtat cagagcaatg
    15841 gcatccactg ttaattgagg cgttaaccag cattccaaaa ctcgaggccg gagactccgt
    15901 ctggtggcac tgcgacgtca tccattccgt tgcccccgtt gaaaatcaac agggttgggg
    15961 caacgtgatg tacattcctg cggcaccgat gtgcgagaaa aatcttgcct acgcgcacaa
    16021 ggtgaaggcc gcactggaaa aaggcgcatc gccgggcgac ttcccgcgcg aggactatga
    16081 aacaaactgg gaaggacgct ttacgcttgc cgacctcaac attcacggta agcgagcgtt
    16141 gggcatggat gtttgagagt taatggaagt gaattgatgt gggattaaga aaatcgattc
    16201 gacgttactc agattgctga caagtgcgct ttcttaatgc ctgatgcggc gttaacgcct
    16261 tatccggcct acaaagttct gcagattcaa tatattacag gtactgcgta ggcctgataa
    16321 gcgtagcgca tcaggctatt ttgcatttgt catcagccgt cgtcaccccg acagtatcgg
    16381 ggtgacggat aatcacttac tcgatccctt tactacgcag gtaatcttcg taattaccgc
    16441 taaagtcgat cacgcattcc ggggtgattt ccagaatgcg ggtcgccagg gagcttacga
    16501 actcacggtc gtgtgaaaca aagatcagcg tgccctgata cagttccagc gccatgttca
    16561 gtgactcgat ggattccata tccaggtggt tggtcggttc gtccatgatc agaatgttcg
    16621 gcttctgcat cattaactta ccaaacagca tccgcccttt ttccccaccg gaaagcactt
    16681 tagctggctt tttgatgtcg tcctggctga acagcaaacg accgagaatg ctgcgtaccg
    16741 cctgctcgtc atcgccttcc tgcttccact gactcatcca ttcgaacacg gtcagatcat
    16801 tttcaaactc atattcgtgg tcctgggcgt agtaaccaat gcgcgcgttt tcagaccatt
    16861 ttacggtgcc gctgtccggt tgcagatcgc ccaccagcgt tttcagcagc gttgatttac
    16921 cgacgccgtt ggtacccagt accgcaagtt tttcaccgac ctccagcagc aggttgagat
    16981 ttttaaacag tggaccgtta tcaaaccctt tggtcagacc ttccacttcc agcgcgttac
    17041 ggaacagttt cttatcctgt tcaaaacgga tgaacgggtt ctgacggctg gaggctttca
    17101 cctcttccag tttgatttta tcaatctggc gcgcgcgcga agttgcctgg cgagatttcg
    17161 aggcgttggc gctaaagcgg ctaacgaaag attgcaactc agcaatctgc gctttcttct
    17221 tggcgttatc ggccagcaga cgttcacgcg cctgggtcgc cgccgtcatg tactcatcgt
    17281 agttacccgg ataaacgcgc agctcgccgt aatccagatc cgccatgtgg gtacagacca
    17341 tgttaaggaa gtgacggtcg tgcgagatga tgatcatggt gctgtcacgc tcgttcagca
    17401 cctgttccag ccagcgaatg gtgtcgatgt cgaggttgtt ggtcggttcg tcgagcagga
    17461 gaatatccgg ctcagcaaac agcgcctgcg ccagaagcac acgcagcttc cagccaggag
    17521 caacttcact catcgggccg tagtgctgtt ccactggaat tcccacgcca agcaacagtt
    17581 caccggcgcg agcttccgca gagtaaccgt ccatttcgcc gtatttcact tccagatcgg
    17641 ccactttata gccgtcttct tcactcattt ccggcaaagc atagatgcgg tcgcgctcct
    17701 gcttcacttc ccacaactct ttatgcccca tgatcaccgt atccagcaca gtgaaccctt
    17761 caaaggcaaa ctgatcctga cgcagcttac caatgcgctc gttgggatcg agggaaacgt
    17821 tacccagcgt cggctcaagg tcgccgccga gaatcttcat aaaggtggat ttaccactac
    17881 cgttcgcgcc aatcaggccg taacggttgc cgccgccgaa tttgacggaa atgttttcaa
    17941 acaacggctt actgccgaac tgcatggtga cgttactgga aactaacaca ggcgtatcct
    18001 gaaaagagat atgacaaacc gcgcattatg ccataagtct gaaagaagat cctgctcctc
    18061 gcttaaggtc tacactcaag ccaaaggcaa aaaatgtgat ttcgtacaca tctgatttca
    18121 ctgtgagctg gaatgaactt ataatgcgct tccaatactc taatattctc aacccaatgg
    18181 cctgccaggc acaaaatctc gcttaacatg aatatgaaat tgaaaacatt attcgcagcg
    18241 gccttcgctg ttgtcggctt ttgcagtacc gcctctgcgg taacttatcc tctgccaacc
    18301 gacgggagtc gcctggttgg tcagaatcag gtgatcacca ttcctgaagg taacactcag
    18361 ccgctggagt attttgccgc ggagtaccag atggggcttt ccaatatgat ggaagcgaac
    18421 ccgggtgtgg ataccttcct gccgaaaggc ggtactgtac tgaacattcc gcagcagctg
    18481 atcctgccgg ataccgttca tgaaggcatc gtcattaaca gtgcagagat gcgtctgtat
    18541 tactatccga aagggaccaa caccgttatc gtgctgccga tcggcattgg tcagttaggc
    18601 aaagatacgc ctatcaactg gaccaccaaa gttgagcata agaaagcagg cccgacctgg
    18661 acgccgaccg ccaaaatgca cgcagagtac cgcgctgcgg gcgaaccgct accggctgtc
    18721 gttccggcag gtccggataa cccgatgggt ctgtatgcac tctacatcgg tcgcctgtat
    18781 gctatccatg gcaccaacgc caacttcggt atcggcctgc gtgtaagtca tggttgtgtg
    18841 cgtctacgta acgaagacat caaattcctg ttcgagaaag taccggtcgg tacccgcgta
    18901 cagtttattg atgagccggt aaaagcgacc accgagccag acggcagccg ttatattgaa
    18961 gtccataacc cgctgtctac caccgaagcc cagtttgaag gtcaggaaat cgtgccaatt
    19021 accctgacga agagcgtgca gacagtgacc ggtcagccag atgttgacca ggttgttctt
    19081 gatgaagcga tcaaaaatcg ttccgggatg ccggttcgcc tgaattaatc gtcaaaacgt
    19141 aaaacaaagg cggactcata atccgccttt tttatttgcc agaccttacc aggccgggat
    19201 cataactaac aaaacgtatc ccaccagcgc cgcccacaac agcatcggta ttgaatagag
    19261 atggaaacgc caccagatgc ggcgatcgtt cgccatacgt agcgcaatca aatttgccag
    19321 cgatcctggt aataaaccaa agccccccac gtttaccgcc catgccagta ataaagacgg
    19381 aggtacataa ttgagcagca aaatggtact cggcacgtta ctgatcacct gcgataaacc
    19441 gactgccgtt aaccataacc cgggttcaga taaatgactc acgttaccca acacgccttg
    19501 caacgctgga agctgggtca gtaaatggac gtcgataaac atcgccataa acaccagcag
    19561 caacgtccag tccacactca acaccacgcg acgcgccagc agcacaaagc ccgccgccac
    19621 aatcaccagt ccccacagct cttgtttgaa ctccagcgcc gtcagaaaaa cgatatacag
    19681 ccccagacaa ttccacacca gccgcggttt ccactccggt gtttgcatcc ccgtatggta
    19741 ttgcagcgcc tttccaggga aacaacacca gcacaacagc aggagtgtca gcatcattgc
    19801 gccagccagc ggtgccattt gggcaataaa tccggcaaac gaaagaccag aacgtcccca
    19861 gataagaata ttttgcgggt tgccaattgg cgtcagtagc gaaccagcgt tgactgccag
    19921 cgcctcaaaa ataatcagcc gattaaccgg gatctcacac agtcttttta gcgtgatagt
    19981 cagcggaaca acaataaaca gcgcgacatc gttggtcaga aaggtagaaa gcagcgccgc
    20041 cgccagcacc ataaacatcg ccagccgacg ctccgtagca aagcggcgca ccattttgcg
    20101 ccccagcaca tcaaaataac cgcttaactc cacacctttg gtcagcagca tcaggccgct
    20161 taaggtgatg atggtgtgcc agtcgatagc agcaggccag gatttcggtg caaagggcac
    20221 gaaaaagctt aatccgatac caacaagaat taataactga aaaaaacgat cgccttgcag
    20281 cgtgcgtaaa aaaggcaggc tcatttggca ccgtgtttct gggtaaacaa acgaaaagcg
    20341 tcgagcgtct cttcactaac atggtgctcc atgccttccg cgtcgcgacg ggcgatttcc
    20401 ggactgacgc ccaacaccaa caagaaattt tcgactatct gatgtcgctc gcggctttcc
    20461 tgcgccagct tctctccttc tgccgttaaa aacacgccac gccaggggat catttcaatc
    20521 agccccatgg ttgccagccg cttaagcatt ttagccaccg tcggttgcga aactcccaga
    20581 cgagcagcca tgtccacctg acgagcttcc cccacttccc tgatcaagtc agaaatcagc
    20641 tcaacgtaat catcaataag ctcgcgccga tgcgcctctc tgacctggcg gaacccttca
    20701 acgtgctctt ccacgttcac taactgcgtc actttttttg ctgttggcgt acctgcgcga
    20761 cgactcattg tgcttcctca ttatggtgac gcatacatag cgttctcatt tcgtgcgcac
    20821 attgtaaacc agagttgcga aggtacaaaa aattaacgtt ttagcaatag ctatataata
    20881 tagcctgtgc tatatctgta tgtaatgcaa tcatccctca aggatcgacg ggattagcaa
    20941 gtcaggaggt cttatgaatg agttcaagag gtgtatgcgc gtgtttagtc attctccctt
    21001 taaagtacgg ttaatgctgc tctctatgtt gtgcgatatg gtcaacaaca aaccgcagca
    21061 agataaacct tcagataaat agcggcgtcg cggtacgccg cttcactcct gcttttatgc
    21121 aggcataacg cgttttggtc tgaaaaaaac cactttttgt cggatttgca atccccttcg
    21181 caaaagattt gttcgtcagt agttgacctg aacggcggct cgctctatct tcttgcagcc
    21241 ctgcgtatat tgcggctcgc ggatgcggac ccctttcccc tcttcacgca ctcttgcagg
    21301 tattgaccct tgacgccagg gtaagcacat ggcgtttgtt accatagtgg catatgaatt
    21361 taaccctcaa agaatcgctt gttacccgta gccgggtatt tagcccgtgg actgcgttct
    21421 actttttaca gtcgctatta attaacctcg gcttaggtta ccccttcagt ttgctctaca
    21481 ccgctgcgtt tacggctatt ttgcttttgc tatggcgaac attgcctcgc gtacaaaaag
    21541 ttctggtcgg tgtcagttcg ctggtggcgg cttgttattt cccttttgct caggcctacg
    21601 gcgcgcctaa tttcaataca ttgctggcat tgcactccac caatatggaa gagtcgaccg
    21661 aaatcctgac gatttttccg tggtacagct acctggtcgg cttatttatt tttgcgctcg
    21721 gcgtaatagc aatcaggcga aaaaaagaga atgaaaaagc gcgctggaat accttcgaca
    21781 gcctgtgtct ggtattcagt gtggcgacat tttttgttgc tcccgtgcaa aacctggcct
    21841 ggggtggcgt atttaaactg aaagatactg gctatccggt atttcgtttt gctaaggatg
    21901 tcatcgtcaa taataacgag gtgattgaag agcaagaacg gatggcaaaa ctttccggaa
    21961 tgaaagatac ctggacggtc actgccgtta agccgaagta tcagacctat gtggtagtga
    22021 tcggtgaaag cgcgcgtcgc gatgccctcg gtgcctttgg cggtcactgg gacaataccc
    22081 cgtttgccag cagcgttaac ggtttgatat ttgctgacta cattgccgcc agtggctcca
    22141 cgcagaaatc gcttggctta acgctcaatc gcgtagtcga tggcaaacca cagtttcagg
    22201 ataactttgt caccctggca aatcgcgcgg gcttccagac ctggtggttt tccaaccagg
    22261 gccaaatcgg cgaatacgat actgctatcg ccagcatcgc caaacgtgca gatgaagtgt
    22321 acttcctgaa agaaggtaat tttgaagcag ataaaaacac gaaagacgaa gcgttactgg
    22381 atatgaccgc tcaagtgctg gcgcaagagc actcgcaacc gcagctgatt gttctgcatc
    22441 tgatgggctc gcatccgcag gcctgcgaca ggacacaagg caaatacgaa acctttgtgc
    22501 aatcgaaaga aacgtcgtgc tatctctata ccatgacgca aacggacgat ttactgcgca
    22561 agctgtacga tcagttacgc aacagcggca gcagcttctc tctggtttac ttttctgacc
    22621 acggtctggc ctttaaagag cgcggcaaag acgtgcaata ccttgcccat gatgataaat
    22681 atcagcaaaa tttccaggtg ccttttatgg tcatttccag cggcgataaa gcgcatcggg
    22741 tgattaaagc ccgccgctca gccaatgact tcttaggctt tttctcgcag tggacgggaa
    22801 ttaaagcgaa ggaaataaat atcaaatacc cgtttatatc tgagaagaaa gccgggccga
    22861 tatacatcac caacttccag ttacagaagg tggattacaa ccatctcgga accgatattt
    22921 tcgacccgaa accttaaaaa caaaaatccg ccccgagagg cggattttta tatcaccaaa
    22981 gtgattagaa gcggtaacca acaccggcaa tccaggtgcc tacgtcaacg ctacgaatac
    23041 ggctctgctc gtaagagaag tccagagcaa cgttttccat cgggttgaac tgcagaccag
    23101 caccgtagga gaaaccgtag tcgctggtgt cgtgtttgta ggtcgggtat tcagtggtct
    23161 ggaatttacc ataacccaca cccactacac cgtagatgct tgcccagtcg ttaatgcggt
    23221 aagccggacc agcagtgatg ccgtagtact ggtttttgtt gtagtcacca gagcttgcag
    23281 tacggctttt ctcggtgtaa gtgaaagaac cgatcacacc cagcgggctg ttgtcttctt
    23341 catagcggta tttcaggttg aaaccgccca ttttgttcat ttggccctga gcgtcgctct
    23401 gtgcgtaacc gccagttacg gtagaagtcg cagctacgga agtgcctgcg gtgaaagcca
    23461 gaactgcggc cagtgctgaa agacatgcaa tttttttcat aaccacctca aatgtgattc
    23521 aaataagtcc taagttttaa atatatcaaa aattaatggg aaactcttcg cgatttgtga
    23581 tgtctaacgg gccatttcat gtaacagaac gtttccatac accgctatcc atctaaattt
    23641 aaatcacttt ttcagagaac tgcgtaagta ttacgcatgt tttccctgtc attcatccag
    23701 attattccta atcaccagac taatgattcc atcaatcctg gcgcatttta gtcaaaacgg
    23761 gggaaaattt tttcaacaaa tgctcaacca gcattgggta tatccagtac actccacgct
    23821 ttacttaagt ctagatattt gtgggagaaa ggatgcctgg ttcattacgt aaaatgccgg
    23881 tctggttacc aatagtcata ttactcgttg ccatggcgtc tattcagggt ggagcctcgt
    23941 tagctaagtc actttttcct ctggtgggcg caccgggtgt cactgcgctg cgtctggcat
    24001 taggaacgct gatcctcatc gcgttcttta agccatggcg actgcgcttt gccaaagagc
    24061 aacggttacc gctgttgttt tacggcgttt cgctgggtgg gatgaattat cttttttatc
    24121 tttctattca gacagtaccg ctgggtattg cggtggcgct ggagttcacc ggaccactgg
    24181 cggtggcgct gttctcttct cgtcgcccgg tagatttcgt ctgggttgtg ctggcggttc
    24241 ttggtctgtg gttcctgcta ccgctggggc aagacgtttc ccatgtcgat ttaaccggct
    24301 gtgcgctggc actgggggcc ggggcttgtt gggctattta cattttaagt gggcaacgcg
    24361 caggagcgga acatggccct gcgacggtgg caattggttc gttgattgca gcgttaattt
    24421 tcgtgccaat tggagcgctt caggctggtg aagcactctg gcactggtcg gttattccat
    24481 tgggtctggc tgtcgctatt ctctcgaccg ctctgcctta ttcgctggaa atgattgccc
    24541 tcacccgttt gccaacacgg acatttggta cgctgatgag catggaaccg gcgctggctg
    24601 ccgtttccgg gatgattttc ctcggagaaa cactgacacc catacagctg ctggcgctcg
    24661 gcgctatcat cgccgcttca atggggtcta cgctgacagt acgcaaagag agcaaaataa
    24721 aagaattaga cattaattaa atttacattt ctgtatggtt atgcataacc atgcagaatt
    24781 tctcgctact tttcctctac accgtcttta tatatcgaat tatgcaaaag catatttatt
    24841 ccgaaaattc ctggcgagca gataaataag aattgttctt atcaatatat ctaactcatt
    24901 gaatctttat tagttttgtt tttcacgctt gttaccacta ttagtgtgat aggaacagcc
    24961 agaatagcgg aacacatagc cggtgctata cttagtctcg ttaattactg ggacataaca
    25021 tcaagaggat atgaaattat gagtaccgct aaattagtta aatcaaaagc gaccaatctg
    25081 ctttataccc gcaacgatgt ctccgacagc gagaaaaaag caacagtaga gttgctgaat
    25141 cgccaggtta tccagtttat tgatctttct ttgattacca aacaagcgca ctggaacatg
    25201 cgcggcgcta acttcattgc cgtacatgaa atgctggatg gcttccgcac cgcactgatc
    25261 gatcatctgg ataccatggc agaacgtgcc gtgcagctgg gcggtgtagc tctggggacc
    25321 actcaagtta tcaacagcaa aaccccgctg aaaagttacc cgctggacat ccacaacgtt
    25381 caggatcacc tgaaagaact ggctgaccgt tacgcaatcg tcgctaatga cgtacgcaaa
    25441 gcgattggcg aagcgaaaga tgacgacacc gcagatatcc tgaccgccgc gtctcgcgac
    25501 ctggataaat tcctgtggtt tatcgagtct aacatcgaat aaatccatcg ctgatggtgc
    25561 agaactttag tacccgataa aagcggcttc ctgacaggag gccgttttgt tttgcagccc
    25621 acctcaacgc acttatttag tgcatccatc tgctatcccc agctgattaa gtaaattttt
    25681 tgtatccaca tcatcacaca accgttacat aaagattgtt ttttcatcag gttttacgct
    25741 aaataatcac tgtgttgagt gcacaatttt agcgcaccag attggtgccc cagaatggtg
    25801 catcttcagg gtattgctct ataaatcgtg catcacgttt ttgccgcatc tcgaaaaatc
    25861 aatgagttgc aaaactggca cgattttttc atatatgtga atgtcacgca ggggatcgtc
    25921 ccgtggatag aaaaaaggaa atgctatgaa gtctgtatta aaagtttcac tggctgcact
    25981 gaccctggct tttgcggttt cttctcatgc cgcggataaa aaattagttg tcgcgacgga
    26041 taccgccttc gttccgtttg aatttaaaca gggcgataaa tatgtgggct ttgacgttga
    26101 tctgtgggct gccatcgcta aagagctgaa gctggattac gaactgaagc cgatggattt
    26161 cagtgggatc attccggcac tgcaaaccaa aaacgtcgat ctggcgctgg cgggcattac
    26221 catcaccgac gagcgtaaaa aagcgatcga tttctctgac ggctactaca aaagcggcct
    26281 gttagtgatg gtgaaagcta acaataacga tgtgaaaagc gtgaaagatc tcgacgggaa
    26341 agtggttgct gtgaagagcg gtactggctc cgttgattac gcgaaagcaa acatcaaaac
    26401 taaagatctg cgtcagttcc cgaacatcga taacgcctat atggaactgg gcaccaaccg
    26461 cgcagacgcc gttctgcacg atacgccaaa cattctgtac ttcatcaaaa ccgccggtaa
    26521 cggtcagttc aaagcggtag gtgactctct ggaagcgcag caatacggta ttgcgttccc
    26581 gaaaggtagc gacgagctgc gtgacaaagt caacggcgcg ttgaaaaccc tgcgcgagaa
    26641 cggaacttac aacgaagtct acaaaaaatg gttcagtact gaaccgaaat aataacgcta
    26701 cacctgtaaa gcgcactggc agttccctct cccccctggg gagaggatta gggtgagggg
    26761 cgcaaacccg ctccggggcc attaattacc ctgaatttga ttatttacac cacggtaaca
    26821 ggaacaacat atgcagtttg actggagtgc catctggcct gccattccgc ttctgattga
    26881 aggtgccaaa atgaccctgt ggatttcggt cctcggtctg gctggcggtc tggtaatcgg
    26941 attgctggca ggttttgcac gcaccttcgg tggttggata gccaaccacg tcgcgctggt
    27001 ctttattgaa gtgatccgcg gcacacctat cgtcgtccag gtgatgttta tctatttcgc
    27061 cctgccgatg gcgtttaacg acttacgcat cgacccattt actgcggcgg tggtcaccat
    27121 catgatcaac tccggcgcgt acattgcgga aatcacgcgt ggtgcggtgc tgtctatcca
    27181 caaaggtttt cgtgaagcag gactggcgct cggtctttca cgttgggaaa ccattcgcta
    27241 cgtcatttta ccgctggcac tgcgtcgtat gctgccgccg ctgggtaacc agtggatcat
    27301 cagcattaaa gacacctcac tgtttattgt tatcggcgtg gcggaactga cccgtcaggg
    27361 gcaagaaatt attgccggta acttccgcgc ccttgagatc tggagcgccg tggcggtgtt
    27421 ctatctgatt attaccctgg tgctgagctt tattctgcgt cgtctggaaa gaaggatgaa
    27481 aatcctgtga ttgaatttaa aaacgtctcc aagcactttg gcccaaccca ggtgctgcac
    27541 aatatcgatt tgaacattgc ccagggcgaa gtcgtggtga ttatcgggcc gtccggttcc
    27601 ggtaaatcga ccctgctgcg ctgcatcaac aaactggaag aaatcacctc cggcgatctg
    27661 attgtcgatg gcctgaaggt taacgatccg aaagttgacg agcgcctgat tcgccaggaa
    27721 gcaggtatgg tgttccagca gttttacctc ttcccgcatc tgacggcgct gggaaacgtc
    27781 atgtttggtc cgctacgcgt gcgtggcgcg aacaaagaag aggcggaaaa actggcgcgt
    27841 gagctgctgg cgaaagtcgg tctggcagaa cgtgcacatc actacccttc cgaactttct
    27901 ggtggtcaac agcagcgtgt ggcgattgcc cgcgcgctgg cggtgaagcc gaaaatgatg
    27961 ctgtttgatg aaccgacttc cgctcttgac ccggaactgc gccatgaagt gctgaaggtt
    28021 atgcaggatc tggctgaaga agggatgacg atggtgatcg tgacccacga aatcggtttt
    28081 gccgagaaag tagcttcgcg gctgatcttt atcgacaaag gccggattgc ggaagatggc
    28141 aatccgcagg tgttgatcaa gaacccgccg agccagcgct tgcaggaatt tttgcagcac
    28201 gtctcttaat aagacacatt gcctgatgcg ctacgcttat caggcctaca ggatatctgg
    28261 caacttatta aaattgcatg aacttgtagg acggataagg cgttcacgcc gcatccggca
    28321 aaaaagcccg cacgttgtca gcaacctgct taatatccct tcctcccttt cacccgaaag
    28381 ggaggcacac cagattcctc tcatttaaaa tcgcccctcc cccagcatct atacttatct
    28441 ttttgctctg ttttctcact ggaggagtca tgcggtggat cctgttcatc ctcttctgcc
    28501 tgctgggcgc acctgcccac gcggtatcca tacccggcgt tacaaccaca acgacaacgg
    28561 actcaacgac tgaaccggcc ccggaaccgg atatcgaaca aaaaaagcgg cctatggcgc
    28621 actggcggat gtgctggata acgacacctc gcgtaaagag ttgatcgacc agttgcgtac
    28681 tgtcgccgcg acgccccctg cggaaccggt cccgaagatc gtgccgccga cgctggttga
    28741 agagcaaacc gtgctgcaaa aggtcaccga agtcagccgc cattatggtg aagccctttc
    28801 cgcccgcttc gggcaacttt atcgcaatat caccggctcc ccgcataagc catttaatcc
    28861 acaaaccttc agcaatgcgc tgagccattt ttcaatgtta gcggtattag tgtttggttt
    28921 ttactggctg attcgcctgt gcgcactgcc gctatatcgc aaaatgggtc agtgggcgag
    28981 gcaaaaaaat cgtgagcgca gcaactggtt gcagcttccg gcgatgatta ttggggcgtt
    29041 tattatcgac ctgctgttac tggccctgac attatttgtc ggccaggtat taagcgacaa
    29101 cctgaatgca ggtagtcgca ccatcgcttt ccaacaaagt ttgtttctca acgcctttgc
    29161 cctgattgaa tttttcaaag ccgtactacg cctgattttt tgcccaaacg tggcggagct
    29221 gcgcccgttc acgattcagg acgagagcgc ccgttactgg agtcgccgcc tgagctggtt
    29281 aagcagcctg attggctatg gcctgattgt ggccgtgccg attatctcta atcaggtgaa
    29341 tgtacagata ggtgcgctgg cgaacgtcat cattatgctg tgcatgaccg tctgggcgtt
    29401 gtacctgatc ttccgaaata aaaaagagat tacccagcat ttgctcaact ttgcggagca
    29461 ttcgctggct tttttcagcc tgtttatccg cgcctttgcg ctggtgtggc actggctggc
    29521 aagcgcctat tttatcgtgc tgtttttctt ttcgttgttc gatccgggca acagtctgaa
    29581 atttatgatg ggtgcaacgg tgcgcagcct ggcgattatt ggtatcgcag cgtttgtttc
    29641 cggtatgttt tcccgctggc tggcgaaaac catcaccctc tcgccacata ctcagcgtaa
    29701 ctatccggag ctacaaaaac ggctgaatgg ctggctttcg gcggcgctga aaacggcgcg
    29761 tattctgacc gtctgcgtgg cagtaatgtt gctgttgagc gcatggggat tgttcgattt
    29821 ctggaactgg ctgcaaaacg gcgcggggca gaaaaccgta gatatcctga tccgcatcgc
    29881 actcattctt ttcttctcgg cggttggctg gacggtgctc gccagtttga tcgaaaaccg
    29941 gctggcttcg gatattcatg gccgcccgct acccagcgcc cgtacgcgta ccctgctgac
    30001 gctgttccgt aacgcgctgg cggtgattat cagtaccatc accatcatga ttgtgttgtc
    30061 ggaaatcggc gtcaatatcg cgccattgct ggcaggtgcc ggggcattag gtctggctat
    30121 ctcgtttggt tcgcaaacgc tggtaaaaga tattatcacc ggggtattta ttcagtttga
    30181 aaacggcatg aacactggag atttggtgac tatcgggccg ttgaccggca cagtggaacg
    30241 gatgtcgatt cgctccgtgg gcgtgcgcca ggataccggg gcgtatcaca tcattccgtg
    30301 gtcttcgata accacctttg ctaacttcgt ccgcggtatt ggttcggtag tggcaaacta
    30361 cgatgttgat cgccatgaag atgctgataa agccaatcag gcactgaaag atgcggtaac
    30421 ggaattgatg gaaaacgaag aaattcgcgg gctgattatt ggtgaaccga actttgccgg
    30481 gattgtcggc ttaagcaata ccgcgtttac actgcgtgtt tcgttcacca cgctgccact
    30541 caaacagtgg acggtacgct ttgccctcga cagccaggtg aaaaaacatt tcgacctggc
    30601 gggcgttcgc gcgccagtgc agacttatca ggtgctgcct gctccgggcg cgaccccggc
    30661 tgaaccgctg ccgccggggg agccaacgct ttaacgttgg cgattaacaa aacggcggcg
    30721 ctgctcgtcg ttcataaagg tccaggcaat aaagcgactc tgcttttgcc cctgggccat
    30781 ctcttttttt accaccttca ccgcgcccac gtcggtcaga gcacgataca acggcggtaa
    30841 gttttcacca cgagatacca gcgaggtaaa ccacatcacc tgcttcgcga agcctttgct
    30901 ctcttcaatc atctttttga taaagttgac ttcaccgcct tcacaccaca actcctgttg
    30961 ctgaccacca aagttcagtg catcgtcttt gttcagcccc aggttacggc gtttacgctc
    31021 actacctgcc cgcgccgcag cggcggaatc gtggaatggc gggttacaca aggtcgcgtc
    31081 gtattgctcg tttttgtgga tgatgccgtt aaaaatcgcc ccactctctt tttgccgacg
    31141 cagacgaatg gcgcggttaa gccccggatt agcactgata atcgcctgcg cactgcttaa
    31201 cgcctggctg ctggtttcac taccggtaaa acgccagcca tattcatgta cgccaatcag
    31261 cggataaata cagttcgcac caacgccgat atccagaatg ctggcattcg ctggaattgt
    31321 tccgcttgct tcagccagta aatcggcaag gtgatgaata taatccgccc ggcccggtac
    31381 tggtgggcag agaaaaccgt cggggatatc ccagttcgct acggcgtaaa aatgggccag
    31441 caacgcctta ttgagcgcct tcaccgccag cggattggca aagtctacgc tttgctcccc
    31501 tgcgggtgta agcgtgagga attgcctgag ttcaggattg acctgacaaa gcgtggcgag
    31561 atcgtagcgg ctgtgatgac ggttgcgcgg atgcaacccc ggtttctggg cggacatagc
    31621 attctccttt aagcagcggc gtaagatacc cgcataacgc gacagggtaa atatctggct
    31681 gcgtttatca ttccccaaaa ccttaacatg gctatggttt atggcaattc atcataaaaa
    31741 tcagcattat gcatgtcatt gatttgtatt attttgtaaa aatatatcgt tcaaaaaata
    31801 agcatatctc tgtcaatttt ttgcgtcatt tgcatcgcgc aggcgaccta cacttatcag
    31861 gcactacccg ttgtatttat gaggatgtca ttatgaaaaa gtgcctcaca ctactgattg
    31921 ccaccgtcct gagcggaatc tctctcacgg cttatgccgc gcaaccgatg agtaacctgg
    31981 acagcggtca gctacggccc gccggtaccg tttcggcaac tggcgcatca aacctaagcg
    32041 atctggagga taaactggca gaaaaagcgc gcgaacaagg cgcgaaaggt tatgtcatta
    32101 attccgcagg cggaaatgac cagatgttcg gtactgcaac catctacaaa taaccccacc
    32161 aacgtaactg cattcgcctc tggatgcagt tttcgcctgt tttgtaatca aaaaacatca
    32221 aatatgaacg caatgtaaat aaatgtattt ctttttcgcg caatgggtga tagaaaatcg
    32281 cgtcaagtga taatgcttat caaaattatt atcacttgca caaacactat cacgggatta
    32341 acagtggcat cgcatccgca gagatgcttt ctcgtggcag tgaaaatttc aacatataag
    32401 aaaaagtcac ctgcaaaatg gaaaacaatc gcaatttccc tgccagacaa tttcattcgc
    32461 tcacgttctt tgccggtctt tgtattggta ttacgcctgt ggcacaggca ctcgccgccg
    32521 aagggcaagc taacgcggat gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg
    32581 cgccacaaca atctgccgat ccgaaattct cgcgtccggt agcggatact acccgcacga
    32641 tgacggtgat ttctgaacaa gtgattaaag atcagggcgc aaccaacctt accgacgcgc
    32701 tcaaaaacgt ccccggcgtg
//
LOCUS       DAJTNM010000032        32176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTNM010000032 DAJTNM010000000
VERSION     DAJTNM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.032"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(75..611)
                     /gene="ssb1"
                     /locus_tag="OO172_001626"
     CDS             complement(75..611)
                     /gene="ssb1"
                     /locus_tag="OO172_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5511854.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNKFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            865..3687
                     /gene="uvrA"
                     /locus_tag="OO172_001627"
     CDS             865..3687
                     /gene="uvrA"
                     /locus_tag="OO172_001627"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5511855.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3722..4078)
                     /locus_tag="OO172_001628"
     CDS             complement(3722..4078)
                     /locus_tag="OO172_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5511856.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4082..4498)
                     /locus_tag="OO172_001629"
     CDS             complement(4082..4498)
                     /locus_tag="OO172_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5511857.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4609..5322)
                     /gene="aphA"
                     /locus_tag="OO172_001630"
     CDS             complement(4609..5322)
                     /gene="aphA"
                     /locus_tag="OO172_001630"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5511858.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5724..5946
                     /locus_tag="OO172_001631"
                     /pseudo
     CDS             5724..5946
                     /locus_tag="OO172_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6450..7643)
                     /gene="tyrB"
                     /locus_tag="OO172_001632"
     CDS             complement(6450..7643)
                     /gene="tyrB"
                     /locus_tag="OO172_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5511859.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7896..8975)
                     /gene="alr"
                     /locus_tag="OO172_001633"
     CDS             complement(7896..8975)
                     /gene="alr"
                     /locus_tag="OO172_001633"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5511860.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9028..10443)
                     /gene="dnaB"
                     /locus_tag="OO172_001634"
     CDS             complement(9028..10443)
                     /gene="dnaB"
                     /locus_tag="OO172_001634"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5511861.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10526..11509
                     /locus_tag="OO172_001635"
     CDS             10526..11509
                     /locus_tag="OO172_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5511862.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQPAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11675..11917)
                     /gene="pspG"
                     /locus_tag="OO172_001636"
     CDS             complement(11675..11917)
                     /gene="pspG"
                     /locus_tag="OO172_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5511863.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12051..13088)
                     /gene="dusA"
                     /locus_tag="OO172_001637"
     CDS             complement(12051..13088)
                     /gene="dusA"
                     /locus_tag="OO172_001637"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5511864.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13451..14436)
                     /locus_tag="OO172_001638"
                     /pseudo
     CDS             complement(13451..14436)
                     /locus_tag="OO172_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14754..15269
                     /gene="zur"
                     /locus_tag="OO172_001639"
     CDS             14754..15269
                     /gene="zur"
                     /locus_tag="OO172_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5511865.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15311..15520)
                     /locus_tag="OO172_001640"
     CDS             complement(15311..15520)
                     /locus_tag="OO172_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5511866.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15636..16961)
                     /gene="dinF"
                     /locus_tag="OO172_001641"
     CDS             complement(15636..16961)
                     /gene="dinF"
                     /locus_tag="OO172_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5511867.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17034..17642)
                     /gene="lexA"
                     /locus_tag="OO172_001642"
     CDS             complement(17034..17642)
                     /gene="lexA"
                     /locus_tag="OO172_001642"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5511868.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17752..18120)
                     /gene="dgkA"
                     /locus_tag="OO172_001643"
     CDS             complement(17752..18120)
                     /gene="dgkA"
                     /locus_tag="OO172_001643"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5511869.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18291..20714
                     /gene="plsB"
                     /locus_tag="OO172_001644"
     CDS             18291..20714
                     /gene="plsB"
                     /locus_tag="OO172_001644"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5511870.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20869..21741)
                     /gene="ubiA"
                     /locus_tag="OO172_001645"
     CDS             complement(20869..21741)
                     /gene="ubiA"
                     /locus_tag="OO172_001645"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5511871.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21754..22251)
                     /gene="ubiC"
                     /locus_tag="OO172_001646"
     CDS             complement(21754..22251)
                     /gene="ubiC"
                     /locus_tag="OO172_001646"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5511872.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22474..24054)
                     /locus_tag="OO172_001647"
                     /pseudo
     CDS             complement(22474..24054)
                     /locus_tag="OO172_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24283..25203)
                     /gene="malM"
                     /locus_tag="OO172_001648"
     CDS             complement(24283..25203)
                     /gene="malM"
                     /locus_tag="OO172_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5511873.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLSVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25355..26695)
                     /gene="lamB"
                     /locus_tag="OO172_001649"
     CDS             complement(25355..26695)
                     /gene="lamB"
                     /locus_tag="OO172_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5511874.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26767..27882)
                     /gene="malK"
                     /locus_tag="OO172_001650"
     CDS             complement(26767..27882)
                     /gene="malK"
                     /locus_tag="OO172_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5511875.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28247..29437
                     /gene="malE"
                     /locus_tag="OO172_001651"
     CDS             28247..29437
                     /gene="malE"
                     /locus_tag="OO172_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5511876.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29591..31135
                     /gene="malF"
                     /locus_tag="OO172_001652"
     CDS             29591..31135
                     /gene="malF"
                     /locus_tag="OO172_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5511877.1"
                     /translation="MDVIKKKHWWQSDALKWSVPGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITTILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31150..32040
                     /gene="malG"
                     /locus_tag="OO172_001653"
     CDS             31150..32040
                     /gene="malG"
                     /locus_tag="OO172_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5511878.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8183 a         8263 c         7784 g         7946 t
ORIGIN      
        1 gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat ataacctatt
       61 gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg ctcgttagac
      121 ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa cttattgcca
      181 ccctgcggct gctgaggctg accccaaccg ccctgcggct gaccaccacc gatattgcca
      241 cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc gccaacgttc
      301 accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt acgggtacgc
      361 agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctcgc cacttctgcc
      421 agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc gccggtcgct
      481 ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc accatttggc
      541 atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt gtttacgcct
      601 ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta aacgcgcgat
      661 tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga aacaaacctg
      721 gccagacatt gttacacaac actccggata atgcattcca atactgtata ttcattcagg
      781 tcaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca ccgggcaaaa
      841 aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc ccgcacccat
      901 aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt gaccgggctt
      961 tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg gcagcgccgt
     1021 tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga aaagccggac
     1081 gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc gacgtctcat
     1141 aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg tttgttgttc
     1201 gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc gcaaaccgtc
     1261 agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat gctgctcgcg
     1321 ccaatcatta aagagcgcaa aggcgaacac accaaaacgc tggagaacct ggcaagccag
     1381 ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc gccgaaactg
     1441 gaactgcaaa agaaacatac cattgaagtg gtggttgatc gcttcaaggt gcgtgacgat
     1501 cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg aactttccgg tggtacagcg
     1561 gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc caacttcgcc
     1621 tgcccaattt gcggctacag tatgcgtgaa ctggagccgc gactgttttc gtttaacaac
     1681 ccggcaggtg cctgcccgac ctgtgacggc cttggcgtac agcaatattt cgatcctgac
     1741 cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg ctgggatcgc
     1801 cgcaacttct attacttcca gatgctgaaa tcgctggcag atcactataa gttcgacgtc
     1861 gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta cggttctggc
     1921 aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat ccgtcgtcat
     1981 ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacaga atccagtgcg
     2041 gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg cgaagggacg
     2101 cgtctgcgtc gggaagcgcg ccacgtttat gtcgagaata cgccgctgcc tgctatctcc
     2161 gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactcgc tggtcagcgg
     2221 gcgaagattg cggaaaaaat tcttaaagag atcggcgatc gcctgaaatt cctcgtaaac
     2281 gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg cggtgaagcc
     2341 cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat gtacgtgctg
     2401 gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg tacgcttatc
     2461 catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga cgcaattcgc
     2521 gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg tgaagtggtc
     2581 gcagaaggtc cgctggaagc gattatggcg gtgcctgaat cgttgaccgg gcagtacatg
     2641 agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc agaaaaagtg
     2701 ctgaagctga caggcgcacg cggcaacaac ctgaaggacg tgacgctcac gctgccagtc
     2761 ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct gattaacgac
     2821 acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga accggcaccg
     2881 tatcgcgata ttcagggact ggagcatttc gataaagtga tcgatatcga ccaaagccca
     2941 attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac gcctgtgcgc
     3001 gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg acgtttcagc
     3061 tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat caaagtggag
     3121 atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa acgctataac
     3181 cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct ggatatgacc
     3241 atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa gctgcaaacg
     3301 ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac actttctggt
     3361 ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac cgggcagacg
     3421 ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca gcaactcctc
     3481 gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga gcacaatctc
     3541 gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg cagtggcggc
     3601 ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc ttcgcatacg
     3661 gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg gccttttcct
     3721 ttcagagttg taccagcaat ttacgctttt cttccggtag taaattcact gcctgctgat
     3781 aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg agatacacgg
     3841 tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc tgctgacgta
     3901 gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt tctacttctt
     3961 tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag tcgttatgca
     4021 cgctctgttc tgcgcctggt tttgccatgc aatattgtag caactccgaa atggtcattt
     4081 tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg cgatgttccc
     4141 ccagccagat gccttgccaa gtgccggtct gaatacgccc tttatgtacc ggcaatacaa
     4201 gcgatgttcc cagcattgag gatttgatat gagaaggcat atcgtctgct ccctcatagt
     4261 catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacactcc atgtcgtggc
     4321 gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt tgcagcaaca
     4381 gatgcagtaa gccgatgtta acgcgcggca tatcagccag ctgattcaga atttcatccg
     4441 ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga taccacatgt
     4501 gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa ggataaaggt
     4561 gtgattaaaa aaacagcatt cgggagagca tcactctctc ccgctctgtc agtattctga
     4621 attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt aggtagagtt
     4681 ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg taatatcgtt
     4741 atcggaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgcg attttgtatt
     4801 ttgccctggt ttatcgcccg caaagattac cggattcatg ttggtggcag gaatatgaaa
     4861 attatccgcc agcgtttttg aaaccgtttc tgttttcgtc gggctacgac cagtcacaaa
     4921 gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga cctcttttgg
     4981 aatgctgaat tcatcccagc cattattcat tttttcccag aacacaggat ttttcagata
     5041 atcttcgctt tctggcgaga aggttttttt gccgcgccag aaccccggac tggaaaaaag
     5101 taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga ggctattttc
     5161 aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg caacgttagt
     5221 cccagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt ttagagcgaa
     5281 caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc ctaaatagtc
     5341 agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc ataacgccag
     5401 ctgccgggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga tgattatatt
     5461 taacccagta aatacgttcc tgtctttttt tgtataaaaa atgtttatta ctcgatgtaa
     5521 ggatacatta attttacgtt tattatgatg aatcctcggc aaatgagcga aatttgatag
     5581 aacatttatc tgctgtacga tttaatgaac ttaatgatac agcattacaa aaacaatcga
     5641 agtttataaa gatgatttct gattgaccag ccccctaccc tacaatggtg attaatttta
     5701 ttactcgcga tggagatgat gatatgaata tatcatatgt gaatagcaac aaaacgacat
     5761 ctttacctgt tgagctagat gcactaaata ataaagatat ctcttatgcc aaagatttct
     5821 cttatgccaa agatttcttt ttatacatag aaacacagtt aaaaattgca aaagattttt
     5881 gtagacctgg agaagaagta tcaagttcta ttgcaagtaa agtttttcac gcatttattg
     5941 atttagttaa caaaatcagg ggtaagaaag actttatgta tatttgcacg ctttgctgtt
     6001 ttgccgagga ggttaaaggt gattattctc attacaggac ctttttattt gatattggta
     6061 atcaatataa ggttaaactt atacaaagcg gaaaaaaaga gttctcttta acattagaat
     6121 ttaacgatac tataattgaa tctcagaaag tcacaggcaa taaggcaaag catattcttg
     6181 aggatataga aatattctat cgtaacaaac ccgatactta ttattaaatt taataatggt
     6241 tagatgtgca gaaattatta ctccacacga aaaaataggg aaaggaattc cagataattg
     6301 ggataaactt aacaataaaa gaactgacaa cgttttatta tctctcacgt agaacaacgg
     6361 catcaaaaaa atcatctaca aagaaatagt gaaagcgacg acagtttaat ttcactgcag
     6421 gctgggtaac tccagcctgc tttcctgcat tacatcaccg cagcaaacgc ctttgccaca
     6481 cgttgtacat ttgccgtatt taacccggcg acacacatgc gaccgctggc gatgagatag
     6541 acaccaaatt cttcacgtag tcggtcaacc tgagcggcac ttaaaccggt ataactgaac
     6601 atgccgcgct gattaagcag ataatcgaaa ttgcgttctg gcatctctgt gcttaatacc
     6661 ttcaccaatt cctgacgcat tgccagaatg cgagtacgca tctcttctac ttccgccagc
     6721 cagctggctt tcaatgcctc gtcattcagc actgcagcca ccacctgcgc accaaaattc
     6781 ggcgggctgg agtagttgcg gcgaactgtt gctttcaatt gccccagtac gcggccagcg
     6841 gcttcggcat cttcacacag aacagaaagt ccgccgacgc gctcgccgta aagggagaaa
     6901 attttcgaga acgaattgct caccagagcg ggtaatccag cgctggcaat ggcgcgaata
     6961 gcgtaggcat cctcttccat accggcacta aatccttgat aggcaatatc gaggaatggg
     7021 ataagctcgc gggctttgag aatttcaatc accgcatccc actgatcatt agtgagatcg
     7081 gcacccgttg ggttgtggca acatggatgc agcaacacaa tactgcgggc aggtaatgtt
     7141 ttcagcgtcg ccaacaggtc attaaagcgc acgctgttag tcacttcgtc ataccagggg
     7201 taagtactca cttcgaatcc agccccggcg aatattgcta cgtggttttc ccaagtagga
     7261 tcgctgaccc agacgcctga ttccgggaag tagcgtttca ggaaatccgc gcccactttc
     7321 aatgcccctg agccgccaag ggtttgaatg gttgctacgc gctgttgttg cagtaccgga
     7381 tggtccgcac caaacagcag cggcgcaatg gcatggcgat agctgttaag cccttccatc
     7441 ggtaaataaa gcgaagcgcc atggggctgc gcattcaggc gcgcttccgc ctccgccacg
     7501 gctttcaatt gtggaataat tccgtcttcg ttgtagtaca gaccgatact taaattcact
     7561 ttgtcgctgc gagggtcttc tttaaaacgc tccataagcg taagaatcgg gtcgccagcg
     7621 taggcgtcaa ctttttgaaa cacgcgatgg ttctccaggt ttacgggcag gtggttaaaa
     7681 cacaataaac cggaagaagg cgaagatcga gtggatgttc aggagcgaac ggcaattagc
     7741 aacagagtga gactaatgat aaacgtacat ccgccagagg cacgaccttt ataagagcat
     7801 gaaagaacat caacttattg aatttttagg attttattgg ccggataagg cattcacgcc
     7861 gcatccggca cagacaatca aatattacag aacgattaat ccacgtattt catcgcgacc
     7921 cttgaagtca ggcgcgtaat aagttcgtaa gcgcttactt tcgtcatttc agcgatacgt
     7981 tctacgggca aaccttcgcc ccataaaatg accggatccc cggctttgtc ctgcgcctgt
     8041 ggacctaagt ctacgcagat catatccatc gccacgcgcc cgacaatcgg tacttcgcga
     8101 ccgttcacca gcactggcgt accggacggc gcggcgcgcg gataaccatc gccatagccc
     8161 atcgcgacta cgccaagacg ggtatcacgt tcgcttaccc aggttccacc ataaccaaca
     8221 ggctctccgg ctttatgctc acgcacggca atcaggctgg aggttagtga catcactggc
     8281 tgacagccaa aatcggcacc ggtggagcga tcttccagcg gcgagacgcc ataaagaatg
     8341 atgcccgggc gcacccagtc aaaatgcgac tgtggccaca gcagaatgcc acccgacgcg
     8401 gcaatggaac gttgaccagg tttgccttcg caaaaggtat taaagatagc gagttgtttc
     8461 tcggttgcgc cacattttgg ttcatccgcg cgcgcaaaat ggctgacgat attcaccggc
     8521 tgacgaacgt ttttgcactg ggtcaggcga tgataaaacg cctcagcctg ttccggcctt
     8581 acgcccagac ggtgcatacc ggtatcgagt ttcatccaga cggtaaccgg ctcgtccaag
     8641 ctagcctctt ccagcgcagc cagctgttct tcgttatgca cggcggtatg aaaatgttgc
     8701 gcagaaatcg tcggcagatc tctggcatca aaaaagcctt cgagtaacag tacaggtttg
     8761 gtgattcccc ccgcacgcag tcgcagagct tcttcgagac gggctacgcc aaaggcgtca
     8821 gcatcgggga gcgttcgcgc ggtctcaaga agaccgtgac cataagcgtt cgctttcacc
     8881 accgcaacca ttttactggc aggtgccagt tcacgaagac gttgcaggtt gtgtcgcaga
     8941 gcgcggcggt taatcacaac agttgccgct tgcatttgtg ttccttgata attgtttgct
     9001 ttaattacct aattcataaa ataattatta ttcgtcgtcg tactgcggcc ccgcatagtt
     9061 gtcgaagcgc gaccattgac cgttaaaggt caggcgtacc gtcccgattg ggccgttacg
     9121 ttgtttaccg ataataattt ccgcgatgcc ttttaaatca ctgttttcgt gatacacctc
     9181 atcacgatag ataaacatga tcaagtccgc atcctgctcg atagagccag attcacgcag
     9241 gtcggagttg accggacgtt tgtcggcacg ttgttccaga gaacggttca actgggacag
     9301 cgccaccacc ggcacgttca gttctttcgc cagtgctttc agcgagcggg agatttctgc
     9361 aatttccagc gtacggttat cggaaagcgc cggtacgcgc atcagttgca ggtagtcgat
     9421 catgataagc ccgatgccgc cgtgttcacg ggcaatacgg cgtgcgcggg aacgcacttc
     9481 cgttggcgtc aggccggaag agtcatcgat atagatattg cgtttttcga gcaaaatacc
     9541 catggtgccg gaaatgcgcg cccagtcttc atcatcgagc tgcccggtac ggattttagt
     9601 ctggtcaacg cgcgacagcg acgccagaga acgcatcatg atctgttctg atggcatctc
     9661 cagcgagaag ataagtactg gtttatcctg caacattgcc gcgttttcga cgaggttcat
     9721 cgcaaatgtt gttttaccca tcgacggacg cgcggcgacg atgatcaaat ccgacggctg
     9781 caagccagcg gtttttttgt tgagatcgtc ataaccggtg tttaccccgg taacgccatc
     9841 gtgtggctgc tgaaacaact gctcaatacg cgccacggtt gcgtcgagca catcggcgat
     9901 gttcttcggc ccttcgtctt tgtttgcacg actttcggca attttaaaga cgcgggattc
     9961 agcaaggtcc agcagatctt cgctggtacg cccctgcgga tcaaaaccgg cttcagcaat
    10021 ctcattcgca accgagatca tctcacggac aacggcacgt tcacgcacga tgtccgcata
    10081 agcactgatg ttcgccgcac ttggcgtatt ttttgacagc tctgccagat aagcaaaacc
    10141 accgacgcta tcgagttgcc cctggcgttc caacgattcc gcaagggtaa tcagatcgat
    10201 aggactaccg ctttcctgca aacgcgccat ttcagtaaag atatgacggt gtgggcgggt
    10261 gtaaaaatcg tctgctacca cacgctcggc tacatcatcc cagcgttcgt tatccagcat
    10321 taaaccgccc aacaccgact gctccgcttc gatcgagtgc ggaggcactt tcagcccggc
    10381 aacttgtgga tcgcgttcgc ggggttcagc ctgctgtttg ttgaagggtt tatttcctgc
    10441 catagtgaat ggagttaccg aaataaagaa tgggtcgaaa ctttaccata tgaagcagac
    10501 cctgacgata cgttctggag gacacatggc aacacgaatt gaatttcaca agcacggtgg
    10561 cccggaagta cttcaagccg tagagttcac tcctgccgat ccggcggaga atgaaatcca
    10621 ggtcgaaaat aaagccatcg gcatcaattt tatcgacacg tatatccgca gcggccttta
    10681 cccgccgcca tcgctaccca gcggattagg taccgaagcg gcaggcatcg tgagtaaagt
    10741 cggcagtggt gtaaagcata ttaaggctgg cgatcatgta gtctatgcgc agtcggcgtt
    10801 aggcgcttac agctctgtgc ataacattaa tgcggataaa gcggcgattc tgcctgcggc
    10861 aatttctttt gagcaacctg cggcatcctt cctgaaaggc ttaacggttt attatctgct
    10921 gcgcaaaacc tatgaaatta aacccgatga acagttcctg ttccacgcag cggctggcgg
    10981 cgttggctta attgcctgcc agtgggcaaa agccctgggc gcgaaactca tcggcaccgt
    11041 aggaaccgcg caaaaagcgc agagcgcgct aaaagccggc gcgtggcagg ttattaacta
    11101 tcgtgaagag aatctggtcg agcgattgaa agagatcacc ggcggtaaga aagtgcgcgt
    11161 agtgtacgat tccgtgggca gagacacctg ggaacggtcg ctggattgcc tgcaacgccg
    11221 cggcttaatg gtcagttttg gcaactcatc aggtgcggtt accggtgtga acttaggcat
    11281 tctcaatcaa aaaggctcgt tgtatgtgac acgcccttcc ctgcaaggct atatcaccac
    11341 gcgggaggaa ttaaccgagg ccagtaatga actgttctct ttgattgcca gcggtgtgat
    11401 taaggtcgat gtcgccgagc agcagaaata tccgctgaag gatgcgcagc gtgcgcatga
    11461 gattctggaa agccgggcga cgcaaggttc cagcctgctg attccataaa agaaataggg
    11521 cttccacctg ggaagccctt tctttttata gttcggctgt atgtagggta cagcacgatg
    11581 aatctgttag aggcgcaata gtgacagatt tgattatcaa ttcctatttt gttctaagga
    11641 taaaacctta ggttgtgatc atccgcacaa tcccttagta acgccagcgg tcataacgct
    11701 gatatttcgg catttttggt gctttaatcg ccttaataac ccacaccacc gcaatcgcca
    11761 gtagtaacca cggcagcaac ttaatcatca atgccagcat accgccgagg aacataatgg
    11821 ccgtcgccac aaccagcgcg gcgataatgc ccagcaacga aacgccggtg accatcagca
    11881 tgacaaaaaa gccaatcaca aaaagtagtt ccagcatgat gctctcccaa atatgaaatc
    11941 tcttgctggc attacaagaa tcatgccaaa aataatctat tgatttaaca gcaaaacgcc
    12001 ccgcgacggt gcgcagggcg tggtgaattt gactactttt tggtgaaaag ttaacgctta
    12061 tccgccacca gtttgagcgc gtgttccagc acgttaatgt cagcacccgc tttatgggca
    12121 ttttcactta aataacgccg ccactgccgc gcgccaggaa taccctggaa caaacccagc
    12181 atatgccgga taatatggcc gagatacgtc ccctggctga gttcatgctc aatgtacgga
    12241 tacatggcac gcactactgc caccggatcg gcatcggtat ccgaggaacc aaagatctcc
    12301 cggtctaccg ccgccagaat acccggattc tgatacgcct cgcgcccgac catcacgcca
    12361 tccatatgtt gcaaatgcgc tttagcttct tccagcgact tgataccacc gttaatcgac
    12421 attgtcagat gcgggaagtc acgcttcagt tgatacacac gcggataatc gagcggcggg
    12481 atttcacggt tttctttcgg gcttaacccc gaaagccagg ctttacgtgc gtggatgatg
    12541 aacatctcac actcgccttt gccggaaacg gtattgatga aatcgcagag aaattcatag
    12601 ctgtcctggt cgtcgatgcc aatacgcgtt ttcaccgtca ccggaatcga caccacatcg
    12661 cgcatcgctt tcacgcagtc ggcaaccagc tgcgcattac ccatcagaca cgcaccaaac
    12721 atgccgttct gcacccggtc agacgggcag ccgacattca ggttgatctc atcatatccg
    12781 cgcgcttctg ccagctttgc acactgcgcc agcgccgccg gatcgctacc gcccagttgc
    12841 aacgctaccg gatgttcttc ttcactgtac gccagataat cacctttacc gtgaataatc
    12901 gcccctgtgg tcaccatttc ggtataaagc aacgtattgc gggaaagcag acgcaagaaa
    12961 tagcggcaat gtctgtccgt ccagtcgagc attggtgcaa cactaaaccg accactccag
    13021 tgaacgtcag ttttttcagg cattgcgctg gtttggttga ttttttgcat ttcagaatta
    13081 ccgtgcattt tcaaatgtag agattatttt atcgatatat catggggtta tgttattcag
    13141 cttcactgtt caggaggctc aatagcgggg tactatacca taacaacagg aagcgcctgt
    13201 ctcattgcaa aagaaaattg agatcctctc aaggcatgaa gctctcacga agtgatggaa
    13261 ataatcttat tagccgttaa ctttgttaag gccaatgata aacaatccag gttcgacgat
    13321 aaataaaaaa tcacacatta aactctggtg atatatctcc ctgctaatag cattgataga
    13381 gaaaaaagaa cccaataaat attgggtcct tttatataat gccttccata ctattgaaga
    13441 acttcacata ttatttctcc gatttaaccc cgaacaagtc ataaattaat ttagaagtgt
    13501 ctgtaagtat tttaatctct tcctttgagg ttgggtcaaa cgacttcgcg aagctaatta
    13561 attgtggtgc agcatctcgc attttgctta aaatatcagg atcgagcgtt ccttcattca
    13621 ccagatgtga catcttatcc agatagccat caaaattcat ttctccccca tctgccagac
    13681 gcttcatctc tcccaggtac ttcttcatat cacgttgata taatttttca aactgagctt
    13741 tcaggtagct ctcattattt tcattaatat acactgtttc tgataaatct gcgtaggcac
    13801 tagaatatga aagtgaaatt aacagcgaag caaataattt taagctgttg tcatcatgtt
    13861 cctggcaggc attaacaaac gttaaaaaac cagaacctat tgcttgaaaa tgtatattcg
    13921 ctaaaggttc attatcctta gattcctcat aaaatagctg aaccgtggag ggaaggccat
    13981 tcttagtttc tacatcaaaa gtacattgtt caatgggcat tgcatcttta ttatccccag
    14041 gattacatat actattaatc gcgaaatgaa aaatagcccg gtcaacaact gatgcaataa
    14101 agtcacgatc gtttaaatcc tcatcagtgc aatcgtcagt atacttattg acccacattt
    14161 tgagatcatt tatttgctca attaaaaaca cattttttta cgtgcatgcc ataaattatg
    14221 cgtgattcgt tgaataaaca aatctatttt tgcaataatt atatcccgat gacgcagcgc
    14281 tgcataatca atagatttac catatatatt atttaattta cctttgattg aacctctatt
    14341 atccaattta tctttggttg catctttatt ttcgaattga tttgaattga gtgttttatt
    14401 aaagtatcca gttgcaccgg gtattatctt caacatgatt catctacctt ttttgtcgta
    14461 ccgtgtcctc cttataattc ttttgccatc tggtcactat caaaaatcgc tcataatttc
    14521 tgaactacag gtatgcgttt aaacccagtt aaataaatag cgagttaact tgaagcaatt
    14581 tatttaaaaa tgaattatta attattatcg tgttgataat ggtacgacaa ggcaacacaa
    14641 caccttacct acaaaacaga aaatcaacat tacggcaaca ataagggttc tcggtgtttt
    14701 tcctggaaca tggtaaagta aggacattct taacccccac tttgaggtgc ccgatggaaa
    14761 agaccacaac gcaggagtta ttagcgcagg ctgaaaaaat ctgcgcgcag cgtaatgtgc
    14821 gcctgacccc acagcgcctg gaagtgttgc gcctgatgag tctgcaggat ggcgctatca
    14881 gcgcttatga tctgcttgat ttactgcgcg aagctgaacc gcaagccaag ccgccaacgg
    14941 tttatcgcgc gctggatttt ctgcttgagc aaggttttgt gcataaggtg gaatccacca
    15001 acagttatgt gctctgtcat ctgttcgacc agcccacgca tacgtcagcc atgtttattt
    15061 gcgatcgctg cggcgcggtg aaagaagagt gtgcagaagg cgtggaagac attatgcata
    15121 cgctggcggc aaaaatgggg tttgccctgc ggcataatgt gattgaagca catgggctct
    15181 gtgcggcatg cgtggaagtg gaagcgtgtc gtcatcctga acagtgccag catgatcact
    15241 ctgtgcaggt gaaaaagaaa ccgcgttaag agggcgtaca tccttgtaca cgtcgggcag
    15301 gagggattaa ttaccagcga tattcattgc gggtttccca atccacgacc tctttttctg
    15361 cctgatcttt ctgataacca taacgttcct ggattttacc gaccagttga tcacgtttac
    15421 cttcaatgat cgtcatatca tcatcggtca gtttgcccca ttgctctttc actttacctt
    15481 taaactgttt ccagttaccg ccggcttcat ctttattcat aatcaagacc tcatcgttag
    15541 gttgtgaatg agagtacgtt cacttttctt ctgaacgtga gattaagtgt agtcagcaat
    15601 ttggcttagg ataatttatt cagaattttt aaccgtcacg ttgcggcaaa ccaggtaccg
    15661 ttgcgccagt gacgccgcca gatagccgcc agagaaagcc cgcgcaacgc cagaaagacg
    15721 gttaatgcca gccacaaacc atgattaccc agccacggca gcgtaaggag cgtcagcgca
    15781 aaacctgcgg cggccaccgc catactgtta cgcatttcgg cggcacgcgt tgcgcctata
    15841 aacatgccgt ccagcagata acaccagacg ccaaccaacg gcaaaatcac ctgccagata
    15901 agatagcggt cagccagctg ctgaatctgg gttaacgacg tcagcaacgc aatgatgtgt
    15961 tcccctgcca gcaaataaac caccgaaaac agtaacgcta caatccccga ctggcggcac
    16021 gctgcccgcc atacatccag caactggcta ccgtcgcgcg caccgtatgc ctgaccggag
    16081 tgcgcttcaa ccgcgtaggc aaaaccatca agcgcatagg cggtaaaggt gagtagcgtc
    16141 atcagaaccg cgttaacagc gataatgtca ctccccagtc gcgcaccaag tacggtgatc
    16201 gcgccgaaac agagttgcaa caacagcgaa cgcagcatga tatcgcggtt aagcgccagc
    16261 aagcgacgga agtttcctcg ccaggcagtt ttcagcattt cgccggaaat tccgcgtagt
    16321 ttgaggattt tacgcaccat tagcagacca atcagcaatg ttgcatattc cgcaataacc
    16381 gtcgccagcg ccgcgccctg cacgttcata tgcagcccca tcaccagcca gacatccagc
    16441 acaatgttga ggatattgcc gaccactaac aaaattactg gcgcacgggc atattgcacg
    16501 ccgagtaacc aaccgagtaa taccagattc gccagcgacg ccggagcgct taaccagcgg
    16561 atttcaagaa agcgccgcgc ctgttccagg accgcttcac taccgccaac aatatgcagc
    16621 gccagatcga taatcggcgt acgcaacagc gcaattaacg ccccggcccc caacgccaac
    16681 agcaacggtt gcaccagcgc acgggctaat gcctgaggat ttttggcacc ataagcctgc
    16741 gcagtcagcc cggtggtgct catgcgtaaa aacagcaaca gcataaagag aaagctggtc
    16801 gccgttgcac caaccgccac gccgcccaaa taaaccggac tatcaagatg accaattacc
    16861 gccgtatcga ccagtcccag caacggaacg gtgatattgg agaaaatcat gggtaaggcg
    16921 agatgccaga gtgctttatc agatgaagtg aggaatgcca tgcagacaag cctgatgaag
    16981 agagatgaaa aacaaaccgc gacgccaggc ggcatcgcgg tctcagagat atgttacagc
    17041 cagtcgccgt tgcgaataac cccaaccgcc agcccttcaa tggtgaagct ctgctgacga
    17101 aggtcaacga caattggttt aaactcgcta ttttctggca acagttcgac tttattgccc
    17161 tgttttttca ggcgcttaac ggtaacttcg tcatcaatac gtgcgacaac gacctgaccg
    17221 ttacgtacat cctgagtttt atgcactgcc agcaagtcgc catccataat gccgatatct
    17281 ttcatcgaca tcccgctgac gcgcagcagg aaatcagcat tcggcttgaa caaggaagga
    17341 tcgacctgat aatgaccttc aatatgctgt tgcgccagaa gcggttcacc ggcagccaca
    17401 cgacctacca gcggcaaccc ttcttcctct tcctgcaaca gacgaatccc gcgtgatgcg
    17461 ccggaaacaa tttcaataac gcctttgcgt gccagcgcct tcagatgttc ttcagccgcg
    17521 tttggggaac ggaaccccaa acgctgcgcg atttccgcac gcgtcggcgg catacctgtc
    17581 tggctgatgt gatcacggat gagatcaaac acctcttgtt gcctggccgt taacgctttc
    17641 attccgcccc ctgggtgtat atacagttat actgtgagta tatacagcaa aaggcgattt
    17701 tggaaccata aactgcacaa taaaccagag atttatcgaa atctggaagg gttatccaaa
    17761 atgcgaccat aacaggatgc accaggtaat cacggcgacg ataatggcaa tcagcaccgc
    17821 ggcggatccc atatcttttg cgcgtccgga aagctcatgg tattcagagc caattcggtc
    17881 aaccactgct tcgatggcgc tattgaggat ttccacaatc atcaccagca tcacggagct
    17941 gataagcagc acgcgggtaa ttgcgtccac atccagccag caggcgatga ccaccgccaa
    18001 caataccgct acgccttcct gacggaatgc cgcttcgttg atccatgcag cgcgtaaacc
    18061 tttccaggaa tagccagcag ctttgataat tcgggtgaat ccagtggtat tattggccat
    18121 tgaaagaacc tttttacatt atgagcgtca atatcagtgt accgttaaat ccgatgattc
    18181 gggtttgcgt aacgccgcgc gaaacatgag cggataccac agaatttccc atgactttct
    18241 gctatccttg ccgcgcattt gcattattaa ccagaggctt tacatcgttt atgtccggct
    18301 ggccacgaat ttactacaaa ttactgaatt taccattaag catcctggta aaaagcaagt
    18361 ctattccggc agatcctgcc ccggaactgg ggctggatac ctctcgtcca attatgtacg
    18421 ttttaccgta caactcgaaa gcagatttgc tgacgttgcg cgcccagtgt ctggcacatg
    18481 atttgcctga cccgttagag ccgctggaaa tcgacggcac gctactgccg cgctatgtgt
    18541 tcattcacgg cgggccgcgt gtattcacct attacacgcc gaaagaagag tctattaagc
    18601 ttttccacga ctatctcgat ttgcaccgta gcaacccaaa tctggatgtg cagatggtgc
    18661 cagtatcggt gatgtttggt cgcgcgccgg ggcgtgaaaa aggcgaagtg aacccgccgc
    18721 tgcgtatgct taacggcgta cagaaatttt tcgctgtact gtggctcggt cgcgacagtt
    18781 ttgtgcgttt ctcgccgtca gtgtcgctgc gccgtatggc ggatgaacac ggcacggata
    18841 aaactatcgc tcagaaactg gcgcgcgtgg cgcgtatgca ctttgcccgt caacgtctgg
    18901 ctgccgtagg cccacgtctg cctgctcgtc aggatctgtt taataagctg ctcgcctccc
    18961 gcgccattgc caaagcggta gaagatgaag cgcgcagcaa aaaaatctcc catgaaaaag
    19021 cgcagcagaa cgcgattgcg ctgatggaag agattgcggc gaatttctct tacgagatga
    19081 tccgcctgac tgaccgcatt ctgggcttca cctggaaccg attttatcag ggcatcaacg
    19141 tccataacgc cgagcgcgtt cgccagctgg cccacgacgg tcatgagctg gtgtatgtgc
    19201 cttgccaccg cagtcacatg gactacctgc tactttctta cgtgctgtac caccaggggc
    19261 tggtgccgcc gcatatcgcc gccgggatca acctgaactt ctggccggcc gggccgattt
    19321 tccgccgtct gggggcgttc tttattcgcc gtacttttaa aggcaataaa ctttattcca
    19381 ccgttttccg tgagtatctc ggcgaactgt tcagccgtgg ttattccgtc gagtacttcg
    19441 tggaaggcgg tcgttcccgc acggggcgtt tgctggatcc gaaaaccggt acgctgtcga
    19501 tgaccattca ggcgatgctg cgtggcggca cgcgtccgat tacgctgatt ccgatctata
    19561 tcggttatga gcacgtcatg gaagtgggta cttacgccaa agaactgcgc ggcgcgacga
    19621 aagagaaaga gagcctgccg cagatgctgc gcggtttaag caagctgcgt aatctcggtc
    19681 agggttacgt caacttcggt gaaccaatgc cgttgatgac ctaccttaac cagcacgtac
    19741 cagactggcg tgaatctatc gatcccatcg aagcggtgcg tccggcctgg ttaacgccga
    19801 cggtcaataa tattgctgcc gatctgatgg tacgcattaa caacgcaggc gcggcaaacg
    19861 ccatgaacct gtgctgtact gcgctactgg catcacgtca gcgctcactc acccgcgagc
    19921 agttaaccga gcaactcaac tgctacctgg atctgatgcg caacgtacct tactccacgg
    19981 actctaccgt tccttcagcc agcgccagcg agcttatcga tcacgcgctg caaatgaaca
    20041 agtttgaagt cgagaaagac actatcggcg acatcatcat tctgccacgc gagcaagcgg
    20101 tgctgatgac ctactatcgc aacaacattg cgcatatgtt ggtgctgcct tcgctgatgg
    20161 cggcaattgt cacccagcat cgccacatct cccgcgacgt attgatggag cacgtcaatg
    20221 tgctttaccc aatgctgaaa gcggagctgt tcctgcgctg ggatcgcgac gagttgccgg
    20281 acgttattga tgcgctggca aatgagatgc aacgtcaggg gctgattacc ctgcaagatg
    20341 atgagttgca tatcaacccg gcgcattctc gcaccctaca gctgctggcc gcaggcgcgc
    20401 gcgaaacgct gcaacgttat gccatcacct tctggttgtt gagtgccaac ccgtcgatca
    20461 accgcggtac gctggagaaa gagagccgca ccgttgcgca acgtctctcc gtgctgcacg
    20521 gcatcaacgc gccggagttc ttcgacaagg cggtgttcag ttctctggtg ctgacgctgc
    20581 gtgatgaagg gtatatcagc gatagcggcg atgccgaacc agcagaaacg atgaaggttt
    20641 atcagttgct ggcggagttg attacatcag acgtgcgttt gacgattgag agtgcgacgc
    20701 agggcgaagg gtaatcagag agaattgccg gatgcggcga aaacgcctta tccggcctac
    20761 catgacctgc aaattcaata aattgcgatt caccgggtag gcctgagaag cgcagcgcat
    20821 caggcaaccc agaagaaagc cggatgatca tccggctttt ttacatcatc agaaatgcca
    20881 gtaactcatt gccagtccta aaaacagtac cagaccaaca tagttattat tcataaatgc
    20941 tttaaagcag gcttcacgct cgcggttggc aatcagtttt tgttgataaa caaacagcgc
    21001 gccagccacc acaattgacc agtaatatcc ccagcctaag ccatttaact caccgatgat
    21061 cgccatcagt gccagtacgc caatctgcaa aataccaata atcaatttat cgtattggcc
    21121 gaacaggatt gccgtggatt taatgccaat cttcacatca tcatcgcggt caaccatcgc
    21181 atactgcgtg tcgtaagcca ccgcccagag aatattggcg aggaacatta accagcaact
    21241 caatggcacc gactcactca cagcggcaaa agccattgga atcgaccagc caaacgccgc
    21301 gcccagcacc acttgcggga gatgggtata ccgcttcata aacgggtaca cccacgccaa
    21361 tgccagagcg gcaatcgaca acagaatggt catcgtattc agcgtcagaa ccagtaaaaa
    21421 cgaaatcagt accagcacta caaacagcgc gcgcgcctct ttctctgtta ccgcgccgct
    21481 gggaagtggt cggttcgccg tgcgcttaac atgaccatca aacttgcgat cagcataatc
    21541 attcaccacg caaccagcag cgcgcatcag ccagacaccc gcgacaaaca ccgccaggat
    21601 ccagagctga ggaacgcccg gtgtcgccac ccacaacgcc cataatgttg gccagagcag
    21661 caataacgcg ccaattggct tatccgtacg cattaagcga tgaaacgcca gcagcttatt
    21721 ctgcgtcaga ctccactcca tatttttttc ctcttagtac aacggtgacg ccggtaaaaa
    21781 cagttctgtt agcaacagcg gtttaccgct taatcgcagg cgggaacgtc gcccccacag
    21841 tccggcatca cggcctatct cgataaaatc ccgggttagt gtcgatgatg tgaacagata
    21901 gcgtcctaac ggcgttttac ccaatttttg taacgctagt tccggcccgc ttaatgttga
    21961 tacaggaacg acggtacgac cggcaagcca cggttcacca tcggcacata acaaaatttc
    22021 acgtaaccag taacgagact ctttcggcag tagcggcagt tcttcgggga tttcattctg
    22081 ctcgacaaac ccctcgcgga tcatcgtcac actcaccgtt tttccctgct gttcaaaacg
    22141 ttttgtcatg gaatcctcca gcaacagcca gtcgaacagt tgcggatcca gggcagggat
    22201 ctctttaaaa tagcgcagcg cacgcagttg cgttaacgcg gggtgtgaca tgccgaactc
    22261 tccgttacat aacgtaaagg cattgtatcg cagaaaagtg tttacgggag atgtaaagca
    22321 aattaagtga tctaatgcgc aacaaaaacg caacaatcgc atgaagtggt aaaacgctgt
    22381 tgaaccttac gctttgccga tgcccgtgct ggcgagggca accatagggg catcaccgcc
    22441 catgatgcta ggaacggtga tgttttcctt tacttaaata cctttattct ttatacgagt
    22501 agctcgccaa ctcgcagtaa aaattgggaa aagcgcatat ttaccatgag atttggagct
    22561 gaaaagtgtt tcctgagtaa gattaatagg caacatctta tgtctggatt tatagataca
    22621 cgtggaattc caattggtat cttttgtagg attcaacata tcatgtaagc gctgggagct
    22681 taccaccata acaggcccat catcgacaga ggagaacagg ataaatatat tatcgtttcg
    22741 tgttaattta ttagtgttaa aggttcttaa cttttcttca gtttctataa atataggtga
    22801 aacatcatca ctacgaatcc actctttata aagataccag gcatttgctt tgatcatatc
    22861 atccgttgat cgtaaggcct gatcgataag ttggataaaa aaattgttat ttttatcaac
    22921 cagcccttta ttcatattaa atctgttaat aaaatggatc atctgttggg tatcgaaatt
    22981 caccttactg aatcgatgtt gacgcataaa attatcgaac ttgtcatctt ccccagaatg
    23041 tatataacta cgtataattg gatcttttat ataaggacaa tggctaaatg ttttcttcat
    23101 attctcaata aagtttttat taacaatatt gagaattttt aattgcatca ccagttttac
    23161 tgccagaacg gatttcaata tcattgcatt atctggcaat gtatcaatca tttttataat
    23221 gttattgata taaccatcga gatcttcatt cccaaatata tctgaataat caatagttaa
    23281 ttccaattgt tctgtaatgc aatcttaaaa attccctgtc gacattcgca catgatcaaa
    23341 cataacagct gtcaaattcg atttaataaa tgatatttct ttggcagaca gaaactctgc
    23401 tctggtgaag tgcgaagcgt aaagataaca attatcgaag tttgaatttc taatctgaca
    23461 ttcgcgaaaa ttactatgac caagttgact ctgcgagaag tcaactgtat caagaatatt
    23521 ttggctaaaa tcaacacatt cgccactcgt tttgagaaaa atcacattgt gtaaacgtcg
    23581 acatttaaat atgacattat gaactgttgc cttagtaaaa ttgcaattct caatttcttt
    23641 acattcgaaa gttacatgtt gtagatttga tgataaaaaa gacaccttag aaataacgtc
    23701 acctttaaaa tgacaatttg ttaaatcaaa atcagacagg tcgatatacc cctgcgcatc
    23761 tttatccatc agaaaattac aagcgcattc tattttttca tttctgtaga attttacact
    23821 tatagtatca ttactaattg agacttcacg taacatattt attgcatgat ttttttcgtt
    23881 ccgttgtttt atatttaaaa atatatcact gatataaata aagttatctc tgtttaaacc
    23941 tgtcacccca ttgaaattac gtagtaaatc aagatctact atattatttc cttgcaccac
    24001 atcatgtttc atcaaagagt tattgccgcc agaatttgtg atatgaccta tcataaaacc
    24061 tctgtaatac agaaataaaa taacctggaa aagtagcctg agcgtccaga cactaccggc
    24121 gaaaggaaaa gaatctctga taatgcattg aaataatgga cattcttagt gaagaagaag
    24181 tgaagtacta taaaaaatcg ctcatatccc aggaaggaag cataaaaaaa ggtgcgccag
    24241 gagacgcacc agttgttgca aaatcagcac tgtggggcgt aattacccct tgccttttac
    24301 actgctgata aaggtggaac gggcagatgt cgatcccagg cgttcagctt catcaagcag
    24361 ttttaacgcc ttatcaacat cacctttcgc gacagcgttt ttgatcgcgg tattaaaata
    24421 actttccgtg tcgttgagca tcggctcgct tttcttcacc ggcaccggag caggtgcagc
    24481 cacagctggt gccgccgtgt tgcctaccgt aaccggagct ggagcggaag aaccaaatag
    24541 tggtcctacc aacacgctgg agctggagtt cgttttgact ttcagtttca gtaagccatc
    24601 gctggtatga cgagcaaccg gatcgggaat atccgggatc gagttaccga cgcctttggc
    24661 ataggcttta gccgggtcga gcagttgggt cgtctgctgg agatcttttt ccatggtaaa
    24721 gaccagaaca taaagttttt gctgccccaa cgccggtgtc aggcgcataa cgccttccag
    24781 ccgatctgca ctcatcacgc ctagttcctg gtaggtgaaa taactgctgg ggaagaaggc
    24841 tgatggggtc atgttctgat caataatcag cacgttcggc gcaaaaacgc tggtttgttt
    24901 gttcacttcg ctggtcagcg tcagggtcag ttcgccaatg tttgccggga cgctgtacgc
    24961 agcaaccgga ccactgatgc cgggaacgct cagttgttgg ccgccggtcg ccagttgggt
    25021 ggtctgggtt ttagattgat cgaccggtgt ccaggtgagt tgttgcagcg cggcagatgg
    25081 aatggctggc gcgtcgctgg tgttttgcgg tacgtagtta acatcggcaa ggctaattcc
    25141 aggcgcgctt gccagtaacc ctgctgataa acagagggcg atgagacttt tattcatttt
    25201 cattgttttc acctcaaaat ctggagctca gcggtagcca ggcaatagcg cgctaaaccc
    25261 gataatcaga ggggctttcg cccctttcag ataatgacaa cctgttttta tgccggatgc
    25321 ggcgtaaacg ccttatccgg cccaggtttt gctattacca ccagatttcc atctgggcac
    25381 cgaaggtcca ctcgtcgctg tcgccacgac cgaagctgcc gccgttgaaa tcagcaggaa
    25441 cggctttgcc gtagttcggg ttaaccttgc tatcgccgtt gtagtcgtaa ccccatttct
    25501 catcccactt ggcgtaggtt gcgaagacac gaatagccgg gcgtgaccag atgctgtcgc
    25561 cagcctgcca ttgttgtgcg agggtaattt tgtactgatt gttcttgtcg ccggtgcgct
    25621 gggattcgac gttgtcgtag ccgatttcca tcacggtgct catgattggc gtccacttgt
    25681 acatcgggcg aataccaacg gtccaccact tggtgccgtt gtcgttatcc cagttgatat
    25741 cctggtacat acccacgtac atcatgtccc agttgtcgcc catggagatc gcaccgtggt
    25801 cgaggatacg cagcatgtga ccgttgttgt tgatattgta ggcaaatttt tcgttatcaa
    25861 acgcgacgcc agaaccctgc gacagacctt taccctgcga ggtcatcgag tcagtagcgt
    25921 actgaacaac aaacttgtta aagcccttca ggacactctg agtatgttca gcagtgaata
    25981 accagccgtc tttcgatgcg ccatcaacca gacgatagtt atcacgcagg ttggcacgac
    26041 cgtagtcgac acccagttct aatgtgccgc ccgggttgat ttccatctgc gctaaacgca
    26101 catcgaaaac gtcgttcgcg gtttcgttgg tatagtcata aatattgttg ctggcaaaag
    26161 aggaagaacc accagcttca gaggagcggg ttgctgccag agagagttta ccgaagccaa
    26221 catcgatgtt ttccagaccg gcaccaggac cagaaatatc ccagtagtag aagtcgatca
    26281 tatgaacgtc atgacgttgg tagaagcgct tacctgccca gatggtggaa cctggcagcc
    26341 attcgatcag gttttttccc tgcacgtttg cttcacggaa ggccggatcg gtagcttccc
    26401 agtcattctg ttgcgcgacg gaataggcca cgttagtgtc gaaatagaag ctcttatcgc
    26461 cctctttcca cacttcctga cccaatttta attcagcata agtttcacat tcgttgccaa
    26521 gacggtattt actttgagca ccggtagtct ggaaacactg ttgttcaccg ccgctacctg
    26581 tccagccaat accggaacgt gcatagccgt ggaaatcaac agccattgcc tgagcagaca
    26641 ttacgcccgc tgcgacggca accgccagag gaagtttgcg cagagtaatc atcattctat
    26701 ctcctgagtc attgcttttc ttttttcaca tcacctgtga caggctttgt gtgttttgtg
    26761 gggtgcttaa acacccggct ccttatgcag tcgacgacat gcagtgccat cctcacggaa
    26821 cagatggcaa cgctctggcg gcaggccgat agcgaatgtg gcaccttctt ctaccaacac
    26881 cacgtcgttc tggcggtaca ccaggttttg acgaatggaa gggatctgga tatagatttg
    26941 ggtttcgttg ccgagttgct cgacgacctg aacttcaccc tcaaggatga cgtcagcgat
    27001 atcactcggc agtagatgtt ccgggcgaat acccagcgac atattggctc caacctggac
    27061 atcacggctt tcaacaggca gccagacttg ttgacgattt ggcatcggca gctccacctg
    27121 cacttgatcg attgcggtgg cggtcacttt taccggcagg aagttcatct ttggcgaacc
    27181 gataaatccg gcgacaaaac ggtctgccgg atagtggtac agttccagcg gtttcccaac
    27241 ctgtgccacg cgaccggcgt ccagcaccac gattttgtcg gccagcgtca tcgcttcgac
    27301 ctgatcgtgg gtgacgtaaa tcattgtgcg gcccaggcgt ttatgcagac gggagatttc
    27361 gatacgcatt tgcacacgca gtgcagcatc gaggttggag agcggttcat cgagcaaaaa
    27421 tacgcttggc tcggccacca gcgtacggcc aatcgccacg cgctgacgct gaccaccgga
    27481 gagcgctttc ggtttgcgat ccagcaaatg cgccagttgc agcacttccg ccacctggtt
    27541 gacgcgttgg ttaatcacct ctttttttgc gccagccagt ttcaggccaa atgacatgtt
    27601 ttctgctact gacaggtggg gatagagcgc gtaagactga aacaccatac caacgccgcg
    27661 ttctgctggc ggagtgtcat tcatccgttt ctcaccgatg aacaggtcgc cgctggtgat
    27721 cgtctcaagc ccggcaatca tgcgcagtaa agtcgattta ccgcagccag acggtccgac
    27781 aaacaccacg aattcacctt catggatatc gagattgata tctttcgata ccacgacctc
    27841 gccccaggct ttcgttacat tttgcagctg tacgctcgcc atgcccttct ccctttgtaa
    27901 caacctgtca tcgacagcaa cattcatgat gggctgacta tgcgtcatca ggagatggct
    27961 taaatcctcc accccctggc ttttttatgg gggaggaggc gggaggatga gaacgcggct
    28021 tctgtgaact aaaccgaggt catgtaagga atttcgtgat gttgcttgca aaaatcgtgg
    28081 cgattttatg tgcgcatccc cacattaccg ccaattctgt aacagagatc acacaaagca
    28141 acggtggggc gtaggggcaa ggaggatgga aagaggttgc cgtataaaga aactagagtc
    28201 cgtttaggtg ttttcacgag cacttcacca acaaggacca tagattatga aaataaaaac
    28261 aggtgcacgc atcctcgcat tatccgcatt aacgacgatg atgttttccg cctcggctct
    28321 cgccaaaatc gaagaaggta aactggtaat ctggattaac ggcgataaag gctataacgg
    28381 tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca ccgttgagca
    28441 tccggataaa ctggaagaga aattccctca ggttgcggca actggcgatg gccctgacat
    28501 tatcttctgg gcgcacgacc gctttggtgg ctacgctcaa tctggcctgt tggctgaaat
    28561 caccccggac aaagcgttcc aggacaagct gtatccgttt acatgggatg ccgtacgtta
    28621 caacggcaag ctgattgctt acccaatcgc tgttgaagcg ttatcgctga tttataacaa
    28681 agacctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg ataaagaact
    28741 gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact tcacctggcc
    28801 gctgattgct gctgacgggg gttatgcgtt caagtatgaa aacggcaagt acgacattaa
    28861 agacgtgggc gtggataacg ctggcgcgaa agcgggtctg accttcctgg ttgacctgat
    28921 taaaaacaaa cacatgaatg cagacaccga ttactccatc gcagaagctg cctttaataa
    28981 aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg tccaacatcg acaccagcaa
    29041 agtgaattat ggtgtaacgg tactgccgac cttcaagggt caaccatcta aaccgttcgt
    29101 tggcgtgctg agcaccggta ttaacgccgc cagtccgaac aaagagctgg cgaaagagtt
    29161 cctcgaaaac tatctgctga ctgatgaagg tctggaagcg gttaataaag acaaaccgct
    29221 gggtgccgta gcgctgaagt cttacgagga agagttggcg aaagatccac gtattgccgc
    29281 caccatggaa aacgcccaga aaggtgaaat catgccgaac atcccgcaga tgtccgcttt
    29341 ctggtatgcc gtacgtactg cggtgatcaa cgccgccagc ggtcgtcaga ctgtcgatga
    29401 agccctgaaa gacgcgcaga ctcgtatcac caagtaatgc tgtgaaatgc cggatgcggc
    29461 gtgaacgcct tgtccggcct acaaaactga aacgcatgta ggcctgataa gacgcatcag
    29521 tgtcgcatca ggcagttgtt gtcggataag gcgtgaaagc cttatccgtc ctggaatgag
    29581 gaagaacccc atggatgtca ttaaaaagaa acattggtgg caaagcgacg cgctgaaatg
    29641 gtcagtgcca ggtctgctcg gcctgctggt gggttacctt gttgttttaa tgtacgcaca
    29701 aggggaatac ctgttcgcca ttaccacgct gatattgagt tcagcggggc tgtatatttt
    29761 cgccaatcgt aaagcctacg cctggcgcta tgtttacccg ggaatggctg gaatgggatt
    29821 attcgtcctc ttccctctgg tctgcaccat cgccattgcc ttcaccaact acagcagcac
    29881 taaccagctg acttttgaac gtgcgcagga agtgttgtta gatcgctcct ggcaagcagg
    29941 caaaacctat aactttggtc tttacccggc gggcgatgag tggcaactgg cgctcagcga
    30001 cggcgaaacc ggcaaaaatt acctctccga cgcgtttaaa tttggcggcg agcaaaaact
    30061 gcaactgaaa gaaacgaccg cccagcccga aggcgaacgc gcgaatctgc gcgtgattac
    30121 ccagaatcgt caggcgctga gtgacattac caccattctg ccggatggca acaaagtgat
    30181 gatgagctcc ctgcgccagt tttctggcac gcagccgctc tacacactcg acggtgacgg
    30241 cacgttgacg aataatcaga gtggcgtgaa atatcgtccg aataaccaaa ttggctttta
    30301 ccagtccatt accgccgacg gcaactgggg ggatgaaaag ctaagccccg gttacaccgt
    30361 gaccaccggc tggaaaaact ttacccgcgt ctttaccgac gaaggcattc agaaaccgtt
    30421 cctcgccatt ttcgtctgga ccgtggtgtt ctcgctgatc actgtctttt taacggtggc
    30481 ggtcggcatg gttctggcat gtctggtgca gtgggaagcg ttgcgcggca aagcggtcta
    30541 tcgcgtcctg ctgattctgc cctacgcggt gccatcgttc atttcaatct tgattttcaa
    30601 aggattgttt aaccagagct tcggtgaaat caacatgatg ttgagcgcgc tgtttggcgt
    30661 gaagcccgcc tggttcagcg atccgaccac cgctcgcacg atgctgatta tcgtcaatac
    30721 ctggctgggt tatccgtaca tgatgatcct ctgcatgggc ttgctgaaag cgattccgga
    30781 cgatttgtat gaagcctcag caatggatgg cgcaggtccg ttccagaact tctttaagat
    30841 tacgctgccg ctgctgatta aaccgctgac gccgctgatg atcgccagct tcgcctttaa
    30901 ctttaacaac ttcgtgctga ttcagctgtt aaccaacggc ggcccggatc gtcttggcac
    30961 gaccacgcca gccggttata ccgacctgct tgttaactac acctaccgta tcgcttttga
    31021 aggcggcggg ggtcaggact tcggtctggc ggcagcaatt gccacgctga tcttcctgct
    31081 ggtgggtgcg ctggcgatag tgaacctgaa agccacgcga atgaagtttg attaagggag
    31141 ataacaaaaa tggcaatggt ccaaccgaaa tcgcaaaaag cacgtttgtt tattactcac
    31201 ctgctactgc tactttttat cgcggcgatt atgttcccgc tgctgatggt cgtcgctatc
    31261 tcgctgcgtc agggaaactt cgcgaccggt agcctgatcc cggagcaaat ctcctgggat
    31321 cactggaaac tggcgttagg ttttagcgtt gaacaggctg atggtcgcat tacgccaccg
    31381 ccattcccgg tactgctgtg gctgtggaac tcggtaaagg tcgctgggat ttccgcgatt
    31441 ggcattgtgg cgctctccac cacctgcgcc tacgctttcg cccgtatgcg ctttccaggc
    31501 aaagcgacgc tgctgaaagg aatgctgatt ttccagatgt tcccggcagt actttcactg
    31561 gtcgcgttgt atgcgttgtt tgatcgtctg ggtgagtaca ttccattcat tggcctgaat
    31621 actcacggcg gcgtaatttt cgcgtatctg ggtgggattg cgctgcatgt ctggaccatc
    31681 aaaggctatt tcgaaaccat cgacagttcg ctggaagaag cggctgcgct ggatggtgcg
    31741 acaccctggc aggccttccg ccttgtcctg ttgccgctgt cagtaccgat tctggcggtg
    31801 gtattcatcc tgtcgtttat cgctgccatt actgaagttc cggtcgcgtc gctgttactg
    31861 cgtgacgtaa acagctacac cctggccgtg gggatgcagc aatacctcaa cccgcaaaac
    31921 tacctgtggg gtgactttgc cgccgctgcc gtgatgtctg cattaccgat caccatcgtc
    31981 ttcttgctgg ctcaacgctg gctggtcaac ggcctgacgg caggtggtgt gaaaggttaa
    32041 agatgttgtt ctgccaatgt tatgccgctg caccctcaac ttacgttatc ccaacttgtg
    32101 actattattc ggcgctccac ggagcgcctt ttttctttcg tctgcaatct gaatcgttcg
    32161 ccggttaata ttccca
//
LOCUS       DAJTNM010000033        32000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTNM010000033 DAJTNM010000000
VERSION     DAJTNM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.033"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..167)
                     /locus_tag="OO172_001654"
     CDS             complement(<1..167)
                     /locus_tag="OO172_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5511879.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERV"
     gene            320..883
                     /locus_tag="OO172_001655"
     CDS             320..883
                     /locus_tag="OO172_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511880.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(1159..1581)
                     /locus_tag="OO172_001656"
                     /pseudo
     CDS             complement(1159..1581)
                     /locus_tag="OO172_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(1581..2174)
                     /locus_tag="OO172_001657"
     CDS             complement(1581..2174)
                     /locus_tag="OO172_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5511881.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            2321..2728
                     /locus_tag="OO172_001658"
     CDS             2321..2728
                     /locus_tag="OO172_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5511882.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(2848..3840)
                     /gene="rpoS"
                     /locus_tag="OO172_001659"
     CDS             complement(2848..3840)
                     /gene="rpoS"
                     /locus_tag="OO172_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5511883.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(3903..5042)
                     /gene="nlpD"
                     /locus_tag="OO172_001660"
     CDS             complement(3903..5042)
                     /gene="nlpD"
                     /locus_tag="OO172_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5511884.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(5182..5808)
                     /gene="pcm"
                     /locus_tag="OO172_001661"
     CDS             complement(5182..5808)
                     /gene="pcm"
                     /locus_tag="OO172_001661"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5511885.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(5802..6563)
                     /gene="surE"
                     /locus_tag="OO172_001662"
     CDS             complement(5802..6563)
                     /gene="surE"
                     /locus_tag="OO172_001662"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5511886.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(6544..7593)
                     /gene="truD"
                     /locus_tag="OO172_001663"
     CDS             complement(6544..7593)
                     /gene="truD"
                     /locus_tag="OO172_001663"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5511887.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICAKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(7590..8069)
                     /gene="ispF"
                     /locus_tag="OO172_001664"
     CDS             complement(7590..8069)
                     /gene="ispF"
                     /locus_tag="OO172_001664"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5511888.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(8069..8779)
                     /gene="ispD"
                     /locus_tag="OO172_001665"
     CDS             complement(8069..8779)
                     /gene="ispD"
                     /locus_tag="OO172_001665"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5511889.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(8798..9109)
                     /gene="ftsB"
                     /locus_tag="OO172_001666"
     CDS             complement(8798..9109)
                     /gene="ftsB"
                     /locus_tag="OO172_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5511890.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(9303..9626)
                     /locus_tag="OO172_001667"
     CDS             complement(9303..9626)
                     /locus_tag="OO172_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5511891.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(9676..10281)
                     /gene="cysC"
                     /locus_tag="OO172_001668"
     CDS             complement(9676..10281)
                     /gene="cysC"
                     /locus_tag="OO172_001668"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5511892.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(10281..11708)
                     /gene="cysN"
                     /locus_tag="OO172_001669"
     CDS             complement(10281..11708)
                     /gene="cysN"
                     /locus_tag="OO172_001669"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5511893.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(11710..12618)
                     /gene="cysD"
                     /locus_tag="OO172_001670"
     CDS             complement(11710..12618)
                     /gene="cysD"
                     /locus_tag="OO172_001670"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5511894.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            12870..13907
                     /gene="iap"
                     /locus_tag="OO172_001671"
     CDS             12870..13907
                     /gene="iap"
                     /locus_tag="OO172_001671"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5511895.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(14053..15144)
                     /locus_tag="OO172_001672"
                     /pseudo
     CDS             complement(14053..15144)
                     /locus_tag="OO172_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(15338..15490)
                     /locus_tag="OO172_001673"
     CDS             complement(15338..15490)
                     /locus_tag="OO172_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5511896.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(15754..16488)
                     /gene="cysH"
                     /locus_tag="OO172_001674"
     CDS             complement(15754..16488)
                     /gene="cysH"
                     /locus_tag="OO172_001674"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5511897.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMVEEETRFFGLKRECGLHEG"
     gene            complement(16562..18274)
                     /gene="cysI"
                     /locus_tag="OO172_001675"
     CDS             complement(16562..18274)
                     /gene="cysI"
                     /locus_tag="OO172_001675"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5511898.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(18274..20073)
                     /gene="cysJ"
                     /locus_tag="OO172_001676"
     CDS             complement(18274..20073)
                     /gene="cysJ"
                     /locus_tag="OO172_001676"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5511899.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            20392..20754
                     /gene="queD"
                     /locus_tag="OO172_001677"
     CDS             20392..20754
                     /gene="queD"
                     /locus_tag="OO172_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5511900.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            20832..22103
                     /locus_tag="OO172_001678"
     CDS             20832..22103
                     /locus_tag="OO172_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5511901.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            22094..22354
                     /locus_tag="OO172_001679"
     CDS             22094..22354
                     /locus_tag="OO172_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5511902.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            22371..22946
                     /locus_tag="OO172_001680"
     CDS             22371..22946
                     /locus_tag="OO172_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR5511903.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(23094..23954)
                     /locus_tag="OO172_001681"
     CDS             complement(23094..23954)
                     /locus_tag="OO172_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5511904.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIDVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(23951..24730)
                     /locus_tag="OO172_001682"
     CDS             complement(23951..24730)
                     /locus_tag="OO172_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5511905.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTLVERFTLDALFI
                     TLEQRTEHGLRCSRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(24708..26117)
                     /locus_tag="OO172_001683"
     CDS             complement(24708..26117)
                     /locus_tag="OO172_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5511906.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMSGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SIFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(26139..27593)
                     /locus_tag="OO172_001684"
                     /pseudo
     CDS             complement(26139..27593)
                     /locus_tag="OO172_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(27663..28449)
                     /locus_tag="OO172_001685"
                     /pseudo
     CDS             complement(27663..28449)
                     /locus_tag="OO172_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            28768..30045
                     /locus_tag="OO172_001686"
     CDS             28768..30045
                     /locus_tag="OO172_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5511907.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            30072..31542
                     /locus_tag="OO172_001687"
                     /pseudo
     CDS             30072..31542
                     /locus_tag="OO172_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         7801 a         8429 c         7890 g         7880 t
ORIGIN      
        1 acgcgttcgt attttttgtt tggtgtgcga actaccgctt tcagtgaatc ctgagcataa
       61 ccagcatgga gtgaaagctc ccggaaggaa gaccacccgt tatctttgag cgccagctga
      121 atcttcagcg aaggccaatc gcggtcacta tcttctcttt gtctcatctt tcctttaacc
      181 tacaaaataa cccgcgcgga tatccgtatg gataaagatt aagataccaa agattcccta
      241 taaagggttc tttggtgctt ttatggttta ttttttaatt tcattattta tcaatgaatt
      301 agatagaaag gaaccaagaa tgagaaaagc accccaaggt tcttttcctg ccgatggaaa
      361 agaaccgatt atcgaaagaa tctttaggtt agtagaacga tacccttcaa gaaatgcggc
      421 tgctcgggca tgggggatca atgaaggcac tttaaaaaat tattacaaca ggcgggatta
      481 cgcaccgata ccacgcccgc atcagttaaa aaagatagca gaaagcgaaa atgttacctt
      541 ggaatggctc atgacgggag agggtgatgt tgaactaaaa gattccaaag aacccaaaaa
      601 agagaatgcg gataatgttg atgctcaaat tctaatgttc ttgtcatttc ttaaaccaca
      661 agaaaaacaa caagtagctg atgttcttgg gagaaaagga gcggagcaat tactcattct
      721 tctggatgag cacatccaag agttgcacgc tctagttgga gttcgtagag ccatagcgtt
      781 aagccttggt aatttgccag acgaaaaggt tagagagatt tacgcgttgt ccgaagctaa
      841 gggcgatcac ttaaatctac ctcagaaagc agccagcgca taacccagtg caagtgccta
      901 gctgtgctaa aattacattg gttttggcgc ttgcacccat cagcgctata ataaacacct
      961 gaatcgtttt tgaacggtct ttaaaggcta ttgtaagaac ctgtgagtta tcttaaatgt
     1021 tttattgata tcgaaccgtt taacattttt ggactcgctt tatcttgaaa accttgagtt
     1081 cctaacttgt tgattccaat ctattcccgt cttttttaat ctcttcccgg ttatcttgaa
     1141 taaattacct agtcatacga gatagaaacc gccatcgcca tcattcaggc gaaacagcgg
     1201 cagaatatcg aacaggttga tctcttcgcc atcgacggtg ttctgcgccc aggctggatt
     1261 ggcgcggcgc tccggggcaa tcgggaagtt atcccagcgg cggataaact catcaatctg
     1321 ctttttgacc ggggtgtttg gtggcaggcc aagggaaatc gcgtggttct gccaggaacc
     1381 gatggtattc atcgccacgc gggcatcggt aaagccgcga atattatcaa accacagcgc
     1441 gggcgcgccg tcgccgatac gcccggtggc gttagctgct gctgccagat ccggctcagc
     1501 gttcacttct tcgctgattt tcagtaactg gccgtggtca tcaagcgcct gtaaaaagct
     1561 gcgtaaatca tcaaatgcca ttattcattc tcctgagaaa aattccgggc ctgcggcaat
     1621 ccttgccagc gcctggcgtg gggatgttcg aggccaaatt gatccagcac gcgggctacc
     1681 acatggtgga caatgtcatc taccgtttcg ggatggttat aaaaggcagg catcggcggc
     1741 accatcgcca cgcccatgcg tgaaagtgcg agcatatttt cgagatggat ggtgctaagc
     1801 ggcatttcac gcggcaccag caccagtttg cggccttctt tgagcacgac gtccgccgcg
     1861 cgccctacca ggccatcggc gtaaccagcg cggataccgg cgagcgtttt catactgcac
     1921 ggaataacga tcatgccgtc agtacgaaaa gaaccggatg agatgatcgc cgcctgatcc
     1981 gccgggttat ggctgaagtc tgcgagggca gcaacatcgc gagcgctgta aggcgtttct
     2041 agttcaatga tggttttcgc ccacttcgac atcaccagat gagtctcgac attcggcatc
     2101 tcacgcagcg cttgcagtaa tgccacacca agaggcgcac cggtagcccc tgtcatcccg
     2161 acgatcagtt tcattgctta ttctcttaat tgttcgtata cgaacattat tattaacata
     2221 ctccttcatt tccacagcgg caaggattga aaagtaacaa catcttcttc gtcactaaaa
     2281 aaccgctatg atagcgacag agaattagcc cggagtttac atggcgttac gaaataaagc
     2341 gtttcatcag ttacgacagc tttttcagca gcacacggct cgctggcagc acgagttacc
     2401 tgacctgacc aaaccacagt acgcggtgat gcgcgccatt gctgataaac ctggtattga
     2461 acaggtggca ctgatagaag cagcagtcag caccaaagca acgctagcag aaatgttggc
     2521 aagaatggaa aatcgtggct tagtcagacg tgaacatgac gtcgctgaca agcggcgacg
     2581 ctttgtctgg ctgactgccg aaggagaaaa gctacttgct gcggcgatac ctattggtga
     2641 cagcgtggat gaggaatttt tggggcgttt gagtggagaa gagcaggagc tgtttatgca
     2701 gttggtgcgc aagatgatga gcacatagaa tgctgattgc cgggtaggac gctgacgtgt
     2761 cttatccagg cgacaaaaac acaagcttcc caaacaaaag aaaaaggcca gcctcgcttg
     2821 agactggcct ttctgacaga tgcttactta ctcgcggaac agcgcttcga tattcagccc
     2881 ctgcgtttgc aggatttcgc gcaaacggcg caggccttca acctgaatct ggcgaacacg
     2941 ttcacgggtg aggccaattt cacgacctac atcttccagt gttgccgctt cgtaccccag
     3001 caaaccgaat cgacgtgcca gtacttcacg ctgtttggcg ttcagctcga acagccattt
     3061 gacgatgctc tgcttcatat cgtcatcttg cgtggtatct tccggaccgt tctctttttc
     3121 atcggccagg atgtccagca acgctttttc ggaatcacca cccagcgggg tgtctaccga
     3181 ggtaatgcgc tcgttaagac gaagcatacg gctgacgtca tcaactggct tatccagttg
     3241 ctctgcgatc tcttccgcac ttggttcatg gtccagctta tgggacaact cacgtgcggt
     3301 tcgcaggtaa acgttcagct cctttacgat gtgaatcggc aaacgaatag tacgggtttg
     3361 gttcataatc gcccgttcaa tcgtctggcg aatccaccag gttgcgtatg ttgagaagcg
     3421 gaaaccacgt tccgggtcaa acttctctac cgcgcggatc agccccaagt tgccctcttc
     3481 gataaggtcc agcaacgcca gaccacgatt gccataacgg cgggcaattt ttaccaccag
     3541 acgcaagtta ctctcgatca tccggcggcg agaggcgaca tctccacgca gtgcgcgacg
     3601 cgcaaaataa acttcttctt cggccgttaa cagtggtgaa taaccaatct caccaaggta
     3661 aagctgagtc gcgtccaaca cacgctgtgt ggctccctgc gataacagtt cctcttcggc
     3721 caaatcgtta tcactgggtt cctcttctac taaggccttt tcgtcaaaaa cctcaactcc
     3781 gttctcatca aattccgcat cttcatttaa atcatgaact ttcagcgtat tctgactcat
     3841 aaggtggctc ctacccgtga tcccttgacg gaacattcaa gcaaaagcct ggttccgccg
     3901 atttatcgct gcggcaaata acgcagcggg tttacggatt tccccttgta acgaatttca
     3961 aaatgcaagc gtgttgaact ggttccggtg ctacccatgg ttgctatttt ttgccccgcc
     4021 ttcacttctt gttgttcccg gaccagcatt gtgtcgttat gggcgtaggc actcaggtaa
     4081 tcatcattat gtttgatgat aatcagatta ccgtagccgc gcagcgcgtt accagcataa
     4141 acaacgcggc catctgcggt cgcgataatt gcctgtcctt tgctgcctgc gatatcaatc
     4201 cccttgttgc ccccctcaga agcgccaaag gtttcgatca ctttgccctc agtcggccag
     4261 cgccaggtgg agataggcgt actggttgat gtactgctga cagtcggctc ggttgtgctt
     4321 gctgttggta ccgttacagg cgctgtgacc gtggtcgcag ttggcttgtt gttcggcaac
     4381 attttgttag cactctgttc acccgaagac tcagaatacg taattgtcgg ttgcgacgca
     4441 acagcaacgg tggaattttg tgcaggcttg atcacaactc cttgctctgc tgcgtcggcc
     4501 tgggtaatag catttccgcc agtgattggc gtaccggaag cattacccac ctgcaaggtc
     4561 tgaccaacgt tcagcgcgta tggtgcctga atattgttgc gctgagcaag gtcacggaaa
     4621 tcgttgccag taatccaggc gatatagaaa agtgtgtcgc cttttttcac ggtataggta
     4681 ctgccgctat aactgccttt cggaatgttc ccatactgac ggttatagac gatgcgtccg
     4741 ttttccatct gtaccggctg ctgagctact ggctgcactg gctggatttg cggttgttga
     4801 gtagcctgaa tttgtggctg ctgtaccggc tgaatttgcg gttgctgcgc tgtagacgtc
     4861 gtccctattt tcggcggcgg cgtaatcaac ataccagaat tagtatttgc aggcgcattg
     4921 ccattaacgg agctgaccgg tgccggtgga tttgaagtgt cagaacagcc tgccagccat
     4981 agcgaaacca gtgacaaagc cgcaatgcgg cgaacggtga attttgggct tcccgcgctc
     5041 atttatcccc caggaaaaat tggttaataa ccagtgacat aattaccgtg caaggcaccc
     5101 tactgaacac tggaaaagat gttcacgata cgctgaccta cggcaaaata accaggaaaa
     5161 atccaggtat ttcctcacgt tttaagccag ctcacccttc actaaaggga caaagcgcac
     5221 ggcctccacg gtatcgataa taaattcgcc tccccgacga cgcacccgtt tcaaatactg
     5281 gtgctcctcc cctacgggta agacgagaat cccgccttcg tccagctgcg tcattagcgc
     5341 agttggaatt tccggcggtg ccgccgtaac aatgatagcg tcaaacggcg cacgtgcctg
     5401 ccaaccttgc catccatcac catgacgggt tgaaacatta tgtaaatcaa gatttttcag
     5461 gcggcgacgt gcctgccact gcaagccttt aatccgttca accgagcaaa catgctggac
     5521 aagatgcgcc aggattgccg tttgatatcc cgaaccagtg ccaatttcca gcacccgcga
     5581 ctgcggcgtc agctcgagta attcggtcat tcgcgccacc atatacggct gcgaaattgt
     5641 ctgcccctga cctatcggca aggcgatatt gtcccaggct ttttgttcaa acgcttcatc
     5701 aacgaatttt tcacgcggca cggcggcaag tgcattcagc acctgctcat cctgaatacc
     5761 ttgcgcacgt aattgatcca gaagtgcttg tacgcgtctg cttaccattg cgtgccaact
     5821 cccacgctgt ttaaccagtc tgaaaccaca tcttgcgcgc tatgcgcagt taaatccaca
     5881 tgcagcggcg tgatggagac atagccctca tctaccgcag caaaatcggt ccccggaccg
     5941 gcatcacatt taccgcccgg cgggccaatc cagtacagcg tattgccgcg cggatcttgc
     6001 tgcgggatca cctgatctgc cggatgtcgt gtaccgcagc gcgtcacgcg aatacctttg
     6061 atttgatcca agggtaaatc cggaacgtta atattaagaa tacgtccggt gcgcagcggc
     6121 tctttacaca gtgcgcgcaa aattgaacag gttaccgccg cggcagtgtc gtaatgttta
     6181 tgcccgtcaa gcgagacggc cagcgccgga aaacctaaat gacggccttc catcgcggcg
     6241 gctaccgtac cggaataaat aacatcatcc cccagattcg gcccggcgtt aattccggac
     6301 acaacaatgt ccgggcgcgg acgcatcaga gcattcacgc caagatagac acaatcggtc
     6361 ggggttccca tttgcacagc aatatcacca ttttcaaagg taaacgtgcg cagggaggat
     6421 tccagtgtca gagaatttga agcgctgctg cggttacgat cgggggcgac cacctgaacg
     6481 tcagcaaact cacgcaaggc tttcgccagc gtttgtatac cgggtgcatg taccccgtca
     6541 tcattactca gcaatatgcg cataatcacc tgttgtgttg ataagttccc tgacaacgct
     6601 ggttgcaaaa ctacccgccg gaagccagaa acggatctct acggtgacgt catcccacca
     6661 gttccagctt aattgttgcg gatacagcag catcgctctg cgcgcggctt caactttttc
     6721 gcgcaccagt aaagcttgta attcagtttc tgcggcgaca gctgcttgtt cgaatgccag
     6781 cgcttcacgc tgagttcccc attcgccact gcccggcaat gcggcggtta tcatcagctc
     6841 tttatcgttg acgcgacgct gtaattccgc cagttcttcg gtggttgcta caaaccagct
     6901 accacgtccg gctaattgta gcgcatcgcc gtcaacaact tgattaacgt ctgctttttt
     6961 gaggcgctca gcaacaatct gattaaacaa cgcactgcgg gctgccgaca accaaaaact
     7021 ccgtttattg cgatcgcgca ccggagtatt ggtttgcgcc cagcgcagcg cgccctgcaa
     7081 gttgctaccg ccaatcccaa aacgttgggc accgaagtag ttcggtacac cttttgcgca
     7141 aatatcgatc agacgttgtt caacgtcatc gcgattgctc acttcgcgca gaaccagggt
     7201 aaaggcgtta cctttcagcg cccctaaacg cagcttgcgc ttgtgccgcg catactccag
     7261 cacctggcag ccttccagtt gaaaggcgct cagatcgggc atttccttgc ccggcacgcg
     7321 agcacataac cactgttccg ttacagcatg tttgtctttt tgcccagcga agctgacttc
     7381 acgggaatga attttcagga atttcgccag tgcatccgcc acaaaacggg tattgcagcc
     7441 gtttttgagg attctaacca gaatatgctc accttcacca tcaggctcaa agcccaaatc
     7501 ttccaccacc acaaagtctt ccggattggc tttcagcagc ccggtgcctt gcggtttacc
     7561 gtggaggtaa gtgagattat caaactcaat cattttgttg ccttaatgag tagcgccacc
     7621 gcttcacagg caatcccttc cccacgtccg gtaaatccaa gtttttccgt agtagtggct
     7681 ttcacgttaa catcatccat atggcagccg agatcttcgg caataaacac gcgcatttgt
     7741 ggaatgtgcg gcaacatctt cggtgcctga gcgatgatag tgacatcgac gttgccaagg
     7801 gtataaccct tcgcctgaat acgacgccag gcttcgcgta gcagctcgcg gctgtcggca
     7861 cctttaaatg ccggatcggt atccgggaac agtttgccga tatcccccag cgccgccgcg
     7921 ccaagcaatg catcggtcaa cgcatgaagc gccacgtcgc catcagaatg cgccagcaat
     7981 cctttttcgt aaggaatgcg tacgccacca atgataattg ggccttcacc gccaaaggcg
     8041 tgtacgtcaa aaccgtgtcc aattcgcatt atgtattctc ctgatggatg gttcgggtga
     8101 ggtaaaactc ggccagtgcc aaatcttccg ggcgcgtgac tttaatgtta tccgcgcggc
     8161 cttcgaccaa ctgcggatga aatccgcaat attccagcgc cgaggcttcg tcggtaatgg
     8221 tagcaccttc atttagagcg cgcgtcagac agtcatgtaa cagctcacga gggaaaaatt
     8281 gcggcgtcag cgcgtgccat aagccgttgc gatcaacggt atgagcaatg gcatttttgc
     8341 ccggttcggc acgtttcata gtatcgcgca ctggtgcggc gaggatccct cccgtgcggc
     8401 tggtttcgct caacgccaac aatcgcgcga ggtcatcctg atgcaaacaa ggacgagcgg
     8461 cgtcatgcac caatacccac tgcacatcgc cagcggcttt cagacctgcc agcacggaat
     8521 cggcacgctc atcaccgcca tctacaacgg tgatttgcgg atgattcgcc agaggaagtt
     8581 gtgcaaaacg gctatcgcca ggacttatgg caatgacgac acgtgtcacc cggggatgcg
     8641 ccagcagcgc atgcaccgag tgttcaagaa tggtttgatt accgattgag agatattgct
     8701 taggacattc cgtttgcatt cgacggccaa atccggccgc cggaaccacg gcgcaaacat
     8761 ccaaatgagt ggttgccatg ttaattcccg ggctgattta tcgattgttt tgccccgcag
     8821 actgtgcgcg cttcgacgcg tcaggcacca gacgataaaa agtttcgccc ggcctggtca
     8881 tgctgagttc attacgcgca cgctcttcga gcgcctcctg gccgccattg agatcgtcaa
     8941 tttcggcaaa aagttgatcg tttcgcgctt taagtttcgc gtttgtagct tgcagtgccg
     9001 ccacatcatc attgacgcgg gtatagtcat gtataccgtt cttaccgaac cacagcgaat
     9061 actgtagcca gaccagaata gccagcaaca gcagcgttag tttacccatc ctgccccctg
     9121 aaaaactgca tcatcatccc atgcatccga agacgactct acatcctctg ttggggatac
     9181 cgcgacaacg cgggcaaatg taccacattt gtccattgtt acgtataccc agggcgtgca
     9241 gaacataatc tcattattag ttacggtttg aattatgaac agaggagaca agaaagtaca
     9301 aattagccca gtagccacat aaacagtgcg ccaaacataa tgcctactgt caccagggtg
     9361 aaaacaatac tgtagcgtag ctttccgtcc atcaatgaat gcagcgcaat ccccaccact
     9421 accgcgacag gcatcagtgc cagaaagaaa ggccaggtgt agataaagaa gaacagcgtg
     9481 ttagagccat aaatcaacat cggcatcgcc agcgcaaata accaggagat aaaaccgacc
     9541 acggcaccag gcagtgacca tgtagtttct tcatcctcag taaggctgtc gttatttgtt
     9601 agtgtaatgt tatggctatt acgcatattt gatcctgtta ctttgacgaa ccgggcattg
     9661 aaacccggtg gtgtctcagg atctgataat atcgttctgt ctcaacagat ctaataattg
     9721 ctgtaccaaa tttgttacta attgttcacc attgagatga atttctgccg attcaggctc
     9781 ttcgtaaacg gaatctattc ccgtaaagtt gcgcagttca ccggcacgcg ctttcttata
     9841 taagcctttt ggatcgcggg cttcgcaaat cgccagcggc gtatcgacaa acacttcaat
     9901 aaagcgccct tctcctacgc gttcgcgaac catctggcgt tcggcgcggt gtggcgagat
     9961 aaatgcggtc agcaccacca gtccggctgc aaccatcaaa ttcgccactt caccgacgcg
    10021 acggatattc tctttacgat cggcatcgct aaaaccgaga tcgctgcata atccgtggcg
    10081 aacattgtcg ccatccagca gatacgtact gacgccgagt ttatgtaacg cctcctccag
    10141 cgccccggcg accgttgatt taccggaccc ggagaggccg gtaaaccaca gcactacacc
    10201 acgatgaccg tggtgtagtt cgcgttgttg cacagtgacc ggatgactat gccagacgac
    10261 gttttcgtca tgcagcgcca ttatttatcc cccagcaaat cgcgcgcgcc ccagtgcgga
    10321 aagtggcgac gaaccagggc attcaattcc agttcgaatg cactgaattc agatgacgca
    10381 gctgttgcct ggctaactgg ctcgtgcacc ataccggcac ccacggtcac attgctcagg
    10441 cgatcgataa aaatcagccc accggtaacc ggattttgct gatagcgatc taacaccagt
    10501 ggctcgtcaa aagtgagatc caccaggccg atgccgttca gcggcaggtt ttcaacttcg
    10561 cgttgggtaa ggttattaat atcaacctga taacgaatgc catcaacacg agcacgcgtc
    10621 ttcttaccgg caattttgat gtcataactc tggcccgggg aaagcggctg ttccgccatc
    10681 cataccacat ccaccgacgc actctgcacc gccggtaacg cttcgtctgc cgccagcagc
    10741 agatcaccac ggctgatatc gatctcatcc gtcagcacca gggtgatcgc ttctccggca
    10801 aagacttctt cgcgatcacc atcaaaagtc acgatccgcg cgacgtttga ttccacacca
    10861 gagggcagca cttttacacg ttgcccgact tccacgcgac cggatgccag cattccggcg
    10921 tagccacgaa aatcgagatt tgggcggtta acgtactgca ccgggaagcg cattggctgg
    10981 gcatccacca ctcgctggat ctccacggtt tccagcactt cgagcagtgt cagaccgctg
    11041 taccacggca tactttcact ttgcgaagcc acgttgtcac cttccagtgc ggagagcggc
    11101 acaaagcgga tatccagatt acccggcagc tgcccggcaa aggtcagata atcttcacga
    11161 atacgggtga acgtctcttc actgtaatcc accagatcca ttttgttgat cgccacgacc
    11221 agatgtttga tccccaacag tgtggagata aaactgtgac gacgggtttg atcgagcacg
    11281 cctttacggg catcaatcag taagatcgcc agttcacatg tcgatgcgcc agtcgccata
    11341 ttgcgagtgt actgctcgtg ccctggggtg tcggcgataa taaatttacg cttctcggta
    11401 gagaaatagc ggtaggccac atcaatagtg atgccctgtt cgcgctcagc ttgcaggccg
    11461 tccaccagca gagccagatc cagcttttcg ccctgggtgc cgtgacgctt actgtcgtta
    11521 tgcagcgatg agagctgatc ttcgtagatt tggcgggtat cgtgcagcag acgaccaatc
    11581 agggtacttt tgccgtcatc gacgctacca caggtcagaa aacgcagcag gcttttatgt
    11641 tgttgcgcaa tcatccaggc ttcgacgccg ccttcattgg cgatttgttg tgcaagtacg
    11701 gtgttcatct taaaaatacc cctgacgttt tttcagctcc atggatcccg cctggtcgcg
    11761 gtcaatcacg cgaccctgac gttcactggt ggtggaaacc agcatctctt cgatgatttc
    11821 cggcagtgtt tgtgcatttg actccaccgc accggtcagc ggccagcagc ccagcgtacg
    11881 gaaacgcacc atccgttttt tgatcacttc gcccggttgc aggtcgatac ggttgtcatc
    11941 aatcatcatc aacataccgt cgcgttccag aaccggacgt tccgcagcga gatacagcgg
    12001 cacaatgtcg atattttcca gccagatgta ttgccagata tcctgctcgg tccagttaga
    12061 gagcgggaag acgcggatgc tttcgccttt gttaatttgc ccgttgtagt tgtgccacag
    12121 ctccgggcgc tgatttttcg gatcccagcg atggaagcgg tcacggaaag agtagattcg
    12181 ctctttagcg cgggatttct cttcgtcacg gcgcgcacca ccgaaggcgg catcaaaacc
    12241 gtatttgttc agcgcctgtt tcaggccttc agttttcata atatcggtat gtttcgcgct
    12301 gccgtgcacg aatggattaa tccccatcgc cacgccttcc gggtttttat gcaccagcag
    12361 ctcgcagccg taggctttcg ccgtacgatc gcggaactca tacatctcgc ggaatttcca
    12421 gccggtatcg acatgcagca acgggaaagg cagcgtacct ggataaaacg ccttgcgcgc
    12481 cagatgcagc atgacgctgg aatctttacc gatagagtag agcatcaccg gatttgagaa
    12541 ttctgccgcc acctcgcgaa taatgtggat gctttccgcc tccagttgcc gcaggtgagt
    12601 aagtcgtttt tgatccataa ccgttccttt gcaataccgc tattttcttg ccatcagatg
    12661 tttcgactat agggagcgta agagaacgaa tgaaattacc aattagaatg agtagttcct
    12721 taacggaata acgatttggc aaagctaata tcaaaaagtg cttaaggcac cggatttcgg
    12781 gcgtttagga agatttgaaa ttgttttagc gcagcggcag tttcatacta tggcggtaaa
    12841 aaaatttgca tggtatttaa ggactcacta tgttttccgc attgcgccac cgtaccgctg
    12901 ccctggcgct cggcgtatgc tttattctcc ccgtacacgc ctcgtcacct aaacctggcg
    12961 attttgctaa tacccaggca cgacatattg ctactttctt tccgggacgc atgaccggaa
    13021 ctccagcaga aatgttatct gccgattata ttcgccaaca gtttcagcaa atgggttatc
    13081 gcagtgatat tcggacattt aatagtcggt atatttatac cgcccgcgat aatcgcaaga
    13141 actggcataa cgtgacagga agtacggtga ttgccgctca tgaaggcaaa gcgccgcagc
    13201 agatcatcat tatggcgcat ctggatactt acgccccgct gagcgatgct gacgcagatg
    13261 ccaatctcgg cggactgacg ttacaaggaa tagatgataa cgccgcaggt ttaggtgtca
    13321 tgctggaact ggcagaacgc ctgaaaaata cgcctaccga gtatggtatt cgatttgtgg
    13381 cgaccagcgg cgaagaggaa ggaaaattag gcgctgagaa tttactcaag cggatgagtg
    13441 acaccgaaaa gaaaaatacg ctgctggtga ttaatctcga taacttaatt gttggcgata
    13501 aattgtattt caacagcggt gtaaaaaccc ctgaagcagt aaggaaatta acgcgcgaca
    13561 gggcgctggc aattgcacgc agtcacggaa tagccgcaac gaccaatccg ggtttgaata
    13621 aaaattatcc gaaaggcact gggtgttgta atgacgcaga aatattcgac aaagcgggca
    13681 ttgctgtact ttcggtggaa gcgactaact ggaatcttgg aaataaggat ggttatcagc
    13741 aacgcgcaaa aacacctgcc ttcccggcgg gaaatagctg gcatgacgta agactggata
    13801 atcaacaaca tattgataag gcacttccag gaagaataga acgtcgctgc cgtgacgtta
    13861 tgcggataat gctaccgttg gtgaaggagc tggcgaaggc gtcttgatgg gtttgaaaat
    13921 gggagctggg agttctaccg caggggcggg gaattctaag tgatacccat catcgcatcc
    13981 agtgcgtccg gtttacttcc gggaaaaccg caccgtattg cgccggttta tctccgctgg
    14041 agcggggaac acctctggcg aatagaaagc aagccaggtt ttgaccagtc gatcgtacat
    14101 ggcattggcc gttgccattg ttggcaggcc aaaaaacagc ccctgagcgt tacccgcagc
    14161 catcaacctg tgcgccagga taagcgccgc ttctgtttta cctgcgccgg tcacatcttc
    14221 cagaataaac aactgtggtc ctggctgatt gatatccaga tccagcacct tttgctgtaa
    14281 cggtgtcgta ttctcaataa aaggaaacag cgtattaatt ccggtgaaag gcgcggtttc
    14341 tgcttgtggc ggaaagacgg ttaatgcgtt ttgagcctga actaaagcct tttgccagta
    14401 atcttcaata tctattgcct gtgcgacgcg tggaaaaaat cgcgttgacg accctgtcca
    14461 gtctgcaagt acgacggttg cagagatata ccaggaaagt tgttttataa gttcaacgcc
    14521 ctcgtcatca tcccagaatg tggggatctc tattcgcgga aacagtggct tgatttcaag
    14581 gagaaaatct cgtgctgcag ctttgtcttc aggcagaaaa ttatccaatt catccatacg
    14641 atcagggggc cgaccatgat gccctgtcgt cacgggcatc cacatttcta tcactcgcct
    14701 aagtttgcgc ggagagagcg aagatgaagg aagtaactcc tgacattcac ttaaataatg
    14761 attccacaac cagtagccca gcgttgaatg agagattttt tcgtaatttt ttctggcacc
    14821 ttcctgaatc ttgaattcag ggggcaggta aagttgttga aaagagcggg caaattttcc
    14881 aatatcgtgc cagcacagca accaggcgaa aaattgagct gcctgttcct tgtcagaaat
    14941 ccctaattga cgaaaataat cagccagccc gaagcaattt cttttaacca ttaaatagcc
    15001 cattgcggcc acatccagca aatgccagca aagaaggtga tagtcgtcgc taccctcttt
    15061 ctcgccacgt cggttttttc cccagaaatc aaagaaagtc acaatatttt tatccttcag
    15121 taaacttaaa ggatatttac gcatatttta aaaactatct gtgatatata tcagccaata
    15181 aaacatttac attatgaata aattaatgat tttcatttga aattcataat aataaataga
    15241 gactatatat tcacataaaa aatataaatt ttaaaaaagg gtgcaggaga ccaaaccatc
    15301 acaattcagt acgcggggtt ttacccccgc gtggtggcta cttcttcgat tcgtaagcga
    15361 gaactgcctt aaactccata ccacgttctc tgatactcaa ctcacataac gagtcaccta
    15421 ccatcaacgt gaatcccagt actgtacaac acagtacgat gattgccaca agggcatatt
    15481 ttgtcagcat gtcttgcctc cctgcaaaga agagactaac atccgaattg ctacgttcga
    15541 acgcgagcct caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg
    15601 aatgcttaac gccagacagc ctcaagcacc caacgccatt ctatacctgt taattttccc
    15661 tgcgatgttc acctttaaat gccggaatgc tgctcgcaaa ataaaaacgc ccccatcatt
    15721 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgtaatc cacattccct
    15781 ttttaagcca aagaaacgcg tttcttcttc caccatgccg ggttcccatt tacgggttgt
    15841 atgggtatcg cccaccgata aatatccttc gtcccataat gggtgatatt tcaggccatg
    15901 tttttgcagg tactgataaa tagttcggtt atcccagtcg ataatcggca gcactttaaa
    15961 tacgccacgc tgaattgcca gcaccggtaa attggcacgg ctgccggatt gttcacgccg
    16021 caggccagca aaccacgttt gcgcattcag ttctttcagc gcccggttca tcggttcgac
    16081 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgttcccaca gtttgccgta
    16141 gcgtgcttcc tgccaggcgg cgctttcggt agcgcggtac actttcaggt tgagcttgag
    16201 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacagatagc cggtgtcggt
    16261 gaggatcacc ggaatatccg ggtgaatctg attcaccaga tgcaggctca ccgccgcctg
    16321 aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggcaac
    16381 gcggccttca gcgtccagtt tttccagttc ggcgttagtt tctgccagcg ccagaatgcg
    16441 atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact
    16501 gtttgccttg cctgatgcga cgctcacgcg tcttatcagg cctacaagac cgggctgatg
    16561 gttaatccca caaatcgcgc gccggatcga gcaccgggcg aatgatgccc gcacgcaccg
    16621 taaagtcgcc gaagccttca cccgcttcgc gctctttcgc ccagcgccct atcagttcat
    16681 caagcgacgc caggatttcc ggctcggtga tgttttcttt atacatccgt gggatacgtg
    16741 tcccaatgcg gttgccgcca agatgcaggt tgtagcgacc cggtgcttta cccaccaggc
    16801 ccacttccgc cagcatcgcg cgaccacaac cgttcgggca gcctgttaca cgcatcacga
    16861 tatgctcatc gctgacacca tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg
    16921 gcaggaaacg ctccgcttcc gccatcgcca gcgggcaagt cgggaatgac acgcaggcca
    16981 tcgagttttc acgctgcggc gtgacggcat tcattaaccc gctctctttg gcaattttct
    17041 cgatcttcgc tttctcgctt tccggcacac cggcgatgat cagattctgg ttcgccgtaa
    17101 tgcggaaatc acctttgtgg atcttcgcga tctccagcag gccagttttc agcggacgcc
    17161 ccggataatc aaggatgcga ccattttcga taaacagcgt caggtgccag ttatcatcaa
    17221 tgcccttaac ccagccaata cgatcgccgc gtcctgtaaa ttcatacggg cggatcggtt
    17281 caaatttgat ccccgcgcga cgctccactt ccgctttaaa cgtctcaacc cccacgcgct
    17341 ccagtgtgta ttttgttttg gcatttttac gatcggttcg gttaccccag tcacgctgag
    17401 ttgtcacgac ggcttccgcc accgctagcg tatgctctag cggcagatag ccaaactcac
    17461 tcgccgtgcg ggcgtaggtt ttcttgttac cgtgttcgat agaaagccca ccgcccacca
    17521 gcaggttgaa gcccaccagc ttgccgtttt ccgcgatcgc cacgaagttc atgtcgttgg
    17581 cgtgcagatc aatatcgttc tgcggcggga tcactaccgt ggttttgaac ttacgcggca
    17641 ggtaggtctg accaaggatc ggttcttcat cggtggtggc gactttttcc tgatcgagcc
    17701 agatctccgc atacgcgcgg gtacgcggca gcagatgttc agaaatcttc ttcgcccact
    17761 cgtacgcttc tgcgtgcagc tgcgactcgt aagggttcga ggtgcagagt acgttacggt
    17821 tcatgtcgtt agcagtcgcc agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg
    17881 gtttgacgtt ctttttcaga atgccgtgga actgaaacgt ctggcggttg gtcaggcgaa
    17941 tgctgccata gatggtgttt tcaccggcaa atttatcgat cgcctgccac tgtttggtgg
    18001 tgataactcc acccggcaga cggcagcgca acagcatcgc gtggcgcggc tccagcttct
    18061 gttcagcacg ttcggcgcgg atgtcgcggt catcctgctg atacatgccg tggaagcgga
    18121 tcagcaggaa gttatcgccc ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa
    18181 tggtgccgcg caggtagttg ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt
    18241 cgaccactaa aggccctgga tgtttttcgc tcattagtag acatctcgct gataacggcg
    18301 ctctacgcgc agctcactta aaaattcatc cgccgcttcg gtgtccatgc caccaaattc
    18361 ggcaatcact tccagaagtg cctgctcaac gtctttcgcc atgcgattag cgtcgccgca
    18421 gacataaatg tgggcaccgt cattgatcca gcgccacagc tccgcgccct gttcgcgcag
    18481 tttatcttgt acgtaaactt tttctttttg gtcgcgcgac caggcgagat cgatccgcgt
    18541 cagcacaccc tctttgacgt agcgctgcca ctccacctga tacaggaagt cttccgtaaa
    18601 atgcggattg ccaaagaaca gccagttttt acctggcgct tcgtcagcgg cgcgttgctg
    18661 cataaaggcg cggaacggcg caataccggt gcctgggcca atcataatca ccggggtttc
    18721 caggttagcg ggcaggcgga agttatcgtt atgttcgata aatacgcgga cttcgccctc
    18781 ttcttccacg cggtcagcga ggaagctgga ggcaccaccg gcacgggcgc ggccttccac
    18841 gtcgtaacgc accacaccaa cggtgacgtg tacttcgttc tcgacttccg cctgcgagga
    18901 ggcaatggaa tagagacgcg gggtcagcgg gcgcagcaga ttaattagcg cttcggcatc
    18961 aagctgtgcc ggggagaaac gcaccatgtc aacaatcagc gtcgtcgcgg cgtaatgctg
    19021 taacttcgct ttatcgccca ccagcggcag cagtgtttca ctgcgggtaa gcgtggcgta
    19081 attctcaaca atgttggcgg tgttgacggt cagttcgaag tgccactgta gcgcttcgtt
    19141 cagaggcaac gttttgccct cgacagtgac aggttcatcg cctgtcagcc acagcagttc
    19201 gacaagttct ttcaccagtg ccggatcgtt ctgataccag acgcccagcg cgtcacccgg
    19261 ctggtaacgc aggcccgagt cgcctaagtc aatttcgata tggcgaacgt ctttttcaga
    19321 gttacgccca gtaattttct ggttaacaga gaggctagcc accagcggcg cgtctttgct
    19381 gtacgggctg gtgtggattt catttaccgc gccagtagcg acggattgcg aaggtgccgc
    19441 gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc
    19501 ctggtattca acatcagaat cgacacggtc gagcaggcgt tcaccaccca gttccgccag
    19561 cttgctgtca aaatctttcc cggactggca gaaaaattca taagagctat cgccgaggct
    19621 aaacacggca aacgcggtgt tttccagctt tggcgctttt ttggagaaca ggaacttatg
    19681 cagcgcgacg gcttcttccg gcggttcccc ttccccttgc gttgacgtca ctacgatgag
    19741 cagtttttcg ctggcgattt gtttgaattt atagtcgccc gcgttcacca gcttaacgtt
    19801 cagttttgct gctaataaat catcgcgtaa tgcttcagca acccggcgcg cattgccggt
    19861 ttgcgaagca gagataatcg ttatacccgg catttctgcg gcgggcgctg gcgtcactgc
    19921 aagcgcaaca ggctgctggt tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg
    19981 ggtaggcgtt aaatcggtcg tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg
    20041 aagcaacgcg gaaggtggga cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc
    20101 tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa ggtaaggtta acggggtgaa
    20161 cggtgtggat taaagacggg atagcgataa ctaataacca aatcgactaa cctgttttag
    20221 caatagtctt taacaacaaa atagattaac caacctaatg aaaaacaaat gaatttagcc
    20281 aatcattaag ataaatcagc gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa
    20341 cagcctttcc ggtactctac ggcggtttta tcgtcctgta gagaaattat gatgtccacc
    20401 acgttattta aagatttcac cttcgaagcc gctcaccgct taccacatgt cccggaaggg
    20461 cataaatgtg gtcgcctgca cgggcattcc tttatggtgc gactggaaat taccggggaa
    20521 gtcgatccgc atacgggctg gattatcgat ttcgctgaac tgaaagcggc gtttaagcca
    20581 acttatgagc gcctagatca ccattatctc aatgatattc caggtctgga aaacccaacc
    20641 agcgaagttt tagcaaaatg gatttgggat caggttaaac ccgttgtgcc gctgttaagt
    20701 gcggtgatgg taaaagaaac ctgcaccgca gggtgtatct atcgcggcga atgataagag
    20761 tgtgtcggcg gtcaatttcc ctttagtaac gctatgttag ggtgttgtgt tctggatatc
    20821 tggggcatga catggaagac gactgcgaca ttattattat tggtgccggt attgcaggca
    20881 ccgcttgcgc gttacgctgc gcgcgagcgg atttatccgt tttgttactc gaacgcgctg
    20941 aaatccccgg cagcaaaaat ctttccggca ggcggttata tacccatgca ctcgcggaac
    21001 tcctcccgca atttcatctg accgcgcctc ttgaacgacg catcactcac gaaagccttt
    21061 ccctgttaac gccggatggc gcaacgacgt tttccagctt acagcccggc ggtgaatcct
    21121 ggagtgtatt acgtgcacgg ttcgatccgt ggctggttgc cgaagccgaa aaagaaggtg
    21181 tcgaatgcat ccccggagcg acggtggatg cactgtatga agaaaacggc agagtctgtg
    21241 gcgttatttg tggtgacgat attctccgcg cccgttatgt ggtgctggca gaaggtgcca
    21301 acagcgtcct ggctgaacgt cacgggttag tgactcgtcc tgctggcgaa gcgatggcgt
    21361 tggggatcaa agaagtgctg tcgctggaaa catccgctat tgaagaacgt tttcatctgg
    21421 agaataacga aggcgcagcg ttgctgttca gcggcggaat ctgtgatgac ttacccggcg
    21481 gcgcatttct ttatactaat caacaaacgc tctcgttagg gattgtttgc ccgctctctt
    21541 cccttacgca aagtcgtgtt ccggcaagcg agctgctgac tcgctttaaa gcgcatccgg
    21601 cagtgcgcac gcttatcaaa aacacggaat cactggagta tggtgcgcat ctggtgccag
    21661 aaggtggctt gcacagtatg ccggtgcaat acgccggtaa cggctggctg ctggtgggcg
    21721 atgcgttgcg cagttgcgtc aataccggaa tttccgtgcg cggcatggat atggcgctga
    21781 ctggcgcaca ggcggcggca caaacgctaa taagcgcctg ccagcaccgc gagccgcaaa
    21841 atctgtttgc gctttatcat cacaacgtag agcgcagcct gctatgggat gttctacaac
    21901 gttatcagca tgttccggcg cttttgcaac gccctggctg gtatcgggcg tggcctgcgt
    21961 taatgcagga tatttcccgc gatttatggg atcagggtga taaacctgtt ccaccgctgc
    22021 gccagttatt ctggcgtcat ttacgtcgtc atggcctgtg gcatctggcg ggcgatgtta
    22081 tcaggagtct gcgatgtctg tagcccgtaa tctctggcgc gttgctgatg cgccgcacat
    22141 tgttccggct gactccgttg agcgtcagat ggtacaacgg ttgattaacg cctgtccggt
    22201 aggtcttttt tctctcacac aggaaggtgg cttacgtatt gactatcgcg gctgcctgga
    22261 gtgtggcacc tgccgtttgc tgtgcgacga atccacacta caacagtggc gctatccgcc
    22321 ttccggattc ggcatcacct accgctttgg ataaataata aggataattt atgcccctct
    22381 tacacctgct ccgccagaat ccggtgattg ctgccgttaa agataatgcc agcctgcaac
    22441 tggccatcga ttctgaatgc cagtttattt ccgtgttgta cggcaatatc tgcaccatca
    22501 gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc ttttattcat gttgatttgc
    22561 tggaaggcgc gtcaaataaa gaagtggtga ttcagttttt gaaactggtg accgaggcgg
    22621 atggaattat cagcaccaaa gcatcgatgc taaaagcggc aagagcggaa gtttttttct
    22681 gtattcatcg cctgtttatt gttgattcga tttcgtttca caacattgat aagcaagttg
    22741 cgcaatcgaa tccggattgt attgagatcc tgccaggctg catgcccaaa gtgctgggct
    22801 gggtgacgga aaaaatccgc caaccgctga ttgctggcgg gctggtatgc gatgaagaag
    22861 atgcgcgtaa tgcgattaac gcgggtgtcg tggcgctttc caccacgaat accggagtct
    22921 ggacgttagc gaaaaaattg ctttgacggg ataatcgtaa ccaattgaat ttgttttgat
    22981 ttgttggccg cacgccacat ccgacattca gcgcctgatg cgacgcttaa cgcgtcttat
    23041 caggcctaca ggtccccagt ccagaaccgt aggcaggatc atccggcagc acgttattgg
    23101 tattctgcgt gaatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg
    23161 tcaatatctg cctgtgaaaa taccgctgcg ctggcgtcat gattaatcgc caccataaac
    23221 ttgctattgc gtacgcccgc cattaacgct gctgcgccgg aagcgccaac aacaatgcac
    23281 acctcaggtg ccagcagatg tccggaaata ccaatcactt tttcagcatc aacgccaccg
    23341 ttcatcactc gcgcccgact gtagcccact tcggttccca gcttttctgc cagcataacg
    23401 atttcctggt tatctgcctc tccgccctgc cccaccacca gcacccgtct ggcctcagct
    23461 aatgggtcgc ggctaacatt tttcagatcc tctgtgctaa ccagccagtc gggaagcgca
    23521 cctgggacaa tgttcagttg ctgcattcca gagggcaacg tggcgttttt cgctgctccc
    23581 gcctgacgcg ccagggaaag gcataacggg cgtttttctg tttgcagcgt cgccgttaac
    23641 gcatttcccc agtgggattt gcgcatgctt acggttggaa tatctaacga agtcacctga
    23701 cagatgcttg caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgtt
    23761 cccggcggaa agagaacaac gtccgcaggc gttcgctgcc actgttccac catcgcatcc
    23821 agcacctgtt ccgccactac gggctgaggt tctatttgcc agtgcgccag cgtacaaccg
    23881 ctgaaatcct gcgccgccag ccagctcgct attgcggcat tctcctgatt tattgtcaca
    23941 attgcgatat tcatggctgc atcctttggc gtaaatagtc ctgccatagc ttttgagctt
    24001 tttctgctac cgtttgccca tcaatcagcg tcgctccgca tcgttgctct gctcttgcca
    24061 gttgcagaca ctgcatcgct ggcatttcgg cagcaacagt tttacggata atttctgctt
    24121 tccccgcagc cattcgctgg cgcattcccg gtactggcaa ggccacttca ccgcactgac
    24181 gcacggcaat caccgcaggc aagcgaaccc tcgaacagcg caacccatgt tcagtacgct
    24241 gttcgagggt gataaacagc gcgtcgagag tgaaacgctc caccagggtg aagcagggcc
    24301 agcccagcat ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc
    24361 agccggtaat tatcagatcc agcggattct gacgctgcca ttcggcaata tggcgagcga
    24421 caaattccgg cgcaaagcgc agatctgccg ccgtctccag caatacagct tcctcaaacc
    24481 caagagccat gagatagcgc agccagtgca acgcccgttc atcccccatg ctcaacgcgg
    24541 ttaaagacat cggcgtaccg ttttttctct gcgctaacag cagcgcggca gcggcctgtt
    24601 catcagcacc gagtaaactt cgcagtagcg aaacatccgg tccgctatta ccctgagccg
    24661 ccgcctgcca ctctttttcc gccagcattc cggcatccgg ttcggcttta aacgctaaca
    24721 gaatgttcat tcgcacctcc tacatttccc gccgccacca gcgggagggc tttagtttct
    24781 ggtgcccata gccaggtcac aacaaaacca accaacaaca ctgtcgccag gagcaataag
    24841 gtgacttgca ttccccactg cgccagcacc cacggcagca ggccagtact taccgccgcg
    24901 ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa
    24961 ctttccgcag gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga
    25021 acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac aggccattac taccagcgtt
    25081 gccgccagca gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga
    25141 actaatccca gcagcgcgcc cacaattaac aacgcattaa gcatcaggct ggcagtcagc
    25201 gcatcttcca gaccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat
    25261 gggattacga ggcagacaaa gaagatgctg ttaaacgccg tgcgccgcca gtaacgcgaa
    25321 gagaacaagg ttttgatgtg tttatgggtc gccgttacca cttcatcgcc cagtaaaaca
    25381 tggggaccaa aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcacagt
    25441 agccaacgtg gcgattctgg cgttccccag cgtaataacg taatcaacaa cgcgggcaga
    25501 gctgccgatg ccagcaacca gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg
    25561 gcaatacttg ccaacacata gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata
    25621 ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt gacctactga gtaatcgcct
    25681 cccagaccaa tgccgatcaa aatgcgcagc ccaataagat gctctggcgt ggtggcaaaa
    25741 aattgcaaga acgaagcgag cgtaatcagc aaaaagctga aggtgaagat tttttgccga
    25801 ccaatatggt cggagatcca cccaagaacc aggctgccaa gaaacaaacc aaggagcgcc
    25861 gagccgccgc tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagttgaata
    25921 atggcgtaac caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc
    25981 gcagcaattc gacagtgaaa acggttaagc ggtaaatcat ccattcgcac cggtgaagtg
    26041 ttcatcttta ccccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa
    26101 aggcaacgcc ccgtcatcgc ggaacgctgc ctgatacatt atttttcaat cggatagata
    26161 gtacccgtat tcataatgcc gttaggatcg aactgctttt tcagcccttc cagcaacgcc
    26221 cacgcgctgc cgtgttccag cttgctccag tgaacgcgat gtttaccaat gccatggtgg
    26281 tgcaccatcg aaccgccgag gcgaatggtt tcttcgcaga tgattttgtt gagcggattg
    26341 tggtacttgt cgatttcctc ttccggctta cagtcaacaa cgttgtagtc atagacgaag
    26401 tacatgttgg tgccgttctg atagctatga gaggagtgac cgcccagcat ggtgatgtcg
    26461 tcggcgtgcg ggaactcagt acgaatacgg ttaataacgc tttcgtagat ttcgtggatg
    26521 cagctccagc agccggacac ttcggtggta aagcccatgt tgccggtttt gaggatctgc
    26581 acacgttcgg cagccacttt atccggtccc cagttcaggt tgttaaacca ggtttcaatc
    26641 agtttgctgt ccacgcgctg gcactgcggg tagcgggcaa cgatttccgc aatcccttcg
    26701 cccgtcgcct tcgcaatgcg agggttacct tcagccataa agatcagcac ccattttccg
    26761 tcggcaaaat gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac
    26821 ggacgatacc cttccaccat gatttcacgc aggatgttga agccggtttt catgtcttcc
    26881 aggatatagc cgtagaagag gttgttttcc ggggtgtatt taaagatttt cactgttact
    26941 tcagtgatat agcacaatgc accttcgttg ccgatgatga tgtgacgaat gtccgggcca
    27001 gccgcgcggc gtggcacgtt tttaatgcgt gtgacggtgc catctgccaa cactgcctcc
    27061 agaccaacga ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg
    27121 gttgctacca ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg
    27181 taaccttttt cacgcaacgc gttttccagc acttccagcg gaacaccaca ttgcgccgtc
    27241 gcctgcatat tctcaatatc aatattaatg atttgattca tggcggagcc gtcgagcacc
    27301 accgagtttt ctacaacagt ttccagccca ccttcggtgg cggaagcacc ggtacgcggc
    27361 acaccgttaa ttttgtgcgc attcataaaa ttcagcacac gggatactta ctctgtggaa
    27421 ccgagtttta cgaccgctgc cggaatcggc aaagtataaa tgccatgaat atccggaaat
    27481 ttacgaaaac ggtcaatact gtttttcttt aatactgttt catcggtaat tacgcgatct
    27541 gcaccaacaa tttcctttag ctggtcgaca atcgctgcgc gagataaaga catagtaatt
    27601 ccttcctgat aataaaaatg gaataatcat taataagaga aaacagaaac caccgttatg
    27661 gattagcgca ctaaataacc gccatcgacc actaataaat gcccgttgac ataattcgat
    27721 gcctgacttg cgagaaacac ggctgcgccc attaaatcct gagtatcgcc ccaacggttt
    27781 gccggaatat gatcaagaac gcgctgattg gtttctggat tactgcgtgt cgccagcata
    27841 atatgctgtt gcataatagc caggggcgat accatttacc tgaatattat attgacctag
    27901 ttcatcacaa taagctttgg tgaacccggc aagagcatgt ttagtggcag aatatgcagg
    27961 tgaccattgt ccacctaagt aagagaacaa tgaacagata ttaataattt tgccgctttt
    28021 ctgcgggatc ataatttttg ccgcttcata gcttaactcg aatgcggcgg tcaggttcac
    28081 atcaatcatc ggatcccagt cggcacgacc gaagtccagc accttattca gcttacaaat
    28141 accggcattg ttaaccagaa tatcgacagt accgaagcgc tcacagcagg cagcgataat
    28201 cttctgcggt gcgccttctg cggtaatatc cacctgcatg aagtccactt caacaccctg
    28261 tttttcaatc atttcctttg tttcgccgtt atctttgacg aagctaggaa taaaaatatt
    28321 tgcgccagct ttggccaacg ccattgcaaa tgcctgacct aaaccgctat tcccgccggt
    28381 aacaattgct gttttacctt tcagggagaa aaaatccatt gagaacgcat tgagagattc
    28441 gattgacata gttagctcca atttccgtaa ggcaaaaaaa aagaaaggta acttcccccg
    28501 cgagagggaa gttacctttc tcattatctc tggtaactgg agataatttt tagcataacg
    28561 gcataactgc taatgtgatc gtaatcacag tgtgatattc gtattgtcaa atagttttaa
    28621 aaagcagaag cgtgattact ctcacacaac acctctgctt tccatgttat ttcttattgc
    28681 aattactaga gacctgagaa aagtaatcca cctgaaaggg cggcttactt ttctcttttt
    28741 tttgtctatt acaaatggga atacgatatg caacacaact catatcgccg ttggataacc
    28801 ctcgcgataa ttagttttag cggcggcgtt agttttgacc tggcttattt acgttatatt
    28861 tatcaaattc ccatggcgaa atttatggga ttcagcaata ccgagatagg tttaataatg
    28921 agtacctttg gtattgcggc tattattctt tatgccccca gcggcgttat tgccgataaa
    28981 ttttcacacc gcaaaatgat tacttccgcg atgatcatta ccggattact gggtctgtta
    29041 atggcaacgt atccaccgct gtgggtaatg ctctgtattc aggtcgcctt tgcgataacg
    29101 acgattttaa tgctgtggtc ggtgtcgatt aaagccgcct cgttgcttgg cgatcatagc
    29161 gagcaaggga aaattatggg ctggatggaa gggctgcgcg gcgtcggtgt aatgtcgctg
    29221 gcggtgttta ccatgtgggt cttttctcgc tttgcaccgg atgacagcgc cagcctgaaa
    29281 acagtcatta tcatctacag tgtggtttac atcttgttgg ggattctgtg ctggtttttt
    29341 gttagcgata acaacaacct gcgcagtgcc aataacgaag aaaaacagtc attccagctt
    29401 agcgacatcc ttgccgtttt gcgtatcagc accacctggt attgcagcat ggtgattttt
    29461 ggtgtcttca caatctacgc cattctgagt tactccacca actatctgac cgaaatgtac
    29521 ggcatgtcgc tggtggcagc gagctacatg gggattgtga tcaacaaaat cttccgcgcg
    29581 ctgtgcggcc cacttggagg cattatcacc acctacagta aagtgaaatc ccctacccgc
    29641 gtgattcaaa tcctttccat catcggtttg ctggcgttga cagccctgct cgtcacgaac
    29701 tctaacccgc aatcggtcgc gatggggatt ggcctgattt tactgctggg attcacctgt
    29761 tacgcctcac gcgggctgta ctgggcctgc cccggcgaag cgagaacacc gtcttacatt
    29821 atgggcacca cggtaggtat ttgttcggtg attggattcc tgccggatgt cttcgtttac
    29881 ccgattatcg gccactggca agacaccctg cccgctgcag aagcctaccg caatatgtgg
    29941 ctgatgggca tggcggcgct tgccatggtg atagtcttta cctttttgct gttccaaaaa
    30001 attcgtactg ctgatagcgc ccccgcaatg gctagcagca agtaagccag gaaaattaca
    30061 ggagaaaaat catgtcgaaa aaatacatca tagggattga tggcggaagt cagagcacaa
    30121 aagtggtgat atacgatctg gaaggtaatg tggtttgcga agggaaaggc ttattgcagc
    30181 cgatgcacac gccagatgcc gatactgcag aacatcctga cgacgattta tgggcatcat
    30241 tatgttttgc cggtcacgat ttgatgagtc agtttgccgg gaataaagaa gatattgtcg
    30301 gtattggtct gggatccatc cgttgctgcc gtgcgttatt gaaagccgat ggcacggccg
    30361 ttgattagct ggcaggatgc acgcgtaacg cgtccctacg aacacactaa tcctgacgtg
    30421 gcatatgtca cctctttttc gggttatctg acgcatcgct taaccggcga gtttaaagac
    30481 aatatcgcca actattttgg tcagtggccg gtggattata agagctgggc atggagcgaa
    30541 gatgctgcgg taatggagaa gtttaatatc ccccgtcata tactgtttga tgtgcaaatg
    30601 ccaggcactg ttcttgggca tatcacacca caagccgcgc tggcgacaca tttcccagct
    30661 gggctgccgg ttgtttgtac caccagtgat aaaccggtag aagctctggg ggccggatta
    30721 ctggatgatg aaacggcggc gatttcactc ggtacttata tcgcgctgat gatgaacggc
    30781 aaagccttgc cgaaagatcc ggtggcgtac tggccgatta tgtcttctat tccgcaaaca
    30841 ttgctgtatg aaggttacgg tattcgcaaa ggtatgtgga cggtgagctg gctgcgcgac
    30901 atgttaggcg agtcgttaat tcaggatgcc agggcgcagg atctttcacc ggaagattta
    30961 ctcaacaaga aagcctctag tgtgccacct ggctgtaatg ggctgatgac ggtgctggat
    31021 tggctgacca atccgtggga accgtacaaa cgcgggatta tgatcggctt tgattccagc
    31081 atggattacg catggatata tcgttcgata ctggaaagcg tggcgctgac gctgaagaac
    31141 aattacgaca atatgtgtaa tgaaatgaat cactttgcga agcatgtgat cattactggc
    31201 ggcggttcga acagcgatct gtttatgcag atttttgccg acgtgttcaa ccttccggca
    31261 cgacgtaacg ccattaacgg ttgtgcaagc ctgggggcag cgattaatac agcggtaggt
    31321 ctggggctat acccggatta cgcaacggct gtcgacaaaa tggttcgcgt gaaagatatc
    31381 tttatgcctg ttgagagcaa tgccaaacgc tacgacgcga tgaataaagg tattttcaaa
    31441 gacctaacca aacatactga tgtgatcctg aaaaaatcgt atgaagtgat gcatggggaa
    31501 ttggggaatg cggattcgat ccagagctgg tcgaatgcgt aagtgaggaa gaccgggcgg
    31561 gaaactgccc ggcctgaata tacctgaatg gttatccccg ctgacgcggg gaactcgaat
    31621 aactgaccaa cgtctggctc tctaaatccg gtttatcccc gctggcgcgg ggaacactct
    31681 aaacataacc tattattaat taatgatttt ttaagccagt cacaatctac caactttaca
    31741 ggatcacaca aacaacacat ccattatgtt aaagagcact taatccattg attaaaaagg
    31801 taaatattta aaataactct atacaaacta aaatctacca aacttcgccg caataatttt
    31861 cactccagcg aaaaattaac gccgcagaat ttgtagagaa tgataatgat tagtccggga
    31921 tgggcaagat ggatgagttt tcaggggcga caaataatac aaatgagtgg aattttttac
    31981 aagttccacc catttataac
//
LOCUS       DAJTNM010000034        31833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTNM010000034 DAJTNM010000000
VERSION     DAJTNM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.034"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..218
                     /locus_tag="OO172_001688"
     CDS             <1..218
                     /locus_tag="OO172_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5511908.1"
                     /translation="IDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVDK
                     FSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(306..1604)
                     /gene="exuT"
                     /locus_tag="OO172_001689"
     CDS             complement(306..1604)
                     /gene="exuT"
                     /locus_tag="OO172_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5511909.1"
                     /translation="MSKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            2087..3499
                     /gene="uxaC"
                     /locus_tag="OO172_001690"
     CDS             2087..3499
                     /gene="uxaC"
                     /locus_tag="OO172_001690"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5511910.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHFELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3514..5001
                     /gene="uxaA"
                     /locus_tag="OO172_001691"
     CDS             3514..5001
                     /gene="uxaA"
                     /locus_tag="OO172_001691"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5511911.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            5084..5635
                     /locus_tag="OO172_001692"
     CDS             5084..5635
                     /locus_tag="OO172_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5511912.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(5640..6884)
                     /gene="sstT"
                     /locus_tag="OO172_001693"
     CDS             complement(5640..6884)
                     /gene="sstT"
                     /locus_tag="OO172_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5511913.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(7480..8445)
                     /gene="alx"
                     /locus_tag="OO172_001694"
     CDS             complement(7480..8445)
                     /gene="alx"
                     /locus_tag="OO172_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5511914.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(8716..8916)
                     /locus_tag="OO172_001695"
     CDS             complement(8716..8916)
                     /locus_tag="OO172_001695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5511915.1"
                     /translation="MQDLTQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLL
                     TEIRRQTGVIFPADSVKLYLPK"
     gene            complement(8947..9714)
                     /locus_tag="OO172_001696"
     CDS             complement(8947..9714)
                     /locus_tag="OO172_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5511916.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSEC"
     gene            complement(9793..10485)
                     /locus_tag="OO172_001697"
     CDS             complement(9793..10485)
                     /locus_tag="OO172_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5511917.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(10562..11065)
                     /locus_tag="OO172_001698"
     CDS             complement(10562..11065)
                     /locus_tag="OO172_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5511918.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            11150..12286
                     /gene="rlmG"
                     /locus_tag="OO172_001699"
     CDS             11150..12286
                     /gene="rlmG"
                     /locus_tag="OO172_001699"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5511919.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            12571..12885
                     /gene="higB"
                     /locus_tag="OO172_001700"
     CDS             12571..12885
                     /gene="higB"
                     /locus_tag="OO172_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5511920.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            12882..13298
                     /gene="higA"
                     /locus_tag="OO172_001701"
     CDS             12882..13298
                     /gene="higA"
                     /locus_tag="OO172_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5511921.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13343..15361)
                     /gene="fadH"
                     /locus_tag="OO172_001702"
     CDS             complement(13343..15361)
                     /gene="fadH"
                     /locus_tag="OO172_001702"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5511922.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(15581..17932)
                     /gene="ygjK"
                     /locus_tag="OO172_001703"
                     /pseudo
     CDS             complement(15581..17932)
                     /gene="ygjK"
                     /locus_tag="OO172_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(17949..18368)
                     /locus_tag="OO172_001704"
     CDS             complement(17949..18368)
                     /locus_tag="OO172_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511923.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(18346..18699)
                     /locus_tag="OO172_001705"
     CDS             complement(18346..18699)
                     /locus_tag="OO172_001705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511924.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(18700..19020)
                     /locus_tag="OO172_001706"
     CDS             complement(18700..19020)
                     /locus_tag="OO172_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511925.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(19154..20587)
                     /locus_tag="OO172_001707"
     CDS             complement(19154..20587)
                     /locus_tag="OO172_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5511926.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(20650..21099)
                     /locus_tag="OO172_001708"
     CDS             complement(20650..21099)
                     /locus_tag="OO172_001708"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5511927.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATHALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(21096..24182)
                     /gene="ebgA"
                     /locus_tag="OO172_001709"
     CDS             complement(21096..24182)
                     /gene="ebgA"
                     /locus_tag="OO172_001709"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5511928.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(24365..25348)
                     /gene="ebgR"
                     /locus_tag="OO172_001710"
     CDS             complement(24365..25348)
                     /gene="ebgR"
                     /locus_tag="OO172_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5511929.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            25567..25898
                     /locus_tag="OO172_001711"
                     /pseudo
     CDS             25567..25898
                     /locus_tag="OO172_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(25940..27319)
                     /gene="ygjG"
                     /locus_tag="OO172_001712"
     CDS             complement(25940..27319)
                     /gene="ygjG"
                     /locus_tag="OO172_001712"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5511930.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            27736..29256
                     /gene="aer"
                     /locus_tag="OO172_001713"
     CDS             27736..29256
                     /gene="aer"
                     /locus_tag="OO172_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR5511931.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(29410..30033)
                     /gene="nfeR"
                     /locus_tag="OO172_001714"
                     /gene_synonym="yqjI"
     CDS             complement(29410..30033)
                     /gene="nfeR"
                     /locus_tag="OO172_001714"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5511932.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            30310..31056
                     /gene="nfeF"
                     /locus_tag="OO172_001715"
                     /pseudo
     CDS             30310..31056
                     /gene="nfeF"
                     /locus_tag="OO172_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(31110..31185)
                     /locus_tag="OO172_001716"
     tRNA            complement(31110..31185)
                     /locus_tag="OO172_001716"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31150..31152),aa:Ile,seq:cat)
     gene            31310..31816
                     /gene="mug"
                     /locus_tag="OO172_001717"
     CDS             31310..31816
                     /gene="mug"
                     /locus_tag="OO172_001717"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR5511933.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         7683 a         8313 c         8382 g         7455 t
ORIGIN      
        1 aaattgatgt gcgcaataat gaaaatatgg tctggaacag cattaacctg aaacgtgtgc
       61 gtccggtggt actgcccggt attcgctatg ccgtgatgtg tgtgccgaca cccttgactc
      121 tggcagtcga taaattcagc gtgatggata aacaggccgg gtactacatg ggcaaattgt
      181 cagtaatctt tacgccttcc ttgccaacca tcaattaacc ctgcaaaccg gacaaacgct
      241 tttttctata taaacgacga agccgcctga taaggcggct tcgtcgttat aacttacgtt
      301 aaacgttaat gttgcggtgc gggatcgtta tgtgtttcct gtgccacctc aatcgccggt
      361 ttgttctgca atacggtcca gataaccagc gcacccagca ggtcaaatac tgccagaact
      421 gcgaacagcg ggctgaagcc gatggtgtca gccagtgcac cgacaaccag cgcaaacagc
      481 gtacttgcca gccatgcgga catcccggtt aaaccgttcg ccgtcgccac ttcgttacga
      541 ccgaacacgt cagaagagag cgtaatcagc gcgccagaca gtgcctggtg ggcaaaacca
      601 ccgatacaca gcagcataat tgcgacatac gggttggtga acagaccgat catacccggg
      661 ccaatcatca gcacagcacc cagcgttacg accatcttac gggaaacgat caggttcaca
      721 ccaaaccaac gctggaacag cggcggcagg taaccaccga ggatacaacc gaggtcagca
      781 aacagcatcg gcatccaggc gaacatcgcg atctctttca ggttaaagcc gtaaacttta
      841 aacatgaaca gcgggatcca cgcgttaaaa gtaccccagg ccggttctgc taggaaacgc
      901 ggcagtgcga taccccagaa ctgacggtta cgcaggatct gaccaacgga cattttcttc
      961 gccgtgctca cctggtgctg ggcttcctga ccattaataa tatagtcgcg ttcttcatcg
     1021 gtcagatgct tctggtcgcg cggatgttta tagaaaatca gccatgccat cgcccagata
     1081 aagctcaatg caccggagat gataaatgcc atctgccagc tgtgcatcac gattgcccat
     1141 accaccagcg gcggcgcaat catcgcacca atcgaagaac ctacgttaaa gtagcctact
     1201 gcgatggaac gctctttcgc cgggaaccat tcggagctgg ctttcagacc cgccgggatc
     1261 atcgctgctt ccgcggcacc gaccgcacca cgagcaacag ccaggccacc ccagctacct
     1321 gccagcgcgg ttgcaccaca gaacacggcc cacagcacag caaacattgc ataaccgatt
     1381 ttcgtaccca gcacatccag tacataacct gctaccggtt gcatgaccgt ataagcagca
     1441 gaataggctg cgatgatata ggaatactgt tgggtggaga tgtttaactc ttccatcaga
     1501 gttggcgcag ctgccgccac agtgttacgc gtcaggtaac caagcacggt gccgagcgtc
     1561 accagtgcga tcatatacca acgtaaccct ttaattttac tcatgtaaaa cctcatcgtt
     1621 ttgttatatc cgcaggaagc gcggccatca tcccgccaga ggctgaagat gttagttacg
     1681 accgggtaac cgaaaaaaaa ccggcacgcc ccgaacgttg ccatgaaagt ttcgtgctaa
     1741 ttcggcttcc gtaccggtta acccgattca tctaacaaat gaatgaatcg gcaatttgca
     1801 ttccgtcgac ttatgatttg cgacggcaga aagataactt gtcatacaac cttaaaaggt
     1861 gagagccatc acaaatgtgc gaatatttgt agggacatta cctgacgaca gcgaggccag
     1921 tactggcgcg gcctgcagcg agatttacca ctttgagagt aattttttta actacgttta
     1981 ttgatctaac tcacgaaaat atcttcggac tctggaaatt ggtgtgataa ctttgtcagc
     2041 atcgcaccat aagcaagcta gctcactcgt tgagaggaag acgaaaatga ctccgtttat
     2101 gactgaagat ttcctgttag ataccgaatt tgcccgccgt ctgtatcacg actacgcaaa
     2161 agaccagccg attttcgatt accattgcca tttgccgccg cagcagattg cggaagacta
     2221 tcgttttaaa aacctgtatg acatctggtt gaagggcgat cactacaaat ggcgcgctat
     2281 gcgtaccaac ggtgtggctg agcgtctgtg tactggtgat gcgtctgacc gtgaaaaatt
     2341 tgacgcctgg gcggcgactg ttccgcacac tatcggcaac ccgttatacc actggacgca
     2401 cttcgaactg cgtcgtccgt tcggtatcac tggcaaattg ctttctccgt caactgccga
     2461 tgaaatctgg aacgaatgta acgaactgct ggcgcaggat aacttctccg cgcgcggcat
     2521 catgcagcag atgaacgtga aaatggtcgg caccaccgat gacccgatcg attctctgga
     2581 gcatcacgca gagatcgcca aagacggctc tttcaccatc aaagtgctgc cgagctggcg
     2641 tccggacaaa gccttcaata tcgaacaggc gacctttaac gactacatgg cgaagctggg
     2701 cgaagtttcc gataccgaca ttcgccgctt tgctgacctg caaactgcct tgaccaaacg
     2761 tctggatcac ttcgccgctc acggctgtaa agtgtctgac catgcgctgg acgtggtgat
     2821 gtttgctgaa gcgaacgaag cggaactcga cagcattctg gcgcgccgtc tggctggcga
     2881 aaccctgagc gagcacgaag tggcacagtt caaaactgcg gtactggtgt tccttggtgc
     2941 cgaatatgca cgtcgcggct gggtacagca gtaccacatt ggcgcgctgc gtaataacaa
     3001 cctgcgtcag ttcaaactgc tggggccgga tgtaggcttt gactccatca acgaccgtcc
     3061 gatggcggaa gagttgtcta agctgctgag caagcagaac gaagaaaacc tgctgccgaa
     3121 aaccattctc tactgcctga acccgcgcga taacgaagtg ctgggcacca tgatcggtaa
     3181 cttccagggc gaaggtatgc cgggcaaaat gcagttcggt tccggctggt ggtttaacga
     3241 tcagaaagac ggtatggaac gtcagatgac ccaactggcg cagctcggtc tgctgagccg
     3301 ttttgtcggt atgctgactg acagccgtag cttcctgtca tacacccgtc acgaatactt
     3361 ccgccgcatt ctgtgccaga tgatcggtcg ctgggtggaa gcgggcgaag caccggcgga
     3421 catcaacctg ctgggcgaga tggtgaaaaa tatttgcttt aacaatgcgc gtgactactt
     3481 cgccattgaa ctgaactaag gtctggggtt gatatgcaat acatcaagat ccatgcgctg
     3541 gataacgtcg cggtcgcttt agcagatttg gctgaaggca cagaagtcag tgtcgataac
     3601 cagactgtta cgctgcgcca ggatgttgct cgtggacata aatttgcgtt aacggatatc
     3661 gcaaaagggg ccaacgtcat caaatatggc ctgccgattg gttatgcatt ggcggatatt
     3721 gcggcggggg aacacgtcca cgcccacaat acgcgcacga atctgagcga tctggatcag
     3781 tatcgctatc aacctgattt tcaggatctg cctgcgcaag cggcagatcc tgaagtgcag
     3841 atctatcgtc gcgctaacgg cgatgtcggg gtgcgtaacg agctgtggat cctgccaacc
     3901 gtgggctgtg tcaacggcat cgcgcggcaa atccagaacc gtttcctgaa agagaccaac
     3961 aacgccgaag gtaccgacgg cgtgttcctc ttcagccaca cctacggctg ctcacaactg
     4021 ggcgacgatc acatcaacac ccgcaccatg ctgcaaaaca tggtgcgcca cccgaacgcg
     4081 ggcgcagtgc tggtgattgg tctgggctgt gaaaacaacc aggttgccgc attccgtgaa
     4141 acgttgggcg atatcgatcc tgaacgcgtt catttcatga tctgccaaca gcaggatgat
     4201 gagatcgaag ccgggatcga gcatttgcat cagctgtata acgtgatgcg caacgataaa
     4261 cgcgagccag gaaaactcag cgaactgaag tttggtctgg agtgcggtgg ttctgacggt
     4321 ctttctggta ttactgctaa cccgatgctg gggcgtttct ctgactacgt gattgctaac
     4381 ggcggtacta ccgtactgac cgaagtgccg gagatgtttg gcgcagagca gttgctgatg
     4441 gaccattgcc gcgacgaagc aacgtttgaa aaactggtca ccatggtcaa cgacttcaaa
     4501 cagtacttta ttgcccatga tcagccgatc tacgaaaacc catcaccggg gaacaaagcg
     4561 ggcggtatca ccacgctgga agacaaatca cttggctgta cccagaaagc gggttccagt
     4621 gtcgtggttg acgtgctgcg ttacggcgag cgtctgaaaa cgccagggct gaacttgtta
     4681 agtgcgccgg gtaacgatgc cgtagcgacc agcgccctgg cgggtgcggg ctgccatatg
     4741 gtgctgttca gtactggtcg cggcacgcca tacggtggat ttgtgccgac ggtgaaaatc
     4801 gccaccaaca gtgaactggc ggcgaagaaa aaacactgga tcgactttga cgcgggtcag
     4861 ctgatccacg gtaaagcgat gccgcagttg ctggaagaat ttatcgacac catcgttgag
     4921 tttgccaacg gtaagcaaac ctgcaacgag cgtaacgact tccgcgaact ggcgatcttc
     4981 aaaagcggcg taacgctata aagtcgaaat gagttgttaa taaaaaacgg cgtttcataa
     5041 tgtgaagcgc cgtttgcttt tccatacctg ctaaggattt atcatgaccg cgtattggct
     5101 ggcccagggc gtgggtgtca tcgcctttct gattggtatc acaacatttt tcaatcgtga
     5161 cgaacgtcgc ttcaaaaagc agctttcggt ctatagcgcc gttattggcg tacatttttt
     5221 tcttctgggc acctatcccg ctggtgccag tgccatcctt aatgccattc gtacattgat
     5281 taccttacgc acgcgcagct tatgggtaat ggcgattttt attgtgctga ctggcggaat
     5341 tggcctcgcg aagttccatc atcctgtcga actattgccg gttatcggta cgattgtcag
     5401 tacctgggcg ctgttccgct gtaaagggct caccatgcgc tgcgtgatgt ggttttcaac
     5461 gtgttgctgg gtgattcaca acttctgggc ggggtcgata ggcggcacga tgattgaggg
     5521 tagttttctg cttatgaatg gcctgaatat cattcgtttc tggcggatgc agaaaagggg
     5581 aattgatccg tttaaagtag agaaaacccc tcccgccata gacgaaaggg gttaaacaat
     5641 taattacgca gggcgctatt tgccagacga tcgtcttccg cctggcaagc tgccgcagtg
     5701 aacagcacgt cagttgaaga gttcagcgcg gtttcgcaag agtcctgcaa tacgccgata
     5761 ataaagccga cggcaaccac ctgcatggcg atatcgttcg aaataccgaa catattacag
     5821 gccagtggga tcagcagcaa agaacccccc gccacgccgg atgcgccaca ggcacacaga
     5881 gaagccacta cgctcaacag cagcgctgtg ggcagatcga ccggaatacc cagcgtatta
     5941 accgcagcca gcgtcaacac ggtaatggtg attgctgcgc ccgccatatt gatggtggct
     6001 cccagcggaa tagaaactga ataggtatcg cgatccagat tcagcttttc acacagcgcc
     6061 atattcaccg gaatgttcgc tgcagagctg cgggtgaaga aggcatacac accgctttcg
     6121 cgcaggcaca gcagcaccag cgggaacggg ttacgacgaa ttttccacca caccagcaat
     6181 gggttaacca ccagcgccac cagtaacata cagccaacca gcacgaccag cagttgcgcg
     6241 tagccccaca gtgtggagaa accggtggtt gccagggtag aagaaaccag cccaaaaata
     6301 ccgatcggtg cgaagcgaat gaccagtttc accataaagg taacggcatt cgacatatca
     6361 ttgaccaggt ttttggtggt ctcgttaccg tgacgcagtg cgaagccgag gccgatcgcc
     6421 cacaccagaa tcccgatgta gttacctttc agcagggcgt cgatggggtt ggaaaccatg
     6481 ctcattacca gtccgcgcat cacttcgaca atgcctgacg gcggcgaaat atcacccgcg
     6541 ctactggaca agtgcagggt agaagggaag gcaaagctga agactactgc ggccagagct
     6601 gcagagaagg tgcccagcag atagaggaac aaaataggac ggatattggt tttctgcccg
     6661 tgctggtggt tagcaataga tgccattacc agcatcaaca ccaggatggg ggcaacagct
     6721 ttcagtgcgc cgacgaacaa agtacctaac agaccaacag cttccgccgc gggttttgag
     6781 atccatgcca gaagaatccc cagaacaagg ccgaccagga tttgttttac caggctgcca
     6841 tgagccagac gccggaatag ccccggtgaa cgttgcgtag tcatttttcg atcctttcat
     6901 tgtgttgtcg cacatccctg gtgcgttctg tcgacgcatc tttcaggatg taacaaagtg
     6961 tttgcaaggt cgagtataag gaaagttaca gcttcgggaa gcgaaaaatg ctggatttta
     7021 aagagaggtc atgttttgca acttaatggt tgcatttatt taacataaac agagggaagg
     7081 actggctatt aaggctgatt agataacata tgggcaaaac tatttggaat atgagaacgg
     7141 ttgcgctcct ttaacgtgtt atgccatata aagtcactgg cgcagggagc gcgcaggggg
     7201 cggccaatcg ccgccgcccc ctgccccccc aggctctggc gaacaaaatc gccgctgcgc
     7261 ggttccctca gcctatccct tgcggctacc gggtcgggcg caaggtaacg tccctgtaag
     7321 atgcgccctc gccccggcct ccagggaacg cttcggcgat tttgacgcca ccaacacccg
     7381 tgcggtcttt ttgtaaagag ctataagccg attacataat taatagctca cctttgtctg
     7441 gaatgagtta tctctaggaa tggcttaata tacagcgaat cattctcccc gctgcttatc
     7501 atgccgataa ttcacccagg cgttgataat aaacgtcatc accagaatgc caaacaccac
     7561 gcccagcgag acggcgattg gaatatggta gaagtcgaca atcagcatct tgataccgat
     7621 aaacaccaga atcaccgcca ggccatattt gagcatcgag aaacgctctg ctacgcccgc
     7681 cagcaggaaa tacatcgcac gcaggccgag gattgcaaac aggtttgagg tcagcacaat
     7741 gaacgggtca gtggtcacgg cgaagatagc cggaatgcta tccacggcaa aaatcacgtc
     7801 gctcaattcc accagaatca gtaccagcat cagcggtgtg gcgtacagca atccattctt
     7861 acgcacaaag aaatgctcgt tgtcgatggt gtcggtcatg cgcaaatgac cgcgtagcca
     7921 gcgcaccagc ggcttgtcgc caatgcctga ttcatcttca tgggcgagcg ccatcttcac
     7981 gccggtaaac agcaggaagg caccgaagat atacaggatc cagtcgaact gcgaaatcag
     8041 ccagctgcct gtgaagatca tgatggtacg cagaacaatc gccccgagca cgccatacac
     8101 cagcacgcga cgttgtaatg ccgccggaac ggagaaatag ctgaacaaca tcagccagac
     8161 aaagacgtta tcgaccgcca gcgatttctc aatcagataa cccgtgagaa aggccagggc
     8221 ctgtggatcg gcaacggcgc gaccttcggt ttgcaccaga taccaccaga aagcggcgtt
     8281 aaacagtaac gacagcgtca cccagaccag cgaccaggcc gcagcctgtt tcatggtcat
     8341 ggcatgtgcc ccacgacgcc cctgcaacaa caggtcgata gccagcataa tggcgacaac
     8401 aacagcaaat ccgccccata gcaacggcgt gccgacagta ttcatagaag ttccttacac
     8461 ataaaaaaac ggccaacgtc ggaagacgtc agccgctgct ttttatgcat agacctcgcc
     8521 ttccggcaag gtctcactta caacaaaaaa ggaatacgtc tgcgtattcc tttcgggttg
     8581 cccggtgacc ggatgtggtt tttcacacat cgtaatgacg atcgaccggc aatgaagtta
     8641 ctcccctttg caggtaacaa agtatgacag accattcagt cagtcaatgg tctgttacat
     8701 accttttaca ctgttttact ttggcaagta tagttttacg ctatctgccg gaaaaatcac
     8761 cccagtctgg cggcggatct cggtcagcag tttggctgtg atgcgactga ccgccagccc
     8821 tggatgatcc accagatgct catccaccag ggtagcgaac agctctgctt cgtagagcat
     8881 ggtattaata tgctgcggct gggtgagatc ctgcatctgg ctgccacgcg gcacaaagca
     8941 gactttctag cattcagaca gtttttcaat caccagcgat cccgcttcgc cctgaatctc
     9001 gcttcccagg acagaatcac tgacctttga gtgctgcaag gtgacgctga aatcaccgta
     9061 atccatcacc accacgccgt gggcgtcaac gccgcttgcc agcaaactcg ccgttgcctg
     9121 cacgcttttc ggctcaccaa ataacgccac cgccgacgcc aggcagtaaa agccgatatc
     9181 cataattgaa ccgttagaga atgccggatt aaaggtgttg ggattctcac cgtccaggta
     9241 acgttgatac cgcgaggaat actggcaata gttgaaaaag actttacgca gtttgcctgc
     9301 tttcggcagc gcctggcgca acaaatgaaa gttcggcagg caggcggttt taaatgcctc
     9361 aaacagcacc acctgatttt cgcgcgcaca ggcaatggcg gcatccactt ccgccagatt
     9421 cgacgccagc ggtttttcgc aaatcacatg aattttatgg ctaaggaaaa gttgtgtctg
     9481 ggaaaagtgc agggaattcg ggctggcaat atacaccgcg tcaatggcat cgctttccgc
     9541 catcgcttcc agcgacgtaa acagatgctc gacagaaaaa tcattggcga agtgctgagc
     9601 ctgctcaagg ctgcgggaat atacggcggt taacttgtat ttaccgctct catgggcggc
     9661 ctcgacgaac tggcgagtga tccagttcgt accaatcaca gcgaaacgta tcataacgcg
     9721 tggatactcc ataaagggat tctgtcgtca atgtagcacg cctttttcat cttcgacgtg
     9781 cgcagatacg cattattctt tcgccggaca gccatgctca ctaaacggcc caatcatgac
     9841 cgatggcccg agatatttcg gctggcgatg gaggattttt gcgtcaatca ccgcgctgac
     9901 acgagcgagt ttctcccgca gttgagtata aaaaccatgc ttcatattga ggtctggcgc
     9961 gttaaacccg atagcgtcga tgccgtactg ttttgccagc cagatggcgc gttggttatg
    10021 aaattcttgc gagataatgg tgatatggtt ttccccaaac acctttttgg cacgcactac
    10081 cgaatccagc gttgagaatc cggcatagtc gcaaaagatc actttcgccg gtacgccttt
    10141 agcgatcaaa gcctgctgca tacctgatgc ttcatcgtaa tttttacgtc cgttatcgcc
    10201 actcaccagt agccatttca cttttccggc gtggtacaac gctgctgcgg tatcaatacg
    10261 tcgggtaaag tagcgattac ccggcctcgc ccccagcaat aaacccacgt tacgcgccgg
    10321 gacggcgttg acatcgcccc aggtcagctg cttgctggcg ttgaccataa cgcgatcggc
    10381 gataaaaatg gtcgctcctg ccacaagtaa cagtaagcag gcaatcttta gagtccggcg
    10441 agaaaaacaa atacggagaa gaaggcgggc aaatgcgcgc agcatggtcg ttatccttaa
    10501 cgaaaggggg gaggtcgatt ttaggcgcta aagaaagaaa agataacgcc tgacatcatt
    10561 ttcagatttt gtcctgccca agcgacaact gcgttagcca cagccgggtg tcgaactcaa
    10621 gctggtggta ctgcggctcc atatggcagc acaattgata gaacgctttg ttatgctctt
    10681 tctccttcag gtgcgccagc tcgtgcacca cgatcatgcg caaaaacggt tccggcgcgt
    10741 tgcgaaacac ggtagcgacg cggatctctg cttttgcttt cagcttgccg ccctgcatac
    10801 gagaaacagc ggtatgtaac ccgagcgcgt tttttagcac atggatctta ttgtcataca
    10861 tcactttatt gatcggcggg gcattacgca gaaactgatt tttcagatcc tgagtatatt
    10921 gccagagagc tttatcggtg gcgtagtcgt gtgttcccgg atagcgtttt gccagcacat
    10981 cgcccagacg ctgttcgtta atcagtgttc gcacctgcga aagcagctgc tcgggataac
    11041 cctgaagata agtaagattg ctcataaccg cccacgaaaa agagaaaaag ggtatactca
    11101 cgcacccttt tcaggggcaa cgtcgaaatt ttatcattca ggagggccga tgagccactt
    11161 agacaacggt ttccgttcac tgacactaca acgttttccg gcgacggatg acgttaaccc
    11221 gctacaggcg tgggaagcgg cagatgaata tttgctgcaa cagttggacg acacagaaat
    11281 ccccggcccg gtgttgatcc tgaatgatgc ctttggtgcg ttaagctgcg cgctggcgga
    11341 acataagccg tacagcattg gcgactcata catcagtgaa ctggcgacgc gcgagaattt
    11401 acgcctaaac gggattgatg agtcgagcgt gaagtttctc gacagcaccg ccgactaccc
    11461 gcaacagccg ggtgtggtgc tgatcaaagt gccgaaaaca ctggcattgc tggaacagca
    11521 actgcgtgcg ctgcgcaaag tggtcacgtc ggatacacgt attattgccg gtgccaaagc
    11581 ccgtgacatt cacacttcca cgctggaact gttcgaaaaa gtgctcggcc cgaccaccac
    11641 cacgctggca tggaaaaaag cgcgcctgat taattgcacg ttcaatgaac cgccgctggc
    11701 cgatgcgccg cagaccgtta gctggaagct ggaaggtact gactggacta tccataacca
    11761 tgcgaatgtc ttctcccgca ccgggcttga tatcggcgcg cgcttcttta tgcaacatct
    11821 gccagagaat ctcgaagggg agattgtcga tctcggttgc ggtaatggcg ttattggtct
    11881 gacgctgctt gataaaaacc cgcaggcgaa agtggtgttt gtcgatgaat cgccgatggc
    11941 ggttgcttcc agccgtttga acgttgaaac caacatgcca gaggcgttgg atcgcagcga
    12001 gtttatgatc aacaacgcgc tctccggcgt ggagcctttc cgctttaatg ctgtgctctg
    12061 caaccctccg tttcaccaac agcatgcgct gaccgataac gtcgcctggg agatgttcca
    12121 ccacgcccgc cgctgcctga aaatcaacgg cgagctgtat atcgttgcca accgccatct
    12181 ggattacttc cataaactga agaaaatttt cggcaactgc accactattg cgacgaataa
    12241 taaatttgtg gtgctgaaag cggtgaagct gggacgtcgt cggtaagatt gggagttacc
    12301 tgccggatgc ggcgtaaacg ccttatccgg cctacgttca gcacgttacc cccaggccgg
    12361 ataagacgcg gcaagcgtcg catccggcaa tgtctgctca gttctggaat cgcgcttcca
    12421 taaaacaaaa aatccctgtc accatcctct acattctctt gtttagcgtt tttctacgtt
    12481 tattcttccg tcacacagat aaattcatcc gttgccaatc tgtcaacgga tgttatcata
    12541 ttgccaattt gtcaacggac gtgatgaaga atgcacctga taactcaaaa agcattgaaa
    12601 gatgctgcgg aaaaataccc gcaacataaa acggagttgg tggctctggg gaacacgatt
    12661 gctaagggat atttcaaaaa acctgagtca ttaaaagcag tattcccatc tctggataac
    12721 ttcaaatatc tggataagca ttatgttttc aatgttgggg gcaatgaatt acgtgttgta
    12781 gcaatggtct tttttgaatc gcaaaagtgc tacatacgtg aagttatgac gcataaagaa
    12841 tacgatttct ttaccgctgt tcatcgtact aaggggaaaa aatgattgcg attgccgaca
    12901 tcttgcaagc aggagaaaag ctaactgctg tggcaccttt tctggcgggt attcagaacg
    12961 aggaacaata cacccaggcg ctggaactgg tagatcatct gctgctcaac gatcctgaaa
    13021 accccttgct ggatctggtg tgtgccaaaa taaccgcgtg ggaagaatca gcgcccgaat
    13081 ttgcggaatt taatgccatg gctcaagcca tgcctggcgg tatcgccgtg attcgtaccc
    13141 ttatggatca atatggttta accctttccg atctgccgga aattggcagt aaatctatgg
    13201 tgtcacgcgt tttgagcggg aagaggaaat taacgctgga acacgctaaa aaattggcaa
    13261 cgcgattcgg catttctccc gccttgttta ttgattaacg cgacgtgccg gatgcgttgc
    13321 atccggcgct tcaaataacg atttaaatct ccagcgccag ccgtgttccc tgggcaattg
    13381 cccgtcgtgc gtccagctcc atagccacat cgcagccgcc aattaaatgc acagttttcc
    13441 cgctatcaat cagcggttgc gccagcgcgc ggtttggctc ttgccctgcg cagatcacca
    13501 cattgtccac tgctaatacc tgcgtttcgc cgttgatcac cacatgcagc ccgtcatcgt
    13561 caatcttctg ataacttacg cctgggatca ttttcacacc ccgcgagagc agggtggtgc
    13621 gatggatcca gccggtggtt ttgcctaacc cctgtcccgg tttgctggct ttgcgctgga
    13681 gcatcacaat ttgccgtggg ctacgcggga tctgcattcc ctgcgggctt aagccaccag
    13741 cctgttgtag gctgctgtcg atcccccact cattacagaa cctggcgata ttctggctgg
    13801 tggattcgcc cggctgactt aaatacatcg ccgtatcaaa accaatcccg ccacaaccga
    13861 tgatggcaac cttgttgcct accggcgctt tgtcgcgcag tacatcgaga taactcaata
    13921 ccttcggatg atcgatcccg tcgataggcg gaacacgcgg cacgatccca ctggcgagga
    13981 tcgtttcatc gaacgcctgt aactgatccg ccgtcacggt gtgattgagt ttcagcgtca
    14041 cgcccgtcac ttcgatcatc cggcggtagt agcgcagcgt ttcgtaaaac tcctctttgc
    14101 cggggatctg tttggcgata ttaaactgcc cgccgatctc gctgtgcgca tcaaacaatg
    14161 ttacctgatg cccacgcgcc gccgcgttaa tggcaaacgc cagcccagca ggtccagcgc
    14221 cgaccaccgc cagatttttt ttctgcacgg cgggaaggat tggcattttg gtttcgtggc
    14281 aggcgcgagg attcaccagg cacgaggtga ctttgccaac gaagatctga tcgaggcagg
    14341 cctggttgca gccaatacaa gtgttgatct catcggctcg ccctgattgc gcttttgaca
    14401 gcagttccgc atccgcgaga aacggtcgcg ccatcgatac tatatcggca tcgccgcgcg
    14461 agagaatatc gtcggcaacc tgcggatcgt taatccggtt ggtggttacc agcggcagcg
    14521 agacgtggcc tttcagtttg cgcgtgaccc agctaaatgc gccgcgcggc acgggcgtgg
    14581 cgatggtcgg aatccgcgct tcgtgccagc ctattccggt gttgataatg gtcgcgcccg
    14641 ccgcttcaat ggcctgcgcc agttctaccg tttcggcaaa agtgccgccg ccttcgacca
    14701 ggtcgagcat cgacaaccgg taaataataa tgaagtcgtt gccgacgcgt tcgcgcaccg
    14761 tacgcactac ttctacggca aatcgcatcc ggttgcggta atcgccgccc cactggtcac
    14821 tacgctgatt ggtgcgcagc gtcagaaatt cgttgatcaa atacccttca gagcccatca
    14881 cttcgacgcc atcgtatccg gcctcccgcg ccagttgcgc gcagcgggcg aaatcgtcga
    14941 tcagttgcag gatctcttca tgggtgagtt cgtgaggaac gaaacggttg atgggggcct
    15001 gcaatgcgga cggtgcgacc agatgcggtt ggtagctgta gcgcccggta tgcaaaattt
    15061 gcagggcgat tttgccgcct tcctgatgta cggcttcggt aatggtgcga tggtgtggga
    15121 tctggctggc gtcgttgagc attgcgccac cttccatgcc aacgcctatt aaatctggtg
    15181 caatgccgcc gctgacaatc agcgccacgc cgtgacgggc gcgttcggca taaaacgcag
    15241 ccagccgttc agcaccgtcc gggtattcct ccagcccggt gtgcattgaa cccatcaaca
    15301 cgcggttttt taacgtggta aaacctaaat ccagcggggc gaacagcgac gggtagctca
    15361 taaaattgtc cagtatgtaa aataattgtt acggatgagt tctaatttag ccgtcgatgg
    15421 agaaaaggga aaaggggagt gcggggtttg tgatgggatt caaaaagaat acgcccggtg
    15481 gtaacgccgg atgcgacgct tgccgtgttt tatccggctt gggggattgc acatgtaggg
    15541 cggataaggc gttcacgccg catccgccag ccgttgcaaa ttactgctta cggaaaaaat
    15601 cgttatacaa catatacaaa tgagcggcac tccaggagaa atttggtgcg ccttgctgcg
    15661 cgcctgtcag cgggttgtaa ttctcctgaa ttgggccatc ggcggttaat cctttggcgt
    15721 gcctgaagaa cgtatccgcc agcttcaggg catcatcacg ataaccgtaa cgctccatcc
    15781 ctttcagacc aaaccagaac tgatccaccc atacgcgccc gcgccagtag atatcagcgc
    15841 caaaagccgg attggttaac gccgccgttc ccagcggaac aaaggtgttg aactctttag
    15901 gatcgagcat caccttcacc accgcgtcgg cattggcctg cgttgccgca ccgttaaaca
    15961 gcggcgacca gccttccggc cctttaccgc gctcaacaat cggtttgccc gcgcagccgt
    16021 tcgccagtgg tttatcttca atacgtacgt catagtagaa ctgggtagtc gggtcgaaca
    16081 tacaggtgtt gatgtagtcc gcgagctgct gtgccaactg gcgatagcgt ttggcctctt
    16141 ctggcttacc gaggatggtt gccatctccg ccagataatg gttatcgctg tacatatagc
    16201 tggcctgatc caccgactcc tgcaatagcg agtagcccag caacgttccg tcctgactgc
    16261 ggttttcggc gaatttcacc gtctagtcgc tacgtttgcc gccattagcg acatatttat
    16321 ccaactgttc tttgtcgata aaaccaaaga cggcggcatc atcacgcccc gattcccacg
    16381 acgcagcgac ctgtgccgga atttccagac tgtcgtactg gcctttctcc accacgcggg
    16441 cgtagttgtt cagcccagac tgcgtctctt ctttatcgcc ttttttcacc gtaaacagca
    16501 tctcgccgct ctcggtgttg tgggctttgt cgcgggtcgc gccatattcc ggcaccccgt
    16561 tgccgttatg atcgcggtta cgtaaccacc agtcgtgata ggccaccagt ttcgggtaca
    16621 tctctgccaa ccaggcttta tcctgggtga cgttgtatac ttccatcacc gaccaggcgg
    16681 caaggctggg tttggtatta cgttcgttcc agttgccgcc atcgccacca cgctcggggc
    16741 taaggttcca cgctatcagg tcggggacaa agcccacatc ctgcggacgc acgctgtcgc
    16801 caggctggat ctgccaggag aagaccgcgc ggatattctc tttggcgata tccggattga
    16861 aatgcgccat cgcaaacgcc tgcttccagg tatcccacgg ccaggtctga ttgccggaga
    16921 accagcgtcc ggtcaccgac ggcgtgacgg tgttataatt caccgcaccg cccggtgagc
    16981 gccagttacc gttgagcgtt tcgatcgctt tcaccgcgac gcgcgtctgt tccggcgtcg
    17041 catccggatt ggttaaccct ttcttcaggt attcttccca gcgttgctgc gaggcggtga
    17101 gataaaacgc cggacgcgcc agaatatcgc ggatctgcat ttgctctttg ctaacttcct
    17161 gagcggtcag cagatgggaa taggtggtgt agagcgtggt cgaaccgttg atatgcgcct
    17221 tactggtaaa gcgattaccg ttgatttcag tctgcaccgg cagggattta tgcacctgat
    17281 attccgattc gccggaggtc agcagatccc aggtggcgcg cactttgcca aaggtgactt
    17341 tcaggccatc acgggtggcg ctgattttgc gctgatagtc agggtattcg ccagcaatgg
    17401 ttttatcgga aagcggtttc ccttctttcg cttccagttt ttccagcagt tcgccatccc
    17461 acaccagatc cagcggttta ttgctgatga ttttagtttc cagtagtgac gtgcgcggcg
    17521 tggcgaagcg cagagtcatt tcgacctgca catcttttgc tgtcagtttt tgcaccagcg
    17581 cgccgggaat actgtatgcc tccagcgtga agttgacttt cttgccgtct tgccagacag
    17641 tcaggcggtc gaaattgctg gccataaagt tgatgtattc ttccgtcagc agcgcaacgc
    17701 ccggaaagcc gcccatggtg ttcgggccgt ctggcaacag atgaccatgc caggctccga
    17761 gatcgaaaaa cggattaaaa cgctggtgat cgtcgtaatc gtaatccttc atgtactgcg
    17821 gcgcaccagt acggttaatc acgtttttat aattgtcggc gttagcggca tgagcggaaa
    17881 aacttatcag cagagcgcag gttactggcg ttaaaatagt tttgattttc atgaaattac
    17941 agtccttatt accagtagaa atattgcatc aggaagattt ggtcgctggg atcgttagag
    18001 gcatttttca tgtagaagcg gctatcaaaa gccgtggcga aacgtccgcc gccatattcg
    18061 taccagatac caaactgcat gaaagaggtg gtttcatcgt cagcgtggtc agggcgatgt
    18121 ttggcgtagg tgtaagcggt ggagagataa acgcccttta acgcttcata catggcgctc
    18181 accatcatcc cgctttcact tcccggtacg tccagcccgt cggcacgtcc ggtatggtgc
    18241 caggcgcgag cggcaaagtt aggggtaaac aggcccgtga gatagagttg tcctgtgccg
    18301 tcggtaattt ccagaccatt catccaggtg atgccttcgg caaggtcata ctggatgtaa
    18361 ccgttcatcc attgccggat aggtgtattt gtcgtcgtag cgatcgtact tgccaaaatg
    18421 tagccaggct ttgctttcgt catgatgtcc ggcaggtgtg gcggtgacgc tataacgaaa
    18481 gttgccgctt aagttctgta ccttcagcgc ggtcataatg tcgcgggtat tgggaatgac
    18541 gtagcccaaa tcgggtgtga agtcgcccca ccattgcagg tcatccagcg aggaatcctg
    18601 acgcagactg agcatgattt cggtgccatc attggtacga taaccgccat agaaacggtt
    18661 aatcccacct tcgaaaccac cccagctatg gtcaggcacc taggcattac cgtcgtggtc
    18721 ggcctgcacg gtccagcctt cgccgtaaat caggccgaac caggagccgt acttcatctc
    18781 caggccacct tcaatatagg tgccgtcgct gtatttatgt ttattctcac cctctaaatc
    18841 catatatcca ccaagcccta attcgccgta aaaacgaaat gaaggagaag atgtcgaggt
    18901 aattgcgggt gtttttgtgt cgtcatgttc tgccggacgt gcttcttctc ctgccgcaga
    18961 aaaggcgtag caaaacggca gagcaagtaa tgctctgcat ggtgcagtga ttaatttcat
    19021 tgtgttaatc catgagctaa agaaaaagta cagcctttta tcgttgtaat tataaaaata
    19081 taaaggcaga aactattttc cctctcctca tccaggaaga gggaaaatgc ctgctcattt
    19141 tttattagtc tgcttaatca acattgctgg ctggcgtcgc ttcaatatgg cgctcttcag
    19201 ccgttaatcc ctttatatat ttactgtatt tccaccacgc aaagccgagg aagataacaa
    19261 taccgccgac gttataaaaa atgatggtca gaatattcgc gccagtcggg aatgtcgaag
    19321 cgacaaaccc tacggcaaat atcgcaatca gcattgaaac aacgataata ccggtgcggc
    19381 gggagcccat acggaaatcg cgtggcaggt gatctaattt ggcgcgtaaa ttcaggtaag
    19441 ccagcatgat gaataacggc ggaagcatgg acgctgcggc ggtcatatta ataatggtat
    19501 tcatcagatc ctgcacggta ttggaaccga gcatcgggat aatcatcagc gggatgacga
    19561 tcagaaactg gatccatgct gcgcgcgccg gaacgccgtt ttcgttaagt tcgacggttt
    19621 tcttaccaaa gatgccttcc gggatttcgg agaagaaaat tttcaccggc gttgcggtcc
    19681 acatcagcag ggaaccgaac attgcggtaa aggacaccag accgacaaag cgattcatca
    19741 acgcttccgg cagaccaaaa tacgccgcca tgccgtggaa tacctgcacc gatccgccgg
    19801 taaatttcaa ctctttgctg ctgacgaaga cgttaatcag caccgaggag acggaataca
    19861 gtacgccgat aaaaatcccg gcgaggatga tcactttaac gaacgatttc gaaccgcctt
    19921 tgacgtcgtt aacgtacacc gcgacggact ccgcgccacc tgcggccata aagatccagg
    19981 tggtaacgcc gaggaacgcc cagttgaagt tcgggatcat cgcgtcaacg gtgatcggat
    20041 cggcaggctg tacgtcgcca accagtgccg tacctgccag taaaatgtag gagagcgtta
    20101 acagcagcat cagcgttgaa gtgacggagg taattggccc cagcattttc gccccgttgg
    20161 tggaaaccca ggtggagaag gcgaacagca ccatactgat aacggtggtg gcaaccggcg
    20221 tcataatata ttcgtagccg aggaaggcat acgaagcata agcaataacg cgcggcaaca
    20281 gcgaggtgaa aaagaacaga tttacgaacc agtaagtgta ggcagtaata aatgcccaac
    20341 gtccgcccag cgaacttttt acccacgcgt agacaccggc ttctgagttt ttatttaacg
    20401 aaacaaattc tgcgatgatc agacagaagg gaataaaata aaaaatcgtc gcgaggaaaa
    20461 acatcggtgc cgaggccagt ccaagctcaa tattattatt gataacgttg ttaaagctgt
    20521 aaacggcggc aaaagtcagc gagagcaagc cgaatttgcc gattgtatta cgtttggtat
    20581 cagacataac acatctccga aaatgtgtag ggtagcggtg gcggcaaact ccttttgccg
    20641 ccgtagttgt tatttgttat ggaaataacc atcttcgatg gtgactttga gaaccacttt
    20701 tttgaccgcg ttattgcaga taaaccgata cgcttcatgg atatcgcaaa taacgatttg
    20761 cccttcgtgg acctctacgg tttctccgca gccttttaaa tattcacggt cagtttcatc
    20821 acgataatat tccactacct gtaatgtctc tttcggcgca tattcaattt tttgctgccc
    20881 ttgcaggtag taatgcactt caaaatagcg tcgatgcccg gtaaatagcg catgggtcgc
    20941 ggagtcattt tccacacggt aggtcaatga atcaccgatg gagtgggcga cgccaggctg
    21001 aatgttgtcg atattttcaa tcgcttcaac gcagcgttgc cacttcttgc cagaggcgta
    21061 aatctggcgg aactgttcta agttatcgat gatcctcatt gcttattctc gctgtgcaaa
    21121 ttcgtggaaa agaaccctgc gccgaactca tacgacgcca ggctttgcgc ggtagcttct
    21181 ccgccagaaa ccggcagcaa cgtaaagccg tagctgaagt cacggaacca gacgcgccag
    21241 gagtccagca cctcgctgcc ccaggagttg gagccgaggc caagtagctg gtggtcgagg
    21301 ttcagggtga tgtcatcact gcgctgcagc tcgttacagt gctgggcagc gtggatgttt
    21361 tcctgggtat agtgccaggc gctgaagtta attgggcgct gcggaaccac cagcagaccg
    21421 ttgtcgtggc ggttagtcag tgccgtccag cggacatgct gacggttgcc gttgttctgc
    21481 gggaagggat agttctcgaa catggcatcg acggtgctgc gccagatatc gatgatgtta
    21541 gcctgctggc tgtcggcgta gttttcgccc ggtccacgac cgtaatacgc cacctgatcg
    21601 tattcgccgt taatccccat ggtgaaaccg atgcacggaa tgatgtgcgg atagtcgccg
    21661 taacgctcgc cggaaagcgc cacgttaacc tggccatcgg cagcgatgcg ccagatgtag
    21721 gtacagcgca tcccgaagtc aaacaccggc ggtgcaataa ctgtgcggct gatgatcaac
    21781 acttcaccat cgctctgttc tacggcaaag tcgcgcagat gttcctgcat gatctgcaaa
    21841 tgattcggct gccacaaccc ttcgtactcc tgcttgtggt tgtcgatcat cggcttgaag
    21901 aagttgatct ttggctcgcg ggtcagcagc gactcgccat ttacctgcca ggatgtcggt
    21961 ttgccactca tttttgagaa ggtgatcgcg aagttgtagc cgcgaacggt gcagctcaaa
    22021 cgatcgtctt ccagcgtcag cggacgcgca ttatttggtg cgaaaggcac tggctgcgcg
    22081 gtgttttcct tcagcgggaa ctgataagtg gcgatagaat gcccggcttc gctgtagcgg
    22141 gtgcgggaat ctttggtcac cgtaatgttg aggaacgctt cgcgggcgtc cagctgcggc
    22201 agcgtgatct gcaagggggc ttcgctgttc ggcgcaacgt cgcgcagttt aatctgctgc
    22261 gtcgcgagcg tttcaccttc ggcgcgcacc tctgcgtgca gggtgtagtc atcaagcgtg
    22321 gtaaaccaca gtttattttc gactttcaac tcgccgcgag tcagcgcgtg gatttttacc
    22381 ggcgcgataa cctgtttgta ctctttcagg cccgatcccg gcgtctgatc ggaatagatc
    22441 aaaccatcaa gacagaagtt atagttgttg ggatagtcgc cgtagtcgcc gccgaattta
    22501 taccagacat tgccgtggtc gtcctgtgcc tggatcccgt ggtcgcacca ctcccagaca
    22561 taatgaccct gaatgcaatc gtgcttatag aagacgttct ggtactccgt cagcccgccc
    22621 ggtccgttcc ccatcgcatg agcatattca cagatgatgc gcggcttcgg atgcgggtat
    22681 tcaccaaact cattcatcag cggcacgcgg gtgtacatgg tggaaataat atcgaccact
    22741 tcagcatcgc gatcttcttc gtaatgcacc agtcgcgtgt catccagcgc tttcgccgca
    22801 tggtacatcg cgcggatgtt acagccatag ccggattcat tgcccagcga ccagatgatg
    22861 atcgacggat ggtttttctg tgcgtggata tggcgaacaa tgcgctcgac gtagactttt
    22921 tcccactgcg gatcgtcggt aatacggctg atatcgccga cattagcaaa gccgtgcgat
    22981 tcgacgtcgg tttccgccat cacaaacagg ccgtagatat cacacaattc gtaaaaacgc
    23041 ggatcgttcg ggtagtgagc ggtacgcacg gagttgatat tgtgctgctt catcaactgg
    23101 agatctttct cgacgcgatc cattccaacg gcgcggcctt tgcgatgatc gttgtcgtga
    23161 cggttgacgc cgtgcagcat cacataacgg ttattgatcc agaacagacc gtcgcgcact
    23221 ttgatatcac ggaagccaac gcgttgtggc accacttcca gaacgttgcc gttggcgtct
    23281 ttcagcgtca tgaccagatg gtaaagataa ggggattctg ctgaccattg ctgcggctgt
    23341 tcgacagtaa aagcaaagct ggcgctggtc agtttttcaa ttgccaaatg atcaatggcg
    23401 ctgctgtgca ccacgcgttc gccatcaaac agggtatatt ccagcgtcgt gacgacaggg
    23461 gaggcggcga gattttccag caccacttcg caggaaagcg tggcatcgca ataggcttcg
    23521 tcaaagtcgg tacgcacagt gaaatcgtta atatgcgtta ggtgttttcc gaccagataa
    23581 acatcgcgga agatccccgc cgaccaccac atatcctggt cttccacgta ggtagagtcc
    23641 gcccactgca tcacgcgcac acacaacagg ttgtcgccgg ttttaaccat cgcgctgatg
    23701 tcaaactctg cggtcaggcg actgcccttg ctgaaaccca catactgacc gttaacgtag
    23761 acttcaaaat aggtttcgac gccgtcaaat ttaatcagcg tctgtttacc ctgccagccg
    23821 tcgctgaggg tgaaaatacg ttgataggca ccggttgggt tatcgctggg gacaaacggc
    23881 acatcgatgg ggaacggaaa accttcgtcg gtatattgca gtttgccgtg accttccatt
    23941 tgccacatgg cggggacggt aatatgcccc cagtcagcca ttaactcaga ggtgaaggct
    24001 tctggtactt gcagcggatg gtcaaaaaag tggaaattcc actgaccgct taagggcaga
    24061 aacaggctgc tggtttcgcg ggcaaaggta cgcgcttgcg caacagaatc atatgaaaaa
    24121 aagtacgcac gcggcgcaag tcggttttcg tgggtgagct gaatgttttc ccagcgattc
    24181 ataggacctc ccggtagaga gaaatcggaa ccattcatgg tgatggtagt ttgtgtaatt
    24241 gctggggggg gtagtaaaat ttttactaaa agtagcaggt aaatgcattt actttaactg
    24301 gatcacgaaa ataaacggca gcactgtccg gcaaaaatga ttgccggaca gtgtgtaagg
    24361 ggatttaacg ggtcgtgccg cgcagtttta atttgctggg aacgaagact agcagcggca
    24421 gcgcgcgacc atcgcgggct ttttcataca ccaggttaac gccctgactt cccatcatct
    24481 cggaatggat gcgcacggtg gagagcggcg gaaaggtaaa tcgcgcggtg gggatatcgt
    24541 taacgctgat aagcgaaata tcttgtggga tgttcaggcc acgttcatga attgcccgca
    24601 gtacgccgat agcaatggaa tcggaagcaa caaacagtgc cttcggatag tcttcccgcg
    24661 ccagcatttg ttttgccagt tcataacccg acgaactgga aaaaccgccg cgccagatat
    24721 cctcttcgcg taccacttgt ttcagtcggc catattccgc aaaggcgacc tcacgaatat
    24781 ccgccttgcc aggctcatct tcaccgccaa taaaaccaat acgattaacg ccctggttga
    24841 tatagaagtc gatgatttct ttactgatgc gtgccagatc gatatccacc gcatcgtaac
    24901 cgctgccggg ttcgtgaaag tcgataaaac agatattgtc ggtcaacgcg ctggcagcgg
    24961 cgcgcagggc gggcgtgggt ttgccgacaa ttaaaatacc ggtgacgttt ttaatgtctg
    25021 gtaatccgtt gtgttcataa cagttggtca gttcgatcgc cagcttttcg cactgagttt
    25081 caatgccgtg gcggatcgcc agatagtaag gatcgttgat ctccagctcc tgctggtagc
    25141 tgtagatagc cagaatatgg tgttggttga ctgcgcctgt ctgtagttta cgggcgctac
    25201 tggtcttgta ctccagcttt tcggcgatct cgagaatgcg atgtttcgtc tcttctttca
    25261 cattcaatgt cggatcgtca tttaagaccc tggatactgt cgccagggat acgccagctt
    25321 cgattgcgat gtcttttaat gttgccatgt ttaccttttt tactttccct gatgtgcgcc
    25381 cccattgtag aggaagtgct tatcgggtca cctgttttca gtaaataaag cgcgtttctt
    25441 tgacgctgta acggtaacac agcggcttag gaatgtgatc acgcctgcat tctccatttt
    25501 tagcctacag gattgagaaa aacttttgct atagtttagt aaaattttac ccgaagggag
    25561 gaagcaatgg aaaccgtggc ttacgctgat tttgcacgtc tggaaatgcg cgtcggaaag
    25621 attgtggaag tgaaacgcca tgaaaacgcc gacaagctgt acatcgtaca ggttgatgtg
    25681 gggaaaaaac actgcaaacc gtgaccagct tggtgccgta ctacagcgaa gaggagctga
    25741 tggggaaaac ggtggtggtc ttatgcaatc tgcaaaaagc gaaaatgcgt ggtgaaacgt
    25801 cggaatgcat gctgttgtgt gcggaaaccg atgacggcag tgaaagcgtg ttgttaacgc
    25861 cggagcggat gatgcctgcg ggcgtacgcg tcgtataaaa agatcggatg gcgacgtcgt
    25921 atcgccatcc gatttgatat tacgcttctt cgacacttac tcgcatggcc gccagcgcct
    25981 tacgcgccgc tttgatcacc agttcacact gttcaatggt cagtgtcagt ggcggttcaa
    26041 tgcggatcgt tttggcgtta ttgagcgttc cggccaccag tacgcgctgg cggaacatct
    26101 cgctggcaaa gttatagccg atttcgttat caacaaactc aatcgccatc aacatccctt
    26161 taccacgcgc ttcctgtacc agatcgggat attcccgcgc cagttgacgg aaaccgtcca
    26221 gcaacatatc gcctttttgc tcagcctgag ccggtaagtt ctgctccagc aacacattga
    26281 tggtcgccag cgccgccgca caggccagcg ggttgccgcc aaaggtggtg gtatgcagga
    26341 atgggttgtc gaacagaact gaaaacacct cttcagtggc gatggtcgcg ccaatcggca
    26401 tcacgccgcc gccgagggct tttgccagac acagaatgtc cggctgaacg ttctcatgtt
    26461 cgcaggcaaa catcttgccc gtgcgcccca tgcccgtttg tacttcatcg aggatcatca
    26521 gtgcgccgaa ctcatcgcac agcttacgca cggcggtgag atagcccggc ggcggcagaa
    26581 ttacgccacc ttcgccctga atcggttcga ggatcaccgc agccacatca tcaccggttt
    26641 ttttgcactc attaagagcc gtgcgcatgg cttcgatatt gccaaacggc acatgacgga
    26701 accctggcag cagtggcata aacggtttgc ggaaggtcga tttcgccgtg gccgacagcg
    26761 cgccaagtga tttaccgtgg aacgcgccgc tggtggcaat aaaagtaaat ttgccgcgcg
    26821 gcgactgata agccttcgcc agcttcagcg ccgcttcaac ggactcggtg ccgctattac
    26881 agaagaagct gtatttcagt ttaccgggcg ttagcgcagc aagggttttc gccagcatag
    26941 cccgtagcgg atcgagtagc tcctggctgt gcagtggttg tttcgcaagt tgattctgta
    27001 cggcggaaac cacaactgga ttacggtgcc ccacgttgaa aattccaaaa cctcccaggc
    27061 agtcgataaa ctcctgtcct tgggtgtcga caagcgtatt taaacctccc gcttgccact
    27121 ctacggctcc gtaatccccg ccggcggtaa cagatttgcg atactctaaa aaccccggat
    27181 tgacatgctc tttgaagtat tcaatcacct ctcggttaag tgctttcatc tcctcatgat
    27241 ccagcgttcg cttctcaatg agattcaggg cgtgggcgct gcacgctaaa gccgatgcgc
    27301 tcgaaggtaa cctgttcaaa atatgctccg gaggctcgcg tatcacatga taccgattta
    27361 agtattgcag ggattgcgcc actccggctg cgatcgcgaa aatcgggata aacacaaatg
    27421 cggttatcat ttgtgcaata tttaatgcta tccgttaaca tttgttgtta ttttgcacgg
    27481 ttttgtcggg tatggatttg atgattgctg cgcaaatgca cgttttcagg gcaataattg
    27541 cctgattttt gggcaagata attaacgttt tcgtatagta acagagtgaa agtattttaa
    27601 atcaaagaat tagatttgtg ctttaatcgc aaattgcgat ctaaatcaaa ttaatcggtt
    27661 aaagataacc gcagcggggc cgacataaac tctgacaaga agttaacaac catataacct
    27721 gcacaggacg cgaacatgtc ttctcatccg tatgtcaccc agcaaaatac gccgctggcg
    27781 gacgatacca ctctgatgtc cactaccgat ctgcaaagct atatcactca tgctaatgac
    27841 acttttgtgc aggtgagcgg ctttaccttg caagagttac aagggcagcc gcacaacatg
    27901 gtgcgtcacc cggatatgcc aaaagcggcg tttgcggata tgtggttcac cctgaaaaaa
    27961 ggggagccct ggagcggcat cgtgaaaaat cgccgcaaaa atggcgacca ttattgggtg
    28021 cgggccaatg cggtaccgat ggtgcgcgag ggaaaaatca gtggctatat gtcgattcgt
    28081 acccgggcga cggatgaaga gattgcggcg gtggagccgc tgtacaaagc gttgaactcc
    28141 ggacgtacca gtaagcgtat tcataaaggc ctggtggtgc gtaaaggctg gctgggtaaa
    28201 ctgccttcat taccgcttcg ctggcggacg cgtggagtga tgaccctgat gtttatcttg
    28261 ctggcggcca tgctttggtt tgttgctgcc ccggtggtga cgtatttcct ctgtgtgtta
    28321 gtggtattgt tggcaagcgc ttgttttgaa tggcagattg tgcgcccgat agaaaatgtc
    28381 gcccgtcagg cactgaaggt ggcgaccgga gagcgtaata gtgttgagca cctgaatcgc
    28441 agcgatgagc tggggctgac attacgcgcg gtagggcagc ttggcctgat gtgccgttgg
    28501 ctaattaacg atgtctcaag ccaggtgtcc agtgtcagaa atggcagtga gacgctggcg
    28561 aaaggcaccg atgaactgaa cgaacatacc cagcagacag ttgataacgt tcagcaaacg
    28621 gtggcgacca tgaaccaaat ggcggcgtcg gtgaaacaga actctgccac ggcgtcggct
    28681 gccgataaac tttctatcac cgccagtaat gcggcagtgc agggtgggga ggcgatgacc
    28741 acggtgatca agacaatgga cgatatcgcc gacagtaccc agcgcattgg caccattact
    28801 tcgctgatta acgatattgc gtttcagacc aatattctgg ccctgaatgc ggcggtggaa
    28861 gcggcgcgtg ccggcgaaca gggcaaaggt tttgcagtgg tggcagggga agtgcgtcat
    28921 ttagccagcc gcagcgctaa tgctgccaac gatattcgca agctgattga tgccagtgct
    28981 gataaggtgc aatccggttc gcagcaggta cacgccgccg gacggacgat ggaagatatt
    29041 gtggcacagg tgaaaaacgt cacccagttg atcgcccaga ttagccattc aacgctggaa
    29101 caggccgatg ggctttccag cctgacccgt gcagtggatg agcttaacct gatcacccag
    29161 aaaaatgccg agctggtgga agagagtgcg caggtgtcgg cgatggtgaa acaccgcgcc
    29221 agccgactgg aagacgcggt gacggtgctg cattaatcgg tatgccggat ccggcggttg
    29281 gagcacaatg cctgatgcga tgctgacgca tcttatcagg cctgcggggt gtgtagcggg
    29341 tgtaggccgg ataaggcgcg tcaagcgtcg catccggcaa ttgcaccgcg ccactggcgg
    29401 atgcggcgat taatccagtt gcgtaatatc aaaagcggcg cggtcgatca ccgcaatgat
    29461 cttttttatt tgtgcatcac tgatatcgct ctggttgacg cgtaaatcca gcaccgcttt
    29521 aaaattatcc agcgcccgct tcatttgcgg gttctggcgc agcgccgcgc caacgcaacg
    29581 cgctttgatg cgttcttcaa tcatctccac ctgttcgcgg ttttcttcca gccactgcgc
    29641 gccttgttcg gtcagcgcaa tctgcttctt accaccttcc tcttcgcgga tggtaatcag
    29701 cgactgctcc tgcagaaaat ccagcgtcgg gtagatgacg cccgggcttg gggtgtaatt
    29761 cccctgggtt agattctcaa tcgctttaat caattcgtaa ccgtggctgt catcgcgcga
    29821 gagaatatcc agaatcacca gacgcaattc accgtgacca aagaaacgtt gccgacgacc
    29881 gccgccgcga ccgtggcgac caccgcaaca ttgaccgtgt tcatgctggt gaccgtgtcc
    29941 gcagtgctcg tgttctgact tctcaccttt gcagcagccc tcatggcgtg gctggccttc
    30001 atgtttacaa cacccttcgt gatgatggct catatcagcc tccttttgct tgattagata
    30061 tatctaattt atatctgatg tgagcattac tgcaagtaca aaaatgattt ttaaacgtgg
    30121 aagcacctca catctaggcc ggataagacg ttcacgccgc atccggcagc attgctatgg
    30181 cactgaatgc cttcacatac ttcagacata tcagttctac aaatcgcttg catttatcat
    30241 gattaacaat cattatcatt tgcgaatttt atttagatat atctgattac catcacgaag
    30301 gcgataacaa tgaataacac cccccgctac ccgcagcgcg ttcgcaatga tctgcgcttc
    30361 cgtgaactga ctgtgctacg cgttgagcgc atcagcgccg gttttcagcg cattgtcctt
    30421 ggcggcgagg cgttggacgg ttttacatcg cgcggctttg acgatcacag caaactcttc
    30481 tttcctcaac ctgacgctca ctttgtgccg ccaacggtaa cggaagaggg catcgtctgg
    30541 ccggaaggac cacgcccacc gtcgcgtgac tatacgccac tgtatgacga actacgccat
    30601 gaactggcga tcgatttctt tattcacgac ggtggggtcg ccagcggctg ggcgataaac
    30661 ttacggtggc aggtccgcgc ggttcgctgg tggtgccgga agattacgcg tatcagctgt
    30721 atgtctgcga tgaatccgga atgcccgcac tgcgccgccg cctggaaacg ttgagcaaac
    30781 ttgccgttaa accgcaagtt agtgcgctgg ttagcgtgcg ggataacgcc tgtcaggatt
    30841 atctcgcgca ccttgatggt tttaatatcg aatggctggc acatgatgag caggcggtag
    30901 atgcgcgtct ggcgcagatg caaatccctg ccagatgatt acttcatctg gataaccggc
    30961 gaaggtaaag tcgttaagaa tttaagccgc cgctttgaag cggaacagta tgacccacag
    31021 cgggtacgtg cagcggctta ctggcacgca aagtaactga tatataaaaa gaaaaaggct
    31081 gacgatttct cgtcagcctt tgctatatct ggtggcccct gctggacttg aaccagcgac
    31141 caagcgatta tgagtcgcct gctctaacca ctgagctaag gggccatggt agcggattat
    31201 aaagtaactc cgtgtcgcaa tccagccatt accgcgcgcc tgctgttttt ataaacaatg
    31261 tattttcaat ccgttatact tttcctggtg atgttagaaa ggagtaaata tggttgagga
    31321 tattttggct ccagggttac gggtcgtgtt ttgcggtatc aaccctgggc tttcatccgc
    31381 cgggactggt tttccctttg ctcatccggc aaatcgcttc tggaaggtga tatatcaggc
    31441 cgggtttacc gaccgtcagt tgaagccgca ggaggcacag catctgctgg attatcgttg
    31501 tggcgtcacc aaactggtag accgtccaac ggtgcaagcc aatgaagttt caaagcagga
    31561 gctgcacgca ggtgggcgta agctgattga aaaaattgaa gattatcagc cgcaggcgtt
    31621 ggcgattctg ggcaaacaag catatgaaca gggattcagc cagcgcggtg cacagtgggg
    31681 gaaacaaacg ctcaccattg gttcgacgca gatttgggtg ctgccaaatc ccagcggttt
    31741 aagtcgcgtt tcactggaga aactggttga agcgtatcgc gagctggacc aggcgctggt
    31801 agtgcgtggg cgataaaaaa cgccaccgaa aag
//
LOCUS       DAJTNM010000035        31711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTNM010000035 DAJTNM010000000
VERSION     DAJTNM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.035"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            70..1347
                     /gene="waaA"
                     /locus_tag="OO172_001718"
     CDS             70..1347
                     /gene="waaA"
                     /locus_tag="OO172_001718"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5511934.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1355..1834
                     /gene="coaD"
                     /locus_tag="OO172_001719"
     CDS             1355..1834
                     /gene="coaD"
                     /locus_tag="OO172_001719"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5511935.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="OO172_001720"
     CDS             complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="OO172_001720"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5511936.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="OO172_001721"
     CDS             complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="OO172_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5511937.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="OO172_001722"
     CDS             complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="OO172_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5511938.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3421..4089)
                     /gene="radC"
                     /locus_tag="OO172_001723"
                     /pseudo
     CDS             complement(3421..4089)
                     /gene="radC"
                     /locus_tag="OO172_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            4261..5481
                     /gene="coaBC"
                     /locus_tag="OO172_001724"
     CDS             4261..5481
                     /gene="coaBC"
                     /locus_tag="OO172_001724"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5511939.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5459..5917
                     /gene="dut"
                     /locus_tag="OO172_001725"
     CDS             5459..5917
                     /gene="dut"
                     /locus_tag="OO172_001725"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5511940.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6024..6620
                     /gene="slmA"
                     /locus_tag="OO172_001726"
     CDS             6024..6620
                     /gene="slmA"
                     /locus_tag="OO172_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5511941.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="OO172_001727"
     CDS             complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="OO172_001727"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5511942.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7364..8080)
                     /gene="rph"
                     /locus_tag="OO172_001728"
     CDS             complement(7364..8080)
                     /gene="rph"
                     /locus_tag="OO172_001728"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5511943.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8207..9070
                     /locus_tag="OO172_001729"
     CDS             8207..9070
                     /locus_tag="OO172_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5511944.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9291..10115
                     /gene="dinD"
                     /locus_tag="OO172_001730"
     CDS             9291..10115
                     /gene="dinD"
                     /locus_tag="OO172_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5511945.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10406..11023
                     /locus_tag="OO172_001731"
     CDS             10406..11023
                     /locus_tag="OO172_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5511946.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11020..12537)
                     /gene="ligB"
                     /locus_tag="OO172_001732"
     CDS             complement(11020..12537)
                     /gene="ligB"
                     /locus_tag="OO172_001732"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5511947.1"
                     /translation="MEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTG
                     VRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQK
                     VSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTL
                     NSLAVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLP
                     FVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVA
                     SLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLGGQGIPRIDDVVWRGAERTK
                     PTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEH
                     IFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASD
                     ERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(12559..12816)
                     /locus_tag="OO172_001733"
     CDS             complement(12559..12816)
                     /locus_tag="OO172_001733"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511948.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            12956..13579
                     /gene="gmk"
                     /locus_tag="OO172_001734"
     CDS             12956..13579
                     /gene="gmk"
                     /locus_tag="OO172_001734"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5511949.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13634..13909
                     /gene="rpoZ"
                     /locus_tag="OO172_001735"
     CDS             13634..13909
                     /gene="rpoZ"
                     /locus_tag="OO172_001735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5511950.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13928..16036
                     /gene="spoT"
                     /locus_tag="OO172_001736"
     CDS             13928..16036
                     /gene="spoT"
                     /locus_tag="OO172_001736"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5511951.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16043..16732
                     /gene="trmH"
                     /locus_tag="OO172_001737"
     CDS             16043..16732
                     /gene="trmH"
                     /locus_tag="OO172_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5511952.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16738..18819
                     /gene="recG"
                     /locus_tag="OO172_001738"
     CDS             16738..18819
                     /gene="recG"
                     /locus_tag="OO172_001738"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5511953.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18853..20058)
                     /gene="gltS"
                     /locus_tag="OO172_001739"
     CDS             complement(18853..20058)
                     /gene="gltS"
                     /locus_tag="OO172_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5511954.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20338..21729
                     /gene="xanP"
                     /locus_tag="OO172_001740"
     CDS             20338..21729
                     /gene="xanP"
                     /locus_tag="OO172_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5511955.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21850..23559
                     /locus_tag="OO172_001741"
     CDS             21850..23559
                     /locus_tag="OO172_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5511956.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23612..25930)
                     /gene="yicI"
                     /locus_tag="OO172_001742"
     CDS             complement(23612..25930)
                     /gene="yicI"
                     /locus_tag="OO172_001742"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5511957.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25940..27323)
                     /locus_tag="OO172_001743"
                     /pseudo
     CDS             complement(25940..27323)
                     /locus_tag="OO172_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27616..27710
                     /locus_tag="OO172_001744"
     tRNA            27616..27710
                     /locus_tag="OO172_001744"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27650..27652,aa:Sec,seq:tca)
     gene            28010..29194
                     /locus_tag="OO172_001745"
     CDS             28010..29194
                     /locus_tag="OO172_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5511958.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSALIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29606..30649
                     /locus_tag="OO172_001746"
     CDS             29606..30649
                     /locus_tag="OO172_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5511959.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSRIQYQ"
     gene            31164..31592
                     /locus_tag="OO172_001747"
                     /pseudo
     CDS             31164..31592
                     /locus_tag="OO172_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31622..>31711)
                     /locus_tag="OO172_001748"
     CDS             complement(31622..>31711)
                     /locus_tag="OO172_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5511960.1"
                     /translation="YIECDYNRWRRHSACGGLSPEQFENHNLA"
BASE COUNT         7957 a         8350 c         7861 g         7543 t
ORIGIN      
        1 gaaaagtaat ggtaaagcca cagctaaata catagaatcc ccagcacatc cataagtcag
       61 ctatttacta tgctcgaatt gctttacacc gcccttctct accttattca gccgctgatc
      121 tggatacggc tctgggtgcg cggacgtaag gctccggcct atcgaaaacg ctggggtgaa
      181 cgttacggtt tttaccgcca tccgctaaaa ccaggcggca ttatgctgca ctccgtctcc
      241 gtcggtgaaa ctctggcggc gatcccgttg gtgcgcgcgc tgcgtcatcg ttatcctgat
      301 ttaccgatta ccgttacaac catgacgcca accggttcgg agcgcgtaca atcggctttc
      361 gggaaggatg ttcagcacgt ttatctgccg tatgacctgc ccgatgcgct caaccgtttc
      421 ctgaataaag tcgaccccaa actggtgttg attatggaaa ccgaactatg gcctaacctg
      481 attgcggcgc tacataaacg taaaattccg ctggtgatcg ctaacgcgcg actctctgcc
      541 cgctcggccg caggttatgc caaactgggt aaattcgtcc gccgcttgct gcgtcgtatt
      601 acgctgattg ctgcgcaaaa tgaagaagat ggtgcacgtt ttgtggcgct gggcgcaaaa
      661 aataaccagg taaccgttac cggtagcctg aaattcgata tttctgtaac tccgcagttg
      721 gctgctaaag ccgtgacgct acgccgccaa tgggcaccac accgcccggt atggattgcc
      781 accagcactc acgaaggtga agagagcgtg gtcattgccg cacatcaggc attgttacag
      841 caattcccga atttattgct catcctggta ccccgtcatc cagaacgctt cccggatgcg
      901 attaaccttg tccgccaggc tggactaagc tatatcacac gctcttcagg ggaagtcccc
      961 tccaccagca cgcaggttgt ggttggcgat acgatgggcg agttgatgtt actgtacggc
     1021 attgccgatc tcgcctttgt tggcggttca ctggttgaac gtggtgggca taatccgctg
     1081 gaagctgccg cacacgctat tccggtattg atggggccgc atacttttaa ctttaaagac
     1141 atttgcgcgc ggctggagca ggcaagcggg cttattaccg ttaccgatgc cactacgctt
     1201 gcaaaagagg tttcctcttt actcaccgac gccgtttacc gtagtttcta tggccgtcat
     1261 gccgttgaag tactgtatca aaaccagggc gcgctacagc gtctgcttca actgctggaa
     1321 ccttacctgc caccgaaaac gcattgaggt tgttatgcaa aaacgggcga tttatccggg
     1381 tactttcgat cccattacca atggtcatat cgatatcgtg acgcgcgcca cgcagatgtt
     1441 cgatcacgtt attctggcga ttgccgccag ccccagtaaa aaaccgatgt ttaccctgga
     1501 agagcgtgtg gcactggcac agcaggcaac cgcgcatctg gggaacgtgg aagtggtcgg
     1561 gtttagtgat ttgatggcga acttcgcccg taatcaacac gctacggtgc tgattcgtgg
     1621 cctgcgtgcg gtggcggatt ttgaatatga aatgcagctg gcgcatatga atcgccactt
     1681 aatgccagaa ctggaaagtg tgtttctgat gccgtcgaaa gagtggtcgt ttatctcttc
     1741 atcgttggtg aaagaggtgg cgcgccatca gggcgatgtc acccatttcc tgccggagaa
     1801 tgtccatcag gcgctgatgg caaaattagc gtagcgttta tgccggatgg tatgccatcc
     1861 ggctcgcatg aattacttct ggcactgccg acaataaaac gttgcccgct gcgcatgttt
     1921 agtcgccaca atcggcgtac cgcacacccg acacggctca ccttttcgcc cgtaaacctg
     1981 caattcctgc gcgaaatagc ccggtttacc atcactttgc agaaaatctt tcagcgttgt
     2041 accaccctgc tcaatcgaac gcagcaacac cgctttaatc acccgagcta acaattcaca
     2101 ctctgccagc gacagtgatg acgccagccg atccggatgg atccccgccg caaacagtga
     2161 ttcgctggca tagatattcc ctacccctac caccagcttg ttatccatca gccacggttt
     2221 aatcgccgtt tttttcttcg cgcacttctg atgcagatac tcaccattga aatcgtcgct
     2281 aagcggctcc ggtccaagat gggccagcac attatgccct tccagctctt tggtccacag
     2341 ccaggcacca aagcggcgcg gatcggtgta gcgcagcact ttaccgttgc tcatcaccaa
     2401 atccacatgg tcatgctttt cagggggaag ttcttctgga aggatgcgca ggctgccaga
     2461 catccccaaa tgaatgataa tccagccctc aggcagctcc agcagcagat atttagcccg
     2521 ccgctgcacg ctaagcactg gttgatcgct taatcggtag atctcttctg aaaccggcca
     2581 gcgcaagcgt ccgttgcgca ccaccgcatg aagaatggtt gcaccaacga gattcggttc
     2641 tatgccgcga cggctggttt caacttcggg taattcaggc atagcatctc caggaatgaa
     2701 cagatgcaat caatatgggg gcaagcagat aacaaaaaac cccgccggag cgaggttttt
     2761 cgttacatca aagcgagaat tatttgattt tcgcttcttt gtagatcacg tgctgacgaa
     2821 caactggatc gaattttttc agttccagtt tttccggctt agtacgtttg ttcttcgtag
     2881 tggtatagaa gtgaccagta ccagcagaag aaaccagctt gattttctca cgaatacctt
     2941 tagccatgat ttatttcctc taagtactta gtacttttcg ccacgggcac gcagttcagc
     3001 cagaactgta tcgatgcctt ttttatcgat tacacgcata cctttagcag atacacgcag
     3061 ggtgacaaaa cgcttctcgc tctcaaccca gaaacggtga gagtgcaggt tcggcaggaa
     3121 acggcgttta gtcgcgttca gtgcgtggga acggttgtta ccggtcaccg gacgcttgcc
     3181 agtaacttgg cagactcggg acatgtctat tctccaaaaa tcaaattagc tcgagcttcg
     3241 tatggggtat tggcgcctcg tcaggcttta cagcccggtc atcgcagttc tatgtgaact
     3301 ctcgattgcc aggcccaaat gccaaacccg agattctcaa aggtggcgta gtatacgctg
     3361 actcagcgat gtgctcaagt cccgaacaga caaagatccc gaaggatcgc gcatagcggg
     3421 ttaaatccag ccgcgttcgg caaaagaaac atactctcca cgcccaatca cgatatggtc
     3481 gagcacgcgt aaatccatga actgacaact ctttattatc cgttcagtaa tgagtttatc
     3541 cgctttactg ggttcagcac aacccgaagg gtgattatgt gcaaggatca gcgccgaggc
     3601 gtttattttt atcgcttagc ggataatttc ccgaggatgg acttcaacat ggtttagcgt
     3661 gccggaaaaa agacggctat gcgttataac ccggtgttgt gagtcgagaa agatcaccat
     3721 aaagatctcc cgctcctcac ccgtgagctg gctttgtaaa aattcccgcg tcatctccgg
     3781 gctgagtaaa gggctttctt cacgcatccg cacgttgtag taacgtcgcg ccagttcagc
     3841 aatccccttt aactgggcaa atttcgctac gccaattcca tgaacaccac taaattgttc
     3901 atattcagag gttaacaagc cataaagaga gccgaaattc tccagcatct cttttgccag
     3961 ggttaataca tctttaccgc gcgttccggt acgcagaaat agcgccagca gctcgacatc
     4021 cgttaaggcg ctaataccaa acttcagcat tttttcgcgc ggcatcaaca gctgtgcatt
     4081 gtttttcacc ttcacctcct ttgtggtgct cgcatcctgt catagccgtt ccgttaaatc
     4141 gacggccagt tttcaatcct ggaaagagcc tcgcaaagtg aatctcaggt gaaggcacga
     4201 caacaaaagt attgtgataa aatcgccaac ttctggtgtc acccaacagg aaaaatcatc
     4261 atgagcctgg ccggtaaaaa aatcgttctc ggcgttagcg gcggtattgc tgcctataaa
     4321 acccctgaac tggtgcgtcg tttgcgcgat cgcggggccg acgtccgcgt agccatgacc
     4381 gaagcggcaa aagcctttat caccccactt agcttgcagg cggtttctgg ttatcccgtt
     4441 tccgacagtc tgcttgaccc ggcagccgaa gcagctatgg gccatattga gctgggtaaa
     4501 tgggctgatt tagtgattct cgcccctgcc acggcagatt tgattgcccg tgttgctgcc
     4561 ggaatggcga atgacctggt atcgacgatt tgtctggcta cacctgcgcc tgtagccgtg
     4621 ctccccgcca tgaaccagca gatgtaccgt gccgctgcca cgcagcataa tttagaggtg
     4681 cttgcttccc gtggtttgct catctggggg ccagacagtg gcagtcaggc ttgtggtgat
     4741 atcggtcctg gacgaatgct cgatccgtta accattgtgg atatggcggt ggcgcatttt
     4801 tcgcccgtca acgacctgaa acatctgaac attatgatta ccgccggtcc gacgcgtgaa
     4861 ccgctcgatc cggtgcgtta tatctctaat cacagctccg gcaagatggg ttttgctatc
     4921 gccgccgctg ctgcccgtcg tggcgcgaac gtcacgctgg tatcaggtcc agtttcacta
     4981 ccgacgccac cgtttgttaa acgtgttgat gtgatgaccg cgctggaaat ggaagccgcc
     5041 gtgaatgctt ctgtacagca gcaaaatatt tttatcggtt gcgccgccgt ggcggattat
     5101 cgcgcagcta ccgtggcccc agaaaaaatc aaaaagcagg ccacgcaggg tgatgaatta
     5161 acaataaaaa tggttaaaaa ccccgatatc gtcgcaggcg ttgccgcact aaaagaccat
     5221 cgaccctacg tcgttggatt tgccgccgaa acaaataatg tggaagaata cgcccggcaa
     5281 aaacgtatcc gtaaaaacct tgatctgatc tgcgcgaacg atgtttccca gccaactcaa
     5341 ggatttaaca gcgacaacaa cgcattacac cttttctggc aggacggaga taaagtctta
     5401 ccgcttgagc gcaaagagct ccttggccaa ttattactcg acgagatcgt gacccgttat
     5461 gatgaaaaaa atcgacgtta agattctgga cccgcgcgtt gggaaggaat ttccgctccc
     5521 gacttatgcc acctctggct ctgccggact tgacctgcgt gcctgtctcg acgacgccgt
     5581 agaactggct ccgggtgaca ctacgctggt tccgaccggg ctggcgattc atattgccga
     5641 tccttcactg gcggcaatga tgctgccgcg ctccggattg ggacataagc acggtatcgt
     5701 gcttggtaac ctggtaggat tgatcgattc cgactatcag ggccagttga tgatttccgt
     5761 gtggaaccgt ggtcaggaca gcttcaccat tcaacctggc gaacgcatcg cccagatgat
     5821 ttttgttccg gtagtacagg ctgaatttaa tctggtggaa gatttcgacg ccaccgaccg
     5881 cggtgaaggc ggctttggtc actctggtcg tcagtaacac atacgcatcc gaataacgtc
     5941 ataacatagc cgcaaacatt tcgtttgcgg tcatagcgtg ggtgccgcct ggcaagtgct
     6001 tattttcagg ggtattttgt aacatggcag aaaaacaaac tgcgaaaagg aaccgtcgcg
     6061 aggaaatact tcagtctctg gcgctgatgc tggaatccag cgatggaagc caacgtatca
     6121 cgacggcaaa actggccgcc tctgtcggcg tttccgaagc ggcactgtat cgccacttcc
     6181 ccagtaagac ccgcatgttc gatagcctga ttgagtttat cgaagatagc ctgattactc
     6241 gcatcaacct gattctgaaa gatgagaaag acaccacagc gcgcctgcgt ctgattgtgt
     6301 tgctgcttct cggttttggt gagcgtaatc ctggcctgac ccgcatcctc actggtcatg
     6361 cgctaatgtt tgaacaggat cgcctgcaag ggcgcatcaa ccagctgttc gagcgtattg
     6421 aagcgcagct gcgccaggta ttgcgtgaaa agagaatgcg tgagggtgaa ggttacacca
     6481 ccgatgaaac cctgctggca agccagatcc tggccttctg tgaaggtatg ctgtcacgtt
     6541 ttgtccgcag cgaatttaaa taccgcccga cggatgattt tgacgcccgc tggccgctaa
     6601 ttgccgcgca gttgcagtaa tatgacgccg gatgactttt catccggcga gtttctttaa
     6661 acgccaaact cttcgcgata ggccttaacc gccgccagat gttccgccat ttccggcttc
     6721 tcttccagat aggcaatcag atctttcagg gtgatgatag agatcacttt gcagttgtaa
     6781 tcacgctcaa cttcctgaat cgccgaaatc tcgccgcgcc cgcgttcctg acgatcgagc
     6841 gaaatcaaca cgccagcaag cgtcgcgcca ttggcctgaa taatctccat cgactcgcga
     6901 atcgccgttc cggcggtgat cacatcatct accagcatta cgcgtccttg taacgcgcta
     6961 ccaaccagat tgccgccttc accgtggtct tttgcttctt tgcggttaaa gcagtacggc
     7021 aggtccaggt cgtgatgctc cgccagtgcc acagcggttg tggtggcaat cgggatccct
     7081 ttgtaagcag ggccaaacag cagatcgaac tcaatgccgg aatccaccaa cgcttcagcg
     7141 taaaaacggc ctaacagtgc cagatcgcgc ccggtattaa acagcccggc gttgaagaaa
     7201 taggggcttt tgcgcccgga tttcagcgta aactcgccaa actttaacac ctgcttgcta
     7261 agcgcaaatt caataaactg gcgctgatat ggtttcatgc cttcgctcct catcttactt
     7321 ttctacagac aaaaaaaagg cgactcatca gtcgccttaa aaatcagttt gccagcgccg
     7381 ccttctgcgt cgctacaatg gattcgattc cccctcgggc cagcgccaac aaggtgagta
     7441 gctcttcatg ggtgaacggc tcgccttctg ccgtcccctg cacttcaatg atgcgcccgt
     7501 cttcggtcat cactacgttc atgtcggtct ctgcggctga gtcttcaacg tattccagat
     7561 cgcaaattgc ttcaccgttt acaataccga cagaaactgc ggctaccatc cctttcatcg
     7621 ggttggtttt cagcttgccg ttttccacca gcttctgtag cgcatctgcc agcgccacgc
     7681 aggcacccgt aatcgacgcg gtacgagtgc caccatcagc ctgaagcacg tcgcagtcca
     7741 gcgtaatggt gaactcaccc agcgctttca aatctactgc cgcgcgaaga gcacgggcga
     7801 tcagacgctg gatttccatt gtgcgtccac cctgcttacc tttcgccgct tcacgagcgt
     7861 tacgggtgtg ggtagaacgt ggcagcatgc cgtactctgc ggtgatccag ccctggccct
     7921 gacctttcag gaagcgcggc acgccttctt caatagaggc ggtacacaat actttggtat
     7981 cgccaaattc gaccagcacc gagccttctg catgttttgt atagttacga gtcagggtaa
     8041 cgggacgcac ctgattattg ctacggcctg ctggacgcat attgaaatct ccggcttgaa
     8101 acaaatgtgg ctgcgcatta tacggacttc cggcggttat tcctatcctg acaaggcatc
     8161 gatggctata atccttccac ctctcctttt ataaacagga acgtctatga tccgcagtat
     8221 gaccgcctac gcccggcgtg aaatcaaggg tgaatggggg agcgcaacct gggaaatgcg
     8281 ctcggtaaac cagcgttatc tggaaactta ctttcgtctg ccggagcagt tccgtagcct
     8341 tgaacctgtc gttcgcgagc gtattcgttc tcgcctgacg cgcggtaaag tggaatgtac
     8401 cctgcgctat gagccagatg ttagcgcaca aggtgagctg atcctcaatg aaaaactggc
     8461 taaacagctg gtaactgccg cgaactgggt aaaaatgcag agtgacgaag gggaaatcaa
     8521 cccggttgat attctacgct ggccgggcgt gatggcagcc caggagcagg atctcgacgc
     8581 gattgccgct gaaattctcg cggcgttgga tggtacgctg gacgacttta tcgtcgcgcg
     8641 cgaaaccgaa ggtcaggcac tgaaagcatt gatcgagcag cgtctggaag gcgtcaccgc
     8701 cgaagtggtc aaagtccgcg cccatatgcc ggaaattctg caatggcagc gtgagcgtct
     8761 ggtcgcgaag ctggaagatg ctcaggttca gttggaaaac aaccgtctgg agcaggagtt
     8821 ggttctgctg gcacaacgaa ttgacgttgc cgaagagctg gaccgcctcg aagcgcatgt
     8881 taaagagacg tacaacattc tgaagaaaaa agaagcggtt ggtcgtcgtc tggactttat
     8941 gatgcaggag ttcaaccgtg agtcgaacac tcttgcgtcg aaatctatca atgccgaagt
     9001 gacaaactcc gccatcgagc tgaaagtgtt gattgagcag atgcgcgagc agattcagaa
     9061 tattgagtaa ctcgtaacac caattctgac ttttcagcct tcacaaatgc cgcccactca
     9121 aacagagcgg catttttctt ccccgcaact tcaattctgt tttgcgtgcc tgctccagat
     9181 tttgcgatgt ttttttgccc agcacactga gaacgtgaga tactcacaac tgtatataaa
     9241 tacagttaca gatttacttt ctttgcaatc gatatcacat ggagtgggca atgaacgaac
     9301 atcatcaacc ttttgaagag ataaaactga ttaatgcaaa cggagcagaa caatggtcag
     9361 caagacaact tgggaaacta ctgggttatt cagagtatcg tcactttata cctgtattaa
     9421 cgcgcgccaa agaggcctgt gagaacagtg gtcacacaat tgatgaccat ttcgaggaga
     9481 tcctcgatat ggtcaaaatt ggctcaaatg ccaaaagagc attaaaagac atcgtactct
     9541 cccgctatgc ctgttacctg gtagtacaaa acggcgaccc cgcgaaaccg gtcattgcgg
     9601 cagggcagac ttattttact atccagaccc gacggcagga gcttgctgac gatgaagcat
     9661 ttaagcaact tcgtgaagac gaaaagcgtc tgtttctgag aaatgagttg aaagaacata
     9721 acaaacaatt ggttgaggca gcacagcaag ctggggtagc gacagctact gatttcgcca
     9781 tatttcagaa tcatggttac caggggctgt atggtggatt agatcagaaa gccatccacc
     9841 aactgaaggg gctgaaaaag agtcagaaga tcctggatca tatgggttca acagaactgg
     9901 cggctaatct ctttcgagct acccaaacag aagaaaaact caagcgggat agcgtgaatt
     9961 caaaacagca agctaatacc acacactttg acgtgggtag caaggtgagg caaaccattc
    10021 aggaacttgg cggaaccatg cctgaggagt tgccgacccc gcaggtcagc atcaagcagt
    10081 tggaaaacag tgtaaaaatt acagagaaga aatagcagaa acgaaaaact ctttttgttg
    10141 attgagacac ccgatgcgta agccaaggtc caggtgcagt gaacacaatg gctaaacatt
    10201 gcacctttct tttcccccct cagttttaac ctattttttc ttatgcattt tctcagacaa
    10261 gaagtcagaa gaatgcatct acgctacaga aaatagcgat ttcacataac taaaagttat
    10321 caatttcctc tcaccttaaa aaatatcaat cgtgacaatg cgcacaaatc gctaccctgc
    10381 cagacagatt tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcattac
    10441 tgccgaagcc atgacggggg cgctggcggc cgggcgacgg cgcatggata catttggcgt
    10501 aattattatt gcaaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg
    10561 ccactatccg ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc
    10621 agtactcacc actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct
    10681 cgatgcgctc ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg
    10741 gcacggccca attattgccg ttgtcgcggc ggtaaccacc ggtgtatttg gcggcgtttt
    10801 gcgcgatatg ttctgtaaac gcattccgct ggtctttcaa aaagagttgt atgccggtgt
    10861 ctcatttgcc tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt
    10921 ggtgattatc tccaccctgg tattcggctt ttttgctcgt ttactggcgc tacgtcttaa
    10981 gctgggatta ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat
    11041 ctgctgggca gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat
    11101 aacctgtctg gcgcgcccag atccagtccc cggcagctgc tgccagaact gctccgtgct
    11161 aaataaaagt tgcgaccagg atcgttcatc actggcatta agcgccgccc gggttagcgg
    11221 tattcccatt gccatcaccc agcgagtaaa aggctggtta cgagccagat taaactgatg
    11281 ccataactgc gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt
    11341 taataaaagc caggaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc
    11401 ggcctcacca atgccatcca gcccgagaac ctgttttgac cccagccaga ctaagcgtga
    11461 aatgaactgt tcctgacaaa catcagaagc aaagtagcag gtcaacgagt taaagcggtt
    11521 ttctggtggt gtcggttttg tacgttctgc accgcgccag accacatcat caatgcgagg
    11581 aataccctga ccgccaaggc tgacgaggat ctgatcacca ggcgcaatat cccactcctg
    11641 ccagcgcctg acggaaccaa tattcacccg ctggactttt ttatcatcca gcatgacagg
    11701 tgcgagtgac gcaaccaccg atattttacc gctcttaccc accgcaaact gaatcgcctt
    11761 cacttcggca acctgagcta caggttgata tttccaggcc accagccact ccgcctggcc
    11821 cggtagccaa tgacgggatt cgggttcctt agcccctcgc acaaccacgc catcggtgac
    11881 gaagggtaat ttcgctttcc accactcatt gcgtacgcgc gcaacctcat cagcattttt
    11941 aaccgcacgg gtatacctct gcgttagagt aaaacctgcg gtagccagct cctttaaacg
    12001 atcggtcatt aactgcggtc catccggcca tgcccagaca aaaacagcca gtgagtttag
    12061 cgtgtcgcta ttcccctggc gcatcatcag gccagcaact tttgcgcggg catttattcc
    12121 ccccatttgt tgttggatat gcccctcgcg ctggagaaat atttccccct gaagcgtact
    12181 attggctaaa ggcccgctaa cggtttgcgg cacagcggaa attaagctaa ctttctgcgt
    12241 ccagtcctcg cctttcaggc cattaccgcg actgattgct ttgttcagtt tcccgtcccg
    12301 ataaaccagg gttaccgcaa cgccatcaac ttttggctgc acccaaagat cactacgttc
    12361 tcgcatccac agacttaatg cgtttttatc cgccattttg cgtacacccg tatgggcaac
    12421 cggatgcata accgcaccat ttaaaggcgg catcatcacg tcgcggggct cgctcacaaa
    12481 gcagcgttgc cactgcgtaa gacgggcgct taactgatcg taaacaccgt cttccacctc
    12541 actttttcct tccagtagtc atcgtcccac tgttttattt gctgttgcag gcgggaaatt
    12601 tcttcctgtg ctctggctgg cgaccaggcc ggacagaccg cccacacaga tgattgccag
    12661 cacaagatac ttattaatat cgccatccat actttcatct tcacctccgc tgtagtcagg
    12721 ctggcagata tacaacgtgg tgaaggcaaa gccgagtggc aaaaacggag tctgcgagga
    12781 cgcttcctga gaatcgtctt tattgcagta aatcacaggc aaatgcggaa acagctacgc
    12841 aaaacgcaac aactttgcgc aaaaagtgtg agcaagggct acgtcacatg gccgcgccgt
    12901 gtataataag ctcgtatgta ggctttattt cgctaatcac atacgaaaga tactcatggc
    12961 tcaaggcacg ctttatattg tttctgcccc cagtggcgcg ggtaaatcca gcctgattca
    13021 ggctttatta aaaacccaac cgttgtatga cacccaggtt tctgtttcac acaccacacg
    13081 ccaaccgcgt cctggtgaag tccacggtga acattatttc tttgttaatc atgatgaatt
    13141 taaagaaatg attagcagag atgcgttcct cgaacacgca gaagtttttg gtaattacta
    13201 tggcacttcg cgtgaggcca ttgagcaagt actggcgacc ggtgtcgatg tttttctcga
    13261 tatcgactgg cagggcgcgc agcaaattcg ccagaagatg ccgcacgcgc ggagtatctt
    13321 tattttaccg ccgtccaaaa ttgaactgga ccgccgtcta cgcggtcgcg gtcaggacag
    13381 cgaagaggtc attgcaaagc gtatggcgca agctgttgca gaaatgagcc attacgccga
    13441 atatgattat ctgattgtga atgatgactt cgataccgcg ttgaccgatt tgaagaccat
    13501 tattcgcgcc gaacgtctgc gcatgagccg ccaaaagcag cgtcatgacg ctttaatcag
    13561 caaattgttg gcagactgaa cctgatttca gtatcatgcc cagtcatttc ttcacctgtg
    13621 gagcttttta agtatggcac gcgtaactgt tcaggacgct gtagagaaaa ttggtaaccg
    13681 ttttgacctg gtactggtcg ccgcgcgtcg cgctcgtcag atgcaggtag gcggaaagga
    13741 tccgctggta ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg aaatcgaaga
    13801 aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc caggaacagc aagagcagga
    13861 agccgctgaa ttacaagccg ttaccgctat tgctgaaggt cgtcgttaat cacaaagcgg
    13921 gtcgcccttg tatctgtttg aaagcctgaa tcaactgatt caaacctacc tgccggaaga
    13981 ccaaatcaag cgtctgcggc aggcgtatct cgttgcacgt gatgctcacg aggggcaaac
    14041 acgttcaagc ggtgaaccct atatcacgca cccggtagcg gttgcctgca ttctggccga
    14101 gatgaaactc gactatgaaa cgctgatggc ggcgctgctg catgacgtga ttgaagatac
    14161 tcccgccacc taccaggata tggaacagct ttttggtaaa agcgtcgccg agctggtaga
    14221 gggggtgtcg aaacttgata aactcaagtt ccgcgataag aaagaggcgc aggccgaaaa
    14281 ctttcgcaag atgattatgg cgatggtgca ggatatccgc gtcatcctca tcaaacttgc
    14341 cgaccgtacc cacaacatgc gcacgctggg ctcacttcgc ccggacaaac gtcgccgcat
    14401 cgcccgtgaa actctcgaaa tttacagccc gctggcgcac cgtttaggta tccaccacat
    14461 taaaaccgaa ctcgaagagc tgggttttga ggcgctgtat cccaaccgtt atcgcgtaat
    14521 caaagaagtg gtgaaagccg cgcgcggcaa ccgtaaagag atgatccaaa aaatcctctc
    14581 ggaaatcgaa gggcgtttgc aggaagcggg aattccctgc cgcgtcagtg gtcgcgagaa
    14641 gcatctttat tcgatttact gcaaaatggt gctcaaagag cagcgttttc actcaatcat
    14701 ggacatctac gctttccgcg tgatcgtcaa tgattccgac acctgttatc gcgtgctcgg
    14761 gcaaatgcat agcctgtaca agccgcgtcc gggtcgcgtg aaagactaca tcgccattcc
    14821 aaaagcgaac ggctatcagt ctttgcacac ctcgatgatc ggcccgcacg gtgtgccggt
    14881 tgaggtccag atccgtaccg aagatatgga tcagatggcg gagatgggtg ttgccgcgca
    14941 ctgggcttat aaagagcacg gcgaaaccag caccaccgcg caaatccgcg cccagcgctg
    15001 gatgcaaagc ctgctggagc tgcaacagag cgccggtagt tcgtttgaat ttatcgagag
    15061 cgttaaatcc gatctcttcc cggatgagat ttacgttttc acaccggaag ggcgaattgt
    15121 cgagctgcct gccggtgcaa cgcccgtcga cttcgcttat gcagtgcata ccgatatcgg
    15181 tcatgcctgc gtgggcgcac gcgttgaccg ccagccttac ccgctgtcgc agccgcttac
    15241 cagcggtcaa accgttgaaa tcattaccgc accgggcgct cgcccgaatg ccgcgtggct
    15301 gaactttgtc gttagctcaa aagcgcgcgc caaaattcgt cagttgctga aaaacctcaa
    15361 gcgtgatgat tctgtaagcc tgggccgtcg tctgctcaac catgctttgg gtggtagccg
    15421 taagctgaat gaaatcccgc aggaaaatat tcagcgcgag ctggatcgca tgaagctggc
    15481 aacgcttgac gatctgctgg cagaaatcgg acttggtaac gcaatgagcg tggtggtcgc
    15541 gaaaaatctg caacatgggg acgcctccat tccaccggca acccaaagcc acggacatct
    15601 gcccattaaa ggtgccgatg gcgtgctgat cacctttgcg aaatgctgcc gccctattcc
    15661 tggcgacccg attatcgccc acgtcagccc cggtaaaggt ctggtgatcc accatgaatc
    15721 ctgccgtaat atccgtggct accagaaaga gccagagaag tttatggctg tggaatggga
    15781 taaagagacg gcgcaggagt tcatcaccga aatcaaggtg gagatgttca atcatcaggg
    15841 cgcgctggca aacctgacgg cggcaattaa caccacgact tcgaatattc aaagtttgaa
    15901 tacggaagag aaagatggtc gcgtctacag cgcctttatt cgtctgaccg ctcgtgaccg
    15961 tgtgcatctg gcgaatatca tgcgcaaaat ccgcgtgatg ccagacgtga ttaaagtcac
    16021 ccgaaaccga aattaatgtt ttatgaaccc aacacgttat gcacgcatct gcgaaatgct
    16081 cgccaggcgg cagcctgatc tgaccgtctg catggagcag gtccacaaac ctcataacgt
    16141 ttctgcgatt attcgtaccg cagatgccgt tggcgtacat gaagttcacg ccgtctggcc
    16201 tggtagccgc atgcgcacca tggcttcggc agcggcgggt agtaacagct gggtacaggt
    16261 gaaaacacac cgcaccattg gcgatgccgt cgctcatctc aaaggccagg gcatgcagat
    16321 tctggcaacc catctttctg ataatgctgt cgatttccgc gaaattgatt acactcgccc
    16381 gacctgcatt ttgatgggac aggagaaaac gggcatcacg caggaagcat tggccctggc
    16441 ggatcaggac atcatcattc cgatgatcgg catggtgcag tcgctgaatg tttccgttgc
    16501 ctcagccctc attctttacg aagcccagcg tcagcggcaa aatgcaggca tgtacctgcg
    16561 tgaaaacagc atgttgccgg aagcagagca acaacgcctg ttgtttgaag gcggctatcc
    16621 ggtgctggcg aaagtcgcaa aacgcaaagg cctgccttat ccccacgtca atcagcaagg
    16681 cgagatcgaa gctgatgccg actggtgggc tactatgcag gctgcagggt aagtgccatg
    16741 aaaggtcgcc tgttagatgc tgtcccactc agttccctaa cgggcgttgg cgcagcactt
    16801 agtaacaaac tggcgaaaat caacctgcat accgtgcagg atctactctt acaccttccc
    16861 ctgcgctacg aagatcgcac ccatctctac cccatcggag aactactgcc gggcgtttat
    16921 gccacggtgg aaggcgaagt gctgaactgc aatatctcct tcggcggtcg gcggatgatg
    16981 acctgccaga tcagcgacgg ttccggcatc ctcaccatgc gctttttcaa tttcagcgcg
    17041 gcaatgaaaa atagcctggc gacgggccgc cgtgtactgg cttatggcga agcaaagcgc
    17101 ggtaaatatg gtgcggagat gatccacccg gaataccgcg tgcagggcga tctcagcacg
    17161 ccagaattac aggaaacgct cacgccggtt tatccaacaa cggaaggcgt aaagcaggcc
    17221 acgctgcgta aattaaccga ccaggcgctg gatctgctcg acacctgcgc cattgaagaa
    17281 ctcctgccgc cggaactgtc acaaggaatg atgacgctac cggaagcgtt gcgcactttg
    17341 caccgcccgc caccgacgct acagcttagc gatctggaaa ccgggcagca tccggcgcaa
    17401 cgtcgtctga ttctggaaga actgctggcg cacaacctca gcatgttagc cttacgtgcc
    17461 ggagcacagc gttttcatgc ccagccgctg agcgccaatg acacgctgaa aaataaactc
    17521 ctcgccgcct taccgttcaa gccaacgggc gcacaggcac gcgtagtggc ggagatcgag
    17581 catgatatgg cgctggatgt gccgatgatg cgcctggtac agggcgatgt aggttccggt
    17641 aaaacgctgg tcgccgccct cgccgcgctg cgtgcgattg cccacggcaa acaggtggca
    17701 ctgatggcac caaccgaatt actcgccgag cagcacgcca ataacttccg caactggttt
    17761 gcaccgctcg gtatcgaagt gggctggctc gccggtaagc agaaaggcaa agcgcgactg
    17821 tctcagcagg aagccatcgc cagcggtcag gtgcagatga ttgtcggtac acacgccatc
    17881 ttccaggaac aggtgcagtt taacggcctg gcgctggtga ttatcgacga acagcatcgt
    17941 tttggcgtgc atcagcgtct ggcattgtgg gagaaaggcc agcagcaggg cttccatccg
    18001 catcagttga tcatgaccgc cacgccgatc ccccgcacgc tggcaatgac ggcgtatgcc
    18061 gatctcgata cctcggtgat agatgagctg ccgccaggcc gcacgccagt gactacggtc
    18121 gctattcctg atacccgccg taccgacatc attgaccgcg tgcgccacgc ctgcataact
    18181 gaaggtcgtc aggcgtactg ggtttgtacg ttgattgaag agtcagagtt actggaagcg
    18241 caggcggcgg aagctacctg ggaagagttg aaactggcgc taccagaatt gaatgttggc
    18301 ctggtacacg ggcggatgaa accagccgag aaacaggcgg tgatggcgtc gtttaaacaa
    18361 ggtgagctac atctgctggt tgccactacc gttattgaag tcggtgttga tgtgcctaac
    18421 gccagcctga tgattatcga aaaccctgag cgtctgggtc tggcgcagtt acaccagctg
    18481 cgtgggcgcg taggtcgtgg cgcggtggct tctcactgcg tgctgctcta caaaacgccg
    18541 ctttctaaaa cggcgcaaat tcgcctgcaa gtgctgcgcg acagtaacga cggttttgtg
    18601 attgcgcaaa aagatctgga gattcgcggc cctggcgaat tgttaggcac gcgtcagacg
    18661 ggtaatgctg aatttaaagt ggcggattta ctgcgcgatc aggcgatgat cccggaagtt
    18721 cagcgcctgg cgcgacatat tcacgaacgt tacccacaac aggcaaaagc cttgatagaa
    18781 cgctggatgc cggagacaga acgttactcg aatgcgtaaa aggcggcggt gagaagaccg
    18841 ccgcttcatc ggttaaccgg caaaaatcgg caacattaaa tacagcttaa tcaccagcgc
    18901 attgacgata tcgataaaga acgcaccgac catcggcacc accaaaaacg ccatgtgcga
    18961 cgggccaaag cgttcagtga tcgcctgcat gttggcgatt gccgttggcg ttgcaccgag
    19021 gccaaaacca cagtgacccg cagccagcac tgccgcatcg tagtttttgc ccatcatgcg
    19081 ccaggtaacg aagatggcat acaacgccat gaagatggtc tgtaccacca gaatcgccag
    19141 catcggcagt gccagcgaag ccagctccca cagtttcagc cccatcaacg ccatcgccag
    19201 gaacaacgac aagcttacgt tacccagcac ggataccgca cgctcaaaga cgcggtaaaa
    19261 gcccattatt gacagaccgt tgctcagaat cacgccaaca aacagtacac agacgaaggt
    19321 cggcagttca aaagcagtgc cagccaaaag ttgcgcaaca attttcccca ccgtcaggca
    19381 gatagcaatc agcgcgatag tttcaatcag caccaacgag gtgatcatgc gtcccacatc
    19441 cggcttttca aacgccgtcg ggacttcctg gtcatccgga ataccgttcg gcgtggtgga
    19501 gtgtttcacc agatagcgcg ccaccggacc gccaatcaag ccgcccagca ccagaccgaa
    19561 cgttgcacag gccatagcca cttccgtcgc attggtgaag ccataacgtt caatgaacaa
    19621 tttactccac gcagcgcccg taccgtgacc gccggaaagc gtaatagaac cggccaacag
    19681 ccccatcagc ggatcaagcc ctaacaggct agccatacca atgccaatgg cattttgcat
    19741 caccaacaga ccaacaacca caatcaagaa gatgccaacc acacgcccac cggcacgcaa
    19801 actggcaatg ttggcgttca ggccaatggt ggcgaagaaa gccagcatta acggatcgcg
    19861 cagggacata tcaaagttga cttcccagcc catgcttttt ttcagtacta gtagcgccag
    19921 cgccaccaac aaaccacccg caacaggttc cggtatggtg tatttcttca aaaaggagac
    19981 ggaatggacc aacttacgcc cgagcagcaa cgtcagcgtt gcggcaacaa gcgttgctaa
    20041 agtatcgaga tgaaacatag ttactccttt gtatccgcat cactctttca tactcgtcat
    20101 acttcatcgc gcagtggcaa cttcatgaac tatgacgggt atcgagttat gctttcctgg
    20161 gtcaaattct cagctgttaa tgaacaaatg gcatggattt taagcagaaa aataccaaaa
    20221 gttatataaa aaagtgcatt tatctcattt aaatatttag atgaggatgt ggcaaacgtt
    20281 tgcttttctc tttcggtcag ctaaaatgcc cgctttgcta ccacgggatt gttttcaatg
    20341 tctgtttcca ccctcgagtc agaaaatgcg caaccggttg cgcagactca aaacagcgaa
    20401 ctgatttacc gtcttgaaga tcgtccgccg cttcctcaaa ccctgtttgc cgcctgtcag
    20461 catctgctgg cgatgttcgt tgcggtgatc acgccagcgc tattaatctg ccaggcgctg
    20521 ggtttaccgg cacaagacac gcaacacatt attagtatgt cgctgtttgc ctccggtgtg
    20581 gcatcgatta ttcaaattaa ggcctggggt ccggttggct ccgggctgtt gtctattcag
    20641 ggcaccagct tcaactttgt tgccccgctg attatgggcg gtaccgcgct gaaaaccggt
    20701 ggtgctgatg ttcctaccat gatggcggct ttgttcggca cgttgatgct ggcaagttgc
    20761 accgagatgg tgatctcccg cgttctgcat ctggcgcgcc gcattattac gccgctggtt
    20821 tctggcgttg tggtgatgat tatcggcctg tcgctaattc aggtggggct gacctccatt
    20881 ggcggcggtt acgcagccat gagcgataac accttcggcg caccgaaaaa tctgctgctg
    20941 gcaggcgtgg ttttagcctt aattatcctg cttaaccgtc aacgtaaccc ttacttacgc
    21001 gtggcctcac tggtaattgc gatggcggcc ggatatgcgc tggcgtggtt tatgggcatg
    21061 ttgccagaaa gcaacgaacc gatgacgcaa gaactgatta tggtgccaac gccgctctat
    21121 tacggtcttg gcattgaatg gagtctgctg ctgccgctga tgctggtctt tatgatcact
    21181 tcgctggaaa ccattggcga tatcacggcg acctctgacg tttccgaaca gccagtgtcc
    21241 ggtccgctgt acatgaaacg cctgaaaggc ggcgtgctgg caaacggcct gaactcgttt
    21301 gtttcggcgg tgtttaacac cttcccgaac tcctgcttcg ggcaaaacaa cggagtgatc
    21361 cagttgactg gtgttgccag ccgctatgtc ggttttgtcg tcgcgctgat gttgatcgtg
    21421 ctgggtctgt tcccggcagt gagcgatttt gtacaacaca ttccagaacc ggttctgggc
    21481 ggcgcaacgc ttgtaatgtt tggcaccatc gccgcctccg gtgtgcgtat cgtttctcgt
    21541 gagccgctga accgtcgggc gattctgatt atcgcgttgt cgctggcggt tggtctgggc
    21601 gtgtctcagc agccgctgat tttgcagttt gcccctgaat ggctgaaaaa cctgctctcc
    21661 tccgggatcg ccgcgggcgg tattactgcc atcgtgctga atctgatttt cccaccagaa
    21721 aaacagtaat tcactggcgg cggtaagata attaccgccg cagtctcacc tctttcacca
    21781 ttcccgcctt gaggattgcg cgtaaatcgt gcataactcc cttatgtgca tttcacggga
    21841 tggaagacca tgaaatttat tgggaagctg cttctctaca ttctcatcgc tctgttagtg
    21901 gtgatcgctg gcctctattt tcttctgcaa acccgctggg gagcagaaca tatcagcgca
    21961 tgggtttccg agaatagcga ctatcatctg gccttcgggg cgatggatca ccgtttttcc
    22021 gcgccatctc atatcgtgct ggagaatgtc acgtttggtc gtgatggtca gcccgcgccc
    22081 ctggtggcaa aaagtgtcga cattgcgcta agcagtcgcc aactgaccga accacgccat
    22141 gtcgatacca tcctgctgga aaacgggacg ctgaatctca ccgaccagac cgcgccgcta
    22201 ccgttcaaag ccgatcgtct gcaactgcgt gatatggcgt ttaatagccc gaatagcgaa
    22261 tggaaactga gcgcgcagcg ggtaaatggc ggcgtggttc cgtggtcacc agaagccggt
    22321 aaagtgctgg gtacgaaggc gcagattcag tttagtgccg gatcgctttc gctcaatgat
    22381 gttcctgcca ccaatgtact gattgaaggc agtattgata acgatcgcgt tacgctgact
    22441 aacctgggtg ccgacatcgc ccgcgggaca ttaaccggaa acgcgcagcg taacgccgac
    22501 ggcagctggc aagtggaaaa tctgcgcatg gcggatatcc gtctacaaag cgaaaaatcg
    22561 ctaaccgact tctttgcgcc attacgctct gtcccgtcgt tgcagattgg tcgcttggaa
    22621 gtcatcgatg ctcgtctgca aggtccggac tgggcggtga ccgacctcga tctcagcttg
    22681 cgcaacatga ccttcagtaa agatgactgg cagacacagg aaggcaaact gtcgatgaac
    22741 gccagcgagt tcatttatgg ttcgctgcat ttatttgacc cgattataaa cgcggaattt
    22801 tccccgcagg gcgtagcgct gcgccagttc accagccgct gggaaggggg tatggtcaga
    22861 acgtcaggga actggctgcg tgacggtaaa acgttgatcc ttgatgatgc ggcaattgcc
    22921 gggctggaat ataccttgcc gaaaaactgg caacagttgt ggatggaaac gacacccggt
    22981 tggttaaaca gcctgcaact gaagagattt agcgccagcc gcaatctgat cattgatatc
    23041 gaccctgact tcccgtggca gctcaccgcg ctcgatggtt acggtgccaa cctgacgctg
    23101 gttaccgatc ataaatgggg cgtctggagt ggctcggcga atctgaatgc cgccgccgcg
    23161 acattcaatc gtgttgatgt tcgtcgcccg tcgctggcgc tgaccgccaa cagcagcacg
    23221 gtgaatatca gcgaactgag tgcatttact gaaaaaggca ttctggaagc caccgcaagt
    23281 gtttcacaaa cgccacaacg tcagacccat atcagcctga atggacgcgg tgtgccggtg
    23341 aatattttgc aacaatgggg atggcctgaa ttacagttga ctggcgacgg caatattcag
    23401 cttaccgcca gtggagatat tcaggccaat gccccgctga aacctacggt tagcgggcaa
    23461 ttgcatgccg tgaacgccgc aaagcagcaa gtgactcaaa ccatgaatgc gggcgtcgtt
    23521 tccagcggtg aagttacatc gacggagccg gtgcggtaaa catcaatctc gccgggtacg
    23581 actcagacgt acccggcatt ccgtcaatag attacaacgt aattgtcagc gcattccctt
    23641 gaggcgtcac caccagcccc tgctcacttt cagcctgcga accgccttgc agaccattta
    23701 ctttcacaac attgcgcaag cacagcgtcc agttcttcgc ctcgcccgca ccagtcacag
    23761 taatcgtgtt gccagtacgt gctgctttta aagtaaagat caccgatccg tcagcagcag
    23821 gcacttcaca gacggcttca tgcccgtctt gcagattaaa gaggtggaat gccgtgcctt
    23881 cgtgccacac gtaatcggga cgttgatcgt tgttgcccag cgccagtaga gtgttatcac
    23941 gcacataaac gggcagactc aggaagccgt gctgctgttt atgccagcga ctaccgtcga
    24001 gttcatcgtt gtgccacagg tgtgtccagc gaccttccgg caggtagaac tgcacatcgc
    24061 ccgcttcagt gaacaccggc gcaaccatca cgttgtcgcc taacatgtat tgacggtcaa
    24121 ggtaatcaca agccggatcg tccgggaact ccatcatcat ggcccgcatc atcggcgtac
    24181 cccgcgcgtt cgcacgcgca gcttcacgat acagatacgg catcatgcgg catttcagtt
    24241 gcgtgaagaa gcgcaccaca tcacaggact catcatcgta ggcccacggc acacgataag
    24301 atttgctacc gtgtaaacgg ctatggctgg agagcaaacc aaacgcgcac cagcgtttgt
    24361 aaacgtgcgc cggagcggta ttttcaaagc cgccgatatc gtggctccag aagccaaaac
    24421 ctgaaaggcc aatagacaaa ccaccgcgca ggctttccgc cattgattcg tagttagcgt
    24481 aacaatcgcc accccagtgt accgggaatt tctgcgcacc gacggaggcc gagcgggcaa
    24541 acaagacagc ttcttcctca ccaacggtgt ccttgagcac gttccacacc agttcgttgt
    24601 agatgtacgc ataatggtta tgcattttct gcggatcgga accgtcaaac cactgaacat
    24661 cagttgggat acgttcgcca aagtcggtct taaagcaatc aacgcccatc gcgaccagac
    24721 ctttcagttt gtcggcgtac catttgcagg catccggatt ggtaaagtca taaatcgcca
    24781 gacctggctg ccatttatcc cactgccata gcgaaccgtc cgggcgtttg agtaaatagc
    24841 ctttctcttg taactcttta aagacggggg atttttgacc gatatagggg ttaatccaga
    24901 cgcagatttt cagccctttc gctttcaggc ggcggatcat cccttccggg tcagggaaag
    24961 tcagcgggtc ccactcaaaa tcgcaccact ggaaggcttt catccagaaa cagtcaaagt
    25021 ggaaaacatg cagcggcaga ttgcgttccg ccataccatc gataaagctg tttaccgtcg
    25081 cttcgtcgta gttggtggta aatgaagtgg ttagccacag gccgaaggac cacgggggcg
    25141 gcagcgccgg acgaccagta aagcgggtat aacgatcaag taccgctttc ggcgtcgggc
    25201 cgtcgataac aaagtattcg agatattcac tctcaacgct gaactgcact ttggagactt
    25261 tctccgatcc cacttcaaaa gagacacact ggggatgatt gaccagtacc ccataaccac
    25321 ggttagtcat gtagaacggg atatttttat acgcctgttc agtacttgtg ccgccgtccc
    25381 ggttccaggt ctctaccgtc tggccattgc gcaccagggc agtaaagcgc tctcccagac
    25441 cgtaaactgt ttcgccaacg ccaagatcaa gccgctcaaa catataattg cgttgattat
    25501 tcgtgtcctg cacgtagcca ttatttttca cctgactacc ggtaatacgt tcgccgttgc
    25561 gcagaaaatc cagtgaccag aactcacctt tgctgacacg cgcgcttaag ttgccacttt
    25621 taaactcagc ataacgttct gtgttttcga ttgtgacctt cacgtcctgc aaaatattga
    25681 gcggataatg aggaccgtta ttcagcgccc cctgaaaata ctcaatccgc acaccgacaa
    25741 taccttcctg tggggagaaa aagcgcagcg taaataaagg cgtatcaagc tgccaggtac
    25801 gttcacgcac atcacggggg gcagcataga ccaccatttc attatcctgc tgttcaacct
    25861 cgaatacctg aagcgggtga atcaaattga gcccaggttg aatcaaccag tttccatcgc
    25921 taattttcat tgccgttcct tagttctgca cttcttgaga ggtgaattgt tgctggcaac
    25981 ggcgtttacc ctgagccagc tgttccataa cggttttcag attgtgcgtc gtgagtgagt
    26041 agtagcgttt agcgataatc gcgctcagca aataacagat cgccggaaca atcgtgaata
    26101 gcgcaataat gatgctaatc gtggcgctgt tctgcgcttt ttctgccgca tcatatccgc
    26161 cataagccag catccagccg ataagagcgc cgccgaaggc caaccccagt ttgagcacaa
    26221 acagcgtgcc agcaaaactg atcccggtca ggcgtttacc attgcaccac tcgccgtagt
    26281 cgacggtatc gaacatcatt acccactgga taggtgtcac cagttgatgc aacacaccaa
    26341 tcacgaagat gaagacaaac atagtgatgc tggcctgcat gggaacaaag aacatcgcga
    26401 ggctaatcac tgccagcagg gcgttcgtcc accagaagat agtgacttta catttccagt
    26461 cggtcagagg ttttgccagt gcggaaccaa tcaggttacc cacgcaataa gtggtgagaa
    26521 aagcgacaaa cacttccggc gtgcccaaaa tccatgtgac gtaatacatc atcgccccac
    26581 cgcgtacgca caccgccagg atattgaaaa tggttagtaa accgacaatc cgccactggt
    26641 cgttttgcca gatatcacgt aaatcttccc gcatagacgt tgttgtaggt ggtgcttcaa
    26701 cgcgttcttt agtggtgaag aaacaaaatg ccagcatcat taatgccacc acggaaagga
    26761 ccacgatacc gccctggaaa ccgagtggtt tattatcacc gccaattaaa ttaaccagtg
    26821 gcatcatcag aacagtagaa agcatgcctc ccgcggtcgc cagcacaaaa cgccaggatt
    26881 gcagcgagat acgctgagtc gggtcattgg tgattacacc acccaatgcg cagtaaggga
    26941 tattgacgac ggtataaagt aaggtaagtt agggtgtaag taattgctgc atagatcatt
    27001 ttgccgttca tactgagatc tggcgtgcta taggccagta cacagacgat cccgaatggc
    27061 agtgcgccaa acagtaccca cggacgaaat ttaccccagc gagagcgcgt tcgatcggcc
    27121 aacaacccca tgcaaggatc ggaaatcgca tccagtgcac gagcgaccaa aaacatggtt
    27181 ccgacaaatc cggcaggaat gccaaaaata tcggtataaa agaacatcat atataacatt
    27241 acgttatcga aaataatgtg gctggcggcg tctcccatgc cataaccaat tttctcttta
    27301 acggacaaca cttcactctt catctgcttt tccttgatgc tataggggtg ctgagaccga
    27361 taacgcattt tgtaaaccat ccctgtgaga gcgaatattc cgatatctgg ttattaattt
    27421 atgattcttg ttttatgtga tcgtggtagc gttaattccg ctcatatatc attgtaaaat
    27481 atgggtttta tataaattat aatgctttcg tgataatacg ctgcgtttat taggcggaaa
    27541 aaactgatct gggggatgta gaaactcaag gaagtagcta taatgcgccc cgcctccatg
    27601 tagcaatcga ggcgcggaag atcgtcgtct ccggtgaggc ggctggactt caaatccagt
    27661 tggggccgcc agcggtcccg ggcaggttcg actcctgtga tcttccgcca gtttacctct
    27721 tctgaactct cccaatgtca ataaatctct tctaatccct tgtatatcag tatttatttg
    27781 tatcctgagg tcaacctact tcaccccgta tcaagtttct tttgggggta ctttaggggg
    27841 tgttatttcc tattgaaata caagcacccc cataatagga aaaatgtgat acataacaac
    27901 ggcttttaac aatatctttg ttatacaagg ggtgtattgg gggtatcagg ttagttactg
    27961 gcagaaatcc ccctcaggaa catgaattca tcaaaagccg gaggatgtca tggcattaac
    28021 cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg ctgatggcgg
    28081 cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga agtaccgctt
    28141 tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc tagccgatgc
    28201 tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc caggggaggc
    28261 aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg aggctattgc
    28321 tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg atgaaatcat
    28381 tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg ccgaaatcaa
    28441 accaatggag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc tggagaaaat
    28501 gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta ctggccgggc
    28561 agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa aagtacattt
    28621 tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg gttataccgg
    28681 aagtataatc actaagacag ctactcagat cattatgtta accggcgtac gaacacaaga
    28741 actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg agatcccggc
    28801 agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg tcattatgct
    28861 atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg gtagaaatga
    28921 tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac ttggatataa
    28981 gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc atgaacaagg
    29041 ctttaattcc gccttgattg aaatgcagtt agctcatgtg gataaaaatt ccatcagggg
    29101 tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat ggtacgctga
    29161 ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc cctgatgttt
    29221 tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg ttattatatt
    29281 atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt gacattttta
    29341 aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca aaggaaaagt
    29401 tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa tgagattcac
    29461 ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag caatattcat
    29521 tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc agactatatc
    29581 accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag tgcacgataa
    29641 caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg agtttgaaga
    29701 agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca acaaactccg
    29761 acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg catatgcatt
    29821 tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac tggcggcagg
    29881 taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc ccgaaaaccg
    29941 gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac agcgccatca
    30001 ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaagata atcagggggc
    30061 tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag ccttcagtgt
    30121 actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc tcctgcattt
    30181 tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg ccacaaacac
    30241 ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg tgtggcatga
    30301 cttctccggc tctctggata cggcagaaga ttactatgca acatactcac cattatgggg
    30361 acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct ccgtgctgca
    30421 tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg atttcgaaat
    30481 atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca agacactttc
    30541 tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc cctggatatt
    30601 gcccttttcc gcagcaggga tgaaacattc aagaatccag tatcagtaga tattgcatta
    30661 gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt tccttcccca
    30721 ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag cgttgtaaat
    30781 tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt tatcgtcaaa
    30841 cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa tcccctcctc
    30901 tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat aaacagtaaa
    30961 aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg tgcttgtgtt
    31021 ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat tgcatccaac
    31081 ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa atcaatggat
    31141 ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc tcatactggc
    31201 gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa gacgcccaaa
    31261 atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt gtcaataaat
    31321 tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc ccaaaagttt
    31381 atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat attgttacac
    31441 cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg ccagcgacct
    31501 ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt ggtgagattg
    31561 ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg tggacacggc
    31621 cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag gcactgtgac
    31681 gacgccagcg attgtaatca cactcgatat a
//
LOCUS       DAJTNM010000036        30372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTNM010000036 DAJTNM010000000
VERSION     DAJTNM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.036"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..378
                     /gene="tuf"
                     /locus_tag="OO172_001749"
     CDS             <1..378
                     /gene="tuf"
                     /locus_tag="OO172_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5511961.1"
                     /translation="RAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILS
                     KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLS"
     gene            608..991
                     /gene="secE"
                     /locus_tag="OO172_001750"
     CDS             608..991
                     /gene="secE"
                     /locus_tag="OO172_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5511962.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            993..1538
                     /gene="nusG"
                     /locus_tag="OO172_001751"
     CDS             993..1538
                     /gene="nusG"
                     /locus_tag="OO172_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5511963.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1697..2125
                     /gene="rplK"
                     /locus_tag="OO172_001752"
     CDS             1697..2125
                     /gene="rplK"
                     /locus_tag="OO172_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5511964.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2129..2833
                     /gene="rplA"
                     /locus_tag="OO172_001753"
     CDS             2129..2833
                     /gene="rplA"
                     /locus_tag="OO172_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5511965.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3246..3743
                     /gene="rplJ"
                     /locus_tag="OO172_001754"
     CDS             3246..3743
                     /gene="rplJ"
                     /locus_tag="OO172_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5511966.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3810..4175
                     /gene="rplL"
                     /locus_tag="OO172_001755"
     CDS             3810..4175
                     /gene="rplL"
                     /locus_tag="OO172_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5511967.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4495..8523
                     /gene="rpoB"
                     /locus_tag="OO172_001756"
     CDS             4495..8523
                     /gene="rpoB"
                     /locus_tag="OO172_001756"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5511968.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGELVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8600..12823
                     /gene="rpoC"
                     /locus_tag="OO172_001757"
     CDS             8600..12823
                     /gene="rpoC"
                     /locus_tag="OO172_001757"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5511969.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="OO172_001758"
     CDS             complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="OO172_001758"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5511970.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14195..14965)
                     /gene="thiG"
                     /locus_tag="OO172_001759"
     CDS             complement(14195..14965)
                     /gene="thiG"
                     /locus_tag="OO172_001759"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5511971.1"
                     /translation="MLRIVDKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="OO172_001760"
     CDS             complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="OO172_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5511972.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="OO172_001761"
     CDS             complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="OO172_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5511973.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTADINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="OO172_001762"
     CDS             complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="OO172_001762"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5511974.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="OO172_001763"
     CDS             complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="OO172_001763"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5511975.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIHRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18510..18610)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="OO172_001764"
     CDS             complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="OO172_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5511976.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19234..20007
                     /gene="nudC"
                     /locus_tag="OO172_001765"
     CDS             19234..20007
                     /gene="nudC"
                     /locus_tag="OO172_001765"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5511977.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20047..21111
                     /gene="hemE"
                     /locus_tag="OO172_001766"
     CDS             20047..21111
                     /gene="hemE"
                     /locus_tag="OO172_001766"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5511978.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21268..21939
                     /gene="nfi"
                     /locus_tag="OO172_001767"
     CDS             21268..21939
                     /gene="nfi"
                     /locus_tag="OO172_001767"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5511979.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21982..22572
                     /locus_tag="OO172_001768"
     CDS             21982..22572
                     /locus_tag="OO172_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5511980.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22759..23031
                     /gene="hupA"
                     /locus_tag="OO172_001769"
     CDS             22759..23031
                     /gene="hupA"
                     /locus_tag="OO172_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5511981.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23044..23739
                     /locus_tag="OO172_001770"
     CDS             23044..23739
                     /locus_tag="OO172_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5511982.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRASFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="OO172_001771"
     CDS             complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="OO172_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5511983.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24404..25780
                     /gene="zraS"
                     /locus_tag="OO172_001772"
     CDS             24404..25780
                     /gene="zraS"
                     /locus_tag="OO172_001772"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5511984.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25777..27102
                     /gene="zraR"
                     /locus_tag="OO172_001773"
     CDS             25777..27102
                     /gene="zraR"
                     /locus_tag="OO172_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5511985.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27099..28388)
                     /gene="purD"
                     /locus_tag="OO172_001774"
     CDS             complement(27099..28388)
                     /gene="purD"
                     /locus_tag="OO172_001774"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5511986.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28400..29989)
                     /gene="purH"
                     /locus_tag="OO172_001775"
     CDS             complement(28400..29989)
                     /gene="purH"
                     /locus_tag="OO172_001775"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5511987.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7111 a         7914 c         8360 g         6987 t
ORIGIN      
        1 cgtgctggtg agaacgtagg tgttctgctg cgtggtatca aacgtgaaga aatcgaacgt
       61 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      121 tacattctgt ccaaagatga aggcggtcgt catactccgt tcttcaaagg ctaccgtccg
      181 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      241 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc tgatccaccc gatcgcgatg
      301 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgta
      361 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
      421 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      481 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      541 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      601 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      661 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      721 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      781 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      841 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      901 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      961 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
     1021 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
     1081 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
     1141 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
     1201 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
     1261 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
     1321 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1381 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1441 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1501 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1561 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1621 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1681 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1741 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1801 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1861 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1921 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1981 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     2041 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     2101 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     2161 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     2221 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     2281 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     2341 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2401 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2461 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2521 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2581 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2641 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2701 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2761 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2821 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2881 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2941 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     3001 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     3061 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     3121 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     3181 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     3241 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     3301 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3361 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3421 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3481 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3541 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3601 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3661 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3721 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3781 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3841 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3901 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3961 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     4021 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     4081 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     4141 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     4201 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     4261 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     4321 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4381 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4441 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4501 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4561 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4621 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4681 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4741 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4801 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4861 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4921 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4981 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     5041 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     5101 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     5161 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     5221 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     5281 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     5341 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5401 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5461 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5521 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5581 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5641 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5701 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5761 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5821 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5881 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5941 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     6001 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     6061 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     6121 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     6181 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     6241 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     6301 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6361 ctggatgaag gaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6421 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6481 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6541 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6601 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6661 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6721 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6781 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac tggttgaacg tggcgacgtg
     6841 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6901 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6961 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     7021 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     7081 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     7141 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     7201 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     7261 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     7321 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7381 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7441 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7501 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7561 cacgagttcg agaagaaact cgaagcgaaa cgccgaaaaa tcacccaggg cgacgatctg
     7621 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7681 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7741 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7801 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7861 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7921 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7981 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     8041 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     8101 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     8161 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     8221 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     8281 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     8341 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8401 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8461 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8521 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8581 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8641 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8701 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8761 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8821 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8881 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8941 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     9001 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     9061 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     9121 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     9181 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaactaagcg
     9241 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     9301 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9361 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9421 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9481 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9541 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9601 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9661 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9721 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9781 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9841 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9901 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9961 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
    10021 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
    10081 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
    10141 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
    10201 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
    10261 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
    10321 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10381 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10441 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10501 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10561 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10621 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10681 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10741 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10801 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10861 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10921 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10981 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    11041 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    11101 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    11161 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    11221 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    11281 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    11341 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11401 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11461 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11521 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11581 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11641 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11701 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11761 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11821 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11881 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11941 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    12001 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagaaccggc
    12061 aatcttggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    12121 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    12181 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    12241 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    12301 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12361 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12421 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12481 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12541 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12601 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12661 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12721 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12781 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12841 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12901 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12961 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    13021 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    13081 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    13141 gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    13201 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    13261 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    13321 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13381 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13441 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13501 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13561 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13621 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13681 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13741 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13801 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13861 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13921 ccgcagtacg tgcagttgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13981 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    14041 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    14101 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    14161 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    14221 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    14281 gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    14341 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgcggcat
    14401 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14461 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14521 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14581 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14641 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14701 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14761 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14821 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14881 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14941 acgttttgtc cacaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    15001 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    15061 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    15121 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    15181 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    15241 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    15301 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15361 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15421 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15481 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15541 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15601 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15661 cgggctgttg atatctgcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15721 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15781 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15841 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15901 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15961 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    16021 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    16081 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    16141 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    16201 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    16261 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    16321 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16381 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16441 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16501 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16561 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16621 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16681 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16741 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16801 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16861 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16921 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16981 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    17041 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggtg
    17101 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    17161 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    17221 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    17281 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    17341 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17401 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17461 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17521 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17581 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17641 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17701 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17761 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17821 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17881 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17941 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    18001 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    18061 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    18121 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    18181 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    18241 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    18301 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18361 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18421 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18481 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18541 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18601 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18661 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18721 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18781 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18841 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18901 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18961 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    19021 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    19081 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    19141 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    19201 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    19261 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    19321 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19381 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19441 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19501 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19561 accgaatggg cgatgctgtg cagccattgc cgtgagcgtt actacccgca aatcgccccc
    19621 tgcattattg ttgccatccg tcgcgatgat tcgatcctcc tcgcccagca tacccgccat
    19681 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19741 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19801 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19861 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19921 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19981 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    20041 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    20101 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    20161 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    20221 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    20281 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    20341 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20401 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20461 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20521 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20581 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20641 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20701 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20761 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20821 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20881 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20941 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    21001 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    21061 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    21121 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    21181 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    21241 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    21301 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21361 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21421 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21481 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21541 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21601 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21661 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21721 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21781 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21841 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21901 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21961 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    22021 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    22081 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    22141 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    22201 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    22261 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    22321 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22381 caggctggtc gcgaaatgag cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22441 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22501 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22561 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22621 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22681 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22741 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22801 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22861 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22921 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22981 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    23041 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    23101 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    23161 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    23221 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    23281 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    23341 ccgccggtga acattcgcgc gagttttgac gatcgcggcg atctgagctt tatgcaacgt
    23401 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23461 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23521 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23581 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23641 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23701 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23761 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23821 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23881 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23941 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    24001 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    24061 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    24121 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    24181 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    24241 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    24301 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24361 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24421 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24481 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24541 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24601 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24661 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24721 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24781 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24841 ttatcgccag tttcaaccga tgtttgctgc tggaatgcac cggatgcgcc atatgcagca
    24901 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24961 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    25021 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    25081 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    25141 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    25201 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    25261 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    25321 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25381 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25441 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25501 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25561 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25621 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25681 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25741 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25801 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25861 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25921 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25981 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    26041 gtcgagacgg cggtagaggc actgaaaact ggggcgctgg attatctcat caagccgctg
    26101 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    26161 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    26221 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    26281 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    26341 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26401 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26461 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26521 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26581 cagactatct cggttgatgt ccggctgatt gcggcgactc atcgcgatct tgccgcagag
    26641 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26701 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26761 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26821 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26881 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26941 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    27001 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    27061 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    27121 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    27181 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    27241 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    27301 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27361 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27421 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27481 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27541 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27601 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27661 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27721 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27781 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27841 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27901 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27961 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    28021 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    28081 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    28141 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    28201 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    28261 agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc
    28321 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28381 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28441 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28501 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28561 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28621 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28681 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28741 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28801 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28861 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28921 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28981 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    29041 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    29101 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    29161 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    29221 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    29281 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    29341 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29401 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29461 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29521 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29581 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29641 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29701 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29761 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29821 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29881 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29941 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatccct
    30001 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    30061 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    30121 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    30181 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    30241 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    30301 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30361 ttcggttgaa ga
//
LOCUS       DAJTNM010000037        29684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTNM010000037 DAJTNM010000000
VERSION     DAJTNM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.037"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            217..1665
                     /gene="uacT"
                     /locus_tag="OO172_001776"
     CDS             217..1665
                     /gene="uacT"
                     /locus_tag="OO172_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5511988.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1667..1792
                     /locus_tag="OO172_001777"
     CDS             1667..1792
                     /locus_tag="OO172_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511989.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1789..1860)
                     /locus_tag="OO172_001778"
     CDS             complement(1789..1860)
                     /locus_tag="OO172_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5511990.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            1915..2463
                     /gene="idi"
                     /locus_tag="OO172_001779"
     CDS             1915..2463
                     /gene="idi"
                     /locus_tag="OO172_001779"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5511991.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            complement(2506..4023)
                     /gene="lysS"
                     /locus_tag="OO172_001780"
     CDS             complement(2506..4023)
                     /gene="lysS"
                     /locus_tag="OO172_001780"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5511992.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4033..5131)
                     /gene="prfB"
                     /locus_tag="OO172_001781"
     CDS             complement(join(4033..5055,5057..5131))
                     /gene="prfB"
                     /locus_tag="OO172_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5511993.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5222..6955)
                     /gene="recJ"
                     /locus_tag="OO172_001782"
     CDS             complement(5222..6955)
                     /gene="recJ"
                     /locus_tag="OO172_001782"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5511994.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(6961..7671)
                     /gene="dsbC"
                     /locus_tag="OO172_001783"
     CDS             complement(6961..7671)
                     /gene="dsbC"
                     /locus_tag="OO172_001783"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5511995.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(7696..8592)
                     /gene="xerD"
                     /locus_tag="OO172_001784"
     CDS             complement(7696..8592)
                     /gene="xerD"
                     /locus_tag="OO172_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5511996.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8704..9225
                     /gene="fldB"
                     /locus_tag="OO172_001785"
     CDS             8704..9225
                     /gene="fldB"
                     /locus_tag="OO172_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5511997.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9265..9672)
                     /locus_tag="OO172_001786"
     CDS             complement(9265..9672)
                     /locus_tag="OO172_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5511998.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9653..9919)
                     /gene="sdhE"
                     /locus_tag="OO172_001787"
     CDS             complement(9653..9919)
                     /gene="sdhE"
                     /locus_tag="OO172_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5511999.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            10162..11142
                     /gene="ygfZ"
                     /locus_tag="OO172_001788"
     CDS             10162..11142
                     /gene="ygfZ"
                     /locus_tag="OO172_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5512000.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11338..11997)
                     /locus_tag="OO172_001789"
     CDS             complement(11338..11997)
                     /locus_tag="OO172_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5512001.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12161..12472)
                     /locus_tag="OO172_001790"
     CDS             complement(12161..12472)
                     /locus_tag="OO172_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR5512002.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            12517..13949
                     /gene="bglA"
                     /locus_tag="OO172_001791"
                     /pseudo
     CDS             12517..13949
                     /gene="bglA"
                     /locus_tag="OO172_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
     gene            complement(14006..14749)
                     /locus_tag="OO172_001792"
     CDS             complement(14006..14749)
                     /locus_tag="OO172_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5512003.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15016..17889)
                     /gene="gcvP"
                     /locus_tag="OO172_001793"
     CDS             complement(15016..17889)
                     /gene="gcvP"
                     /locus_tag="OO172_001793"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5512004.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18008..18397)
                     /gene="gcvH"
                     /locus_tag="OO172_001794"
     CDS             complement(18008..18397)
                     /gene="gcvH"
                     /locus_tag="OO172_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5512005.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18421..19515)
                     /gene="gcvT"
                     /locus_tag="OO172_001795"
     CDS             complement(18421..19515)
                     /gene="gcvT"
                     /locus_tag="OO172_001795"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5512006.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLIVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            19806..19889
                     /locus_tag="OO172_001796"
     CDS             19806..19889
                     /locus_tag="OO172_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512007.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            complement(19963..21165)
                     /gene="ubiI"
                     /locus_tag="OO172_001797"
                     /gene_synonym="visC"
     CDS             complement(19963..21165)
                     /gene="ubiI"
                     /locus_tag="OO172_001797"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5512008.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(21188..22366)
                     /gene="ubiH"
                     /locus_tag="OO172_001798"
                     /gene_synonym="visB"
     CDS             complement(21188..22366)
                     /gene="ubiH"
                     /locus_tag="OO172_001798"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5512009.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22363..23688)
                     /gene="pepP"
                     /locus_tag="OO172_001799"
     CDS             complement(22363..23688)
                     /gene="pepP"
                     /locus_tag="OO172_001799"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5512010.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23714..24292)
                     /gene="ygfB"
                     /locus_tag="OO172_001800"
     CDS             complement(23714..24292)
                     /gene="ygfB"
                     /locus_tag="OO172_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5512011.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24460..24789
                     /gene="zapA"
                     /locus_tag="OO172_001801"
     CDS             24460..24789
                     /gene="zapA"
                     /locus_tag="OO172_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5512012.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24831..25014
                     /gene="ssrS"
                     /locus_tag="OO172_001802"
     ncRNA           24831..25014
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO172_001802"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25035..25637
                     /locus_tag="OO172_001803"
     CDS             25035..25637
                     /locus_tag="OO172_001803"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5512013.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25734..25793)
                     /locus_tag="OO172_001804"
     CDS             complement(25734..25793)
                     /locus_tag="OO172_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5512014.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26126..26185)
                     /gene="ibsC"
                     /locus_tag="OO172_001805"
     CDS             complement(26126..26185)
                     /gene="ibsC"
                     /locus_tag="OO172_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5512015.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26414..27646)
                     /gene="serA"
                     /locus_tag="OO172_001806"
     CDS             complement(26414..27646)
                     /gene="serA"
                     /locus_tag="OO172_001806"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5512016.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(27902..28561)
                     /gene="rpiA"
                     /locus_tag="OO172_001807"
     CDS             complement(27902..28561)
                     /gene="rpiA"
                     /locus_tag="OO172_001807"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5512017.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            28944..>29684
                     /gene="argP"
                     /locus_tag="OO172_001808"
     CDS             28944..>29684
                     /gene="argP"
                     /locus_tag="OO172_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5512018.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEK"
BASE COUNT         7045 a         8002 c         7452 g         7185 t
ORIGIN      
        1 ttaatgaaat atcaatatga gaaaattaca gataattcca ttgtcaggtt ctcttgtcat
       61 tttcgtggtt taagcacgat tatgtgattg cctccgcaat tcctgtctct aactcccctt
      121 cctcgcaaaa actggcactc cacgagcatg tgtttagaca gtttcattaa cgtaaacggt
      181 tgctttttat tctggcgggc gaaaggagaa acactgatga gcgccataga ttcccaactt
      241 ccctcatctt ctgggcaaga ccgcccaact gatgaggttg accgcatatt atcaccagga
      301 aagctgatca tactcggtct gcaacacgtc cttgtcatgt acgcaggtgc agtcgctgtt
      361 cctcttatga ttggtgaccg actgggcctc tcaaaagatg ctattgcgat gctcattagc
      421 tcggatctct tttgctgcgg gatcgtcaca ttattgcaat gtatcggtat cggccgcttt
      481 atggggatcc gcctgccggt gattatgtcg gtgacctttg ctgctgtaac accaatgata
      541 gccattggga tgaacccgga tatcggcctg ctggggatat ttggtgccac tatcgccgcg
      601 ggttttatca ccacattatt agcgccactt attggtcgct tgatgccttt attcccgcca
      661 ctggttaccg gtgtggttat tacttctatt gggcttagca tcattcaggt gggtattgac
      721 tgggccgctg gaggtaaagg gaatccgcaa tatggtaatc ccgtttattt aggtatctcc
      781 tttgccgtct taatttttat cttgctcatt actcgctatg cgaaaggatt tatgtccaac
      841 gtcgccgtat tactggggat tgtatttggc tttttacttt cgtggatgat gaatgaagtc
      901 aatttatccg ggctacatga tgcctcgtgg tttgcgattg tcaccccgat gtcgtttggt
      961 atgccgattt tcgatcccgt ttccattctg accatgactg ccgtgttaat catcgtgttt
     1021 atcgagtcga tggggatgtt cctggcactg ggtgaaatag tcggtcgtaa actctcttca
     1081 cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga caatgatagg cggcacgttt
     1141 aacagcttcc cccacacgtc attttctcaa aacgttggcc tggttagcgt gatgcgcgtt
     1201 catagccgct gggtgtgtat ttcttcggga attatattaa tcctgtttgg catggtgcca
     1261 aaaatggcgg tgctggtagc ctccattccg caatttgtgc tgggcggcgc tggtctagtg
     1321 atgttcggca tggtactggc gacagggatt cgaattctgt cgcgctgtaa ctacaccacc
     1381 aaccgttaca acctctatat tgtggcgatc agtctcggcg ttggcatgac tccgacgctc
     1441 tctcacgatt tcttttctaa gttaccggcc gtactgcaac cgctgctaca tagcggcatt
     1501 atgctcgcaa cccttagcgc cgttgtgctg aacgtcttct ttaatggcta tcagcatcat
     1561 gctgacctgg tgaaggaatc cgtctctgat aaagatttaa aagtcaggac agtacgtatg
     1621 tggcttctga tgcgcaagct gaagaaaaat gagcatggag aataatatga attttttaat
     1681 gcgcgctata ttcagtctgt tgttgctttt tactctctct attcctgtca tttctgactg
     1741 tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg ctactttttt aaatggtttt
     1801 tacctgtcgg catccgctca aaacgggcgg ttgtcgataa acgctctctt ggttaatcat
     1861 ttcactcttc aattatctat aatgatgagt gatcagaatt acatgtgaga aattatgcaa
     1921 acggaacacg tcattttatt gaatgcacag ggagttccca cgggtacgct ggaaaagtat
     1981 gccgcacaca cggcagacac cctcttacat ctcgcgttct ccagttggct gtttaatgcc
     2041 aaggggcaat tattagttac ccgccgcgca ctgagcaaaa aagcgtggcc tggcgtgtgg
     2101 actaactcgg tttgtgggca cccacaactg ggagaaagca gcgaagacgc ggtgatccgc
     2161 cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg aatctatcta tcctgacttt
     2221 cgctaccgcg ccaccgatcc gagtggcatt gtggaaaatg aagtgtgtcc ggtatttgcc
     2281 gcacgcacca ccagtgcgtt acagatcaac gatgatgaag tgatggatta tcaatggtgt
     2341 gatttagctg atgtattacg cggtattgat gccacgccgt gggcgttcag tccgtggatg
     2401 gtgatgcagg cgacaaatcg cgaagccaga atacgattat ctgcatttac ccagcttaaa
     2461 taaaaaaccc cgacatttgc cggggttgtg agcataacgt aatgcttatt tcaccggacg
     2521 catcgccggg aacagaataa cgtcgcggat ggtatggctg ttggtgaaca gcattaccat
     2581 acggtcgatg ccaatcccca gacctgctgt cggcggtaag ccatgttcca gtgcggtaac
     2641 gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg tctttcgcgg caacctgatc
     2701 caggaagcgt tgcgcctgat cttccgcgtc attcagctcg ctaaagccgt taccgatttc
     2761 acgaccaccg atgaagaact caaagcggtc tgtgatttcc gggttaatgt cgttacgacg
     2821 cgccagcgga gaaacttctg ccggatattc agtaatgaag gtcggctgaa tcagatgtgc
     2881 ttctgccact tcttcgaaga tctcggtaac gatacggccc agaccccagc tcttctcaac
     2941 gtggataccg atagattcag caatcgcttt cgcagagtcg aagttatcca gatccgccat
     3001 gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg gtcagttttt cgaacggttt
     3061 accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc ttaccgagaa tatcctgtgc
     3121 cagagtacgg aacagcgatt cggtcagctc gatcagatct ttgtaatctg cgtaagccat
     3181 gtagagttcc atcatggtga actctgggtt atgacgtacg gaaatacctt cgttacggaa
     3241 gttacggttg atttcgaata cacgctcgaa gccaccgacc accagacgct tgaggtacag
     3301 ttccggcgcg atacgcaggt acatgtcgag atccagcgcg ttatggtggg tgataaacgg
     3361 acgcgcagcg gcaccgcctg ggatcacctg catcatcggc gtttcaactt ccataaagcc
     3421 acggttcacc atgaactggc gaataccaga gaggatctgc gagcgtactt taaaggtgtt
     3481 acgggattca tcgttagaga tgagatccag ataacgctga cgatagcgcg cttcctgatc
     3541 ctgcaagccg tggaatttat ccggcagcgg acgcagtgct ttggtcagca gacgcagctc
     3601 ggtacagtgg atagacagtt cgccggtttt ggttttgaac agtttacctt tcgcgccgag
     3661 gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa acgccttccg gcagatcgtc
     3721 acgggcaacg tacagctgaa tgcggccacc cacatcctgc agggtaacga aagacgcttt
     3781 acccataata cggcgggtca tcatgcggcc agcaacggcg acttcgatgt tcagcgcttc
     3841 cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat tggtcagagg tatgatcgcg
     3901 acggaaatcg ttcgggaagg caattccctg ctcacgcagg ttcgccagct tctcacgacg
     3961 cgttttcagt tcattgttaa gatcgactac cgcgtcagcg ccctgtgcgt gttgttcaga
     4021 catgttggtt cctcataacc ctgctttcaa acttgcttcg ataaattgat ccaggctgcc
     4081 gtccagcacg gcctgcgtgt tgcgagtttc taccccggtg cgcagatctt taatgcggga
     4141 gtcatcaagg acataagaac gaatctggct gccccagccg atgtcagatt tgttatcttc
     4201 catcgcctgt ttctcggcat ttttcttctg catctccagt tcataaagct tcgctttcat
     4261 ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg tcgttctggc actgggtcac
     4321 gatcccggtc gggatgtggg taatacgcac cgcagattcg gtacggttaa cgtgctgacc
     4381 gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc gccgggttga tttcgatatc
     4441 aatatcatca tcaacttccg gataaacaaa cgcagagctg aacgacgtgt ggcgacgacc
     4501 gccggagtca aacgggcttt tacgcaccag gcggtgaacg cccgtttctg tacgcagcca
     4561 gccgtaagcg taatcgccgg agattttgat cgtcacggat ttaatacccg ccacttcacc
     4621 ttccgactct tcgatgattt cagttttgaa accacgcgat tctgcccagc gcagatacat
     4681 acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg ccagaccccg cctgaatatc
     4741 gaggtaacag tcggcgctgt catattcgcc agagaacata cggcggaact caagctgcgc
     4801 cagtttttct tccagggcgt cgagttcagc aacggcttcg ttaaaggttt cttcgtcgtc
     4861 agcttctaca gccagttcca gcagaccaga aacatcttcc agcccctgtt tcatttggtc
     4921 gagggtgtcg acaacggctt cgagggagga acgctcttta cccagcgcct gtgcgcgttc
     4981 gggttcgttc cagacatccg gctgttccag ctcggcgttt acttcttcca gacgctcttt
     5041 cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga gcgttccgtg aggtcctgaa
     5101 tgcgattatt taccggatta atttcaaaca tggtctgatt tcttttattg agctagtcaa
     5161 aatgcggtga taagagcggg attgtaccca atccacgctc ttttttatag agaagatgac
     5221 gctaaattgg ccagatattg tcgatgataa tttgcaggct gcggttgccg cgaaactcgt
     5281 tgatatcgag cttataagcc agttgcactt cgcggacgcc gttatccggc cagagagcgg
     5341 tatcgacatt aaaagcaata ccatccagca gtggaccgcc gccgaccggt tcgaccatca
     5401 ccttcaaatg acgttcgccc accagccgct gttgcagcag acggaaatga ccgtcaaaca
     5461 gcggctccgg gaacatctgc ccccacgggc cagcatcgcg cagcagctgc gccacttcca
     5521 tggtcatttc ggccgggctt aacggaccgt ctgacaccac ttcgccttgc aatagcgaag
     5581 gggccagcca ctcagtaacc agttcgccaa accgttgttg aaagagttcg aatttatcct
     5641 cttccagcga caaacccgcc gccatcgcat gaccgccaaa cttgagcatc atgccggggt
     5701 agagtgtgtc taatcgctcc agcgcatcac gcatatgcag cccctgaatg gagcgaccgg
     5761 aacctttcag cgtaccgtca cctgctggcg caaaggcgat aaccggacgg tgaaaacgct
     5821 ctttgatacg cgaagccaga ataccgacaa cgccctgatg ccattcgggg tgatacattg
     5881 ccagcccgcc gggtagcgtg tcacggctgc gctccagttt ctcgcacagg gtcagggctt
     5941 caacctgcat tccttgttcg atctcttttc gcgtctggtt tagcgcatcg agttcatttg
     6001 ccagcacgcg cgcttcgccg atgttgtcgc acagcaacag cgccacacca acggacatat
     6061 catccagtcg cccggcagca ttgagacgcg gtcccagcgc aaaacctaaa tcgctggcgg
     6121 ctagtttttg tgcatcgcga tttgccactt ccagcagcgc tttaatccct ggacggcact
     6181 ttccggcacg gatgcgactc atcccctgcc aggtcagaat gcgattatta gcgtccagcg
     6241 gcacgacgtc cgctactgtc cccagcgcta ccagatccag cagttctgcc aggttaggaa
     6301 ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa aaaggtgcgc agcgccagca
     6361 tcagataaaa cgccacaccc acgcctgcca gtgatttcga cgggaaatta cagtcgcgca
     6421 agttagggtt aatgatcgct tccgctgcgg gtaatgtgtc gcccggcaaa tggtgatcgg
     6481 taacaataac cgggatgccc aacgagcgag cgtgctcaac tcccgcatgg gaggaaatac
     6541 cgttatccac cgtgacaatt aactgcgcgc cacgggcatg ggcctgatcg accacttccg
     6601 ggcttaagcc gtaaccgtct tcgaaacggt ttggtaccag gtagtcgata ttgctgcaac
     6661 caagcgagcg catcgccagc acgcttagag ccgtgctggt cgcgccgtcg gcgtcgaaat
     6721 caccgaccac aataatccgc gttccttcgc gaaaagcgtt gtaaaggatc tcaacggcct
     6781 tttcgacacc gcttagttgc tgccagggca acataccttt aacactgcgt tccagttctt
     6841 gcgcactgcg tactccccgg ctggcgtata aacggcgcag caagggaggc aattcagcgg
     6901 gcaagtctgc cgtttcatcg acttcacggc gacgaagttg tatctgttgt ttcacgcgaa
     6961 ttatttaccg ctggtcattt tttggtgttc gtcgaggaat tctttcatgt ctttcggcgg
     7021 ctggtaaccc ggaacaagtg tgccattgct cagcacaact gccggagtac cgctaacgcc
     7081 aagctggacg ccaagtgcgt aatggtcggc aatatccacg tcgcagctgg ctggtgcgac
     7141 gcttttacct gccatcacat catcaaacgc tttgttttta tctttcgcac accagatagc
     7201 tttcatttct ttctctgcat cgctgtccag cccctggcgc gggaaagcaa gataacgcac
     7261 ggtgatcccc agcgcgttat agtctgccat ttgctcatgc agtttgtggc agtaaccaca
     7321 ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc ggcgctttat aaacgatcat
     7381 ctctttttca agcgcattca actgctttaa cagcatctta ttggtgacat tgaccggagc
     7441 cgtgccacta acgtcataca ttggcccctg aatgatatgt ttaccatcat cggtgatgta
     7501 caacacaccg ctgttagtca gaactgtctt catgccagct acgggcgcgg gctgaatatc
     7561 gctgcttttg atgcccattt tggctaacgt ttgttgaatt gccgcgtcat cagcctgagc
     7621 aaagcctgaa aacgccgcta acaaagtaaa caacataaaa cctttcttca taaatcttcc
     7681 cgttcttttc agacatcacg cccgcgggtg atgctgttga tgaagttgcc gcagacgctc
     7741 ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg ctgtggccca gtagcatctg
     7801 caccacgcgt aaatccgcac catgatttaa taaatgagtg gcaaaagcgt gacgcaacac
     7861 atgcggtgac agcttttcgc tgtcgatacc cgccagcacg gcataatgtt taatgcggtg
     7921 ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg ggaaacaaca cgtcaattga
     7981 cacaccattc agcagccacg gacgcccatg ttccagatag gtttccagcc agtaaaccgc
     8041 ctcttcccct aacggcacca ggcgctcttt gttgccttta ccaatgaccc gcactacgcc
     8101 ctgacgcagg ctgatatcac tcattgtcag cccgaccagt tcagagacac gcagcccggt
     8161 agcatacaac acttcaagca tggctttatc gcgtagctcc agtggctgat caattaatgg
     8221 cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct tttggcaaac gctggggcaa
     8281 tttcggtgaa gcgagatgcg cactgggatc gtcttcacga aacttttcgc gataaagata
     8341 ctggaacaat cggcgcactg cactcagcaa acgcgctgag ctggtggctt tatacccgcc
     8401 ctccagccgt tctgccagta atgcctgcaa atcgtcactt tgcgccgtcg ccagcgtcaa
     8461 cccgcggtga tgcaaccact ccaccatcat tgacagatcg cgacggtaag cgttcaacgt
     8521 attttcagcc agattttttt ccagccacag agcatcaaga aactgctcga tgcgtgccag
     8581 atcctgtttc acttgcgccc cttatggtca ctcatttgat ccattatgcc ttattgtgcc
     8641 gtgactaaag cgattctgat acactagctg caaaagccac agcagaatcg agaagcttac
     8701 gttatgaata tgggtctttt ttacggttcc agcacctgtt acaccgaaat ggcggcagaa
     8761 aaaatccgcg atattatcgg cccagaactg gtgaccttac ataacctcaa ggacgactcc
     8821 ccgaaattaa tggagcagta cgatgtgctc attctgggta tcccgacctg ggattttggt
     8881 gaaatccaag aagactggga agccgtctgg gatcagctcg acgacctgaa ccttgaaggt
     8941 aaaattattg cgctgtatgg gcttggcgat caactgggat acggcgagtg gttcctcgat
     9001 gcgctcggta tgctgcatga caaactctcg accaaaggcg tgaagttcgt cggctactgg
     9061 ccaacggaag gatatgaatt taccagcccg aaaccggtga ttgctgacgg gcaactgttc
     9121 gtgggtctgg cgctggatga aactaaccag tatgacctta gcgacgagcg tattcagagc
     9181 tggtgcgagc aaatcctcaa cgaaatggca gagcattacg cctgatctca ctgacggctt
     9241 agcgcatatg ctttgccgtc atctttatct ttgcgtctct tgttgcaaca aaatccgccg
     9301 taaatcccgc cattcggctt cgtccatgct gtcggctgcc agccataaat gttgccgttt
     9361 accgccatca gaacgtaaac gcagcatcat gccgctctta atcatccacg gtgctttgac
     9421 gatgctccac tcctgccctt gccaacgcaa acgcccgtcc atcaacaagc gaatttcccc
     9481 ctggcgagca ttaatacacc gctggctgcg aacgcaatca aataccacca gcgaaagtaa
     9541 caccatccat aacggggtgt aactgagcgg ccagggcatg agtaaaataa cagcggcaac
     9601 cagcccatga atcagcaagg aaagccactg tgcgcgccag gagacgcgca aatcagattg
     9661 ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg gaccattatt tccagttctg
     9721 catcggccgg tttaccgtga ttcatcagcc agttaaacag gtccggatcg tcacattcca
     9781 gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct gtcgtactca tgttcgaaaa
     9841 acggcatgat tgaaatatcg agttcgcgca taccacggcg gcatgcccaa tgaatgcggg
     9901 ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa tccagtaccc ggctattgta
     9961 acgtgttttt cgacttcttt tacgggaata tcagtaaaca ccatcgcgat cgcgaaatta
    10021 attcacaaca attcaagggc ttcatttttt tggcagtcgc ctcgcagaag gcacagatcg
    10081 cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc attagtcatt aatacgccgt
    10141 taagcaactc aggactctat tatggctttt acaccttttc ctccacgtca gcctacggct
    10201 tctgcccgtt taccgctgac gctgatgacg cttgatgact gggcgcttgc caccattact
    10261 ggcgcggaca gcgaaaaata tatgcagggt caggtgacag cagatgtcag ccagatggca
    10321 gaagatcagc acctgctcgc cgcccattgc gacgccaaag gtaaaatgtg gagcaattta
    10381 cgtctgttcc gcgacggcga tggctttgca tggattgaac ggcgcagcgt gcgtgaaccg
    10441 cagctgactg aactgaaaaa atatgcggta ttctctaaag tgaccatcgc gccagacgac
    10501 gagcgtgtgc tgcttggtgt tgccggtttt caggcgcgcg ccgcgctggc aaatctcttt
    10561 agcgaactgc cttcgaaaga aaaacaggta gtcaaagaag gcgcgaccac tttgctatgg
    10621 tttgaacacc cggcagaacg tttcctgatc gtaaccgatg aagctactgc taatatgctg
    10681 accgataaac tgcgcggtga agcggaactg aacaatagcc aacagtggct tgcattaaac
    10741 attgaagctg gtttcccggt gattgatgcc gccaacagcg ggcagtttat cccacaggcg
    10801 accaatctcc aggcgctggg cggtatcagc tttaagaaag gctgttatac cggacaagag
    10861 atggtggcgc gagcaaaatt ccgtggtgcc aacaaacgcg cgctctggtt gctggcaggt
    10921 agtgccagcc gactgccgga agctggtgaa gacttagagc tgaaaatggg cgagaactgg
    10981 cgtcgtaccg gtacggtgct ggctgcggta aaactggaag atggtcaggt cgtggtacag
    11041 gtcgtcatga ataacgatat ggaaccggac agcatcttcc gcgtacgcga cgatgcgaat
    11101 acactgcata tcgagccgct gccgtattcg ctcgaagagt aaatctctgt atcgcatcag
    11161 gcatttatcg cctgatgcga cgctgacgcg tcttatcatg tctgggattt gatgccttta
    11221 tcacaacagg gacgaggccc gtagatcgga taaggcgttc acgccgcatc cggcggttgc
    11281 gctccgatgc ctgatgcgac gctgacgcgt cttatcaggc ctacaaaggc atacccatta
    11341 cgcctgccca atatacaaat agatcgccag aaagtggcac acactaccgc cgagcacgaa
    11401 gccgtgccag atggcatggt tgtatggaat gcgtttgcag acgtagaaaa tcaccccgag
    11461 cgaataaacc acgccgccta ccgccagtaa ggtaacgctg cccgccgcga gcttaactgc
    11521 catttcataa attaccacca gcgacagcca gcccatcgcc agataagtca ccagagataa
    11581 aattttgaat cggtgcgcga tggtcagttt gaacagaatc cccagcaatg ctaggctcca
    11641 gataacaatc atcaacccgc gcgctaacgg agaatccagc cccaccagca aaaacggcgt
    11701 gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca aatttcttca gccacatttt
    11761 tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg aggaacagca ggatcatact
    11821 gccgccatag aggctgtaac tggttatcgc tgtggcgctg gcattgagat ccaccgcctg
    11881 aaccagtagc aacaccagcc caacgatacc aaacaccagc ccaatgccgt gactgacgct
    11941 gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg aggggcttct gaaccataac
    12001 ttactccaga gaaacgtaca cgcacatgta tacctctcca gcgtaactga gaatagttcc
    12061 agtgaacacc tgttagctaa aataaatttt gatttgagac taatctccta aaaatcatga
    12121 aattaaatgc gaaatttcaa ctaacaggcg tcagttcaat ttaaagacat ttaaattcaa
    12181 tcatataaaa ttgtgtctga ccgggataga tgtcagcaat gacctttttc agttcaggca
    12241 gggtcatatt ttcctgctct gcatgttttt ccgtcagtgt atccagcgtt acggttgagg
    12301 ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc aaaacgtccg acacgaagca
    12361 catcacccgt tttgaagtgc gattcagact cgtcgcggat ggtgatggtt ttacgcccag
    12421 ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc gtttggctgc atggtactat
    12481 ttcctgtaag aattgactga tctggagcct atgatagtga aaaaactcac cttaccgaaa
    12541 gatttcttat ggggtggcgc cgttgccgct catcaggtcg aaggcggctg gaacaaaggc
    12601 ggcaaaggac cgagcatttg tgacgttctg accggtggcg cacacggcgt gccgcgcgaa
    12661 atcaccaaag aagtcttgcc aggaaaatac tatccaaacc atgaagccgt tgatttttat
    12721 ggtcactaca aggaagacat caagctattt gccgaaatgg gcttcaaatg ttttcggact
    12781 tccatcgcct ggacgcgtat ttttccaaaa ggtgatgaag ctcagccaaa cgaagaaggg
    12841 ctgaagtttt acgactctct gttcgatgaa ctgctgaaat acaacatcga accggtgata
    12901 accctctccc actttgaaat gccgctgcat ctggtgcagc aatacggtag ctggaccaac
    12961 cgtaaattcg ttgatttctt tgtccgtttc gcggaagtgg tattcgaacg ctacaagcat
    13021 aaagtcaaat actggatgac cttcaacgaa ataaacaacc agcgtaactg gcgtgcgccg
    13081 ctgtttggtt actgctgctc cggcgtggtg tataccgagc atgacaaccc ggaagagacg
    13141 atgtaccagg tgctgcatca ccagtttgtc gccagcgccc tggcagtgaa agccgcgcgt
    13201 cgcattaacc cggagatgaa agtcggctgt atgctggcga tggtgccgct ctatccctac
    13261 tcatgtaacc cggacgatgt gatgttcgct caggagtcga tgcgcgaacg ctacgtcttt
    13321 accgatgtgc agctatgcgg ctattacccg tcctatgtat tgaacgagtg ggaacgtcgc
    13381 ggatttaata tcaaaatgga agacggcgat ctcgatgtac tgcgcgaagg cacctgcgat
    13441 tatcttggtt tcagctatta catgaccaac gcagtgaagg ccgaaggcgg caccggcgat
    13501 gcgatctctg gttttgaagg cagcgtaccg aacccgtatg ttaaagcgtc tgactggggc
    13561 tgcagattga tccagtaggt ctgcgctatg ccctttgcga actgtatgag cgttatcaga
    13621 ggccgctgtt tattgtcgaa aacggttttg gcgcttacga caaagtggaa gaagatggca
    13681 gcatcaacga cgactaccgc attgactacc tgcgcgccca tattgaagag atgaaaaaag
    13741 cagtgactta cgatggtgtg gatctgatgg gctacacacc gtggggctgc atcgactgcg
    13801 tgtcattcac taccgggcag tacagcaaac gctacggctt tatctatgtg aataaacatg
    13861 acgacggtac tggcgatatg tcgcgttcac gtaagaagag ctttaactgg tacaaagagg
    13921 tgattgccag caacggcgag aagctttaag tcgatgaagt accggatgca atacttgttg
    13981 catccggtca gacaacttcc ctgttttatt tcccgcccgc caaatcgata aaacttcccg
    14041 tgacgtaaga ggctttatca cttagtagcc agacaatggc ctgcgcgacc tcttctgcct
    14101 gtccaccacg ctgcatgggg atgttcgact taacgcgatc gacgcgtcca ggctcgccgc
    14161 cgctggcgtg catttcggta taaataaacc ctggccgcac gcagttaacg cggatcccct
    14221 gcgcggcgac ttccagcgat cgtccggtgg ttaacgtatc aatcgcccct ttcgatgccg
    14281 cgtaatcaac atattcccct ggcgaaccca accgtgaggc caccgaagag acattgacga
    14341 tagcgccgcc actgccacca tttttaagcg ccatgcgttt taccgcctcg cggcagcaga
    14401 gaaaatatcc cgtcacgttg gtggaaagta ctcggttgat tcgctctgcg gtaaggtttt
    14461 caacggtgca ctgggtaaac aagatcccgg cgttattgac cagcgctgct agcggttcat
    14521 cgtgctgatc gattgctgta aacatcgcaa cgacctggtt ttcgtcgctg atatccgcct
    14581 ggagcacgaa tgctttgcca ccggcttgca ttattaagtt catcacttcc tgcgccgcgt
    14641 ggaggttttg ctgataatta accgccaccg tatacccttc ttgcgccaac agtaatgcag
    14701 ttgcccgccc gatgccgcga ctgccaccgg tcacaagtgc tatagccatt tgtttttccg
    14761 agaaaaatta attcgttgac gtatctttga gattgctaac aaactggctc aatcttcaat
    14821 gttggcacgc ttttccccct cgccctttca gggagatggt cggggtgagg gtaaatattc
    14881 gcgccagtgc cggcctgttc ccctcaccct aaccctctcc ccaaaggggc gaggggactg
    14941 tctgagcact tttgtacttt gtcatctgac taaaaaggcg ccgaagcgcc tttagaaaat
    15001 actcgaatca gtgaattact ggtattcgct aatcggtacg caggagcaga acaggttacg
    15061 gtcgccgtaa acatcatcca gacgtttcac cgtcggccag tatttgtctg ccacgcctgc
    15121 cgggaatacc gcaacttcac ggctgtacgg atgcgcccac tcggcgacca gttcgctctg
    15181 aatgtgcggc gcgttcacca gcgggttatc ttccagcggc cagacaccgg ctttcacctg
    15241 gtcaatttct gcgcggatag ccagcatcgc gtcgataaag cgatccagtt ccactttgct
    15301 ttcagattca gtcggttcaa ccatcagcgt acccgccacc gggaacgaca tcgtcggcgc
    15361 gtggaaaccg tagtcgatca ggcgcttggc aatatccagc tcgctgatgc cgatttcttc
    15421 tttcagcggg cgaatatcga gaatacattc gtgcgccacg cgaccgtcgc gaccggtata
    15481 cagcaccggg aaggcatcct gcaggcggct ggcaatatag ttggcgttga ggattgccac
    15541 ctggcttgct tttttcagcc cttctgcgcc catcatgcgg atgtacatcc agctgattgg
    15601 caggatagag gcgctaccga acggtgccgc agaaactgcg ccctgacggg ttaacatgcc
    15661 ttcgatttgc accacgctat gacccggtac aaacggtgcc agatgcgctt tcacaccgat
    15721 cggtcccata cccggaccac caccgccgtg cggaatgcag aaagttttat gcaggttgag
    15781 gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg ccaacctggg cgttcatgtt
    15841 cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg acttcacaca cttcacggat
    15901 cgtttcttca tacacgccgt gggtagaagg ataagtcacc atgatacagg agaggttatc
    15961 gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg atgttgccgt ttttatcaca
    16021 cgccacaacc accacctgca ttcctgccat atgtgcagaa gcggggttag ttccgtgcgc
    16081 agaagccggg atcaggcaga tatcgcgatg cccttcgttg cggctttcat gataatgacg
    16141 aatcgccagc aggcccgcgt attcgccctg tgcgccggag ttcggctgca tacaaacggc
    16201 gtcgtaaccg gtcagtttca ccagccagtc agccagctgc gcaatcatct gctgataacc
    16261 ttcagcctgt tccggcgggc aaaacgggtg cagttcggca aattccgtcc aagtgattgg
    16321 gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa cccagcggga tcatcgcctg
    16381 attcagcgcc agatctttac gctccagcga gtgcatatag cgcatcattt cggtttcgct
    16441 gtgatagcga ttaaacaccg gatgggtgag gatttcgtcg tcgcgcagca tcgcaggctg
    16501 gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg tcgatctcca gcccgtggtt
    16561 atcgcccagc agcacactga aaagctgcat tacgttttca cgcgtggtgg tttcatcaag
    16621 ggtgatcccc acggcgttca ggatatcgct acgcaggttg atttcagccg cttcggcacg
    16681 cgtcagtacg cccgctttgt cggctacttc cacacacaag gtgtcgaaat agtgcgcatg
    16741 gcgcagtttc agaccttttt gttgcaggcc cgccgccaga atatcggtca gacggtgaat
    16801 gcggttagcg atacgtttca ggccaaccgg gccgtgataa acggcataca ggctggcgat
    16861 gtttgccagc agtacctggg aagtacaaat gttggagttc gctttctcac gacgaatatg
    16921 ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg ccagctgcat ctttcgatac
    16981 accgataata cggcccggca ttgagcgttt gtattcatct ttcgccgcaa agaatgccgc
    17041 gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc gccgaaccaa aaacaatatc
    17101 cgcgccctgt ttacccggcg cagttaacag caccagcgcc ataatatcgg cggcaacgct
    17161 gaccacaatt ttgcgtgatt tcagttcgct aataagcgca gtgtagtcgt gaatttcacc
    17221 ggtagtgcct acctgctgta acagcacgcc gaagacgtcc tgatggtcga gcactttttg
    17281 cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca cgagtacgga ccacatccag
    17341 tgtttgcgga tgcacattgg aagccacgaa gaagcggttg gcatttttca gtttgctgac
    17401 gcgtttcgcc atcgccattg cttcggcggc agcggtggcc tcgtccagaa gagaagcaga
    17461 ggccatatcc agtccagtca aatccagcgt tacctgctgg aagttgagca gtgcttcaag
    17521 gcggccctgg gagacttcag gttgatacgg agtgtacgcg gtataccagc ccggattttc
    17581 cagcatgtta cgcaggataa ccggcggtag ctgcacggcg gtgtaaccca tgccgatgta
    17641 agacgtgaag cgtttattgc gactggcaat agccttgagt tctgccagtg cggcgtattc
    17701 ggtcgccggt gcgccaacct gcggtggtgt cgcaagctga atatctttcg gcacaatctg
    17761 gccggtcagc gcgtttaacg attgtgcacc aacggcattc agcatttctt gctgttgcgc
    17821 ggcgtccggt ccgatatggc gttcaataaa agcgccgctg ttttcaagct ggcttaacgt
    17881 ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt gtgaacctct ctccttacga
    17941 agagagtgag ggtgaggcat aaattttcct caccctgatc ctctcccgca gaagaggaat
    18001 aaagccgtta ctcatcttct aacaatgctt cgtatgcggt cgcatccagc agtgattcca
    18061 gttcgctttc atcgctagct ttgattttaa agatccagcc gcctgcatac ggttcgctgt
    18121 tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc cacgatttca ccgcttactg
    18181 gcgcataaat gtctgacgcc gcttttaccg attcggcaac cgcgcagtca tcgcccgcgc
    18241 taaccgttgc gcccacttcc ggcaggtcaa caaacaccat atcgcctaac agctcctgag
    18301 catgttcggt aataccaacg gtgtaagtgc cgtcggcttc tttacgcagc cattcgtgtt
    18361 ctttgctgta tttcagttct gctggtacgt tgctcatcaa tcaatctcca aaaaagtaaa
    18421 tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt gtcactttaa ccggcatttc
    18481 acggttgcga atttgcacaa tcgccgtttc gccaatacct tccggcacgc gcgccagcgc
    18541 aatgctgtaa cccagcgtcg gggagaaagt accgctggtg ataatgcctt catgctggtt
    18601 gccctgcgca tcggtaaagc gtaccggcag ttcattacgc agcacgcctt tttcggtcat
    18661 caccagacca accagttttt ctgtaccatg ctcacgctgc acttccaggg cttcacgacc
    18721 gataaagtca cgatctgtcg gttcccaggc gatagtccag cccatgttgg cggctaaagg
    18781 agaaatagtt tcgtccatct cctgaccata aagattcatg cccgcttcca gacgcagcgt
    18841 gtcacgcgcc cccagaccgc agggcttaac gcccgcttcc accagcgcac gccagaaatc
    18901 ggccgctttt tcattgggca gcgcaatttc atagcccgct tcaccggtat aaccagtggt
    18961 ggcaataaac agatcgccca cctgcacgcc aaagaacggt ttcatccctt ccaccgcctg
    19021 acgctgagca tcattaaaca gtgtggcagc ttttgcctga gcattcggcc cttgcacggc
    19081 aatcatggaa aggtcatcac gaacggtaat ttcgatgccg aaaggttcag cgtgttgggt
    19141 aatccaggag aggtcttttt cgcgggtggc ggagttaaca atgaggcgga agaaatcttc
    19201 agtaaagtag tagacgatga gatcatctat cacaccgcca gaggcattca acatccccga
    19261 gtaaagggct ttgccgcttt tggtgagctt cgccacatcg ttcgccagca gataacgcag
    19321 aaactcccgg gtgcggctgc cgcggagatc gacgatggtc atatgcgaca catcaaacat
    19381 tccggcatcg gtacgtaccg catgatgttc gtcgatttgc gaaccgtaat gcagcggcat
    19441 catccagccg tggaaatcca ccatgcgagc gccgcaaagc gtgtgttgtt cgtacaaagg
    19501 agtctgttgt gccatcttgt cctcattgaa taagcggggc tgacaacttt ttcatggtga
    19561 aattatcacc acaaaaccca gcatcggagc cactcccggt ccccaacgca atcgttctct
    19621 tttgcctgaa cttaccaccg aaacagactg ttaaccataa ggtaaaattg atcatcacat
    19681 tagcttatgg ttaaaaaatg caaaaatcgc gacagaataa aaaaccaaaa aatacaccaa
    19741 tttctataca aagatgatgt gatgagaaag tcaatttgaa taagacaata ttaagagcta
    19801 aaaaaatgtc aaaaaacact aaatcaaaaa atcatggcat tagaaaatat aatgcgaaaa
    19861 cggaggtgaa attagtttat ttcaaatgag gaaaatctcc cggcgaaaaa accgggagat
    19921 gaaagtgtga tgggtatcaa ataaacaaca gaggagaaat ttttaacgca gccattcaga
    19981 caaatcgttt aatcccattg cctggcggat aagttgcggc ttaacgccag gaagcgtgtc
    20041 ggccagtttc aaaccaatat cacgcagcag ttttttcgcc ggattagcgc cggaaaagag
    20101 atcgcgaaaa ccctgcatac cagccagcat cagcgccgcg ctgtgcttgc ggctacgttc
    20161 atagcggcgc aggtaaatgt actgcccgat gtctttaccc tgacgatgca accgtttcag
    20221 ttcagcaatc agctctgcgg cgtccataaa tccgagatta acgccctgcc ccgccagtgg
    20281 gtgaatagta tgcgcggcgt cgcccaccag cgccagacgg tgtgcggcaa actggcgcgc
    20341 ataacgcccc gtcagcggga acacctgacg cgcgctctca accttgcata agccaaggcg
    20401 attatcaaaa gcgatattta acgcgcgatt aaattcgtct tcacttgcct gctgcatccg
    20461 ctgcgcttcc tctggcgaca gtgaccagac aatcgagcaa agatgcggat cgctaagcgg
    20521 taaaaaggcc agaatgcctt cgccatggaa aacctgccgc gccaccgcat catgcggttc
    20581 ttccgtgcga atggtcgcta ccagcgcgtg atgctgataa tcccagaaag tcagcggaat
    20641 atctgctttg ttgcgcaacc aggaattagc accgtccgcg ccaatcacca gacgcgccgt
    20701 taacatgctg ccatctttca gcgtcaggaa ggtttcattt tctccccagg cgacctgctg
    20761 taattctgtg ggggccaaca gagtaatatc tgacgactgc tgcgctttgt tccacagcgc
    20821 gtagtgaatc actgagtttt caacgatgtg cccaagatgg ctatagccca tgctttgatc
    20881 gtcaaacgag atgtgaccaa aactgtcttt gtcccacact tccatgccgt gatagcaact
    20941 ggccctacga gcgagaatgt cctgccagac gccaagacgg gtgagtaatt tttcgctggc
    21001 ggcattgata gccgaaacgc gcagttgtgg tagtgcatct accgccagag gttccggtac
    21061 gcgctgctcc agtacggcaa cgcgtaagcc gctcccctgt aagccgcagg caaccgccag
    21121 ccccaccatg ccgccgccaa caatggctac atcaacactt tgcattgttt attccttaaa
    21181 accgccttca acgcgccacc caaccgaggg tgcgctgcgc cagcacatcg cgtgccgggg
    21241 tgaataattc catcgtcatc agtccgatgt tgcgcccgac aaccagcggt gcccaacggt
    21301 tggcaaaaag atgtaccagg ctgtccgtga cgccgatggt tgcttcgcga tcgctctgtc
    21361 gacgctgctg atagcggcac aatatgccgt aatcaccgat gtcttctccg cgctcatgcg
    21421 cctgagtcag ggtttccgca aggctcatga catctcgcat accgaggtta aacccttgcc
    21481 cggcaatcgg gtgcagagtt tgcgccgcat tgcccaccag cacagtgcga tgggtaatcg
    21541 gtctggcggc gcgggttaat gccagcggat aagcactgcg tttaccggcg tgggtaattt
    21601 gccccagtcg ccagccaaag gcagactgga gttcacggca aaacttctcg tcgctccacg
    21661 acagcacctc ttcgcgccgt tccagtggat gacaccagac cagcgaacag cgtccgtcag
    21721 acatcggcaa catcgccagc gggccatgtt gcgtgaagcg ttcaaaagcg cggccttcgt
    21781 gcgcaacaga agtagcaacg ttggcaatca ctgccagttg ttcgtaaggc tcctgctgcc
    21841 aatcaacgcc gcacgcggtg gctaacgctg aatgggtgcc atcagcggct atcagcacgc
    21901 ggcccgtcag catctcgccg ctctccagcg tcacttcaac gtgactctga gtacgggcaa
    21961 cgttagctac gcgatcgggg caatgcagca ttacgccagg tgcttttcgc agcaatgcaa
    22021 acagccgttg cccaacatta tgcaattcga caacctgccc cagcgccgcc agttggtaat
    22081 cttctgcggc gagggtgacg aatccagcat gaccacgatc gctgacatgc acggtagtga
    22141 tggcggttgc gcaatcagcc agagattgcc agacgccgat gcgcgccagt tgctgacagg
    22201 tacccgccgc cagcgctatc gctcgtccat caaagcccgg atgagcatgt gactctggcg
    22261 cagtcgcttc aatcaaatgt accggcagcg ccccgtgact taaccgggaa atagccagcg
    22321 ccagcgtcgc gcccgccatg ccgccaccga cgatgattac gctcattgct ttctcgcagc
    22381 agccatcaac gcttcgattt cttccggctt tttcaccacg ctggcggtga ggttttcgtt
    22441 gccggtttcg gtaatcacaa tgtcgtcttc aatacgaatg ccgataccgc gatattgttc
    22501 tggcacatcc gcatccggcg cgatatacag accaggctct acggtcagta ccatccccgg
    22561 ttccagaatg cgcgagcgat cctgaccata aacgccaacg tcatggacat ccagtcctaa
    22621 ccagtggcta aggccatgca taaagaaagg acgatgggcg ttctgagcga tcagttcatc
    22681 aacattacct ttcaggatgc cgagttttac caggccgcta accatgatgc gcaccacttc
    22741 accagtgact tccagaatgg aagttcccgg acgatacagg cgcaggctgg tttcgagaga
    22801 ctccagcaca atgtcgtaga tttcacgctg ggcctgggtg aatttgccgt tgaccgggaa
    22861 ggtgcgggta atatcgcccg cgtaaccttt gtattcacaa cccgcgtcaa tcaacaccag
    22921 gtcaccgtcg cgcagttcac tctcgttttc ggtgtagtgc agaatgcagc cgttttcacc
    22981 gctgcctaca atggtgttat aggaaggata gcgcgcaccg tggcggttaa attcgtggtg
    23041 aatttcgcct tccagatggt actcgaacat tcccggacgg catttttcca tcgcccgtgt
    23101 atgtgccatg gcggtgattt ctcccgcgcg gcggagtacg gcaatctctt ctggcgattt
    23161 gaacaggcgc atttcatgaa caacaggacg ccagtcgatc atcgttgccg gtgcggtgag
    23221 attttgccgc gaacctttac gcagtttttc cagcgcactg ttcacgattt catcagcata
    23281 tgcatattcg ccctgggcat ggtaaaccac atccaggccg ttaagtagtt gataaagttg
    23341 ctgattgatt tcgctgaatg ccagtgcgcg gtcaacgccc agtttctctg gcgcggcatc
    23401 ctggcctaag cgacggccaa accagatctc cgccgtcagg tcgcgaacgc ggttaaacag
    23461 aacgctgtgg ttatgagtgt catcgctttt aatcagcacc agcaccgctt ccggttcgtt
    23521 aaagccggtg aagtaccaga agtcactgtt ctgacgatag gggtattcgc tgtcggcgct
    23581 acgtgttact tctggtgcag caaaaatcag cgcggcgctg ccgggttgca tttgctccac
    23641 cagggcctga cggcgacgct gaaactcttg ccgggatatc tcactcataa cactctcctt
    23701 acgttttttg tttttagtgt agagtcggtt tttgtacttc tggcgcggtc ggttgcggat
    23761 gagtaaaggt gtcgtggcat aacagcgcgg caacacgaac gtattcgatg atctcttcaa
    23821 gcgacatttc aagctcttcc tgatcttcgt cttcgtcgta gcccagctgc gcgatgttac
    23881 gcagatcgtc gatggcttca ccggtttcgc cggtcacttt gtccagcttc ggttgcgtaa
    23941 cgccaagacc aagcaggaag tgattgaccc aacctgccag tgcatcagcc cgatcgaaaa
    24001 cgctgacatc atcgccatca ggcagataaa gctgaaaaag gaagccgtca tcctgcaggg
    24061 catcgctggt ggcagagtgc attttacgca gtgcctgtgc cagctcatga ccgaaagcca
    24121 tgccttcgtt cgtcaggtcg tgaagtagcg gcagccatga gctgtcatcg ttaccgccac
    24181 atatcatccc actgattaaa ccatgcatct cggcaggggt cagacccgtc ccttgttggt
    24241 tcagatactg gttcatttcg ttgtaaccag gcatttcgtt ctgtatagac ataagcattc
    24301 gtcgtcaaag ggaggaatat tcatgatatg ctaccacttt gggccctggt ggaccagaaa
    24361 agggcttgtc tcttctcatc agggtagcta tagcgtcgcc ccttcgcaga ccatgggtct
    24421 aaagacgaag gcagcgcagt caatcagcag gaaggtggca tgtctgcaca acccgtcgat
    24481 atccaaattt ttggccgttc actgcgtgtg aactgcccgc ctgaccaaag ggatgcgttg
    24541 aatcaggcag cggacgatct gaaccaacgg ttgcaagatc tgaaagaacg cactagagtc
    24601 acaaatactg aacagttggt cttcattgcc gcattgaata tcagctatga gttagcgcaa
    24661 gaaaaagcaa agactcgtga ctacgcggca agtatggaac agcgtattcg gatgctgcag
    24721 cagaccatag aacaagcgtt acttgaacaa ggtcgcatca ccgaaaaaac taaccaaaac
    24781 tttgaatgac acttttcggt ttactgtggt agagtaaccg tgaagacaaa atttctctga
    24841 gatgttcgca agcgggccag tcccctgagc cgatatttca taccacaaga atgtggcgct
    24901 ccgcggttgg tgagcatgct cggttcgtcc gagaagcctt aaaactgcga cgacacattc
    24961 accttgaacc aagggttcaa gggttacagc ctgcggcggc atctcggaga ttcccttctt
    25021 atctggcacc agccatgacg caactaccag agctcccact gacattatcc cgacaagaaa
    25081 tccgcaaaat gattcggcaa cgtcgtcgtg cgttaacgcc ggaacaacag ctggaaatgg
    25141 gtcaacaagc cgctacccgg atgatgactt atcccccggt ggtgatggcg catacggtcg
    25201 ctgtattcct ctcttttgat ggtgaactcg acacccagcc gctaatagaa caactctggc
    25261 gcgccggtaa gcgcgtatat cttccagttt tgcatccctt tagtgccggt aatttgctgt
    25321 tcctgaatta ccatccgcaa agcgaactgg tgatgaacag gttgaagatc catgagccaa
    25381 aactggatgt gcgtgacgtg ctaccccttt cccgattaga cgtgctgatc acaccgctgg
    25441 tcgcctttga tgagtacggt cagcgcctgg gaatgggcgg tggtttttat gatcggacct
    25501 tacaaaactg gcagcactat aaaacgcaac cggtgggtta tgcgcatgat tgtcagttgg
    25561 tggaaaaact ccccgttgaa gagtgggata tccctcttcc tgcggtggtt acaccgtcga
    25621 aagtctggga gtggtaaggg cgatacacct gcatcgccct gattgacatc gttgattctt
    25681 taacctaatt tagtgagtaa gggtaaggga ggattgctcc tcccctgtga ctgttaataa
    25741 gctgggaaac ttatgagtaa cagtacaatc agtatgatgg caagtcgcat cataaccctt
    25801 ctccttcaag ccctcgcttc ggtgagggct ttaccgttac agccccatgc tgcccagcta
    25861 tcgtaagctc tctttaaata accacaacgc attgatctac ctttaaatct tttcttcgag
    25921 cgtgctcgaa aaaccccatg taaaacagag ataatttttg ggaaaaagga tctattacgg
    25981 cagtaaacgt gggcgatgaa gtggctatca aaaagttcgg ttcagggcga tgcaccccgc
    26041 atcgccctga ttgacatcgt tgattctttg acctaattta gtgagtaagg gtaagggagg
    26101 atgactcctc ccttgagact gactgttaat aagctccgaa acttatcagt aacagtacaa
    26161 tcagtatgat gacaagtcgc atcataaccc ttctccttta agccctcgct tcggtgaggg
    26221 ctttaccgtt acagccccat gctgtcccgc catcgtaaat ccccattaaa taaacacaac
    26281 gcattgatct gactttgatt tattttctgg agcagactcg caaagtagaa tgcgcaacgc
    26341 ggcaacggtg tggagaaggg ataaaaaaac gggcaagtca gtgacctgcc cgttaatttt
    26401 cagagaaggg gaattagtac agcagacggg cgcgaatggt acccggaata gctttcattg
    26461 cctgcagcgc tttttcggca acgtcttcgt cagcttcaat atcaataact acataaccca
    26521 tctgggcgga agtttgtaga tattgcgcgg cgatgttgac gccctgctcg gcgaagattt
    26581 tgttcagcgc agttagcacg cccggacggt tttcgtggat gtgcatcaga cgacgaccac
    26641 catgcagtgg cagcgagact tccgggaagt tcaccgcaga gagcgttgag ccattgtcag
    26701 aatacttgat caatttaccc gcaacttcca agccgatatt ctcctgcgct tcctgagtcg
    26761 aaccgccaat gtgtggcgtc agaaggacgt tgtcgaattc acacagtgga gaggtaaatg
    26821 gatcgctatt ggtcgccggt tccgtcggga atacgtcgat tgccgccccc gccagatgtt
    26881 tgctcgccag cgcatcacac agcgccggaa tatccaccac agtaccgcgc gaggcattaa
    26941 tcagcagcga gccgggcttc attagcgaaa tctctttcgc gcccatcata tttttggtgg
    27001 acggattctc tggtacatgc agactcacca catcgctcat attcagcagg tcagaaagat
    27061 gctgtacctg agtggcgttg cccagcggca gtttgttttc aatatcataa aagtaaacat
    27121 acattcccag cgattcagcc agaatgccca attgcgtacc aatatgaccg tagccgatga
    27181 tacccagctt tttgccgcgc gcttcaaaag aacccgccgc cagtttgttc cacacgccac
    27241 ggtgcgcttt agcattggct tccggcacgc cgcgcaatag cagcagcagt tcgccaatca
    27301 ccagctccgc aacagagcgc gtatttgaga acggtgcgtt aaataccggg atcccgcgct
    27361 ttgccgccgc atccagatca acctggtttg ttccgataca gaaacagcca atagcgacca
    27421 gtttttctgc ggcgttgatc acgtcttcag tcagatgggt acgggatcgc aggccgatga
    27481 agtgggcatc gcggatggat tcttttaatt gttcatcatc cagcgcgcct ttgtgaaatt
    27541 cgatgttggt gtaaccagct gcacgaaggc tttccagcgc cttttggtgc acgccttcta
    27601 ccagcagaaa cttaatcttg tctttctcca gcgatacctt tgccatttac ccaatcctgt
    27661 cttttgaaat gttgtgtgcg gatttgcatc cgcctttcaa catatcaaaa aataatattg
    27721 cggcaatatg aacgtttgcg tcgcgatgtt gaggaaatat cacgcaaagg taattcttgg
    27781 atgaatatgc tggtttagag gatttattta gagcaatcga caattgcctg gtaaaagcgt
    27841 gacacatgtc accaaattta atgaagagaa tttttttaac gggggaggtt cccccgtcag
    27901 atcatttcac aatggttttg acaccgtcag gtgtgccaat cagcgcaacg tccgcgccac
    27961 ggttagcaaa caagccaaca gtcaccacgc caggaatcgc atttatggcg ttttccatcg
    28021 ctatcgggtc aaggatttcc atgccgtgga cgtcgaggat cacgttgcca ttatcggtca
    28081 ccacgccctg acggtattcc ggacgaccgc ccagtttcac cagctggcgc gccactgcac
    28141 tacgtgccat cgggataact tctactggca gcgggaattt acccagaata tcaacctgct
    28201 tggaggcgtc ggcaatacag ataaattttt ccgcaaccga agcaatgatt ttttcacggg
    28261 tcagcgccgc gccgccgcct ttgatcattt gcatgtggcc gttgatttca tctgcgccat
    28321 caacgtagat gccaaggctg tcgacttcgt tgagatcaaa aacgtgaatg ccgaggcttt
    28381 tcagtttttc agtggaagca tctgaactgg aaacggcccc ttcaatctgg cctttcatcg
    28441 tgccgagcgc gtcaataaag tgtgcggcgg tggaacctgt acctacacca acaatggtac
    28501 cgggctgaac atactgaagt gccgcccatc ctactgcttt tttcaattca tcctgcgtca
    28561 tgatcgtttc gcctgtggta tgaaatttca cacgcattat atacaaaaaa agcgattcag
    28621 accccgttgg caagccgggt ggttaactca tccataaaat atcgcgcaat ggcgggcatc
    28681 ccctttcgcc ccgcaaataa agcatacaac ggtctgggta tgccgctcca cggtgcgaac
    28741 agaggcaata acgccacgcc attcaacata accagggcgc gaagtcgcta agcgaaatgc
    28801 aatagcgcaa atccctgatc ttttaagctg cgcgtttaac cattgataca aatgttaccc
    28861 gccgacggct tcggtatatg caacctgaca caaaattgtg tcatagtgca ggaaaaagca
    28921 tttaccagga gcagacaaca gcaatgaaac gcccggacta cagaacatta caggcactgg
    28981 atgcggtgat acgtgaacga ggatttgagc gcgcggcaca aaagctgtgc attacacaat
    29041 cagccgtctc acagcgcatt aagcaactgg aaaatatgtt cgggcagccg ctgttggtgc
    29101 gtaccgtacc gccgcgcccg acggaacaag ggcaaaaact gctggcactg ctgcgccagg
    29161 tggagttgct ggaagaagag tggctgggcg atgaacaaac cggttcgact ccgctgctgc
    29221 tttcactggc ggtcaacgcc gacagtctgg cgacgtggtt gcttccggca ctggctcctg
    29281 tgttggctga ttcgcctatc cgcctcaact tgcaggtaga agatgaaacc cgtactcagg
    29341 aacgtctgcg ccgcggcgaa gtggtcggcg cggtgagtat tcaacatcag gcgctgccga
    29401 gttgtcttgt cgataaactc ggtgcgctcg actatctgtt cgtcagctca aaaccgtttg
    29461 ccgaaaaata ttttcctaac ggcgtaacgc gttcggcatt attgaaagcg ccagtggtcg
    29521 cgtttgacca tcttgacgat atgcaccagg cctttttgca gcaaaacttc gatcttcctc
    29581 ctggcagcgt accctgccat atcgttaatt cttcagaagc gttcgtacaa cttgctcgcc
    29641 agggcaccac ctgctgtatg atcccgcacc tgcaaatcga gaaa
//
LOCUS       DAJTNM010000038        28048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTNM010000038 DAJTNM010000000
VERSION     DAJTNM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.038"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..1188
                     /gene="pdeC"
                     /locus_tag="OO172_001809"
     CDS             <1..1188
                     /gene="pdeC"
                     /locus_tag="OO172_001809"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR5512019.1"
                     /translation="YRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQWGVY
                     DTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSYQRL
                     ITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQPIID
                     IKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGAYLA
                     THADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMTPII
                     LAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHIIE
                     LAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPARELT
                     RGQ"
     gene            complement(1191..1514)
                     /gene="soxS"
                     /locus_tag="OO172_001810"
     CDS             complement(1191..1514)
                     /gene="soxS"
                     /locus_tag="OO172_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5512020.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1600..2064
                     /gene="soxR"
                     /locus_tag="OO172_001811"
     CDS             1600..2064
                     /gene="soxR"
                     /locus_tag="OO172_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5512021.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2768..4117
                     /gene="ghxP"
                     /locus_tag="OO172_001812"
                     /gene_synonym="yjcD"
     CDS             2768..4117
                     /gene="ghxP"
                     /locus_tag="OO172_001812"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5512022.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAAFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4268..5917
                     /locus_tag="OO172_001813"
     CDS             4268..5917
                     /locus_tag="OO172_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5512023.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6071..7363)
                     /locus_tag="OO172_001814"
     CDS             complement(6071..7363)
                     /locus_tag="OO172_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5512024.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKENDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7541..9190)
                     /gene="actP"
                     /locus_tag="OO172_001815"
     CDS             complement(7541..9190)
                     /gene="actP"
                     /locus_tag="OO172_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5512025.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9187..9501)
                     /locus_tag="OO172_001816"
     CDS             complement(9187..9501)
                     /locus_tag="OO172_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5512026.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9701..11659)
                     /gene="acs"
                     /locus_tag="OO172_001817"
     CDS             complement(9701..11659)
                     /gene="acs"
                     /locus_tag="OO172_001817"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR5512027.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12052..13488
                     /gene="nrfA"
                     /locus_tag="OO172_001818"
     CDS             12052..13488
                     /gene="nrfA"
                     /locus_tag="OO172_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5512028.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDKQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13533..14099
                     /gene="nrfB"
                     /locus_tag="OO172_001819"
     CDS             13533..14099
                     /gene="nrfB"
                     /locus_tag="OO172_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5512029.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14096..14767
                     /gene="nrfC"
                     /locus_tag="OO172_001820"
     CDS             14096..14767
                     /gene="nrfC"
                     /locus_tag="OO172_001820"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5512030.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14764..15720
                     /gene="nrfD"
                     /locus_tag="OO172_001821"
     CDS             14764..15720
                     /gene="nrfD"
                     /locus_tag="OO172_001821"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5512031.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            15740..17458
                     /locus_tag="OO172_001822"
     CDS             15740..17458
                     /locus_tag="OO172_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5512032.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSMLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17451..17834
                     /gene="nrfF"
                     /locus_tag="OO172_001823"
     CDS             17451..17834
                     /gene="nrfF"
                     /locus_tag="OO172_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5512033.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17831..18427
                     /gene="nrfG"
                     /locus_tag="OO172_001824"
     CDS             17831..18427
                     /gene="nrfG"
                     /locus_tag="OO172_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5512034.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18769..20082
                     /gene="gltP"
                     /locus_tag="OO172_001825"
     CDS             18769..20082
                     /gene="gltP"
                     /locus_tag="OO172_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5512035.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20160..20849)
                     /locus_tag="OO172_001826"
     CDS             complement(20160..20849)
                     /locus_tag="OO172_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5512036.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20943..23090)
                     /gene="fdhF"
                     /locus_tag="OO172_001827"
                     /pseudo
     CDS             complement(20943..23090)
                     /gene="fdhF"
                     /locus_tag="OO172_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23288..24754)
                     /gene="mdtP"
                     /locus_tag="OO172_001828"
     CDS             complement(23288..24754)
                     /gene="mdtP"
                     /locus_tag="OO172_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5512037.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24751..26802)
                     /gene="mdtO"
                     /locus_tag="OO172_001829"
                     /pseudo
     CDS             complement(24751..26802)
                     /gene="mdtO"
                     /locus_tag="OO172_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26802..27740)
                     /locus_tag="OO172_001830"
     CDS             complement(26802..27740)
                     /locus_tag="OO172_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5512038.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADIALTKLHLEMTTVRAPFDGRIISLKTSVGQFASAMRPIFTLIDTRHWYVIA
                     NFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSI
                     NWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ"
     gene            complement(27759..28034)
                     /locus_tag="OO172_001831"
     CDS             complement(27759..28034)
                     /locus_tag="OO172_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512039.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         6381 a         7111 c         7562 g         6994 t
ORIGIN      
        1 taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg gggaaattat
       61 gtggcggtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc gacattgcaa
      121 tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga ggcctcggca
      181 gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa tggctattta
      241 tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac ttcatatcaa
      301 cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg tattttgggg
      361 agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc tcccaaacgt
      421 aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc aataatcgat
      481 atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg tgagcagggg
      541 caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat gatagaacag
      601 ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct ggcaacacat
      661 gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc acggttgata
      721 gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat taaatttgaa
      781 gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct ggctttccgc
      841 caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa cttgcataac
      901 cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga aacgctgacc
      961 acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca cagcctgggg
     1021 ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct gcgcaaacgc
     1081 ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca ggtgtttatg
     1141 caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa ctacaggcgg
     1201 tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga gaaggtctgc
     1261 tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt ggtgcgcaac
     1321 tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt ctgatgcgtc
     1381 accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt tgcgacgaca
     1441 tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc ctgaataatt
     1501 ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc atctgttggg
     1561 gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa attaccccgc
     1621 attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc ggtatcggcg
     1681 ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg caatcagcgg
     1741 cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca gcgtattggc
     1801 attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca tacgttaagt
     1861 gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg gcgcattcat
     1921 accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg cctttcgcgc
     1981 agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac cggcgcacgc
     2041 ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat cgttaactat
     2101 gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca ccctaaccct
     2161 ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca aacacaaaac
     2221 taaagcgcca caagggcgct ttagtttgtt ttccggtctt tgtctttctc tctatcccgc
     2281 tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg tggtggaggt
     2341 tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca gtgtaaaagt
     2401 atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca atccagaacg
     2461 gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc gcatcaattg
     2521 cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc ggttgaaaaa
     2581 gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta tgcgcggagc
     2641 taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctggagcag gaagataacg
     2701 tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc tcaggggatg
     2761 ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct ggtttaaaat
     2821 ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa cgtttctggc
     2881 gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct tcccgcctgc
     2941 ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga tgggtctgtg
     3001 ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta ccgcattcag
     3061 cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgcgt tcctgatggg
     3121 ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc gcaacttgcc
     3181 gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc tcattgccgc
     3241 taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg cgctgggtga
     3301 tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca tcggcttgga
     3361 aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa ttgtcggttt
     3421 gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat tgagcgatga
     3481 aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc ctgtagtcct
     3541 gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa ctatccgcgc
     3601 tgtcgccggc caggcgaacc tgctggataa agatgggcag atcatcgacg gcgggaaagc
     3661 actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag ctccggcagc
     3721 ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt tgacggctat
     3781 caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt acctcgttcc
     3841 ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga gcaacgtggc
     3901 gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg cggtattcat
     3961 cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc tggtgattgg
     4021 tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta tcgccgtggc
     4081 gctggtgacc ttctatgcgg ggggctgggc tatctaatct ttcatcggat tctgaaaacg
     4141 ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt tgcgatattc
     4201 tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca tcgcaaacag
     4261 ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct cgctgtccgg
     4321 ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa tcgccatcgg
     4381 tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg aactcttttt
     4441 agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga cccgtgaatt
     4501 tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg tcaccgtggt
     4561 cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc ccgcctttgc
     4621 gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg ttggcgaagg
     4681 gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga atgacgcttc
     4741 cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga tcttcactgt
     4801 cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg ccggttttgt
     4861 ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg gtgatgaacc
     4921 cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga ttgccgaaca
     4981 tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca cccgctccgg
     5041 tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct gggcgatgct
     5101 ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc cgggtattct
     5161 ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga tgctgtttac
     5221 cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc tgtggacgat
     5281 gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt cgtggaccac
     5341 acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc tggccggtgt
     5401 gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct atgagctggt
     5461 gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga tgttgcctat
     5521 tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag aacgtattgc
     5581 gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg agcgtctggc
     5641 ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt ctcgcgtcat
     5701 tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg tgcaggaaga
     5761 gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg aactgtacca
     5821 cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc acgatctcga
     5881 tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg taaccaggcc
     5941 cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct cgggcgaaca
     6001 ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt ggctgttctt
     6061 cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata gcgactgcat
     6121 taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct tcaaagaatt
     6181 tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca cggagaatgg
     6241 aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga cgggtattaa
     6301 acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa ttggcattat
     6361 taaaattaac attagccata ttagagccgg tacatataac catttcgagg aatgtatttg
     6421 tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg attgctttgt
     6481 tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt ttagagaaac
     6541 taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga tttacctcag
     6601 ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata ttggctctat
     6661 tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga gttccgtcag
     6721 ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa agtatttttt
     6781 ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa atagcgtttt
     6841 gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc acgataccct
     6901 tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt tcgatagtat
     6961 tattatggac ggcgacatta tttataaaat ataccacctt aatggataca tcattttctt
     7021 tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca tataacgcta
     7081 tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt cctgatggta
     7141 aactatcata aattcttttc attttctgaa tcaagcctat cactttttca tgatcatcta
     7201 ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt ttgctgtaat
     7261 tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta tcgttaatct
     7321 ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat ccttataatt
     7381 aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc aaaaatcgct
     7441 cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat gttggagagg
     7501 aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg cggccttgct
     7561 caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca cgctcgcgcg
     7621 ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat gccacgctga
     7681 tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg aggatctgta
     7741 cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcccc agccagccgc
     7801 ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga atgatcggga
     7861 agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg ttctgattct
     7921 caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg atttttgata
     7981 cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac aagtcatgcg
     8041 aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata gtggcgaaag
     8101 caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc aggtgaacgg
     8161 ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc ggattcgcac
     8221 caacaagcat gatcgcgccg aagccgataa taaaggtcag aatatagaag tagcccataa
     8281 atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact gtaaagaagc
     8341 gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc agagagagcg
     8401 cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct ttcgggtgta
     8461 ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc atcaccataa
     8521 aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt acccaggtgg
     8581 tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc agcaccaccg
     8641 caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct accatctgag
     8701 cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata cgaatcggcc
     8761 cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg ttacgcagac
     8821 gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac gagtagatca
     8881 gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag gcggcggaca
     8941 tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg cctgcggtgt
     9001 agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg agcgtaaaca
     9061 cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc tctacggccc
     9121 cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc gccgtcagaa
     9181 ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt cgaattcgcc
     9241 gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca ctccaacacc
     9301 aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca gccagccggg
     9361 cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa tcgacaggat
     9421 ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt cttctatccg
     9481 ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat tgttttaatt
     9541 cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg taggccggat
     9601 aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc gtcttatcag
     9661 gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc atcgcgatag
     9721 cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc gaggtatcgc
     9781 ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata attttgccgg
     9841 agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc agcgggccaa
     9901 tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt tcctccccgt
     9961 gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga atacctacta
    10021 cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct gccgtcccca
    10081 gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag taatagccat
    10141 cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg gaaaaatagg
    10201 tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc caggagtcgg
    10261 tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta tcgaccagcg
    10321 ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg gtagcgccag
    10381 gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg accaccggac
    10441 atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta attggctcgc
    10501 ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct ttatcgcctt
    10561 ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc tgatgcttgt
    10621 ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca aacatcagcg
    10681 tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta acccagccca
    10741 catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat ttaaaggtca
    10801 gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt tttccggtag
    10861 aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc gcctggtgct
    10921 gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc tgccagtcga
    10981 tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg ttcgggtttt
    11041 ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc acgccttcgt
    11101 cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg gctttcggcg
    11161 agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc agcatcgcaa
    11221 ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt ttaatgccca
    11281 gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta tagctgatat
    11341 gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg ccgttttctt
    11401 gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg taccatttaa
    11461 tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt ttgatccagt
    11521 cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat tgttgataca
    11581 tcgcctcgta ctgctgaggg tttatcaggc aacggtctgc gatgttggca ggaatggtgt
    11641 gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat atgtggcata
    11701 agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag atcacgcaaa
    11761 agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca aaagttgatt
    11821 aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat actaactaac
    11881 tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca cagtgtggtt
    11941 atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta ctgtcgtgaa
    12001 aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc tatgacaagg
    12061 ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt tttcacttct
    12121 gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc gaagaatgaa
    12181 acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc ggaacagtca
    12241 gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc ggggtatccc
    12301 ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga tgtgcgtgaa
    12361 accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat ggcgtgctgg
    12421 agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga tggctacttc
    12481 cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg tgccgactgc
    12541 cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct ttcccgtccg
    12601 tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg acgtttcgac
    12661 aagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg caaaaacaaa
    12721 gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca gtattacgac
    12781 aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct gaaagcgcag
    12841 cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt gacctgtatc
    12901 gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga ccataaaatt
    12961 ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca ggataaagct
    13021 gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa aatcaaggtt
    13081 gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg cgctggatgc aggcgcgacg
    13141 gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg ttgggatctg
    13201 gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg gatgctcggt
    13261 acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct ggcgaccaaa
    13321 ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc ccagcaggcc
    13381 attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa aacggtgatc
    13441 ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc ccgttccgcc
    13501 tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg ttattaactg
    13561 ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct acccctgcgg
    13621 tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat gccgcctgtc
    13681 tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc gttatcaacc
    13741 cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca ccgcaacacc
    13801 gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc ggagagcaga
    13861 acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc tggccgcacg
    13921 atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca cagcaagata
    13981 cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc cacagcgatc
    14041 agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag cagccatgac
    14101 ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca gcggcaccgc
    14161 agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca tggtgcatga
    14221 cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag tgaacaaggt
    14281 gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg aatttcctga
    14341 tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc cgtgcgttga
    14401 cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg atgttaatcc
    14461 tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg tgcgctttat
    14521 ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca atttgcaggc
    14581 cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt ttggcaatct
    14641 ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt accgctacaa
    14701 gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg gggaggtgag
    14761 ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg gccgattgcc
    14821 atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt gctgttacgt
    14881 cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac gctgattgtc
    14941 gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac aagaccgtgg
    15001 accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat gggggtgatg
    15061 ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt tgaatatgat
    15121 ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa ggttctgagc
    15181 cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc ggtgctgctg
    15241 ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct caataacccg
    15301 agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt ggcgctgatc
    15361 gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt tgttcaccgg
    15421 atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt tgtcggtctg
    15481 gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg cggtttctgg
    15541 acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat gttgctcaaa
    15601 ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggcgtgtgg ggccagtctg
    15661 gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac ggtggcgtaa
    15721 gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc tggcgttgtt
    15781 gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag tgcggttgcg
    15841 ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg cgctcctgct
    15901 gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg tcatttatgt
    15961 cgcccaacat agctacagca tgctgtcgtg ggaactcaaa ctggcggcgg tgtggggcgg
    16021 tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg cgctgtttgc
    16081 ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg ttttatctct
    16141 catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg tgcggatatt
    16201 tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg gtcttatctt
    16261 tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga gcgtggcgct
    16321 ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct ggcgctgggc
    16381 gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt gggcctactg
    16441 cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct ctttattacc
    16501 ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc gggggatttt
    16561 ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc tgggcacctt
    16621 aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata acgtccgcgc
    16681 cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc tgtatggctg
    16741 gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg aaatgttaat
    16801 cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg gaacgcttta
    16861 tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc cgtattttaa
    16921 ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg cgacgtttgt
    16981 ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc tgttatttgc
    17041 cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt tacagccggg
    17101 tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc tgcaagccag
    17161 aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc aacgcattgg
    17221 tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga tggaaccgtc
    17281 aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa ccagagcgga
    17341 tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg ggggaggatt
    17401 gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg atgaataaag
    17461 ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt gtagacacct
    17521 ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc cagttacgtt
    17581 gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg gtcagtatgc
    17641 gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc attggctgga
    17701 tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt cagacgctgg
    17761 tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc tggcgagtga
    17821 gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac tcacgatatt
    17881 gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg cggtacgtgc
    17941 ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa ccccggaagc
    18001 gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg aacaatgggc
    18061 gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc tggcgtatcg
    18121 tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg cgacggtgct
    18181 ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg acaaagccct
    18241 cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg cgtttatgca
    18301 ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca actcaccccg
    18361 gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc agcggagatc
    18421 ggattaatca tctgttgatg aagattatgt cattgagctg cataaaaaat aatcgaatga
    18481 acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat aaaaatcttt
    18541 ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg cattaatgca
    18601 gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata ctgatcataa
    18661 gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt atgtcacgtt
    18721 ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat gaaaaatata
    18781 aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct cctgggaagc
    18841 tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct ctctccggcg
    18901 ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat ctccacgctg
    18961 gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg cgcgaaaacc
    19021 attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac tctggcgaac
    19081 gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga tatctcgaaa
    19141 tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg cacgattttg
    19201 tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct gccgatcatc
    19261 tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca tcgtgaaccg
    19321 ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca catggtgatg
    19381 cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa ctttggtttc
    19441 tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat tctgttcttc
    19501 gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg gagcctgatt
    19561 cgtattctga aagatgagct gattctggcg tactccactg ccagctctga aagcgtactg
    19621 ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac cagcttcgtg
    19681 gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag tattgccgct
    19741 attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat cattctggtg
    19801 ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc gtttgtggtg
    19861 ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt tattgctggt
    19921 gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa tgcgctggcg
    19981 gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct ggcttatgag
    20041 cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat tgccggggat
    20101 atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc aggcctacct
    20161 taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc catacagctc
    20221 aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc ctcaccgttt
    20281 aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc agagctgcgt
    20341 ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt ggcgtaaatc
    20401 aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc cgaggcgttt
    20461 tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt taccagaata
    20521 tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc tgcaagaact
    20581 tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc caacagcgcg
    20641 caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc tttgctcaaa
    20701 tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc cccggcctct
    20761 gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa gaatgtcaaa
    20821 aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag ggagtaacca
    20881 gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta gaaaggactg
    20941 tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc gtcaatcagg
    21001 tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca gtatttgtat
    21061 tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca ggcaccaatc
    21121 caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac ctgcgcacgg
    21181 gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc aatacccaga
    21241 cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag cgccgccagt
    21301 gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg caccgttgac
    21361 agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc gcaggtgaag
    21421 aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga agtcccctga
    21481 tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc cattttctcg
    21541 taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat ctcctgggtg
    21601 ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat ttgccagtcc
    21661 gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc acggtcagcc
    21721 gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac atccgccgcc
    21781 gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc ttcaaaggct
    21841 ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat aatgtacgcg
    21901 gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata gccggtatga
    21961 gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc cggatctttc
    22021 acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc accctgcacg
    22081 ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt accggtcagc
    22141 atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg ggttacaccc
    22201 atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc ctgacgaatc
    22261 tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc ttcaacgatt
    22321 ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc gtacagattt
    22381 tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc gtttttcagt
    22441 gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca gacgataatt
    22501 ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg ggaatccgcc
    22561 gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat agcattgctc
    22621 attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg tcagacacga
    22681 gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg cattacatag
    22741 ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc cggaccgtac
    22801 ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc tcaggaaaca
    22861 ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg cggggtcagg
    22921 atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag ggtaccctgg
    22981 ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac gaccaggttg
    23041 attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat cggtctcgct
    23101 ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt tatgccacat
    23161 tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc tgacgaaaaa
    23221 caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc atcacgcggc
    23281 agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc agcgatttca
    23341 tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac atctcttcgg
    23401 caagtactgg caaccgggct tcagtggcct gtaagcggct ggttaagccg cgctgatagg
    23461 cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc tgcatttctc
    23521 gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc accgcgttca
    23581 gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg ccttcgagat
    23641 tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg aagttgaact
    23701 ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg aaaaacgctt
    23761 tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct aatgacgcct
    23821 gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca taagagagtg
    23881 tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc ggcatatcgc
    23941 tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt tgccctttga
    24001 cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg aaaggcactt
    24061 gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa tcaatcacat
    24121 cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg taataaagct
    24181 gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc gcggcattat
    24241 gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca agatccagtc
    24301 cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc agtgcgggcg
    24361 catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg ttaagcatcc
    24421 ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc gattgcgctt
    24481 tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc cgttggatca
    24541 gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc cagccggagc
    24601 tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt tgatgaggcg
    24661 cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg agaatgctgc
    24721 acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg cctgttccgt
    24781 ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct ggcagacgtg
    24841 ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct cttcaagcgt
    24901 tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct ccagggcgtc
    24961 ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg ccgaattcca
    25021 ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt gcgaacgttc
    25081 aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc gttggcacat
    25141 ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat aagtgcgcag
    25201 agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac ccagcgcgcc
    25261 agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg tgtaaatcat
    25321 cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt ccaccaggtc
    25381 gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca ccatctgtgt
    25441 gccgatataa gaagagcgtt cagagctggt ggcaatctat gcgcccaaca ggaaaatcgg
    25501 tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca gtagcgtgaa
    25561 taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca tcttctggta
    25621 cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg tgtgaatgcc
    25681 ttcccagtcc acgccgctgt aaaaggtgta gcagatcaaa caggcgagca atgtttttac
    25741 cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg gcggtttggc
    25801 ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg tctggcagat
    25861 attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc gccagtcgct
    25921 ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg aggctaattt
    25981 ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt agcgattcag
    26041 cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc tttgagttcg
    26101 ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca gcgcctctct
    26161 ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa tggcatcatc
    26221 aagcagatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa accacagcac
    26281 gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc acagcgttaa
    26341 gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt aaatggcgac
    26401 aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa acatgcagcc
    26461 catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg accatttgta
    26521 gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga tcgccacaaa
    26581 tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg ataacgccac
    26641 aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta cccagagttg
    26701 cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa agaacgccag
    26761 cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg ctcaagatta
    26821 gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac catgattttg
    26881 accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt tggcaggccg
    26941 cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc cactttaccc
    27001 tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc tgagcgaata
    27061 tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt gtcgatgagg
    27121 gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag ggaaatgatc
    27181 cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt cagggcaata
    27241 tccgcttctg cggcgcgctg cgccgttctt gcacggtcaa cgtcttctgc tgagacaaaa
    27301 ccctctttca gtaatggctc ggtgcggcgt aatgtatctg tggcctgttt cgcggcggca
    27361 cgggcttttt ctaccgtggc attaaccgag tcggcaccaa actgttgggc gtcaacgcta
    27421 cgctgggtga gcataatttg cttatccagc gccgcgaggg aggcttcggc tttcgccagg
    27481 ttggcttcgt acgggcgcgg gtcgatgcgg aacagcaaat cgccctgttt caccgcctgg
    27541 ttgtcggtga ccgccagttc tacaatgcga ccgctgactt ccggcaccac atcaatggta
    27601 tctgctgacg cgtaagcgtc attagttgat ggcgcactgt ctacgcgcca gataacgaaa
    27661 acaagggcaa ccagcgccaa cgcaaccact aacagagcag ggaatttact gcgaggtgct
    27721 tttttcggcg tactttccat gagcgcgtct caaatagatt agaaaaatgc cagccagaac
    27781 agcatggcgt agagagcaaa aagggcggta taaataattc cgacaaatgc cagattgata
    27841 tttgcccgct gaataactcg cctcgtgatg agcgtcaaaa taaggctggc aatggcgcag
    27901 aaaaaccagc tgggataata agcgccgatc accgggattg ccggagataa tgagcatccc
    27961 gaaaccatga agacgggaat tatccaggct gtttttttga ggtgtatcgc catgcgagag
    28021 agaactgttg gcatattaaa tacatcct
//
LOCUS       DAJTNM010000039        27944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTNM010000039 DAJTNM010000000
VERSION     DAJTNM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.039"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(17..307)
                     /gene="ubiK"
                     /locus_tag="OO172_001832"
     CDS             complement(17..307)
                     /gene="ubiK"
                     /locus_tag="OO172_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5512040.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
     regulatory      429..577
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            681..1334
                     /gene="ribB"
                     /locus_tag="OO172_001833"
     CDS             681..1334
                     /gene="ribB"
                     /locus_tag="OO172_001833"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5512041.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1595..1765
                     /locus_tag="OO172_001834"
     CDS             1595..1765
                     /locus_tag="OO172_001834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512042.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1823..2596)
                     /gene="zupT"
                     /locus_tag="OO172_001835"
     CDS             complement(1823..2596)
                     /gene="zupT"
                     /locus_tag="OO172_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5512043.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2739..3527
                     /gene="ygiD"
                     /locus_tag="OO172_001836"
     CDS             2739..3527
                     /gene="ygiD"
                     /locus_tag="OO172_001836"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5512044.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3565..4725)
                     /locus_tag="OO172_001837"
     CDS             complement(3565..4725)
                     /locus_tag="OO172_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5512045.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(4731..5402)
                     /locus_tag="OO172_001838"
     CDS             complement(4731..5402)
                     /locus_tag="OO172_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5512046.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5550..7031)
                     /gene="tolC"
                     /locus_tag="OO172_001839"
     CDS             complement(5550..7031)
                     /gene="tolC"
                     /locus_tag="OO172_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5512047.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQACLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7236..7865
                     /gene="nudF"
                     /locus_tag="OO172_001840"
     CDS             7236..7865
                     /gene="nudF"
                     /locus_tag="OO172_001840"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5512048.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7866..8288
                     /locus_tag="OO172_001841"
                     /pseudo
     CDS             7866..8288
                     /locus_tag="OO172_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
     gene            8313..9140
                     /gene="cpdA"
                     /locus_tag="OO172_001842"
     CDS             8313..9140
                     /gene="cpdA"
                     /locus_tag="OO172_001842"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5512049.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9140..9721
                     /gene="yqiA"
                     /locus_tag="OO172_001843"
     CDS             9140..9721
                     /gene="yqiA"
                     /locus_tag="OO172_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5512050.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9750..11642
                     /gene="parE"
                     /locus_tag="OO172_001844"
     CDS             9750..11642
                     /gene="parE"
                     /locus_tag="OO172_001844"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5512051.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11690..12004)
                     /locus_tag="OO172_001845"
     CDS             complement(11690..12004)
                     /locus_tag="OO172_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5512052.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12035..12616)
                     /gene="mdaB"
                     /locus_tag="OO172_001846"
     CDS             complement(12035..12616)
                     /gene="mdaB"
                     /locus_tag="OO172_001846"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5512053.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            12935..13267
                     /locus_tag="OO172_001847"
     CDS             12935..13267
                     /locus_tag="OO172_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5512054.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(13313..14662)
                     /gene="qseC"
                     /locus_tag="OO172_001848"
     CDS             complement(13313..14662)
                     /gene="qseC"
                     /locus_tag="OO172_001848"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5512055.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14659..15318)
                     /gene="qseB"
                     /locus_tag="OO172_001849"
     CDS             complement(14659..15318)
                     /gene="qseB"
                     /locus_tag="OO172_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5512056.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15470..15862
                     /gene="ygiW"
                     /locus_tag="OO172_001850"
     CDS             15470..15862
                     /gene="ygiW"
                     /locus_tag="OO172_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5512057.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15915..16397
                     /locus_tag="OO172_001851"
     CDS             15915..16397
                     /locus_tag="OO172_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5512058.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            16943..19201
                     /gene="parC"
                     /locus_tag="OO172_001852"
     CDS             16943..19201
                     /gene="parC"
                     /locus_tag="OO172_001852"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5512059.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19435..20172
                     /gene="plsC"
                     /locus_tag="OO172_001853"
                     /gene_synonym="parF"
     CDS             19435..20172
                     /gene="plsC"
                     /locus_tag="OO172_001853"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5512060.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20247..21659
                     /gene="ftsP"
                     /locus_tag="OO172_001854"
     CDS             20247..21659
                     /gene="ftsP"
                     /locus_tag="OO172_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5512061.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21770..23987
                     /locus_tag="OO172_001855"
                     /pseudo
     CDS             21770..23987
                     /locus_tag="OO172_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
     gene            complement(24030..24287)
                     /gene="yqhH"
                     /locus_tag="OO172_001856"
     CDS             complement(24030..24287)
                     /gene="yqhH"
                     /locus_tag="OO172_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5512062.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24338..25264)
                     /locus_tag="OO172_001857"
     CDS             complement(24338..25264)
                     /locus_tag="OO172_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5512063.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNNNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            complement(25464..26291)
                     /gene="dkgA"
                     /locus_tag="OO172_001858"
     CDS             complement(25464..26291)
                     /gene="dkgA"
                     /locus_tag="OO172_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5512064.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            complement(26396..27559)
                     /gene="yqhD"
                     /locus_tag="OO172_001859"
     CDS             complement(26396..27559)
                     /gene="yqhD"
                     /locus_tag="OO172_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5512065.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKAAR"
     gene            27753..>27944
                     /locus_tag="OO172_001860"
     CDS             27753..>27944
                     /locus_tag="OO172_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5512066.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYIN"
BASE COUNT         6535 a         6939 c         7425 g         7045 t
ORIGIN      
        1 cattaaagac aaatgtttac agcgttgggg ggagagtctc tggatctggc tgctttttga
       61 tctcagtact gcgattttcc agttcgctga ttctttgttc aagcagcgcc agtttctcac
      121 gagtccgtaa caggacttgc gtttggacgt cgaactcttc acggcttaca aggtcgaggc
      181 gcgtcagctg agcttgtagg gtttggcgga tttttttctc cacatcttcc ccgaactccc
      241 tgattccttt aggcattgat tcgtgaacct ggcgagcgat ttgctcaatt tttttcgggt
      301 caatcattgt agtttccctg tactgatagg tgttgagtac cattgtagtg cgataagggt
      361 aagtcataaa ccagaattat gtgaagctat gcgttgctgc cgctaatcat tagagttata
      421 gtgaatccgc ttattctcag ggcggggcga aattccccac cggcggtaaa tcaactcagt
      481 tgaaagcccg cgagcgcttt gggtgcgaac tcaaaggaca gcagatccgg tgtaattccg
      541 gggccgacgg ttagagtccg gatgggagag agtaacgatt ctgtcgggca tggacccgct
      601 cacgttattt tggctatatg ccgccactcc taagactgcc ctgattctgg taaccataat
      661 tttagtgagg tttttttacc atgaatcaga cgctactttc ctcttttggt acgcctttcg
      721 aacgtgttga aaatgcactg gctgcgctgc gtgaaggacg cggtgtaatg gtgcttgatg
      781 atgaagaccg tgaaaacgaa ggcgatatga tcttcccggc agaaaccatg accgttgagc
      841 agatggcgct gaccattcgc cacggtagcg gtattgtttg cctgtgcatc actgaagatc
      901 gccgtaaaca acttgatctg ccaatgatgg tagaaaataa caccagcgcc tatggcaccg
      961 gttttaccgt gaccattgaa gcggctgaag gtgtgactac cggtgtttct gccgctgatc
     1021 gtattacgac cgttcgcgca gcgattgccg atggcgcaaa accttctgat ctgaatcgcc
     1081 cgggccacgt tttcccactt cgcgcgcagg caggtggtgt actgacgcgt ggcggtcata
     1141 ctgaagcgac tattgatttg atgacgctgg caggctttaa accggctggt gtgctgtgtg
     1201 agctgactaa tgacgatggc acgatggcgc gtgcaccaga gtgcattgag tttgccaata
     1261 aacacaatat ggcgctcgtg actattgaag acctggtggc ataccgtcag gcacatgagc
     1321 gtaaagccag ctgaaaaccg ctgcttaatt actgccttaa tcaagaaacc gaagttgtag
     1381 ctggcttcgg tttttatttt gccctgctat gcaagatgtt aattccgctt ctctttctat
     1441 gagaaaattt cattaatatc aggcattctt tttcattatg atcaaaatca tataattgtt
     1501 ccgttattat gcgaccatat gaatatatcc gaaaaaggat atgtgaaatt tatgcgctaa
     1561 atgtttagta ttgttaaata ttaagggtaa aaggatgttt atcgcctggt actggattgt
     1621 attgattgct ctggttgtgg tgggttattt cctgcatttg aaacgttatt gtcgggcgtt
     1681 tcgccaggac agagacgcac tgcttgaagc gcggaacaaa tacttaaaca gtacgagaga
     1741 agagacagcc gaaaaggtag aataaggctt tcgtgaagct ggcagaagtt gtccatgtgc
     1801 tggacaactt ctgatgttgt ttttaaccaa ttcccgccgt ttgtagcagc actaaactga
     1861 atcccatcac tgacattcca catagtacgc catagctggg gttattatta gggtcaatct
     1921 ctttggcgag cggcattaat tcatcaaccg agagcgccac cataattcct gcaaccgccg
     1981 ccatgattgc cgccatgacc accggggaaa tcatgctacc gaggattaac cacgccagca
     2041 caccaccaag aatttctgct agtccagaaa tccccgccca cagaatcgcg gtgcgtttag
     2101 accccgttgc cgcataaacc gggcctgcca ctgccagacc ttcagggata ttgtgcaacg
     2161 cgacggccag tgcgatgcca aatcccagct ccaggttgct gctcgccgtg acaaaggtgg
     2221 caatcccttc cgggaagtta tgcagactga tgccgagagt gagcagaatg gctgtgcgct
     2281 tgatcgattt tggcaacagc tgcaccgatt tttgcattaa atcctgcgga tgcgcatgcg
     2341 gcaacatgcg gtccaggcca aagtagccaa gcagaccgaa gataaacatt ccataaccca
     2401 acaccggcga cattccttca gctgctagtg cggcaggaag catttccatt aatgagatga
     2461 gcaacatgat ccccgccgca aaacctagcg aaaacgccag taagcggttc gagggttttt
     2521 gtccgagaac gccgagaaac gcgccaataa acgtggctgc ccccgccagt atggtcagaa
     2581 tgagaggtac tgacatcgac aactccttat aaatcttcac cttcaggcag atcgtcaaaa
     2641 taactgatga tcatcatcat tgttatccgg gttcttactc cgccgaacaa gcgtattgtg
     2701 aggatatcaa aatgacacct ttagttaagg atatcatcat gtcttcaaca cgtatgccag
     2761 cattgttttt aggtcacggt agtccgatga acgtgctgga agataatttg tatacccgca
     2821 gctggcagaa gttggggatg acattgccac gcccgcaagc gattgtggtg gtttcggctc
     2881 actggtttac ccgtggaaca ggagtgaccg cgatggagac gccgcccacg attcatgact
     2941 ttggtggctt cccgcaggcg ctgtacgata cgcattatcc tgctccgggt tcgcctgcgc
     3001 tggcacagcg tctggttgag ctgttagcgc cgatcccggt gacgctggat aaagaagcct
     3061 ggggctttga ccacggctcg tggggcgtgc tgattaagat gtatcctgac gccgatatcc
     3121 ctatggtgca gttgagtatc gacagtagca aacctgccgc ctggcatttc gaaatggggc
     3181 gcaaactcgc ggcgttgcgt gatgaaggaa taatgttggt cgccagcggt aacgtggtgc
     3241 ataacctgcg cacagtgaag tggcacggtg atagttcacc gtatccgtgg gcgacgtcgt
     3301 ttaatgagta tgtgaaagcg aatctgacgt ggcaagggcc ggtagagcag catcctctgg
     3361 tgaattatct cgaccatgaa ggtggcgcgt tatcgaaccc aacgccagag cactatctgc
     3421 cgttgttgta tgtgttaggt gtgtgggatg ggcaggagcc aattaccatt ccggtcgatg
     3481 gtatagaaat gggcagttta agtatgctgt cggtgcagat aggctaacaa ttaccggatg
     3541 gcgagtgcca tccggtatcg tggcttattc aacaaaaata tgtggataaa accgagacat
     3601 atcctgggtg atcaacgcac ggtcttcacg aatgccaatt ccggcaggtt gatcgttcac
     3661 cagccagcta ccaatcagca tatagctgtc gccgaatttc ggtaacgggt ggaattgctg
     3721 aacaatcatc ccttcttcgc catacggacc ttccgctgct tcaatggttt tgccgttctc
     3781 aatgatcgac acgtttgcgc cttcacggga gaagatcggt ttaaccacat atttttccat
     3841 ttgcggatga tcatcttccg caaaataagc gggcagcagg ttcgggtgat tcgggaacat
     3901 ctcccacagt agcggtagaa gtgccttgtt ggagataata ctcttccacg ccggttccag
     3961 ccagcgtacg ccagcatctt ccagcttggt ggagaacatt tcgcgcagca taaattccca
     4021 cggataaagc ttgaacaggt tagaaatcac ctgatcctgt aaatccgtga actggccttt
     4081 ttcgcctaaa cctatctcgt cgatgtagag gaactcggtg gcgatttcag cttcggttgc
     4141 gcagtcctgc aaatactgaa tggttccgcg atcttccacc gtgtcgcgac agcaggtgag
     4201 atgcagcaac tggaagccac actgttcacg cagctcaacg aagcgatcga tcagtttttc
     4261 ttgcagactg ttaaactggt cgctgccctc cggcaagtta ccggcgttaa gctgatcttc
     4321 cagccagatc cactgaaaga acgccgcctc gtatagtgac gttggcgtat cggcgttatt
     4381 ttccagaagt ttaggttcgc cagtgccatc ccacgccaga tcaagacgcg aataaagcga
     4441 tggctggtga gtcagccatg attgacgcac aaaactccag gtgtgttttg gtatgcggaa
     4501 tttggtcatc agctcatcgc tggcgatcac tttttccacc actttcaggc acatctggtg
     4561 cagttcggcg gtgacttctt ccagcttttc aacctgggcg agggtcaact tgtagtaagc
     4621 atcttcacac cagtacggct cgccgtacat ggtgtgaaaa ttgaaaccgt attcgtgggc
     4681 tttctcacgc cagtccgggc gctcggtaat actgactctt tccatcggta tcagccaccc
     4741 attgaacgag aagaggtgcc ggttgcgcta cgctgcatag tgctttgttt ggcaacagat
     4801 tcaccaaaac cgccacgggt aacggtagtg gtggtcgccg gttttggtgc cattgccgtc
     4861 ttcggtacgg tcatggtgcg gcctggctgg gctgcgccat agtttttacc cgtcgcgtcg
     4921 gtatatttac cgtaagccgg actggccggg tttttcgagg agaacagcgg ctgctgtgca
     4981 aatcccgcgc cgccgcccat cagacgcccc atcatgtaac cggccatcag cggcatccag
     5041 aaactcccgc tggattgctg agcctgcgcc tggttttctg gtgccatgcc agcctgggct
     5101 ggtgcttgct gacactgacc ttcaccaaat tcagcaacac agtcttcacg ggtggcgtat
     5161 ttcggcgcag tacgttcggc ttctttcagc gcattgttgt acgcggtggt acattcggcg
     5221 cttttgcctg ggtttgcagc tgaacagtcg tcagcatttt gatagagaga cactgtttca
     5281 tcactctttt cacagccagc cagcataaaa acagtggcaa ccgcgagagc gactggtgtc
     5341 agatggcgtg cgctccagtt tttgcggaac gatgcgtggc gtatggattt tgtccgtttc
     5401 atttttgtct tccgggacca gtggtaaata cccatcagaa tagaggatgg ctggtcgaaa
     5461 ttgaagcgag aagggggaag aatgcggcag ataacccgta tctttacgtt gccttacgtt
     5521 cagacggggc cgaagccccg tcgtcgtcat cagttacgga aagggttatg accgttactg
     5581 gtggtagtgc gtgcggatgt ttgctgaacg acgggtgccg ggctatcagg cgcataacca
     5641 tcagcaatag cattctgttc cggcgtttgc ggggcaacgt tttccggatt agtggaaacc
     5701 ggtttgctca gcgcattgtt cagtgccagc aaatcctgct cgttcaacgt acccagggct
     5761 gacttaatat tcagctgatt aatcaggtag ttataacgcg cattcgccag ctcttgcttg
     5821 gcgttgtaca gcgtggtggt cgcatccaac acatcaacaa tggtacgcgt accgaccgag
     5881 tagcccgctt ccatcgcgtc taatgagctt tgcgcggaaa ctacggcttg tttgtaggcg
     5941 ttaatactac tgatagaagc attaatgttg ttgaaggagg aacgtacggt ttgcacgacg
     6001 ctacgatgtg cgctttccag ttgctcactg gcaccaacaa agttgtactg tgcctgtttc
     6061 acctgcgagt taaccattcc gccctgataa atcggcagcg agaagctcag gccaactttg
     6121 ttctggccca tattgctatc gtcatactgg gtaccagcgg caccacgggt tttcgaaccg
     6181 ctataagagg tgtcagaaat cccggtagaa gccgttaaat ccagagtcgg taagtgacca
     6241 tcctgcgcct ggcgaatttg ctcgcgcgcc aggtcctggc tcaagcatgc ctgtaacagc
     6301 gacaggttgc gtttttcggc ttctttcagc agcgcgttaa ccggctgtgg tttgtcggtt
     6361 ttaaagtttt cgacattcag cgccgccagt tccggatagt agttaccggt gatctggcgc
     6421 agctgctcta ccgcgttatc aaggttatta cgtgcggtca cttcgttcgc cagcacggta
     6481 tcgtactgcg cgcgggcgtt ctgcacgtcg gtgatcgcta ccaggcccac gttaaaacgt
     6541 tgggtggttt gatctaattg acggtagatc gcttcttttt gtgcctgtgt ataggaaaga
     6601 acgtcaatag cattcaacac gttgaaataa gcggtcgcgg tgttgaggat caaggtttgc
     6661 tgatcggtct gatatgtgac gtcctgaatc cctgctgctt tttcctgcag cgttaacgca
     6721 cgccatttcg acatatcaaa aatggattga gttaactgca gggacgcact ggtcgcgttc
     6781 gagttgatgc cgttcgcgtc gcggtagccg ttgctatagg tgtaatctgc acctaaacct
     6841 agctgtggca gtaatggact gcgcgcttca ttaatttttt caaaggcagc atcacgatcg
     6901 gcggcagact tacgcaattc cgggttacta aggcgtgctt gctgataaac ttgcatcagg
     6961 ttctcggcct ggctcaacga actgaaccca gaaaggctca ggccgataag aatggggagc
     7021 aatttcttca tttgcattcc ttgtggtgaa gcagtattta gcgcgatcaa actgtaaaat
     7081 tattgcggat tctagcagaa gccgctaccg caaaaggttg gcgttacgtg ccatagggcg
     7141 ttaatgtgcc tcaatttaac atatgactgc tcaaacggca gtcaaacgtc gctgaaattc
     7201 acatttaatt cactattagt gccaggacat taacaatgct taagccagac aacctgcccg
     7261 ttacatttgg caaaaacgat gtagaaatta ttgcacgaga aacactttat cgcggctttt
     7321 tttcattaga tctttataga tttcgtcatc gtctattcaa cgggcaaatg agtcatgagg
     7381 tacggcggga aatttttgag cgcggtcacg ccgcagtctt gctacccttt gacccagtgc
     7441 gtgatgaagt tgtgctgatt gagcagattc ggattgccgc gtacgacacc agcgaaaccc
     7501 cctggctact ggagatggtt gccgggatga ttgaagaggg tgaaagtgtg gaagatgttg
     7561 cccgtcgcga agcgattgaa gaggcgggac tgatagtcaa acggaccaaa ccggtgttaa
     7621 gtttcctggc aagcccgggg ggcaccagtg agcgttcgtc aattatggtg ggcgaagtgg
     7681 acgccacgac cgcaagcggt attcatggtc tggctgatga aaacgaagat attcgcgttc
     7741 atgtggtaag ccgggaacag gcataccagt gggtagaaga ggggaaaatc gacaacgcag
     7801 cgtcggtcat cgctttgcaa tggctgcagc tgcatcatca agcgttaaaa aatgagtggg
     7861 cataaatgaa gcgttacaca cctgactttc ctgaaatgat gcgcctgtgc gagatgaact
     7921 tttcataatt gcgccgtttg ttaccgcgca attacgcacc cggcgaaact gtaagctatc
     7981 aggtggcaaa cgcacaatat cggctgacga ttgtggaatc gacccgatac actaccctgg
     8041 tgacaataga acagactgcg cccgcgatca gttactggag ccttccgtca atgacggtgc
     8101 gtctgtatca tgacgcgatg gtggctgaag tgtgttcaag ccagcagatt tttcgcttca
     8161 aagcgcggta tgattatcct aataaaaagt tgcatcaacg cgacgaaaag catcaaatta
     8221 accagttttt agcggactgg ttgcgatact gtttagcaca tggagcgatg gcgattccgg
     8281 tttactagcg tcgtgaaacc taaggacacc atttggaaag cctgttaacc cttcctctgg
     8341 ctggtgaggc cagaggaagg attttacaaa ttaccgatac tcacctgttt gcacaaaagc
     8401 acgaagccct gttaggggta aacacctggg aaagctacca ggcggtgctg gaggcgattc
     8461 gtccacacca gcacgaattc gacctgattg tcgcgacagg tgatttagcg caggatcaat
     8521 cctctgcggc ctatcagcat ttcgctgaag gcatcgcaag ttttcgtgcg ccctgcgtct
     8581 ggctgccggg caaccacgat ttccagcccg cgatgtacag cgcgttacag gatgcgggta
     8641 tctccccggc gaagcgggtg tttattggtg agcaatggca aatcctgttg ctggatagcc
     8701 aggtgtttgg cgtgccgcac ggtgagctga gcgagtttca gcttgagtgg ctggaacgta
     8761 aactggccga tgcgccagaa cgccatacgt tgctgctgct gcatcatcat ccgctacctg
     8821 cgggttgtag ttggctcgat caacacagtc tgcgtaacgc gggcgaactg gataccgtgc
     8881 tggcgaagtt tccgcacgtc aaatacttgc tgtgcggtca tattcatcag gagctggatc
     8941 tcgactggaa cggtcgccgc ctgctggcaa cgccgtcgac ctgtgtgcag tttaagccgc
     9001 actgttccaa ctttacgctg gatactatcg cgcccggctg gcgtactctc gagttacatg
     9061 ctgatggcac gctgaccacc gaggtgcatc gcctagcgga cacacgtttc caacctgata
     9121 ccgcttcaga aggctactga tgtctacgct tctttattta cacggtttca acagctcgcc
     9181 gcgctctgca aaagcgagct tgttaaaaaa ctggctggcg gaacatcacc ctgacgttga
     9241 gatgatcatt ccgcagttgc cgccgtatcc ttccgacgcg gcagaactgc tggaatccat
     9301 tgtcctggaa catggcggcg attcgctggg tattgtcggt tcgtcactgg ggggatatta
     9361 cgccacctgg ttgtcgcaat gttttatgct gcccgcagtg gtggtaaacc cggcggtacg
     9421 cccgtttgaa ttgctgacgg actatctcgg tcagaacgag aacccctaca ccgggcagca
     9481 atatgtgcta gagtcacgcc atatttacga tctcaaagtc atgcagattg acccgctgga
     9541 agcgccggat ttgatctggc tgctgcaaca gacgggagat gaagtgctgg attaccgcca
     9601 ggcggtggcg tactacgctt cctgccgcca gactgtcata gaaggcggca accacgcatt
     9661 cacgggcttc gaagattatt tcaacccgat cgtcgatttt cttggtctgc accatctctg
     9721 acgatcaact cgaattgcta acttaaacca tgacgcaaac ttataacgct gatgccattg
     9781 aggtactcac cgggcttgag ccggttcgcc gccgtccggg gatgtatacc gataccactc
     9841 gccctaacca tttggggcaa gaagttattg ataacagtgt cgatgaagca ctggcgggtc
     9901 acgcaaaacg cgtggatgta atcttacatg ctgaccagtc gttagaagtg attgacgatg
     9961 ggcgcgggat gccggtggat attcacccgg aagaaggagt accggcggtt gaactgattc
    10021 tttgccgtct gcatgcaggc ggtaaattct ctaacaaaaa ttaccagttc tctggcggcc
    10081 tgcatggcgt ggggatttcg gtagttaacg ccttgtcgaa gcgcgtagaa gttaacgtgc
    10141 gccgcgatgg tcaggtttat aacatcgcct ttgaaaatgg cgaaaaggtg caggatttac
    10201 aggttgtcgg cacttgcggt aaacgcaata ccggtaccag cgtgcacttc tggccggatg
    10261 aaaccttctt tgacagcccg cgtttttctg tttcacgcct gacgcatgtg ctgaaggcca
    10321 aagcagtact gtgccccggc gttgagatta cctttaaaga tgagatcaac aacaccgaac
    10381 agcgctggtg ctatcaggac ggtctgaatg attacctggc ggaagcggta aacggtttac
    10441 cgacgctgcc agaaaaaccg tttatcggta atttcgctgg cgatactgaa gctgtggact
    10501 gggcgctact gtggctgccg gaaggcggtg aactgctgac cgaaagctac gtcaacctta
    10561 tcccaacgat gcagggcggt acccatgtta atggtctgcg tcagggcctg ttggacgcga
    10621 tgcgtgagtt ctgtgaatac cgcaacattc tgccgcgcgg cgtgaagctg tcggcggaag
    10681 atatctggga tcgctgcgcc tatgttctgt cggttaaaat gcaggatccg cagtttgccg
    10741 ggcagaccaa agagcgtctc tcttcgcgtc agtgtgcggc attcgtttcg ggcgtggtga
    10801 aagatgcctt catcctgtgg ctgaaccaga acgttcaggc ggcggagttg ctggcagaga
    10861 tggcgatttc cagcgcccag cgtcgtatgc gtgcggctaa aaaagtggtg cgtaaaaagc
    10921 tgaccagcgg cccggcgctg cctggcaaac tggctgattg taccgcgcag gaccttaacc
    10981 gtaccgaact gttcctcgtg gaaggtgact ccgcaggcgg atctgccaag caggcgcgcg
    11041 atcgtgaata tcaggcgatc atgccgctga aaggtaagat ccttaacacc tgggaagttt
    11101 cttccgacga agtgctggcc tcgcaggaag tgcatgatat ttcggtagcg atcggtatcg
    11161 atcctgacag cgacgatctg agccagcttc gttatggcaa aatctgtatc ctcgcggatg
    11221 cggactctga tggtctgcac attgccacgc tgctctgcgc tttgttcgta aaacacttcc
    11281 gcgcgttggt gaaacacggt cacgtttacg tcgcactgcc accgctctac cgtattgatc
    11341 tcgggaaaga ggtttattac gcgctgacgg aagaagagaa agagggcgta cttgagcaat
    11401 taaaacgcaa gaaaggcaag ccgaacgtcc agcgttttaa aggtctgggg gaaatgaacc
    11461 cgatgcaatt gcgtgaaacc acgcttgatc cgaacactcg ccgtctggtg cagttgacta
    11521 tcgatgatga agacgatcag cgtactgacg cgatgatgga tatgctgctg gcgaagaaac
    11581 gctcggaaga tcgccgcaac tggttgcaag agaaaggcga catggcggag attgaggttt
    11641 aaaggaaaga acattgcccg gtcaggatgg ccgggcaaac aaggcaggat taaatccctg
    11701 gctgcagaat acggatattc atctccagca cgtcgccttt tacggctttg ctgtacgcct
    11761 tcatgtgcgg ggtttgcaga tgcgcttcaa ggtgcgcgat gctttcccac tgctcaatca
    11821 tcacgataga atccggtgcc atagactgga aactcacgcc agcagcgcaa tccaccattg
    11881 gcgcatagcc gtggcaacct tcttctttca gtacggttgg aacgatttta gcaaactgat
    11941 ccaataccgc ctgacggtga tgttgaccag gacgagtacg gatttctgcg attacggtaa
    12001 gcatggttaa ctccttctaa agcctgagct ctagttaacc aaaaatttcc acaagatgct
    12061 tgcgatattc ttcagtatag cggggaacat caggcatttt tatcacgtca ttagcgataa
    12121 atgtcggcag cggttccata ccgaggaatt ggtttgcttt atggaacggc agatacacac
    12181 cgtcaacgcc aacgccgtgg aagaactgat ctttttcggt gaaggcttcc attggtgcgt
    12241 tccaggttag agaaagcata tattttttgc cctgtaccag gccgccggaa ccgtattttt
    12301 tcgacggatc tttgcgggta cgaccatcgc tggcatacag agtcccgtga ccttcggtga
    12361 atacatcatc aatatatttt ttcactgtcc acggcgcgcc catccaccag cctggcatct
    12421 gccagatcac cacatcagcc cagagaaagt tttgtacttc cgctttgaca ttgtagtcgc
    12481 tgtcggcgcg aacgatgcgg acatcatgcc cgaggtcgcg cagtgtgcca tccgcgactt
    12541 cggtcagggt gtcgttcagt tgaccattgg agtgggcgaa ttttttcgcg ccgttgataa
    12601 tcaggatgtt gctcattttt taacctcaag gtgagacgtt tgtcgactat agtacgtcac
    12661 agagcggtac ggtgaaatta gcaaaatgtg caaagtcttt tgcgaattta gcaaaagtct
    12721 gatctagctc tgactcaccg atgcaggata aatattttct gaaaatgaca atactgatag
    12781 ccattcctca taattcttac tcgtaccgga ataatttaca cgtttggttc ggtttttttg
    12841 gggattgaaa atatggcttt gaataagtaa aagatgaaaa tatcaacgtg tcatgttgat
    12901 aattataaat ggatttttat gagcgtactg atatatgtta aagcagaaaa ttaaaacaat
    12961 atttgaagca ttgctatata taatgcttac ttattggtta atagattctt tttttgcttt
    13021 caataagtat gactggatgc ttgagtcggg aggcaatatt tgcagtatac cttccgtttc
    13081 aggcgaagat cgtatattgc aagcaatgat tgcagcattt tttcttctta cgccacttat
    13141 tattcttatc ttaagaaaac tctttatgcg ggaaatgttc gagttttggc tatatgtatt
    13201 ctcgctcgtt atttgtctgg tttgtggttg gtggcttttt tggggacggt ttatattttg
    13261 ctattagtaa aatctatgag agtcgtttta acggctctca tagacagaga agttaccagc
    13321 ttaccttcgc ctcaaatcca ccttgttccg cattcccaaa ctcaacattc atgtcatgca
    13381 gcttggcgat tcgctggaca atcgatagcc caagtccact gccggtagcg gtttgtccgg
    13441 gtgggcgata gaagcgttcg ccaattcgcg ccagtgcctc tggtgtcaca ccggggccgt
    13501 tatccctcac gatgaaatta tcagcattca gcgtgacgtc tatcacgctg ccctgtggac
    13561 tgtagcgcac ggcgttatcc agcaaatttc gcaccaacaa acttagcaat agcggttgtc
    13621 cggtgcgttt gatgccgtgg gcattgagtg tcagtcgcac gtcaattttc gcctgctgcg
    13681 ccgtgtggta aatatccatc accgacgatt gtaggagatc ttcaagcggg atctccgcga
    13741 cgtcctgaag gttatccagt gagtccagcc gcgatagcgt gagcagttga tcaaccagac
    13801 gagtagcgcg atcgatcccg gaatgtaatt ggagcagtgc ttttttccgc gcctgcggat
    13861 catcgtcaga gagctgcgca acttcggttt gcactttcag cgctgttaac gggctacgta
    13921 gttcgtgagc tgcatcggag gtaaagcgtc gttctcgaac catcatcgca tgtgtgcggg
    13981 cgaacagttg atttagcgac tcaaccagtg gccgcacttc gctgggtacg ccagtcgcgt
    14041 ttagtggttt ttccgagtca gggtcacgca tacgtagtgc cagcgccagt ttgttcagcg
    14101 gcgcgagttc acgacccagt agtaccatca tgatgattaa cataatcggc agtgcgacca
    14161 gccacgggat caattgcccg gcaacaatcg ccagcgccat gtcttcacgg tattcccatt
    14221 cctggccaac aacgatgcga tatttgccat caggtgaggt catccagaca aaacgccaag
    14281 gatcgtcttc accgaccagt tgcccgtcag caaaaccttc ccgttgatag ctatagggaa
    14341 tatcttctcc gttatcgcca tcattaagga ccattctgcc gtcgtgggta aagatggcaa
    14401 aggtcagcgc atcgtcatca acatgaccgt gttttaattt atttggcgtc tgtgccatgc
    14461 gatccgccgc gttgatttcg ttgagatcga gcgtacttaa ccgtttggca aacagcatca
    14521 gttgggtgtc gaacaattca tcgacgttat ccgttgtttg tttccaggcg acaaagctgg
    14581 aaagcagcca ggtcaccgag gccagaatta aaaagattag cgtcagcctg acgcgcagac
    14641 taagacgttg ggtaaatttc atttctcacc taatgtgtag ccaataccat gcacggtacg
    14701 aataaaatca ctaccgagtt tgcgtcgcag atgatgcaca tgcacttcaa cggcattact
    14761 ggtgacctct tcgtcccagg tatacagttt ctcttcaatc agtttgcgcg gcagtacccg
    14821 accagcgtta cgcatcagta attccagcag ggcaaattct tttggtttca gtgttaaggg
    14881 ttcgccagcc agcgtggcga tacgtttgcc ggggtcgagc atgacgttac cgtggcgcag
    14941 ctcgttgctg gcctggccgt tggttcggcg catcagagcc tccagcctgg cggcgacttc
    15001 tatcaacgca aaaggtttac acagataatc gtcagctccc agacgcagcc cttctacacg
    15061 ttccgctaac gcatcacgcg cggtcaggtt cagtaccggc tcacgctgac ctttctctcg
    15121 ccattcgcgc aaaatatcgc gaccatccat gcctggtaag gttaaatcca ggatcaccgc
    15181 atcataaggt gcgctataaa gcgcctcttt tccctgacga ccttgtgtaa accagtcgac
    15241 actaaaaccc attttactaa ggccagtttt gatgccgtca ccaatcagca tgtcatcttc
    15301 tatcagtaaa attcgcattt tttcatccct gcgataaccg tatttgctgc cagtaaaccg
    15361 tgttgtaacg cgctctgtac agcattaaat tttatttttt tcccttaaga agttgttaag
    15421 gactatcttg ttaaatgctc ggaaacagac attaaaggga gtaataaaca tgaaaaaatt
    15481 cgcagcagta atcgcagtaa tggccctgtg cagtgcaccg gtgatggcag cagagcaggg
    15541 cggtttttct ggcccatcgg caacgcaaag tcaggccgga ggatttcagg ggccgaacgg
    15601 cagcgtaacg actgtagaaa gcgtaaaatc cctgcgtgac gacacctggg taaccctgcg
    15661 cggcaatatc gttgaacgca tctctgacga tctctacgtg tttaaagatg ccagcggtac
    15721 tatcaatgtt gatatcgatc acaaacgctg gaacggtgtg acggtgacgc cgaaagatac
    15781 ggttgagatt cagggtgaag tcgataaaga ctggaattct gttgaaattg acgtcaaaca
    15841 gatccgcaaa gtaaatccgt aattgttacc gctcccggga cgcgttcccg ggaataattt
    15901 cgcagggagg caaaatgaca aacctgacac tggatgtaaa cattatcgat ttcccatcaa
    15961 tccctgtggc gatgttgccg caccgctgta gccctgagtt gctcaactac agtgtggcga
    16021 aatttatcat gtggcgtaaa gagacggggc tttctcctgt taaccaaagc cagacttttg
    16081 gcgtcgcctg ggacgaccct gccaccaccg caccggaagc gtttcgcttt gatatctgcg
    16141 gcagcgttag cgaaccgatt cccgataatc gttatggtgt gagcaatggt gaacttaccg
    16201 gtggacgtta tgccgtggcc cgccacgttg gcgagctgga cgatatttca cacacgatat
    16261 ggggcattat tcgccactgg ctgcctgcaa gcggcgagaa aatgcgtaaa gcaccgattc
    16321 tgtttcacta caccaatctt gccgaagggg tgacagagca gcgactggaa acggatgttt
    16381 atgtgccgtt ggcgtgatgg ggaatcttta ccggagtgtg agccaaatca ctcgcgataa
    16441 gtttgagcga acaatgtcac aggcgtattg ttgttgggct tataatttct ccggtaagga
    16501 ataccatgta tacgcgaaat ttattatccg gtgcgccgta agccgtctcg ggggtatcca
    16561 tgagcaagcc gcacttatgg caggaggcgc ggggcactgg tggagacaca gctaaactgg
    16621 gctgaaccgt atacttacag tcgactactt tgatgcttta ggcttccctg gactcgcata
    16681 acctcaactt ctcgaatcgc ccggagcgga cccccatgcc gagtggtgtg gcatgggcgc
    16741 aaccttgtgc tgccctctat gccgatcaag tctacagcaa agagttgtat atcaaggcac
    16801 attgatgcct ttatcgccgt cggagcacgt ccggcggcgc tattcacgct gatctcctgt
    16861 gactcgacgc ggcagataat gtagtatctc cggcaatatt gcccctttga aggctggcga
    16921 ataagttgag gaatcagaat taatgagcga tatggcagag cgccttgcgc tacatgaatt
    16981 tacggaaaac gcctacttaa actactccat gtacgtgatc atggaccgtg cgttgccgtt
    17041 tattggtgat ggtctgaaac ctgttcagcg ccgcattgtg tatgcgatgt ctgaactggg
    17101 cctgaatgcc agagccaaat ttaaaaaatc ggcccgtact gtcggtgacg tactgggtaa
    17161 ataccatccg cacggcgata tcgcctgtta tgaagcgatg gtcctgatgg cgcagccgtt
    17221 ctcttaccgt tatccgctgg ttgatggtca ggggaactgg ggcgcgccgg acgatccgaa
    17281 atcgttcgcg gcaatgcgtt acaccgaatc ccggttgtcg aaatattccg agctgctatt
    17341 gagcgagctg gggcagggga cggctgactg ggtgccaaac ttcgacggca ctttgcagga
    17401 gccgaaaatg ctacctgccc gtctgccaaa cattttgctt aacggcacca ccggtattgc
    17461 cgtcggcatg gcgaccgata ttccaccgca taacctgcgt gaagtagctc aggcggcaat
    17521 cgcattaatc gaccagccga aaaccacgct cgatcagctg ctggatatcg tgcaggggcc
    17581 ggattatccg actgaagcgg aaattatcac ttcgcgcgcc gagattcgta aaatctacga
    17641 gaacggacgt ggttcagtgc gtatgcgcgc ggtgtggaag aaagaagatg gcgcggtggt
    17701 tatcagcgca ttaccgcatc aggtttcagg tgcgcgcgta ctggagcaaa ttgctgcgca
    17761 aatgcgcaac aaaaagctgc cgatggttga cgatctgcgc gatgaatctg accacgagaa
    17821 cccgacccgt ctggtgattg tgccgcgttc caaccgcgtg gatatggatc aggtgatgaa
    17881 ccacctcttc gctaccaccg atctggaaaa gagctatcgc attaacctca atatgatcgg
    17941 tctggatggt cgtccggcgg tgaaaaacct gctggaaatc ctctccgaat ggctggtgtt
    18001 ccgtcgcgat accgtgcgcc gccgactgaa ctatcgtctg gagaaagtcc tcaagcgcct
    18061 gcatatcctg gaaggtttgc tggtggcgtt tctcaacatc gacgaagtga ttgagatcat
    18121 tcgtaacgaa gatgaaccga aaccggcgct gatgtcgcgg tttggcctta cggaaaccca
    18181 ggcggaagcg atcctcgaac tgaaactgcg tcatcttgcc aaactggaag agatgaagat
    18241 tcgcggtgag cagagtgagc tggaaaaaga gcgcgaccag ttgcagggca ttttggcttc
    18301 cgagcgtaaa atgaataacc tgctgaagaa agaactgcag gcagacgcgc aagcctacgg
    18361 tgacgatcgt cgttcgccgt tgcaggaacg cgaagaagcg aaagctatga gcgagcacga
    18421 catgctgccg tctgaacctg tcaccattgt gctgtcgcag atgggctggg tacgcagcgc
    18481 taaaggccat gatatcgacg cgccgggcct gaattataaa gcgggtgata gcttcaaagc
    18541 ggcggtgaaa ggtaagagta accaaccggt agtgtttgtt gattccaccg gtcgtagcta
    18601 tgccatcgac ccgattacgc tgccgtcggc gcgtggtcag ggcgaaccac tcaccggcaa
    18661 attaacgttg ccgcctgggg cgaccgttga ccatatgctg atggaaagcg acgatcagaa
    18721 actgctgatg gcttccgatg cgggttacgg tttcgtctgc acctttaacg atctggtggc
    18781 gcgtaaccgt gcgggtaagg ctttgatcac cttaccggaa aatgcccatg ttatgccgcc
    18841 ggtggtgatt gaagatgctt ccgatatgct gctggcaatc actcaggcag gccgtatgtt
    18901 gatgttcccg gtaagcgatc tgccgcagct gtcgaagggc aaaggcaaca agattatcaa
    18961 cattccatcg gcagaagccg cacgtggcga ggatggtctg gcgcaactgt acgttctgcc
    19021 gccgcaaagc acgctgacca ttcatgttgg gaaacgcaaa attaaactgc gtccggaaga
    19081 gctacagaaa gtcactggcg aacgtggacg ccgcggtacg ttgatgcgcg gtttgcagcg
    19141 tatcgatcgt gttgagatcg actctcctcg ccgtgccagc agcggtgata gcgaagagta
    19201 atatgccggg gagggcgctg tttcctcccg ctagcaagga acgccggatg aaacgttaag
    19261 tcgtcatccg gcagcgtatt caacccccgg aaacgataaa aattcccttg aaaccgttgt
    19321 ttattcatgc gttgcgatta acaatacgct tttccagaga gcggctttta acaatgccct
    19381 aacctgattt caggtgacgt acaatgccag tctctcagac cttcagaggg tgttatgcta
    19441 tatatctttc gtcttattat taccgtgatt tacagcatct tagtctgtgt attcggctcc
    19501 atttactgcc ttttcagccc gcgtaacccg aaacatgtgg ccacctttgg gcatatgttt
    19561 ggccgtcttg cgccactgtt cggcctgaaa gttgagtgcc gcaaacctgc agatgctgaa
    19621 agctacggca atgctatcta tatcgctaac caccagaaca actatgacat ggtgacagca
    19681 tcgaacattg tgcaaccgcc gacggtgacg gtaggtaaaa agagcttgct gtggatcccc
    19741 ttcttcgggc agttgtactg gttaaccggc aacttattga tcgacagaaa caatcgcact
    19801 aaagctcacg gcaccattgc ggaagtcgtg aatcatttca aaaaacgccg tatttccatc
    19861 tggatgttcc cggaaggaac ccgcagccgt ggtcgcggcc tgctaccgtt caagactgga
    19921 gcatttcacg cggcaattgc ggcgggcgtc ccgattattc ccgtgtgcgt ctctacaact
    19981 tcgaataaga ttaatcttaa tcgactgcac aacggtctgg tgattgtcga aatgctgccg
    20041 ccaattgacg tcagtcagta tggcaaagat caggttcgtg agctggctgc ccattgtcgt
    20101 tcgataatgg aacaaaaaat cgccgagctc gataaagaag tcgcagaacg cgaagccgcc
    20161 ggaaaagttt aagtcggcaa tctgtatttt tgcggggaac actttcctgc acggtattac
    20221 tttagccagt tttacatgga gcaaatatgt cactcagtcg gcgtcagttc attcaggcat
    20281 cggggattgc actttgtgca ggcgctgttc ccctgaaggc cagcgcagcc gggcaacagc
    20341 aaccgctacc cgttccgccg ctacttgaat ctcgccgtgg gcaaccgctg tttatgactg
    20401 tacaacgtgc gcactggtca tttacgccag ggacacgcgc gtcggtctgg ggaatcaatg
    20461 gtcgttacct ggggccgact atccgcgtct ggaagggcga cgatgttaag cttatttaca
    20521 gcaaccgcct gacagaaaat gtctcaatga cggtggccgg gctacaggta ccaggcccgc
    20581 tgatgggcgg tccggcacgg atgatgtcgc caaacgctga ctgggcaccc gtactgccta
    20641 ttcgccagaa cgcagctact ctgtggtatc acgccaatac tcccaaccgc acggctcagc
    20701 aggtctataa cggccttgcc ggaatgtggc tggtggaaga tgaagtcagc aagtcgctgc
    20761 ctatccccaa ccattatggt gtggatgatt ttccggtcat tatccaggat aaacggctgg
    20821 ataactttgg tacgccagaa tacaacgaac cgggaagcgg cggctttgtt ggtgatacgc
    20881 tgctggttaa cggtgtacaa agcccgtacg ttgaagtctc gcgtggctgg gtgcgcttgc
    20941 gactgctgaa cgcgtcgaac tctcgtcgct atcaactaca gatgagcgat ggtcgcccgt
    21001 tacatgtgat ttctggcgat cagggattcc tgcctgctcc tgtatcggtg aagcaacttt
    21061 cgctggcacc gggcgagcgc cgcgagattc tggtggatat gagcaacggc gatgaagtgt
    21121 cgatcacctg tggcgaagcg gcgagcattg ttgatcgtat tcgcggcttc tttgagccat
    21181 ccagtattct ggtttctacc ttggtgctaa cgctgcgccc aaccggcctt ctgccgctgg
    21241 tcacagacag tcttccgatg cgcttgctgc caactgaaat catggctggt tcgccaattc
    21301 gcagtcgcga tatcagtctg ggtgatgacc cgggtattaa tggacagctg tgggacgtca
    21361 accgtattga tgtcaccgcg cagcaaggaa cgtgggaacg ctggacggta cgcgcggacg
    21421 agccgcaagc gttccatatt gaaggcgtaa tgttccagat ccgtaacgtg aatggcgcga
    21481 tgccgttccc ggaagacaga ggctggaaag ataccgtttg ggttgacgga caagtggagc
    21541 tgcttgttta tttcggtcag ccttcctggg cgcacttccc gttctacttc aacagtcaga
    21601 cgctggaaat ggcggaccgt ggctcgattg ggcaactgtt ggtcaatccg gtaccgtaat
    21661 cttcatactc gcccgaggag ggcgagtatg gaataccttt cgatttctct ttcgttccgg
    21721 cttataatcc ccggcctttg attatttttt tatcatttct ggaagcaaaa tgagctctat
    21781 ctccctgatc caaccggatc gcgacctgtt ctcctggccg cagtactggg ccgcctgttt
    21841 tggaccggca ccgtttttgc cgatgtctcg tgaagagatg gatcaacttg gctgggatag
    21901 ctgcgacatc attttggtta ctggcgacgc gtatgtcgat cacccaagct tcgggatggc
    21961 gatttgcggt cgtatgctgg aagcgcaggg ctttcgcgtc gggatcatcg cccagccgga
    22021 ctggagcagc aaagacgact ttatgcgtct gggtaaaccg aatctgtttt ttggtgttac
    22081 tgctggcaac atggattcga tgatcaaccg ctataccgcc gatcgccgtt tacgtcatga
    22141 tgatgcctac acgccagata atgtcgcggg taagcgaccg gatcgcgcca cgctggttta
    22201 tacccagcgt tgtaaagagg cgtggaaaga tgtgccggtg atcctcggcg gtattgaggc
    22261 tagcctgcgc cgtaccgcgc attatgatta ctggtccgat accgtgcgcc gttccgttct
    22321 ggtagattcg aaagccgaca tgctgatgtt tggtaacggt gaacgtccgc tggtggaagt
    22381 agcgcatcgt ctggcgatgg gcgagccgat tagtgaaatc cgcgatgtgc gtaataccgc
    22441 aattatcgta aaagaggcgc tgcctggctg gagcggtgtg gattccaccc gtcttgatac
    22501 cccagggaaa atcgacccaa tcccgcatcc gtatggcgaa gatttgccgt gtgcggataa
    22561 caaacctgtt gcgccgaaaa agcaggaagc gaaagctgta atcgtgcagc caccgcgcca
    22621 gaaaccgtgg gaaaaaacct acgtattgct gccttctttc gagaaagtga agggcgataa
    22681 agtgctgtac gcccatgctt cgcgcattct gcaccacgaa accaacccag gctgcgcccg
    22741 cgcattgatg caaaaacacg gtgaccgcta tgtgtggatc aacccgcctg ctatcccgct
    22801 ttctaccgaa gagatggact gcgtttttgc gctgccgtac aagcgcgtgc cacatccggc
    22861 ctatggcaat gcccgtattc cggcttacga aatgatccgt ttttcggtca acattatgcg
    22921 cggctgcttt ggcggctgtt ctttctgttc tattaccgag cacgaagggc gcattattca
    22981 gagccgttcg gaagattcga tcattaatga gatcgaagcg atccgcgaca ccgttccagg
    23041 ttttacgggc gtgatttccg atctcggtgg gccaactgcc aacatgtata tgttgcgctg
    23101 caaatcgcca cgcgctgaac aaacttgtcg ccgtttgtcg tgcgtttatc cggatatttg
    23161 tccgcacatg gacactaacc acgaaccgac gatcaacctc tatcgccgtg cgcgtgatct
    23221 gaaaggcatt aaaaaaatcc ttatcgcctc aggcgtacgt tatgacatcg ccgtggaaga
    23281 tccgcgctat atcaaagagt tggcgaccca tcacgtcggc ggttatctga agattgctcc
    23341 ggaacatacc gaagaagggc cgttgtcgaa gatgatgaag ccgggcatgg gcagctatga
    23401 ccgctttaaa gagctgttcg atacctactc gaaacaggca ggtaaagagc agtatctgat
    23461 cccgtatttc atctctgcgc accccggtac gcgtgatgaa gatatggtga atttggcgct
    23521 gtggctgaaa aagcatcgtt tccgtctcga ccaggtacag aacttctacc catcgccgct
    23581 ggcgaactca accaccatgt attacaccgg gaaaaaaccg ctggcgaaga ttggttataa
    23641 gagcgaagac gtcttcgtac cgaagggcga caaacagcgt cgtttgcata aagcgttgtt
    23701 gcgttaccac gatccggcaa actggccgtt aatccgccag gcgctggaag cgatgggcaa
    23761 aaagcatctg attggcagcc cgcgattgct tagtgcctgc gccaaccatt gaagagatgc
    23821 gtgaagctcg tcgccagaac cgcaataccc gtccggcgtt gactaaacat acgccgatgg
    23881 cgactcagcg tcagacgcct gctacggcaa aaaaagcgtc gtctacgcaa tctcgtccgg
    23941 tgaatgctgg tgcgaagaaa cgccctaaag cggcggttgg acgttaaaaa aaatccccgg
    24001 caactgacat gctgccgggg atttttttat cattctgcgt acatacgcaa gcagcgcagg
    24061 caatcaaaat agtcctgtgc atcaagacgc gtattggctc tgttagcttc ggatttggca
    24121 gcatagattt gtgggcgtag tgctttcata tcctgctcaa gccgggcgat tttggcattt
    24181 aatgtttgta cattgctcgc cagttgattg accttttgct cattgacgca gccactgagc
    24241 aagcaggttg ccagaacaac agctcccacg gtgaaaatcg ttttcatata gtgttccctt
    24301 ttatttattg ttccggctaa ttgttattta attcagctca ttgctttaat ctggcggcgg
    24361 tttttgcctc aatcagctta agtaggctgc cgctgttcgg gcggataaaa tcagcgattt
    24421 cccacttacc ttcgcgccgc tgtaaaacca gctcttgctc ttcattgttg ccattggtca
    24481 gcgtaaaacg aacatgaatg cgggcggaat cggcggtttt ctccaggact cgaatttcat
    24541 caacgcgtga aaatagcgac tcttcgccaa aacagatcgg attgtcgtaa ataaattgcc
    24601 gttctatttg catatcttca tcgggattat tggccgtctt gaaggtggtt ttatagaagg
    24661 cattaaccgc ctggcggtaa gagtcggaaa ccacccacgt ccacggccag ctataatcag
    24721 caatatgttc aaagagttca gcaacaaagg attctggctg ttctttctgc aacgaagcta
    24781 aagcggccag cgttttttca ttctcgctat taactgcttg taagacgctg ccatgattgc
    24841 tgacaatatc atcaatgacc caacgaccat tttccgccac cattttcagt gtctgtgtgg
    24901 tcttttcttt atcatcttta aagatacgaa agcgcacaac ggcatcggca tgatcgacgt
    24961 cagtttgggt tatcgctacg ctttctaaca ccagatcgcc aaaatcctga caatcacaaa
    25021 ccggatcata atccagccag ccaatattgc ccggtagtgt aagattgttg ttcagggtaa
    25081 gcgcctgttg aatacgcgct gaagttatcg cccgctctcc ggtttcaccg aaataaggcg
    25141 tactggcatc tgatttatag ttctgataaa tctgccggac tgtttgttct acggtctgtg
    25201 aaggaggctg cgcgtgtacg gtaaaacttg ccagggctgc taaaaacaga agtattattt
    25261 tcatctgttt ttcacctctc cgttgcgttt atcccggata tagatgagtt gttcttttaa
    25321 gagcttccgg ctctgcatga tgatgtcctt atatttggca ttcctgaaca aggaagagta
    25381 acaacgggcg ggacgcgagg ggaataaatg atttctgaaa agtccggtag cggaacatta
    25441 ccgccaccgg gagaatttgc acgttagccg ccgaactggt caggatcggg accgagacgc
    25501 ttgccctgat cgagttttgc aattttgccg agttcgtctt tgtcgagacg gaaatcccag
    25561 acatcaaagt tttcggcaat acgtgaaggt gtgaccgatt tcgggatcac caccaggccg
    25621 ctatccagat gccagcggat aacaatctgc gccggggttt tgccgtattt atctgccaga
    25681 tcgcgaatga ctttctgatc gaaaacgcct tttcctcctt gcgctaatgg gctccaggat
    25741 tcggtctgga ttttgtgtgt cgcgttccag gcgtgtagct ggcgttgttg catcagcgga
    25801 tgaagttcga tctggtttat cacaggcgtc acgccagttt catcaatcag gcgttgcagg
    25861 tgatggatct ggaagttgca cacgccgatg cttttgatta atccctcttt ttgcaattcg
    25921 atcatgcctt tccatgcttc gacataatgg tcgatagcgg gaacgggcca gtgcattaag
    25981 tagaggtcga tataatcaag ctggagtttt ttcaggctgt cgagcagggc ttcgcggggg
    26041 cacttgtggt cgtcgttcca cagcttagtg gtgatgaaca gttcttctct gttgactgag
    26101 gcatttttca gggctttgcc gacaccttct tcgttcttgt aggccgcggc ggtatcaatc
    26161 gagcgataac ccacttctaa cgctttttga atggcggtga ttacttcctc attacttgct
    26221 tgccagacgc ccagtcccag ctggggcatg acattgccat cctgtagctt aataacggtt
    26281 ggattagcca tacgttcctc ctttatatga actcaccgga gcaggctccg gtgaggtgtt
    26341 gtgaacttaa gtctggacga aatgcccgaa aacgaaagtt tgaggcgtaa aaagcttagc
    26401 gggcggcttt gtatatacgg cggctgacat ccaacgtaat gtcatgattt tcgcccagtt
    26461 gggtcatgcc gtgctcttcc agttttttca gcaaagccgg gatggagctg ccgtccagac
    26521 cgtagtcgga gaggtgggtc ggcacgccta attgctcaaa gaaattgcgg gttgcggcaa
    26581 tcgcggcgtc aatacgctca tcgtcggaac cttcagtgat gttccagacg cgttcagcat
    26641 attgcagcag cttagcgcgc ttggtctcgc gtttttcatt ccacagtgca ggcaggacga
    26701 tagccagtgt ttgcgcgtga tccagaccgt gcatcgcagt cagttcgtgg cccagcatat
    26761 gcgttgccca gtcctgcggt acgccagcgc caatcaaacc gttcagcgcc tgagtcgccg
    26821 cccacatgac gttggcgcgc acatcgtagt tttctggctc tttcagggct ttcggaccat
    26881 cttcgattag cgtcagcaaa atgccttctg cgaaacggtc ctgaattttg gcatcaaccg
    26941 gtttggtaac atactgttcc acggtgtgta caaaggcgtc cactacgccg ttagccacct
    27001 gacgcggcgg cagggtgtag gtataaaccg gatcgagcac ggcaaatacc ggctgaacat
    27061 gtgcagaatg gaacgcctgc ttgtcgcctg tggttttacg ggagatcacc gcgcctgcgt
    27121 tggattctga accggttgct ggcagcgtca gcacacagcc catcgggatg gcgcttttaa
    27181 tctctttacc gcccgtttgc agaatgtgcc acggatcgat attttccgga tagttagccg
    27241 ctgcggcgat aaatttggtg ccgtccagta cagaaccgcc gccaaccgcc agcaggaaag
    27301 tcactttctg ttcgcgaacc agtttcacgg cgttcatcag cgtttcataa gccgggtttg
    27361 gctcaatacc gccaaattcc agcacgtcca tgcctttcag ggcatccaga acttgatcga
    27421 gaacgccggt ttttttcacg ctgccgccac cgtaggtaat caatacgcga gcatcgtgag
    27481 gaatttgttc gcgtaaacca gcgattgcgc ctttaccaaa cagaatgcgg gttggggtgt
    27541 gcagattaaa gttgttcatt acttgctccc tttgctgggc caatatgagg gcagagaacg
    27601 atctgcctga tgtttttcat tgtgatcgcc agcgccctag ctctcaatgc tcatttctgc
    27661 caatgtcttg cctatttctc cagagtgctg gagaaatgct acaaaattgc gcacaatcaa
    27721 attgccgcat tattcctaag aaattacgcg atatgaaacg tgaagagatt tgtcgcttgc
    27781 tggcggataa agttaataaa ctgaaaaata aagaaaatag tttgtcagaa ctgttgcccg
    27841 atgtgcgttt gttgtatggc gagacgcctt tcgcacgtac accggtgatg tacgagcctg
    27901 gcatcataat tctcttttcc gggcataaaa tcggttatat caat
//
LOCUS       DAJTNM010000040        27728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTNM010000040 DAJTNM010000000
VERSION     DAJTNM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.040"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..1090)
                     /gene="ycjM"
                     /locus_tag="OO172_001861"
     CDS             complement(<1..1090)
                     /gene="ycjM"
                     /locus_tag="OO172_001861"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5512067.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQ"
     gene            complement(1302..1649)
                     /gene="pspE"
                     /locus_tag="OO172_001862"
     CDS             complement(1302..1649)
                     /gene="pspE"
                     /locus_tag="OO172_001862"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5512068.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1692..1913)
                     /gene="pspD"
                     /locus_tag="OO172_001863"
     CDS             complement(1692..1913)
                     /gene="pspD"
                     /locus_tag="OO172_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5512069.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1922..2281)
                     /gene="pspC"
                     /locus_tag="OO172_001864"
     CDS             complement(1922..2281)
                     /gene="pspC"
                     /locus_tag="OO172_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5512070.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2281..2505)
                     /gene="pspB"
                     /locus_tag="OO172_001865"
     CDS             complement(2281..2505)
                     /gene="pspB"
                     /locus_tag="OO172_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5512071.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2559..3227)
                     /gene="pspA"
                     /locus_tag="OO172_001866"
     CDS             complement(2559..3227)
                     /gene="pspA"
                     /locus_tag="OO172_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5512072.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3394..4371
                     /gene="pspF"
                     /locus_tag="OO172_001867"
     CDS             3394..4371
                     /gene="pspF"
                     /locus_tag="OO172_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5512073.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCATLNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4491..5756)
                     /gene="puuE"
                     /locus_tag="OO172_001868"
     CDS             complement(4491..5756)
                     /gene="puuE"
                     /locus_tag="OO172_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5512074.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHSDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5794..7074)
                     /gene="puuB"
                     /locus_tag="OO172_001869"
     CDS             complement(5794..7074)
                     /gene="puuB"
                     /locus_tag="OO172_001869"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5512075.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7076..8564)
                     /gene="puuC"
                     /locus_tag="OO172_001870"
                     /pseudo
     CDS             complement(7076..8564)
                     /gene="puuC"
                     /locus_tag="OO172_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(8714..9271)
                     /gene="puuR"
                     /locus_tag="OO172_001871"
     CDS             complement(8714..9271)
                     /gene="puuR"
                     /locus_tag="OO172_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5512076.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNVN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9298..10062)
                     /gene="puuD"
                     /locus_tag="OO172_001872"
     CDS             complement(9298..10062)
                     /gene="puuD"
                     /locus_tag="OO172_001872"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5512077.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10274..11692
                     /gene="puuA"
                     /locus_tag="OO172_001873"
     CDS             10274..11692
                     /gene="puuA"
                     /locus_tag="OO172_001873"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5512078.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11841..12005
                     /locus_tag="OO172_001874"
     CDS             11841..12005
                     /locus_tag="OO172_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512079.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11995..13380
                     /gene="puuP"
                     /locus_tag="OO172_001875"
     CDS             11995..13380
                     /gene="puuP"
                     /locus_tag="OO172_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5512080.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13514..13759
                     /locus_tag="OO172_001876"
     CDS             13514..13759
                     /locus_tag="OO172_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5512081.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14072..15715
                     /gene="sapA"
                     /locus_tag="OO172_001877"
     CDS             14072..15715
                     /gene="sapA"
                     /locus_tag="OO172_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5512082.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVDVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15712..16677
                     /gene="sapB"
                     /locus_tag="OO172_001878"
     CDS             15712..16677
                     /gene="sapB"
                     /locus_tag="OO172_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5512083.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16664..17554
                     /gene="sapC"
                     /locus_tag="OO172_001879"
     CDS             16664..17554
                     /gene="sapC"
                     /locus_tag="OO172_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5512084.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17554..18546
                     /gene="sapD"
                     /locus_tag="OO172_001880"
     CDS             17554..18546
                     /gene="sapD"
                     /locus_tag="OO172_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5512085.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18548..19354
                     /gene="sapF"
                     /locus_tag="OO172_001881"
     CDS             18548..19354
                     /gene="sapF"
                     /locus_tag="OO172_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5512086.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19422..19775
                     /locus_tag="OO172_001882"
     CDS             19422..19775
                     /locus_tag="OO172_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5512087.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20143..20931
                     /gene="fabI"
                     /locus_tag="OO172_001883"
     CDS             20143..20931
                     /gene="fabI"
                     /locus_tag="OO172_001883"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5512088.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21077..22204
                     /locus_tag="OO172_001884"
     CDS             21077..22204
                     /locus_tag="OO172_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5512089.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRENNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22272..24206
                     /locus_tag="OO172_001885"
     CDS             22272..24206
                     /locus_tag="OO172_001885"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5512090.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24284..24386)
                     /locus_tag="OO172_001886"
                     /pseudo
     CDS             complement(24284..24386)
                     /locus_tag="OO172_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24440..26414
                     /gene="pdeR"
                     /locus_tag="OO172_001887"
                     /pseudo
     CDS             24440..26414
                     /gene="pdeR"
                     /locus_tag="OO172_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26562..26735
                     /locus_tag="OO172_001888"
     CDS             26562..26735
                     /locus_tag="OO172_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512091.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26825..27574
                     /locus_tag="OO172_001889"
     CDS             26825..27574
                     /locus_tag="OO172_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5512092.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         6430 a         7191 c         7123 g         6984 t
ORIGIN      
        1 cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag ccacgcagtg
       61 ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt ttactggagg
      121 gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt tgcaccgcat
      181 gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca tcaccgttgc
      241 caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaatgatc actgtacctg
      301 gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt ttttccagat
      361 ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg cggacatact
      421 cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac acttcagggc
      481 tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg cgcgtggaat
      541 tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggacgggtg acggcactga
      601 gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc tgttgtaaat
      661 agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca aacattaaat
      721 gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg gcgacctgat
      781 gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt aataaatgaa
      841 catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg ggtagtggtt
      901 ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca ctttcatccc
      961 agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc gttacaagtt
     1021 tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccaggtaa tccgtaattt
     1081 tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact actccgcaga
     1141 aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt ttttaccagg
     1201 gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata aggtgtgaca
     1261 gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt gaccttcggc
     1321 attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat ctcgctaagg
     1381 atctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac ggtgtcgttt
     1441 ttatccggaa ctgcggtggc aatgcgctct ttcacttcct tcaggggaat attgatggcc
     1501 ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca gtgttcagcg
     1561 gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt taaacatgta
     1621 agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta acaatttttg
     1681 cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg acttaacagc
     1741 ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga ttttatcgcc
     1801 cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag cttgcctgct
     1861 aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt cataactttc
     1921 ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca taacgttcca
     1981 tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc agcaattcgc
     2041 tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca agcgcaaatg
     2101 acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaagaaaatc gacagcacca
     2161 ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg gcgcagacgc
     2221 cgcggaccat gccctgctgt ggaatacgcc ataatttttt attgagatta atgcccgcca
     2281 ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg cctgaatacg
     2341 ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct cactttgcga
     2401 caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg gtaaaacaaa
     2461 cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta ctccttagac
     2521 gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc ttgcttcatt
     2581 ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag ttcggcaaac
     2641 tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc ttccatctgg
     2701 tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt gccactgtcc
     2761 agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat caatgcctgc
     2821 tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt cttcatgcgt
     2881 gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag atcggtcagt
     2941 ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag cagcgccagt
     3001 tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg gcgagtcagc
     3061 tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag tgtatcttcc
     3121 atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc taacagagcg
     3181 ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat gttgtcctct
     3241 tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt gccaattttt
     3301 tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc aggatgaatt
     3361 agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga taatttactt
     3421 ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc accgctggac
     3481 aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc cagccgcctg
     3541 cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc gacgttaaat
     3601 gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac cggtgcgcaa
     3661 aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct tgatgaactc
     3721 gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta cggtgaactg
     3781 gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg cgcgacgaat
     3841 gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct cgaccggctg
     3901 gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat tatgctgatg
     3961 gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt cccggggttt
     4021 acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat tcgtgaattg
     4081 aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc gcttgatgac
     4141 atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt ttcagaaaat
     4201 acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca ggaaaaagag
     4261 ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc tgaattactg
     4321 gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta gcgaagttga
     4381 gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa tcaatacgtc
     4441 gtttgcaggc ctaataagcg cagcgcatca gacattattg cgttgggcta ttaatcgctc
     4501 agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat ggtcagcgga
     4561 tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag cccctgcgcc
     4621 agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt ttgcggatcg
     4681 ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc cggaacgctt
     4741 tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc gcgttcgcag
     4801 agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc cagcgggttc
     4861 ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt cgcgttaccg
     4921 accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat taagtccggc
     4981 ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc gctttgcact
     5041 tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc ggcaaccagc
     5101 tcttttggcg caacgttgaa accgccctcg ccctgcaccg gttcgaaaat aatggccgcg
     5161 acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag ggagtcctgt
     5221 gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc agggaacggg
     5281 ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat atatgtacga
     5341 ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc gcgagcaatt
     5401 ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt agcctgcccg
     5461 ctcactgggg caagggcgtt aattttctcc gccagcgtga cgtagctttc gtacggcaca
     5521 atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc gaccagatca
     5581 gagtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat gtattcgttg
     5641 ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt acacatcacg
     5701 ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt gctcatgttt
     5761 cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat cgcgcaggct
     5821 gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc cggggaacgg
     5881 gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca gcagttcagc
     5941 aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac cctgcatgta
     6001 atagatgttg gtatcaaggc ggccgaactg cggcatgcgc gacaaggtca gcaggaagtt
     6061 gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt ttagtagttt
     6121 cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta cacccccgcc
     6181 gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt tacagtcttc
     6241 cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc gttcggtggt
     6301 gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta ctttatcgcc
     6361 cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat tggcagtccg
     6421 aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat acacgcgccc
     6481 gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt gaatatgccc
     6541 accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc ggcgaatagc
     6601 gttggcgtcc agcagctcca gctgtttatt gccgtagcgt tcccagttct ctttctgctc
     6661 ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg ggcggtagtc
     6721 gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct cgaacatcat
     6781 gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt cgatgtcgcg
     6841 gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc cgatgcgtga
     6901 ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg aggagagccc
     6961 ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca gtgtatcgaa
     7021 tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg tcatttcagg
     7081 cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca tgcagggatt
     7141 tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata tcgccgtcgt
     7201 tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg tgcgcgcggg
     7261 agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg gcaagctgca
     7321 gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa atctcttcgc
     7381 ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg gccgctgcca
     7441 gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca cgaataaagg
     7501 tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt gcgggatcaa
     7561 gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag aattcatcgg
     7621 cgatgctctc ttccagtaac aaacgcgttc cggcgatgca cacctggccc tggttgtaga
     7681 agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg gcgaaaacga
     7741 tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta tcacccgcgt
     7801 ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca tcgatatcgt
     7861 tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg ttcaacacac
     7921 catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg agcggtgatt
     7981 tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg agtttccagc
     8041 aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg accggttcac
     8101 gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac actttgtcga
     8161 tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca cgcagactgt
     8221 gacgaatccg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc ggcgtgggct
     8281 tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga agagagtgac
     8341 cagtcgccgc gttcaaatac gccgcgagct gcgctcaccg cacggtcgat atcgacgctc
     8401 ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt ttcaaaggtt
     8461 tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc aatggcgagg
     8521 cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac tcctgtttca
     8581 cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg ggctgcatgc
     8641 ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt acccggctta
     8701 cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg caaatacctg
     8761 ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt tgccccgcga
     8821 cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt actgtgccta
     8881 tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg tacgtttcaa
     8941 agatcatcgc cagcgtgcga ttcgggttaa cgttatgaac cagcttcatt gacaccccct
     9001 gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca tcaggttttt
     9061 ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg
     9121 tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc
     9181 cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc
     9241 gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca
     9301 gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct cgaacaatat
     9361 acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa
     9421 aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg
     9481 caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc aaaagttgct
     9541 acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt catgcgacgg
     9601 tgcatattgc tgttccaccg gcagttcggg atcttcccga tgctccagca attcaggctg
     9661 ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt gtaaaccccg
     9721 gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg ccatgctcag
     9781 aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg
     9841 cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa gttgttcaag
     9901 taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg cgtggatgat
     9961 ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc tgttcctgca
    10021 cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc tcacaaagtg
    10081 gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat tttttcaatc
    10141 tagcagtgga ttttcatttt tgcaaaccaa atttaacatt tgacaaacat ttggtttgca
    10201 tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga aaacaaacca
    10261 aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga
    10321 agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa
    10381 tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg gaaaacgtat
    10441 ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct cggtatttgc
    10501 aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa tgggcgagcc
    10561 ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg atccagagtt
    10621 tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc
    10681 gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt tcccggttgt
    10741 agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag gggatctgca
    10801 accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt actccgttga
    10861 taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc agttgcagct
    10921 gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa tcaaccttta
    10981 ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac gcctggtgcg
    11041 tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca
    11101 cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg agaatgtgct
    11161 ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg ggatgattga
    11221 cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc gccgcttcca
    11281 gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct
    11341 gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg cgggtgccga
    11401 tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg gccttgataa
    11461 cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag gcttaccctt
    11521 cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc tgcgccgcta
    11581 tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt
    11641 tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt aattcccctt
    11701 gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca
    11761 tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac
    11821 tgtgacaagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggaggctgcg
    11881 tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg ctaaacaggt
    11941 ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc aattatggct
    12001 attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg
    12061 aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac cgtatttgat
    12121 acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta tttgctggcg
    12181 ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggag
    12241 gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg atttatggtc
    12301 ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt gttggcgaaa
    12361 atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc
    12421 gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt caataccctg
    12481 tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt tcagggactg
    12541 cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag cgagaacgcg
    12601 cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct cggtttcgat
    12661 gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc
    12721 ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt tatgcagctg
    12781 ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga aattgcgctg
    12841 tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt gaacacatta
    12901 gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac
    12961 aatgtgtttc cagagcgcgt gtttggctat gtgcacccaa aatggcggac tccggcactc
    13021 aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt agtcaccgcg
    13081 acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt
    13141 aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt ccactatttg
    13201 ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct agagtcaacg
    13261 tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg gtacctgatc
    13321 cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag cgaaacgtaa
    13381 tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca ttaactataa
    13441 aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc
    13501 ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg ccgatgagta
    13561 cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg cccactactt
    13621 ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag cccaacatga
    13681 aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa
    13741 tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg ttgtccggat
    13801 aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct ttaatgggtt
    13861 tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga
    13921 aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac caaccacacg
    13981 gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag
    14041 atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt ctcttttggt
    14101 gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga
    14161 tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc
    14221 caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct
    14281 ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct gggaagtact
    14341 cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga
    14401 ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat
    14461 ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct acttcgacag
    14521 cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata ccgttgagtt
    14581 ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt atgcttcggt
    14641 catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc aactcgaccg
    14701 tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc aatttattcg
    14761 cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg tagtggtgga
    14821 tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat gcgacgttct
    14881 ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct tgcgtttaac
    14941 gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac cgccgctaaa
    15001 taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc tgatgcaatc
    15061 catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct cgtgggccta
    15121 tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac agttgaaggc
    15181 gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa
    15241 ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg ttgacgtaaa
    15301 agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg atatgagcca
    15361 tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt tcttccgtcc
    15421 gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt gcgatccgaa
    15481 attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc gtattgaagc
    15541 ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc tggcgtcgtc
    15601 attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc cgtttggtaa
    15661 cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac catgattatc
    15721 ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc
    15781 ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc
    15841 tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag cattaacggt
    15901 caaccaattg ccgaacagtt aaaagaggtc ttcccggcga cgatagaact gtgcatcctc
    15961 gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg gattacgcgc
    16021 cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc aatcccggtg
    16081 ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg gttgcccgtt
    16141 tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc gttgattgat
    16201 gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat ccgccatatg
    16261 atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt
    16321 atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac ccgcggtttg
    16381 tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc ggttattcct
    16441 cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga aatggttttt
    16501 agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga ttacgcagcc
    16561 atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat
    16621 attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt
    16681 atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga
    16741 cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt gtatttttgg
    16801 cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat tattgccgcc
    16861 atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc tcggacgtga
    16921 tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac
    16981 gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga cacacggcct
    17041 ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt cgctgctgtt
    17101 ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg
    17161 gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg acgaactgga
    17221 aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata ttctctggtt
    17281 tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat
    17341 ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc
    17401 tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt
    17461 catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc ttggcgatgg
    17521 cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg
    17581 accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt aagtatgacc
    17641 ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt
    17701 gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga ccgtatgcgt
    17761 tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat
    17821 aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt
    17881 cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg gcagcgtttt
    17941 ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa agatcacaaa
    18001 gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa agtgatgata
    18061 gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac aaactcaatg
    18121 gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca aaacagcaat
    18181 accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt
    18241 aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt ggtgacgatg
    18301 ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg
    18361 ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga acagttaccg
    18421 attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat tgtgacgcca
    18481 cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa catggagaaa
    18541 gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg
    18601 gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct ttacgctacg
    18661 tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca cactggcaaa
    18721 aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg atcatccgct
    18781 gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc aggatccttc
    18841 gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac tgcgcctgaa
    18901 caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc gtatggttgg
    18961 gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac aaaaacagcg
    19021 tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg atgaagccct
    19081 cgcttcactg gatatgtcga tgcgttcgca gttgattaat ctgatgctgg agttacagga
    19141 aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag
    19201 cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca ccgcggatgt
    19261 gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact ttggcgaagc
    19321 attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct tccagattat
    19381 tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat aaaataaaat
    19441 caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc tggcgatatc
    19501 ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg gggagctaca
    19561 ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt gggcagcatg
    19621 atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag ggctggaccg
    19681 tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg gagattattc
    19741 tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc gaccgagccg
    19801 aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca aggtctgaac
    19861 catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt tgctggggta
    19921 acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg gtaaggggaa
    19981 aagcgccccc cctgccgcag cctgctccgg tcggacctgg caactatagc tactcacagc
    20041 caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga cgaatcgcct
    20101 gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt tctttccggt
    20161 aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg tatcgctcag
    20221 gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa actgaaaggc
    20281 cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg cgatgttgca
    20341 gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc gaaatttgac
    20401 ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga ctatgttaac
    20461 gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag cttcgttgca
    20521 atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac cctttcctac
    20581 cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa agcgtctctg
    20641 gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc
    20701 atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt ccgcaaaatg
    20761 ctggctcatt gcgaagccgt taccccgatt cgccgtaccg ttactattga agatgtgggt
    20821 aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga agtagtccac
    20881 gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata atcgttctgt
    20941 tggtaaagat gggcggcgtt ctgccgcccg cgttatctct gttatacctt tctgatattt
    21001 gttatcgccg atccgtcttt ctccccttcc cgccttgcgt caggataacg atttccttta
    21061 cgaccaagga gcgcccatgg aacaacgcca catcaccggc aaaagccact ggtatcatga
    21121 aacgcaatcc agtactacgg agtatgacgt tctgcctctg gtcccggaag ccgcaaaggt
    21181 cagcgatccc tttctgctcg acgtgatcct tgatgaagag acgctggccc ccttcctttc
    21241 atggctggtc cctgcgcgcg ttcttgcagt ggagttgttc cctgaccagc ttaccgtgac
    21301 ccgttcacaa accttcaccg cttatgaacg cttgtcgacg gccctgacgg ttgctcaggt
    21361 ttgcggcgtc cagcggttat gtaactacta ttcggcgcga cttacgccgc tccccggacc
    21421 tgattcctcc agggaaaata atcatcggtt ggcacaaatc acgcaatatg cccgccaact
    21481 ggctagctcg cctgctatta tcgacaaccg atcgcgccag catctgaatg acgtcggtct
    21541 tactgtctgg gactgtgtga tcattaacca aatcattggt tttattggct ttcaggcgcg
    21601 gacaattgcg acatttcagg cttatctcgg gcatccggta cgctggttac ccgggctggc
    21661 gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa tcaatacgct ggcgaagcag
    21721 ctatgaggtg gaaaaactac ctgaagagca cacaaaaagt tcaactgcag aactttgcca
    21781 actggccgaa acacactctc tccaccctat ttcactttcc cttctcgaaa agttgttaaa
    21841 cagcacacgg gtcaatacac agccggataa tcagcttgcg gcgttgttat gcgcgcgtat
    21901 aaatggcagt cctgcttgtt tttccacctg tatggattca tcaaatgaat ataaaaaaat
    21961 cagcaccctt ctgcgcaagg gcgaaaatga aattaaccga tgggctgacc gtcattctgt
    22021 tgagcgcgct accgttcagg cgatacaatg gctgacccga gcacccgatc gctttagcgc
    22081 cgcccagttc agccctttac tcgaacacga aaaatcatca acgcagatta ttaatctgct
    22141 ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg gtgagacata
    22201 ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca gccgctctaa tggccaccaa
    22261 aatagacaat tatgtttcag gacaacccgc tgctagcgca gcttaaacag caactgcatt
    22321 cccagacgcc acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt ggcttcctgg
    22381 aagtcgacgc acaaaaaagt tatttcattc cgccgccgca gatgaaaaaa gtcatgcatg
    22441 gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg tgaatccgca gagccagaag
    22501 aactggttga accgttcctg actcgtttcg tgggtaaagt tcagggcaaa aatgaccgtc
    22561 tgaccatcgt tcctgatcat ccacttttaa aagacgccat tccttgccgc gcagcccgtg
    22621 gcctgaacca cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc cgtcatccgc
    22681 tgaaaggcga tcgttctttc tatgctgaac tgacacaata catcactttt ggcgacgacc
    22741 actttgtacc gtggtgggtt acccttgcgc gccataatct ggaaaaagaa gcaccagacg
    22801 gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga agatctgacc tcgctggatt
    22861 ttgtcaccat cgacagtgcc agcactgaag atatggatga cgcccttttc gctaaggcgc
    22921 tgccggatga caaacttcag ctgattgtgg cgattgccga tccaaccgcg tggattgctg
    22981 aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat ctgcctggct
    23041 tcaacatccc tatgctgcct cgcgagcttt ctgacgatct ctgctcactg cgcgccaatg
    23101 aagtccgccc ggtactggca tgccgcatga cgctctccgc tgatggcacc attgaagata
    23161 atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat gaccaggttt
    23221 ctgactggct ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt gccgaacaag
    23281 tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac cacgcactgg
    23341 tgtttaaaga tcgcccggat taccgcttta ttctcggtga aaaaggtgaa gtgctggata
    23401 tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg attgccgcta
    23461 acatttgtgc ggcccgcgta ctgcgcgata agctcggttt tggcatctat aacgtgcata
    23521 tgggctttga tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg cacggtctgc
    23581 atgtcgatgc cgaagaagta ctcacgctgg acggtttctg caaactgcgt cgtgaactgg
    23641 atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg cttccagtca tttgctgaaa
    23701 ttagcactga acccggtcct cactttggcc tcggtctgga agcatacgcc acctggactt
    23761 cgccgatccg taaatatggc gacatgatca accaccgtct gctgaaagcg gttatcaaag
    23821 gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca aatggccgaa cgtcgccgtc
    23881 tcaaccggat ggcagaacgt gatgttggcg actggttata cgcacgcttc ctgaaagata
    23941 aagccgggac cggcacccgt ttcgcggcgg aaattgtcga tatcagccgt ggcggcatgc
    24001 gtgtgcgttt ggttgataac ggcgctatcg cctttattcc ggcacctttc ttacacgctg
    24061 tgcgcgatga actggtttgc agccaggaaa acggcactgt acaaattaaa ggtgaaacgg
    24121 tttacaaagt aactgacgtt attgacgtca ccattgccga agtccgcatg gaaacccgca
    24181 gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ccattcctca tggatgggcc
    24241 gtttatttcc ccgctctacc ttcgttatat ccaggcagat tttttaactg actttcgttt
    24301 gaaaactggc gtttttccaa aactggatta caaataaata tataaaaaac aatgcgttta
    24361 tccgccgtta atttcatgta agtcatgttg ttccttttat ttaaccgcgg actccgctgt
    24421 taaccggagg atatgcatca tgaaaaccgt tagggagtcc acaacgttgt acaactttct
    24481 cggatcgcac aatccatact ggcggttgac ggaaagcagc gatgctttgc gcttttctac
    24541 caccaaaacc acagaacctg atcgtacatt gcagttatct gccgaacagg ctgctcgcat
    24601 cagggaaatg acggtcatca cctccagcct gatgatgagt ctgaccgtcg atgaaagcga
    24661 tctttctgtg catctggtag gacgaaaaat caataaacgg gaatgggctg gcaacgcgtc
    24721 tgcatggcat gacacaccgg cggttgctcg tgatttatca cacgggcttt cctttgctga
    24781 gcaggtagtt tctgaagcac attccgcaat agtgattctc gacagccggg ggaatatcca
    24841 acgcttcaat cggttatgtg aagattacac agggttgaaa gaacacgacg tcattgggca
    24901 aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg gcatccaggc gcaataaccg
    24961 tgtatttttt cgaagcggca atgcatatga agtcgaactg tggataccaa cacgtaaagg
    25021 ccagcggctg tttctgtttc gcaataaatt tgtccacagc ggcagtggca aaaacgagat
    25081 ttttttaatc tgttccggta ccgacattac cgaagagcgc cgcactcagg agcgactgcg
    25141 tattctggca aataccgaca gtatcaccgg actgccgaat cgtaacgcaa tgcaggagtt
    25201 aatcgatcac gctattaatc aggcagataa caataaagtt ggggttgtgt atcttgattt
    25261 ggataatttt aaaaaggtca acgacgccta tgggcatttg tttggtgacc agttattacg
    25321 cgacgtgtca ttggctattt taagctgtct cgaacatgac caggtgttgg cgcgtccagg
    25381 tggcgatgag tttctggtac tggcatccaa tacctcacaa agcgcgctgg aagcaatggc
    25441 atcacgaatt ttgacccgct tacgcctccc ctttcgtatt ggtttaattg aagtttatac
    25501 cagctgttca gtaggtattt cgctctctcc cgaacatggt tcggacagcg cgactattat
    25561 tcgtcacgcc gacacagcaa tgtacacagc gaaggaaggc ggacgaggac aattttgcgt
    25621 ttttactcca gaaatgaatc agcgggtatt tgaatatctc tggctggata ccaacttgcg
    25681 taaagcactg gaaaacgatc agttggttat cgaaaatcac ctagcgtggc gaagtgcgca
    25741 gtctggaagc actagtacgt tggcagtcac ctgaacgtgg gttgattcca ccgttggact
    25801 tcatttccta cgccgaagag tcagggctaa ttgtgccttt aggccgttgg gtgattctcg
    25861 atgtcgtacg ccaggtggca aagtggcggg ataaaggcat aaacctgcga gtggcggtaa
    25921 atatttctgc acgtcagctc gccgatcaaa ccattttcac cgccctgaaa caggttctcc
    25981 aggaactcaa ttttgaatac tgccctatag atgttgaact gacagagagt tgtctgattg
    26041 agaatgatga actggcactg tctgttattc aacaatttag ccaactaggt gcgcaagtgc
    26101 atctggatga ttttggtacc ggctactctt cactttcgca actggcgcgc tttccgatcg
    26161 atgccatcaa acttgaccag gtttttgttc gagatattca caaacaacct gtctcgcagt
    26221 cactggtccg ggcgatcgtc gctgtggccc aggcattgaa tcttcaggtg atcgccgaag
    26281 gtgtagagag tgcaaaggaa gatgcttttt taaccaagaa cgggatcaat gagcggcaag
    26341 gatttttgtt tgccaaaccg atgcccgccg tcgccttcga acgctggtat aaacgctatc
    26401 tgaagcgcgc ataagtagat gaatatatat acttagcgct atatcccgtc attatatatc
    26461 gtattcctgg atgataatta gccgttgctg ttaaacactt ctcttttagc ggcaattttt
    26521 gcattattca ttttccccct tttgctataa ggatgtcctt gatgtctgaa tttgatgccc
    26581 agcgcgttgc cgaacgtatt gatattgtgt tagatatcct tgtggcaggc gattaccact
    26641 ctgcgatcca taatctcgag atacttaaag cagaactctt gcgccaggtt gctgaatcta
    26701 cgccagacat cccgaagaca ccgtgggaga tttaacaatc gtgccttcag gttttcctat
    26761 ttgaggatgc ccggcgcgtc agtatttttt tatttagtat tataacgtta taagaattac
    26821 agcgatgaat tcccgacaac aaactattct acagatggtc attgaccagg gtcaggttag
    26881 cgtaaccgat ctggcaaaag ccactggagt ttctgaagtt accattcgcc aggatctcaa
    26941 caccctcgaa aaactgagtt acctccgccg tgcacatggc tttgcagttt cgcttgatag
    27001 tgatgacgtc gaaacccgta tgatgagcaa ctatacgctg aagcgtgaac tcgccgagtt
    27061 tgccgcgtca ctggttcaac cgggcgaaac catctttatc gaaaatggca gcagcaatgc
    27121 cctgctcgcc cggactctgg gggagcagaa gaaaaatgtc actatcatca cggtcagcag
    27181 ctacatcgcg catttgctga aagacgcgcc ttgtgaagtc attttgctcg gtggcgtgta
    27241 ccagaaaaaa agcgaaagta tggttggccc tttgacacgc cagtgcatcc agcaggtaca
    27301 tttcagcaaa gcatttattg gtattgatgg ctggcaacct gaaactggat tcaccggtcg
    27361 cgatatgatg cgcaccgatg tggtcaatgc cgtgctggaa aaagagtgcg aagcgatagt
    27421 cctgactgac agctcgaaat ttggtgctgt acattcatac tccatcggtc ccgttgagcg
    27481 attcaatcgc gtgattaccg attcgaaaat acgcgccagc gatctgatgc atcttgagca
    27541 cagtaaactc accgttcacg tcgttgacat ttaaccattt cccttctcta cggatgattt
    27601 gcagtttggc aaatcatccg ctctaagatg attcctggtt gataattaag actatttacc
    27661 tgttattaac actctcaaga tataaaatta ttatcagcga tataacagga agtaattatc
    27721 acgtgcgt
//
LOCUS       DAJTNM010000041        27713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTNM010000041 DAJTNM010000000
VERSION     DAJTNM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.041"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..614)
                     /locus_tag="OO172_001890"
     CDS             complement(<1..614)
                     /locus_tag="OO172_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5512093.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAG"
     gene            complement(611..1873)
                     /locus_tag="OO172_001891"
                     /pseudo
     CDS             complement(611..1873)
                     /locus_tag="OO172_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(1870..2778)
                     /locus_tag="OO172_001892"
     CDS             complement(1870..2778)
                     /locus_tag="OO172_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5512094.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2974..3741
                     /locus_tag="OO172_001893"
     CDS             2974..3741
                     /locus_tag="OO172_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5512095.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(3792..4444)
                     /gene="pphB"
                     /locus_tag="OO172_001894"
                     /pseudo
     CDS             complement(3792..4444)
                     /gene="pphB"
                     /locus_tag="OO172_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(4550..7111)
                     /gene="mutS"
                     /locus_tag="OO172_001895"
     CDS             complement(4550..7111)
                     /gene="mutS"
                     /locus_tag="OO172_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5512096.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLLRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            7187..7366
                     /locus_tag="OO172_001896"
     CDS             7187..7366
                     /locus_tag="OO172_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512097.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            7398..7751
                     /locus_tag="OO172_001897"
     CDS             7398..7751
                     /locus_tag="OO172_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5512098.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(7788..9866)
                     /gene="flhA"
                     /locus_tag="OO172_001898"
     CDS             complement(7788..9866)
                     /gene="flhA"
                     /locus_tag="OO172_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5512099.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(9940..10950)
                     /gene="hypE"
                     /locus_tag="OO172_001899"
     CDS             complement(9940..10950)
                     /gene="hypE"
                     /locus_tag="OO172_001899"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5512100.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(10947..12068)
                     /gene="hypD"
                     /locus_tag="OO172_001900"
     CDS             complement(10947..12068)
                     /gene="hypD"
                     /locus_tag="OO172_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5512101.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(12068..12340)
                     /gene="hypC"
                     /locus_tag="OO172_001901"
     CDS             complement(12068..12340)
                     /gene="hypC"
                     /locus_tag="OO172_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5512102.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(12331..13203)
                     /gene="hypB"
                     /locus_tag="OO172_001902"
     CDS             complement(12331..13203)
                     /gene="hypB"
                     /locus_tag="OO172_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5512103.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(13207..13557)
                     /gene="hypA"
                     /locus_tag="OO172_001903"
     CDS             complement(13207..13557)
                     /gene="hypA"
                     /locus_tag="OO172_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5512104.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            13769..14230
                     /gene="hycA"
                     /locus_tag="OO172_001904"
     CDS             13769..14230
                     /gene="hycA"
                     /locus_tag="OO172_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5512105.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            14355..14966
                     /gene="hycB"
                     /locus_tag="OO172_001905"
     CDS             14355..14966
                     /gene="hycB"
                     /locus_tag="OO172_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5512106.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPACVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            14963..16821
                     /gene="hycC"
                     /locus_tag="OO172_001906"
                     /pseudo
     CDS             14963..16821
                     /gene="hycC"
                     /locus_tag="OO172_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            16824..17720
                     /gene="hycD"
                     /locus_tag="OO172_001907"
     CDS             16824..17720
                     /gene="hycD"
                     /locus_tag="OO172_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5512107.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            17738..19423
                     /gene="hycE"
                     /locus_tag="OO172_001908"
     CDS             17738..19423
                     /gene="hycE"
                     /locus_tag="OO172_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5512108.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTAFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            19433..19975
                     /gene="hycF"
                     /locus_tag="OO172_001909"
     CDS             19433..19975
                     /gene="hycF"
                     /locus_tag="OO172_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR5512109.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KENFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            19975..20742
                     /gene="hycG"
                     /locus_tag="OO172_001910"
     CDS             19975..20742
                     /gene="hycG"
                     /locus_tag="OO172_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR5512110.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            20739..21149
                     /gene="hycH"
                     /locus_tag="OO172_001911"
     CDS             20739..21149
                     /gene="hycH"
                     /locus_tag="OO172_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR5512111.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            21142..21612
                     /gene="hycI"
                     /locus_tag="OO172_001912"
     CDS             21142..21612
                     /gene="hycI"
                     /locus_tag="OO172_001912"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR5512112.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            21682..22614
                     /locus_tag="OO172_001913"
     CDS             21682..22614
                     /locus_tag="OO172_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512113.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEGGTRTDFLQSGYEDFGGNYIAHKCLHIVNL
                     DLMSYSFRTHKDRTRRCGCLLTIRKLKLKNAHHF"
     gene            22598..22909
                     /locus_tag="OO172_001914"
     CDS             22598..22909
                     /locus_tag="OO172_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR5512114.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            22906..23325
                     /locus_tag="OO172_001915"
     CDS             22906..23325
                     /locus_tag="OO172_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5512115.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(23440..24864)
                     /gene="ascB"
                     /locus_tag="OO172_001916"
     CDS             complement(23440..24864)
                     /gene="ascB"
                     /locus_tag="OO172_001916"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR5512116.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAIGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24873..26321)
                     /gene="ascF"
                     /locus_tag="OO172_001917"
     CDS             complement(24873..26321)
                     /gene="ascF"
                     /locus_tag="OO172_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR5512117.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQPTVAKEVSLN"
     gene            26581..27591
                     /gene="ascG"
                     /locus_tag="OO172_001918"
     CDS             26581..27591
                     /gene="ascG"
                     /locus_tag="OO172_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5512118.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
BASE COUNT         6350 a         7330 c         7456 g         6577 t
ORIGIN      
        1 cctgcggtca gataacggat cgggcggtca ccgagaataa aaatcagctt tgccgtttcc
       61 tccagctctt ccatattgtt ggcggcttct tgcaggcttt caccgcaaac cactgggcca
      121 tgatttgcca gtaaaaaagc ctgattgtct gctgccagtt ccgccagatc ctgtgcgatg
      181 cgtttatcgc ccggtcggta atagggcacc agcgggacat ttcccatccg catcaccacg
      241 tatggtgtga acggacgaat aacgttgctg ctgtccagcc cttgcaggca ggaaagcgcc
      301 gtcgaccatg tgctgtgcaa atgcaccacc gctttacagc gcggattgtt gcgatacagc
      361 gccagatgaa agagcacctc tttcgagggt ttgtcaccac ttaaccattc gccatccgcg
      421 gcgactttgg aaagccgctg cggatcgaga ttgcccaggc atgaacctgt cggtgtcgcc
      481 agtaaattcc cgtcaggtaa aagcagcgac agattgccag ccgaaccggt tgcatagccg
      541 cgctgaaaga atgaactggc aatccgcgtc atctcctctc gcaaagactg ctctactttt
      601 gcgaaatcgc tcatgataaa aactctcttt gggctcgtga aaaaaaggct tcatcaccga
      661 agttgccaga tttaagggcg agtgagacag gcttatccag tgcgtttacc cacggcacgc
      721 cgggggaaat ggttgggcca atatgaaacc cttttatgcc caggctctgt gtgactacgc
      781 cggaggtctc accacctgcg acaataaagc gtgtcatgcc ttccgctgct aaccgcgccg
      841 ctagttgaga aaacagagtt tctactgcct gactggcttt ttgtgcaccg tattgctgtt
      901 gaattgctgc caatgcgtca gtgctggcgg tggcaaaaac cagtggagca agtacacttt
      961 cctggcccag aacccactct gccagttcgt gagcataagc ggccagagtt tcggttgaga
     1021 ggcagcgtgc cacatcaact tcacgggctg gtgcaatttg acggtaatgt gccacctagc
     1081 ggttggtcat ttgagagcat gaaccggaga gcactacgcc gcgcccagcg agcggacgcc
     1141 ctgcttcgcg agcctggtta ccgttttctt gcgcccactg ccgggccagg ccaatcgcca
     1201 gaccagaacc gcccgttacc agtggggcat cgcgcaaggc ttctccctga atttccagat
     1261 ggtgttcggt cagcgcatca agcaccgcgt agcggtagcc ctcttgctgt aagcgagcca
     1321 gctcttgacg aacggcatcc acaccttgtt cgaaaacatg tgccgaaacg acgccgcagc
     1381 gccctgtgga ttgcgcttca accagacggg gaagatagct gttggtcatg ggatttaccg
     1441 ggtgatggcg catcccggat tcggccagca gttgattcat tacgaacaaa tacccctgat
     1501 aaaccgtacg tccgttgacc ggcagggccg gagagaagac cgtaaacggc gtgtcgagag
     1561 catccattaa tgcatcggta accgggccaa tattaccttt cgccgtactg tcgaaagtag
     1621 agctgtattt gaaatagatc tgtttgcaac cttgctgttg caaccagctc agagccgcca
     1681 gcgattgctg tgtggcttca accaccggac aggagcgcgt tttcaggctg atcaccagtg
     1741 cgtcgattgc ttccggcatt ttacctgttg gaacaccgtt aatttgtacc gttggtagac
     1801 cgttttccac cagaaaactg gcgatatccg tcgcgccggt aaaatcatcg gcgataacgc
     1861 caatcttgat catgatttcg ctcccggtag agtgatgcca gagaaaatct tgataactgc
     1921 gctatcgtct tctttcccgt aacccgcgtt actggcgctg gtgaacatat tcaatgctgt
     1981 tgaggccaat ggcagcggga agtgcagggc tttggctgta tcggcaacca gaccaagatc
     2041 cttaacaaaa atatcgacgg ctgaatgcgg ggtgtaatcg ccatccacca catgacgcat
     2101 ccggttttcg aacatccagg aatttccggc ggcattggtc acgacgtcat acatcacatc
     2161 cagcgggatc cccgcacggg ctgcaagtgc catcgcttcg gctccggcag caatatgtac
     2221 gcccgctaac aactggtgaa taatttttac ggtcgaacct agtcccggtt ctgcacctat
     2281 gcgataaact tttccggcaa cggcttccag cacgggtgcc agtcgttcaa aggcaatatc
     2341 gctaccggag gccatgacag tcatttcacc gttagcggct tttactgcac caccagaaac
     2401 tggcgcatcc agcatttcca gatcgaatcc agccagagcg gtagcaattt cttgcgcatc
     2461 agcactagcg atagtggaag aaaccattac tgccgtaccg ggtttcagat gttgtgcaac
     2521 gcctgtttca ccaaacagca cctgtttaac ctgggccgca ttgaccacca gcaccagcag
     2581 agcgtccagt ttttcggcaa acgtcgcggc gttatcagaa accccgcaag cacctgcctc
     2641 tttcaacgta gcgcaggcat tgctgttcag gtctgcgccc caggtagaaa aacctgcgcg
     2701 gacacatgac agtgctgctc ccattcccat tgaccctaag ccaacgatac cgacatgaaa
     2761 ctcagatccc gttttcatct gctctccttg ttaatttaag tgatattttg tttgacattg
     2821 tgaatataag cgctgggaga taacgatatg gtgagctgat tcacataaat taacattttg
     2881 tgttatttta tgtgaactaa gcgttagttg cgccgcgcac gtttcgcagg caaatagcgt
     2941 agaatgtcag caggacaaag gaaggagcaa aagttgatac ccgtagagcg tcgccaaatc
     3001 atccttgaga tggtagctga aaaaggcatt gtcagtattg ctgaactaac ggacagaatt
     3061 aatgtgtcac atatgaccat tcgtcgggat ttacaaaaac tggagcagca gggagccgtt
     3121 gtgctggtgt ccggaggcgt ccagtctccg ggacgcgtgg cgcatgaacc ttctcatcag
     3181 gtaaaaactg cgctggcaat gacgcaaaaa gcggctattg gcaagctggc ggcaagtctt
     3241 gttcagccgg gaagttgtat ctatctggat gcgggaacga ccacgttagc gatagcacag
     3301 catctgattc acatggagtc actgactgtg gtcacaaacg atttcgttat tgcgaactac
     3361 ttgctcgaca acagtaattg cacaattatt cacactggcg gtgcagtgtg tcgggaaaac
     3421 cgttcctgtg tcggggaagc cgctgcgacc atgctgcgca gcctgatgat tgatcaggct
     3481 tttatttctg catcgtcatg gagtgtgcgg gggatttcta cgccagcgga agataaagtc
     3541 acggtgaaac gggcgattgc cagtgctagc cgccagcgag ttttggtctg tgatgcgacg
     3601 aaatatggtc aggtggcgac atggctggcg ttaccattaa gcgagtttga tcagattatt
     3661 acagacgacg gtttgccgga gagtgccagt cgcgcgctgg cgaagcagga tctctctttg
     3721 ctggtagcga aaaatgaata atggcctgca ataacatctg gttactcatg cttcacagaa
     3781 gaagcatgag actaccttat tttataaaat gacagccgcc cacttttcgg cgatccggta
     3841 tcaatataaa tctggttagc gaacgtctga atgttatcaa acatcatatg tccaaatata
     3901 aaataatcag cgccgtttat ttgttgtaac tcgccattaa gcgagttctg cacacgataa
     3961 acaggccaga gtaattcgct ctccgctatt tctttgtcaa agagatattc atcacccgga
     4021 taatctgcat gtgcgatgac atattttatt atgtcgttag tgatttcaat aatatgtgga
     4081 aggtgatgga atttcagcaa cagatctatt gcctcttgtt gcaatcattt aaatcgaaaa
     4141 accagtcacc accgctggca agccacatat tgccatcgcc ggtcgcgaac gcatccagcg
     4201 ccattgcttc gtggttgcct ttaacggaga taaaccaggg ctggtttagc aggcgcagga
     4261 cgtcaagact ctccggccca cgatcaatat tatcgccgac agaaataagt aagtcggttt
     4321 cggggaaaaa agagagttga tgtaagcggg attgtaataa ctgatattca ccatgaatat
     4381 caccaacgac ccatatatgg cgatagtgat gggcattgat tttttgatag cgtgtagatg
     4441 gcatggtttt accctgtaaa ataagctttc ctattataca gggtattttt atttgattcg
     4501 tcagttgtcg ttaatattcc cgatagcaaa agactatcgg gaattgttat tacaccagac
     4561 tcttcaagcg ataaatccac tccagcgcct gacgcggagt cagtgaatcc gggtcgagat
     4621 tttccagtgc ttcgaccgca ggcgaagttt cttctggcac tgacagcaaa gacatctgcg
     4681 taccatccac ttgcgtcgcg gcggcgttcg gcgaaatgct ttccagctca cgcagttttt
     4741 gccgtgcgcg cttaataacc tctttcggta cgcccgccag agcagcaacc gccagaccgt
     4801 agcttttgct cgccgcgccg tcctgcacgc tgtgcataaa ggcgatagtg tcgccgtgct
     4861 ccagcgcatc gagatgtacg ttggcgacgc cttccatttt ctccggtagc tgggtcagct
     4921 cgaaatagtg ggtggcaaac agcgtcaatg ccttaatctt attcgccaga ttttctgcgc
     4981 acgcccacgc cagcgacaga ccatcgtagg tggacgttcc acgcccgatc tcatccatta
     5041 acaccagact gtattcggtg gcgttatgta aaatattggc ggtttcagtc atctccacca
     5101 taaaggttga gcgcccggaa gccaggtcat ctgccgcgcc tacgcgggta aagatgcgat
     5161 cgataggtcc aatctcgact ttttgtgccg gtacatagct gccgatgtag gccatcagcg
     5221 caatcagtgc ggtctggcgc atataggtac ttttaccgcc catgttcggg ccggtaataa
     5281 tcaacatacg gcgctgcggc gacagattca gcgggttagc gataaatggc tcattcagta
     5341 cttgttcaac taccggatgg cgaccttcgg taatgcgaat gcccggttta tcaatgaagg
     5401 tcgggcaggt gtagttcagg gtataggccc gttccgccag gttaaccagc acgtcgagtt
     5461 ccgccagcgc gctcgcgctc tgttgcaacg cttccagatg cggcaacagc aggtcgaaca
     5521 gctcttcata aagctgtttt tccagtgcca gtgctttgcc ttttgaggtg agaactttat
     5581 cttcgtactc ttttagctct ggaatgatgt agcgctcggc gtttttcagc gtctggcgac
     5641 gcatgtagtt gatgggtgcc agatggcttt gcccacggct gatttgaatg tagtagccgt
     5701 gcaccgcatt aaagccaact tttagcgtgt ccaggccggt acgttcacgc tcgcggactt
     5761 ccagacgctc cagataatcg gtcgcgccgt cagccagcgc gcgccactca tccagctctt
     5821 cgttatagcc cgatgcgata acaccaccgt cgcgtaacag caccggcggt gtgtcgatga
     5881 ttgctcgctc cagcagatcg cgcagctcgg caaactctcc catcttctca cgtagcgcct
     5941 gtaccggtgc actatcgaca gtttctaact gcgcacgcag ctccggcagt tgctggaaag
     6001 cgtggcgcat acgggccaga tcgcgtgggc gagcagttcg taaagccaga cgtgccagaa
     6061 tacgttccag gtcgccgacc tgacgcagta ccggctgcaa ctcggcggtg aaatcctgca
     6121 atgcgccaat agtttgctgg cgctcaagca acacgcgagt atcgcgcact ggcatatgca
     6181 gccagcgttt cagcatacga ctacccatcg gcgtgacggt gcagtcgagc acagaagcca
     6241 gcgtattttc cgcaccgccc gccaggttct gagtaatttc caggttacga cgtgtcgcgg
     6301 catccataat gatgctgtcc tgctcacgtt ccatggtgat agaacgaata tgcggcaggg
     6361 tcgtgcgttg ggtatctttc gcatactgca acagacaacc ggcagcacaa agtccgcgcg
     6421 gcgcgttctc gacgccaaaa ccgaccagat cgcgggtgcc aaattgcaga ttcaactgct
     6481 ggcgcgcggt gtcgatttca aactcccaca gcgggcgacg gcgcaggccg cgacggcctt
     6541 caatcagcga catctcggcg aaatcttctg catacagcag ttccgccgga ttagtgcgtt
     6601 gcagctctgc cgccatcgtt tcgcggtcgg ccggttcgct caggcgaaaa cgcccggagc
     6661 tgatatccag cgtcgcgtag ccgaaacctt tgctgtcctg ccagatagcc gccagcaggt
     6721 tgtcctgacg ctcctgtaac agggcttcat cgctgatggt gcctggcgta acgatacgca
     6781 caactttgcg ctcaaccgga cctttgctgg tcgccggatc gccaatttgt tcgcagatgg
     6841 caacggactc tccctgattc accagtttgg cgagatagtt ttccaccgca tggtagggaa
     6901 tccccgccat cgggatcggc tctcccgccg aagcaccgcg tttggtcagt gaaatatcca
     6961 gcagttgcga cgcgcgtttt gcgtcgtcat aaaacagttc ataaaaatca cccatccggt
     7021 aaaacagcag gatctcggga tgctgggctt tcagcttgag atactgctgc atcatgggcg
     7081 tatgggcgtc gaaattttct attgcactca tggggttatg tccggttccc tgatattaaa
     7141 tggggtgtga tggtttttat tttcgttgta atcacataag gcgcacatga taccggagtt
     7201 aatcatttca ggggaagaag cgaacggcga gttcttgatg ctggttccgg gaaagatgct
     7261 gttttgcagc ttttgcgtga tgcttatgaa gcaagtacgc aaaattgtat cgattggcga
     7321 acccttcttc tttagagtca tagtgccctt taagattaag atgtaatata aatgcatctt
     7381 aatgaggtgg aggcagcatg tccggcaagc gtatctctcg tgaaaaactg acgattaaaa
     7441 aaatgatcga tctttatcaa gcgaaatgcc cgcaggcgtc agcggagccg gagcattacg
     7501 aggcgttgtt tgtttacgcg caaaagcggc tggataaatg tgtgttcggc gaagagaaac
     7561 cagcctgtaa gcagtgtccg gtgcactgtt accagcctgc aaagcgtgaa gagatgaaac
     7621 agattatgcg ttgggccggg ccgagaatgc tgtggcgtca tccgatctta accgtgcgtc
     7681 atctcatcga tgacaagcgt ccggtgccag aactgccgga aaaatatcgt ccgaaaaaac
     7741 ctcgtgaata gtatgccgga caggcagatc tgtccggcaa tttgcagtta aatcaatgcc
     7801 gatttatcaa ttcccagccg cttcatccgt gacagcaggg tcgtgcgttt cagccccaga
     7861 cgttgcgcag cgcctttagg cccggcaacc acgctgttgg tttctttcag cacgcgcaca
     7921 atcaactgat attcatcttc gccctcctgg gcgacaaccg ttgcggcagg cggcgtttca
     7981 ggctccggta aagcaatatc tggcaatgac agctgcagca cgttgccgcg tgttagcaat
     8041 actgcgcgct caatgacgtt ttccagttcg cgtacgttac ccggccactc catgttgctc
     8101 aaggtgcgca gcgtctcggc aggaatgctg tcgatattgc gccccagacg acgggcaatt
     8161 ttgaaggtaa aggctttcgc cagcagcgga atatcttccg gacgctcgcg tagtggcggc
     8221 aggtgaatcg ggaatacgtt caggcggtaa tagagatcgc tacggaactc tcggtcggcg
     8281 accatttttt tcagatcgcg gttagtcgcg gcaattaaac gtacgtccgt ctgaatgatt
     8341 ttgttgctgc cgagacgttc aaactcctgt tcctgcaata cacgcagcaa cttcggctgt
     8401 aactccagtg gcatatcgcc cacttcgtcg aggaacagag agcttttatc cgccagttca
     8461 aaacgaccaa tgcgctgggc gctggcaccg gtaaaagccc cacgctcatg accaaacaga
     8521 tcgctttcca gcaatccggc aggcatcgcc gcgcagttca ttttgaccat gcggcgatta
     8581 ttacgcccac tgagattatg gatcgcacgg gcaatcagct ctttacccgt gccagtttca
     8641 ccgaggatca gcacggtact gtcactttgc gccaccattt caacttgttt aagcacgctg
     8701 tacatggctt cgctgcggcc aataatctcg ccaaattcac tatcaacatt gttgagttgc
     8761 tcggtcaggg cgaggttttc atcaaccagc cgttctttca gacgatggat ttcctgatag
     8821 gcgagggcgt tatcgacagc gattgccaca cgttcggcaa tctggcgcag caaattcaga
     8881 ttggtagtgg taaacacttt ctcttcgcat tgcgccagtt tcagcacgcc cagcatggtg
     8941 tcgccagaca tcagcggtaa caggcacaag gtttgaatct ggttgcccca ggtgtcgaac
     9001 aacatgcgtt catagggggc taaatcgtcc cgctcgtaga gattgatcag cagcatctct
     9061 ttacttttga acacgcgttc ggtgagggtt ccggcttcat cgacttcgct ctgttcgtgg
     9121 gcgggatgtt gtttatcaag atagtgagtg gagtagatgt tgagtttgtt tttacggtgg
     9181 ctgcgtaaga caatgctgat atcgtcaatg tcgaaatagt aatggatttc tttggcgact
     9241 tcgctgacca gttcgtccat atccaggcgg gaaagcaccg cgttggtaat ggcaaccagg
     9301 atgcggaagt tatcgcgctc ccggcataac aactcatagt cgacattgtt gacgacgcgg
     9361 ctctggattt gttcggtgac gacagaaacg atctgcgtaa atgtttgcag acgattgaac
     9421 tctttttcgc tccagggacg atcgtcataa cgaataaatt cacagccacc aaaaatatgc
     9481 cctcccgccg ccagtggcat caggcaatag tgaccaaatt tggggtatag cccacctgcg
     9541 gccagctgcg gccaggtttc acaaaattct tcgtaactgc aatgcagcgt atcagggcgc
     9601 gacaaaatgc tgcgtaccgg accgtgtgcc agaacagttt cgtcttcata tttaatgggg
     9661 gtgtcttttt cacgcgacgc gtaataagac gcacgttgag tctgcgcttg ccacaacaca
     9721 atagccgcgt tgtcggcgag cgcagaacgc tttaccagtt gcgaaagagc ctcacacagc
     9781 gaggccagat cgggctgctg caatagtgtc cgagtgatgt cgaacaaccc ttgctgtccg
     9841 agatcactca tcggtgtata tgacattttg ttcttccagg aaagcaccgc aaagcggtgc
     9901 aaaaggtcat tgactaacta taggagattt agaattttat tagcatatac gcggaagcgg
     9961 ttcggcgtgt ggtaaatcga gggttcgttt cacgccatac agaccggcaa gacgaacacc
    10021 tttacgttcc accacttcac caatcagcgc cgcgtctttc cccagtggat gggaatgtaa
    10081 cgctgccagc acttgctctg ccgcgttgcg ttcaacggcg atcaccagtt taccttcgtt
    10141 ggcaaagttc agggcgtcca gtcccagcaa ttcgcaaacg ccgcgcacgg caggtttaac
    10201 cggcagcgct gattcagaaa tttcaatacc acaaccgcag gctgccgcga actcatgaac
    10261 caccgcgttt acaccaccac gggtggcatc acgcagcgct ttcacgccgg gaatgtcacg
    10321 cagcgtctga ataagcggcg tcagcaccgc gcagtcgctg accagttcgc catccagccc
    10381 cagctgctca cgcaggttaa ggatagtcgc cccgtggtcg ccgagtgtac cgctaaccag
    10441 caatacatcg cctgcggtta gcgtctgtgc gccccagtga atattcgccg gaattgcgcc
    10501 catgccagcg gtgttgataa acagtttatc tgccgcgccg cgctgcacca ctttagtatc
    10561 gccagtaacg atggcaatgc ctgccgtgcg ggcggtttct gccatgctgg tcactgcggc
    10621 tttcagtgtc tccatcggca atccttcttc gaggataaag ccacaggaga gatagcgcgg
    10681 aatagcgcca ctgaccgcaa cgtcattcgc ggtgccgcaa atcgccagct tgccgatatt
    10741 accgccagga aagaacagcg ggtcaatgac gtaactgtcg gtggagaacg ccagacggtc
    10801 gccttccgct accagctgcg ccagatcaag acgtgcctga tcttcctgct ctgccagcca
    10861 cgggttggca aaggcttcca taaacaggct gttgattaat tgctgcatcg cctggccgcc
    10921 gctgccgtgg gcgagttgga tattattcac gcttcactct cctgctgacg atactgatac
    10981 cacgcggcgc acgctccttc ggaggagacc atcagtgcac caaatgcggt ttgaggatta
    11041 caggtgttac caaacagcgg gcattgatgc ggcttacatt tgcccgttaa tacctcacca
    11101 caacgcgcgc gcgggtcatc gcagacctgc tgcggtgccg ggcggaaatg tgcttcggca
    11161 tcgaatcgtt gataatccgg cgtcaggtgc acgccagaag attcaatcac gcctaagccg
    11221 cgccattcgc tgtcgccgtt gacacagaac acatcggcaa tcgcctgttg cgccagcagg
    11281 ttaccggcat ccggtaccac ccgacgatac tgattctcta ccttgctgtg ggccgctatt
    11341 ttctgctcca ccagcatgac cacgccttgc agtagatcaa ggggttcgaa accagcgacc
    11401 accagcggac gatgaaaatc gctggcgata aaattatagg catcagtgcc gataaccata
    11461 ctaacgtggc ctggcgcgag gaacgcgtcg ataccgttat ccggctgttc cagcaaactg
    11521 cgcagcgtcg ggataagcgt aatgtgctgg cagaagaagt aaaaattctg cacatcacgc
    11581 gctttcgcct gttgcagagt gatggcggtg gtcggcatgg tggtttcaaa acctaagccg
    11641 aagaacacca ctttgcgggt tggattctcc tgcgccagtt tcaacgcatc catcggcgag
    11701 taaacgatgc gcacatcggc accgcgtgct tttgcctgca acagcgatcc ctgtttccct
    11761 ggtacgcgca tggcgtcgcc aaaggtacag aagatgactt ccggatggct ggcaatctcc
    11821 acgcaggtgt cgattctacc catcggcagt acgcacaccg ggcaacccgg accgtggata
    11881 aactcaacgt tttcaggcag taactggtcg aggccgaatt taaaaatggc gtgggtatga
    11941 ccgccacaca cttccataat ccgcagaggg cgttcggcgg tgtaagagag atgtgaagca
    12001 cgttcgcgca gatgctcaat taactgcatc acctgttccg gcgcgcgata ttcatcaaca
    12061 aaacgcatta tttttcctcg ccatacaaca gcgcaccgac atccggctca acgtcaaaca
    12121 tgttttgtaa ggcgtcgaga gtgtcgcgtg cttcggcttc attaattacg ctcatggcaa
    12181 agccaacgtg aaccagtacc cactggccca cgcgcggctg accgttttca tcgcagctgc
    12241 cgactaacgt taaatcgaca tcgcgctgaa tgccgcagac gtcgactttc gcctggttgc
    12301 cgtcaatggt gcggatctgg ccgggaacgc ctatgcacat cgctgtgtct ccagccagtt
    12361 cagccactgg tccatccctt cgccgctggt ggcggaaata aggatgattt caatttctgg
    12421 attgacttcg cgggcgcagg cgatgcactt ctcaacgtca aagttgagat acggcaacag
    12481 gtcaactttg ttgagcagca tcagcgaggc ggcggcaaac atatgcggat atttcagcgg
    12541 tttgtcttca ccttcggtaa cggaaagcac cgctactttg tgtttttcac cgagatcgaa
    12601 gctggccggg catacgaggt tgccaacgtt ttcgataaac agaataccgt tatcgtccag
    12661 tggcagacgc ggtgcggcgt cggcaatcat ctgtgcgtca agatggcagc ctttaccggt
    12721 gttcacctga atcgctggtg taccggtagc gcgaatgcgt gcggcatcgt tcacggtttg
    12781 ctggtcgcct tcaataaccg cgcacggaac gctgtctttc aggcgcatta aggtttccgt
    12841 cagcagggtg gttttaccgg aaccagggct ggaaaccagg ttgagcacca gttgcttgcg
    12901 ggcagcaaag cgcgcgcggt tgcgttcagc cagacggttg tttttgtcca gcacgtcaat
    12961 ttcgacttcc agcatccgac gctggctcat gcccggtgtg tgagtgcccg cttcgccatg
    13021 accgtaatgc aggtcgcctt cttcagtctg gctgggggta aattcaggcg ctttaatgcc
    13081 ggtgattttc atctgcgggc gtgccgccgg ggcaaatggc gcgctacgaa acgcggaatg
    13141 agggttatgt tcatcaccct cgatatacag gttgccttca ccgcaaccgc atgttgtaca
    13201 catcgctcac tcctggtcta tttctatccg ccgaatctgt aaaccgtcgt ctgccacaat
    13261 ctgtagcatg tcaccatgac actgtggaca gcggcggacg cgctgggtca gtagcgtcac
    13321 atactgttgg catgtttcac accagcattc ggcctcttgt tcttcgaggt gcagtttaca
    13381 accttccgcc acgctgccgc ggcaaaccag atcaaaacaa aaggcaagag agctggtttc
    13441 gacacaagaa aatgcgccaa ttttgagcca gaccccagtt acgcgttttg cgccgtgttt
    13501 tgcggcctgc tgttcgatca attccagtgc ccgttggcag agggttattt cgtgcatatc
    13561 gcctcccatt aactattgcc agctacaagc aataattgcg ccagtgttga ttatccctgc
    13621 ggtgaataat gtcgatgatg tcgaaatgac acgtcgacac ggcgacgaaa ttcatcttta
    13681 gcttaaaaat ctctttaata acaataaatt aaaagttggt acaaaaaatg cttaaagctg
    13741 gcatctctgt taaacgagta acctgacaat gactatttgg gaaataagcg agaaagccga
    13801 ttacatcgca cagcggcatc gtcgcctaca ggaccagtgg cacatctact gcaattcgct
    13861 ggttcagggg atcacgttat cgaaagcgcg cctgcatcac gccatgagct gcgcgccgga
    13921 caaagaactc tgtttcgtcc tttttgaaca ttttcgtatt tacgtcaccc tggcggatgg
    13981 ctttaacagc cacaccatcg agtattacgt cgaaacaaaa gatggcgaag acaaacagcg
    14041 gattgcgcag gcgcaactga gcattgacgg catgattgat ggcaaggtca acatccgcga
    14101 tcgcgaacag gttctggaac actatctcga aaaaatcgct ggcgtttacg acagcttata
    14161 caccgccatt gaaaacaatg tgccggtgaa tttaagccaa ctggtaaagg gacaaagccc
    14221 ggcagcatga gctgaggctt tgcccgtttt gcaggcgtta cgcctgtttg gggatgggcg
    14281 tgtcgatgag tgtcgaaaat gacatttcat cggcatgttt tcgtcaaaaa tgacaatcac
    14341 ctgaggaatg cctggtgaat cgttttgtaa ttgccgactc cacgctctgt atcggctgcc
    14401 acacctgtga ggccgcctgt tcagagacgc atcgccagca cggcctgcaa tcaatgccgc
    14461 gcctgagagt gatgctcaat gaaaaagaat ctgcgccgca gctctgtcac cactgtgaag
    14521 atgcaccctg cgcggtggtc tgcccggtta acgccatcac ccgcgttgat ggggccgtgc
    14581 agttgaatga aagtctgtgc gtaagctgca aactgtgcgg catcgcctgc ccgtttggcg
    14641 caattgaatt ttccggcagc cgtccgctgg atattccggc aaacgccaat accccgaaag
    14701 cgccaccggc accgcctgct ccggcgtgtg tcagcacatt gcttgactgg gtgccaggta
    14761 ttcgcgcgat cgccgtcaaa tgtgaccttt gtagctttga tgaacaaggt ccggcctgtg
    14821 tgcggatgtg cccgactaaa gccctgcatc tggtggataa caccgatatc gcccgcgtca
    14881 gcaaacgtaa gcgtgagctg acttttaaca cggactttgg cgatctcacc ttgtttcagc
    14941 aggctcaaag tggagaggct aaatgagcgc aatttccctg atcaatagcg gcgtggcatg
    15001 gtttgtcgcc gccgctgttc tggcatttct cttttctttt caaaaagcgc taagtggctg
    15061 gatagccgga attggcggcg cggttggtag tctgtatacg gcagccgcgg gcttcactgt
    15121 actgactggc tcggttggcg tgagcggtgc gctgtcgctg gtaagctacg atgtgcaaat
    15181 ctccccgctt aacgcgattt ggctgattac cctcggtctt tgcggtctgt ttgtcagcct
    15241 ctataacatt gactggcatc gccacgcgca ggtgaagtgc aacggcttgc agatcaatat
    15301 gttgatggct gccgccgtct gcgccgtcat cgccagcaac ctcggcatgt tcgtggtaat
    15361 ggccgaagtc atggccttgt gtgcggtgtt cctcaccagc aacagcaaag agggcaaact
    15421 gtggtttgcg ctggggcgtc ttggcactct gctgctggtg attgcttgct ggctgctgtg
    15481 gcagcgttat ggcacgctgg atctgcgcct gctggatatg cgtatgcaac agctgccgct
    15541 cggttccgat atctggctgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
    15601 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
    15661 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
    15721 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
    15781 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
    15841 cctgctggct taccacaccc tggaaaatat cggcattatc ctgctggggc tggggctggg
    15901 gctggggctg gcgtaacggg tatcgcgctc gaacaaccgg cgctgattgc tcttggcctg
    15961 gtcggtggtc tgtaccatct gcttaaccat agcctgttca aaagcgtact gttcctcggg
    16021 gcggggagcg tctggttccg taccggtcat cgcgatatcg aaaaactcgg tggtattggc
    16081 aagaaaatgc cggttatctc catcgccatg ttagtcgggt tgatggcaat ggctgcgctg
    16141 ccgccactga acgggtttgc cggggaatgg gttatctatc aatccttctt caaactgagc
    16201 aatagcggcg cgtttgttgc ccgtctgctg gggccgctgc tcgctgtggg tctggcaatt
    16261 accggtgcgc tggcggtgat gtgtatggcg aaagtctatg gcgtcacgtt cctcggcgcg
    16321 ccgcgtacca aagaagccga aaacgccacc tgtgcgccgc tcctgatgag cgtaagcgta
    16381 gtggcactgg cgatttgctg cgtaattggc ggtgttgctg cgccgtggct gctgccgatg
    16441 ctctctgctg ctgtacctct gcctctggag cctgctaaca ccaccgtttc tcaaccaatg
    16501 atcacattgc tgctgattgc ctgcccgctg ctgccattca tcattatggc gatttgcaaa
    16561 ggcgaccgcc tgccgtcacg ttcccgcggt gcggcctggg tgtgcggcta cgaccatgaa
    16621 aaatcaatgg tgattaccgc tcacggtttt gccatgccgg ttaaacaggc gtttgcgcca
    16681 gtgctgaaac tgcgcaaatg gctgaatccg gtgtcgctgg tgccgggctg gcagtgcgaa
    16741 ggtagtgcgt tgctgttccg ccggatggcg ctggttgaac tggcggtact ggtggtgatt
    16801 attgtttcac gaggagcctg agaatgagtg ttttatatcc gttaattcag gcgctggtgt
    16861 tatttgccgt tgcgccgctg ctctccggta ttacccgcgt ggcgcgcgcc cgtctgcata
    16921 accgtcgcgg gccgggcgtg ttgcaggagt atcgcgacat tatcaaactg ctgggccgcc
    16981 agagcgttgg cccggatgcc tctggctgga tgttccgcct gacgccgtat gtgatggtgg
    17041 gcgtcatgct gactatcggt tctccgctgc cgcaactggg cgatttgatc accttactgt
    17101 atctcttcgc catcgcgcgt ttcttctttg ccatttccgg tctggatacc ggtagcccgt
    17161 ttaccgctat cggtgcgagc cgtgaagcga tgcttggcgt gctggtcgaa ccgatgttgc
    17221 tgcttggtct gtgggttgcc gcacaggttg ccggttccac caacatcagc aacattaccg
    17281 acaccgttta tcactggccg ctgagccaaa gcatcccgct ggtgctggcg ctctgcgcct
    17341 gtgcgttcgc cacttttatc gaaatgggca aactgccgtt cgacctggcg gaagccgagc
    17401 aggagttgca ggaaggtccg ctctccgaat acagcggtag cggctttggc gtcatgaaat
    17461 ggggtatcag cctgaaacag ctggtggtgt tgcagatgtt cgtcggggtg tttattccgt
    17521 ggggacaaat ggaaaccttc accgtcggtg gactgctgct ggcgctggtg attgccatcg
    17581 tcaaactggt ggtcggcgtc ctggttatcg cgctgttcga aaacagtatg gcccgtctgc
    17641 gtcttgatat tactccgcgc attacctggg ctgggtttgg ctttgcattt ttagcgttcg
    17701 tctccttgct ggcggcgtga ttaaagagag tttgagcatg tctgaagaaa aattaggtca
    17761 acattatctc gccgcgctga atgaggcatt tccaggcgtc gtgctggacc acgcctggca
    17821 gaccaaagat cagctgactg tcaccgtaaa ggtgaactat ctgccggaag tggtggagtt
    17881 tctctactac aagcagggtg gctggctgtc ggtgctgttt ggtaacgacg aacgcaaact
    17941 gaatggtcat tacgccgttt attacgtgct gtcgatggag aagggcacca agtgttgggt
    18001 aacggttcgc gtcgaagttg acgccaacaa accggagtat ccgtccgtga cgccgcgcgt
    18061 tccggcggcg gtgtggggcg agcgcgaagt gcgcgatatg tacggtttga ttccggttgg
    18121 tctgccggat gactggccgg atgaacttta tccgctgcgt aaagacagca tggattatcg
    18181 tcagcgtccg gcaccgacta ccgatgctga aacctacgag ttcatcaacg aactgggcga
    18241 caagaaaaac aacgtcgtgc cgattggtcc gctgcacgtc acttctgacg aaccgggtca
    18301 cttccgtctg ttcgtcgatg gcgaaaacat tatcgacgcc gactaccgtc tgttctacgt
    18361 ccatcgcggt atggaaaaac tggcggaaac ccgcatgggt tataacgaag tgaccttcct
    18421 ctctgaccgt gtgtgcggga tctgcggctt tgcccacagc accgcctaca ccacgtcggt
    18481 ggaaaacgcg atgggtattc aggtgccaga acgcgcgcag atgatccgcg ccattctgct
    18541 ggaggtggaa cgcctgcact cgcatctgct caacctcggc ctcgcctgtc actttaccgc
    18601 cttcgactcc ggctttatgc agttcttccg cgtgcgtgaa acctccatga aaatggcaga
    18661 gatccttacc ggtgcgcgta aaacctacgg cctgaacctg atcggcggga ttcgtcacga
    18721 tctgctgaaa gatgacatga tccagacccg ccagctggcg caacaaatgc gtcgtgaagt
    18781 gcaggagctg gtggatgtgc tgctgagtac gccgaacatg gaacaacgca ccgtcggcat
    18841 tggtcgtctg gacccggaaa tcgcccgcga tttcagtaac gttggcccga tggttcgcgc
    18901 cagcggtctc gcccgcgata ctcgcgccga tcacccgttt gtcggttatg gcctgctgcc
    18961 aatggaagtc cacagcgagc agggctgcga cgttatttcc cgtctgaaag tgcgaatcaa
    19021 cgaggtctat accgcgctga atatgatcga ctacggtctg gataacctgc cgggcggtcc
    19081 gctgatggtg gaaggcttta cctacattcc gcaccgtttc gcgctgggct ttgccgaagc
    19141 gccgcgcggc gacgatatcc actggagcat gaccggcgac aaccagaagc tgtaccgctg
    19201 gcgctgccgt gccgcgacct acgcgaactg gccgaccctg cgctacatgc tgcgtggcaa
    19261 taccgtttcc gatgcgccgc tgattatcgg tagcctggac ccttgctact cctgtaccga
    19321 ccgcatgacc gtagtcgatg tgcgtaagaa gaagagcaaa gtggtgccgt acaaagaact
    19381 cgagcgttac agcattgagc gtaaaaactc gccgctgaaa taaggaatcg ccatgtttac
    19441 ctttatcaaa aaagtcatca aaaccggcac ggcgacctcg tcttatccgc tggagccgat
    19501 tgcggttgat aaaaacttcc gtggtaagcc agagcagaac ccgcagcagt gcatcggctg
    19561 cgcggcttgc gtcaatgcct gcccgtcaaa cgccttaacg gttgaaactg acctcgccac
    19621 aggagagctg gcgtgggagt ttaatctcgg gcgctgcatc ttctgtggac gctgcgaaga
    19681 agtttgcccg acggcggcga tcaaactgtc gcaagagtac gaactggcgg tgtggaagaa
    19741 agaaaacttc ctgcaacagt cccgcttcgc gctgtgcaac tgccgcgtct gcaatcgtcc
    19801 tttcgccgta cagaaagaga tcgactacgc cattgcgctg cttaagcaca atggtgacag
    19861 ccgcgcggaa aaccaccgcg aaagctttga gacttgcccg gaatgtaagc gccagaaatg
    19921 cctggtgccg tccgaccgta ttgaactgac tcgccatatg aaagaggcca tctgatgagc
    19981 aatttattag gcccccgtga cgccaacggc attccggtcc cgatgacggt ggatgaatcc
    20041 atcgccagca tgaaggcgtc gttactgaaa aaaatcaaac gttctgccta tgtttaccgc
    20101 gtggactgcg gcggctgcaa cggctgcgaa atcgaaattt tcgccacgct ttctccgctg
    20161 ttcgatgcag aacgcttcgg cattaaagtc gttccttcac cgcgtcatgc ggatatttta
    20221 ctgtttaccg gcgcggtcac ccgtgcaatg cgatcccctg cgctgcgtgc gtggcagtcc
    20281 gcgccggacc cgaaaatctg tatctcatac ggtgcctgcg gtaacagcgg cgggatctta
    20341 cacgatctct actgcgtgtg gggcggtacg gataaaattg tccctgtgga tgtttatatc
    20401 cctggctgcc cgccaacgcc tgccgccacg ctgtacggct ttgcaatggc gctcggcctg
    20461 ctggagcaga aaattcacgc ccgtgggccg ggtgaactgg atgaacaacc ggcggagatc
    20521 ctgcatggcg atatggtgca gccgctgcgc gtgaaagtgg atcgcgaagc acgtcgcctg
    20581 gcgggttatc gttacggtcg tcagattgcc gatgattacc ttacacagtt agggcagggc
    20641 gaagaacagg ttgcacgctg gctggaagcg gaaaacgatc cgcgtctgaa cgagattgtc
    20701 agccatctga atcatgttgt tgaagaggcg cgtatccgat gagtgaaaag gtggtgttca
    20761 gtcaactgag ccgtaaattt attgatgaga acgatgccac gcccgccgag gcgcagcagg
    20821 tggtctatta cagcctggcg attggtcacc accttggggt tatcgattgc ctggaagcgg
    20881 cgctcacctg cccatgggat gaatatctgg catggattgc cactctggag gcgggcagcg
    20941 aagcccgcca caaaatggaa ggcgtaccga aatatggcga gatcgtcatc gacattaacc
    21001 atgtgccgat gctggccaac gcattcgata aagcccgggc agcgcaaact tcgcagcagc
    21061 aggaatggag tacaatgctg ttaagtatgc tgcatgatat tcatcaggaa aacgccatct
    21121 atttgatggt gaggagactg cgtgactgac gttttactct gtgttggcaa tagcatgatg
    21181 ggcgatgatg gcgctggccc gctgctggcg gaaaagtgcg ccgccgcgcc gaaaggtaac
    21241 tgggtggtga ttgacggcgg tagtgcaccg gaaaacgaca tcgtcgccat ccgtgaactg
    21301 cgcccgacac gactgctgat tgtcgacgcc acggatatgg ggctaaaccc cggcgaaatc
    21361 cgcatcatcg acccggatga tatcgccgag atgtttatga tgactaccca taacatgccg
    21421 ttgaattacc ttatcgacca gttaaaagaa gacattggtg aagtgatttt cctcggcatt
    21481 cagccggata tcgtcggctt ttactacccg atgacccagc cgattaaaga tgcggtagaa
    21541 accgtttatc aacgactgga aggctgggaa ggaaatggtg gatttgcgca gttagcggtg
    21601 gaagaagaat aattctccat cagggaattc agggaattca gggaattcag ggaattcagg
    21661 gaattgaaca tcagaacagg gatgtgcgct tttttattga gcttagtgct gcctgcacag
    21721 gccaccagct ttaccgaata tctgccaatg agtgacagcg aatacgcgca aaagcgggcg
    21781 ctgaaacctc tcctgaccat gccttatgat gccgagcaaa cctggcattt taggaaagtg
    21841 ggcgttgctg gcgtgacgct tgagaagatg ccaaatgaag aagattactg gcaattaacc
    21901 gctaaagacc gcgccggtaa atcctggact gtgcctgtgg gtatgctggc aaacatagcg
    21961 ggcaaagggc aactctatcg cgccgatctt gatcgtaacg gcattcagga tctggttatc
    22021 tggttaccaa gtaccggtaa tggtctggct ccttatgcac atttaattct tatgacgttc
    22081 acgcgcgaag gccgtccctg tgtctttgaa cccaggggat tctacaccgc aagcaaaact
    22141 ggcgtagatg atttgctgga tttgcaaggc aatggtcaca cgcaattgct ggatatgcag
    22201 tttaattctg gttactggat aaccagtctg tatcaggtaa aagacgccaa atggcagcgt
    22261 gttcacggct ggtttgacaa gttaagctat cccgcgctca cgcgttttac ttataccccc
    22321 aaccgtaaac tcgtcctcaa acccatcgcc gggcgcgacc cgcaaacgga agatctggcg
    22381 cagacgcagc gttgcttgat aaagggggat gtgcttgagg ggggaaccag aactgacttt
    22441 ttgcagagtg gctatgagga ttttgggggt aattatatcg cgcacaagtg tttacatatt
    22501 gtgaacttgg atttgatgag ctatagtttc cgaacacata aggatagaac tcgcagatgt
    22561 ggctgtttac ttactataag gaagttaaaa cttaaaaatg cacatcattt ctaaagcgcc
    22621 atttgaagag tctgcgcgta aatatcctaa cgacgctctg gctcttcagg cattgtatcg
    22681 ggttattaag gaaacggatt tttctacgcc agaagagatg cgaacggcgt ttcccaacct
    22741 cgacaatttt aaatatcgca acaaatggta cgttctggat gtaggtggga acaacctagg
    22801 ggtgattgcc tacataaact ttgttaataa gcggttcttt gtgaagcaca taactaatca
    22861 cgccgagtat gacaaactga cacgttacta cagagagaac aaagaatgac tgctaatgct
    22921 gcacacgctg ttaaagcaac aagagaactg gtcaatgcag tcccctttct gggaggtagt
    22981 gactcagaag atgattatcg tgaagcactg gaactggtcg aatatttgat tgaagaagat
    23041 gacaccaatc cacttatcga ctttctggcg agccgcattg cagaatatga aaacaacaac
    23101 caagagtttg cagagttcga caaagccgtt gcggcaatgc ctgttggcgt ggcgttactt
    23161 cgcacgctga ttgatcaaca taatctgacc tatgcggatt tgaagaacga aatcggctct
    23221 aaatccctgg tcagtcagat tttgtctgga caacgatccc tgacgatatc ccacattaag
    23281 gcactttcag cccgatttgg cgttaagccg gaatggtttc tttgattttt gactaatgcc
    23341 aaatgctaaa ttgcccgatg cgctgcgctt atcgggccta catgtcgcac gaactatgta
    23401 ggccggataa ggcgttcacg ccgcatccgg cactgttact tactctaaat cttccccatt
    23461 actggcaatc acttttttat accaccagaa cgatttctta cgtgtgcgcg tcagcgtgcc
    23521 gttgcctgcg tcgtcacggt ctacgtagac aaaaccgtag cgtttgctca tttcacccgt
    23581 agaggcggaa actaaatcaa tacagcccca tgtggtgtag cccatcagcg gaatgccatc
    23641 tgcaatcgct tcgcctattg cgcggatatg ttcgcgtaaa tagctgatgc gatagtcatc
    23701 gttaatctcg ccattggcag caagttcatc ttttgcgccg aggccgtttt ccaccagaaa
    23761 cagcggcttc tgataacgat cgtacatcat attcatggtg atacgcagac cgagtggatc
    23821 aattccccag ccccagtcgc tcacttgcag atacgggtta cgcagcgatt tcaccacgtt
    23881 cgccgcactg ctgttgttgg cgttcatctc cgccgaggcg cagcgcgagg cgtaatagct
    23941 gaaagagaca aaatcgacgg tgtttttcag gatctcatca tcgcccagcg ctttatctat
    24001 ggttacccct ttttcgcgaa atacgcgggc agagtaagcc ggatacgcgc cgcgcgcctg
    24061 cacatcgata aaaaacaggt tttcccgatc tttctccagc gccgcccaga catcttccgg
    24121 tttgcagctg taagggtaga agttaccgcc cgccagcata cagcccacct gattttgcgg
    24181 gttaacctca tgggcgattt tggtggctag cgcactggca accagctggt gatgcgcggc
    24241 ctgatatttc acctgatcct gattttcacc ttcttcaaac accagacccg cgccggagaa
    24301 cgggctatgc aacataatgt tgatttcatt gaaggttagc cagtatttca ccagaccatc
    24361 aaatgcttca aagcaggttc tggcgtagcg gctgaaaaac tccaccagct tgcggttacg
    24421 ccaggagcca tattcggtga ccagatgcat cggcacatcg aagtggcaca acgtgaccag
    24481 cggttcgata ccgtactttt tacactcttc aaagacagga cgataaaaag caatgccctg
    24541 ctgattgggc gtgatttcat cgtcctgcgg aaagagacgg ctccaggcaa ttgaggtacg
    24601 gaaaaccttg aatcccatct ctgccatcag ggcgatatct tctttataac gatgataaaa
    24661 atccgtcgcc tcatggctgg gataaaactc gtcatcgcgc aactgaaaac gtttttccag
    24721 ccccagtttc accgccattc gatgctcgcc gtgtgggatc atatcgacgg tggtcagccc
    24781 ttttccgcct tcacggaacg caccttcaga ctggttggcg gcaagcgcgc cgccccataa
    24841 aaaaccttct ggaaatactg acattttcat cctcaattaa gacttacttc tttggcgacg
    24901 gtcggttgat gctttcgcgc ctgggcagcc gcttcctcaa caggaatatc ctcaaagccg
    24961 agcaacagtg tgaggataaa cgacagcacc accgccagcg ccatgaccgc gaacacccag
    25021 acgatgctca ttggattcgc cggatcgaag aattgcacgc tggtaaatag ccccggcgct
    25081 gccattgagt ggctggcaag ccccgccata ccggcaaccg cgccgcaaat aaaaccgctg
    25141 ataagactgg cgataagcgg acgtttcagg cggatcgcca cgccgtataa cgccggttcg
    25201 gaaatccccg ccataatggc tgatgccgcc gcagccagcg ccgtctggcg cagttccggg
    25261 tttttcgttt tccacggcac cgccagtgat gaaccgccca gcgacaggtt agcgccgatc
    25321 tctgatggca tgaccattcc ttctttgccg gtttcggcaa tggtctgaat gatggttggc
    25381 gtaaagacgc ggtgcatccc ggtcattacc agcagaggcc acagcgcgcc cataatggcg
    25441 actgaaagcc agcccagata accatgaatg gtgtaaacca gcgccgaaat ggcgctaccg
    25501 atccagatac caatcgggcc aatcagcagg attgccagcg gtgcggcaat caacacaatc
    25561 agcattggct tgaggaagtt tttggtcacc gccggtgtaa tgcgatccac ccagcgttcg
    25621 atatatgaca ggcaccaggt catcaccagc gccgggatca ccgtgtaggt gtatttcacc
    25681 gcggtgaccg ggatcagggc aaattcgaca tgttcaccct gggccgcttt cgccattaat
    25741 tcaataaaac tcggatgtac cagcacaccc gcgatggcaa tcgccagcga catattggtt
    25801 ttaaatttga tggcggcaga tgccgcgacc attagcggca ggaagaagaa agcaccgtca
    25861 ccaatcacat tcagaatggt taaggtcggc gatccttttg tcagcacgcc gctcatctcg
    25921 aggatcatcg ccagcagttt gaccatcgat ccgccgataa tcgccgggat cagcggtgac
    25981 atggtgccga tcagcgcatc gaggatcccc gcaccaatgc gacgcagcgt gagtttgggt
    26041 ttacccacgg gctgtgcggg ctgcatatct cccggcagca ggctgacgac ttcctgaaag
    26101 gcctgcgaaa ccgtattgcc gataattacc tgacactggt tgtcactacg taccacgccg
    26161 agcacgccgg ggatggtttt taacgtcggg ctgtcgataa gctggtcatc tttgataaca
    26221 aagcgcaaac gcgtcataca gtgcgtgact gccgagatgt tatcaacgcc gcccagtgcc
    26281 gctatcaccg agcgtgccag cgccgcataa tttttggcca tcggatttta tcctgttatc
    26341 agtagggtag atgtagttgt tatcagtcga cgtgacggaa aaataccgat gctgaaacgg
    26401 aattaagaaa ccggtttcac aaaatcatga atagttgtca ctaaataagc aagagagtta
    26461 attgttcatt ttttatattt gtgaaaccgg tcacctgaat atttttcccg ccgattgcgc
    26521 aaggacaatc ggcaaacggt gcagtacact gcgttgattg cgtgtaaagg gatgaaaatg
    26581 atgacgacga tgctggaagt ggcgaagcgc gccggggttt caaaagcgac cgtttcccgc
    26641 gtgctttcag gtaatggcta cgtcagccag gagactaaag atcgcgtgtt tcaggcggta
    26701 gaagagagca gttaccgccc aaatttgctg gcccgcaatc tgtcggcgaa gagtactcag
    26761 acgctggggc tggtagtgac caacacgctt tatcacggca tttattttag tgaattactg
    26821 tttcatgccg cgcgaatggc ggaagagaaa gggcgacagt tgctattggc agatggtaaa
    26881 cacagcgccg aagaagagcg ccaggcgatt cagtatctgc tggatttgcg ctgcgacgcc
    26941 attatgattt acccgcgttt tttaagcgtg gatgagattg atgacatcat tgacgcgcac
    27001 agtcagccga taatggtgct taatcgccgc ctgcgcaata atagcagcca tagcgtctgg
    27061 tgcgatcata aacagaccag ctttaacgcc gtggcagagt tgataaacgc cgggcatcag
    27121 gagattgctt tccttaccgg ctcgatggat tcccccacca gcattgaacg tcttgccggg
    27181 tataaagacg cgctgtcgca gcatggtatt gcgctcaatg aaaaacttat cgctaacggt
    27241 aaatggacgc ccgccagcgg ggccgaaggg gtagaaacgt tgctcgaacg tggggctaaa
    27301 tttagcgcgt tagttgccag taacgacgat atggcgatag gtgcgatcaa agcgttgcac
    27361 gagcgcggcg tagcggtgcc agagcaggtg tcagttatcg gattcgatga tatcgcaatt
    27421 gccccctata tcgttccggc gctctccagc gtaaaaattc cggtgactga gatgattcag
    27481 gaaattattg gacggctgat ttttatgctc gatggcgggg atttctcacc gcctaaaacc
    27541 tttagcggaa aactgatccg ccgcgactca ctcattgccc tttcgcgata aacattgtca
    27601 tcacatgtca tcgtcactag tgtcgatgac atcttttttc ctgcattaca gaaattaatt
    27661 tccttaaata acagcaaatt aaaaactggc atgatttgtg aatgtatcgg cgc
//
LOCUS       DAJTNM010000042        27459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTNM010000042 DAJTNM010000000
VERSION     DAJTNM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.042"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO172_001919"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO172_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5512119.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO172_001920"
     CDS             complement(1614..1937)
                     /locus_tag="OO172_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5512120.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO172_001921"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO172_001921"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5512121.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO172_001922"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO172_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5512122.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLHDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO172_001923"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO172_001923"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5512123.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO172_001924"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO172_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5512124.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO172_001925"
     CDS             7537..8574
                     /locus_tag="OO172_001925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5512125.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO172_001926"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO172_001926"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5512126.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO172_001927"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO172_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5512127.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO172_001928"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO172_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5512128.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO172_001929"
     CDS             10745..11626
                     /locus_tag="OO172_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5512129.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO172_001930"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO172_001930"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5512130.1"
                     /translation="MTVTTFSELELDKSLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO172_001931"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO172_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5512131.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO172_001932"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO172_001932"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR5512132.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO172_001933"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO172_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5512133.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO172_001934"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO172_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5512134.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO172_001935"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO172_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5512135.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO172_001936"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO172_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5512136.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTLTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO172_001937"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO172_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5512137.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO172_001938"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO172_001938"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5512138.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO172_001939"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO172_001939"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5512139.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO172_001940"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO172_001940"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5512140.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO172_001941"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO172_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5512141.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO172_001942"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO172_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5512142.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO172_001943"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO172_001943"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5512143.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO172_001944"
     CDS             24893..25273
                     /locus_tag="OO172_001944"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5512144.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO172_001945"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO172_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5512145.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO172_001946"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO172_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5512146.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..>27459
                     /locus_tag="OO172_001947"
     CDS             26442..>27459
                     /locus_tag="OO172_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR5512147.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFSQ"
BASE COUNT         6769 a         6838 c         7202 g         6650 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tatttaccga aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgtgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattaa aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggcttttg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggaaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatctact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatgc tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagttcc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgctaac ttggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgaggg cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtacgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcttg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatac aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac gactatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cattaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttcacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggagtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgacct caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagcaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttccttc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg
    27181 ttgctgcctg cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg
    27241 ttcgtccgat taatgtccat aacctgcgct gggactacgt ttacgaggag gctggtgaaa
    27301 ttgtttatcc ggaaggggtt atcggcatgc gaggggctat gaaaacacaa aaggctttcc
    27361 gcctgccgat aacgcctgag atccggcgga ttatcgacga gcagaaagcc tggcgtgatt
    27421 cagttcctga gtgcaacagg gattatgtat tttcgcagc
//
LOCUS       DAJTNM010000043        27126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTNM010000043 DAJTNM010000000
VERSION     DAJTNM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.043"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..437
                     /locus_tag="OO172_001948"
     CDS             <1..437
                     /locus_tag="OO172_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR5512148.1"
                     /translation="IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQV
                     ELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWF
                     TVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="OO172_001949"
     CDS             complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="OO172_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5512149.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1230..2252
                     /gene="pldB"
                     /locus_tag="OO172_001950"
     CDS             1230..2252
                     /gene="pldB"
                     /locus_tag="OO172_001950"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5512150.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2260..3060
                     /gene="yigL"
                     /locus_tag="OO172_001951"
     CDS             2260..3060
                     /gene="yigL"
                     /locus_tag="OO172_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5512151.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3136..4035
                     /gene="bioP"
                     /locus_tag="OO172_001952"
     CDS             3136..4035
                     /gene="bioP"
                     /locus_tag="OO172_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5512152.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3923..4876)
                     /gene="metR"
                     /locus_tag="OO172_001953"
     CDS             complement(3923..4876)
                     /gene="metR"
                     /locus_tag="OO172_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5512153.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5112..7373
                     /gene="metE"
                     /locus_tag="OO172_001954"
     CDS             5112..7373
                     /gene="metE"
                     /locus_tag="OO172_001954"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5512154.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7412..8227)
                     /locus_tag="OO172_001955"
     CDS             complement(7412..8227)
                     /locus_tag="OO172_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5512155.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8489..9250
                     /gene="udp"
                     /locus_tag="OO172_001956"
     CDS             8489..9250
                     /gene="udp"
                     /locus_tag="OO172_001956"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5512156.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9391..10818
                     /gene="rmuC"
                     /locus_tag="OO172_001957"
     CDS             9391..10818
                     /gene="rmuC"
                     /locus_tag="OO172_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5512157.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFCRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSC"
     gene            10913..11668
                     /gene="ubiE"
                     /locus_tag="OO172_001958"
     CDS             10913..11668
                     /gene="ubiE"
                     /locus_tag="OO172_001958"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5512158.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11682..12287
                     /gene="ubiJ"
                     /locus_tag="OO172_001959"
     CDS             11682..12287
                     /gene="ubiJ"
                     /locus_tag="OO172_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5512159.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGIRKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12284..13924
                     /gene="ubiB"
                     /locus_tag="OO172_001960"
     CDS             12284..13924
                     /gene="ubiB"
                     /locus_tag="OO172_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5512160.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            14003..14272
                     /gene="tatA"
                     /locus_tag="OO172_001961"
     CDS             14003..14272
                     /gene="tatA"
                     /locus_tag="OO172_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5512161.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTISDKQADTNQEQAKTEDAKRHDKEQV"
     gene            14276..14791
                     /gene="tatB"
                     /locus_tag="OO172_001962"
     CDS             14276..14791
                     /gene="tatB"
                     /locus_tag="OO172_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5512162.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14794..15570
                     /gene="tatC"
                     /locus_tag="OO172_001963"
     CDS             14794..15570
                     /gene="tatC"
                     /locus_tag="OO172_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5512163.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15612..16394
                     /gene="tatD"
                     /locus_tag="OO172_001964"
     CDS             15612..16394
                     /gene="tatD"
                     /locus_tag="OO172_001964"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5512164.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="OO172_001965"
     CDS             complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="OO172_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5512165.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17046..18539
                     /gene="ubiD"
                     /locus_tag="OO172_001966"
     CDS             17046..18539
                     /gene="ubiD"
                     /locus_tag="OO172_001966"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5512166.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18585..19286
                     /gene="fre"
                     /locus_tag="OO172_001967"
     CDS             18585..19286
                     /gene="fre"
                     /locus_tag="OO172_001967"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5512167.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="OO172_001968"
     CDS             complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="OO172_001968"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5512168.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="OO172_001969"
     CDS             complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="OO172_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5512169.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22931..24262
                     /gene="pepQ"
                     /locus_tag="OO172_001970"
     CDS             22931..24262
                     /gene="pepQ"
                     /locus_tag="OO172_001970"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5512170.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24262..24876
                     /locus_tag="OO172_001971"
     CDS             24262..24876
                     /locus_tag="OO172_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5512171.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24915..26366
                     /gene="trkH"
                     /locus_tag="OO172_001972"
     CDS             24915..26366
                     /gene="trkH"
                     /locus_tag="OO172_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5512172.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26378..26923
                     /gene="hemG"
                     /locus_tag="OO172_001973"
     CDS             26378..26923
                     /gene="hemG"
                     /locus_tag="OO172_001973"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5512173.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6230 a         6903 c         7469 g         6524 t
ORIGIN      
        1 tgattatcga aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc
       61 tctgctggat gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc
      121 ctgcaccaca ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca
      181 atctcgccaa tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg
      241 ataacgttgg cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg
      301 cgtggtttac cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac
      361 gtctggcgaa gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt
      421 tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
      481 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
      541 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
      601 agcaatcctt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
      661 ggtgacgccg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
      721 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
      781 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc
      841 ggcgcgccac tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac
      901 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
      961 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
     1021 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     1081 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     1141 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat
     1201 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     1261 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     1321 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     1381 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg
     1441 cggaactggc ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     1501 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     1561 atgattatgt tgatgatctg gcggcattct ggcagcagga ggttcagccc ggtccgtggc
     1621 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     1681 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     1741 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     1801 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     1861 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     1921 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     1981 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac
     2041 gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     2101 ctgtcgaagg aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa
     2161 aggacgcaat gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact
     2221 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     2281 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact
     2341 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     2401 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt
     2461 gcgcgcgttc acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatt
     2521 gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc
     2581 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     2641 gtgttcaaat atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg
     2701 tttttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc
     2761 tggggcgatc gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc
     2821 ggcgtgtcga aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag
     2881 gattgtattg cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa
     2941 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt
     3001 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     3061 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     3121 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     3181 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     3241 gttggcttgg cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa
     3301 acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     3361 ttccgcgctt atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg
     3421 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
     3481 tttagcgcct tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
     3541 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     3601 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     3661 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     3721 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     3781 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
     3841 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
     3901 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
     3961 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
     4021 gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg
     4081 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct
     4141 caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt
     4201 gaatcaataa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa
     4261 gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc
     4321 tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca
     4381 ccaggcgtac ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat
     4441 cggaggtcat caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca
     4501 cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg
     4561 gtgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg
     4621 gttcattgca ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca
     4681 acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga
     4741 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca
     4801 acgttgccgc agcggctgcg agcgagccgc agttccgcaa cgcttgtagc gttttcaggt
     4861 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
     4921 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt
     4981 aaatttatgg tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg
     5041 gcgatgaata aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta
     5101 gaggaagaaa aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
     5161 gcgagctgaa aaaagtgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
     5221 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
     5281 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc
     5341 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
     5401 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
     5461 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
     5521 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg agccacaacg
     5581 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
     5641 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
     5701 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
     5761 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
     5821 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
     5881 tgcctgttca ggggctgcat gtggaccttg tacatggtaa agatgacgtt gttgaactgc
     5941 acaagcgcct gccttctgac tggctgctgt ctgcgggtct aatcaatggt cgtaacgtct
     6001 ggcgcgccga tcttactgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
     6061 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
     6121 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
     6181 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
     6241 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
     6301 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
     6361 agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
     6421 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact
     6481 accgcacggg cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac
     6541 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
     6601 acctcgacgg atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
     6661 tgaagccacc gattgtcatt ggtgacatta gccgcccggc accgattacc gtggagtggg
     6721 cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg gatgctgacg gggccggtga
     6781 ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga
     6841 ttgcgctggc gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc
     6901 agattgacga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt
     6961 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
     7021 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc
     7081 tggacgcaga cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt
     7141 ttgaagagtt tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa
     7201 acgtaccgag cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
     7261 cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
     7321 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt gcgtcggggg taaatccaaa
     7381 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
     7441 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
     7501 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
     7561 tagcagcccg cagcgcctgg cgcatcgttt caacgctctc ctgcggaatg ctgttatcct
     7621 gaccaccata taagccgaga accggtgcgt taagatcggt tgcgatatca acaggttgtt
     7681 tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta
     7741 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
     7801 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
     7861 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
     7921 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
     7981 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
     8041 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
     8101 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
     8161 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
     8221 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
     8281 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
     8341 tgatttatgt gatatgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
     8401 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
     8461 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
     8521 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
     8581 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
     8641 tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
     8701 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
     8761 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
     8821 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
     8881 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac
     8941 tcacgttggc gtgacagttt cttctgatac cttctaccca ggtcaggaac gttacgatac
     9001 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
     9061 cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gtcagggcct
     9121 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
     9181 tgagacgatg aaacaaactg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg
     9241 tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
     9301 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
     9361 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
     9421 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
     9481 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
     9541 caaattaccc aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc
     9601 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
     9661 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
     9721 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
     9781 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
     9841 ggtttctgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
     9901 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
     9961 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
    10021 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    10081 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga
    10141 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc
    10201 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    10261 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    10321 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    10381 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    10441 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgtaa cgcccagcaa
    10501 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    10561 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
    10621 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    10681 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    10741 cgacttcggt cggttccggg gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    10801 aatcagcagt cgtgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    10861 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    10921 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    10981 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    11041 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    11101 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    11161 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    11221 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    11281 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    11341 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    11401 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    11461 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    11521 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    11581 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    11641 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    11701 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
    11761 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    11821 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    11881 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    11941 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    12001 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
    12061 ccggtgatat cgccgctgaa ggaatcagaa aagccatgcg cggaggcgca aagttcctgc
    12121 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    12181 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    12241 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc ctggtgaagt
    12301 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    12361 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    12421 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
    12481 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
    12541 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
    12601 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    12661 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    12721 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    12781 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    12841 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    12901 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
    12961 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    13021 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    13081 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    13141 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    13201 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    13261 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    13321 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    13381 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    13441 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    13501 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    13561 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgccct
    13621 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    13681 actggtttac gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc
    13741 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    13801 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    13861 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    13921 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    13981 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    14041 cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    14101 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    14161 cagtcaggat gctgatttta ctgcgaaaac tatctccgat aagcaggcgg atacgaatca
    14221 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    14281 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    14341 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    14401 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    14461 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    14521 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    14581 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    14641 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    14701 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc
    14761 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    14821 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    14881 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
    14941 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    15001 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    15061 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    15121 ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc
    15181 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
    15241 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
    15301 gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    15361 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
    15421 ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt
    15481 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    15541 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    15601 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    15661 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    15721 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    15781 caacggcggg cgttcatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    15841 ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    15901 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
    15961 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    16021 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    16081 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    16141 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    16201 aattgctgat cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    16261 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    16321 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    16381 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    16441 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    16501 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa gggggggccg
    16561 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    16621 tcgctggcga cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg ctgatggtcg
    16681 tggtatgaat cacttctgga tcaaattcca caaacaggta gttagggaat aacggttcac
    16741 tgactgcggt acgtttaccc cgtacgattt tttccagggt gatcatcgga gcgaggcaat
    16801 taaccgcctg tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    16861 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    16921 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    16981 atttaacaaa tttacagcat tgcaaagatg aacgccgtat aatgggcgca gattaagagg
    17041 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc
    17101 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    17161 ctgaccgcac tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    17221 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    17281 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    17341 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caggtgttaa
    17401 acatgccgac aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    17461 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    17521 ttacctgggg gctgaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    17581 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    17641 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    17701 tggcgctggg tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc
    17761 tttcagagta tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta gtaaagtgta
    17821 tttccaatga cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg tatatcgatc
    17881 ctggtgagat ggcgccggaa gggccgtatg gtgaccacac aggttactat aatgaagtcg
    17941 atagtttccc ggtgtttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt
    18001 ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag
    18061 tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    18121 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    18181 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    18241 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    18301 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    18361 attttgcctc gcctgtctct gggctgggtt caaaaatggg gctggatgcc acgaataaat
    18421 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtca
    18481 cgtatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    18541 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    18601 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    18661 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    18721 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    18781 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    18841 aaatcgtggt cgacattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    18901 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    18961 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    19021 tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc
    19081 cggtggttga acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg
    19141 tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    19201 tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    19261 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    19321 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaagtggtt
    19381 aaacccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac
    19441 caaactgaac gtctttgcgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg
    19501 aacaacccag cggatgaccc agcgcgatcg caccaccatt gaggttgatc ttctcgtcaa
    19561 tctgctccat tagcccgaga tctttaatgc acggcaggat ctgcgcggca aaggcttcgt
    19621 tcatctcaaa cacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg
    19681 aggccggaac cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac
    19741 gcacacgagc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    19801 tggcagctgc gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg
    19861 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    19921 cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt
    19981 catttttaaa tgcggccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    20041 aggcatcctg catttcacgg ctgataccgt gtatacgcgc cagcatttct gccgttaagc
    20101 ccatcatgcc cgccgctttg gctacattgc ggctcaggcc ggggtgaaaa tcgacgccgt
    20161 gactcatcgg cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc
    20221 cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    20281 tgacggtaac cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga
    20341 tgttaaaacc ctgctccagc gtctgttgca cacaacccca gtaaatatcg tcgagggccg
    20401 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    20461 ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    20521 cgacaatgac aacctgttcc attgtgattc cttaagccgt tttcaggtcg ccaaccggac
    20581 gggctggctc aaccggagga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    20641 ccggcacttc atacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg
    20701 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc
    20761 cgtagaccag cgccatatcc gcttccgccg gagtggcgat aatgccctct tccagacagc
    20821 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    20881 cgcgcttcgg ttggctcact tctgccagca ggtcttcaac ggcggcgtct tcttctttct
    20941 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    21001 agcggttggc atcaaacagc gcgtcgatgg catcgcggta atctttctgc atccgctgcg
    21061 ggaagcctgc tgccatgaca gcctgagcgt gatgcgcggt atcaatgccc acaacgtcca
    21121 gcagatatgc cgggcccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    21181 ggaaatccgc gccgtcgcgc agcagctggc tgaaaccggc gaaatacggg aacagcacgc
    21241 ggttaacaaa gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg
    21301 cccaggcgac aactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcta
    21361 ccaacggcat tcggtggacc gggttaaaga agtgcatccc gcagaagttt tccgggcgtt
    21421 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    21481 cctggcgtac tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcaa
    21541 caaccgcttc taccacaata tccacgcggt caaatccggc gtagtcgagc gttgggtgga
    21601 tcgtggagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    21661 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    21721 tgacaaccgg cacgcctttc cacgcggact ggtaagcgat accgccaccc atgatgcctg
    21781 cgcccagcac agcagcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    21841 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg
    21901 ccagcgggac aaaacttttg ttttccagat ttaaggcttc ttcacgacca aaacgggccg
    21961 cagcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgcg
    22021 cgaccattcc tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagcg
    22081 gttccagctt cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta
    22141 aaatcgccat cgccccttca atcagttttt ctgctttgac tacgccatcc accagaccga
    22201 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc agtgatttcc agtgcactgt
    22261 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    22321 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    22381 cgcattcgca gccaccaccc agcgcatagc cgttgacggc agcaatggtc ggcaccggca
    22441 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    22501 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    22561 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    22621 cgccgaggct ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    22681 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    22741 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcgg agtgtgtgtt
    22801 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    22861 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    22921 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga
    22981 acggactcgc gatgcgctgg cgcgcttcaa gctggatgca ttacttattc actccggcga
    23041 gctgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    23101 agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa
    23161 gccgaaactg tggttctatc tgccggttga ttactggcac aacgtcgaac cgctgccgac
    23221 ctccttctgg actgaagatg tggaagtgat cacgctgccg aaagccgatg gcattggtag
    23281 cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct
    23341 gcaactgggt attgaggcca gcaacatcaa cccgaaaggg gtgatcgact acctgcatta
    23401 ttaccgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    23461 ggtcaatggt catcgtgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    23521 caatattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt acagcaacat
    23581 tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    23641 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    23701 cctgacccgt acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt
    23761 aaatgatgaa caactggggc tgatcgccac catgaaagca ggcgtcagct atgtggatta
    23821 ccacatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    23881 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    23941 tggccatccg ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg
    24001 tacacacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    24061 gggcatggtg ttaaccatcg aaccgggtat ctacttcatt gaatcgcttc tggcaccgtg
    24121 gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    24181 cggcggcatt cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac
    24241 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    24301 gttgaagaga tcaaaaagag ccgcttcata acgatgttgg cgcataccga tggcgttgag
    24361 gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc
    24421 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    24481 gagccggcgg gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg
    24541 gaaattaccg ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    24601 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    24661 acgccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    24721 ttgctggggc agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg
    24781 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    24841 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    24901 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    24961 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    25021 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    25081 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    25141 tttggaccgt gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    25201 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta
    25261 cgctggtggg gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat
    25321 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    25381 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    25441 cgcgaattgc ggaaacggcg aaaactctgt ggttgattta tgtcttgctg accgtcgcct
    25501 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    25561 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagtc
    25621 cggctattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    25681 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    25741 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    25801 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    25861 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    25921 tgcttttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    25981 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    26041 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    26101 ccgtttgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    26161 tcgccacggg cgtggatgat ttttctgcct ttgcctcagt tgttgcgaca ttgaataacc
    26221 tggggccagg gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    26281 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    26341 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    26401 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    26461 ggggatccag acggatgtcg ccaatgtgca ccgcattgaa gaaccacaat gggaaaacta
    26521 tgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    26581 atttgtcaaa aaacatgcga cgcgactgaa ttcgatgccg agcgcctttt attccgtaaa
    26641 cctggtggcg cgcaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    26701 tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta
    26761 cccacgttat cgctggtacg accgttttat gctcaagctg attatgaaga tgtcaggcgg
    26821 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    26881 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa
    26941 tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat
    27001 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
    27061 gaattccgac agagaaagca aaaataaatg cttgactctg tagcgggaaa gcgtattatg
    27121 cacacc
//
LOCUS       DAJTNM010000044        26047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTNM010000044 DAJTNM010000000
VERSION     DAJTNM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.044"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            164..1147
                     /locus_tag="OO172_001974"
     CDS             164..1147
                     /locus_tag="OO172_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5512174.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1170..2681
                     /locus_tag="OO172_001975"
     CDS             1170..2681
                     /locus_tag="OO172_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5512175.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2706..3704
                     /locus_tag="OO172_001976"
     CDS             2706..3704
                     /locus_tag="OO172_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5512176.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3737..4831
                     /locus_tag="OO172_001977"
     CDS             3737..4831
                     /locus_tag="OO172_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5512177.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGYRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4843..5715
                     /locus_tag="OO172_001978"
     CDS             4843..5715
                     /locus_tag="OO172_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5512178.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5763..6185)
                     /locus_tag="OO172_001979"
     CDS             complement(5763..6185)
                     /locus_tag="OO172_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5512179.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6282..7484)
                     /locus_tag="OO172_001980"
                     /pseudo
     CDS             complement(6282..7484)
                     /locus_tag="OO172_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7494..8306)
                     /gene="hcaB"
                     /locus_tag="OO172_001981"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(7494..8306)
                     /gene="hcaB"
                     /locus_tag="OO172_001981"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5512180.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8303..8623)
                     /locus_tag="OO172_001982"
     CDS             complement(8303..8623)
                     /locus_tag="OO172_001982"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5512181.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8623..9141)
                     /locus_tag="OO172_001983"
     CDS             complement(8623..9141)
                     /locus_tag="OO172_001983"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5512182.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9138..10499)
                     /locus_tag="OO172_001984"
     CDS             complement(9138..10499)
                     /locus_tag="OO172_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5512183.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10635..11525
                     /gene="hcaR"
                     /locus_tag="OO172_001985"
     CDS             10635..11525
                     /gene="hcaR"
                     /locus_tag="OO172_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5512184.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11685..12824
                     /locus_tag="OO172_001986"
     CDS             11685..12824
                     /locus_tag="OO172_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5512185.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12816..14096)
                     /gene="csiE"
                     /locus_tag="OO172_001987"
     CDS             complement(12816..14096)
                     /gene="csiE"
                     /locus_tag="OO172_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5512186.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRITRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTPQQEQIRKMLESA"
     gene            complement(14287..15141)
                     /locus_tag="OO172_001988"
     CDS             complement(14287..15141)
                     /locus_tag="OO172_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5512187.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMLPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15286..16089)
                     /gene="suhB"
                     /locus_tag="OO172_001989"
     CDS             complement(15286..16089)
                     /gene="suhB"
                     /locus_tag="OO172_001989"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5512188.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGDL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16208..16948
                     /gene="trmJ"
                     /locus_tag="OO172_001990"
     CDS             16208..16948
                     /gene="trmJ"
                     /locus_tag="OO172_001990"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5512189.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17218..17706
                     /gene="iscR"
                     /locus_tag="OO172_001991"
     CDS             17218..17706
                     /gene="iscR"
                     /locus_tag="OO172_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5512190.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17818..19032
                     /gene="iscS"
                     /locus_tag="OO172_001992"
     CDS             17818..19032
                     /gene="iscS"
                     /locus_tag="OO172_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5512191.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19060..19446
                     /gene="iscU"
                     /locus_tag="OO172_001993"
     CDS             19060..19446
                     /gene="iscU"
                     /locus_tag="OO172_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5512192.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19463..19786
                     /gene="iscA"
                     /locus_tag="OO172_001994"
     CDS             19463..19786
                     /gene="iscA"
                     /locus_tag="OO172_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5512193.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19882..20397
                     /gene="hscB"
                     /locus_tag="OO172_001995"
     CDS             19882..20397
                     /gene="hscB"
                     /locus_tag="OO172_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5512194.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20414..22264
                     /gene="hscA"
                     /locus_tag="OO172_001996"
     CDS             20414..22264
                     /gene="hscA"
                     /locus_tag="OO172_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5512195.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22266..22601
                     /gene="fdx"
                     /locus_tag="OO172_001997"
     CDS             22266..22601
                     /gene="fdx"
                     /locus_tag="OO172_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5512196.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22613..22813
                     /gene="iscX"
                     /locus_tag="OO172_001998"
     CDS             22613..22813
                     /gene="iscX"
                     /locus_tag="OO172_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5512197.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22872..24155
                     /gene="pepB"
                     /locus_tag="OO172_001999"
     CDS             22872..24155
                     /gene="pepB"
                     /locus_tag="OO172_001999"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5512198.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24297..25073
                     /gene="sseB"
                     /locus_tag="OO172_002000"
     CDS             24297..25073
                     /gene="sseB"
                     /locus_tag="OO172_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5512199.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDCALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25568..>26047)
                     /gene="sseA"
                     /locus_tag="OO172_002001"
     CDS             complement(25568..>26047)
                     /gene="sseA"
                     /locus_tag="OO172_002001"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR5512200.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT         6169 a         6517 c         7222 g         6139 t
ORIGIN      
        1 caagcacctt aaaatcacca tcatacacta aatcagtaag ttggcagcat taccctacgt
       61 tcactaaacc ctattaactg taatatttga gcggcacgcg ctgccgctca tcacaaaata
      121 attagaataa tccgttgtta ataaaaaccc tacaggaggc actatgccta aaaaaatgag
      181 aacaacccgt aatctattat taatggctac gctattaggt agcgcactat ttgccagggc
      241 tgcggataaa gaaatgacca ttggtgcaat ataccttgat acccagggat attacgctgg
      301 agtgcgtcag ggcgttcagg atgcggcaaa agattcttca gtacaggtac agttaattga
      361 aactaacgcc cagggggata tttcgaaaga aagtaccttt gttgataccc tcgtggcgcg
      421 taatgtcgat gccattattt tatcggcagt gtctgaaaat ggcagtagcc gtaccgttcg
      481 tcgcgccagt gaagcgggta ttccggtgat ttgctacaac acctgtatta atcaaaaggg
      541 tgtcgataaa tatgtctcgg cgtatctggt cggcgatcca ctggaatttg gtaaaaaact
      601 gggtaacgct gccgccgatt attttattgc caataaaatt gaccagccga aaattgccgt
      661 catcaattgc gaagcctttg aagtttgtgt gcagcgacgt aaaggatttg aagaagtatt
      721 aaaatcccgc gttcccggcg cgcaaattgt cgctaaccaa gaagggactg ttttagataa
      781 agcgatttcc gttggtgaaa aactgattat ctccacgccg gatctcaacg ccattatggg
      841 ggagtcgggc ggtgcgacac tcggcgcggt aaaagcggta cgtaatcaaa atcaggccgg
      901 aaaaattgct gttttcggtt cggatatgac aaccgaaatt gctcaggagc tggaaaacaa
      961 tcaggtgctg aaagcggtag tggatatttc cggtaagaaa atgggcaatg ctgttttcgc
     1021 gcaaacattg aaggttatca ataaacaagc cgacggtgaa aaagtgattc aggtgccaat
     1081 cgatctctat accaaaacgg aagatggtaa acagtggctg gcaacgcacg ttgatggtct
     1141 gccctaatcg ctgattcgca gaggtcccta tgttcacggc aacagaggca gtcccggtag
     1201 caaaagtggt ggcaggaaat aagcgttacc ccggcgtcgt tgcgctggat aacgttaact
     1261 tcacgctcaa taaaggcgaa gttcgtgcgc tgttaggtaa aaacggcgcg ggcaaatcga
     1321 ctctcattcg aatgcttacc ggcagcgaac gcccggatag cggtgatatc tggattggcg
     1381 agacgcgact ggaaggtgac gaagctacgt tgactcgccg tgccgctgaa ctgggggttc
     1441 gtgcggttta tcaggaatta agtctggtgg aagggctgac ggtggcagaa aatctctgcc
     1501 ttggtcaatg gcctcgccgc aacggcatga ttgattacct gcaaatggcg caggatgccc
     1561 aacgttgctt acaggcgctg ggcgttgacg ttagtcctga acaacttgtt tcaacgctaa
     1621 gcccggcgca aaagcaactg gtggaaattg cacgggtgat gaagggcgag ccgcgcgtgg
     1681 tcattcttga tgaacccacc agctcgcttg ccagtgcaga agttgaactg gtgatcagtg
     1741 cggtgaaaaa gatgtcggca ctgggcgtgg cggtgattta tgtcagccac cggatggaag
     1801 aaatccgccg cattgcctcc tgtgccaccg ttatgcgcga tggtcaggtg gcgggcgatg
     1861 tgatgctcga aaacacctcc acgcatcata ttgtgtcgct aatgctaggg cgtgatcacg
     1921 ttgatattgc gccggttgcc cctcaggaaa ttgtggatca ggccgtactg gaagtccgtg
     1981 cgttacgcca taagcccaag ctggaggata tcagctttac gctacgtcgt ggcgaagtgc
     2041 ttggcattgc tggcctgctg ggggcagggc gcagtgaatt gctgaaggcg attgttgggc
     2101 tggagacgta tgaacagggc gaaattgtta tcaacggcga gaaaatcatg cgccccgatt
     2161 acggcgacat gctgaaacgc ggcattggct atacgccaga aaaccgcaaa gaagcgggga
     2221 tcattccctg gctgggcgtt gacgaaaata cagtgctgac caatcggcaa aaaatcagca
     2281 ccaacggtgt gctgcaatgg tccaccatcc gccgcctgac cgaagaggtg atgcagcgga
     2341 tgacggtcaa ggctgccagt agcgaaacac ccatcggcac gctttctggt ggcaatcagc
     2401 aaaaagtggt gatcggtcgt tgggtctatg ccgccagcca gattttgttg ctcgacgagc
     2461 caacgcgcgg cgtcgatatc gaagccaaac agcagattta ccgtattgtc cgtgagctgg
     2521 ctgccgaagg aaaaagcgtg gtgtttatct ccagtgaagt ggaggaattg ccgctggtgt
     2581 gtgaccgcat cctgttatta cagcacggca cgttctcgca ggagtttcac tcgccggtca
     2641 atgtggatga gctgatgtcc gccattctgt ctgtgcactg atgaatttta ccgaggaacc
     2701 tgaagatgtc tgcttcgtca ttaccattgc cgcagggcaa gagcgtctcg ctcaaacaat
     2761 ttgtcagtcg ccatattaat gagatcggtt tgctggtggt gattgccatt ctttatctgg
     2821 tcttctccct gaacgcgcct ggctttatct cattgaataa ccagatgaac gtgctgcgcg
     2881 atgccgccac cattgggatt gccgcctggg cgatgacgct gattattatc tccggtgaaa
     2941 ttgatgtcag cgttgggccg atggtggctt ttgtctcggt gtgcctggca tttttgctgc
     3001 aatttgaagt tccgttggcg attgcttgtc tgctggtgtt gctgttaggc gcgctgatgg
     3061 gaacgctcgc cggggtgctg cgcggcgtgt ttaacgtacc aagtttcgtt gccacactgg
     3121 ggctgtggag cgctctgcgc ggaatggggc tgtttatgac caacgcacag ccagtgccga
     3181 ttgacgaaaa cgaggtgctg gactggctgg gcggacaatt tctcggtgtg ccagtatccg
     3241 cgctgatcat gatggtgttg tttgcgctgt ttgtgttcat tagccgaaaa accgccttcg
     3301 ggcgctcggt ttttgccgtt ggcggtaatg ccacggcggc gcagttgtgc ggtattaacg
     3361 ttcgtcgagt gcgcattctt atctttaccc tttcgggatt attagcggcg gtgaccggca
     3421 ttttgttggc ggcacgcctc ggttccggta acgcaggtgc cgcaaacggt ctggagtttg
     3481 acgtcatcgc cgcggtcgtg gtcggcggta cggcactttc cggcggtcgc ggttccctgt
     3541 tcggtacatt gcttggtgtg ctggtgatta cgctaatcgg taacggtctg gtgctgctcg
     3601 gtattaactc ctttttccag caggtggtac gcggcgtcat catcgtggtg gcggtgctgg
     3661 cgaatatctt gctgacccag cgaagcagta aagcgaaacg ctaaacctta acgcccctgg
     3721 cgcgggtgcg gcagggatga aaattaatcg tattgttaat gagggattta tgaaaacgat
     3781 gctggcagct tatttaccag gaaattcaac cgtcgatctg cgggaagttg cggtgccgac
     3841 gccggggatt aaccaggtac tgatcaaaat gaaatcctcc gggatttgcg gaagcgatgt
     3901 ccactatatc tatcatcaac accgtgccac ggcggcggca cccgataaac cgttatacca
     3961 gggatttatc aacggtcatg aaccgtgcgg gcagattgtg gcgatggggc aaggctaccg
     4021 ccattttaaa gagggcgacc gcgtgctggt gtatcacatt tctggctgtg gtttttgccc
     4081 gaactgccgc cgcggctttc ctatctcttg tactggcaaa ggaaaagcgg cttacggctg
     4141 gcagcgtgac ggcggtcatg ccgaatatct gctggcggaa gaaaaagatc tgatcctcct
     4201 gccggatgcg ctgagctacg aagatggtgc gtttatcagt tgcggcgttg gcacggccta
     4261 tgaagggatt ttgcgcggcg aagtttccgg cagcgataac gtgctggtgg tcggtctggg
     4321 ggcggtcggc atgatggcga tgatgctggc gaaaggtcgc ggtgcaaaac ggatcatcgg
     4381 cgttgatatg ctgccggaac gtctggcgat ggcaaaacag ttagggggga tggatcacgg
     4441 ctatttagca accaccgaag gtctgccgca gataattgct gaacttaccc acggtggcgc
     4501 ggatgttgcg ctcgattgtt ccggtaatgc cgcaggtcgc ttgctggcac tgcaatccac
     4561 tgctgactgg gggcgggtgg tttacattgg tgaaaccgga aaagtggaat tcgaggtcag
     4621 cgctgatctg atgcaccatc aacggcggat tatcggctcc tgggtgacca gtctgttcca
     4681 tatggaaaaa tgcgcccacg atttgactga ctggaagctg tggccgcgta acgccattac
     4741 ccatcgcttc tcgctagaac aggcaggtga tgcctatgtg ctgatggcga gcggcaaatg
     4801 cgggaaagtt gtgattaact tcccggatta aggagttagc ggatgaccat ctatacctta
     4861 tcccacgggt cgctaaagct ggacgtttct gaccagggcg gcgtaatcga aggcttctgg
     4921 cgcgatacga cgccgctctt acgtcctggt aaaaaaagcg gtgtggcaac cgatgcctca
     4981 tgttttccgc tggtgccatt tgcaaaccgg gtgagcggca accgttttgt ctggcagggg
     5041 cgtgagtatc aactgcaacc gaatgttgag tgggatgcgc actatctaca cggcgatggc
     5101 tggctgggcg agtggcaatg tgtctcgcac agcgatgata gtttgtgtct ggtgtatgaa
     5161 catcgcagcg gtgtctatca ctatcgggta agtcaggcgt ttcatttaac ggcggatacg
     5221 ctgacggtga cgctctctgt caccaatcaa ggggcagaga cgctgccatt tggtaccggc
     5281 tggcatcctt attttccgtt gtcaccacaa acgcggattc aggcgcaggc gagcggttac
     5341 tggctggaac gggagcagtg gctggcgggt gagttttgcg agcagctacc gcaggaactg
     5401 gattttaacc agctcgcgcc gttgccgcgt cagtgggtga acaatggttt tgccggatgg
     5461 aatggtcagg cccgcatcga gcagccgcag gaaggttatg ccatcatcat ggaaacgacg
     5521 ccacctgcac cgtgttattt catctttgtt tccgaccctg cgtttgataa aggatatgcg
     5581 tttgatttct tctgtctgga accgatgagc catgcgccgg atgatcatca tcgcccggaa
     5641 gggggtgatc tcattgcgct agcaccaggg gaatcaacaa cctcagagat gtcgttgcgg
     5701 gtagagtggc tgtaatttat aaaatgccgg ataaggcgtt cacgccgcat ccggcatctt
     5761 tcctaacgcc gatcgagaga aattgccccc ggtccggtaa ttgccaatag caagaacgcg
     5821 ccagcgatac tgacattctt ccagaaatta atcatatttg gcccaaccgc atcgccggtc
     5881 atatcccagt aatggtgacc aatcaccgcc gtacccagcg tgtagaaaat aaacagcacc
     5941 gccagcggac gggtgaaaaa gccaagcacg attaatatcg cggcgggcac ttccataact
     6001 accgcaataa tcgctgccag catcggcatt ggcgcgccca acgaggccat atattggacc
     6061 gtaccgtcaa agcccatcat tttgggaaaa ccaaaaataa tgaaaattaa gaccacggcg
     6121 atacgggcaa ttaataacaa aacggggcgg gcagctccaa aatcaaaata acgtagtgtg
     6181 ttcataacca atccctttgc aagataatgt gatttaaacg taatacacac tcttgcacct
     6241 cgccacgccc caggcattga gatattcgct aattatcctg gttacagtga tttaagcgcg
     6301 atgttctcat ctatcagcag tttcgcatca aacgttttgc cgctctggat ccatttgcga
     6361 attgggcgaa tctcacgccc ctgattcagc gtgaccgcac cgataagcac gccgttttgc
     6421 agattaaacc aaatcgcctt ctgagtttct gggttgccac gacaaagcta gtcatcgcca
     6481 cgcatatcgc caataaactg taagttatca ctgtactgat cgctccagaa ccacggcggc
     6541 ggcagtagcg gtagcggtag ccctaacatt gcggcagcgg caatttgcgc ctggttattg
     6601 gcgttttccc agctttcgca gcggtgtagt gcaccattat caagacgagt gattgccaca
     6661 tcgccaccgg caaagatcgc gggatcgcag gtgcggcaag cctcatcaat gacaatgcca
     6721 ttggcagtat caaggttggc ctcgcgagcc agttgctcgt tggcgctgat actaataccg
     6781 taaatcacca catcagcctg aagcgtttcc ccactttgca gcgtcagttc tactttttca
     6841 ccatcgacca catgttcaat ggcattattg agcagaatgc gcacaccagc ctgctgatgg
     6901 cgctgtaaaa gatagcgttg cacgggcggt ggtgcattac ggcccatgac ggttgccgcc
     6961 agttcaatca ctgtcacctt acatctgcgc tgcgtggcgc tggcagccag ttccagacca
     7021 atagttccgg caccgacaat cacgactgac cgttcgggct gcagaacttc tcgcagtctg
     7081 gcggcatcac cggcatggtg tagggtaaag cagcgttctc ccagtgcatc aagcaacggc
     7141 agcggtcgag ctgccgcgcc ggttgctata aaaagctgat cccagtgcca gctttcgccg
     7201 ttggttaaca ctaactctcg tgtgtcgcga cccagcgttt tgatggttac accggaatgc
     7261 agatgaacat tgttttcctg ccaccagtta gcgggtaaca cctgctgtaa ctgcggggaa
     7321 tcttccagca acatggattt cgagagtgga ggtcgttcat aaggaagatg tcgctcatcg
     7381 gaaaacagat gcagctcacc ggtgaaccct tgctggcgta gcgaggccgc agccattgcc
     7441 gccgcttgcc cgccaccgac aataatgatc gttttttctt tcatcgtgct tccttaaaga
     7501 tccagcccag ccgctacgtg gcgaatgccg cgaatcgcta aacccgcatc agcgttgatc
     7561 atcacaccgc ttaatgcgcg attattgcgc cgcgatgcca acatcacata cggccccgta
     7621 aaatccgccg gttgcgggaa aaattgcagc ggtaaaatgg cggcaatttt ctccggcgtc
     7681 agagactgca ttatcgaggt ttcactttgc ccgagcgcct gtgggccgcg caggtcgctg
     7741 gccataccac acgggccgac gccattcacc cgcactttcg gtgccagttc ataagccagt
     7801 tggcgaataa gtccggttgc ggcatgttta ctggtggtgt acagcgggcc accgccgcca
     7861 ggataccagg cggcatttga cagtgtgaaa atcatgctgc cttcactggc gattaacgcc
     7921 ggagcgcagg cttttgcgcc cagcaggtaa ccgagtacgt tgacgttaaa cagctcgtgg
     7981 aagccggttt cgagcgtctc tgcgggagta ttaaccagtg aggcattgtg atcccagatg
     8041 cctgcattgc cgataaaaca atccagcttg ccggaacgag tcaggatctg atcgaccgcg
     8101 cgttgataat cggcataaca ggtcacgtta ccttccaccg ccagaatatg ttcgccaaat
     8161 cgctgacgca gactggcgac ttttgccgcc gacagttcca gcgcggcaac ctgcgcgcct
     8221 tcttcgataa atcgctcgac cagcgccagc cctaatcccg atccgccgcc ggtaataaaa
     8281 atggactcgt tatgcagatc gctcatggct gcgcctccgg taagtcgatg aaaatgtcac
     8341 caccttcaac gtgtactgga taagtggtga gcggatcggt ggcgggcagg cataacgctt
     8401 tccccgtttt caggcaaaaa ctggcggcgt gtagcgggca ctccaccgtg gcgtcatctt
     8461 ccagataccc ttctgacatc gacgcattac catggctgca acgatcgtta atggcataaa
     8521 actcgccgcc cacgttgaac aaggcgatga cgggcgaggt atctatccgg agagcctcac
     8581 cctccggcac atccgcaacg ggacacgcat aaattcgatt catcagaaca gtacactcag
     8641 gttatgggaa gtgatcaccg cttgatccag gacgatatcc cgttccagca agcgccagtt
     8701 gtcatcttcc agacggcgaa ctttgtcgaa acgcgttcca acatagaatg tttcatcgcg
     8761 ctctttttgt gcccgataaa gtagataatt caccctcacg gcaaatacgt ttgggatgtc
     8821 ggtttcgctt acctggcagt tgctgattaa gtgacgagtg cgtgacggcg gctcttctgc
     8881 ccaggccatg cccgtttcca gacgggcgat tcgccgctcc agctggtctt tggtgtcatt
     8941 gaaaatccag gtcgtcggcg gctggacgcc tttgcgacgg tcgcgtgttt gcgcgttaac
     9001 cgtggtgcgc atggtgtaac gaatctcttc gtcgagctgt gccaaccagt cacgaaattt
     9061 ccagtcatcc agcagactgg cttcgtgaaa gagaaactgg ctaatgcggt gatgtaactc
     9121 cagtgaaact tgcgcactca tttcatcacc tcctgctggt aagcggcggt tttatcgagc
     9181 acttcctgcc agctttcgct actcagaaga tcggcccagc gttggtacat tccacgagcg
     9241 gcagtttctg aaaagatata gttagtaatg ccaggaatgc cgtcgtcgcg gcgcttttcc
     9301 tgaccaagcc ccatttccag acacagtttg ctgttgcggg cgcggtggcc tttaagcaat
     9361 ttctggattt cacaccagtt ctccgagtca tcctgctcga gaaaaccagc aggaccaaaa
     9421 gcacgagtgg cgctgttttc aaaagcggct ttaacttcat cggaggcggc tttgtcagta
     9481 atacagaacg cccacacttc aacttgatca gggccgcgcg gatgccagac gcggagtgtg
     9541 gcagtgccgt ttagccatga aagcgtgggg aatatattgt tatgacccgc caggcgcagg
     9601 gcgcgaactt cacctaaacg ttgttctgct tcggcatagg tttcgcggta atagcttgaa
     9661 actgcgccat cgacccagac attagcatcc ggtttttcag taaagaagaa accgctaccg
     9721 tgaccgtcct gaccaaattg cagcgcatct ttggcggttt cccacaccgg gcgggcggtt
     9781 tgtccatcac cgaggcgctt atcgctgcca tcatctttcg cccctaatac ctgaacggca
     9841 gaagcatggc tgaacagagc atgatactgg tcactggcga actgctctgc cgggaatttc
     9901 cagttacagt tgatcaccca cttttgtacg ccgccgacaa tttcggtgcc gccttcgcga
     9961 cgatccagca tgccatccag ataccaggca atgtcaccca ggtaatcatg caggcccggt
    10021 gcgctggtat cccagttgcc aaaaatcagt cctttataac tctccacaca aggaacttcg
    10081 tttagccccc agtgggattt acacaaccct tgtgggtagg cgcgaggttc cagcggtaca
    10141 tcgatcaact cgccgttaat gccataagac cagccgtgat acgggcaggt aaaggcgcga
    10201 gtgttgccac aatctgcata actcacacgc atggcccggt ggcggcattg gttaagaaac
    10261 gccttgatgc tgccgtcttt ctggcgcacc acgacaactg catcttctcc catgtaggtg
    10321 ttaaagaaat caccgggttt tgggatctgg ctttcgtggg cgagaaatag ccagcaacga
    10381 ccgaaaatac gctcaagctc cagttggtaa atgtccgggt cggtataaat acgcggagtg
    10441 acccgaccgt tttgggtgtc aatcagttgg taaatgttca aatctgaggg tgtggtcata
    10501 ttttttaatc ctgaaagata agatgtattg accttctatt tttgtgccat aaggatgttg
    10561 ctggttggtt gctaatatgt gaaatagtta atttctacca gctgagacgt tttttcgata
    10621 acaaggggaa ggtgatggaa ctacggcatt tacgctattt cgtcgcagtg gcgcaggcac
    10681 tgaactttac ccgtgcggcg gaaaaactgc atacctcaca gccttcgtta agcagccaga
    10741 tccgcgatct tgaaaactgt gtcggtgttc cgttactggt gagggataag cgcaaagtcg
    10801 cgttgacggc ggcgggagaa tgttttctcc aggacgcgct ggcaatcctc gaacaagcgg
    10861 aaaatgccaa attacgggcg cggaaaattg ttcaggaaga cagacaatta accattggct
    10921 ttgtgccatc ggcggaagtg aatttattgc caaaagtatt accgatgttt cgtctcagac
    10981 agccagacac cttgattgag ctggtgagtt taatcaccac gcaacaggag gaaaaaattc
    11041 gccgtggtga actcgacgtc ggcttaatgc gccatcctgt ttatagcccg gagattgatt
    11101 atctggagct ttttgacgaa ccattagtgg tggtgttacc ggttgatcac cctttagcac
    11161 atgaaaaaga gatcaccgcc gcgcaactgg atggcgtgaa tttcgtcagt accgatccgg
    11221 cgtattccgg ttcgcttgcg ccgatcgtta aagcgtggtt tgcgcaagaa aacagccagc
    11281 caaatatcgt ccaggtggca acgaatattc tggtgaccat gaatctggtg ggcatggggc
    11341 tgggcgtcac tttgataccc ggttatatga ataattttaa taccgggcag gttgtttttc
    11401 gtcccattgc cggaaatgtg ccttccattg ctttactgat ggcgtggaag aaaggggaga
    11461 tgaagccggc attacgcgat ttcatcgcca ttgtgcagga acgtttggca agcgtaacgg
    11521 cataattacg cattattttt gcaacatgcg ccgcggatcg cagtttttta gtgcttgtga
    11581 actggcgtta cagacatcgg gaagcaggct cgaatattct tatttttcaa cttgcaccaa
    11641 cattcgatta accccagact aacgcctcct gacgggaggg actcatggtt ttgcaatcca
    11701 cgcgctggtt ggcgctcggc tatttcacat acttttttag ttacggcatt tttttacctt
    11761 tctggagcgt ctggcttaaa gggattggtt taacgccaga aaccatcggc ctgttattgg
    11821 gggcaggtct ggttgcccgt tttctcggga gtttgctcat cgcgccccgc gtcagcgatc
    11881 cttcccgcct gatttccgcc ttgcgcgtgc tggcactgct gccacttctc tttgctgtcg
    11941 ccttctgggc gggggcgcac gtagcgtggc tgatgctggt gatgattggc tttaacctct
    12001 ttttctcacc gctggtaccg ttgaccgatg cactggcgaa tacgtggcaa aagcagttcc
    12061 cgcttgatta cggcaaagtg cgactgtggg gctcggtggc gtttgtcatt ggctcggcgc
    12121 tgacgggcaa actggtcact atgtttgatt atcgggtgat cctcgtgctg ttgacgttgg
    12181 gcgtggcatc catgctgctc ggctttctca tccgtccgac gattcagcca caaggggcaa
    12241 gccgccagca ggagagcacc ggttggtctg cgtggttggc gctggttcgc cagaactggc
    12301 gctttctggc ctgcgtttgt ttattgcagg gggcacatgc ggcctattac ggttttagcg
    12361 ccatttactg gcaggcagct ggctactcgg cctcggcggt ggggtatttg tggtcgctgg
    12421 gcgtggtggc ggaagtcatt atctttgcgc tgagtaataa acttttccgc cgttgtagtg
    12481 ctcgcgatat gctgttgatc tcggcgattt gcggcgtagt gcgctggggc attatgggag
    12541 caactacggc gttgccgtgg ttgatagtgg tgcaaattct gcattgcggc accttcacgg
    12601 tctgccacct ggccgccatg cgttatattg ctgctcgcca ggttagcgaa gtcatccgtt
    12661 tacaggcggt ttactctgcc gtcgcgatgg gcggcagtat cgctatcatg accgttttcg
    12721 ccggtttcct gtatcaatat ctgggccacg gtgtgttctg ggtaatggcg ctggtggcgc
    12781 ttccggcaat gtttttgcgc ccgaaagttg ttccctcatg ctgattcgag cattttgcgg
    12841 atctgctcct gttgaggtgt tgtcagcgtc aggtccgtat agatcagcgg tggtgaaaag
    12901 agcggtaacg gcatggtata aggcgcaata atcagtgccg cgccacgcgg agcgcctgtc
    12961 tgcaaaaatg cctttaccga catatgctta atgttgagcg gcagtaacgt tagttcgcgt
    13021 aactgctgct caatctgcgc ttctcgctcg ctatcattcc cggtcagtag aataatctgt
    13081 ttttcatgca gatcgttttc ctgcattagc caggcaccga aaatcaccgc gaccagacca
    13141 ctttcctcat cagaaagatg gacgccgtat tcactttcaa atccggccag cgccgcgcgg
    13201 gtgatgcgta ccaggcgtgg atacagtctg gcgaactctt ccggcagggt attatcaata
    13261 ccgatggcaa acaaactgcg atttaacgcc tgggcgaggt ggatataaag ctgatcacat
    13321 aacccctgtt catcataaaa acgaacattt cccagctcac gaaaatggtt taccagacgt
    13381 ttgatagact gacgcaattg tctgtcccgc tgatgcgcat cccgcagtgg atcgggaacc
    13441 cgcaacatgg aaaaaagtaa tgccataaac agtggctcat caggcggtac atgctggagc
    13501 gcccgacgct gccagtggcg tccaatttcc tgcgctacct gaaattcaag gcaggattcc
    13561 gcccagcgac gttggagcgg attaaactga ggcgtaattc cagcgtggtg ctgcagcaaa
    13621 caatattgca gatacagaca caggaaatga atatcgcgcg attcaaagcg tttttgcagc
    13681 cgtcgggagc agagattcac taacgcttgt agattggtat cgtcgtaaaa gttacgcgca
    13741 attccgcgcc gcttcagttc actcttcagg gcgggggtga attggctggt aataaatgac
    13801 ggacacagac ggaaaccacg tcgtagccag tgaaataaac acaaacgttg gttaagcact
    13861 gtaccttcaa cccggtagct accgtcataa ccagtcgtca gtgtgagttg atgatagcgc
    13921 aggatctccc gtcctgtttc gctgatatca agactggcaa tatcatcatc cacaccatta
    13981 agctcgctga aggttgccat ggtggcggtc aaccccggct gaaagagcgt cagcaagatc
    14041 tggcagcggc gctggggagc cgaaaggaca gatggtggag caagcgtagg catcatctgt
    14101 agatctccca taagcataat tgataagaat agctaaagtt tgctcaggga aatggcgaag
    14161 ggaatgctaa tcatcagaaa tgttggcaag cgtcacagaa tttttctcat tttaaactgt
    14221 tctgaccagt ggattagcat gtcttaaggt ggttttgtga atgattatcg tgctagcgat
    14281 catgggttag ttctgcggat tcaacgcgct aaggataaag tccaccatct gttcgcgata
    14341 aacattgccg tgacgatcgg aaaaagcatc aatgtgttcg ccatccggga ttaggatcaa
    14401 ccgttttggc tcttttgcca ggctatacaa cttttcgctg tgctgccatg ggataacgtg
    14461 atcggcttta ccgtgaatga gtaaaagcgg gatcgggctg acgctggcga tgtaattttc
    14521 gccgctgtaa ctctcatcaa gtaagtagcc actgccgggg agcatctggt tggcgatggt
    14581 tgcataagag gcaaatgtgg agtcgaggat caccgcacgt atgccttcac gatcaccctg
    14641 accaataaca gcaagaatat tcgccccgcc aatactctgc ccgaacagca ccagccgttg
    14701 tgggtttaca tcactgcgat ggcgcaccac attgatggcg ctttgcgtat cgtccagcaa
    14761 tccggcctgg gacggagtgc cttttgattt accaaacccg cgataatcaa acataaaaac
    14821 gttgaaatta cgctcaggta accaactgac cagcggccag tgggcggaca tatttccggc
    14881 attgccgtga gcatgaatga tggttgcgat ggcgttgtca gcagggcccg tcgaagaagg
    14941 aataaaccag ccttgcagac gagtaccgtc cttagccgta aattcgacgg attccgccga
    15001 ccaggggtcg ggaccgtaaa ttttatcgtc aggataatag aagaagttga tggcaacgcg
    15061 cagcactaaa taaacgcaga aggcaacaac aaagagaata aacagaattt tgggaacgcg
    15121 tttgttcact ggcagtgcca taacctttcc ttgcgacttc tttatcatcc attaatcggg
    15181 gaaagatggt gggagagtga cgaaagaaag taaagcggtg aggaagtatt tcggaatagc
    15241 gcctgaaagg cgagggcggg tgagtgatat cacccgcctg agtcattaac gcttcagagc
    15301 gtcgcttaac tcgtcacgca tgttcgccag tatggctttc acaacacgcg ggttaccagc
    15361 aacgatgtta ccggtcagca tatagttatg accaccggtg aagtcgctga cgatgccgcc
    15421 cgcttcacga accagcagat cgcctgccgc gaaatcccac ggacgcagac cgatttcaaa
    15481 gaaaccgtca acacgacccg cagcgacgta agccagatcc agcgccgcag aaccggtacg
    15541 acggaagtct gcacattcgt tgaacagctt gccgacgatg ttgatgtagg tagtggcgta
    15601 ctgttttgct ttgaacggga agccggtcgc cagaatggta ccgtcgagat cgcgagcggt
    15661 gctaccgcgc agacggtagc cgttcagctg tgcgccctga ccgcgagtgg cggtgaacag
    15721 ttcgttacgc ataggatcgt ataccacagc aacttcggtg cggcctttga tacgaacggc
    15781 gatagatacc gcgaagtgcg gcagacgttt gataaagttg gtagtaccat ccagtggatc
    15841 gataacccat tgaacatcct gatcagtacc ttcaagttca ccgctttctt cggtgatgat
    15901 ggtgtgctgt gggtaagatt tacgaatcgt gtcgataatc accgcttcgg cagctttatc
    15961 tacgttggtc acgaaatcgt tactgccttt ctggctcgct tctacagcgt ccggggtttc
    16021 atagtttttg gcaattaaat tacccgcctt gcgcgctgcg cgcacggcga tgttcagcat
    16081 cggatgcatc agtctctctc actggatgtt aaagaacggg aaaacggcgc agagtatagc
    16141 agcgagatcg gcatctgtct tcgttttatg ataagatgcg tgaataatct ttagatgaag
    16201 aaagacaatg ctgcaaaata ttcgaattgt gctggtggag acgtcacaca ccggcaatat
    16261 gggttctgtt gcccgtgcca tgaaaacaat gggattaacc aatctgtggc tggttaatcc
    16321 actggtgaaa cccgactccc aggcgattgc cctggctgca ggggccagcg atgtgattgg
    16381 taatgctcac atcgtcgata cgctcgacga agcgttagcc ggttgtagcc tggtggtggg
    16441 caccagcgca cgctcccgca cgctgccgtg gccgatgctc gacccgcgcg aatgcggtct
    16501 gaaaagcgtc gctgaagcgg caaatacccc ggtggcgctg gtgtttggtc gtgagcgcgt
    16561 cggcttgacc aatgaagagt tgcagaaatg ccattatcat gtcgcgattg cggcaaaccc
    16621 ggaatacagt tcgctgaacc tggcaatggc ggttcaggtt attgcctatg aagtgcgtat
    16681 ggcctggctg gcaactcagg aaaatggcga gcaggtcgag catgaagaga cgccgtatcc
    16741 gctggtcgat gatctggagc gtttttatgg tcatctggaa caaacgctgc tggcaaccgg
    16801 ttttatccgt gaaaaccatc cggggcaggt gatgaataaa ttgcgccgtc tgtttacccg
    16861 tgcgcgcccg gaaagccagg aattgaatat cctgcgcggg attctggctt ctattgagca
    16921 gcagaataaa ggtaacaagg ccgaataaca gtggttgcct gatgcgacgc ttaacgcgtc
    16981 ttatcaggcc tacaatgtac agaatcgtag gtcggataag gtgttcacac cgcatccgac
    17041 aacaggtaca aacgccacga taaaaaaatg gcactgaagg ttaaataccc gactaaatca
    17101 gtcaagtaaa tagttgacca atttactcgg gaatgtcaga cttgaccctg ctatgcaata
    17161 cccccacttt tacaataaaa aaccccgggc aggggcgagt ttgaggtgaa gtaagacatg
    17221 agactgacat ctaaagggcg ctatgccgtg accgcaatgc ttgacgttgc gctcaactct
    17281 gaagcgggcc cggtaccgtt ggctgatatt tccgaacgtc agggaatttc cctttcttat
    17341 ctggaacaac tgttttcccg tctgcgtaaa aatggtctgg tttccagcgt acgtggacca
    17401 ggcggtggtt atctgttagg caaagatgcc agcagcatcg ccgttggcga agtgattagc
    17461 gccgttgacg aatctgtaga tgccacccgt tgtcagggta aaggcggctg ccagggcggc
    17521 gataaatgcc tgacccacgc gctgtggcgt gatttgagcg accgtctcac cggttttctc
    17581 aacaacatta ctttaggcga actggttaat aaccaggaag tgctggatgt gtctggtcgt
    17641 cagcatactc acgacgcgcc acgcacccgc acacaagacg cgatcgacgt taagttacgc
    17701 gcttaataaa aagaattcag aatcaggccg gagtgctaaa tactccgtaa acacggtcgt
    17761 acatccagcc ggtagcctga ttccttgcat tgagtgatgt acggagttta tagagcaatg
    17821 aaattaccga tttatctcga ctactccgca accacgccgg tggacccgcg tgttgccgag
    17881 aaaatgatgc agttcatgac gatggacgga acctttggta acccggcctc tcgttctcac
    17941 cgtttcggct ggcaggctga agaagcggta gatatcgccc gtaatcagat tgccgatctg
    18001 gtcggcgctg atccgcgtga aatcgtcttt acctctggtg caaccgaatc tgacaacctg
    18061 gcgatcaaag gtgcagccaa cttttatcag aaaaaaggca agcacatcat caccagcaaa
    18121 accgaacaca aagcggtact ggatacttgc cgtcagctgg agcgcgaagg tttcgaagtc
    18181 acctacctgg caccgcagcg taacggtatt atcgacctga aagaactcga agcggcgatg
    18241 cgtgacgaca ccatccttgt ttccatcatg cacgtaaata acgaaatcgg cgtggtgcag
    18301 gatatcgcgg ctatcggcga aatgtgccgt gctcgtggca ttatctatca cgttgatgca
    18361 acccagagcg tgggcaaact gccaatcgac ctgagccagt tgaaagttga cctgatgtct
    18421 ttctccggtc acaaaatcta tggcccgaaa ggtatcggtg cgctgtatgt gcgtcgtaaa
    18481 ccgcgtgtac gcatcgaagc gcaaatgcac ggcggcggtc acgagcgcgg tatgcgttcc
    18541 ggcactctgc ctgttcacca gatcgtcgga atgggcgaag cctatcgcat cgcaaaagaa
    18601 gagatggcga ccgagatgga acgtctgcgc ggcctgcgta accgtctgtg gaacggcatc
    18661 aaagatatcg aagaagttta cctgaacggt gacctggaac acggtgcgcc gaacattctc
    18721 aacgtcagct tcaactacgt tgaaggtgag tcgctgatta tggcgctgaa agacctcgca
    18781 gtttcttcag gttccgcctg tacgtcagca agcctcgaac cgtcctacgt gctgcgcgcg
    18841 ctggggctga acgacgagct ggcacatagc tctatccgtt tctctttagg tcgttttact
    18901 actgaagaag agatcgacta caccatcgag ttagttcgta aatccatcgg tcgtctgcgt
    18961 gacctttctc cgctgtggga aatgtacaag cagggcgtgg atctgaacag catcgaatgg
    19021 gctcatcatt aatcggtatc ggaatcagga gaatttataa tggcttacag cgaaaaagtt
    19081 atcgaccatt acgagaatcc gcgtaacgtg ggttcctttg ataacaacga cgagaacgtc
    19141 ggcagcggca tggtgggtgc accggcctgt ggcgacgtaa tgaagttgca gattaaagtc
    19201 aacgatgaag gtatcattga agacgcgcgt tttaaaactt acggctgcgg ttccgctatc
    19261 gcttccagct ccctggtcac cgaatgggtg aaagggaagt ctctcgacga agcgcaggcg
    19321 atcaaaaaca ccgatattgc tgaagaactt gaactgccgc cggtgaaaat tcactgttct
    19381 attctggcag aagacgcgat caaagccgcc attgcggact ataaaagcaa acgtgaagca
    19441 aaataagagt tgaggtttgg ttatgtcgat tacactgagc gacagtgcag cagcgcgagt
    19501 aaataccttt ctggctaacc gcggcaaagg gtttggcctg cgtctgggcg tgagaacctc
    19561 cggatgttca ggtatggctt atgtactgga atttgttgac gaaccgacgc cggaagacat
    19621 cgtgtttgaa gacaaaggcg tgaaagtcgt ggtcgatggc aaaagcctgc aatttctgga
    19681 cggtacgcag ctggacttcg taaaagaagg cctgaatgaa gggtttaaat tcaccaaccc
    19741 gaacgttaaa gatgagtgtg gttgcggcga aagcttccac gtttgatgcg cataccgata
    19801 accccaccgt ggtcgcctgc gcgtggggtt tgttttacct gattcgccgt tatcgcggcg
    19861 gatcgcagcc ctgagaatgt tatggattac ttcaccctct ttggcttgcc tgcccgttat
    19921 caactcgata cccaggcact gagcctgcgt tttcaggatc tacaacgtca gtatcatcct
    19981 gataaattcg ccagcggaag cctggcggaa caactcgccg ccgtacagca atctgcaacc
    20041 attaaccagg cctggcaaac gctgcgtcat ccgttaatgc gcgcggaata tttgctttct
    20101 ttacacggtt ttgatctcgc cagcgagcag catactgtgc gcgacaccgc gttcctgatg
    20161 gaacagttgg agctgcgcga agagctggac gagatcgaac aggcgaaaga tgaagcgcgg
    20221 ctggaaagct ttatcaaacg tgtgaaaaag atgtttgata cccgccatca gttgatggtt
    20281 gaacagttag acaacgaggc gtgggacgcg gcggcggata cggtgcgtaa gctgcgtttt
    20341 ctcgataaac tgcggagcag tgccgaacaa ctcgaagaaa aactgctcga tttttaattt
    20401 ctggaagcta aacatggcct tattacaaat tagtgaacct ggtttgagtg ctgcgccgca
    20461 tcagcgtcgt ctggcggctg gtattgacct gggcacaacc aactcgctgg tggcgacagt
    20521 gcgcagcggt caggccgaaa cgttagccga ccatgaaggc cgtcacctgc tgccatctgt
    20581 tgttcactat caacagcaag ggcattcggt gggttatgac gcgcgtacta acgcagcgct
    20641 tgacaccgcc aacaccatta gttctgttaa acgcctgatg gggcgctcgc tggctgatat
    20701 ccagcaacgc tatccgcatc tgccttatca attccaggcc agcgaaaacg gcctgccgat
    20761 gattgaaacg gcggcggggc tactgaaccc ggtgcgcgtt tctgcggaca tcctcaaagc
    20821 actggcggcg cgggcaactg aagccctggc aggcgagctg gatggtgtag ttatcaccgt
    20881 tccggcgtac tttgacgatg cccagcgtca gggcaccaaa gacgcggcgc gtctggcggg
    20941 gttgcatgtt ctgcgcttac ttaacgaacc gaccgctgcg gctatcgcct acgggctgga
    21001 ttccggtcag gaaggcgtga tcgctgttta tgacctcggt ggcgggacgt ttgatatttc
    21061 cattctgcgc ttaagtcgcg gcgtgtttga agtgctggca accggcggtg attccgcgct
    21121 cggcggcgat gatttcgacc atctgctggc ggattacatt cgcgagcagg cggacattcc
    21181 tgatcgtagc gataaccgcg ttcagcgtga actgctggat gccgccattg cagccaaaat
    21241 cgcgctgagc gatgcggact ccgtgaccgt taacgttgcg ggctggcagg gcgaaatcag
    21301 ccgtgaacaa ttcaatgaac tgatcgcgcc actggtaaaa cgaaccttac tggcttgtcg
    21361 tcgcgcgctg aaagacgcgg gtgtagaagc tgacgaagtg ctggaagtgg tgatggtggg
    21421 cggttctact cgcgtgccgc tggtgcgtga acgggtaggc gaatttttcg gtcgtccacc
    21481 gctgacttcc atcgacccgg ataaagtcgt cgctattggc gcggcgattc aggcggatat
    21541 tctggtgggt aacaagccag acagcgaaat gctgctgctt gatgtgatcc cactgtcgct
    21601 gggcctcgaa acgatgggcg gcctggtgga gaaagtgatt ccgcgtaata ccactattcc
    21661 ggtggcccgc gctcaggatt tcaccacctt taaagatggt cagacggcga tgtctatcca
    21721 tgtaatgcag ggtgagcgcg aactggtgca ggactgccgc tcactggcgc gttttgcgct
    21781 gcgtggtatt ccggcgctac cggctggcgg tgcgcatatt cgcgtgacgt tccaggtcga
    21841 tgccgacggt cttttgagcg tgacggcgat ggagaaatcc accggcgttg aggcgtctat
    21901 tcaggtcaaa ccgtcttacg gtctgaccga tagcgaaatc gcttcgatga tcaaagactc
    21961 aatgagctat gccgagcagg acgtaaaagc ccgtatgctg gcagaacaaa aagtagaagc
    22021 ggcgcgtgtg ctggaaagtc tgcacggcgc gctggctgct gatgccgcgc tgttaagcgc
    22081 cgcagagcgt caggtcattg acaatgctgc cgctcacctg agtgaagtgg cgcagggcga
    22141 tgatgttgac gccatcgaac aagcgattaa aaacgtagac aaacaaaccc aggatttcgc
    22201 cgctcgccgc atggaccagt cggttcgtcg tgcgctgaaa ggccattccg tggacgaggt
    22261 ttaatatgcc aaagattgtt attttgcctc atcaggacct ctgccctgat ggcgctgttc
    22321 tggaagctaa tagtggtgaa accattctcg acgcagcgct gcgtaacggt atcgagattg
    22381 aacacgcctg tgaaaaatcc tgtgcttgca ccacctgcca ctgcatcgtt cgtgaaggtt
    22441 ttgactcact gccggaaagc tcagagcagg aagacgacat gctggacaaa gcctggggac
    22501 tggagccgga aagccgttta agctgccagg cgcgcgtcac cgacgaagat ttagtggtcg
    22561 aaatcccgcg ttacactatc aaccatgcgc gtgagcatta acagaggtta gtatgggact
    22621 taagtggacc gatagccgcg aaattggcga agcactgtac gatgcgtatc ccgatcttga
    22681 tccgaaaacg gttcgattca ccgatatgca tcagtggatt tgcgatctgg aagatttcga
    22741 cgacgacccg caggcatcca acgagaaaat cctcgaagcg attttgttag tctggctgga
    22801 cgaagccgag tgaatgaacg ggctgcctgg gggtagcccg ttttgctgtt aaacgaataa
    22861 ggataactaa aatgacagaa gcgatgaaga ttaccctctc tacccaacct gccgacgcgc
    22921 gctggggaga aaaagcaact tacagcatta ataatgacgg cattaccctg catttgaacg
    22981 gggcagacga tctggggctg atccagcgtg cggcccgcaa gattgacggt ctgggcatca
    23041 agcatgttca gttaagcggt gaaggctggg atgcggatcg ctgctgggca ttctggcaag
    23101 gttataaagg cccgaaaggc acgcgtaaag tggagtggcc ggatctggac gatgcccagc
    23161 gccaggaact ggataaccgc ctgatgatca tcgactgggt gcgtgacacc atcaacgcac
    23221 cggcagaaga attgggacca tcgcaactgg cacagcgtgc tgttgatctg atcagcaacg
    23281 tcgcgggcga tcgtgtgact tatcggatca ccaaaggcga agatctgcgt gagcaaggtt
    23341 atatggggct gcacactgtc gggcgcggtt cagaacgttc tccggtattg ctggcgctgg
    23401 attacaaccc aactggcgat aaagaagcac cggtgtacgc gtgcctggta ggtaaaggta
    23461 tcacttttga ctccggcggc tacagcatca aacagactgc gtttatggac tcgatgaagt
    23521 cggacatggg cggcgcggca acggttaccg gggcgctggc atttgccatt acgcgcggac
    23581 tgaacaagcg cgtgaagctg ttcctctgct gtgcggataa cctgattagc ggcaatgcgt
    23641 tcaagctggg cgatatcatc acttatcgca acggtaaaaa agttgaagtg atgaacactg
    23701 atgccgaagg gcgtctggtg cttgccgatg gtctgattga tgccagtgcg cagaaaccgg
    23761 aaatgatcat tgatgcggcg accctcaccg gggcggcgaa aactgcgctg ggtaatgatt
    23821 atcacgcgct gttcagtttt gacgatgcgc ttgccggtcg tttactggcg agtgcctcac
    23881 aagagaacga accattctgg cgtctgccgc tggcggaatt ccaccgcagc cagctgccgt
    23941 ctaactttgc cgaactgaac aataccggaa gcgcggcgta tccggcaggc gcgagcacgg
    24001 cggcgggctt cctgtcgcac tttgttgaga actatcagca aggctggctg catatcgact
    24061 gctcggcgac ttaccgtaaa gcgccggttg aacagtggtc tgcgggcgct acgggacttg
    24121 gtgtgcgcac gatagctaat ctgttaacgg cgtaaactaa tatgccggat gcggcgtaaa
    24181 cgccttatcc ggcctacaaa ttctgcaaat tcaacaaatt gcgaatccct tgtaggcctg
    24241 ataagcgtag cgcatcaggc atttgcgccc cgaacgttga atgaattgtg gttcttatgt
    24301 ccgaaacaaa aaacgaactt gaagacctgc tggaaaaagc agcaaccgaa ccggcgcacc
    24361 gcccggcctt tttccgtact ctactggaat ccaccgtctg ggtgcctggt acggcggcgc
    24421 agggcgaggc tgtggttgaa gattgtgcgc ttgatttaca gcactgggaa aaagaagacg
    24481 gcaccagcgt cattcctttt ttcacctcgt tagaagcgct tcaacaggcg gttgaagacg
    24541 aacaggcatt tgtcgtaatg cccgttcgca cattgtttga gatgaccctt ggcgaaacgc
    24601 tcttccttaa tgccaaactg ccgaccggta aagaatttat gccgcgtgaa atcagtttgt
    24661 tgattggtga agagggaaat ccgctgagca gccaggaaat cctggaaggc ggtgaatcgc
    24721 tgatattatc ggaagtcgca gagccgccag cacaaatgat tgattcactc accaccttat
    24781 ttaaaaccat taagccggtg aagcgtgctt ttatttgttc aattaaagag aacgaagagg
    24841 cacagcctaa tttacttatt ggcattgaag ccgatggtga tatcgaagaa attattcagg
    24901 cgacgggaag tgtagcgacc gatacattac ctggcgatga accaatcgat atttgtcagg
    24961 tgaaaaaagg ggaaaaagga attagccact ttattaccga acatattgcg ccattctatg
    25021 aacgtcgctg gggtggtttt ttgcgtgact ttaaacaaaa ccggataatc taaaatatgc
    25081 cgccctaaag ggcggcattc gggcttactt gctgctctga aggttgttaa ccaccagtaa
    25141 attgtcgccg gagagactcc ggttattatt tacttgggaa aacagcgcac cagaaacaat
    25201 aagtacgata cagaagcacc gtatcttcat tgtgctgtcg tgatgcgaag accgcatcac
    25261 gacagcacca ctggcgatat tgccgcgata gcaagcgctg agacgcgctt gctctaacgg
    25321 tcgcggaaca tcctttcctt aagccagcgc ccgcttgcga gacggcggaa agggccgcag
    25381 tggataatac actaacagaa caataataac tctcttttgc ttgacaaaaa gagagttact
    25441 ggcgagtatt gtctccgctg agacgcgctt gcattactac ggtaatacaa cctgctggaa
    25501 aggccgcgaa ccagaccagc aataaaaaaa cgccaaattc ggcggttttt tattgcctgt
    25561 aaaatactta tttcactggc tcaaccggta aatctgcccg cgcgccccat tcactccatg
    25621 cgccgtcgta cagtttcacg tttggcacat ccagcgtcgc gagtgctaac aaaaccacgg
    25681 ctgccgttac accagagccg cagctgacga taatcggttt gtcatagctg acgccgcgac
    25741 caaaaaatat cgcatccagt tcatcggtcg tttttagttc gccttcgcgc accagttccg
    25801 tccacggaac gttcaatgct ccgggaatat gtccgcgacg taaacctggg cgaggttcat
    25861 caacttctgc gttaaaacgt gcagccgggc gggcatcaat aatttgcgcc gtattttcat
    25921 ggcttgccaa taacacatcg gttactttca ccactgcttc aggattaaac gcggcgttaa
    25981 actctccttc cggcagctct actgcacctt cttctaatag caggtcatcg cgctgccagc
    26041 ctgcaag
//
LOCUS       DAJTNM010000045        25619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTNM010000045 DAJTNM010000000
VERSION     DAJTNM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.045"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..95)
                     /locus_tag="OO172_002002"
     CDS             complement(<1..95)
                     /locus_tag="OO172_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HCR5512201.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYA"
     gene            complement(165..1154)
                     /gene="araF"
                     /locus_tag="OO172_002003"
     CDS             complement(165..1154)
                     /gene="araF"
                     /locus_tag="OO172_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR5512202.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1951..2454
                     /locus_tag="OO172_002004"
     CDS             1951..2454
                     /locus_tag="OO172_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5512203.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2534..2785)
                     /locus_tag="OO172_002005"
     CDS             complement(2534..2785)
                     /locus_tag="OO172_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5512204.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2900..2986)
                     /gene="azuC"
                     /locus_tag="OO172_002006"
     CDS             complement(2900..2986)
                     /gene="azuC"
                     /locus_tag="OO172_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5512205.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3249..3572
                     /locus_tag="OO172_002007"
     CDS             3249..3572
                     /locus_tag="OO172_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5512206.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLH
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3743..4240
                     /gene="ftnA"
                     /locus_tag="OO172_002008"
     CDS             3743..4240
                     /gene="ftnA"
                     /locus_tag="OO172_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5512207.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4277..4516)
                     /locus_tag="OO172_002009"
     CDS             complement(4277..4516)
                     /locus_tag="OO172_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5512208.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4706..5917
                     /gene="tyrP"
                     /locus_tag="OO172_002010"
     CDS             4706..5917
                     /gene="tyrP"
                     /locus_tag="OO172_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5512209.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSMPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5979..6644)
                     /gene="yecA"
                     /locus_tag="OO172_002011"
     CDS             complement(5979..6644)
                     /gene="yecA"
                     /locus_tag="OO172_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5512210.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6840..6926)
                     /locus_tag="OO172_002012"
     tRNA            complement(6840..6926)
                     /locus_tag="OO172_002012"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6890..6892),aa:Leu,seq:taa)
     gene            complement(6939..7012)
                     /locus_tag="OO172_002013"
     tRNA            complement(6939..7012)
                     /locus_tag="OO172_002013"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6978..6980),aa:Cys,seq:gca)
     gene            complement(7294..7842)
                     /gene="pgsA"
                     /locus_tag="OO172_002014"
     CDS             complement(7294..7842)
                     /gene="pgsA"
                     /locus_tag="OO172_002014"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5512211.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7899..9731)
                     /gene="uvrC"
                     /locus_tag="OO172_002015"
     CDS             complement(7899..9731)
                     /gene="uvrC"
                     /locus_tag="OO172_002015"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5512212.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9728..10384)
                     /gene="uvrY"
                     /locus_tag="OO172_002016"
     CDS             complement(9728..10384)
                     /gene="uvrY"
                     /locus_tag="OO172_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5512213.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10680..10856
                     /locus_tag="OO172_002017"
     CDS             10680..10856
                     /locus_tag="OO172_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512214.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10843..11067
                     /locus_tag="OO172_002018"
     CDS             10843..11067
                     /locus_tag="OO172_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5512215.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11100..11720)
                     /gene="sdiA"
                     /locus_tag="OO172_002019"
                     /pseudo
     CDS             complement(11100..11720)
                     /gene="sdiA"
                     /locus_tag="OO172_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11950..12702)
                     /gene="yecC"
                     /locus_tag="OO172_002020"
     CDS             complement(11950..12702)
                     /gene="yecC"
                     /locus_tag="OO172_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5512216.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12699..13367)
                     /gene="tcyL"
                     /locus_tag="OO172_002021"
     CDS             complement(12699..13367)
                     /gene="tcyL"
                     /locus_tag="OO172_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5512217.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13382..14368)
                     /gene="dcyD"
                     /locus_tag="OO172_002022"
     CDS             complement(13382..14368)
                     /gene="dcyD"
                     /locus_tag="OO172_002022"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5512218.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14473..15273)
                     /gene="tcyJ"
                     /locus_tag="OO172_002023"
     CDS             complement(14473..15273)
                     /gene="tcyJ"
                     /locus_tag="OO172_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5512219.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15361..15912)
                     /gene="fliZ"
                     /locus_tag="OO172_002024"
     CDS             complement(15361..15912)
                     /gene="fliZ"
                     /locus_tag="OO172_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5512220.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15958..16677)
                     /gene="fliA"
                     /locus_tag="OO172_002025"
     CDS             complement(15958..16677)
                     /gene="fliA"
                     /locus_tag="OO172_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR5512221.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVCHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16842..18494)
                     /gene="fliC"
                     /locus_tag="OO172_002026"
     CDS             complement(16842..18494)
                     /gene="fliC"
                     /locus_tag="OO172_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5512222.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTVGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18760..20166
                     /gene="fliD"
                     /locus_tag="OO172_002027"
     CDS             18760..20166
                     /gene="fliD"
                     /locus_tag="OO172_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5512223.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20191..20601
                     /gene="fliS"
                     /locus_tag="OO172_002028"
     CDS             20191..20601
                     /gene="fliS"
                     /locus_tag="OO172_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5512224.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20601..20966
                     /gene="fliT"
                     /locus_tag="OO172_002029"
     CDS             20601..20966
                     /gene="fliT"
                     /locus_tag="OO172_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5512225.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     REIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21044..22531
                     /gene="amyA"
                     /locus_tag="OO172_002030"
     CDS             21044..22531
                     /gene="amyA"
                     /locus_tag="OO172_002030"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5512226.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDCIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22565..22978)
                     /locus_tag="OO172_002031"
     CDS             complement(22565..22978)
                     /locus_tag="OO172_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5512227.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23165..24370
                     /gene="yedE"
                     /locus_tag="OO172_002032"
     CDS             23165..24370
                     /gene="yedE"
                     /locus_tag="OO172_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5512228.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24367..24600
                     /gene="yedF"
                     /locus_tag="OO172_002033"
     CDS             24367..24600
                     /gene="yedF"
                     /locus_tag="OO172_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5512229.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24707..25377
                     /locus_tag="OO172_002034"
                     /pseudo
     CDS             24707..25377
                     /locus_tag="OO172_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25417..>25619)
                     /locus_tag="OO172_002035"
     CDS             complement(25417..>25619)
                     /locus_tag="OO172_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5512230.1"
                     /translation="CGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLAC
                     GEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         6369 a         6542 c         6089 g         6619 t
ORIGIN      
        1 gcatagcagt caaaactaat atccgtcagc gccttaacgc cgggaaacgt tttaccgatg
       61 ccgcgaaatg agagatacgg ggtagactgt tgcataacgt ctccgtgaat cactggtcgt
      121 tcacaccccc tctgcatcat gcagagggga atttttccgg caaattactt accgcctaaa
      181 ccttttttct ccagttcttc tttaaagttg tcacgcgtga tcagtaccac gtctgtaact
      241 tcggtaaatt ttggcgtttc aacgtctttt gctacccagt tgtaaagcat ttcgctggat
      301 ttatagccat gtacgtccgg gcttggcagc agggaaccgt agaagccggt tgcctgtgct
      361 ttagacagtt cgcttactgc atccacaccg ttaataccaa taccgatgat atcggctgct
      421 ttaaagccct gaccttcggt tgcacgtacg ccgccaagta cggtgctgtc gttcataccg
      481 acgatcagcc aatgtttaac ttccggatgt tgaaccagca ttgagttggc agcgtcaaat
      541 gcccccggga tgtcgttaga tttggtaggt acctgataaa tttgtttttc cgggaatcct
      601 gcagctttca gtgcgtccat agaacccgtt gtacggcggc gggcggtatc cagttcgttg
      661 gcggtaatcg ccatcactgc gctttctttg acatcccagc cacgtttctg catctcttta
      721 tacagttcct ggccctgacg ttcgccaatt ttagtcgccg ccatcatcac cagaggaacg
      781 gtatccattg gcttaccttt ggcgttaaca aactggtcat ccactgcaat gactttcata
      841 tcgtagccac gcgctttcgc gacgatggca gagccgagtt tcgggtccgg agtacaaata
      901 acgaaacctt ttgcgccact ggctgccagg ctgtcgatcg cgttcaatgt tttttcgcca
      961 tccggcacgg caatcttaat aacctcaaac cctaaatcct tcccggcttt atcggcaaac
     1021 ttccattcgg tctggaacca cggctcttcc ggttgcttca ccagaaaacc gagcttcagg
     1081 ttctccgcca tagcggattg tgacataacg gctgccagac caatggctgc cagggcttta
     1141 gtaaatttgt gcatggttct ctccagcttt agtgtcgttt tgtgtagggc aaaaacgaat
     1201 gacattcgtt aaattaatcg gaaaacaaag cattaccttt tatctaaaag ataagtgacc
     1261 gtgttgacat agttttagcg agaaattaat tctccatagg agagcaatat cacatcgcag
     1321 aattacagtg agaacgtgca taaatttagc gggaaaagac ataaggaaaa gccaatttgt
     1381 cagacaaatt gttgaatgca cagcagatta atccataaga ttagcctgga aatccttgtt
     1441 gtcttcggta cccatgcggg atgtcttctt tttaaccagt caataggccg cattacctgg
     1501 cgttgagttt ttgaaatggt gtaataacca caactcaaag atgtggaaaa tgcgcgtcat
     1561 tcatttcgcc attaattatc actgtgctca ttaattaaca gaacacgtat aatgagagcc
     1621 atctcgcaaa aatgaaaaaa cgttttataa aatcatcact tcatcatgaa ttcaaattca
     1681 tcgattaata tcaacaagat acaaaaagca ctatcattaa aattcattgc atttacattg
     1741 atttcaccaa tgaaatgtaa aaatatataa acttgatgat ttaagcattt tcttataacc
     1801 gttcagacgt tattcttatt tcagatcatc gtcagaattg actccacgat cacatttcgg
     1861 accggcagaa aggaattatt ctgcaaacag taattatggt gttttgattt atcttgcacc
     1921 tctccacttc tggatataag gatattaggt atggcaaccg ctggaatgct tctcaaactc
     1981 aactctcaaa tgaaccgcga gttttacgca tccaatctct accttcacct gagtaactgg
     2041 tgttctgaac agagtctgaa cggcaccgcc actttccttc gcgcccaggc acagagtaat
     2101 gtgacccaaa tgatgcgcat gtttaacttt atgaagagtg tcggcgctac ccccatcgtt
     2161 aaagccattg atgttccagg tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg
     2221 atggaagaat acgagcaacg ttcaagcacg ctggcgcagt tagccgatga agcgaaagaa
     2281 ctgaatgatg attcaaccgt caatttcctg cgcgatctgg aaaaagaaca gcagcatgat
     2341 ggcctgttgc tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc
     2401 cctgtgcaga ccgaccaaca tgttctgaat gtcgtgtcac accagctgca ttgatcatac
     2461 atcggcgcta atacattgcg ccgatgaagt ttttgagaaa ccgctgcctc atctgtttga
     2521 agcagcggtt tttttaatgg gattcaccct gtggggtaaa cttgagttca atcagcgcga
     2581 tggctttttg gattgcccgc atggtgaccg ggtctgcggc agcgggatga tgagtaaagt
     2641 cgatattctt cagctgactg gacatttttt cacgaacttc aacgggcgcg attacatcga
     2701 gaacatccag aatttgtttg ataaccaact ggcaagctac cacatcagaa accagttcct
     2761 gatcggcatt cagcggctgg gacatcgtaa actcctgata gcattttgaa agccgttata
     2821 gtagcggctt cacatcttca gcgatagtca catccaccgt catcaggaca caaaaaaacc
     2881 tgccggagca ggttttttgt tatcggaaca tattgcctgg cggtacgtct ttgaacgtct
     2941 tgcaatagtt attgaacata cttttcagga ttttgcgcag tttcatcgcg gcactccgac
     3001 catttgttat acaggtgtta ttgtctttgc gcttataata tgacaaccat cacaaaaatc
     3061 aatctttatg tgatacaaat cacataaata cccctttaat gttataaaaa tgataatcaa
     3121 aaaacagccc ccctatttct gacacctaca gatggcaaga aatagcgcct gccaggcatc
     3181 ttttccggcc attgtcgcag cgctgtaacg cgtaaaatag tgctttctct tactcttctg
     3241 gctggaccat gagacttctg attctgactc tttcattaat aacgctggcg gggtgtacgg
     3301 ttactcgcca ggcccatgtg agcgaagttg acgcggcaac aggtattgta cggttggttt
     3361 atgaccaggc ttttttgcat catgcccata ccgatcgtta tgtcagtcgc gggatcgccg
     3421 atcgcgcatg ccagcaggaa ggctataccc acgcgatccc ctttggtcag ccagtaggca
     3481 actgtagcct ttttgcgggt tccttatgtt tgaatacgga attcactttg tcatatcagt
     3541 gccatcattc cgccttccct gtctttctct aaatatttta atcagcgagg ggatcttcgc
     3601 tgattaaaga aatagcggta atgcgtttta ttcccattcg tatttttaat aattgaagtt
     3661 tatattttac cttttgcaaa taataaaata acaaattata gtgacgccac agcaacaaat
     3721 ataacctttg tggagcacta tcatgctgaa accagaaatg attgaaaaac ttaatgagca
     3781 gatgaacctg gaactgtact cttcactgct ttatcagcaa atgagcgcct ggtgcagcta
     3841 tcataccttc gaaggtgctg ccgcgttcct gcgccgtcac gcccaggaag agatgacgca
     3901 tatgcagcgt ctgtttgatt acctgactga taccggcaat ttaccgcgta ttaataccgt
     3961 tgaatctccg tttgctgaat attcctcact tgatgaatta ttccaggaaa cctataaaca
     4021 cgaacaatta atcacccaga aaattaacga actggctcat gctgcaatga ccaatcagga
     4081 ctacccaaca tttaatttcc tgcagtggta tgtttctgag cagcatgaag aagagaaact
     4141 gttcaaatcg attattgata aattaagcct ggcaggcaaa agcggcgaag gtctgtattt
     4201 tatcgacaaa gaactctcta ccctcgacac acaaaactaa tgctctccgg cggcaggttt
     4261 ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg atcggtcgaa aaaccttctt
     4321 ctgaaggtat aaatttgcct tttgctgcca gaaacgctac cagctctccc gctgtcatcc
     4381 cttctgccga gcacgtgtga aaacgtgcct gttcaccaaa acgcgcttta atcgcagctt
     4441 ccagactggc atgcgtatat tgctcgcctg attcaatcat catatttaac acttcatgac
     4501 cgtgaataga gtccatcgtt cctcctcaaa aaaagcctag cgtagcgatt accgcttatg
     4561 aagactttgc gccagcgcag gactgaatgc ttttattgta catttatatt tacacaatat
     4621 gtaacgtcga tttgacgaag cagccgttat gccttaacct gcgccgcaga tatcactcat
     4681 aaagatcgtc aggacagaag aaagcgtgaa aaacagaacc ctgggaagtg tttttatcgt
     4741 ggcgggaacc acaattggcg caggcatgct ggcaatgccg ctggctgcgg ccggtgttgg
     4801 ttttagcgtt acgttaatct tgttgattgg gctttgggcg ttgatgtgct acacggcgct
     4861 attactgctg gaggtgtacc agcatgttcc ggcagatacc ggtctgggca cgctggcaaa
     4921 acgctatctg ggacgctacg gtcaatggct gacgggcttc agtatgatgt tcttaatgta
     4981 tgctctgact gcagcataca tcagcggtgc cggtgaattg ttggcctcca gcatcagcga
     5041 ctggacaggt atttcaatgt cggcaaccgc tggcgtgctg ttgttcactt ttgttgccgg
     5101 tggcgtggtt tgtgttggaa catcgctggt cgatttattt aaccgttttc tgttcagcgc
     5161 caaaattatt tttctggtgg tgatgctggt attgctgcta ccgcatattc acaaagtgaa
     5221 tcttttaacc ctgccgttgc aacaggggct ggcactgtct gcaatcccgg tgatttttac
     5281 gtcgtttggt tttcacggta gcatgccgag tattgtcagc tatatggatg gcaacattcg
     5341 taagctacgc tgggtgttta taatcggtag tgccatcccc ctggtggcat atattttctg
     5401 gcaggtggcg acgcttggca gcattgattc aacaaccttt atgggattgc tggctaatca
     5461 tgctggatta aacgggctgt tacaggcgtt acgcgaaatg gtgccctctc cgcatgttga
     5521 gctggcggtg catttatttg ctgatttagc cctcgccacg tcatttctcg gcgttgcgtt
     5581 aggcttattt gattatctgg ctgatttgtt tcagcgttca aataccgttg gtggacgatt
     5641 gcaaactggt gcaattacct ttctgccgcc gttggcgttt gcactgtttt atccacgagg
     5701 atttgtgatg gcgctgggtt acgccggtgt ggcgctggcg gtactggcat tgattatccc
     5761 ttcactattg acctggcaaa gcagaaagca caatcctcag gcgggttacc gggtcaaagg
     5821 tggtcgtccg gcgctggtgg tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt
     5881 tttgattgcg gcagggttgt taccagaagt ggggtgatca gatagcctca aattccttat
     5941 tgggtgccag aattaacgct gacacccaat gtgacctctt aatgcaggca gcactgctta
     6001 aatttcttac cactgccgca cggacaagga tcgttacgcc ccggtttctc ttctgctttg
     6061 atcggttgct gaacagcttt ttcctgcgga tgcgccatcc agtatgcatg taaatcaagc
     6121 gccgccagtc gaatggcatc tacgctctct tcaaacgctt ctggcgacat tttctctacc
     6181 cgctcgaagt tttcctcagt accgtgcagc gcaatcgcct ccagcgctgg ttttaaagaa
     6241 tcgggcaacg ttgaccagtc agaaagtgcc acgccccgca tatagccaaa gcaccactcc
     6301 tcaacaatcg tcagctcgct gccatcaact tctcgcaagc cgaataacgg ctcaaactgc
     6361 tccgggaatt cgttcagacg ctctgcggta tcggccatat gttgaaaagc caggttcata
     6421 aagcgcgtca tctctttctc tgacgcccaa cgcggcacat agtcagcccc accccacacg
     6481 gcaaccagcc actgttctgg ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa
     6541 ccgtccagct ccgccacatc aaggatggcg tggtcagtgt tgtatttggt cagaatatcg
     6601 tccagccatt ccaactcact ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg
     6661 atctcaactc gccagcacct atcttacatg ccggtccgta tcagagatac tttttgagtg
     6721 gctttgccgg tgattaaaaa ttaaggaggg tgtaacgaca agttgcaggc acaaaaaaac
     6781 cacccgaagg tggtttcacg acactgctta ttactttgat tttattctta tctttcccat
     6841 ggtacccgga gcgggacttg aacccgcaca gcgcgaacgc cgagggattt taaatccctt
     6901 gtgtctaccg attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt ccggagtcga
     6961 accggactag acggatttgc aatccgctac ataaccgctt tgttaacgcg ccaaattctt
     7021 caggcctttc agccagacat ccgcttgacg ccgatgtctt ttaaactgga gcgggaaacg
     7081 agactcgaac tcgagacccc gaccttggca aggtcatgct ctaccaactg agctattccc
     7141 gtattcatca agcaatcagt taatcacttg attttattat cgtctggcaa tcagtgccgc
     7201 cgttcgatgc gttgcattct acttacctgg cgcgatgagt caacgatatt tttcaccact
     7261 tttgatcgtt tgctgaaaat tacgccgaaa cgatcactga tcaagcaaat ctgcacgcgc
     7321 agcgctcaaa tattgcaaca ttgaccacag agtcagtacc gcagccacaa agaaaagtgc
     7381 aataccggcg tactcaaccc aaatgttcgg acgccacagc agccaggcca acgccaccat
     7441 ctgggcagtg gttttcactt tcccaatcca ggagacggcc acgctactgc gtttacccaa
     7501 ctccgccatc cattcgcgta gcgcagaaat aataatttca cgggcgatca tcgttgccgc
     7561 cggtaatgtc acccaccagc tgtgataatg ctcggttacc agcaccatgg cgatagccac
     7621 gagaacttta tctgccacag ggtcaaggaa agcaccaaac cgggtactct ggttccagcg
     7681 gcgtgccaga aaaccatcga accagtcagt caccgccgcg acgcagaaaa tgagcgcggc
     7741 ggcaaacggc gaccaggtga caggcagata aaagaccaat acaaagaatg ggataaggat
     7801 gacacggaac agtgtaagca acgtagggat attaaattgc ataatgacgg gtaactatct
     7861 gttgtcagta agattacccc tatgttgcta cagagacatc aatgtttcaa cgaccagaag
     7921 atcttttctg ccagaccttg cgaaataccc ggcacttttg caatttcctc gacgctggcg
     7981 ttacgtaaac cttgcaaacc gcccatatat ttcaacaaca tttgccgacg ttttggcccg
     8041 acgccttcaa tggtttccag ggaactggta tttttgacct tcgcccgttt tttacggtgc
     8101 ccgccaatcg cgtgatcatg tgattcatcg cgaatatgct ggataacatg cagcgcgggt
     8161 gaatctggcg gcaaactaaa tccctcacct tccggctcaa agaacagcgt ttccagtcca
     8221 gccttacgat ctgctccttt ggcaacgcca agtagcagcg gatgattttt atcccatgag
     8281 acatccagtt cggcgaagac atttttcgcc tgcgcaagct ggcctttgcc gccgtcgata
     8341 aggatcacat ccgggatctt actgtcgtca atggctttac cataacgccg acgcagcacc
     8401 tgattcatcg ccgcataatc atcgcccggc gtgatgcctg taatgttata gcgccgatac
     8461 tccgcacgca gcgggccgtt agcatcaaac accacacagg aagcgacggt ttgttcgccc
     8521 atggtatggc tgatgtcaaa gcactccatc cgcttcactt ccggcaattt caacacgctg
     8581 gcaagcgcgg tcagtcgctg gtgaacggta gattgctgcg aaagtttgct ggttaaggcc
     8641 gtcgccgcat tggtgcgcgc gagtttcaga taacgcgccc tatcgccgcg aggtttggtt
     8701 tgaacattaa tcttgcgtcc cgccagttct gaaagggaat cggcgagcag cgttttatcg
     8761 ctaagattaa aatcgagcag gatctcaccc ggtaaggtgc gcatctggct gccttgtaaa
     8821 tagaactgac ctacgaaggt ttctaccacc tcgctcagtt ccgtaccgcc aggcactttc
     8881 gggaaatagc tgcggctgcc gagcactttg ccctgacgaa tgaacaatac gtggacacaa
     8941 gccatgcccg catcgaacgc cacaccaata acgtcaaggt cgtcgccggt attggaaaca
     9001 aactgttttt cggtgacgcg tcgcaccgcc tgaatttggt cgcgaataca tgccgcttct
     9061 tcaaactcca gattctggct ggcagtttcc atacggctaa tgagttgcgt aagcacctga
     9121 tcatctttgc cagacaaaaa caggcgcaca tactcgacct gctgagcgta ttcttcttca
     9181 ctcaccagtc cttcaacgca cggtcccaga cagcgcccta tctggtattg cagacacgga
     9241 cgcgagcgat tgcgataaac actattttcg cactggcgaa tggggaaaat cttttgcagt
     9301 agcgccagtg tttcacgtac ggcatagcca ttcgggaacg ggccgaaata ttcacctttg
     9361 gcatgctttg caccacgatg catcgccaga cgcgggtggg tatcgccact caggaagata
     9421 aaaggataag atttatcatc gcgtagcaaa acgttgtaac gcggctgata gagtttgatg
     9481 tagttgtgtt ccagcaacag cgcttcggtt tctgtatgcg taaccgttac atcaatttgc
     9541 tggatctggg cgaccagcgc ttcggttttg cgcgaagcga ggttgctgcg gaaatagctg
     9601 gaaagccgtt ttttcaggtc tttcgctttg ccgacataga taaccgtacc accagcatcg
     9661 tacatgcgat aaacgcctgg ctggctggtt acggttttta aaaacgcttt tgcgtcaaac
     9721 tgatcactca ctgacttgat aatgtctccg cattacacag accatggcga attgccaggt
     9781 gagtcagctc aacatcgcca tgaatgttta gtttactgaa catacgatag cggtagctgt
     9841 tcaccgtttt cggactgaga ttgagctgtt ctgagatctc attgaccttc tggcccttag
     9901 tgatcatcag cataatctgc aattcacgtt cagacaaact ggcaaatggg ctttctgttt
     9961 tttctggttc gatctggctt aacgccattt gttgagcgat atcagaagca atgtaacgct
    10021 gccctgaata gacagaacga atcgcactca cgacttcctg cggagccgcg cctttgctga
    10081 ggtagccagc agcacctgcc tgcatgactt tcgctggtaa agggttttct gtatggaccg
    10141 taagcatgat gattttaaca tcagctgtgg aacgcgcgat tttacgcgtc gcctcaagac
    10201 cgccaatgcc cggcatactc atgtccatta gcaccacgtc aacggcattt gtccggcacc
    10261 acttaacggc gtcttcaccg cacgatgcct ctccgacgac ttttataccc tttatatctt
    10321 ccagaatgcg tcgtatccct gcgcgcacca gttcgtggtc atcaacaagt agaacgttga
    10381 tcaaaggaat atctccagaa atagggataa cgctactgat agttagtcat tcgtatatta
    10441 gcggttttta ttgcaacttt gaaacgttaa aaatggtggg tattcgattt ttctctcgtt
    10501 tttgtcaatt cgattgtcca cagttatggt cacgcccgtc accaacggct taccggatcg
    10561 tttcacagta agccctacga tgaaatgtga caaaaattga ctttattcag caaaaatgaa
    10621 aatcagcccg ctgaatatgt aacattaatt aacccgaaca atacgggcaa aaaacattca
    10681 tgataaaaat atttattggt cattatatta acgtatttta tagcactgcc gatatcacgc
    10741 tcaaaaaaca accactgcta tttttagcaa agcttatggt atactccgcc gccttaacat
    10801 ttttcaccgc aaattttcat tgcaacatga cgaggaaaat aaatgagtac gcctgatttt
    10861 tctactgccg agaataatca agaactggca aatgaagtct cctgcctgaa agcgatgctg
    10921 acgctgatgc tgcaggcgat gggacaagct gacgcgggcc gcgtgatgtt aaagatggaa
    10981 aaacagcttg cgctgatcga agacgaaacc caggctgcag tattttccaa aacggttaag
    11041 caaattaaac aagcctaccg tcagtaatat aaaaccggct gagagcgtgc ctttcagccg
    11101 gttattgaca aaatcatcgc tatctctgct gatgtcttcc cttccgccgt ccatttcaga
    11161 atttcttttt cgcgcttgct gaaattcatc tctggcgtca tcactatttc atcatttaaa
    11221 cgcatcagag ccatcagact ttcgcgcacc agtaactgca tttttaattg cagttcatca
    11281 ctaagaatgg gtatttcgcg cgtgctgcaa cgggaaaagg acaaaaagcc cagcgcccga
    11341 tttggcagca ttaaatactg agtgacaccg cggcgtaaac catgtgcgtg cgcggcttcc
    11401 cataatggct gtgcttcgct gaataagtca tcattccaca ttaaatggtc ctgactaaag
    11461 ttttcagggt tcagcacagg atcaattgcg agaaaatttt ttgcctgata ataactaacc
    11521 cacgactcag ggtaattggt gtagaaagcc actttaggtc gcgtgaatag taccgggtgg
    11581 cgaacacata acgaatagta atcgtactcc agctgctgag cctgaagctc tatttcatgg
    11641 tagacctctt ctgcggcctc catcctctga aaacgcaaca gcatcgtgcg acgccagctg
    11701 aaaaaatccg tatcctgcat agtaaaccgc aacgcccctg agagtgagta tcatttataa
    11761 tgataattca aactatcaca taaataagat ttatatataa tttatattat tcaggcaatg
    11821 aattactttt gcaagccatc gcattctctt atgttattaa tgagttatgc tgatttgtta
    11881 agcagtttta tcacgcttga aatggcgtcc cgccccgaca ggtgaatcgt cggggctgat
    11941 tttttcttat tattgcagca gaagcttctc gaggaactgg cgggtgcgag gctgctgggg
    12001 atcggcaaat aacgcttttg ctgccccctg ctcgactatc cgcccctggt ccataaagat
    12061 cgcccggtcc gcaacatccc gggcaaagct catttcgtgt gtcacaatca ccatcgtgcg
    12121 cttttcctgc gccagctggc ggatggtgtt caggacttcg cccaccagct ctggatccag
    12181 cgccgacgtt ggctcgtcaa acaaaatcac ctcaggacgc attgccagtg cacgcgcaat
    12241 cgcaacacgc tgctgttgac cgccagacaa acgacgtgga tagctggttt ctttaccggc
    12301 cagcccaact ttcgccagca gctcgcgagc gcgcgccgtg gcctcttctt tcggttcgcc
    12361 tttgacgatc accggccctt caataatgtt ttccagcacc gtacgatgcg gaaacaaatt
    12421 aaagttctgg aagacaaacc cgacgtgctg acgcaactgg cgaatcagcg atttttgctg
    12481 acttaatgaa cgtgcagtat caatagtgat atcgccaacg gtgatcgttc ccgcttccgg
    12541 ttgttccagc agatttatgc tacgtagcaa tgtggttttg ccggaaccac tcggaccgat
    12601 aattgccacc acttcgccag gctttacctc gaggtcgata ccgtgcagca ccgtctgacc
    12661 gtggaatttt ttcaccaggt tcttaacttc aatggcactc attttggttc tctctcctgg
    12721 cgattaagtt gattctcaaa atggttctgc aacgtcgata acacagtcgc catgatccag
    12781 tagatgagcg aagccgccag gtacatggtg aacacctcca gcgtgcgcga ggtaatcaac
    12841 tgcgcctgac ggaacagctc cggcacctgg attgtcgcgg ccagcgacgt atcttttacc
    12901 agactgataa agctgttcga cagcggcggc aacgccaccc gcgccgcctg tggcaaaatc
    12961 gcgcgacgca tggtctgcca cggcgtcata ccaatactgg cagcggcttc ccactgacct
    13021 ttatcaatag aagaaatggc ggcgcgcagc gtttcggcgg cataggcggc agtgttcagc
    13081 gacagaccaa tcattgctga cggaatggga tctaattcga taccaaactg cggcaggccg
    13141 taatagatca taaacagttg tgcgatgagc ggcgtaccac gaaaaataga gatataaaaa
    13201 cgcgccagcc agcgcacggg ccagattggc gacaggcgca tcagcgcgag aataaacccg
    13261 agtagtaagc caaaaaacat gccgccgata ctgagttgca gggtataccc agcccctttc
    13321 aacaggaacg gcaaggaatc aataaccagt tgtatacttt cttgcatgag cgttcccgga
    13381 gttaaacgtg gggatgatag gcgaacagcg caggcgcgcc gccggtatga ataaacagaa
    13441 tcggcccttc atctttgaag cgtttctgac tgataccgtc aatcagcccc gccatcgctt
    13501 ttccggtata cacaggatca agcagaatac cttccagacg cgccagcaat ttcactgctt
    13561 ccatgccttc gtcgttcggc acgccgtagc caggtgcaaa atagtcatcc cagagtaaaa
    13621 tttccgctga tgcggtcagc tccagttctt tcgcaatcgc ctgttgtagg ttaaccactt
    13681 tcggcaattg atcggcaacg gaacgcgaca cggtcacgcc aatcagttcg ctttcaggca
    13741 tcaggtgttc cagcccaaca gccagtccgg cgtgagttcc ggcactgccc gatgcgacta
    13801 ccaccgacga aatattaacc gccccttcac actgttgcgc gatttccagc gcactctcca
    13861 cataacctag cgcgccaaga gcattagaac cgccaaccgg aatgacatac gggcgaaagc
    13921 cttgtgcttc gactcgcgtc gccagctctt ccagttgggc attgggatcg gtcagtgcgt
    13981 cgcacatttc aatctgggta ttgaacagat ccagcaacaa acgattgccg ttggttaaat
    14041 agttttctgc ggttgtgcca ataggatttt ccagcagcgc cacgcagtgc agaccgagtt
    14101 tcgccgcgac tgcggcagtc tggcgcacat ggttagactg gatcgcgccg gcagtaatca
    14161 gcgtatcggc accttcgcgc agggcatctg ccgcgagaaa ttccagctta cgtaatttat
    14221 tgccgcccat tgccatgggg gtgacctcat cccgtttgat gaaaatttcc cgtccaagat
    14281 aatcagaaaa gcgcggcaga tattcgagcg gcgttggcgc gccgataaac tccaaccgtg
    14341 gaaaacgggt taaattatgc agtggcataa cagcctccga tgcgtgttgt tgtgattttc
    14401 ttattatgca cgctgaaaac gcgtaaataa aaaaggcgct agtgaaagcg cccttttttg
    14461 tcattatgct gattatttgg tcacatcagc accaaaccat ttttcggaaa gagcttgcag
    14521 agtgccgtct ttttgcattt ccgcaattgc atcattcacc gctttcagca ggtcttcatt
    14581 tcctttacgc agcgccacgc cagactcctg acgggagaat gcttcaccgg ttactgccag
    14641 cgtatcgttg gttttcttca ccagatccag cgccgccaga cgatcaacga ggatcgcatc
    14701 gatacgccct acgcgcagat cctgatattt ggtcgggtca tcatcatagg tacgcacatc
    14761 gacaccctga acattctgcc gcagccactc ttcatagttg gtgcccagac cgacacccac
    14821 ttttttgcct ttcagatcag cagccgtttt aatggtgcct tcgttacctt ttttcaccag
    14881 cgcctgaata ccggaaatgg tgtaaggggt agagaaatcg tattttttct tgcgctcgtc
    14941 agaaatggtg acctgattaa tcaccacatc aatgcgttta gagtccagcg acgccagcat
    15001 accgtcccat ttggtcggtt ttagtgacgc ctcaacgcca agatgttttg ccagctgttg
    15061 agcaaattcc acttcaaaac cggttaattt gccgtcatcc ccctgaaaac tgaacggcgg
    15121 ataagtgcct tccagcccta ccagcagcgt gccgcgctct ttgactttat taagcagacc
    15181 ttcatctgca aaacttttaa cgctcatgcc cgcaaccagc gccacgccca tcacacccat
    15241 caatgcctga cgtcccagat gtgctaattt catattcacc ccgaatgttg ttatgtctgt
    15301 ttgcagtgta gagccattgt ttgtaaacac aaaaacaact ccgctacatc ttattcttat
    15361 ttaatatata tcagaagcag gcaggctgga ggatttctgc acaagtccgg tacaggtttg
    15421 gcgctgatag tgttgatact tccgtaatgc gatgcggtaa ttgttggtgc tggtggtggg
    15481 cagccacggg gcgaggattt catcaaggaa accgtcctgc agctgatcga gggaaatatt
    15541 ttgctcatgt agatgattac ccaaacggcg cagacgaacg acatattcac gcaccgtacc
    15601 tgggctcatt tcagtttgct caaaaagaaa tttcttaaag ccgataatat cgaaaaaatc
    15661 actctgcgtt ttgcaatgta aatcaccgca aaatcggcac aatgccgccc atgattgttg
    15721 ttccgtttgc cagccttttt catcaagcag cgtgtccagg cgggaaatct cgattttatt
    15781 cactattttg ccgtcgcgaa ctaaggtaat gcgatcgagt aatttacggc aatgcgcgca
    15841 atgggtctgg ctgtgtttaa agtctttaag atagcggctt aatggccgtc ttttcaggtg
    15901 ctgcaccatc attaagaact cctggtagtc aaagttaaag tgcggcattt actgacgtta
    15961 taacttaccc agtttagtgc gtaaccgttt aatggcctgg ctgtgtaact gactgacccg
    16021 cgattccccg acctccagca ccgcgccaat ctctttgaga ttcagctctt cctggtaata
    16081 gagggttaat accagttttt cgcgctccgg caacgtttcg atggcttcca tcacccgctg
    16141 gcgcagatta ctgtccagca gttgttgtag cgggttttct cgctgatgat catcagtaac
    16201 cagttcgatg ctatcgccgt gctcttcacg ccactcatcg taggagaaga gctggctgtt
    16261 attggtgtcg agcagcattt gacgataatc ggcaatatcg atccctaaac gttccgctac
    16321 ctcagtttcc gtggcgttgc ggccaagttc ctgctccagt tgccctattg cctgtgccac
    16381 ttcacgcgcg ttgcgtcgca cgctgcgcgg cacccagtca cggctgcgaa gttcatccag
    16441 catagcgcca cggatacgct gcactgcgta agttgtaaat gccgttcctt gtagggcgtc
    16501 atagcgttcg acagcattaa gtaacccaat gccgcccgcc tgtagcagat cgtcaagttc
    16561 cacgctcgcg ggcagtcgaa cctgcaggcg caatgcttcg tgacacacca gcgggacata
    16621 acgctgccac agcgagtgtt tatccattac accttcagcg gtatagagtg aattcacgat
    16681 aaacagccct gcgttatatg agttatcggc atgattatcc gtttctgcag ggtttttaat
    16741 cggacgatta gtgggtgaaa tgaggggtta tttgggggtt acgcaaaaag ccgaatgtaa
    16801 aaaaccccgt cggtggcggg gtttgagagg cattcagacg attaaccctg cagcagagac
    16861 agaacctgct gcggtacctg gttggctttt gccagcacgg agttaccggc ctgcaggata
    16921 atctgcgctt tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg aatacgggac
    16981 tgcgcttcag acaggttggt agtggtgttg ttcaggttgg tgactgcgga atccaaacgg
    17041 ttttgcaccg cacccaggga ggagcggaat ttgtcgatgg agctgatggc ttcatccaga
    17101 gctttcaggg gatcggctgt cgtagctgct tttgtttcag catctgtcgt caatttacca
    17161 tcagcatctt tataaactgc cttaccgctg ccgttagtca ctgaaccatc atcctgtagg
    17221 taaaaatcta cagtatcaga tgtggtcaac gcctcgttgt tatcaacata aaccgaatca
    17281 acaccttttg ttacagcgcc agttgtgccg ttgacgctat aacctacagt agtgaaatca
    17341 acattgtttc ccgaggttga tacagcttta tcgaaagctg cctgatcaac ttttgcacct
    17401 gttactgaat aagtaatagt gtccggcgta gtactattac cggtaaagga gatagaggta
    17461 ccgccaatgt caatggttgt cgctttggta ttattggctg caagtgtgga cagactagcc
    17521 tcagtcaaag aagcaccaga gccgtttttc gttaagcgcc cagttgtatc aatgtagagt
    17581 ttatctccat tgcttgccgt aattttacca tcactgctga tctgaacatc ctgtgcagaa
    17641 ccatcgatag taatagtgcc cttagcggta tcaccaactc ccggggtcaa atatttctga
    17701 acatcggcag ctgaagccgt tgtggtatca aaagagtatg acttacttgc actgtcatac
    17761 ttgtaattcg ttgcactagc cgccgttccg aagccgttat taatggttgc ctgaacagta
    17821 tcaccatcac taactccagc cagcaaatca gacgctttag cagcatcgta acccgcactc
    17881 acagtatatt tgttgccgac cactgttgca ttagcagcta ccaagtcagc tttagatgca
    17941 gcagtattag ccacagcccc gccaccattc acattaaacc cattcagccc cagcgtatca
    18001 gagtcaattt tcttcagatc aatagtgata gtctggccgt cattcgcacc aacctgaatt
    18061 ttcatcgaac cgtcttttgc cagtacgttc acgccgttga actgggtctg accggatacg
    18121 cggtcaattt cgtcgagacg ggatttgatt tcgtcctgaa tggagtccag atccgaatca
    18181 gagttagtcc cggtagaagc ctgaaccgtc agctcacgga tacgctgtaa gttgttgttg
    18241 atttcggaca gcgcgccttc agtggtctgt gcaacagaaa taccgtcgtt ggcgttacgt
    18301 gcagcctgag tcaggccttt aatgttagaa gtaaaacggt tagcaatcgc ctgacccgcg
    18361 gcgtcatcct tcgcgctgtt aatacgcaag ccagaagaca gacgctcgat agaactcgac
    18421 agcgcagact ggttcttgtt gatattattt tgagtgatca gcgagaggct gttggtatta
    18481 atgacttgtg ccatgattcg ttatcctata ttgcaagtcg ttgattacgt tttgggtttc
    18541 cacccgtcgg ctcaatcgcc gtcaaccctg ttatcgtctg tcgtaaaaca acctttagaa
    18601 tttttttcac aaacagccat tttttgttag tcgacgaaat actcttttct ctgcccctta
    18661 ttcccgctat taaaaaaaac aattaaacgt aaactttgcg caattcaggc cgataacccc
    18721 ggtattcgtt ttacgtgtcg aaagataaaa ggaaatcgca tggcaagtat ttcatcgctg
    18781 ggagtcgggt caggtctgga tttaagttca atccttgata gcctcaccgc cgcgcaaaaa
    18841 gcgacgctaa cccctatttc aaatcagcaa tcgtcgttta ccgctaaact tagcgcctac
    18901 ggtacgctga aaagcgcgct gacgactttc cagaccgcca atactgcatt gtctaaagcc
    18961 gatctttttt ccgccaccag caccaccagc agcaccaccg cgttcagtgc caccaccgcg
    19021 ggtaacgcca tcgccgggaa atacaccatc agcgtcaccc atctggcgca ggcgcaaacc
    19081 ctgaccacgc gcaccaccag agatgatacg aaaacggcga tcgccaccag cgacagcaaa
    19141 ctcaccattc aacaaggcgg cgacaaagat ccgattacca ttgatatcag cgcggctaac
    19201 tcatcgttaa gcgggatccg tgatgccatc aacaacgcaa aagcgggcgt aagtgcgagc
    19261 atcattaacg tgggtaacgg tgaatatcgt ctgtcagtca catcaaatga caccggcctt
    19321 gataatgcga tgacactctc ggtcagcggt gatgatgcgc tacaaagttt tatgggctat
    19381 gacgccagtg ccagcagcaa cggtatggag gtctcggtcg ccgcccagaa tgcgcagctg
    19441 acggtcaaca acgtcgccat cgagaacagc agcaacacta tcagcgacgc gctggaaaac
    19501 atcaccctga acctgaacga tgtcaccacg ggcaaccaga cgctaaccat cactcaggac
    19561 acctccaaag cgcaaacggc gattaaagac tgggtgaatg cctataactc gctaatagat
    19621 accttcagca gcctgaccaa atacaccgcc gtagatgcgg gagctgatag ccagagttct
    19681 agcaatggcg cactgctcgg cgactccacg ctgcggacga ttcagacgca gttgaagtcg
    19741 atgctgagta ataccgtcag ttcttccagc tataaaacgc tggcgcagat tggtatcacg
    19801 accgatccca gcgatggcaa actggaactg gatgccgaca aactcaccgc tgcactgaaa
    19861 aaagatgcca gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac cggcatcaca
    19921 accaccatcg gcagcaacct gaccagttgg ctttcgacaa cgggcattat taaagccgct
    19981 accgatggcg ttagtaaaac cctgaataag ttaactaaag actacaacgc cgccagcgat
    20041 cgcattgatg cgcaggtcgc gcgctacaaa gaacaattta cccaactgga cgttttaatg
    20101 acctcgttaa acagcaccag cagctactta acgcagcagt tcgaaaacaa cagtaattcc
    20161 aagtaatcat tattcatcgg gagacaggtc atgtacgcgg caaaaggcac ccaggcctat
    20221 gcgcaaattg gcgtcgaaag caccgtaatg agcgccagcc aacagcagtt ggtcaccatg
    20281 ctatttgatg gagtgctgag cgcactggtt agagcgcgac tgtttatgca ggacaacaat
    20341 cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa cggactgcgg
    20401 gtgagtcttg atgaagagag caaagacgaa ttaacccaaa acttaattgc tctttatagc
    20461 tatatggtca ggcgtttgct gcaagccaat ttacgcaacg atgtctccgc agtcgaagag
    20521 gtggaagcat taatgcgtaa tattgccgat gcctggaaag agtctttact ctccccttct
    20581 tcgattcagg acccagtctg atgaacaatg caccgcattt gtatttcgcc tggcaacaac
    20641 tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg gacgaactca
    20701 tcgctagcga aatggcgtat gtgaatgcgg tgcgggagat cgcacacctg actgaagagg
    20761 ttgccccgtc cactacgatg caggagcagc tccgcccgat gctgcacctg attctcgaca
    20821 acgaaagcaa ggtaaagcag ttattacaga ttcggatgga tgaactggcg aaactggtcg
    20881 gtcagtcatc ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc ggctttgtgc
    20941 tggctccgca ggataacctc ttttgaatct taatgagccg atggctcgcg aataatccga
    21001 ttacggctac gcttctaata ttccccttga atggagtcga agaatgcgta atcccacgct
    21061 gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg aactggccga
    21121 gcgcgcagac ggttttaatg atattggtat caatatggtc tggttgccgc ccgcctataa
    21181 aggcgcatcg ggcggctatt cggtcggcta cgactcctat gatttatttg atctaggtga
    21241 gtttgatcag aaaggcagca tccctactaa atatggtgat aaagcacaac tgctggccgc
    21301 cattgacgcc ctgaaacgta atgacattgc ggtgctgctg gacgtggtag tcaaccacaa
    21361 aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg atgaccgtac
    21421 gcaaattgac gaagaaatca ttgagtgtga aggctggacg cgttacacct tccccgctcg
    21481 tgccgggcaa tactcgcagt ttatctggga tttcaaatgt tttagcggta tcgaccatat
    21541 cgaacaccct gatgaagatt gcatttttaa aattgttaac gactataccg gagaaggctg
    21601 gaacgatcag gttgatgatg aactcggtaa tttcgattat ctgatgggtg agaatatcga
    21661 ttttcgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg tgatggaaca
    21721 aacgcaatgc gacggctttc gtcttgatgc ggtcaaacat attccggcat ggttttataa
    21781 agagtggatc gaacacgtac aggaagttgc gccaaagccg ctgtttattg tggctgaata
    21841 ctggtcgcat gaagtcgata aactgcaaac gtatattgat caggtggaag gcaaaaccat
    21901 gctgtttgat gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg gtcgcgacta
    21961 cgacatgacg cagatcttca ctgggacgct ggtggaagcc gatccttttc acgccgtaac
    22021 cctcgttgcc aaccacgaca cccaaccgtt gcaagccctc gaagcaccgg tcgaaccgtg
    22081 gtttaaaccg ctggcgtatg ccttaatttt gttgcgggaa aatggcgttc cttcggtgtt
    22141 ctatccggac ctctacggtg cacattacga agatgtcggt ggtgacgggc aaacctatcc
    22201 gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc agcgtttcgc
    22261 ccacggtgta cagacattat ttttcgacca tccgaactgc attgccttta gccgcagtgg
    22321 caccgacgaa tatcccggct gcgtggtggt catgtcgaac ggagatgatg gcgaaaaaac
    22381 cattcatctg ggagagaatt acggcaataa aacctggcgt gattttctgg ggaaccggca
    22441 agagagcgta gtgacagacg aaaacggcga agcgaccttc ttttgcaacg gcggcagcgt
    22501 cagcgtgtgg gttatcgaag aggtgattta aatccatccc cggcggcaag ccggggagat
    22561 ttcattacgg cagtggcgtc ggtaacggcg ctttatccag cgcggcggca cactctgccg
    22621 tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta ccgtcacgct
    22681 caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatcagac atcaatgtca
    22741 acttacccgg cacggcaatc ccccgctgcc actgacggca atccaaggta tcgccctctt
    22801 tagtgacaat caggctgcct atcgcttccg gactgaccag cgcacgttgc ggccctttgg
    22861 tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg ttatagtttt
    22921 cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt tttttcatca
    22981 ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gctctgatgt cgccagcctt
    23041 tccaggttca gagagaaatt tgatctaact attatttttg tgaattatta ccccgaaaat
    23101 aaatttgcat ggtgtatgat tcgcggctct tgtttcaaac cttctgagtt cagaggctac
    23161 attcatgtca tggcagcaat tcaaacacgc ctggttgatt aaattctggg cacccatccc
    23221 tgcggtcatc gcggcgggta ttctctccac ttactatttt ggcattactg gcaccttttg
    23281 ggctgtcacg ggtgaattta cccgttgggg cggtcagctc ctgcagctgt ttggcgtcca
    23341 tgctgaagag tggggttatt ttaaaattat ccatctggaa ggatcgccat taacccgcat
    23401 cgacggcatg atgatcctcg gtatgtttgg cggctgcttt gctgctgcgc tgtgggccaa
    23461 caatgtcaaa ctacgcatgc cacgcagccg tatccgcatt atgcaggcca tcattggcgg
    23521 cattatcgcc ggttttggcg cgcgtctggc aatgggctgt aacctggcgg cgttctttac
    23581 cggcattcct cagttctcgc tgcatgcctg gttctttgcc atcgccactg ccattggttc
    23641 atggtttggc gcgcgcttta ccctgctgcc catcttccgt attcccgtga aaatgcagaa
    23701 agtttctgcc gcctcaccgc tgacgcaaaa accggatcag gcgcggcgtc gttttcgtct
    23761 cgggatgctg gtctttttcg gcatgctggg ctgggcgctg ctcacggcga tgaaccaacc
    23821 aaaactgggg ctggcaatgc tgtttggcgt cggctttggt ttactgattg aacgtgcgca
    23881 aatctgcttt acttcggcgt tccgcgatat gtggatcacc ggacgtaccc atatggcgaa
    23941 agcaatcatt atcggtatgg cggtgagtgc catcgggatc ttcagttacg tacagttagg
    24001 cgttgaacct aaaatcatgt gggcgggacc aaatgcggta attggtggtt tactttttgg
    24061 ttttggcatc gtgctggcag gcggctgcga aaccggctgg atgtaccgcg cggtagaagg
    24121 ccaggtgcac tactggtggg tcggtctggg caacgtgatc ggctcaacga ttctggcgta
    24181 ttactgggat gatttcgctc cggcgctggc caccgactgg gacaaaatca acctgctgaa
    24241 aacttttggt ccgatgggag gcctgctggt gacatatttg ctgctgtttg ctgcgctgat
    24301 gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg gcaccgcaga ctgctaagga
    24361 gatcgcatga aaaatatcgt tcctgattac cgccttgata tggtgggtga accctgccct
    24421 tatccggcag tcgcaaccct tgaggcgatg ccgcaattaa aaaaaggtga gatcctagaa
    24481 gtggtgagcg actgtccgca gtcgatcaat aatattccac tggatgcgcg taatcacggc
    24541 tacactgtgc tggatattca gcaagacggg ccgaccattc gttatttaat tcagaagtaa
    24601 tccctcattc ccgctgtggc ccacagcggg aacatcgtta cctctgacac atagatttta
    24661 cccgccccct ctcctattct tttcagtatg atctcaggag gttgctatgt gtggacgctg
    24721 tgcccaatcc caaacgcgtg aggattacct tgcgcttctc gcggaagata ttgaacgcga
    24781 tattccctac gatcccgaac ccattggcag atacaatgtc gcgccgggaa ccaaagttct
    24841 tctgctcagt gaacgtgatg aacaccttca tctggatccg gttttctggg gatatgctcc
    24901 cggatggtgg gataaaccgc cgctgattaa cgcccgcgta gaaactgcct caaccagtcg
    24961 catgtttaaa ccgctctggc aacatggtcg ggcaatctgt tttgccgatg gctggtttga
    25021 gtggaaaaaa gaaggcgaca aaaaaagcct tattttatct atcgcgctga cggacaacct
    25081 gtttttatgg ccgcgatagg tagcataccg tttgagcgtg gtgacgaagc cgaaggattt
    25141 ttgatcgtca ctgcggcggc agatcaaggt ctggtagata ttcatgaccg ccgcccgctg
    25201 gttctgtcgc cagaagctgc acgggaatgg atgcggcaag acattggtgg taaagaagcc
    25261 tcagaaatcg ctaccagaag ctgtgtgcca gcaaaccagt tcacctggca ccctgtgtcg
    25321 cgcacggtgg gtaatgtcaa aaaccagggc gcggagttaa ttcaacctgt ttgttgacat
    25381 cctccacgcc ctgaatgacg aggccccctg ctacgttcag gctgtcgcct gaatcatttc
    25441 ggtgggttcc tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc
    25501 gtgccccgcc gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca
    25561 tacctggcat ctgaatttac tttgtgacag gcgattttct ttcgctgtat gaccacagc
//
LOCUS       DAJTNM010000046        25582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTNM010000046 DAJTNM010000000
VERSION     DAJTNM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.046"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            336..629
                     /locus_tag="OO172_002036"
     CDS             336..629
                     /locus_tag="OO172_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5512231.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            701..1360
                     /locus_tag="OO172_002037"
     CDS             701..1360
                     /locus_tag="OO172_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5512232.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1452..1898
                     /locus_tag="OO172_002038"
     CDS             1452..1898
                     /locus_tag="OO172_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5512233.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2105..3005)
                     /gene="hlyE"
                     /locus_tag="OO172_002039"
                     /pseudo
     CDS             complement(2105..3005)
                     /gene="hlyE"
                     /locus_tag="OO172_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3378..3797
                     /locus_tag="OO172_002040"
     CDS             3378..3797
                     /locus_tag="OO172_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5512234.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQRL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3797..5063
                     /gene="umuC"
                     /locus_tag="OO172_002041"
                     /pseudo
     CDS             3797..5063
                     /gene="umuC"
                     /locus_tag="OO172_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(5109..5639)
                     /gene="dsbB"
                     /locus_tag="OO172_002042"
     CDS             complement(5109..5639)
                     /gene="dsbB"
                     /locus_tag="OO172_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5512235.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5785..7326)
                     /gene="nhaB"
                     /locus_tag="OO172_002043"
     CDS             complement(5785..7326)
                     /gene="nhaB"
                     /locus_tag="OO172_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5512236.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7547..8266
                     /gene="fadR"
                     /locus_tag="OO172_002044"
     CDS             7547..8266
                     /gene="fadR"
                     /locus_tag="OO172_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5512237.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8318..9850)
                     /locus_tag="OO172_002045"
     CDS             complement(8318..9850)
                     /locus_tag="OO172_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5512238.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHTCYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSHEEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10180..11478
                     /gene="dadA"
                     /locus_tag="OO172_002046"
     CDS             10180..11478
                     /gene="dadA"
                     /locus_tag="OO172_002046"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5512239.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11488..12558
                     /gene="dadX"
                     /locus_tag="OO172_002047"
     CDS             11488..12558
                     /gene="dadX"
                     /locus_tag="OO172_002047"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5512240.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12726..14152)
                     /locus_tag="OO172_002048"
                     /pseudo
     CDS             complement(12726..14152)
                     /locus_tag="OO172_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14762..16498)
                     /locus_tag="OO172_002049"
     CDS             complement(14762..16498)
                     /locus_tag="OO172_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5512241.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16593..17507)
                     /gene="ldcA"
                     /locus_tag="OO172_002050"
     CDS             complement(16593..17507)
                     /gene="ldcA"
                     /locus_tag="OO172_002050"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5512242.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17607..18218
                     /gene="emtA"
                     /locus_tag="OO172_002051"
     CDS             17607..18218
                     /gene="emtA"
                     /locus_tag="OO172_002051"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5512243.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18220..18954)
                     /locus_tag="OO172_002052"
     CDS             complement(18220..18954)
                     /locus_tag="OO172_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5512244.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19155..19409
                     /locus_tag="OO172_002053"
     CDS             19155..19409
                     /locus_tag="OO172_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5512245.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19459..20919)
                     /locus_tag="OO172_002054"
     CDS             complement(19459..20919)
                     /locus_tag="OO172_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5512246.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20856..21413)
                     /locus_tag="OO172_002055"
     CDS             complement(20856..21413)
                     /locus_tag="OO172_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5512247.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARVLTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21439..22293)
                     /locus_tag="OO172_002056"
     CDS             complement(21439..22293)
                     /locus_tag="OO172_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5512248.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22293..23102)
                     /locus_tag="OO172_002057"
     CDS             complement(22293..23102)
                     /locus_tag="OO172_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5512249.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23112..23870)
                     /locus_tag="OO172_002058"
     CDS             complement(23112..23870)
                     /locus_tag="OO172_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5512250.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23867..24847)
                     /locus_tag="OO172_002059"
     CDS             complement(23867..24847)
                     /locus_tag="OO172_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5512251.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISP
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24847..>25582)
                     /locus_tag="OO172_002060"
     CDS             complement(24847..>25582)
                     /locus_tag="OO172_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5512252.1"
                     /translation="ASTLSLEGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLL
                     LNGKLAQSAEQLTTLGKTLGEEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGP
                     RGLETGLQGSLHTEAAELLGLHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTAD
                     FIRRDPLWQGVKAVAEQRILFLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFK
                     SDMQHYAQLFWHCSLSDADYQKLVAS"
BASE COUNT         6371 a         6304 c         6630 g         6277 t
ORIGIN      
        1 tgaactcaac attgacattc ttacccgcag caaagctcgt ggaggttagc agtgcaagca
       61 gcaatacagc cttactgatg ctcttgattt tcattggtag tctcctgttc agaatgagta
      121 cgatgaactg ttataatata acaatccctt acgggagaat tccagtggct tttctgcgtt
      181 cggtaaaatc ccggcgcggc attttttgcc ggggggatta cttataaagg ggcatggact
      241 caacaaaccg cagaaatcta cagctggctg gcttgtggcg cgggtttcat tgatgttagc
      301 ttatgccaaa acctggcatc ttaaagagta aaagtatgtt ttgtgtgatt tatcgaagca
      361 gcaagcgtga ccagacctat ttatatgtcg aaaaaaaaga cgatttttcg cgtgttcctg
      421 aggaactgat gaaaggtttt ggtcagcctc agttagcgat gattctgccg ttggatgggc
      481 ggaagaaact ggtcaatgct gatattgaaa aggttaaact ggcattaacc gagcaaggtt
      541 actatttgca gttaccgcca ccacccgaag atttgctgaa gcaacatctt tccgtcatgg
      601 ggcagaaaac agacgacact aacaaataac cgatatccgg cggtggcatt atctttgtcg
      661 gcgcgggttt tcatatccac gataaggtga ggggaacgtt atgtatcaac atcacaactg
      721 gcaaggtgcg ctgctggatt atccggtgag taaagtagtc tgtgttggca gtaactatgc
      781 caaacatatt aaagagatgg gcagcgcagt gcccgaagag ccagtgctgt ttattaaacc
      841 agaaacggca ctgtgcgatc tgcggcagcc attggcgatc ccatccgatt tcggttcagt
      901 tcatcatgaa gtcgaactgg cggtgttgat tggcgcgacg ctgcgtcagg caacggaaga
      961 gcatgtccgc aaagccattg ccggttacgg cgtggcgctt gatctgacat tgcgtgatgt
     1021 tcagggaaaa atgaagaaag ccgggcagcc gtgggaaaag gctaaagcgt ttgataactc
     1081 ttgtccgctt tccgggttta tccccgcggc ggaatttacc ggcgatccac aaaatacaac
     1141 gctgggcctg agcgtaaacg gcgaaaaacg ccagcaaggt acgactgcgg acatgatcca
     1201 taaaatcgtt ccgttgatcg cttatatgag caagtttttt accctcaagg ccggtgacgt
     1261 tgtgttgaca ggcacgcctg atggcgtcgg cccgttgcaa agcggtgatg agctgacagt
     1321 cactttcgat gggcattctt tgacaactcg cgttttgtaa tactttttgc cgccagaaag
     1381 cggcggcaaa acttgcatcg ctgtgccaga ctggttataa ggtgcgtttt aacgtaatgg
     1441 cggaacacct gatgagcgat gtacctttct ggcaaagtaa aaccctggac gaaatgagcg
     1501 atgcggaatg ggagtcgttg tgtgatggtt gcggtcagtg ttgcctgcat aaactgatgg
     1561 atgaagacac cgacgaaatc tacttcacta acgtcgcctg tcgccagctc aatattaaaa
     1621 cctgtcaatg tcggaactac gaacgtcgtt tcgagtttga acccgactgc attaaattaa
     1681 cccgtgaaaa tctgccaaca ttcgaatggc tgccaatgac ctgcgcttat cggttgctgg
     1741 cggaaggtaa agatttacct gcgtggcatc cgctacttac tggttcgaaa gcggcaatgc
     1801 atggtgaacg tatctctgtg cgtcatatcg cagtgaaaga atcagaagtg attgactggc
     1861 aggatcatat cttaaataaa cctgactggg cgcagtgata aatttaatag atgctgatgg
     1921 gggctttctt aatatacgtc tggatgctcg ccatttcgta gccctgtttt tgaaagcgct
     1981 ctgtcataat gagagttacc cgatacctta ttatcaacag gtaactctca atctttaaaa
     2041 aacgtctgtg tttttaaatg tgataaacct tataccttga gtacatggag agaataatcg
     2101 cttatcagac ttcaggtact tcaaagagtg tctttttacc gtgtcttttc tgatactcat
     2161 tacaggtgtt aatcattttt ttggccgctt cttttagcaa agaaagcatt aaatcatcat
     2221 aatcaacata gaatctggtt gtttcagttt ccgtttttat ctccccgatg gcggctattt
     2281 cggtggttaa tttcaatttg gcggcatcga tatctttatt cgcttgttta accgtgttag
     2341 acagggtggt aaagaaactc tgcacagatt ttaacttgtt cttcaattct ggaatcagtt
     2401 tcccttcaac tacgcccgca gcaatagaat aggaaatgat taaaccaaat ggaccggcga
     2461 cgacatcggc tgcggcaccg gcatacgctt ccttcctgat tttatctacc tgtgactgga
     2521 aatagctgct tttttctgaa aaatcattgg ttaactggct atctaacgcc agcagtttcc
     2581 cggaagcgtt gttgaaactt tgtgagctta ccagcaggga tttttgcgct tcattcagct
     2641 tcgtgatgcc gtcatccagt accttaatga ggggcggatg ctttcttctc attgtactca
     2701 tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa caccacacca ttcatacact
     2761 gtttgggttg cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg
     2821 actaaaacgg aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt
     2881 tcatcaaagg tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct
     2941 ccatctgcgg tttcgattgc gtttttaact acttctaccg ttttatctgc aacgatttca
     3001 gtcataatca ttcgcctctt taaatatata aattgtaatg aaactcctgt tttacaacta
     3061 ttaataaatt ttacttcatc taattcatag ttagccgggc gggatgcgtc aatgtcttta
     3121 tttctattaa tatgataaat atcaaacaat gtttaatgtc attatggcga atgcttctat
     3181 tctatttttt tgccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg
     3241 ccacgtgagc acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga
     3301 tagcctgaat cagtattgat ctgctggcaa gaacagacta ctgtatataa aaacagtata
     3361 gcttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt
     3421 ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggcagca gattacgttg
     3481 aacagcgcat cgatctgaat caacggttga tccagcatcc cagcgcgact tacttcgtca
     3541 aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta ctgattgtcg
     3601 atagcgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac ggcgagttta
     3661 cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac agtgcgtact
     3721 cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg
     3781 ttaaggcgat gcgctgatgt ttgccctctg tgatgtaaac gcgttttatg ccagctgtga
     3841 gacggtgttt cgccctgatt tatggggtac cggtggttgt gctatcgaat aatgacggtt
     3901 gcgttatcgc ccgaaacgct gaggcaaagg cgctgggcgt taaaatgggc gatccctggt
     3961 tcaaacaaaa agatctgttt cgtcgctgtg gtgtggtttg ctttagcagc aattatgagc
     4021 tttacgcaga catgagcaat cgggtgatgt cgacgctgga agaactatcg ccccgcgtcg
     4081 agatttacag tattgatgaa gcattctgcg atctgacagg tgtgcgtaat tgtcgcgatc
     4141 tgactgattt tggcagagaa attcgcgcaa cggtgctaca acgtacccat cttactgttg
     4201 gtgtggggat cgcccagacc aaaacgttgg ctaagcttgc caatcatgcg gcaaaaaaat
     4261 ggcagcggca gacgggtggg gtggtggatt tatcaaatct ggaacgccag cgtaaattaa
     4321 tgtctgctct ccccgtggat gacgtctggg ggattggacg gcggatcagc aaaaaactgg
     4381 acgcgatggg gatcaaaacc gttctcgatt tggcggatac agatatccgg tttatccgta
     4441 aacattttaa tgtcatgctc gaaagaacgg tgcgtgaact gcgcggcgaa ccctgtttgc
     4501 aactggaaga gtttgcaccg acgaagcagg aaattatctg ttcccgctcg tttggtgaac
     4561 gcatcacgga ttatacgtcg atgcggcagg ccatttgtag ttacgctgcc cgggcggcgg
     4621 aaaaacttcg cagtgagcat caatattgtc ggtttatctc cacatttatt aagacgtcac
     4681 catttgcgct caatgaacct tattacggca atagcacgtc ggtaaaactg ctgacgccca
     4741 ctcaggacag cagggatatc attaacgctg ctacgcgatc tctggatgcc atctggcaag
     4801 cgggccatcg ctaccaaaaa gcgggcgtga tgctggggga tttcttcagt cagggagtcg
     4861 cgcagctcaa tttattcgat gacaatgcac cgcgccccgg gagtgagcaa ttgatggcgg
     4921 taatggatac tctgaatgct aaagagggca gaggaacact ctattttgcc gggcagggga
     4981 tccaacagca atggcagatg aagcgagcta tgctttcacc acgttataca acgcgaagtt
     5041 ctgatttact gcgggtcaaa taaatatagc ggcaggaaaa aagcgttccc gcaggagcgc
     5101 cgaatggatt agcgaccgaa cagatcacgt tttttcgctt taaacggctg ggaaatcacc
     5161 accagcaccg cgacaatcag gtaagcgata aaaataccga gcagccactg cggcatttcc
     5221 atacctaaaa aatcccactg acgctcggcg caatcgccag aggcgacaaa cacttgcggc
     5281 acccatttat ccagcggcag ccattccggg aaacgaacca taaaatcaca ggtggcaaat
     5341 ggcgaaggat agagctgaag catggtgtgc tcgtaagtta actgcacacc gcggaacgca
     5401 ctatacaacc agataaccat cgctacataa cgcagcggag ttttcggggc gatcgcgcca
     5461 atcagcgcag cacccagaac gccgaataac gcgcagcgtt cataaataca gagcacgcaa
     5521 ggtttcagta acatcacatg ctggaaccac agcgccgtca gttccagtgc cagagcagta
     5581 aacgccatca acagccacgc gccccggcct tgtgaacatt ggttcaaaaa tcgcaacata
     5641 atcatttccc tgcaatatgc atagagtgcg cagtttaaac caattcattc gctgcgccac
     5701 caggaggcag caaaaatagg acgcaattgg acaattatcg ggcgaaaagg caaaccgggc
     5761 atttatatgc ccggtaagtt gttatcaaag cgttgctatc cagcccattt gcataaacca
     5821 ttcggttaca ggggcaagcg taaactcgac gcagagcaag ccgacgagtg tcagtacgag
     5881 ggtgtaaggc agggccatcc acaccatgcg gccataagag aggcgaatca atggcgcgag
     5941 tgcagaggtc agcaggaaca ggaacgcagc ctgaccgttc ggcgtagcga cagagggcag
     6001 attggtaccg gtattaatgg cgaccgccag cagctcgtat tgcttcaacg tgatagcgcc
     6061 actttccatt gccgctttcg cttcgttgat ataaatcgtc cccacgaaga cgttatccga
     6121 aatggatgac agcagaccgt tgaaaatata gaacagcgac agctgagcat gttcagatgc
     6181 ctgcaacaca aactgaataa tcggcgaaaa cagttgttgg tcgataatca ccgcgactac
     6241 cgagaaaaac accgtcaaca gtgcggtgaa tggcagagat tcggtgaagg ctttaccgat
     6301 agcatgttca tcggtgacac cggtcaatga tgttgccaga ataatgactg acaaaccaat
     6361 caagccaact tccgccagat gcaacgccag cgcagtcacc agccagacgc caataatcgc
     6421 ctggacaatc agacggattt tatcctgacg ggtacgctgg tggcggcttt gatcgtcaaa
     6481 ctgttgcaac acttcgcgga ctttctccgg cagcgtttca ccgtagccaa accaacgcag
     6541 cttctctacc agcaggcagg ttaacaggcc acaaatcaga accggaacgg tcaccggcga
     6601 catgcgcagg aagaaatcgc caaaatgcca gccagccgct ttagcgatga tcaggttctg
     6661 tggttcgccc accatggtca ttacgccgcc taatgcggtg ccgacaccgg catgcatcat
     6721 caggctacgc agaaagccac ggaactgttc cagaaccacg ttgtaatgct tgtcgatatg
     6781 actatcgtct tgcaggtcgg tgtcttcggt acgggaagag gctacgcgat gataaatacc
     6841 ataaaaaccg actgcaacgc tgatcaccac cgccacgacg gttaaggcat cgaggaacgc
     6901 ggagaggaac gctgccgcca cgcaaaaaga gagcgacagc agcattttgg agcgaatgct
     6961 taacagcaaa cgggtaaata tgaacagcaa cagctgtttc ataaaataga tacccgccac
     7021 cataaacatc aataacagca agacttcaag atttgccgcc accccttcac ggacgtgttc
     7081 cgcgctggtc atgccgatga ataccgcttc gatagccaac agaccaccgg ggagtagcgg
     7141 gtagcatttc agggccatcg ccagagtgaa aataaattcc gctaccagca accagcccgc
     7201 gacgaaaggg ctgatgagga aaattaacgg gtttacgatt aagaaaatga tgagggcgag
     7261 tttgtaccag tcgggggact ggcccaaaaa attgcgccat agcgcgcggc cccaggagat
     7321 ctccatgatg gtttccctta ccttacaaat aatcaatgat gtttttgtgt ttaaacgcaa
     7381 agcttaacgg tcaggcagga gtgaggcaag tcttgatagt caaggggaaa gagatgcgga
     7441 aaatgaagcc ttgatccctt tttcttcttt ttgtctgcta tcagcgtagt tagccctctg
     7501 gtatgatgag tccaactttg ttttgctgtg ttatggaaat ctcactatgg tcattaaggc
     7561 gcaaagcccg gcgggtttcg cggaagagta cattattgaa agtatctgga ataaccgctt
     7621 ccctcccggg actattttgc ccgcagaacg tgaactttca gaattaattg gcgtaacgcg
     7681 tactacgtta cgtgaagtgt tacagcgtct ggcacgagat ggctggttga ccattcaaca
     7741 tggcaagccg acgaaggtga ataatttctg ggaaacttcc ggtttaaata tccttgaaac
     7801 actggcgcga ctggatcacg aaagtgtgcc gcagcttatt gataatttgc tgtcggtgcg
     7861 taccaatatt tccactattt ttattcgcac cgcgtttcgt cagcatcccg ataaagcgca
     7921 ggaagtgctg gctaccgcta atgaagtggc cgatcacgcc gatgcctttg ccgagctgga
     7981 ttacaacata ttccgcggcc tggcgtttgc ttccggcaac ccgatttacg gtctgatcct
     8041 taacgggatg aaagggctgt atacgcgcat tggtcgtcac tatttcgcca atccggaagc
     8101 gcgcagtctg gcgctgggct tctaccacaa actgtcggcg ttgtgcagtg aaggcgcgca
     8161 cgatcaggtg tacgaaacag tgcgtcgcta tgggcatgag agtggcgaga tttggcaccg
     8221 gatgcagaaa aatctgccgg gtgatttagc cattcagggg cgataatccc ttccgtttaa
     8281 agagcaaacc cctcaaacga ggggtttttt gttgttttta cagatttccc attcttggcg
     8341 ggcaacgttc cagcaactcg atgctgccgt cttcgttttg ctgttcgagc atcacatcaa
     8401 atccccacag gcgatgcaca tgcttcagga cttctttgcg cccccgatcc agcggtgcgc
     8461 gattatgtgg aatataacgc agcgtcagcg aacggtcgcc gcgcaaatcc acgttccaga
     8521 tctgaatatt cggctccaga ttacttaagt tatattgcga cgataaccgg ttacggatct
     8581 cccgataacc ttcttcatta tgaatagcgg aaatctccag ataattatgc cgatcatcat
     8641 ccagcacggt gaagaagcgg aaatcacgca tcactttcgg tgacagaaac tggctgataa
     8701 aactctcatc tttgaaatca cgcatcgcga aatgcagcgt ttccagccag tcggaaccgg
     8761 cgatatccgg gaaccagtat ttgtcttctt ccgttggcga ctgacaaatc cgtttaatat
     8821 cctggaacat ggcgaacccg agggcatacg ggttgatgcc gctgtaccac gggctgttat
     8881 aggggggctg gaagaccaca ttggtgtggc tgtgcaaaaa ctccagcata aaacgttccg
     8941 ttactttccc ttcatcatac agatggttaa ggatggtata gtgccagaag gtcgcacagc
     9001 cttcgttcat aacctgagtc tgtttttgcg gataaaaata ctggctcacc ttacgcacaa
     9061 tacgcaggat ttcacgctgc catgattcca gcagtggcgc atttttctcc ataaaataga
     9121 gcaggttttc ttgtggttcg gacggatagc ggcgcgcttc agcaaccgtt ttctcttcct
     9181 cgcgcttcgg cagggtacgc cagagcatat tgacctgact ttgtagatac tcttcgtgac
     9241 ttttctgccg ggctttctct tcttgcagcg agattttttg cgggcgtttg taccggtcca
     9301 cgccgtagtt catcagcgcg tggcacgagt ccagaagccg ttctacttca tcaacgccat
     9361 aacgttcttc gcactcggta atatatttac gggcaaaaat cagataatcg acaatcgaac
     9421 tggcgtcggt ccaactgcgg aataagcaat tgtttttaaa gaaagagtta tgcccatagc
     9481 aggtgtgtgc catcaccagc gcctgcatag taatggtgtt ctcttccatc aagtaagcga
     9541 tacacgggtt agagttaatg acgatttcat aggccagccc ttgctgaccg tgcttataca
     9601 gccgttcagt ctcgataaac tttttaccga atgaccagtg cggatagtta attggcatgc
     9661 cgacgctgga ataggcatcc atcatctgtt ctgaggttat cacttcaatc tggtgcgggt
     9721 aagtatccag ccggtagagt ttcgccaccc ggtctatctc tgccagataa acatccagca
     9781 ggtcgaacgt ccagtcgggt ccatcgctca aacgtgtggt gtccttatta atagaatcga
     9841 tcgtcgccat acgcgcacct cattgttgtc ggcgctctct gtgtggagca cctcatttca
     9901 agcatagaac acctgttaaa aaccgcgtcg ccggagaatt tttttctttg cgatttctta
     9961 ttatcagagt gccactaatc cgcttctgaa cggaatttta tgctggataa aaagggcgtt
    10021 cagcaggaga tactaaagac gccatattgc cgcagagtca gggagatgtg agtcagctca
    10081 ccataaaaaa gccgtatgtt gaataatatt ttcaactgag ttatcaagat gtaattagat
    10141 tattattctt ttactgtatc taccgttatc ggagtggcta tgcgagttgt catactggga
    10201 agtggtgtgg taggcgttgc cagcgcctgg tacttaaatc aggcaggaca tgaggtcacc
    10261 gtcattgatc gggagccggg ggccgccctg gaaaccagtg ccgcgaatgc cgggcaaatc
    10321 tcccccggat atgctgcacc gtgggcagca ccaggtgtgc ctttaaaagc gattaaatgg
    10381 atgttccagc gccatgcgcc gctggcggtt cgtctcgacg gtacgcagtt tcagttgaaa
    10441 tggatgtggc aaatgttacg taactgcgac accagccact acatggaaaa caaagggcgg
    10501 atggtgcgtc tggcggaata cagccgtgat tgcctgaaag cattgcgcgc cgaaaccaat
    10561 attcagtatg aagggcgtca gggtgggacg ctgcaactgt tccgtaccga acaacagtat
    10621 gaaaatgcga cccgcgatat cgccgtgctg gaagatgccg gcgtaccgta tcagctgctg
    10681 gaatccagcc gcctggcgga agtggatccc gcgctggcag aagtagcgca caaactgacg
    10741 ggcggcctgc agttacccaa tgatgaaacc ggagactgcc agctgtttac ccagaatctg
    10801 gcgcggatgg cagagcaggc gggggttaaa ttccgcttta atacgcccgt tgaccaactg
    10861 ctttgcgacg gcgagcaaat ctacggcgtg aagtttggcg atgaagtgat taaggccgat
    10921 gcgtatgtga tggcgtttgg ttcttactcg acggcgatgc tcaaaggcat tgttgatatt
    10981 ccggtgtatc cgctgaaagg ctactcgctg actattccaa ttgcgcagga agatggtgcg
    11041 ccagtatcca ccattcttga tgaaacctac aaaatcgcca ttacccgttt cgataaccgc
    11101 attcgtgttg gcggaatggc ggaaattgtt ggttttaaca ccgagctgtt gcaaccgcgt
    11161 cgtgaaacgc tggaaatggt ggttcgcgat ctctatccac gcggcggtca tgtcgagcag
    11221 gcgactttct ggacgggttt gcgcccgatg acgccagacg gtacgccggt tgtcgggcgt
    11281 acacgcttta aaaatctgtg gctgaatacc ggtcacggca cgctcggctg gacgatggct
    11341 tgcggttccg gtcagttgtt aagcgatctg ctctctggtc gcacgccagc gatcccatat
    11401 gaggatctaa gcgtagcgcg ctacagccgt ggatttacgc catcacgtcc gggccattta
    11461 catggcgcac acagctaagg aaacgagatg acccgtccga tacaggccag cctcgatctg
    11521 caggcattaa aacagaatct gtccattgtc cgccaggccg cgccgcacgc gcgcgtctgg
    11581 tcggtggtaa aagcgaacgc ttacgggcat ggtattgagc gtatctggag cgcgctcggg
    11641 gccaccgatg gctttgcatt acttaacctg gaagaggcaa taacgttacg tgagcgcggc
    11701 tggaaggggc cgatcctgat gctggaagga tttttccatg ctcaggatct ggagatttat
    11761 gaccagcacc gcctgaccac ctgcgtacac agcaactggc agctcaaagc actgcaaaat
    11821 gcgcggctaa aagcaccgtt ggatatttat cttaaagtga acagtgggat gaatcggttg
    11881 ggcttccagc ccgatcgcgt gcttaccgtc tggcagcagt tgcgggcaat ggcgaatgtt
    11941 ggcgaaatga ccctgatgtc gcattttgct gaagcggaac atcctgatgg aatttccggc
    12001 gcgatggcgc gtattgagca ggcggcggag gggctggagt gtcggcgttc gttgtccaat
    12061 tcggcggcga ctctgtggca cccggaagcg cattttgact gggttcggcc tggcattatt
    12121 ttgtatggcg cttcgccgtc cggtcagtgg cgtgatatcg ccaataccgg attacgtccg
    12181 gtgatgacgc taagcagtga gattattggt gtccagacgc taaaagcggg cgagcgtgtg
    12241 ggctacggcg gtcgctatac tgcgcgcgat gaacagcgaa tcggcattgt cgccgcagga
    12301 tacgccgacg gttatccgcg ccatgcgcct accggtactc ctgttttagt ggacggcgtg
    12361 cgcaccatga cggtggggac cgtctcgatg gatatgctgg cggttgattt aacgccttgc
    12421 ccgcaggcgg ggattggtac gccggttgag ctgtggggca aggagatcaa aattgatgat
    12481 gtcgccgccg ctgccggaac ggtgggctat gagttgatgt gcgcgctggc gttacgcgtc
    12541 ccggttgtga cagtgtaact tgttgtaagc cggatcggag gcagcgtctt ctggttgcga
    12601 aaaaatcatc catccggctg gtcagcaact gtagttgtta atgtgacaga gccattgccc
    12661 atgatagtgt ccattaaaag aatggacact atttccccgg aacctgaact cgccgcacag
    12721 gcgttctaca taaaacgctt acgcttcatt gttgactcgg agtcgactta gataaatcac
    12781 gcttcaccct tgatgcagat tcgggttcat gttcagattg ttgattaaca ggctgcgagt
    12841 cctgttgtct ttccagactc aacaggccca actgttgatc caggagctcc aggtcctcct
    12901 gtaccttcac gcgctccagg agttccgggt catccaggaa ctccagatcc ataaggtcct
    12961 ccagctcctc gagtccttcc aggtcaacct agccttcctt gtgcaaaaag tccaaaaggt
    13021 ccttcaggcc ctgctgtttt ttcaggccct cgacggcttt gagcagcgtc ggtaaattgt
    13081 ctttgtccca cgggttacca ttacagtcaa acaccttcag gctctggggc tgcggcacct
    13141 gtaccgtttt aaacttgttt tctccacagt agtaaattat tagtttgggc ggcagccgtg
    13201 gtgaagagat caaatcgtta ctagaacagt caaatacttt caggcgagtc aagttctcca
    13261 gcatctcatc aggtattgag ttaatattgt tgccggcaca ggatagttta tacaggtact
    13321 ggggcatcga cggcggtaaa aattgcaaga agttttcaga acagttaatc actctcaggt
    13381 tagtcgacag ctcaggcagc cagataatac agttttcata gcacttcagt accatcagtt
    13441 ttgagggctg caccttcggt aacaagatca agacgttatc tgtacagtca agcaccagca
    13501 gccactttgg catcaccttc ggtaacgcgg tcaacacgtt tttactgcag taaaattcaa
    13561 tcagatgtgg aggcaaattc tcagggagtg aagtcaatcc caggttgcta agattgagca
    13621 tactccgtct tgtaatgaga cattccttca ttctgcgcaa tgcttctgcc cggttttcaa
    13681 tccggcttgc tggcaggttt tcttccagga tattttcctt gatttcttcc agactttctt
    13741 tcaggtcttc ttggggaatt tctgactgtt tgttttgctg gttttcttcc aggatttcgt
    13801 acaggttttt ttgcaggttt tcttgcaaac catcctgctg tatagcccat tcgcaccatt
    13861 tggcgaccat catgaactcg ttatgttgct gattcaggcg agtccccaac gtgatgatgg
    13921 tattgttgtc cacctgtatg ttcactttgc ccgcgatact gaggtcaatg atctgccagg
    13981 gggcatcacc ccagctgaat ttttgcgggc tgagatactt catgttctgc aaaatttgat
    14041 tcttgagttc caccgacagg aagagccgcc ctggcgggcg tcgtacgtct gggttaacgg
    14101 aagatggggg aatggcgggg gcgaatgggc agatgtcttt aaagctagcc ataatgctac
    14161 gtttccttga aataaatatc ctctggcagc gccggagacg tgaatgtggt caccgcaaag
    14221 gagccacagg acgtcatttg cgctgatatc actactcatc gttgactttt tccctggtca
    14281 gaaatgccgt gtcgggtctg gtacatgctg tattacggcc taggtagctc tgacgtatta
    14341 aagaaaatac gagagtgaac gacccggaaa taatcgttat taagcaaagg aataactata
    14401 agaaatcgct catgcatccg gtgctacagg cggatgaaac gcctattgtg tgggctatgt
    14461 cagtgcggca ggttgcgtgg ggaatatgtt caggcgatgt caggcagggg cccctgatgg
    14521 ttgaccggta atgcggggct ggtttttatt acctgttaca gtcggaaaaa gcccttacgc
    14581 tgactttctt gagccggtgt tttgagttca ttagccagag cctgcaatgg tttgtcatcc
    14641 ataatttaac cttcttctga tgttgggatg agcatatcaa aatcaggaaa tgacaagttt
    14701 attacaggct caggctgcgt tcagtaaagc atcgcatccg gcgatagatg ctgcattaaa
    14761 tttaagattc agcttcctct tcagccaccc gaacgccaat cttcaacact tcattatctt
    14821 ctttctcggc caccgtccag atcatcccgg caaactctac ctggtcaccg acaaccggtg
    14881 ccgcgcctag caactgctgg acaatttcac ccagcgtttg ctgtttatca cgatattctc
    14941 gcccgtcttc caggccatat atcagcgcaa catcagcata tttggcgctg gcttcgagaa
    15001 tgaagtcacc aaagaagcgt tgatccagcg cgaccggtgg cgactggctg aacagtttac
    15061 cgagcgccgg gagatcgcgt tcccgaccaa ttacacacaa tacatcgcct tcgcgcagtc
    15121 gggtgctgcc ggtaggatga agcaactggt tatcacgaaa cagggctgca atacgcgtct
    15181 cttttggcat atgcaaatcg cgcagtgccg cacctacaca ccatttatca gcactcaatt
    15241 gataaacaaa ctgctcccac ggattttccg gatgaatatc caggccaacg cgtgacaccg
    15301 gacgtccgac gggcggaacc accactttgg cttttttcgc cgcccacgag agtgatgttc
    15361 cctgcaagag cagtgaaacc agaaccacaa agaaggcgac attaaagaac agtcgcgcat
    15421 tctccagccc cgccatcatc gggaacacgg ccaggatgat tggcaccgcg ccgcgtaatc
    15481 ccacccagct gataaacacg cgctcacgca gattgaaacc gcggaaaggg agcaatccag
    15541 caaataccga aaggggacgg gcgaagaata tcatccatgc cgacaaaatg agcgccggaa
    15601 tggcaatagg cagcagatcg cttgggttaa ccaatagccc cagcaccagg aacatggcga
    15661 tttgcgccag ccaggcgagg ccgtcgaaat tttgcaggat gccgtagcgg ttgcgaatcg
    15721 gccgattacc cagcagaaaa ccgcacagat aaacagccag aataccgctg ccttccagcg
    15781 ccgtggttaa agcaaaaatt aaaataccgc cgcttaatgc cagcaatgga tataatccgg
    15841 caggcagggc gatgcgatta atcatttgca gcagtaaata accaccgcca agaccgatga
    15901 caatcccgag gccaaattgc tgcagaatat cgaccacgaa catccaactg acactgcttt
    15961 catgctgctg gatcattgca atcagggtaa tcgtcagaaa gaccgccatt ggatcattac
    16021 tgccggattc aatttccagc gtcgagccaa cacgttcgtt aagcccctta ccacccagca
    16081 gagaaaagac cgctgcagca tcggtggagc cgacgatagc gccgattaat aagccttcaa
    16141 tcaaatcaag attaaacagc cacgccgcca tcatgccggt taaaccagag gtgataagta
    16201 cgcccagcgt cgccagcgac agcgccggtc ctaacgccac acgaaaggaa ctggcctgag
    16261 tgcgcatccc gccgtcgagc aaaataatcg ccagcgccag gttacttacc atgtaggcga
    16321 aggggtaatt atcaaacggg ataccgccga cgccatcgac tcccgccagc atgccgatcg
    16381 ccagaaaaat aaccagaata ggaatgccaa gacgggaaga aaatgaacta agtaatatac
    16441 tgctggtgac aaggatggat cctaagatga aaaggctaat tattgttgtg gcatccaacg
    16501 gtcggttact cctgattacg ctgtctctta tataaaccct accatattag cggcagagac
    16561 agcgttttac ttagccctga agcgtgattt ttttacattt taagaacagg atgaccgcta
    16621 atcgtcaact gagtgccttc ccgggtgtta ttcagaatgg catgtgcgcc caacggcagc
    16681 gtaaccgtgc gttgttcatg accaaaatcg aggccggtaa tgagtggaat cgacaggcgg
    16741 gaacgcaaaa acgcgtacac tgactccagg ttgtaacctg cgtcataatc attgggcgtg
    16801 ctgccgctaa agctaccgag aataatcgcc ttctgacgtg gcaaaattcc cgcatgatag
    16861 agctgcaaca gcatacgttc gacgcggaaa gggtgctcgt taatatcttc cagcaccaga
    16921 ataccgttct caatttttgg catccacggt gtaccaatca gtgaaatcag catcgcaaga
    16981 ttgcctcccc acaacgtgcc ttcggtctga catgtcgggc cttccccttg ccattcaatg
    17041 gtgaaggttt cattgcgtaa cgccagccag aaatggtgct cggtaaaggc gttcagttca
    17101 tccgcaccaa agtttgctac cagcatgggg ccgctaaagg tgatgacatt gccttgcgcc
    17161 agaagaccgc actgaatggc ggtaaaatcg ctatgtccgc aaatgagtaa cgggtcatgt
    17221 tgttggcgag ccaccagtgc ctgccagtca atatctgcca gtaaacgact ggcaccgtaa
    17281 ccgccgcgta cagccagcac gatggtgttg ggggtagtca gtctggcaag ggaattgaga
    17341 tcttccagac gctccgtttc cgtacccgca aaacgctcac aacgacgggc aataacctcg
    17401 acgttattga cttgatgccc cgcgtcggta aggcgctgga taccacgcag cgcggcgtgc
    17461 tgtttaatgc tgtaacccga tggggcaatt aagtgaaaca gagacatggc aattccttgc
    17521 tgacaacaga aacgaaatgt atatcatgcc gcttaggtgt gccgttgtca cctcaacggc
    17581 gattccaggc tataaggata gaagaagtga aattgagatg gtttgccttt ttgattgtgt
    17641 tattagcggg ttgttcatca aagcatgact atacgaaccc gccgtggaac gcgaaagttc
    17701 cggtgcaacg tgcgatgcag tggatgccaa taagccagaa agccggtgca gcctggggcg
    17761 tcgatccaca attgatcacg gcgattatcg ctatcgaatc gggtggtaat ccgaacgcgg
    17821 tgagtaaatc gaatgccatt ggtttgatgc agttaaaagc ttcaacctcc ggacgtgatg
    17881 tttatcgccg tatgggctgg agtggtgagc cgacgaccag cgagctgaaa aatccagagc
    17941 gtaatatttc aatgggggcg gcttacctga atattctgga aaccggcccg ctggcaggca
    18001 ttgaagatcc gaaggtactg caatatgcgc tggtggtgtc atacgctaac ggggcaggtg
    18061 cgctgctacg gactttctcg tcagatcgga aaaaggcgat cagcaaaatc aacgatttgg
    18121 atgctgacga gttcctcgat cacgtagcgc gaaatcatcc tgcgccgcag gctccgcgct
    18181 atatctacaa acttgagcag gcactggacg cgatgtaaat cagtcgcgca ctttgtccgc
    18241 tttttcccgg gcttctcgct cgagagagaa aataatccgc tgtaattgcc gctccaccgt
    18301 cgggctgacg ttaagaaaac ggaagctcag acggggagtg gtgatggttt cattcttgcc
    18361 atcaatcact ttgcgctcgc tggtggagat taactgggcg tcaaagtgaa aaacacccca
    18421 ttgccccatg ttgacttcaa tttgagcgaa gcgcatacct tcttgtaatt cggcaggctt
    18481 tgctgtttcc agtaatgcgc ccatgccgcc taacgacaaa tcatacaagc ggaaacgtaa
    18541 cgtactgtta tccgccagtt tggtctggca aaaataaggc ggatggagtg gggcggagat
    18601 gcggaaatat cggcgtcgtt gtacaaacca taaggtggga ggcggtacgg taataaatgc
    18661 cggaagctgc aagtattcac tctgctgtag ttgttcaaca gtaaactcga ctttcgcacc
    18721 ctgagtttcg gcggtaatgg taatgtgctg tgcctttagc acggcgatgt tgtcttcggc
    18781 ttgactgccg aaatccagca ccagtttatc cggggttatt gccagtattt tgctgatcag
    18841 ctgcccgcca ttccaactga gacgcaaagg aattgcggct ttgtgcaaat cgcgtaacac
    18901 gcccaggacg gctaacggat tttgtttcag gaactgctca tggtaatgac tcacgcggaa
    18961 aaactcctga ctgacaaact gtctttggtt tatcggtcat agttaacaaa acttaataca
    19021 aatgcgtgaa tattttttac atgttgttct taaatcagcc gacaaacgcc cagcatcgat
    19081 cccgcccttg cctatactta gagcgttgac gtaagcattt cttgcgaccg attcatcgaa
    19141 acgagggcct gaacatggga attattgcct ggattatttt tggcctgata gccggcatta
    19201 tcgccaagct aatcatgccg gggcgtgatg gtggtggatt tttcctgacc tgtattctcg
    19261 ggatagtcgg tgcggtggtc ggcggctggc tggcgaccat gtttggcatt ggcggctcca
    19321 tcagcggttt taatttgcac agcttcctag tggcggtggt gggcgctatc ctggtactgg
    19381 gcgtattccg cctcctgcga agagaataag attttcataa ggcggatagc gatacagatg
    19441 ccgctatccg ctttcacatc agaacgtata ctcgacacct gcccagaaat tgcgaccttc
    19501 ttcaataaac ccttcactgt aggcatattg ggtatcaaac aggttattga ccgacgcatt
    19561 gacgctgaag ccatgaccta aggtgtaatc ggctcgaatg tgggtcaccg caaaaccggc
    19621 ggctttttgt gaaccgtcac tgttgctgta gctggaggaa cgcgcctctt ccgacagcgt
    19681 tacgcttaac ggctcccatg gtttgagtgt catccatgcg gtcattgtct gcgttggaag
    19741 atcggttatc ttgccgatgt ctttgcgttt ggcgtcagcg tggatcagag cgtagctcaa
    19801 tcctacatcc agtatattgc tgattttccc cttaataccg gcatccagac cgctgtaatc
    19861 caccgtgccg ctgttctgat tttgaatggt atcggcattg atattgtgcg agaggatggc
    19921 atcactcacc cggttatagt aaacgctgac ctcaaatccc cagtcgcgcg tgaaggcacc
    19981 attccaggtt aaatccaccc cgcgagcgcg ttccggcttg agctgcgggt taacaatcgc
    20041 tatctggtta tacgcaggtt tggatgtggt atagcgttct ttcagcgtcg gaaagcgtgt
    20101 gcggtcatag tacgaaagcg ccagagtgtc ttcattggca aagtggtatt tccccatcac
    20161 ctgccagtta aaagctgact gattgttgtc gtcataatgg gtgatgctgc catctttctc
    20221 gtgttttttc gcttctacgc tatcgcgcca gtcatagctg attcccgcca cgacatcgac
    20281 attatcagca gcagcccatt gatattcact ggcgagcgac caggtacgat cttcatagcg
    20341 atcgtaagcg gcgtgcggcg cacctttttc ccggtgtacg tcatctttcc agttaacggc
    20401 aaacgacagc agatcgtttt cacgcacatc ggctgccagt tgtaatccgg caccgtcgct
    20461 gtaatcggaa taatggctgt agctgccttt tttatttttc aaatcagcca gcgagttgta
    20521 catcatcagc gtattttcaa aggtgtcgcg atacagccga cttttcaggg taaaacggtc
    20581 gtttagttgg gtcgctccct gataataaaa actttctttg tcatactctg gccactgcca
    20641 gtagcgtgat ttttgaccac aatttccgct gtatggcggg ttatcttttt caccatcctg
    20701 cttaatgtaa gtcagcgtgt attcatcgtt ttcgcgcggt gtaaaaccga gcttcacaat
    20761 gccgcgttta tcatcagccg atgaattaat catcttgccg tgtttgcctg caatatcatt
    20821 atttacacca tgcggcaggc cgagaaaatc ctgctttagc tggctaccgc tgacttgcaa
    20881 atacccaaga tcgctgctgg cggcaaatga agcatgcata tcgtaggcat tgtcctgact
    20941 acggctccat ccctggcgat atcccagact tgcttcttca gggtggtgat attaatggct
    21001 ccgcccatct gattaggccc atgaagcagc gacgaatacc ctttggaaac ttcgactgcc
    21061 cccagattgt tggtcagaac ccgcgccaga tcgaggttgc cgtcataggg aacataaatg
    21121 ggcacaccgt cgaaatagac cggcacctgc cgactatcaa agccacgaac ttttacctgt
    21181 tcttcgttgc ggctgcctga cttttgcagc accacgccgg ggacgacact taatgcctga
    21241 gcgacattct gtttatccaa ggctttcatg gtgggttgat cgagaacggt ggtcgtcgtc
    21301 gatgataccg gactggacca tacggtcagc gtgtcgctct cctgagccgc tacggcctgc
    21361 tgggtaaagg caagtgataa tgctgtgcag agataacgtt ttttgagtct catagaataa
    21421 attccgttta acaaagaatt aaatagaggc tgtgggttgc agactgactt taatgtcagc
    21481 tggtgccgcg taataaggcg cggaggtaat aaaaaggcga atgccgcagt cgaggtaatt
    21541 tttgagtgtc gtcagattaa ttccgccggt cagagccagc gtgcagtgag gagccagcga
    21601 cggcgctatt tgcgcaattt cagttgcctg ttgcggacta aatttgtcga gctgaagtac
    21661 gtctggttgt gcgcgtaacg cggcgatagc ctctttcggc gtgtcggctt caacaacaat
    21721 tttcttctcg ggtgcgtggc gacgtaactg agcgattgcg cctgaccagc cctgattgtc
    21781 atgaagaaaa tggcggtggt tggcaaacaa taatatggtt tccgcacatc cggcacgatg
    21841 aattagtcct ccggcagcca gaattgcctg cgaagccagt aagcgggtgc ccggaattgc
    21901 ttttcgggtg caggcgatat tgccgtcagg ataacgttca cgaagcaatg ccagcatttg
    21961 agcgagataa tcagaaacac cgcaactcca ctccagcaca ttctggactg ccttccatcc
    22021 ctgatgaagt gctgcggcat taccctgcgc acggattagc cgttgacccg cgttcgcttg
    22081 tgaaccgtcg ctgaccgcgt catcaatagt taatcccagc gtagttaaca tcttacacgc
    22141 gacagaaacg ccgctgacgc agccgccctg acggtgaaaa aacgctatat agccaggctg
    22201 gtgtccaata tttaatgctc gcgtggtcag gtcacctccc tggatatctt ccagcagtaa
    22261 cgcatcgatt tgtgcctgag aaaggaaaat catcacagtg cctcctgcgg tacgctatcc
    22321 caccagatga gcgcccagtc gcggctggca ggggcgaggg cacgagcatc ttgttgttga
    22381 taccagtgat ataaccgctc acgctcatcg tcgttaagcg cgccaagact ccagctgacg
    22441 ttctgctcaa aacgttccca ggtgctgtta tcctgttggc aattttgtcc acggataaaa
    22501 tcaacgtggg cataaattcc catctgatac aggacgttga gcgcaaagat atagttgggt
    22561 aattcgatca cttcccgacc agccgcccgc tgaatggctg gagagacgaa cgaggtgctg
    22621 accagatgag ttgtatagac gcgaagccgt gcctgattat tcagtttgct cattgcatgg
    22681 cgcatatcag ccaccagagt ggagcgggaa gccacggcaa tatcgcagcg tggcaggtca
    22741 ctccagtcct cttcccaggc gcgctggatc cagtggacat tgtcagcctt cagtgcggcg
    22801 gcgcggcggg cggcaacgtt cagcatcccc tgactgtaat caacgccata aaccgtagtg
    22861 agtttgtcag ccagcgccag cgatacggtg cctggtccgc agcccatatc gaataaggtc
    22921 tgagcgcctt gcaggtcaat tttagcgatc agttgttgca ggtagctgtc cgttggcgag
    22981 gcgcaatttt ccgccatttt ctccgcccgt tgatcccagt gatcgggcgt tttttctgtg
    23041 cggtgagcca gtttgagttg ctgcagataa aggtctgcaa aatcaatatc atcaatgagc
    23101 atgattgttc cttagcggct aattgcagag aggtgatgat gaatttggtc ggcagaaacc
    23161 cgatacaggg ccgccagttt gttcgttgtt aactgttcgg tcggtaagcc ttgtgttact
    23221 cgtccatctg gctctacctg gataatgctg tcggcaatgg cgttggcatg gagcggatgg
    23281 tgagtcgaca ttaacattgt catgccgtta tttttgagct gcgccagcgt gtccagaagt
    23341 tggatctgat gaccgaaatc gaggctggag gcgggctcat ccagtaacaa caagcgcggt
    23401 tgttggacga gtgcacgggc gattaaagcc agctgacgtt caccgccgct aagcgtattc
    23461 cagcgtcgtg tcgccagatg caaaatgccc agcttttcca gttgctccgt ggctttcagg
    23521 cgttcttgct taccgggaac ggaaaaagcg cctatggtgg gggccaggcc catcaacacc
    23581 atatccagca cactgaaagc aaaaataccg tcgtgcgcct gcggcaccca ggcaatagcc
    23641 tgtgcgcgct ggcgatggct gagtgtctgc acagggaggc tatccagtag gatctcgcct
    23701 tttaataacg gtataacgcc aagaatggag cgcatgagtg tggttttgcc gcaaccattc
    23761 gcgccaagca gacaacaaat tgttccttgc gacagggtga aggacacgtc gcgtagcact
    23821 ggatggcggg agtaacccag agacgcatga cgaacggcaa gcaaagtcac tggcgacctc
    23881 cgcgtaacaa caaaagcagg aaaaacggtg cgccaacaaa agcggtcaga atgccaagtg
    23941 ggatttctgt tgttccaatg ctgcgcgcca gcgtatcggt aagcaggagc agaatagcgc
    24001 cagtacacat tgccataggc aacagctgct gatggttatg accggtcaac aagcgggcaa
    24061 tgtgcggaac gaccagaccg atccaaccaa ttatgcccgc tattgctacc gtgctggcgg
    24121 tgattagtgt ggcgcagaca atcaatccaa agcgcagacg cgtcacgttc agtcccagcg
    24181 aacgggcttc gtcgtcggaa agcgtgagta aattcatgcg ccagcggaga aaaaacaggg
    24241 gcaaggagcc tatgagaatg atcggtgcag catagcagag atcgcgtaag gtcacggtgg
    24301 acagtccccc cagcaaccag aaggtaattg acggtaactg ggtgtaagga tcggccagcg
    24361 ttttaattgg cgatatcccc gctccgcaca acgttcctaa cgcaatgcct accagcacca
    24421 gggttaagat cggatcgtgg cgggtcaccc gacgcgtaat caaccagacg cccgcgacca
    24481 ccatcagctc gccgcaaaat gcgtacagct ggatgtaaac aatgggtaat ccccacagga
    24541 ttgccgcgca ggcaccaagt ccggcaccgg cggagacgcc taaaatatcc ggtgaaacta
    24601 agggattacg caacattccc tgataggtgg ttccggctcc tgccagcgct gcgcccagta
    24661 acagcgcagc aagaatacgc ggcacgcgga tttgccagaa gacaatttgt tcctgcacag
    24721 acacattttc ttgacgaaga attagcgcca gcagttgctg catatcgaga tgataagccc
    24781 cacttacggc ggcaaaagtg atactgatag ctgccagcag caaaatacat ccgtttacga
    24841 ttctcattag ctcgccacca atttttgata gtcggcatca ctgagtgaac aatgccagaa
    24901 cagttgggcg taatgctgca tgtcactttt aaactggcga ttgatggcgg gatccagcca
    24961 ggcgtgaagt ctgcgtaatc ccagaagacg gttgattccc ggcggggcat cgagccagcc
    25021 aaagggcaag ccacttaaaa ataagatgcg ttgttccgca acggctttca cgccctgcca
    25081 gagaggatca cgacgaataa aatctgcagt aacggcctcc tgaaccagaa taatatccgg
    25141 ctgccagcgc aggagatttt ccatggaaac ctgtgtcagg ccgtggcgat cggctatttg
    25201 cgcgacgttg tgcaaaccta ataattccgc cgcctcggta tgcaacgaac cctgtaagcc
    25261 cgtttccagc cctcggggac cgcgcgcagc ataaaagcgg aggttagcgg cgggtgaggt
    25321 ggcgaatgcc tgagcttcac caacgaagtg gctggcgaga ttggcttgtt ctgcggcgcg
    25381 gtgctcttcg cctaacgttt tgccaagcgt tgttaactgt tctgctgatt gcgccagttt
    25441 tccgttaagc aataaccagg ggatttgtgt ctgttcgcta acctgccgtg cctgggagat
    25501 caaggtttca tccgtattgc cgcaatcaac gaccaaatcg ggatgtaacg ccataagccc
    25561 ttccagcgat agtgtgctgg cg
//
LOCUS       DAJTNM010000047        25355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTNM010000047 DAJTNM010000000
VERSION     DAJTNM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.047"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            5..523
                     /locus_tag="OO172_002061"
     CDS             5..523
                     /locus_tag="OO172_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5512253.1"
                     /translation="MVAAITCISQPKKTTLRDKAMVNYAFDYLRSPGSLPLTTAATEL
                     SAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGL
                     GVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYK
                     NAERLLAGLPAH"
     gene            complement(533..1960)
                     /locus_tag="OO172_002062"
     CDS             complement(533..1960)
                     /locus_tag="OO172_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5512254.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1957..2664)
                     /locus_tag="OO172_002063"
     CDS             complement(1957..2664)
                     /locus_tag="OO172_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5512255.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2822..3805
                     /locus_tag="OO172_002064"
     CDS             2822..3805
                     /locus_tag="OO172_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5512256.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3875..4357)
                     /locus_tag="OO172_002065"
     CDS             complement(3875..4357)
                     /locus_tag="OO172_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5512257.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4592..7174
                     /gene="leuS"
                     /locus_tag="OO172_002066"
     CDS             4592..7174
                     /gene="leuS"
                     /locus_tag="OO172_002066"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5512258.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7189..7770
                     /gene="lptE"
                     /locus_tag="OO172_002067"
     CDS             7189..7770
                     /gene="lptE"
                     /locus_tag="OO172_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5512259.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7770..8801
                     /gene="holA"
                     /locus_tag="OO172_002068"
     CDS             7770..8801
                     /gene="holA"
                     /locus_tag="OO172_002068"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5512260.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRTLFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8803..9444
                     /gene="nadD"
                     /locus_tag="OO172_002069"
     CDS             8803..9444
                     /gene="nadD"
                     /locus_tag="OO172_002069"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5512261.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9468..10079
                     /locus_tag="OO172_002070"
     CDS             9468..10079
                     /locus_tag="OO172_002070"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5512262.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10339..10656
                     /gene="rsfS"
                     /locus_tag="OO172_002071"
                     /gene_synonym="rsfA"
     CDS             10339..10656
                     /gene="rsfS"
                     /locus_tag="OO172_002071"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5512263.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10660..11127
                     /gene="rlmH"
                     /locus_tag="OO172_002072"
     CDS             10660..11127
                     /gene="rlmH"
                     /locus_tag="OO172_002072"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5512264.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11158..13059
                     /gene="mrdA"
                     /locus_tag="OO172_002073"
     CDS             11158..13059
                     /gene="mrdA"
                     /locus_tag="OO172_002073"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5512265.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13062..14174
                     /gene="mrdB"
                     /locus_tag="OO172_002074"
                     /gene_synonym="rodA"
     CDS             13062..14174
                     /gene="mrdB"
                     /locus_tag="OO172_002074"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5512266.1"
                     /translation="MTANPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14185..15273
                     /gene="rlpA"
                     /locus_tag="OO172_002075"
     CDS             14185..15273
                     /gene="rlpA"
                     /locus_tag="OO172_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5512267.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15412..16623
                     /gene="dacA"
                     /locus_tag="OO172_002076"
     CDS             15412..16623
                     /gene="dacA"
                     /locus_tag="OO172_002076"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5512268.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16734..16997
                     /locus_tag="OO172_002077"
     CDS             16734..16997
                     /locus_tag="OO172_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5512269.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17098..17739
                     /gene="lipB"
                     /locus_tag="OO172_002078"
     CDS             17098..17739
                     /gene="lipB"
                     /locus_tag="OO172_002078"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5512270.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17998..18951
                     /locus_tag="OO172_002079"
     CDS             17998..18951
                     /locus_tag="OO172_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5512271.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19160..20125
                     /gene="lipA"
                     /locus_tag="OO172_002080"
     CDS             19160..20125
                     /gene="lipA"
                     /locus_tag="OO172_002080"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5512272.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20226..20429)
                     /gene="tatE"
                     /locus_tag="OO172_002081"
     CDS             complement(20226..20429)
                     /gene="tatE"
                     /locus_tag="OO172_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5512273.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20558..21346)
                     /locus_tag="OO172_002082"
     CDS             complement(20558..21346)
                     /locus_tag="OO172_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5512274.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21439..21822
                     /gene="crcB"
                     /locus_tag="OO172_002083"
     CDS             21439..21822
                     /gene="crcB"
                     /locus_tag="OO172_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5512275.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21876..22085)
                     /gene="cspE"
                     /locus_tag="OO172_002084"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21876..22085)
                     /gene="cspE"
                     /locus_tag="OO172_002084"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5512276.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22260..22820)
                     /gene="pagP"
                     /locus_tag="OO172_002085"
     CDS             complement(22260..22820)
                     /gene="pagP"
                     /locus_tag="OO172_002085"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5512277.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23409..24794
                     /gene="dcuC"
                     /locus_tag="OO172_002086"
     CDS             23409..24794
                     /gene="dcuC"
                     /locus_tag="OO172_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5512278.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24835..25176)
                     /locus_tag="OO172_002087"
                     /pseudo
     CDS             complement(24835..25176)
                     /locus_tag="OO172_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         6477 a         6298 c         6241 g         6339 t
ORIGIN      
        1 ttttatagtt gcagcaatta cctgtattag ccaacccaag aaaacgacgt tgcgtgataa
       61 agccatggtg aattatgcct ttgattattt aagatcacca ggcagtctac cgttaaccac
      121 ggcagccacg gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga
      181 attttatctt catggtagcg acggtaaacc actggattat acacaggcga gatactggta
      241 tgagcaatca gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct
      301 gaaaggcctg ggagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc
      361 tgaacaaggg tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg
      421 tattaatgtt aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta
      481 caaaaatgcg gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga
      541 agagatggag tattttccct atcaaaccgg caaagataaa aatgtagaaa ataattttga
      601 tgacgcccag acagcccagc ccgccatcgt caacgggcat tggctgcggt tgcggctccg
      661 gctcgacggt ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta
      721 atgcttccag tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt
      781 cttctgtcgg ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg
      841 gatgttctgt atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct
      901 cgaccagtga acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa
      961 aacgcgtacg ggcgctgtcg ccggggacat aaagtagcgg ttttccctga tcgttaacca
     1021 gatcacgcgc ttcaatcatc atcaccacta atgggtaata atcgacccga tgctgacggc
     1081 accattgcaa cattaaagca tccgcctggg tggagggata ctcgcgccag tagtaacaaa
     1141 gttctgccag caggctttcg ccttctccac aatagacgcg ttgtgcgtat tcataataac
     1201 gcgcatcttc attatcaggt tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg
     1261 ggattcccag gcggcttgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat
     1321 cggggataat cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg
     1381 accaaatccg agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca
     1441 gatgcagaat ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt
     1501 ccagttcttc ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg
     1561 ctaataacac cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg
     1621 accagcgtaa ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct
     1681 gccaggcgga gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca
     1741 gaatttcatg ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact
     1801 gcgcaatccg tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg
     1861 tttctggatg aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag
     1921 tcgtttcctc tatatcgagg atcttccagc aatttttcat aagtcgctca tatccttaag
     1981 cgtttccggg catgtggggt agtcagccag tggttgccag gacaaggtag ggtaatacgc
     2041 atcatcactg gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg
     2101 cgctaagaat tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg
     2161 accaaaggta taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag
     2221 atgaatacgc gacaggatcc atagactttc ttcttcactc aaccagttat taagtacgcc
     2281 cattcgactt aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca
     2341 gcattcggct gtgctggcga caaattgcgc ataagcctga ccttgctcat ttaattcagc
     2401 aagatagtca cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa
     2461 ccgagcggca tgaccgttct cgatcattcg gtgaattgtg cgcagcaggt catcacggtc
     2521 atcaattccc catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttggggga
     2581 tgaatgcgaa ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa
     2641 tattttttgc gaccccatgt ccatataatt tatccttaaa cattcattta atgaatgcaa
     2701 actacatttc gagactcagt tatttatact tttctgacgc aacataatac gcataaaata
     2761 aggattacgc aaaaatggaa cacataaaaa tataatatat tttatctaca accataagga
     2821 aatgataata atttttacgt caagttgctg cgataattta tcaatagatg agattatcga
     2881 acgtgctgaa aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga
     2941 tagcgcaatt gactctccgg acgacgaaaa ggccttttac tggctgaagc tggccgctga
     3001 gcaaggtcat tgtgaagcac agtattcctt agggcaaaag tataccgagg ataaaagccg
     3061 tcataaagat aatgagcaag ccatcttctg gctgaaaaaa gctgccctac aaggccatac
     3121 tttcgcttcc aacgcccttg gctggatact ggatcgtgga gaagacccca actataaaga
     3181 agcggttacc tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct
     3241 tggatggatg tacagaaatg gcaacggagt cgcacaagac tatacgctgg catttttctg
     3301 gtacaaacaa gctgcattac aaggccatag ttacgcacaa gacaatctgg ccgatcttta
     3361 taaagacgga gaaggcgttg ctcaaaacaa gacactcgcc gcattctggt atttgaaaag
     3421 cgcacagcag ggtaatcggc acgcccagtt tcaaattgca tgggattata acgctggcga
     3481 aggggtggac caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcagga
     3541 aagcgtcggc gcttacgtca acatcggtta tatgtataaa cacggacaag gcgttgagaa
     3601 agattatcag gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc
     3661 ctggtataac ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca
     3721 ggctctcgac ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat
     3781 tcgtgagact gaagctttac tgtagaaata aaaacaaggg ccggggaatt tcattctgcc
     3841 catattctct ttattgctat gcgaaatcag actattacgg ctcaaacggg cgtctctgga
     3901 actggtcata accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat
     3961 ggaagtgaca tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa
     4021 ccccatgatg attgaggtcc tggaaaactt cgcgccattc aagctgcgtt ttatcggtga
     4081 tgtctgccag ctcctgccac aagctttctt taatcacccg cataaagacg ctgtcagatt
     4141 cttctttcag gctctcttca tagctcatgg cgaactcttc caggtcacgt ctgacagctc
     4201 gcgtcagctc atcgatctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg
     4261 cctgttccac cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ttcagtgacg
     4321 cgaccagttc acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa
     4381 ctgactataa taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt
     4441 aaattaatgt acgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt
     4501 gacgcggacg tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt
     4561 agccgtattg aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat
     4621 agaatccaaa gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga
     4681 gagcaaagag aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat
     4741 gggccacgta cgtaactaca ccatcggtga cgtgatcgcc cgttaccagc gcatgctggg
     4801 caaaaacgtt ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc
     4861 ggtgaaaaac aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa
     4921 ccagctcaaa atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc
     4981 ggaatactac cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata
     5041 taaaaagact tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca
     5101 agttatcgac ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca
     5161 gtggtttatc aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga
     5221 tcactggcca gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt
     5281 ggagatcacc ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc
     5341 ggacaccttt atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa
     5401 agcggcggaa aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt
     5461 tgccgaagct gaaatggcga cgctggagaa aaaaggcgtc gatactggct ttaaagctgt
     5521 tcacccatta acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta
     5581 cggcacgggc gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc
     5641 taaatacggc ctgaacatca aaccggttat cctggcggct gacggctctg agccagatct
     5701 ttctcagcaa gccctgactg aaaaaggcgt gctgttcaac tctggcgagt tcaacggtct
     5761 tgaccatgaa gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga
     5821 gcgtaaagtg aactaccgcc tgcgcgactg gggtgtttct cgtcagcgtt actggggcgc
     5881 gccgattccg atggtgacgc tggaagacgg taccgtaatg ccaaccccgg acgaccagct
     5941 gccggtgatc ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga
     6001 tccggagtgg gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt
     6061 cgacaccttt atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga
     6121 aggtatgctg gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg
     6181 tatcgaacac gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga
     6241 tgcaggcatg gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct
     6301 ggcagatgcc ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga
     6361 tgctatcgtt gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca
     6421 tgaactggtt tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc
     6481 gcaggtgatg gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc
     6541 tccggctgat atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct
     6601 gaaacgtgtc tggaaactgg tgtacgagca cacagcaaaa ggtgatgttg cggcactgaa
     6661 cgttgatgcg ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaactatcgc
     6721 taaagtgacc gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat
     6781 ggagctgatg aacaaactgg cgaaagcacc gaccgatggc gagcaggatc gcgctctgat
     6841 gcaggaagca ctgctggccg ttgtccgtat gctcaacccg ttcaccccgc acatctgctt
     6901 cacgctgtgg caggaactca aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc
     6961 tgacgaaaaa gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt
     7021 ccgtgccaaa atcaccgttc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg
     7081 ccaggaacat ctggtagcaa aatatcttga tggcgttact gtacgtaaag tgatttacgt
     7141 accaggtaaa ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg
     7201 gcaacattgt tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt
     7261 gacaccacgc aggttccttc cactatgaag gtcatgatcc tagactcagg cgatccgaac
     7321 gggccattaa gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat
     7381 aaagaaacca cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa
     7441 gataccgcat cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg
     7501 aatgcgaccg tgttgatccc cggccgtgat atctacccga ttagcgccaa agtcttccgt
     7561 tcattcttcg ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta
     7621 aaagagatgt acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct
     7681 gcggatattc gttccgacga agaacagacg tcgaccacaa cggatactcc ggcaacgcct
     7741 gcacgcgtct ccaccatgct gggtaactga tgattcggtt gtacccggaa caactccgcg
     7801 cgcagctcaa tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat
     7861 tgcaggaaag ccaggacgct gttcgccagg tagctgcggc acaaggattc gaagaacacc
     7921 acactttttc cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta
     7981 tgagtctgtt tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg
     8041 cggcgatcaa tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctgttga
     8101 tcgtccgcgg taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg
     8161 cgaatcgcag cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg
     8221 ttgctgtgcg cgcaaaacag ctcaacttag aactggatga cgcggcgaat caggtgctct
     8281 gctactgtta tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc
     8341 tctggccaga cggcaaactg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc
     8401 atttcacccc ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc
     8461 atattcttca gcaactgcgt ctggaaggca gcgagccggt tattttgttg cgcacattac
     8521 aacgtgaact gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgta
     8581 cgttgtttga taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa
     8641 atcgcttaag tcagccgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca
     8701 ccctcaaaca agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt
     8761 tgtgccataa acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct
     8821 ctgtttggcg gcacctttga tccggtgcac tatggtcatc taaaacccgt ggaaacgctg
     8881 gcgaatttga ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt
     8941 ccccagccgg aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac
     9001 aagccattat ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgca
     9061 caaacactga aagagtggcg gcaggaacaa ggaccggacg tgccgctggc gtttattatt
     9121 ggtcaggatt cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat
     9181 gcacatttga tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac
     9241 cagcaatggc tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc
     9301 ggtaaaattt atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa
     9361 cgtttgcaaa acggtgaatc gtgtgaggat ttattgccgg aaccggtatt gacttacatt
     9421 aaccaacaag gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt
     9481 taattcgtca tggcgaaaca caagcgaatg tcgatggtct gtacagcggt catgcgccca
     9541 cacccctgac cgcacgcggt attgagcaag cgcaaaatct gcatacactg ctagatgatg
     9601 tttcctttga cctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg
     9661 ttctcagtga ccgccagctc cccgttcata tcatccctga actcaacgaa atgttttttg
     9721 gcgactggga gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg
     9781 cgtggtgcaa tgactggcag catgctatcc ccacgaacgg tgaaggattt caggcatttt
     9841 cgcaacgtgt ggaacgcttt atcgcaaggc ttagtgaata tcagcactat cagaatattt
     9901 taatcgtcag ccatcagggc gtactgagtc tgttaattgc tcgtttaatt ggcatgcctg
     9961 ccgagtctat gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc
    10021 aaaaattcgc gacgctacgc gtcctcaata gccgtgccat cggggtcgag aatgcatgac
    10081 ttttctgttt ttttacgggt aagccgcaac gaccattgac agccccgggc aggctgatat
    10141 tctccgcagc cagacttttt ccgccagaca cgactttgta gaaattgttt tacaaaaatg
    10201 gcgatgcaat ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga
    10261 caattgtccc gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga
    10321 ttcacccagg gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt
    10381 gatgacctca aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc
    10441 gactgcatga tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac
    10501 gttgtgcagg agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc
    10561 gccgactgga ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc
    10621 cgtcgcctgt atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc
    10681 gtgggaacga aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt
    10741 ccgaaagata tgcccttcga gctgattgaa attccggccg gcaaacgcgg caagaatgcg
    10801 gacatcaagc gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac
    10861 cgcattgtca tcctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag
    10921 ctggaacgct ggaagctgga tggtcgcgac gtaagtctac tgattggcgg gcctgaaggg
    10981 ttgtcgcctg cctgtaaagc ggcggctgag cagagctggt cgctgtcggc gcttaccctc
    11041 ccccatccgc tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc
    11101 accaaccatc cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg
    11161 aaactacaga actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg
    11221 ctggtcgcct ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat
    11281 ctgcaaattg ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg
    11341 gtgcctatcg cgcccagccg cggcattatc tacgaccgta acggtatccc tctggccctc
    11401 aaccgcacta tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg
    11461 ctggacgctt tgcgcagcgt ggtagatctg accgatgacg atattgctgc attccgaaaa
    11521 gagcgcgcac gttcacaccg tttcacctct attccggtga aaactaacct gaccgaagta
    11581 caagtagctc gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat
    11641 aaacgtcgtt actatcctta cggttcggcg ttgacccacg tcatcggata tgtgtcgaaa
    11701 atcaacgata aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca
    11761 acgcatgata ttggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag
    11821 accggttatg aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa
    11881 gtaccaccgc aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat
    11941 attgaaacgc tgctggcggg tagccgcgca gctgtggttg ttaccgatcc gcgtacaggt
    12001 ggggtgctgg cgctggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc
    12061 tccagcaaag attattccgc cttgctgaac gacccgaata caccgctggt gaaccgcgcc
    12121 acacaggggg tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcatta
    12181 agcgccgggg tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca
    12241 gggtcggaaa aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca
    12301 agatcgctgg aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc
    12361 gatcgcctct ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg
    12421 gcggaagaac gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa
    12481 ccgtggtatc agggtgacac cattccggtt ggtatcggcc agggttactg gacagcgacc
    12541 ccaatccaga tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg
    12601 catttgctga tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa
    12661 ccgcccgtcg gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt
    12721 gttgctaacc gccctaatgg tacggcgcat aaatactttg ccagcgcacc gtacaaaatt
    12781 gcggcgaaat ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg
    12841 cacaaaattg ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac
    12901 aatccgcaag tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt
    12961 acgctgatgc gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct
    13021 gcggaaaatc cagcggttgc cgcagcggag gaccattaat catgacggct aatccgaata
    13081 aaaaaacatt ctgggataaa gtccatctcg atcccacaat gctgctgatt ttactggcat
    13141 tgctggttta cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg
    13201 agcgtaaaat cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc
    13261 ctccacgcgt ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg
    13321 tggcggtaga tgctttcgga gccatctcta aaggcgctca acgctggctg gacctcggta
    13381 ttgttcgttt tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct
    13441 ttatcaaccg cgacgtttgc ccgccatcgt tgaagaacac tggcatcgcg ctggtgctga
    13501 tatttatgcc cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg
    13561 cgctttccgg tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg
    13621 cagtagtgct ggtagcggcg ttcattccga ttctatggtt cttcctgatg catgattacc
    13681 agcgccagcg cgtaatgatg ctcctagacc cggaatcaga cccactcggc gcgggctatc
    13741 acattattca gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc
    13801 acggcactca gtcacagctt gaatttctcc ccgaacgcca tactgacttt atcttcgcgg
    13861 tactggcgga agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc
    13921 tgatcatgcg cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg
    13981 ctggtggctt aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg
    14041 gtattctgcc ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa
    14101 ttgtgctgat ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt
    14161 cgaaaagcgt gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca
    14221 ggaatgctcg cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag
    14281 cctgcggtat gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca
    14341 ctgaacgcga cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag
    14401 gatccgtctc gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt
    14461 aacctgacgg cctctggcga agctttcgat ccgacaaagc tgacggcggc ccatccaacg
    14521 cttccgatcc ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg
    14581 cgcattaatg atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca
    14641 gctgaccgtc ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc
    14701 caggatggtt cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttat
    14761 gccctgcctg cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg
    14821 cagggtgaca ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg
    14881 ccggtaacca gtagcggttt cctcggcgct caaacgacct tagcgcctgg tgtactggaa
    14941 ggcagcgaac cgacgcctgc gccacagccc gttgttacag cttcgtcgac aacgcctgca
    15001 acctcgcctg caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg
    15061 gtgcaagtcg gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga
    15121 cagaagttcg gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt
    15181 ggcccattcg ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc
    15241 caattacagt catttattac taccgcgcag tagcgtaaag caggcatctg atgtgtcaat
    15301 ttctgtaaat gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt
    15361 gctatagtag ggcacttttt taattccatc acggatgtcg tagttcagac catgaatacc
    15421 attttttccg ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt
    15481 atctctgctg cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag
    15541 atcgatgcgg agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag
    15601 aacgcagatg tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc
    15661 ggccaggcaa tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac
    15721 gcatgggcca ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc
    15781 atgcaggttc cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct
    15841 tgtgtcgcca tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac
    15901 agctacgtta acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat
    15961 gctgatggtc agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt
    16021 gacgtaccga atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc
    16081 cagctgaacc gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc
    16141 ggacacactg acaaagcagg ttacaacctt gttgcttctg cgactgaagg ccagatgcgc
    16201 ttgatttctg cggtgatggg tggacgtact tttaaaggcc gtgaagccga aagtaaaaaa
    16261 ctgctaacct ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag
    16321 ttcgcctcag aaccggtttg gtttagtgat tctgatcgcg cttcgttagg ggttgataaa
    16381 gacgtgtacc tgaccattcc gcgtggtcgc atgaaagatc tgaaagccag ctatgtgctg
    16441 aacagcagtg aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc
    16501 cagcttgatg gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa
    16561 ggtaacttct tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt
    16621 taaaaattga acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa
    16681 actcccacct tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa
    16741 ccaaacttaa cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg
    16801 cgttacctga gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact
    16861 acaccccaac ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca
    16921 acgccactca tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg
    16981 tccgcatggt tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactctcctc
    17041 tcctcgctgt gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg
    17101 tatcaggata aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccgg
    17161 gctatgcatg aattcaccga tacccgcgat aatagtaccc ttgatgaaat ctggctggtc
    17221 gagcactatc cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg
    17281 ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccggga
    17341 caacaggtga tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg
    17401 gtgaccttgc ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat
    17461 cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta
    17521 cgaattcgac gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca
    17581 ccatttttac gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa
    17641 tggaaacccg aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg
    17701 ctactaaaca atccggactt cgaatatatt accgcttaat tccatacatc aatggcccaa
    17761 tttacactgg gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta
    17821 tattttcttt atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt
    17881 gattaaccta ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat
    17941 aagattattg taatttttta aatattattt ttccatgaat aaaattggag taagtgcgtg
    18001 gatagtaata atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa
    18061 atatttacaa ccttacgaaa tattgatctt aaccttctga ctatttttga agctgtatat
    18121 gtacataaag ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt
    18181 cagtctattc agaaactgcg cgttatattc cctgacccat tgtttattcg caaaggccag
    18241 ggtgtcactc ctaccgcatt tgcgatgcat ctacatgagt atatcagtca gggccttgag
    18301 tccattcttg gcgcgctgga tatcgaagga agctatgata agcaacgaac gataactatt
    18361 gcgaccactc cctcggtcgg agccctggtc cttcctgtca tctatcgggc gattaaaact
    18421 cactatccgc agcttttact gcgcaacccg cccatcagcg acgcggaaaa ccaactcagt
    18481 cagtttcaaa ccgatctcat catcgataac atgttttgca ccaatcgtac ggtgcaacat
    18541 catgttctgt tcaccgacaa tatggtgtta atttgccgca agggaaatcc actactctct
    18601 ttagaagatg acagagagac tatcgacaac gctgcgcatg tacttctgtt accggaaggg
    18661 caaaatttca gcggtctgcg gcagagagtt caagagatgt ttccggaccg gcaaatcaat
    18721 ttcaccagct acaacatttt gacaatcgct gcactggttg ccaacagtga catgttagcg
    18781 attattccca gccgttttta taacctgttt agtcgttgct ggccgctgga aaaactgcct
    18841 tttccgtcct taaatgagga gcaaatagat ttctctatcc actacaacaa attcagcctg
    18901 cgtgatccca tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt
    18961 gttacagcca gatcacagtg cagaacgata aaatggcaca atccaacaac aattttacat
    19021 tttcgcgacc gctttggctg ctttcaggtc cgtttcaatg atatactgcc agtcgttaat
    19081 tcaaaaatag ttgataatta caacaatcta ttgaattgaa acgctttcct tcgtaattcg
    19141 caactggaac acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg
    19201 cgatgccgat aagatggccc ttatcccggt taaaaacgtg gcaacagagc gcgaagccct
    19261 gctgcgcaag ccggaatgga tgaaaatcaa gcttccagcg gactctacac gtatccaggg
    19321 catcaaagcc gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag cctcctgccc
    19381 taacctggcg gaatgcttca accacggcac agcaacgttt atgatcctcg gcgctatttg
    19441 tacccgccgt tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa
    19501 tgaaccagtg aaactggcgc agaccattgc cgatatggcg ctgcgttatg tggttatcac
    19561 ctccgttgac cgtgatgacc tgcgcgatgg cggtgcccaa cactttgcgg attgtattac
    19621 tgccattcgg gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg atttccgcgg
    19681 tcgaatggat cgtgctctgg atattctgac tgcaacgccc ccagatgtgt tcaaccataa
    19741 cctggaaaac gtaccgcgta tttaccggca ggtacggcct ggcgcagatt acaactggtc
    19801 gctgaagctg ctggaacgct ttaaagaagc gcatccggaa atcccgacca agtctggtct
    19861 gatggtgggt ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg acctgcgccg
    19921 tcatggtgtg acgatgttaa cgctggggca atatttgcag ccaagccgcc atcacctgcc
    19981 ggttcaacgt tacgttagcc cggatgagtt cgacgaaatg aaagccgaag cgctggcgat
    20041 gggctttacc catgctgcat gcggtccgtt tgtccgctct tcttaccacg ccgatttgca
    20101 ggcgaaaggg atggaagtta agtaatcctg taataattac ttacatctgt ctgataaaaa
    20161 acccgctttt tgaagcgggt tttttgtatc gaacaagata ttgagggagc gtcctgctcg
    20221 ccacgtcact ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc
    20281 gccgcagcat cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct
    20341 ccgcccagcg tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactacc
    20401 agcagtttgg taatactaat ctcacccata gataccttct tgacatataa ccggcaacag
    20461 aacgcgcctt aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat
    20521 ctgtaacaag gtgaatcaag cgccgagtaa aaaaacatca taataattgc ggcggcgcaa
    20581 agcgacggtt gtttaagatg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca
    20641 cttccgccat aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat
    20701 ctataatccg gctttgcccg atatttttgt tcccgcactc ccccgccgcc accatataac
    20761 aggtggtatc cagcgcacga gcggcaagca acgttgacca gtgatgctct ttgagcggcc
    20821 cacgaaccca ggcggcagga agtaccagga tttcagctcc ctgtaaagcc tgtgccagcg
    20881 ccagctctgg aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccttccacct
    20941 ccagtaacgg agctatttca ttaccagcat caacatggcg tgattcctga atggcaaatg
    21001 catcatagag atgcagtttg gcataacggg cgacgatgtt tcctgcctga agtgccacca
    21061 gcatattcca tgcgcgcccc ggcgttgaag gaagatgaat cgtcagaatt gtcgtcatca
    21121 tgttacgttt actttctcgc cgtaaacgtc cgaggaattc gccttccagc agctgtgctg
    21181 atttaaccga tagatctgca tcatgatcat cgcgcgccag caatgcttcc ggcaggacaa
    21241 acagcgatac gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct
    21301 tttcccacac agatgtaaca gcaaactgtc ctgcagcaac cagcattgcc acctcctgtc
    21361 agagtcgtaa aattcgccaa cgacgattat tcggcgttac acttatcact catacaaatc
    21421 aaatagcagg attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg
    21481 ggaagcgtag cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg
    21541 ttggggacgc tggcagcaaa cctgattggg gcattcatca taggaatggg attcgcctgg
    21601 ttcagcagga tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt
    21661 ggcggtctaa caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc
    21721 cgctttggct gggcattact gaacgttttc gtcaaccttc tggggtcttt tgccatgacc
    21781 gcactggcat tctggctgtt ttcggcctca accgcacact aaagaccaaa aaaaacccgc
    21841 tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca
    21901 gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt
    21961 ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc
    22021 ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta
    22081 gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgtgtcggt tatcagtaca
    22141 ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat
    22201 ttttgatgtt gcttatttta gttattgatt ttaaagaagt tactaaaact tcatttgcct
    22261 caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg
    22321 taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg
    22381 agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa
    22441 tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt
    22501 tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt
    22561 tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt
    22621 ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa
    22681 tgttcaggct gttgccaggt ttgtgcaata ttttctctaa acgttgtcat cctctcatct
    22741 gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg
    22801 acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca
    22861 tagcaaagct acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc
    22921 tgctctattt atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg
    22981 cgtcgttttc tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa
    23041 atgcaatcaa ggaaaaatta ttttattttt ttaacgagat acaacaatca tcttaacgaa
    23101 gtatataata tatatcgtca acccatgctc atattagatt attttttgag cgcacagccg
    23161 cagctaacac cattgcaatt aacaaatttg catcaatcca ccatcaattt acacacatta
    23221 ttatgtgata attgccaacc gctaaatatg cgttttgtta tctatgtata aaaacagcaa
    23281 cttcaatgtc ttaatggcag tttttcttga ttttaatcag cattcatcgc caatttattg
    23341 ggcatatttt ttccttaagc tttaggaagt ttttattatt tactttgggc ctggagacag
    23401 gaaaaattat gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag
    23461 ctcgctacat cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat
    23521 tgctgattat cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag
    23581 gctacagcgc cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg
    23641 acctcggcat gatgattatg atgctgtgtg gctttgccgc ttacatgacc catatcggcg
    23701 cgaatgatat ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc
    23761 tgctgatgat tgccgcctat tttgttgctt gtctgatgtc actggccgtc tcttccgcaa
    23821 ccggtctggg tgttttgctg atggcaaccc tgtttccggt gatggtaaac gttggtatca
    23881 gtcgtggcgc agcagctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt
    23941 caggggatgt ggtgctggcg gcgcaggctt ccgaaatgtc gctgattgac ttcgccttca
    24001 aaacgacgct gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct
    24061 ggcaacgtta tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa
    24121 tcaccaccac tgcccccgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac
    24181 tgatttttga cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta
    24241 tgctgattgc ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg
    24301 gtctggaagt ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg
    24361 ttgccgctgg ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt
    24421 ctatcgctac ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga
    24481 ccatgctggc ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga
    24541 tgatcccgaa actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc
    24601 tgcaggcgtc aaacctgggt cgtaccctat cacccgtttc tggcgtagtc gttgcggttg
    24661 ccgggatggc gaaaatctca ccatttgaag tcgtaaaacg cacctcggtg ccggtgcttg
    24721 ttgggctagt gattgttatc gttgctacag agctgatggt gccaggaacg gcagcagcgg
    24781 tcacaggcaa gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc
    24841 actgtggtat atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag
    24901 atggcggctg gcgcaatatt caagataacg cctggcagtg gtgcggctga tggtcagtgc
    24961 ctgcgccacc gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc
    25021 gtttagcgtc aaggggtcaa tgccggtcgg cagttcgtct ttgggttcac cgcgagcata
    25081 ggcattaaac atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg
    25141 tttacactgg cggaaacggg ttagcgtttg ccccagtgta ttgattcact tgaagtacga
    25201 aaaaaccggg agaacattgg attattcggg atctgatggg attagatttg gtggggcttg
    25261 caagcctgta gtacaaattt taggcgttaa ccaatgaaac gcgaaagata gtaaaaaatt
    25321 gcttttgttt cattgaaaaa acgaaaaaca aaaac
//
LOCUS       DAJTNM010000048        24486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTNM010000048 DAJTNM010000000
VERSION     DAJTNM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.048"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            62..343
                     /locus_tag="OO172_002088"
     CDS             62..343
                     /locus_tag="OO172_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5512279.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            309..1386
                     /locus_tag="OO172_002089"
                     /pseudo
     CDS             309..1386
                     /locus_tag="OO172_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1779..2120)
                     /locus_tag="OO172_002090"
     CDS             complement(1779..2120)
                     /locus_tag="OO172_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5512280.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2133..3005)
                     /gene="copD"
                     /locus_tag="OO172_002091"
                     /pseudo
     CDS             complement(2133..3005)
                     /gene="copD"
                     /locus_tag="OO172_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3009..3383)
                     /gene="yobA"
                     /locus_tag="OO172_002092"
     CDS             complement(3009..3383)
                     /gene="yobA"
                     /locus_tag="OO172_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5512281.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3522..3752
                     /gene="holE"
                     /locus_tag="OO172_002093"
     CDS             3522..3752
                     /gene="holE"
                     /locus_tag="OO172_002093"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5512282.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3854..4510
                     /locus_tag="OO172_002094"
     CDS             3854..4510
                     /locus_tag="OO172_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR5512283.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4534..5196
                     /gene="exoX"
                     /locus_tag="OO172_002095"
     CDS             4534..5196
                     /gene="exoX"
                     /locus_tag="OO172_002095"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5512284.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5193..7253)
                     /gene="ptrB"
                     /locus_tag="OO172_002096"
     CDS             complement(5193..7253)
                     /gene="ptrB"
                     /locus_tag="OO172_002096"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5512285.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7462..8121)
                     /locus_tag="OO172_002097"
     CDS             complement(7462..8121)
                     /locus_tag="OO172_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5512286.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8448..8804)
                     /locus_tag="OO172_002098"
     CDS             complement(8448..8804)
                     /locus_tag="OO172_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5512287.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8871..9161)
                     /gene="yebG"
                     /locus_tag="OO172_002099"
                     /pseudo
     CDS             complement(8871..9161)
                     /gene="yebG"
                     /locus_tag="OO172_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9295..10473
                     /gene="purT"
                     /locus_tag="OO172_002100"
     CDS             9295..10473
                     /gene="purT"
                     /locus_tag="OO172_002100"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5512288.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10528..11169)
                     /gene="kdgA"
                     /locus_tag="OO172_002101"
                     /gene_synonym="eda"
     CDS             complement(10528..11169)
                     /gene="kdgA"
                     /locus_tag="OO172_002101"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5512289.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11206..13017)
                     /gene="edd"
                     /locus_tag="OO172_002102"
     CDS             complement(11206..13017)
                     /gene="edd"
                     /locus_tag="OO172_002102"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5512290.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13252..14727)
                     /gene="zwf"
                     /locus_tag="OO172_002103"
     CDS             complement(13252..14727)
                     /gene="zwf"
                     /locus_tag="OO172_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5512291.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15065..15934
                     /gene="hexR"
                     /locus_tag="OO172_002104"
                     /gene_synonym="yebK"
     CDS             15065..15934
                     /gene="hexR"
                     /locus_tag="OO172_002104"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5512292.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16062..17504
                     /gene="pyk"
                     /locus_tag="OO172_002105"
     CDS             16062..17504
                     /gene="pyk"
                     /locus_tag="OO172_002105"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5512293.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17635..18606)
                     /gene="lpxM"
                     /locus_tag="OO172_002106"
                     /gene_synonym="msbB"
     CDS             complement(17635..18606)
                     /gene="lpxM"
                     /locus_tag="OO172_002106"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5512294.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18726..20048)
                     /gene="mepM"
                     /locus_tag="OO172_002107"
     CDS             complement(18726..20048)
                     /gene="mepM"
                     /locus_tag="OO172_002107"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5512295.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLLFD"
     gene            complement(20064..20996)
                     /gene="znuA"
                     /locus_tag="OO172_002108"
     CDS             complement(20064..20996)
                     /gene="znuA"
                     /locus_tag="OO172_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5512296.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21075..21830
                     /gene="znuC"
                     /locus_tag="OO172_002109"
     CDS             21075..21830
                     /gene="znuC"
                     /locus_tag="OO172_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5512297.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21827..22612
                     /gene="znuB"
                     /locus_tag="OO172_002110"
     CDS             21827..22612
                     /gene="znuB"
                     /locus_tag="OO172_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5512298.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22759..23769)
                     /gene="ruvB"
                     /locus_tag="OO172_002111"
     CDS             complement(22759..23769)
                     /gene="ruvB"
                     /locus_tag="OO172_002111"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5512299.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23778..24389)
                     /gene="ruvA"
                     /locus_tag="OO172_002112"
     CDS             complement(23778..24389)
                     /gene="ruvA"
                     /locus_tag="OO172_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5512300.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         6044 a         6232 c         6038 g         6172 t
ORIGIN      
        1 tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga gaggtatggc
       61 aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata acccaccatc
      121 aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac ctgaactaca
      181 cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata aggttgatac
      241 cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt taatggagaa
      301 gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta acatatcgaa
      361 ggagagacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt acactaggta
      421 aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat atatacaaaa
      481 actactctcc tgctgcccta attgaaaagc ttaaaggggt cgactcattt actatggcag
      541 actggattga acgttacaaa acgattctta taaggagaaa agcgtccaga aatacttata
      601 aaactcgggt aaatcaactg gtgacaataa aagaaaaatt aggaggggtt ttactgacag
      661 aaataaccac tcgccatatc gccgagtttc ttgatttgtg gattgaagga gggaaaaaca
      721 cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaagcc attgttgaag
      781 gacgtatatc tcaaaatcca gtaacgccaa caagagcacc gaaaatagta gttacaagag
      841 aacggctgaa actaaagaca tacaactgca tcagggaggc agcagatcaa cttccggcat
      901 ggttcccatt agctatggac ttagcccttg taacaggaca acgtcgcgaa gacataacga
      961 atatgcggtt cagtgatatt tatgatgatc gtctccacat caggcaaatt aagacaggaa
     1021 tgatgattgc tatccccctg tcactcagcc ttcctgtcgc tggtttacga cttggtgcag
     1081 tagttgaaca gtgccgcatg gtaagtaggg ggggattatc taatcagtgc cgggattaga
     1141 aaaaacagcc ctgacggcag cattcacccg gacggcctga caaagaaatt tgtcgcagcc
     1201 agaaaattaa caggtatcct gttcagtgaa aacccaccaa cttttcacga aatcagaagc
     1261 ctggctggac gattgtacaa agaaatatgt ggagaagaat ttgctcagcg tctacttggc
     1321 cacacatcgg agaagacaac aaaaatgtat cttgatgaga gagaaaaaac gtacttactg
     1381 ctctgatttt aacgtaaatg gattgttaaa tgtattttgg ttgtgatata accaaaaaag
     1441 accggaatac agaaattcga gtaaatttcg gggaatttcg gggagacgtt tgcaactaat
     1501 tgattttaaa tacaattaaa aaaagaccga atacgattcc tgtattcggt ccagggaaat
     1561 ggctcttggg agagagccgt gcgctaaaag ttggcattaa tgcaggctta gttgccttgc
     1621 cctttaagaa tagatgacga cgccaggttt tccagtttgc gtgcaaaatg gtcaataaaa
     1681 agcgcggtgg tcatcagctt aaatgttaaa aaccgcccgt tctggtgaaa gaactgaggc
     1741 ggttttttta ttggaaatca aaaggctatt ttaggtaatt aacagagttt ttcagctcgt
     1801 tctataaacg gtgccagact cattttttcg ccgggattgt taggatcatc aatctgaatc
     1861 accgaaatgg gttgggcatt ggtcttccca ctggcaactt ccttttgtgc gatatcgttt
     1921 aaaggatact gcacgagggt acttgggttg atgacataca aagcattacc cggtcggcaa
     1981 gtcagcatta cctcttcgcg attaaacgcc catttgtctt tacccacttc aaaacgactc
     2041 acggttatca cctgtggagc cgccagcgcc gctgcagaac tggtgagtaa cagaaacgcc
     2101 agaatacttt ttttcatcat tttcgtaatc catcaaaaag gttcgagagt cgcaagcagg
     2161 ctgatgatca ccagcactac cgcacctatc gcccattcga gtttagtcat ccacacaaac
     2221 cagggtgccg cacgatcttc atcctgcctc atgcgcggta caagaacata acgattagcc
     2281 agcgcaatga ccaccataat catcaccagg atggctttga gcaataagag ttggccctag
     2341 taagtcgtaa gcgtgggggg aaatccggta atcaacaatg cattaagcac gccgcttgcc
     2401 agtacgccga tcaccgcaaa atgcccgcac caggaaaaac gcatcagcgc ctgaatagcc
     2461 tggtgtcgcc aacggccttt gatgagttgc atacaccaga gcaccggcag taatccccca
     2521 aaccacgccg ccgcacaaat caggtgaatc gcgtgattag tctgatggat tttcgctgtt
     2581 accccttcat tcagcgtcgc atgccccact cccgccagca gaataaattg cgcggtagtg
     2641 agcataaaca acaaacgtgg catattccgc ggttgcataa gggcgacgat caacgttacg
     2701 agggcgagaa caatttgcca taaccagacg ccaccaaact gcgtttgcaa aacagcctgc
     2761 caaatgtttg gcgaaaatac atctgtccat cccgttccca tcaacccgcc ctgaacggcg
     2821 agcattgcag tggcactgat caaactccag acggcagcgt gctgttgtaa acgcagagaa
     2881 cgcttcgtca gcaagcgacg aatcattaag ggggccagcc aggcaccata cattgcaaag
     2941 ccaaagacca gcatcaacga ggtgaaatgg ataaatcgca gcgcgatcca ggtaaacgcc
     3001 agcatgattt atttcacgct aaaggtgtag tgtcctttcg ttttatgccc gtccaccgaa
     3061 acaacatgcc agtcaacggt gtaggttccg ggtttcagcg aatcggccag tggaacaatc
     3121 agttgctttt ggtcctgctc attccgcttc gctggtaatg ttttaatatt ttcgtttttt
     3181 ggccccgtga tttttgcacc actgaatcct gtttcaacgc cttccgagaa gtttaaagtg
     3241 attgcctgcg gggcagctgt cacttgcgcg tttgccgcag gatactgatg cgttaaatgc
     3301 gcatgtgccc agacggaagg tgtcaccaac gaagtggtca ggattgccag cgcgtagcga
     3361 agggagcgtg cagttgaagc catattatct attccttttt gtaataactt ttttacagag
     3421 cataaccttg tctaatgtct gagtcgagga tcatcaattc cggcttgcca tccaggctca
     3481 ctcttagtaa cttttgcccg cgaatgatga ggagattaag aatgctgaag aatctggcta
     3541 aactggatca aacagaaatg gataaagtga atgtcgattt ggcggcggcc ggggtggctt
     3601 ttaaagaacg ctacaatatg ccggtgatcg ctgaagcggt tgaacgtgaa cagccagaac
     3661 atttgcgcag ctggtttcgc gagcggctta ttgcccaccg tttggcttcg gtcaatctgt
     3721 cacgtttacc ttacgagccc aaacttaaat aaaacttata cagagttaca ctttcttaca
     3781 taacgcctgc taaattatga gtattttcta aaccgccccc ataatttgca gtcattttga
     3841 aaaggaagtc attatgtcgt tctggaaagt tgcagcagcg caatatgagc ctcgtaaaac
     3901 ctcactcacc gaacaggtag ctcatcatct ggagtttgtg agggccgccg ccaggcaaca
     3961 atgtcagctg ttagtttttc catcgctttc tttactgggg tgtgattatt cgcgacgtgc
     4021 ccttcctgcc ccacccgatc tttcactgtt agacccgctt tgttatgccg caacgacctg
     4081 gcgaatgacc atcattgccg gccttcccgt tgaatataac gatcgcttta ttcgtggaat
     4141 tgcggtgttc gcaccgtggc gaaaaacgcc tgggaactac catcagagtc atggtgcatg
     4201 tctgggccgt cgttccagaa ccatcacggt ggtcgatgaa caaccgcaag gtatggatat
     4261 ggacccgacc tgttcactgt ttacgacagg gcaatgtctg ggagaacccg acctgctggc
     4321 gtcggcccgt cgcttacaat ttttttcaca tcagtactcg attgccgtgc tgatggcaaa
     4381 tgcccgtggt aacagtgcac tatgggatga atatggtcgt ctcatcgttc gcgccgatcg
     4441 tggttcatta ttgttagtcg gtcagcgttc atcacagggt tggcaaggcg atatcattcc
     4501 attacgctag gctttttcgg cctggagcat gccatgttgc gcattatcga tacagaaacc
     4561 tgcggtttgc agggagggat cgttgagatt gcctctgttg atgtcattga cggaaaaatc
     4621 gtcaacccca tgagcctcct ggtgcgcccc gatcgtccta ttagtccaca agcgatggcg
     4681 attcatcgca tcaccgaagc catggtcgcc gataaaccgt ggattgaaga tgtgatccca
     4741 cactattacg gtagtgaatg gtatgtcgcg cacaacgcca gctttgaccg ccgcgtactg
     4801 cctgagatgc ccggtgagtg gatttgcact atgaaactgg cccgtcgttt gtggccaggg
     4861 ctcaagtaca gcaatatggc gttatataaa acacgcaagc tcaatgtaca gacaccgccg
     4921 ggcctgcatc atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt gcttatcgat
     4981 attatgaaca cctccggctg gacggcagaa cagatggccg atatcaccgg acgtccgtcg
     5041 ttgatgacga ccttcacctt tggcaaatac cgtggcaaag cggtttccga cgttgccgaa
     5101 cgcgatccgg gctatctgcg ctggttattt aataacctgg acagcatgag cccggagctg
     5161 cgtttaacac tgaaacatta tctggaaaat acttaatccg caggcgtagt gggtaatgtt
     5221 ccctgcgcca gcgcgaccag aaaagcatat tccatcgcta cgccttcgta cgatttaaag
     5281 cggccagatt taccgccatg gcctgagtcc atgtcggtac agagcaataa aagatggtca
     5341 tcggttttca gctcgcgcaa tttagcgacc cattttgccg gttcccaata ttgcacctga
     5401 gaatcgtgta aaccggtcgt taccagtaaa tgcggataag cctgtgcggt gacgttgtca
     5461 tacgggctgt agcttttcat gtactcgtaa tattgcggat cctgcggatt tccccactct
     5521 tcaaactcac cagtggtaag aggaattgat tcatcaagca tcgttgttac aacatcaaca
     5581 aacggtacct gggcgataac gccgtggaat aattccgggc gttgattaat tgcaacgccc
     5641 atcaacatgc cccccgcact tccgcccatc gcataacaga gcgaaggaga gccatagccc
     5701 agttttaaca atgcatcgca ggcatcaaga taatcattaa acgtattttt cttcttcaga
     5761 aatttaccgt cttcgtacca ttgttgcccc agctcaccac cgccgcgaac atggacaatg
     5821 gcgtagacaa agccacgatc taacaaactc aagcggctaa aactgaaatc ggcatcaata
     5881 cttgcgccgt aagaaccata gccatacacc agcaacgggt tgtgtccttt gcgaaaatgt
     5941 ttgcgatgat agaccagcga aaccggaact tcgacgccat cacgggcgac tatccacagg
     6001 tgttcactgc ggtaattcgc cgcatcaaaa ccgggcactt ctgtttgttt taatacacga
     6061 cgctcaccgg tatccatatc cagttcaaac aaagtgtctg gtgtggtcat ggaagaataa
     6121 ccataacgca atcgcgcggt ttcaggttct ggattgtagg caatccaggt cacataggcc
     6181 ggatcatcaa aggcaatgcc aatgacttcc cgggtcttgc ggttaatttg gcgcaaactg
     6241 gttaacccgc gctgacgctc ttcaaccacc agccagtcgg taaacagcgt aaacccttcc
     6301 agcatgatgt tttcacgtgg cggaattaac tcttcccact gttgctcatc acgcatacgg
     6361 gtacggtata agccaaagtt tttgccgtgg cgattggaac gcagataaaa acgatgctgg
     6421 tagtgatcaa ggctgtattc gtgatctttg cggcgcggca gaaaaacaaa cggctcggca
     6481 tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc cagatgaatg
     6541 acgacatagt gcttcgacgt cgttttatgc aggctgacat aataggtatc gtctttttct
     6601 tcgtagatca gtgtatcttg cgatgctggc gtaccgatgg cgtgacgcca gacctgataa
     6661 ggcagcagcg tcaccggatg cttgcgaaca tagtagaaag tccaggagtc atttgcccag
     6721 acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttacctgt ttccagatta
     6781 cgaaaacgaa tgccgtactg gcgtcgggaa agaaaatctt ctgccagcgc cataatggtg
     6841 ttatcgggcg taatcgccat tccgcccatc gaataaaact cactatgagc tgcgcgcttg
     6901 ttggcatcga gcaatgtttc ccactcatcc cactcttcgc tgaatgccga ttgacgctgg
     6961 tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc attttttatg
     7021 tagggcgcag aaacttctct ttgcggaatg cggtcgatga tttcctttaa gatgcgatcc
     7081 tgcaaggctt gttgtgaggc catcacccga tgaccgtaac tattttcttg ttgcaggtag
     7141 tccaggactt ctggctgaga acgcgtatcg tcccgcagcc agtagtaatt atcgatgcgc
     7201 gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag catgttattg
     7261 ttctttctgg ttgaaacatc ttataagggt ggcaaaactc accggggatg caagcgaaac
     7321 agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg ttatgcagac
     7381 agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa tagtcaactt
     7441 caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt cgagatcgcg
     7501 ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg cattcaggta
     7561 actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat agatttccag
     7621 cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg gttgttcgat
     7681 tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca attgctggtc
     7741 gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa agaccagcgc
     7801 aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa actgcggttc
     7861 gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg tacccgctac
     7921 cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt tacgtgctga
     7981 tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctggcacta acagtttgcc
     8041 caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca gggattgcag
     8101 ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc ctgtagcata
     8161 gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata agcggggcgc
     8221 taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat caggtgataa
     8281 gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg ctgattgccc
     8341 gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg gacggacaag
     8401 gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta acgccgctga
     8461 tattccgcca ttcccgcttt gcagtccacg ctgacctggt aatgaatatc ggcactttta
     8521 ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc ctgcaaactg
     8581 acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca gcgcgccacg
     8641 cgattttgtt gataatcacg ttttacgctc gcggcaattc cggtagcatc cagaccttca
     8701 cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac tgatgcgcag
     8761 gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt ctccatagca
     8821 caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct tcaggccgcg
     8881 tgtgaggcat cttcctcttc ggattcagca ccgacgacca gtggggacgg taatttgccg
     8941 gttttcagaa tggtgctcag catatctttt tgttctgcca gccatagcga aagggcttca
     9001 cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag cagatccgcc
     9061 gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga cattttctct
     9121 tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct ctcataataa
     9181 ctgtgatttt atacagtata tttcttttcg gttgagaaat caacatcagc aataaagaca
     9241 cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt cgttatgacg
     9301 ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg ctccggtgaa
     9361 ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat tgccgtcgat
     9421 cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat taatatgctt
     9481 gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat cgtgccggag
     9541 atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact gaatgttgtc
     9601 ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg cctggcggca
     9661 gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag ccttttccgc
     9721 gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag ctcttccggc
     9781 aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga atacgctcag
     9841 caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa gtttgacttc
     9901 gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc accagtaggt
     9961 catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat gagcccgctt
    10021 gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg cggttatggt
    10081 ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga ggtctcccct
    10141 cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga gtttgccctg
    10201 catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg tcctgcagct
    10261 tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa tgtgcagaat
    10321 gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga tggcagccgt
    10381 cgtctggggg tggtactggc tacttcagag agtgttgttg acgccattga acgcgcgaag
    10441 cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaacgct acaaaaatgc
    10501 ccgatcctag atcgggcatt ttgactttta cagcttagcg ccttctacag cttcacgcgc
    10561 cagcttagta atgcggtcgt aatcgcccgc ttccagcgca tctgccggaa ccagccagga
    10621 accaccgatg cacagcacgc ttttcagtgc caggtagtca cggtagttag ccggagaaat
    10681 accacccgtc gggcagaaac ggacctggga gaacggaccc gcgatcgcct gcagggcttt
    10741 cacgccgccg ttagcttcag ccgggaagaa tttgaactct ttcaaaccgt agtccatacc
    10801 cagcatcagt tcggaaacag tgctgatccc cggaatcaga ggaatagtcc cttcggtagc
    10861 agctttcagc agcggctcgg tcagacccgg gctaattgcg aactgtgcac ccgcttcagt
    10921 gacttctgcc agctgctgtg gattcagcac cgtaccggca cccacaatcg cttcaggcac
    10981 ttctttggcg atagcacgga tagcgtcaac tgcacactcg gtacgcagag tcacttccag
    11041 aacgcgcacc ccaccagcaa ccaacgcttt tgccatcggc accgcgtgtt ccagtttttt
    11101 taccacgata accggtacaa ccgggccggt ggtcaggatt gattctgcac ttgttttcca
    11161 gtttttcatc agagttttct ctcgcctgat tacaaatttg tcgtcttaaa aagtgataca
    11221 ggttgcgccc tgttcggcac cggacagttt ttcacgcaag gcgctgaata attcacgtcc
    11281 tgttctcacg cgtgacgcgc tcaggtcagg aatgtgcggt tcgcgagcag ccagttccgc
    11341 ttcgtctacc agcagtgtca gttcgcctgt ctgtccattc acacgaatga tgtccccgtc
    11401 gcgcactttt gccagcagcc cgccatcgta ggcttctggt gttacgtgga tagctgacgg
    11461 cactttacct gaagcgccgg agagtcgtcc atcggtaact aacgcaattt tgaaacaccg
    11521 gtccaataat acaccaagtg gcggcatgag tttatgtaat tctggcattc cgttcgcttt
    11581 tggcccctga tgacggacaa caacgacaca atcgcggtcc agcaaacccg cttcaaaggc
    11641 cggcataacg tcatgctggc tttcaaaaac aaccgctggc gcttcaatca cctggttctc
    11701 aaccggcacg gcagaggttt tcataaccgc acggcccagg ttaccgctta acacttttgt
    11761 cccaccatga tgagagaaag gttgttcgaa ggaagcgatc acattgctgt cgagtgattt
    11821 ttccgcccct tcccgccagt ccagttcacc attattcagc catggttcaa gggtataacg
    11881 aaacagacca aagcctgcca ccgtattgac atcttcatgc agcaggcctg ctttgagcag
    11941 ttcacgcacc agaaccggta cgccacctgc cgcctggaag tggttaatat cggccggacc
    12001 gttcgggtag agacgtgcca tcagcggtac aacatcagaa aggtcagaga agtcatccca
    12061 gttaatctga ataccggccg cgcgcgccat cgccaccagg tgcatggtgt ggttagtgga
    12121 accaccggtc gccaacagtg caacgatacc gttcaccacc actttctcat cgatcatctt
    12181 accgatcggc atccattcat taccattacc ggtcatgcgt gtaacctgac gcgcagcggc
    12241 agcggtcaaa gcatcgcgca gcggagaatc cggatgaaca aaagaagagc ctggcaactg
    12301 catccccata aattccacca ccatctggtt ggtgttggca gtaccgtaga aagtacatgt
    12361 tcccggcgca tggtaagacg cggcttctga ctccagtaag gccatgcggt ccactttacc
    12421 ttcggcataa agctggcgaa tacgcacttt ttctttattt ggcaaaccgc ttgccatcgg
    12481 tccagacggc acaaacaccg caggcaaatg accaaacgac agggctgcca tcgtcagacc
    12541 cgggacaatc ttgtcgcaca caccgaggaa cagagcacca tcaaacatgt tatgggacag
    12601 ccccaccgcc gcagacatcg ctatcacttc gcggcttagc agcgacaatt ccattccatc
    12661 ctgcccctgg gtgacaccat cacacatcgc cggaacaccg cccgcaacct gaccaaccgc
    12721 attcgcttca tgcagggctt tacgaatgat ttctggatag tgttcataag gctggtgcgc
    12781 ggagagcatg tcgttatagg aggtgatgat ggcgatattg ttacgcaaca tgcttttcaa
    12841 agaggctttg tcttctggct ggcaggcagc gaaaccgtgt gccaggttac cgcatgccaa
    12901 ctgcgaacga tgaacggtcg acgttttcgc ttgttctatc cgggcgagat aagcagagcg
    12961 agtctcgcgc gaacgttcaa tgattcgatt tgttacgcgt aacaattgtg gattcataaa
    13021 ggctcctgaa attgagttgt cagagcagga tgattcacaa cgcgtttcat tcagaggatt
    13081 tatgagtgaa acgcctgtaa ccggagctca tagggcaaaa acgtttcagt cagtgtaata
    13141 aaaaaagcct cgtgggtgaa tccgcacgag gcctgaaagt gtaaaaattg ttctacaatc
    13201 tgtgcaagat tatgttaccg gtaaaataac cataaaggat aagcgctgat attactcaaa
    13261 ctcattccag gaacgaccat cacgggtaat catcgccacc gaggcaacgg gtccccaggt
    13321 tccggcctga tacggtttcg gcgcatcatt gtccatcgcc cacgcctcag taatggagtc
    13381 tacccatttc caggcttctt ccacttcgtc gcgacgtaca aacagtgcct gaataccacg
    13441 catggtttcc agcagcaaac gttcataggc atccgccaga tgcgtctgat taaaggtttc
    13501 tgaatagctc agatccagct tggtgatttg caggttatgt ttgtggtcaa ggccaggaac
    13561 tttattcagt acctggatat ccacgccttc atcaggttgc agacggatag tcagtttatt
    13621 ctgcggcaga tcctgccacg actctttaaa cagattcagt tcaggtgttt tgaaatagac
    13681 cacgacttca gaacatttgg tcggcagacg tttaccagta cgcaggtaga atggcacacc
    13741 ggcccagcgc cagttatcaa tgtcgacgcg gatcgccacg aaggtttctg tattgctact
    13801 tttgttcgcg ccctcttctt ccagatatcc cggcactttt ttgccctggg cgaagcccgc
    13861 agtatattgc ccgcgtacgg ttttttcgcg tacgttggag cggtcgatgc ggcgcaggga
    13921 cttcagtact ttcacttttt catcgcggat gctgtctgcg ctcaggtcag atggcggaga
    13981 catcgcaatc atgcagagaa tttgtagcag gtggttctga atcatgtcac gcatctgacc
    14041 ggctttatca aaatagcccc agcgcccttc gatccccact tcttctgcca cggtaatctc
    14101 aacatgatca atggtgcgat tgtcccagtt attcacaaac agggagttag caaaacgcag
    14161 cgccaacagg ttcagcaccg tttctttacc aagatagtgg tcgatacggt aaacctggca
    14221 ctcctcgaag tattcgccaa cctgatcatt gatttcctgc gaggtcgcca gcgacgtccc
    14281 cagcggtttc tccatgacta cgcgtgccgg tttagcattc agttttgcct cgccaagccc
    14341 tttgcaaatt gcgccaaaag tgctgggcgg catggcaaag tagttaatag tgatacgatt
    14401 tttttgatcc agcatcgcgc cgagacggtt gaatgcagca gtgtcattga catcgagatt
    14461 acaaaaatcc agacgtgcac tcagggtgtc ccataaacct tcatcaatgg tttctttcat
    14521 gaaagtttcg agcgcctcgc ggacaacttt ggtatatgcc gctttatccc agtcagcacg
    14581 ccctacgccg ataatccggg tgtccgggtt gagctgaccg gctttttcca gttgatacag
    14641 ggaaggcagc aatttacgac gcgcaaggtc gcctttcgcg ccgaaaatga ccaggtcaca
    14701 ggcctgggct gtttgcgtta ccgccatgtc attctcctta agttaactaa cccggtactt
    14761 aagccagggt atacttgtaa ttttcttacg gtgcactgta ctgcttttcc gagcttgcga
    14821 aaactgtaaa cgcttatcca cccgtgcgat tacgggaaaa gcgcgcaaag tgcggcaaaa
    14881 ctgataaaaa aatcatcgtt ttccattcgg taaaaatctg acactgatca tgttatgaaa
    14941 aaaaataaca acttttttat ctgcttttgt cattaacacg gcacacaggc gtaatatctg
    15001 acaaaactgc atttcgattt ctttcagtgc ggaaatcgtc attacccgtg agtctcttta
    15061 catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca aatcagagcg
    15121 caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga gtattgctgc
    15181 tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc gcagcatgga
    15241 cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga atggcactcc
    15301 ctatgttaat cgcaacgtca atgaagatga cagcgttgaa tcatacacgg ggaaaatatt
    15361 tgagtccgca atggcaacgc tcgatcatgt ccgtcattca ctggataaat ctgccatcaa
    15421 ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat taggctcttc
    15481 tgccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc cggtggtgta
    15541 ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg gagacgtggt
    15601 ggtgctgatt tctcacacag gaagaacaaa aaatctggtc gagctggcgc agctggcacg
    15661 cgaaaacgac gccatggtga ttgccctcac ctctgcgggt accccgctcg cccgggaagc
    15721 aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc ccatggtttc
    15781 tcgacttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt tgcgacgcgg
    15841 tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat cgcgttttga
    15901 taagcagtta cttaatttaa gtgacgatcg ctaaaagcga ctgtcactgt cctaatctta
    15961 tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt cggatttcat
    16021 gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaaa aggcttcgca
    16081 gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat cttgaaaaag
    16141 ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg cctgaagatc
    16201 acaaaatgca cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt catgtggcta
    16261 ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa ggcaaagttt
    16321 tcctcaatat tggggataaa ttcctgctcg acgccaacct aggtaaaggt gaaggcgaca
    16381 aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgtcgtgcct ggtgacatcc
    16441 tgctgctgga cgatggtcgc gtccagttaa aagtactgga agttcagggc atgaaagtgt
    16501 tcaccgaagt caccgtcggt ggtcccctct ccaacaataa aggtatcaac aaacttggcg
    16561 gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag actgcggcgt
    16621 tgattggcgt agattacctg gctgtctcct tcccacgctg tggcgaagat ctgaactatg
    16681 cccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag gttgaacgtg
    16741 cggaagccgt ttgcagccag gatgcaatgg atgacatcat cctcgcctct gacgtggtaa
    16801 tggttgcacg tggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc ggcattcaga
    16861 aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt aatcacggcg acccagatga
    16921 tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac gtagcaaacg
    16981 ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgct gggcagtatc
    17041 cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa atcccgagca
    17101 tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa gctattgcca
    17161 tgtcggcaat gtacgcagct aaccacctga agggcgttac ggcgatcatt accatgaccg
    17221 aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca attttcgcca
    17281 tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt acgccggtgc
    17341 actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat ctgctgcgcg
    17401 ataaaggtta cttgatgtct ggtgacctag tgattgtcac ccagggcgac gtgatgagta
    17461 ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaagtacgt tgccggatgc
    17521 ggcgaaaacg ccacatccgg cctatagttc aattatagtt caacagattt cgaatattct
    17581 gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc ttttttattt
    17641 gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc gagttttcag
    17701 caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct cgacttcttc
    17761 attcatccgc cgctcaatcg tatgatcatc cgcctctaac agatcatcca tcggtgggcg
    17821 cacctgaatc gtcagacgat gcgtcttgcc atcataaatc ggaaacagcg gtacaacgcg
    17881 cgcacggcat actttcatca aacgaccaat cgcgggcaac gtcgctttat aggtggcaaa
    17941 gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat aatatcccca
    18001 gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg catgcagacg
    18061 accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt tgccctgatt
    18121 atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag gaatatcgac
    18181 ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgccgca tctcttcgat
    18241 gatctccagc ccttgccagc caacgcgcgg ctgaattttc tccggcccgc gtattgccaa
    18301 ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa caatcgcttc
    18361 gcgttcagct tcactacgtt ctggaaagca gagcgacaga ttgattaacg cacgacggcg
    18421 tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg gatcacggaa
    18481 ctttggcggc gttaaagcga tacccgccat cgctgctaca cccagccatg ctccccagta
    18541 gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat tttttttcgt
    18601 ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt gtagcggcgc
    18661 aacttgcccc gcaccaaata aaaaaagccg gtactgactg cgtaccggct gcgaatggat
    18721 gttaattaat caaacagtag ctgcggcaca atctctttgg cctgcgccag gaattcgcga
    18781 cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg gtttacggcc
    18841 tgctggttta tccatacttc atagtgcaga tgcggcccag ttgaacgtcc ggtattaccg
    18901 gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag aatcttgcgc
    18961 aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata ataacctgct
    19021 gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac tggcgtacct
    19081 tgtggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gacctgtcac cggattagtt
    19141 cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa tcgcaagaat
    19201 cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcctcagc gcggattgcg
    19261 taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg ctcacgttta
    19321 ccatcaagca tttctcgaga cattaatacc gcaaattcat cgcctttttt cagtttgcgg
    19381 aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct ggttaaaccg
    19441 gcgtttctgg cgctggcaac aaagctccct ccgacggtac ctttcagcag attgttaacc
    19501 cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt acggtcatag
    19561 gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc gtccgcggtt
    19621 aatgtccagg agagttgttg accgattttc aggttacgca attctttgtc ggcagcagcg
    19681 agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct tagcgtatcg
    19741 ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc atccagttcg
    19801 tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact ggcttcaggt
    19861 aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg cgtggcatca
    19921 cggtgataaa catagggccg ccagacagcg acggccagag taagaacggt gagcgacccc
    19981 aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga gcgggctatc
    20041 tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata ctggttggct
    20101 aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt ccccagggga
    20161 tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac cgctggcctg
    20221 aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt tcttatttca
    20281 tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc aagcggtgtc
    20341 agtccgaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata acctttcccc
    20401 ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggccaattg tgcctcaaaa
    20461 tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac taattttcca
    20521 tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg catgttgaaa
    20581 tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc atcatcgccg
    20641 tgaatacttt tcatcagtag cggtttcaca tcttcaagct gcgcaatcgt tacctgcttc
    20701 gcttctggta atttgcttac cggtttttgc ataaacgctt ccatctccgg gccaacccaa
    20761 acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga ataatcatgt
    20821 tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc aatggcagaa
    20881 gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc ctgtgttgca
    20941 cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg taacataatg
    21001 cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat gactactgca
    21061 agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt tcttttggcc
    21121 aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt ttgactttac
    21181 ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg ctggtaacac
    21241 ccgatgaagg ggttatcaag cgcaacggaa aactgcgcat cggctatgta ccgcagaagc
    21301 tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta cgccctggta
    21361 cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac ctgattaacg
    21421 caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actattagcg cgagcattgt
    21481 taaattgccc gcaattatta gtgctggatg aacccactca aggcgtggat gtaaatggcc
    21541 aagtggcgtt atatgacctt attgaccagt tgcgtcgtga actggattgt ggcgttttaa
    21601 tggtttctca cgatctgcat ctggtgatgg caaaaaccga tgaagtgctg tgcctgaatc
    21661 accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag tttatttcaa
    21721 tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat aatcatcgtc
    21781 acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga ttgaattatt
    21841 atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc tgggttcgtt
    21901 tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct cattacttgg
    21961 tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg ccgttacgct
    22021 gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga tcgacacgtt
    22081 attagggatt atggcgcaca gtgccctgtc gctgggccta gtggtcgtta gtctgatgtc
    22141 taacattcgt gttgatttga tggcttacct gttcggtgat ttactggcag tgacgccaga
    22201 agatctcatc tctattgcga ttggcgtggt catcgtggtg gctattttgt tctggcaatg
    22261 gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg gtgtgaaatt
    22321 acagcaagtg aaattgttgt tgatgctggt gacggcattg acgattggtg tagcgatgaa
    22381 atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta ctgcgcgtcg
    22441 ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga tggtggcagt
    22501 aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt cggtggtgct
    22561 atgtgcggca ctgttattta ttatcagtat gatgaaaaag caggccagct aatctatcgc
    22621 tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc gctatctctg
    22681 gctggcctga taagacgcga acagtgtcgc atcagacaca ctgccagtgt cggatgcggc
    22741 gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt gattccacgc
    22801 ccgcgtcgtc gccatacgcc cgcgcggtgt acgctgcaaa aagccttgct gaatcaaata
    22861 aggttccagt acatcttcaa tggtttcacg ttcttcgcca atggctgccg ccaggttatc
    22921 cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaacaatt tgcggtccat
    22981 ataatcgaaa ccttcagcat cgacattcaa catatccagc gcctgagcag cgatatctgc
    23041 cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca gcagacggtt
    23101 ggcaatacgt ggcgtaccgc gagcgcgacg agctacttcc tgcgcaccgt catcactcat
    23161 ctcaagcccc ataaagcgtg cactgcgact gacgatatat tgcagatccg gcacctgata
    23221 aaactccaga cgttgcacaa taccaaaacg gtcgcgcaac ggtgatgtca gcgaacctgc
    23281 gcgcgtggtt gcgccaatca gggtaaacgg cggcaaatca attttaatgg agcgtgccgc
    23341 cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccgggt acagcacttc
    23401 ttcaacaact ggcgataaac ggtggatctc atcaataaac agcacgtcat gcggttcaag
    23461 gttagtgagc atcgcagcca aatcgcccgc cttttccagc accggaccag aagtcgtgcg
    23521 taaattaacg cccatttcat tggcgacaat gttggcaagc gtagttttac ccaaccccgg
    23581 aggaccaaaa atcaacaaat gatcgagggc atcgccgcgc agtttcgctg ctttgatgaa
    23641 aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta atttagggcg
    23701 aatggcgcga tctgctacat cttccggcaa agtggtaccg gcagaaatca gacggtctgc
    23761 ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa tgtttcactg
    23821 ctggcgtcag ggcgagcgat tttgctcacc atgcggcttg cttcttgtgg tttatagcct
    23881 agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg gctggcagga
    23941 gacgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc tttaaatcgg
    24001 tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg cagtttcacc
    24061 agtgccccca cttcttcacg ctcaacggca ttaacgaact gctgcgctga cattccggag
    24121 aggatcgcca gggccaactt cgggccgacg ccgttggttt tgatcaactc tttgaacaac
    24181 gtgcgttctt gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg caccacaaag
    24241 tgggtgaaaa cgatcgcttc ctgacccgct tcagggagtt cataaaaaca ggtcatcggc
    24301 atatgcactt catagcctac gccgcccact tcaattaaca ccagcggggg ttgtttttca
    24361 atgatgatgc ctctgagtct gcctatcaca tgacgctcct gcgtaatgaa tcaaagataa
    24421 tgctgtatga taaaaaaatg ctggatagat atccagcgta ggatgaagaa aacttgcgag
    24481 gtgtcc
//
LOCUS       DAJTNM010000049        24463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTNM010000049 DAJTNM010000000
VERSION     DAJTNM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.049"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..487)
                     /locus_tag="OO172_002113"
     CDS             complement(<1..487)
                     /locus_tag="OO172_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5512301.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LL"
     gene            760..948
                     /locus_tag="OO172_002114"
     CDS             760..948
                     /locus_tag="OO172_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5512302.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            960..1670
                     /gene="bcsQ"
                     /locus_tag="OO172_002115"
     CDS             960..1670
                     /gene="bcsQ"
                     /locus_tag="OO172_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5512303.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1667..4280
                     /gene="bcsA"
                     /locus_tag="OO172_002116"
                     /pseudo
     CDS             1667..4280
                     /gene="bcsA"
                     /locus_tag="OO172_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4291..6630
                     /gene="bcsB"
                     /locus_tag="OO172_002117"
     CDS             4291..6630
                     /gene="bcsB"
                     /locus_tag="OO172_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5512304.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6637..7743
                     /gene="bcsZ"
                     /locus_tag="OO172_002118"
     CDS             6637..7743
                     /gene="bcsZ"
                     /locus_tag="OO172_002118"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5512305.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7802..7978
                     /locus_tag="OO172_002119"
                     /pseudo
     CDS             7802..7978
                     /locus_tag="OO172_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8306..10054
                     /gene="bcsC"
                     /locus_tag="OO172_002120"
                     /pseudo
     CDS             8306..10054
                     /gene="bcsC"
                     /locus_tag="OO172_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10136..12124
                     /gene="hmsP"
                     /locus_tag="OO172_002121"
     CDS             10136..12124
                     /gene="hmsP"
                     /locus_tag="OO172_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5512306.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12308..13594
                     /gene="dctA"
                     /locus_tag="OO172_002122"
     CDS             12308..13594
                     /gene="dctA"
                     /locus_tag="OO172_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5512307.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFSSGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13815..15311
                     /locus_tag="OO172_002123"
     CDS             13815..15311
                     /locus_tag="OO172_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5512308.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15407..16336)
                     /gene="kdgK"
                     /locus_tag="OO172_002124"
     CDS             complement(15407..16336)
                     /gene="kdgK"
                     /locus_tag="OO172_002124"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5512309.1"
                     /translation="MSKKIAVIGECMIELSEKDADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16568..17335
                     /gene="pdeH"
                     /locus_tag="OO172_002125"
     CDS             16568..17335
                     /gene="pdeH"
                     /locus_tag="OO172_002125"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5512310.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17405..19465
                     /locus_tag="OO172_002126"
     CDS             17405..19465
                     /locus_tag="OO172_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5512311.1"
                     /translation="MSKAGKITAAISGVFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19647..20969)
                     /locus_tag="OO172_002127"
     CDS             complement(19647..20969)
                     /locus_tag="OO172_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5512312.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21380..22393)
                     /gene="yhjD"
                     /locus_tag="OO172_002128"
     CDS             complement(21380..22393)
                     /gene="yhjD"
                     /locus_tag="OO172_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5512313.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22442..23341)
                     /locus_tag="OO172_002129"
     CDS             complement(22442..23341)
                     /locus_tag="OO172_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5512314.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23861..24463
                     /locus_tag="OO172_002130"
     CDS             23861..24463
                     /locus_tag="OO172_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5512315.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5788 a         6159 c         6734 g         5782 t
ORIGIN      
        1 caagcaacaa tgaaaattgt ttatcgttat tatttccggg attaattacc aaaagcgaac
       61 aatggtttaa cctgctccat ttattcattt tatccaacca tgagcgaagc cgctcggcag
      121 gaatgttttg ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta tggggatcaa
      181 tagaacactg caaatcacgg gccaaatagt atagaccttt ttcatgattt agcattgaaa
      241 ataattttat tttttccggc ccttgagaat catctaattg aaagattttc gccggatcgc
      301 tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt gtttgattcg
      361 ccagactgat agcatcttca tggcgatcga cgttaaacca ccagacgccg cctgctggca
      421 tatgtcgcag ctcatcccat aatgatgaga taccgataga gaatacaggg tccacaatgt
      481 ccctcatgtc accattttgt ctgattatca gtttactagc gaaagctaag aaataaacct
      541 accattgaaa ttaaaactta tcagatttag catgtgaatc aatttagaag gtcacaagac
      601 gtgtgatttt agtcttttcg tttttaactt tttctgttgc gtaacggaaa gtcaaaaagt
      661 gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg ttagaattgt
      721 atcatgaatg gtacggttat ttcataggga tcaagcaaaa tgaataacaa tgaaccagat
      781 actctgcctg atcccgcgat aggctatatc ttccagaatg atattgtggc gttaaagcag
      841 gcattttcac tgcctgatat tgattatgcc gatatttccc aacgcgaaca gttggccgcg
      901 gcattaaaac gctggccgtt gctggcagag tttgcgcaac aaaagtaggg gattggtgaa
      961 tggccgtact gggattgcag ggggtgcggg gaggcgtggg gacaacaacc atcaccgccg
     1021 cattagcctg gtcattacaa atgttgggag aaaatgtcct agtggtcgat gcctgcccgg
     1081 acaacttgtt gcgcctgtca tttaatgttg attttaccca ccgtcagggc tgggccagag
     1141 cgatgctgga tggccaggac tggcgtgacg ctgggttgcg ctacacctcg cagctcgatt
     1201 tgctgccttt tggtcagtta tccattgaag aacaagaaaa tccacagcac tggcaaaccc
     1261 ggctgagcga tatttgctcc ggcttacagc aactaaaagc cagcgggcgt taccagtgga
     1321 ttttaatcga cttaccgcgt gatgcctcgc agataaccca ccagctgctg agtttgtgcg
     1381 agcactcgct ggcaatcgtc aatgtggatg ccaactgcca tatccgactg catcagcaag
     1441 cgctgccgga tggcgcacat attttgatta atgacttccg tattggcagt caggttcagg
     1501 acgatattta ccagctttgg ttgcaaagcc agcgccgatt actgccgatg ctcattcatc
     1561 gtgatgaagc gatggctgaa tgcctggcgg ctgaagagat actgacgctg gcgaactggt
     1621 gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac
     1681 ccggtggttg cttatcccgc cggtcaacgc gcggcttatc gggcgttatc gcgattatcg
     1741 tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt ttctggatga tcctggcctg
     1801 gatttttatt ccgctggagc acccgcgctg gcaacgtatt cgcgcagaac ataaaaacct
     1861 gtatacgcca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     1921 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     1981 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     2041 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     2101 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     2161 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     2221 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     2281 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     2341 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     2401 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     2461 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     2521 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     2581 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     2641 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     2701 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     2761 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     2821 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     2881 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     2941 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     3001 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     3061 gtggctatac ctccgtgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     3121 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtacaa atcttccgtc
     3181 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     3241 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     3301 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     3361 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     3421 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     3481 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     3541 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     3601 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     3661 ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg
     3721 cggtatcggt agaaagcaaa caggtacgcc gatcgcaccg cgtggagatg acgatgcccg
     3781 cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     3841 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     3901 tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg
     3961 gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc
     4021 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     4081 agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     4141 cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     4201 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     4261 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca
     4321 gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     4381 ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg
     4441 caagtgatgt caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg
     4501 cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt
     4561 ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     4621 gcgatgctca acctcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta
     4681 aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt
     4741 aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg
     4801 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     4861 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     4921 aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc
     4981 ttgccgatgg tctttgcggg tgcgccggat gttgggctgc aacaagcatc tgccattgtc
     5041 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     5101 caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg
     5161 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     5221 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa
     5281 ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa
     5341 ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc
     5401 acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca
     5461 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     5521 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     5581 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     5641 cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct
     5701 attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac
     5761 ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg
     5821 gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat tccgatgccg
     5881 gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa
     5941 accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact
     6001 gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat
     6061 ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa
     6121 ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca
     6181 ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa
     6241 acccagtcaa cgctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg
     6301 tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg
     6361 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
     6421 atccgcgagt acggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg
     6481 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
     6541 gcggctatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt
     6601 cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggaatc
     6661 gtgacgatgc tgttgttggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg
     6721 gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg
     6781 cgcaaaatca ccacctccga agggcaaagt tacggtatgt tctttgccct ggcggctaac
     6841 gaccgtgcag ctttcgataa tattctcgac tggacgcaga acaatctcgc tcagggttct
     6901 ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg
     6961 ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg
     7021 gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcgttgct aaaacgtatc
     7081 gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg
     7141 ggttttgctg aggataacag ctggcgtttt aaccccagct acatgccgcc gacgctggcg
     7201 cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta
     7261 ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa
     7321 ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac
     7381 atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt
     7441 aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct
     7501 acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcctttttta
     7561 caaaaccgcg atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt tcccggcagc
     7621 gatacctatt acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc
     7681 cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac
     7741 taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa
     7801 ccatcagcag accctgcgga tggacagcgg caacactaat gccgtgcgcg ggctggcgaa
     7861 tatttaccgc cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
     7921 ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
     7981 ggcagaggta ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg
     8041 actggcgctg gaccccggca gcgtatggat tacttaccga ctttcgcagg atctctggca
     8101 ggccggacaa cgcagccagg ccgatacgtt aatgcgcaat ctggcgcagc agaagccgaa
     8161 caacccggag caggtttacg cttacgggct gtacctctca ggtcacaacc aggacagagc
     8221 ggcgctggcg catatcaaca gcctgccgcg cgcgcagtgg aacagcaata ttcaggagct
     8281 ggttaatcga ctgcaaagcg atcaggtgct ggaaaccgct aatcgcctgc gagaaagcgg
     8341 caaagaggct gaagcggaag cgatgctgcg tcagcaacca ccttccacgc gtattgacct
     8401 cacgctggct gactgggcgt aacaacgacg tgattacacc gccgcccgca ctgcatatca
     8461 gaatgtcctg acgcgggagc caactaacgc cgatgccatt cttggtctga cggaagtgga
     8521 tattgctgcc ggtgacaaag cggcggcacg tagccagctg gcgaaactgc ctgccaccga
     8581 taacacctcg ctgaacactc agcggcgcgt ggcgctggca caggcgcagc ttggagatac
     8641 cgcagcggcg cagcagacgt ttaataagtt gatcccgcag gcaaaatctc agccaccgtc
     8701 gatggaaagc gcgatggtgc tgcgcgacgg tgcgaagttt gaagcgcagg cgggcgatcc
     8761 aaagcaggcg ctggaaacct acaaagacgc catggtcgca tccggtgtga ctacgacgcg
     8821 tccgcaggat aacgacacct ttacccgact gacccgtaac gacgagaaag atgactggct
     8881 gaaacgcggc gtgcgcagcg atgcggcgga cctctatcgc cagcaggatc ttaacgtcac
     8941 ccttgagcac gattactggg gttcgagcgg caccggtggt tactccgatc tgaaagcgca
     9001 cactaccatg ttgcaggtgg atgcgccgta ttctgacggg cggatgttct ttcgcagtga
     9061 tttcgtcaat atgaacgtcg gcagtttctc cactaatgcc gatggcaaat gggatgacaa
     9121 ctggggcacc tgtacattac aggactgtag cggcaaccgc agccagtcgg attccggtgc
     9181 cagcgtggcg gtcggctggc gaaatgacgt ttggagctgg gatatcggta ccacgccgat
     9241 gggcttcaac gtggtggatg tggtcggcgg catcagttac agcgatgata tcgggccgct
     9301 gggttacacc gttaacgccc accgtcggcc catctccagt tctttgctgg cctttggtgg
     9361 gcaaaaagac tccccgagca ataccgggaa aaaatggggt ggcgtacgtg ccgacggtgt
     9421 ggggctaagt ctgagctacg ataaaggtga agtaaacggc gtctgggcat cgcttagtgg
     9481 cgaccagtta accggtaaaa atgtcgaaga taactggcgc gtgcgctgga tgacgggcta
     9541 ttactataag gtcattaacc agaacaatcg ccgcgtcaca atcggcctga acaacatgat
     9601 ctggcattac gacaaagatc tgagtggcta ctcactcggt cagggcggtt actacagtcc
     9661 gcaggaatac ctgtcgtttg ccataccggt gatgtggcgg gagcgcacgg aaaactggtc
     9721 gtgggagctg ggggcgtctg gctcgtggtc gcattcacgc accaaaacca tgccgcgtta
     9781 tccgctgatg aatctgatcc cgaccgactg gcaggaagaa gctgcgcggc aatccaacga
     9841 tggcggcagc agtcagggct tcggctacac ggcgcgggca ttacttgaac gacgtgttac
     9901 ttccaactgg tttgttggca cggcaattga tatccagcag gcgaaagatt acgcacccag
     9961 ccatttcctg ctctacgtac gttattccgc cgccggatgg cagggtgaca tggatttacc
    10021 gccgcagccg ctgatacctt acgccgactg gtaagttttc agatagcgcc tctcttaatg
    10081 ccgctgcgat cgggtatact cgggcggcaa tctgggattt ccggggggag acaatttgcg
    10141 cgtaagtcgc tcgttaacaa tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt
    10201 cgtttttatt ttttgcaccg ttttgctgtt ccatctggtc cagcagaatc gctataacac
    10261 ggctacgcaa ctggaaagca ttgctcgctc tgtccgcgaa cccttatctt cagctatttt
    10321 gaaaggcgat attcccgaag cggaagctat tcttgccagc attaaaccgg caggcgtggt
    10381 cagccgtgcc gatgtagtgc tgcctaacca gttccaggcg ctgcgtaaaa gttttattcc
    10441 agagcgtccg gtgccggtaa tggttactcg cctgtttgag ctaccggttc aaatctcgct
    10501 gggcgtctac tcgctcgaac gtccggcaaa tccgcagcca atagcctatc tggtgctaca
    10561 ggcggattcc ttccgtatgt ataagttcgt gatgagcact ctctcaacgt tagtgaccat
    10621 ttacttactt ttgtcgctta tcctgaccgt cgccatcagc tggtgcatta accgcctgat
    10681 tttgcatccg ttacgcaata ttgctcgcga acttaacgcc atcccagccc aggagcttgt
    10741 tggtcaccaa ctggcattac cgcgtctgca tcaggacgat gaaatcggta tgttggtgcg
    10801 cagttacaac ctcaaccagc aattgctgca gcgccattat gaagaacaga acgaaaatgc
    10861 gatgcgcttc ccggtgtcgg atttgccgaa caaagccttg ctgatggaga tgctggagca
    10921 ggttgtcgcg cgtaaacaaa ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga
    10981 tactgcgggc gtgctgaaag aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa
    11041 actcaaatcg gtactgtcgc cacgtatgat cctcgcgcag attagcggtt atgactttgc
    11101 tgtcattgcc aacggtgtac aggaaccgtg gcacgcaatc actttgggtc agcaagtact
    11161 cactatcatg agcgagcgcc tgccgattga acgtattcaa ctccgtccgc actgtagcat
    11221 tggcgtggcg atgttctacg gcgatctcac cgccgaacag ctttacagtc gcgctatttc
    11281 tgcggcattt accgctcgcc ataaaggcaa gaatcagatt cagttctttg atccgcagca
    11341 gatggaagca gcccagaagc ggttgacgga agagagcgat atccttaatg cactggaaaa
    11401 tcatcagttt gcaatttggt tacagccaca ggtcgagatg accagcggta aactggtcag
    11461 tgcggaagtg ttactgcgta tccagcaacc ggatggcagt tgggacctgc cggatggctt
    11521 aatcgatcgc attgagtgct gtgggctgat ggttaccgtc ggtcactggg tgctggaaga
    11581 gtcctgtcga ttgcttgcag cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa
    11641 cctctctgcg ctgcaactga tgcacccgaa tatggtggcg gatatgctgg aactgttaac
    11701 ccgctatcgc attcagccgg gaacactgat tctggaagtg acagaaagcc gacgtattga
    11761 cgaccctcat gctgcggtgg caatcctccg tccgctgcgc aatgccggag ttcgggtggc
    11821 gctggatgat ttcggcatgg gctacgcagg gctgcgtcag ctgcagcata tgaaatcgtt
    11881 gccaatcgac gtactgaaaa tcgacaaaat gtttgttgaa ggcttgccgg aagatagcag
    11941 catgattgct gcaattatca tgctggcgca gagcctgaac ttacaaatga ttgccgaagg
    12001 cgtggagact gaagcacaac gagactggct ggcaaaagcg ggcgttggta ttgcccaggg
    12061 cttccttttt gctcgcccac tccctattga aatcttcgaa gagagttacc tggaagaaaa
    12121 gtagctaccc caaactgatt acaaaacttt aaaaagtgct ggtttgtgcg agccagctca
    12181 aactttttaa cctttttgtt tcaattatga tccaggtaca tttctgtgat gttgtctggg
    12241 tgttatttta aggccgcagg tacccccata accttacaag acctgtggtt ttactaaagg
    12301 acaccctatg aaaacctctc tgtttaaaag cctttacttt caggtcctga cagcgatagc
    12361 cattggtatt ctccttggcc atttctatcc tgaaataggc gagcaaatga aaccgcttgg
    12421 cgacggcttc gttaagctca ttaagatgat catcgctcct gtcatctttt gtaccgtcgt
    12481 aacgggcatt gcgggcatgg aaagcatgaa ggcggtcggt cgtaccggcg cagtcgcact
    12541 gctttacttt gaaattgtca gtaccatcgc gctgattatt ggtcttatca tcgttaacgt
    12601 cgtgcagcct ggtgccggaa tgaacgtcga tccggcaacg cttgatgcga aagcggtagc
    12661 ggtttacgcc gatcaggcga aagaccaggg cattgtcgcc ttcattatgg atgtcatccc
    12721 ggcgagcgtc attggcgcat tttccagcgg taacattctg caggtgctgc tgtttgccgt
    12781 actgtttggt tttgcgctcc accgtctggg cagcaaaggc caactgattt ttaacgtgat
    12841 cgaaagtttc tcgcaggtca tcttcggcat catcaatatg atcatgcgtc tggcacctat
    12901 tggtgcgttc ggggcaatgg cgtttaccat cggtaaatac ggcgtcggca cactggtgca
    12961 actggggcag ctgattatct gtttctacat tacctgtatc ctgtttgtgg tgctggtatt
    13021 gggttcaatc gctaaagcga ctggtttcag tatcttcaaa tttatccgct acatccgtga
    13081 agaactgctg attgtactgg ggacttcatc ttccgagtcg gcgctgccgc gtatgctcga
    13141 caagatggag aaactcggct gccgtaaatc ggtggtgggg ctggtcatcc cgacaggcta
    13201 ctcgtttaac cttgatggca catcgatata cctgacaatg gcggcggtgt ttatcgccca
    13261 ggccactaac agtcagatgg atatcgtcca ccaaatcacg ctgttaatcg tgttgctgct
    13321 ttcttctaaa ggggcggcag gggtaacggg tagtggcttt atcgtgctgg cggcgacgct
    13381 ttctgcggtg ggccatttgc cggtagcggg tctggcgctg atcctcggta tcgaccgctt
    13441 tatgtcagaa gctcgtgcgc taactaacct ggtcggtaac ggcgtagcga ccattgtcgt
    13501 tgctaagtgg gtgaaagaac tggaccacaa aaaactggac gatgtgctga ataatcgtgc
    13561 gccggatggc aaaacgcacg aattatcctc ttaatctcac cacttatgcc cgcactccct
    13621 tccccgagtg cgggtgtttg cgcataattg acccctgtgc ctgaacttac cacctgggtg
    13681 agttcaggtt tatttcactt cttccgtgac attttcatgt tcttgcggtc taacacgaag
    13741 tgtttttaac gtcatttaga ctgaccgaca acgggcagtt tttttattac caggatagtt
    13801 gatcaggggt tcacatgcag ggcataaaaa ttcgactttt agcgggcggt ttgctgatga
    13861 tggccactgc tggctatgtg caggcagatg cgctccagcc tgatccagca tggcaacagg
    13921 ggacgctttc caacggttta cagtggcaag tgctgaccac cccccagcgt cccagcgatc
    13981 gtgttgaaat tcgcctgctg gttaataccg gttcgctcgc cgaaagtaca caacagagcg
    14041 gttacagtca cgccatccct cgtattgcgc taacgcaaag cggtggcctt gacgcagcac
    14101 aggcgcgttc attgtggcag caggggatcg accctaaacg cccgatgccg ccggtaattg
    14161 tctcttatga caccacgctg tttaatctga gtttgcccaa taaccgtaac gatttgctga
    14221 aagaagcgct ctcttatctg gcaaatgcca ctggcaaatt gaccatcaca ccagaaacca
    14281 tcaaccacgc gctgcaaagt caggacatgg tggcaacctg gcctgccgat actaaagagg
    14341 gctggtggcg ctatcgtctg aaagggtcaa ccttgttagg tcacgatcct gccgatccgc
    14401 tgaaacaacc cgttgaagcg gaaaaaatta aagatttcta tcagaaatgg tacaccccgg
    14461 atgcaatgac gctactggtg gtgggaaacg tggatgcgcg ctcggttgtc gaccaaatca
    14521 acaaaacgtt tggcgaactg aaaggcaaac gtgaaacgcc agctccggtg ccgacgcttt
    14581 ctccgctgcg tgcggaagcg gtgagtatta tgactgacgc ggtgcgtcag gaccggttat
    14641 ctatcatgtg ggatacgccg tggcagccga ttcgtgaatc agccgcactg ctgcgctact
    14701 ggcgtgcgga cctggcccgc gaggcgctgt tctggcatgt tcagcaagcg ttaagcgcca
    14761 gtgacagcaa agacatcggt cttggatttg actgccgtgt gctgtatctg cgtgcgcagt
    14821 gtgccatcaa catcgaatca ccaaacgaca agctgaacag caaccttaat ctggtggcgc
    14881 gtgaactggc gaaggttcgc gataaaggtc tgccggaaga agagttcaat gcgttagtgg
    14941 cgcaaaagaa actggagctg cagaaactgt ttgccgccta tgcacgggct gataccgata
    15001 ttctgatggg ccagcggatg cgttcgttgc aaaatcaggt cgtcgatatc gcgccggagc
    15061 agtatcagaa actgcggcag gatttcctta acagcctgac tgtggagatg ttaaatcagg
    15121 atctgcgtca gcagttgtcg aatgatatgg cgttaatact gctgcagccg aaaggcgagc
    15181 cggaatttaa catgaaagcg ttgcaggcgg cctgggatca aatcatggcc ccatctaccg
    15241 ccgctgcgac cacctctgtc gccacggatg acgtacatcc tgaagtgacg gatattccac
    15301 ctgcacagta atgcctgatg gtgatgagtt agcacaacca tcaggtattt atggatgagc
    15361 tggatagagg ggtaacactt tatccagcct tttgcatatg ctgcgtttac gctggcatcg
    15421 cctcacgcgg gataatcgcg ccgcgatact gaataacggt acttgcggtc aggtgcccgc
    15481 gtttcgccgc gttttccgca ctgccgcccg tcaaacgtac cgccagataa ccggcgctga
    15541 aagagtcgcc agctgcggtg gtatcgatca ctttttcttt cggcagtttc accgccggaa
    15601 catcaactaa cgcttcacca gcaatggaca ccaggcaaga atccgccccg cgtttcacca
    15661 ccacttcttt cacgcccgcg ttatgggtgc gcgcaatgac gtcttccacc ggctgttgac
    15721 cccacagcgc gtcttcgtcg tccagcgtca ggaaggcgat atccgtgcat tccagcattt
    15781 gctggtacac ctgctgtgtc tcttctttgc tggcccacag gcgcggacga tagttattgt
    15841 cgaaaatcac ttttccgcct ttggcgcggc attcgcgcag cagggaaagc agcttttcgc
    15901 ggctggtcgg gcttaagatc gccaggctaa tcccgctcag gtagagataa tcgaaattcg
    15961 ccagctcttc gcaaatcgcc gcagactgct cgctcgccag ccagaatttg gcggcggctt
    16021 cgttacgcca gtagtagaac gtacgttcgc cggtgctgtc ggtttcaatg tagtaaaggc
    16081 ccggaagacg gttttccatc cgttgggtca gggaagtatc aacgttctcg ccgtgccagg
    16141 cgtccagcat ctgctggcta aaactgtccg ttcccagcgc cgttacgtaa tgaacggtta
    16201 atgccgcagg atcgacctga cgggcgatat agacggaagt gttcagggta tcgccgccga
    16261 aaccgcgctt aacgtccgcg tctttctcgg aaagctcaat catgcattcg ccaatcacgg
    16321 caatcttttt ggacatagtc gtgaacctga tctgtaaaaa attagcgtta gtttgcgctg
    16381 tggtggtttg ctggtcaacc atattaaaac agtgttccat tatttttttg agccagaacg
    16441 tattcctgaa agattccgtt gtggagtgag gaaatttacc ccggccaggc ggtaaagttc
    16501 tgcccttacg cgccgataat ctttgtcgag tccgggcagc atcactttta aacacaggac
    16561 atctttgatg ataaggcagg ttatccagcg aataagcaac cctgaagcaa gcatcgagag
    16621 cttgcaggaa cggcgttttt ggttgcagtg tgagcgtgct tacacctggc agccgatcta
    16681 tcaaacatgc gggcggttaa tggccgtgga gctattaacg gtggtcacgc atcccttgaa
    16741 cccttcgcaa cgcctgccgc cggatcgcta ttttactgaa atcaccgtca gccatcggat
    16801 ggaggttgtg aaagagcaga ttgatttgct ggcgcaaaaa gccgacttct ttgtagagca
    16861 cggcctgctg gcatcggtca atattgatgg ccctacgctc atcgccctgc gtcagcaacc
    16921 aaaaatcctg cgccagattg agcgtcttcc ctggctgcgt ttcgaactgg tggagcatat
    16981 ccgtctgccg aaagattcaa cctttgcctc gatgtgtgaa tttggcccgc tgtggctgga
    17041 tgattttggt accgggatgg caaatttctc tgcactaagt gaagtgcgtt atgactacat
    17101 caaaatcgcg cgagaactgt ttgtgatgct gcgtcagtcg ccggaaggcc gcacactctt
    17161 ttctcagctt ttacatctaa tgaatcgcta ttgtcgcggg gtgattgtcg agggcgtaga
    17221 aacaccggaa gagtggcgtg atgttcagaa ctcgcccgca ttcgccgcac aaggctggtt
    17281 tctttcacgc ccggcaccga tggaaacgct gaatacggcg gttctggcgc tataagctgc
    17341 ctcattttcc gccgggctgg actatcttta ggactggcac aggaagaata tgaggaagcg
    17401 aacgatgagc aaggcaggca aaataaccgc tgcgatttca ggggttttct tgttgttgat
    17461 tgtcgtggcg atcattttga ttgcaacttt tgactggaat cgactcaaac cgaccatcaa
    17521 ccagaaagtc tctgcggagt tgaatcgtcc gttcgctatc cgtggcgatc tgggcgtggt
    17581 gtgggagcgg caaaaacaag aaactggctg gcgcagctgg gtgccgtggc ctcatgtaca
    17641 tgcagaagac atcattcttg gtaatccacc ggatattccc gaagtcacga tggtgcattt
    17701 gccgcgagta gaggcaacgc tggcaccgct ggcgctgctg accaaaacgg tctggctgcc
    17761 gtggatcaag ctcgaaaagc ccgacgcgcg cctgattcgc ctctctgaaa agaacaataa
    17821 ctggacgttt aatcttgcca acgatgacaa caaagacgcg aatgcaaagc cgtcggcatg
    17881 gtcgtttcgg ctggataata ttcttttcga tcaagggcgg atcgccattg atgacaaagt
    17941 aagcaaagcg gatctggaaa tttttgttga tccgttaggc aagccgctgc cattcagcga
    18001 agttactgga tcgaaaggta aagcggataa agaaaaggtg ggcgattaca tttttggcct
    18061 gaaggcgcag ggacgttata acggtgaacc gctcacgggt acgggaaaaa taggcggtat
    18121 gctggcgctg cgtggcgaag ggacgccgtt tccggtacag gctgatttcc gttcaggtaa
    18181 tacccgtgtt gcttttgatg gcgtcgtgaa tgacccaatg aagatgggcg gtgtcgattt
    18241 acggcttaaa ttttctggcg attcactggg tgatctctat gaactgacgg gcgttctgct
    18301 gcccgatacc ccgccgtttg aaacggatgg tcggctggta gcgaaaatcg acactgaaaa
    18361 atcgtcggtc tttgattatc gcggttttaa tgggcgaatt ggcgatagcg atatccacgg
    18421 ttctctggtc tacaccaccg gaaagccacg accaaaactg gaaggtgatg tcgagtcgcg
    18481 gcaattgcgg ctggcggacc tgggaccgtt gattggcgtt gattccggga aaggggcaga
    18541 aaagtcgaaa cggtctgaac agaagaaggg cgaaaaaagc gttcagcctg cgggcaaagt
    18601 gctgccttat gaccgcttcg aaaccgataa atgggacgtt atggatgccg atgttcgctt
    18661 caaagggcgg cgcattgagc atggcagtag cctgccgatt agcgatcttt ctactcatat
    18721 catcctcaaa aatgctgacc tgcgcctgca accgctgaaa tttggcatgg cgggcggcag
    18781 cattgcggcg aatattcatc tggaaggcga taaaaagccg atgcaggggc gggcagatat
    18841 tcaggctcgt cgactgaaac tgaaagaact gatgcccgat gtggaactga tgcagaagac
    18901 gctgggggaa atgaacggtg acgcggaact acgcggtagc ggtaactcgg tggcggcgct
    18961 tttaggcaac agtaacggca acctgaaact gttgatgaat gacgggctgg tgagccgcaa
    19021 cctgatggag attgttgggc tgaatgtcgg caactacatt gtcggtgcga tatttggtga
    19081 cgatgaggtg cgggtgaact gcgcggcggc gaatctgcat attgccaacg gcgtggcgcg
    19141 cccgcagatt tttgctttcg atactgagaa cgcgttgatt aatgttaccg gcacggcaag
    19201 ttttgcttcg gaacagctgg atttgactat tgatccggag agtaaaggga ttcggattat
    19261 cacactgcgt tcgccgctgt atgtgcgtgg gacgtttaaa aatccgcagg ctggggtgaa
    19321 agccggaccg ctgattgccc gtggtgctgt tgctgcggca ctggcaacgc tggtaacacc
    19381 ggcggcggcg ttactggcac tgatctcacc ttccgaaggg gaggctaatc agtgtcggac
    19441 gatattgtcg cagatgaaga agtgatgata attgccggat gtctgacatc cggcaattaa
    19501 acagatttag atttttggca aagtgtgctt tgttcatgcc ggatgcggcg tgaacgcctt
    19561 atccggccta caaatccttg tgaattcaat atattgtatg gaaattcagt cctgataagc
    19621 gtagcgcatc aggcttttca cacttattac aacgactgat gtcgcgtctc atgggtcagc
    19681 agcagggcga ttaacgtcaa gccagccatc gccgccagat ataaccccac cgcacctaac
    19741 ccgtagttag cctgcaacca agctgcgata tacggcgcaa cggaagcccc gagaatcgac
    19801 gatacgttgt aagagaacga tgctccggtg taacgcactt ctgtcggaaa cagctctggt
    19861 aacagcgcac ccatcggccc gaaggtcagc cccatcagac ttaaccccag cagcaggaag
    19921 gcaaaaacca gaatcgggtt gccagaaccg agcagcgggt taaaggcgaa cagcgcgaac
    19981 aggatgatca gcgtggtgat gatcaccatg cttttacggc gaccaaaggc atcagccagt
    20041 aatccggcga ctggcaccat cacgccaaaa ccaataactg ccatcatcag catccacaac
    20101 acttcgttac gcggcaggcc aagcccaact ggcgcggcgg cggtactgaa ggtcatagag
    20161 tagaccgtca tgatgtaaaa cagcgtatag gttgccagca taatgaacgt acccagtacg
    20221 gttacgcgaa catgtttggt cagcagcgta cccagcggga tcttcacctg ttttttcgct
    20281 ttagcgactt tctcaaacac cggcgactca tgcagcgaca cgcgaacata caggccgata
    20341 atgaccagca ccgccgagaa gataaatggc acacgccagc cccagctcat aaactgctca
    20401 tcggtcagta gccaggaaag cagcaagaaa gtgccgttgg caaagaagaa gccaatcggt
    20461 gcgcctagct gtgggaagga gccatacagt gcacgtttgc gcggtggggc gttttcagtc
    20521 gccagcagcg ccgcgccgcc ccattcaccg cctaagccca gaccctgacc aaatcgagcc
    20581 agcgccagca gcagtggggc gaaaatacca atcgtggcat agcccggcag cagaccaatc
    20641 accacggtcg aaatccccat cgttagcaac gaggcgacca gcgtcgcttt acgcccaacg
    20701 cgatcgccaa aatgaccaaa aacggcagag ccaatggggc gcgcgacgaa ggcgatggcg
    20761 aaggtggcga gcgactgtag cgttgctgcc gttggatcgc cctgcgggaa gaagatatgc
    20821 ggaaacacaa taacggccgc agtggcgtaa atgtaaaagt cgaagaactc aatggccgtg
    20881 ccaatgagag aggcgacaag gactttatta cgcgagttga tcggcgtgta ttcttgttcg
    20941 tggtcgagtg ttgtggctgt tgcttgcata atcttttctt attttttggc taacgaatag
    21001 ccatattacg cacagcaaaa gtgatatttc aatgtgtaac aaatcgcgga aatggtcaaa
    21061 aaccaggcaa aaaggggata atgtttaggc cagtgatttt tcttctatga aatgcttcac
    21121 ataattttat aaatagtgga taaaactggt ttcaggttaa ataaaagtta ccaactggat
    21181 ttatatgctg gggttatgag ttggagtgaa attgtttgct cccaaataga tggctgaaga
    21241 ggctctgggg aagagtttca agaggttatg tgcgcattat agccggatgc gatgctggcg
    21301 catcttatcc ggcctacggg gagccaggcc gaataaaatc gtatgccgga taaggcgttt
    21361 acgccgcatc cggcctattt taaggctgcg ttttccctgg cattcgcggg tcgtctttat
    21421 attcggcggt ggcaatccac gccgcgcaaa acagcgtcaa acgggcgaag aagtagaaaa
    21481 atgccatcag ccccagcacg gaaccaaatg ctgcgccaga gggggatttc atcagcgatg
    21541 gcagggtgta ggtcatcacg attttaatca cttcaaaacc aatagcagcc agaaatgttc
    21601 cgcgaatcag ggcttttttg cgcggacggt ggcgcggcaa ccgccagaag atccagaaga
    21661 aaagcagata gttggcgaag atggaaatcg ccaggccaat caatcgccac gtcggtttca
    21721 gccactcaat gctgttcagg tgcagggcgc taataatcat ttgctgcgcc gaaccggcaa
    21781 ccgaggtgat cgaaagcgtc acaatcagcg caatcaacaa accaatcagt gaaataaaat
    21841 cacgcagata ttttacccag aacttctcct gatcttgcgg cgagcgttcc cagacatcgc
    21901 gcgactgggc acgaatcgct tcacgcaggt tacccatcca gttgatgccg gaataaagtg
    21961 ccaccgccag gccgacaagc cctacagtcg tacgctgctg aacggcggtg ttgatggtgt
    22021 ttttcaacgt ggcggctagt gtcggatcgc tgatgttttg cagaattttg tcgaagatat
    22081 cctgtagcaa catcggatgg gaggccagca caaagccccc tgcggcaaac gacaccatca
    22141 aaatcgggat catcgacaaa aatgagaaat aggtgatagc cgcaccaaac tggttaccca
    22201 gccgatcgtt aaagcgttct gtcgcgcgaa taaggtgcgc aataaccggt tggcgttgga
    22261 ctttttcggc agtggtggtg acggtttcca gcgcctggct aactttgccg cctttctcgc
    22321 tattatccaa ctgcttaatc ggctcgtgct ccagatcctg ggtgggacgt ttgatctcgt
    22381 tttcctgcgt catccgctct tttgtccttc tgtcgtggtc attcttaaaa agtatagtcg
    22441 gtcagtccag gtgctctttc attacgccag ccagccactg cataaacagg tttacacgcc
    22501 gggaaagctc ccgacgctgc gggtaaacca gcgaaaggga gagcggctcg gcacggtagc
    22561 ctggcaacac ttcaataagc cgcccggcac gcagggcttc gcgcacggca atgcgcggga
    22621 tctgaataat ccccagaccg gcaagaccgg cggcgagata gttttcgcta ctgtttaccg
    22681 tcaacatgcc gccagactta aaccactgga cgccattaac gctggcaacc tcaaaaccta
    22741 acggatgtac acccaggtgc ggtgtataac gcactattgc gtgtgaagtc agatcatcgg
    22801 ggctttgcgg ataaccaaag cgcgtcagat agtgcggact ggcacagttg accatggtta
    22861 gtttgccgag gggacgggcg ataacgccat cttccggtaa cgcgccagtg cgtatcacgc
    22921 aatcaaaacc atcatgaaga atatctaccg gacggttatg gctactcagt tccagctcaa
    22981 ttcccggatg ctgatagaga aattccgaca gacgcggcag taacaggttt ttcgcgattc
    23041 ctgacgggat gtcgatgcgt aatttaccgc tgatactggt ggcatcctgt tgaaacagac
    23101 cgtccagttc gctgagatta ctcaacacat cttttgcccg ttgataatag gtcatgcctt
    23161 ctggcgtgag tttgacccgt cgcgtagtgc gctggagaag ctgggtgcca agttgatgtt
    23221 ccagtgcctg tatctggcgc gaaacacttc cctttggcaa agcaaagaaa tccgccgcgc
    23281 gggaaaaact ttccagctcc gcgactttga tgaacaactg cattgcgtga attttatcca
    23341 tttctttgac cgattgttgt tcacagcgaa acagtaacgc gcatatcgca ttgtttattg
    23401 ttttttctaa catctaatgc cgggtttcac aatgttgtca tgacgaatgt ggcgatttct
    23461 cagcaggaaa agggcggaat agctttatga gaaaggtgaa tccccttaaa cagagcgtat
    23521 ttttcagtga ttccctttaa agtgaataga ggacgtattg gctatttgtg cttatttttg
    23581 tctcattgcc atttgttatt ttggcttgta gtcgcggaat ttcattttta attgcatgcg
    23641 tactaaccac caatataagg aaatcctcaa aattgtttta aatagtgccc tcgacatgta
    23701 atttcaggcg tttagctgta actatttata tcggtaacta ttttctacgc gtcatctctg
    23761 tagaatggcc accgccaaat ggcgaattct tattttttct cgcgggtaat acccttccac
    23821 tacttaagtc tttttaacgt attcaggtgg agattcagtt atgcaaatag tcatgtttga
    23881 caggcagtca atatttattc atggaatgaa aatcagttta cagcagcgta ttccaggagt
    23941 gagtattcag ggggtcagtc aggcagacga gttatggcaa aagctggaaa gttaccctga
    24001 agccttagtt atgctcgatg gcgatcagga tggtgagttt tgctactggt tgctgcaaaa
    24061 aaccgtggtg cagtttcctg aggttaaggt gttaatcacg gcgacggatt gcaataaacg
    24121 gtggttacag gaagttatcc attttaatgt gctggccatt gtgcctcgcg attcaaccgt
    24181 cgagacattt gcgttggcgg taaatagtgc ggcgatgggg atgatgtttc tgcctggaga
    24241 ctggcggacg acgccggaaa aggacattaa agatctcaaa tcgttaagcg cccgccagcg
    24301 ggagatttta accatgttag cagcgggcga atcaaataag gagatcggca gagcgctgaa
    24361 tatcagtacc ggaacggtaa aagcgcatct ggaatccctg tatcgccgtc tggaagtgaa
    24421 aaatcgcacc caggcggcaa tgatgttaaa tatctcctcc tga
//
LOCUS       DAJTNM010000050        24460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTNM010000050 DAJTNM010000000
VERSION     DAJTNM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.050"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..543)
                     /gene="fdhD"
                     /locus_tag="OO172_002131"
     CDS             complement(<1..543)
                     /gene="fdhD"
                     /locus_tag="OO172_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5512316.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGE"
     gene            737..3787
                     /gene="fdnG"
                     /locus_tag="OO172_002132"
     CDS             737..3787
                     /gene="fdnG"
                     /locus_tag="OO172_002132"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1322..1324,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5512317.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMFSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGITLTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            3800..4702
                     /gene="fdoH"
                     /locus_tag="OO172_002133"
     CDS             3800..4702
                     /gene="fdoH"
                     /locus_tag="OO172_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5512318.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            4699..5334
                     /gene="fdoI"
                     /locus_tag="OO172_002134"
     CDS             4699..5334
                     /gene="fdoI"
                     /locus_tag="OO172_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5512319.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5331..6260
                     /gene="fdhE"
                     /locus_tag="OO172_002135"
     CDS             5331..6260
                     /gene="fdhE"
                     /locus_tag="OO172_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5512320.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6590..6832)
                     /locus_tag="OO172_002136"
     CDS             complement(6590..6832)
                     /locus_tag="OO172_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512321.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7050..7268)
                     /locus_tag="OO172_002137"
     CDS             complement(7050..7268)
                     /locus_tag="OO172_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5512322.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(7947..9080)
                     /locus_tag="OO172_002138"
     CDS             complement(7947..9080)
                     /locus_tag="OO172_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512323.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKP
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATVVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            complement(9250..9588)
                     /locus_tag="OO172_002139"
     CDS             complement(9250..9588)
                     /locus_tag="OO172_002139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512324.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(10178..11119)
                     /gene="fabY"
                     /locus_tag="OO172_002140"
                     /gene_synonym="yiiD"
     CDS             complement(10178..11119)
                     /gene="fabY"
                     /locus_tag="OO172_002140"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5512325.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(11164..11601)
                     /gene="dtd"
                     /locus_tag="OO172_002141"
     CDS             complement(11164..11601)
                     /gene="dtd"
                     /locus_tag="OO172_002141"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5512326.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11598..12470)
                     /locus_tag="OO172_002142"
     CDS             complement(11598..12470)
                     /locus_tag="OO172_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5512327.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12464..13063)
                     /gene="yihX"
                     /locus_tag="OO172_002143"
     CDS             complement(12464..13063)
                     /gene="yihX"
                     /locus_tag="OO172_002143"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5512328.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13162..13946)
                     /locus_tag="OO172_002144"
                     /pseudo
     CDS             complement(13162..13946)
                     /locus_tag="OO172_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(13980..14876)
                     /gene="yihV"
                     /locus_tag="OO172_002145"
     CDS             complement(13980..14876)
                     /gene="yihV"
                     /locus_tag="OO172_002145"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5512329.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGVKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            15809..16699
                     /gene="yihT"
                     /locus_tag="OO172_002146"
     CDS             15809..16699
                     /gene="yihT"
                     /locus_tag="OO172_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5512330.1"
                     /translation="MPLGAASREVYSQARASGGFAMLAVDQREAMRMMFAAAGAPAPV
                     ADSVLTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNG
                     IPVDSVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVS
                     IIEPVVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRL
                     NDHINMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDV
                     CAPKLQQLGDIVDEMMAKRR"
     gene            16716..17957
                     /gene="yihS"
                     /locus_tag="OO172_002147"
     CDS             16716..17957
                     /gene="yihS"
                     /locus_tag="OO172_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5512331.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            18095..18997
                     /locus_tag="OO172_002148"
     CDS             18095..18997
                     /locus_tag="OO172_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5512332.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            19196..21232
                     /gene="yihQ"
                     /locus_tag="OO172_002149"
                     /pseudo
     CDS             19196..21232
                     /gene="yihQ"
                     /locus_tag="OO172_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            21278..22663
                     /locus_tag="OO172_002150"
     CDS             21278..22663
                     /locus_tag="OO172_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5512333.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            22706..24109
                     /locus_tag="OO172_002151"
     CDS             22706..24109
                     /locus_tag="OO172_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5512334.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            24177..>24460
                     /gene="ompL"
                     /locus_tag="OO172_002152"
     CDS             24177..>24460
                     /gene="ompL"
                     /locus_tag="OO172_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5512335.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGL"
BASE COUNT         6052 a         6274 c         6022 g         6112 t
ORIGIN      
        1 ttcgccagat ggcaacatcc aggcagcggc gtgagtacaa ccagtcagtt gccccactgg
       61 ctgaaaatcg ttgagatgac gtaatgcatc atccagtttg ttgagatcaa acgtctgggt
      121 gaacggtagc ggctgcaccg gttttccgat gtcattaagt tgctccacgc cgcatacgcc
      181 acagcccgta cgtcccgcca gcgcccggcg gcgctccttc aaccccataa agcggcggct
      241 ggaaagctca atttgtactt caagaccatt acaggaagga acgacatcca tgccgaagat
      301 atcgcgcgga ctttctatta tcccctcgga aagcgaaaaa ccgagcgcaa agtactcaag
      361 gtctttgggc gacgccatca tcaccacatg cgaaatgccg ttgtagacca gcgcaacggg
      421 aacttcttcc gcgacctcat ctaaccgtgg gtgttgtaaa tcatcacgcc gccataactc
      481 aatttgacga acacctgtga tatttgtcac attttcgatt tcttttcgct gtgttttttt
      541 cactttctct taaccagata agaacacaca caccaacatt atggtattct gttacaaacc
      601 cttcctagat ggagggaaat tgagccaatt ctggaccttt gcggcccctt ccgcaaagaa
      661 aaataactcc cactccctgc acacgcagca agcgaatgta aatgggacgt gacaatgtcg
      721 aaacaaggag caatccatgc aggtcagcag aaggcagttc tttaagatct gcgctggcgg
      781 tatggcaggc accacggcag cggcactggg ttttgcaccc agcgtagcac tcgcggaaac
      841 ccggcagtat aaactgctgc gcacccgcga aacccgtaat acctgcacct attgttccgt
      901 aggctgtggg ctgttgatgt tcagcctcgg tgacggagca aaaaacgcca aagcatctat
      961 cttccatatc gaaggcgatc cggatcaccc ggtcaaccgc ggtgcacttt gtccgaaagg
     1021 cgctggcctg gtggatttca tccactccga aagccgtctg aagtttccgg aataccgtgc
     1081 gccaggttct gataaatggc agcaaatcag ttgggaagag gcgtttgatc gcatcgccaa
     1141 actgatgaaa gaagaccgcg atgctaacta cattgcgcaa aacgccgaag gcgtgactgt
     1201 taaccgctgg ctctccaccg ggatgctgtg tgcttccgcg tcgagtaacg aaaccggcta
     1261 tttaacgcaa aaattctccc gcgcgctggg tatgctcgcg gtcgacaacc aggcgcgtgt
     1321 ctgacacgga ccaacggtag caagtcttgc tccaacattt ggtcgcggtg cgatgaccaa
     1381 ccactgggtc gacatcaaga acgccaacct cgtcgtggtg atgggcggta acgccgctga
     1441 agctcacccg gtcgggttcc gctgggcgat ggaagccaaa attcacaacg gcgcgaagct
     1501 gattgtgatc gatcctcgct ttacgcgtac ggcggcggta gctgactact atgcccctat
     1561 tcgttccggt actgacattg ctttcctgtc aggcgtattg ctgtacctgc tgaacaatga
     1621 aaaattcaac cacgaataca ccgaagccta taccaacgcc agcctgatcg tgcgtgagga
     1681 ttacggcttt gaagatggcc tgttcaccgg ctacgacgcg gaaaaacgca agtacgataa
     1741 atcctcctgg acttatgaac tggacgaaaa cggcttcgcc aaacgcgata ccacgctgca
     1801 acatccgcgc tgcgtgtgga acttgctgaa acagcacgtt tcccgctaca cgccagatgt
     1861 ggttgaaaac atctgtggta cgccaaaaga cgcgttcctg aaagtctgcg aatacatcgc
     1921 agaaaccagt gctcacgata aaactgcctc gttcctgtat gccctcggct ggacgcaaca
     1981 ctccgttggt gcgcaaaaca ttcgtacgat ggcgatgatc cagctgctgc tcggcaatat
     2041 ggggatggcc ggcggcggcg ttaacgccct gcgcggtcac tccaatattc aggggctgac
     2101 ggacctgggg ctactgtcgc agagtctgcc aggttacatg acgctgccaa gcgagaagca
     2161 gaccgatctg caaacctacc ttaccgccaa cacgccaaaa ccgctgctgg aaggccaggt
     2221 aaactactgg ggcaactacc cgaaattctt cgtctctatg atgaaggcct tctttggtga
     2281 taaagcgacg gcggaaaata gctggggctt tgactggttg ccgaagtggg ataaaggcta
     2341 cgacgtcctg caatacttcg agatgatgaa agagggcaag gtcaatggct atatctgcca
     2401 gggctttaac cctgttgcct cattcccgaa caaaaacaaa gtgatcggtt gtctgtcgaa
     2461 actgaagttc ctcgtcacca tcgacccact gaacactgaa acctctaact tctggcagaa
     2521 ccacggtgag ctgaacgaag ttgactcgtc gaagatccag accgaagtgt tccgtctgcc
     2581 atcgacctgc ttcgcagaag agaacggttc aatcgttaac tcaggtcgct ggttgcagtg
     2641 gcactggaaa ggtgcggacg ccccggggat tacgctgact gacggtgaga tcctctccgg
     2701 tatcttcctg cgcttgcgca agatgtatgc cgaacagggt ggcgcgaacc cggatcaggt
     2761 gctgaacatg acctggaact acgccattcc gcatgagccg aagtcggaag aagtagcgat
     2821 ggagagcaac ggtaaggcgc tggccgatat taccgatccg gcaaccggtg cggttatcgt
     2881 caagaaaggc caacaactta gctcgttcgc ccaattgcgt gatgacggta caacctcctg
     2941 tggctgctgg attttcgccg gtagctggac gccggaaggc aaccagatgg cgcgtcgtga
     3001 taacgccgat ccgtctggcc tcggtaacac gctgggctgg gcatgggcat ggccgcttaa
     3061 ccgccgcatt ctgtataacc gcgcctccgc agatccgcag ggtaacccgt gggatccgaa
     3121 gcgtcagttg ctgaaatggg acggcactaa gtggaccggc tgggatattc cggactacag
     3181 cgcagcgcct ccgggtagcg gcgtcgggcc gtttatcatg cagcaggaag gcatggggcg
     3241 tctgtttgcc ctcgataaga tggcggaagg tccgttcccg gaacactacg agccgtttga
     3301 aacgccgctg ggaactaacc cgctgcatcc aaacgttatc tcgaacccgg ctgcacgtat
     3361 ctttaaagac gacgccgaag cattgggtaa agccgataag ttcccgtatg tcggaaccac
     3421 ctatcgtctg accgagcact tccactactg gaccaaacac gcgctgttga acgcgatttt
     3481 gcagccagag cagtttgtgg aaatcgggga gtcgctggcg aataaacttg gcattgccca
     3541 gggcgatacc gtgaaagtct cctccaaccg tggctatatc aaagccaaag cggtggtgac
     3601 caaacgtatt cgcacgctga aagcgaacgg caaagatatc gataccatcg gtattccgat
     3661 tcactggggc tatgaaggcg tagctaaaaa aggctttatt gccaatactt taacgccatt
     3721 cgttggtgat gcgaacacgc agacgccgga gtttaagtcc ttccttgtga atgtggaaaa
     3781 ggtgtaacgg agacgacata tggcttatca atctcaagat atcattcgtc gttccgcgac
     3841 taacggtctg acccccgcgc ctcaggcgcg ggacttccag gaagaagtgg cgaaactcat
     3901 cgacgttacc acctgtatcg gctgtaaagc ctgtcaggtg gcgtgttcag agtggaacga
     3961 cattcgcgat accgtcggca ataacattgg ggtgtacgac aaccccaatg atttaagcgc
     4021 caaatcgtgg acggtaatgc gcttctcgga agtggagcag aacgacaaac tggaatggct
     4081 gatccgcaaa gacggctgta tgcactgttc cgatccaggc tgcctgaaag cgtgcccggc
     4141 ggaaggtgca attattcagt atgccaacgg tatcgtcgac ttccagtccg agcagtgcat
     4201 tggctgcggt tattgcattg cgggctgtcc gttcgacatt ccgcgcctca acccggaaga
     4261 caaccgcgtc tacaaatgta cgctgtgcgt tgaccgcgtg gtggttgggc aagaaccagc
     4321 ctgcgtgaag acctgcccaa cgggcgcgat tcacttcggt acgaaagagt cgatgaaaac
     4381 gctggcgagc gagcgcgttg ctgagctgaa aacccgcggt tacgacaatg cgggtctgta
     4441 cgatccggca ggcgtcggtg gtacacacgt catgtacgtg ctgcaccatg ctgacaagcc
     4501 aaatctgtat catggcttgc cggagaaccc ggaaatcagc gaaaccgtga aattctggaa
     4561 aggcatctgg aaaccgctcg cggctgttgg ctttgcggct acctttgcgg ccagtatctt
     4621 ccactacgtg ggtgtcggtc cgaaccgtgc ggatgaggaa gagaataatc tgcacgaaga
     4681 gaaagacgag gagcgcaaat gaaacgacgt gacaccatcg tgcgctacac cgcgccggaa
     4741 cgtatcaacc actggatcac cgccttctgc ttcatcctgg cggcggtgag cgggctgggc
     4801 tttttgttcc cgtccttcaa ctggttgatg caaatcatgg gcacaccgca gctggcgcga
     4861 attctgcacc cgtttgtcgg cgtggttatg tttgcctcgt tcatcatcat gtttttccgt
     4921 tactggcatc aaaacctaat caatcgggat gatatctttt gggcgaagaa tattcgtaag
     4981 atcgtcgtca acgaggaagt aggtgacacc gggcgttata acttcggtca gaaatgcgtt
     5041 ttctgggcgg cgattatttt cctggttctg ctgctggtga gcggcgtgat tatctggcgt
     5101 ccttattttg cgcctgcttt ctcaatcccg gtgatccgat tcgcgttaat gctgcattca
     5161 tttgccgcag tggcgttaat tgtggttatc atggtgcata tctacgccgc cctttgggtg
     5221 aaaggcacga ttaccgccat ggtggaagga tgggttacca gcgcctgggc gaagaaacat
     5281 cacccgcgct ggtaccgtga ggtccgcaag acaacggaaa agaaagctga atgagtattc
     5341 gcataatccc gcaagatgag ctgggttcga gcgagaaacg tacggcgaat atgattccgc
     5401 cgttattgtt ccctcggctc aagaatttat acaaccgccg cgccgagcgt ctgcgcgagc
     5461 tggcagaaaa taatccgctg ggtgattacc tgcgctttgc tgcgcttatc gcccacgccc
     5521 aggaagtggt gctgtacgac catccgctgg agatggatct gaccgcgcgc attaaagaag
     5581 ccagcgcaca gggcaagccc ccgctggata ttcacgttct gccgcgtgat aagcactggc
     5641 aaaagctgct gatggcgcta attgctgagc tgaaacctga aatgagcggc ccggcgctgg
     5701 cagtgattga gaatctggag aaggcatcga ctcaggagct ggaagatatg gccagcgcgc
     5761 tgtttgcctc tgatttctcg tccgtcagca gcgataaagc gccgtttatc tgggctgcgc
     5821 tgtcgctcta ctgggcgcag atggccaatc tgatccccgg caaagcccgc gctgaatacg
     5881 gcgaacaacg tcaatattgc ccggtatgtg gctctatgcc ggtgtccagc atggtgcaaa
     5941 ttggcaccac tcagggtctg cgttacctgc actgcaacct gtgtgaaacc gaatggcacg
     6001 tagtgcgcgt aaaatgcagc aactgcgagc agagcggcaa actgcattac tggtcgctgg
     6061 atgacgaaca ggccgcgatt aaagccgaaa gctgcgatga ctgcggcact tacctgaaga
     6121 ttctttatca ggaaaaagaa ccgaaagttg aagccgtggc agatgacctc gcctctctgg
     6181 tactggacgc gcgaatggag caagaaggct acgcccgtag ttccattaac ccgttcctgt
     6241 ttccgggtga aggagagtaa tctctgacgt tttccgggtg gcgcaggtcg cccggattac
     6301 agctcatagg gtgtgacgac accatctctc gtattccgcg ttacccattc aagatcatgc
     6361 cgcaattgtc tgtttgcctc gttcttccct ccaacacatc gataaggtta tgggtatgtc
     6421 actgatgaca agcgtatgat tgcctgatgc gctgcgctta tcaggcctac caataaactt
     6481 caatatattg aatttgcatg actttgtagg ccggataagg cgttcacgcc gcatccggca
     6541 tgaacaacgc gcacgttgtc agcaatctga ttgacatcat tttttcccat taccattcct
     6601 cgcgcagctt acgctgatat tcgacaccat cttcctcaca cccttgccag atgccaggaa
     6661 cactggttct tgctgtttgc gattgattta tcaggtattg atctaccgct tcccttaata
     6721 ggtccgcacg aggaagatta cgctgcacct cgagatcatc aagttgctta atcacctcat
     6781 tcgataaatc gagtaaaatt ctgcccatat ctatacctgc cagagagttc ataaatatct
     6841 cgccaatatc atttttgaat ctatggagag aaaagtaccc ttgccgaatc tttaaagaaa
     6901 gctcatttac gcatcatttt ttatttttgc gaggcgtttt ccagatacga tgtaacagtt
     6961 gcagttatta taagttcaag taaaacagcc ttgtattaat accagaataa gggcatctgt
     7021 tttttatatt caagaatgaa aaattttttt cattccttat gctccttaca ctgatcatca
     7081 acaccaacta cctccccctt ttgcctgcct ccctttgctt tacgcaccgg aaattgctgc
     7141 cgctccagat attgctcaac cgcttcgcgt aaaattgcag atcgtgaaac cttacgccag
     7201 ccccgaagct tgttcagccg tttaattgcc tcttctgata aatgaagaaa aaccgtattc
     7261 atcgccatta ccaccacctt acatatatta ctcattaatg tatgtgcgaa aattacgccg
     7321 aaaaagcatt taaaaaaatg cggatttttg catgtcattt tgtttttgcg aggcgttttc
     7381 cagaaataat aagagcgttg cagttgtttc atttattact caaaaatggt ttagttatcg
     7441 cccaggatga gttatgggtg ctatgaatat attatattca cggatattta tttgcataat
     7501 ctgaatggtg tagccgaaaa acgtcagcat cctaacagca ccaggattta aggtgaaatt
     7561 aatctttcat cacagaccgg tcagtactgc caccaaccgg tctttacttg ctcatcgtta
     7621 taattttgtt ggtaacagtt cgggaaacaa ggaattcctt ccggaacacc cccggcatca
     7681 tccgatagca ttgacgtcaa tcatcagcgc gacgatgagt ttttttaatc gcatcatagg
     7741 gtggcgacgt tcagcacgat tacgggtcat agcaaaactc ctgagcaggc ggccactatg
     7801 ccgctttcac tggagttctg aatactggta ctttctgtat ggcttcgcca tgcccctcgc
     7861 ggctaagtac ctgcacacat ctctctttgt atgtcatttt tgcaatgcat tgtccgaatt
     7921 ctgataatcc taatgagttt tactcgtcag actcccatcc ctacggtata cgagttatat
     7981 tcatgaatga aatcgacatt tttaaaaaac gaatcttgta aaacatcagt gttgatatta
     8041 ccttgcaatc caacattgaa tttgttagag acatgaagaa ctcgaatatc aaaaaggttt
     8101 tttaacatca ttaattcttc gggtgttaat gaatcgcaac taaaatatcc ctgttgatca
     8161 aaaagatttt ctatttctcc actatcggcg aggcgttttt caaggagtgt ttgcaacatc
     8221 gagttgccaa ggacaatgga tatcgtgcga tctttcgcag atttacaccc catcacaacg
     8281 gcaacattcg tatccccctc aagagtttca tttatcgccg tcgctaaaat ggtggtcaaa
     8341 cacgcaaacg gaactgtatt tctttcgtga tttaatttat ctaccagttc attccattta
     8401 tcaaaagccg ggctatattt gttgcgcaca gaaatatccg actggttaaa caattcagcc
     8461 atgcgatttt ttatcatctc gatatccggc gaaccagaaa gactttgcgc ataatacggt
     8521 cctgatgatg ttgcatacat taatgccaag cccacaactg tcgccatgat gggcgcagcc
     8581 acggttttgg caagggcgac gctgcgatgg ctatgcgctt cacaggctaa aggaacatat
     8641 tccactgact gcggtaattc cttacatgcc tccctcatca ttgcggtatg ctttacaaac
     8701 aacggttttt cactgcccaa aaaaactttc tgcttaattg cactaagaaa acgcaaatca
     8761 aaaataattg tttttggttt attatttttt ggcacatttc ctattatccc gctaagaacc
     8821 ttccttgctg catggagatt tttttgcaca gcacataaca caattgatga agccttctcc
     8881 actgcattat tgattttaat tttaaaatcc gcatgatata cgttgacggg caatgcaagt
     8941 atatctggac ctacatcttt tgctgagtat ttattggtga tcgacggacg tgaacccacg
     9001 agaaaactta gctgcaaatt atgctctata ttatcagctg tttttgtggt gtggttaaac
     9061 atagagatgc cacctttcat tctttcacac atatcctcaa ctttttcact tcgttcttgt
     9121 ggcaatttgg ctttaacatt aatttgatga tttttacccg tgcattcctt aactacaagg
     9181 ggaatgttat ctaaagtgcc atacaggcta ataacgtcac caggattttt atcatgtaag
     9241 taagtttgat taatacttga agcattcaca aagagggtca atattataac tgtttataac
     9301 ttcacctaca tcaagtgtgt agtgcttact ctggagactg ccatccttat ttaattgtat
     9361 atcaatcaat atatccccgg actttgtcac catatctttg ccataaacca tttgcatagc
     9421 catttcgagg agatttttat ctaactgatt gcgggcgttg agtaagcgat taccaaataa
     9481 tttatatact gttcccgtca ttgccagcgc tgcgctttct ggtactttgc gtttccacag
     9541 tgaggtttct ctatttttaa acaggtcata ttcattatta ctcgacaaac catgcttcag
     9601 ttcgttaaca caatcgcaca gttgctcctc accttccatt ttaaggcaaa gtgaaaattg
     9661 atttaatggc gtttacttcg gtgatttatt actcattagt tgtttttcta aattaacaac
     9721 agaataactt tcttgtttaa tgagattaat attggcaacc attatagcct ccttcccacg
     9781 gtttaagagc aaacatacaa cataatgatt cataacatac agagcagtaa atgaatgata
     9841 ctgacaatgt atgccagccc attatctttg cataatgttt gtgagctata gattttaaaa
     9901 atatttatga aaaaagtatg gcaccagagg gggttttgac tatcaaaaat cgctcaaacc
     9961 tgaactaaga tgatgagtta agcattatct gcaataattg ccactcagct ctacaaataa
    10021 tatttaaatg ctcctgaaca gtgatgtagc aagacacgta tggtgccaac gaagaataaa
    10081 aatttataaa gttcttattc aatataaccg gaatagtcag ggactgatgt aaatgaccgg
    10141 aatgaatgag agaggcagca ccgcccctct ccataagcta ctcttcttcg ttcccgccct
    10201 cttcatacgg gccaaatggc ttcgcgggca gaacgatata cgtgccttca aacactgcac
    10261 ccggcgtctc gtcgccaaag atttcgacct gcatctgcac ccgtgctttt cgtccgcgcg
    10321 ccagacggtc gagatcgccg cttaaggcac caaggtcggc taccgcatgg ggtttaccgc
    10381 taatcggttt gctgtagcgg atatgcgcat ccgccagaat aatcgttccg ccgaggtggc
    10441 gttcacgcag catcagccag ataagccccc aaccggtgag cgtcgccagc gagaataaac
    10501 tcccggcaaa cagcgtatgg tgcggattct gattgccggt ttccggcatg gtggtgataa
    10561 atttttgccc ggtatattgt tgaatacgca cgcccatttt ttcactaagc gggatatgtt
    10621 cgtaccacgc ctgttgcagc tgcgcgcacc agtcgccgcg atgcaaaata tcatccagag
    10681 tggcgacggg cttaatcatc aaaaaatggc gaatcggcgt ggtggttggc gtggtgattt
    10741 ctccctgatt aacaaacccc agcttggcga aaaactccac cgcgtcttca cgggcgctac
    10801 aggtcacgcg cttaacgcct tcctgacgcg ccaccgactc cagggtcatc gccatcagcg
    10861 ttcctaaccc tttgtcctgc acgtccgggt gaacggccat aaagcgaatg gacgcttcat
    10921 tgtcggcatt aatatacaat cggcctaccg ccaccagatt accctgctcg tcgacgacca
    10981 tctggtgatg cgccatcgca tcccacgcgt cgcgttccga accttttggt tgatgcaggg
    11041 gcttacgcaa catttcccag cgaaactgat agtaacgctc taactcttct tctgtttgtg
    11101 gaacctgaag gtgatacata gcgatactct ctcttgtttc ccgtgacaac cctggaagct
    11161 ggctcatacc tgcaaccaga atgtcacggg gccatcattg accagcgata cctgcatatc
    11221 ggcagcgaag cgtcctgttt gcgtgttcat ctcttgctgg cggcagcgtt cgacgaaata
    11281 gtcatataac gcctctgcgc gatccggtga tgcgcctttg gagaaacttg ggcgcatccc
    11341 ccgttcggta tctgcggcga gggtaaactg ggaaaccacc agtacactgc cgcccgcctg
    11401 ttgcacgttg agattcatct tgccttcggc atcgctaaag atgcggtagc cgagcacacg
    11461 ctcgcacaga cggtttgctt tctgttcgtc gtcatccttt tcgacaccca ataacaccaa
    11521 aagtcccgcg ccaatttcgc ccgtcacttc tccctccacg gtgacgctgg cacgggttac
    11581 gcgttgaatt aatgcaatca tggttcgtcg tcttcttctt gttcagctgc ttgttttagt
    11641 ttgcggtatt ccccgagagt gacagtaatt tccgcgccaa gcaagacgat acaccatgtc
    11701 cagtagaccc aaacaaagag aatggggatc accgccagca caccgtaaat gagctgatat
    11761 gacgggaaca tggtgatata aagcgcgaaa cctttctttc ctgcttcgaa caggagtgcg
    11821 gcgacaaacg cgccgacaat cgcgtcgcgg ttaggtacgc ggatggtagg aacaatgctg
    11881 tacagcaacc agaaggagat ccacgacaac agcaacggaa aaatacgcag cacgttgtcg
    11941 atgacagtat tgagatcgct cgcccagcgc agggagagca aataggaact gatcgccaga
    12001 ctggcccctg ccagcagcgg ccccagcgtt aaaatcatcc agtacacggc gaacgagtaa
    12061 atttttggtc gcgctcgttt actgcgccag atggtattca acgcgctatc gatggagtac
    12121 atcaacaata acgcggtgac gatcagcccg cacgccccaa cggcggtcat cttgttggaa
    12181 ttagcaacaa attgttcgat ataccgctga ataacatcgc cagtagcagg cagaaagttg
    12241 gcaaaaataa agtgacgtaa ctgaatgctg acgtcggaaa acatgggaaa agcggcgaaa
    12301 agcgcaaaaa caacggcaac cagcggcact aatgagagca acgacacata ggcaaggtta
    12361 cctgccaggg ttgtcatgtt gtcctcatca atacgttgcc agagtagttt tagccaggcc
    12421 catagtggac gggtacggtg cctggctttg tcctgaatgg tttttagcat aacaccttcg
    12481 cgaaatagtc cgggatggtg gttttatctt tcaccagaat actggtaatg cctagctgat
    12541 tagctccttc tatattatcg gcgttatcgt cgaaaaagac cgtatcgctg ggtgaaaaac
    12601 cttccgcctg caaaacatgc tggtaaattc gtgcttcagg tttgcgcatc cccagatctt
    12661 gcgacagata gatatggtca gcagcatcac gaatttccgg gtattcttcc ggccagaagg
    12721 tggtatgcag gcggttggta ttggaaagca ccaccacgcg atgcccctgc tcacgcagtt
    12781 tatgcatgat ggcgatcact tccgggcgca gcgcaacaaa caccgcctgc cagccgtgag
    12841 agaactgctc gtagcttagc ggtagagcca tctcatgaca cagcgcctct gcgaacgctt
    12901 cgtcgctaat ttccccacgc tcatgctgat gaaacgcctc ccccatatga aaactcttct
    12961 taagcgatgc cagcggaata cgcgttaaat cgctccaggc tcccagcaca cggttaaagt
    13021 cgatatcgac aatcacatta cctaaatcaa agatatagag catttttgcc tcctttcgcg
    13081 ccacaagaaa ttaactgtag cgggaaagga tgggtttggc tatgtggcaa ggtatgaagc
    13141 caggttaacg gtcacaaaaa tctacgcgtc ttcctgggga agaatgattt caatttggct
    13201 gtcttgtaag cgtgatttta gcgcagcggg cggcagctcg tcggtaaaga gcgcagtgac
    13261 ctgtgccacg ttaccaattt caaccgccgc cgaagcatga tacttagtgt gatcggcgac
    13321 cagcagaata tttctcgcgt gcgccatcat cgttttcacc acgttagctt cgtttacatc
    13381 aaactccatc aacgcgccat cgctctcaat cgccccaacg cttgttacca gataatcagc
    13441 acgaaaatca gccacaaagg acgccgctga agggccaatg atcccgctat tatgagagcg
    13501 caacgtaccg ccgggcacca tcacttcaaa gcgcgggttg tggtaaagaa tatgcgccac
    13561 acgcaggctg ttggtgatta tccgcaaatg attatggtta agtaacgccc gggcaacatg
    13621 ctcaacagtc gtaccaatgg tgataaatat tgttgaacca tcaggaatat agtctgccac
    13681 cgcttcggca atcgcttttt tttctcggtt tgcgaaacct cacgctgctc gaacgccgta
    13741 ttaacgacgc tggaagcccg acccgcgcca ccgtgatggc gcgtaatcag gccttgctcg
    13801 cttaatttac gaatatcccg gcggaccgtc tgcgtggaga catccagcag atttgccagc
    13861 tcatcaatat tcatataccc acgctcggcg atcagcatga ggagttggtc gtgccgcggg
    13921 ttaccggtta gttcggtaag gctcatgaaa attcctcgaa aaaccatcac cctggcattt
    13981 tatacaaaaa gtgacaaaaa agatcgggtt tgatcacagt cagggatccc ggctcgtcca
    14041 ccgggacgtg tgcattttaa cgccgctaca ccgctggcga agcggactga ctccgctaaa
    14101 tccccacttg ttgccagcgc caccgccaaa gcgccgtgaa aaacatcacc cgcacctgtg
    14161 gtatctacca catcaacttt gaaggccggt tgatgctgac gcccaccatt ttccagccag
    14221 tcgcagcctg cgctaccctg ggtcacatag acatgaccat ttgtgagcgt ttgtgcctgt
    14281 tttagcgcac tggccatctc tttcacgccc gttaagcgcg ccagacccgg ttctgaaaag
    14341 gccgcgtgat cgcttaatgc caccagctca ctgatatcct gcggcgtaat gtccccgtcc
    14401 agaacggtca tcacacccgc ctgacgggcc agggtgaagg cttttttaac gccgtcgtgc
    14461 cagcgtacat ctgccagcac aacatcccac tgagagaaat caatttcctc caaccactct
    14521 gcgtcaggca gcagatccgg gctggggtag ttaatgatta tccgctcgcc tttggtatcc
    14581 accatgatgg cggattgcga agatttcgcc tggttatacc gtttggtgta acgggtgtta
    14641 acgccccagg attccagctc tgctagcagg ctgttgcccg cgtcgtcatc acctacgcga
    14701 ccaataaaat cgacctgcgc ccccagcctt gccgccgcaa ccgctgccgt cgccgctggc
    14761 ccgccaccaa cttccgtata atttctcgcc acgtatttac cgctctccgt cggtaaccct
    14821 tccacgtaat agatgcgatc catcacggtt atacctacac aagcaacacg aatcatggtc
    14881 attccttaaa cattttggga tgcgttcatt ttcatttcac caattgttta aaaagtgacg
    14941 gctgtcaatt ttttgacgat aaattacaaa tgcgatcaaa aacagacaag caaagcatgt
    15001 cataaatgac aaaaagtgac atcactgtat tcaggagagg gttatggcag caatcgcgtt
    15061 tatcggttta ggacaaatgg gttcgccaat ggcgagcaat ttattgcagc aagggcatca
    15121 acttcgcgtc tttgatgtga acgccgaagc tgtgcggcat ctggtagaca aaggtgcgac
    15181 tcccgccgcc aacccggtgc aggccgctaa agatgccgaa tttatcatta ccatgttgcc
    15241 gaatggcgat ctggtgcgca acgtgttgtt cggtgaaaac ggcgtttgcg aaggcttatc
    15301 taccgatgcg ctggtcattg atatgtccac catccatccg ctgcaaaccg ataaattgat
    15361 tgccgatatg caagccaaag gcttcagcat gatggatgtt ccggtaggcc gtacttctgc
    15421 aaatgccatt accggtactc tgttactgct ggctggcggc accgctgaac aagttgaacg
    15481 tgccacaccg atcctgatgg cgatgggcag tgagttgatc aacgctggcg gtccgggcat
    15541 ggggatccgc gttaagctca tcaacaacta catgagcatc gcgctcaatg cgctttcggc
    15601 agaagccgcc gttttgtgcg aagccctgaa tcttcccttc gatgttgccg tcaaagtgat
    15661 gagcggtacc gccgccggta aaggtcactt caccacttcc tggccaaaca aagtcctcag
    15721 cggagatctt tctcccgcct tcatgatcga tcttgcccat aaggatcttg gcatcgccct
    15781 tgatgtcgcc aaccagctgc atgtgccaat gccgctgggg gccgcctcac gggaggttta
    15841 tagccaggcg cgcgcatcgg gcggttttgc catgctggcg gtcgatcagc gcgaagccat
    15901 gcgcatgatg tttgccgcgg ctggggcacc cgctccggta gccgatagcg ttttaactga
    15961 tttcaaagtt aacgctgcaa aggccctctc gccttatgcc tcggcgattc tggtagatca
    16021 acaattctgc taccgccagg tggttgagca aaacgcgatt gccaaaagtt gcgccatgat
    16081 tgtcgccgcc gatgagttca ttcctggcaa cggtattccg gtcgatagcg tggttattga
    16141 ccgcaaaatc aatccgctac agatcaaaca agacggtggc aaagccttaa aactgctggt
    16201 gctgtggcgt agtgatgaag atgcgcagca acgtctggat atggtgaaag agttcaacgg
    16261 actgtgccac tcacacggtc tggtaagcat cattgagcca gtcgtccgcc caccgcgtcg
    16321 tggcgataaa ttcgatcgcg aacaagcggt catcgatgcc gccaaagagc tgggcgacag
    16381 tggcgctgac ctctacaaag ttgaaatgcc gctttatggc aaaggtccgc aacaagagct
    16441 tctctgtgct tcacaacgtc tgaatgacca tatcaatatg ccatgggtga tcctctcttc
    16501 cagtgtcgac gaaaaactgt tcccgcgtgc cgtacgcgtg gcaatgacgg cgggcgcatc
    16561 gggattcctg gcaggccgtg cagtctgggc atcggtcgtc ggtttaccag acaacgagct
    16621 gatgctccgt gacgtttgcg caccgaaatt acaacaactt ggcgatatcg tcgacgaaat
    16681 gatggctaaa cgccgctaag atgaggataa aaggaatgaa atggtttaac accctaagcc
    16741 acaaccgttg gctggaacag gaaaccgacc gcatctttga ttttggtaaa aattccgtag
    16801 tgccgactgg ttttggctgg ttaggcaata aagggcaaat caaagaagag atgggcaccc
    16861 atctgtggat cacagctcgt atgttgcacg tttattccgt tgctgcggcg atgggtcgac
    16921 ctggcgctta cgcgttggtt gatcacggta ttaaagccat gaacggcgca ctgcgtgata
    16981 aaaaatatgg cggctggtat gcctgcgtga atgacgaggg cgtggtggat gcctccaaac
    17041 agggctatca acacttcttt gctctgctgg gtgccgccag cgccgtcaca acgggtcacc
    17101 cggaagcgcg caagctgctc gattacacca ttgagattat cgagaaatat ttctggagcg
    17161 aagaagagca gatgtgcctg gaatcctggg acgaagcctt cagcaaaacc gaagagtacc
    17221 gcggcggcaa tgccaatatg cacgcggtgg aagctttctt gattgtttat gacgtcactc
    17281 acgacaaaaa atggctggat cgcgcgattc gcgtggcttc cgtgattatc cacgacgtcg
    17341 ccagaaataa tcattatcgc gttaacgaac acttcgatac ccagtgggat ccgctgccgg
    17401 attacaacaa agataacccg gcgcatcgct tccgcgcgtt cggaggtaca ccaggccact
    17461 ggatcgaatg gggccgttta atgctgcaca tccatgctgc cctggaagcc cgttgcgaac
    17521 aaccaccagc atggctgcta gaagatgcca aaggtctgtt taacgccacc gtgcgcgatg
    17581 cctgggcacc cgatggcgcg gacgggattg tttataccgt tgactgggaa ggaaaaccgg
    17641 tggtccgcga acgtgtacgt tggcctatcg tcgaagcaat gggtacggcc tacgcgctct
    17701 acaccgtcac tggcgatcgc cagtatgaaa cctggtatca aacatggtgg gagtactgca
    17761 ttaagtacct gatggactat gaaaatggtt cctggtggca ggagctggat gcggacaata
    17821 aggtcatcac caaagtctgg gacggcaaac aggatattta tcacctgctg cattgcctgg
    17881 tgatcccgcg tatcccgtta gcgcccggca tggctccagc ggttgcagcg ggtctgctgg
    17941 atattaatgc gaaataactg atctgaaaat agggtgcggg ttttcccctc accctaaccc
    18001 tctccccaaa ggggcgaggg gaccgtccgg tgcagttttt gggtttcaca tcagcctcac
    18061 gggaggctgt atgtcgttaa ttaaggtgcc ttgtatgcaa atcaccaata tgcattgcag
    18121 tggacagacc gtttcacttg ccgcggggga ttatcacgcg accattgtga ccgtcggtgc
    18181 tggtctggct gaattaacct ttcagggttg tcatttagtc atcccgcaca aacccgaaga
    18241 gatgccgctg gcgcatctgg gtaaagtgct tattccgtgg ccgaaccgga tcgccaacgg
    18301 ctgttaccgt tatcaggggc aggaatatca gttacccatt aatgaacata gttcaaaagc
    18361 cgctattcac gggctgcttg cctggcgaga ctggcaaatt agtgaattaa ccaccaccag
    18421 cgttaccctg acagcttttc tgccgcccag ttacggctat ccgtttatgc tggcttcaca
    18481 agttgtttat tcgctcaacg cacacacggg tctttccgtt gagatcgcaa gccagaatat
    18541 cggcactgtc gcagcgcctt atggcgtggg tattcatcct tatttaacct gcaacttaac
    18601 atctgttgat gaatatctgt tccagcttcc ggcaaaccag gtttatgccg tcgatgaaca
    18661 cgtaaatccg acaacgcttc accacgttga tgagctggat ttaaatttca cgcaggcgaa
    18721 aaaaatagcc gcgacaaaaa tcgaccacac ctttaaaacc gctaatgatt tgtgggaaat
    18781 gaccattacc catccacagc aggcgctttc ggtaagcctt tgttccgatc aaccctgggt
    18841 gcaggtttat agcggtgaaa aattacagcg tcagggactg gcagttgaac ctatgagttg
    18901 cccgcctaac gcctttaatt caggtatcga cttattatta ctggaaccag gaaaaccgca
    18961 ccgcctgttt tttaatattt acggacagag aaaataactt gtattgatta gcagtcaaaa
    19021 tcattgaaga acatttcact cgctaaaggc aacattatgt atcttcaatt tattaagtta
    19081 aaaacagctc agcttctgta aagtttttat atatcagttt tatgagaatc attttacata
    19141 agcctgtaat aacaggaagg caacaatctc gttctcaact ctatggagat tagttatgga
    19201 tacgccacgt ccacagttat tagattttca atttcatcag aataacgaca gttttaccct
    19261 acattttcaa caacgtctta ttttaaccca tagcaaagat aatccttgtt tatggattgg
    19321 ctcaggtata gcggatatcg atatgttccg cggtaatttc agcattaaag ataaactaca
    19381 ggagaaaatt gcgcttaccg acgccatcgt cagccagtca ccggatggtt ggttaattca
    19441 tttcagccgt ggttctgaca ttagcgccac gctgaatatc tctgccgacg atcagggccg
    19501 tttattgctg gaactacaaa acgacaacct taaccacaac cgtatctggc tgcgccttgc
    19561 cgctcaacca gaggaccata tctacggctg cggcgaacag ttttcctact tcgatctgcg
    19621 cggcaaaccg ttcccgctat ggaccagtga acaaggcgtt ggtcgcaaca aacaaaccta
    19681 tgtcacctgg caggccgact gcaaagaaaa tgcgggcggc gactattact ggactttctt
    19741 cccacagcct acgtttgtca gcacgcagaa gtattactgc catgttgata acagttgcta
    19801 catgaacttc gactttagcg ccccggaata ccatgaactg gcgctgtggg aagacaaagc
    19861 tacgctacgt tttgaatgtg ctgacacata catctccctg ctggaaaaat taactgccct
    19921 gctgggacgc cagccagaac tgcccgactg gatttatgac ggggtaacgc tcggcattca
    19981 tggcgggacg gaagtgtgcc agaagaaact ggacaccatg cgtaacgcgg gcgtgaaggt
    20041 taacggcatc tgggcgcagg actggtctgg gatccgtata acctcttttg gcaagcgcgt
    20101 gatgtggaac tggaagtgga acagcgaaaa ctacccgcaa ctggattcac gcattaagca
    20161 gtggaatcag gagggcgtgc agttcctggc ctatatcaac ccgtatgttg ccagcgataa
    20221 agatctctgc gaagaagcgg cacaacacgg ctatctagca aaagatgcct ctggcggtga
    20281 ctatctggtg gagtttggcg agttttacgg cggcgttgtc gatctcacta atccagaagc
    20341 ctacgcctgg ttcaaggaag tgatcaaaaa gaacatgatt gaactcggct gcggcggctg
    20401 gatggctgac ttcggcgagt atctgcccac cgacacgtac ctgcataacg gcgtcagtgc
    20461 cgaaattatg cataacgcct agcctgcgct gtgggcgaag tgtaactacg aagcccttga
    20521 agaaacaggc aagctcggcg agatcctctt ctttatgcgc gccggttcta ccggtagcca
    20581 gaaatactcc accatgatgt gggcgggcga ccagaacgtg gactggagtc tcgacgatgg
    20641 cctggcgtcc gtagtcccgg cggcgctgtc gctggcaatg accggacatg gtctgcatca
    20701 cagcgacatt ggcggttaca ccaccctgtt tgagatgaag cgcagcaaag agctgctgct
    20761 gcgctggtgc gatttcagcg ccttcacgcc gatgatgcgc acccacgaag gtaaccgtcc
    20821 tggcgacaac tggcagtttg acggcgatgc ggaaactatc gcccattttg cccgtatgac
    20881 taccgttttc accaccctga aaccttatct gaaagaggcc gtcgcgctga atgcgaagtc
    20941 cggtctgccg gtcatgcgcc cgctgttcct gcattacgaa gacgatgcac atacctattc
    21001 cctgaaatat cagtacctgt taggccgcga cattctggtc gctccggtac acgaagaagg
    21061 tcgcagcgac tggacgctct atctgccgga ggataactgg gtacatgcct ggacgggtga
    21121 agcgttccgg ggcggggaag ttaccgttaa tgcgcccatc ggcaagccgc cggtctttta
    21181 tcgcgccgat agcgaatggg cggcactgtt cgcgtcgtta aacagcatct aatcacatcc
    21241 gcccgtgggg aaactcatgg gcaaagggag aacatcgatg agtcacatca caacggaaga
    21301 tccagcaact ttacgcctgc cctttaaaga gaaactctct tacggtattg gcgacctggc
    21361 ctctaacatc ctgctggata tcggtacgct ttatcttttg aagttttata ccgacgttct
    21421 ggggctgcca ggcacctatg gcggcattat ctttttgatt tcaaaattct ttactgcgtt
    21481 taccgatatg ggtaccggca ttatgttgga ttcccgacgc aagatcggtc caaaaggtaa
    21541 gttccgtcct tttattctgt atgcgtcatt cccggtcacc ttactggcga tcgccaactt
    21601 tgtcggcaca ccgtttgatg tcaccggtaa aacggtgatg gccactattc tgtttatgct
    21661 ttacggactg tttttcagca tgatgaactg ctcctacggc gcaatggtcc cggcaatcac
    21721 caaaaacccc aacgaacgcg cctcactggc ggcatggcgt cagggcggcg ctacgctggg
    21781 cctgctgctg tgcacggtgg gattcgtgcc agttatgaat cttatcgaag gtaatcagaa
    21841 acttggctat atcttcgccg ccacgctgtt ttcactgttc ggcctgctgt ttatgtggat
    21901 ctgctactca ggcgtgaaag agcgttatgt cgaaacccaa ccaaccaatc cggcgcaaaa
    21961 gcctggcctg ttgcaatctt tccgcgcaat tgccggtaac cgcccactgt tcattctgtg
    22021 tattgccaac ctctgcacct taggggcgtt taacgtcaag ctcgccattc aggtctatta
    22081 cacccagtac gtacttaacg atcccatcct gttgtcgtat atgggatttt tcagcatggg
    22141 ctgtattttc atcggcgtgt tcctgatgcc tggcgcagtc agacgttttg gtaagaagaa
    22201 ggtctatatc ggcggcctgc tgatttgggt gctgggcgat ctgctcaact atttcttcgg
    22261 cggcggctcg gtcagctttg tggcgttctc ctgcctggcg ttcttcggct cagcgtttgt
    22321 taacagcctg aactgggcgc tggtttccga caccgtcgag tacggcgagt ggcgcaccgg
    22381 cgtgcgttcg gaaggcacgg tctacaccgg cttcaccttc tttcgcaaag tgtctcaggc
    22441 gctggctggt ttcttccccg gctggatgct gacgcaaatt ggctatgtgc cgaacgtcgc
    22501 acaggctgac cacaccattg aagggttacg ccagttgatc ttcatctacc caagcgcact
    22561 ggcggtagtc accattgtgg cgatgggttg cttctacagc ctgaacgaga agatgtatgt
    22621 ccgcattgtg gaagagatag aagcccgtaa acgcacggcg taataataat aaacgacgcc
    22681 ctgcggggcg ttataaggag tgattatgtc tgaccataat ccactgacat taaaactgaa
    22741 tctgcgggaa aaaatcgcct atggtatggg cgacgtcggt tcgaatttaa tgctctgcat
    22801 cggtactctg tatctcctca aactttatac cgatgaatta gggatgcctg cttactatgg
    22861 cgggattatc tttctggtcg cgaagttttt taccgcgttt accgatatgc tcaccggatt
    22921 tttactcgac tcgcggaaaa atattgggcc taaaggcaaa tttcgtccct ttattttata
    22981 tgcagcagtt ccggcggcgt taattgcaac gctccagttt atcgccacca ccttttgttt
    23041 gccggttaaa acgacgattg ccaccgcgct gtttatgatg tttggacttt catacagtct
    23101 gatgaactgc tcgtatggtg cgatgatccc ggcaattacc aaaaacccga atgagcgcgc
    23161 gcagctcgcg gcttaccgcc agggtggtgc aaccataggg ctattgattt gtaccgtggc
    23221 gtttattccg ttgcagtcgc ttttttctga ctcaaccgtc ggttatgcct gtgcggcact
    23281 tatgttctcc attggcggct ttatttttat gatgctgtgc tacagaggcg tcaaagagca
    23341 ttatgtggac acagcgccaa ccggacataa agccagtatt ctcaaatctt tttgcgcgat
    23401 atttcgtaat ccaccattgc tggttttatg cattgctaac ctgtgtactc tggcggcatt
    23461 taatatcaaa ctggcgattc aggtctatta cacccagtat gtgctgaatg atattaattt
    23521 attgtcgtgg atgggattct tcagtatggg atgcatcctc gtcggcgtat tactggtgcc
    23581 agtaactgta aaatgttttg gtaaaaaaca ggtttattta gctggcatgg tgctatgggc
    23641 ggtgggtgat atactgaatt atttctgggg aagtaactct ttcactttcg tcatgttctc
    23701 ttgtgtcgcc ttttttggca cggcctttgt aaacagcctg aactgggcac tggtaccaga
    23761 taccgtagat tacggtgaat ggaaaaccgg tattcgtgcc gaaggttctg tttataccgg
    23821 ttataccttc tttcgtaaaa tttctgccgc acttgctggc ttcttgccag gcattatgct
    23881 gacgcaaatt ggttatgttc ccaacatcgc gcaaagcgat gcgacattac agggtttgcg
    23941 tcagctcatt tttatctggc cttgtgcact ggcaattatt gctgcattga cgatgggatt
    24001 cttttacaca ctcaatgaaa aacggtttgc attgattatt gaggaaatca accaacgaaa
    24061 aaataaagag atggcgacag aaaaaaaaac ggcttccgta acgttataaa tgtcaatagc
    24121 cgctatttcc atcctggtgg gtggcggcct ccctacgttt aaaaaatgga cttattatga
    24181 aaaagattaa tgcaataatt ttattatctt ctctgacctc tgcctcggta tttgctggcg
    24241 catacgtcga aaacagggag gcatataatc ttgcctccga tcagggcgag gttatgctac
    24301 gtgtgggtta taacttcgat atgggcgcgg gtattatgtt aaccaatacc tacacctttc
    24361 agcgagaaga tgaactaaaa catggctata atgaaattga aggttggtat cctttattca
    24421 aaccaaccga taaattaacc atccagcccg gtggcttaat
//
LOCUS       DAJTNM010000051        24189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTNM010000051 DAJTNM010000000
VERSION     DAJTNM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.051"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(22..594)
                     /locus_tag="OO172_002153"
     CDS             complement(22..594)
                     /locus_tag="OO172_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5512336.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OO172_002154"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OO172_002154"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5512337.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO172_002155"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO172_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5512338.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO172_002156"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO172_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5512339.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OO172_002157"
     CDS             complement(3556..5337)
                     /locus_tag="OO172_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5512340.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="OO172_002158"
     CDS             complement(5330..7102)
                     /locus_tag="OO172_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5512341.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO172_002159"
                     /pseudo
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO172_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO172_002160"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO172_002160"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5512342.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10361
                     /locus_tag="OO172_002161"
                     /pseudo
     CDS             8662..10361
                     /locus_tag="OO172_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10426..11121
                     /gene="queC"
                     /locus_tag="OO172_002162"
     CDS             10426..11121
                     /gene="queC"
                     /locus_tag="OO172_002162"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5512343.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11173..11571)
                     /gene="fadM"
                     /locus_tag="OO172_002163"
     CDS             complement(11173..11571)
                     /gene="fadM"
                     /locus_tag="OO172_002163"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5512344.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11665..12036)
                     /locus_tag="OO172_002164"
     CDS             complement(11665..12036)
                     /locus_tag="OO172_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5512345.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNLLVERNLAVLTL"
     gene            complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="OO172_002165"
     CDS             complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="OO172_002165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5512346.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="OO172_002166"
     CDS             complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="OO172_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5512347.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14731..17085)
                     /gene="lon"
                     /locus_tag="OO172_002167"
     CDS             complement(14731..17085)
                     /gene="lon"
                     /locus_tag="OO172_002167"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5512348.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="OO172_002168"
     CDS             complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="OO172_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5512349.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="OO172_002169"
     CDS             complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="OO172_002169"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5512350.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19543..20841)
                     /gene="tig"
                     /locus_tag="OO172_002170"
     CDS             complement(19543..20841)
                     /gene="tig"
                     /locus_tag="OO172_002170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5512351.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="OO172_002171"
     CDS             complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="OO172_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5512352.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21807..22385
                     /locus_tag="OO172_002172"
     CDS             21807..22385
                     /locus_tag="OO172_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5512353.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22429..23904
                     /gene="ampG"
                     /locus_tag="OO172_002173"
     CDS             22429..23904
                     /gene="ampG"
                     /locus_tag="OO172_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5512354.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6661 c         5837 g         6024 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattg cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccaga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcccacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataattgtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctca gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcaa gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gttgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gagatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact acgcctactg ccgcccgatt aaacagcagc gactgagcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccagccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgagttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggatcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctagacgcgc atcagataat cgtattcacc agccatgcgc cagaacccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt
     8761 ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg cacaggaggc gggatggctg
     8821 gagtggcagg cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgccg
     8881 gaatcgctac gcaatgcgat gatggaacag gcactggaaa ccggaaagca gcaagatgtg
     8941 ctggagctgg cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc
     9001 ggacaatggc aaacgataca cccacgttgc gtattcccta ctatcgcccg ctcgaaccgc
     9061 tacaaccagg ctttttgccc ggccgtgccg agcagcatct cgccgggcag atattttccg
     9121 gcctgacccg cttcgataat aatactcagc gcccgattgg cgatttagcg catcactggg
     9181 aaacctctac tgacgggtta cgctgggact tttatcttcg ttcaacccta cactggcata
     9241 acggcgatgc agtaaaagcc tcacacttac accagcgatt attgatgctg ttacaactgc
     9301 cagcactgga tcaattattt attagcgtga agcgtattga agtcacccat ccgcagtgtc
     9361 tgaccttctt tttacatcgc cccgattact ggcttgcgca ccggctggcg agctattgca
     9421 gccatctggc gcatccgcaa ttcccactga tcggcactgg tccttttcgc ttaacacaat
     9481 tcacagcgga gctggtgcgc ctggagagcc atgattatta ccatttgcgt catccgctgc
     9541 ttaaagcggt tgagtactgg ataactccgc cgcttttcga aaaagatttg ggaaccagtt
     9601 gtcggcatcc cgtgcaaatc accatcggca aaccggagga gctgcaacgg gtcagccagg
     9661 tcagtagtgg catcagttta ggtttttgct atttaacgtt gcgcaaaagt ccccgactct
     9721 ccctctggca ggcgcgaaaa gtgatctcca ttattcatca atccggttta ttacaaacgt
     9781 tagaagtcgg agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc
     9841 cgcactggca agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcacc
     9901 taccgataga acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct
     9961 gtgaactcac aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac
    10021 acgcagacct catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc
    10081 aatggctacg ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc
    10141 tgcaatcgac gctggacgcg gtgcaagtaa tgcctgatga ggaaaaccga tttaatgccc
    10201 tgaaagcggt ttttagccag ttaatggcag acgcgacgct gacgccgctg ttcaactatc
    10261 actatcgcat tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt
    10321 ttgaatttac cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat
    10381 cgcaggcgtt accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc
    10441 gttgtgttca gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat
    10501 gatgaagtcc attgcgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg
    10561 gcacgcgaac tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg
    10621 ctcaacgagc tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa
    10681 cctgaagccg atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg
    10741 ctggcggcaa tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa
    10801 acggatttct ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc
    10861 gtcagtttgg gcatggcgaa agatattcgt tttgaaacgc cgctgatgtg gattgataaa
    10921 gcggaaacct gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg
    10981 ttgacctgct ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta
    11041 cgcgccaacg gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag
    11101 cagaaaaccg ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc
    11161 accacaaaac gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc
    11221 taatgctttc tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac
    11281 ctgcccttcc ggctccagtg taatgacctg gcttaagatg ccgcttttac cgtttaattg
    11341 ctgcaactga ctggtaatag ttaacaggtc gcttaatacc gctgggcgac gatagttaat
    11401 attgatattg accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct
    11461 attttccaac ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac
    11521 gtgctggtaa acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata
    11581 accttacgtt gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca
    11641 aaaaattgcc actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag
    11701 caaattgccc atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc
    11761 ttcgcgataa ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc
    11821 tagctcttcc gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt
    11881 cgccggtact gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt
    11941 cggtttcgcc acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga
    12001 cagggtaatg agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac
    12061 agtggaatca ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa
    12121 aaggccgcga aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct
    12181 tcaggattat tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt
    12241 actcatcaga gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc
    12301 ttttttctga tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac
    12361 attaccttgc atatcagtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag
    12421 tgcaaacgcc gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc
    12481 aaatttcaga ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag
    12541 cagtttctca gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt
    12601 aacttgttcc tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgacgcg
    12661 cagcacgaat gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccgacgcgcc
    12721 gttttcacct accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc
    12781 ttccggcagg ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg
    12841 ctctgcaccg gccagagact cggtttcgtt gcttgccgca tcgctcactt tctgctgcag
    12901 cgcatagtac gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac
    12961 ttcgtctaac gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc
    13021 gaccgaagat ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag
    13081 ttcgtcgggg atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat
    13141 aatatcggca gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc
    13201 aagtaccgct ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg
    13261 cggctgggtg aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac
    13321 cggttgctgc atcgttgcgg caaccagctt gatgtaactc acgcggaatt gttccggcgt
    13381 catgaaattg tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg
    13441 cttcgccgcc agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac
    13501 cagtgccgcc agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt
    13561 aatcagctgt tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat
    13621 ccccatctgg ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa
    13681 ggctggggtc gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc
    13741 acgagcgtac tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag
    13801 ggttttcata tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg
    13861 catgcgatta cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc
    13921 attcactttt gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa
    13981 tatgaacgac acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt
    14041 gcgtaagctg tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc
    14101 aacgtatcga tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac
    14161 aagacacaga aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa
    14221 cttcgtcaca tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct
    14281 gcacggaagc tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg
    14341 cggccagtac gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca
    14401 tcatcccctt ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca
    14461 gccgcagctt tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc
    14521 actcttctct tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc
    14581 agcagttata tcaggccagc catcccctta caacacccct taataagggc aagcccgatc
    14641 cgctatctaa cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct
    14701 gtttttatta gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga
    14761 cggttcattt tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc
    14821 cagatcggca attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat
    14881 tagcactgtt ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat
    14941 cggcagtacc tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg
    15001 gttaccggtc aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc
    15061 tttcggcgtc gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg
    15121 gttgatcccc agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc
    15181 ctgcatcact tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc
    15241 ggtttcaatg gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctgacc
    15301 gacacggttt tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg
    15361 caggttatcg ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac
    15421 cgctttgcgg cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt
    15481 gtagtaacga ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag
    15541 tgcattacgt tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc
    15601 ttcggtataa ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt
    15661 catggagttc gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta
    15721 gtggtcgctg aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg
    15781 atcgcctcgc atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt
    15841 cacgcccact ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg
    15901 gcggtgacca cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt
    15961 acgcccggtg gctttggcaa tggactgacc aagagaggtt ttacctaccc ccggcggccc
    16021 taccaggcag aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc
    16081 aaggatgcga tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc
    16141 ctgacgcagg tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc
    16201 gatataacca cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg
    16261 caactctgct tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg
    16321 cttcagggct tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc
    16381 tttcatttgc tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg
    16441 gttgcgaatg cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag
    16501 atattccaga cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt
    16561 cagcggcata tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag
    16621 cgacgtcagc acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct
    16681 gattgcagta cgcaccagca cttcctgttc ccgctcatca atggtcggcg actccagata
    16741 ctccgccttc gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg
    16801 taacccctcg accagcactt tgacggtgcc gtcaggcagt ttcagcatct gcaatataga
    16861 ggccacggtc ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt
    16921 ctgcgcgacc agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga
    16981 tttttcccgc ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag
    17041 cggcaatacg gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag
    17101 tttaatttcc gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt
    17161 acgtcagtat atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa
    17221 ggggagataa aatcccccct ttttgtttaa ctaattgtat gggaatggtt aattattcac
    17281 cagatgcctg ttgcgcttcc ggcttaccat aaatcagcaa cggtttgctt tgaccatcaa
    17341 ttaccgactc gtcaataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg
    17401 tatcgagcag tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca
    17461 tcgctttctt agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca
    17521 gattaaacag cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa
    17581 tcagagcttc ttcgctcagt tcattcaacg ttgctacaac cggcagacga ccaatgaact
    17641 cagggataag accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt
    17701 cgcttgcttt gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt
    17761 ctacacggtg ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga
    17821 tcttagaggt atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg
    17881 gaacagcagc taccgtacct tcgatcagtt tcaacagtgc ctgctgtact ccttcaccgg
    17941 aaacgtctcg ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga
    18001 tgtagacaat cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct
    18061 gaatgatgtt ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg
    18121 ccatggtgaa cgggacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg
    18181 aaccggtcgg accgatcagc agaatgttac ttttgcccaa ctcgacacca ttgctggtat
    18241 cgccgttgcg cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct
    18301 gttcctggcc gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg
    18361 cactgcgttc acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata
    18421 aatcaacaca ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat
    18481 gctggctttt gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat
    18541 ctgtcatggg tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca
    18601 ttctcaggcg caagccgctt acagcgttgt gccgccctag ataagtatag cggcacagtt
    18661 gcgcctctgg catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc
    18721 ttcaggggcg gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg
    18781 acccgtatga agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgagc
    18841 atgaatttcg atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac
    18901 gcgcgaattc ggcaggcaaa aacgtttacc ttttgcccct gcggtcaaca agaaagcgcc
    18961 catcgaggcc gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt
    19021 gtcatagata gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat
    19081 atctttttct gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat
    19141 gtggtcttca acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat
    19201 atcaaaagag cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg
    19261 gggtgcaaag ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa
    19321 aaaaacctgc tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat
    19381 gggtacggca tcaccatatt tcaagcataa caatcttttg ttgttacgct aacaccgaaa
    19441 gaggcttttc gcacggctcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg
    19501 tgacgggcct ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat
    19561 cagctcgttg aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac
    19621 agcctgttct tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta
    19681 gaactcgata acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt
    19741 cacgcgctct tcgtcagctt tcagctcgtt ggtgcggata acttcgccca gcagcaggcc
    19801 aacaactacg cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg
    19861 tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc
    19921 gatcagcgca gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga
    19981 cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc
    20041 gcgcagacct tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag
    20101 ttccggcagt tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc
    20161 tttcaggttt tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc
    20221 agctttgtgg cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc
    20281 cagtacgaaa tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa
    20341 gtcgatggtt acgcggtctt ctgcttcaac agcgccgtct ttttctttcc aggtcgcctg
    20401 ctgtttacgc agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg
    20461 tttttcaact tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc
    20521 tacagagtaa gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc
    20581 agccggattg attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc
    20641 cagaacgtcc tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt
    20701 gcctttgcgg aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac
    20761 agcggtctcg atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt
    20821 ggtttcaact gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt
    20881 accccgcaag cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat
    20941 tccctgttac cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc
    21001 acttttatga gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg
    21061 caattttcgt ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc
    21121 aggccgtttt tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc
    21181 gtttttacgc gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca
    21241 acccttccca ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga
    21301 gttcctccgc taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa
    21361 aacgatcgct gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac
    21421 gatagctttc atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt
    21481 ctatccgctc acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc
    21541 cctttaaata ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt
    21601 tcgtcctggt ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc
    21661 ctcgcatcag cgtttctttt accaggggtg attatttcgt caacatgatg aatttacatg
    21721 ccttacccac ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac
    21781 gcttaagagt ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc
    21841 tgtttatgct tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta
    21901 cactgccaca gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc
    21961 agcgtacaga tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg
    22021 cctcccgcga tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg
    22081 gttacatggt tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg
    22141 ctgacgtgtc ccagggcaac gtgcgctaca acatcgctac caaagcggat atcgccatca
    22201 tcgctaccgc gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg
    22261 aaggtgcgtt ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg
    22321 ataccatcgc tgatatgtct caggacacca gcattcacga attcatcaag cagaacgcgc
    22381 gttaatttct gccctctggc ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa
    22441 tatttacgta tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc
    22501 gggctaccgc tcgccctgac atccggcacc ttacaggcct ggatgacggt cgaaaatatc
    22561 gatctcaaaa ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc
    22621 tggtcaccgc tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg
    22681 ctcgccacgc aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaaccaggc
    22741 acccaactcc gctggatggc ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag
    22801 gatattgtct tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc
    22861 gcggcaatca gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cctagccctg
    22921 tggctggcag ataaatggtt gggctggcag ggcatgtact ggttgatggc ggcgctgttg
    22981 atcccctgta ttatcgcgac gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc
    23041 aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc
    23101 tggcttattt tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg
    23161 acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac
    23221 aaaacgcttg gcttactggc gaccattgtt ggcgcattgt acggtgggat tttgatgcag
    23281 cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct
    23341 ggttactggc tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt
    23401 ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg
    23461 tgtaataagt cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtaggg
    23521 cgagtttatg tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc
    23581 tatctattct ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag
    23641 acgcttgaat atacacgagt aaatgacaac tttatctccc gtaccgaata tccggcaggt
    23701 tatgcctttg ccatgtggac actggcggcg ggcgtcagcc tgttggccgt gtggttactt
    23761 ctgttgacga tggacgcgct ggatttgacg cacttctctt tcctgcctgc tctgctggaa
    23821 gtcggggttt tagtcgccct ttctggcgtc gtgcttggtg gtttgctgga ttatctggcg
    23881 ctacgaaaaa cgcatctgac gtaatctgta aatattattt agctgtagta atcactcgcc
    23941 gtaattatta cggctaaata aacatcagca tcgcttatta atatttgttc ttttgtgcgg
    24001 cttagcgttt ggaaattatt ggcgccattt ataaattcta tttttcttac acgattcagc
    24061 taatgagtct ttattttctc accacccagt tgtcactcta atgataatta tttgttaaat
    24121 aattgtttta tttcgcattg gttataccaa ttgcccgcca agactccttc tgcactcccc
    24181 ttttgttat
//
LOCUS       DAJTNM010000052        24118 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTNM010000052 DAJTNM010000000
VERSION     DAJTNM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.052"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..1272
                     /gene="glpK"
                     /locus_tag="OO172_002174"
     CDS             <1..1272
                     /gene="glpK"
                     /locus_tag="OO172_002174"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5512355.1"
                     /translation="ITNQRETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLED
                     YISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHV
                     TDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIP
                     ISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTG
                     EVNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLSAP
                     YWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGA
                     VANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREF
                     RPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            1502..2512
                     /gene="glpX"
                     /locus_tag="OO172_002175"
     CDS             1502..2512
                     /gene="glpX"
                     /locus_tag="OO172_002175"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5512356.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLNRKDPEMQVH
                     IL"
     gene            2609..3355
                     /gene="fpr"
                     /locus_tag="OO172_002176"
     CDS             2609..3355
                     /gene="fpr"
                     /locus_tag="OO172_002176"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5512357.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(3360..3788)
                     /gene="uspD"
                     /locus_tag="OO172_002177"
     CDS             complement(3360..3788)
                     /gene="uspD"
                     /locus_tag="OO172_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5512358.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLENKSDNKLYKLTKNIQCPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(3815..4114)
                     /locus_tag="OO172_002178"
     CDS             complement(3815..4114)
                     /locus_tag="OO172_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5512359.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(4326..4766)
                     /locus_tag="OO172_002179"
                     /pseudo
     CDS             complement(4326..4766)
                     /locus_tag="OO172_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            4867..5466
                     /locus_tag="OO172_002180"
     CDS             4867..5466
                     /locus_tag="OO172_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5512360.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            5574..6341
                     /gene="tpiA"
                     /locus_tag="OO172_002181"
     CDS             5574..6341
                     /gene="tpiA"
                     /locus_tag="OO172_002181"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5512361.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(6396..7151)
                     /gene="cdh"
                     /locus_tag="OO172_002182"
     CDS             complement(6396..7151)
                     /gene="cdh"
                     /locus_tag="OO172_002182"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5512362.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKSVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(7258..8247)
                     /gene="sbp"
                     /locus_tag="OO172_002183"
     CDS             complement(7258..8247)
                     /gene="sbp"
                     /locus_tag="OO172_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5512363.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(8567..9529)
                     /gene="pfkA"
                     /locus_tag="OO172_002184"
     CDS             complement(8567..9529)
                     /gene="pfkA"
                     /locus_tag="OO172_002184"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5512364.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(9710..10612)
                     /gene="fieF"
                     /locus_tag="OO172_002185"
     CDS             complement(9710..10612)
                     /gene="fieF"
                     /locus_tag="OO172_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5512365.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(10760..11260)
                     /gene="cpxP"
                     /locus_tag="OO172_002186"
     CDS             complement(10760..11260)
                     /gene="cpxP"
                     /locus_tag="OO172_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5512366.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            11410..12108
                     /gene="cpxR"
                     /locus_tag="OO172_002187"
     CDS             11410..12108
                     /gene="cpxR"
                     /locus_tag="OO172_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5512367.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            12105..13478
                     /gene="cpxA"
                     /locus_tag="OO172_002188"
     CDS             12105..13478
                     /gene="cpxA"
                     /locus_tag="OO172_002188"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5512368.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(13584..14258)
                     /gene="yiiM"
                     /locus_tag="OO172_002189"
     CDS             complement(13584..14258)
                     /gene="yiiM"
                     /locus_tag="OO172_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5512369.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(14407..15390)
                     /gene="kdgT"
                     /locus_tag="OO172_002190"
     CDS             complement(14407..15390)
                     /gene="kdgT"
                     /locus_tag="OO172_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5512370.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(15650..16270)
                     /gene="sodA"
                     /locus_tag="OO172_002191"
     CDS             complement(15650..16270)
                     /gene="sodA"
                     /locus_tag="OO172_002191"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5512371.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            16555..17589
                     /gene="rhaT"
                     /locus_tag="OO172_002192"
                     /pseudo
     CDS             16555..17589
                     /gene="rhaT"
                     /locus_tag="OO172_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(17586..18452)
                     /gene="rhaR"
                     /locus_tag="OO172_002193"
     CDS             complement(17586..18452)
                     /gene="rhaR"
                     /locus_tag="OO172_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5512372.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(18508..19344)
                     /gene="rhaS"
                     /locus_tag="OO172_002194"
     CDS             complement(18508..19344)
                     /gene="rhaS"
                     /locus_tag="OO172_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5512373.1"
                     /translation="MTVLRSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            19632..21101
                     /gene="rhaB"
                     /locus_tag="OO172_002195"
     CDS             19632..21101
                     /gene="rhaB"
                     /locus_tag="OO172_002195"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5512374.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            21098..22357
                     /gene="rhaA"
                     /locus_tag="OO172_002196"
     CDS             21098..22357
                     /gene="rhaA"
                     /locus_tag="OO172_002196"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5512375.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            22599..23423
                     /gene="rhaD"
                     /locus_tag="OO172_002197"
     CDS             22599..23423
                     /gene="rhaD"
                     /locus_tag="OO172_002197"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR5512376.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            23433..23747
                     /gene="rhaM"
                     /locus_tag="OO172_002198"
     CDS             23433..23747
                     /gene="rhaM"
                     /locus_tag="OO172_002198"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5512377.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            24048..>24118
                     /locus_tag="OO172_002199"
     CDS             24048..>24118
                     /locus_tag="OO172_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5512378.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLK"
BASE COUNT         5987 a         6297 c         6133 g         5701 t
ORIGIN      
        1 attacgaacc agcgtgaaac cactattgtc tgggaaaaag aaaccggcaa gcctatctat
       61 aacgccattg tctggcagtg ccgtcgtacc gcagaaatct gcgagcattt aaaacgtgac
      121 ggtttagaag attatatcag cagcaatacc ggtctggtga ttgacccgta cttttctggc
      181 accaaagtga agtggatcct cgaccatgtg gaaggctctc gcgagcgtgc acgtcgtggt
      241 gaattgctgt ttggtacggt tgatacgtgg cttatctgga aaatgactca gggccgtgtc
      301 catgtgaccg attacaccaa cgcctctcgt accatgttgt tcaacatcca taccctggac
      361 tgggacgaca aaatgctgga agtgctggat attccgcgcg agatgctgcc agaagtgcgt
      421 cgttcttccg aagtatacgg tcagactaac attggcggca aaggcggcac gcgtattcca
      481 atctccggga tcgccggtga ccagcaggcc gcgctgtttg gtcagttgtg cgtgaaagaa
      541 gggatggcga agaacaccta tggcactggc tgctttatgc tgatgaacac tggcgagaaa
      601 gcggtgaaat cagaaaacgg cctgctgacc accatcgcct gcggcccgac tggcgaagtg
      661 aactatgcgt tggaaggtgc ggtgtttatg gcaggcacat ccattcagtg gctgcgcgat
      721 gagatgaagt tgattaacga cgcctacgat tccgaatatt tcgccaccaa agtgcaaaac
      781 accaatggtg tgtatgtggt tccggcattt accgggctga gtgcgccgta ctgggacccg
      841 tatgcgcgcg gggcgatttt cggtctgact cgtggggtga acgctaacca cattatacgc
      901 gcgacgctgg agtctattgc ttatcagacg cgtgacgtgc tggaagcgat gcaggccgac
      961 tctggtatcc gtctgcacgc cctgcgcgtg gatggtggcg cagtagcaaa caatttcctg
     1021 atgcagttcc agtccgatat tctcggcacc cgcgttgagc gcccggaagt gcgcgaagtc
     1081 accgcattgg gtgcggccta tctcgcaggc ctggcggttg gcttctggca gaacctcgac
     1141 gagctgcaag agaaagcggt gattgagcgc gagttccgtc caggcatcga aaccactgag
     1201 cgtaattacc gttacgcagg ctggaaaaaa gcggtcaaac gcgcgatggc gtgggaagaa
     1261 cacgacgagt aatgtcaggc acctgaatcg attgcctgat gctgatcgcc tgatgcgacg
     1321 cttacgcgtc ttatcaggcc tacgagccaa tgccattttg tgtaggcggg ataaggcgtt
     1381 cacgccgcat ccggcatcac gcagactctg aaaccgtgcg ggggcaaccc cgcacacatc
     1441 aataatccca cccttcccct gtgctacact tcgcgccatt ccttactgct tagagtttgc
     1501 tatgagacga gaacttgcca tcgaattttc ccgcgtcacc gaatctgcgg cgctggctgg
     1561 ctacaaatgg ttaggacgcg gcgataaaaa caccgcggac ggcgcggcgg taaacgccat
     1621 gcgtattatg ctcaaccagg tcaacattga cggcaccatc gtcattggtg aaggtgaaat
     1681 cgacgaagca ccgatgctct acattggtga aaaagtcggt actggtcgcg gcgacgcggt
     1741 agatattgct gttgatccga ttgaaggcac gcgcatgacg gcgatgggcc aggctaacgc
     1801 gctggcggtg ctggcagtag gcgataaagg ctgcttcctc aatgcgccgg atatgtatat
     1861 ggagaagctg attgtcgggc cgggagccaa aggcaccatt gatctgaacc tgccgctggc
     1921 ggataacctg cgcaatgtag cggcggcgct cggcaaaccg ttgagcgaac tgacggtaac
     1981 gattctggct aaaccacgcc acgatgccgt tatcgctgaa atgcagcaac tcggcgtacg
     2041 cgtatttgct attccggacg gcgacgttgc ggcctcaatt ctcacctgta tgccagacag
     2101 cgaagttgac gtgctgtacg gtattggtgg cgcgccggaa ggcgtggttt ctgcggcggt
     2161 gatccgcgca ttagatggcg acatgaacgg tcgtctgctg gcgcgtcatg acgtcaaagg
     2221 cgacaacgaa gagaatcgtc gcattggcga gcaggagctg gcacgctgca aagcgatggg
     2281 catcgaagcc ggtaaagtat tgcgtctggg cgatatggcg cgcagcgata acgtcatctt
     2341 ctctgccacc ggtattacca aaggcgatct gctggaaggt attagccgca aaggcaatat
     2401 cgcgactacc gaaacgctgc tgatccgcgg caagtcacgc accattcgcc gcattcagtc
     2461 catccactat ctgaatcgca aagacccgga aatgcaagtg cacattctct gagtgatttg
     2521 atcgattgag ccttccagtc cttcgggact ggaatttttt tgttcggaga acgaagataa
     2581 ggcaagtcaa tcaaaacagg agaaaaaaat ggctgattgg gtaacaggca aagtcactaa
     2641 agtgcagaac tggaccgacg ccctgtttag tctcaccgtt cacgcccccg tgcttccgtt
     2701 taccgccggg caatttacca agcttggcct tgaaatcgac ggcgaacgcg tccagcgcgc
     2761 ctactcctat gttaactcgc ccgataatcc cgatctggag ttttacctgg tcaccgtccc
     2821 cgatggcaaa ttaagcccac gattggcggc actgaaaccg ggcgatgaag tgcaggtggt
     2881 tagcgaagca gctggcttct ttgttctgga tgaagtaccg gactgcgaaa cgctatggat
     2941 gctggcaacc ggtacagcga ttggccctta tttatcgatt ctgcaactag gcaaagattt
     3001 agatcgcttc aaaaatctgg tcctggttca cgccgcacgt tatgccgccg acttaagcta
     3061 tttgccactg atgcaagaac tggaaaaacg ctacgaaggc aaactgcgca ttcagacggt
     3121 ggtcagtcgg gaaacggcag cggggtcgct caccggacgg ataccggcat taattgaaag
     3181 tggggaactg gaaagcgcga ttggcctgcc gatgaataaa gaaaccagcc atgtgatgct
     3241 gtgcggcaat ccacagatgg tgcgcgatac acaacagttg ctgaaagaga cccggcagat
     3301 gacgaaacat ttacgtcgcc gaccgggcca tatgacagcg gagcattact ggtaagcggt
     3361 tacttatcga taaacggcac gatgagcaaa tccgcactca tcttattgat catcccgcga
     3421 tatgccggca tcaaacggtt gataaatgag tgatgatgac cacagacaag gaggtcgcac
     3481 tgctcttttt gcataatttc cagcagtgtt tccggcattt ctccgcgttc aatacgcagt
     3541 tttgtcttcg gacattgaat atttttcgtc agtttataca gcttgttatc cgacttattc
     3601 tccaacaatt gaagaatatc ttctgttgca gggaagtaga tacccggata caactcgctt
     3661 aagccatcat caatatgaat taacgtcagg tgagcgtcat tatgtctggc gagctccagg
     3721 gctttattca ccagtaaggc atcttcttca ttcccggaaa ttgccacgcc aatgtgttta
     3781 taagccatgt ttaactcctt ctaaagcaac tccatcaagc taacgaacgc agtgataact
     3841 caaaaataat catctcggcc tggcatgaga aagtgaaggc cgcatcaagc tcaacttgcc
     3901 cctccagatc cttaatctta taggccacgc tggccccttc atctgcagag gatgcagcgg
     3961 cggcaagctc tttcagtttg gtcataaaag actctgcttc ttcgcgggtg gcaaaaaagc
     4021 gcgaaaactt actggtacag ttgtcgttat caatcaccgt accgatatca atcgcacatc
     4081 ctttagtact gcatttatct acgacatctt tcatagggca cctctgtata ttcgcctctc
     4141 tgtcgattac gacttaagaa taatgcgccg ttaagggaag aacaaacaag taatgatctt
     4201 atggattacc attttttttg atattgctta aggtgaagag ttcagaagga ttttccgggt
     4261 attgtcattt gcatcgtgga aataacaatc ccggaaaaaa atagcagtaa gagatatatt
     4321 aaaggttaat tacttgattt attgtcggct ttatacttca catcctgagt atctttacca
     4381 tatttatttt cgccttgtgt gccaacaaac gcaccaagat cgataagcat catcaccaga
     4441 atcaacgtcg ggacaaaacg ccccaccgcc cattgccaga cacccggtaa aatcgctcag
     4501 ttacccgcca gcagcatcca cgccacaatc atcagaaatg cccatgcgcc ggaacgcccg
     4561 cgatcatgca agcgcttaac agttactgcc gctgttggcc agagcaagca cacaaggcaa
     4621 aacgccgcgg tctgaatatc gagtagattc ttacccgcca gtgagaaaag caccagcatg
     4681 cctgcgaacc acaggcctat ccaaatccag aaatcacggc gtccaatacg ccctttaaat
     4741 gagaataacc attgctgtat ggtcatgtaa gttccttgat ggttgtcttt tccaggattc
     4801 tacccttttg acaagggcga cagatatcgt tttaatcgga gccagtcata acaaaaggta
     4861 ctgtcaatga agccagggtg tacgctgttt tttctcttat gttctgcatt aaccgttaca
     4921 acaacggcgc atgcacaaac accagatacg gcaacgaccg cgccttatct gctggctgga
     4981 gcccctactt tcgatctctc catcagccag tttcgagaag actttaacag ccagaatccc
     5041 agcctgccac tgaacgaatt tcgtgccatc gacagcagtc ccgacaaagc caatctcact
     5101 cgtgctgcaa gtaaaattaa cgagaacttg tatgcttcta cagcgctgga gcgcggtacc
     5161 ttaaaaatca aaagcattca aatgacctgg ctacccatcc aggggccaga gcaaaaagca
     5221 gcgaaagcga aagctcagga atacatggca gcagtgatcc gcacactcac cccattaatg
     5281 accaaaacac aaagccagaa aaaactgcag tcgctactaa cggcggggaa aaacaaacgt
     5341 tattacaccg agacagaagg tgcactgcgt tatgttgtcg cggacaacgg cgaaaagggg
     5401 ctgaccttcg ctgttgaacc gattaagctg gcgctatctg aatcgcttga aggtttgaat
     5461 aaatgacaaa aagcaaagcc tttgtgccga tgaatctcta tactgtttca cagacctgct
     5521 gccctgcggg gcggccatct tcctttattc gcttataagc gtggagaatt aaaatgcgac
     5581 atcctttagt gatgggtaac tggaaactga acggcagccg ccacatggtt cacgagctgg
     5641 tttctaacct gcgtaaagag ctggcaggtg ttgctggctg tgcggttgca atcgcaccac
     5701 cggaaatgta catcgatatg gcgaagcgcg aagctgaagg cagccacatc atgctgggtg
     5761 cgcaaaacgt ggacctgaac ctgtccggcg cattcaccgg tgaaacttct gccgctatgc
     5821 tgaaagacat cggcgctcag tacatcatca tcggccactc tgaacgtcgt acttaccaca
     5881 aagagtctga cgaactgatt gcgaaaaaat tcgcggtgct gaaagagcag ggtctgactc
     5941 cggttctgtg catcggtgaa accgaagctg aaaacgaagc gggaaaaact gaagaagttt
     6001 gcgcacgtca gatcgacgcg gtactgaaaa ctcagggtgc tgcggcattc gaaggtgcgg
     6061 ttatcgctta tgaacctgta tgggcaatcg gtactggcaa atctgcaact ccggctcagg
     6121 cacaggctgt tcacaaattc atccgtgacc acatcgctaa agttgacgct aacatcgctg
     6181 aacaagtgat cattcagtac ggcggctctg taaacgcgtc taacgctgca gaactgtttg
     6241 ctcagccaga catcgacggc gcgctggttg gcggtgcttc tctgaaagct gacgctttcg
     6301 cagtgatcgt taaagctgca gaagcggcta aacaggctta agtctgacag gtgccggatt
     6361 tcatatccgg cacttacttt ccttaactcc tcgccttaac gcaaaatctc acactcatga
     6421 tcctggattt cctcggctga agcacggttc agcgtcagta gatttcgttg tgtcgccagc
     6481 aacacaaacg aattatcact ctgccgtacc atcgccagcc cgtagcttcc catatgttcc
     6541 cgcgcctcag gcacttcttc tgcaagcatc ataaatgggc tgcgttgtac cagttcgctt
     6601 tccgttaccc gacgcgccag gtattcatgc ccgcgcaaac cacctggcag tggcaaccag
     6661 cggctgctga tgttcgccag atttttatcc agttgttcgc gcacatcagg acgaatacaa
     6721 gagatatgaa tatgaaaatg gttttgcgta cgcccggtgc gggagttgat cgccaaagaa
     6781 accgcgcgat cgggaaccgg ctggccgtat tttttgctca taaaatcacg cgcctgccag
     6841 gccaaccaaa agaagttcgg cgttgaagga tcggtcaaca aaggactttc agtaccgtta
     6901 atacgatacg ttgacatcaa cagatattgc agtggaccat taagatcttt taaaaccacg
     6961 tatccggcat tgggtttgac ttccgcacat ggcgaaggat tttgattttg ctgctgattg
     7021 ggcaaacatt cctcaaggac actcttacgt aatgtatccg actcttcacc ggttaatttc
     7081 cagtaaccaa taccggcagc cacaacggcg ataactatca tcaccaaaaa aagaagaccc
     7141 gcttttttca tctttttttc cctgtacctc aaagagatgc aagggtaacg caaaatcgtg
     7201 acaaataaaa aacccggtcg cgagaaacca ccgggtttga taattatcct agggaaatca
     7261 gcgtttgctg atctgatcga acgtaccgcc gttagcaaaa tgctcttttt gcgctttcgt
     7321 ccagccgccg aactcttcat caatggtgaa taacttcagc tttggaaacg cattttcgta
     7381 ctttttcgct acttcagcgt cgcgcggacg gtagtagttt ttcgcggcaa tttcctgacc
     7441 ttctggcgag tagagatatt tcaggtaggc ttccgccacc tctttggtgc cttttttctc
     7501 gaccacttta tcgaccaccg acaccgttgg ctctgcgagg atagactcac tcggcgtgac
     7561 gatttcgaat ttatctttcc ccagttcatt cgctgccagc agagcttcgt tttcccaggc
     7621 gatcagtaca tcgccaatcc cgcgctcgac aaaggtgtta gtggagccgc gcgcgccaga
     7681 atccagaact tcgacgtttt tatacagtgc ccgaacaaaa tcctgtgctt ttgcctgatc
     7741 gttgttgttg tgatgcagcg cgtagcccca ggcagccagg tagttccagc gtgcgccacc
     7801 agagcttttc ggattaggcg tgatcaccga aacacccggt ttaatcagat cgttccagtc
     7861 atggatctgc ttcggattgc ccttacgcac caggaaaaca atggtggaag tgtacggtgc
     7921 ggagttatcc ggcagacgtt tgatccactc tttatcaatc cgcccgcgtt ccgcaatcgc
     7981 gtccacgtca taggccagag ccagcgtgac aacatcagct tcaataccgt tgattaccga
     8041 cgtcgcttgt ttacctgaac caccgtgtga ctgacgaatc accacgttat caccagtttg
     8101 ctgtttccag tgggcgctga atgccttgtt gtactgttcg tacaattcgc gcgttggatc
     8161 atatgaaacg ttaagaagct gaatatcctt tgccataacg ctggttgccg ccagcaaaaa
     8221 tgttaaccct acgccccact tgttcatcgc ccgactctct tatgttgtgt tgtgatgagc
     8281 aaagcgtgcc agaaggttaa ccaaacatta aagaataaaa aaagattggc tataacttgc
     8341 gggtatatgt tgagggatta aaaaggcgga agaaatccgc ctcatattgc tgacaaagtg
     8401 cgctttgtcc atgccggatg cggcgcgaac gccttatccg gcctacaaaa gtttgcaaat
     8461 tcaataaatt gcagaattca tgtaggcctg ataagcgaag cgcatcaggc atttttgctt
     8521 ctgtcatcgg tttcaggcta aaggaatctg cctttttccg aaatcattaa tacagttttt
     8581 tcgcgcagtc cagccagtca cctttgaacg gacgcttcat gttttcgata gcgtcgatga
     8641 tgtcgtggtg aaccagctgt tcgttctgga tacctacaca acgaccgccg taacctgcca
     8701 gcagcagatc gatagcgtaa gcgcccatac gggaagccag aatacggtcg taaggcaccg
     8761 gagaaccacc gcgctggatg tggcccagca cagttgcgcg ggtttcacga ccggtttctt
     8821 tctcgatgaa atgcgccagt tcgtcaacat cacacatatg ttcggtaatc gccacgatcg
     8881 cgtgtttttt acctttcgcg atacccgctt tgatttcgtt taccaggtct tcacggctga
     8941 attcaacttc cggaactaca acgaattcac agccaccggc aatggccgca gccagggtca
     9001 gatcgccaca ataacggccc atcacttcca ccacggaaat acgctggtga gaagaagagg
     9061 tgtcacgcag acggtcgatc gcttctacaa cggtgctcag cgcagtgaag aaaccgatag
     9121 tgtagtcagt gcctttgatg tcgttgtcga tagtgcccgg cagaccgatg cacgggaagc
     9181 ccatttcggt cagacgcatt gcacccatgt aggaaccgtc accgccgata accaccagcg
     9241 cgtcgatacc acgttttttc aggttttcga tagccacggc gcggatgttc tcgtcgcgga
     9301 attccgggaa acgcgcagaa ccgaggaacg taccgccacg gttgatcatg tcagacacgc
     9361 tgtaacggtc tagctgtacc atacggtctt catacagacc cagatagccg tcataaatac
     9421 ccattacttc cagaccttct gtcagcgcag aacgaacaac cccgcgaatt gcggcgttca
     9481 tgcctggcgc atcaccgccg cttgtcaaca cacctatttt cttaatcatg actacctctg
     9541 aactttggaa tgcaaaatga aatctgttgc cggaagtctt cttgcacatc gaagtgatcc
     9601 aacgaatgtg caaatagtat aacaatcact tcctgctgaa ttgattcagg tcaggccaaa
     9661 tggcggtatt ttatacacaa aatgcgggtc tggctctctt ttatactgat tatgaaagca
     9721 tagaccgttt accctccctg ggtacgacgg aacaggggtc ctgatggata atcacatccg
     9781 atcccggaaa acgccgtaaa atagcctgct ctacctgatc cgccaccata tgtgcctgaa
     9841 ccaaaggcag agagtcttcc atttccaaat ggatctgaat aaagcgggtc ggccctgact
     9901 gccgcgtgcg aagatcgtga gcgccgctaa cacccggcca ggaagtcacg atatcaataa
     9961 tttcttgccg ttcttcatca ggcaatgcgc gatccagtaa tgactgtacc gcctcatatc
    10021 ccatgcgtaa cgcgctatat aaaatataga tgccgattcc caatgcaaac agagcatcgg
    10081 cgcgatgcca gccgtaccaa gacaacccca gcgccagcag aattgcgccg ttcatcataa
    10141 catcagactg gtaatgtagc atatcagccc gcaccgcctg gctttgcgtc cggcgcacca
    10201 cccaccgctg aaacgagaca aggataatcg tacaaattag cgccacaatt gtcacgataa
    10261 ccccgacgcc tggatctgtc atcggtgttg gagataccag atgttgaata cccgtcaaaa
    10321 acaggaatag tgccgaaccg gagataaaca tactttgcgc cagcgccgcg agggactcag
    10381 ctttaccgtg accaaacgag tgattatcgt cggcaggttg cagggaatat cgcaccacca
    10441 gtaaattcgt caacgacgcg ccgatatcca ccagcgaatc caccagcgcg gcgagaatac
    10501 tcaccgaccc ggtataccac catgcaaaaa ttttaatcag caatagcaac gaagccatcg
    10561 ccgtcgcagc aatcgccgcc cgactgacca gccgtccata agattgattc ataaatactc
    10621 ccgctatcaa ctgacgctag tataacggaa gcaaatcatc tgcaatgcat taagcagcag
    10681 gcaaattgag gataaaaaaa accccacatc atgtggggga agacagggat ggtgtctatg
    10741 gcaaggaaaa cagggtttac tactgggaac gtgagttgct actactcaat agcttcaacg
    10801 atgaactttt ttgccattgc gtcacgtcac gcaactgctc cattcgttgt tgatgtttct
    10861 cgtttaaaac cgcttgctgc tccggcgtta acaggcgata catttggttg cggacttttg
    10921 ccatctcaac ctgacgagca atttgctcat tcgccatttt ttctgcctgt gcgcgcacag
    10981 cgttttcatc aaaattttct gcggtgacaa ggcgatgcat tgtctccagt tcgctaacat
    11041 taacaggagg ctgttcgtgc cgggcctgtt gcataagatc tcgcatctgc tgacgctgat
    11101 gttcggttaa acttatgccg tcgaacatat ggctctgcgt actgcgctgc gtaagttctt
    11161 cacccggatg ccagttatcg cctgaaccga cttcagcagc gtggcttaat gaactgactg
    11221 ccagcgttga ggccatgacg gcagcggtaa ctatgcgcat catttgctcc caaaatcttt
    11281 ctgtcgcgat tcaacgatag agagtttacg attcaggctg caaacatgcg tcagggggtg
    11341 taaaacaacg taaagtcatg gattagcgac gtctgatgac gtaatttctg cctcggaggt
    11401 atttaaacaa tgaataaaat cctgttagtt gatgatgacc gagagctgac ttccctatta
    11461 aaggagctgc tcgagatgga aggcttcaac gtgattgttg cccacgatgg ggaacaggcg
    11521 cttgatcttc tggacgacag cattgattta cttttgcttg acgtaatgat gccgaagaaa
    11581 aatggtatcg acacattaaa agcacttcgc cagacacacc agacgcctgt cattatgttg
    11641 acggcgcgcg gcagtgaact tgatcgcgtt ctcggccttg agctgggcgc agatgactat
    11701 ctcccgaaac cgtttaatga tcgtgagctg gtggcacgta ttcgcgcgat cctgcgccgt
    11761 tcgcactgga gcgagcaaca gcaaaacaac gacaacggtt caccgacact ggaagttgat
    11821 gccttagtgc tgaatccagg ccgtcaggaa gccagcttcg acgggcaaac gctggagtta
    11881 accggtactg agtttaccct gctctatttg ctggcacagc atctgggtca ggtggtttcc
    11941 cgtgaacatt taagccagga agtgttgggc aaacgcctga cgcctttcga ccgcgctatt
    12001 gatatgcaca tttccaacct gcgtcgtaaa ctgccggatc gtaaagatgg tcacccgtgg
    12061 tttaaaacct tgcgtggtcg cggctatctg atggtttctg cttcatgata ggcagcttaa
    12121 ccgcgcgcat cttcgccatc ttctggctga cgctggcgct ggtgttgatg ttggttttga
    12181 tgttacccaa gctcgattca cgccagatga ccaagcttct ggatagcgaa cagcgtcagg
    12241 ggctgatgat tgagcagcat gtcgaagcgg agctggcgaa cgatccgccc aacgatttaa
    12301 tgtggtggcg gcgtctgttc cgggcgattg ataagtgggc accgccagga cagcgtttgt
    12361 tattggtgac caccgaaggc cgcgtgatcg gcgctgaacg cagcgaaatg cagatcattc
    12421 gtaactttat tggtcaggcc gataacgccg atcatccgca gaagaaaaag tatggccgcg
    12481 tggaactggt cggtccgttc tccgtgcgtg atggcgaaga taattaccaa ctttatctga
    12541 ttcgtccggc cagcagttct caatccgatt tcattaactt actgtttgac cgcccgctat
    12601 tactgctgat tgtcaccatg ttggtcagta cgccgctgct gttgtggttg gcctggagtc
    12661 tggcaaaacc ggcgcgtaag ctgaaaaacg ctgccgatga agttgcccag ggaaacttac
    12721 gccagcaccc ggaactggaa gcggggccac aggaattcct tgccgcaggt gccagtttta
    12781 accagatggt caccgcgctg gagcgcatga tgacctctca gcagcgtctg ctttctgata
    12841 tctctcacga gctgcgcacc ccgctgacgc gtctgcaact gggtacggcg ttactgcgcc
    12901 gtcgtagcgg tgaaagcaag gaactcgagc gtattgaaac cgaagcgcaa cgtctggaca
    12961 gcatgatcaa cgatctgttg gtgatgtcac gtaatcagca aaaaaacgcg ctggttagcg
    13021 aaaccatcaa agccaaccag ttgtggagtg aagtgctgga taacgcggcg ttcgaagccg
    13081 agcaaatggg caagtcgttg acagttaact tcccgcctgg gccgtggccg ctgtacggca
    13141 atccgaacgc cctggaaagt gcgctggaaa acattgttcg taatgctctg cgttattccc
    13201 atacgaagat tgaagtgggc tttgcggtag ataaagacgg tatcaccatt acggtggacg
    13261 acgatggtcc tggcgttagc ccggaagatc gcgaacagat tttccgtccg ttctatcgga
    13321 ccgatgaagc acgcgatcgt gaatctggcg gtacaggatt ggggctggca attgttgaaa
    13381 ccgccattca gcagcatcgt ggctgggtga aggcagaaga cagcccgctg ggcggtttac
    13441 ggctggtgat ttggttgccg ctgtataagc ggagttaaac tccgcatttg taggcaggat
    13501 aaggcgttta cgccgcatcc ggcatttgag caggatgcct gatgcgacgc tgatagcgtc
    13561 ttatcaggcc tacactccgg gggttatttt ccccacaacc ggcgggaaaa atcttcgatc
    13621 ttgccgctca gtcggcgctt ttgcatcgtc cgcgtccagc ttttcgataa cccggcggcg
    13681 gagagtaaac ggtgatactg atcgtcatca aacggcatat gccatgcgat ggcggcagcc
    13741 tcctgcacgg tcacatcgct gacacgggaa accaactcca gcggcgcgtc cgcagacact
    13801 ttccctggcg ctatcacgct atacaaccac cccaccttac ctgtgttttg catcaactgc
    13861 gctatatcgc tgatatcaaa atggtaattg agtttatagc aaggcgagcg cggctggctg
    13921 acctgaatta atgcctctcc ccagcggaaa atatcgccca tatgaacatt actttccgtg
    13981 agaccgtcgg ttgagagatt ttcgccaaac gcaggcgcaa caaacaactc cgcctgttcc
    14041 ggaaattccc gcacccagta gagatagtgc tcacgaggat aatgacacag cgctctgtct
    14101 ggcccgccgt gaaccttttt ctccgcctgc tcgtcacctt ccagccccag ctccgtcagc
    14161 atcaactcac catcaacctg gattttggca attgcgctgg gtttgccttc gggataagcc
    14221 tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct gttccctctc cacgagaaat
    14281 ttccccatta gaccatattt attttatgtc agccgtgctg ttttcgcggt aagtatccag
    14341 gcaggaactc aatagcaaac cggacgaacg ttagttcatc cggctgtggg gattaaggaa
    14401 gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt tgactttacg
    14461 tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg ccgtcgctac
    14521 cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg caatcagcac
    14581 aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag tgccatcgcc
    14641 accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa ttattgccac
    14701 acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa tggtattgcc
    14761 cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa attctcgtaa
    14821 ttcagggtca agattcccca gggcaaagcc caccaggaac ggtaatactg cgccgacgaa
    14881 aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg tcatgagcgg
    14941 cccggactcc aacgacatca acacaaatgc cccagcttct tctttcgtac cgtactgctg
    15001 catgatggaa gcgtaaagtc cgccgttggt catatccatc gccgccacca gcgccagcgt
    15061 tgaaagtccg gcaaagaatc caacttcaac accatgttcc ggaataatgc gcgaggcaat
    15121 tgccgcaacc acccacgcga cggcaatttt agttaccacc agcgtaccgg atttacgcag
    15181 taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc acaccgccag
    15241 aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt tccccgcccc
    15301 cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca tcccccccgg
    15361 gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata tttttaaggg
    15421 ctgatgcgta attcctcaat ggagcatcag tgttgatgga aacgaagata gacattcatt
    15481 taatcaataa aagtgatcaa aaccacatca attgaaacgc tgttttattt ttataggatc
    15541 attgttttta ttttggaatt gttcacaaaa aaaccaccgc cgttggcgat ggttcatttt
    15601 ttaagctgat atgcggccta tacgcctcat tgcagcaggc ggcaaatgat tattttttcg
    15661 ccgcaaaacg tgccgctgct tcgtcccagt tcaccacgtt ccagaactct ttgatgtagt
    15721 ccggacggcg gttctggaat ttcaggtagt aagcatgttc ccacacatcc aggcccagaa
    15781 tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggagaatcc tggttagcag
    15841 tagaaaccac cgccagttta tcgcctttca gcaccagcca tgcccagccg gaaccaaagc
    15901 gggaagctgc cgctttttca aattctgctt tgaagttatc aacggagccg aagtcacgtt
    15961 cgatagccgc tttcaggtca ccctgcaggg tggtgccttt tttcagacct ttccagaaca
    16021 ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg tctgctggca
    16081 gctggtccag tttggtgatc agctcttcaa ccggcaggtt ggcaaattct ggcaggcttt
    16141 ccagcgccgc gttggcgttg tttacgtagg tctgatggtg tttggtgtgg tggatttcca
    16201 tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg gatggcaggg
    16261 tatagctcat attcatctcc agtattgtcg ggcggccgat tgttaatgcc gcgtaagcag
    16321 ttggtttatt atagttaatt aaatgatatt gaaaatgatt atcaatgccg cacttttcgt
    16381 aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca cttaatgccg
    16441 tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac agcacgattt
    16501 ttacactcat ctcgtcggag atgtgaagcg acgaaaaatg atgaggataa gaagatgagt
    16561 aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc agcctgtttt
    16621 tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc agtcggtggg
    16681 attgtttcgt ggattattct gccgtgggcc atcagcgccc tgttactacc ggatttctgg
    16741 gcgtattaca gctcgtttag tctctctacg ctgctgcctg tttttctgtt cggcgctatg
    16801 tgggggatcg gtaatatcaa ctatggcctg accatgcgtt atctcggcat gtcgatggga
    16861 attggcatcg ccattggcat tacgttgatt gtcggtacgc tgatgacgcc aattatcaac
    16921 ggcaatttcg atgtgctgat taacaccgaa ggcggacgca tgacgttact cggcgttctg
    16981 gtggcgctga ttggcgtagg gattgtaact cgagccgggc agttgaaaga gcgcaagatg
    17041 ggcattaaag ccgaagagtt caatctgaaa aaagggctgg tgctggcggt gatgtgcggc
    17101 attttctctg ccgggatgtc ctttgcgatg aacgccgcaa aaccgatgca tgaagccgct
    17161 gccgcacttg gcgtcgatcc actgtatgtc gctctgccaa gctatgttgt catcatgggc
    17221 ggcggcgcga tcattaacct cggtttctgt tttattcgtc tggcaaaagt gaaggatttg
    17281 tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt gttactctcg
    17341 gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctaggg ccacgcccgc
    17401 attccggcgc agtatgacta catcagttgg atgctgcata tgagtttcta tgtattgtgc
    17461 ggcggtatcg tcgggctggt gctgaaagag tggaacaata caggacgccg tccggtaacg
    17521 gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcta acatcgtcgg catcggcatg
    17581 gcgaattaat ctttctgcga attgagatga cgccactggc tgggcgtcat cccggtttcc
    17641 cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt cgaaatatca
    17701 ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct gacctgccgc
    17761 agatattgat tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa aacgcgctca
    17821 ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag gctagccgcc
    17881 agccgggtaa tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa cgaatcactg
    17941 gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc agccatttcg
    18001 ttagcaaacg gcacatgctg actactttca tgctcaagct gaccgataac ctgccgcgcc
    18061 tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc gctaaatccc
    18121 ggaatcgctc cctgccagtc aagattcagc ttcagccgct ccgggcaata aataatattc
    18181 tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg aatataaaag
    18241 agatcgccac gggtaatgcg ataaggacga tcgttgagta catgcagacc attaccgcgc
    18301 cagacaatca ccagctcaca aaaatcatgt gtatgttcgg caaagacatc ttgcggataa
    18361 cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt
    18421 aactgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt cgcaatatgg
    18481 cgtacaaata cgttgagaag attcgcgtta ttgcagaaag ccatcccgtc cctggcgaat
    18541 atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct
    18601 gaatccacag cgataggcga tgtcagtgac gctggcctcg ctgtggcgta gcagatgtcg
    18661 ggctttcatc agtcgcaggc ggttcaggta tcgctgaggc gtcagtcccg tttgctgctt
    18721 aagctgccga tgtagcgtac gcagtgaaag agaaaattga tccgccacgg catcccaatt
    18781 cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac gtgatgcgct
    18841 gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt gcataaacaa
    18901 gatctcgcga ctggcggtcg agggtaaatc attttcccct tcctgctgtt ccatctgtgc
    18961 aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtgag acggatactg
    19021 cccatccagc tcttgtggca gcaactgatt cagcccggcg agaaactgaa atcgatccgg
    19081 cgagcgatac agcacattgg tcagacacag attatcggta tgttcataca gatgccgatc
    19141 atgatcgcgt acgaaacaga ccgtgccacc ggtgatggta tagggctgcc cattaaacac
    19201 atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat gatgttcagg
    19261 aaaatccgcc tgcgggagcc ggggttctat cgccacggac gcgttacaag acggaaaaaa
    19321 atccacacta cgtaatacgg tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt
    19381 aatcacgagg tcaggttctt accttaaatt ttcgacggaa aaccacgtaa aaaacgtcga
    19441 tttttcaaga tacagcgtga attttcagga aatgcggtga gcatcacatc accacaattc
    19501 agcaaattgt gagcatcatc acgttcatct ttccctggtt gccaatggcc cattttcctg
    19561 tcagtaacga gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat gaaattcagc
    19621 aggatcacat tatgaccttt cgcaattgtg tcgccgtaga tctcggcgca tccagtgggc
    19681 gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc gaaatccatc
    19741 gttttaacaa tgggctgcat agccaaaacg gttatgtcac ctgggatgtg gatagcctgg
    19801 aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt atcgatagca
    19861 ttgggattga tacctggggc gtggactttg tgctgctcga ccaacagggt cagcgtgtgg
    19921 gcctgcccgt tgcttatcgc gatagccgca ccaatggcct gatgccgcag gcacaacagc
    19981 aactcggcaa acgcgatatt tatcagcgta gcggcatcca gtttctaccc ttcaatacgc
    20041 tttatcagtt gcgtgcgctg acggagcaac aacctgaact tattccacac attgctcacg
    20101 ctctgctgat gccggattac ttcagttatc gcctgaccgg caagatgaac tgggaatata
    20161 ccaatgccac caccacacaa ctggtcaata tcaatagcga cgactgggac gagtcgctac
    20221 tggcgtggag cggggccaac aaagcctggt ttggtcgccc gacgcatccg ggtaatgtca
    20281 ttgggcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt gccagccatg
    20341 ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgccgct tatctctctt
    20401 ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat gacacggcgc
    20461 tggcagccaa catcaccaat gaaggcgggg cggaaggtcg ctatcgggtg ctgaaaaata
    20521 ttatgggctt atggctgctt cagcgagtgc ttcaggaacg gcaaatcaac gatctcccgg
    20581 cgcttatcgc cgcgacacag gcacttccgg cctgccgctt catcatcaat cccaatgacg
    20641 atcgctttat caatcctgac gagatgtgca gcgaaattca ggctgcgtgt cgggaaacgg
    20701 cgcaaccgat cccgggaagt gatgctgaac tggcgcgctg tattttcgac agtctggcgc
    20761 tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg cggtgaagat ttctcgcaac
    20821 tgcatattgt cggcggcggc tgccagaaca cgctgctcaa ccagctatgc gccgatgcct
    20881 gcggtattcg ggtgatcgcc gggcctgttg aagcctcaac gctcggcaat atcggcatcg
    20941 agttaattac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc gtcagcacca
    21001 ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcccac tatgtggcgc
    21061 agattcactc tacacgacag acaaaggagc tttgcgcatg accactcaac tggaacaggc
    21121 ctgggagctg gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg aggcgctgcg
    21181 ccaacttgat cgtttacccg tttcaatgca ctgctggcag ggcgatgatg tttccggttt
    21241 tgaaaacccg gaaggttcgc tgaccggggg gattcaggcc acaggcaatt atccgggcaa
    21301 agcgcgtaat gccagtgagc tacgtgccga tctggaacag gctatgcggc tgattccggg
    21361 gccgaaacgg cttaatttac atgccatcta tctggaatca gatacgccag tctcgcgcga
    21421 ccagatcaaa ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca atcagctcgg
    21481 tctggatttt aacccctcct gcttttcgca tccgctaagc gccgatggct ttacgctctc
    21541 gcatcccgac gacagcattc gccagttctg gattgatcac tgcaaagcca gccgtcgcgt
    21601 ttcggcctat tttggcgagc aactcggcac accatcggtg atgaacatct ggatcccgga
    21661 tggtatgaaa gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc tggcagcact
    21721 ggatgaggtg atcagcgaga agctaaaccc tgcgcaccat atcgacgccg ttgagagcaa
    21781 attgtttggc attggcgcag agagctacac ggttggctcc aatgagtttt acatggggta
    21841 tgccaccagc cgccagactg cgctgtgcct ggacgccggg cacttccacc cgactgaagt
    21901 gatttccgac aagatttccg ccgccatgct gtatgtgccg cagttgctgc tgcacgtcag
    21961 ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg gatgatgaaa cccaggcaat
    22021 tgccagtgag attgtgcgtc acgatctgtt tgaccgggtg catatcggcc ttgacttctt
    22081 cgatgcctct atcaaccgca ttgccgcgtg ggtcattggt acacgcaata tgaaaaaagc
    22141 cctgctgcgt gcgttgctgg aacctaccgc tgagctgcgc aagctggaag cggcgggcga
    22201 ttacactgcg cgtctggcac tgctggaaga gcagaaatcg ttgccgtggc aggcggtctg
    22261 ggaaatgtat tgccaacgtc acgatacgcc agcaggtagc gaatggctgg agagcgtgcg
    22321 ggcttatgag aaagcgattt tgagccagcg tgggtaaaca ctgccggatg cggcacaagc
    22381 gccttatccg gcctacgggt cgaatgtcca tgcgggctgt caccctcacc ccggccctct
    22441 ccctgaaagg gcgagggaga aaaacatagc ctcgtgcggc aacggagctg aacaccgctc
    22501 cggacagtcc cctcgcccct acggggagag ggttagggtg aggggaacaa gccgcaccat
    22561 gtctaaacaa ggcaacctaa tttacaggaa cacagaacat gcaaaacatt acccagtcct
    22621 ggtttgtcca gggaatgatc aaagccacca ccgatgcctg gctgaaaggc tgggacgagc
    22681 gcaacggcgg caacctgacg ctgcgcctgg atgacgccga tatcgcgctt tatcacgaca
    22741 acttccaccc acaaccgcgc tatattccgc tcagccaacc catgccttta ctggcaaata
    22801 cgccgtttat tgtcaccggc tccggcaaat tcttccgtaa cgtccagctc gatcctgcag
    22861 ctaacttagg cgtcgtaaaa gtcgacagcg atggcgcggg ctaccatatt ctctgggggt
    22921 taaccaacga agccgttcca acttctgaac ttccggctca ctttctctcc cactgcgagc
    22981 gcattaaagc caccaacggt aaagatcggg tgatcatgca ctgccacgcc accaacctga
    23041 tcgccctcac ctatgtactg gaaaacgaca ccgcggtctt tactcgccaa ctgtgggaag
    23101 gcagcaccga atgtctggca gtattcccgg atggcgttgg cattctgccg tggatggtgc
    23161 ccggcacgga cgaaatcggc caggcgaccg cacaagagat gcaaaaacat tcgctggtgt
    23221 tgtggccctt ccacggtgtg ttcggttccg gaccgacgct ggatgaagcc tttggtttaa
    23281 tcgacaccgc agaaaaatca gcacaagtat tagtgaaggt ttattcgatg ggcggcatga
    23341 aacagaccat cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc gttacaccac
    23401 tagccagtgc gctggcgctg taaggagcaa acatgatccg caaagccttt gtcatgcagg
    23461 taaaccccga cgcccacgaa gagtatcagc gtcggcataa tcccatctgg ccggaactgg
    23521 aagcagtgtt gaaatctcac ggtgtgcata actacgccat ttacctcgac aaagcgcgta
    23581 atctgctgtt tgccatggta gagattgaat ctgaagaacg gtggaatgcg gtagccagta
    23641 ctgatgtttg ccaacgttgg tggaaatata tgaccgatgt tatgcccgct aacccagata
    23701 acagcccggt gagtagcgag ctgcaagaag tgttttacct gccgtaattg cccaatgtgc
    23761 cggagatgcc tccggcacaa tattttaaat ttcgatttga aaaattaaac atattacatc
    23821 tcccggtaag ttatatttcc ctgatacatt gtgagtaaat cacaaaaata atgaataacc
    23881 cattaatgat tcatgtggtt tatttaaata accattatgt gcattactcc gcaaatctga
    23941 cctttcactc tgtctcaaat tgtcattgtt taccgacata tcgcctctca tcacttaatc
    24001 gctccaccat tacaattgat taaaatatat tttatggagt ccgattaatg gcagctctta
    24061 ctgcaagctg tattgacctg aatattcagg gcaatggcgc ttattccgtt ctgaagca
//
LOCUS       DAJTNM010000053        23925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTNM010000053 DAJTNM010000000
VERSION     DAJTNM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.053"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..336
                     /locus_tag="OO172_002200"
     CDS             <1..336
                     /locus_tag="OO172_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5512379.1"
                     /translation="KGNGIPANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGKELPQN
                     LPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKA
                     LNELEHQTK"
     gene            389..2119
                     /locus_tag="OO172_002201"
     CDS             389..2119
                     /locus_tag="OO172_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512380.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWMKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(2179..2670)
                     /gene="yajQ"
                     /locus_tag="OO172_002202"
     CDS             complement(2179..2670)
                     /gene="yajQ"
                     /locus_tag="OO172_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5512381.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2838..3749
                     /gene="panE"
                     /locus_tag="OO172_002203"
     CDS             2838..3749
                     /gene="panE"
                     /locus_tag="OO172_002203"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5512382.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3712..4302
                     /gene="yajL"
                     /locus_tag="OO172_002204"
     CDS             3712..4302
                     /gene="yajL"
                     /locus_tag="OO172_002204"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5512383.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4356..5804)
                     /gene="thiI"
                     /locus_tag="OO172_002205"
     CDS             complement(4356..5804)
                     /gene="thiI"
                     /locus_tag="OO172_002205"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5512384.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            6010..6252
                     /gene="xseB"
                     /locus_tag="OO172_002206"
     CDS             6010..6252
                     /gene="xseB"
                     /locus_tag="OO172_002206"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5512385.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            6252..7151
                     /gene="ispA"
                     /locus_tag="OO172_002207"
     CDS             6252..7151
                     /gene="ispA"
                     /locus_tag="OO172_002207"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5512386.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            7176..9038
                     /gene="dxs"
                     /locus_tag="OO172_002208"
     CDS             7176..9038
                     /gene="dxs"
                     /locus_tag="OO172_002208"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5512387.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            9093..10067
                     /gene="yajO"
                     /locus_tag="OO172_002209"
     CDS             9093..10067
                     /gene="yajO"
                     /locus_tag="OO172_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5512388.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEENSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(10121..10639)
                     /gene="pgpA"
                     /locus_tag="OO172_002210"
     CDS             complement(10121..10639)
                     /gene="pgpA"
                     /locus_tag="OO172_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5512389.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10617..11594)
                     /gene="thiL"
                     /locus_tag="OO172_002211"
     CDS             complement(10617..11594)
                     /gene="thiL"
                     /locus_tag="OO172_002211"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5512390.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDVLSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11672..12091)
                     /gene="nusB"
                     /locus_tag="OO172_002212"
     CDS             complement(11672..12091)
                     /gene="nusB"
                     /locus_tag="OO172_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5512391.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(12111..12581)
                     /gene="ribE"
                     /locus_tag="OO172_002213"
     CDS             complement(12111..12581)
                     /gene="ribE"
                     /locus_tag="OO172_002213"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5512392.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGSAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12670..13773)
                     /gene="ribD"
                     /locus_tag="OO172_002214"
     CDS             complement(12670..13773)
                     /gene="ribD"
                     /locus_tag="OO172_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5512393.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13777..14226)
                     /gene="nrdR"
                     /locus_tag="OO172_002215"
     CDS             complement(13777..14226)
                     /gene="nrdR"
                     /locus_tag="OO172_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5512394.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14377..14916
                     /locus_tag="OO172_002216"
     CDS             14377..14916
                     /locus_tag="OO172_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5512395.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15215..16099
                     /gene="tsx"
                     /locus_tag="OO172_002217"
     CDS             15215..16099
                     /gene="tsx"
                     /locus_tag="OO172_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5512396.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16276..16623)
                     /locus_tag="OO172_002218"
     CDS             complement(16276..16623)
                     /locus_tag="OO172_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5512397.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16752..17723)
                     /gene="secF"
                     /locus_tag="OO172_002219"
     CDS             complement(16752..17723)
                     /gene="secF"
                     /locus_tag="OO172_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5512398.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17734..19581)
                     /gene="secD"
                     /locus_tag="OO172_002220"
     CDS             complement(17734..19581)
                     /gene="secD"
                     /locus_tag="OO172_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5512399.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19609..19941)
                     /gene="yajC"
                     /locus_tag="OO172_002221"
     CDS             complement(19609..19941)
                     /gene="yajC"
                     /locus_tag="OO172_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5512400.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19964..21091)
                     /gene="tgt"
                     /locus_tag="OO172_002222"
     CDS             complement(19964..21091)
                     /gene="tgt"
                     /locus_tag="OO172_002222"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5512401.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDMHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(21146..22216)
                     /gene="queA"
                     /locus_tag="OO172_002223"
     CDS             complement(21146..22216)
                     /gene="queA"
                     /locus_tag="OO172_002223"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5512402.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22309..22882
                     /gene="acpH"
                     /locus_tag="OO172_002224"
                     /pseudo
     CDS             22309..22882
                     /gene="acpH"
                     /locus_tag="OO172_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(22887..>23925)
                     /gene="malZ"
                     /locus_tag="OO172_002225"
     CDS             complement(22887..>23925)
                     /gene="malZ"
                     /locus_tag="OO172_002225"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5512403.1"
                     /translation="DRHNLGTGGACHNSESPWRDWYLFSDDGTALDWLGYASLPKLDY
                     QSESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARNNMQHVAGITEAA
                     KETQSEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGFLANTDISYDPQQIDAQ
                     TCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIARLPLAVVWLFTWPGVP
                     CIYYGDEVGLDGKNDPSCRKPFPWQVEKQDTALFALYRRMIVLRKKSQALRRGGCQVL
                     YAEDNVVVFVRVLNQQRVLVAINRGEASEVVLPASPFLNAVQWQCKEGHGQLTDGILA
                     LPAISATVWMN"
BASE COUNT         6082 a         6270 c         5879 g         5694 t
ORIGIN      
        1 aaaggcaatg gtatacctgc aaacaatgaa aaatacctag catatctgca acaagctgca
       61 acactgggca gtagtaatgc ccaggcggaa attggctatt tatatctcat cggaaaagaa
      121 ctaccacaga atcttcctga tgctggtgtc tggttcaaaa aagcagctgc acaaggtaat
      181 tttgccgcac atttctatct gggaaggatg tatcagaatg gtgacggcgt tgagagaaat
      241 atggaaaaag ccaggtttca tctatctaat gcggcagaag gtggaataga acctgcgctt
      301 aaagcattaa atgaacttga acatcaaaca aaataaaacg ctcagggacc acgtaaggtt
      361 catcaaacag aaaaggatat agtttgcgat gaaagcgaaa tctactttgt tattaatgat
      421 tttatctgtt ttttattctc atcaggtttt atccagcgac cccactcagc cggatgttta
      481 ttctgtcgtc gagaaaaaac taaactctgc tttacctctg gaagaaaata ccaccttcag
      541 atcgcaggct gaatggttta atttgcaata tgaattatta gtatctggct accctgaacg
      601 tgcttacaat ttactgagca aacttgagac agaatcgaaa aacgctaaat attatctgga
      661 tttaactgcc tccagagtcg ctgaagaaga agcaccagag acagccttaa cttttttatc
      721 aaaaattggg ttaaataaac cggctactct tacatccttt gagagctata tcaatagctg
      781 gatgaaaagc aaacaacccg aaaaggctat gcagcttctc agcctcgatg agaatgccct
      841 gaattattat ttaccgactg tgttagtcgc atatcagatc gttcctgata aggcaataag
      901 tatttataat tctgtatatg gagataatgt tgttataccc tatcagcaag tcaccatgtt
      961 acttacagtt gcggagggtt atcaaacaaa aggcgatata aaaaacgcca ttcattatgc
     1021 cgataaggcg ctgaatatgt ttgataaagc tattgctgta gatagcagtc gtgaatccca
     1081 ttatagtgat gagtatttga aactcatgaa tatctacgcc gccaatggca acaaagaaaa
     1141 agcggtggtt ttatctcaac gtctacacaa agccattacc gaatctcaat cgaattactt
     1201 aagtactctg gaaggaattt tattgtttta tagaaataat gatatgcagc aagactatca
     1261 aaacagcctt tcaaattaca tagtatttat cgatgaaatt tttgcctttt ccccatcaac
     1321 aagaagtgaa ttaagcctca ttaatttatt aaattcatta aatgaggtgg aattgatgag
     1381 aaaacgtctg gcgtttctga tgacagcacc agaatacgct tgttatgata gtcagtattg
     1441 ttatgaagac aaagtcagcg cattaaaaat cctgtatcgt catcatgaca atgagctaat
     1501 ggacaaatat ttcacgactc ttcttaaaga gatcagtaat caaccattag aagactggga
     1561 tataataata agtaatactg gtgaaaaatt tagtgaagtg ggtctaacca aatatgctca
     1621 acatctcgca tctttcgctg aagaatttta tataaaagag caaaaatcac attctgaaag
     1681 tgaaatgaga ggactatttt catcacttgc cgaattatat agttttggta atgatactgc
     1741 cagcgcaaac cgagtgttcc atcagcatgt ccctgccttc aatacagatc atatgattca
     1801 atattttata aatgccaaag aatggaacaa tgccagggaa ttattgataa aagaggaaat
     1861 gttgggtatg cataatctga tattgttgga gaatatttgt atgcaaaaaa atgcagaatg
     1921 catggcacac attaccttta cgcttaataa attaacaaca caaccagcaa taacaaaaat
     1981 agatagcgtg ggtaatgagc aactttatca gattggcaaa atctaccata agttggaaat
     2041 taaacctgag ccagagcaac aggttttaat tcagtcgctt tatgaccggg catcaggaag
     2101 cgcctctgca actcaataaa tgcatggtca aaagggccac cacggtggcc ctgacaatca
     2161 gtagctatat ccagaaagtt aatcgcggaa gtttttgaac tggaacggct gaccgagatc
     2221 gccaccgcgc accatcgcca tgacagcctg caaatcatca cgagatttgc cagtcacgcg
     2281 gatctcatcg ccctgaattt gcgcctgcac tttcagtttg ctgtctttga tcatcttgac
     2341 gatttttttc tgagtggcgc tctcaatgcc ctgtttcagt ttcgcttcca caaaccaggt
     2401 tttaccgcta tgaacgatat tttccggtac atccagtgaa ctgccttcaa tgccgcgctt
     2461 cagcagcttg gcacgcagaa tatccagcaa ctgattgacc tggaagtcgg actcgctcaa
     2521 cactttgatg gttttgctgg cgtcgttcag ctcaaatgag gcttcaacgt tacggaagtc
     2581 aaaacgggac tccacttcgc gactcgcgtt atcgaccgcg ttacgtgctt cctgaagatc
     2641 aacttcagag acaatatcga aagatggcat cttttcctct cccttcattt ttgatgcgaa
     2701 gcataatacc tgcaaagtta aataaccaac agtcctgcac caggacgtat cccttaattg
     2761 tgcatagtcg caacagcatg cacaattaag ggatagcctt attgaacacc cggagtggtt
     2821 gtgggtgagg aggaacaatg aaaattaccg tattgggatg cggtgcctta gggcaattat
     2881 ggcttacagc actttgtaaa cagggtcatg aagttcaggg ctggctgcgc gtaccgcaac
     2941 cttattgtag tgtgaatctg gttgagacag atggttcgat atttaacgaa tcgctgaccg
     3001 ccaacgatcc cgattttctc gccaccagcg atctgctcct ggtgacgctg aaagcatggc
     3061 aggtttccga tgccgtcaaa agcctcgcat ccacactgcc tgtaactacg ccaatactgt
     3121 taattcacaa cggcatgggc accatcgaag aattgcaaaa cattcagcag ccattactga
     3181 tgggcaccac cacccatgcc gcccgccgcg acggcaatgt cattattcat gtggcaaacg
     3241 gaatcacgca tatcggtccg gcacggcaac aggatggcga ttacagttat ctggcggata
     3301 ttttgcaaac cgtgttgcct gacgttgcct ggcataacaa tattcgcgcc gagctgtggc
     3361 gcaagctggc cgtcaactgc gtgattaatc cactgactgc catctggaat tgcccgaacg
     3421 gtgaattacg tcatcatccg caagaaatta tgcaaatatg cgaagaagtc gcggcagtga
     3481 tcgaacgcga agggcatcat acttcagcgg aagatttgcg tgattacgtg atgcaggtga
     3541 ttgatgccac agcggaaaat atctcgtcga tgttgcagga tatccgcgcg ctgcgccaca
     3601 ccgaaatcga ctatatcaat ggttttctct tacgccgcgc ccgcgcgcat gggattgccg
     3661 taccggaaaa cacccgcctg tttgaaatgg taaaaagaaa ggagagtgaa tatgagcgca
     3721 tcggcactgg tttgcctcgc ccctggtagt gaagagactg aagccgtcac cacgatcgac
     3781 ctcttggttc gcggcggtat caaagtcacc actgccagcg tcgacagcga tggcaacctg
     3841 gcaattacct gctcgcgcgg cgtgaagctg ctggcggatg cgccgctggt cgaagtggct
     3901 gatggcgaat atgacgtgat cgtgctgcct ggtggcatta aaggcacgga gtgttttcgc
     3961 gatagcactc tgctggttga aaccgttaaa cagttccacc gttccgggcg tatcgtcgcg
     4021 gctatttgcg ccgcgccagc caccgtgctg gtgccgcacg atatctttcc gattggcaat
     4081 atgaccggct tcccgacgct gaaagacaaa attcccgctg aacaatggca ggacaagcgc
     4141 gtcgtctggg atgcacgggt aaaattgctg accagccagg ggccgggtac agctatcgac
     4201 tttggtctga aaattatcga cctgctggtt gggcgcgaga aagcccatga agtggcatca
     4261 caactggtga tggcggcagg gatttataat tattacgagt agtgtcggat gcggcaaacg
     4321 tcgcatctga ccagatgcga cgtaaaaact acaaattacg ggcgatatac cttcacattg
     4381 ttaaagcctt gctcgcgcag atagagcgcc tgcagacggc tcatcacccc gcgctcacac
     4441 cacagcagcc aggttttgtt ctggtcgaga tcgccaaatt tggtgctcag tttatagaac
     4501 ggcagagaaa ccacatcaat cccttcgact ttcagtggct tatcttcctg ttcatcgaca
     4561 gaacggatat cgagaatcac gtcgttcggg ccgaagccgt tgacggtttc cacttccacc
     4621 acttcctgct cggtctgctg ggcgatttcg cggatatcaa cgttattcgc ttcctcaacc
     4681 actttatcga gaatactgaa gtcgaacttc tcttcttccg cttcaatctt cgatttaacc
     4741 gctttcaccg tcgggctttt ggagatcacg ccacaatatt ccggcatcgt gcgagcaaag
     4801 tcttcggtgc caatctggcg agccaggttg atgatgtgct ctttgtcgta agaaatcagc
     4861 ggacgcagga tcagcgtgtc agagacgtta tcaatcaggc gcaggttggt cagcgtctga
     4921 ctggacacct ggccgagcgc ttcgccggtg accagcgcct gtacgccgta acgttcagcc
     4981 actttagatg cggcacgcac catcatacgt ttgaggataa cgcccatctg accgtcgtcg
     5041 attttctcga gaatttcccc gacaaccggt tcgaaattaa tggcgacaaa acgcacgcgg
     5101 tgggagctgc caaagcggtt ccacagataa tgcgccacct gacgcacgcc aatttcatgc
     5161 gctgcgccgc cgaggttaaa gaagcagtaa tgcacgcggc agccgcgacg catcaacata
     5221 taactggaaa caccggagtc gaaaccaccg gaaatgagcg acagcacatc ttcctgggtg
     5281 ccaatcggga aaccaccaat accttcgtag cgacctttaa tcagcaggag gcgatcgtct
     5341 tccacttcca gatgaaccgt tacatccggg ttagtcagct tcacgcgcgc ggattcaata
     5401 tgctgattta aaccgccgcc gacgtaacgt tccacatcaa tcgagctaaa atcatgtttg
     5461 ccacggcgct tcacgcgtac gcagaaggtt ttgccttcca gctgatcgcg atactgaacc
     5521 aacgctttct caaaaatatc gtgcatgtcg gtaaacggca cgtcttcgac ttcgagaata
     5581 tggtggatac ccggaatacg ggtcagggcg tcgcgaatag tcagacgctg gttttcatct
     5641 ttcgcgcgaa cttcgatgtt atcccagtgg cggacgacag cgagcgtctc atcatagtgc
     5701 tttaaaacgt tacgaatgtt cccggtaagg atttttataa agcgcaagcg cacagattgg
     5761 cttttgatgg tgatttccgg gaacaattta atgataaact tcatggcggc aatggttcgt
     5821 tggcaagcct taagcgactt gtatagggaa aaatacagca gcccacacct gcggctgcat
     5881 ccaggcgcgg aagtatacca ctaacatcgc tttgctgcgc acatcacctt accattgcgc
     5941 gttatttgct atttgccctg agtccgttac catgacgggg cgaaaaatat tgagagtcag
     6001 acattcatta tgccgaagaa aaatgaggcg cccgccagct ttgaaaaggc gctgagcgag
     6061 ctggaacaga ttgtaacccg tctggaaagt ggcgacctgc cgctggaaga ggcgctgaac
     6121 gagttcgaac gcggcgtgca gctggcacgt caggggcagg ccaaattaca acaagccgaa
     6181 cagcgcgtac aaattctgct gtctgacaat gaagacgcct ctctaacccc ttttacaccg
     6241 gacaatgagt aatggacttt ccgcagcaat tcgaagcctg cgttaagcag gccaaccagg
     6301 cgctgagccg ttttatcgcc ccactgccct ttcagaacac tcccgtggtc gaaaccatgc
     6361 agtatggcgc attattaggt ggtaagcgcc tgcgaccttt cctggtttat gccaccggtc
     6421 atatgtttgg cgttagcaca aacacgctgg acgcacccgc tgctgccgta gagtgtatcc
     6481 acgcttactc attaattcat gatgatttac cggcgatgga tgatgacgat ctgcgtcgcg
     6541 gtttgccgac ctgccatgtg aagtttggcg aagcaaacgc gattctcgct ggcgacgctt
     6601 tacaaacgct ggcgttctcg attctaagcg atgccaatat gccggaagtg tcggatcgcg
     6661 acagaatttc gatgatttct gaactggcga gcgccagcgg tattgccgga atgtgcggtg
     6721 gtcaggcatt agatttagac gcggaaggca aacacgtacc tctggacgcg cttgagcgta
     6781 ttcatcgtca taaaaccggc gcattgattc gcgccgccgt tcgccttggt gcattaagcg
     6841 ccggagataa agggcgtcgt gctctgccag tactcgacaa gtacgcagag agcatcggcc
     6901 ttgccttcca ggttcaggat gacatcctgg atgtggtagg agatactgca acgttgggaa
     6961 aacgccaggg tgccgaccag caacttggta aaagtaccta ccctgcactt ctgggtcttg
     7021 agcaagcccg gaagaaagcc cgggatctga tcgacgatgc ccgtcagtcg ctgaaacaac
     7081 tggctgaaca gtcactcgat acctcggcac tggaagcgct agcggactac atcatccagc
     7141 gtaataaata aacaataagt attaataggc ccctgatgag ttttgatatt gccaaatacc
     7201 cgaccctggc actggtcgac tccacccagg agttacgact gttgccgaaa gagagtttac
     7261 cgaaactctg cgacgaactg cgccgctatt tactcgacag cgtgagccgt tccagcgggc
     7321 acttcgcctc cgggctgggc acggtcgaac tgaccgtggc gctgcactat gtctacaaca
     7381 ccccatttga ccaattgatt tgggatgtgg gacatcaggc ttatccgcat aaaattttga
     7441 ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg cggtctgcac ccgttcccgt
     7501 ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca ttcatcaacc tccatcagtg
     7561 ccggaattgg tattgcggtt gctgccgaga aagaaggcaa aaatcgccgc accgtctgtg
     7621 tgattggcga cggcgcgatt accgctggca tggcgtttga agcgatgaat cacgcgggcg
     7681 atattcgtcc tgatatgctg gtggtcctca acgacaatga aatgtcgatt tccgaaaatg
     7741 ttggcgcgct taacaaccat ctggctcagc tgctttccgg taagctttac tcttcactgc
     7801 gcgaaggcgg gaaaaaagtt ttctctggcg tgccgcccat taaagagctg ctcaaacgta
     7861 ccgaagaaca tattaaaggc atggtggtgc ctggcacgtt gtttgaagag ctgggattta
     7921 actacatcgg cccggtggac ggtcacgatg tgctggggct tatcaccacg ctaaagaaca
     7981 tgcgcgacct gaaaggcccg cagttcctgc atatcatgac caaaaaaggt cgtggttatg
     8041 aaccggcaga aaaagacccg atcactttcc acgccgtgcc taaatttgat ccctccagcg
     8101 gttgtttgcc gaaaagtagc ggcggtttgc cgagctattc aaaaatcttt ggcgactggt
     8161 tgtgcgaaac ggcagcgaaa gacaacaagc tgatggcgat tactccggcg atgcgtgaag
     8221 gttccggcat ggtcgagttt tcacgtaaat tcccggatcg ttacttcgac gtggctattg
     8281 ccgagcaaca cgcagtgacc tttgccgccg gtctggcgat tggtggatac aaacccattg
     8341 tcgcgatcta ctccaccttc ctgcaacgcg cctatgatca ggtgctgcat gacgtggcaa
     8401 ttcaaaagct accggtcctg ttcgccattg accgcgcggg cattgttggt gctgacggtc
     8461 aaactcacca gggcgctttt gatctctctt acctgcgctg cataccagaa atggtcatta
     8521 tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta taccggctat cactataacg
     8581 atggcccgtc cgcggtgcgc tacccgcgcg gcaacgcggt tggcgtggaa ctgacgccgc
     8641 tggaaaaact accaattggc aaaggcattg tgaagcgtcg tggcgagaaa ctggtgatcc
     8701 ttaactttgg tacgctgatg ccagaagcgg ccaaagtcgc cgaatcgctg aacgctacgc
     8761 tggtcgatat gcgttttgtg aaaccacttg atgaagcgtt aattctggaa atggccgcca
     8821 gccatgaagc gctggtcacc gtagaagaaa acgccattat gggcggcgca ggtagcggag
     8881 tgaacgaagt gctgatggcc catcgtaaac cagtacccgt gctgaacatt ggcctgccgg
     8941 acttctttat tccgcaagga actcaggaag aaatgcgcgc cgaactcggc ctcgatgccg
     9001 ccggtatgga agccaaaatc aaggcctggc tggcataatc cctactccac tcctgctatg
     9061 cttaagaaat tattcatggc aggagtggca gcatgcaata caacccctta ggaaaaaccg
     9121 accttcgcgt ttcccgactt tgcctcggct gtatgacctt tggcgagcca gatcgcggta
     9181 atcacgcatg gacactgccg gaagagaaca gccgtcccat aattaaacgc gcgctggaag
     9241 gtggcataaa tttctttgat accgccaata gctattccga tggtagcagc gaagagatcg
     9301 ttggtcgcgc actgcgggat ttcgcccgtc gtgaagacgt agtcgttgcc accaaagtgt
     9361 tccatcgcgt tggtgattta ccggaaggat tatcccgtgc acaaattttg cgctctatca
     9421 acgacagcct gcgacgtctc ggcatggatt atgtcgatat cctgcaaatt catcgcgggg
     9481 attacaacac gccgatcgaa gagacgctgg aagccctgaa cgacgtggta aaagccggga
     9541 aagcgcgtta tatcggcgcg tcatccatgc acgcttcgca gtttgctcag gcgctggaac
     9601 tacaaaaaca gcactgctgg gcgcagtttg tcagtatgca ggatcactat aatctgattt
     9661 atcgcgaaga agagcgcgag atgctgcctc tgtgttatca ggagggcgtg gcggtgattc
     9721 cgtggagccc gatggcgcgg gggcgactga cgcgtccatg gggagaaact accgcacgac
     9781 tggtgtctga tgaggtgggg aaaaatctct ataaagaaag cgatgaaaat gacgcgcaga
     9841 tcgcagagcg gttaacgggc gtcagtgaag aactcggtgc aacacgagca caagttgcgc
     9901 tggcctggtt gttaagtaaa ccgggcattg ccgcaccgat tatcggaact tcgcgggaag
     9961 aacagcttga tgagctgctg aacgcggtgg atatcacttt gaagccagaa cagattgccg
    10021 agctggaaac gccgtataaa ccgcatgcgg tcgtaggatt taaataagag tccaggcctg
    10081 atgagacgtg acaagcgtca catcaggcat cggtgcacaa ctacgacaga atacccagcg
    10141 gccagtgatg accgataaag tacaggatgc ctgcggaaat caccccggcg acaatatcgt
    10201 cgatcatgat ccccatgccg ccatgtacat tgcgatcaaa ccagcggatc ggccacggct
    10261 tccacatatc cagaatacgg aaaatcacaa acccggcggc aacccactgc cagtcattgg
    10321 tcggcagcgc catgagcgtg atccacatac caataaattc gtcccagaca atgctgccgt
    10381 gatcgtgcac gcccatatct ttcgccgttt gatgacaaag atagacgccg atacagatcc
    10441 ccaacatcac caccagcgag tagagctgcc agggtaaaaa ggtcatcaga taccagaaag
    10501 ggatcgccgc cagcgatccc atcgtgccag gaacgattgg gcttaatcca cttccgaatc
    10561 cgacagcaag cagatgccac ggattactca tcttcaggcg acttttcgcg acatctttat
    10621 ggcgtggcaa aatggtcata tcctttccag tcaaacgtga caggttcgcc gtcacgaata
    10681 aaacaaagcc cttcgatatc ggcggtcatt tgcccgatac aggtaaacgg tacgccaagg
    10741 tgtccgagag caacatccag cgcgccacgg ttcagttccg gcaccgtgaa acacaactcg
    10801 taatcttcac cgccagagag cgcccagcgc agcgcctgtt ccggttcaac atggcgagaa
    10861 agcacatcag aaaacggcag caatgccagg tcaatacgtg cgccgcagtc gctggctttc
    10921 acgatatgcc cgagatcgga aatcagaccg tcagagagat cgatagctga atttgccaga
    10981 tcgcgcagtg cctgcccctg taaaatacgc ggcgatggac ggagatgacg tttgatcaag
    11041 tagtcagcat ctttagcatc ggcaacctgc aaacggtttt gcaaaatcgc cagcccggcg
    11101 gcgctatcgc ccggtgtacc ggtcacatag atccagtcac ccggtttcgc cccagagcgc
    11161 gttaaggctc gtcccatcgg aacaaagccg tggataccca acgtcattga taatggccca
    11221 cgcgtggtat cgccgccaat gagttgcata tcgtaataat tgagaagatc aaacaaactg
    11281 tcgctgaagg actcaagcca cgcttcgtct acgtccggta aggttaatgc cagcgtcagc
    11341 caggccggat cggcccccat cgctgccaga tcgcttaggt tcaccgccag tgctttataa
    11401 gccagatcag caggatcgat atcagggagg aaatggttgc ccgccaccag cgtatcagtg
    11461 ctgatcgcca gggtctgttt ctcggggata ttgagaagag cgcaatcgtc gccgatgccc
    11521 agttcgacat caagacgaga acttcttaca cggtcaaaat aacgggcaat cagggagaac
    11581 tcgccacatg ccatacgtta tgcctcagca ggtaaaaaag aaaaggccgg cgatcatgga
    11641 acagtcttcc gtgaatctac cggcctggat attacttttt gttagggcga atcacaggtg
    11701 ctgctttatc gagtacgccg ttgacgaact tatggctgtc ttctgcgccg aacgatttcg
    11761 ccagttcgat cgcttcgtta atggccactt tgtatggcac atcactacgt ttagacagtt
    11821 cgtacagcgc aatgcgcagt actgcttttt ctacctggcc cagttcttcc agcaggcggg
    11881 acaggtaggg cttcatcagt ccgtcgaggt atgcggtgtt agtcgccacc ccggccagca
    11941 gctcacggaa gtacagaacg tcaacgtctt ttacatcctg ttcagccagg aactggtatt
    12001 caacatcagc gatgtcgttc tgggacaact gccaggagta gagcgcctgg acggcacact
    12061 cacgagcgcg gcgacgagca gcaggtttca cggatttccc cttactaatt tcaggccttg
    12121 atggctttca atacattaat catttcaagc gcggtcagtg cagcttctgc acctttgttg
    12181 ccagctttgg tgccagcacg ttcgatcgct tgttcaatgc tttcagtggt cagaacccca
    12241 aaagcaaccg gaatttcgct gtcctgggca acatgcgcca ggccgttgct tgcaccacca
    12301 gcgacatatt caaagtgggc agagccacca cgaataaccg tacccagcgc aatcaccgcg
    12361 tcgtatttac cggttttagc cagtgcaccc gccgccagcg gcagctcata ggcaccaggc
    12421 acccaaacaa cggtaatgtt ttcatctttt acctgaccga tacgtttcag tgcgtcaatt
    12481 gcaccttcca gcaggctgtc attgataaag ttgttgaaac gcgcgatggt gatggcgacg
    12541 cgagcgtccg gggtagcaac gttagcttca ataatgttca ttttcttcct tcgggttcga
    12601 gtatggcccc gcaggggggc ggattttagc ataatatttc gtgcgctgct tccctttcga
    12661 gccgggagat catgccccca ctaaatgcag gcaaacatcc gggcctacat gacgtatctc
    12721 tttgaattta aattgggggg cgtcggctaa tttctcaagc cctggcagcg agcataatcc
    12781 acgggcgtcg ctgcctaata gtttaggtgc gatatagaca atcagttcat cgactaaacc
    12841 cgcctgtagc aatacgccag cgagcgttgg ccccgcttcc acccagatgc tgttaatctg
    12901 ctgtttaccc agttgcatca tcagtacaac cagatccaga tgacctttat gctctggaat
    12961 cagcaaggta cgcaccgttt ccggccactc acgagaatct tcctgcgtac gcgcgaacca
    13021 ggtttcgccg ggctgctgca caatgcgatg ttccggcgtc acgcgatttt ggctatcaat
    13081 cacaatacgt actggctggc ggagattttg ttgcgggtag agcgcctgag tttgttcatc
    13141 cagttcagac caacgcactg ttaaggctgg atcatccgcc agcaccgtgg cgctgctggt
    13201 taaaatggca tgactttgtg cgcgctgtcg ttgtacgtcg cgccgcgcct gaggcgaagt
    13261 gatccactgg ctttcgccgc tcgccatcgc cgtgcggcca tcaagcgatg cgccaagttt
    13321 taactgaata taaggaaagc cggtgcgcat ccgcttgaga aagcctttat tcaattgctc
    13381 ggcttcactc atcatcagcc cgtggctgac gtcaatgcca gcctgttgca gacggtaaag
    13441 tccacgccca gcgacctgcg ggttaggatc ttgcatcgct gcgaccacgc gcgctacgcc
    13501 agcggcgatg agtgcgtcac agcacggtgg cgtacggcca tgatggctac agggttcgag
    13561 tgtcacatac gccgttgcgc ctttggcttt ttcacccgcc atacgcaacg cgtgtacttc
    13621 ggcatgtggt tcacccgctc gttggtggta accttcaccg acaatttcgc catctttgac
    13681 aatgacgcac ccgacattcg ggttggggtg ggtggtaaaa cgtcctcgtt gcgccagctt
    13741 tagcgcccgc gccatgtaat actcgtcctg cacggcttag tcctccaggc gcgcgatctc
    13801 ttcgccaaat tctttgatat cttcgaaact gcggtagaca gaggcaaaac ggatataggc
    13861 gactttatcg agctttttca attgctccat caccagattg ccaatcatct tgctcggtac
    13921 ttcgcgctca ccggtggcgc gcagctgcga tttaatatga ttgattgcca tttcaacgta
    13981 atcggaactc accggacgtt tttccagcgc ccgcagcatt ccgctacgca atttctcttc
    14041 attaaacggt tcacgcacgt cgttgctttt tacaacacgc ggcataacca gctccgccac
    14101 ttcaaaggtg gtgaaacgtt cattacacac cagacactgc cgacggcggc gtacggatga
    14161 accctcgccc acgagacgag agtcaattac cttagtgtcc acggcgaaac agaatgggca
    14221 atgcatacgg cgtcctgacc aggtggttaa cggaaatcta ttttaccctg aactgacgat
    14281 acaacaaagg cgcaacgctt ttgagataaa ggatcgatgc tttcgctgct tttaccctct
    14341 accattaggc taattgcgac gagaaaggga cgacctatga atactaacgt ttttcgactg
    14401 ctcctgctgg gaagcctgtt cagccttagc gcctgtgtgc agcaaagtga agtgcgacag
    14461 atgaaacaca gcgtcagcac gctgaaccag gagatgacgc aactcaatca agaaacagtc
    14521 aaaatcactc agcaaaacag gctgaatgca aaatccagca gtggggttta ccttctgcct
    14581 ggagcgaaaa caccggcaag actggaaagc cagatcggta ctttacgtat gtcgctggtg
    14641 aatattacgc ctgatacaga tggcaccacc cttacactac gtattcaggg tgagtcgaat
    14701 gatcccttgc ccgcattcag cggaactgtt gaatatgggc aaattcaggg aacaatagac
    14761 aactttcagg aaatcaatgt gcagaatcaa ttgattaatg cccctgccag cgtcctcgcc
    14821 cccagcgatg ttgatattcc gttacaatta aaggggattt ctgtggatca gttaggcttt
    14881 gtacgtatcc acgacattca acctgttatg cagtaaacgt attttgggac gattttgtgc
    14941 gtcccgcaac atctttcccc gtcattttgt tactctgctt acatcacttg gattgatagt
    15001 aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt gtaaacgtga
    15061 acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg tgagcaatga
    15121 tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc gcctttttca
    15181 ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac tggcagccgg
    15241 tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg acaaaccgca
    15301 gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc acacccgttt
    15361 cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta aaaaagactg
    15421 gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact ccgatgctaa
    15481 aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt tctccatcga
    15541 caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact tcgcgaacaa
    15601 ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt acatgggtct
    15661 gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct atgcgaaata
    15721 ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc gtttcaaaat
    15781 taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca tcggcttcac
    15841 caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca acggtattaa
    15901 gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact acgatcactg
    15961 gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg acgatgcaga
    16021 actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg gttacctggt
    16081 agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat ttctgattgt
    16141 taacaacatg tgctttgttc atgccggatg cggcgtaaac gctttgtccg gcccacaaaa
    16201 gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag cgcatcaggc
    16261 aattttacgt tttaatcact tcttcttgtt catcatcgct ttcagatcag cgaatgggtt
    16321 gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg tcgtaccata
    16381 ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac acaacaattc
    16441 ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt gaacggtaag
    16501 ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc acggatagat
    16561 ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg ggatgatagc
    16621 catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga tgcggggatt
    16681 ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt tcaacatcaa
    16741 cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc cactttctgc
    16801 tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc tacgtagatg
    16861 gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc ttccagtacc
    16921 ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga tgtgatcaag
    16981 gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt accgcgacgg
    17041 atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt aagcgagtaa
    17101 ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg gaataacgac
    17161 aaaataccca gcgtaatgat aacgtcgtgc gccagcgcaa taaccacccc tgccgccagt
    17221 cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc catcaacgcc
    17281 atcgcaccgg tttgcgcaag gtctgccccc acgctcggac cgacgaactc aatacgcttc
    17341 actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct gcccagcacc
    17401 tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg gctgctacca
    17461 aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg cattacgtca
    17521 atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa atccagcccc
    17581 cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag accagagatg
    17641 ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg gtttagttgt
    17701 tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct tgacgcgctt
    17761 gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa acatcgacgt
    17821 cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac ccactgcgta
    17881 caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac tgaatgcgcc
    17941 acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact cttctttaat
    18001 acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga cgatacccgc
    18061 gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga cgatcaagat
    18121 caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga agatgatcat
    18181 gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct caatgttctg
    18241 catacccagg gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg cgatcaacgc
    18301 accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga tgccggtgat
    18361 acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct cttcctgttt
    18421 caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact ccacaaacag
    18481 ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt tgccaccagc
    18541 gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg aagtggagtc
    18601 ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct gaccttcgcg
    18661 ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg cctgatcaac
    18721 attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct ctttcgcacg
    18781 cgcagtgtcc tgaataccag gcagttcaac aacgatacgg tcagcaccct gacgctgaac
    18841 caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa tgttctgctg
    18901 caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac gcagctggtt
    18961 gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg caatggcttc
    19021 atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt ctttacgaac
    19081 agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga tattttgttc
    19141 ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa cgccgccacg
    19201 caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc gcggcgttgc
    19261 cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta atgcttcacg
    19321 agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct cttccagtgc
    19381 cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga tcagcgtttg
    19441 ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct caccaaacag
    19501 gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga cgtacttcca
    19561 caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt acagcgcctt
    19621 catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta cttcagtggt
    19681 gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc gaccaaccag
    19741 gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct ttttgtgttc
    19801 tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac cgaataccac
    19861 cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg ccgctaccgc
    19921 atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca acgttcaaag
    19981 gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct aatttaccct
    20041 cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg ttatgaatgg
    20101 tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag taagcgcgtg
    20161 aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta tcgctcttat
    20221 atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca tttcgggcgt
    20281 tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct tcaaccaggt
    20341 cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt tgcggacaca
    20401 catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga ccgccgacag
    20461 cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt aaatcttcgt
    20521 aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg tcaaaacgct
    20581 cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg taatcccagt
    20641 cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca agatcgtact
    20701 gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc gggttacgga
    20761 agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc tggaagccgc
    20821 cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcatatcg ccgtgcagtt
    20881 tcatgatttc ctggcccggg cgcagccaca ggtggaaagt gttgccgagg ataatttgcg
    20941 cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag gtgccaacag
    21001 gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca cggcgtgcgc
    21061 gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag aaaaacagtc
    21121 caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt gcctgcggat
    21181 tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct tctaccgccg
    21241 ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc ataatcagcg
    21301 tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac tggaagcccg
    21361 gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt tttgccgcct
    21421 gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc cggttaccgc
    21481 gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg tattccgagt
    21541 gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc gcaccaacgt
    21601 gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc aaaggctcgt
    21661 caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta taaacggttt
    21721 gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc agcggcatat
    21781 ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg acttcaaaca
    21841 gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc agcaacagtt
    21901 ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt ttgtcgtcga
    21961 gcatccgctc aaccagcact tcaatcttac cgccgctggc tttacgccca aacagacgcg
    22021 ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc ttatcgagta
    22081 aatcggtgaa agtaccgtgc gtcagcgcac ccgtcggccc gtccagcgac agtaaacgac
    22141 agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat tcaaaggaga
    22201 aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag tctagtgccg
    22261 gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat gaatttttta
    22321 gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt actggctgat
    22381 ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg cattcatatg
    22441 catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacgggagt ggtttcgtag
    22501 tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact ttctttcccg
    22561 ccactggtcg cagctgtcgc cggactttcc actacaggaa tttgtctgtt atgcccgcga
    22621 gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga acaattactt
    22681 gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga acgtgttaaa
    22741 cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt acgatttaga
    22801 cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga tgatggcgca
    22861 ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga aatggcaggc
    22921 aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca ttgcacggca
    22981 ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt gattgccacc
    23041 agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc cgcatacagc
    23101 acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat cattcgccgg
    23161 tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg tttacggcaa
    23221 gacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca cggtacacca
    23281 ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg accgagcagc
    23341 gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa ttgttgttga
    23401 tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc gtcaatttgc
    23461 tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa tgtgaagcca
    23521 cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa
    23581 tgttcgccga caatatacgc ttccgactgg gtttctttcg ccgcttcggt gatcccggca
    23641 acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac cacatccagc
    23701 cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact gtcttccccg
    23761 cgataaattt cattcaccag actttccgac tgataatcca gcttcggcaa gctggcatag
    23821 ccaagccagt cgagcgccgt gccatcatca ctaaacaagt accagtcgcg ccagggcgat
    23881 tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtca
//
LOCUS       DAJTNM010000054        23541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTNM010000054 DAJTNM010000000
VERSION     DAJTNM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.054"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="OO172_002226"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="OO172_002226"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5512404.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="OO172_002227"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="OO172_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5512405.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="OO172_002228"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="OO172_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5512406.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="OO172_002229"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="OO172_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5512407.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3874..3998
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3880..3924
                     /gene="pheM"
                     /locus_tag="OO172_002230"
     CDS             3880..3924
                     /gene="pheM"
                     /locus_tag="OO172_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5512408.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="OO172_002231"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="OO172_002231"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5512409.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="OO172_002232"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="OO172_002232"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5512410.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRIAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="OO172_002233"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="OO172_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5512411.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="OO172_002234"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="OO172_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5512412.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="OO172_002235"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="OO172_002235"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5512413.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="OO172_002236"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="OO172_002236"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5512414.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="OO172_002237"
     CDS             10418..10882
                     /locus_tag="OO172_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5512415.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="OO172_002238"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="OO172_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13298
                     /locus_tag="OO172_002239"
     CDS             11895..13298
                     /locus_tag="OO172_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5512416.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPD"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="OO172_002240"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="OO172_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5512417.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="OO172_002241"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="OO172_002241"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5512418.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="OO172_002242"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="OO172_002242"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5512419.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="OO172_002243"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="OO172_002243"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5512420.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="OO172_002244"
                     /pseudo
     CDS             complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="OO172_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="OO172_002245"
     CDS             complement(20164..20457)
                     /locus_tag="OO172_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5512421.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="OO172_002246"
     CDS             complement(20454..21743)
                     /locus_tag="OO172_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5512422.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFIYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21799..22737)
                     /locus_tag="OO172_002247"
     CDS             complement(21799..22737)
                     /locus_tag="OO172_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5512423.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIISLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTQGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22757..23521)
                     /locus_tag="OO172_002248"
     CDS             complement(22757..23521)
                     /locus_tag="OO172_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5512424.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5796 a         5751 c         6203 g         5791 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggtt ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agtatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatagcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtggc
    11401 tacattatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcaacaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgagg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaaccaata cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagataa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 cccctgcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggaaatga tcgccagcgg
    22321 gctgttgatc ttttcctgag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat
    22381 gtgaccatcg acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac
    22441 acttaaccgt gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg
    22501 gtgtttatct ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc
    22561 gtcgttttgc tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg
    22621 gtcggtattt tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag
    22681 ttcagcataa cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt
    22741 ttcttctccg taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg
    22801 cctccggcga atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa
    22861 tgccgaccag ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact
    22921 gatttaccgg ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt
    22981 taatatcgga ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat
    23041 cttcaagcgt gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat
    23101 taatcaccgg aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt
    23161 cgccggaacc ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca
    23221 gcgcttttgc ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca
    23281 ggctgtgcgg cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc
    23341 cgccaacggt cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa
    23401 tggcattgag atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt
    23461 ctggagtgac aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca
    23521 tgatttctcc tgtgagtcgc a
//
LOCUS       DAJTNM010000055        23500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTNM010000055 DAJTNM010000000
VERSION     DAJTNM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.055"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(35..1399)
                     /gene="tdcG"
                     /locus_tag="OO172_002249"
     CDS             complement(35..1399)
                     /gene="tdcG"
                     /locus_tag="OO172_002249"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5512425.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALHRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1471..1860)
                     /locus_tag="OO172_002250"
     CDS             complement(1471..1860)
                     /locus_tag="OO172_002250"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5512426.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1874..4167)
                     /gene="tdcE"
                     /locus_tag="OO172_002251"
                     /pseudo
     CDS             complement(1874..4167)
                     /gene="tdcE"
                     /locus_tag="OO172_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(4201..5409)
                     /gene="tdcD"
                     /locus_tag="OO172_002252"
     CDS             complement(4201..5409)
                     /gene="tdcD"
                     /locus_tag="OO172_002252"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5512427.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5435..6766)
                     /gene="tdcC"
                     /locus_tag="OO172_002253"
     CDS             complement(5435..6766)
                     /gene="tdcC"
                     /locus_tag="OO172_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5512428.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMAGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6788..7777)
                     /gene="tdcB"
                     /locus_tag="OO172_002254"
     CDS             complement(6788..7777)
                     /gene="tdcB"
                     /locus_tag="OO172_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5512429.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAILGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7876..8814)
                     /gene="tdcA"
                     /locus_tag="OO172_002255"
     CDS             complement(7876..8814)
                     /gene="tdcA"
                     /locus_tag="OO172_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5512430.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            9004..9348
                     /gene="tdcR"
                     /locus_tag="OO172_002256"
     CDS             9004..9348
                     /gene="tdcR"
                     /locus_tag="OO172_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5512431.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            9604..10143
                     /locus_tag="OO172_002257"
     CDS             9604..10143
                     /locus_tag="OO172_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512432.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            10165..11341
                     /locus_tag="OO172_002258"
                     /pseudo
     CDS             10165..11341
                     /locus_tag="OO172_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
     gene            complement(11951..12327)
                     /gene="rnpB"
                     /locus_tag="OO172_002259"
     ncRNA           complement(11951..12327)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO172_002259"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(12360..13511)
                     /gene="garK"
                     /locus_tag="OO172_002260"
     CDS             complement(12360..13511)
                     /gene="garK"
                     /locus_tag="OO172_002260"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5512433.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHTWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(13608..14498)
                     /gene="garR"
                     /locus_tag="OO172_002261"
     CDS             complement(13608..14498)
                     /gene="garR"
                     /locus_tag="OO172_002261"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5512434.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(14528..15298)
                     /gene="garL"
                     /locus_tag="OO172_002262"
     CDS             complement(14528..15298)
                     /gene="garL"
                     /locus_tag="OO172_002262"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR5512435.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(15314..16648)
                     /gene="garP"
                     /locus_tag="OO172_002263"
     CDS             complement(15314..16648)
                     /gene="garP"
                     /locus_tag="OO172_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5512436.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            17023..18594
                     /gene="garD"
                     /locus_tag="OO172_002264"
     CDS             17023..18594
                     /gene="garD"
                     /locus_tag="OO172_002264"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5512437.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(18646..19470)
                     /gene="agaR"
                     /locus_tag="OO172_002265"
     CDS             complement(18646..19470)
                     /gene="agaR"
                     /locus_tag="OO172_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5512438.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            19719..20519
                     /locus_tag="OO172_002266"
                     /pseudo
     CDS             19719..20519
                     /locus_tag="OO172_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
     gene            20519..20983
                     /gene="agaV"
                     /locus_tag="OO172_002267"
     CDS             20519..20983
                     /gene="agaV"
                     /locus_tag="OO172_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5512439.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            20994..21773
                     /locus_tag="OO172_002268"
     CDS             20994..21773
                     /locus_tag="OO172_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5512440.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            21763..22572
                     /locus_tag="OO172_002269"
     CDS             21763..22572
                     /locus_tag="OO172_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5512441.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            22659..23093
                     /locus_tag="OO172_002270"
     CDS             22659..23093
                     /locus_tag="OO172_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5512442.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            23090..>23500
                     /locus_tag="OO172_002271"
     CDS             23090..>23500
                     /locus_tag="OO172_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5512443.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAH"
BASE COUNT         6121 a         5994 c         5544 g         5841 t
ORIGIN      
        1 tttgtgcacc caaggatgaa agctgacagc aatgtcagcc gcagaccact ttaatggcca
       61 gtcctccgcg tgatgtttcg cggtatttat cgttcatatc tttgccggtt tcatacatcg
      121 tctcgatcac tttatcgagt gaaacacgcg gtgccgaggt gcggcgcatc gccatccgcg
      181 cggcgtttac tgctttcacg gcattaatgg cattacgttc aatgcacggg atttgtacct
      241 gtccggcaac cggatcgcag gtcagcccaa ggttatgctc catcgcgatt tccgccgcat
      301 tgcatacctg cgccggactg ccgcccagta gttcagttaa ccctgccgct gccattgaac
      361 aggccacgcc aatctccccc tgacagccga cctccgcgcc agagatggag gcgttcattt
      421 tatacagcgc gccaatagcc cccgctgcca gaaaatagcg ggcaattgac cgctcgttta
      481 ccggacgacg gaacttatcg taataagcca gcactgccgg aataatgccg cacgcaccgt
      541 tagtcggtgc cgttaccacg cgcccgccag ctgcgttttc ttcactaacc gccagcgcgt
      601 acatgttgat ccagtcgatg acattcatcg gatcgttaga gatgttatcg ctagaaacca
      661 gctgacgatg cagcgctacg gcacgacgcg gcacattgag cggaccaggc agcacgcctt
      721 cagtgttcat cccgcgttca ataccgtcat gcatcacttg ccagatacgg gcaaaccctg
      781 cgtcaatttc cgctttgctg cgcagcgcca gctcgttgtg catcatcagg ccagatatag
      841 acaggccgtt gtaatcacac attttcagca gttcacctgc tgagtggaaa tcgtaaggta
      901 cggacgtttc gacatcgtgc gacaggccga agtgttcttc ttcgacaata aacccgccgc
      961 cgacagagta ataggtttta cttaatagcg cttcctgtcc cttccaggca gtgatccgca
     1021 ttccgttctc atggcgaggc aacatttcgg gatggaagat aatgttcttt gctacaggaa
     1081 aatcaacaat atgcgcacca gatgccactg gcagccgccc gctgcgcgtt actaactcta
     1141 taaatgcagg gatctcatca atgacaacat cctgcggact gtttcctgcc agtcccatga
     1201 taatggcgac atccgtggca tggcctttgc ccgtcagtga caacgacccg tacagatcga
     1261 ccacaatatg gctcgtcgcg gttaataagc cgctactttc cagccgatca ataaaacttt
     1321 ttccggcatt cattggcccc acggtatgcg aactggaggg accaatccca attttgaaaa
     1381 tatcgaatgc actaatcata tccacaccct cggattgccg ttcagtgaag tggagcggaa
     1441 cgaccttgcg accgtcccgc tcacgaggct ttacgcacta cgtactgcga tagcttcaat
     1501 ttccagcttc acatctttcg gcaaacgcgc aacctgcaca cagctccggg tcgggtaggt
     1561 cgcctgatgc tcatcgaaga actgcttata gacttcattg atggtggcaa aatcattcag
     1621 atcggtgatg aacacggtca tcttgatgat atcgcccacg ctcagcccgg cagcaaccac
     1681 gatcgctttg acgttttcga ggcttaaacg cgcctgatct tgcacatcag ccgggatctc
     1741 accggtctgc gggcaaaccg gaatttgccc ggaggtgaag accatgctgc ctaaatcaac
     1801 gccctgaaca taagggccga ttgcgcctag ggcacgttgc gtttcgataa tctttttcat
     1861 acatcctccg gcgtcagagc gcctgggtaa aagtacgtga aataacatcc tgttgctgtt
     1921 cacggttcag tgcgttgaag cgcacggcgt agccagagac acggattgtc aggttaggat
     1981 atttttccgg gtgctcgatg gcatccagca gcatttcccg attcattacg ttgacgttga
     2041 ggtgttgacc gccttcgaca tccgcttcgt ggtggaaata cccatccagc aggccgacaa
     2101 ggttggtttt acgtactgga tcttctttgc ccagcgccgc aggaacgatt gagaaggtgt
     2161 acgagatccc atctttggcg taggtgaacg gcagtttcgc caccgacgtc aatgaggcca
     2221 cggcaccttt gcggtcacga ccatgcatcg ggttagcgcc cggcgcgaac ggtgttccgg
     2281 cgcgacgacc gtccggcgta ttaccggttt tctggccgta caccacgtta gaagtgatag
     2341 tcagaatcga ctgggtaggg acggcgttgc gataggttgg cagcgcttta attttcttca
     2401 taaagcgttc aaccaggtcg caggcaatgc tgtctacgcg ctcgtcgttg ttgccgtact
     2461 gcggatattc accgtcgatt tcaaagtcca ccgccaggcc gttttcgtca cggattggtt
     2521 tcacgcgggc atatttgatg gcagacaggg agtccgtcgc caccgacagg cccgcgatgc
     2581 cgcatgccat agtgcgatag acatcacgat cgtgcagcgc catcagcgaa gcttcgtagc
     2641 tgtacttgtc gtgcatgtag tgaatgatat tcagcgcgct gatgtactgc accgccagcc
     2701 agtccatgaa gtgatcgagg ctgtccatca ctttgtcgta atccagcacg tcgtccatca
     2761 gcggtgctgt tttcggcccg acctgaatct tcagcttctc gtccaccccg ccgttaattg
     2821 cgtagagcag cgttttcgcc aggttagcgc gtgcaccaaa gaactgcatt tgcttaccaa
     2881 tcaccattgg gctgacgcag caggcaatcg cgtaatcgtc gctgttgaag tcagtacgca
     2941 tcagatcgtc attttcatac tgcaaggaag aggtgacgat cgacacctgc gcggcatatt
     3001 ttttgaaggc aatcggtaat tcttccgacc aaagaatggt caggttaggt tccggtgccg
     3061 gccccatagt gtgcaggtgt gcaaatagcg gaaggagttt ttggtcacca gcgtacgacc
     3121 gtccagcccc atcccgccga tcacttccgt cgcccagatt gggtcgccgg agaacagcga
     3181 atcaaattcc ggtgtacgca ggaagcgtac catacggatc ttcatgatga agtgatcgat
     3241 cagttcctgt gcctgctgct cattgaggac gccagcttta aagtcgcgct caatgtagat
     3301 atccaggaac gatgccgtgc gccccagcga catcgcgccg ccattttgcg atttcactgc
     3361 cgccagataa gcgaagtaga gccactgcac cgcttcctgt gcattctgcg ccgggcgaga
     3421 gatatcaaag ccatatttcg ccgccatttc ctgaatctgc aacagcgcat gacgatgctc
     3481 tgccagctcc tcacgcagac ggatggtggc ttccagatcc tcgccttttt ccagacgaga
     3541 ctggagatcg gcaaattgca gttcgcgttc acgcaccaga taactgatgc catacagcgc
     3601 tacgcggcga tagtcaccga taatgcgccc acgaccatag ccatctggta aaccggtcag
     3661 cacgccagat ttacggcagc gcagcatatc cggtgagtaa acatcaaata cgccctggtt
     3721 atgggtttta cgcagatcgg taaacagata ttcaaattca ctgtccattt cacggccata
     3781 ggcgtggaat gaacttttaa tcatattaat gccaccgaac gggtgtagcg cacgtttcaa
     3841 cggcgcatcc gtttgcaggc caacaatttt ttccagcggc tggttaatat atcccgcatc
     3901 atgagcggta attgtggtgg caatattggt atcgaaatca accggcgcgt gggttgcgtt
     3961 ttcgatacgg atgccttcca ttactttttc tcacaattcc gtggtggcag gcgtcgcttc
     4021 ggcgagaaaa gattcatcgc cttcatacgg tgtatagtta tgttgaataa aatcgcggac
     4081 attaatttcg cttttccagt ccgtaccttt aaagccaagc catgcgtcag cgtacagctt
     4141 atcgctggta tcaatatcta ccttcatgaa aaataatctc tctacaatac ttcaactaaa
     4201 ttatgcaaat tctgcgggcg cgttaacttt acctaaatga atggcatcca aagcaatcat
     4261 tttttcttcg ttagtcggaa taacgacgca aatgacatgc gcattttcac tggaaacaat
     4321 tcgctcgcca aaagagttag atcgattatt catttctgta tcaatctcta cgcctaatac
     4381 agccaaatgt tccatgacca gacgacgaat taaacttgaa ttctctccta ttccgccggt
     4441 gaatataatt ccatccagcc gacgtaatga agctgcatgt ccggcaatat gacgggcaat
     4501 tcggtgaaca aaggttttaa ttgccagttg cgcgcgttcg tgaccttcat gccaggcttt
     4561 ttccagaaca cgtaaatccg aagaaagacc ggaaatacct aataatcccg actctttatt
     4621 cactacgcgt tccaggtcac ccaggctctg gttggtttgg ctggcgaccc aggacatcgc
     4681 accaaagtcg acatcgccac tgcgggtacc catcatcaag ccttccagcg gcgtcattcc
     4741 cattgaggtg tcaacactct gaccgttgcg aactgcgcag attgacgcgc cattgccaag
     4801 atgcgccaca accaggccgg agtcatcttc cgccagattc agcagcgaat gtgcgcgctg
     4861 ggaaacatag cggtgcgacg tgccgtggaa accataacgg cgtacaccta actcttcata
     4921 atatttccac ggcaggccgt ataaataagc ttccggagcc atcgtctggt ggaaactggt
     4981 atcaaatacg gccacctgag ttacgcccgg aaataattgc tgcgccgatt caataccact
     5041 taaatttgcg taattatgca ggggtgccag tggagaaacg cgacggatat tatcaatgac
     5101 ttcatcggta ataatggcgg actcggtaaa aatactgccg ccgtgagcga tgcggtggcc
     5161 aattaaggcc acgctgtcat ttaaattccg tttttccagt tcaaatgcaa ttgccttcaa
     5221 tgcaccttcg tagctgtgga gagccagcgg tgctggctct cccccattta cggataagaa
     5281 tgcattttcc gagttaatac cgtcggcaat acctgacatt aatacttcac agtcgctggc
     5341 atcgagtacg gaaaacttaa tcgaagacga accacagtta ataaccaaaa caaccggaaa
     5401 ttcattcatc tcttttctca tcctgagtta cggattaaaa cagtttgtat acgatgttca
     5461 ggatggtcag cagaccaatc acggtaacaa acacgttatc cagacgacca cggtatttcg
     5521 ccagagacgg cgctttacgg atagcataca tcggcaacag gcacagcagg gatgcgataa
     5581 tcggtgcgcc catggcttca atcaggtcga ggatgttcgg gttggcgtag gcaacaaccc
     5641 aggtggagcc catgatgaag atcatgctga gagtattcag tttacccagc gacactttgg
     5701 ttttgtcacc tttataaccg aacttcagaa tcagaccatt caagccttcc agcgtcccca
     5761 gatagtgacc gaagaaagat ttgaagatag ccacgagtgc gatgatggaa gccgcatatt
     5821 ccagtgtaat cgcgaacgtt gttttcgtac cggccatgga cgcaaagtgg ttagccagat
     5881 aagaaagcac tggaatattc tgcactttgg cttccgccat gttggccgga gacagagtaa
     5941 acaggcagct aaaggcaaag aacatcacca ctgcaaccat cagcatgctg gcacgagaaa
     6001 tgatttggga acatttacgt tcggtgaagt cgcgaccgaa gtctttctca tactcttcac
     6061 gtttagaaac cacgaaggaa gagacgattg gcgagaagtt aaaggagaaa accatgatgg
     6121 aaatccccag ccagacagtg atcaggatac cgtcatgacc ggttaacgac agcgaaccga
     6181 ggtcaacctg gtcgataact gcagagttcc agtaagggat cagcgacaga gaaatcagca
     6241 ccaggctggc gataaacggc cataccaggt agctcatcac tttaaccatc agatccttac
     6301 caaaccagat gacgaaagcc atcagcagca acaggaacag cgccacaaag ccgcgattca
     6361 gcggtgcaaa tccgagctgg ttttcccaga acgtcataaa ggtattggta atagtaacgc
     6421 cataaatcca cagcagtggg caaatcgcga agaagtacag gaacgtgata accacgccgc
     6481 cagttttacc aaaatgctct tccaccgttt ccgtaatgtt gccggaaggg ttagagccag
     6541 aaagacacag acgcgccagc gcccggtggc aataaaatgc gatggggtat gccaatacca
     6601 acatcagaag aatcgggatc agtccgccaa aacctgcgcg gatagggaag aacagcaccc
     6661 cggcgccgat tgccgtacca aacaagccta acgtccatgt ggtatctgat ttacgccagg
     6721 acgattgttt tgtctggctg gatacaatgc tatctgaagt actcatatcc tatcctcaac
     6781 gaattaatta agcgtcaaca aaaccggtga tttgagagac gcgagaaaga tcgatattgc
     6841 cgccggaaat aatactgacg gtttttctgt tttgaatata ttggtctaat ttaccgctta
     6901 ataatgcagc acatgccaga gcgcctgcgc cttcggtgac gactttattg cgctgaatta
     6961 aggcaatcat actgtttctg atttcgtctt cgctgaccag cacgatgtca tcgactaatt
     7021 cacgaacgat ttcgtaagtt aaattacccg ggcgggagac atcacaacca tccgccaggg
     7081 tgccggtagt tcggtgcgtg gttatttctc cggagtggaa agaagccgcc atgccgtgaa
     7141 cgttttctga ctgtacgcca ataacacgaa tggtcgggtt aatagattta attgccaccg
     7201 caataccagc aattaaacca ccaccaccaa ttggcacaat cacgttatcg acatcataga
     7261 gatcttccat aatttccaga ccaatcgttc cctggccagc aatcactttc ggatcatcgt
     7321 aaggtgggat aaaaatacga ccttccattt cgacaatttc gctcacttta gcgatagtgt
     7381 cgttgaagtt atcaccatgc agaacgactt ctgcggagta gtcgcacgtt gccgctactt
     7441 tggattttgg cgcacctttt ggcatcacca ctttaccgtc gatacccagt atcgcgcagg
     7501 agagcgaaac cccttgcgca tggttgcccg cagaacaggc caccacgcct ttgcgttttt
     7561 ccgcatcggt cagtgaactt aatttattaa atgcgccacg aattttaaat gaacccgtac
     7621 gctgcatatt ttcaaacttc aggaatattt cacctttgca acgttcacta aaatagttgg
     7681 agcgaggcat gcctgtttta taaattcgcc cagccagtcg ttgtttcgct tcaataatat
     7741 catcaatagc aaccggcaga tcgtatgtaa tatgcattat aaaacctctt cgcctgaatt
     7801 aaatatgtag gtaaccgtaa ccgacacctg caaaacagac aggtggatta tttatgttta
     7861 aataaaaaca aataactaac caacttctat taattgcctt cgtctacacc cattataaga
     7921 tgaatactct ttggctaatt ccaccaaaac cgatgctgct tttttaatac gataattttt
     7981 cgaccatacc gcggcatatt gtgccacagg taatgtttct tcaaccggaa tagtaataaa
     8041 ttgattagaa ccaaaaggtg acgtcatatc acaaggaatt acagttaaga aatcagcatt
     8101 gagaacaaga ttataaattg tcacgactga gtcggtttta acgatgtttt caatactgat
     8161 gccatttctt tgtaacgtag taagcagttc gctgtagtac cccatattag tttgtggcaa
     8221 cacccactgt tcgttcttca acgactccag cgtggtggtg ccggtgcatg ttcgggactt
     8281 actggctacc agcacaaact cggactcgaa cagcggctca acatgtaaat cctgaagctt
     8341 catttctgca cttaacgtac caatcgcaaa atccagtcga ccgtcgcgga ttgccggtaa
     8401 gaacgaagac agttgcgctt catacataga aacctgcgct ttcgggaaca cctctttgaa
     8461 cttgttgatc atccctgaca taaaagtaaa accaatcaat gaaggaaaac caaatgagac
     8521 ttccaccacc gcctcagaag acataccgct tatctcatta accatatttt tcatttcacg
     8581 ggtaatggat tcggaacggg agagtaacaa ttgaccggca ggtgttaatg ttacaccggt
     8641 atttttccgc accactaatt ccacaccaaa ataatcttca atatcgttaa tgattttact
     8701 gacggccggt tgagttaacc ctaattcttt tgcagccgag ccgatagagc cacttctaat
     8761 gacttcctga aagactacca ggtgctgcgt tttcggaaga agaatagtgc tcataacgac
     8821 ctacgttaat tacctcattg acggcatgaa gtgtatcaaa atgaaataaa caggatatgt
     8881 gcgaccactc acaaattaac tttcattact ttccaaggta tcgttattac aattaaatac
     8941 ctttaaatat caacaagtta aagcataaaa atcagcataa accctgattt ctatcaaaaa
     9001 aaaatgacgg ggataaccat attttatggc gataacatca ttcgttatgt ggttaataca
     9061 aaaaaggggc tgagaccata tttcaagcaa ttaccggata attaccaggc aaaatttgag
     9121 ttaaatttaa tgagcaagtt ctcaaatttt attataaata aaccattttc agcgataaat
     9181 actgcggcac gtcacatttt ttcacgctat ttgttggaga acaaacattt attttatcaa
     9241 tattttaaaa tttcgaatac aggtattgat catctcgaac aattgattaa cgtcaacttt
     9301 ttctcttctg acaggacgtc attttgtgaa tgcaatcgtt ttccataaat tcttctcccc
     9361 tcataggcga cgaatagcat tttgtgttga ggatcacaaa acgaataatt gctggtcgcc
     9421 gcgataaggt cagacgaagt caacaagggg gattttcact gtgctttaca tcagcgtagg
     9481 acacagaatg ttgcttcgtt tactgtaacg ccgggtaaat gagcgttttt tgatagtgcg
     9541 taagtgtcgt gcgaggaaaa taagcatcta ttattgtttg tcatgacgac aaggcaggca
     9601 tcaatgaaag gcttcccaat tgcacatatt tttcaccctt caatcccgcc aatgcacgca
     9661 gtggttaaca atcacaatag aaatattgat tattggacgg taaaaagaaa gtttgcagaa
     9721 attgtctcca ccaatgacgt taataaaatt tacagtataa gtaatgaact gcggagagta
     9781 ttatctgcaa ttactgcatt gaatttctat caaggcgatg ttccttctgt catgatccga
     9841 atccaaccgg aaaatatgag tccattcatc atagatattt ctacaggaga acatgatgat
     9901 tatatcatac aaacattaga tgtaggcact tttgcacctt ttggtgaaca atgtacttgc
     9961 tcagccgtca ataaaaaaga gctggaatgt attaaggaga cgatttctaa gtattgcgca
    10021 aagttcaccc aaaaagaagc catcttaaca ccccctaggc actttaataa aacctgcatt
    10081 acttcagatt gttggcaaat tctctttttt tcacccgatc attttaataa tgatttttat
    10141 tgattattta taaaggataa aataatgttc cctgtttcat caattggtaa tgatattagt
    10201 agtgatttag tccgtcgaaa aatgaatgac cttcctgaaa gccccatagg aaataacctc
    10261 gaagcactag cacccggtat agaaaaacta aaacagacct ctattcaaat ggtcacttta
    10321 cttaacgcgt tacaaccaag tggaaaatgc attatcactg gtgattttca aaaagaatta
    10381 gcgtacttac aaaatgtaat tctttataat gactcgtctc ttcgtctgga ttttttaggt
    10441 tataacgccc aaattattca acgatcggac aatacctgcg aacttaccat taatgaaccg
    10501 ttaaaaaacc aggaaatatc tacaggcaat atcaatgtta attgcccatt aaaagatatt
    10561 tacaatgaaa tcaggaggtt gaacgtaatc tttagttgtg ggactggaga tatagttgat
    10621 ctatcctctc tggacttacg taatgtcgat ttagattatt atgatttcac agataaacat
    10681 atggctaata ctattctaaa tcattttaaa ttggattcaa caaattttac taatgccaac
    10741 atgtttcagg ttaattttgt tagttcaaca caaaacgcca caatctcctg ggattattta
    10801 ctaaaaataa cgcctgtttt aataagcatt agcgatatgt attctgaaga aaaaatcaag
    10861 tttgtcgaaa gttgtttaaa tgagcttgga gacattaccg aagaacaatt aaaaattatg
    10921 agatttgcaa ttataaaatc tataccaagg gcaactctta cagataaatt agaaaatgaa
    10981 ttaacaaaag aaatatataa aagctcatcg aaaatcaaca attgcttgaa cagaattaaa
    11041 ttaccagaga tgaaagaatt ctcatcaaaa aaatatatga ttacatcgat acaatcattg
    11101 aagattatga aaatatcaaa gaaaatgctt atctggtcgt cccccaaatt aattatacta
    11161 tggatttaaa catagaagac tctagctcag aagagttaat accctcgaga aagacgaaaa
    11221 ttctccggac aatggctttg aggtcgggga atataacaca tatgaagcat ataactcaga
    11281 gaatcaatat tttaccagag aggactatac gtataattac gaccttttaa atgcaatata
    11341 gacttaataa cttcttacaa agtctattat taataccgta gatattcatc ttaccctgtg
    11401 caccaaaagc aaaaacttat cgacgcgggt ttgaaactga tggcaaccgc aaaaatgcct
    11461 gatgcgctac gcttatcagg cctacgccat ctctgcaata tattgaattt gcgtgctttt
    11521 gtaggcagga taaggcgttc acgcagcatc cggcatgaat aaagcgcact caacaatctg
    11581 aaacccgccg gaacggttta ttacgtacat caggtgaaac tgaccgataa gccgctttct
    11641 tttgggtata gtgtcgtgga cagtcattca tctttctgcc cctccaaaag caaaaacccg
    11701 ccgaagcggg tttttacgta tatcaggtga aactgaccga taagccgctt tcttttgggt
    11761 atagcgtcgt ggacagtcat tcatctttct gcccctccaa aagcaaaaac ccgccgaagc
    11821 gggtttttac gtacatcagg tgaaactgac caataagccg ctttcttttg ggtatagcgt
    11881 cgtggacagt cattcatctt tctgcccctc caaaagcaaa aacccgccga agcgggtttt
    11941 tacgtaaaac aggtgaaact gaccgataag ccgggttctg tcgtggacag tcattcatct
    12001 aggccagcaa tcgctcactg gctcaagcag cctacccggg ttcagtacgg gccgtacctt
    12061 atgaacccct atttggcctt gctccgggtg gagtttaccg tgccacggac tgttaccagc
    12121 cgcgcggtgc gctcttaccg caccctttca cccttacctg atcccgcttg cacgggccat
    12181 cggcggtttg ctctctgttg cactggtcgt gggtttcccc cccaggcgtt acctggcacc
    12241 ctgccctatg gagcccggac tttcctcccc tccgcccgtc tcccccgaag aggacgacga
    12301 caaagcggcg actgtctggt cagcttcggc gcgcagtata gagggtttgc gcgcccttgt
    12361 caccccgtgt tgcgcattcc aatcgccagt gtcgcggcaa tattacgtga agcacggcag
    12421 atattgtcat aagccccgcg gaatgcttcg tccaacgtac ctatgctggt caatacgctg
    12481 aagaccgcat caatgccatg ctgatgtaca acgccaacat catcggtcag gctacccgca
    12541 cccgcaatgc caataaccgg tttatggtac ttcttcgcca cgtttgcgac gccaatcggt
    12601 accttgccgt gaatactctg gctgtcaata cgcccttcac cggtgatcac cagcgtacaa
    12661 tcgtgaatat gttcctccag attcagcgcc gtagtgacga tttcaatacc acttttcagt
    12721 tccgcaccaa gaaacgccat cagcgccgca cccataccgc ccgcagctcc tgcgccgggg
    12781 acatctttta catcaacatg cagcgctttt ttaatgacat cggcatagtg agagaggtta
    12841 ttgtccagct caacaatcat cgcttcactg gctccctttt gtgggccaaa gatgcgcgat
    12901 gcgccgttat cgcccaccag cggattggtg acatcacaag cgacgcgaat gacgcaatct
    12961 tttaagcgcg gatcgaggcc ggaaatatca atatcattca gagtattaag actaccgccg
    13021 ccaaaaccaa tttcattgcc gttggcgtcg cataatttcg cccccagcgc ctgtaccatg
    13081 cctgcgccgc catcatttgt agcgctaccg ccaatgccga taataatgtt tgtcgcgccg
    13141 ctctccagcg cctgcaggat taactcgcct gtgccgcgtg aagtggtcac gagtggatcg
    13201 cgtttttccg caggtaccag ctccagccca ctggccgccg ccatttcaat aaacgcggtt
    13261 ttgccatcgc cggagatccc ccaactggca ttcactttct cgcccagcgg ccctgtaacc
    13321 caggtgtgac gttcagaacc ctgggtggct gcaatcatcg cttccaccgt tccttcgcca
    13381 ccgtcggcaa ccggaataga aacgtactgt gcatcaggaa aaatttcccg aaatcctttt
    13441 tctatcgcct gcgcaacctc gctggcagat aaactttctt tataagagtc tggggcgatt
    13501 acgattttca tacctatgcc tgttaccaca tgacgccgga gggcgtttct cttattcggc
    13561 ctggattcca ggcccggatt gcaatacgcc atccgggcac gacgtcatta acgagtaact
    13621 tcgactttcg ccagtttttc gtagtagcac gccagggcgc tatgatccgc cgttcctaaa
    13681 ccatctgctc gcagtgcctg catcatctcc ataaccgcag ctgtgagcgg cagttgcgcg
    13741 ccgacgccgt gggaagtatc cagcgcattt gccagatcct taatatgcag atcaatacgg
    13801 aagcccggct tgaagttgcg gtccatcacc atcggcgctt tggcatccag cacggtactg
    13861 cccgccagtc caccgcgaat tgcctgatac accaggtccg ggttaacgcc cgctttagtt
    13921 gccagcgtta acgcttctga catcgcggca atattcagcg ccacaatgac ctgattcgcc
    13981 agtttggtga cgttacctgc accaattgcc ccggtatgca ccacggaacc cgccattgct
    14041 ttcatcaaat catagtattt gtcgaaaata gccttgtcgc cgcccaccat cactgacagc
    14101 gtaccgtcga tggctttcgg ttcaccgccg ctcaccggag catccagcat atcaatgcct
    14161 ttcgctttca gcgcttcgct gatttcacgg cttgccagcg gtgcgataga actcatatcg
    14221 atcaataccg tacctggctt cgcgccttca ataatgccat tctcacccag cgccacctct
    14281 ttcacatgag gggagtttgg cagcatggtt atgatgacgt cgcactgttc agcgatcgct
    14341 ttagccgtag aggctgtttc tgcacctgca gcaatcacgt cagcaatagc ttctgggtta
    14401 cggtcagcaa ccaccagcga gtaacctgct ttcagaaggt ttttactcat tggtttaccc
    14461 ataatcccta ggccaataaa accaactttc atagtcatat caatcatctc tcttgttgcg
    14521 gtggtggtta ttttttaaag gtatcagcca gtttatgagt ggcagagcgg aagacgccga
    14581 gatcgctgcc gacagccaca aacgtcgcgc cccattccag ataacgacgc gcatcggctt
    14641 cgaccggcgc gaggataccg ctgggtttgc cgtgcgcgct ggcacggtta aaaatgtgct
    14701 gaattgcttt ttgtacatcc gggtgtgatg cattgccgag atggcctaat gccgcggcca
    14761 gatcgctggg gccgacgaag atgccgtcta cgccttcggt agcggcaatg gcatcgacgt
    14821 tatctacgcc ctgctgactt tctatctgga ccagaatagt gatgttcttg ttcgactgag
    14881 cgaaataatc cgccacggtg ccaaacatat tggcgcggtg agaaacggag acgccgcgaa
    14941 tgccttccgg tgggtaacgg gttgatgcca ccgcctgctc tgcttcctct tttgtttcta
    15001 caaaaggaat caggaagtta tagaaaccga tatccagaag acgcttaata attaccggct
    15061 cgttggtcgg cactcgcact actggcgcgc tggcgctgcc tttcaaggcc attaactgcg
    15121 gaataaacgt ggagatatcg tttggcgcat gttcgccatc cagcaccagc cagtcaaacc
    15181 cagccaaacc aagaacttca gtgctaatcg ggttagagag tgctgaccag caaccaattt
    15241 gtacctgttt cgcagccagt gcggctttga atttattcgg gaaaacatcg ttattcatcg
    15301 cttatacctt tgcttatttc tgcaattcca tacgtttaat gtcgccaact acgaagaggt
    15361 agcagaccat cgccatcagc gctgaacatc ccacgaaaac cagtgctgca ttgaaggagt
    15421 gcagttcact taccaggtag ccaatcacca gtggagtgac aatggaggca acattgccaa
    15481 agacgttaaa gacgccgcca cagaggccaa caatctcttt cggcgcggtg tcagaaatca
    15541 ccggccagcc cagagcacca aatcctttgc caaaaaaagc cagcgccatc agcatgacca
    15601 ccagcgtggt gttgttggtg tagttacata agatgatggt ggaagccagc aacattccca
    15661 gcacaatcgg tagcttacgt gccagggtca gggataaacc gcgtttgatc agataatccg
    15721 agaagacacc tcccagcacg ccgcccgcaa aaccacacag tgccggaatc gaggcgacca
    15781 gacccacttt cagaatcgac atgccttttt cctgcaccag ataaatcggg aaccaggtga
    15841 ggaagaacca ggtgatggtg ttgataaaat attgtccgaa aaatacgccc aacatcatgc
    15901 ggttagagag caattgcttg atgtaatgca gtttgggacc gcttgctgcc gcgctgcccg
    15961 gctttttgtg gtccatatcg accaccgcgc cattttcaga gataaacttc agctcttcct
    16021 cagacatacg tgggtgatct gtcgggttat gaatcaactt gatccacagc gccgtcagca
    16081 caaaaccaat cacccccata acggtaaaga cgtgctccca tccccaggcg aaagtcagcc
    16141 agccaagcag aggcgaaaag agcgccagcg agaaatattg tgccgagtta aagatggcgg
    16201 aggcagtacc acgttctttc gtcgggaacc aggcggcgac aatacgggcg ttcgccggga
    16261 atgatggagc ttccgagaat cccagcataa agcgcataaa gaacatggag atccctgccc
    16321 aggccagcgg gaacatatca acaaagcctt gcaggaaggt gaacagcgac cagaaaaaga
    16381 ggctgtaggt gtaaactttt ttcgagccaa acttatcaag caaccagccg ccggggattt
    16441 gcatcagcaa gtaggcccag ccaaaagcgg agaagatgta acccatcgaa accgcactta
    16501 actgcaactc ttttgccact tcggtaccag caatagacag cgttgcacga tcggcgtagt
    16561 taacggcggt aacaataaaa ataatcagta atattaaata gcgggtatgc acacctttcc
    16621 ttttttcgtc aacggtgtcc agaatcattt tatttacctc gggtacttat gctgattttt
    16681 attattatgg ggaaggtgtt atttataagt ttcatttatg ccgtaacgac aatgaactcg
    16741 ggaattagta taagcagcgc gagaataata atcattgtgc aaatgctaat ttaattaata
    16801 ctttttaaat attattttga gcatatgcac ataaggttgc gcgctaaagc acagatttgc
    16861 gctttacctt gccgggcggc actgcaatcc ctgaaatgat tgacattgat cacatttctg
    16921 cgtttaaact cctgacattc ttatttcacc caatgaagtc atttattttt aaatgagacc
    16981 aggtcctcat tttaataacc cctggctgga gaatattgca caatggccaa catcgaaatc
    17041 agacaagaaa cgccaactgc gttttatata aaagttcacg acacagataa tgtggcaatt
    17101 attgttaatg ataatggcct gaaagcagga acgcgttttc cggatgggct ggaattaatt
    17161 gaacatattc cccaggggca taaagtcgca ttgctggaca ttccggctaa tggtgaaatt
    17221 attcgttatg gcgaagtgat tggttacgcc gtgcgtgcaa tcccacgcgg aagctggatc
    17281 gacgaatcaa tggttgtact accggaagcg ccgccgttac acacgctgcc actggcaacc
    17341 aaagtcccgg aacccttacc gccgctggaa ggatacacct ttgagggcta tcgcaatgcc
    17401 gatggcagcg tgggcaccaa aaacctgctc ggcatcacca ccagcgtcca ctgtgtggca
    17461 ggcgtggtgg actatgtagt aaaaatcatt gaacgcgatc tgctaccgaa atacccgaac
    17521 gtcgatggcg tggtggggct gaatcatttg tacggttgtg gcgtggcgat taacgcaccg
    17581 gcggcagttg tacctattcg taccattcac aatatttcgc tgaaccctaa ctttggcggc
    17641 gaagtaatgg tgattggcct gggttgtgaa aagttgcagc ctgagcgcct gctgactgga
    17701 acggatgatg tgcaagctat tccagtagaa agcgccagca ttgtcagttt gcaggatgaa
    17761 aagcatgtcg gttttcagtc catggtcgag gatattttgc aggtcgccga acgccatcta
    17821 caaaaactga atcaacgtca gcgagaaacc tgtccggctt cagaactggt tgtcggcatg
    17881 cagtgcggtg gcagcgatgc gttttctggc gtaacggcaa acccggcggt tggctatgcg
    17941 tctgatctac tggtgcgctg cggcgcaacg gtgatgtttt cagaagtcac ggaagtgcgt
    18001 gacgcgatcc atctgctgac accacgcgca gtgaacgagg aggtcggcaa acggctgctg
    18061 gaggagatgg agtggtacga taactatctc aatatgggaa aaaccgaccg cagcgccaac
    18121 ccttcgccgg gcaacaagaa aggcggtctg gcaaacgtgg tagagaaagc gctcggctcc
    18181 attgctaaat cgggcaaaag tgcaattgtt gaagtgctgt cgcccggtca acgcccgact
    18241 aaacgcggat taatttacgc cgcgacgcca gccagcgatt ttgtctgtgg cacgcaacag
    18301 gtggcttcgg gtatcaccgt gcaagtgttt acgaccggtc gtggtacgcc gtacggcctg
    18361 atggcggtac ccgtcattaa aatggcaacc cgcaccgagc tggcgaaccg ctggtttgat
    18421 ttaatggata ttaatgcggg caccatcgct accggcgaag aaactattga agaggtgggc
    18481 tggaagttgt tccactttat tctcgacgtc gccagcggga agaagaaaac cttctcggat
    18541 caatggggac tgcataacca gctggcggtg tttaacccag caccggtgac ctgataattc
    18601 aattcgtatt atgcccgata gcacacgttt atcgggcata ttgagtcaca gcgaagacgc
    18661 gtgatccccg accagaatca cttcaacccc agcctttcgc agtccttcca gactatccgc
    18721 aggaatgcct tcatcaacaa tgatcatgtc gatacgttga gtatcaatga tcttatgtaa
    18781 actggaacga ttgaatttac tggaatcggt gaccacgatg atccgttccg caacttcgca
    18841 catccgacgg tttaaacggg cttcatcttc attatgtgtg ctgacgccgc gctccagatc
    18901 gatcgcatct acaccaagaa acagcatatc gaagtggtaa ttttgcagcg attgctcagc
    18961 ctgatcgccg taaaaagatt gcgactgacg gcgcaaatgc ccgccggtca tcagcagctc
    19021 aacgccttcc gcttccagca acgcattagc cacgttcata ccgttggtca tcgcaattac
    19081 gtcagtgtac ttgcgcatca gacgagcaat ctcaaaagtg gtggtcccgg aatcgaggat
    19141 cacccgatga cctggctgaa tcaactcaac ggcagctttc gcaacgctgc gtttcatcgc
    19201 ggtgttcagt gcgcttttat cttccactga tggctcgact gacggcgtcg tgctatcgca
    19261 gatcaacgcg ccaccatagg cacgcacagc gatcccctgc ttttccagaa aagccagatc
    19321 attgcggatc gtcacagtag atacgccata caatgccgac agatcgttaa cctgcacact
    19381 cccttgctgt cgcagacgct gaatgatctg ttctcgtcgc tcgctggtgc ctgtcactcg
    19441 cttctcacct gaagcgtcgg tattactcat agtaagtcct ttcgtaaaac tttcgtttca
    19501 tttcgttttg cctattaacg cctttctatt aagcaaatgc aagcccatct tgcccattgg
    19561 cgcaagctac tctcgtttca gtgactttca ttatgtttct tttgtgaatc agatcagaaa
    19621 actattatat ttcgttttat ttttatctca ccatgatgca gtattaattg aaacaaaacg
    19681 aaagattaat atcgcagtaa tctgaactgg agaggaaagt gaaacatctg acagaaatgg
    19741 tgagacagca caaagcgggc aaaacaaatg caatttatgc cgtttgttcc gcacatccgc
    19801 tggtgctgga ggctgcaatc cgctacgcca gtgcaaacca aacgccgtta ctgattgaag
    19861 caacctccaa tcaggtagac cagttcggcg gttataccgg aatgacgccc gccgattttc
    19921 gcggctttgt ttgtcagctc gccgactcgt tgaatttccc gcaggatgcg ctgattctgg
    19981 gtggtgacca tctggggcca aaccgctggc aaaacctgcc agccgctcag gcaatggcca
    20041 atgccgatga tttgattaaa agctacgttg cggcaggatt caaaaaaatc caccttgatt
    20101 gcagcatgtc ctgtcaggac gatccaattc ccttaactga tgacatcgtg gctgaacgcg
    20161 ccgcccgtct ggcgaaagtg gcggaagaaa cctgtcgcga acactttggc gaagccgatc
    20221 tggagtatgt cattggtacc gaagtgccgg tacctggcgg cgcgcatgaa accttaagcg
    20281 agctggcggt caccacgccg gatgccgccc gcgccacgct ggaagcccat cgtcacgctt
    20341 ttgaaaagca aggtttgaat gccatctggc cacgcatcat tgccctggtg gttcaacccg
    20401 gcgtcgaatt cgatcacacc aacgttattg attatcagcc cgccaaagcg agcgccttaa
    20461 gccagatggt cgaaaactac gaaacgctga ttttcgaagc gcactctacc gattatcaat
    20521 tgttttaagc cggattgatg aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg
    20581 atttgcgggg gcaaatctgg tgctggtagc caacgatgag gttgccgaag atccggtaca
    20641 acaaaacctg atggaaatgg tactggcaga agggatcgcc gtacgtttct ggacgctgca
    20701 aaaagttatc gacaacattc atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa
    20761 aacacccgcc gatttcctga cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt
    20821 tggcaatatg cactacgcca atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc
    20881 gggcgatatc gcagcattta acgacctgaa agccgctggg gtggaatgct tcgttcaggg
    20941 cgtcccgaca gagcctgctg tggacctctt taaattactt tgagggattc atcatggaaa
    21001 tcagcctgtt gcaggcattt gcgttgggca ttatcgcctt tatcgctggc ctagatatgt
    21061 ttaacggctt aacccatatg caccgcccgg tggtcctcgg cccgttggtc gggctggtac
    21121 ttggcgatct gcataccgga attttaaccg gcggtacgct ggaactagtg tggatggggc
    21181 tggccccgct ggcgggcgca cagccgccta acgtgattat cggtactatc gtcggcacgg
    21241 cgtttgccat tactactggc gtgaaacccg atgtcgcggt cggtgtcgcc gtacctttcg
    21301 ctgtcgcagt acagatgggg attaccttcc tgttctcggt gatgtctggc gtgatgtctc
    21361 gctgcgaccg gatggcggag aacgccgata ctcgcggcat tgaacgcgtg aactatctgg
    21421 cactgctggc actcggcact ttctattttc tctgcgcttt cctgcctatc tacttcggca
    21481 cggaacatgc aaaaaccatc attgatgtcc tgccgcaacg attaatcgac ggcctcggcg
    21541 tcgcgggcgg catcatgcca gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa
    21601 acgtctacat accctacttc atcctgggct ttgttgccgc agcctggctc aagttaccgg
    21661 tgctggctat tgctgccgcc gcgctggcga tggcgctgat cgatctgctg cgtaaatctc
    21721 ctgaaccgac tcaacctgcg gcacagaaag aggaatttga agatggcatc taatcaaacc
    21781 accctgccga acgtcagcga aaatgaagaa acactgctga ctggcgtcaa tgaaaacgtg
    21841 tatgaagatc ggagcattgc cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg
    21901 cgttccatgc tgttacaagc gtcttttaac tacgaacgta tgcaggcttc tggctggttg
    21961 tacggtctgc tgcctgctct gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc
    22021 atgaaggggc atatgggttt cttcaatacc catccgtttc tggtgacgtt tgttatcggc
    22081 attatccttg cgatggagcg ttctaagcag gacgttaaca gtattcagag caccaaaatt
    22141 gccgtcggtg cgccgcttgg cgggattggc gatgcgatgt tctggctaac gctactgccg
    22201 atttgtggcg ggataggcgc aagcctcgcg ctacaaggct ctattcttgg cgcagtcgtc
    22261 tttattgtgc tgttcaacgt ggtacacttg gggctgcgtt ttggtctggc gcattatgct
    22321 taccgcatgg gcgtggcggc gattcccctt attaaagcga ataccaaaaa agtcggccat
    22381 gctgcgtcaa tcgttggaat gacggtaatc ggcgcactgg tggcaaccta tgttcgttta
    22441 agcaccacgc tggaaatcac ggcgggcgac gcagtggtta agttacaggc tgatgttatc
    22501 gacaaactga tgcctgcctt cttaccgctg gtctacaccc tgaccatgtt ctggctggta
    22561 cgccgcggct agagtccgct gcgcctgatt gcggttaccg tggttctagg gatcgtcggt
    22621 aaattctgcc atttccttta aatacaatga ggtattagat gttaagtatt attttgacag
    22681 ggcatggcgg gtttgccagt ggcatggaaa aagcaatgaa gcaaatcctc ggcgatcaat
    22741 cgcagtttat tgccatcgat tttcccgaaa cctcaagcgc cgcgctgctt acatcgcagc
    22801 ttgaagaggc catcgcccaa ctggattgtg aagacggcat tgtttttctg acggatttgc
    22861 tgggcggcac gccgtttcgc gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag
    22921 tgattaccgg caccaatttg caactgctac tggagatggt gctggagcgc gaagggttaa
    22981 gcggtgaaga attccgtgta caggcgctgg agtgtggaca ccgagggctg accagtctgg
    23041 tggacgagtt aggccgctgc catgaagaat atccggtcga ggaaggaata tgacacacgt
    23101 tctgcgcgcc agaaggctgc tgactgaaga gggatggttc gatgaccatc agttgcgtgt
    23161 tgctgacggt gtcatcgcag caatcgaacc gattccagtg ggcgtgactg aacgcgatgc
    23221 ggaactgctc tgccccgctt acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt
    23281 aatggatgac gcgccggatg tactcgacaa gctggcaatg cacaaggcac gcgaaggtgt
    23341 cggcagttgg ttaccgacca ccgtaaccgc gccgcttaat accattcatg cggcgctgaa
    23401 acgtattgct caacgttgcc aacgcggcgg acctggtgcg caagtgctgg ggagttatct
    23461 cgaaggaccg tacttcacgc cgcagaataa aggcgcgcat
//
LOCUS       DAJTNM010000056        23223 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTNM010000056 DAJTNM010000000
VERSION     DAJTNM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.056"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="OO172_002272"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="OO172_002272"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5512444.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="OO172_002273"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="OO172_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5512445.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OO172_002274"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OO172_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5512446.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OO172_002275"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OO172_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5512447.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OO172_002276"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OO172_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5512448.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="OO172_002277"
     CDS             5281..5739
                     /locus_tag="OO172_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5512449.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="OO172_002278"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="OO172_002278"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5512450.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="OO172_002279"
     tRNA            6791..6875
                     /locus_tag="OO172_002279"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="OO172_002280"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="OO172_002280"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OO172_002281"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OO172_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5512451.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OO172_002282"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OO172_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5512452.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OO172_002283"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OO172_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5512453.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="OO172_002284"
     CDS             complement(10156..13899)
                     /locus_tag="OO172_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5512454.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OO172_002285"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OO172_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5512455.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="OO172_002286"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="OO172_002286"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5512456.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="OO172_002287"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="OO172_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5512457.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVIFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OO172_002288"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OO172_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5512458.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="OO172_002289"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="OO172_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5512459.1"
                     /translation="MKKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OO172_002290"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OO172_002290"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5512460.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OO172_002291"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OO172_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5512461.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="OO172_002292"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="OO172_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5512462.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5823 a         6059 c         5588 g         5753 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgtt tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taatgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgcc tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 atagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaagc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacgaagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacggtaa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgaccggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattg
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgctga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat gaccggattt gcgcatactg aatggttggg attgaccgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggtctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggac gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcgc aacgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct ccccgataag ggagcaggct agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact ttcaaaagcc ccggaattct
     6901 caaacgaatc tgcaatcaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttgaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgtttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca gcaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacatac gacgtttcag caaaagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcaacatacc gaagaagtga ccgacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actggacctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 agccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaaca gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagcgcacg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaattccgtt gctgcccagc tcgcccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccatcgc cattttataa accggcgact
     9181 gctgtgcagc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacgtcca gctgacgctg gtagagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacggac
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt atcaaactca ccgcccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgataat
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcaaagattt
     9901 tacccagcag catggctcgg ttacccatgc gcggttgcag tttgccattg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tggcccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagtgc accgttgctg ttgaagactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttt gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccgttaccg attttctcca tcagcgggcc gatagaggta aagcgttcgt aagttgccgg
    11221 ataatcgcgc tctacgacca taatgtgcgg tgcagttttg cctgggatca ggtcgcattc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgctttta gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaagaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagttacag cataacatgc tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcaaatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taacactaat
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcggcatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaagtaat cactacgcag
    14341 gcgatataga aaatcaaaaa tactttcatt gcacctactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatcg cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgcc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gatgaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa accgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtta tgacagctcc agtagccctt
    15661 tcggaggcag atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgga gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt agaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccggctat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatatatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aaggggttta
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gcgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttga ctcgagcagc agaacgagac ggacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgcg ctgatgcgtg cacaaaaccg
    16381 agaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgtattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagc catcgcgatt ttacccaacg
    16621 cgcaaacatc agcgggcgca acgaaatggc gatgcttgga actgcgttga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc ggtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaactgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actggtggat accctggttg aacaacttac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggaag cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgtct ggtgccttca catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaggtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tgatattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggctatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc acagacaatc gccagtgatt gcttgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgca tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tgtcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg tacaccaaag cggtaattaa gattcagccc gaggttattt
    18961 tgattttcgc cactttcccc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc aaagttaaac
    19081 aaatcaacac gatcaccaaa atactgttct aggctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgagc tgtcaggtcg tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgataa tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtcctg caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccattatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg attttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgctacaa gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aatgtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgaaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctctgattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgttta
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccttaaca
    20461 caccatccgg ataattctcc gccagtagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcat
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcatgatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcatcagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccagaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagtagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtttt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccaggcc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc ctgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aacgccagct cgctggagac attgctcgac agcatgaatg cccctgttgc
    22561 ctttactggc ttcctggtgg cactgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg ttcagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aacaatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 gcagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggagagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaa
//
LOCUS       DAJTNM010000057        23173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTNM010000057 DAJTNM010000000
VERSION     DAJTNM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.057"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..315)
                     /locus_tag="OO172_002293"
     CDS             complement(<1..315)
                     /locus_tag="OO172_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5512463.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASK"
     gene            complement(384..1073)
                     /gene="mutH"
                     /locus_tag="OO172_002294"
     CDS             complement(384..1073)
                     /gene="mutH"
                     /locus_tag="OO172_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5512464.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1758..2288
                     /gene="rppH"
                     /locus_tag="OO172_002295"
     CDS             1758..2288
                     /gene="rppH"
                     /locus_tag="OO172_002295"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5512465.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            2301..4547
                     /gene="ptsP"
                     /locus_tag="OO172_002296"
     CDS             2301..4547
                     /gene="ptsP"
                     /locus_tag="OO172_002296"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5512466.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            4698..5573
                     /gene="lgt"
                     /locus_tag="OO172_002297"
     CDS             4698..5573
                     /gene="lgt"
                     /locus_tag="OO172_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5512467.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINSELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            5580..6374
                     /gene="thyA"
                     /locus_tag="OO172_002298"
     CDS             5580..6374
                     /gene="thyA"
                     /locus_tag="OO172_002298"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5512468.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            6558..7028
                     /locus_tag="OO172_002299"
                     /pseudo
     CDS             6558..7028
                     /locus_tag="OO172_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            7019..7582
                     /locus_tag="OO172_002300"
     CDS             7019..7582
                     /locus_tag="OO172_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5512469.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            7579..7986
                     /locus_tag="OO172_002301"
     CDS             7579..7986
                     /locus_tag="OO172_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5512470.1"
                     /translation="MNREKGVSSQALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            7971..8294
                     /locus_tag="OO172_002302"
     CDS             7971..8294
                     /locus_tag="OO172_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5512471.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPLANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            8307..11675
                     /gene="recC"
                     /locus_tag="OO172_002303"
     CDS             8307..11675
                     /gene="recC"
                     /locus_tag="OO172_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5512472.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            11851..14739
                     /gene="ptrA"
                     /locus_tag="OO172_002304"
     CDS             11851..14739
                     /gene="ptrA"
                     /locus_tag="OO172_002304"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5512473.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRMADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            14732..18274
                     /gene="recB"
                     /locus_tag="OO172_002305"
     CDS             14732..18274
                     /gene="recB"
                     /locus_tag="OO172_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5512474.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            18274..20100
                     /gene="recD"
                     /locus_tag="OO172_002306"
     CDS             18274..20100
                     /gene="recD"
                     /locus_tag="OO172_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5512475.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(20162..21493)
                     /gene="argA"
                     /locus_tag="OO172_002307"
     CDS             complement(20162..21493)
                     /gene="argA"
                     /locus_tag="OO172_002307"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5512476.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            21725..22978
                     /gene="amiC"
                     /locus_tag="OO172_002308"
     CDS             21725..22978
                     /gene="amiC"
                     /locus_tag="OO172_002308"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5512477.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(23053..23129)
                     /locus_tag="OO172_002309"
     tRNA            complement(23053..23129)
                     /locus_tag="OO172_002309"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23093..23095),aa:Met,seq:cat)
BASE COUNT         5469 a         5788 c         6378 g         5538 t
ORIGIN      
        1 cttgctggct ttccagataa ggaacaagcc acccagaagc agaatcaaat cacgggcaga
       61 aatttcctga ctgaatattg taaaaagcgg atttgtcagg cgcgtaaccc aagagattga
      121 tgccagcaga gccagacgca taaccatggc tcccgccaac cccagacggc gcgcatgagc
      181 acgttgtgct gtgggaagct ttgccaccac cagagaaagg aaaataatat tgtcgatccc
      241 aagaacgatc tccagcagcg tcagcgtacc gagcgcaagc caggcgttag gatcggttat
      301 ccatgcaaat aacatctaaa aagccctgcc aaaaaaatga agcggtaatt atatgcccgg
      361 aaagcggcag gtcaaagcga tggctactgg atcagaaaat gacgggccag tagtgcactg
      421 gtgaaattct tcttcaaata aaagccgcgt ggcagcgtca gaatccgttc gccccgggca
      481 ccaatggctt cggtaagcgc tttcgcattc gctgctttcg gtcttatctg taaatattcc
      541 ccgtgacgag cggtgatccg ctcaacctga ccgagaacaa tcatatccat taattcttcc
      601 cagtcttcgc gtagctgtcg gtcttcctct tcattcgggc tccacagcaa cggtgatcct
      661 acgcgacgct gcgccagcgg gatgctgcgc tcgccttcaa ccggtatcca cagtacgcgt
      721 ttgagcttgt ggcgcacatg gctggtttcc caggtcaccc cgctattacc cgttaacggg
      781 gcaacacaaa cgaatgttgt ttccagcgga cgaccaagac tatccacagg gatagtttta
      841 agttccacgc ccagagcagc aaaatcttgc tcaggtttac tccctgcgct ggcacctagc
      901 cagatctcca gtaacacgcc aatccagccc ttatcgcgtt ttaaattctc tggcgtaacc
      961 agcccggcaa gtgccgccag ttctcccaat gtataaccag aaagttgctg tgcttgcgct
     1021 aacaactgtt cttcagtttc gggaggagag agcagtgggc gaggttggga catgtcatga
     1081 tgccttggtt aaaaaacaaa cagcaattat gtgccgaata ttcgcagtgt aactttttcc
     1141 acatgtgttg ttcaattctc tgatttatat tgctttttag gctgccggta atcgagttgc
     1201 gcaacactgc aataggtttt ccaaatctgg tcactgaaaa taaacaggat cttacaccat
     1261 gttatccaca gaaagctggg ataactgtga aaaacctcca ctactgtttt catttacagc
     1321 cttgacgtgc gacgaaactc cagtttttag tggttcagtg cttaacttag tggcacattc
     1381 tgtggataaa tacggcattg gttgatcttt cgccaacccg taaatgagag gtgtgacctg
     1441 ggtcacgaat tactcttttt cattactcaa attacatgta atgcattgac taataatgaa
     1501 tattttaaaa tgtctatatc gggcttattc agaatgctcc gggttttccc gtagtaattc
     1561 cgtagttctt cacaactcta tccacagaaa aggtgaataa aaacggctat ccaccccttc
     1621 ctctgtttat aactctgcac ataactgtga gttattcaat tgttattagt gtgtaatgcc
     1681 ctgcagagag tggtttaccg tcttcctgga gtatgaaaca atcattcgta tataaagctt
     1741 tattttgagg tagtccggtg attgatgacg atggctaccg cccaaacgta ggtatcgtga
     1801 tttgtaatcg ccaggggcag gtaatgtggg cccggcgatt tggtcagcac tcctggcaat
     1861 ttccgcaagg cggaatcaac cccggagaat ccgcagagca ggcgatgtac cgtgaattgt
     1921 ttgaagaagt aggattaagc cgcaaagacg ttcgaatcct tgcttcaacg cgtaactggt
     1981 tgcgctacaa attaccgaaa cgtttggtgc gttgggacac gaagccggtt tgtatcggcc
     2041 aaaaacaaaa atggtttctc ttgcagctgg taagcggcga tgcagaaatc aatatgcaaa
     2101 ccagcagtac accagagttt gacggctggc gatgggtaag ttactggtat ccggtcagac
     2161 aggtggtgtc atttaaacgt gatgtctacc gtagggtaat gaaagagttc gcgagtgtgg
     2221 tgatgtcact gcaggaaaat acgccaaaac cacaaaacgc atctgcttat cgacgtaaaa
     2281 gaggttaagt cacgccaatt atgctcactc gcctgcgcga aatagtcgaa aaggtagcca
     2341 gcgcaccacg cctgaatgag gcgttaaata ttctggttac cgacatctgt cttgcgatgg
     2401 ataccgaggt ctgttcggtc tacctggccg atcatgatcg acgttgttac tacctgatgg
     2461 cgacccgggg gctgaaaaaa ccacgcggtc gcactgtaac gctcgcgttt gatgaaggga
     2521 tcgtcggcct ggttggcagg ctggcggaac cgataaacct tgcagatgcg caaaagcacc
     2581 ccagcttcaa atacatcccc tccgtaaaag aagaacgttt ccgcgcgttt ttaggcgtac
     2641 caattattca acgtcgccag ttgcttggtg tactggtggt acagcaacga gagttgcgcc
     2701 agtatgacga aagtgaagaa tccttcctgg tgacgcttgc cacccagatg gcagctattc
     2761 tttctcagtc gcagttgact gccctgtttg ggcaatatcg ccagacgcga atccgcgcat
     2821 taccggcagc acctggtgtg gcgattgccg aaggctggca ggatgccacg ttacctttaa
     2881 tggaacaggt gtatcaggca tcaacgctgg atccggctct ggaacgcgaa cgactgaccg
     2941 gggcgctgga agaagcggca aacgagtttc gccgctacag caaacgcttt gccgccggag
     3001 cacaaaaaga aacggcggct attttcgatc tttactcgca cctgctttcg gacacccggc
     3061 tgcgtcgcga attgtttgcc gaggttgata aaggctcggt ggcagagtgg gcggtaaaaa
     3121 cggtcattga aaaatttgcc gaacagtttg ccgcgctaag cgataactat ctcaaagagc
     3181 gggctggcga tttacgtgcg ctgggtcagc gattgctgtt tcatcttgat gacgctaatc
     3241 aagggccgaa cgcctggccg gaacgtttca ttctggtggc agatgaactg tcagcgacaa
     3301 ctcttgctga gctgccccag gatcgcttag tcggtgttgt cgtgcgagat ggcgcagcca
     3361 actcccatgc tgcgatcatg gtacgtgcgc tggggatccc taccgtgatg ggcgcggata
     3421 ttcagccttc ggtgctgcat cgtaggacgc tgatcgttga tggctatcgc ggtgaattgc
     3481 tggtcgatcc ggagccggta ctgctgcaag aatatcagcg gctaattagt gaagagattg
     3541 agcttagccg tctggcggaa gatgacgtca atttacccgc gcagttaaaa agcggtgagc
     3601 gtataaaagt catgctcaat gctggtttaa gcccggaaca tgaagaaaaa ctgggcagcc
     3661 gtattgatgg catcggtctt tatcgcactg aaatcccatt catgctgcaa agtggtttcc
     3721 cgtcggaaga agaacaggtg gcgcagtatc aggggatgct gcaaatgttc aatgataaac
     3781 ccgtcacctt gcgtacgctg gatgtcggag cagataagca gctgccttac atgccgatca
     3841 gcgaagagaa tccatgcctg ggttggcgtg gtattcgcat tacgctcgat cagccggaga
     3901 tcttcttgat ccaggtgcgg gcgatgctgc gtgctaatgc cgctacgggc aacctgaata
     3961 ttctgttgcc gatggtcacc agcctcgatg aagttgacga agcacgccgc ctgattgaac
     4021 gtgccggacg tgaagtcgag gagatgatcg gttacgaaat tcccaaacca cgtatcggca
     4081 tcatgctgga agtgccgtca atggtattta tgctgccgca tctggcaaag cgggtcgatt
     4141 tcatctctgt tggcaccaac gatctgactc aatacattct ggccgttgat cgcaacaata
     4201 cccgggtggc taacatttac gacagtcttc atcctgcaat gttacgagct ctggcgatga
     4261 tcgcccggga agcggaaata catggtatcg atctccgttt gtgcggtgaa atggcgggcg
     4321 atcccatgtg tgtggcaatc ctcattgggc ttgggtatcg ccatctgtct atgaacggac
     4381 gttctgtagc gcgggtaaaa tacctgctgc ggcgcattga ttttgccgaa gcagaaaatc
     4441 ttgcgcagcg tagtctggaa gcgcaactgg cgaccgaagt tcgtcatcag gttgcagcct
     4501 ttatggagcg tcgcggcatg ggcgggctga ttcgcggagg gttatagcgc ggatcatata
     4561 catatctttt aacggtatcc ggcaaccagc caggtcccct tgtgctatta ttcgcacctt
     4621 tggagcgcct gaaacctgcg gcgcgcattt caatcgctgt tctctttcag cgaaataaca
     4681 agaacttgtg gtgacagatg accagtagct atctgcattt tccggagttt gatccggtca
     4741 ttttctcaat aggacccgtg gcgcttcact ggtacggcct gatgtatctg gtgggtttca
     4801 tttttgcaat gtggctggca acacgtcggg cgaatcgtcc gggcagtggc tggaccaaaa
     4861 atgaagttga aaacttactc tatgcgggct tcctcggcgt cttcctcggg ggacgtattg
     4921 gttatgttct gttctacaat ttcccgcagt ttatggccga tccgctgtat ctgttccgtg
     4981 tctgggacgg cggcatgtct ttccacggcg gcctgattgg cgttatcgtg gtgatgatta
     5041 tcttcgcccg ccgtactaaa cgttccttct tccaggtctc tgattttatc gcaccactca
     5101 ttccgtttgg tcttggtgcc gggcgtctgg gcaactttat taacagtgaa ttgtggggcc
     5161 gcgttgaccc gaacttcccg tttgccatgc tgttcccagg ctcccgtaca gaagatattt
     5221 tgctgctgca aaccaacccg cagtggcaat ccattttcga cacttacggt gtgctgccgc
     5281 gccacccatc acagctttac gaactgctgc tggaaggtgt ggtgctgttt attatcctca
     5341 acctgtatat tcgtaaaccg cgcccaatgg gagctgtctc aggcttgttc ctgattggct
     5401 acggcgcgtt tcgcatcatt gttgagtttt tccgccagcc cgacgcgcag tttaccggtg
     5461 cctgggtgca gtacatcagc atggggcaaa ttctttccat cccgatgatt gtcgcgggtg
     5521 tgatcatgat ggtctgggca tatcgtcgca gcccacagca acacgtttcc tgaggaacca
     5581 tgaaacagta tttagaactg atgcaaaaag tgctcgacga aggcacacag aaaaacgacc
     5641 gtaccggaac cggaacgctt tccatttttg gtcatcagat gcgttttaac ctgcaggatg
     5701 gattcccgct ggtgacaacc aaacgttgcc acctgcgttc catcatccac gaactgctgt
     5761 ggttcctgca gggcgatact aacattgctt atctacacga aaacaatgtc accatctggg
     5821 acgaatgggc cgatgaaaat ggcgacctcg ggccagtgta tggtaaacag tggcgcgcct
     5881 ggccaacgcc agatggtcgt catattgacc agattactac ggtactgaac cagctgaaaa
     5941 acgacccgga ttcgcgccgc attattgttt cagcgtggaa cgtaggcgaa ctggataaaa
     6001 tggcgctggc accgtgccat gcattcttcc agttctatgt ggcagacggc aaactctctt
     6061 gccagcttta tcagcgctcc tgtgacgtct tcctcggcct gccgttcaac attgccagct
     6121 acgcgttact ggtgcatatg atggcgcagc agtgcgatct ggaagtgggt gattttgtct
     6181 ggaccggtgg cgatacgcac ctgtacagca accatatgga tcaaactcat ctgcaattaa
     6241 gccgcgaacc gcgtccgctg ccgaagttga ttatcaaacg taaacccgaa tccatcttcg
     6301 actaccgttt cgaagacttt gagattgaag gctacgatcc acatccgggc attaaagcgc
     6361 cggtggctat ctaattacga aacatactgc cagagccgac gccagtgtgc gtcggttttt
     6421 ttaccctccg ttaaattctt cgagacgcct tcccgaaatt ttgcaacgtc ctgcaacggc
     6481 gtaaatagtc cggaagatgc gccgaagaaa tagaaactgg ccctcgtctt tttttctcct
     6541 cgctccatac tgccggcatg aaaacacaac gtggttatac gctgattgaa acgctggtcg
     6601 cgatgctgat tttggtcatg ctaagcgcaa gtggactcta tggctggcaa tactggcagc
     6661 agtcgcaacg gctttggcaa accgccagct aggcgcggga ctatttgctc tatttacgtg
     6721 aagatgccaa ctggcataac cgcgaccaca gtatcagcgt tatcagggag gggacgttat
     6781 ggtgccttgt gagttccgct gctggggcca atacctgtca tggcagttca ccattggtct
     6841 ttgtgccacg ctggcccgaa gtcgaaatga gcgacctgac accttcgctt gctttctttg
     6901 gcctgcgcaa taccgcatgg gccgggcata ttcgcttcaa aaactcaacg ggcgagtggt
     6961 ggctggtggt ttcgccgtgg ggaagactcc ggctttatca gcaaggagaa acagaaggat
     7021 gcctgtaaaa gagcaaggtt tttctctgct ggaagtgttg attgctatgg cgatcagtag
     7081 cgtattgttg ctgggggctg cacgctttct gcctgcgtta cagcgtgaaa gtttaacgag
     7141 cactcgtaag ctggcactgg aagatgaaat ctggctgcgg gtatttaccg tcgcgaagca
     7201 tctccagagg gcgggttatt gtcatggcag ctgtaccggc gaagggctgg aaattgtcgg
     7261 acagggtgac tgtatcattg tgcagtggga tgcgaacagt aacggtatct gggatcgcga
     7321 accggtaaaa gagtccgacc agattggatt tcgtctgaag gagcatgtgc tggaaacgct
     7381 acgcggtgcg acatcctgtg aaggtaaggg ctgggataaa gtcactaatc cggatgccat
     7441 cattatcgac acttttcagg tcgtacgtca ggatgtcagc ggcttctcgc cggtgttgac
     7501 ggttaatatg cgtgctgcca gtaagtctga accgcaaacc gtggtggatg ccagctatag
     7561 cgtgacagga ttcaacctgt gaaccgcgaa aaaggtgttt cgtcacaggc tctggtcctg
     7621 atgctgctgg ttttgggtag cttgctattg caaggaatga gtcagcagga tcgcagtttt
     7681 gcctctcgcg tgagcatgga aagtcagtca ttgcgccgcc aggccatcgt tcagtcggcg
     7741 ctggcgtggg gaaaaatgca ctcctggcag acgcagccag cagttcagtg ctcgcagtac
     7801 gctggaaccg atgcccaggt ttgtttgcgt ttactggcag ataatgaagc cttattgatt
     7861 gccggttatg aaggcgtttc gttgtggcga acaggcgaag tcattgatgg aaaaattgtt
     7921 ttttcgccac gcggctggag cgatttttgt ccgctgaaag agggggcatt atgtcagctt
     7981 ccctgaagaa tcaacaaggc tttagcctgc cggaggtaat ggttgcgatg gtgctgatgg
     8041 tgatgattgt cactgcgtta tcgggtatcc agcgaacatt aatgaacagt ctcgccagca
     8101 gaaaccagta ccaacagctc tggcggcatg gttggcagca aacgcaactg cgcgcgattt
     8161 cgccacttgc caactggcag gtcaaccgaa tgcagacatc gcaggcggga tgtgtcagca
     8221 tcagcgttac gctagtttca cccgggggca gagaaggcga gatgacccgc ctgcattgcc
     8281 cgaatcgtca gtagtcagga gccgctatgt taagggtcta ccattccaat cgtctggacg
     8341 tgctggaagc gttgatggag tttattgtcg aacgcgaacg gctggacgat cctttcgaac
     8401 cagagatgat tctggtgcaa agtaccggta tggcgcagtg gctgcaaatg accctgtcgc
     8461 aaaagtttgg tattgcggca aacattgatt ttccgctgcc agcgagcttt atctgggata
     8521 tgttcgtccg ggtgttaccg gaaatcccca aagagagcgc ctttaacaaa cagagcatga
     8581 gctggaaact gatgactctg ctgccgcaat tgctggagcg cgaagacttt accctgttgc
     8641 ggcattatct gactgacgat agcgacaagc gaaaactgtt ccagctttcc tcaaaagcgg
     8701 cggacctgtt tgaccagtat ctggtctatc gtccggactg gctggcacag tgggaaacag
     8761 gacatttggt tgaagggctg ggagaagcac aggcctggca agccccgttg tggaaggcgc
     8821 tggtggaata tacccatgaa ctcggacaac cgcgctggca ccgcgcgaat ctctatcagc
     8881 gctttatcga aacgctggag tccgcgacga cctgcccgcc ggggttacct tcgcgcgtct
     8941 ttatatgcgg tatttccgcg ttaccgcctg tttatctcca ggcgctccag gcgctgggta
     9001 aacatattga aatccatctc ctgtttacca acccctgccg ttattactgg ggcgatatta
     9061 aagatcctgc ttatctggcg aaactgctga ctcgtcagcg tcgacacagt tttgaagatc
     9121 gcgaattgcc gctatttcgc gatagcgaaa atgccggaca gctctttaac agcgatggtg
     9181 aacaggatgt cggcaacccg ctgctggcct catgggggaa gcttgggcgc gactacattt
     9241 atctcctttc tgacctggag agcagccagg agctggacgc ttttgtcgat gtgacgccag
     9301 ataacctgct gcataacatt cagtctgaca ttctggaact ggaaaaccgc gccgttgcgg
     9361 gtgtgaacat cgaagagttt tcccgtagcg ataacaaacg cccgcttgat ccactggata
     9421 gcagtatcac attccacgtt tgccatagcc cgcagcgtga agttgaagtt ttacacgatc
     9481 gcttgctggc gatgctggag gaagccccga cacttactcc gcgcgacatc atcgtgatgg
     9541 tggctgatat cgacagctac agtccgttta ttcaggcagt gtttggtagc gcacctgcgg
     9601 atcgttacct gccttacgcc atttccgacc gtcgtgcgcg gcagtcacat ccggtactgg
     9661 aagcgtttat cagcctgtta tcactgcctg acagtcgctt tgtgtcagag gatgtgctgg
     9721 cgctgctgga tgtgccggtg ctggcggcgc ggtttgacat caccgaagaa gggctgcgtt
     9781 atttacgcca gtgggtcaac gaatccggca ttcgttgggg gatagatgac gacaacgttc
     9841 gcgagctgga actccccgcc accggacaac acacctggcg atttggcctg acgcgtatgt
     9901 tgctgggcta cgcgatggag agcgcgcagg gcgagtggca atcggttcta ccttatgatg
     9961 aatcgagcgg cttaattgca gaactggtgg ggcatctggc ttcactgcta atgcagctaa
    10021 acatctggcg tcgcgggctg gcacaggagc gtccgctaga agagtggttg ccggtttgtc
    10081 gcgatatgct caacgccttc ttcctgccgg atgcggaaac cgaagcggcg atgacgctga
    10141 tcgaacaaca atggcaggcg attatcgccg aaggtttagg tgcgcagtat ggcgacgcgg
    10201 tgccgctgtc actattgcgt gatgaactgg cacagcgtct ggatcaagaa cgtatcagcc
    10261 agcgttttct cgccggaccg gttaatattt gtactctgat gccaatgcgt tcaattccgt
    10321 tcaaagtggt ttgcctgctg ggaatgaacg acggcgttta tccacgtcag cttgcgccat
    10381 tgggctttga cctgatgagc cagaaaccga agcgtggcga ccgtagccgt cgcgatgacg
    10441 accgctacct gttcctggaa gcgttaattt ccgcgcagca aaaactctat atcagctata
    10501 tcgggcgttc cattcaggat aacagtgaac gtttcccgtc ggtactggtg caggaactga
    10561 tcgactacat cgggcaaagt cattatctac cgggcgatga agcgctcaac tgtgatgaaa
    10621 gcgaggcaag ggtaaaagcg catcttactt gcctccatac ccggatgccg tttgatccgc
    10681 aaaactacca gccaggcgaa cgacaaagct atgctcgtga atggctacct gcggccagcc
    10741 aggctggtaa agcacattct gaatttgttc agccgctgcc gtttacctta ccggaaaccg
    10801 tgccgctgga aacgctacaa cgattctggg cacatccggt gcgggcgttc ttccagatgc
    10861 gtttgcaggt gaacttccgt actgaagaca gcgaaatccc agacaccgag ccatttattc
    10921 tggaaggact tagccgttat caaatcaatc agcagttatt gaatgcactg gttgagcagg
    10981 atgatgccga acgcttgttc cgccgcttcc gggcggcagg ggatttaccg tatggcgctt
    11041 ttggtgaaat tttctgggaa acacagtgcc aggagatgca gcagcttgcc gacagagtca
    11101 ttgcctgtcg ccagccgggg cagagtatgg aaattgatct cgcctgcaac ggtgtgcaga
    11161 taactggctg gttgccgcag gtgcagccgg atggcctgtt gcgctggcgt ccctctttat
    11221 taagtgtggc gcagggaatg caactttggc tggaacacct tgtctactgt gccagcggtg
    11281 gtaatggcga aagtcgcctt tttctacgca aagacggtga gtggcgtttt ccgccgcttg
    11341 cagccgaaca ggctttgcat tacctctcac aactgattga ggggtatcgt gaaggaatgt
    11401 ccgcgccatt gctggtgtta cctgaaagtg gcggcgcgtg gctaaaaacc tgttatgacg
    11461 cgcaaaacga tgccatgctg gatgacgatt ccacgttgca aaaagcccgt acgaaattcc
    11521 ttcaggctta cgaaggcaac atgatggtgc gtggcgaagg tgatgatatc tggtatcaac
    11581 ggctctggcg gcaattaaca ccagagacaa tggaggccat cgttgaacag tcgcaacgtt
    11641 tcctgttacc gctgtttcgc tttaatcagt catgagtgtt gtataaaaat tgcgcaatct
    11701 atccgcttac tttatgatgc gcaccagtca cggactgatg gttatataaa cataggctga
    11761 ctcgtgcagc acaagattaa attctggcag atgatttgcg ttaacctgtt gaatctgtcc
    11821 agaaaataaa gttgattatg aggtccgtga atgccccgca gcacctggtt caaagcatta
    11881 ttgttgtttg ttgccctctg ggcaccatta agtcaggcag aaacgggatg gcagccgatt
    11941 caggaaacca tccgtaaaag tgataaagat aaccgccagt atcaggctat acgtctggat
    12001 aacggtatgg tggtcttgct ggtttctgat ccgcaggcag ttaaatcgct ctcggcgctg
    12061 gtagtgcccg ttgggtcgct ggaagatccc gaggcgtacc aggggctggc acattacctt
    12121 gaacatatga gtctgatggg gtcgaaaaag tacccgcagg ctgacagtct ggccgaatat
    12181 ctcaaaatgc acggcggtag tcacaatgcc agcactgcgc cgtatcgcac ggctttctat
    12241 ctggaagttg agaacgacgc cttgcccggt gcggtagacc gcatggccga tgctattgca
    12301 gaacccttgc tcgacaagaa atacgccgaa cgtgagcgta atgcagtgaa cgctgaatta
    12361 accatggcgc gtacgcgtga cgggatgcgc atggcacagg tcagcgcaga aaccattaac
    12421 ccggcacacc ccggttcaaa gttttctggt ggtaacctcg aaactttaag cgacaaacct
    12481 ggtaatccgg tgcagcaggc gctgaaagat ttccacgaga agtactattc cgccaatttg
    12541 atgaaggcgg ttatttacag taataaaccg ctgccggagt tggcaaaaat ggcggcggac
    12601 acctttggtc gcgtgccgaa caaagagagc aaaaaaacgg aaatcaccgt gccggtagtc
    12661 accgacgcgc aaaagggcat tatcattcat tacgtccctg cgctgccgcg taaagtgttg
    12721 cgcgttgagt ttcgcatcga taacaactca gcgaagttcc gtagtaaaac cgatgaattg
    12781 attacctatc tgattggcaa tcgcagccca ggtacacttt ctgactggct gcaaaagcag
    12841 ggattagttg agggcattag cgccaactcc gatcctatcg tcaacggcaa cagcggcgta
    12901 ttagcgatct ctgcgtcttt aactgataaa ggcctggcta atcgcgatca ggttgtggcg
    12961 gcaattttta gctatctcaa tctgttacgt gaaaaaggca ttgataaaca atacttcgat
    13021 gaactggcga atgtgctgga tatcgacttc cgttatccgt cgatcacccg tgatatggat
    13081 tacgtcgaat ggctggcaga taccatgatt cgcgttcctg ttgagcatac gctggatgca
    13141 gtcaatattg ccgatcggta cgatgctaaa gcagtaaagg aacgtctggc gatgatgacg
    13201 ccgcagaatg cgcgtatctg gtatatcagc ccgaaagagc cgcacaacaa aacggcttac
    13261 tttgtcgatg cgccgtatca ggtcgataaa atcagcgcac aaactttcgc cgactggcag
    13321 aaaaaagccg ccgacattgc gctctctttg ccagagctta acccttatat tcctgatgat
    13381 ttctcgttga ttaagtcaga gaagaaatac gaccatccag agctgattgt tgatgagtcg
    13441 aatctgcgcg tggtgtatgc gccaagccgt tattttgcca gcgagcccaa agctgatgtc
    13501 agcctgattt tgcgtaatcc gaaagccatg gacagcgccc gcaatcaggt gatgtttgcg
    13561 ctcaatgatt atctcgcagg gctggcgctt gatcagttaa gcaaccaggc gtcggttggt
    13621 ggcataagtt tttccaccaa cgctaacaac ggccttatgg ttaatgctaa tggttacacc
    13681 cagcgtctgc cgcagctgtt ccaggcattg ctcgaggggt actttagcta taccgctacg
    13741 gaagagcagc ttgagcaggc gaagtcctgg tataaccaga tgatggattc cgcagaaaag
    13801 ggtaaagcgt ttgagcaggc gattatgccc gcgcagatgc tctcgcaagt gccgtacttc
    13861 tcgcgagatg aacggcgtaa aattttgccc tccattacgt tgaaagaggt gctggcctat
    13921 cgcgacgcct taaaatcagg ggctcgacca gagtttatgg ttatcggcaa catgaccgag
    13981 gcccaggcaa caacgctggc acgcgatgtg caaaaacagt tgggcgctga tggttcagag
    14041 tggtgtcgaa acaaagatgt agtggtcgat aaaaaacaat ccgtcatctt tgaaaaagcc
    14101 ggtaacagca ccgactccgc actggcagcg gtatttgtac cgactggcta cgatgaatac
    14161 accagctcag cctatagctc tctgttgggg cagatcgtac agccgtggtt ctacaatcag
    14221 ttgcgtaccg aagaacaatt gggctatgcc gtgtttgcgt ttccaatgag cgtggggcgt
    14281 cagtggggca tgggcttcct tttgcaaagc aatgataaac agccttcatt cttgtgggag
    14341 cgttacaagg cgtttttccc aaccgcagag gcaaaattgc gagcgatgaa gccagatgag
    14401 tttgcgcaaa tccagcaggc ggtaattacc cagatgctgc aggcaccgca aacgctcggc
    14461 gaagaagcat cgaagttaag taaagatttc gatcgcggca atatgcgctt cgattcgcgt
    14521 gataaaatcg tggcccagat aaaactgctg acgccgcaaa aacttgctga tttcttccat
    14581 caggcggtgg tcgagccgca aggcatggct attctgtcgc agatttccgg cagccagaac
    14641 gggaaagccg aatatgtgca tcctgaaggc tggaaagtgt gggagaacgt cagcgcgttg
    14701 cagcaaacaa tgcccctgat gagtgaaaag aatgagtgat gtcgccgaga cactagatcc
    14761 tttgcgcttg cccttacagg gcgagcgcct gattgaagcc tctgccggca caggcaaaac
    14821 ctttacgatt gcggcgctct atttgcgcct gttacttgga ctaggcggtt ccgccgcctt
    14881 tccccgcccg ctgatcgttg aagaactgct ggtggtcacc tttaccgagg ctgccacggc
    14941 agaattgcgc ggtcgtatcc gtagcaatat ccacgagttg cgcatcgcct gtctgcgtga
    15001 aaccaccgac aatccactgt acgaacgcct gctggaagag atcgacgata aagcgcaagc
    15061 cgcgcagtgg ttgttgttag ccgaacggca gatggatgaa gcggcagtct ttactattca
    15121 cggcttttgc cagcgcatgc tcaacctgaa tgcctttgaa tccggcatgc tgtttgagca
    15181 gcagctgatt gaagatgagt ctctgctacg ctaccaggcc tgcgccgatt tctggcgtcg
    15241 ccactgctac ccgctgccgc gtgaaatagc ccaggtcgtc tttgaaacct ggaaagggcc
    15301 gcaggcgttg ctgcgcgata ttaatcgtta tctgcaaggc gaagcgccgg ttatcaaagc
    15361 accgccgccc gatgatgaaa cgctggcttc ccgccacgcg caaattgtgg cgcgtattga
    15421 tacggtaaaa cagcagtggc gcgacgcagt gggtgaactg gatgcgctga tcgaatcttc
    15481 tggtattgat cgacgcaagt ttaaccgtag caatcaggct aaatggatcg aaaagatcag
    15541 cgcttgggca gaagaagaga caaacagtta tcagttgccg gagtcgctgg aaaaattctc
    15601 ccagcgtttc ttagaagatc gcacgaaggc cgggggggaa accccgcgac atccactgtt
    15661 tgaggtgatc gatcaactgc ttgcagaacc attgtcgatc cgcgatctgg tgatcacccg
    15721 agcattggct gagatccgcg aaacagtagc gcgtgaaaaa cgccgccgtg gcgaattggg
    15781 ttttgatgac atgttaagtc ggctcgattc cgcgctgcgt agcgaaagcg gcgaggtgtt
    15841 ggcagcggcg atccgtacgc gattcccggt ggcaatgatc gatgaatttc aggataccga
    15901 cccccagcag taccgaattt ttcgccgtat ctggcaccat cagccggaaa ccgcattgtt
    15961 gctgattggc gacccgaagc aggccatata tgcattccgg ggtgcggata tcttcactta
    16021 tatgaaggcg cgtagtgaag ttcacgccca ctacacttta gacaccaact ggcgctccgc
    16081 acctggaatg gtgaacagtg tgaacaagct tttcagccag actgatgacg cgttcatgtt
    16141 tcgcgaaata ccgtttattc cagtgaaatc agccgggaaa aatcaggcgt tacgttttgt
    16201 atttaaaggt gaaacgcagc ctgcgatgaa aatgtggctg atggaaggcg aaagctgcgg
    16261 cgttggcgac tatcaaagta ccatggcgca ggtatgtgct gcgcaaatcc gcgactggct
    16321 acaagccgga cagcggggcg aagcgttgct gatgaacgac gacgacgcgc gtccggtgcg
    16381 tgcttcggac atcagcgtgc tggtgcgcag ccgccaggag gccgcccagg tgcgcgatgc
    16441 cttaacgttg ctggaaatcc cttccgttta cctttcgaac cgcgacagtg tttttgaaac
    16501 tctggaagcg caggaaatgc tttggttgtt gcaggcggtg atgacgcccg aacgtgagaa
    16561 caccctgcgc agtgcgctgg caacgtcaat gatggggctg aatgcgctgg atattgaaac
    16621 gctgaataat gacgaacatg cgtgggatgc ggtagtcgaa gagttcgatg gttatcggca
    16681 aatctggcgc aaacgtggcg ttatgccgat gctgcgggcg ctgatgtcgg cgcgtaacat
    16741 tgcagaaaac ttgctggcaa cggcaggcgg tgagcggcgt cttaccgata tcttgcatat
    16801 cagcgaactg cttcaagaag ccggaacgca gctggaaagt gaacatgcgc tggtacgctg
    16861 gttatcgcaa catatcctcg agccagacag taatgcctcc agccaacaaa tgcgcctcga
    16921 aagtgataaa catctggtgc agattgtcac gatccataaa tcgaaagggc tggaatatcc
    16981 gctggtctgg ttgccattta ttaccaattt ccgcgtccag gatcaggcgt tttatcacga
    17041 tcgccactcg tttgaggcag ttttggatct taatgctgcg ccagaaagcg tcgacctcgc
    17101 ggaggtcgaa cgtctggcgg aagatctgcg tttgctttac gtggcgctga cgcgttcggt
    17161 ttggcattgc agtctcggcg ttgcaccgct ggtgcgccgt cgtggcgata aaaaaggtga
    17221 caccgacgtc caccaaagtg cgctcgggcg tttgctgcaa aaaggggaac cgcaagatgc
    17281 ggcagggctt cgcacctgta ttgaagcgtt atgcgatgat gatattgcct ggcaaacggc
    17341 acaaactggt gataaccagc cctggcaggt taatgatgct ttaactgcag aactgaatgc
    17401 gaggacacta caacgattgc ccggcgataa ctggcgtgtc accagctact ccggcttgca
    17461 gcagcgtggt cacggtatcg ctcaggatct gatgccacgg ctggatgttg atgccgcagg
    17521 cgtggtcagc gtcgttgaag aaccgacgtt aacaccgcat cagttcccgc gcggtgcgtc
    17581 accggggaca ttcttgcaca gtttgtttga agacctcgat tttacccagc cggttgaccc
    17641 gaactgggta caggaaaaac tggaactcgg cggctttgaa tcgcagtggg aaccggtgtt
    17701 gaccgagtgg atcacggctg tcctccaggc acctctcaat gaaacgggtg ttagcctgag
    17761 tcagctttcc gaccgcgata aacaggtgga gatggagttt tacctgccga ttagcgaacc
    17821 gctcatcgcc agccagcttg atgcactaat tcgccagttt gacccgctat ccgctggctg
    17881 cccgccgctg gagttcatgc aggtacgcgg catgttaaaa ggctttatcg acctggtatt
    17941 ccgccacgaa gggcgttatt acctgctcga ctataaatcc aactggctgg gtgaagacag
    18001 ttcggcttac acccaacagg ctatggcagc agcgatgcag gcacaccgct atgatctgca
    18061 atatcagctt tataccctgg cactgcatcg ttatctgcgc catcgcattg ctgattacga
    18121 ctatgatctt cactttggcg gcgttatcta tctgttcctg cgtggcgttg ataaagaaca
    18181 tccgcaacaa gggatttaca caacccgacc caacgccggg ttgattgccc tgatggatga
    18241 gatgtttgcc ggtatgaccc tggaggaggc gtaatgaaat tgcaaaagca attactggaa
    18301 gctgtggagc acaaacagct acgcccgctg gatgtgcaat ttgccctgac cgtggcggga
    18361 gatgaacatc ctgctgtcac cctcgcggcg gcactgttaa gtcatgatgc cggagaggga
    18421 cacgtctgtt tgccgctttc acgactggaa aataacgagg cgtcgcatcc gctgttggcg
    18481 acctgtgtca gtgaaatcgg tgagctacaa aattgggaag aatgcttgct ggcttctcaa
    18541 gcggtcagca ggggagatga acccacgccg atgatcctct gtggcgatcg tctttatttg
    18601 aatcgcatgt ggtgtaacga gcggacggta gcgcgcttct tcaatgaggt gaatcatacc
    18661 attgaggttg atgaagctct actggcgcaa accctggaca aactttttcc agtaagcgat
    18721 gaaattaact ggcaaaaagt tgcagcggca gtggcgctga cgcggcggat ctcggtgatt
    18781 tccggcggcc caggcaccgg taaaacgacc accgtggcga agttgctggc agcgttaatt
    18841 caaatggccg acggcgaacg ctgccgtatc cgtctggctg caccaacggg taaagctgcc
    18901 gcgcgcttaa ccgaatctct cggcaaggct ttgcgacagt taccgctgac cgatgaacaa
    18961 aagaaacgca ttccggaaga tgccagcact ttgcaccgat tgctgggcgc gcagccgggt
    19021 agccagcgtt tacgtcatca tgccggtaac ccgctgcatc ttgatgtgct ggtggtagat
    19081 gaagcgtcaa tgatcgatct gcctatgatg tcgagactga tcgacgcctt gccagatcat
    19141 gcgcgagtga tctttctcgg cgatcgtgat caactggcct cggttgaggc tggggctgtg
    19201 ctgggcgata tctgcgctta tgccaacgcg ggctttaccg ccgagcgtgc caggcagcta
    19261 agccgcctga cggggactca cgttccggca ggaaccggca cagaagcggc atctttgcgc
    19321 gacagtctct gcctgctgca aaaaagctat cgtttcggca gcgattctgg cattggtcag
    19381 ttagctgcgg cgatcaaccg tggtgataaa acggcagtga aaaccgtttt tcagcaggat
    19441 tttactgata tcgaaaaacg acttttacag agcggcgaag attatattgc gatgcttgag
    19501 gaagctcttg cgggttacgg acgttatctg gatctgctgc aagcgcgtgc cgagccggat
    19561 ttaatcattc aggcgttcaa tgagtaccag cttttgtgcg ccctgcggga agggccgttt
    19621 ggcgtggctg gactgaatga gcgaattgag cagtttatgc aacagaagcg caaaattcat
    19681 cgtcatccgc actctcgttg gtacgaaggc cgaccggtga tgattgcccg taatgacagc
    19741 gcgcttgggt tgtttaatgg cgatatcggt attgcgctgg atcgcgggca ggggacgcgc
    19801 gtctggtttg cgatgccgga cggcaatatt aagtctgtgc aaccgagtcg cttgccagag
    19861 cacgaaacta cgtgggcgat gacggtacat aaatcgcagg gatcggagtt cgaccatgcg
    19921 gcgttgattt taccgagcca acgcacgccg gtagtaacgc gagagctggt atacaccgcg
    19981 gtgacccgcg cgcgtcgtcg tctgtcgctg tatgccgatg agcgtatatt aagtgcggca
    20041 atcgccactc gtactgagcg gcgcagtggt ctggcggcgt tgtttagttc gcgggaataa
    20101 acgtaattgc cggatgcgac gcacgagtgt tacgcctgcc gcatccggtg attttcatcg
    20161 cttaccctaa atccgccatc aacactttgg atttacgctg gtagttgtac aactgctttt
    20221 tgctctcggg cagtaaatca atatccaccg gggtaaatcc acgttcctgg aaccagtgaa
    20281 tactgcgcgt ggtcagcaca aacaatttgc ttaagccgct ctgcttcgcc tgagcggcaa
    20341 tgcgttccag cagaacctcg ccccgtgatg aactgcggta atccgggtga actgccacac
    20401 aggccatttc cccaatcttc tcttccggga acggatagag cgcggcgcag gcaatagtcg
    20461 tgttatcgcg ctgaataatg gtgaatttgt cgatttccat ctccagctgc tcgcgagaac
    20521 ggcgcaccag aataccttgc tgctccagcg ggcgaatcaa ctccagaata ccgccaatat
    20581 cgttgattgt tgcgcgacga atctgctcgg cgctttccat cacaatctgc gtaccgatac
    20641 cgtcgcgtga gaacaactct tgcaacagcg cgccatcttc ctgataactg attaaatgac
    20701 agcggcgcac gccgctgcgg caggctttca ctgcgccacg caaaaagcgc accgtaccgg
    20761 agttgtaatc gcctttctct tcctgggctt ctacccgtgc ttgcgcttcg ttagggaaaa
    20821 gttcggagac aatatcaccg tcgtcattag taacgccctg ggaagagcaa aaaccaatca
    20881 tcttttcagc tttcagtttg atggctagtt gagtggcaat ctcttcagag gtcagattaa
    20941 agctctcgcc agtgactgaa acagcgaccg gccccattag cactattgca ccgctttcca
    21001 gttgacgatg gatcgcgtct tcatcaatcc gccggatacg cccgctatgg cagtaatcca
    21061 cgccgtcatc gacgcccagc ggctgggcaa taataaaatt gccactaacg acgttgatat
    21121 gcgcgccctg cagcggcgtg ttattgagac tcattgacag gcgagcagta atatccagtt
    21181 gcaatgttcc cgcagcctgc ttcaccagtt ccagtgtttt ggcgtcggtc acacgtatat
    21241 tcttgtgata cagcggttcg tggtgatgcg cagccagatt tgcgtcgatc tgcggacgtg
    21301 cgccatagac caccaccaga cggatgccga ggctgtgcaa caacccgata tcattaacga
    21361 tactggagaa attctcatgc tcaatggctt caccgccgag catgatgaca aacgtttttc
    21421 cccggtgggt attgatatag ggaaccgaat ggcggaatcc ctcgaccagc tcggttttac
    21481 gttcctttac cacggcacac ccctttgcat gattatttga aattattgta tttttattct
    21541 gttttttcgc agggtgcaag tgtaaatttt atgcgagagc gactttttta tcagtaatgc
    21601 cgtgaatata aaaagaatgt ttaccgttta ttagatgaca gattatgcgt ctttcgctaa
    21661 agtttccggt caaattagtc gtttacttgt tacacagctt agatttcttt tgctcgggag
    21721 aggcatgtca ggatccaaca ctgcaatcag ccgtcgtcgt ttactgcaag gcgcgggcgc
    21781 tatgtggcta ttgagcgtaa gtcaggtcag cctggctgcg gttagccagg tcgtggcggt
    21841 gcgcgtctgg cctgcgtcca gctacacccg cgtgacggta gaatcaaatc gtcagctgaa
    21901 atataagcag ttcgcgttga gtaaccctga acgcgtggtg gtggatatcg aagatgtaaa
    21961 cctgaactcg gtactcaagg ggatggcggc acaaattcgt gcagacgacc cgttcatcaa
    22021 gtcggcgcgc gtcgggcaat ttgacccgca aaccgtacgc atggtttttg aattaaagca
    22081 aaacgtaaaa ccgcagctgt ttgcccttgc gccggtcgcc gggtttaaag agcgtctggt
    22141 gatggacctc tatccggcca atgcacagga tatgcaggac ccgctgctgg cgctgctgga
    22201 ggattacaac aaaggcgacc tcgaaaagca ggtgccgcca gcgcaaagtg gtccacaacc
    22261 gggtaaagca gggcgcgatc gtccgattgt cattatgctt gaccctggcc acggtggcga
    22321 agattccggt gcggtgggga aatacaaaac gcgcgaaaaa gatgtggtat tgcaaatagc
    22381 tcgccgtttg cgctctctga tcgagaaaga gggcaatatg aaggtgtaca tgacgcgcaa
    22441 tgaagacatc ttcattccgt tgcaagtgcg cgtagcaaaa gcccagaaac agcgtgctga
    22501 cctgtttgtc tctatccatg ccgacgcctt taccagtcgt cagccgagcg gttcctcggt
    22561 gtttgcgctc tcaaccaaag gcgcaaccag tactgcggca aaatatctgg cacaaaccca
    22621 gaacgcctcg gacttgattg gtggcgtgag caaaagcggt gaccgctatg tcgaccacac
    22681 catgttcgat atggtgcagt cgctgactat tgctgacagc cttaagtttg gtaaagcggt
    22741 actgaataag ctcggaaaaa tcaacaagct gcataaaaat caagttgaac aggccgggtt
    22801 tgccgtacta aaggcaccag atattccctc cattctggtc gaaacggcgt ttatcagtaa
    22861 cgttgaggaa gagcgtaaac tgaaaacggc gactttccag caggaagttg cggagtctat
    22921 tcttgcgggg attaaagcgt attttgccga tggggcaacg ctggcgagaa ggggatgata
    22981 aaaaggcgct gaatggcgct ttttttatgg gagatcagaa acaaaaaaac acccgttagg
    23041 gtgttcaata attggttgcg ggggccggat ttgaaccgac gaccttcggg ttatgagccc
    23101 gacgagctac caggctgctc caccccgcgt caccgtactg cttttacttc atcaaatttg
    23161 attggttgcg ggg
//
LOCUS       DAJTNM010000058        22776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTNM010000058 DAJTNM010000000
VERSION     DAJTNM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.058"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..184)
                     /locus_tag="OO172_002310"
     CDS             complement(<1..184)
                     /locus_tag="OO172_002310"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5512478.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVK"
     gene            complement(325..2358)
                     /gene="metG"
                     /locus_tag="OO172_002311"
     CDS             complement(325..2358)
                     /gene="metG"
                     /locus_tag="OO172_002311"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5512479.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2490..3599
                     /gene="apbC"
                     /locus_tag="OO172_002312"
     CDS             2490..3599
                     /gene="apbC"
                     /locus_tag="OO172_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5512480.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3861..4142
                     /locus_tag="OO172_002313"
     CDS             3861..4142
                     /locus_tag="OO172_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5512481.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4435..4977
                     /locus_tag="OO172_002314"
     CDS             4435..4977
                     /locus_tag="OO172_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5512482.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5058..5732
                     /locus_tag="OO172_002315"
     CDS             5058..5732
                     /locus_tag="OO172_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5512483.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5748..8228
                     /locus_tag="OO172_002316"
     CDS             5748..8228
                     /locus_tag="OO172_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5512484.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8244..9277
                     /locus_tag="OO172_002317"
                     /pseudo
     CDS             8244..9277
                     /locus_tag="OO172_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9359..9697)
                     /gene="rcnB"
                     /locus_tag="OO172_002318"
     CDS             complement(9359..9697)
                     /gene="rcnB"
                     /locus_tag="OO172_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5512485.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9916..10758)
                     /gene="rcnA"
                     /locus_tag="OO172_002319"
     CDS             complement(9916..10758)
                     /gene="rcnA"
                     /locus_tag="OO172_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR5512486.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHEHHQDHEHHHDQGHHHHHEHGEYQDAHA
                     RAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVS
                     FSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFM
                     GIMR"
     gene            10879..11151
                     /gene="rcnR"
                     /locus_tag="OO172_002320"
     CDS             10879..11151
                     /gene="rcnR"
                     /locus_tag="OO172_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5512487.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11243..11339
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11374..12162
                     /gene="thiM"
                     /locus_tag="OO172_002321"
     CDS             11374..12162
                     /gene="thiM"
                     /locus_tag="OO172_002321"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5512488.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12159..12959
                     /gene="thiD"
                     /locus_tag="OO172_002322"
     CDS             12159..12959
                     /gene="thiD"
                     /locus_tag="OO172_002322"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5512489.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13024..13842
                     /locus_tag="OO172_002323"
     CDS             13024..13842
                     /locus_tag="OO172_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5512490.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13894..14640
                     /locus_tag="OO172_002324"
     CDS             13894..14640
                     /locus_tag="OO172_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5512491.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14614..15579)
                     /locus_tag="OO172_002325"
     CDS             complement(14614..15579)
                     /locus_tag="OO172_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5512492.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15576..16579)
                     /locus_tag="OO172_002326"
                     /pseudo
     CDS             complement(15576..16579)
                     /locus_tag="OO172_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16576..17052)
                     /locus_tag="OO172_002327"
                     /pseudo
     CDS             complement(16576..17052)
                     /locus_tag="OO172_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            17206..18258
                     /gene="fbaB"
                     /locus_tag="OO172_002328"
     CDS             17206..18258
                     /gene="fbaB"
                     /locus_tag="OO172_002328"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5512493.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18566..19420
                     /gene="gatY"
                     /locus_tag="OO172_002329"
     CDS             18566..19420
                     /gene="gatY"
                     /locus_tag="OO172_002329"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5512494.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19449..20716
                     /gene="gatZ"
                     /locus_tag="OO172_002330"
                     /pseudo
     CDS             19449..20716
                     /gene="gatZ"
                     /locus_tag="OO172_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            20726..21178
                     /gene="gatA"
                     /locus_tag="OO172_002331"
     CDS             20726..21178
                     /gene="gatA"
                     /locus_tag="OO172_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5512495.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21209..21493
                     /gene="gatB"
                     /locus_tag="OO172_002332"
     CDS             21209..21493
                     /gene="gatB"
                     /locus_tag="OO172_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5512496.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21497..22776
                     /locus_tag="OO172_002333"
                     /pseudo
     CDS             21497..22776
                     /locus_tag="OO172_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
BASE COUNT         5903 a         5287 c         5752 g         5834 t
ORIGIN      
        1 tcttcacctt ctccgcaatc agcttcagtt ccgctttttc cgccgccgca gcatctgaaa
       61 aactttttat ctgactttgc cctttggtgc caacttttcc ccaactgata tgcaactcgt
      121 ttccctgttg ctccaccgtc cagaatttat gtgatttttc gtcctgatag ataaagtgtc
      181 tcattctgca tccctgtaat tttatccagt tgaatgcaga tgctaccagt atttatacgg
      241 gttagagaga gacaaaatcg caagaaaaca actttaggaa tttataaaga aaaggcgctg
      301 tcgatgcagc gccttgaagg gggattattt cacctgatga cccggtttag caccggcatc
      361 cgggcttagc aggaaaatat ctttcccgcc aggaccggca gccatcacca tgccttcaga
      421 gataccgaag cgcattttac gtggtgccag gttagccacc ataatggtgt gacgaccaat
      481 cagtgcctgc ggatccgggt aagcagaacg aataccggag aagacattgc gtttttcacc
      541 gccgagatcc agcgtcaggc gcagcagttt gtcagaacct tcaacaaact ctgcgttttc
      601 gatcagcgcc acgcgcaggt caactttggc gaagtcgtca aaggtgatgg tttcctgaat
      661 cggatcatct gccagtgggc cagtaactgg cgcggtagtg gctttcactt cttctttaga
      721 ggcttccacc agagcttcaa cctgtttcat atcgatgcgg ttatacagcg ccttgaacgg
      781 attcactttg tggcccagca gcggttgctg gataccatcc caggtcagtt ccgtattgag
      841 gaaggcttct gcacgctcgg tcagtttcgg cagtaccggc ttcaggtaag tcatcagcac
      901 gcggaacagg ttgatgccca ttgagcaaat cgcctgcagg tcagcatcgc gaccttcctg
      961 ttttgcgact acccacggag cctgctcgtc gacatagcgg ttagccaggt cagccagtgc
     1021 catgatttcg cgcactgctt taccaaattc acggctttcc cacgcttcac caatcacttc
     1081 agcggcatcg gtaaaggttt tgtacagctc agggtcagcc agctcgcttg ccagcacacc
     1141 gtcaaaacgc ttgttgataa agcccgcatt acgggaggcc aggttaacca ctttgttcac
     1201 gatatcggca ttcacacgct gaacgaaatc ttccaggttg agatcgatat catcaatgcg
     1261 cgaagagagt ttcgcagtgt agtagtaacg caggctgtca gcgtcaaaat gattcagcca
     1321 ggtgctggct ttaataaagg tgccgcgaga cttggacatc ttcgcgccgt tcaccgtcac
     1381 atagccatga acaaacaggt tggtcggctt gcggaagttg ctgccttcca gcatggcagg
     1441 ccagaacagg ctgtggaagt aaacgatatc tttaccgatg aagtggtaca gctcggcggt
     1501 ggagtctttc ttccagtatt catcgaagct tacgctgtcg ccgcgcttgt cgcacagatt
     1561 cttgaaagaa cccatgtagc caatcggtgc gtccagccag acgtagaaat atttgcccgg
     1621 cgcgttcgga atttcaaaac caaagtatgg cgcgtcgcgg gagatatccc actgttgcag
     1681 gccggattca aaccactcct gcattttgtt tgccacctgc tcctgcaacg caccgctgcg
     1741 ggtccatgcc tgcagcattt cgctgaaaga gggcagatca aagaagaagt gttcagaatc
     1801 acgcatcact ggtgtagcac cagaaaccac cgatttcggc tcgatcaatt cagtcgggct
     1861 gtaggtcgcg ccgcagactt cgcagttatc gccgtattga tccggggatt tacatttcgg
     1921 gcaggtgcct ttcacaaaac ggtccggcag gaacatgcct ttttccggat cgtacagctg
     1981 agagatggtg cggtttttaa taaaaccgtt ttctttcagg cgagagtaga taagttctga
     2041 caactggcgg ttctcttcgc tgtgcgtcga gtgatagttg tcatagctga tgttaaagcc
     2101 tgcgaaatca gtctgatgct cctgactcat ttcgccaatc atctgctccg gggtgatacc
     2161 gagctgctga gctttcagca tgatcggtgt accgtgggca tcgtcggcgc agatgaagtt
     2221 gacctcgtgg ccgcgcattc gctggtaacg gacccagaca tcagcctgga tgtgctccag
     2281 catatggccg aggtggattg agccgttagc gtacggcagt gcgcacgtca ccagaatttt
     2341 cttcgcgact tgagtcatag taggcattac ttcttaatag tgaaaagggg ttagatagta
     2401 ccaaatggga aaatgttaag taagtacttc tctatattct tcaggtaaac tatcagctga
     2461 tggtttatct atacaaaaaa ggagtcggga tgaacgaaca atcccaggcc aaatcaccag
     2521 aagccttgcg cgcaatggtc gccgggacac tggccaattt tcagcaccca accctgaagc
     2581 ataacctcac cacgctgaaa gcgttgcacc atgtcgcctg gatggacgac acgttgcatg
     2641 tggaactggt tatgccgttt gtctggcata gcgcgttcga agagttaaaa gagcaatgta
     2701 gcgccgaact gctgcgcatc accggggcga aagctatcga ctggaaactg tcgcataaca
     2761 tagccactct gaaacgcgtg aagaatcagc cgggaattaa cggtgtgaag aacattatcg
     2821 cggtgagttc cggtaaaggc ggcgtgggta aatcgtccac ggcggtaaac ctggcgctgg
     2881 cactggcggc ggaaggggcg aaagtcggca ttctggacgc cgatatctac ggcccatcaa
     2941 ttcccaccat gctgggcgca gaaaatcagc gtccaacttc gccggacggt acccacatgg
     3001 caccgattat gtctcatggc ctggcaacca attcaattgg ttatctggtc actgacgaca
     3061 atgcgatggt gtggcgtgga ccgatggcga gcaaggcgtt aatgcagatg ttgcaggaaa
     3121 ccttgtggcc ggatctcgac tatctggtgc ttgatatgcc gccaggcacc ggtgacattc
     3181 aattgacgct ggcgcaaaac attccagtaa ctggtgcagt cgtggtcacc accccgcagg
     3241 acatcgcgtt gatcgatgcg aaaaaaggca ttgtgatgtt cgagaaagtc gaggttccgg
     3301 tgctcggtat tgtcgagaac atgagtgtgc atatttgcag taactgtggt caccatgaac
     3361 cgattttcgg taccggtggt gcagagaaac tggctgaaaa ataccataca cagctgctgg
     3421 ggcagatgcc gttacacatc tccttacgtg aggatctcga taaaggcacg ccaacggtta
     3481 tcagccgtcc ggagagcgaa tttaccgcta tctaccgcca gctggctgac cgcgttgcag
     3541 ctcagctcta ctggcagggt gaagtcattc ctggcgagat ttccttccgc gcggtctaac
     3601 gcctattaac cattctggtt attcttcata catcgcagag atgttatatt gatgcggtgt
     3661 atttgttttt gccgcattaa tgaacactta tttaatactg cgaatattct gcggaccttc
     3721 gtccgaaact ctacaaagtc gagtgatcgt acaggcaatg cttcattatc atccttcgtg
     3781 ctgtgtagtg gatgtacagc tatagtttgt tatgcactca ttctatccag gcataacctt
     3841 tactacaggg ataacgttca atgaataagt attggctgtc aggaattatt tttcttgcct
     3901 atggattggc ttctcccgca gtctcttcag agactgccac attaacgatt aatggcagga
     3961 tcagtccccc aacatgcagt atggctatgg ttaatagtca accacaacag cattgtggtc
     4021 agttaacgta taatgttgac acccgtcatc aggtttcttc gcccgtcaaa ggtgttacga
     4081 cagaagtggt tgttgcaggt agcgatagca aacgtcgaat cgtgttaaat cgctacgatt
     4141 aatttggcaa cctgatttta attgcattta gaaagacgaa atcatttata taaatgattt
     4201 cgtctttttt attgatatca acaataccat ttacataaat ctacattaat taatagatgt
     4261 gataaatatt taatattctt aagtatttgt tgtatttata ttgcattggt taatttgttt
     4321 taaatcaata aaacaatatg tttacattgc ggcagctttt ttgatgtgag aaatttgttt
     4381 ccaagagata cagactctta acaaccaaaa ataaatttta ttaaggaaca tattatgaaa
     4441 cgttcaatta ttgctgccgc tgtcttttct tcttttttta tgagcgctgg agtatttgct
     4501 gcagacgttg ataccggaac attaaccatt aaaggaaata ttgcagaatc tccgtgtaaa
     4561 ttcgaagcgg gtggtgattc agtaagtatt aatatgccga ctgtaccaac cactgtcttt
     4621 gaaggtaaag ctaaatattc tacctatgat gatgcagtcg gtgtaaccag cagcatgtta
     4681 aaaattagct gcccgaaaga agttgctggt gtaaaactct cgttgatcac aaacgacaaa
     4741 ataaccggta acgataaggc gatagccagt agcaacgata ccgtgggtta ctatctctat
     4801 ttaggtgata acagcgatgt cctagatgtt tctgcacctt ttaacattga gagttataaa
     4861 acagcagaag gtcaatatgc tattccgttt aaagcaaaat acctgaaact gacagataac
     4921 tcagtgcaat caggtgatgt gttatcttct ctggttatgc gtgtagcgca ggattaataa
     4981 ataaaattgc aagtttctcc aggtaataaa catggccgct atccccatgg cggcttttta
     5041 atttaagagg cattaagatg aaaggattat tatctttact cattttttct atggtccttc
     5101 ctgcacatgc cggaattgtt atctacggga cgcgcattat ttacccggca gaaaataaag
     5161 aagttatggt gcagttgatg aaccagggaa atcgttcttc gctgctgcag gcgtggattg
     5221 atgatggcga tacgtcgtta ccaccagaaa aaattcaggt tcctttcatg ttaacgccac
     5281 cagtggcaaa aataggggca aattcagggc agcaagtaaa aatcaaaatt atgccgaata
     5341 aactgcccac taataaagaa agcatttttt atctgaatgt tctggatatt ccaccaaata
     5401 gtccagagca agaaggcaag aatgcactga agtttgcgat gcaaaacaga attaagttgt
     5461 tttaccggcc agcgggtatt gctccggtaa ataaagcgac ctttaaaaag ttactggtaa
     5521 atcgcagtgg caatggattg gtgataaaaa atgactcagc taattgggtg acgatttcgg
     5581 atgtcaaagc taataatgtt aaagtcaatt atgaaactat tatgattgcc cccttagaaa
     5641 gtcagagtgt taatatcaaa agtaataatg caaataactg gtatctgacc attatcaatg
     5701 accacggcaa ctatattagt gacaaaattt aattgcagga gctgcctatg ttgagaatga
     5761 ccccacttgc atcagcaatc gtagcgttat tgctcggcat tgaagcttat gcagctgaag
     5821 aaacctttga tacccatttt atgataggtg gaatgaaaga ccagcaggtt gcaaatattc
     5881 gtcttgatga taatcaaccc ttaccggggc agtatgacat cgatatttat gtcaataagc
     5941 aatggcgcgg gaaatatgag attattgtta aagacaaccc gcaagaaaca tgtttatcaa
     6001 gagaagttat caagcggtta ggcattaata gcgataactt cgccagcggt aagcaatgtt
     6061 taacatttga gcaacttgtt cagggtggga gctatagctg ggatatcggg gtttttcgtc
     6121 tcgatttcag tgtcccgcag gcttgggtgg aagaactgga aagtggctat gttccaccgg
     6181 aaaactggga gcggggtatt aatgcgtttt atacttctta ttatgtgagt cagtattaca
     6241 gcgactataa agcgtcgggt aacaacaaga gtacatatgt acgttttaac agcgggttaa
     6301 atttactgga gtggcaactg cattctgatg ccagtttcag taaaacaaat aacaatccag
     6361 gggtgtggaa aagcaatacc ctgtatctgg aacgtggatt tgcccaattt ctcggcacgc
     6421 ttcgcgtggg tgatatgtac acatcaagcg atatttttga ttctgttcgc ttcagcggtg
     6481 tgcggttgtt tcgtgatatg cagatgttgc ctaactcgaa acaaaatttt acgccacggg
     6541 tgcaggggat tgctcagagt aacgcgttgg taactattga acagaatggt tttgtggttt
     6601 atcagaaaga ggttcctcct ggcccgtttg cgattacaga tttgcagttg gccggtggtg
     6661 gagcagatct tgatgttagc gtgaaagagg cggacggctc ggtaaccacc tatctggtgc
     6721 cttatgcagc ggtgccaaat atgctgcaac ccggcgtgtc gaaatatgat tttgcggcgg
     6781 gccgtagcca tattgaaggg gcgagcaagc aaagtgattt tgtccaggca ggttatcagt
     6841 atggttttaa taacttattg acgctgtatg gcggcacgat ggttgctaat aattactatg
     6901 cgtttaccct cggaacgggt tggaacacgc gcattggcgc aatttccgtc gatgccacga
     6961 agtcgcatag taaacaagac aacggcgatg tgtttgacgg gcaaagttat caaattgcct
     7021 acaacaaatt tgtgagccaa acgtcgacgc gttttggtct ggcggcctgg cgttattcgt
     7081 cgcgtgatta ccggacattt aacgatcacg tttgggcaaa caataaagat aattatcgcc
     7141 gtgatgaaaa cgatatctat gacattgccg attattacca gaacgatttt ggccgtaaaa
     7201 atagcttttc tgccaatatg agccagtcat tgccagaagg ctggggttct gtgtccttaa
     7261 gtacgttatg gcgagattac tgggggcgta gcggcagcag taaggattat cagttgagtt
     7321 attccaataa ctggcggcgg ataagctata ccctcgcggc aagccaggcg tatggcgaga
     7381 atcatcatga agagaaacgt tttaatattt ttatatcaat tccctgtgat tggggtgatg
     7441 acgttacgac gcctcgtcgg caaatatata tgtctaactc aacgacgttt gatgatcagg
     7501 gttttgcctc aaataatacg ggattatcag gaacagtagg gagtcgggat cagttcaatt
     7561 atggtgtcaa cctgagtcat caacatcagg gaaatgaaac gacagctggg gcgaatttga
     7621 cctggaacgc gccggttgcg acagtgaatg gcagttatag tcagtcgagt acttatcgac
     7681 agactggagc cagtgtttca gggggcattg tcgcctggtc gggtggcgtt aatctggcga
     7741 accgtctttc cgaaacgttt gctgtgatga atgcgccagg aattaaagat gcttatgtca
     7801 atgggcaaaa atatcgcaca acaaaccgta atggagtggt ggtatacgac ggaatgacac
     7861 cttatcggga aaatcacctg atgctggatg tgtcgcaaag cgatagcgaa gcagaattac
     7921 gtggcaaccg gaaaattgcc gccccttatc gcggcgcggt tgtactggtt aattttgata
     7981 ccgatcagcg caagccctgg tttataaaag cgttaagagc ggatggtcaa ccattaacgt
     8041 ttggttatga agtcaatgat atccatggtc ataatattgg cgttgtcggc cagggaagcc
     8101 agttatttat tcgcaccaat gaaataccgc catcggttaa tgtagcaatt gataagcaac
     8161 aaggactttc atgcacaatc accttcggta aagagattga tgaaagtaga aattatattt
     8221 gccagtaata gtcaggtggt tctatggaaa ttcgcataat gctatttata ttaatgatga
     8281 tggttatgcc tgtgagctat gcggcatgtt atagtgagtt atctgttcag cacaatttgg
     8341 ttgttcaggg gaattttgca cttactcaaa cacaaatagc gacatatgag cataatttta
     8401 atgattcgtc atgcgtgagt acaaatacta tcacccctat gagcccatcg gatattattg
     8461 ttggacttta taacgatacc attaaattaa atttacattt tgaatggacc aataaaaaca
     8521 acatcacgtt gtcaaataat cagagcagtt tcaccagtgg ttattcggta acggtgacac
     8581 ctgcggccag taatgcaaaa gtgaatattt ctgcgggggg cggtggttca gtgatgatta
     8641 atggtgttgc gacattatcc agtgcttcat catcgacacg cgggagtgcc gcagtacaat
     8701 ttctactgtg tttattaggt ggcaagtcat gggatgcatg tgtaaatagc tacagaaatg
     8761 cattggcaca aaatgcaggt gtctattcct tcaatctgac attgtcatac aacccgataa
     8821 ccacaacctg caaacaggac gatttattaa ttactttaga cagtattccc gtttcacaat
     8881 taccagccac aggtaacaaa gcaacaataa atagtaaaaa aagggatatt attctgcgtt
     8941 gtaaaaattt attaggtcaa caaaatcaaa catcacggaa aatgcaggtg tatttatcaa
     9001 gttctgactt gttaaccaac agcgacacaa tactgaaagg tgcggaagat aatgcgtagg
     9061 atttattctt gaaagtaatg gttcgccagt cacactttta aatatcacta acagcagtaa
     9121 aggatacaca aatttaaaag aaattgcggc aaagtcaaaa cttacagata caacggtttc
     9181 aattccgata acagccagtt actacgtcta cgatacaaac aaagttaaat ctggcgcact
     9241 ggaggcaacc gcattaatca acgtgaaata cgactaataa caagcaagac gagcaagtgg
     9301 ctaataataa aaaataacaa ggtgtctgca tttccccctc atgatgaggg ggcttttttt
     9361 agcgatgata aaaaatctca ccgtcgtagg ctttaatgat tttaccgtcg gtgtcgctga
     9421 tcaacacgta cgcgccaccc atataggtcc agtgcgtccc ggcatcaggc gcgggcaggt
     9481 tacgtaactg ccactgctta atgttgtact catcggtgcg gtacatctct gggacggtat
     9541 cacctggttt gaaatgctta aagtcagcaa tgaaactact taactcatac ttagaaattc
     9601 ccgaggtatt ggtcgaaccc gcggtggctg gtgcggccca ggcggcactg gtaaccagca
     9661 aaagcacacc cagcaacatc ttatttttaa tggtcataaa ctctccactt tggggatctg
     9721 taatatcagt cgcatatctt tcctgccata caatataact gacaagcgat attttgttag
     9781 tgatcccatg atttcgtaaa aagtgtaaat gaaaggatgc cctaaaagca tccttagcga
     9841 gcagagagtc agcggaaata gctgatttaa gaatcatcct gggctgaaga taacatgcaa
     9901 gcctaaagga ttgagttatc gcattatgcc catgaagcca tgtacgccca tatacacacc
     9961 gactaagcca atcaacagac tggaaaaata gggggcgcgt ttagcgagag tgttaaatcc
    10021 gctccagcgt tttgcgacct gctgaacgct gattgctgcg ccaacgccta cggtgacaag
    10081 cgttaacgcc aggccaatgc tgaaactgac gaccagtgtt gcgcccagtg tcagggcttt
    10141 caactgaatg catatcaaca gcacggtaat tgctgccggg caggggataa ggccaccggt
    10201 taagccaaat aacaaaattt gccagttgat gacctctcta ccatcaaagc gtcgtttaat
    10261 gtcattggca tgggctcgtg catgggcatc ctgatactcg ccatgttcgt gatggtgatg
    10321 atgtccttgg tcgtggtgat gttcgtggtc ttgatgatgt tcgtgatgat ggtgatgatg
    10381 ctcataatca tgcccgtgca tattctccag ccagttgcgt tcgccgcgcc aggtacgcca
    10441 gaacatccag aacacggtgc caataatgat cactgcggaa atcagctgga gccacggttc
    10501 tgctgattga gcagtaaagc gcttactgat caccatcccg ccaaaggcaa tcaaccagac
    10561 caccgcggta tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt taatggtgcc
    10621 tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg
    10681 aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag
    10741 agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct gattctactc
    10801 ccccccagta cctgatacta ccccccagta gattaatagt gctatgattt ttcatattct
    10861 tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact gaaagcgcgt
    10921 gccagtaaga ttcagggcca ggtcgtggcg ctcaagaaaa tgctcgacga gccgcacgaa
    10981 tgcgctgcag ttttacaaca gattgctgct atccgtggag cggtaaacgg tctgatgcgg
    11041 gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga gctaaaacgt
    11101 gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata atttattaac
    11161 gcgattgtaa aactgccgtt tttcctcgtt tacaacgggt gcgctggaca ttaccatcct
    11221 cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt gaaggctgag
    11281 aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg accaccaggt
    11341 cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct gagttcagcg
    11401 caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca ctgcatgacc
    11461 aatgatgtgg tgcaaacctt taccgccaat accttgctgg cgctcggtgc atcgccagcg
    11521 atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc cttgttgatt
    11581 aacgttggca cactgacgca gccacgcgca caggcgatgc gtgctgccgt tgagcaagca
    11641 aaaagctctc aaacacgctg gacgcttgat ccagtagcgg ttggtgcgct cgattatcgc
    11701 cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg taatgcttcg
    11761 gaaatcatgg cattagctgg cattgctaat ggcggacggg gagtggatac cactgacgcc
    11821 gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc aatcgtcgtg
    11881 gtcactggcg aggtggatta tgttaccgat ggacatcgta tcattggtat tcacggtggc
    11941 gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt tgtcgctgcc
    12001 tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca ctggatgaaa
    12061 caagccggag aacgcgcagt cgccagaagc gaggggccag gcagttttgt tccacatttc
    12121 cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt aacgctctga
    12181 cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt aaaaccttct
    12241 cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa aatacccgtg
    12301 gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc gattcggtgt
    12361 tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc gatattgttg
    12421 aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc gacaccgtta
    12481 tgctggcaaa aagcggcgac ccgctgcttt caccttcggc ggttgctacg ctgcgcagtc
    12541 gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagctgcc gccttgctcg
    12601 acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg ctgttggcga
    12661 tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa agcccggact
    12721 ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg accaaaaaca
    12781 cccacggcac tggttgtaca ctctctgcgg cgttggctgc actacgcccg cgccatacaa
    12841 actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta gcccaggccg
    12901 acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc tggtggtgag
    12961 caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc agggatgaaa
    13021 ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg cgctcttggg
    13081 ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta ttcgacagca
    13141 gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc tttcgctatt
    13201 cgcattcctg ccagttacac cattcacgga ttagatgttt cgcgctggca ggagcgtatc
    13261 gactggcagc gggtggcaaa aatgcgcgac aacgatatcc gcttacagtt tgcttttatt
    13321 aaggctacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg gcaattaagc
    13381 cgcgaaaatg gcctgctgcg cggggcgtat cattatttct ccccgtcggt atccgcttca
    13441 gttcaggcgc gattatttct gcaaacggtg gatttctcac aaggcgattt ccctgccgtg
    13501 ctggacgtag aagaacgggg aaaattatcg gcaaaagaat tacgcaagcg ggtaagtcag
    13561 tggctaaaaa tggtcgaaaa acgcacggga aaaaagccga ttatttactc aggagccgtt
    13621 ttttatcaca ccaatctggc gggctatttc aatgaatatc cgtggtgggt ggctcactat
    13681 tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca ttccgaccgt
    13741 ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg cacggtggat
    13801 gagttgcagg cattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata
    13861 ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt
    13921 gcccaactga acgagcgtat tttagcggcg gataacacgc cgctttatat taagtttgcc
    13981 gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat tttgcccggt
    14041 gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag
    14101 gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac
    14161 atcttcgaat actcgttgaa agaagcgaga ggtttttctc agcaggtggt attgcgcggg
    14221 aaaaagcccg atacgctatg ggttaataag cgtgtcgtga aatgtcccga agaagttgcg
    14281 cagcagctgg cggtcgaagc gggaagtgat gtctttttgc ttaagcgtat tcgctatgtc
    14341 gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt
    14401 gatgccatcg gaatttctct ttatgactat ttccgcagcc agcacattta cccacaacgt
    14461 acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta
    14521 gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct
    14581 attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag
    14641 ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc
    14701 attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc
    14761 acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgcccgaat
    14821 gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa
    14881 cggcgcggca aatttctcct gccattgctt gccaagtgtt gttatctctg cagataaagc
    14941 aaaacgttcg gcggcaatct cagcctcttg acgattgagt gacactaaag gtcgacaggc
    15001 catgatccgc gccagtaatg cgtccgggat atcgccaata cgtgggccaa aatcgataaa
    15061 cggcgtcacg tcttgcagct tttccagcca ttccactaac aattcgccgc agggcgaggc
    15121 cagttgataa ccggaaaaat agagcaggtt gccaggcgca acggttaatc gtgccaacca
    15181 ttggcgattc cattgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg
    15241 ctcaaccagt gccagacacc agccgttatc gctttcggcg ttatcgatca ggctgattaa
    15301 cccctctttc gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa
    15361 ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc
    15421 gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata
    15481 agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt
    15541 taattcaggc agcagtgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga
    15601 tatttctcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagctcc
    15661 gcctttaacg caggatcgac agcgttaatg ccatgcaacg cgccgcaaat cgccgtcgcc
    15721 atagcaccta tggtgtctgt gtcgccgcca agattggcgc acaggacggc gcagcggttt
    15781 gggtcggttt gtgccagttc aactagcgca atggcgcacg gaacggactc aatagtgctg
    15841 gtgcctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcatta
    15901 cgcacaattt tcagcgcaat ctccagacgt gccgccagtg aggcgctgaa cgtggtggtg
    15961 cgtttttgct gtgcatgtcg cgcaattgaa ggcaatgaat caacaatcgc tgaccagcgt
    16021 tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga
    16081 tcggatttat gggtcgggct ggacgccagc gccacatcat caataaagga atcaacatca
    16141 cgcgctggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggttacgccg
    16201 ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag
    16261 gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca acgcccagtc gagaatatta
    16321 cgcccaatca gattcggatc gatcttgcct tcacattcca gtaacgcatc cgccagacac
    16381 aggccatcga ggtatcgtcg gtgaattcgg cgcggttaaa ataacaggcc gcgttattct
    16441 cctttggtcc aggcagaaaa cggtcaatcc agccaaagtg tgctttaacg cggctgcgtg
    16501 gccaaagctc ggagggcatc cccatcgcat cccctaacgc ctgcccataa agagcaccga
    16561 gaatacgttc tgttttcatt taacttcccc ttgtgtcaac gcaatatctc gatcatcgac
    16621 cttgatagcc gtaatttcgt tgtcggattc gcgggaaaaa atcatgaaca gcacggcgat
    16681 aatggcaatc atcaccgcgc cgaaagtcca catcccggac cagttgaaag tcagtccgtt
    16741 taccggttcc tgataagcga acatcttttc cgtcatcaca ccgccaagac gatagccgag
    16801 caaactgccg aagccctggc agcagagcgt gatcagcccc tgcgcagcgg tacgcatatg
    16861 cacgggggct tttttatcga catagatgta agcggtaacg tagtaaaaat cgtaacttac
    16921 gccgtgaagc aaaataccga ggaacagtaa cgcgtaggtg aaattgacag gtaatgtaag
    16981 gtacgcgatg acaataccac ataataatac cttttgaata cctttgcggt aaaaacttga
    17041 ccgcgctaac attttttctg atgaatcgag ccaacagaaa acgctgaaaa aacatccaaa
    17101 agatggaaaa actcgcccgt aacgaccagg attatgattg cccgcaaaag gcgggccagg
    17161 acaatccata gccgatatcc aatcggaatt tacgggagca tagtaatgac agatattgcg
    17221 cagttgcttg gcaaagacgc cgacaacctt ttacagcacc gttgtatgac tattccttct
    17281 gaccagcttt atctccccgg acatgactac gtagaccgcg tgatgattga caataatcgc
    17341 ccgccagcgg tgttacgtaa tatgcagacg ttgtacaaca ccgggcgtct ggctggcaca
    17401 ggatatcttt ctattctgcc ggttgaccag ggcgttgagc actctgccgg agcttcattt
    17461 gctgctaacc cgctctactt tgacccgaaa aacattgttg aactggcgat cgaagcgggc
    17521 tgtaactgtg tggcatcaac ttacggcgtg ttggtgtcgg tatcgcggcg ctatgcgcat
    17581 cgcattccat ttctcgtcaa acttaatcac aacgagacgc taagttaccc gaatacctac
    17641 gatcaaacgc tgtatgccag cgtggagcag gccttcaaca tgggcgcggt ggcggttggt
    17701 gcgactatct attttggctc ggaagagtca cgtcgccaga ttgaagaaat ttctgcggcc
    17761 tttgaacgcg cgcacgagct gggcatggtg gcagtgctgt gggcctattt gcgaaactcc
    17821 gcctttaaga aagatggcgt tgattaccat gtttccgccg acctgactgg tcaggcgaac
    17881 catctggcgg caaccatagg tgcagatatc gtcaaacaaa aaatggcgga aaataacggc
    17941 ggctataaag cgattaatta cggttatacc gacgatcgcg tgtacagcaa gttaaccagc
    18001 gaaaacccga ttgatctggt gcgttatcag ttagctaact gctatatggg ccgggctggg
    18061 ttgataaact ccggcggtgc tgcaggcggt gaaactgacc tcagcgatgc agtgcgtact
    18121 gcggttatca acaaacgcgc aggcggaatg gggctgattc ttggacgtaa ggcgttcaag
    18181 aaatcgatgg ctgacggcgt gaaactgatt aatgccgtgc aggacgttta tctcgatagc
    18241 aaaattacta ttgcctgata cgcctcatac gaacagcacc accccgtagg acggataagg
    18301 cgtttacact gcatccggca acctatgcct gatgcgacgc ggaagcgtct tatcatgcct
    18361 acatagcact gccacgtatg tttacaccgc atccggcata aaaacacgcg cactttgcta
    18421 cggcttccct atcgggaggc cgtttttttg cctttcactc ctcgaacaat tttcatattg
    18481 tcgtttttgt gatcgttatc tcgacattta aaagcaaata atttcattat tttttgaaat
    18541 cgaaagcaaa caacaagata tgaaaatgta cgtggtatcg acaaagcaga tgctgaacaa
    18601 cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg aaacgatgca
    18661 agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg gaacgcctgg
    18721 cacatttact catgctggta cagaaaatct gttggcgctg gtcagcgcga tggcgaagca
    18781 atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg atatcgctca
    18841 gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc cttttgcgca
    18901 aaatatttca cgggtcaaag aggtggtgga tttttgccat cgctttgatg tcagcgtcga
    18961 ggcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg aagccgatgc
    19021 gttgtacacc aaccccgcac aggcgcgtga atttgccgag gcaaccggga ttgattccct
    19081 ggcggtcgcc atcggcacgg ctcacggtat gtatgccagc gcaccggtgc ttgatttttc
    19141 tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg gcgcgtcagg
    19201 attatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa tcaacgttgc
    19261 aacggagctg aaaaatgcct tctcgcagtc gttaaaaaat tacctgaccg agcaccccga
    19321 agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg atgtggtgag
    19381 caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc atttaacaag
    19441 gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc ggcatatgtt
    19501 cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat cgcaacagca
    19561 cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc ggttataccg
    19621 gaatgacact ggcagacttt cgcgaatttg tttttacgat tgccgataaa gttgggtttg
    19681 cacgcgaacg cattattctc ggcggcgatc atctggggcc aaactgctgg cagcaagaaa
    19741 atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt cgtgctggct
    19801 tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata ccgttagcac
    19861 cagaaacggt tgcggaacga gctgctgtgc tttgctttgc tttgctgcgg aaagtgtggc
    19921 gacagattgc cagcgtgagc aactgagcta tgtcattggc actgaagttc cggttccggg
    19981 cggtgaggcc agcgccattc agtcagtaca catcacccat gttgaagatg ccgccaatac
    20041 tttacgtacg catcaaaagg cctttattgc ccgtgggctg acagaggcgt taacacgtgt
    20101 gattgccatc gtggtgcagc cgggtgtgga atttgatcac agcaatatta tccattatca
    20161 gccgcaggaa gcgcaggcgc tggcgcaatg gatagaaaac acccgaatgg tttatgaagc
    20221 acattctacc gattaccaga cccggacggc ttattgggaa ttagtccgcg atcactttgc
    20281 aatattgaaa gtcggtcctg cattaacctt tgctttacgc gaggcgatat ttgcactggc
    20341 acaaattgag caggaactta tcgcccccga aaatcgcagc ggttgcctgg cggtaattga
    20401 agaagtgatg ctcgacgaac cgcaatactg gaaaaaatat tatcgcacgg gttttaacga
    20461 ttcattactg gatattcgtt acagcctgtc ggatcgtatt cgttattact ggccgcatag
    20521 tcggattaaa aatagcgttg aaacgatgat ggtgaatctg caaggcgtgg acatcccact
    20581 gggcatgatt agtcagtatc ttcccaaaca atttgaacgc attcagtccg gggaattatc
    20641 agcaatgccg catcagctta ttatgaataa aatttatgat gttttgcgcg cctatcgcta
    20701 cggttgtgcg gaataaggac ggtatatgac taacctgttt gttcgtagcg gaatttcttt
    20761 tgtcgatcgt agcgaagttt taacctatat cggtaatgag atgctcgcga aaggtgtggt
    20821 tcatgatacc tggccacagg cattaattgc cagagaagca gaattcccta ccgggataat
    20881 gcttgagcag cacgccattg caataccgca ttgtgaggcg attcatgcta agtcgtcagc
    20941 catttatctg ttaaggccaa caaataaagt tcattttcag caagcggatg atgataacga
    21001 cgtggcggta tcgttggtta ttgcgttgat tgtggaaaat ccgcagcagc aattgaaact
    21061 tttacgctgt ttatttggca agttacaaca gcccgatatc gtcgagacac taattactct
    21121 tcctgaaact cagttaaagg aatacttcac aaagtatgtt ttagattcag acgaataaat
    21181 ccctctgtaa caataaaaag gactatttat gaaacgcaag attattgtcg cttgcggagg
    21241 cgcggttgcg acctctacga tggcggcgga agaaattaaa gagttgtgtc agagtcataa
    21301 tattcctgtt gaattaatcc agtgtcgggt taatgaaata gaaacctata tggatggtgt
    21361 gcatttgata tgtaccactg ccagggtgga tcgtagtttt ggcgatattc cgttagttca
    21421 cggcatgcct tttgtttctg gtgtcggtat cgaagcatta caaaataaaa ttctgactat
    21481 cttacagggg tgacctatgt tttcagaagt catgcgttat attctcgacc tcggccctac
    21541 agtgatgctg ccggttgtca tcattatttt ttctaaaata ttaggcatga aggcaggcga
    21601 ttgctttaaa gcgggtctgc atatcgggat tggctttgtt ggcattggcc ttgtgattgg
    21661 cttaatgctg gattccattg gtccggcggc gaaagcgatg gcggaaaatt tcgacctgaa
    21721 tctgcatgtg gtcgatgtcg gctggccggg ctcttcacca atgacctgga cgtcgcaaat
    21781 tgcgctggtg gcgattccga ttgcgatatt ggttaacgtg gcgatgttac tgacccgtat
    21841 gacgcgggtg gtaaatgttg atatctggaa tatctggcat atgaccttca ccggcagcgt
    21901 tgctgcacct ggcaaccggt tcatggatga tagggatggc gggtgtggta attcacgcgg
    21961 cgtttgttta taagctcggc gactggtttg cccgcgatac ccgaaatttc tttgagctgg
    22021 aaggtattgc tattccgcac ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg
    22081 atgctatcat cgagaaaatc ccaggcgtta accgaattaa atttagcgcc gacgatattc
    22141 agcgcaaatt tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg gggctgatta
    22201 tcggcatcct cgcgggttac gatgtcaaag gtgtattgca gctggcggta aaaacggcgg
    22261 cagttatgct gctgatgcca cggatgatta aacccatcat ggatggttta acgcccatcg
    22321 ctaagcaggc ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc ctgattggcc
    22381 tggatcccgc attactgctg gggcatacgg cggtggtatc ggcaagcctg atttttattc
    22441 cgctcaccat tttaattgct gtttgtgttc cgggtaatca ggtactgccg tttggcgatc
    22501 ttgccactat cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc
    22561 gcaccttaat ctcgggtgtc atcattatga gcatcaccct gtggatcgcg acgcaaacta
    22621 ttggtttgca cacccaactg gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg
    22681 cttcaatgga tcagggcggt tctcccatta cctggttact gattcaggtt ttctccccgc
    22741 aaaatattcc cggtttcatt attatcggcg caattt
//
LOCUS       DAJTNM010000059        22590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTNM010000059 DAJTNM010000000
VERSION     DAJTNM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.059"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..603
                     /locus_tag="OO172_002334"
     CDS             <1..603
                     /locus_tag="OO172_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5512497.1"
                     /translation="LKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSCLNSALYTASR
                     MLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSG
                     AIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPA
                     QQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            624..1282
                     /gene="csiR"
                     /locus_tag="OO172_002335"
                     /pseudo
     CDS             624..1282
                     /gene="csiR"
                     /locus_tag="OO172_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1283..1732)
                     /gene="kbp"
                     /locus_tag="OO172_002336"
     CDS             complement(1283..1732)
                     /gene="kbp"
                     /locus_tag="OO172_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5512498.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1816..1974)
                     /locus_tag="OO172_002337"
     CDS             complement(1816..1974)
                     /locus_tag="OO172_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5512499.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2157..2456
                     /locus_tag="OO172_002338"
     CDS             2157..2456
                     /locus_tag="OO172_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5512500.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2466..2990
                     /gene="ygaP"
                     /locus_tag="OO172_002339"
     CDS             2466..2990
                     /gene="ygaP"
                     /locus_tag="OO172_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5512501.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3037..3441)
                     /gene="stpA"
                     /locus_tag="OO172_002340"
     CDS             complement(3037..3441)
                     /gene="stpA"
                     /locus_tag="OO172_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5512502.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4109..4546
                     /gene="alaE"
                     /locus_tag="OO172_002341"
     CDS             4109..4546
                     /gene="alaE"
                     /locus_tag="OO172_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5512503.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4583..4933)
                     /locus_tag="OO172_002342"
     CDS             complement(4583..4933)
                     /locus_tag="OO172_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5512504.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
     gene            5085..5414
                     /locus_tag="OO172_002343"
     CDS             5085..5414
                     /locus_tag="OO172_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5512505.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     QLSMRKS"
     gene            5662..5907
                     /gene="nrdH"
                     /locus_tag="OO172_002344"
     CDS             5662..5907
                     /gene="nrdH"
                     /locus_tag="OO172_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5512506.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            5904..6314
                     /gene="nrdI"
                     /locus_tag="OO172_002345"
     CDS             5904..6314
                     /gene="nrdI"
                     /locus_tag="OO172_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5512507.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            6287..8431
                     /gene="nrdE"
                     /locus_tag="OO172_002346"
     CDS             6287..8431
                     /gene="nrdE"
                     /locus_tag="OO172_002346"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5512508.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            8441..9400
                     /gene="nrdF"
                     /locus_tag="OO172_002347"
     CDS             8441..9400
                     /gene="nrdF"
                     /locus_tag="OO172_002347"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5512509.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            9755..10957
                     /gene="proV"
                     /locus_tag="OO172_002348"
     CDS             9755..10957
                     /gene="proV"
                     /locus_tag="OO172_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5512510.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            10950..12014
                     /gene="proW"
                     /locus_tag="OO172_002349"
     CDS             10950..12014
                     /gene="proW"
                     /locus_tag="OO172_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5512511.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            12072..13064
                     /gene="proX"
                     /locus_tag="OO172_002350"
     CDS             12072..13064
                     /gene="proX"
                     /locus_tag="OO172_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5512512.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            13256..14435
                     /locus_tag="OO172_002351"
                     /pseudo
     CDS             13256..14435
                     /locus_tag="OO172_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14559..15296
                     /gene="ygaZ"
                     /locus_tag="OO172_002352"
     CDS             14559..15296
                     /gene="ygaZ"
                     /locus_tag="OO172_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5512513.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            15286..15621
                     /gene="ygaH"
                     /locus_tag="OO172_002353"
     CDS             15286..15621
                     /gene="ygaH"
                     /locus_tag="OO172_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5512514.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            15712..16242
                     /gene="emrR"
                     /locus_tag="OO172_002354"
     CDS             15712..16242
                     /gene="emrR"
                     /locus_tag="OO172_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5512515.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            16369..17541
                     /gene="emrA"
                     /locus_tag="OO172_002355"
                     /pseudo
     CDS             16369..17541
                     /gene="emrA"
                     /locus_tag="OO172_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            17558..19096
                     /gene="emrB"
                     /locus_tag="OO172_002356"
     CDS             17558..19096
                     /gene="emrB"
                     /locus_tag="OO172_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5512516.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(19160..19675)
                     /gene="luxS"
                     /locus_tag="OO172_002357"
     CDS             complement(19160..19675)
                     /gene="luxS"
                     /locus_tag="OO172_002357"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5512517.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(19825..21381)
                     /gene="gshA"
                     /locus_tag="OO172_002358"
     CDS             complement(19825..21381)
                     /gene="gshA"
                     /locus_tag="OO172_002358"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5512518.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(21454..21882)
                     /locus_tag="OO172_002359"
                     /pseudo
     CDS             complement(21454..21882)
                     /locus_tag="OO172_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
     gene            complement(21879..22445)
                     /gene="yqaB"
                     /locus_tag="OO172_002360"
     CDS             complement(21879..22445)
                     /gene="yqaB"
                     /locus_tag="OO172_002360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5512519.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         5436 a         5756 c         5659 g         5739 t
ORIGIN      
        1 ctgaaagccg ttggatctta tcgctcggtt ctggaattgc tcaatattcc ccatgcgaaa
       61 ttaatcatgg actgtgtgat attactttcc gtaaccagtt gcctgaactc ggccctgtat
      121 accgcctcaa ggatgctcta ctccttaagc cgtcgcggtg atgctcccgc ggtaatgggt
      181 aaaatcaacc gcagtaaaac cccgtacgtg gcggtgttac tctccaccgg cgcagcattc
      241 ttaacggtgg tggtgaacta ttacgcgcct gcgaaggtat ttaaatttct gatcgacagc
      301 tccggcgcta tcgccctgct ggtttattta gtcatcgccg tttcacagtt gcggatgcgc
      361 aaaattctgc gagcagaagg aagcgaaatt cgcttgcgaa tgtggcttta tccctggctc
      421 acctggctgg tcatcggctt tattaccttt gtgttggtag tgatgctatt ccgtccggcg
      481 caacagttag aagtgatctc caccggctta ttagcgatag ggattatctg taccgtgcca
      541 attatggctc gctggaaaaa gctggtattg tggcaaaaaa cacccgttca taatacgcgc
      601 tgataatgat caggagtcac accatgacca ttacgtctct ggatggctat cgctggctga
      661 agaacgatat tattcgcggt aattttcaac cggatgaaaa attacggatg agtttactga
      721 catcgcgtta tgcacttggc gttgggccgt tgcgggaagc actttctcaa ctggtggcgg
      781 aacggctggt cacggtggtg aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg
      841 agctgctctg acatagacgc cacccgatat ggaagcgatg ctggtcagtc tggcgattgc
      901 ccgcggtggc gatgaatggg aggcagacgt tctcgcaaaa gcgcatctgc tcaataagct
      961 ggaagcctgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc atcaggcgtt
     1021 tcatacagca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc gtgaacggtt
     1081 gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg cgaacggtgc tttcggtgga
     1141 aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac tggcgcgaga
     1201 tacagcgcgc gtcagtgagt taatgcgcca gcatttactg acgccaattc ccattatcca
     1261 gcaggcgatg gctggcaatt aactactctt ccggaatacg caacacttgc cccggataaa
     1321 ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg tacagattag
     1381 cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta acggtataaa
     1441 actggctggc agtggctggt gtcgccgttt tcacctgatc atcaacactg gcaataccgg
     1501 aaatattccc caccgcaaca aggattttct ccttcgcctc ctgactcagg ccgtcaccag
     1561 tgaccgtcgc tttgccgtcg gcaatttgaa tgttcacttt atcggcatca ggtataccgg
     1621 ttttgctcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc tgacctgtaa
     1681 ccgcatccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc atagctacct
     1741 cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt taacgccgcg
     1801 ggggtgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca aaccggggat
     1861 atacccaagc agtgtcagca gaatattaat aatgaacgcc caaccgaacc ctttaccgag
     1921 cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac ccatatgtac
     1981 tccctataag aaaattactc attgtttaaa aagagatttt atctcttaag cgtagtaatt
     2041 ttagcggagc ctgccagttt cagcttacgc ttttactctc atttacgctg ctgaggctgg
     2101 tatatgttgc atttttagtt tagagtttac ttaatttaga aaatacttaa ataaatatga
     2161 ctgaactcgc gcaattacag gccagtaccg aacaggcagc ggccttattg aaagcaatga
     2221 gccaccctaa acggttgctg attctgtgca tgcttagcgg ttcccccggc accagcgcgg
     2281 gagagctgac gcgcattacc ggactgagcg cctctgcgac atcacagcat ctcgctcgta
     2341 tgcgggacga agggcttatc aacagccaac gggatgccca acgcattcta tattccatta
     2401 aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag
     2461 tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc gcacgcggcg
     2521 caaagttaat cgatattcgt gacgctgatg aatatcttcg tgaacatatt cctgaagcag
     2581 atctggctcc attatccgtg ctggaacagt caggtcttcc acctaaatta cgtcgcgagc
     2641 aaattatatt ccactgccag gcaggtaaac gcaccagtaa taacgccgat aaattagcag
     2701 cgattgccgc ccccgcagaa atatttttac tcgaagatgg gattgatggc tggaaaagag
     2761 ctggattgcc agtagcggta aataaatctc aacccttgcc gttaatgcgc caggtgcaga
     2821 tcgctgcggg tggtttaata ttaatcggcg ttgtactggg ttataccgta aatagcggtt
     2881 tcttcttatt aagtggcttt gttggtgccg ggttactgtt tgcaggaatc agcggttttt
     2941 gtggaatggc aaggttgtta gataagatac cgtggaacca acgagcttga gaagcgacgc
     3001 cggacgcgcc ctagcagcga catccggcct cagtaattag atcaggaaat cgtcgagaga
     3061 tttaccttct gccagcgcct gagcaattgg cttcggtgta cggccctgac cggtccaggt
     3121 tttagtttca ccgttaacat cagtgaattt atatttcgcc ggacgcggct ggcgtttttt
     3181 accagcacgt ggtgcagcag cagagctatt acccaataac tcttccgggt taattccgtc
     3241 agctttcatc agctccagcc aggtgctaat tttttcctga cgctcagcca gttcacgctg
     3301 ctgctgttct tcttcttcac gtctttcttt agtgacaacc ctgaattttt cgagcatttc
     3361 ttcaagaacg tcaatggaga attcgcgagc catcgcacgg agggtgcgaa tgttatttaa
     3421 actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca aaaaagtatt
     3481 tcgtcgcgta gtgtaagcga tcacttatta tatttccaca aaatttaatc tatttacagt
     3541 cctgcgcgag attatttcag ctgttatttt ctgaaactct cagaataatt tcaagattat
     3601 atattagcca ttttgatgag tattttaggt tattggtgaa cagagtggcg ctctctgctc
     3661 gctaactccg taagcctgac aaaaccgaag tgaataaaaa tgcaatataa cagattaaaa
     3721 gaaaacaccc ctcactggtg ctttctgctg ttaatgtaca ttttctggca tcaaaaatgc
     3781 tgtaaatgac ccggtcacga caaacaccga attctctacg cttatagcgt tacctccccc
     3841 ccaaactttc attagtgaaa ttaaaggcaa ggccatatcc aattgattta attgaaaata
     3901 aatcatatcg gcatgtaaaa atccacaatg tacaaaaaac gaaccgttac ggaatatttt
     3961 atctacaaaa gctgactaaa taaaaatttt tcactaattt attagtcata gccagcgata
     4021 tacgctatgc gaaaatacag atggcaatga gatccactgc tttcatctcc attaacatcc
     4081 cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct tgcgtcatgc
     4141 agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt gtattgaagt
     4201 tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag cgattccggt
     4261 gaacatctta attgcatggc catacggtat gtaccgcgat ctgtttatgc gcgcggcacg
     4321 caaaggctgg ataaaaaatc tggctgatat cctggcttat gtgacgttcc agtcaccggt
     4381 gtatgtggcg atcttgttag tggtgggcgc agactggcat cagattatgg cggcggtcag
     4441 ttcaaacatc gttgtttcga tgttgatggg ggcggtttat ggctacttcc tcgattattg
     4501 ccgccgactg tttaaagtca gccgttacca gcaggtaaaa gcctgactct tcttttcgcg
     4561 actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc aaataacgtt
     4621 caagcgcaac acgcgaacta aagccatgcg ggataccgta atgctcgtgt cgctgtcgct
     4681 cgcctgctaa atagagcgga aattgctggt aatgatcgca agttttaata tcggaagcgg
     4741 gttcagccag tgttgaactg cgttctttta atgttggatg aattaccaga gcggtacgtc
     4801 ccattcgcgc ttcgcgatta acatagacat aattttcccc acggcggtaa ccatacgctt
     4861 tctgggttac cacatcgaca gtaaaaccga ctttttcaag tacgcgcgcc acctcgtctg
     4921 gtcgtaaata catatttgat cctcgttatt ttcaaccgcg ggcctacctt acctgattgc
     4981 gcattcacaa cgctttcaga aaagtccata aagccgcgat gaacctcagt taagaaaatt
     5041 atggtctaca ctgaaaatta catcgaattc taatggggga tcatatgttt aaccgaccga
     5101 accgcaacga cgttgatgat ggcgtgcagg atatccagaa tgatgtcaat caattagctg
     5161 acagcctcga atctgtattg aagtcctggg gcagcgacgc caaaggggaa gctgaagccg
     5221 cacgcagtaa agctcaggcg ttgctgaaag aaaccagggc gcgaatgcat ggtcgtactc
     5281 gcgtccagca agccgcgcgc gatgccgttg gctgcgcgga ttcttttgtt cgtgaaagac
     5341 cctggtgtag cgtgggtaca gcagctgcgg taggtatttt tatcggcgca cagttaagca
     5401 tgcgcaaatc gtagtgcaaa aatgataata aatacgcgtc tttgaccccg aagcctgtct
     5461 tcggggtttc tttttgcctg gtgaatcaca aaaatccccc tcccccgtca cgctcatatc
     5521 cagggtaatt tcgatcacta tttgctatat attgtgtggt tgaatctttt ttcaactaca
     5581 tctagtatct ctgtatcaac agagagacaa cccgacgcgt atcatcgcgc cgtatcttca
     5641 ttttaaacag aaatacgaat catgcgcata actatttata ctcgtaacga ttgcgttcag
     5701 tgccacgcca ccaaacgggc gatggaaaac cggggctttg attttgaaat gattaatgtc
     5761 gatcgcgttc ctgaagcagc ggaagcgttg cgtgctcagg gctttcgtca gttgccggta
     5821 gtgattgctg gcgatcttag ctggtctggt ttccgtccgg acatgattaa ccgtctgcat
     5881 ccagcgccac acgcggccag tgcatgagcc agctcgtcta cttctccagc agctccgaaa
     5941 acacgcagcg ttttatcgaa cgtttaggtc tgcccgcggt gcgcatcccg ctcaatgagc
     6001 gggaacggat tcaggtagac gagccttaca tcctgatcgt gccctcttac ggcggcggcg
     6061 gtacggctgg cgcggtgcca cgacaggtaa ttcgcttttt aaacgacgag cacaaccggg
     6121 cgttgcttcg cggcgttatt gcttctggta atcgcaactt tggtgaggcg tatggccgcg
     6181 ccggagatgt gattgcccgg aaatgcggcg tgccgtggct gtaccgtttt gaactcatgg
     6241 gtacgcaaag cgatatcgaa aacgttcgta aaggagtaac cgaattttgg caacgacaac
     6301 cgcagaatgc ctgacgcagg aaacgatgga ttaccacgcg ctgaatgcga tgcttaatct
     6361 ttacgatagc gcaggtcgca ttcagttcga taaagaccgc caggccgttg acgcctttat
     6421 tgcgacgcat gtgcgtccga acagtgtgac cttcagtagc cagcagcagc gcctgaactg
     6481 gctggtcaac gaaggttact atgatgaaag cgttcttaat cgctactctc gcgactttgt
     6541 cattacgctg tttgcccacg cacacaccag cggttttcgt ttccagacat tcctcggggc
     6601 atggaagttt tacaccagct atacgttgaa gacattcgac ggtaaacgtt atctggaaga
     6661 ttttgccgat cgagtcacga tggtggcgct gacgctggca caaggcgatg agacgctggc
     6721 gttgcaactg accgatgaaa tgctgtcagg acgctttcag ccagccacgc caacattcct
     6781 caactgcggt aagcagcagc gcggcgaact ggtttcctgt tttttgctgc gtattgaaga
     6841 caatatggag tcgattggtc gggcggtaaa ttccgcactg cagctgtcga aacgcggcgg
     6901 cggcgtagca tttttgctgt cgaatctgcg ggaagcgggc gcgccaatta aacgtattga
     6961 aaatcaatct tctggcgtaa ttccggtgat gaaaatgttg gaagacgcat tttcctatgc
     7021 caaccaactc ggcgcgcgtc agggggcagg tgcagtctat ttacatgctc atcatcccga
     7081 tattctgcgt tttctcgaca cgaaacggga aaatgccgac gaaaaaatcc gcattaaaac
     7141 actgtcgctg ggcgtggtga tcccggatat cactttccat ctggcaaaag agaatgcgca
     7201 gatggcgctg ttttcgccct atgacgttga gcgagtttat ggcaagccgt ttgccgatat
     7261 cgccattagc gaacactatg acgaactggt tgccgatgaa cgcatccgca aaaaatacct
     7321 caacgcccgt gatttcttcc agcgactggc agaaatccag tttgagtccg gctatcccta
     7381 catcatgtat gaagacacag taaaccgtgc taaccctatc gccgggcgca taaatatgag
     7441 taatctctgc tcagaaattt tgcaggttaa cagcgcctca gagtatgacg agaatctcga
     7501 ctatgcccgc acaggccatg atatttcctg caatttaggt tcgttgaata ttgcgcacac
     7561 catggattcc cccgattttt cccgcacggt agagactgcc gtgcgcggtt taacggcggt
     7621 gtcagatatg agtcatatcc gcagcgtgcc gtccatcgaa gccggaaatg ccgcctcgca
     7681 cgccatcgga ctggggcaga tgaatttaca tggttatctg gcgagggaag gcatcgctta
     7741 tggttcgccg gaagccctgg atttcaccaa tctctatttc tataccatca cctggcacgc
     7801 actgcgtacc tcgatgttgc tggcgcgcga acgcggtgaa accttcgccg ggttcaaaca
     7861 gtcacgctat gccagtggtg aatatttcag ccaatatctg caaggtaact ggcagccgaa
     7921 aacggcgaaa gttggcgaac tgtttgcccg cagcggtatt acgttaccta cccgtgagat
     7981 gtgggcgcaa ctgcgcgacg acgtgatgcg ctacggcata tacaaccaga atctccaggc
     8041 ggtgccgcca accgggtcta tctcttatat caaccatgct acgtcgagta ttcatccgat
     8101 tgtggcgaaa gtagagatac gcaaagaggg caaaacagga cgcgtttact accctgcccc
     8161 gtttatgact aacgaaaatc tggcgctgta tcaggacgct tacgaaattg gcgcagaaaa
     8221 gatcatcgac acctacgcgg aagcgactcg ccatgtcgat caggggctgt cgctgacgct
     8281 gtttttcccc gataccgcca ccactcgcga tatcaacaaa gcgcagattt acgcctggcg
     8341 caaaggtatc aaaacgctct attacatccg cctgcgtcag atggcgctgg aaggcactga
     8401 aattgaaggc tgcgtctcct gtgcacttta aggaatatct atgaaactct cacgtatcag
     8461 cgccatcaac tggaacaaga tatctgacga taaagatctg gaggtgtgga atcgcctgac
     8521 cagcaatttc tggctaccag aaaaggtgcc gctgtcgaac gatattcccg cctggcagac
     8581 attaactgtc gtagaacaac aactgacgat gcgcgttttt actggcctga cgctgctcga
     8641 cacgctgcaa aatgttatcg gcgcgccttc gctgatgccc gatgcgctca cgcctcatga
     8701 agaggcggta ttatcgaata tcagctttat ggaagcggtt catgcccgct cttacagttc
     8761 gattttctcg acgctgtgcc agaccaaaga tgtcgatgcc gcctacgcct ggagtgaaga
     8821 aaacgcaccg ttgcagcgaa aagctcagat tattcagcaa cattatcgcg gtgatgatcc
     8881 gctgaaaaag aaaatcgcca gtgtgtttct tgaatctttt ttgttctatt ccggtttctg
     8941 gctgccgatg tatttttcca gccgcggaaa gctgaccaat accgcggacc tgatccgtct
     9001 gattatccgc gatgaagcag tccacggtta ctacataggc tataaatatc agaaaaacat
     9061 ggaaaagata tctctgggac aacgtgaaga gttgaagagt ttcgccttcg atttgttgct
     9121 ggaactctac gacaacgagt tgcaatacac cgatgagctg tacgccgaaa ccccgtgggc
     9181 tgacgatgtg aaagcgtttc tctgttacaa caccaataag gctttgatga atctgggcta
     9241 cgaaccgtta tttcccgcag aaatggcgga agtgaatccg gcaatcctcg ccgcgctttc
     9301 gccgaatgcc gatgaaaatc acgatttctt ttccggttca ggctcctctt atgtgatggg
     9361 gaaagcggtt gaaacagaag atgaagactg gaatttctga gggtgttatt ttcaaaaata
     9421 tcactacccg cagcagggaa ataattcccg ccaaatagct ttttatcacg caaatattct
     9481 gtagtgatct acactgatac tctgttgcat tattcgcctg aaactacaat attcaggcgt
     9541 tttttcgcta tctatgacaa aaaatatcaa cttttctcga tttgctctca gcccttatat
     9601 cacggaaaat tccggcgatt tgctcgcatc aatattcatg ccacatttgc cctcaggggt
     9661 tgcctcagat tctcagtatg ttagggtaga aaaaagtgac tattcccatt gggtaatata
     9721 tcgacataaa caaataaagg aatcattcta ttgcatggcc attaaattag aaattaaaaa
     9781 tctttataaa atatttggtg agcatccaca gcgagcgttc aaatatatcg aacaaggact
     9841 ttcaaaagaa caaattctgg aaaaaactgg gctatcgctt ggcgtaaaag acgccagtct
     9901 ggccattgaa gaaggcgaga tatttgtcat catgggatta tccggctcgg gtaaatccac
     9961 aatggtacgc cttctcaatc gcctgattga acccacccgc gggcaagtgc tgattgatgg
    10021 tgtggatatt gccaaaatat ccgacgccga actccgtgag gtgcgcagaa aaaagattgc
    10081 gatggtcttc cagtcctttg ccttaatgcc gcatatgacc gtgctggaca atactgcgtt
    10141 cggtatggaa ttggccggaa ttaatgccga agaacgccgg gaaaaagccc ttgatgcact
    10201 gcgtcaggtc gggctggaaa attatgccca cagctacccg gatgaactct ctggcgggat
    10261 gcgtcaacgt gtgggattag cccgcgcgtt agcgattaat ccggatatat tattaatgga
    10321 cgaagccttc tcggcgctcg atccattaat tcgcaccgag atgcaggatg agctggtaaa
    10381 attacaggcg aaacatcagc gcaccattgt ctttatttcc cacgatcttg atgaagccat
    10441 gcgtattggc gaccgaattg ccattatgca aaatggtgaa gtggtacagg tcggcacacc
    10501 ggatgaaatt ctcaataatc cggcgaatga ttatgtccgt accttcttcc gtggcgttga
    10561 tattagtcag gtgttcagtg cgaaagatat tgcccgccgg acaccgaatg gcttaattcg
    10621 taaaactcct ggcttcggcc cacgttcggc actgaaatta ttgcaggatg aagatcgcga
    10681 atatggctac gttatcgaac gcggtaataa gtttgtcggc gcagtctcca tcgattcgct
    10741 taaaaccgcg ttaacgcagc agcaaggtct tgatgcggcg ctgattgatg cgccgttagc
    10801 agtcgatgca caaacgcctc ttagcgagtt gctctctcat gtcggacagg caccctgtgc
    10861 ggtgcccgtg gtcgacgagg accaacagta tgtcggcatc atttcgaaag gaatgctgct
    10921 gcgcgcttta gatcgtgagg gggtaaataa tggctgatca aaataatccg tgggatacca
    10981 cgccagcggc ggacagtgcc gcgcaatccg cagacgcctg ggggacaccg acgactgcac
    11041 cggctgacgg cggtggtgct gactggctga ccagtacgcc tgcgccaaac gtcgagcatt
    11101 ttaatattct cgatccgttc cataaaacgc tgatcccgct cgacagttgg gtcactgaag
    11161 ggatcgactg ggttgttacc catttccgtc ccgtcttcca gggcgtgcgc gttccggttg
    11221 attatatcct caacggtttc cagcaattgc tgctgggtat gccagcgcca gtggcgatta
    11281 tcgttttcgc tctcatcgcc tggcagattt ccggggtcgg aatgggcgtg gcgacgctgg
    11341 tctcgctgat tgccatcggc gcaatcggtg cctgttcgca ggcaatggtg actctggcgc
    11401 tggtgttaac tgccctgctg ttctgtatcg tcatcggttt gccgttgggg atatggctgg
    11461 cgagaagtcc gcgagcggcg aaaattattc gtccactgct tgatgccatg cagaccacgc
    11521 cagcgtttgt ttatctggtg ccaatcgtca tgctatttgg tatcggtaac gtgccgggcg
    11581 tggtggtgac gatcatcttt gcgctgccgc cgattatccg tctgaccatt ctggggatta
    11641 accaggttcc ggcggatctg attgaagcct cgcgctcatt cggtgccagc ccgcgccaga
    11701 tgctgttcaa agttcagtta ccgctggcga tgccgaccat tatggcgggc gttaaccaga
    11761 cgctgatgct ggccctttct atggtggtca ttgcctcgat gattgccgtc ggcgggctgg
    11821 gtcagatggt acttcgcggt atcggtcgtc tggatatggg gcttgccacc gttggcggcg
    11881 tcgggattgt gatcctcgcc attatcctcg atcgcctgac gcaggccgtt gggcgcgact
    11941 cacgcagtcg cggcaaccgt cgctggtaca ccactggccc tgttggtctg ctgacccgcc
    12001 cattcattaa gtaactctgc acttgcccgg tgacgccggg cattatcacc ctgccaaaaa
    12061 aaggaataac aatgcgacat agcgtacttt ttgcgacagc gtttgccacg cttatctcta
    12121 cacaaacttt tgctgccgat ctgccgggca aaggcattac tgttaatcca gttcagagca
    12181 ccatcactga agaaaccttc cagacgctgc tggtcagtcg tgcgctggag aaattaggtt
    12241 ataccgtcaa caaacccagc gaagtggatt acaacgttgg ctacacctcg cttgcttccg
    12301 gcgatgcaac cttcaccgcc gtgaactgga cgccactcca tgacaacatg tacgaagctg
    12361 ccggtggcga taagaaattt tatcgtgaag gggtatttgt taacggcgca gcacagggtt
    12421 acctgatcga taagaaaacc gccgaccaat acaaaatcac caacatcgca caactgaaag
    12481 atccgaagat cgccaaactg ttcgatacca acggcgacgg taaagcggat ttaaccggtt
    12541 gtaaccctgg ctggggctgc gaaggtgcga tcaaccacca gcttgccgcg tatgaactga
    12601 ccaacaccgt gacgcataat caggggaact acgcagcgat gatggccgac accatcagtc
    12661 gctacaaaga gggcaaaccg gtgttttatt acacctggac gccgtactgg gtgagtaatg
    12721 agctgaagcc agggaaagat gtggtctggt tgcaggtgcc gttctccgca ctgccgggcg
    12781 ataaaaatgc cgataccaaa ctgccgaatg gcgcgaatta cggcttcccg gtcagcacca
    12841 tgcatatcgt tgccaacaaa gcctggaccg agaaaaatcc ggcggcagcc aaactgtttg
    12901 ccattatgca gttgccagtg gccgatatta acgcccagaa cgccattatg catgacggca
    12961 aagcctcaga aggcgatatt cagggccacg ttgatggctg gatcaaagcc caccagcagc
    13021 agttcgatgg ctgggtgaat gaggcgctgg cagcgcagaa gtaattttta ttcgggcgga
    13081 taaggcgttt tcgcctcatc cgccgctctg tacacaatgc ctgatgcgac gctgacgcgt
    13141 cctatcatgc ctacaaacca catcgcacat ttcaacaagc tatctttcat cccatattca
    13201 tcaacatccg ctattattga tttccagctt aatcatcacc tgatgaacaa aaataatgac
    13261 taaacctaat catgagctta gcccggcgct gatcgtgctg atgtctatcg ccaccggtct
    13321 ggcggtagcc agtaactatt acgcccagcc attgctcgac accatcgcgc gtaacttttc
    13381 cctttccgcc agttcggcag gctttattgt taccgccgcg cagttgggct atgccgcagg
    13441 tctactgttt cttgttcccc tcggtgatat gtttgaacgc cgccgcctga ttgtctcgat
    13501 gaccttactg gcagcgggcg gtatgttaat cactgccagc agtcagtcac tggcgatgat
    13561 gatcctcggt acggcattaa ccggtttatt ctcagtcgtg gcacaaattc tggttccgct
    13621 ggcagcgacg ctggcttcac cggataaacg cggcaaagtg gttggcacca ttatgagcgg
    13681 tctgctgttg gggatattgc tggcacgaac cgttgccggg ttactggcaa acctcggcgg
    13741 ctggcgcacc gtcttttggg ttgcttcggt gttaatggca ctgatggcgc tggcattatg
    13801 gcgtggtctg ccacaaatga aatcagaaac ccacctcaac tacccacagt tgttgggttc
    13861 cgttttcagc atgttcatca gcgataagat cctgcgcacc cgcgcgttgc tgggctgcat
    13921 gacctttgcc aacttcagca ttctctggac ctcaatggcc tttttgcttg ccgctccacc
    13981 ttttaactac agcgatggtg acattggtct gtttgggctt gcgggagctg ccggagcgtt
    14041 gggcgctcgt ccggcgggcg gttttgccga caagggcaaa tcgcaccaca actttcggtc
    14101 tgcttctgct gttactttca tggctggcaa tctggtttgg tcacacttcc gtactggcgt
    14161 tgattatcgg cattctggtg ctggacctca ccgtacaggg cgtgcatatc actaaccaga
    14221 cggtaattta tcgaatacat cctgatgcgc gtaatcgcct gaccgcaggt tacatgacca
    14281 gctactttat tggcggtgcc gccggttcgc taatttcagc ctcagcctgg caacatggcg
    14341 gttgggctgg cgtttgtctg gctggcgcga cgattgccct ggttaactta ctggtctggt
    14401 ggcgaggttt tcatcgtcag gaagccgcaa attaagcaat cagcaaccgt tttcgtggct
    14461 ttacacttat aagggtgtta agaagcccat cagtctgata aggttaagat attcattcag
    14521 tctatttata atattaacaa tcgttaagcg tacactctat ggaaagccct actccacagc
    14581 ctgctcctgg ttcggcgacc ttcatggaag gatgcaaaga cagtttaccg attgttatta
    14641 gttatattcc ggtggccttt gcgttcggtc tgaatgcgac ccgtctggga ttctctcctc
    14701 tcgaaagcgt ttttttctcc tgcatcattt atgcaggcgc gagccagttc gtcattaccg
    14761 cgatgctggc agccgggagt agtttgtggg ttgctgcact gaccgtcatg gcaatggatg
    14821 ttcgccatgt tttgtatggc ccgtcactgc gtagccgtat tattcagcgt ctgcaaaaat
    14881 cgaaaactgc cctatgggcg tttggcctga cggatgaggt ttttgccgcc gccaccgcaa
    14941 aactggtacg caataatcgc cgctggagcg agaactggat gatcggcatt gccttcagtt
    15001 catggtcatc gtgggtattt ggtacggtaa taggggcatt ctccggcagc ggcttgctgc
    15061 aaggttatcc cgccgttgaa gctgcattag gttttatgct tccggcactc tttatgagtt
    15121 tcctgctcgc ctctttccag cgcaaacaat ctctttgcgt taccgcagct ttagttggtg
    15181 cccttgcagg cgtaacgcta ttttctattc ccgtcgccat tctggcaggc attgtctgtg
    15241 gctgcctcac tgcgttaatc caggcattct ggcaaggagc gcccgatgag ctatgaggtt
    15301 ctgctgcttg ggttactggt tggcgcggcg aattattgct tccgctattt gccgctgcgc
    15361 ctgcgtgtgg gtaatgcccg cccaaccaaa cgtggcgcgg taggtatttt gctcgacacc
    15421 attggcatcg cctcgatatg cgctctgctg gttgtctcta ccgcaccaga agtgatgcac
    15481 gatacacgcc gtttcgtgcc cacgctggtc ggcttcgcgg tactgggtgc cagtttctat
    15541 aaaacacgca gcattatcat cccaacactg cttagtgcgc tggcctatgg gctcgcctgg
    15601 aaagtgatgg cgattatata acccacaaga atcatttttc taaaacaata catttacttt
    15661 atttgtcact gtcgttacta tatcggctga aattaatgag gtcataccca aatggatagt
    15721 tcgtttacgc ccattgaaca aatgctaaaa tttcgcgcca gccgccacga agattttcct
    15781 tatcaggaga tccttctgac tcgtctttgc atgcacatgc aaagcaagct gctggagaac
    15841 cgcaataaaa tgctgaaggc tcaggggatt aacgagacgt tgtttatggc gttgattacg
    15901 ctggagtctc aggaaaacca cagtattcag ccttctgaat taagttgtgc tcttggatca
    15961 tcccgtacca acgcgacgcg tattgccgat gaactggaaa aacgcggttg gatcgaacgt
    16021 cgtgaaagcg ataacgatcg ccgctgcctg catctgcaat taacggaaaa aggtcacgag
    16081 tttttgcgcg aggttttacc accgcagcat aactgcctgc atcaactctg gtccgcgctc
    16141 agcacaacag aaaaagatca gctcgagcaa atcacccgca aattgctctc ccgtctcgac
    16201 cagatggaac aagacggtgt ggttctcgaa gcgatgagct aacgcgtcat ctcgctcaaa
    16261 aatccagatt tataaaagaa aaaatgaccg gccagcatcg caacatgctg gcctttttgg
    16321 caagcaggtc ggctcagccg atgagttaag aagatcgtgg agaacaatat gagcgcaaat
    16381 gcggagactc aaaccccgca gcaaccggta aagaagagcg gcaaacgtaa gcgtctgctc
    16441 ctccttctca ccttgctctt tataattatt gccgtagcga tagggattta ttggtttttg
    16501 gtactgcgtc atttcgaaga aaccgatgac gcatacgtgg cagggaatca gattcaaatt
    16561 atgtctcagg tgtctggcag cgtgacgaaa gtctgggccg ataacaccga ttttgtaaaa
    16621 gaaggcgatg ttctggtcac tctcgacccg acagatgctc gccaggcgtt tgaaaaagcc
    16681 aaaactgcac tggcttccag cgttcgccaa acccaccagc tgatgattaa cagcaaacag
    16741 ttgcaggcga atattgaggt gcaaaaaatc gccctcgcga aagcacaaag cgactacaac
    16801 cgccgtgtgc cgctgggcaa tgccaacctg attggtcgcg aagagctgca acacgcccgc
    16861 gacgccgtca ccagtgccca ggcgcaactg gacgtcgcta ttcaacaata caatgccaat
    16921 caggcgatga ttctggggac taaactggaa gatcagcctg ccgtgcaaca ggctgccacc
    16981 gaagtacgta acgcctagct ggcgctggag cgtacccgta ttgtcagtcc gatgaccggt
    17041 tatgtttccc gtcgtgccgt gcaaccgggt gcgcaaatta gcccaacgac gccgctgatg
    17101 gcggtcgttc cagccaccaa tatgtgggtg gatgccaact ttaaagagac gcagattgcc
    17161 aatatgcgca tcggtcagcc ggtcaccatc accaccgata tttacggcga tgatgtgaaa
    17221 tacaccggta aagtggttgg tctggatatg ggcacaggta gcgcgttctc actgcttccg
    17281 gcgcaaaatg cgaccggtaa ctggatcaaa gtcgttcagc gtctacctgt gcgtatcgaa
    17341 ctggaccaga aacagctgga gcaatacccg ctgcgcattg gtttgtccac gctggtgagc
    17401 gtcaatacca ctaaccgtga cggtcaggta ctggcaaata aagtacgttc aacaccggtc
    17461 gcggtaagta ccgcgcgtga aatcagccta gcacctgtca ataaactgat cgacgatatc
    17521 gtaaaagcta acgctggcta atccagaggt gcgtgtgatg caacagcaaa aaccgctgga
    17581 aggcgcgcaa ctggtcatta tgacgattgc gctgtcactg gcgacattca tgcaggtgct
    17641 ggactccacc attgctaacg tggcgatccc cactatcgcc gggaatctgg gctcatcgct
    17701 cagccaagga acgtgggtaa tcacttcttt cggggtggcg aatgccatct cgatcccgct
    17761 taccggctgg ctggcaaagc gcgtcgggga agtgaaactg ttcctttggt ccaccatcgc
    17821 ctttgctatt gcgtcgtggg cgtgtggtgt ctccagcagc ctgaatatgc tgatcttctt
    17881 ccgcgtgatt caggggattg tcgccgggcc gttgatcccg ctttcgcaaa gtctattgct
    17941 gaataactac ccgccagcca aacgctcgat cgcgctggcg ttgtggtcga tgacggtgat
    18001 tgtcgcgcca atttgcggcc cgatcctcgg cggttatatc agcgataatt accactgggg
    18061 ctggatattc ttcatcaacg tgccgattgg cgtggcggtg gtgttgatga cactgcaaac
    18121 tctgcgcgga cgtgaaaccc gcaccgaacg gcggcggatt gatgccgtgg ggctggcact
    18181 gctggttatt ggtatcggca gcctgcagat tatgctcgac cgcggtaaag agctggactg
    18241 tttttcatca caggaaatta tcattcttac cgtggtggcg gtggtggcta tctgcttcct
    18301 gattgtctgg gagctgaccg acgataaccc gatagtcgat ctgtcgttgt ttaagtcgcg
    18361 caacttcacc atcggctgct tgtgtatcag cctcgcgtat atgctctact tcggcgctat
    18421 tgttctgctg ccgcagttgt tgcaggaggt ctacggttac acggcgacct gggcaggttt
    18481 ggcctctgcg ccggtaggga ttattccggt gattctgtcg ccgattatcg gccgcttcgc
    18541 gcataaactg gatatgcggc ggctggtaac cttcagcttt attatgtatg ccgtctgctt
    18601 ctactggcgt gcctatacct ttgaaccagg tatggatttt ggcgcgtcgg cctggccgca
    18661 gtttatccag gggtttgcgg tggcctgctt ctttatgccg ctgaccacca ttacgctgtc
    18721 tggtttgcca ccggaacgac tggcggcggc atcgagcctc tctaacttta cgcgaacgct
    18781 ggcggggtct atcggcacgt cgataaccac gaccatgtgg accaaccgcg agtcgatgca
    18841 ccatgcgcag ttgactgagt cggtaaaccc gttcaacccg aatgcccagg cgatgtacag
    18901 tcaactggaa gggcttggga tgacgcaaca gcaggcatca ggctggattg cccagcagat
    18961 caccaatcag gggctgatta tttccgccaa tgagatcttc tggatgtcag ccgggatatt
    19021 cctcgtactg ctggggctgg tgtggtttgc taaaccgcca tttggcgcag gtggcggcgg
    19081 aggcggtgcg cactaagtac aactaagcca gttcatttga actggctttt ttcaattaat
    19141 tgtgaagata gtttactgac tagatgtgca gttcctgcaa cttctctttc ggcagtgcca
    19201 gttcttcgtt gctgttgatg cgtacgtcac gttccagaat gctacgcgca atatcctgcg
    19261 cttcctgcaa cgagtgcatc tggtaagtgc cacactggta gacgttcagt tccgggatct
    19321 gattctgatc ctgcactttc agcacgtctt ccattgccgc tttccaggca tcagcaacac
    19381 gctgctcatc tggcgtacca atcagactca tataaaaacc ggtgcggcag cccattggcg
    19441 agatatcgat aatctctaca ccattaccgt taagatggtt acgcataaaa ccagcaaaca
    19501 ggtgctccag ggtatggatc cctctttctg gcatcacttc tttgttcggc acgcagaagc
    19561 gcagatcgaa cacggtgatt gcgtcgccat gcggggtgtt cattgttttc gccacccgaa
    19621 ctgcaggcgc ttccatccgg gtatgatcga ctgtgaagct atctaacaac ggcatttagc
    19681 cacctccggt aattttttta aaaattttct gaactctttc ttcccaggcg agtctgagta
    19741 tatgaaagac gcgcatttgt tatcatcatc cctgaattca gagatgaaat tttggccact
    19801 cacgagtggc ctttttcttt tctgtcaggc gtgtttttcc agccacaccg caaacggttc
    19861 ggtatcagcg gtttccattt cctgctgacg gcgttcagat gcctcgcgct cggctacaaa
    19921 atcctcttcg cgcagaattt ccagcggctc ttcacgcagc agattacggt aggcttctgc
    19981 aaacgctttg cctgttccgc caataccagt atcaatcata gaccttaaga tacgggcaga
    20041 gaaagtcaga tcgggattat cgaagcaggc aaccagttca tcacacactt tctgatacgc
    20101 ttcgccgccg ttaatgctat ccagcgtttg cgcgacgcgt ttcagatcgt ggaacagatc
    20161 tttacccacc tgcagtaacg ggaactgcgc ggtttcgcag ccgataccca gcgtcaggcc
    20221 cggtttgcga ccttcaagga tcacccggtt ccagttaacg cgcgtacagg caagttcgct
    20281 actgctcatt tccggtgcat cagccagcgc acaccagacc ataaacaggt cgaggaatcg
    20341 cacctgctgt tcatctacac caatcggcga gaacgggttg atgtccagcg aacgcacttc
    20401 aatatattca atgccgccac gtaacagcgc atcagaaggt gactcgccgc tgcgggtaac
    20461 gcgttttgga cgaatcggcg catacagttc gttttcaatc tgcagcacgt tgctattgat
    20521 ttgcagcctc ttaccgtctt tctcaatacc aatcttcgcg tactcttccg atggcgtttt
    20581 gattgcctgt ttaaggcccg ctacgtactc gtaaagatcg ttgaaggtaa taccaagatt
    20641 gctttgcgat ttattggtat agccgagatc gctcaaacga agagaggtcg catacggcag
    20701 gtaatacata ccgcactcgg ttttctcaaa cggcagcgac gttggttttc cttgcaggaa
    20761 agaagaacaa atcgccggag atgcaccaaa cagataagga atgacccaac cgaaacgata
    20821 gtaattgcgg ataacgcgga aatagcccgc agaaattttc tctttggcat cagcgcccga
    20881 gatatcaccg cacttcgctt gccagaatgc cattggcaaa gagaaattgt agtgcacgcc
    20941 ggaaatggtt tgcatcagcg cgccgtagcg atttttcagc ccttcacgat acagcgtttt
    21001 aaagcgtccg gtgttagaag tgccgtactg tgccagttcg atgtcctgac cttctgcgat
    21061 gtagcatggc atacttaacg gccacatccg ctcatcgccc atattgcgcg ccgtataacg
    21121 atgcagatcg cgcataaagg tcagcatatg ttcaatatca ccatccactg gtgtaatgaa
    21181 ttccagcaat gcttccgcaa aatcggtagt aatccatttg tgcgtcagtg cggaacctaa
    21241 tgcttcagga tgacctgttg ttgccagtgt gccatcagca ttaacacgca aagtttcgcg
    21301 ctccagccca cgctgtatcc cctttaacgc ctgaggatgt ttttccagcc aggccagcgc
    21361 ctgtgatacg tccgggatca aattgacctc ccgcctgtca aaatcgtttt aattagcata
    21421 actgtaatgg tgaccatatg tgcaggccta caattagtgc caccacatca tgccctgaac
    21481 ggtcgctgct gcaactgcaa catagcgtaa cgctttacca aggcacaaaa aaaagattac
    21541 tggcccccag gagatgcgca tccatcccgc taacagacac agtaaatcgc caaccacggg
    21601 catccagctt aataatagcg tgactgcacc atagcgtttc agccagccgg tagctttctc
    21661 ttgccagcgc gatgttttac gcaatggaaa gaaacgccca aggataacgt tagttaaccc
    21721 tccaaggcta ttacccattg ttgctgttaa aactaaaacc tagggatgac tgatcccgga
    21781 aagcaacatt gccaccagca cgacttcgga gttgccgggt aatagtgtag cgctgagaaa
    21841 actactggca aacaacgaga aaagcgataa cgcttcactc acagcaagcg aacatccaca
    21901 gcgtccatgc ctgctgcacg ggccgcctga ataccgaaat cggcatcttc aaagaccaca
    21961 cactgcgtcg gttgcacgcc catacgctgc gcgcacaaca aaaatgtgtc tggcgcgggt
    22021 ttatggtgtt tgacgtgatc agcagcgacg acggcgtcaa aataacggcg taatcccagg
    22081 tgcgccagca atgcctctgc gatggcgctt tcactccccg ttcctacagc cattgggcga
    22141 cgaccatgcc aacttttcac cacttcaaca agaggaagcg gctcgacact atccagcagc
    22201 atacttctta ctgcttctgt tttttcacgc gctaacgcat gcgggtcgag atcggcctga
    22261 ttcagctcaa taattgcctg agcaatacgc caggtgggcg atccattaag cgcaatcatc
    22321 gcctgaatat cgtactgaag accgtagtgc cctaatactt cgcgccacgc tttacggtgc
    22381 gtaggctccg tatccaggat tgtgccatcc atatcaaaaa ttaaacctgc ataacgctcg
    22441 tacatggtct tctcgcaagt cgaaaaatat gacgctactt tagcgtaatt gcttgatttt
    22501 gtcgctgatg gcgaaggaga agaaaattca ggaaatggat aaagtagtta tttagaaatg
    22561 agatattttt gaaggaaatt ttttggagaa
//
LOCUS       DAJTNM010000060        22320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTNM010000060 DAJTNM010000000
VERSION     DAJTNM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.060"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(22..693)
                     /gene="queE"
                     /locus_tag="OO172_002361"
     CDS             complement(22..693)
                     /gene="queE"
                     /locus_tag="OO172_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5512520.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            832..972
                     /locus_tag="OO172_002362"
     CDS             832..972
                     /locus_tag="OO172_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512521.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            986..1858
                     /locus_tag="OO172_002363"
     CDS             986..1858
                     /locus_tag="OO172_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR5512522.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKNETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1918..3216)
                     /gene="eno"
                     /locus_tag="OO172_002364"
     CDS             complement(1918..3216)
                     /gene="eno"
                     /locus_tag="OO172_002364"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5512523.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3304..4941)
                     /gene="pyrG"
                     /locus_tag="OO172_002365"
     CDS             complement(3304..4941)
                     /gene="pyrG"
                     /locus_tag="OO172_002365"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5512524.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5169..5960)
                     /gene="mazG"
                     /locus_tag="OO172_002366"
     CDS             complement(5169..5960)
                     /gene="mazG"
                     /locus_tag="OO172_002366"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5512525.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6056..8290)
                     /gene="relA"
                     /locus_tag="OO172_002367"
     CDS             complement(6056..8290)
                     /gene="relA"
                     /locus_tag="OO172_002367"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HCR5512526.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8338..9639)
                     /gene="rlmD"
                     /locus_tag="OO172_002368"
     CDS             complement(8338..9639)
                     /gene="rlmD"
                     /locus_tag="OO172_002368"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5512527.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9696..12452
                     /gene="barA"
                     /locus_tag="OO172_002369"
     CDS             9696..12452
                     /gene="barA"
                     /locus_tag="OO172_002369"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5512528.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12684..14024)
                     /gene="gudD"
                     /locus_tag="OO172_002370"
     CDS             complement(12684..14024)
                     /gene="gudD"
                     /locus_tag="OO172_002370"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5512529.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14045..15385)
                     /locus_tag="OO172_002371"
     CDS             complement(14045..15385)
                     /locus_tag="OO172_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5512530.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15387..16739)
                     /gene="gudP"
                     /locus_tag="OO172_002372"
     CDS             complement(15387..16739)
                     /gene="gudP"
                     /locus_tag="OO172_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5512531.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17173..17622)
                     /locus_tag="OO172_002373"
     CDS             complement(17173..17622)
                     /locus_tag="OO172_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5512532.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17640..18422)
                     /gene="truC"
                     /locus_tag="OO172_002374"
     CDS             complement(17640..18422)
                     /gene="truC"
                     /locus_tag="OO172_002374"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5512533.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTVHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18422..18751)
                     /locus_tag="OO172_002375"
     CDS             complement(18422..18751)
                     /locus_tag="OO172_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5512534.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19373..19918)
                     /gene="syd"
                     /locus_tag="OO172_002376"
     CDS             complement(19373..19918)
                     /gene="syd"
                     /locus_tag="OO172_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5512535.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRMHLASNLAEFLNQLKPLL"
     gene            19986..20834
                     /gene="queF"
                     /locus_tag="OO172_002377"
     CDS             19986..20834
                     /gene="queF"
                     /locus_tag="OO172_002377"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5512536.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20946..22310
                     /gene="ppnN"
                     /locus_tag="OO172_002378"
                     /gene_synonym="ygdH"
     CDS             20946..22310
                     /gene="ppnN"
                     /locus_tag="OO172_002378"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5512537.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5513 a         5916 c         5292 g         5599 t
ORIGIN      
        1 ataacgctta taaatgtgta atcaggcaat atttaaatat ttatgtgttt gcatcgacaa
       61 acgccaatta cgcgcaatac aggtttcaat gcacaatcgt gtggcatcat ccttttggct
      121 aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca gtagttcatc
      181 cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg ctcgctccag
      241 tgcctgtgac aacacttcat agccgccgcg catgttcagc tttggcgata cggtaaccca
      301 ggtattcggt gtgcagcgta cttcatgagt tccactggtt tcgatctggc agctaaaacc
      361 gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct caccacccgt
      421 aatcaccaca tgccgcgcgg tatatccctg acgaccaatg acagccagca agtcttcact
      481 gctcgccgcc ccccacttat cactctcttt ggtcttcgcc agaatgctga aaagggagac
      541 ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac agccaaccgg
      601 gcatccctgt aaacgaataa aaatggcggg gacgccggta aagtaaccct caccttgcag
      661 ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt ggataattgt
      721 taattattgc agatcctgcc gcaacaatca tgtcttatta acattctgtt acatgcagat
      781 ttaaagaaaa atacgaaaca tatcgttaat aattaaaggg agtaacgtat tatgtcagaa
      841 gaaaataaag aaaatggatt taatcatgtc aaaatattca ccaaaattat atttattttt
      901 tctgtattag tttttaatga taacgaatct aaaattaccg atgccgccgt caatttattt
      961 atccagattt aatgagaaat ttataatgcg ctatttcata ctaatgttca ctttcgtatg
     1021 ttcctttgtt gcagcccaac caactattgt cccacaatta cagcaacaag ttacgggtct
     1081 tacgagtagc ttaaactcac aagaaaagaa agaactgacg cacaagttag aatctatttt
     1141 caataatacc caagtgcaaa ttgctgtatt aattgtttcc acaaccaaaa atgagacaat
     1201 tgaacaatat gctacaagag tttttgacaa ttggcgttta ggagatgcca aacgtaatga
     1261 tgggatactg atcgttgttg cctggtcgga tcgcactgtc cgcatccagg taggttatgg
     1321 gctggaagaa aaggtaaccg atgctctggc tggagatatc atccgtagca acatgatacc
     1381 cgcatttaaa caacaaaaat tagctaaggg attagagtta gctataatcg ctttgaataa
     1441 ccaactcact tcacaacacc aatatccggc taatccttca gaaagtgaat caacgtcttc
     1501 cactgatcat tattactttg ctattttttg ggtatttgca gtgatgttct tccctttctg
     1561 gttttttcat caaggcagta atttttgtcg cgcatgtaaa agtggagtct gtatttcagc
     1621 aatccatctt ttagatttat tcctgttctc cgataaaact ttttccattg ctgtattttc
     1681 cttctttttt actttcacca tatttatggt ctttacctgt ttatgtgtac ttcagaaaag
     1741 agcatctggt agaagttatc attcagacaa cagcggttcc gctggaggct caagtggcgg
     1801 cggatttagc ggcgggggag gttcttctgg cggtggcggc gcatccggcc gctggtaact
     1861 ccaccataaa aaatgccagc ccgaaggctg gcattatcaa atcagataaa gtcagactta
     1921 tgcctggcct ttgatctctt tacgaccgtt gtacggtgct ttttcgccca gagcttcttc
     1981 gatacgaatc agctggttgt atttagcaac acggtcagaa cggctcatag aaccagtttt
     2041 gatctggcct gcagcagtac caacagccag atcagcgatg gtagcatctt cagtttcacc
     2101 agaacggtga gagataactg cagtgtagcc agcatctttc gccatcttga ttgcagccag
     2161 agtttcggtc agagaaccga tctggttgaa tttgatcagg atggagttag cgataccttt
     2221 ttcgatacct tctttcagga tcttggtgtt ggttacgaac aggtcgtcac caaccagctg
     2281 gattttgtcg cccagaactt tggtctggta tgcgaaaccg tcccagtcag attcgtccag
     2341 accgtcttcg atagaaacga tcgggtactg tttggtcagt tcttccagga agtgagtgaa
     2401 ttcttcagag gtgaacgctt tgttgccttc gccagccaga acgtatttac catctttgta
     2461 gaattcagaa gctgcgcagt ccatcgccag agtgatgtct ttgcccagtt cataaccagc
     2521 agctttaaca gcttcagcga taacagccag agcttcagcg ttggaaccca ggttcggcgc
     2581 atagccacct tcgtcaccaa cagcagtgtt catgcctttc gctttcagaa cttttgccag
     2641 gtgatggaaa acttcagaac ccatgcggat ggcttctttc acagttttcg cgccaaccgg
     2701 ctgaatcatg aattcctgga tatcaacgtt gttgtcagcg tgctcaccac cgttgatgat
     2761 gttcatcatc ggaaccggca tggagtattt gcccggagtg ccgttcagtt cagcgatgtg
     2821 ctcgtacagc ggcatacctt tagcagctgc agcagctttg gcgttagcca gagatacagc
     2881 caggattgcg ttcgcgccga atttggattt gttttcggtg ccgtccaggt cgatcatgat
     2941 cttgtcaatg ccagcctgat ctttagcatc tttgccaatc agcgcctgag cgatcgggcc
     3001 gtttaccgca gcaacagctt tggttacgcc tttacccagg aaacgggatt tgtcgccatc
     3061 gcgcagttcc agagcttcac gggaaccagt agaagcacct gacggagcag ctgccatacc
     3121 gacgaaacca ccctccagat gtacttcggc ttcaacagtc gggttaccac gggagtcgat
     3181 gatttcacga ccgatgattt ttacgatttt ggacattagg ttttcctcaa gtcactagtt
     3241 aaactgaaac tccagacaaa caacgcgtac ccaaggtacg cgttgccgtt ctaacttttt
     3301 tacttacttc gcctgacgtt tctggaactc gctggcggct ttcacaaagc ctgcaaacag
     3361 cgggtgacca tcacgtggag tagaagtaaa ctccggatgg aactggcaag ccacgaacca
     3421 cgggtgattc ggaacttcga tgatctcgac caactgatca tccccggaac ggcccacaac
     3481 gcgcagacct gcatcttcaa tctgtttcaa cagcatgttg ttgacttcgt aacggtgacg
     3541 atgacgctca acaattgtcg gcgcattgta tagctggcga accaggctat cgtcaaccaa
     3601 ctggcactgc tgtgcgccga gacgcatggt accgccgaga tcgctcttct cgctacgaac
     3661 ttcaacgttg ccgttttcat cgcgccactc ggtaatcagc gccacaaccg ggtacttaca
     3721 gtctggcaca aattctgtag agttggcgtt ctccatgttg gcaacatggc gagcgtaatc
     3781 aattaacgcc acctgcatac ccaggcaaat gcccagataa ggaatattgt tctcacgcgc
     3841 aaaacgcgcg gtcgtaatca tgccttctac gccacgatag ccgaaaccgc caggtacgag
     3901 gattgcgtcc agacctttaa ggatttcaac gccgcgcgtt tcaacatctt gtgaatcgat
     3961 cagtttgatg ttgacgctga cacgattctt cagcccaccg tgtttcagtg cttcgatcac
     4021 tgatttataa gcatccggca gttcaatgta cttgccgacc ataccgatgg tgacttcact
     4081 taccggattc gcttcttcga agataacctg ttcccattcg gacagatttg cttccgggca
     4141 gtttaagctg aatcgtttac aaatataatc gtccagcccc tgagatttca acaggcccgg
     4201 aattttatag atggaatcga cgtctttcag agaaataacc gctttttccg gaacattaca
     4261 gaacaatgca atcttcgcac gttcgttcgc cggtacagcg cgatctgaac gacaaatcag
     4321 gatgtcaggc tggataccga tggagagcag ctcttttaca gagtgctgag tcggtttggt
     4381 tttgacttca ccagacgctg ccatgtacgg caccagcgtc aggtgcataa acagagtgtg
     4441 ctcacggcca atttcaacag ccatctggcg aatagcttcg aggaacggca aggattcgat
     4501 atcacctact gtaccgccga tttctaccag tactacgtca tgaccttcgc caccttccag
     4561 cacgcgctct ttgattgcgt tagtgatgtg cggaataacc tgcacggttg cgccgaggta
     4621 gtcaccgcgg cgttctttac gcagaacgtc agagtagata cgacccgtgg tgaagttgtt
     4681 gcggcggctc attttggtac gaatgaaacg ctcgtagtgc cccaggtcca agtcggtttc
     4741 agcgccgtct tcagtaacga acacttcccc gtgttggatt gggctcatag tacctggatc
     4801 gacgttgatg taaggatcca gtttcatgat ggtcacattg aggccacggg cttcaagaat
     4861 ggctgcgagg gaggctgcgg caatgccttt acccagagag gatacgaccc cgccggtcac
     4921 aaaaatatag ttcgttgtca tgctgaacct gagaagttag gttgaaaaga cgatggaata
     4981 accaagacgg gaaagcagta tacccgaaca tgacctgtgc cacaaacttt cattatccct
     5041 cctcttcgcc agcgcactat tgaaatcagg agtgagaaaa tagccccttt ggggtaaatg
     5101 tttttgacgc aaatcaagcg cttgtcattt aaaaaatgac acaaatggcg cttgaccgcg
     5161 taattccctt agagatcaat ttcctgccgt tttacctgtt gccagacttc ttccattgtt
     5221 tcgaggtcaa cacctgtcat ttccagtcca cgcgcggcaa caatacgctc cacttcgcga
     5281 aaacgacgct cgaatttttc gttcactttt tgcaatgcga tttctgcttt cgtccctaaa
     5341 tggcgagcca gattaaccgt ggcaaacagc aggtccccca tttcctcctc cagtttagcc
     5401 tggtcgacaa cagcctgccg cgcctcgtac atcacctcgt cgatctcttc gtagacttta
     5461 tcaaccaccg ggccaagcgt cgtccagtcg aagccaacgt tggcgcaacg tttctggatt
     5521 ttttgcgccc gcattaaagc cggtaaacta cgaggaatat cgtccagcgc cgaatgctgc
     5581 gctttctgcg cgcgctcttc ggttttgatt tgctcccaac gggcaagcac ttcactactg
     5641 ttttcggcag aactatcagc aaaaacatgc ggatggcgac gctctaattt atcgctaata
     5701 gcagcgcaaa tatcattaaa gtcaaagcgc ccttcttcct gagccatttg cgcgtaaaac
     5761 accacctgga atagcagatc gcccagttca ccgcgaagat cgtcaaaatc ttcacgggcg
     5821 atggcgtcca gcacttcgta ggtttcttca agggtgtaag gcgcaatggt ggcaaatgtc
     5881 tgctctttat cccacgggca gccgttttcc ggatcgcgca ggcgctgcat aatagtgagc
     5941 aaacggtcga tttgattcat tgaattgtcc tgaaaattgc gggtctgtca ggtgtaatac
     6001 cttcgagtaa aagatatcgc tggatacggc tgacgccata tccagctatg taagactaac
     6061 tcccgtacaa ccgacgcgcg tcgataacat ccggcacctg gttgagttta cccagcacgc
     6121 gccccagcac ttgcaggttg taaatctcaa tggtcatgtc gatggtcgcc agttgctgtt
     6181 tggtgtcgct acggctggca acgccaagca cgttcacctt ctcgttggcg agaatggtcg
     6241 tgatatcacg taacaaccca ctacgatcat tagccaccac gcggaccacc agcgaatatc
     6301 cggcggagta gctctcaccc cataccgcgt caacaatgcg ttctggcgca tgggagcgca
     6361 gttccgccag ttgttcgcaa tcggcgcggt gtactgaaat accgcgtccc tgggtaatga
     6421 agccgacaat ctcatctcca ggaatcggct ggcagcagcg cgcgatgtgg tgcatcaggt
     6481 taccaacacc ttcaaccacc acacgaccgt tatctttact gcggttttgc ggtgtgtagc
     6541 ttttttgctg aagctgtttc agcgcggcgg cgtcctgctc ttcggcactc ggcttattaa
     6601 attgcgattg caggaagttc accatctgat tgagacggat atccccgcca ccaatcgccg
     6661 ccagcaactc gtcgacatca ttgaagttgt aacgcggcag cagatgtttt tctgcttctt
     6721 tcaggctgat ccccagatgt tccagctcgt cgtcaaggat ttgccgccca gccagaatgt
     6781 ttttgtcacg gtcctgttta cggaaccagg cgtgaatttt cgaacgccca cggctggttg
     6841 tgacgtaacc gaggtttggg tttaaccagt cacggctggg gttcggctgt ttctgggtga
     6901 taatttcaat ctggtcgccc atttgcagct ggtaggtgaa cggcacaatg cgcccgccaa
     6961 tttttgcccc gatgcagcgg tgtccgacat cactgtggat gtggtaagcg aagtccagcg
     7021 gcgttgatcc cgcaggcaaa tcaacgacat cacctttcgg cgtaaagaca tacacccggt
     7081 cgtcaaacac ctgactacgt acttcgtcga gcatttcgcc ggaatcagcc atctcttcct
     7141 gccacgcaat cagtttacgc agccaggcaa tccggtcttc atgtcccgaa cgtgcgccgc
     7201 cagctgccgc gccctcttta tatttccagt gcgcagcaac acccaactcc gcatcttcat
     7261 gcatctgttt ggtgcggatt tggatctcaa cagtttttcc acccggcccc agaaccacag
     7321 tatgaataga ctgataaccg tttggtttcg ggttagcgac gtaatcgtca aactcatccg
     7381 gcaggtggcg atagtgagtg tgcactatcc ccagtgcggc ataacaatcc tgtaaacgct
     7441 cggcgacaat acgtaccgca cgcacatcaa acagctcatc gaaggcgagg ttctttttct
     7501 gcattttgcg ccagatgctg tagatgtgtt tcggacgacc atacacttca gctttaacgc
     7561 cttcagcttt catctcagcg cgcagatggc cgacaaactc ttcgatatag tgttcgcggt
     7621 cgagacggcg ttcatgcagc agttttgcga tgcgtttgta ttcggtcggg tggagataac
     7681 ggaagcagta atcttccagc tcccatttca gttgcccaat cccaagacgg ttcgccaacg
     7741 gcgcgtagat attggtgcac tcttttgccg ccagtacacg ttcatcttcc ggcgcatctt
     7801 ttacttcgcg cagatgagca atacgctccg ccagtttgat gactacgcag cgaaaatcat
     7861 cgaccatcgc caataacatc cggcgaacgt tatcgacctg ttcggaggaa acagaatcag
     7921 tgtgcgtcgc tttcagctgg cggatcgccg ccatatcacg cacgccgtga ataaggttaa
     7981 caaccgactt accgacgctc tcacgcagca catcttcgct gactacgttg gcatcagcca
     8041 gagggaacag cagcgccgcc cgcagcgtgt caatgtccat acttaatgtc gagaggatct
     8101 ccaccatctc aacaccacgc cacaataaca gactggcatc cggatgcccc tgcgtctgtt
     8161 gcagacaata cgcccaggtt tcggctaagc actcacacga cttctggctg gtaataccca
     8221 gacttgcgat ccatttttcc ggatcaaatt caccagcctt attgatatgt gcacttctta
     8281 ccgcaaccat cgtcctctcc tttagggacc agacctgccg aaatcggcaa atcgtaacta
     8341 tttaacgcgc gagaaaagta ccatcgattc cagatgtccc gtgtgtggga acatatccag
     8401 catcgccagt cgcgcaatgg tatatcctgc ttttaataac gcttcgctat cccgagccag
     8461 cgttgcaggg ttacaggata cataaactat acgaataggt tccagtttta taatttgctg
     8521 cataacacct gcggcacctg ctcgcgccgg gtccagcaac actttatcga agccgttttt
     8581 cgcccacggc tgctttgtga catcttcttc aagattttcg tgataaaacg tcacattctg
     8641 taagccatta agacgcgcat tctgctggcc tttttccacc agcgccggaa caccttctac
     8701 accgaccaca ctggcagctt gtgtcgccaa tggcagtgta aagttgccca taccgcagaa
     8761 cagatccagt acgcgatctt caggttgcac atccagccat tccaacgcac gcgctaccat
     8821 tttttggttc acacccgcat tgacctgaat aaaatcgcgc gggctaaaag ttaagcgcaa
     8881 cccgtttgag tcataccagg gcatctcacc agagacggtt tcgagtatct cactatcggg
     8941 ggcgagatac agatccaggc cttcagaatg cgaaaagcgt tccagttttt cgcgatctgc
     9001 cgaacttagc ggtgcggtat ggcgcaaaat catcagcgtg ccgctggttg cctgtaccag
     9061 ttcaacatga ccaagatggc gcatagcttg taagctgccc agacatgccc tgactttggg
     9121 cagcaatgct tcaagttggg gcgctaaaat ggggcattgt ttgacgtcga caatgtcact
     9181 ggagcccgct ttgcgaaacc ccatctgaag ttgctgtgtt ttcggtaagt agttcagact
     9241 taaacgcgcg cggcggcgat agccccaggg aacatcggcg atcacttcag agacatcgtg
     9301 tttcattaat cgggcgagtg ccgcactttt gcttcgctgc tgtaaatcca cgctggcgtg
     9361 ttgttgctga cagccgccgc atacgccaaa atgaggacag cgtggcgttt cgcgttccgg
     9421 gctatcgctt aaccggcgta cgactttagc gcgggcatac tgttttttat cttcagtaac
     9481 agcaacttcc gcgttttcct gcggcaataa tccggggata aataacgttt tgccgttatg
     9541 tcgcgccacg ccctgaccaa aagagtcgag gtcgttgact gaaacggtta tgatctgacg
     9601 cgtcgtcgtg cgtcgttttg cagagtagaa ttgcgccatt ggcgagactt tctcaattta
     9661 acagtgtgac cttaattgtc ccataacgga actccatgac caactacagc ctgcgcgcac
     9721 gcatgatgat tctgatcctg gcaccgaccg tccttattgg tttattgctg agtatctttt
     9781 tcgtcgtgca tcgctataac gacttgcagc gtcaactgga agatgccggt gctagcatta
     9841 ttgagccgct tgcagtttct actgaatatg gcatgagcct gcaaaatcgc gaatctatcg
     9901 gtcagttaat aagcgtactg catcgtcgcc attccgatat tgttcgcgcg atttcggttt
     9961 atgatgaaaa taaccgactc tttgtcacct ccaattttca tcttgatccc tcatcaatgc
    10021 agctcggcag caacgtgccg tttcctcgcc agctcactgt cactcgtgac ggcgatatta
    10081 tgatcctccg cacgccgatt atttctgaga gttactcccc cgacgaatcg cccagtagcg
    10141 atgccaaaaa tagtcaaaat atgttgggat atattgcgct tgagctggat cttaaatcgg
    10201 ttcgcttgca gcaatataaa gagatcttta tttcctgcgt gatgatgctg ttttgtatcg
    10261 gtattgcgct tatttttggc tggcgcttaa tgcgcgatgt aaccggtccg attcgcaaca
    10321 tggtgaatac cgtcgaccgc atccgtcgcg ggcaactcga cagccgggtg gaaggattta
    10381 tgctcggcga gctggatatg ctgaaaaacg gtatcaactc gatggcaatg tcgctggctg
    10441 cttatcacga agagatgcag cacaatatcg accaggcgac gtccgatctg cgtgaaacgc
    10501 tggagcagat ggaaattcag aacgttgagt tggatctggc gaaaaagcgc gcccaggaag
    10561 cggcgcgtat taaatccgag tttctggcaa atatgtcaca cgagctgcgt acaccactga
    10621 atggtgttat tggctttacc cgcctgacgc tgaaaacaga attaacacca acgcagcgcg
    10681 atcacctgaa tacgattgaa cgttcggcaa ataatttgct ggcaattatt aatgatgttc
    10741 tcgacttctc gaaactggaa gcaggtaagc tgattctgga aagtattcca ttcccactac
    10801 gcagcacgct ggatgaagtc gttactctgc tggcacattc ttctcacgat aaagggttgg
    10861 aactgacgct taatattaaa agcgacgtgc ctgataacgt gatcggcgac ccactgcgat
    10921 tacagcaaat catcactaac ctagtgggga atgcaattaa attcaccgag aatggcaaca
    10981 ttgatattct ggtagaaaaa cgtgcgctga gtaataccaa agtgcagatt gaagtgcaga
    11041 ttcgggatac cggcattggt attcctgaac gcgatcaatc gcgcttattc caggccttcc
    11101 gacaggctga tgccagtatt tcccgccgtc atggtggcac cgggctgggg ctggtgatta
    11161 cacaaaaact ggttaatgaa atgggcggcg atatttcgtt ccatagccag ccgaatcgcg
    11221 gttcaacttt ctggttccac attaatctcg atctgaaccc gaacattatt atcgaagggc
    11281 catccatcca gtgcctcgca ggtaagcgcc tggcctatgt cgaaccaaac tccgcagcag
    11341 cgcaatgcac gctggatatt ttaagtgaaa cgccgctgga agtggtttat agcccaacgt
    11401 tctccgcgct gcctcccgcg cattacgaca tgatgttgtt aggcatcgcg gtgaccttcc
    11461 gcgagccgct aacaatgcaa catgagcgat tagcgaaagc ggtatcgatg accgatttcc
    11521 tgatgctggc acttccttgc catgcacaag tcaatgctga aaaactcaag caagatggta
    11581 tcggcgcgtg tctgctgaaa ccattaacac ctacgcgcct gttgcctgcc ctgacggaat
    11641 tttgtcatca caaacaaaac acgcttttgc ctgtaaccga tgaaagtaag ctggcaatga
    11701 cagtcatggc ggttgatgac aaccccgcta acctgaaact tatcggcgca ttgctggaag
    11761 atatggtgca acatgtggaa ctttgcgata gcgggcatca ggcagttgaa cgggcgaaac
    11821 agatgccgtt cgatttgatc ttaatggata ttcaaatgcc ggacatggat ggcattcggg
    11881 cctgcgaact catccaccaa ctcccgcatc agcgacaaac gccggttatc gcggtaacgg
    11941 cgcatgcaat ggccgggcaa aaagagaagc tgcttggcgc agggatgagc gattatctgg
    12001 cgaaaccgat tgaagaagag cgattgcata atttgttgtt gcgctacaag cctggcagcg
    12061 gtatttcgtc tcgcgtcgtg acgcccgaag tcaacgaaat tgtggtgaac ccgaatgcga
    12121 ccctcgactg gcaactggca ctacgccagg cagcaggaaa aaccgattta gcgcgcgata
    12181 tgctgcaaat gttactcgat ttcctgcctg aagttcgcaa caaagttgag gaacagctgg
    12241 ttggagaaaa cccggaaggc ctggttgatt tgattcataa actgcatggc agttgcggct
    12301 atagcggcgt accacgtatg aagaatctct gccagctgat cgaacaacag ctacgtagcg
    12361 gtactaaaga agaagatttg gagccggagc tgctggaact gttggacgag atggataatg
    12421 tcgcgcgcga agccagcaaa attctcgggt aacggacgtc ttgtcgagta gcaatcggct
    12481 ttttatgacg ctggtttcag attggtgaca aagtgcctgt gtttatgccg gatgcggcgt
    12541 taacgcctta tccggcctac aaaaccaatt aaattcaatg aattaaaaaa tgatgtagac
    12601 ctgatacata gcacaacagg ctattttgcg tttagcatca gtctcaaacc ggctccagat
    12661 agagccggtt ttggttttct gtcttaacgc accatgcacg ggcgcttgtt atcgaacgtc
    12721 cagccaggaa tcagatactg cattcccatc gcatcgtcac gcgcgccaag cccgtgtttc
    12781 tgatacagct catgggcttt catcacttga tccatatcga tttctacacc cagccccggt
    12841 ttttctggca cctgtaccag cccgcctttg atctcaaacg gttctttggt caggcgctga
    12901 ttaccttcct gccagatcca gtgcgtatca atagcagtaa ttttacccgg tgcagcggcg
    12961 gcaacatggg taaacatcgc cagggaaata tcgaagtggt tgtttgagtg tgaaccccag
    13021 gtcaggccaa attcatggca catttgcgcc acacgtaccg aaccttgcat agtccagaaa
    13081 tgcggatccg ccagcgggat atcaacggat tgtaacgaga gcgtatggcc catctgccgc
    13141 cagtcggtgg cgatcatatt ggttgcagtc ggcaggccag tcgcgcgacg gaactctgcc
    13201 atcacttcac gcccggagaa accttgctcc gcaccacacg gatcttctgc ataagccagc
    13261 gaacctttca ggtatttacc aattttaatt gcttcgttca gcgaccaggc accgttagga
    13321 tcgagcgtaa tacgcgcctg cgggaagcgt tgcgccagtg ccacaataga ctcggcttct
    13381 tcttccccgg ccagtacacc gcctttcagt ttgaaatcgt tgaagccata tttttcatac
    13441 gccgcttccg ccaggcgcac caccgcatcc ggcgtcatcg cttcttcatg acgcaggcga
    13501 taccagtcgc atgagtcatc cggctggctt tgatacggca gaggtgtggc tttgcgatta
    13561 ccgacgaaga acagataacc gagcatttcg acttcgctac gctgttgacc atcgcccagc
    13621 agcgatgcca cgtttacccc cagatgctgc cccagcagat ccagcattgc cgcttctatc
    13681 ccggtaacta catgaatagt ggtacgcaaa tcaaatgtct gcaaaccgcg cccactagca
    13741 tcacgatcgg caaaagtatt acgcaccagc gtcagaacgt ttttgtattc acccagcgtt
    13801 ttacctacca ccagcggaat cgcatcttcc agcgttttac ggattttctc gccgccggga
    13861 atttccccta cgccagtgtg accggaatta tctttgataa tcacaatatt acgcgtgaag
    13921 aacggtgcgt gtgcaccact cagattcatc agcatactgt catgacccgc caccgggatg
    13981 acctgcattt cagtaacaac aggcgtcgta aattgagaac tcatatttat gtcctttttc
    14041 agaatcaatg acggccgaaa acgggacgtt tacggtcaaa ggtccagccg gggatcaggt
    14101 actgcatcgg acctgcgtcg ttacgcgcac cgcaaggcag acgtttatag gcctcatgtg
    14161 ccttttgtac ctgttcccag tccagttcca cgcccagacc gggcgcatca ggaacggcaa
    14221 tttttccgtt tttaatctcc agcggatttt tggtcaggcg acaatcgccc tcctgccaaa
    14281 tccagtgggt atcgatagcg gtaggattac ccggtgccgc cgcgcccaca tgggtaaata
    14341 tcgccagaga gatatcgaaa tggttgttgg aatggcagcc ccaggtcagc ccccagtcgt
    14401 cgcaaagctg cgccacgcgg actgcaccgg aaagcgtcca gaagtgcgga tcggcaagtg
    14461 gaatatctac cgcattgagc atcaccgcat gacccatttc gcgccagttg gtggcgatca
    14521 tgttagtcgc gacgggcaag ccggtcgccc gtcgaaactc cgccatcact tcacgtccgg
    14581 agaagccctg ttctgcgccg catggatctt cggcataggt aagaacatca ttcagccctt
    14641 tgcataaaga aatggcttca tcaagcagcc atgcaccgtt gggatcaacg gtaatccgcg
    14701 catccgggaa gcttttcttc aatgcacgaa cagtgtcgat ttcttgctcg ccaggtaaca
    14761 ccccgccctt aagtttgaaa tctttaaagc catagcgatc ctgtgaggct tccgccagac
    14821 gcacaacggc ttcgctgttc atcgctttct gatggcgcaa ctgataccac tcatggttgc
    14881 ccggcgtatt ttccagataa ggaagatcgg ttttggtccg atcacctata taaaacagat
    14941 aaccgaggac ggtaatagtc tcgcgttgct tgcctggccc caacagttcg cagaccggaa
    15001 cattcagcgc cttacctagc aggtcaagca aggcggcttc cagcgccgcc acggcattaa
    15061 cgcgcaattc aaaagtccag gcacctttgc cgaaggtctc aaaatcggct gcctgatttc
    15121 ctttatgtac ctgctgaacc actttattca ggcgcgcaac ttcctggccc agaaccatcg
    15181 gaatagcatc gaccagtgtt tgataaatca cctctccgcc cggcgcttca ccaatgccgg
    15241 tatgcccggc gttatcggtg agtaccacga tattgcgggt gaagtatgcg ttatgcgcac
    15301 caccaatatt gagcaacatg ctgtcatgtc cggcgaccgg aatgactttc atatcagtaa
    15361 taacaggact ggattgtgtc gccatcttat tgccccgcga caggtttcag ctcgatacgc
    15421 ttgatatcgc ccaccagcac caggtagctc agtaccgcga ttagggcatg aacaccaaca
    15481 taaatcagcg ccccattaaa cgagccagtc gtgccaacga tataaccaat tgcgattggc
    15541 gtgacgatgc cagaaatgtt accgaacatg ttgaactggc caccggaaag accgctgatc
    15601 tcttttggcg cggtatctgc cattactgcc caacccagcg ccccgatgcc cttaccgaag
    15661 aaggccagcg ccataaagcc gatgatcatc cactcaacgt tgacgtagtt gcagaacacc
    15721 atcaccatcg acagcaacat gcccattacg atcggtgttt tacgcgcaat gttcagcgat
    15781 cccgtgcggc gcatcagcca gtcggaaata attccaccca gcacaccgcc gataaaaccg
    15841 caaaccgccg gaacggaagc cacaaagccc gctttcagaa tcgacatccc acgcgcctgc
    15901 accagataaa ccggaaacca ggtaatgaaa aagtaagtca gggcgttgat acagtactga
    15961 ccgatataaa cgccgatcat catccgtgac ccgagcagct gtttgatctg cccccacttc
    16021 acgctgaacg gcactttaac tttggtgctt tgctgatcca tattgatcag cgcgccaccc
    16081 gcggcgatat actccagctc tttctgattt acccccggat gttgatttgg ctcgtggatg
    16141 actttcaacc agataaagct gatgacaatg cccagaccgc ccataaagaa gaagacgtgt
    16201 gaccagccca cttcatgcgt cagccagccc ataatcggcg cgaagatcac cgttgcgaag
    16261 tattgagcgg agttaaaaat cgacaccgcc gttccccttt cctgcgccgg aaaccaggcc
    16321 gcaacaatgc gactgttgcc ggggaaagat ggcgcttcag caagcccgac caggaagcgc
    16381 agagtaaaca gggcaacgat aatgccgaat ccactaaaga tatcgacgaa gccttgcagc
    16441 aaggtaaaca tcgaccagat aaagatcgac cagaagtaga cgcgttttga accaaaacga
    16501 tccagcaacc aaccaccagg gatctgcccg ataacataag cccatgagaa ggcagagaac
    16561 acatagccca ttcccacggg atcaaggccg atatctttgg ccatttccga accggcaata
    16621 gaaagcgtag cgcggtcgcc gtagttgaag gatgtgacga taaacaacat caccactatc
    16681 cagtaacgag cattcgtgcg tttttccaca ctgctcgcag cctgacttaa agaactcatt
    16741 gttgcactcc tgaaaattcg cgttagccac gctcactctg gactgcgaca tcgccaggaa
    16801 atcagaggtg acgtagggtg ttttttgccg tttttatagg tcgttcgccg aatacggcgc
    16861 gtgtttatat ctggcaatag cagtataaaa agcgcgccat agcggctcac cgtgcaacaa
    16921 cacaacatta atgcgttcaa tgaggcccga ttttggcatt agccctggac ggtggaatcc
    16981 acttcacgga aatgaaaaca agaacaagaa aggaagggta aaaacgaaga aataaagaag
    17041 agtatgaaat ggatcgcttg actctaggca aacgccagta aaaatccgcg ctatgaagca
    17101 gtttttactg gcatttgcct gaaaagattc gattcagcac cgctaaaacg acatttaccg
    17161 ctcgctgaca tatcaggaca acagcgtgcc ccactgttcg acccacggat ttgattccgt
    17221 ttccggttcc gggttctcac tggcgtcaat caacagcatt tcgccaaccc gctgagcgct
    17281 ctgttcctgc aacaaggcat cgaactgttt gccgccattg caaaaattta cataactgct
    17341 gtcgccgagc gcaatcacgc cataacgcag attcggctgg aagcccagac tatctttgat
    17401 tccctgaaag agcggcacaa tgctatcagg aaggtcgccc tgcccggtcg tggacgtaac
    17461 caccagaaca tacttatcct gatagggcag ccagtcgctt aattcaggat cttcaaatac
    17521 cgttgctttg tggccctgtg cggtcagaat tgcttctgct tcttccgcca ctaacagcga
    17581 atttccgtac atggtgccga caaaaatacc aatttccgcc ataccgtttt ctccctggat
    17641 taggaactta tctctccatc ctgacccgat ggcgcactga actcaaccct ttcattttca
    17701 ggaagcagac cgcgccagcc aaattgtgat aacgcctgca tccaggtgtc gtccaggccc
    17761 gcgtgaatag tcagcggctc gccagtaaaa ggatgcgtca atgacaactg actggcatgt
    17821 agcattaacc gctggaggcc aaaatgctca gcaccgctgc gattctggcg taaatctcca
    17881 tgtttgctat cgccaataat cggatgacgc aaatgggcaa gatgtcggcg aagctgatgt
    17941 ttgcgtccgg ttttcggctc cagttccacc aggccgtagc gcgtggtcgg gtaacgtccg
    18001 gtcgctaccg gcatttctac ggtcgccaga ccgcgataat gcgtcactgc tggctgcggg
    18061 cctttatctt cgcgggcaaa tttatcagcg attttgtcca gttcttccac cagtggataa
    18121 tccaacaccg cttcttccat caaccagccg cgcacaatcg catggtaacg tttctggatt
    18181 tggtgctgtt caaactgttg tgccagcagc cgtccggcct cgctggataa tcccatcaac
    18241 aacacaccag aagtgggtcg gtccagacga tgaacagtaa aaacatgctg tcctatctgg
    18301 tcacgcacgg tttgcatgac cactactttc tcgtcgcgat ccagccagct acggtgaacc
    18361 agccagccgg agggtttatt taccgcaacc agccattcat cctgatagag tatttccagc
    18421 attagctcgc atcatccgca aaaagagcat ccagtttttc caactcagcc agaataagcg
    18481 cgcgttgcgg atggtccgtc gctagcgcca tttcataata gggtgcaaca gcaaaagcgc
    18541 ccggtaacgg ctggttgtta tttaacaaat cgtgcattcg cgggatcagc ccccactgca
    18601 accactccag tggttccatg gtgtccataa agaacggttg ggtactatta aattgatgcg
    18661 gctggggttc atcgtttcgc cagtgctgat gttcacgcag taacgcttca agcgcctgca
    18721 actggagacg aacgcggtca tgagtggtca taaagcaacc tcaataagaa aaactgccgc
    18781 gaaggatagc aggaataaaa aaagggagca ctgtattcac agtgctcccg gttcgtttcg
    18841 cagcattcca gctacttttg ttgctccctg ctcatccttg acaacttttc ctctggcctt
    18901 gcggccaatc gttcatcctg aactattgct tcctgctcac accaccccga tgtgatactt
    18961 catcctgaag tgtccctggc cttcctgacc caccgaatca tcctgaccgg ttctcattct
    19021 ccatcctgga ggtgtccttt aacgcgtcct gcgtcatcct cttcgcttca tccagaagcc
    19081 tttccctgaa acaccatcct ggtgtgtcct gcagaagtgt catcatcctg atgttcactt
    19141 cgttgtctga ctccctgtcg acgaagatag aatcgtcttt ttcgcgaagt cttacaaggc
    19201 gcttacagac aatgccttaa ggaaaatttc atatgaaagt taagaattaa caatcaacat
    19261 tttgatttta aatgaaaata acgattctta tctttttaag tattcgctta tttcctggcg
    19321 atctcctaca gagtctgtaa gagatctctc acaaccagat ggggtatttg gattacagaa
    19381 gaggcttaag ttgattaagg aattccgcaa gatttgaggc taaatgcatc cgtttacgtg
    19441 ttcccagcgt ctctttgcac acttcaccgg agagattaca caccgagatg acctctaact
    19501 cttcttccag cgtcgcgata aatagcgtgg gcggcagttt tagccttttc tgagtcacca
    19561 gatgaccaat cagattttcc tgcacccgac ggaagtcgtc ttcactccag gtttgcagca
    19621 atgtcagttt gatatcgcca aactgcgcgt gcatatcccc ggcaaactga gtcgtataaa
    19681 aggtatgaat tgtgggttgt atcacaatat caaaagcgcg ttcaaccgcg tttacatttt
    19741 gttccccggt aaacggctgg ggttgccagt atacggcatc ttcggtagtt gaaataatgc
    19801 acggtgaagg aacaccgtac agttcctcac ttaacggcca gcttttatgc tcttcatgcc
    19861 acgcatcgca gtagcgtgca gtaaaatctt tcagggcttg tgcggtcaaa tcgtccacta
    19921 atttctctct tcacttaaac cagatacact tgtcgcttag tttatctggt ttatgacggt
    19981 gaaacatgtc ttcttatgca aaccatcagg cacttgcggg cctgactctt ggaaaatcaa
    20041 ccgattaccg ggatacctat gacgccagcc tactgcaagg cgttccacgc agcctgaatc
    20101 gcgacccgct gggtctgaaa gcggataacc tgccttttca cggtacggat atctggacgc
    20161 tgtatgaact ttcctggctg aatgcgaaag gtttgccgca ggtcgctgtc ggtcatgttg
    20221 aacttgatta caccagcgta aatctgattg agtcgaagag ttttaagctc tatctcaaca
    20281 gttttaacca gacgcgtttt aataactggg atgaggtgcg ccagacgctg gagcgcgact
    20341 taagcacttg cgctcagggt aagattagcg tggcgttata tcgtcttgat gaactggaag
    20401 gccagccgat aggtcatttc aatggcactt gcattgatga ccaggatatc actatcgata
    20461 actatgaatt cactactgac tatctggaga atgccaccag cggtgaaaaa gtagtggaag
    20521 agacgctcgt cagccacctg ctgaaatcaa actgcctgat cacccatcaa ccagattggg
    20581 gttcgatcca aattcagtat cgtggacgcc aaattgacag agaaaaactg ctgcgttacc
    20641 tggtctcatt ccgtcatcac aacgagttcc acgaacagtg cgtggaacgc atctttaatg
    20701 acctgttacg cttctgccag ccagagaaat tgagcgttta cgcacgttat acccgtcgtg
    20761 gcggtctgga cattaacccg tggcgcagta atagcgattt tgtcccatcg accacaagac
    20821 tggttcggca ataaattttt tctcaatttt gcgtgctgga ttcacgcaga aggttgtgaa
    20881 aggtcatcag gcagggctat tgtaatcaaa gggaatgacg atattcgtcc cataaggagt
    20941 ttttcttgat tacacatatt agcccgcttg gctccatgga tatgttgtcg cagctggaag
    21001 tggatatgct taaacgcacc gccagcagcg acctctatca actgtttcgc aactgttcac
    21061 ttgccgtact gaactccggt agtttgaccg ataacagcaa agaattgctg tctcgttttg
    21121 aaaatttcga tattaacgtc ctgcgccgtg aacgcggcgt aaagctggaa ctgattaatc
    21181 ccccggaaga ggcttttgtc gatgggcgaa ttattcgcgc tttgcaggcc aacttgttcg
    21241 cggttctgcg agacattctc ttcgtttacg ggcaaatcca taataccgtt cgttttccca
    21301 acctgaatct cgacaactcc gtccacatca ctaacctggt cttttccatc ttgcgtaacg
    21361 ctcgcgcgct gcatgtgggt gaagcgccaa atatggtggt ctgctggggc ggtcactcaa
    21421 ttaacgaaaa cgagtatttg tatgcccgtc gcgtcgggaa ccagctgggc ctgcgtgagc
    21481 tgaatatctg caccggctgt ggtccaggag cgatggaagc gccgatgaaa ggtgctgcgg
    21541 tcggacacgc gcagcagcgt tacaaagaca gtcgttttat tggtatgaca gagccgtcga
    21601 ttatcgccgc tgaaccgcct aacccgctgg tcaacgaatt gatcatcatg ccggatatcg
    21661 aaaaacgtct ggaagcgttt gtccgtatcg ctcacggcat cattatcttc cctggcggtg
    21721 tgggtacggc agaagagttg ctgtatttgc tgggaatttt aatgaacccg gccaacaaag
    21781 atcaggtttt accattgatc ctcaccggcc cgaaagagag cgccgactac ttccgcgtac
    21841 tggacgagtt tgtcgtacat acgctgggcg aaaacgcgcg ccgccattac cgcatcatca
    21901 ttgatgacgc cgctgaagtc gctcgtcaga tgaaaaaatc gatgccgctg gtgaaagaaa
    21961 atcgccgtga tacaggcgat gcctacagct ttaactggtc aatgcgcatt gcgccagatt
    22021 tgcaaatgcc gtttgagccg tctcacgaga atatggctaa tctgaagctt tacccggatc
    22081 aacctgttga agtgctggct gccgacctgc gccgtgcgtt ctccggtatt gtggcgggta
    22141 acgtaaaaga agtcggtatt cgcgccattg aagagtttgg tccttacaaa atcaacggcg
    22201 ataaagagat tatgcgtcgt atggacgacc tgctacaggg ttttgttgcc cagcatcgta
    22261 tgaagttgcc aggctcagcc tacatccctt gctacgaaat ctgcacgtaa tctcccctcc
//
LOCUS       DAJTNM010000061        21830 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTNM010000061 DAJTNM010000000
VERSION     DAJTNM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.061"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..157
                     /locus_tag="OO172_002379"
     CDS             <1..157
                     /locus_tag="OO172_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5512538.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
     gene            227..1033
                     /locus_tag="OO172_002380"
     CDS             227..1033
                     /locus_tag="OO172_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5512539.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1043..1450
                     /locus_tag="OO172_002381"
     CDS             1043..1450
                     /locus_tag="OO172_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5512540.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1450..1944
                     /locus_tag="OO172_002382"
     CDS             1450..1944
                     /locus_tag="OO172_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5512541.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2010..2807
                     /locus_tag="OO172_002383"
     CDS             2010..2807
                     /locus_tag="OO172_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5512542.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2818..3606
                     /locus_tag="OO172_002384"
     CDS             2818..3606
                     /locus_tag="OO172_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5512543.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3658..4887
                     /gene="sorE"
                     /locus_tag="OO172_002385"
     CDS             3658..4887
                     /gene="sorE"
                     /locus_tag="OO172_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5512544.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMELSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5269..5958
                     /gene="pepE"
                     /locus_tag="OO172_002386"
     CDS             5269..5958
                     /gene="pepE"
                     /locus_tag="OO172_002386"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5512545.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6049..7680)
                     /locus_tag="OO172_002387"
     CDS             complement(6049..7680)
                     /locus_tag="OO172_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5512546.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7900..11583)
                     /gene="metH"
                     /locus_tag="OO172_002388"
     CDS             complement(7900..11583)
                     /gene="metH"
                     /locus_tag="OO172_002388"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5512547.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISSDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTMPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11783..12607
                     /gene="iclR"
                     /locus_tag="OO172_002389"
     CDS             11783..12607
                     /gene="iclR"
                     /locus_tag="OO172_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5512548.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12924..15108
                     /gene="espL4"
                     /locus_tag="OO172_002390"
                     /pseudo
     CDS             12924..15108
                     /gene="espL4"
                     /locus_tag="OO172_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="OO172_002391"
     CDS             complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="OO172_002391"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR5512549.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="OO172_002392"
     CDS             complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="OO172_002392"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5512550.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGESMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="OO172_002393"
     CDS             complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="OO172_002393"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR5512551.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20201..21130)
                     /gene="metA"
                     /locus_tag="OO172_002394"
     CDS             complement(20201..21130)
                     /gene="metA"
                     /locus_tag="OO172_002394"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5512552.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21287..21727
                     /locus_tag="OO172_002395"
                     /pseudo
     CDS             21287..21727
                     /locus_tag="OO172_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         5380 a         5590 c         5526 g         5334 t
ORIGIN      
        1 gctctctatc gaaatgaata aattaaagca ggcgcgatat tccattggca ttgccatgag
       61 tgaagaaaaa tacagcggaa ttattggtgc actgcgtgga aaatatatta attgtctggt
      121 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
      181 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
      241 aaatttgcag gataaaatca ttattgtcac cggcggcgca tccggtattg gtctggcgat
      301 tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc acggtggcga
      361 tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt ctagcacaaa
      421 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
      481 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
      541 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt
      601 tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa
      661 tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg ccgctaccaa
      721 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
      781 tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
      841 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
      901 aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt ttgtttgtta
      961 tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     1021 aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     1081 ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg aagccgttga
     1141 atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggaaaagt tagcaagcat
     1201 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtggaatgc
     1261 ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc tttctctgcc
     1321 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     1381 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cgacagagga
     1441 ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     1501 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     1561 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     1621 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata tcaggacgaa
     1681 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     1741 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     1801 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggt
     1861 gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     1921 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcag
     1981 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa atcattgcta
     2041 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     2101 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     2161 tactcggcgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
     2221 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     2281 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
     2341 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcagaagagg
     2401 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     2461 tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     2521 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
     2581 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     2641 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     2701 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     2761 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     2821 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     2881 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
     2941 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     3001 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga aattgatgac
     3061 ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca
     3121 ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg
     3181 ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg
     3241 aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc
     3301 gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc
     3361 gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc
     3421 gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt
     3481 ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg
     3541 gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct gggcttcctg
     3601 tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag gtggtttatg
     3661 aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt
     3721 cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct gtgcctctct
     3781 tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc
     3841 aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg taaaaagtgg
     3901 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac
     3961 cgcccggatt gccccggcta ctccttcccg tgggtaggcg gcgaagcgac gcatgtggtt
     4021 attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt
     4081 gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
     4141 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
     4201 ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca tggaccagtt
     4261 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
     4321 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
     4381 gatacgttaa tggagctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
     4441 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
     4501 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
     4561 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
     4621 aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
     4681 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
     4741 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
     4801 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata cctgctcgcc
     4861 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
     4921 tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca caatttgcac
     4981 gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtacacat
     5041 tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga aaatccaata
     5101 tattgcagtt atctggtagg ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca
     5161 tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat
     5221 tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
     5281 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
     5341 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
     5401 acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
     5461 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt
     5521 gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct
     5581 ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc aggcgctaac
     5641 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
     5701 gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc gctgccggaa
     5761 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
     5821 ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
     5881 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tccgctggaa
     5941 gctggtcacc gtttttaagt gaattgccgg atgcatgatc cggcatcttg ttcatagtgc
     6001 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
     6061 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     6121 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
     6181 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     6241 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
     6301 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     6361 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt cgctgcccat
     6421 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     6481 gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat acagcttaat
     6541 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
     6601 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     6661 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc
     6721 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     6781 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
     6841 gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca ttgtctctgc
     6901 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
     6961 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     7021 ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
     7081 agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
     7141 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
     7201 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
     7261 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     7321 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
     7381 gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga ccagagccgg
     7441 tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
     7501 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     7561 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
     7621 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
     7681 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
     7741 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
     7801 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
     7861 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
     7921 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
     7981 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa
     8041 ataccaaccc gaaaccgatg caccaggcca catagcgaaa gattctgtga gtttcatgcc
     8101 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
     8161 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
     8221 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
     8281 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
     8341 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
     8401 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
     8461 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
     8521 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
     8581 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
     8641 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
     8701 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
     8761 cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt
     8821 gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
     8881 tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg
     8941 agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac caacggtgcg
     9001 cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt
     9061 gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggtgaagc cctgacgctc
     9121 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc
     9181 aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag gcaccataac
     9241 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
     9301 gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg ttttaccctg
     9361 ctctttgctg gcttcaataa acggttcgag atacgccact gcctgtttca tgacgcgcgc
     9421 cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc cgaccacgtt
     9481 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
     9541 ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt attccagacg
     9601 tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt
     9661 actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt cacgacgatt
     9721 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
     9781 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
     9841 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa cgttagatac
     9901 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
     9961 gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga tgttcgggtc
    10021 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    10081 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac
    10141 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc
    10201 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    10261 aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    10321 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    10381 gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat ccagcgcctc
    10441 gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga cgttggtgcg
    10501 ttcacccacg ttcacaaaca ggctatcatc gccaatgttc agcggctcca ggccagacaa
    10561 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    10621 catcgctgca atatgttgcg gcatggtccc gcagcaacca ccgacgatat tgagaaagcc
    10681 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc
    10741 aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg caatccgtga
    10801 tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    10861 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    10921 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    10981 ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt
    11041 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt cgcgataggc
    11101 cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt ccgaagaaat
    11161 agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt tctccggcgt
    11221 gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc gccgccgcaa agttgatttc
    11281 cgccgacagg gattccatct ggtaatccac catcgcaatg gtcgtggagt tgaaggtgtt
    11341 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    11401 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    11461 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    11521 gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    11581 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    11641 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    11701 catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa taatttccat
    11761 gatacagaaa aaggaggctg tcatggtcgc ccccattccc gcgaaacgcg gcagaaaacc
    11821 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    11881 actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgg cgcaacaagc
    11941 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    12001 gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    12061 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
    12121 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    12181 tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta tcggcggtaa
    12241 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
    12301 ggtgactaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa ctctggtgtc
    12361 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
    12421 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgcga
    12481 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg atcgcgtgac
    12541 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    12601 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    12661 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    12721 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    12781 caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg atttatgata
    12841 tttttttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa attcaagaca
    12901 tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct ttctctgcaa
    12961 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
    13021 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
    13081 agaactgtag acaaaatgtt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
    13141 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
    13201 cgacactcac tcaaaccatt gctacaatat ttgaagaata cctaattgaa attttacctc
    13261 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
    13321 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgcccc aaaaccgtat
    13381 agtataccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgattacaa
    13441 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
    13501 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg ttgctgcctg
    13561 cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa atggcatcag
    13621 tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc taagaataaa
    13681 gaacacaact gaaggatgta cccattacgt gatttcggtt tatgatccta atgtaactaa
    13741 cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga
    13801 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc
    13861 tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag gaactgtgga
    13921 tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa
    13981 tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga
    14041 aatgttgact gcctataata gtattaacct ccccggttta tatttggcta taaattatgg
    14101 caatgcggat atcgttgaga ctattttcaa ttcattgtca gaaacaggat atgaagggtt
    14161 actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg gtttttctgg
    14221 attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc tgaacgtctt
    14281 acctaaactg gccgcaacac atcatttaga taatgaacag gtgtataaat tcctgagtgc
    14341 caaaaataga acgtccagcc atgttttata ccatgttatg gcgaatggtg atgccgacat
    14401 gctgaaaatt gttttggtcg cgttaccttt gttaattcgc acatgtcatt tgactaaaga
    14461 acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaagac tatacctggc
    14521 gatgcaaaat ggacatagct atatcgtgaa agttattctc gaagcattgc cctgtctggc
    14581 ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta aaagtcttgc
    14641 gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta ttaatactat
    14701 ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa tatgaagccc
    14761 ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat acagcataaa
    14821 caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg cctgtttgga
    14881 tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa atactctgcc
    14941 tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct taatacatta
    15001 aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc ggagaaaaat
    15061 tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat ctttgcctgc
    15121 gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt agtgcgcgcc
    15181 agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca atacgcgggt
    15241 cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag acgctgtacc
    15301 acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg cggaaattca
    15361 cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg tgacgggtga
    15421 caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt agcagcggca agctggcgaa
    15481 tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact tgttccagcc
    15541 agatattgag cggcaccatc cgccgctcga tatagagatg gcgaattaca atctgctcac
    15601 caagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat accggggaaa
    15661 tatgccgctt ctccagcaca aagttttcaa actcctgggt gtccgccatt cggcccacac
    15721 ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac atctctttct
    15781 gcggcgcgaa cttgtctttg atgactttga atacccgatc aaagcccggc agcgtaaaca
    15841 ccaacatcac cattccacga atacccggcg cttcaatgaa ctgctcatta cagccctgta
    15901 gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag ccgatagcca
    15961 tatacaattc agcagtggtt ttacctggca gaatttcccg tagccactcg accagcgctg
    16021 cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt
    16081 cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc tggtggatcg
    16141 gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc caggcggctt
    16201 tattgcggta aaacagttcg ttcgccacct gtaaatgact ttccgcgagg ttgtctgtcc
    16261 ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc acggctttta ttctgccagc
    16321 gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag agattcccag ccgtgatcgg
    16381 ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct ggctgagagc
    16441 taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga cagtacacgg
    16501 agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc cgcgtgtaat
    16561 gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta atgcagcgca
    16621 gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt ttcatcgcct
    16681 gctggacagc atgccagtcg gcctgttcga aacgctgctg cgccccggaa gtcacttcga
    16741 ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca
    16801 ggccacgcgg catttagcgc actcatcagg agcagagaat tgccggatgc ggcgtaaaca
    16861 ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca agcgtcgcat
    16921 caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc tacagtcagc
    16981 aacgtttgta ggttgcttag aactgcgatt cttcagtgga gccagtcagc gcggtgactg
    17041 aagacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta cccacttcct
    17101 gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc tgctgcactt
    17161 tctcaacata gtgcttcata ctttcgccct gcgcataagc gtttgccagg tcgaacatgt
    17221 taaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag cccatatccg
    17281 acaactgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg
    17341 acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata gcttgtgcaa
    17401 agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg tcagcatatg
    17461 gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc
    17521 cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa tctgaggtga
    17581 tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg cccgtcacgt
    17641 cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc actaaaactt
    17701 tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg
    17761 cagcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac
    17821 cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct
    17881 caatgcccac ggaccattgg atctgatcgg cacgacggaa ggtgttgttg atccgctcca
    17941 ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca
    18001 ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg
    18061 cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg
    18121 actcaccgtg cagcagacgc cacattttcg ctgcgcccag ttgcgccagc gtgcattcag
    18181 gattgactga accgcgtaat ttcaccacat cttccgcact gtatgggcga gtaatgcctt
    18241 cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca
    18301 tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt
    18361 caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc
    18421 aaaacggccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc
    18481 gcccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca acgttttttg
    18541 atgatggatc cactgccaga tcgaggtacg ggaaatttca gccgtcgccg catcttccat
    18601 caggccataa atcggcacgc agccgttgcc ggagatccac gcttcgatgt actgcacagc
    18661 cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag gtgccagcag
    18721 ctgatcggca gtaatcggcg cgtcttgttc gcgcaatact tcaagctgat ttttacggga
    18781 gccgagaata tcgttgaata ccgccattgc cgtgtccgca aggcctgggt gagcgatcca
    18841 tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt tgttgagcac
    18901 ctggttatta cgctcttcat ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa
    18961 agcaccgcgt ttatggcagg ttttaatcag caggcgtgag taagcattca ggaatggttt
    19021 atccatcgtc acggcctgtc tgtctggcag gacacgatcg ggatagtttt tcaacgtttt
    19081 gatatagctg aagatgtaat cccagcgacc gcagttcaga ccaacaatat ggtcgcgcag
    19141 cgcgtgaagg atttcgtcca tctggaacac cgcgggcagc gtttcaatca gcaacgtcgc
    19201 cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca
    19261 ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac cgcagccctt
    19321 tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctaccggg
    19381 gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca
    19441 aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat tggtatagct
    19501 gatggtaccg ttaaccgcat cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc
    19561 tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt tcagtgcgtt
    19621 gatcaccatc ttgcgctcta ccgggccagt tatctctacg cggcggtctt ctaagtccgc
    19681 aggaatcccg cgaattttcc aatcagcatc gcgaatggaa gccgtttccg aaataaaatc
    19741 aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt
    19801 gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag
    19861 aatttgcttc tcctgctcgc catacggcct tgtgaaagcc agttcatcgg ttgttgttgc
    19921 ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttcccctc tgtgaacgaa
    19981 ggaatgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa tgcaaaaaca
    20041 aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa tatgaataaa
    20101 atttattcac tttctgaggt gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg
    20161 taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca
    20221 tgtgccgtag atcgtatggc gtgatctgat agacgtaata gttgagccag ttggtaaaca
    20281 gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatcg ttgtgcggga
    20341 aatagttata cggtacatcc gggtctagtc cggcttccac atcgcggaaa tattcctgcg
    20401 ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc
    20461 tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtgtaat
    20521 cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg aatgaatcat
    20581 caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga
    20641 gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg
    20701 cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga
    20761 tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca ccggttacaa
    20821 tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag ttgttcagat
    20881 gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga atatcgacct
    20941 gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca
    21001 tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg
    21061 tcatcacaaa cacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac
    21121 gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc
    21181 tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca
    21241 aggaaaaggc tacgttagaa tataagaatg acgaaaagga gagaaaatgg ttattagtat
    21301 tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc gatctgtcga
    21361 tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc tggtgcgttc
    21421 ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggagcagc cggttgggtc
    21481 tatgttgtta agtgggcagc atatggatgc gctgtttatc gatcctgatg tgtgcggctg
    21541 cggcgtgggt cggatgctgg tggagcatgc gctctcgatg gcaccggaac tgacaaccaa
    21601 cgttaatgag caaaatgagc aggcggttgg gttctataag aaggtgggtt ttaaggttac
    21661 gggacgctct gaggtggacg atttggggaa accgtatccg ttgctgaatc tggcgtatgt
    21721 gggggataag ccgcccctgc cgtgcctgcc caagctggag aagacgcgcc agcgtcgcac
    21781 ccggcatttt tttcaccaac gcaaaaaggc catcctgacg gatagccttt
//
LOCUS       DAJTNM010000062        21516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTNM010000062 DAJTNM010000000
VERSION     DAJTNM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.062"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO172_002396"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO172_002396"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5512553.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYAVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO172_002397"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO172_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5512554.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLHD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO172_002398"
     CDS             complement(2649..3200)
                     /locus_tag="OO172_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5512555.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO172_002399"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO172_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5512556.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="OO172_002400"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="OO172_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR5512557.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMEILNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OO172_002401"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OO172_002401"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5512558.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OO172_002402"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OO172_002402"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5512559.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OO172_002403"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OO172_002403"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5512560.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OO172_002404"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OO172_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OO172_002405"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OO172_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5512561.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="OO172_002406"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="OO172_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5512562.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="OO172_002407"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="OO172_002407"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5512563.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OO172_002408"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OO172_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5512564.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OO172_002409"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OO172_002409"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5512565.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OO172_002410"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OO172_002410"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5512566.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSGNTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OO172_002411"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OO172_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5512567.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="OO172_002412"
     CDS             complement(18174..18569)
                     /locus_tag="OO172_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5512568.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OO172_002413"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OO172_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5512569.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="OO172_002414"
     CDS             19485..20078
                     /locus_tag="OO172_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5512570.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="OO172_002415"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="OO172_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5512571.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20798..>21516
                     /locus_tag="OO172_002416"
     CDS             20798..>21516
                     /locus_tag="OO172_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5512572.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5201 a         5329 c         5663 g         5323 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccaccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc gcgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcacgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaat
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccaacgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg ggaggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tgccgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtt actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg agcaaaatat cagatttgaa
    20821 ttattctcaa cacattacat tagccgacaa ttttaaacaa aaaaatgaag ccttagatac
    20881 ctggtatgtg ggaatgaatg attttgcccg aattgccggg gggcagaata gcagaagcaa
    20941 tattctttct cctagagcat ttttggagtt tttggcaaag atatttaccc taggttatgt
    21001 ggattttagc aaacgctcca acgaagcggg tagaaatatg atggctcata ttgagttctc
    21061 atcttattct aaagatactg atggcaatga aaaaatgaag ttttacatga ataatcctga
    21121 aggggagcga gcggatttat ctaaggtgaa gatagaaatt acacttgcct cagcttctac
    21181 taaagggatt cgtgaaggac atacagtcat tatatttaaa caatctgatg gttcgactaa
    21241 tcgttatgaa gggaagtctt ttgaaagaaa agatgatagt tcattacacc tgattactaa
    21301 caaggttctg gcgtgttacc aacgcgaagc taacaagaaa atagcgcggc tattaaataa
    21361 tcatcagaag ttaaataata tacaggagtt aaatgattcg caggagttaa ataattcaca
    21421 gaagttaaat aattcgcagg agttaaataa ctcgcaggtg agttgtaaag gttcagttga
    21481 ttctacgatt acggatttat tagaaaaagc attgaa
//
LOCUS       DAJTNM010000063        21315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTNM010000063 DAJTNM010000000
VERSION     DAJTNM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.063"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO172_002417"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO172_002417"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5512573.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO172_002418"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO172_002418"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5512574.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OO172_002419"
     CDS             complement(3121..3420)
                     /locus_tag="OO172_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5512575.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OO172_002420"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OO172_002420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5512576.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OO172_002421"
     CDS             complement(4632..5069)
                     /locus_tag="OO172_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5512577.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OO172_002422"
     CDS             complement(5115..5456)
                     /locus_tag="OO172_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512578.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="OO172_002423"
     CDS             complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="OO172_002423"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5512579.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="OO172_002424"
                     /pseudo
     CDS             complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="OO172_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8838..9830
                     /gene="xylF"
                     /locus_tag="OO172_002425"
     CDS             8838..9830
                     /gene="xylF"
                     /locus_tag="OO172_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5512580.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEREL"
     gene            9908..11449
                     /gene="xylG"
                     /locus_tag="OO172_002426"
     CDS             9908..11449
                     /gene="xylG"
                     /locus_tag="OO172_002426"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5512581.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11427..12608
                     /gene="xylH"
                     /locus_tag="OO172_002427"
     CDS             11427..12608
                     /gene="xylH"
                     /locus_tag="OO172_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5512582.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12686..13216
                     /locus_tag="OO172_002428"
     CDS             12686..13216
                     /locus_tag="OO172_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5512583.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13328..13864
                     /locus_tag="OO172_002429"
     CDS             13328..13864
                     /locus_tag="OO172_002429"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5512584.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGTRQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13972..14796)
                     /locus_tag="OO172_002430"
     CDS             complement(13972..14796)
                     /locus_tag="OO172_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5512585.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="OO172_002431"
                     /pseudo
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="OO172_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="OO172_002432"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="OO172_002432"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5512586.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGETELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVAFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="OO172_002433"
     CDS             complement(18728..19201)
                     /locus_tag="OO172_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5512587.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="OO172_002434"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="OO172_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5512588.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20352..>21315
                     /gene="yiaK"
                     /locus_tag="OO172_002435"
     CDS             20352..>21315
                     /gene="yiaK"
                     /locus_tag="OO172_002435"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5512589.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITV"
BASE COUNT         5357 a         5440 c         5230 g         5288 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     5881 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     5941 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     6001 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     6061 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     6121 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcggccc
     6181 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     6241 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     6301 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     6361 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     6421 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcgg
     6481 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     6541 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     6601 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     6661 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     6721 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     6781 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     6841 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     6901 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     6961 aaagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     7021 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     7081 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     7141 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg
     7201 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     7261 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     7321 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     7381 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     7441 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     7501 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     7561 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     7621 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     7681 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     7741 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctagaa
     7801 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     7861 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     7921 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     7981 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     8041 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     8101 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     8161 atccacatcg tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac
     8221 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     8281 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc
     8341 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     8401 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     8461 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     8521 atattgggca ctccctttca attgctcaat tatgttattt cacactgcta ttgagataat
     8581 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     8641 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     8701 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     8761 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     8821 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     8881 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     8941 tccgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg
     9001 gtgcgaaagt atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg
     9061 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccgtataac ggtcaggtat
     9121 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     9181 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     9241 tgcaggcaaa agccttggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     9301 cgccggtaga taacaacgcc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     9361 acgttgattc tggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     9421 aaaacgcatt gaaaattatg gaaaacgcgc taaccgccaa taataacaaa attgatgctg
     9481 tagttgcctc aaacgatgcc accgcaggtg gggcaattca ggcattaagc gcgcaaggtt
     9541 tatcagggaa agtagcaatt tccggccagg atgcggatct cgcaggtatt aaacgtattg
     9601 ctgccggtac gcaaactatg acggtgtata aacctattac attgctggca aatactgccg
     9661 cagaaattgc cgttgaattg ggcaatggtc aggagccaaa agcagatacc tcactgaata
     9721 atggcctgaa agatgtcccc tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca
     9781 tcaaagatac ggtaattaaa gacggattcc acaaagagag agagctgtaa gcgttacgcc
     9841 ccggcgcgga gcgggggcgt gatttctctc catgccgcgt gaatgaattg gcttaggtgg
     9901 agtcgttatg ccttatctac ttgaaatgaa gaacattacc aaaaccttcg gcagtgtgaa
     9961 ggcgattgat aacgtcagct tgcggttgaa tgctggcgaa atcgtctcac tctgtgggga
    10021 aaatgggtct ggtaaatcaa cgctgatgaa agtgctgtgt ggtatttatc cccatggctc
    10081 ctacgaaggc gaaattattt ttgcgggaga agagattcag gcgagtcaca tccgcgatac
    10141 cgaacgcaaa ggtatcgcca tcattcacca ggaattggcc ctggtgaaag aattgaccgt
    10201 gctggaaaat atcttcctgg gtaacgaaat aacccacaat ggcattatgg attatgacct
    10261 gatgacgcta cgctatcaga agctgctcgc acaggtcagt ttatccattt cacctgatac
    10321 ccgcgttggc gatttagggc ttgggcaaca acaactggtt gaaattgcca aggcacttaa
    10381 taaacaggtg cgcttgttaa ttctcgatga accgacagcc tcattaactg agcaggaaac
    10441 gtcggtttta ctggatatta ttcgcgatct acaacagcac ggtatcgcct gtatttatat
    10501 ttcgcacaaa ctcaacgaag tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg
    10561 acagcacatt ggtacgcgtg atgctgccgg aatgagtgaa gacgatatta tcaccatgat
    10621 ggtcgggcga gagttaaccg cgctttaccc taatgaacca cataccaccg gagatgaaat
    10681 attacgtatt gaacatctga cggcatggca tccggtcaat cgtcatatta aacgagttaa
    10741 tgatgtctcg ttttccctga aacgtggcga aatactgggt attgccggac tcgttggtgc
    10801 cggacgtacc gagaccattc agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa
    10861 aatttatatt gatggcaaac aggtagatat tcgtaactgt cagcaagcca tcgcccaggg
    10921 gattgcgatg gtacccgaag acagaaagcg cgacggcatc gttccggtaa tggcggttgg
    10981 taaaaatatt accctcgccg cactcaataa atttaccggt ggcattagcc agcttgatga
    11041 cgcggcagag caaaaatgta ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc
    11101 ccccgacctt gctattggac gtttgagcgg cggcaatcag caaaaagcga tcctcgcccg
    11161 ctgtctgtta cttaacccgc gcatcctcat tcttgatgaa cctaccaggg gtattgatat
    11221 tggcgcgaaa tatgagatct acaaattaat taaccaactc gtccagcagg gtattgccgt
    11281 tattgtcatc tcttccgaat tacctgaagt gctcggcctt agcgatcgtg tactggtgat
    11341 gcatgaaggg aaactaaaag ccaacctgat aaatcataac ctgactcagg agcaggtgat
    11401 ggaagccgca ttgaggagcg aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg
    11461 ccgtaccgac atccggtggc ttttctgggc tgaaatcact gaatttgcag gtcttcgtga
    11521 tgattgcagc tatcatcgca atcatgctgt tctttacctg gaccaccgat ggtgcctact
    11581 taagcgcccg taacgtctcc aacctgttac gccagaccgc gattaccggc atcctcgcgg
    11641 taggaatggt gttcgtcata atttctgctg aaatcgacct ttccgtcggc tcaatgatgg
    11701 ggctattagg tggcgtcgcg gcgatttgtg acgtctggtt aggctggcct ttgccactta
    11761 ccatcattgt gacgttggtt ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg
    11821 cgtaccgcaa agtcccttca tttattgtca ccctcgcggg catgttggca tttcgcggca
    11881 tactcattgg catcaccaac ggcacgactg tatcccccac cagcgccgcg atgtcacaaa
    11941 ttgggcaaag ctatctcccc gccagtaccg gcttcatcat tggcgcgctt ggcttaatgg
    12001 cttttgttgg ttggcaatgg cgcggaagaa tgcgccgtca ggctttgggt ttacagtctc
    12061 cggcctctac cgcagtagtc ggtcgccaag ctttaaccgc tatcatcgta ttaggcgcaa
    12121 tctggctgtt gaatgattac cgtggcgttc ccactcctgt tctgctgctg acgttgctgt
    12181 tactcggcgg aatgtttatg gcaacgcgga cggcatttgg acgacgcatt tatgccatcg
    12241 gcggcaatct ggaagcagca cgtctctccg ggattaacgt tgaacgcacc aaacttgccg
    12301 tgttcgcgat taacggatta atggtagcca tcgccggatt aatccttagt tctagacttg
    12361 gcgctggttc accttctgcg ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga
    12421 ttggcggcac cagcctggct ggcggtgtgg gaagcgttgc cggagcagta atgggggcat
    12481 ttatcatggc ttcactggat aacggcatga gtatgatgga tgtaccgacc ttctggcagt
    12541 atatcgttaa aggtgcgatt ctgttgctgg cagtatggat ggactccgca accaaacgcc
    12601 gttcttgatt ttgataaaaa ttttctcaaa gccggttacg tattaccggt tttgagtttt
    12661 tgcatgattc agcaggaaaa gaaccatgtt tactaaacgt caccgcatca cattactgtt
    12721 caatgccaat aaagcctatg accggcaggt agtagaaggc gtaggggaat atttacaggc
    12781 atcacaatca gaatgggata ttttcattga agaagatttc cgcgcccgca ttgataaaat
    12841 caaggactgg ttaggagatg tcgtcattgc cgacttcgac gacaaacaga tcgagcaagc
    12901 gctggctgat gtcgacgtcc ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag
    12961 ttacccaccc gttcattaca ttgccaccga taactatgcg ctggttgaaa gcgcattttt
    13021 gcatttaaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatcta catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggca cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggcc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctag ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggga aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gcttttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag cgcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagctt gggcagaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga gcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgtg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagtttcgc atattgaaat tttaagacaa aaaagcgatc aaaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttg
//
LOCUS       DAJTNM010000064        21208 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTNM010000064 DAJTNM010000000
VERSION     DAJTNM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.064"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..66
                     /locus_tag="OO172_002436"
     CDS             <1..66
                     /locus_tag="OO172_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5512590.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
     gene            82..933
                     /locus_tag="OO172_002437"
                     /pseudo
     CDS             82..933
                     /locus_tag="OO172_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin"
     gene            1172..1231
                     /locus_tag="OO172_002438"
     CDS             1172..1231
                     /locus_tag="OO172_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5512591.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1548..1607
                     /gene="ibsE"
                     /locus_tag="OO172_002439"
     CDS             1548..1607
                     /gene="ibsE"
                     /locus_tag="OO172_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR5512592.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1727..3160)
                     /gene="hldE"
                     /locus_tag="OO172_002440"
     CDS             complement(1727..3160)
                     /gene="hldE"
                     /locus_tag="OO172_002440"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5512593.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3208..6048)
                     /gene="glnE"
                     /locus_tag="OO172_002441"
     CDS             complement(3208..6048)
                     /gene="glnE"
                     /locus_tag="OO172_002441"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5512594.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6071..7372)
                     /gene="ygiF"
                     /locus_tag="OO172_002442"
     CDS             complement(6071..7372)
                     /gene="ygiF"
                     /locus_tag="OO172_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5512595.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7614..8234
                     /locus_tag="OO172_002443"
     CDS             7614..8234
                     /locus_tag="OO172_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5512596.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8298..9536
                     /gene="cca"
                     /locus_tag="OO172_002444"
     CDS             8298..9536
                     /gene="cca"
                     /locus_tag="OO172_002444"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5512597.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9699..10520)
                     /gene="bacA"
                     /locus_tag="OO172_002445"
     CDS             complement(9699..10520)
                     /gene="bacA"
                     /locus_tag="OO172_002445"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5512598.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10611..10979)
                     /gene="folB"
                     /locus_tag="OO172_002446"
     CDS             complement(10611..10979)
                     /gene="folB"
                     /locus_tag="OO172_002446"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5512599.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11084..11701
                     /gene="plsY"
                     /locus_tag="OO172_002447"
     CDS             11084..11701
                     /gene="plsY"
                     /locus_tag="OO172_002447"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5512600.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11714..12645)
                     /gene="ttdR"
                     /locus_tag="OO172_002448"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11714..12645)
                     /gene="ttdR"
                     /locus_tag="OO172_002448"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12852..13695
                     /gene="ttdA"
                     /locus_tag="OO172_002449"
                     /pseudo
     CDS             12852..13695
                     /gene="ttdA"
                     /locus_tag="OO172_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13692..14297
                     /gene="ttdB"
                     /locus_tag="OO172_002450"
     CDS             13692..14297
                     /gene="ttdB"
                     /locus_tag="OO172_002450"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5512601.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14345..15742
                     /gene="ttdT"
                     /locus_tag="OO172_002451"
     CDS             14345..15742
                     /gene="ttdT"
                     /locus_tag="OO172_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5512602.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALMLLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNN
                     TGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRYFFASATAYTSALAPMMIAAALA
                     MPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLV
                     LLVITGLLWMPVVLL"
     gene            complement(15785..16798)
                     /gene="tsaD"
                     /locus_tag="OO172_002452"
     CDS             complement(15785..16798)
                     /gene="tsaD"
                     /locus_tag="OO172_002452"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5512603.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17036..17251
                     /gene="rpsU"
                     /locus_tag="OO172_002453"
     CDS             17036..17251
                     /gene="rpsU"
                     /locus_tag="OO172_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5512604.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17362..19107
                     /gene="dnaG"
                     /locus_tag="OO172_002454"
     CDS             17362..19107
                     /gene="dnaG"
                     /locus_tag="OO172_002454"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5512605.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19302..21143
                     /gene="rpoD"
                     /locus_tag="OO172_002455"
     CDS             19302..21143
                     /gene="rpoD"
                     /locus_tag="OO172_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5512606.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5117 a         5862 c         5441 g         4788 t
ORIGIN      
        1 tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat aaaacactat
       61 cagtaagttg gagtcattac cgaacaacaa gaagcgttta tgacgcttga gcaggagcag
      121 caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga agctgaacgt
      181 cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga aacggagatc
      241 gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt tcgtattaaa
      301 gagatcgaac agcagcaggt caccgaaatc gccaaccaga cgaaatcgat cgctattgcc
      361 gccaaatcgg aacaacagtc ccaggcagaa gccgatcgtg ctaaacaagt tgccctaatc
      421 gctgccgcgc aggatgcaga aaccaaagcg gttgaactga ccgtgcgggc gaaagcagaa
      481 aaagaagccg cagagatgca ggcggcggct atcgttgagt tagccgaagc tacacgtaaa
      541 aagggtctgg cggaagcaga agcacaacgt gcgctgaacg atgctatcaa cgtactttct
      601 gatgaacaaa ccagccttaa attcaaactg gccttgttgc aggcgctacc tgcggtaata
      661 gaaaaatccg ttgagccgat gaaatctatt gacggtatca agattattca ggtcgatggt
      721 ctgaatcgtg gcagcgctgc gggtgatgca aacacgggta atgtgggggg cggaaaccta
      781 gcggaacaag cattatcagc cgctctctct taccgcacac aggctccgct gattgactcc
      841 ttgctcaatg aaattggcgt ttcaggcggt tcactggcgg cattgacttc acccttaacc
      901 tcaacaactc ccgtcgccga aaacgtagaa taaattttca gctatgaacg tccctggcac
      961 aaaaagccgg ggatttttta tatctgcgtt ccgctaaaaa ctgcaaatga tcaggtcgtt
     1021 gcaacgtttt gcgtgaccgc tcggggaagg caaaactgcc tctgggaaag cgttgcgcga
     1081 ggtccggcgc tcatcaacaa tcggggagtc agcaaagggc tgaaacggga aagcccctcc
     1141 cgaggaaggg gccttacaga aggaaagggt tatgatgaag ctcgtcatca tactgattgt
     1201 gttgttactc gtaagtttcg cagcttatta acagccaatc agagggggga gaaatcctcc
     1261 ctcacccttg ttcctttact ttaggttgaa aaaaaatcag cgtcaagaag gtttcattaa
     1321 cacatatccc tggcccaaaa agccggggat tttttatatc tgcgttccgc taaaaggtgc
     1381 aaatgctcag gacgttgcag cgttttgcgt gaccgctcgg ggaaggcaaa actgcttcag
     1441 ggaaagcgtt gcgcggggtc cggcgctcat caacaatcgg ggagtcagca aggggctgaa
     1501 acgggaaagc ccctcccgag gaaggggcct tatagaagga aagggttatg atgaagttcg
     1561 tcatcatact ggttgtgttg ttactgttaa gtttcccgac ttactaacaa ctgatcagag
     1621 gggggagaaa tcctccctta cccttgttcc tttactttag gttgaaaaaa caacagcgtc
     1681 aataggcctg cctgctacga agcgagatct gtgaaccgct ttccggttag ccttttttat
     1741 cctgttggat cttcttgatg atgttagtgg tcgagcaacc gtcttcgaag ttgagcacca
     1801 gcacttcgcc gccgttggcc cagacttctt tactcccggc gatctcttct ggtttatagt
     1861 cgccgccctt caccagtaaa tctggcagga tcccggcgat caagcgctgc ggcgtgtctt
     1921 cttcaaacga caccacccag tcgaccgctt ccagtgcgcc cagcacaatc atacgctgtt
     1981 cgagcgggtt aacggggcgg gaatcccctt tcagccgttt ggtggaggca tcgctgttca
     2041 cagcgacaat caagcggtca cccagtttgc gggcatttgc cagatagaag acgtgtccgg
     2101 cgtgcaggat gtcaaagacg ccattggtca tcaccacttt ttcaccgcgt ttacgcgctg
     2161 ccgctacggc cagcttcagt tcctcttcgg tcatcacgcc aaagccggta tctgcgcgcc
     2221 cacgcactgc attttccagc tcgatcggcg aaaccgtgga ggttcccagt ttgccgacca
     2281 ccacgccagc cgccgcattg gcaaagaagc aggcttcttc cagcgaatta cccgctgcca
     2341 gcgttgccgc caggacgcca atcaccgtgt cgcccgcacc ggtaacgtca tacacttcct
     2401 gcgcctgggt tggcatatgc agcggcgctt tacccggttg cagcagcgac ataccctgtt
     2461 cggaacgggt cactaacaga gccgagagtt cgtaatcggc aatcagtttc atgccgcgct
     2521 caacaatctc ttcttcggtc ttacatttac cgacaacagc ttcaaattcc gagagattcg
     2581 gtgtcagcag cgtagcgccg cggtagcgct caaaatcggt accttttgga tcaatcagca
     2641 ccggaacgcc cgctttacgc gccagttgga tcatctgctg tacgcttgcc agcgcacctt
     2701 tggcgtagtc agaaagcacc agcgcgccaa tcgaactcag cgcctgatta atccgctcgt
     2761 gcagcggctg cggatcaaca ccttcgaaac cttcttcaaa atccagacgg atcagctgtt
     2821 ggttgcggga aagtacccgt aatttggtga tggtcggatg cgtcggtaca gaaacgaagt
     2881 cgcatttgac gttgacgtcg gccagagatt tactcagcgc gcgcgctgca tcgtcaatgc
     2941 ccgtcaaccc gaccaggcgt gcattagcac cgagagaagc gatattcatc gccacgttag
     3001 ccgcgccgcc cggacgttct tcgatggtat tcactttaac cacgggcacc ggcgcttccg
     3061 gcgagatacg actggtgggg ccgtaccagt aacgatccag catcacatca ccaaccacca
     3121 tcactcctgc acgttcaaac tctggcagcg ttactttcat tcctgtctcc tgagagattc
     3181 aaaatttgcg cgcgataata ccatacttca ttcttccacc agccacttct gccagcttgc
     3241 ccgcaccaga tcacgctctg cggtgaagca atcctccgac acatggcccg gcaattcctg
     3301 taatgccaga tgatgaagtt catcgcgcag cgtcgtgtaa gcacgggtca gcgccatcgc
     3361 ttcctgctct tccataatgt cgttttgcgc cagcagctcc agaatacgca cgttgtctga
     3421 ccagcgcgtt aacttcggtt tttcatgagc gtagcgcaac accagatatt gggtaataaa
     3481 ttcgatatcg gtaattccgc cttcatcggc tttgatatca aagcgattac gatgtttatt
     3541 gccgagatga gcgcgcattt tctcgcgcat ttcccgcact tccgtttgca gagttttacc
     3601 ttctcgcggc agtgtcataa tctcacgacg caccgcgtca aagtgcgcag tgagctgcgg
     3661 atcgccgtac actacacgcg cacgcaccag cgcctgatgt tcccacgtcc aggcctcgtt
     3721 tttctgataa tcggcaaatg ctgctgcgga tgtcaccagc attcccgccg ccccggacgg
     3781 acgcagtcga gcatccactt catacaaaat gccggaagag gtacgcgtac tgaacagatg
     3841 cataatgcgt tgcgccagac gcagataaaa ctgccgcccg tcgatttccc gctcgccgtc
     3901 agtcatcgca tccattgggc aatcatggag gaagataagg tcaagatcgg aactgtagcc
     3961 taactcccag ccgcccagct tgccgtagcc gaccaccgca aaaccgcgcc cttcacgttc
     4021 gttgaggtga tttggcttac cgtagcgggc caccatttga ccccacgcct gctgaacgac
     4081 ggcatctatc atagcttctg ccagccaggt taagtgatcg ctcactttca ttaccggtag
     4141 cgtaccggcg atatccgctg ccgcaatgcg taacagctgc gcctgtttga actgacgcag
     4201 cgcctcaagc tgttgttctt cgtcatcttc cggcacgcgc agcaaatact ggcgcagttc
     4261 atcacggtag gcatcggtcg ccgtcggctg gtaaagggta tttggatcga gcaattcatc
     4321 cagcaataat ggataacgcg ctagctggct ggcaatcatc ggcgatgcgg cacacagaga
     4381 aatcaaatgt ttaagcgccg cagggaattc actgagcaat tctaaatagg ttgtgcgggt
     4441 aacaatcccc accagcaagg cggtaatgcg cgacagcgtg acagcagcgt cttcacgcgc
     4501 acagacatca cttagcagat gtggcatcag atggtcgagc acctgacgtc ctcgcggccc
     4561 gatagtacgc ttatccagct ctttgcggaa atcagcaatc agcgttagga cctgtttgcg
     4621 atcatcctcg ctaagatgcg ccagcactgg cgtagtgtca tcttcctgca acgcatcctg
     4681 ccacagctca cgccactgtt ccgacagcga ctcttcctga gtttcacttt cgtcgtcgcc
     4741 aatcaattca ttaaacaccc ggcgcacatt ggtcatatgt gcggtcagcg ccccggtcag
     4801 ttgcggccag tcagcaaagt ccatcgccca cgccagccgc gcacggttaa gctcatcaga
     4861 aggaagcgtt tgggtttgtt cgtcgttaat gctttgcagc aggttttcca gacgccgcag
     4921 gaacagatat gccactcgca attgttcagc atcgttttca gaaagcagat gcagcgcggc
     4981 aatggcgctg agcgttggca gtaaagagcg cgactgcagc aacggttcgc gcccaccgcg
     5041 aatgagctgg aacacctgaa cgataaattc aatttcgcga atgccgcctg cgccgagttt
     5101 gatattgtcg gtcaaaccac gtcgacgcac ttcacgggca atcatccctt tcatgttgcg
     5161 cagcgactga atcacgctga aatcgatgta acgacggaaa acaaacgggc gcagcatcgc
     5221 acgcaactcg ttagcataga cgccttcgct atcgcccata atccgcgctt tgaccatcgc
     5281 gtaacgctcc cagtcgcgtc cctgctcctg gtaataatcc tccaacgcgg caaagctcag
     5341 caccagcggg ccactttcgc caaacggacg cagtcgcata tccacgcgat agacgaagcc
     5401 atcctgcgtt ggttgatcca gcactttaat cagccgctgc cccatgcggg taaaaaactg
     5461 cgcgttatcc agttcccggc gtccgccctg cgtacaacca tgttccggcc aggcaaaaat
     5521 cagatcgata tcagaggaga aattcagttc cccgccgccc agcttaccca tgcctaagat
     5581 cagcagcggt tgcgcttcgc cctgcgcatt gcacggcgtt ccccactcgc ggcagcaggc
     5641 gtcatacagc cagtcacgcg ccgcaacaat cagcgtctcc gccagataac tgagctgctg
     5701 caatatgctc tcttcagtaa ccagtgccag cgtttgcgcc catgcgatgc gcaccataat
     5761 gcgccgccgg aatagccgca gctcgcgcat taacccggct tcgtcgctca cattactgag
     5821 cgcctcctgc aaccacacgc tgtaatgttg ccattcgtca gcctgtggcg gttggctttc
     5881 cagttccgtc agccactctg gatgcgcaat cacgctgtcc tgcacaaaat cactgaaagt
     5941 aagtactgac ttcgcctgtg cactaagtga ttcctcggct aaaagctctg gcagccgctc
     6001 aacaacggtc tgccagtact gctgtaacgg tgaagagagc ggcttcataa gcgattttat
     6061 ccttgcctga ttaacgtttt ccgctgtgca accagaacgg ttcctgattg tttgcctcat
     6121 tacggaaatg ttcaatttca atgcgttgcc cggtcgcaat agcgtgacgc agcccctgcc
     6181 agttctccag ccaggcttgc gcgacgacag gatcatagta acccgccagc aacagtattg
     6241 agtcaatatc acgcatcagg cgtggcaact ggtcacggta gcgatcgcct aacggctggc
     6301 agaaaacgct tttcagttca gcggcatggc gggaaagatg gatatcggca aagcgtttga
     6361 aggagtcgct aattttgccc tgcgctttgg catctaaaaa cggctgccat gctttgctta
     6421 ccagccattc ggtcaacgcc agcttcgcca ttgccgtttc ggtagagtag accgccgtca
     6481 cggcagaaac cgcagaagcg atggtcgctt cgcattgagt cagcaaatca cgtaagtgag
     6541 tgctcgcttt acgcggcaca atgccaccga acagcatcag tgtatgacgg actaggctaa
     6601 tggctgccag cacctgctct ttcgccgcat cgttgccgcg tacccacagt tcttcatgat
     6661 actgccattg cgctaacacc agctcgagcg ccgcttccag cccctgttcc acatcggctt
     6721 ttgccgcaac atgcaaaatg gtggtcggtt tgatttcacg cgccggattg ccctgcgcca
     6781 gatgatagcc acgcgccgct ttgctcaggc tgccctggcg taatccggtt tgcgatacca
     6841 gttggttcgc cagtttcagc accgcgcgcg tgtcgccgct aagcagctcc agttccagct
     6901 cgcagatagg ctcagcaaat tcacccgctt tcacttcccc ctggtcgaga gcgatttcaa
     6961 ttcggctgcc atcgatctct actagccatt tttcgcgata aaaatcggtg ctaaacagcg
     7021 gctgcacgcg ggaagcgaga tcggcaggca attcgccgtt cggccagatt tccgtcggta
     7081 actgcgccag gtcgagcgtc ggttcgctca acgccacgtt atattccggg cgctggtgta
     7141 agccgcctgt cactcttcct gcaactttca tggtcatctc atagcgaccg ttttcgccac
     7201 gaatacgtaa gcccatatcg tgcctacgca gccagttatc cggcgtttcg tagtaaatat
     7261 tcagcaactg cacggggtca tggtgctcgc cgcccagcgt attgagatgg tcacgcaacg
     7321 cctcaacggc actgtgatta acaataaact ttaattcgat ttcctgagcc atggccttta
     7381 cttatcggtt atgtcacata agtgacgatg aacggcgaac ttaatgcgat cttttttgtc
     7441 agtagatagt attttgcgcc aaattgccat gcaacgggca atttgacggg cgtaaaagtt
     7501 tgaagcagtg gcaagacctg acacagagga ttccgattga tgtcgaatgc tttgcgtagc
     7561 gacactgatg ccactactat cgttccactt tccatgacaa taacgacagc ctgatgccaa
     7621 aattacgcct gatcggatta actttactcg cacttagcgc gactgccgtc tcacacgctg
     7681 aagaaacgcg ctatgtttct gacgaactga atacctgggt ccgtagcggt ccgggagatc
     7741 attatcgcct cgtgggcacg gttaacgccg gcgaggaagt gaccttatta caaactgacg
     7801 ccaacaccaa ttatgcccag gtgaaagaca gctctggccg taccgcctgg atcccgttga
     7861 aacaacttag cactgagcca agcctgcgct cccgtgtgcc agatctggaa aatcaggtca
     7921 aaaccctgac cgataaactc accaatatcg ataacacctg gaatcagcgc accgcagaaa
     7981 tgcagcaaaa agtggcgcag agcgacagcg tgatcaacgg gttaaaagaa gaaaatcaga
     8041 aactgaaaaa cgagctgatt gtcgcgcaga aaaaggtcga tgccgccagc gtacagctgg
     8101 atgacaaaca gcgcaccatc atcatgcaat ggtttatgta tggcggtggc gtgctggggc
     8161 ttggcttgct gctcggtctg gtactgccgc acctgatccc aagccgcaaa cgcaaagatc
     8221 gctggatgaa ctaaatcgcc ttcttcgttg cactgtctta ttattaggaa ttgtgtattt
     8281 gacgaaggga tgatggcgtg aagatttatc tggtcggtgg tgctgttcgg gatgcattgt
     8341 tagggctatc ggtcaaagac agagattggg tggtggtcgg cagtacgcca caggagatgc
     8401 tcgacgcggg ctaccagcag gtaggccgcg attttcctgt gtttctgcat ccgcaaacgc
     8461 atgaagagta tgcgctggca cgtaccgaac ggaaatccgg ttccggttac accggtttta
     8521 cttgctatgc cgcaccggat gtcacgctgg aagatgatct taagcgtcgc gatctgacca
     8581 ttaatgcgct ggcccaggac gataacggcg agattatcga cccgtacaac ggtctgggcg
     8641 atctgcaaaa tcgtctgttg cgccatgttt cccccgcttt tggcgaagat ccgttacgcg
     8701 tactgcgcgt ggcgcgtttt gctgcgcgtt atgcccacct cggttttcgt attgccgatg
     8761 aaactctgac gctgatgcgc gagatgaccc atgcgggtga actggaacac ctgacgcctg
     8821 aacgggtatg gaaagagacg gaaagcgccc tcaccacccg caatccccag gtgttcttcc
     8881 aggtactgcg cgattgcggc gcacttcgcg ttttattccc ggaaattgac gcactgtttg
     8941 gcgttccggc acctgccaag tggcatccgg aaatcgacac gggtattcat accttaatga
     9001 cgctctcaat ggcggcgatg ctgagtccgc aggttgatgt ccgtttcgca actttatgcc
     9061 acgatctcgg taaaggactg acgccgccag aactttggcc gcgtcatcat gggcatggcc
     9121 cggcgggtgt taagttagtg gaacaactat gccagcgtct gcgcgtgcca aatgaaattc
     9181 gcgatttagc cagactggtg gctgagtttc acgatctcat ccacaccttc ccaatgctga
     9241 acccaaaaac catcgtcaaa ttatttgatt ccatcgatgc ctggcgtaaa ccgcagcgtg
     9301 tcgagcaact ggcgctgacc agcgaggctg acgtgcgcgg cagaaccggt tttgaatcag
     9361 cggactaccc gcaaggccgt tggttgcgcg aaggctggga agtggcgcag tcagtgccga
     9421 caaaagcggt cgttgaagcg ggatttaaag gcgtggagat tcgcgaggag ctgacccgac
     9481 ggcggattgc ggcggtagcc agctggaagg aacaacgttg cccaaagcct gactgagtat
     9541 aaaactaaac gttagcacga accgtcccct cgccccttcg gggagagggt tagggtgagg
     9601 ggaacagacc agcaccggtg cgaacaatca cccccacccc ggccctctcc ctacagagcg
     9661 agggagaaaa cccggcctgc tcccgaacct tttctcaatc aaaagaacac gacatacacc
     9721 gcagccgcca caataaagcg ataaatggcg aacgggataa acgaaatgcg cttaatcaat
     9781 tgcaggaagg ttttaatcgc tatcagcgcc accacaaaag cggtgataaa cccaacggca
     9841 aacatcggga tatcgccggt tgtcaggaag ccccagcttt tgtagagatc gagcgccgtt
     9901 gcgcccatca tcatcggtac cgccagcagg aacgagaact cggaagcagc gtaacggctc
     9961 acgcccatca gcatcccacc tgaaatggtc gccccggaac gggagaaacc cggccacagc
    10021 gccagacact ggaaacagcc aatcataaac gcctgacgat aggtcatatc atcaagaccc
    10081 ggcgcacgcg gctctttcgg cttcaggcat tcggcggcaa tcagcaacaa accgccaacg
    10141 accagcgcat acatcacatt tatcgggtta aacaatgact taatcgtgtc gtggaataat
    10201 agccccagta ccaccgccgg aatcatcccc agcaaaatgt ggatcagcgt taaacgacct
    10261 ttgctttcac cttcgtgctg caacgggcgg ccaaagtgga tgccaatcag gccaaacaga
    10321 cgccgccaga acatcactac taccgccaga attgatccta actggatcac aacttcaaag
    10381 gttttcgccg tgtcgccctc aaaccccaac aagtgaccga caataatcat atggcccgtg
    10441 ctggataccg gcagaaattc tgtcaatcct tcgaccacac ccaatattgc cgctatcagc
    10501 agcgagtgca tatcgctcat caataaaccc ctaaattatt aaaatgtacc gcttgtccga
    10561 actactgcag tatgaccagg ttataaccgt ttggtttaac agctgtaaaa ttaattattt
    10621 tctttcagat tattgccacg ctcaatgatt acgccaacat tcgccgcccg cgccactgcg
    10681 cctggcttgc tgagtttgat acgcacccac ggcgagttga agcgtgctaa cagcagctcc
    10741 gccacctctt cagccacgcg ttccaccagc gcaaaacgcg ccccctcgac gtggctgacc
    10801 accgtttctg caatgtcagc gtaactgagg caatccgcca catcatcact tttcgccgct
    10861 ttacggttat cccatgccat ttcgatatcg aacactaact tctgttcgat ggtctgttcc
    10921 cagtcgtaaa caccaatagt ggtgattacc gaaagttgct ctataaatac aatatccatc
    10981 acgtcctgcc tgctttttgg ctaaccggat accacttccg gcgaaatgtg cgtattatcc
    11041 acagattcat cgttgaacac gaattttcaa aacggaacag cttatgagtg caatcgcgcc
    11101 tggaatgatc ctcatcgcgt acctctgcgg ctccatttcc agtgccattc tggtttgccg
    11161 cttgtgtggg ctgcccgatc cgcgaaccag cggctccggc aatccaggcg caaccaatgt
    11221 gttacgtatc ggtggcaagg gagcagccgt agcagtactg attttcgacg ttctgaaagg
    11281 aatgttgcct gtctggggcg cgtatgaatt aggtgtcagc cccttctggc taggcttaat
    11341 tgccatcgcc gcctgtcttg gacacatctg gcccgttttc ttcggtttta aaggaggaaa
    11401 aggcgttgct accgcttttg gagcaatcgc accaattggc tgggatctca ccggagtaat
    11461 ggcgggaacc tggttactga ccgtgctatt gagcggatac tcgtcgctgg gagcgattgt
    11521 cagtgcactg attgctccgt tttatgtctg gtggtttaag ccacaattca ccttcccggt
    11581 ttcgatgctc tcttgcctga tcctgctgcg tcatcatgac aacatccagc gtctgtggcg
    11641 tcgtcaggag acaaaaatct ggacgaaatt caaaagaaag cgcgaaaagg atcccgagtg
    11701 atttctggtg gatctacatg acctgatagc cttcatcggg cttgcccagc cgttgctggc
    11761 accatgccgc cagaaattcc acgcagacac gaatttcatg ctgcgataga gcggctcccg
    11821 gtaaacagcc cagatattgg cgctttgtgc atactccggc aatacttgca ccagtttgcc
    11881 actctccaga aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag
    11941 cgcccattgc aacactatct cgccgctatt ggaggaaaga tgcccgctaa ccttcaccga
    12001 ttttttctcc tgaccgttcc ccaactccca tattccgtgg gtcatatcgc gctctttggt
    12061 caccagacag tcatgacgac ttaattcctg taatgattgt ggctgcggat atttttgcag
    12121 atactcaggt gctgcgcata atattctttt atttttcgtt aacagatgcg caatataata
    12181 atcaggaatt tcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat
    12241 ttgccgatcg aacaattcaa aatgcacctg taactcagga taattgcgca taagttcggt
    12301 aatggctggc gcaatatggc tgcgcccaaa accaaaactg caaccaatac gaatcatccc
    12361 ctccgggcgc gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat
    12421 ttccagcgca tgttcgtaac aacgttggcc actttcagtc agcgccacgc cgcgagcgga
    12481 gcggttcagc agcgttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa
    12541 tgcaggcgtt tgcccaagcg tcgccgctgc agcactaaaa ctcccgctgt ggacgatttc
    12601 taccagtacc tgcaagtctt tggctaaagg ccagctattc agcatcgtta attatccgca
    12661 gttgtgataa gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg gcgcaatatt
    12721 cattcttatt ttcgcgttga ttcacgaact gttaattaca tattcacggt ggcaaaaaat
    12781 ataaaaccac atttttagtg gtagtttgtg gcggtgaatt tttccagaca aatacaaaaa
    12841 ctggagttgc catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacagaga
    12901 ttgtcgctaa ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac
    12961 taaaacagct aaaggatgcc gaaacgtcgt cgatggggaa aattatctac catacgatgt
    13021 tcgacaacat gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac accggggaga
    13081 ttatgttctt cgttaaagtc ggttcccgct tcccactgct tggcgagctg caaagcatac
    13141 tcaaacaagc cgtggaagag gcaaccgtca aagcgccact acgtcacaat gcggtagaaa
    13201 tttttgacga agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg gtcacctggg
    13261 acatcatccc cgacaatgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca
    13321 cgctacctgg ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtggtgaaat
    13381 tcgtcttcga aaatatctcc accctcgccg taaacgcctg tccaccggta ctggtgggcg
    13441 tgggcatcgc cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc attctgcgcc
    13501 cgattggcag ccgccatccc aatccaaaag cggcagaact ggagctacgc ctggaagaag
    13561 gactcaaccg tctggggatt ggtccacaag ggctgaccgg caacagttca gtgatgggcg
    13621 catcgtcgcg gcacgctgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc
    13681 cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata
    13741 ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgc gacgtttgtc
    13801 accgccgttt gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt
    13861 tccacgctgg ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtc tccgtcggcc
    13921 cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tattgagcag accggcgtaa
    13981 aactggtggt tggcaaaggt ggtatggggc cgctgaccga agaaggctgc cagaaattca
    14041 aggcgttaca tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtagaag
    14101 agattgaaga agtgcactgg acagaactcg ggatgccgga gtcactgtgg gtctgccggg
    14161 tcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaac ctgatagccg
    14221 aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcatg
    14281 tccattacat caaataaccc tcccggagag gctcacccct ctcctttttc gcaggcataa
    14341 cacgatgaaa ccttccactg aatggtggcg atatcttgca ccgctggcgg tcatcgccat
    14401 tattgctcta cttccggttc ccgcagggct ggagaaccat acctggctct actttgccgt
    14461 ttttactggc gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat
    14521 ggtgggtatc tccatcatcg ccatactctc tccctggctg ctgttcagcc ccgaacagct
    14581 cgcccagcca ggctttaagt tcaccgccaa atccctctcc tgggcagttt ctggtttttc
    14641 taattcggtt atctggctca ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac
    14701 cgggcttgga cgccgcattg cgctgattct ggtgaaaaag atggggcatc gcacgctgtt
    14761 tctcggctat gcggtgatgt tctccgagct gatcctggca cctgtaacac cgtccaactc
    14821 ggcgcgtggt gcgggtatta tctatcccat catccgtaac ctgccaccgc tctatcaatc
    14881 acaaccaaac gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt
    14941 tgccgactgc gtgaccagcg ccattttcct gacggcgatg gcacctaact tgctgttaat
    15001 tggactgatg aaaagcgcat ctcacgccac gctgagttgg ggcgactggt tcctcgggat
    15061 gttgccgctc agcattttac tgcttctgct ggttccctgg ctggcttacg tgctataccc
    15121 gccggtactg aagtctggtg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat
    15181 gggcccgctc tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctgatgct
    15241 gcttctgcgc attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt
    15301 cttctggctg gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag
    15361 ctggtttggc aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt
    15421 gacgttgatt gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac
    15481 ctccgccctc gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc
    15541 ggtgttctgc ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc
    15601 caccggaccc agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg
    15661 actgggggcg atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg
    15721 gatgcccgtg gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat
    15781 agttttacgc agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat
    15841 ccgccgtcgc gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat
    15901 cagtacaaaa ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag
    15961 ccagcttcgc ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa
    16021 agcccgtctg atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt
    16081 caaaggcgcg ggcgatatca gcacgcgtct ggtcgtcggt gctgttgtca cgaatcgtgt
    16141 tcgccgcaaa ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg
    16201 gacgcgggaa aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc
    16261 cgccaggata atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat
    16321 catcgataga ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg
    16381 tatgaccgcc ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca
    16441 tcggcgctaa cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa
    16501 acgccagaga gcgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg
    16561 cggtataggc cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct
    16621 ggatcaacgg tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc
    16681 cgtagtcagc gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat
    16741 cgtcgtaaat ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga
    16801 cttgttttac ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact
    16861 ttgttcgccc ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa
    16921 aaaaggccgc ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga
    16981 aataagctgg cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc
    17041 ggtaattaaa gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc
    17101 ctgcgaaaaa gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac
    17161 taccgaacgt aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg
    17221 cgaaaacgca cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga
    17281 ccgagttgta gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg
    17341 aattgctaaa aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg
    17401 ctggcacgca ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc
    17461 aagaatttcc acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac
    17521 ggtgagaaac agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc
    17581 ctgatgaact acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac
    17641 aatcttgaag tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg
    17701 caaacgcttt atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa
    17761 cctgttgcca cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc
    17821 gctcgctttg cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc
    17881 ggcaatccag aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag
    17941 ggacgcagtt acgatcgttt ccgcgaacgg gtgatattcc ccattcgcga taaacgcggt
    18001 cgggtgattg gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg
    18061 ccggaaacag acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag
    18121 gataacgctg aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg
    18181 gcgcaatacg gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac
    18241 atacaactgt tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca
    18301 ggccgcgatg ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt
    18361 cagctacgct ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa
    18421 ggtaaagaag cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt
    18481 aacagtctga tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg
    18541 ctggcactgc cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag
    18601 gaattaggca acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa
    18661 gcggcagaga gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata
    18721 cttatagggt tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat
    18781 ctggatgaaa ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc
    18841 tcccagccag gtctgacctc cgggcaactt ttagagcact atcgtggtac aaataatgct
    18901 gccacccttg aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa
    18961 accttcaccg actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag
    19021 ttaatcgctc gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca
    19081 ttaaaccagg agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga
    19141 agcccccgac agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat
    19201 cgttggcggt aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc
    19261 gcccaacacc aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt
    19321 cacagctgaa acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg
    19381 tcaatgacca tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa
    19441 tgatcaacga catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc
    19501 tggctgaaaa caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg
    19561 tggaatctga aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca
    19621 ccgttgaact gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga
    19681 tcaaccaagt tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac
    19741 agtacgatcg tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg
    19801 acccgaacgc agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc
    19861 aggaagatct ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg
    19921 atgatgacaa cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc
    19981 agtacgttgt aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg
    20041 aagagatcct gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg
    20101 actacctggt caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga
    20161 tcatgaagct ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta
    20221 ccggcaacga aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt
    20281 cggaaaaact gcacgatgtc tctgaagaag tgcatcgcgc cctgcaaaaa ctgcagcaga
    20341 ttgaagaaga aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca
    20401 tcggtgaagc gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg
    20461 ttatttctat cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg
    20521 aaggtaacat cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt
    20581 tctccaccta cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg
    20641 cgcgcaccat ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt
    20701 ctcgccagat gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta
    20761 tgctgatgcc ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca
    20821 tggaaacgcc gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca
    20881 ccctcgagct gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg
    20941 tgctggctgg cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata
    21001 tgaacaccga ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta
    21061 tccgtcagat cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt
    21121 tgcgtagctt cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca
    21181 ctagtctctc tgcacaaacg ccaccttt
//
LOCUS       DAJTNM010000065        21018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTNM010000065 DAJTNM010000000
VERSION     DAJTNM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.065"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(1..2633)
                     /locus_tag="OO172_002456"
                     /pseudo
     CDS             complement(1..2633)
                     /locus_tag="OO172_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380005.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            complement(3093..3266)
                     /locus_tag="OO172_002457"
     CDS             complement(3093..3266)
                     /locus_tag="OO172_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512607.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            3384..4577
                     /locus_tag="OO172_002458"
     CDS             3384..4577
                     /locus_tag="OO172_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5512608.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4775..6028)
                     /gene="glyA"
                     /locus_tag="OO172_002459"
     CDS             complement(4775..6028)
                     /gene="glyA"
                     /locus_tag="OO172_002459"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5512609.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6355..7545
                     /gene="hmpA"
                     /locus_tag="OO172_002460"
     CDS             6355..7545
                     /gene="hmpA"
                     /locus_tag="OO172_002460"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5512610.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7590..7928)
                     /gene="glnB"
                     /locus_tag="OO172_002461"
     CDS             complement(7590..7928)
                     /gene="glnB"
                     /locus_tag="OO172_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5512611.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7989..9323)
                     /gene="glrR"
                     /locus_tag="OO172_002462"
     CDS             complement(7989..9323)
                     /gene="glrR"
                     /locus_tag="OO172_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5512612.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9313..10026)
                     /gene="qseG"
                     /locus_tag="OO172_002463"
     CDS             complement(9313..10026)
                     /gene="qseG"
                     /locus_tag="OO172_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5512613.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10191..11618)
                     /gene="qseE"
                     /locus_tag="OO172_002464"
     CDS             complement(10191..11618)
                     /gene="qseE"
                     /locus_tag="OO172_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5512614.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12176..16063)
                     /gene="purL"
                     /locus_tag="OO172_002465"
                     /gene_synonym="purI"
     CDS             complement(12176..16063)
                     /gene="purL"
                     /locus_tag="OO172_002465"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5512615.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16321..17877
                     /gene="mltF"
                     /locus_tag="OO172_002466"
     CDS             16321..17877
                     /gene="mltF"
                     /locus_tag="OO172_002466"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5512616.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17874..18410)
                     /gene="tadA"
                     /locus_tag="OO172_002467"
     CDS             complement(17874..18410)
                     /gene="tadA"
                     /locus_tag="OO172_002467"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5512617.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18435..19070)
                     /gene="yfhb"
                     /locus_tag="OO172_002468"
     CDS             complement(18435..19070)
                     /gene="yfhb"
                     /locus_tag="OO172_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5512618.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19279..20127
                     /locus_tag="OO172_002469"
     CDS             19279..20127
                     /locus_tag="OO172_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5512619.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20466..21018)
                     /gene="pinE"
                     /locus_tag="OO172_002470"
                     /pseudo
     CDS             complement(20466..21018)
                     /gene="pinE"
                     /locus_tag="OO172_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT         5048 a         5678 c         5351 g         4941 t
ORIGIN      
        1 gtagttaaaa ataaaaggca tcacagtggt gaataatgcc gctttcggcc aggccggatt
       61 aatcgccgtc aattcagcgc gagcagactc atattgcgca acctccccga gtcccgcgga
      121 cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat cgccatacag
      181 cgataacagg tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg ttttcgccat
      241 ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtttgct gttcacccag
      301 cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt tgtaataact
      361 gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgca tcgcttcagt
      421 ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac cagatgtcgt tatcagtttg
      481 ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat gaagcagttc
      541 gctggcctgc tgcggctctc tggcatcaag atgctgccag gcgcgtaata actggttgag
      601 gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt tacgcgtggc
      661 gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga gcagttctgc
      721 ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg ccagtcgttt
      781 ctccggtgtg gctccactta acttatccag caaatcccgt tcgaaaagca gacgtgcatc
      841 ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat aatcgtgttt
      901 cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg gcgacatctc
      961 tacgcaacgt tgccataagg caatggcttt gttgtagcta cgtttgttgt agtagaagca
     1021 ggccagtaaa tggcgagcaa accagcactc ttcaatactc tccagcgccg ccacttcttc
     1081 cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg cgaccagttc
     1141 gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg tgcgctggca
     1201 gtccagtgcg ttcagcatct ctgccgccag agtgggcatt ccccagttaa tcagttgccc
     1261 ggcggtcatc agagcgttaa cgtcgcgtcc ctgacacaga ccgcgccact gcgcgagggc
     1321 tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca gcggataatc
     1381 acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta acaccagcag
     1441 cagattatgc aagcaaagca cttcctgatt ggttgggcag gcacgaagac tttgttggca
     1501 aaaatccaga cccgcgtcga agttatcatt acgcgccgcc agtcgtgcca gaccataata
     1561 gccgcctgct ttactattgc cgctccagac cgcacgccag aaatcctctt cggcttgttg
     1621 atattgtccc tgacgttcgt aagcactggc gcgaatcaaa cttgcttgtc cacactgcgg
     1681 gtttttgttc agcgcatgtg cgcgtcctga ctggcatacg ccaccgcttg cgggaaatct
     1741 gcgcggttat attccagcat cgccagcgcc aggttacagc gataatccag cgggtccagc
     1801 gccacgccgc gcaggtagta atcgaacggt gaacggctgg cgtgatgata ttgctccaga
     1861 tgctgaccga taaaccaggc ttcatctgta ctggtaatgt cttgtgctgc cagtggcgct
     1921 ttggcgacgt ccggcaacgg caactcctgc ggctgatgtt cctgataact cagtacaata
     1981 ttgccgtcgg catcggagag ttcaatggtc agtctgtcag gattgatacc gtgcaacacg
     2041 ccctggatgg cggtcgctgg tgtcagggcc acggcatcat cgagcaacgc gttgcatttg
     2101 ccgatttcgc ggatcgccag acggtatccg ttcaacggag agatggcata cagcccccac
     2161 tcaatccccc gctcactacg ctggagtttt atcaccgcat cgcgggaggc attttgcacc
     2221 atgcacagag aatgataagg caggaaatac tgctcgaaac gcttctcttc gtaagcatca
     2281 agccaggtaa aatcaggctg gttatcggca aaaataccgg tcatcagttc gatatacggg
     2341 ccgttattgt cggtcagact cttatcccac gcctggccaa attcactgtg tccccaactc
     2401 cactgttttt tacctggcgc aatatggtgg ttggcaacgt gcagcaaacc gccatcttca
     2461 tcgtgacacc acgcgccgac aaaatcgtac tgcgattttt cggccatata tgaggttgga
     2521 acaggcacat ttttatagcg agaaatgtcc actccggcgg agtagtccac tttgtagtaa
     2581 gtgccggtgg cgatggggaa agcggagacg gcccgtttgc cgtgatcaaa caccgcagtc
     2641 acatccggtg ggaaaacgct ctgatgccct tcaccccctt tcactgccgg gttggcccac
     2701 cacaagaaat gacgcggcgt ggcgttgccg ttatagacgc ggctggcgat ttccagtgcc
     2761 gcccggtcag ggcgcagggt gaaacctgtc atcacctgta aaccatgcat cggctccgtt
     2821 tcgccgaccc acaccgtctg tgcaccgtct tcatgggctt cgagggtgaa atcaacgggc
     2881 ataaaggtgg tcgggcgatg gtgttgcggc cagttaaact caatcccgcc ggagatccac
     2941 ggtcccagca gccccaccag cgcaggttta atgacttcat tgtgatagac aaaatcgcgc
     3001 tgtttcactt tatcccatgc gcgatgcacc cgaccgccca gttccggcag gatcatcact
     3061 ttgatgtagt cgttttccag ccacaccgcc tgctaggatt tcagggtttt ctgctcgctc
     3121 agcgtatcgg tcacgccgta gggataaacc gcgccggacg atccctgata aacgcgattt
     3181 tccaggaaca tgggatgtat atcctgcggc ccggtttcat aggtcgggat ctcaacgcgc
     3241 tcttgccaca cttttactgg agtcatggtg ccctcaatat taacaagaca tactgaatta
     3301 aaagatttgt ggcagtgtat tgaacaatct ggcaatgttt tcgcggaata atcacgcaat
     3361 taactaaaca aggtttagtg aagatgagag cctgcattaa caatcaacag attcgccacc
     3421 ataacaaatg cgtgattctg gaactgctgt accggcaaaa gcgcgccaat aaatcaacgc
     3481 tggcccggct ggcgcaaatt tcgattccgg cagtcagtaa tattttgcag gaactggaaa
     3541 gcgaaaaacg ggtggtgaat attgacgatg aaagccagac gcgcgggcat agtagcggta
     3601 catggctgat tgcgccggaa ggtgactgga cgctgtgcct gaacgtgacg cccaccagta
     3661 ttgagtgtca ggttgctaat gcttgtttaa gtccgaaagg cgaatttgag tatttacaga
     3721 ttgatgtacc gacgccgcag gcgctgctgt ccgaaatcga aaaatgctgg catcgccacc
     3781 gtaaattgtg gccggaccgc accatcaatc tggcgctggc aatccacggg caggttgatc
     3841 cggtgaccgg cgtatcgcaa accatgccgc aagcgccgtg gacaacgcca gttgaggtga
     3901 agtatctgct ggaagagaag ctgggcattc gggtgctggt cgataatgac tgcgtgatgc
     3961 tggcgcaggc ggagaaatgg caaaataatt cgcaggaacg ggatttctgc gtgatcaacg
     4021 ttgattacgg cattggctcg tcgttcgtga ttaacgagca aatttatcgc ggcagtttgt
     4081 atggtagcgg acagattggt cacaccatcg ttaatccgga tggcgtcgtc tgcgactgtg
     4141 gacgttacgg ctgcctggaa accgtcgcct cgttaagtgc attaaaaaaa caggcgcggg
     4201 tatggctaaa atcacaaccg gttaatactc agcttgatcc tgaaaaactg acaaccgcgc
     4261 agttaatcgc tgcctggcag agtggagaac cgtggatcac cagctgggtt gatcgctctg
     4321 ccaatgccat tggtttgagt ctgtataact tcctcaacat cctcaatatt aatcagattt
     4381 ggttgtacgg tcgcagttgt gcctttggtg agaactggct taatactatt attcgccaga
     4441 caggatttaa cccgttcgac cgcgacgaag gaccgagcgt gaaagcgacg caaattggct
     4501 tcgggcaatt aagccgcgca caacaggtgc tgggaattgg ctatttgtat gttgaggcgc
     4561 agttacgaca gatttgatgg cgcgataacg tagaaaggct tcccgaagga agccttgatg
     4621 atcataaacg aaaaattgcc tgatgcgcta cgcttatcag gcctacacgg agattgcaat
     4681 atattgaatt tgcaagattt tgtaggcctg agaaggtgtt catgccgcat ccaacatgaa
     4741 caacgagcac attgtcagca aatcaccgtt tcgcttatgc gtaaaccggg taacgtgcgc
     4801 agatgtcgag aactttacct ttgatgcgct cgataacggc ttcatcattg atgctgtcca
     4861 gcacgtcaca catccagcca gccagttctt tcgcttcagc ttctttaaag ccgcgacggg
     4921 taatcgccgg agtacctaca cgaataccgg aggtcacaaa cgggctcttc ggatcgttcg
     4981 gtacgctgtt tttgttgacg gtgatgttag cacggcccag agcggcgtct gcttctttac
     5041 cggtcaggtt tttatcaacc agatcaacca ggaacaggtg gttatcagtg ccgccggaaa
     5101 ccactttgta gccgcgctcg aggaacactt ctaccatcgc tttagcgttt ttcgcgacct
     5161 gctgctggta agttttgaac tcaggctcca tcgcttcttt cagagcaacc gctttaccgg
     5221 cgattacgtg catcaacgga ccgccctgac caccagggaa aacggcagag ttcagttttt
     5281 tgtacagctc ttcgctacca cctttcgcca ggatcaggcc gccgcgcgga cccgccaggg
     5341 ttttgtgagt ggtggtagta acaacgtgag catgaggaac cgggttcggg tagacgccag
     5401 cagcaaccag gcccgcaacg tgcgccatat caacgaacag ataagcaccg atgctgtcag
     5461 cgatttcacg cattttcgcc cagtcaacca cgccggaata tgcagagaaa ccaccgataa
     5521 tcattttcgg cttgtgttct ttggcttgtt tttccagatc ggcgtaatcg atatgaccgg
     5581 tagcatcgat accgtaagga acgatgttgt acagtttacc ggagaagtta accggagaac
     5641 cgtgagtcag gtgaccgcca tgcgccaggt tcatacccag aacggtatca cctggttcca
     5701 gcagcgcggt gtagaccgca aagttagcct gggagccgga gtgcggctgg acgttagcgt
     5761 agtcagcgcc gaacagttct ttcgcacgat cgatcgccag ttgttcaacg atatcaacat
     5821 actcgcaacc gccgtagtag cgtttgcccg gataaccttc agcatatttg ttggtcagct
     5881 gagaaccctg cgcctgcatt acgcgcgggc tggtgtagtt ttcggaggcg atcagttcga
     5941 tgtgctcttc ctgacgtact ttttcctgct ccatagcctg ccacagttcg gcatcataat
     6001 cggcaatgtt catttcacgc tttaacatcc gcatctcctg actcagctaa caataaaatt
     6061 tttggccttt ataggcggtc ctgttggaca acggcgaaca gtataaccga atcattgtgc
     6121 gataacaggt cttgacaaag gaatttacgc aaacgattac cttaaggcta cgcaaggctt
     6181 tggagaataa agagcttgca accggaaacg gatttctttt caggtttgtg atgcaaattt
     6241 ttcacttcat cacattcttt ctgaaaaaca ccaaagaacc atttacattg cagggctatt
     6301 ttttataaga tgcatttgag atacatcaat taagatgcaa aaaaggaaga ccatatgctt
     6361 gacgctcaaa ccatcgctac agtaaaagcc accatccctt tactggtgga aacggggcca
     6421 aagttaaccg cccatttcta cgaccgtatg tttactcata acccagaact caaagaaatt
     6481 tttaacatga gtaaccagcg taatggcgat caacgtgaag ccctgtttaa cgctattgcc
     6541 gcctacgcca gtaatattga aaacctgcct gcgctgctgc cagcggtaga aaaaatcgcg
     6601 cagaagcaca ccagcttcca gatcaaaccg gaacagtaca acatcgtcgg tgaacacctg
     6661 ttggcaacgc tggacgaaat gttcagcccg gggcaggaag tgctggacgc gtggggtaaa
     6721 gcctatggtg tactggctaa tgtatttatc aatcgcgagg cggaaatcta taacgaaaac
     6781 gccagcaaag ccggtggttg ggaaggtact cgcgatttcc gcattgtggc taaaacaccg
     6841 cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg acggtggcgc agtggcagaa
     6901 taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg aaggtttccc gcatcaggaa
     6961 attcgtcagt actctttgac tcgcaaaccg gatggcaaag gctatcgtat cgcggtgaaa
     7021 cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc acgccaatgt tggcgatgtc
     7081 gtgaaactgg tcgctccagc aggtgatttc tttatggctg tcgcagatga cacaccagtg
     7141 acgttaatct ctaccggtgt tggtcaaacg ccaatgctgg caatgctcga cacgctggca
     7201 aaagcaggcc acacagcaca agtgaactgg ttccatgcgg cagaaaatgg cgaagttcac
     7261 gcctttgctg atgaagttaa ggaactgggg cagtcactgc cgcgctttac cgcgcacacc
     7321 tggtatcgtc agccgagcga agccgatcgc gctaaaggtc agtttgatag cgaaggtctg
     7381 atggatttga gcaaactgga aggtgcgttc agcgatccga caatgcagtt ctatctctgc
     7441 ggcccggttg gcttcatgca gtttaccgcg aaacagttag tggatctggg cgtgaagcag
     7501 gaaaacattc attacgaatg ctttggcccg cataaggtgc tgtaatttga tgttgccgga
     7561 tggaaacatc cggcaaccct tgacgcggtt taaattgccg cgtcgtcctc ttcaccggta
     7621 cggatgcgaa tgacccgagc cacgtcaaag acgaagattt taccgtcacc gattttgccg
     7681 gtttgcgccg tgcgaataat ggtatcgaca caggtatcga caatgtcgtc cggtacgaca
     7741 atctcaattt tcactttcgg cagaaaatcc accatatact ccgcgccgcg gtacagctcg
     7801 gtatggcctt tctggcgacc aaagcctttc acttcggtca ccgtcatgcc ggtaataccg
     7861 acttcggcca gtgcttcgcg gacatcgtcc agcttgaagg gttttataat cgcatcaatc
     7921 tttttcatgc tattccttga aaaggttgtc tgtcttttga tctgctaaac gtaacacata
     7981 acgccaattc attccttgaa atcgtttgca tccagctcgt gtcgggaaag cagtttataa
     8041 aattctgtcc ggttgcgccc cgccattctc gccgcgtggg tgacgttgcc tttggtgatt
     8101 tgcagcagct tacgcaaata gttgagttca aaatgattac gtgcctcaac aaaggttggc
     8161 agcgccgtat tttcaccctc cagcgcctgc tccaccagcg catcactaat caccggagat
     8221 gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact ggcgcacatt acccggccag
     8281 ctcgcggtca tcaggcgttt catcgcatcg gtagagaacg cgcggacaaa cggtttatgt
     8341 cgctctgccg cctggcgcaa caggtgattt gccagtagcg gaatgtcttc tgtgcgctcc
     8401 gccagtgccg gaattttcag gctgacaacg ttgaggcggt aatagagatc ttcgcggaat
     8461 tccccgcgcg ccatcgcttt tgacagatca cggtgagtgg cagaaataat ccgcacattg
     8521 atatcaatat cgcggttact gcccagcggg cgcactttac gctcctgcaa cacgcgcagc
     8581 agtttgacct gtaacggcgc aggcatatcg ccaatttcat cgagaaatag cgttccgcct
     8641 tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc cagtaaacgc gccacgcgca
     8701 tgaccaaaca gctccgactc cagcaattgc tcgggcaatg cgccacagtt aatagcaata
     8761 aatggtttgc tgttgcgcgg gctggcgttg tggatagcct gggcgaaaat ctctttcccg
     8821 gtgccgctct gaccgttaat caaaacgctg acgtctgatt gcgccaccag ccgcgcctgt
     8881 tccagcaaac gcagcatcag cgggctgcgg gtgacaattg cctcgcgcca gcgttcatcg
     8941 gtggctggcg cggattgctc cagcgcatcg tcaattgcct gatatagcgc atctttatcg
     9001 acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg cagcaacggc atcggggata
     9061 gaaccatgcg cagtaagaat aattactggc attcccggct gcactttctg gatttcagca
     9121 aatagctgca taccgtccat ttcatccatc cgcaggtcgc tgataactaa atctactttt
     9181 tcgcgattca gtacccgtaa tccttcagcg ccactttccg ccgtgaccac actgtagcct
     9241 tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg gatcgtcatc gaccaataat
     9301 aaatgcgcag gtttatggct catcaggagt gacctcatgg gtggatggcg cgggcttttc
     9361 actctcgtgc ggcgtatccg gcgagaaatt tccagccggt ttgcgggtcg agagctggcg
     9421 ttcaatatcg gtcaggtttt ccagcttgcg ggtggtgagt tccagttgct gctgtagaac
     9481 gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg gactgctgga gtttactgta
     9541 acgttggcgc tcttccgcca gttgcagttg cagcgcctga ccgtcgcgcc agagttgata
     9601 cagtgggcga acctgtgccg ggatctcggt acttaacgct tcaatacgcg cgaccagctg
     9661 gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa atcccttgtt taaaggtatt
     9721 ttgccagctg ccgtcgtcat attggcgagc ttgctgacgc gactgcgcag gcattaaacg
     9781 atcagcacaa tccatcgccc gcagccagta aagcggattg gtttcggttg atttaccttg
     9841 cagcgcccag atgtcgctac attcagtaga aagatagtca gccagttgat aaaccggaat
     9901 tttttcttct gctggcgtat caatggctgg cttattgtga ttctgcacgc aacccagcaa
     9961 tgccagacat ggcagccctg ccagccacag ccgtcggggc aataatcgtt gaaaaatgtg
    10021 tcgcatattc accagactta aagcctatcc cagtgggcgt aattgttgca gacagtctgg
    10081 acatggacag cgcggagaaa ccggggcgta catatcgtac gtgaagattt cgagcactac
    10141 ccggggccga actgacaaat aaaatagcct gataggatag gctgttagca ttatttcgtg
    10201 tttttcgacg acggtaattc aatgcggaaa caaacgtctt gcccgctctc gtcgaccaga
    10261 tacagttccc cttgcatacg gcgaatacaa tccctggcaa tgcttaatcc cagaccgctg
    10321 cccttcaccg ccccttttcg ctggtggctt ccctgaaaaa agggttcgaa gatcatggcg
    10381 cgttcctctt gcggaatggg cgtgcctgta ttgatgacat caatataaac ccgcgcacca
    10441 tgtaaactgc tgcgaaggca aatgttaccg gattcagccc cgtagtgcac cgcattggag
    10501 taaagattat ccagtacgct catcagcagc attggctccg ccaagcaagc tgttgctttg
    10561 agatcgacgt cggtatgcat cattttagcc cgtgcgggca ggctatgagc agaaaccact
    10621 gtctccacca gcggtgctaa ttcaacattc tccagttcca ccgcactgtc cgcctgttta
    10681 cggttgtaat caagcagttg ttcgatcagt ttttgcaaat tgcagctgct gctatcaaga
    10741 atgctcacca cctctttttg ctctggcgta agcggcccga caacctgatc agccagtaat
    10801 tcagtgccct cgcgcatact cgccagtggc gtttttaatt catgagataa atgtcttaaa
    10861 aattgatggc gttgggattc cagccatgac aggcgctcac ttaaccaaag aatacgttgc
    10921 ccaaccgagc gtaactcgct cggtccacag aacgaaacgc tattgcccag agaacgccct
    10981 tcccccagac ggttgatcat gcgctcgata tttttcaccg gcccgataat catccgcgtg
    11041 aaaagtagca ccatcaccag actcaccaga aatagcacca gcgattgcca accaaaatat
    11101 tgcccacgtt cggcgatttc acgctgaagt tgctgcccac gagagaacac cactgtgcgc
    11161 gtggcctgca ccatttcggt attggcactg gcaaaggctt caagacgcgc ggcggcggca
    11221 gcatcgggac cactgttgtt acactgaagt tgggccagat tgttcaaatc ctgacgcaat
    11281 gcctggtaaa gtttatcgtc tggcagtacg cctgcgtggg catcgagcat ttcgctgtaa
    11341 cgcttgcgct ggctttgata aaccttcgca agcgtagggt cgtccagcac gcaatactga
    11401 cggtagctac gctccatctc cagcgccgcg ttggtcatcg cttcactacg ccgggcatca
    11461 ataagcgtag tgcggttaac cagcgccgcc tgatcgctaa gcgcattcag gctttgccag
    11521 gcttgccatg ccagcaccaa caggggtagt agaattagca aaaatgccag cattaccagt
    11581 tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg tgttactctc gtcagacgcg
    11641 aatagcctga tgctaaccga ggggaagttc agatacaaca aagccgggaa ttacccggct
    11701 ttgttatgga ataaggcggt gcctaactcg acgtttcgcc cgatggttga tatagctacg
    11761 ctgatatcag aagttggacg gcaggcacct tgttgtgcgt cattcgaatt ttatgtagca
    11821 cgtcccgaag gggctgacat aagtcggtga atgagccact gcttactatt atgcagtaac
    11881 tgtgccaata aagaaaatag tttggtaacg tactgattat acgttgcttt gagagattta
    11941 tggcttcttc gctatttgaa tgtaaatcag ccactgtgtc gctaaaaaga gacaacttaa
    12001 gataaactta ttagattaca ataaaatcaa tcatttaagt gtcgccagaa agcgacacgg
    12061 taaaccaccc attgtcgtga tttacagaca caaaaaagct cccagagttg ggagcttatg
    12121 atagtggttg gtgcttaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    12181 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    12241 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    12301 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    12361 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    12421 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    12481 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    12541 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    12601 gccacaactc gctccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    12661 atacccccag ggccagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    12721 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    12781 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    12841 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    12901 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    12961 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    13021 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    13081 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    13141 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    13201 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    13261 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    13321 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    13381 ccagcgttac cagcaggccg ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    13441 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    13501 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    13561 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    13621 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    13681 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    13741 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    13801 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    13861 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    13921 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    13981 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    14041 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    14101 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    14161 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    14221 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    14281 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    14341 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    14401 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    14461 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    14521 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    14581 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    14641 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    14701 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    14761 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    14821 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    14881 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    14941 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc ggtaacaatg cgctcaggct
    15001 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    15061 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    15121 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    15181 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    15241 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    15301 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    15361 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    15421 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    15481 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaggcca agacgcaggt
    15541 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    15601 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    15661 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    15721 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    15781 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    15841 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    15901 gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc agcaaaatgg acatactcgg
    15961 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    16021 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    16081 agctttcggt gtacccaagg gggggaaacg ggcgtcatta taaagaatct gatgcgctga
    16141 cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt gccgccaatg
    16201 gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt tatgagtaaa
    16261 cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag aattaactaa
    16321 ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct gctcgcggtc
    16381 gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc cgccattcaa
    16441 gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa cgaaatcaac
    16501 gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta cctcggcgta
    16561 aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct tgataatggt
    16621 aacgccgacc tgctggcggc aggactggtc tataacagtg agcgggtaaa aaattatcag
    16681 cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtggg tcagtatcgc
    16741 ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg tcatgtggtg
    16801 gttaacgatc tccagaccct gaaagaaaca aaattcccgg aattaagctg gaaggtagac
    16861 gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact cgattacacc
    16921 attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct cgccgtagcg
    16981 ctcgatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg cgataatacc
    17041 ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac gctggcacgc
    17101 attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac gcgcacattt
    17161 ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa atacgccgaa
    17221 gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg ggatgcacag
    17281 gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac cgcgcaaagc
    17341 ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt gcgttatttg
    17401 caggatatga tgagtaaagt gccggaaagt gtgccggaga acgaacggat ctggtttgcc
    17461 ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct gacggcaaaa
    17521 accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt acttagccag
    17581 aaaccctatt acagcaagct gacttacggc tacgctcgtg ggcatgaagc ctacgcttat
    17641 gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga gaaagagaag
    17701 caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc gcctacggag
    17761 ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc aaactatttg
    17821 acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca aaattaatcc
    17881 gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg aaagaagtca
    17941 ctgagcaacg ccgcgcactc atccgccagt attccttccg taatttccac tcggtgattc
    18001 atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt cgcgtcacgc
    18061 gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca cattacacat
    18121 ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat caccagacca
    18181 ccctgccgca gggccatgat ttctgcatgt gcggtgggat catggcgacc aatcgggcgg
    18241 ttccagcctt cgccgattac ccgattgtta tgcactaata ccgcgccgac cggcacttcc
    18301 cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac gcatccagta ttcgtggcta
    18361 aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat tatacacgga
    18421 cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac tcgccaacga
    18481 tgctgacaga aataaagcag cggattgtcc tgattactgt cgctatagcc actgtacagc
    18541 cgcagcggcg tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc cagacaacgc
    18601 atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag attaacccgc
    18661 ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga gccggtaatc
    18721 aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg aaccagcgga
    18781 aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt ctgtaaacgt
    18841 gcttcgctgt gaccaaaagt gcacccccac agaagcagac tcatcggcca gcgtgccgca
    18901 cgacctttta tcaataacgc aatggctata atcggtaaca acggcaggac aagtaacgca
    18961 ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc cgaacatatc ctgctgatgc
    19021 aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa accatactcc
    19081 tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga tagatcatca
    19141 cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc atggcaatta
    19201 gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg tcgccactat
    19261 ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta ccaggggctt
    19321 gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac ggcgcgccat
    19381 ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt cgtgaagttc
    19441 gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag tgaagcgctg
    19501 gcaagccagc cggaatcgcc ctccgtgccc attcataacc aaatccgcgg tgatgatccg
    19561 ttacggctgg tcggcgaaaa actgattaaa gaaaatactg ccgctatgta cgcaacgcta
    19621 aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc tgcgcggcgg
    19681 ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc ctggaagctg
    19741 atgaagattg gcttcaatgc tgccgcactg cgcgatatgc atgcgctact cgcaacagta
    19801 caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt acgacgcgag
    19861 ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc gattaccggc
    19921 tttactccga atgccctgca acagcgtgct tctcattgcc tgtataccat tgccgaagaa
    19981 caagcgacaa acagtgcttc aatctctgct tgtcacgctc agggaatgtt aacggatttg
    20041 ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat tcgtcatagt
    20101 gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc cgcccggttt
    20161 gtgttgtttt gagagtttcc ttatggcgct gttaatcact aaaaaatgca tcaattgtga
    20221 tatgtgtgaa atcaaataaa aacacattta ttttcatagt gatacagcaa tgtgttattg
    20281 aatgtgttgt tggatttatg gtgcaaaaaa attttgcata agttcagcaa cacaaagaac
    20341 accactttct ttaacgtcct ccgcgcccta aaggacgggg ctttacggcg caaaaggcct
    20401 gtgacataca acattaactt ttcagtcaac cgtagcacgt ccttgtatac gccgtgtctg
    20461 cggttttata taaccctggc tggaaacctc ttatacaaag tcgattcgcc aacatcatag
    20521 atgatcgcca ccttctggcg agaaactcct gatgcaatta gtcgcccggc ctgcgcccat
    20581 tattctggtg aaagtttggg acggcgaccg ccaattcgtc cctgtgcgcg agcagcttcc
    20641 agtcctgctt ttgttcgttc aacaatcagt tcacgttcca tttcagccag ggcacccatc
    20701 acatggaaga aaaagcgtcc cattggtgtg ctggtatcaa ttgaatcagt cagactacga
    20761 aagttgatgc ctcgttcgcg caactcctcc accagcacga caagatgccg catactgcgc
    20821 cccagccgat ccagcttcca gacaaccagt gtgtcgcctg ccgataatgt cctgagcagt
    20881 tttttcagcc ccggtctttc ggactttgta ccacttatct tgtcttcaaa aatcagctcg
    20941 catcctgcac agttcaacgc attacgttgt agatcggtat tctggtcatt tgttgatacg
    21001 cgtacatagc caataagc
//
LOCUS       DAJTNM010000066        20718 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTNM010000066 DAJTNM010000000
VERSION     DAJTNM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.066"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..491)
                     /locus_tag="OO172_002471"
     CDS             complement(<1..491)
                     /locus_tag="OO172_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512620.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSL"
     gene            complement(499..1209)
                     /locus_tag="OO172_002472"
     CDS             complement(499..1209)
                     /locus_tag="OO172_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5512621.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1425..3248)
                     /gene="typA"
                     /locus_tag="OO172_002473"
     CDS             complement(1425..3248)
                     /gene="typA"
                     /locus_tag="OO172_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5512622.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            3621..5030
                     /gene="glnA"
                     /locus_tag="OO172_002474"
     CDS             3621..5030
                     /gene="glnA"
                     /locus_tag="OO172_002474"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5512623.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5203..6252
                     /gene="glnL"
                     /locus_tag="OO172_002475"
     CDS             5203..6252
                     /gene="glnL"
                     /locus_tag="OO172_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5512624.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6264..7673
                     /gene="glnG"
                     /locus_tag="OO172_002476"
     CDS             6264..7673
                     /gene="glnG"
                     /locus_tag="OO172_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5512625.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            7785..7895
                     /locus_tag="OO172_002477"
     CDS             7785..7895
                     /locus_tag="OO172_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5512626.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8124..9497)
                     /gene="hemN"
                     /locus_tag="OO172_002478"
     CDS             complement(8124..9497)
                     /gene="hemN"
                     /locus_tag="OO172_002478"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5512627.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(9686..10195)
                     /gene="yihI"
                     /locus_tag="OO172_002479"
     CDS             complement(9686..10195)
                     /gene="yihI"
                     /locus_tag="OO172_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5512628.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            10777..11409
                     /locus_tag="OO172_002480"
     CDS             10777..11409
                     /locus_tag="OO172_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5512629.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(11791..14577)
                     /gene="polA"
                     /locus_tag="OO172_002481"
     CDS             complement(11791..14577)
                     /gene="polA"
                     /locus_tag="OO172_002481"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5512630.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYTEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            14941..15873
                     /locus_tag="OO172_002482"
     CDS             14941..15873
                     /locus_tag="OO172_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5512631.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(15914..17344)
                     /locus_tag="OO172_002483"
                     /pseudo
     CDS             complement(15914..17344)
                     /locus_tag="OO172_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
     gene            complement(17499..18125)
                     /gene="dsbA"
                     /locus_tag="OO172_002484"
     CDS             complement(17499..18125)
                     /gene="dsbA"
                     /locus_tag="OO172_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5512632.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18142..19128)
                     /gene="srkA"
                     /locus_tag="OO172_002485"
     CDS             complement(18142..19128)
                     /gene="srkA"
                     /locus_tag="OO172_002485"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5512633.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRQTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19205..19474)
                     /locus_tag="OO172_002486"
     CDS             complement(19205..19474)
                     /locus_tag="OO172_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5512634.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            19544..20128
                     /gene="mobA"
                     /locus_tag="OO172_002487"
     CDS             19544..20128
                     /gene="mobA"
                     /locus_tag="OO172_002487"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5512635.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20110..20637
                     /gene="mobB"
                     /locus_tag="OO172_002488"
     CDS             20110..20637
                     /gene="mobB"
                     /locus_tag="OO172_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5512636.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5047 a         5232 c         5011 g         5428 t
ORIGIN      
        1 agcgacacca gctcatgact ttttaaatct tcatgctgct caatcgtaaa agtcaggccg
       61 ctgtcctgat acgtttcccg tacataggca atgtcgttag cgataatcga taatacgtcg
      121 cgcaaattac cttcataacg tggataaaaa cgtacggacg ttgccccagt tttcaacgcg
      181 atggcgacgg cttcgtcaat agatgcctta tctgaagcgc tggtttcaat atgaatatca
      241 aggttcagac ggcgggcttt atccccaaag gcagagagtt ctttatcgtc catattacca
      301 agagaaaaac gctcgccatc cagaacatgg attttgacgc cacgtagatt attttcactt
      361 gcgatatcaa gtaaatcagc aggcaacact ctttccagcc gcatatttaa atgaaaggca
      421 taagcatgta aataaagagg aagagtgtcg acacgttgta gaatctttct tgcgttattt
      481 attgtcacca taaaacacct acagattaat gatgaggtct acggcctcaa atcgccaata
      541 ttcaatatct atctggacaa tattgccttg ctggtcggcg cgatgctttt caattaagat
      601 cgctggcata cccgcagaac cgccaagata cttgctgata tcgcccggca ttctgacggg
      661 tttgaaaatc aattttttta ctaccgtctc tttttggtag cacttttccc agactgcaga
      721 aaatgagtgg ttttcaagtt gttcaataaa acccggagca acttcaggat taatatatgt
      781 ttcgtgatag aaaactttat gtccttccag cgccccccag ccggtgatgc gatagagttc
      841 agttgatggc gtcactgcaa tcagtggcgc gagcgtctcg ggaatatcgc tggtacggtt
      901 tttctcggta aacccccaag aaggctctcg cccttgctca attgcggccc gctgaaagct
      961 cgccgacagc tccggactgt aattaaatcg cggctgggtc acgaaccacc ccttacgatc
     1021 cttacggaag attttggatt ctgcctcgag gttcaacaac gcctggcgca gcgtcatacg
     1081 tttaatgccc agcagttctc ccagctctct ttctgaaggg agttttccac ccggcgtggt
     1141 gatgccatct ttcaaccacc gatccagttt ctcttttgca ttttctacgg tggattgatt
     1201 ctccgccatt gtttaatcct tttggtttaa accagatgaa gcatcttacg cttacgccaa
     1261 aaaagaaagc aattaacggt gtatgacttt gcagataatt gtgacaaagc tctctattga
     1321 cgtaatcaca gacttataag ggagatactc gcattggtgt gcgctcacga caggtaaaaa
     1381 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
     1441 gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
     1501 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
     1561 atgcggatag gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc
     1621 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
     1681 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga
     1741 ccgaacagcg cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga
     1801 cgctgaccca cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga
     1861 ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
     1921 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
     1981 ttacgttcgc ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc
     2041 acgttttcat acggctcttg tttacgaccg tcgatttcac ggaagattac tttcggacgg
     2101 gataccgcca gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt
     2161 tcaccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
     2221 acgttgtgta ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta
     2281 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
     2341 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
     2401 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
     2461 atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct
     2521 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca
     2581 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
     2641 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc
     2701 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
     2761 acgatcggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
     2821 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
     2881 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
     2941 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
     3001 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
     3061 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
     3121 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
     3181 tctaccaagg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
     3241 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
     3301 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
     3361 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
     3421 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
     3481 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta
     3541 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
     3601 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
     3661 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta
     3721 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
     3781 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
     3841 ccgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
     3901 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
     3961 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
     4021 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
     4081 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
     4141 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
     4201 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
     4261 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
     4321 acgaaattca gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg
     4381 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
     4441 ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
     4501 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
     4561 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg
     4621 cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
     4681 cacgtcgtat cgaagtacgt ttcccggatc cggcagctaa cccgtacctg tgctttgctg
     4741 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg
     4801 acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct
     4861 ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg
     4921 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
     4981 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
     5041 tgtactaccc ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct
     5101 gcatgggctt ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct
     5161 ataatgcact aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag
     5221 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat
     5281 ctggcgatcc attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa
     5341 ttgtttggta cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg
     5401 caagaaagtc tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat
     5461 ggtcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg
     5521 gagatggcac cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa
     5581 caagtagctg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc
     5641 ggtttacgtg gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa
     5701 tataccaaag tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg
     5761 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg
     5821 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc
     5881 agcctgccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg
     5941 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc
     6001 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa
     6061 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc
     6121 ggccgcgaag gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat
     6181 tcaggcaaaa ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct
     6241 atcaggaaat aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata
     6301 gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat
     6361 ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt
     6421 cagatatccg tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc
     6481 atccaatgct tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg
     6541 cctatcaaca aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg
     6601 cgctggttga gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc
     6661 ttaacggccc aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta
     6721 ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg
     6781 gtaaagaact ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta
     6841 tcgcgctgaa tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg
     6901 agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg
     6961 gcggcacgtt atttctcgac gaaattggag atatgccgct ggatgtgcag acgcgtttgc
     7021 tgcgcgtgct ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg
     7081 atgtgcggat tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt
     7141 tccgtgagga tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg
     7201 aacgtcggga agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac
     7261 tgggcgtaga agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt
     7321 ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg
     7381 ggcaggaagt gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga
     7441 gtacttcgca aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag
     7501 cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt
     7561 tactgacgac cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg
     7621 gctggggccg caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg
     7681 cttgtgtgta aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc
     7741 gataagttca gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa
     7801 ttaatctggt atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg
     7861 tgctgtgtgc cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg
     7921 agaaagtcgt aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc
     7981 ctgatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt
     8041 gcccagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
     8101 gtaaacgaag cgccattcac tctttaaatc acccgtgaga actgctgcat ccgcgctttc
     8161 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
     8221 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
     8281 agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg
     8341 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
     8401 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatccac ttgctgatag
     8461 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
     8521 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc
     8581 agcacgcctt cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata
     8641 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
     8701 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
     8761 ggcagatgcg cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc
     8821 agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
     8881 gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc
     8941 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg
     9001 cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
     9061 acttcgatcg aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc
     9121 agacggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg
     9181 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
     9241 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
     9301 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
     9361 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
     9421 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
     9481 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
     9541 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
     9601 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
     9661 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
     9721 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
     9781 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt
     9841 cgcctgcttc cagacgttcc agcagcgcat ccagacgctc atccgtttcc agtaattcca
     9901 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
     9961 tttcagtcac tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt
    10021 tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac
    10081 cacgacgttt tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt
    10141 ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg
    10201 ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc
    10261 aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg
    10321 gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg
    10381 tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg
    10441 tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg
    10501 ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt
    10561 gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg
    10621 catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt
    10681 atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata
    10741 atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag
    10801 acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa
    10861 gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac
    10921 cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt
    10981 gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg
    11041 gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc
    11101 ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag
    11161 atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac
    11221 aaactggcaa gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg
    11281 tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag
    11341 ctgcggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac
    11401 ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt
    11461 caatagatta caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct
    11521 tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca
    11581 gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc
    11641 tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt aatttttttt
    11701 cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac
    11761 ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact
    11821 ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat
    11881 ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac
    11941 ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat
    12001 cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc
    12061 agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc
    12121 gtccagcgtt tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc
    12181 cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg
    12241 aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat
    12301 cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc
    12361 tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc
    12421 acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac
    12481 ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg
    12541 aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg
    12601 ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta
    12661 agtcgacttc agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc
    12721 cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt
    12781 cagcggttta ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag
    12841 gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt
    12901 gagctcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat
    12961 gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt
    13021 gtgtttttgc agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc
    13081 ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc
    13141 tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct
    13201 gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa
    13261 cgcaatccca cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt
    13321 ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg
    13381 ctcgtgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc
    13441 tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct
    13501 gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt
    13561 cagtgtttct tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac
    13621 ttctggcgct tcgtctgcaa cactggtttc ctgcggcttc gcggctggtt ttgccccttt
    13681 ggcctgtaac catttgcccg cttcgacatc agcagtccag cgtttgaact catacttttt
    13741 gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag
    13801 ctccagttca acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt
    13861 gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt
    13921 ttctggctcg gtatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt
    13981 tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag
    14041 gaagtcgatg atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag
    14101 gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc
    14161 tttatcgcca gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt
    14221 accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt
    14281 aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg
    14341 tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac
    14401 cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata
    14461 catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg
    14521 ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat
    14581 gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag
    14641 tttttcaccg cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca
    14701 tgtggataac tttgtgcata aattatttac atcgctttta atttattatc atgcttttta
    14761 cagcatgatg tttgtcttta tttttcatgc agttaaaaat aaatccatcc ctaataattg
    14821 agataatggg attttttcta atgtggatag atatgaatta tttttctcct taaggatcat
    14881 ccgttatttg ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct
    14941 atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg
    15001 ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc
    15061 gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt
    15121 gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac
    15181 ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt
    15241 tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt
    15301 ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg
    15361 tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat
    15421 ccggagcgac gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg
    15481 catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag
    15541 caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg
    15601 ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac
    15661 aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat
    15721 gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt
    15781 aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg gcttctgacg
    15841 tctctcatat catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag
    15901 gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca
    15961 tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat
    16021 tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt
    16081 tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg
    16141 cggtttatgt gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt
    16201 tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta
    16261 gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta
    16321 ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt
    16381 ttgctaactc agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa
    16441 attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga
    16501 gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat
    16561 ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt
    16621 cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa
    16681 atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt
    16741 tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca
    16801 atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaac tttccatact
    16861 ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg
    16921 cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca
    16981 ctacgttcac ttttgccgct gccaatgctg ggatgacatt accatgcatt aacattgtga
    17041 tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat
    17101 tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aatacgggaa ttaaaaaagc
    17161 ctttgtcaaa gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg
    17221 catcgttaaa atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc
    17281 atgtgccacc accccagatt acggctaacg caacaataac acctgtagct gacttacgta
    17341 tcatataaat attccctgat aaaacagcaa aactttgaat atccacttat gctgaaataa
    17401 tgtccagcgg caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc
    17461 ccgtgaatat tcacgggctt tatgtaattt acattgaatt attttttctc ggacagatat
    17521 ttcaccgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
    17581 ggattcagct gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca
    17641 gctgcggctt tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg
    17701 tcgtactctt cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa
    17761 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
    17821 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
    17881 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
    17941 gaaatatgca gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc
    18001 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
    18061 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
    18121 ttcatgatct actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag
    18181 aacttttgcc tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa
    18241 ccacgggaaa tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata
    18301 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
    18361 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc
    18421 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
    18481 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg
    18541 cagaacggtg aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc
    18601 tttcagaaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
    18661 aggctcaatg agatattcat tcagaccgat ggtctggcga tgaataaaaa gctgtttgcg
    18721 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
    18781 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
    18841 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag
    18901 ctgcaacgca aattgatgtt cttcgagaat ttgatcggct gtccaacgtt cagggcgata
    18961 aaatttgacg acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata
    19021 gctgttaagc ggggtaagac cggaatccac ccggatccct tgctcaaaca gagcgtccat
    19081 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
    19141 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
    19201 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
    19261 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
    19321 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
    19381 gtttctgcaa aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga
    19441 gttcaataac ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag
    19501 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc tgatgacgac
    19561 gataacaggc gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg
    19621 attgctggac ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca
    19681 gctctctcac gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct
    19741 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
    19801 aatgcagcag gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc
    19861 ccatgattta gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
    19921 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cgcgctattg agcctttatt
    19981 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
    20041 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
    20101 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
    20161 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
    20221 ccggggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
    20281 tatgagctgc gcaaggctgg cgcggcacaa accatcgttg ccagccagca acgatgggcc
    20341 ttgatgacgg aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg
    20401 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
    20461 attgtgctgt ttcgcgatgc agccggacat cgacctgaag aattagtgat agacaggcat
    20521 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
    20581 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
    20641 agagtaggct ggatcagacg cagccagtgt cgcatccgac ctttttctgc accagcgcaa
    20701 aaaggcccga ctgggcct
//
LOCUS       DAJTNM010000067        20681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTNM010000067 DAJTNM010000000
VERSION     DAJTNM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.067"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(66..4526)
                     /gene="mukB"
                     /locus_tag="OO172_002489"
     CDS             complement(66..4526)
                     /gene="mukB"
                     /locus_tag="OO172_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5512637.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4526..5230)
                     /gene="mukE"
                     /locus_tag="OO172_002490"
     CDS             complement(4526..5230)
                     /gene="mukE"
                     /locus_tag="OO172_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5512638.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5211..6533)
                     /gene="mukF"
                     /locus_tag="OO172_002491"
     CDS             complement(5211..6533)
                     /gene="mukF"
                     /locus_tag="OO172_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5512639.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6530..7315)
                     /gene="cmoM"
                     /locus_tag="OO172_002492"
     CDS             complement(6530..7315)
                     /gene="cmoM"
                     /locus_tag="OO172_002492"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5512640.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7451..8230
                     /gene="elyC"
                     /locus_tag="OO172_002493"
     CDS             7451..8230
                     /gene="elyC"
                     /locus_tag="OO172_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5512641.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8207..9100)
                     /locus_tag="OO172_002494"
     CDS             complement(8207..9100)
                     /locus_tag="OO172_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5512642.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9254..10000)
                     /gene="kdsB"
                     /locus_tag="OO172_002495"
     CDS             complement(9254..10000)
                     /gene="kdsB"
                     /locus_tag="OO172_002495"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5512643.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9997..10179)
                     /gene="ycaR"
                     /locus_tag="OO172_002496"
     CDS             complement(9997..10179)
                     /gene="ycaR"
                     /locus_tag="OO172_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5512644.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10231..11463)
                     /locus_tag="OO172_002497"
     CDS             complement(10231..11463)
                     /locus_tag="OO172_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5512645.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11500..12486)
                     /gene="lpxK"
                     /locus_tag="OO172_002498"
     CDS             complement(11500..12486)
                     /gene="lpxK"
                     /locus_tag="OO172_002498"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5512646.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12483..14231)
                     /gene="msbA"
                     /locus_tag="OO172_002499"
     CDS             complement(12483..14231)
                     /gene="msbA"
                     /locus_tag="OO172_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5512647.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14268..16487)
                     /locus_tag="OO172_002500"
                     /pseudo
     CDS             complement(14268..16487)
                     /locus_tag="OO172_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16694..16978)
                     /gene="ihfB"
                     /locus_tag="OO172_002501"
     CDS             complement(16694..16978)
                     /gene="ihfB"
                     /locus_tag="OO172_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5512648.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17138..18811)
                     /gene="rpsA"
                     /locus_tag="OO172_002502"
     CDS             complement(17138..18811)
                     /gene="rpsA"
                     /locus_tag="OO172_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5512649.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18922..19605)
                     /gene="cmk"
                     /locus_tag="OO172_002503"
     CDS             complement(18922..19605)
                     /gene="cmk"
                     /locus_tag="OO172_002503"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5512650.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19778..20542)
                     /gene="ycaL"
                     /locus_tag="OO172_002504"
     CDS             complement(19778..20542)
                     /gene="ycaL"
                     /locus_tag="OO172_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5512651.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4969 a         5717 c         5023 g         4972 t
ORIGIN      
        1 cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt gctgccgcct
       61 taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc agggagttgc
      121 ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa gactttacgt
      181 accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc gatgatgagc
      241 tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc atccagtcgc
      301 gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg caggcggcga
      361 gattcatctt cccagctttg taccaccatc accagaatcg acataccggt accaatcgcc
      421 tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc acggttaacc
      481 tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat ggtctgcggc
      541 gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc caacgcttcc
      601 gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg ttcagagagc
      661 acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac gctgttcacc
      721 tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt ctgctcgcgc
      781 tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag tttctgttca
      841 cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc gatagcttcc
      901 accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg ctgataaacc
      961 gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat gcgcagcacg
     1021 tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc cttatccgac
     1081 atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa gcggcgctca
     1141 acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc ggtcactacc
     1201 tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt caggttgtcc
     1261 atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga acggttattg
     1321 ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc cccgctatca
     1381 gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc ttttttggtg
     1441 tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg cgctgcgtga
     1501 ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag acgttcacgc
     1561 agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga atagctaaag
     1621 tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc atcgcgctgc
     1681 atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg gtcgctctgc
     1741 aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg aacaaaacgc
     1801 gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc cgccaggctg
     1861 tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac gccttctttc
     1921 gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact caacgcccgc
     1981 tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc agactcaaac
     2041 gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg ctgggttttc
     2101 tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc ggcatggagg
     2161 ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg gaaacgtgaa
     2221 taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc atcaacgctg
     2281 aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc ttccgggcaa
     2341 tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac gatggcgtgg
     2401 cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac gtcgtcataa
     2461 atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg ctgatcttca
     2521 gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt gcgcgcgcct
     2581 acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg cagatattca
     2641 gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg gttgagactg
     2701 ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg agactgcagc
     2761 tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac gctatcggaa
     2821 agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc aatatcaaaa
     2881 ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg ctgctcacgc
     2941 agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc tgccaggtga
     3001 cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc gttacgcgcc
     3061 agcgggccgt tgattgccac caccagctga taagcctgct caaactggct gtgcgcggtt
     3121 tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc cagctctttc
     3181 gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg cagatggcac
     3241 agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc gcgcgtttgc
     3301 tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc cacttccagt
     3361 tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc tgccaccact
     3421 tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta gcgttcgatc
     3481 ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc cgcctgataa
     3541 tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc catatcgacg
     3601 tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg acgaaactcc
     3661 agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc cgccgccacg
     3721 tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg ggtgacacga
     3781 atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg gaacgcttta
     3841 cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat ggcactggag
     3901 atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc agatgcggag
     3961 cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc ggtaatggag
     4021 ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag ctcgttaagc
     4081 ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt cggctgcacc
     4141 gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc gcgtccggca
     4201 acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat ggtgtcgagc
     4261 atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg cgaaccgctg
     4321 gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg gatcagcgcc
     4381 gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga aagcgtcgtg
     4441 accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat cagcgtcagt
     4501 gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc tgactctctt
     4561 cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca tcacgaatca
     4621 ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg aagcggaaca
     4681 ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc atgcctaaac
     4741 gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg tcaacgtctg
     4801 aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc agggtgagca
     4861 gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt tccgggctga
     4921 gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag acggaacgcg
     4981 ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg cgaataagct
     5041 caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc aggaatgcat
     5101 gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg tccagcgccg
     5161 gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt tcaatatttg
     5221 tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca tttcgctggc
     5281 agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat aacaatacgc
     5341 gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac caccagacca
     5401 agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat gatcgccgcc
     5461 agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag ttccccagtc
     5521 acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg atcggcattg
     5581 gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg taaccgctga
     5641 gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg tacgtggcgg
     5701 tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg atcgagtttg
     5761 ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt catcgtcgca
     5821 tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag cgtatcctgc
     5881 aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga aatcgccgcc
     5941 cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg ctgttcgtcc
     6001 atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata tttcagtggc
     6061 gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc atctgctgcg
     6121 cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt agaaaactcg
     6181 cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg gtaaattgcg
     6241 ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac catgtcgttg
     6301 atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc atcactcaca
     6361 tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg ctcgccgttc
     6421 agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat ggagaagtca
     6481 ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact catactttat
     6541 ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta atatacggtt
     6601 cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg cgctgctggt
     6661 gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata atttgccaac
     6721 cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct ggcgaaagcg
     6781 tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg gcgaccatgt
     6841 tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg cctggacgca
     6901 acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg agcaccgcat
     6961 ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga gcggcgcaat
     7021 gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt gcgcggtcga
     7081 tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc attttgattg
     7141 cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg cccatttccg
     7201 ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct ttggtggtgc
     7261 cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc tgcattgctt
     7321 tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac agccacggcg
     7381 agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt taaaaaagga
     7441 accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc tgccacttcc
     7501 gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc gttttcaaaa
     7561 aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga gtttgcaacc
     7621 ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga ataattcgca
     7681 aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc agtgggcacc
     7741 gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc gtttatggcg
     7801 cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca atacggtgag
     7861 tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg agcaaattat
     7921 caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac aggcgattgg
     7981 tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga tgatcttttt
     8041 tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca tcgactcgcc
     8101 gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata gcgatcgtgt
     8161 tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag gcgagccacg
     8221 gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg gtattaacca
     8281 gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac gactccgcca
     8341 ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta tccattagtc
     8401 ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc atcgccagta
     8461 actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg tgaatcaaca
     8521 cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg cgaaacagga
     8581 tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg gtccatagca
     8641 cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg tcgaccgcgc
     8701 cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg atttggtctt
     8761 tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca ccacgcatcc
     8821 gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca ccataaatag
     8881 tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc agttgtcggg
     8941 cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc tggctgtcat
     9001 acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga ctgagttttt
     9061 cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc ctcataaaag
     9121 ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga tcaattgttg
     9181 aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt ggctgaagtg
     9241 tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca ggggtatcca
     9301 cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg taccacagaa
     9361 cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag ttgacgtaac
     9421 gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag ttatcgccaa
     9481 cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca cgtgagaagt
     9541 acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta aacgcttctt
     9601 ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga gcgaggttat
     9661 cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc acattaacga
     9721 tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc caccagacgt tctgttcctg
     9781 actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg gcgcgggcaa
     9841 catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc gcgcgttcaa
     9901 gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg ggcagacgcg
     9961 tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct catcagcagt
    10021 cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg ggaaagccag
    10081 gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt ttccgttgca
    10141 aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag atggatcggg
    10201 ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt cccagccagt
    10261 gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg ttgcctgctg
    10321 ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg tcgttggttt
    10381 aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc gatgcatttt
    10441 ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa aatagccata
    10501 ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa aaagctgctc
    10561 tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc tgtgcgttgc
    10621 agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat gcagccaaag
    10681 attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc tcgcttcgcg
    10741 ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact gttcgcggaa
    10801 tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg tttgcgaaac
    10861 gagatcgcgc tcatcatccc agtcaggcat gactcggtgg gttaaatcat aaacgcgctg
    10921 gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt ccagatgccg
    10981 tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct caaaatcggc
    11041 tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt ccgcctcatg
    11101 ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca gcatgcggtg
    11161 gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc agtattccat
    11221 taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc ccagacgact
    11281 gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt gcagcaagga
    11341 catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg gtttgtttaa
    11401 caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt gcggcagcga
    11461 cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag aagccagcga
    11521 ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt ctacaggcaa
    11581 ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt cagtcattac
    11641 cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag actgatgatc
    11701 ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg caaaaaagcg
    11761 cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa cgtcacaccg
    11821 cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga tttcaccgct
    11881 gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc ccgctcgctc
    11941 acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac catcaataac
    12001 gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg ttacgatgat
    12061 ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa cgggagaaac
    12121 cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg cctgtgctgt
    12181 ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat atccccgcga
    12241 tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga caacgaccgg
    12301 ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta cgggggcacg
    12361 ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca ccagaccata
    12421 caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc agattttttc
    12481 gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg ctcaagcaaa
    12541 tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat ttcgtctgcc
    12601 ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt ctgcaactca
    12661 tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt agcttcatcc
    12721 agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg ctgacgctga
    12781 ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt atccatctta
    12841 ttgatgaagt ccatggcgta ggccatacgc gccgcttctt caatttgctc acggctgtac
    12901 tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag atggacattc
    12961 tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg cagatcgtga
    13021 ccatccatca ggatttcacc ttcatcaata tcgtaaaaac gcgtgatcag gctggcaatg
    13081 gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc cggaattttc
    13141 aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt gacattgcgg
    13201 aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt ctcctgctca
    13261 ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa ctgggcgtta
    13321 acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa aacaacggta
    13381 atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata cagaacaaac
    13441 gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga ggctgaaacc
    13501 attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt cgtttccact
    13561 tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc tgcactggtg
    13621 gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg cttcgatact
    13681 acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga cagttgccaa
    13741 ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg cacaacagta
    13801 atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat acgtgacaac
    13861 agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat gtgaccaaac
    13921 aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca gtagctggag
    13981 acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg catccacacc
    14041 agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag gagcgataac
    14101 atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat cagacccgct
    14161 ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga gagatctttg
    14221 tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta cccgttatcc
    14281 actggcacgc caaaccactg ataataccaa cgaggtaaaa tttgctctct gaggctgcta
    14341 atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg agtatcaagc
    14401 cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa tcgccatgcg
    14461 ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat taatggcaac
    14521 gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg cacttgctgc
    14581 cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt tagaagaatg
    14641 ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt atcgttgcta
    14701 ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc ttcgccacgc
    14761 acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat cggctatata
    14821 tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag aataacgcct
    14881 tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc actatcccct
    14941 tcaggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc tatcaccatt
    15001 gccagccctt gcccgacatc aagcatgtac agctgccact cgtcaggtcg aggtttttgc
    15061 cacagcggcc agcacatcag caagcctcca gccacacaca ttactggcaa cgttcgccag
    15121 gcgtttaatc gccataccac cagtaagaac catggggaaa atgatagcca ttgccaacat
    15181 tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc cctgttccag gattaacggc
    15241 ccgctaaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag
    15301 ggaattgcaa atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt
    15361 tgcatgggca taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca
    15421 cgcaataccg gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt
    15481 gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc
    15541 agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt
    15601 aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt
    15661 cccgtcaacc aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag
    15721 tggatccagc gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac
    15781 gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc
    15841 atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata
    15901 accgcattcc acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc
    15961 aggctacaat tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc
    16021 tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca
    16081 cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg
    16141 ccatacagca caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga
    16201 ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg
    16261 ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa
    16321 aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag
    16381 gcgaagagag tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac
    16441 ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgcc cccctccctg
    16501 tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga
    16561 ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct
    16621 tccagttcct gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag
    16681 tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa
    16741 agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg
    16801 tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc
    16861 cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct
    16921 tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga
    16981 ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg
    17041 ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca
    17101 acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga
    17161 aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga
    17221 ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt
    17281 tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag
    17341 cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag
    17401 ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc
    17461 ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca
    17521 gggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt
    17581 ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa
    17641 ccaggccgtc gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga
    17701 ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag
    17761 ctttgcactg tttcagccct agggagatac gacgacgttc ttcgtcgata tccagaacca
    17821 taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc
    17881 aatccatttc ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc
    17941 cgtagtcagt caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag
    18001 cgatagctac ccacggatct tcgcccagct gtttcaagcc cagggataca cgggtacgtt
    18061 cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg
    18121 gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca
    18181 gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt
    18241 cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac
    18301 gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct
    18361 ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaaccaggca
    18421 ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa
    18481 caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac
    18541 gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag
    18601 caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct
    18661 cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga
    18721 tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact
    18781 cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc
    18841 cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg
    18901 gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc
    18961 tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc
    19021 cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc
    19081 caaaaggcgc tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg
    19141 cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac
    19201 cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg
    19261 acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac
    19321 ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc
    19381 attggtcgac acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga
    19441 cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga
    19501 gtccagcaga tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc
    19561 accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa
    19621 acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac
    19681 gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc
    19741 gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat
    19801 cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc
    19861 gaccgccatc caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc
    19921 cacgtttctt caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt
    19981 atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag
    20041 ccacgccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca
    20101 tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa
    20161 tttcgttgtc attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca
    20221 tcgcccatgc gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa
    20281 tattgttacc caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac
    20341 ttgcgacttg attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat
    20401 cggcatcgct taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg
    20461 tatcaatacc gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca
    20521 gcagtaattt agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg
    20581 tatcaacaac ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg
    20641 aaaaaaaacg ccgacaatgt gccgtaatgc cagtcagtta a
//
LOCUS       DAJTNM010000068        20597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTNM010000068 DAJTNM010000000
VERSION     DAJTNM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.068"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            135..287
                     /gene="hokA"
                     /locus_tag="OO172_002505"
     CDS             135..287
                     /gene="hokA"
                     /locus_tag="OO172_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5512652.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(474..686)
                     /gene="cspA"
                     /locus_tag="OO172_002506"
     CDS             complement(474..686)
                     /gene="cspA"
                     /locus_tag="OO172_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5512653.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(967..1257)
                     /locus_tag="OO172_002507"
     CDS             complement(967..1257)
                     /locus_tag="OO172_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR5512654.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1691..2401
                     /locus_tag="OO172_002508"
     CDS             1691..2401
                     /locus_tag="OO172_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5512655.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2451..3425)
                     /gene="ghrB"
                     /locus_tag="OO172_002509"
     CDS             complement(2451..3425)
                     /gene="ghrB"
                     /locus_tag="OO172_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5512656.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3529..4188)
                     /locus_tag="OO172_002510"
     CDS             complement(3529..4188)
                     /locus_tag="OO172_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5512657.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGVLSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4341..6674
                     /gene="bisC"
                     /locus_tag="OO172_002511"
     CDS             4341..6674
                     /gene="bisC"
                     /locus_tag="OO172_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5512658.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6643..7083)
                     /locus_tag="OO172_002512"
     CDS             complement(6643..7083)
                     /locus_tag="OO172_002512"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5512659.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7080..7643)
                     /gene="tag"
                     /locus_tag="OO172_002513"
     CDS             complement(7080..7643)
                     /gene="tag"
                     /locus_tag="OO172_002513"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5512660.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7801..8499
                     /locus_tag="OO172_002514"
     CDS             7801..8499
                     /locus_tag="OO172_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5512661.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8728..9936
                     /locus_tag="OO172_002515"
     CDS             8728..9936
                     /locus_tag="OO172_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5512662.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10260..11951
                     /gene="eptB"
                     /locus_tag="OO172_002516"
     CDS             10260..11951
                     /gene="eptB"
                     /locus_tag="OO172_002516"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5512663.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASAMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12043..12119
                     /locus_tag="OO172_002517"
     tRNA            12043..12119
                     /locus_tag="OO172_002517"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12077..12079,aa:Pro,seq:cgg)
     gene            12863..14470
                     /gene="dppA"
                     /locus_tag="OO172_002518"
     CDS             12863..14470
                     /gene="dppA"
                     /locus_tag="OO172_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5512664.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FESVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14778..15797
                     /gene="dppB"
                     /locus_tag="OO172_002519"
     CDS             14778..15797
                     /gene="dppB"
                     /locus_tag="OO172_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5512665.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15807..16709
                     /gene="dppC"
                     /locus_tag="OO172_002520"
     CDS             15807..16709
                     /gene="dppC"
                     /locus_tag="OO172_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5512666.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16720..17703
                     /gene="dppD"
                     /locus_tag="OO172_002521"
     CDS             16720..17703
                     /gene="dppD"
                     /locus_tag="OO172_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5512667.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17700..18704
                     /gene="dppF"
                     /locus_tag="OO172_002522"
     CDS             17700..18704
                     /gene="dppF"
                     /locus_tag="OO172_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5512668.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18734..20005)
                     /locus_tag="OO172_002523"
     CDS             complement(18734..20005)
                     /locus_tag="OO172_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5512669.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20481..20588
                     /locus_tag="OO172_002524"
     CDS             20481..20588
                     /locus_tag="OO172_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5512670.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4927 a         5097 c         5421 g         5152 t
ORIGIN      
        1 tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg gtcagtacaa
       61 agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt ggaaatcaac
      121 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      181 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      241 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcggcgg
      301 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac atttattctt
      361 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      421 aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      481 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      541 tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg tacgaacaca
      601 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      661 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      721 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      781 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      841 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      901 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      961 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
     1021 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatagcgac
     1081 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcgtaaccg
     1141 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1201 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1261 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1321 ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt cgattcagcc
     1381 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1441 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1501 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1561 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1621 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1681 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1741 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1801 tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1861 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1921 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1981 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     2041 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2101 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2161 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2221 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2281 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2341 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2401 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc ttagtccgcg
     2461 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2521 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2581 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2641 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2701 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2761 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2821 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2881 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcgcacgt
     2941 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     3001 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3061 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3121 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3181 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3241 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3301 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3361 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3421 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3481 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3541 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3601 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3661 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3721 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3781 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc tgctgctgtc
     3841 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3901 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3961 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     4021 cttcgaagaa gagagcacac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4081 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4141 cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg ttaatcctta
     4201 ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc gtgaacatga
     4261 agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc aatacatcta
     4321 acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt ctgactgccg
     4381 cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc tcgcgtggcg
     4441 cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag gttcacagca
     4501 atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg gaaaacccgc
     4561 aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg ctggatctta
     4621 ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt tttgctggtt
     4681 cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta caacgctata
     4741 tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt gcggcacagg
     4801 cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc agttggccgc
     4861 tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc aatacgctga
     4921 aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca ctgcgtgaca
     4981 gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat ttctttggcg
     5041 ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg ctggggatcg
     5101 cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat tgcaccacag
     5161 gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg aaaaacgccg
     5221 aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg gcggctattt
     5281 tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa cagtttggtg
     5341 agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc ggcacacccg
     5401 gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg cggcgttctg
     5461 cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg gataaaatcc
     5521 ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa cacaacggta
     5581 tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc aactttactc
     5641 atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg gtggtgatct
     5701 ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct gcgactacct
     5761 cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat ctggtgccga
     5821 tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt tttgccgagt
     5881 tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa agtcagctgc
     5941 aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag caggttgaat
     6001 tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg gaaaacccgg
     6061 acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg catccgttaa
     6121 aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac ggttacccgg
     6181 attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat gccgaaccag
     6241 aacagttgca ggtactttct gcccatccgg cgcaccgtct gcacagccag ctgaattaca
     6301 gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt catcctgacg
     6361 atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac tcccgtgggc
     6421 agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt tgcattcacg
     6481 aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac ggcgcagtga
     6541 acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg ggtaatacgg
     6601 cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg tttgaaccac
     6661 cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc aatcgacaat
     6721 gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat aaacctccag
     6781 catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa taccgcgtct
     6841 gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa tgctgacaaa
     6901 accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa gataggcatc
     6961 ccgcactagc ggaatgcagt cacgccagta attcgctttt ataaagggat gcccccaggt
     7021 tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg cttcccgaat
     7081 catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac cagcccacat
     7141 gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccaca ttttttcagt
     7201 gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa cgttgtggct
     7261 tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa cggttcgccg
     7321 ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat tttccctcga
     7381 tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg cattgctgcg
     7441 accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt tttgaggacg
     7501 gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa cagtttttta
     7561 ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag cgggccctga
     7621 ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt aaaaatttca
     7681 gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct actatgcccg
     7741 atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg agtcgtgtta
     7801 atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag cgtggtaatc
     7861 agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt tgatacgcaa
     7921 cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga ttacaacgat
     7981 attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt caatctggcg
     8041 gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt tcctttatat
     8101 gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac cacctcgatg
     8161 tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca tgccagcgtg
     8221 agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg ggcgcaaatc
     8281 agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct gagccggatg
     8341 acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga tatgttgctc
     8401 aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac taataatagc
     8461 gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca caattgaaac
     8521 cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa taaatttggt
     8581 gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc tgatggcatt
     8641 tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc agagattttt
     8701 cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg tacccgctgg
     8761 ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta tacctggagc
     8821 ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt cgctttctct
     8881 ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa attgcaggaa
     8941 cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt aggcttcttc
     9001 ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt gctggtcggt
     9061 ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg gttcccggag
     9121 cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag cctgggtttc
     9181 aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt tgtgatttgg
     9241 ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga cgcaccaaaa
     9301 caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc agagtcgatg
     9361 cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat gagcggcctg
     9421 tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga tgtggtttcc
     9481 gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct ggtgctgggc
     9541 attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt gatttcgctg
     9601 gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt tgcagcgatt
     9661 gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact ggtcagtgag
     9721 ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg tttcggtatc
     9781 ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt gaccttctac
     9841 gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg tcagccagag
     9901 cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag ctactgggct
     9961 tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact cgtaaaattt
    10021 gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct tttcaaactt
    10081 gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc gctttgctgt
    10141 ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac ttttcccctg
    10201 ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg tttgtttgca
    10261 tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca atctatattg
    10321 gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg cacgatttta
    10381 ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg gtgaccttct
    10441 ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg gcgtcgctgg
    10501 tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg gtcattggtt
    10561 atggcatcat cgcttccgcc atgaccaccg atatcgacct gtcaaaagaa gttgttggtc
    10621 tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt atctggaata
    10681 accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt attcgcagcc
    10741 tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg ctggatatcc
    10801 agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc ggtgtcgtag
    10861 cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc tgggcgcggg
    10921 tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt acataccagg
    10981 ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc acgcgttggg
    11041 accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc caggagaaaa
    11101 atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca ctgcgttgca
    11161 tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa gaacagaaca
    11221 ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg cagagcgaaa
    11281 tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag attggtgcgg
    11341 agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa atgcagcaat
    11401 cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa ggttcgcatt
    11461 ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa tgtattggtg
    11521 ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg gtgacctatg
    11581 tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg attgtgttct
    11641 acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc acgccgcgtg
    11701 aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca gataaatatc
    11761 tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc gacatgaaag
    11821 tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc tatacttcac
    11881 cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca aaagtggcag
    11941 cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg ctattaaggg
    12001 attgacgagg gcgtatctgc gcagtaagat gcgccccgca ttcggtgatt ggcgcagcct
    12061 ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat caccgaccaa
    12121 attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg aggatgagaa
    12181 cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc agcgtcgcat
    12241 caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc gaccaacaca
    12301 ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac ggatttgtga
    12361 cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact tatggctggc
    12421 gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag atatccacct
    12481 tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt gctaaatatt
    12541 aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa atagctgttt
    12601 gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc ggttggctgt
    12661 attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt tatggaggat
    12721 ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg ttgacccgac
    12781 cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt aaaacaacaa
    12841 acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga tgctgaagct
    12901 tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa ctctggttta
    12961 ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg gcaccaccta
    13021 tgacgcctct tccgtcccgc tttataaccg cctggttgaa tttaaaatcg gcaccaccga
    13081 agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa cctatacctt
    13141 ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga cgcgcgaaat
    13201 gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa acccgtacca
    13261 taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg agctgatcag
    13321 cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac cggaagcgcc
    13381 gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat atgctgacgc
    13441 gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa ccggtccgtt
    13501 ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg atggctactg
    13561 gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg cttccgtgcg
    13621 ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc cggcagacat
    13681 cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc tgaacgtcgg
    13741 ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc gccaggcact
    13801 gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg cgggtgtatc
    13861 agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc aggattacac
    13921 ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggcctggaaa aaggtttctc
    13981 catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc gccgcatggc
    14041 ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg tcacctacga
    14101 atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa tgatgggctg
    14161 gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct gcgccgcctc
    14221 tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc tgattcaacc
    14281 ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag cgcaggtggt
    14341 gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg aatcggtacg
    14401 caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg aaaacgtctc
    14461 tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc tgatgtgctc
    14521 cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt tgtaggccgg
    14581 ataaggcgtt aacgccgcat ccggcataaa caaagcgcac tttgtcaaca atctgtatac
    14641 ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca ttctctgtcc
    14701 gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc tgcgggtcac
    14761 tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc gtcatcccca
    14821 cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg ggcgatccgg
    14881 tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag ctgctggctg
    14941 aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg ggcgtaatgc
    15001 acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag ttcgtgccgc
    15061 gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg gcagtcggta
    15121 ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac actgcagttg
    15181 gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg ctgatcatgc
    15241 tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat atggtgttcc
    15301 tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc tggggtgaag
    15361 acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg ctgggtacta
    15421 ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg ctgggcgagg
    15481 attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt atcgtccatg
    15541 cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg ggaacattgc
    15601 tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg cgctggttga
    15661 ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg ctggtggcga
    15721 cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg aacccgcgta
    15781 ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa gtgattagcg
    15841 caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc aacaaaggcg
    15901 cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt gccaactgga
    15961 ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg ccagcctggc
    16021 aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt gatgtgctgt
    16081 cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg gtggtgttgt
    16141 cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc ctggttgata
    16201 acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg ttggcactgg
    16261 tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg accttcgttg
    16321 ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg aatcgcgatt
    16381 acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt attaacatct
    16441 tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct aacgccattc
    16501 tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg cctgagtggg
    16561 gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc gtgaccttcc
    16621 ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac ggtctgcgtg
    16681 acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt aaatgtagat
    16741 aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga ccgcatcagc
    16801 tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc cggtaagtcg
    16861 gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat ggcagaaaaa
    16921 ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg caacctggtg
    16981 ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc atgctacacc
    17041 gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa aagtacccgc
    17101 cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc ttcgcgtctg
    17161 gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat cgccatggcg
    17221 attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct ggacgtgacc
    17281 attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa catggcgctg
    17341 gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat cattgtgatg
    17401 tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc gccgcgtcac
    17461 ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa agaacgtctg
    17521 gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg cctgcttaac
    17581 ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct gaatatgctc
    17641 gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag gcccacacta
    17701 tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac ctgaaaaaac
    17761 attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg ctggacggcg
    17821 tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct ggctgcggta
    17881 aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc gagctgtatt
    17941 atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt cggcagaaaa
    18001 tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa gttgggcaaa
    18061 ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt cgggaaaaag
    18121 ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc tatccgcaca
    18181 tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg ctcgacccgg
    18241 atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt gcgcaggtgc
    18301 tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt atctcccacg
    18361 acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta ggccgctgcg
    18421 tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac acccaggcgc
    18481 tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc aaactcaccg
    18541 gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc cgctgtcgtc
    18601 ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga ggtcaactgg
    18661 tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca aaaccggggc
    18721 catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc aaaatgccgt
    18781 aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg actttataga
    18841 tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc aactgaaaga
    18901 acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg ataaaagcgc
    18961 agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt cggcttaaca
    19021 gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca aagaatgcgg
    19081 tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt ccagggatca
    19141 cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg gcttcttcgt
    19201 ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg agcgtgatat
    19261 aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct gcttcccgtt
    19321 tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa aagaagcaaa
    19381 atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag gcggttatat
    19441 tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc cctactttga
    19501 ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac gcaatcgcta
    19561 tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg gcatcggtta
    19621 aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag tagataaaaa
    19681 tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag ttcttcccca
    19741 ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa agggttttta
    19801 aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg gtaataaata
    19861 cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca ccaatcgcca
    19921 tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat ttctgattgt
    19981 gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg cctcgcaaaa
    20041 atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc agtatgtaaa
    20101 aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac aaaattgcgc
    20161 atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca aataaagaaa
    20221 ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt aaaaagcaat
    20281 cgtaaaatca gcggcaagcc ggaagggttc cggtgaggcg caatgttgcg ggggctttat
    20341 ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt atgtacctga
    20401 caacataacg ggggctaatc ttgaacctag accactcaag aatagccgcg aaacgttgtc
    20461 attacaacac aggcggctat atgacgctcg cagagctggg catggccttc tggcatgatt
    20521 tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg ctgaacaagc
    20581 ggaagtagcg tgtcatg
//
LOCUS       DAJTNM010000069        20548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTNM010000069 DAJTNM010000000
VERSION     DAJTNM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.069"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO172_002525"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO172_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5512671.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO172_002526"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO172_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5512672.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO172_002527"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO172_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5512673.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO172_002528"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO172_002528"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5512674.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFCKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO172_002529"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO172_002529"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5512675.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4448..4813
                     /locus_tag="OO172_002530"
     CDS             4448..4813
                     /locus_tag="OO172_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5512676.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4963..5481
                     /locus_tag="OO172_002531"
     CDS             4963..5481
                     /locus_tag="OO172_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5512677.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5471..6696
                     /gene="dgcN"
                     /locus_tag="OO172_002532"
                     /pseudo
     CDS             5471..6696
                     /gene="dgcN"
                     /locus_tag="OO172_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6712..7194
                     /locus_tag="OO172_002533"
     CDS             6712..7194
                     /locus_tag="OO172_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5512678.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQLQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="OO172_002534"
     CDS             complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="OO172_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5512679.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="OO172_002535"
     CDS             complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="OO172_002535"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5512680.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="OO172_002536"
     CDS             complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="OO172_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5512681.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="OO172_002537"
     CDS             complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="OO172_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5512682.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="OO172_002538"
     CDS             complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="OO172_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5512683.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10938..11729
                     /locus_tag="OO172_002539"
     CDS             10938..11729
                     /locus_tag="OO172_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5512684.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11750..13036
                     /locus_tag="OO172_002540"
     CDS             11750..13036
                     /locus_tag="OO172_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5512685.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="OO172_002541"
     CDS             complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="OO172_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5512686.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13807..14685
                     /gene="nadK"
                     /locus_tag="OO172_002542"
     CDS             13807..14685
                     /gene="nadK"
                     /locus_tag="OO172_002542"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5512687.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14771..16432
                     /gene="recN"
                     /locus_tag="OO172_002543"
     CDS             14771..16432
                     /gene="recN"
                     /locus_tag="OO172_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5512688.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16581..16922
                     /gene="bamE"
                     /locus_tag="OO172_002544"
     CDS             16581..16922
                     /gene="bamE"
                     /locus_tag="OO172_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5512689.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16984..17274)
                     /locus_tag="OO172_002545"
     CDS             complement(16984..17274)
                     /locus_tag="OO172_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5512690.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17264..17701)
                     /gene="ratA"
                     /locus_tag="OO172_002546"
     CDS             complement(17264..17701)
                     /gene="ratA"
                     /locus_tag="OO172_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5512691.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17872..18354
                     /gene="smpB"
                     /locus_tag="OO172_002547"
     CDS             17872..18354
                     /gene="smpB"
                     /locus_tag="OO172_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5512692.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18569..18931
                     /gene="ssrA"
                     /locus_tag="OO172_002548"
     tmRNA           18569..18931
                     /gene="ssrA"
                     /locus_tag="OO172_002548"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19136..20377
                     /locus_tag="OO172_002549"
     CDS             19136..20377
                     /locus_tag="OO172_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5512693.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDDDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5305 a         5368 c         4822 g         5053 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac catcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcag aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccaa gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat atataaattt acaccccttt gaaaacaatc cgctatgctt tgaaaaagga
     4441 gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct ttactggtta gcggatgtca
     4501 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4561 ggaagatgcg atatcggaca gcgccataaa agatgacgat gcatttagcg attcacaggc
     4621 ggatcgcggt ctatacctta aaggatatgc cgaagggcaa aagaaaactt gccagaccga
     4681 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgca acaatgttga
     4741 aaatgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
     4801 acgattaaat taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt
     4861 ttatttctca cattgatgac ggtcgcatga aaaaatgata ttttaatgag catcctgaaa
     4921 aaatatcgat gcatttcgag cgaagatggt ggggatccct gaatgcgttt ttctcaccga
     4981 ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca agagccttct
     5041 gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct
     5101 gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta
     5161 caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag
     5221 gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg
     5281 gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca aaataccgag
     5341 tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta
     5401 aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca
     5461 cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc
     5521 attacgcaac atcagtatga ccagcatatt tatcactatg atgttgatct ggttgctgct
     5581 ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc
     5641 agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac
     5701 tgaaacactg gcagcgctgg gccagcaagg gcattttcaa ctgcagaagt acgtgataag
     5761 cagcaaaata ttctggcatc ctggcattac ccccgtaagg atccaggcga tactttcagc
     5821 aatttcataa gccactggct cttccctgcc cccatcattc agccgattcg tcacaatggt
     5881 gaaaccattg gcgaagtacg cttaaccgct cgcgacagtt caattagcca ttttatctgg
     5941 ttttcactcg ccgtactgac cggttgtatt ctgctggcat caggcatcgc aattaccctc
     6001 acccgccatt tgcacaatgg cctggtggaa gcactgaaaa atatcaccga tgtcgtacat
     6061 gatgtgcgtt ccaaccgcaa tttttcccga cgagtttcgg aagaacgtat cgctgagttt
     6121 caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg gcagcttcgt
     6181 ttacaggcca aaaatgcgca gcttctgcgt accgcgctac atgacccatt aaccgggctg
     6241 gctaaccgcg cagcgtttcg tagcggcatc aatacgttga tgaacaattc cgatgcccga
     6301 aaaacgtcgg cgttactatt tcttgatggc gataatttca aatatatcaa tgatacctgg
     6361 ggtcatgcga cgggcgatag ggtcttgatt gaaatcgcaa aacggttagc tgaatttggc
     6421 gggctgcgac ataaagcata ccgcctgggc ggcgatgaat tcgctatggt gctctatgat
     6481 gtacagtcgg aatctgaagt gcagcagata tgctcagcac tgacacaaat ctttaatctc
     6541 ccgtttgatc ttcataatgg ccatcagacc accatgacat taagcattgg ttacgcgatg
     6601 accattgagc acgcctctgc ggaaaaatta caagagcttg ccgatcacaa tatgtatcag
     6661 gccaaacacc agcgtgccga aaagctggtg agataacaag gatatatcga tatgataaag
     6721 cacctggtag cacccctgat tttcacctca ctaatactga ctggctgcca gtcccctcag
     6781 ggaaagttta ctcctgagca agtcgccgct atgcaatctt atggatttac tgaatccgcc
     6841 ggcgactggt cgctgggctt atcagatgcc attctgttcg caaaaaatga ctacaaattg
     6901 ctcccggaaa gccagcaaca gctccaaacc atggcagcca aattggcctc gacagggcta
     6961 acacatgccc gtatggatgg acacaccgat aactatggtg aagacagtta caacgaaggc
     7021 ttatcattga aacgggcgaa tgtcgtggcc gatgcatggg ctattggtgg acaaattcca
     7081 cgcagcaatc tcaccacaca gggtttagga aaaaaatatc ccatagccag taacaagacc
     7141 gcccagggcc gcgccgagaa ccgccgcgtc gcagtggtga ttacaacccc ttaaaacacg
     7201 agcaaacaga caaaaaaagg gccagccaat tgcccagccc ttcttaacag gatgtcgctt
     7261 aagcgaaatc ttagttaaga cgctctttga tacgagcagc cttaccagta cgctcacgca
     7321 ggtagtacag tttagcttta cgaacagcac cacgacgttt gacagaaatg ctgtcaacta
     7381 ccggagagtg agtctggaag acacgctcaa cgccttcgcc gttggaaatt ttacgaacag
     7441 tgaatgcaga gtgcagaccg cggttacgaa tagcgataac cacgccctcg aatgcctgca
     7501 gacgtttttt ggaaccttca acaacccata ctttcacttc cacggtatca cccggacgga
     7561 aggaaggtac gtcctgcttc atctgctctt gttcaagttg cttaataatg ttgctcataa
     7621 tttaatctct tatcctgggt aaactgatct ctcgggggct tacgccatcc catcatgttt
     7681 atgttgctgt tgtgcgtgtt ccgttttgaa ctccgccagc aaccttgctt gctcttcagt
     7741 cagagccagg ttttccagaa gttcaggtct tctaagccag gtacggccca gcgactgttt
     7801 caaacgccag cgacgtatct cggcatggtt gcccgacagt aacactggcg gaacttccat
     7861 cccttctaac acctcaggcc gcgtatagtg cgggcaatcc agcaatcctt cagcaaagga
     7921 atcttccgtt gccgaggctt catgtcccag tactcccgga ataaaccggg aaacggagtc
     7981 aatcagcgtc attgctggta actcaccacc actgagaacg taatcgccga ttgaccattc
     8041 ttcgtcaatt tcggtttgga tcacgcgctc atctatacct tcgtagcgac cgcacaccag
     8101 aatcaatttt tgattcgttg ccagttcgct gacgcccgct tgatcaagct tgcgtccctg
     8161 tggtgacaga taaatcacct ttgcgccttc acccgccgcg gcttttgctg catgaatggc
     8221 gtcccgcaag ggttgcacca tcattaacat ccccggtccg ccgccgtaag gacgatcgtc
     8281 cacggtacgg tgccggtcat gcgtgaagtc gcgaggactc caactctgga tgctcagcag
     8341 gccattttta actgcccggc cagttacccc gtaatcggta attgcgcgga acatttcagg
     8401 aaacaggcta attatgccaa tccacatagc gccgtctttt accgtttatc cggtggttta
     8461 aaaaccagga tcccaatcta cttcgattga acgagtagtg agatcgactt tcttgataac
     8521 ctgcccatcg aggaacggta cgagacgttc cttgatacca aacgcatctt tcaggtttgc
     8581 cttaatgacg agaacgtcat tagatccggt ttccatcata tcgacgactt taccgagatc
     8641 gtagccttca gtggttacta cctggcagcc catcaggtct ttccagtagt agtccccctc
     8701 ttcaagctga ggcagctgcg atgaatccac gacaatttca caattcgtca gcaggttcgc
     8761 cgcatcacga tcgtcaacgc ctttcagctt gatgatcatg tcctgattgt ggtgcttcca
     8821 gctttccagc tggacttgct gccactgacc cgccttctgg ataaaccagg gctgatagtc
     8881 aaaaatgctt tcggcgtctt cggtggaaga aaacactctg agccacccac gaataccgta
     8941 agacgaaccc atttttccca aaacgatggg atcaacaggt gcttgcgcgg tgagttgttt
     9001 gctcatcatg accaccgtga cagattaagc tgctttgttt acttctttga tcagcgcagc
     9061 aacgcgatca gaaatagttg cgccctggcc aacccagtga gcgatgcgat ccagatccag
     9121 gcgagtgcct tcttcttttt cgctagcgat tgggttgaag aaaccaacgc gctcgatgaa
     9181 gcgaccgttg cgtgcattac ggctgtcagc gacaacaacc tggtagaacg gacgcttttt
     9241 agcgccgtga cgtgctaaac gaatagttac cataacatcc tcttgtgtga ataaaacaac
     9301 cggaccccat cgaggaacgg agtccggtgt catattaaaa gcccgaaaat tttactgatt
     9361 tctggagaaa atgcaatcag cagttaataa ctctgtcgca taaggtcatt tagcgaccag
     9421 gaaatcccgg cggcatcatt cccttcatgc ttctcatcat cttcgccatt ccgcctttct
     9481 tcattttctt catcatgcgc tgcatgtcgt cgaactgctt cagaaggcgg ttaacgtcct
     9541 gtacctgcat cccgcaaccg gcggcgatac gacgcttacg cgaacctttg atgatttctg
     9601 gcttagcgcg ctctttcatc gtcattgagt tgatgatggc ttccatacgc accagcactt
     9661 tatcgtccat ctgcgacttg acgttatccg ggatctgccc catgcccggc agtttgccca
     9721 tcaagctggc catgccgccc atatttttca tctggcgcag ttgctcaaga aagtcgttga
     9781 gatcgaagcc gtcgcctttt tttagcttgc tggctaattt ctctgcctgc gcgcggtcaa
     9841 ctttgctttc gatatcttcg atcagcgaca gtacgtcgcc catgccgaga atacgcgacg
     9901 cgatgcggtc cggatggaac ggctccagcg cctcagtttt ctcgccaaca ccgaggaact
     9961 tgatcggttt gccagtgatg tgacgaatag agagcgccgc accgccgcgg gcatcgccgt
    10021 ccactttggt caacactacg ccggtaagcg gtaacgcttc attgaatgct tttgccgtat
    10081 tggccgcatc ctgaccggtc atggcgtcaa ccacaaacag ggtttcaacc gggttaatcg
    10141 acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc aacgtgcaga cgaccagcgg
    10201 tatccaccag cagcacgtcg tagaatttca gtttggcttc tttcagcgcc gcgttaacaa
    10261 tatcaaccgg cttctgacca acatcagaag ggaagaaatc aacgcccacc tgctctgcca
    10321 gcgtctcaag ctgtttgatt gccgccgggc gatagacgtc agcagaaacc accagcactt
    10381 tcttcttgtg cttctcgcgc aggaaattac cgagtttacc aacgctggtt gttttaccgg
    10441 caccttgcag gcctgccatc agcacgaccg caggcggttg cgcagccaga ttcagggtct
    10501 ggttctcttc gcccatcgcc gcaaccagtt cgttacggac gattttgacg aactcctgcc
    10561 ccggcgtcag gctcttatta acttcatgac caaccgcttt ctcttttacg cgattgataa
    10621 actcacgcac taccggcaga gcgacgtccg cctccagcag cgccatgcgc acttcgcgca
    10681 gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc actgatattg cgcagcgtgc
    10741 gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg cctggggtgg aaacggttgg
    10801 ccgcaatcgc gacacatcat cagtattttg ccgcagtata acatgaaggc gtctttgttg
    10861 ttatgcaacg gttggagcag cgttcacctg acgctatact gcttctcttt cttattgctc
    10921 aaactgtcga cattactatg cccgtttttg ctctgctcgc gcttgtcgcc tactccgtca
    10981 gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg cggctggcgg cgcatggcta
    11041 ttatttctgc ggtcattgcg ctggtctgcc acgcaatcgc tctggaagcc cgcatcctgc
    11101 ccgacggtga tagcggacaa aacctcagcc tgctgaacgt tggttcattg gtcagtttgg
    11161 tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg tggctggttg ctgctaccca
    11221 ttgtctatgc ctttgcgctt atcaacctgg cgctggcgac cttcatgccc aatgaataca
    11281 tcacccatct ggaagcaacg cctgggatgc tggtgcacat tggcttatcg ctcttttcct
    11341 atgccacgct aattatcgcc gccctgtacg cgctgcaact ggcgtggatt gattaccaac
    11401 tgaagaacaa gaagctggca tttaaccagg aaatgccgcc attgatgagt atcgagcgta
    11461 aaatgttcca catcacgcag attggcgtgg tgctgctaac gctcacgctt tgcactggcc
    11521 tgttctacat gcacaaccta tttagcatgg aaaatatcga caaggctgtg ctctctatcg
    11581 tggcgtggtt tgtctatatt gtgctgctgt ggggacatta ccatgaagga tggcgtggac
    11641 gccgcgtcgt ctggtttaac gttgcgggcg cggtaattct gacactggcc tacttcggca
    11701 gccgaattgt ccagcagtta atcagctaaa cccagaaaag gagtttcccc tggaacacat
    11761 ttctactact acgttgatca ttattctgat catcatggtg gtcatttcag cctatttttc
    11821 cgggtccgaa accggaatga tgaccctcaa ccgctatcgt cttcgacata tggcgaaaca
    11881 gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt aagccagacc gcctgataag
    11941 cctggtgtta atcggcaata acctggtcaa tattcttgcc tccgcgctcg gcactattgt
    12001 cgggatgcgt ttgtacggcg atgcgggcgt ggcaattgcg actggtgtgc tgacttttgt
    12061 cgtactggtt tttgctgagg tattgccgaa aaccattgcc gcgctgtacc cggaaaaagt
    12121 cgcttatccg agtagttttc tgctggctcc gctgcaaatt ttgatgatgc cgctggtctg
    12181 gttgctgaat gctatcaccc gtatgctgat gcgcatgatg ggtatcaaaa ccgatatcgt
    12241 ggttagcggc tctttgagca aagaagagtt gcgcactatc gtgcatgaat cgcgctcaca
    12301 aatttcccgt cgcaatcagg atatgctgct gtcggtgctc gatctggaaa aaatgaccgt
    12361 tgatgacatc atggtgccgc gcagtgaaat tatcggtatt gatatcaacg atgactggaa
    12421 atctattctg cgccaactct cccactcacc tcacgggcgc atcgtgcttt accgtgattc
    12481 gctggacgac gccatcagta tgctgcgagt acgtgaagcc tggcggttga tgtcggagaa
    12541 aaaagagttc accaaagaaa ccatgctgcg cgccgcggac gagatctatt ttgtgcctga
    12601 aggtacgccg ctcagcacgc agttggtaaa gtttcagcgc aacaaaaaga aagtcggcct
    12661 ggtcgtcaac gagtatggag acattcaggg gctggtgacg gttgaagata ttctggaaga
    12721 gattgtcggc gatttcacta cgtcgatgtc gccaacactt gccgaagagg tcacgccgca
    12781 aaacgacggt tcggtgatta tcgatggcac cgccaacgtg cgggaaatca acaaagcctt
    12841 taactggcat ctaccggaag atgatgcccg cacggttaat ggcgtcattc ttgaggcact
    12901 ggaagagatc cctgtcgcag gcacccgcgt gcgtattggc gagtacgata tcgatattct
    12961 cgacgtacag gacaatatga ttaagcaggt aaaagttttt cctgtgaaac cgctgcgcga
    13021 gagtgtggcg gagtaacgaa aacggccctg cattcgcatg cagggccgtg aattattacg
    13081 aaaacagaaa ttacgctttt gctttcgcta cagttaccat cgccgcacga atcgtacgac
    13141 cattcagcgt ataacccttc tgcataatgc ccagtacgtt acctggcgca acgtcatcag
    13201 attccaccat tgcgatagcc tgatgcacat tcgggtccag cggcacgtta gtttcggcga
    13261 tcacttcaac accaaactta cgcacaacat ccagcatcga cttcagcgtc agctcaatgc
    13321 cttcaaccat cgcagacata tccgggttag ctttatcagc cacttccagc gcacgatcca
    13381 ggctatcaat caccggcagc aattcgttga tgaatttctc cagcgcgaat ttgtgggctt
    13441 tttcaatatc cagttcagta cgacgacgca ggttttccat ttcggctttt acgcgcaaaa
    13501 taccgtcacg ttcacgggtc tgggcttcag ccagctgagc ttcgagattc gcaatttttt
    13561 catcgcgcgg atccacctgc tcagcagaag cttctggctc aactgcctca atctcttcgt
    13621 gctgatccat gataatttct tccggggctt gcccctcagg cgttttctgt tctttactac
    13681 tcatgaattt ctccgcgttt ttttcgcatt catctcgcta acttcgctta ttatggggat
    13741 cagtttcagg gtttcaaggg aagcactcac attgtcatca atcttcgcaa caaggacctc
    13801 ggaaaaatga ataatcattt caagtgcatt ggcattgtgg gacacccacg gcaccctact
    13861 gcactgacaa cacatgaaat gctctaccgc tggctgtgca caaaaggtta cgaggtcatc
    13921 gttgagcaac aaatcgctca cgaactgcaa ctgaagaatg tgaaaactgg cacgctcgcg
    13981 gagattgggc aactggctga tctcgcggta gtcgttggtg gcgacggtaa tatgctgggc
    14041 gcggcgcgca cgctcgcccg ttacgatatt aaagttattg gaatcaaccg tggcaacctg
    14101 ggtttcctga ctgaccttga ccccgataac gcccagcaac aattagccga tgtgctggaa
    14161 ggtcactaca tcagcgagaa acgttttttg ctggaagcgc aagtctgtca gcaagattgc
    14221 caaaaacgca tcagcaccgc gattaacgaa gtggtacttc atcctggcaa agtggcgcat
    14281 atgatagagt tcgaagtgta tatcgacgag atctttgcgt tttctcagcg atctgatggg
    14341 ctaattattt cgacgccaac aggctccacc gcctattccc tctctgcggg cggtcctatt
    14401 ctgaccccct ctctggatgc gattaccctg gtgcccatgt tcccgcatac gttgtcagca
    14461 cgaccactgg tcataaacag cagcagcacg atccgtctgc gtttttcgca tcgccgtaac
    14521 gacctggaaa tcagttgcga cagccagata gcactgccga ttcaggaagg tgaagatgtc
    14581 ctgattcgtc gctgtgatta ccatctgaat ctgattcatc cgaaagatta cagttatttc
    14641 aatacattaa gcaccaagct cggctggtca aaaaaattat tctaatttta cgccagcctc
    14701 tttactgtat ataaaaccag tttatactgt acacaataac agtaatggtt tttcatacag
    14761 gaaaacgact atgttggcac aactgaccat cagcaacttt gctatcgttc gtgagcttga
    14821 gattgatttt catagcggca tgaccgtaat aactggcgag accggtgcgg gtaaatctat
    14881 tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct gaagccgaca tggtgcgtac
    14941 cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg aaagatacgc cagcggccct
    15001 gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa tgtttgcttc gtcgcgtgat
    15061 cagcagtgat ggtcgctccc gtggtttcat caacggtaca gctgttcctc tgtcacaact
    15121 gcgcgaactg ggtcagttgc tgattcagat ccatggtcag cacgctcatc aattactcac
    15181 caaacctgag caccaaaaat tcctgcttga tggctatgcc aatgaaacct ctctacttca
    15241 ggaaatgacc gcacgttatc agttgtggca tcaaagctgc cgtgacctcg cgcatcatca
    15301 acagttaagt caggaacgcg ccgcccgtgc ggaactgctg caataccaat taaaagaact
    15361 taacgaattt aatccgcagc ccggagagtt tgagcaaatc gacgaagagt acaaacgtct
    15421 ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca ttggcattaa tggccgacgg
    15481 tgaagacgca aacctgcaaa gtcagcttta cacggctaaa caactggtga gcgaattgat
    15541 tggcatggac agcaaactgt ccggcgtact tgatatgctg gaagaagcta ccatccagat
    15601 tgctgaagcc agcgacgaac tgcgccacta ctgcgatcgt ctggatctcg atcccaaccg
    15661 actgtttgaa cttgaacagc gcatctcaaa acagatttcg ctggcacgta aacatcacgt
    15721 cagccctgag gcattaccac agtattacca gtcgctactg gaagaacagc agcaactgga
    15781 cgatcaggcc gactcacaag aaacgctcgc gctggcggta acgaaacatc atcagcaggc
    15841 actggaaacc gcgcgcgcat tacaccaaca acgccagcaa tatgcagaag aacttgcaca
    15901 gctgatcacc gacagtatgc atgcgctctc aatgccgcat gggcagttta cgattgatgt
    15961 caaatatgac gagcatcacc tgggcgctga cggtgccgat cgtattgagt ttcgggtaac
    16021 caccaaccca ggtcagccaa tgcagcctat tgccaaagtc gcatccggtg gtgaattgtc
    16081 ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa atggaaaccc cggcactgat
    16141 ttttgatgaa gtggatgtag ggattagcgg tccaacagcg gcagttgtcg gcaaactgct
    16201 gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc cacctgccac aagtcgcggg
    16261 atgtggtcat caacactatt ttgtcagcaa agaaaccgat ggtgcgatga cagaaacaca
    16321 tatgcaatct ctggataaaa aagcgcggtt acaagagctg gcgcgcctgc ttggtggcag
    16381 tgaagtcaca cgtaacacac tggcgaatgc gaaagaactg cttgcagcgt aaactttttt
    16441 cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga ccggaaagca aaaggtttta
    16501 aagtgatgaa aggtctatta tcatcggcat attacagatg agccacgtac tgctcgggcc
    16561 cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac tgctgcagca gcagtactat
    16621 tgatgttgac cgcaggctgt tccactctgg agcgagtggt ttaccgtcct gacatcaacc
    16681 aggggaacta tctgaccgct aacgacgtat ccaaaatacg tgttggcatg acgcaacaac
    16741 aagttgcgta cgcattgggt acaccgctga tgtccgatcc atttggtacg aatacctggt
    16801 tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac gcagcaaacg ctgacgctga
    16861 cctttaacag tagcggtgtg ttgaccaata ttgataacaa acctgcgctg agtggtaact
    16921 aataataaag ttgctctcaa agacgttaaa aaaggtgctc aatgagcacc ttttttctgt
    16981 ctgttattta ttcgctgatt tttctgctcg ttgcctgcga agctctttcg gatcggcaat
    17041 gagagggcga taaatctcca cccgatcgcc atcatgcaca ctatcgctta gttttgccgg
    17101 acggctgtaa atgccgactt tgtttttagt taaatcgata tcggtacgca attccagcaa
    17161 gccactggcg cgaatagctt cttcaaccgt cgcgccctcc tgcagcgtca ctcgctgcag
    17221 gtactgcttc tcaggtagcg cataagccac ctcaacggca attttaccta gcactgtaaa
    17281 cctctttcgc acgaaccgta aaagcctgga ccatattagc cgccagctct ttaaacacgc
    17341 gaccaaaggc gagttcaatc aacttattgg taaactcaaa gtcgagatga aactcgatac
    17401 gacacgcctc ctggctcagc ggcgtaaact tccatccacc aatcaatttc ttgaacggcc
    17461 catccaccag attcataaga atactttggt tactggtcaa ctggttgcgg gtagtaaacg
    17521 ttttgctgat cccagcctta gagacatcta ccgccgcagt catctgccca ggagtggact
    17581 ccagaatccg acttccggta caacctggca aaaactgagg ataagactga acgtcattca
    17641 ctaactgata catttgctcc gcgctgtagg gtaccagtgc ggtccggcta atctgaggca
    17701 taacaatttc catcaacaaa aatccaacaa ataatatcat ttatcctgct gtaaaaaaaa
    17761 cgctatcccg gcgctgggta acatcgggtt catgctaaga tagagccttg tcccccgcag
    17821 gattgatatg gggtgttttc gatttcagat taccgatgat tcacgacgct tatgacgaag
    17881 aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta acaagcgcgc ccgtcacgaa
    17941 tactttatcg aagaagagtt cgaagcggga cttgccctgc aaggctggga agttaaatcc
    18001 ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc ttctgcgtga cggagaggca
    18061 tttctgtttg gcgctaacat cacgccaatg gccgtggcct ccacgcatgt ggtgtgcgat
    18121 cctacccgta cccgcaagtt acttctcaac cagcgcgaac tggactcatt gtacggtcgc
    18181 gtcaatcgag aaggctatac cgtagtggcg ctctccctgt actggaaaaa tgcctggtgc
    18241 aaagtgaaaa tcggcgtcgc caaaggtaaa aaacagcacg ataaacgttc agatatcaaa
    18301 gaacgcgaat ggcaggtgga taaagcacgc atcatgaaaa acgcccaccg ttaaacctgc
    18361 actccaatta ttgaccagtt cctcaccgcg cctccctctc cggcggcgcg aatgaacatc
    18421 ttattggcta tcacatccga cacaaatgtt gccatcccat tgcttaatcg aataaaaatc
    18481 aggctacatg ggtgctaaat ctttaacgat aacgccattg aggctggtca tggcgctcat
    18541 aaatctggta tacttacctt tacacattgg ggctgattct ggattcgacg ggatttgcga
    18601 aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa aatagtcgca
    18661 aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct ctccctagcc
    18721 tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg tggaagccct
    18781 gcctggggtt gaagcgttaa aacttaatca ggctagtttg ttagtggcgt gtccgtccgc
    18841 agctggcaag cgaatgtaaa gactgactaa gcatgtagta ccgaggatgt aggaatttcg
    18901 gacgcgggtt caactcccgc cagctccacc aaaattctcc atcggtgatt accagagtca
    18961 tccgatgaag tcctaagagc ccgcacggcg caagccctgc gggctttttt gtgccccgaa
    19021 tttgtcccgc gaagtctgat gccaactaat taaatccgat ccttttaggc accttgttag
    19081 gcacttcata aagctatatt gtctttgagg tgcctaaaac tatggaaacc cggcaatggc
    19141 aagacaaacc aaacctctat ccgttaagga aatcgaatct gctaaaccca aggaagcgga
    19201 ctacgttctc tatgatgacg atggccttga gctactcatt aaatccagcg ggagtaaaat
    19261 ctggcagttt cgctacattc gccctgtcac caagacacga gcaaagaaaa gcataggccc
    19321 ttacccgtca gttacccttg ccgatgccag aaactaccgt gcagagtccc gctcactcct
    19381 agctaaacag attgagccgc aggaacatca gcaagaacaa ttgcgtagtt cgctggaagc
    19441 caaaaccaat actttccagc tcgtagctga acgttggtgg aatgtgaaga aagccagtgt
    19501 gacagaggac tatgcggaag atatctggcg ctctcttgaa agagatgtct ttcctgcgat
    19561 tggcgacgtt agcgttacag atattaaagc tcatacactg gttcaggccg tacaaccggt
    19621 tcaggccaga ggagcactgg aaacggttcg tcgccagtgt caacgtatta atgaggtcat
    19681 gatctatgcc cagaacacag ggctgattga tgctgttccc agcgttaata tcggtaaagc
    19741 cttcgaaaag cctcagaaaa agaacatgcc cagcattcga ccggatcagc tatctcaact
    19801 gatgcagaca atgcgaacag ccagcattag cctttccaca cgctgcctgt tcatgtggca
    19861 acttcttact attacccgcc ctgccgaagc ggctgaagct cgctgggaag aagtagacat
    19921 agaagcgcaa gagtggaaga ttcccgcagc acgcatgaaa atgaaccgcg accatactgt
    19981 tccattgtca gatgaagcaa tagctgtgct ggagatgatg aaaccgctaa gcggcaaccg
    20041 agaatttatc tttcctagcc gtatcaagcc caaccagcct atgaacagtc agactgttaa
    20101 cgcctcacta aaacgcgcag gttttggtgg tgtgcttgtt tcgcatggtc tgagatcaat
    20161 tgccagtacg gctcttaatg aacaaggctt tccgcctgat gccattgagg cagcattggc
    20221 ccatgtggat aagaatgagg ttcgtcgtgc ttataaccgc agcgattacc tggaacagcg
    20281 tcgcccgatg atgcaatggt gggcaaattt tgtgatggca gcggatcgtg gaagcatgat
    20341 tgaaggtggg ataaaaggaa tgaagctagt tgggtgacta aatagctgcg cggcatagtg
    20401 tcccactttc agccagccct tgcaggttaa gccaccagac agtaaacggc aggtgtcgcg
    20461 ttattaggcg cagctattta gtagtaatat taggattggt gattctgcac tccagaagtt
    20521 cggtaatgct gccaacttac tgatttag
//
LOCUS       DAJTNM010000070        19799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTNM010000070 DAJTNM010000000
VERSION     DAJTNM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.070"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(47..139)
                     /locus_tag="OO172_002550"
     tRNA            complement(47..139)
                     /locus_tag="OO172_002550"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Ser,seq:gct)
     gene            complement(455..640)
                     /gene="csrA"
                     /locus_tag="OO172_002551"
     CDS             complement(455..640)
                     /gene="csrA"
                     /locus_tag="OO172_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5512694.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(875..3505)
                     /gene="alaS"
                     /locus_tag="OO172_002552"
     CDS             complement(875..3505)
                     /gene="alaS"
                     /locus_tag="OO172_002552"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5512695.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3633..4133)
                     /gene="recX"
                     /locus_tag="OO172_002553"
     CDS             complement(3633..4133)
                     /gene="recX"
                     /locus_tag="OO172_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5512696.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4376..5437)
                     /gene="recA"
                     /locus_tag="OO172_002554"
     CDS             complement(4376..5437)
                     /gene="recA"
                     /locus_tag="OO172_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5512697.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="OO172_002555"
     CDS             complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="OO172_002555"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5512698.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="OO172_002556"
     CDS             complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="OO172_002556"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5512699.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7500..8063
                     /gene="srlA"
                     /locus_tag="OO172_002557"
                     /gene_synonym="gutA"
     CDS             7500..8063
                     /gene="srlA"
                     /locus_tag="OO172_002557"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR5512700.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8060..9019
                     /gene="srlE"
                     /locus_tag="OO172_002558"
                     /gene_synonym="gutE"
     CDS             8060..9019
                     /gene="srlE"
                     /locus_tag="OO172_002558"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5512701.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9030..9401
                     /gene="srlB"
                     /locus_tag="OO172_002559"
                     /gene_synonym="gutB"
     CDS             9030..9401
                     /gene="srlB"
                     /locus_tag="OO172_002559"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5512702.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9405..10184
                     /gene="srlD"
                     /locus_tag="OO172_002560"
     CDS             9405..10184
                     /gene="srlD"
                     /locus_tag="OO172_002560"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5512703.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10289..10648
                     /gene="gutM"
                     /locus_tag="OO172_002561"
     CDS             10289..10648
                     /gene="gutM"
                     /locus_tag="OO172_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5512704.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10715..11488
                     /gene="srlR"
                     /locus_tag="OO172_002562"
                     /gene_synonym="gutR"
     CDS             10715..11488
                     /gene="srlR"
                     /locus_tag="OO172_002562"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5512705.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11481..12446
                     /gene="gutQ"
                     /locus_tag="OO172_002563"
     CDS             11481..12446
                     /gene="gutQ"
                     /locus_tag="OO172_002563"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5512706.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSSTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12443..13957)
                     /gene="norR"
                     /locus_tag="OO172_002564"
     CDS             complement(12443..13957)
                     /gene="norR"
                     /locus_tag="OO172_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5512707.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGNDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14144..15583
                     /gene="norV"
                     /locus_tag="OO172_002565"
     CDS             14144..15583
                     /gene="norV"
                     /locus_tag="OO172_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5512708.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15580..16713
                     /gene="norW"
                     /locus_tag="OO172_002566"
     CDS             15580..16713
                     /gene="norW"
                     /locus_tag="OO172_002566"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5512709.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16841..19093)
                     /gene="hypF"
                     /locus_tag="OO172_002567"
     CDS             complement(16841..19093)
                     /gene="hypF"
                     /locus_tag="OO172_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5512710.1"
                     /translation="MAKNTFCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19246..19773)
                     /gene="hydN"
                     /locus_tag="OO172_002568"
     CDS             complement(19246..19773)
                     /gene="hydN"
                     /locus_tag="OO172_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5512711.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISCDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
BASE COUNT         4658 a         5515 c         5044 g         4582 t
ORIGIN      
        1 tcggttcgta gccgagtact ctatccagct gagctacgga tgcaaatggc ggtgaggcgg
       61 ggattcgaac cccggatgca gcttttgacc gcatactccc ttagcagggg agcgccttca
      121 gcctctcggc cacctcacca cacgcctctt acgagtgctt cgaagaactt gtttatcgct
      181 catcgtcgct gcgtggcgca catattactt tctgagactt ataagtcaaa caatttttcc
      241 cacactttta tcgtttgcac acttcacgct caattagtct gtaaaaacgt caaaaagagt
      301 gttttatcta cagaaaaacg gaggtctggc agatagcagt aatgcaaaaa aatggagact
      361 taagttgaat gaacgggagt aaagcgaaaa gactatagag tgaaggagaa attttgaggg
      421 tgcgtctcac cgataaagat gagacgcgga aagattagta actggactgc tgggattttt
      481 cagcctggat acgctggtag atctcttcac ggtgaacaga aacttccttc ggggcattta
      541 cgccaatacg tacctggttg ccctttaccc ctaaaactgt cacggtgacc tcatccccaa
      601 tcatgagggt ctcaccaact cgacgagtca gaatcagcat tctttgctcc ttgaaagatt
      661 aaaagagtcg ggtctctctg tatcccggca ttatccatca tataacgcca aaaagtaagc
      721 aacgacaaac acattacatg taagcagtca tggcattaca ttctgttaaa cctaagttta
      781 gccgatatac acaacttcaa cctgacttta tcgttgtcga tagcgttaat gcgaatgccg
      841 tgaagcgagt ccacggcatt gcctgacgct tatattattg caatttcgcg ctgacccagc
      901 ctttcacact ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca
      961 tgtcaggacg tccaccaccc ttgccgccca cctgctgagc gaccatacca atcagttccc
     1021 ctgctttcac acgatctgtg acgtccttag atacgcctgc aatcagagaa accttacctt
     1081 cggctaccgt tgccagcacg ataattgtcg accccagctg attttttaaa tcgtcaacca
     1141 tggtacgcaa cattttcggc tcaacaccgc taagctcgct aaccaacagc ttaacaccat
     1201 taacatcaat tgccttactg gaaagatttg cgctctcctg tgcggcagct tgttctttaa
     1261 gctgttgtag ttctttttct aactgacgcg tacgttccag tactgagcgc actttatccg
     1321 ccagattatt gctatcgcct ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg
     1381 catgaacggt ggtgatagcg ccttctccgg ttaccgcttc gatacgacga acgcctgcag
     1441 cagtacccga ttcagagatg atgcggaaca gaccaatatc accagtgcgg ctggcgtgag
     1501 taccgccaca cagctcggtg gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat
     1561 acttctcgcc gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga ttcatgatgt
     1621 tggtttcgat cggcaaattg cgacgaatct gtgcgttcac caggtcttcg accgcacgaa
     1681 tctcttccgg tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa
     1741 ccagtgaacc tttctgcgat acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca
     1801 ggtgcgttgc ggagtgattc agacgaatac gggcgcgacg agcctcatca atatcagcct
     1861 gcacagcgtc gcccactttc agagaacccg cagcaagttt accgatgtga ccaatcgcct
     1921 ggccgtattt ctgcgtatct tccaccgcga aggagaagtt agcgcctttc agttcgcctt
     1981 tatcaccaac ctgaccgccg gattccgcat agaatggcgt ttgatccagc acgaccacag
     2041 cttcctggcc tgcattgatg gcatcaaccg ctttaccatc aacaaacagc gcggtcactt
     2101 tgccgttcag ttccagatgg tcatagcctt taaattcaga tgcactatca acacggatca
     2161 ttgcgttgta atcggcacca aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca
     2221 ttgctgcttc aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg
     2281 tcaggtcaac cgggaagcca taggtgtcgt acagacggaa agccgtttca ccatccagcg
     2341 tatcaccaga aagttttgcc agctcttcat ccagcaacgc cagaccgcgc tccagagtac
     2401 gggcaaactg ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca
     2461 ggtcttcacc cgcagagccc ataacgtcga tcagcggacc aaccagtttg tagaagaagg
     2521 tttctttcgc gccgagcata ttgccgtgac gcactgcgcg acgaatgata cgacgcagta
     2581 cataaccacg gttttcattg gacggcatta cgccatccgc gatcaggaac gcacaagaac
     2641 gaatgtggtc agcgattacg cgcagcgatt tattgctcag atcggtcgcg ccagtgactt
     2701 tcgctaccgc ctggatcaac gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt
     2761 gcagcaccgc agcaatacgc tccagaccca taccggtatc tacagacggc ttcggcagcg
     2821 gttccatcgt gccatctgcc tggcggttga actgcatgaa gacgatgttc cagatctcaa
     2881 tgtagcggtc gccgtcttct tccggacttc ccggagggcc accccaaatg tggtcgccgt
     2941 gatcgtagaa gatttcggtg cacgggccgc acggaccagt gtcacccatc tgccagaagt
     3001 tgtcagatgc gtatggcgca cctttgttat cgccgatgcg aataatacgt tcgcgcggga
     3061 tcccgacttc tttttcccag atttcgtagg cttcgtcgtc gctttcatag acggtaaccc
     3121 acagacgctc tttcggcagg gcaaaccatt tttcgctggt cagcagttcc catgcaaact
     3181 gaatggcatc gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt tcgaagaagg
     3241 tatggtgacg cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca
     3301 cgcagcgttg ggaagtggta gcgcgggaat aattacgctt gtcgagccca aggaacacat
     3361 ccttgaactg gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta tggggtacca
     3421 gggagctgct ggcaactacc tgatgtccct tactatggaa aaagtcgaga aacgcctgac
     3481 ggatctcagc ggtgctcttg ctcataatta tcctgaaatc aagctaacga aatatcgcca
     3541 ccagctccag cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat aagataagtt
     3601 ttcttgactg ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc
     3661 tggatatctt ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct
     3721 gaaaagacag ttggcaaagg ttcaccatat tttcgcgtcg cctgatcgcg cgccagtgcg
     3781 caccagtcga tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc
     3841 tgattcagtt cctgacgaat acgtgcaggt ccataacctt tgcggctacg gctggcgata
     3901 aagcgcgcaa caaatcggct gtcatcgaga tagccatgtt catggcacca ggcaataacg
     3961 cgctcgtaat cttctgccgt agcatcaatc tcttctgggc catttttgcc cataatcggt
     4021 gccgcgagtt tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc
     4081 gcacgatcca acaggcgagc atatgccggg cgacgggatg ttgattctgt catggcatat
     4141 ccttacaact taaaaaagca aaagggccgc agatgcgacc caggttttgc tgataaacag
     4201 atttaatagt caatgagtat gcagaatgtt cccgcaggcc caattcgtta gaggatcgag
     4261 gtgagagtaa tttttcacgc cagtgctggc ctgttcccct caccctaacc ctctccccaa
     4321 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
     4381 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4441 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4501 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4561 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4621 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
     4681 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4741 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4801 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     4861 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4921 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     4981 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     5041 gggcgtcaca gatttccagt gcctgctcgc cggtatccgg ctgggagcac agcaggttat
     5101 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5161 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
     5221 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5281 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5341 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5401 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5461 ataccggata gtcaatatgt tctgttgaaa caattatact gtatgctcat acagtatcaa
     5521 gtgttttgta gaaattgttg ccacaaggtc tgcaatacat acgcagtagc ctgacgacgc
     5581 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
     5641 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5701 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5761 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     5821 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
     5881 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
     5941 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     6001 tcactgtcag tcatcacaac ctctgtcagt caaaaattta tgccggacaa gatagcactt
     6061 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6121 tagccagagg gaagctcacg cccccctctt gtaaatagct actgtactcg cgccagcgcc
     6181 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt gtaacgggtg
     6241 atggtgtaga agttcggcag accgtaccag tactggtagc cggtgccaac atccagacgc
     6301 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgcggcggca
     6361 agctgcgaaa tgctgtactt agttttgaaa ccatttggca agcctggagc ctgaccgttt
     6421 gccattatcg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccacg
     6481 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6541 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6601 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcagcaggaa ggtttccagc
     6661 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6721 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6781 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6841 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6901 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     6961 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
     7021 cgatcgaaac cgtgtttgtt caccattttg tcgatgaact gctgggcatt cgggttatta
     7081 gcgaaatcgc cgcccatctg catcacattg tgctgcggct caagcaggaa gccgccagac
     7141 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7201 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7261 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7321 ctgcccgata cggactttac ataactcaac tcattcccct cgctatactt ttactcaaac
     7381 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaacctttca
     7441 acctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7501 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7561 aggtgtttac cggaatggtg accggcattc ttccgctgtt gattagcctg ctggttatca
     7621 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7681 ccggtaaccc tgtttcccgt tacctactgt taccgtgcat tggcacgttt gtcttttgca
     7741 atccgatgac cttaagccta ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7801 cggcggcctc ttatagctgc cactcaatga atggcctctt cccccatatc aaccctggcg
     7861 aactgtttgt ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc
     7921 cactggcggt gagttatctg ctggttggtc tggtcaccaa tttcttccgc ggctgggtga
     7981 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
     8041 acctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
     8101 cggcccgctt gagctggaag ctacgccggg caaaaaaatc gtctatatca ccgccggtac
     8161 ccggcctgcg attgttgaca aactggcaca acttactggc tggcaggcta ttgacggatt
     8221 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8281 attacgctgc ggcatctatc cgaaacgacg tattcccacc attaatatcc actcgacggg
     8341 caagtccggt ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8401 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8461 tgacaccagt aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8521 catggggtcc gccgttgccg tgttgtttca gtctggtcgt gacaccatcg acactgtatt
     8581 aaaaaccatt ctgccgttta tggcattcgt ctcggcgctc attggcatca ttatggcttc
     8641 tggccttggt gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     8701 ggtcatgctg gcgctcatct gctccttccc actgctttca cctttcctcg gcccaggcgc
     8761 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     8821 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     8881 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     8941 tgtactggtg agccgctttt taaccggcgc gccgactgta ctgatcgcct ggtttgtctc
     9001 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9061 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9121 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9181 cactccatcc cggtttgcaa ttttcactcg ggcagcatcg ctatccggtg accgctgttg
     9241 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9301 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9361 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9421 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9481 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9541 ttaacgccga atatggtgaa ggtatggcgt acggttttgg tgctgacgcc actagcgagc
     9601 aaagcgttct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9661 tctatagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9721 accgttcgct acaagtgaat ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt
     9781 tgatgatccg cgacgggatt cagggtcgca ttattcagat caactcgaaa tccggcaaag
     9841 tgggcagcaa acacaactct ggctacagcg cagcgaaatt tggtggcgtc gggctgactc
     9901 aatcactggc gctggatctg gcggagtacg gcattacggt gcattcactg atgctcggta
     9961 acctgctgaa atcgccgatg ttccagtcac tgttgccaca atacgcgacc aagctgggta
    10021 tcaaaccgga tcaagtcgag cagtattaca tcgacaaagt accgctcaaa cgcggctgcg
    10081 attatcaaga tgtgctgaat atgctgctgt tctacgccag tcctaaggcg tcgtactgca
    10141 ccggacagtc gatcaatgtc accggcggtc aggtgatgtt ctgatcaaca gcggagatcc
    10201 gttaaggatc tccgtgagac tatagaatgc ctgatgcgct acgctcatca ggcatacagg
    10261 acttccgcca ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat
    10321 cgcctggtgt gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt
    10381 cgacacacta tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc actttaaacc
    10441 gcgggtcgtg gtcgccatcg cgctggacga tcagcagtgc atcgtcgaca ccttgtttat
    10501 gaaaggactg actgtcttcg cccgaccgca aaaaattccc gcaattaccg gcatgcatgc
    10561 gggtgattta cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc
    10621 attggcgctt aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt
    10681 atcattttga aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc
    10741 cattctggag tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt
    10801 tgacaccaca ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg ccggaaccgt
    10861 cattcgtact tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca
    10921 taaaacgctc atcaacaccc acaagaaaga gctgattgca gaagctgccg ttagttttat
    10981 ccatgatggc gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggttcccct
    11041 gctctcgcgc tttaataaca tcacggtgat gaccaacagc ctgcatatcg tcaatgcgct
    11101 atccgaactg gataacgaac aaactatcct gatgccaggc ggaacatttc gcaaaaaatc
    11161 ggcctcattt cacgggcaac tggcagagaa tgccttcgag catttcacct tcgataaatt
    11221 gtttatgggc accgacggca tcgatctcaa tgcgggcgta accacgttca acgaggttta
    11281 taccgtcagt aaggcaatgt gcaatgccgc gcgcgaagtg attttgatgg cggactcatc
    11341 aaagtttggc cgtaaaagcc ccaacgtagt ttgcagtctt gaaagcgtcg ataagctaat
    11401 taccgacgca ggtatcgatc cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt
    11461 gatcataacc ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa
    11521 tgctggagtt gcaggaagca agccgtttac cggaacgtct gggcgatgat tttgttcgcg
    11581 ccgccaatat catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg
    11641 gccacattgg taagaaaatc gccgcaacac ttgccagtac cggcacaccg gctttttttg
    11701 tccatccggc agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc
    11761 tgtttatctc ttactccggt ggcgcgaagg aactggatct gattattccg cgtctggaag
    11821 ataaatctat cgcgctgctg gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg
    11881 cgaaagcggt gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga
    11941 cctccagcac cgtcaatacc ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg
    12001 cgcgcggttt taatgaagaa gattttgccc gctcccaccc agctggggca ctgggcgctc
    12061 gcttgctgaa taaagtgcat catctgatgc gtcgtgatga cgctatccca caggtagcgt
    12121 taaccgccag cgtgatggat gcgatgctgg aactcagcag caccggtctg gggctggtgg
    12181 cggtatgtga cgctcaacaa caggtacaag gcgtctttac cgacggcgat ttacgtcgct
    12241 ggctggtagg cggcggcgca ctcaccacgc cagttaatga agcgatgacg acaggcggca
    12301 ccacattaca ggcacaaagt cgcgccatcg acgccaaaga agttctgatg aaacgcaaaa
    12361 tcactgccgc accagtggta gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg
    12421 atttctatca ggccgggatt atttaatcct tcaatcccag acgtttcgcc agccgatgca
    12481 ggttggcgac gtcagtttcc agcattcgcg cacaggctgc ccagttgtga tgattttgtg
    12541 ccagtgcctg gcgaatagtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct
    12601 taacaacggg caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat
    12661 gttgcgcctc aagaatcact tcatcgccgt tgcgggtggc tctcgccagc actaccgccc
    12721 gatgaatagc atgttccagt tcgcgcacgt ttcccggaaa actgtagtgt tgcagtaaat
    12781 ttcgcgcccc ggcacttaat accacgcggg agagcccctg ccgcaaacga cactgctcgc
    12841 agaaataccc cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcaccgaaa
    12901 gtggaaacac gctcaggcga tgaaacaaat cggcgcggaa tcgccctgcc agcacctctt
    12961 cgcgtaagtc gcggttagtc gccgccagca cgcgcacatc gacccgcaaa ctacggtcat
    13021 tgccaacgcg ctgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca
    13081 acgacaactc gccgatctca tcgagaaaca gagtaccgtt atccgccatt tcaaacttcc
    13141 cgctgcggtt actgatagcg ccagtaaacg ctcctttcac atgcccgaac aactcacttt
    13201 ccgccacact ttccggcagt gcggcacagt tgagatagac cagcggattc accgcccgtg
    13261 gcgaggcttc atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctga
    13321 tcagaacgtt gagatcggac gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc
    13381 caggggacaa gccaatcatc tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg
    13441 gcagcatatt ctggctttcc agttgttcaa tcagcaacgc attgcttaac gctcccgccg
    13501 ccagcgcggc aatcagccgt aactcttcgt cgctgaaaac atcgaactga tcgggctgca
    13561 tcccgtcgag cgtcagtgcg ccgatcaggt tttgtccgac aaacaatggc agaccaacgc
    13621 aggcgtgaac cttcagactc tcctgcccag gaatcaaacc gtcataggga tcgggcaatt
    13681 cgctgtctgc gggaaagcgc accacatccc cggcgcgggc aatcgcttcc agccgtggat
    13741 gcccttccag cgcaaagcgt ctaccgagta catcctttgc cagaccgtcg atggcaagcg
    13801 gaataaactg ccgcgaatcg taacgtagca acgcagacgc atcgcactcc agcacctgac
    13861 gtagcgtggt gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga
    13921 tggcgatatt cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac
    13981 tatagatatt gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatgagc
    14041 ataattttat ttatagagta aaaacaatca gataagaaac tggcacgcaa tctgcaatta
    14101 gcaagacatc tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa
    14161 aaataacatt cattgggtgg gtcaacgtga ctgggaagtg cgtgattttc acggcacgga
    14221 atataaaacg ctgcgcggca gcagctacaa cagctacctc atccgcgaag aaaaaaacgt
    14281 gctgatcgac accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga
    14341 aatcgatctg gcggatatcg attacatcgt gattaaccat gcagaagagg accacgccgg
    14401 ggcgctgacc gaactgatgg cgcaaattcc cgatacgccg atctactgta ctgccaacgc
    14461 tatcgactcg ataaatggtc atcaccatca tccggagtgg aattttaatg tggtgaaaac
    14521 tggcgacacg ctggatatcg gcaacggcaa acagctcatt tttgtcgaaa caccaatgct
    14581 gcactggccg gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga
    14641 tgctttcggt cagcactact gcgacgagca tctgttcaac gatgaagtgg atcagacgga
    14701 gcttttcgag cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt
    14761 aacgccgaaa attaccgaga tcctgggctt taacttacca gtcgatatga tagcgacctc
    14821 ccacggcgtg gtctggcgcg ataacccgac gcaaattgtc gagctgtacc tgaagtgggc
    14881 cgctgattat caggaagaca gaatcaccat tttctacgac accatgtcga ataacacccg
    14941 catgatggct gacgctatcg cccagggaat tgcggaaacc gacccacgcg tggcggtgaa
    15001 aattttcaac gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa
    15061 aggcgtgctg gtcggcactt cgacgatgaa taacgtgatg atgccgaaaa tcgccgggct
    15121 ggtggaggag atgaccggat tacgcttccg taacaaacgc gccagtgctt tcggatctca
    15181 cggctggagc ggcggtgcgg tggatcgcct ttccacgcgc ctgcaggatg cgggtttcga
    15241 aatgtcgctc agcctgaaag ccaaatggcg accagaccag gacgctctgg agttatgccg
    15301 tgaacacggt cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt
    15361 gaatacggta gttaaagaag aaacctctgc cacgacgacg gctgacctcg gcccacgaat
    15421 gcagtgcagc gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt
    15481 tgcgccagga acgccgtgga gtgaagtccc ggataacttc ctctgcccgg aatgctccct
    15541 cggcaaagac gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc
    15601 atcggttcgg gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccact
    15661 attccattaa ccctgattgc cgccgacagc atagatgagt acaacaaacc cgacctcagc
    15721 catgttatca gtcaggggca acgtgccgac gaccttaccc gccagacggc gggtgaattt
    15781 gccgggcagt ttaatctgcg cctgtttccg cacacctggg tgacggatat cgatgccgaa
    15841 gcccatgtgg tgaaaagcca gaataatcag tggcaatacg acaagttagt gctggcaacc
    15901 ggtgccagcg cctttgtccc gcctgtgcct gggcgtgagt taatgctgac gttaaatagt
    15961 cagcaagagt atcgcgcctg tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc
    16021 ggcggtggtt tgattggtag cgaactggcg atggattttt gtcgtgcagg caaagcggtc
    16081 acgctaatcg acaacgctgc cagtattctg gcgtcattaa tgccaccaga agtaagcagc
    16141 cgcttgcagc atcggttgac ggagatgggc gttcatctgc tgttgaaatc tcagttgcag
    16201 gggctggaaa aaacggattc tggcattctg gcaacgctgg accaccagcg tagtatcgaa
    16261 gtggatgcgg taattgctgc caccggactg cgcccggaaa cagccctggc acgacgcgcc
    16321 gggctgacga ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgccgat
    16381 atttacgcgc tgggcgattg cgcggaaatt aacggtcagg tattgccgtt cctgcagccg
    16441 attcaactta gcgggatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa
    16501 ctaccggcga tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa
    16561 acccagcgtc aggatttacg ctggcagatt aataccgaac gccagggaat ggtggcgcgc
    16621 ggcgttgacg atgctgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag
    16681 gcatttgtat tgttgaaaac gttgccggtg taggtgggct actgtgccta aaatgtcgga
    16741 tgcgacgctg gcgcgtctta tccgacctac ggggacgcat gtgtaggccg gataaggcat
    16801 ttacgccgca tccggcaagg gtgtccaaat gcaacacgct ttatccgttc tggacttcac
    16861 ccgctaacca acgcgccgca gcaataaccc cctgccccag agacaaaccg ccatcacccg
    16921 ccggtaaact ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc agacgtgcac
    16981 gcagcaaacg gttatgaata accccgccgc taaataccag cgtagtgata ccacgcatcg
    17041 tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg
    17101 cgcgttgatt aaccggtgcc tgccagttca gccactgctg ccagaaagtg gcgagatcca
    17161 gttgattgtc cacccgcggc atcgtcaccg gatgcgtcac tccgtcgcat gaggctgcga
    17221 gcgcctccag agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca
    17281 gtgccgccgc cactgcatcg aacaaccgcc cacacgatga tgccagcggc gcgttaattc
    17341 cacgctcaat ggcccgcgcc agtacgctcc agttttgctg ttgcacactt gccgtttcag
    17401 ggtaattctg ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct
    17461 gcttcgccgc caaatcgcca cccggaagcg ccactgcagg caagccgccc aggtgctcac
    17521 attcgcgata gttcacccgc aggcactcgc cgccccacaa agcgccattc tcccccatac
    17581 cgataccgtc gagcgtcaaa gcaatgacat caccgccatc cagcggccac tgatgctctg
    17641 ccagacacgc cgctgcatgg gcatgatgat gcagtaccgt ttgcgtcggc agattcattt
    17701 cgctcgccca ctggctggag acatagcccg gatgcacatc atgcacaatg tattgcggag
    17761 tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat
    17821 cgtcacttaa atcgcccaga tgctgactca acaccacttg ttcaccgcgc accaggcaga
    17881 aggtattttt cagatccgcg ccaagacaca gcacaggcgg aacattttta aagcccggag
    17941 gcaaaaccag cgcatccggc acataccccc gcgaacggcg cagcatttcg ccgctttcgc
    18001 gcaccactga atcatccatc cgctgcacga tgtcgcggtt atgtagcaag aatccgtcgg
    18061 caatgccctg caaatccgcc agcgcctgtt cgttgctgat agctggtggt ttaccgctca
    18121 ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg
    18181 ggttcgcagg caacatcacc ccgacttcgt taaggccagg ggcgatatca tcacaaagct
    18241 caggtacgta ttttttatcc accagcacaa tcggcgcagc gggcgtggta agcaactggc
    18301 gcgcagcgtc tggtaaactt tctgccactg gcaacatgac tgccagcggt ttcgccgggc
    18361 gatgtttgcg ctcccgcagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat
    18421 gaaatccgcc aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagctg
    18481 cctgtaatgc cgcctcttgt tctgcatgtt caccatgact tacccattca agatgcgggc
    18541 cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcgcggtact
    18601 ctttgtcaca ggccggacat agcggaaacg ccgccatcac ggtaaacggg cggtcgtaag
    18661 gcatcgcgcg aataatggtg aaacgcgggc cgcagtgggt acagttgata aacggataac
    18721 gataacgccg ctcgcctggg gtattcattt cggcaaggca agcagggcaa gtggcggcat
    18781 ccgggacaat ttgcgtattc atggtgccgc cggtgctctg gcggatggtg aactcggtgg
    18841 gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc agtggcggac
    18901 agtgctgatg caattgaaca agaaacgttt ccggatcttc cagcagccgg acttctacgc
    18961 catcgccgtc attacataca tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa
    19021 acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaaaatg
    19081 tgttttttgc cattgagtta ttcccgccat catgaattgc gtaacccgcc ctgccggaca
    19141 cgacagcgtc gtatccggca gtctcaggtc ggcgataccg cccgctccgt attctacgaa
    19201 tatttccggg aattcctttg atgccagaac agttctgtaa gatttttaga acatcagcgc
    19261 cgtacggcgg cgtttttctg cgctcagttg ttcaagttta ttacgatcga cacaaatcag
    19321 cgcatgggtc gggcaagccg ccatacacgc cgggccgtct tcacgatggt tgcacaggtc
    19381 gcatttattg gcttcggctt tgtcagcccg tacattcaga cccgcgccgc tgttgcgaat
    19441 cacaggacgt accaccactt ccatcgcgcc atacgggcaa gccaccacgc aggttttgca
    19501 accaatgcaa cgttcctgca tcacatgaac aaacccttta tcacagctga tagcaccatt
    19561 cgggcagacg ttagcgcacg gtgcatcttc acactgacgg caaactgtcg ccgtggaaat
    19621 gttcacacct ttaatgacat ggatacgcgg taaaaatgtt tccggggtca gcgatgcaca
    19681 gtcctgattt tcctgatgag aaaccacgca cgccacttca caggtacggc aaccaataca
    19741 tttactcgcg tcagcaatga tgaaacggtt catcaaattc tccagcaatg acagttaat
//
LOCUS       DAJTNM010000071        19628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTNM010000071 DAJTNM010000000
VERSION     DAJTNM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.071"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..110
                     /locus_tag="OO172_002569"
     CDS             <1..110
                     /locus_tag="OO172_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCR5512712.1"
                     /translation="FTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(146..1102)
                     /locus_tag="OO172_002570"
     CDS             complement(146..1102)
                     /locus_tag="OO172_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5512713.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1102..2343)
                     /gene="clsB"
                     /locus_tag="OO172_002571"
     CDS             complement(1102..2343)
                     /gene="clsB"
                     /locus_tag="OO172_002571"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5512714.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2340..3101)
                     /locus_tag="OO172_002572"
     CDS             complement(2340..3101)
                     /locus_tag="OO172_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5512715.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3234..3644
                     /locus_tag="OO172_002573"
     CDS             3234..3644
                     /locus_tag="OO172_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5512716.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3606..4712)
                     /locus_tag="OO172_002574"
     CDS             complement(3606..4712)
                     /locus_tag="OO172_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5512717.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4723..5856)
                     /locus_tag="OO172_002575"
     CDS             complement(4723..5856)
                     /locus_tag="OO172_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5512718.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(5849..7585)
                     /locus_tag="OO172_002576"
     CDS             complement(5849..7585)
                     /locus_tag="OO172_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5512719.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7578..8573)
                     /gene="hlyD"
                     /locus_tag="OO172_002577"
     CDS             complement(7578..8573)
                     /gene="hlyD"
                     /locus_tag="OO172_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5512720.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8576..9247)
                     /gene="cecR"
                     /locus_tag="OO172_002578"
     CDS             complement(8576..9247)
                     /gene="cecR"
                     /locus_tag="OO172_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5512721.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9476..10663
                     /gene="rhlE"
                     /locus_tag="OO172_002579"
                     /pseudo
     CDS             9476..10663
                     /gene="rhlE"
                     /locus_tag="OO172_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(10870..11352)
                     /gene="ybiA"
                     /locus_tag="OO172_002580"
     CDS             complement(10870..11352)
                     /gene="ybiA"
                     /locus_tag="OO172_002580"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5512722.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            11472..13622
                     /gene="dinG"
                     /locus_tag="OO172_002581"
     CDS             11472..13622
                     /gene="dinG"
                     /locus_tag="OO172_002581"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5512723.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13650..14612
                     /gene="ybiB"
                     /locus_tag="OO172_002582"
     CDS             13650..14612
                     /gene="ybiB"
                     /locus_tag="OO172_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5512724.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            14753..15838
                     /gene="hcxB"
                     /locus_tag="OO172_002583"
     CDS             14753..15838
                     /gene="hcxB"
                     /locus_tag="OO172_002583"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5512725.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHKETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(16067..16327)
                     /locus_tag="OO172_002584"
     CDS             complement(16067..16327)
                     /locus_tag="OO172_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5512726.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16592..16858)
                     /gene="ybiI"
                     /locus_tag="OO172_002585"
     CDS             complement(16592..16858)
                     /gene="ybiI"
                     /locus_tag="OO172_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5512727.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(16932..17609)
                     /gene="ybiX"
                     /locus_tag="OO172_002586"
     CDS             complement(16932..17609)
                     /gene="ybiX"
                     /locus_tag="OO172_002586"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5512728.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLLLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(17651..19628)
                     /locus_tag="OO172_002587"
                     /pseudo
     CDS             complement(17651..19628)
                     /locus_tag="OO172_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4550 a         5179 c         5302 g         4597 t
ORIGIN      
        1 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
       61 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag tcgtaaatgc
      121 agatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcttct
      181 tcgcttggct ttccagtagc aggtaacaga tcagcgccag cagcagcggg ataaagtaat
      241 acaggacgcg ataagcgagc agggcggcga taatgctccc tttcgaggta tgctccccag
      301 ccagtagtgc gataaacacc gcttccagca caccgatccc cgccggaata tgcacgatga
      361 cgccagcaat actactaacc agtaacacgc ccagtacaaa gaaatagttc acgctttgac
      421 caagtaacag ccagataatc gcccccatta ccatccagtt aacgctggaa atcagcatct
      481 gagcgagggc gaatttccat gaaggcagca ccagcttttg ccctttgatg gtcatatggc
      541 ggtgcttcgc gaaagcgcaa aaccacaaat aaaccgcgat aatcatcagt aagccaatgc
      601 cgagaatacg cagcgtagtt tgatcgacat accagtgatc cggcaactcc accacgcctg
      661 cggtaaagat aatccctgcc agtaaaatat agcccagcca gttggtggta atactgagcg
      721 aggaaatccg cgtaatagtg ctgcccggta accctagtcg ggagtacaaa cgatagcgca
      781 taccaatgcc gccgacccag gtactgagcg tcaggttgaa ggcgtagcag ataaacgaca
      841 ccagcatcac ctggcgcttc gccagtttgt gaccgcagta aaagcgggcg agcaggtcat
      901 agcagccgta aatcagatag ctgacgacca ccagcccgac cgcactaagc agcgcaacgc
      961 gattgtagtc gcggatgacc ttccagacct cttcccagtc cacttttttg gcgtagacca
     1021 ccagcaacac aatcaccgcg ataaaaaaca gccaggtgag gatcttcttt gctaagcgcc
     1081 agcgcgggtg tgatttactc atcagggttt tacccccgtg ttttcagttt ctacccgatc
     1141 ctgcgtttcc attgtcggtt gtgcgggggg atcaacctgc gtcagacgtg gtgtgtgtgc
     1201 cggaagccag ccaaccagcg ccgggaagtg gcgtaaaaag tggaacgcca gcacgctttt
     1261 ggtcaggttc caccaggtgc gtttgggcag catggtttca tccacctgct gacaatctgc
     1321 ggcaataatg ccgttcagat tatcgcgcag cgtctggtta aaatgacgat cgtggatgat
     1381 gacatttgct tcgagattca gtgacaaact gagcggatcg agattactgg accctactgt
     1441 cgcccagtga tcgtccatca atgccacttt gccgtggagc gggcggcggc ggtactcaaa
     1501 aacctgaacg ccgcctttaa ccagatagtt atacagcaag cgcgcaccga ctctgacaat
     1561 cggcatatcc ggttcgccct gaatgatcag tttgatccgc accccgcgcc gtgccgcttt
     1621 acgcaaggcg tgtaaaaatc gatagccggg gaagaagtag gcgttggcga taatcacttc
     1681 ccgccgcgcc tgagtgagca ttttcagata atggcgttca atatcatcgc gatgttcttc
     1741 gttatcgcgc cagaccagca atacctgcgc ttctcccggc tggcggttct cttccgcttt
     1801 gtgatgacgt cgccaccagc gtcgtgccgc gctctgtcca ggcaggtttt ccagctcaaa
     1861 ctggagaata tcttcgacaa tcggcccttc aaggcgtacc gcgtaatcct gtttagcctc
     1921 tggaccgtag ctggacatat gctcggcgga gtaattcagc ccgccaataa aggcgatacg
     1981 cgcgtcgatc accacaattt tgcgatgcat ccggcgaaac acattggtgc gcataccaaa
     2041 aaggcgaggg cggggatcgt agtagcggaa cactacgcca gctgccgtca gttcattgac
     2101 aaactcatcg ctgagatccg gcgaaccgta gccatccagc aagacttccg ctttaacccc
     2161 gcgttgcgct gctgccagta gtgccgcatg cagctgtttg ccgacgttat cctcaaacca
     2221 gataaacgtt tcaagaatga tgcgttcttg tgcctcgcca atcgccttaa acaccgcggg
     2281 ataatattgc tcgccgtttt ccagcaactg gatcttattg ccttcgcgcc agctacattt
     2341 cataaatgaa tctccgcact taaaggggca tgatcagaaa ggtgtcgcca tgtccgcagc
     2401 ggcaacgcgg ttggcgcgct ggcgctggca tttttgacgt agatcctgtc cagtcgtagt
     2461 agaggaaatt gcaccggaaa cgtgcgcgcc gggcgtccgt gggcgcgggt aaaaatctca
     2521 tccagtccgg cctgcacttt taacggatga ttagcttttt gccgccagtc attgaaatca
     2581 cccgccacca ataccggttc gccgtccggt agctcattca cccattcggc gagcatcgca
     2641 agctgcgcct gacggtgcgc ctcacgcagg cccagatgta cgcacatcac atgaatcgct
     2701 tttccggtca tcggcggcac aatgcggcag tagagcacgc cgcgcttttc cgcaccatcg
     2761 accgaaacat cgcgattctc ataatgttca atgggataac gcgacagtac ggcgttgccg
     2821 tgatgccctt ccgggtatac ggcattgcga ccgtaggcaa aatcgctcca catagtgtcg
     2881 gcgagaaact cgtagtgcga ggtatcgggc cagttttcca catgcagcgg atgaacttcg
     2941 tgcgcgccca tcacttcctg caggcaaaca atatcggcgc tgacggtacg cacggcatcg
     3001 cgaagttccg gcaaaatgaa gcgtcggtta aacgcggtaa agcctttgtg aatgttgatg
     3061 gtgagcacct tgaacgaaaa ttgttgtgtt tgatcgggca taattttcct gtctttgcct
     3121 cttatctcat tgaaatagtg tagtcggcgt cacaaaaagg tgcggtctta cggaattttc
     3181 cgtaaagttc ggtactctga gtaagtagag ataaattctt caggagagaa gccatgaagt
     3241 ggcaacaacg tgttcgtgtc gcaacgggtc taagttgctg gcagattatg ttgcatttac
     3301 tggtagtggc gctgctggtg gtgggctgga tgagtaagac tctggttcac gtcggcgtgg
     3361 gattatgcgc actgtattgt gtcacggtag tgatgatgct ggtgtttcag cgccaccccg
     3421 agcaacgctg gcgtgaggtg gcagacgtgc tggaagagct gaccacgacc tggtattttg
     3481 gcgcagcgct gattgtgctg tggctgttgt cccgcgtact ggaaaacaac tttttgctgg
     3541 caattgcagg gctggcgatc cttgctggcc cggcggtagt gtctttgctg gcgaaagata
     3601 agaagttaca tcaccttacg tctaaacatc gcgtacgccg ctgaccctgt cgtggccgtt
     3661 atcaccagta gcggccacaa actattccac acaatatcca gactcgcatc cttcaaataa
     3721 atctgcttgg taatgtccgt aaagtggcga atagggttaa tccacgtcag attttgcagc
     3781 cacactggca tgttttccac cggcgaaacg taaccggaaa ggagaatggc gggcatcata
     3841 aaaacaaaca cgccgataaa cgcctgctgt tgtgtcgagc agagtgatga aatcaacaga
     3901 ccgaatccca ccagcgataa accgtaaatc accatcgtaa agtagaacag cgctagcgat
     3961 ccggcgaagg ggatttgata cgcccagata ccaatcgcca gcacaatggt ggcctggaag
     4021 gtggcgacaa ttaacgccgg tacggctttg ccgatgaaga tctgccaggt ggtgagcggc
     4081 gaaaccagta gctgatcgag cgtaccttgt tcacgttcgc gggcgacgga aagtgaagtg
     4141 acgatcatta cgccgatagt ggtgatcatg gcgatcagcg acggcaccac aaaccatttg
     4201 tagtcgagat tcgggttata ccagttgcgt accaccagct cgctgttgtt gggtttcggt
     4261 tttccttcca gcagctcctg ctgataattt ttgacgatct gttgcaggta gttggcggca
     4321 atttgcgcac tgttggagtt acgcccgtcg aggatcaact gcaacggcgc ggtctggaag
     4381 gtatccagtt tgcgcgagaa gtcagccggg aaacgcacca gtagtaacgc cttttgtgtg
     4441 tcgatggtcg ggcggatctc ctgtgggctt ttcagcagca gcacatgtgt aaaggcgctg
     4501 gcgcgggcaa aacgttgagt aagttccacc gaatgctcgc cgttatcttc atcgtagatg
     4561 gcgatggtgg cgttagtcac ttccagcgtg gcggcgaacg ggaacaggat cacctgaatt
     4621 agcacgggta aaatcagaat cgcgcgggtt tgtggttcgc gcagcaacga ctgcaactct
     4681 ttgcggatta acgtccataa gcgatgaaac atgctcttct ccctaatcca gccgacgttt
     4741 ggttttcagc cacgtcaggc cgataaacat caccgccgaa gcgatcaaaa acagcacgtt
     4801 taccaccagc accactggaa tattcccggc gaggaacagg ctttgcaggg tgctgacgaa
     4861 ataacgagcg ggaataatgt acgtcaccgc gcggatcacc gcgggcatac tgtcgatctg
     4921 aaaaataaag ccggaaagca taatcgacgg cagaaaagcg gcgttcaggg cgacctgagc
     4981 ggcattgaac tggttgcggg taatcgtgga aatcagcagc cccattccca gggtactgag
     5041 taaaaacagg ctggagataa aaaacagaat cagcagcgaa ccgcgatacg gcacgccgag
     5101 aataaacact gacaccagca tacacagcaa catcgccagc atcccgagaa agtaataagg
     5161 gatcagctta cacagcagca gttccgtgcg ggtaatctcc gtagagagca gagcctccat
     5221 ggtgccgcgt tcccattctc gcgccaccac cagcgaggtg agaatcgcgc cgatgaccgt
     5281 catgataatg gtcaccgcac ccgggataat aaagtgctgg cttatcgccg ccgggttaaa
     5341 ccagtagcgg gtttgtacat caataagcgg ttcaaaagtc tgcccgttgt cctccgctcg
     5401 ctgcatttgc cagatctgcc agatcccttc gacatacccc tgcacaaagt tagcggtatt
     5461 cggctcactg ccgtcggtga tcacctgaat cggtgcggtg gcgttggcgc gctccatctg
     5521 ttcggcgaaa tccacgggaa taaccaccag accgcgaatt ttccccgact gcattttggc
     5581 gatcagttcc tgacggttat cgctgatggt ggcgtcgatg tagggcgaac cggtcatggt
     5641 gtgggtgaaa tccagcgccg cttcgctacg ctgttccagc aaaatcccga cccgcagctt
     5701 gctggagtcg aggttaatgc cgtaaccaaa aataaacagc agtagcagtg ggatcactac
     5761 cgcaatcagc cagctactcg gatcgcgaac gatctgccgc gtctctttga cgcacagcgc
     5821 ccgcacgcga tgccaggaaa ggatcgggtt actcattgct atgctcctta tcccagtcgt
     5881 ggatcaactg aataaaggct tgctccatgg tgggatcggg ttgctcatcg ttagctgact
     5941 gtgctttcaa atcgtccggc gtaccgctgg cgattaattt cccacggtac accaggccga
     6001 tgcggtcgca atattccgct tcatccataa agtgggtggt gaccatcacc gtgacgcctt
     6061 tctctaccat gctgttgatg tgcagccaaa attcacggcg ggtgaggggg tcaacgccgg
     6121 aagtcggttc gtcgagaaag agaatatccg gttcatgcat cagcgaacag gccagcgcca
     6181 gccgctgttt aaaacctaat ggcagttcat cggtggcgtg tgaggcaata cttttcaggc
     6241 caaatgcctc gctcatacgg gagatttttt cgttctgcgc ccgaccgcgt aagccataca
     6301 caccagagaa aaagcgtaaa ttctgttcga ccgtcaggtt gccgtagagc gaaaattttt
     6361 gcgccatata gccgagatgc tggcgcgctt taccggaact ctctttcaga tccatcccca
     6421 gcaccagcgc ctggccggaa gtcggcacca gcaaaccgca catcatctta aaggtggtcg
     6481 atttacccgc gccgtttggc cccagcaaac caaaaatctc cccacgttta acggcaaagt
     6541 tgacgtgatc ggtggcggca aaatcaccaa atttcttggt cagttctttc gcttcgatca
     6601 ccgtctcgcc gggtgtgcct tctaccgtat gcaatattgc acccagcggc gattctgagg
     6661 ttccggcacc gcccagcaaa tcaataaacg catcttcaaa acgcggcgta gtttcgttga
     6721 tgttgatttc cggcatcccg tcggcatggc gaatatcgtc tggtgtggcc tcttttttga
     6781 ggatcagacg taccgatttc ccctgaatca tgccgtcgct gacctgcggc agtttcaagg
     6841 cgcgttgcaa cagtttgcgg ttgccctcgt gtggactggt catcagaaag ctgcgtccgg
     6901 ccatggtttg tgtcagggct tttggttctc cctgatacag caactcgcct tcgttcatca
     6961 gtaacacgtc acggcactgc tcggcttcgt cgagatacga ggtactccag aggattaaca
     7021 tcccttcgcc cgccagctca tgcaccatct gccacagttc gcgccgtgag atagggtcaa
     7081 cgccgacgcc gggttcatcg agcagcaaca ctttcggttc gcccaccagg gtacaggcca
     7141 ggccgagttt ttgtttcatc ccaccggaga gcttgcccgc caggcgtccg gtaaacggcc
     7201 caagagacgt aaactccagc aggcgagcaa aagtttgctt acgtgcctcg ccggtgacgc
     7261 tgcgcaaatc cgcgtacaga ttgaggttct ccatcaccgt gagatcttca tacagaccaa
     7321 atttctgcgg catatagccg agcacggcgt gcagcgcgcc gtcgtttttg atcggatcaa
     7381 agccaatcac cgtggcactg ccgctgtcgg gtttcagtaa tcccgccaac atccgcatca
     7441 gcgtggtttt acctgcaccg tccggcccca ccaaccccgt cacataaccg gcgtgaatgg
     7501 tacaatcgag cggcgcgacg gcgggcttgt ccatgcccgg aaagcgtttt tccaggccgt
     7561 tcagcgtgat aacggcatca ttcatgtcct gcctcgtcac cgaattgtac cgtcactggc
     7621 attccctggc gtaacgcatc atcggcgtcg gtcaccacaa tacgcaggcg atagacgagg
     7681 tcggtacgca gatccggtgt ttcgacggtt ttcggggtaa attcagcagt cggcgaaacg
     7741 aaaccaatct gcccgtgata cggcttgtcc gggcgaccat cggtataaag cagcactttg
     7801 cgccccggct gggcctggtc aagattccgt tcatcaacat aagcgcgcac ccacaccgga
     7861 cgcgttagtg aaacggtaaa caccgtgcca ccttcattga gaaccgtgcc tggctctacc
     7921 gcgcgcgtta acagcgtgcc atcagacggt gctatcaacg ttgagtcctg taaattcagc
     7981 tccgcctgcg ccagttgcgc ctgcgcctgt tcgaggctgg ctttcgcctg agcgatatcc
     8041 tgttcacggt taccggaacg gtactgacgc aatttatcct gtgctgattt cagcgttgcc
     8101 tgtgcctggt cgcgcgagga gcgggcattt tccaggtcat ttgccgaaat ggtgcggctt
     8161 ttccacagtc cttgctggcg gttatagaag ttctgcgcat agtcataggc ggcttgcgcc
     8221 tgtttcaccg ctgcggcggc ctgagcgatt tcttcatcgc gatacccggc aagcatcaga
     8281 tcatactgcg cctgtgccac cgagacgccc gctttcgcct gcatcagggc aatctcatac
     8341 ggcttgtgat ccagttcgcc cagcacctgg cccgctttga tagcatcacc ttcgtccacc
     8401 gccagcgatt caacgcgccc cccaacacgg aaactaagat tcaccgtacg aatatccacg
     8461 ttgccataca gcgtcaggcc gttatcttgg cggctttgat accaccagta gcctccggca
     8521 accacggcgg caagtaccac taccgccagt ccgatcacga caggtttttt catcactaca
     8581 aactcctttg cgataatcct tgcaaaatca ggtcgatatg acaggtcacc gtctggttga
     8641 tcagttcggt cttttcttca tcgaacgcgg tccagccggt acgtaacaga attgtttctt
     8701 tgccaagacg gaacgccaga atctcaccaa tcaacgcatg ggaatgaagg atcatgcggg
     8761 tgtcattagc gtcgcagccg gtccaggcgg caatcaggcg tgtcaggtgg ctgtgtagcg
     8821 gactaatcac ctgttcgtgc accaggtggt aggctgccgt gggagagagc tgctcacggg
     8881 agataaactt gctgaggttg acggtgtcat cctgggtgag cagcttaatc atgttcctgc
     8941 aggcgcgaag gatcagttca cggatggcag cccgatcagg ctgtggttgg gcgaacaagc
     9001 gttcggcttc ctcggcatgc ggacggaact gctcgccaat aaaatcggca atccactggg
     9061 cgcaggcgag gtacaaatct tctttcgaac cgaagtagta ggtgatggca gcgatattct
     9121 gcccggcctg ggcggctatc tcgcgggtgg tggcgttcat tccatattca ccaaactgcg
     9181 ccagtgcggc agcaatcagc tgttttttcg cctgttcacc cttgattgtc atggcaggat
     9241 tattcatcgc acagtccatt cttaatcaaa tgattgatta agattatgac tccatatggg
     9301 agttgtccag tatggctaag aattttagct acgccagtca cagggataat ttatgcgctg
     9361 cgtcacaaaa actgctacac tccgctccct catgacattg tggtttttgt cattttcctt
     9421 ttcagtatct ccctgaaaac tacaccggta acggtcgggg cggttcggag tagttatgtc
     9481 tttcgattct ttgggtttaa gccctgatat cctgcgcgcc gttgccgagc agggttaccg
     9541 tgaacccacc cctattcagc agcaggcgat ccctgcggtg ctggaaggcc gcgacctgat
     9601 ggctagcgcc cagaccggca ccggcaaaac agcgggcttt acgctgccgc tgttgcaaca
     9661 cctgatcact cgccagccgc acgccaaagg gcgtcgtccg gtacgtgcgc tcattcttac
     9721 cccgacccgt gaactggcgg cacagattgg cgaaaacgtc cgtgattaca gcaaatacct
     9781 gaacattcgt tcgctggtgg tgtttggcgg tgtcagcatt aacccacaaa tgatgaagtt
     9841 gcgcggcggc gttgatgtgc tggtggcaac gccgggacgt ttgctggacc tggaacatca
     9901 aaatgcagtg aagctggatc aggttgaaat cctcgtcctc gatgaagctg accgcatgct
     9961 cgacatgggc tttatccacg atatccgtcg cgtgttaaca aaactgccag cgaaacgcca
    10021 gaacctgctg ttctctgcga ccttctctga cgatattaaa gccctggcgg aaaaactgtt
    10081 gcacaacccg ctggaaatcg aagtggcacg ccgcaatacc gcgtctgatc aggtgaccca
    10141 gcacgttcac tttgtcgata agaaacgcaa acgcgaattg ctgtcgcaca tgattggtaa
    10201 agggaactgg cagcaggtgc tggtgtttac ccgtaccaaa cacggcgcta accatctggc
    10261 tgagcagctc aataaagatg gcatccgtag tgtggcgatc cacgggaata aatcgcaagg
    10321 tgcgcgtacc cgtgcgctgg ctgattttaa atcgggtgac attcgcgtac tggtggcgac
    10381 cgatatcgct gcgcgcggtc tggatatcga agagttgccg cacgtcgtca actatgaact
    10441 gccaaacgta ccggaagatt atgtccaccg tatcgggcgt accggtcgtg cggctgctac
    10501 cggtgaagcg ttgtcgctgg tgtgtgttga tgaacacaaa ctgctgcgtg atatcgaaaa
    10561 actgctgaaa aaagagatcc cgcgcattgc gattccgggc tatgagccgg atccgtcaat
    10621 caaagccgaa ccgatccaga acggtcgcca gcaacgtggc ggcatcaggc tgatgaacaa
    10681 acgcaaaact gcctgatgcg ctacgcttat caggcctacg tggactctgc aatatattga
    10741 atttgcatgc ttttgtaggc cggataaggc gtttacgccg catccggcat ttcacaacaa
    10801 gcacttgtca gcaatttgag aacacgggaa aaaatttatc tgttttaccg cccataagcc
    10861 accgagacgt tacttctcta tagccaattg ttcgcgcaac tccattaaaa ggtagcccag
    10921 tcgattcttg cccttaccat taccaccgtc tccccagtaa gcatcgtttt ccgtatgctc
    10981 aaccagtttt gcgggcgcgg ttgccagcaa gagcacacgc agttctgcat gctgttcgaa
    11041 tttagcgcga agtgctttac gcatcacctg ttctttaacc gactcccagt ttttacgaag
    11101 tggcttggaa cgattacgcc ccatgcgagc ggcgaccatg ggggaagaaa cccgacggat
    11161 ctcttcgcgg tatttttcat caaggaattt ttgtgcctga aaatagtgtt cggaggtggg
    11221 ccaggtattc ccgtcaacct tgatgggcct ggcggcaaag ttagaaaaat caccgtagtc
    11281 atcgctggtg ctgtagaaat ttatgatggt gtcttgcatg acgtgttgga tcctttgtgc
    11341 tcgaacgggc attaaaccgc attatgttgg tggttattgc gagccgcttt ccagaaacag
    11401 aaaaaccatt acccctgaaa accgaaaaat gccacaatat tggctgttta tacagtattt
    11461 caggttttct catggcatta accgccgcgc ttaaagcgca aattgccgcc tggtataagg
    11521 cgcttcagga acagatcccc gactttattc cccgtgcgcc gcagcggcag atgattgcgg
    11581 acgtcgccaa aacgctggcc ggagaagaag ggcggcatct ggcgattgaa gcccccaccg
    11641 gcgttgggaa aacgctctcc tatttgatcc ccggcatcgc cattgcccgc gaagagcaaa
    11701 aaacgctggt ggtgagtacc gccaacgtgg cgttgcagga tcagatttac agcaaagatt
    11761 taccgctgct gaaaaagatc attcccgatc ttaaattcac tgccgctttc gggcgtgggc
    11821 gctacgtttg tccgcgtaat ctgacggcgc tcgccagcac tgaacccacg caacaggatc
    11881 tgctggcgtt tcttgacgac gaactgacgc caaataatca ggaagagcaa aaacgttgtg
    11941 cgaagctgaa gggcgatctc gacacttata aatgggatgg tctgcgcgat catacggata
    12001 tcgctataga tgatgatctc tggcgtcgtt tgagtaccga caaagccagc tgcctcaacc
    12061 gtaactgtta ctactatcgt gaatgcccgt tttttgttac tcgtcgggag attcaggaag
    12121 cggaagtggt ggtggcaaac catgcgctgg tgatggcggc gatggaaagc gaagccgtac
    12181 tgcctgaccc gaaaaatctg ctgctggtgc tggacgaagg tcatcacctg ccagatgtgg
    12241 cccgggatgc gctggagatg agcgctgaaa tcaccgcgcc ctggtatcga ctacagctgg
    12301 acttgttcac gaaactggtc gctacctgca tggagcagtt tcgcccgaag accatcccac
    12361 cgctggcgat ccctgaacgt ttgaatgcgc attgtgaaga gttgtatgag cttatcgcct
    12421 cgttaaacaa cattctcaat ctctacatgc ctgccgggca ggaggcagag caccgttttg
    12481 cgatgggcga actgcctgat gaattgctgg agatctgcca gcggctggca aaactcaccg
    12541 agatgctgcg tggtctggcg gagttatttc ttaacgattt aagtgagaaa accggcagcc
    12601 acgatattgt gcgtctgcat cggttgattt tacagatgaa ccgcgcgctg gggatgtttg
    12661 aggtgcaaag caaactctgg cggctggcgt ctctggcgca atcttccggt gcgccggtga
    12721 ccaaatgggc gacgcgggaa gagcgcgaag ggcagctaca cctctggttt cactgcgtgg
    12781 gaatacgtgt cagcgatcag ctggaaaggc tgctgtggcg cagtattccg cacattattg
    12841 tcacctccgc aaccttgcgt tcgctgaata gtttttcgcg tttgcaggag atgagtggtc
    12901 tgaaagagaa agcgggcgac cgttttgtgg cgctggattc cccctttaac cactgcgaac
    12961 agggcaaaat tgttattccc cggatgcgct ttgagccttc catcgacaac gaagagcaac
    13021 acattgccga aatggcggcc tttttccgtg aacaggtgga gagcaaaaaa catctcggta
    13081 tgttggtact gtttgccagc ggacgggcga tgcagcgctt tctcgactat gtgacggatt
    13141 tacgcctgat gttgctggtg cagggcgatc agccgcgtta tcgcttagtt gaactgcacc
    13201 gcaaacgcgt tgccaacggt gagcgtagcg tgctggtggg cttacagtca tttgccgaag
    13261 ggcttgattt gaaaggtgat ctgctcagcc aggtgcatat ccacaaaatc gcttttccgc
    13321 ccatcgacag cccggtggtg atcaccgaag gggaatggct gaaaagcctc aaccgctatc
    13381 cgtttgaggt gcaaagcctg ccgagcgcct cgtttaacct gattcagcag gttgggcgac
    13441 tgattcgaag ccacggttgc tggggcgaag tggttatcta cgacaaacgc ttgctgacaa
    13501 aaaattatgg caagcgacta ctggatgcat taccggtatt tccgatagag caaccggaag
    13561 tccctgaagg tatagttaaa aagaaagaaa aaacgaaatc cccacgccgt cggcggcgtt
    13621 aatgatgtga gtcaggtaag gagtcgtaaa tggactatcg caaaataatt aaagagatcg
    13681 ggcgcgggaa aaaccacgcg cgcgatttag accgggatac tgcgcgcggt ctgtatgctc
    13741 atatgctcaa tggtgaagtc cctgaccttg agttgggcgg cgtattgatt gcgctgcgta
    13801 tcaaagggga aggggaagca gagatgctcg gcttttacga agccatgcaa aatcacacca
    13861 tcaagctgac gccgcctgca ggcaagccga tgccgattgt catccccagt tacaacggcg
    13921 cgcgtaaaca ggctaacctg acgccattat tggcgattct gctgcataaa ctcggttttc
    13981 ctgtggtggt tcacggagtt agcgaagatc caactcgcgt gctgacagaa accattttcg
    14041 aactgatggg tattacacca acgttgcacg gcgggcaggc gcaggcgaag ctcgacgaac
    14101 atcaaccggt gtttatgcct gtcggcgcgt tttgcccgcc gctggaaaaa caactggcga
    14161 tgcgctggcg gatgggcgtg cgtaacagtg cacataccct ggcgaaactg gcgacgccat
    14221 ttgccgaagg tgaggcactg tgtctttcca gcgtttcgca tccggaatac attggacgcg
    14281 tcgcgaagtt ctttagcgat atcggcgggt gggcgttact aatgcatggt actgaaggcg
    14341 aagtgtatgc taatccgcag cgctgcccgc agatcaatct cattgaccgt gaagggatgc
    14401 gggtgctgta tgaaaaacag gaccctgctg gtagcgagtt actgccacaa gcaaaagatc
    14461 cggaaaccac ggcgcagtgg actgagcgtt gcctggctgg cagcgaagcg attcccgaat
    14521 cgctgaaaat ccagatggct tgctgcctgg tggctacggg tgaagcggca actatcagcg
    14581 atggcctggc gcgcgttaat caggcatttt aatttttatc tcagccggat gagccacgtc
    14641 atccggcttt taccccaccg caatatgaaa ttcctgcatc tttattgacc ttcccgcgcc
    14701 cggcgtgcag cataaaaaaa caacaaacac ataacataaa caggagttaa ccatggaaag
    14761 tggtcatcgc tttgatgctc agacgctgca cagttttatt caggctgtat ttcgtcagat
    14821 gggtagcgag gaacaagaag cgaaattagt tgccgatcat ttaatcgcgg caaacctggc
    14881 agggcatgat tctcatggta ttggcatgat cccaagctat gtgcgttcct ggagtcaggg
    14941 gcatctgcaa attaaccatc atgccaaaat cgttaaagag gcgggggcgg cagtcacgct
    15001 cgatggcgat cgcgcattcg gtcaggtcgc ggcacacgaa gcgatggcgc tggggattga
    15061 gaaagcgcat cagcacggta ttgctgccgt ggcgctacat aactcgcatc atatcggccg
    15121 tatcggttac tgggcggagc agtgttcagc ggcggggttt gtctctatcc actttgttag
    15181 cgtggtcggt attccaatgg tcgcgccgtt ccacggtcgc gacagccgct ttggcaccaa
    15241 tcccttctgt gtggttttcc ctcgtaaaga taatttcccg ctgttgcttg attacgccac
    15301 cagcgccatt gcatttggca aaacccgcgt cgcctggcat aaaggcgtcc ccgtgccgcc
    15361 aggttgcctg attgacgtta acggcgtgcc gacgaccaat ccggcggtaa tgcaggagtc
    15421 gccgttgggg tcgctgttga cctttgccga acataaaggc tacgcccttg cagcgatgtg
    15481 tgaaattctt ggcggggcgc tttctggcgg taaaacgacg cataaggaaa cgttgcaaac
    15541 cagccccgat gccattctta actgcatgac cactatcatc atcaacccgg aactgttcgg
    15601 cgcgccggat tgtaacgcgc agaccgaagc ctttgccgag tgggtgaaag cctcgccgca
    15661 tgatgacgat aagccgattt tgctaccggg cgagtgggaa gtgaacacgc gtcgcgaacg
    15721 gcaggagcag gggattccat tggatgcggg aagctggcag gccatttgtg atgcggcgcg
    15781 gcagattggt atgtcggaag agacgttgca ggctttctgt cagcagttag ccagctaaaa
    15841 aaaagcccgt ccagtggcgg acgggcaaac aagggtaaca taggatcaat gagggttaga
    15901 gcatatgcgt ctgtcggcaa acagacaggg aaatacttgt gctggacgta gcgtaaacgc
    15961 ctgatccgtc caacggttcg gattttgtag gcctgataag acgcggtagc gtcgcatcag
    16021 gcatctgttg ccggatgcgg cataaacgcc ttatccgtcc tacgaattac ttataaatta
    16081 ccgcagtacc gcttaattta ttgttgttgg tggcggaagt gatgctgtat ccactggcac
    16141 cggctgccgc cgctttctca gccagtttcg cttccagggc atcgagggtg gatgcgccat
    16201 cggcagaaac cacgccgatt ttattcatgt tctgtgcctg ggatgccgtt accggttccg
    16261 ccgcgaacac gccaaatgac agagttgaaa gagccatagc agcaacaaca gtattgatag
    16321 ttttcataat taatctctcg caggtgattt ttgtataagt gacgttgttt cgtcgatgtg
    16381 atgagtatca cgttttttta cgagagataa aatcgaagag aattgaccgc cttgttcaaa
    16441 taaattgatt gatatctaac atactgataa accatttatt tgttgtactt atggacttgt
    16501 tttatgccgc gtggcagtca cagcagcgtt aaaggcacgg aatgccacgc aaaatacaaa
    16561 ttatactcgc tatgcatctc gccagttgta gttaacgtaa ctggctgtct ttcgaacctc
    16621 tgcgattata tcctgtatac gctggttttt gtaaatcttt ctcctgctga caatgaatac
    16681 ataagcgcac gccaggaatg gcttcccgac gggcttgcgg gatgggggca ccgcactctt
    16741 cacattcata caggctttcg ccacgcggaa tttcaccccg agcgcgggca atcgcatctt
    16801 caattgtact gttgatctgt tcgttgacgg cgtcatcgtt agcccaagcg gatgccatat
    16861 cgtcctcccc atatcaatgc ttgtacagtt aagtgtagct aatataggga cgaacctggg
    16921 cagttcaagc gtcagatctc cgaccattcc cgcagcagat tatgataaag attgagcagc
    16981 gacaggatct cttcattttc accatagcgg cttttcagcg actgaatatt tttgtccagt
    17041 tcaaacagca tggcgcgctt tttatcatcg cggatcatcg actggatcca cataaatgat
    17101 gccactcgta cgccgcgggt tacgggtgtc acgcaatgca ggctgctgga gggatacaac
    17161 agcagatcac ctgccgggag ttttacccga tgttgtccaa aggtgtcatt aacgaccagt
    17221 tcgccgccgt cgtagctttg tggatcgctt aaaaacagcg tggcagaaag gtcagtgcgc
    17281 atccagccgt tttgtggatg gctgcgtact gcgccatcca catggaaacc ataggtttca
    17341 ttgttctgat agcgattaaa cagcggcgtg gaaagggtac gcggcaaggc agcggcaaag
    17401 aacaatgcat gttggttaac cgcgttcagc acctcatttt gcagtgcggc gtataacgcg
    17461 ctgcgggtgt ctacttgctg attgttctta acttgtgcac cttgtgctcc ggtggtgacg
    17521 cgtccatcca cccattcggc ttgttccagt tgttcgcgaa aatgggcgac gtcctgtggc
    17581 gataacacgc cgggaatgtg gtacatcata caagttctcc aaaaagtggg gcctgcgccc
    17641 cacatctgaa tcagaaatgc atattggctg tgagcaagaa ggttcttggc tcgcccgggt
    17701 gataacggta gccgctcttg ttgattgagg cgacgtaatc ggtatcaaac aggttgtaaa
    17761 cgtttagctg gaagtcgaga ttgcgattaa cccgataccc cagtttggca tctgcgaccc
    17821 agtaaccttc tgtaaatgct ggcgttccca ccgcgccgtc tgaaccttta tgtatactgc
    17881 cgatatagcg tgcgcccgcg ccaacagaga tatcatcggt tgcctgatat tggctccata
    17941 aggtgaaggc gtgctccggg gtatacggca gcgatgagga accatcctgg gcaacatctt
    18001 tgccgttttt gatggttgct tttttctggg tatagccgcc aatcacctgc cacgcgggag
    18061 tgatattccc tgccacggat atctcatagc cttcgacgcg tttcttaccg tattgcgagt
    18121 aagtcccgtc atcattttgc tcaacttcat tttcgatatc agtacggaac agcgcggcgg
    18181 tgagcaacag gcgtttatcc agaacctgcc atttggtgcc aatctcgctg gtgttggctt
    18241 tttgcggttt aaaatcggtg cggttggcac tgttaccact gccagactgc gcaagggcga
    18301 agttgttgcc gcccggaggc tgctgggaaa cggcatagtt aatatagaca ttgccgtttt
    18361 ccgtcaggtg atacagcgcc ccggctttcc agttcaccag attgcccgac ttggcggtgt
    18421 cgacggtggt gactggagaa ccttttgcca caccagctgg gcaggtgatg gcaccgcgtc
    18481 cgctgccgcc gcaggcggtg gcactgtcat attcagtatg ataattatcc agacggatcc
    18541 cgccgttcag ctcaaaatca cgggtgattt gcagcgtatc gaaggcgtaa attgcgaagg
    18601 tatccgtctg accattggcg tttgcgccgt tgcgcgtcag gccgccggga tgaattctgc
    18661 tgtcaggatg ataaatattt accgccggta acgtcaccgg attaacgcca tagttagtct
    18721 gcgtctcacg ggtaaattcc acgccggtac tgacatcatg accgatagaa ccggtataga
    18781 acgtcgaggt caggttggtc tggttggtga gaattttatt actcacatct ttggtattcg
    18841 ccgtgcgtga ctaggtccag ctattcacat cgctggtggg ctgagtaata ttcgacgccc
    18901 cgcccataat cgccgtcatc aggtaatcct gctttacgcg cgaccaacgg gtagtattgc
    18961 gaatggtggt gttatcgttg atgtcgtgct caaaacgcat ggtggcggtg tcggtggtcg
    19021 aatcgtcgta atcggaatcc gtgccgtaaa agttatgagt atcaactttt ccggaatgat
    19081 tcagggccgc cgctcccgca gatggggcag aatagcccgg caaaccgatg gtcggaatgc
    19141 cgccgtctgg cgtgttgtgc tgagtgacat gcagataatt aagatacaaa cgattcgctg
    19201 taccaaggcc aaaagagaca gaaggggcga cgccgtaacg ctcatttttg actttgtcgc
    19261 gtccggcatc gtgggttttt tcgcccatca cattcaggcg caccgcggta gtatcaccaa
    19321 tgacctgatt gacgtccagc gtgccgcggc ggaaccaggc gctgccaata ctggcggagg
    19381 cgtcaatgcc ggaatcattg cgcggctgct tgctgatcat attgatcgag cctgtcggtg
    19441 cgctgcgccc gtagtcggtg ccggacggcc ctttaatcac ttcgacctgc tcggtattga
    19501 aggtgtcgcg cgagacgctg ccgatatcgc gaataccatc aatataaata ctgttagagg
    19561 tatcggcacc acgcatataa atggcgtcgc cagtggtgga gttaccgttc tcacccgcaa
    19621 aaaacgca
//
LOCUS       DAJTNM010000072        19515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTNM010000072 DAJTNM010000000
VERSION     DAJTNM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.072"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..170
                     /locus_tag="OO172_002588"
     CDS             <1..170
                     /locus_tag="OO172_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5512729.1"
                     /translation="NRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFG
                     MTPGEYRRKFR"
     gene            complement(342..671)
                     /locus_tag="OO172_002589"
     CDS             complement(342..671)
                     /locus_tag="OO172_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5512730.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(676..861)
                     /locus_tag="OO172_002590"
     CDS             complement(676..861)
                     /locus_tag="OO172_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5512731.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(858..3497)
                     /locus_tag="OO172_002591"
     CDS             complement(858..3497)
                     /locus_tag="OO172_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5512732.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3705..4694
                     /gene="ldhA"
                     /locus_tag="OO172_002592"
     CDS             3705..4694
                     /gene="ldhA"
                     /locus_tag="OO172_002592"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5512733.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4805..5152
                     /gene="hslJ"
                     /locus_tag="OO172_002593"
     CDS             4805..5152
                     /gene="hslJ"
                     /locus_tag="OO172_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5512734.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEISFGEKMMISGSMCNR
                     FSGEGKLSNGELTAKGLAMTRMMCANPQLNELDNTISEMLKEGAQVDLTANQLTLATA
                     KQTLTYKLADLMN"
     gene            complement(5149..5415)
                     /locus_tag="OO172_002594"
     CDS             complement(5149..5415)
                     /locus_tag="OO172_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5512735.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5689..9213
                     /gene="nifJ"
                     /locus_tag="OO172_002595"
     CDS             5689..9213
                     /gene="nifJ"
                     /locus_tag="OO172_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5512736.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9580..10709
                     /gene="ompN"
                     /locus_tag="OO172_002596"
                     /pseudo
     CDS             9580..10709
                     /gene="ompN"
                     /locus_tag="OO172_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            10850..11284
                     /gene="uspF"
                     /locus_tag="OO172_002597"
     CDS             10850..11284
                     /gene="uspF"
                     /locus_tag="OO172_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5512737.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11460..12395
                     /gene="ttcA"
                     /locus_tag="OO172_002598"
     CDS             11460..12395
                     /gene="ttcA"
                     /locus_tag="OO172_002598"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5512738.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12524..13891)
                     /gene="dbpA"
                     /locus_tag="OO172_002599"
     CDS             complement(12524..13891)
                     /gene="dbpA"
                     /locus_tag="OO172_002599"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5512739.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTLALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14369..15352)
                     /gene="zntB"
                     /locus_tag="OO172_002600"
     CDS             complement(14369..15352)
                     /gene="zntB"
                     /locus_tag="OO172_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5512740.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15607..16839
                     /gene="dgcM"
                     /locus_tag="OO172_002601"
                     /pseudo
     CDS             15607..16839
                     /gene="dgcM"
                     /locus_tag="OO172_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(16860..17423)
                     /gene="smrA"
                     /locus_tag="OO172_002602"
     CDS             complement(16860..17423)
                     /gene="smrA"
                     /locus_tag="OO172_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR5512741.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17677..19131)
                     /locus_tag="OO172_002603"
     CDS             complement(17677..19131)
                     /locus_tag="OO172_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5512742.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19128..>19515)
                     /locus_tag="OO172_002604"
     CDS             complement(19128..>19515)
                     /locus_tag="OO172_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5512743.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5129 a         4956 c         4591 g         4839 t
ORIGIN      
        1 gtaaccgtcg tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac
       61 tggcaggcat cggctttcat tggggatttt ctgaccagag tcatttctca acggtattta
      121 agcaacgctt tgggatgacg ccaggcgagt atcgccgtaa attccgctaa ctaattggcg
      181 ttgcatgtac tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc
      241 gaaaggcaat ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc
      301 gctttctcct gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat
      361 ttaccgttta tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg
      421 gcgagtttcg cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc
      481 gctttacgtt cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc
      541 gcaaccagat aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga
      601 gtcaatgcca ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt
      661 ttcttcatca tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt
      721 gtctgccttg atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc
      781 cttaggtgca gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa
      841 cgcagccagt aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt
      901 cccggcagac gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg
      961 cgccacagcg taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta
     1021 ccatcaacgc gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct
     1081 aagttaattt ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa
     1141 cccgcagtca tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc
     1201 attggcccgc tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat
     1261 aacggcaatg caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta
     1321 accagttcgc tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata
     1381 cgtaattgtt tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc
     1441 aagggttctt cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct
     1501 gtcaccagat taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc
     1561 caggctccat cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct
     1621 ggcatccagg cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca
     1681 ggcgcagcag aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag
     1741 tcgggtggca ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg
     1801 cgcagacgaa taccgtccca gcgaccattt acccgaaccg ggccaatctc accaacatgt
     1861 aaatcgcctt taaagaggaa ataggtcgga tcgcgcccat cgacgctaaa ttttaaggtt
     1921 gatggcggta acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg
     1981 ccgctgaagg agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat
     2041 cgcggctttt ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg
     2101 gtcagcgtaa tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc
     2161 atcggtgtaa aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga
     2221 aaatcattcg ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc
     2281 tgcaaaatgg cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa
     2341 ggccagcgga tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac
     2401 gcgcctggct caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa
     2461 cgggcatata aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga
     2521 ctgttatcca tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct
     2581 tgcccctggg tcagcacact cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc
     2641 cagttgtcaa ctttgacacc cagtcggcca ctcaaaggaa aacctgcata cagccagctc
     2701 cagcgaccat cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac
     2761 ggatcgccgt tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc
     2821 tcggcatcca ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt
     2881 acaggaagtc catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc
     2941 tgattttcaa acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg
     3001 ccttgaaatt taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg
     3061 agagagagtt ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc
     3121 caggtgttag gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc
     3181 tccgtctgcg gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat
     3241 aaccagcggc tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc
     3301 accagataac ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct
     3361 aatgcaatac gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg
     3421 agcgtcatca gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct
     3481 ttatatttac ccagcatggg tagttaatat cctgatttcg cgaaaaatta agcattcaat
     3541 acgggtattg tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt
     3601 gttgatgaat ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat
     3661 ttttagcagc ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt
     3721 atagcacaaa acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg
     3781 agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg
     3841 aagcggtatg tattttcgta aacgatgacg gcagccgccc ggtgctggaa gagctgaaaa
     3901 agcacggcgt taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg
     3961 cggcaaaaga actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg
     4021 ctgaacacgc catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc
     4081 gtacccgtga cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa
     4141 cggcaggcgt tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt
     4201 ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg
     4261 tggagtatgt cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc
     4321 cgctgacacc ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg
     4381 gcgtgatgat cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag
     4441 cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc
     4501 tgttctttga agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg
     4561 cctgccacaa cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca
     4621 gtatttctca gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga
     4681 acgaactggt ttaatcttgc cgctcccctg caatccaggg gagctgattc agataatccc
     4741 caatgacctt tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac
     4801 cacaatgaag aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag
     4861 taatgacaaa attgctgtta cgccagaaat cagctttggt gaaaaaatga tgatttccgg
     4921 cagcatgtgt aaccgcttta gcggtgaagg caaactgtct aatggtgaac tgacagccaa
     4981 agggctggca atgacccgta tgatgtgcgc taacccgcag cttaatgaac tcgataacac
     5041 cattagcgaa atgctgaaag aaggtgcaca agtggatctg accgcgaacc agttaacgct
     5101 ggcgaccgcg aaacagacat taacttataa gctggcggat ttaatgaatt aatagctgcc
     5161 acagctcccg gcggcaagtg actgttcgct acagcgtttg ccgttgggta atgcacacat
     5221 cccaatcgcc gtaccatcca gttgacgggc aacagaaagc gaaccgccga tcattgcaca
     5281 atttgcttct ccactactgg acatcgacgc ttttaaactt ggcgctacgt gcgccgcagt
     5341 cgcctgctga acaggctcac tactacacgc cgacaacaat aaagcggcac accctaccca
     5401 aaacgctgct cgcatctctt cttcctctga tcttcaagcc aaacgacacc gccataaata
     5461 ataggcagca cagagggcgg cgtcgagagc tgtcctgcgc gttgccccgc atttttactt
     5521 ttttatggct atttttttgc cctctgtttg atcaaaacat tcattactct gatgtggggg
     5581 acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa ttgaaggatt actactgggc
     5641 gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag ggtgtcatat gattactatt
     5701 gacggtaatg gcgcggttgc ttcggtggcg tttcgcacca gtgaagttat cgccatctac
     5761 cctattaccc ccagttccac gatggcagaa caggctgatg cctgggccgg aaacggcttg
     5821 aagaacgttt ggggagacac accacgcgtg gttgaaatgc agtcggaagc gggtgctatc
     5881 gctaccgtgc atggcgcttt gcagacgggt gccctttcaa catcgtttac gtcatcgcag
     5941 ggtttgctgc tgatgatccc gacgctgtac aaactggcag gcgaactgac gccatttgtc
     6001 ctgcatgtag cggcacgtac cgttgccaca catgcacttt ctatttttgg cgatcattcc
     6061 gacgttatgg cggtgcgcca gacgggttgc gcgatgttgt gtgcggctaa cgtccaggaa
     6121 gcgcaagact ttgctctcat ttcacacatc gcaacgctga agagccgcgt gccatttatt
     6181 catttctttg atggttttcg cacgtcccac gaaatcaata aaattgtccc gctggccgat
     6241 gacactattc ttgatctcat gccgcaggcc gaaattgatg ctcatcgcgc ccgggcgctc
     6301 aacccggaac atccggtgat ccgcggtacg tccgccaatc ctgacactta tttccagtct
     6361 cgcgaagcca ccaacccctg gtacaacgcg gtctatgacc atgttgaaca ggcgatgaat
     6421 gatttcgctg ccgcgacagg tcgtcagtat cagccgtttg agtattacgg gcatccgcaa
     6481 gcggaacggg tgattatcct gatgggctct gccattggca cctgtgaaga agtggttgat
     6541 gaattgctaa cccgtggcga aaaagttggc gtgctgaaag ttcgtctgta ccgtcccttc
     6601 tccgccaaac acttgctgca agctctgccg ggatccgtac gcagcgtggc ggtactggac
     6661 agaaccaaag aacccggtgc ccaggcagaa ccgctctatc tggatgtgat gaccgcactg
     6721 gcaggagcct ttaataatgg tgagcgcgaa accctgcccc gtgtcattgg tgggcgctat
     6781 ggtctttcat ccaaagaatt tggcccggac tgtgtactgg cggtatttgc cgagctcaac
     6841 gcggctaaac ctaaagcacg ctttacggtt ggtatttacg atgatgtgac caatctgtca
     6901 ctgccgttgc cggaaaacac tctgccaaac tcggcgaaac ttgaagcctt gttttatggc
     6961 cttggtagtg atggcagcgt ttccgcaacc aaaaacaata tcaagattat cggtaattcc
     7021 acgccgtggt acgcacaggg ctattttgtt tacgactcca aaaaagcggg cggcctgacg
     7081 gtttctcacc ttcgcgtgag cgaacagccg attcgttccg cttatctcat ttcccaggct
     7141 gattttgttg gctgccacca gttgcagttt atcgataaat atcagatggc tgagcgttta
     7201 aaacctagcg gcattttcct gcttaatacg ccgtacagcg cagatgaagt gtggtcgcgc
     7261 ttgccgcagg aagttcaggc cgtgttaaac cagaaaaaag cgcgcttcta tgtgattaac
     7321 gcggcgaaaa tcgcccgcga atgtggcctg gcggcccgta ttaataccgt catgcagatg
     7381 gcttttttcc atctgacgca aattctgcct ggcgatagcg ccctcgcaga attgcagggt
     7441 gcgattgcca aaagttacag tagcaaaggt caggatctgg tggagcgcaa ctggcaggct
     7501 ctggcgctgg cgcgtgaatc cgtagaagaa gttccgttgc aaccggtaaa tccgcacagc
     7561 gccaatcgac cgccagtggt ttccgatgcc gcccctgatt tcgtgaaaac cgtaaccgcg
     7621 gcgatgctcg ccgggcttgg tgatgccctc cccgtttcgg cgctgccgcc agacggcacc
     7681 tggccgatgg gcaccactcg ctgggaaaaa cgcaatatcg ccgaagagat ccccatctgg
     7741 aaagaggaac tctgtaccca atgtaaccac tgcgtcgccg cttgcccaca ctcagctatt
     7801 cgcgcaaaag tggtaccgcc tgaagcgatg gaaaacgccc ctgccagcct gcattcgcta
     7861 gatgtgaaat cgcgtgatat gcgcgggcag aaatatgtct tgcaggtggc accggaagat
     7921 tgcaccggtt gtaacctgtg cgtcgaagtt tgcccggcga aagaccgtca gaatccagag
     7981 attaaagcca tcaatatgat gtctcgccag gaacatgtcg aagaagagaa aatcaattac
     8041 gatttcttcc tcaacctgcc agaaatcgac cgtagcaaac tggaacgtat tgatattcgt
     8101 acatcgcagc tgattacacc gctgtttgaa tattcaggtg cttgccccgg ttgtggcgag
     8161 acgccgtata ttaaattgct gactcagctc tatggtgacc ggatgttgat cgctaacgcc
     8221 accggctgtt cttcaattta tggcggtaac ctgccctcta caccgtatac caccgatgcc
     8281 aacggtcgtg ggccggcatg ggcgaactct ctatttgaag ataatgccga atttggcctt
     8341 ggtttccgcc tgacggtcga tcaacaccgt gtccgcgtgc tgcgtctgct ggatcaattt
     8401 gccgataaaa tcccggcgga attactgacg gcgttgaaat cagacgctac gccagaggtt
     8461 cgtcgagaac aggtcgcagc tttacgccag caactcaacg atgttgccga agcacatgaa
     8521 ctgctgcgtg acgcagatgc actggtggaa aaatcaatct ggctgattgg tggcgacggc
     8581 tgggcttacg atatcggctt tggcggtctg gatcatgtat tgagtttgac ggaaaacgtc
     8641 aacattctgg tgctggatac gcaatgctat tccaacaccg gtggtcaggc gtcgaaagcg
     8701 acaccgctgg gtgcagtaac taagttcggc gagcacggca aacgtaaagc gcgtaaagat
     8761 cttggcgtca gtatgatgat gtacggtcat gtttatgtgg cgcagatttc tctcggcgcg
     8821 cagctgaacc agacggtgaa agcgattcag gaagcggaag cgtatccggg accatcgctg
     8881 atcattgctt atagcccgtg tgaagagcat ggttacgatc tggctctcag ccacgaccag
     8941 atgcgccaac tcactgctac cggcttctgg ccgctatatc gctttgatcc gcgtcgtgcc
     9001 gatgaaggca aactgccgct ggcgctggat tcacgcccgc cgtcagaagc actggaagaa
     9061 acgttacttc acgagcagcg tttccgtcgt ctgaattcgc agcagccaga agtggcagaa
     9121 cagttatgga aagatgctgc agctgatttg caaaaacgct atgacttcct agcacaaatg
     9181 gccggaaaag cggaaaaaag caacaccgat taaatgctct ggataaggat tatccaattc
     9241 taaaaaaaaa gcccggacga ctgttcgggc ttgtcttttt atatgttgga aaatcagtgg
     9301 caatgcaatg gcccaacaga aaataaaatc tgtgacaata aaggcatata acccgcgcag
     9361 aataacgtat agaaaattaa aagtataatt tttattttgt atgtattatc ttcgatacat
     9421 tcaccagact tattatcatt tcagaggaat tatttgatta aggttttact taaggcgtaa
     9481 caaatgatat ttatcggcaa actgaacttc tcctttagga tgttttcact ccccgaaatg
     9541 gggataaaag gcaaataaaa taactaaagg atttattcaa tgaaaagaaa agtactggca
     9601 cttttaatcc ctgccctgct cgccgcaggt gctgcacatg cagccgaagt ttataataaa
     9661 gacggcaaca aattagatct gtatggcaaa gttgatggcc tgcattattt ttctgataat
     9721 tcagcgaaag atggcgacca gagctatgcg cgtctgggtt ttaaaggcga aacccaaatt
     9781 aacgatcaac ttactggcta cggtcaatgg gaatacaata ttcaggcaaa caacactgaa
     9841 tcttcaaaaa accagtcatg gacccgtctg gcatttgccg gactgaaatt tgcagattac
     9901 ggttctttcg attacggacg taattatggc gtaatgtacg acatcgaagg ctggaccgat
     9961 atgctgccta aatttggcgg tgactcttat accaatgcag acaactttat gactggtcga
    10021 gccaatggcg tcgcgactta tcgtaatact gatttcttcg gtctggtaaa tggtctgaac
    10081 ttcgcggtgc agtatcaagg taacaacgaa ggagccagta atggtcagga aggcaccaat
    10141 aacgaacgcg atgttcgtca tgaaaacggt gacggctggg gtctttccac aacatatgat
    10201 ttaggcatgg gctttagcgc tggtgcggca tacacctctt ctgaccgcac caatgaccag
    10261 gttaaccata ctgcggcggg tggtgataaa gcagacgcgt ggactgctgg gctaaaatac
    10321 gatgctaaca atatttacct agcaaccatg tattcagaaa cgcgtaatat gaccccgttt
    10381 ggcgacagcg attatgcagt tgcgaacaaa acccagaact ttgaagttac tagtaccagt
    10441 ttgattttgg tctgcgtccg gcagtctctt tcctgatgtc taaaggccgt gaccttcacg
    10501 ctgctggtgg tgcagacaac ccggcaggtg ttgatgataa agatctggtt aaatacgccg
    10561 atgttggcgc gacttactat ttcaataaaa acatgtccac ctatgttgac tataaaatca
    10621 acctgttgga tgaagatgac agtttctacg ctgccaacgg catctctacc gatgatattg
    10681 tcgctttagg tctggtttat cagttctaaa tcctcctgcc cgctgttatg gcgggctttt
    10741 tctgcttatt cttcctcctt tgatctaaat taaaaatgtg aactccgtca ttacacaaaa
    10801 agtgtcatct ggcgttacac tttatgcgga tactaaaaca ggaggtttta tgaacagaac
    10861 gattcttgtc cctatcgata tttcagattc agaattaact caacgcgtga ttagccacgt
    10921 tgaggcagag gcaaagattg atgatgcaga ggttcatttc ctgacggtaa taccttcact
    10981 gccctactat gcctctctgg gtttagcgta ttccgcagaa ttaccggcaa tggatgacct
    11041 gaaagcggaa gccaaatcgc aactggaaga gatcattaaa aaatttaaac tgccaaccga
    11101 cagagtgcat gtccatgttg aggaaggctc gcccaaagac cgcattctgg aattggcgaa
    11161 gaagatcccc gctcatatga tcatcattgc ttcccatcga ccggatatca ccacttatct
    11221 gctcggttcc aacgccgcag ctgtagtgcg tcacgcagag tgctccgtgc tggttgtgcg
    11281 ctgacactaa cgcccgcaca ttgctgcggg ctttttgatt catttcgcaa atgtgctgac
    11341 attttcccct ctaatccgta ccatacacgc cacagttttt atatcagatt tcttatgctg
    11401 agcgttccgg catgacgcat actcttctga tgccatataa cgaattgagt cgcttttaaa
    11461 tgtcgcaaaa tcaagaaatt agtaagaaag aacaatacaa cctgaacaaa ttacaaaaac
    11521 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
    11581 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
    11641 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
    11701 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
    11761 agattgttga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
    11821 cttgctcact gtgttctcgg ctacgtcgcg gtatccttta tcgcaccgca acggaactgg
    11881 ggacgacgaa gatcgcgttg ggtcaccatc gtgacgatat cctgcaaacg ttgttcttaa
    11941 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
    12001 aacatatcgt tattcgtcca ctggcctact gccgcgagaa agatattcag cgatttgccg
    12061 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
    12121 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
    12181 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
    12241 atttcaaagg cattacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
    12301 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
    12361 atgagttacg gctgaatgtg gttgaagtga aataaccagg atagcgcccg atgcgcaagc
    12421 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
    12481 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
    12541 cccggcacgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
    12601 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
    12661 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
    12721 cttttttccc gccatcgata cacaacgttg ccatttctgc ttccagcgtc acaatggaac
    12781 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
    12841 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
    12901 ctgtacgacc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
    12961 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
    13021 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg ttgctccaga tcgccgtgta
    13081 acgacaatgc actttgctct acttcattca gcgcgtcgca gacagcctgg caatcttttt
    13141 tggtattgca aaacaccaca caagaggatg gctgatgcaa gcttaataac cgttgcaaca
    13201 gaggaatttt gcctttactg gatgtctcat aaaattgttg ttcaatgggt ggcaaagcat
    13261 ctgttgagtc aatttcaatc gccaaaggat cgcgttgcac tcgtccgctg attgcggcga
    13321 tggcttccgg ccaggttgcc gaaaacagaa gcgtctgtcg agatgcaggc gcaaaacgga
    13381 tgacatcatc aatggcatcg ctaaatccca tatccagcat gcggtcggcc tcatccatca
    13441 ccaaagtatt cagcgcatcc agtgataccg tgcctttttg caagtgatcc agcaaacgac
    13501 ccggcgttgc cacgataata tgcggcgcat gttgcaacga atcacgctgc ataccgaacg
    13561 gttgaccacc gcacaacgtc aaaattttgg tatttggcag aaaacgcgcc agccgacgca
    13621 attcacctgc cacctgatcc gccagttcac gcgtaggaca cagcactaaa gccagggttt
    13681 gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc aaaagccgcc gttttgccgc
    13741 tgccggtttt cgcctgcacg cgaacatctt ttcctgcaag gatcgccgga agcgcggcgg
    13801 cctgcaccgg cgtcatggtt aaataaccca actcattaag gttcgtgagt tgggcgggag
    13861 gcaaaacatt cagggtagaa aaagcggtca caatctattc tcgtggtcat cgacgcaaag
    13921 ttagcaggcg cgtatcctcg cagatctacg ctcacgatgc gacaatttaa tcggttcttc
    13981 atcgggtggt gggtcaggca tgggttgcgg gcgagggatc ggatcgggca ctggaacagg
    14041 atcgccagga atcggttcag ggacaggaat ttgcaaataa ataagtgtcg tcatatttcc
    14101 ctccggtcat tgggtggact cttaaagggt agacgctgat aaataacagg caaaaaaaag
    14161 ccgactcatc aaagtcggcg tcgtacgaat caattgtgct atgcagtaat tcaaaaaagg
    14221 aagtaagaca atatggagcg caacgcccat cgcttgacgt tgcattcacc tgcaagggag
    14281 atattgccct gaatgggtag agagtttatt gacttcgctc aaactttgcg gcgtttttgt
    14341 atacagacag ccgaaaaaat tgcttttgtt acaaccattt actacggtgc aaccataaag
    14401 caacaccacc aataagaaca actaacagaa tacaaaaaat tgaaaatccg aattgccacc
    14461 cgccgccagg gatcccacca aggttgacgc caaataaccc ggtcagaaag gtactgggta
    14521 aaaagaccat tgccatcaac gacattgtat aggtacgacg agctaaattt tcctgcatca
    14581 cctgagcgat ttcatccgcc atcacgccag tccgtgctat acaggcgtcg atttcgtcaa
    14641 ggccgcgccc aaggcgatcg gcaatatcct gcatccgacg gcgttggtca tcgctcatcc
    14701 acggcaaacg ttcactggca agacgagcat aaacatcacg ttgcggtgcc atatagcgac
    14761 gcatcacaat taattgtttg cgcagcagag ccaggaatcc acgcggtgga atttgctgat
    14821 caaggagatt atcttcaagg tcgataattt tatcgtgcag ctgctcgata aattcactgg
    14881 aatgatcggt caacgcatcg cacacatcca ccagccatcc cccgcaatcg gtcggacccg
    14941 tgccctcttc cagatcgctc accacatcgt ccagcgccag cactttgcgt tgtcgggtcg
    15001 aaacaattaa ccgcccgtcc atatatacac gcatggcgac cagttgatcg gggcgttcat
    15061 cggtgctgcc gtttatacag cgcaatgtaa tcagcgtgcc ttcaccgaaa cggctgactc
    15121 ggggccgtgt gctctcgccc gccagcgcat cacgtacgtt attgggaagc agcggtgttg
    15181 tcgccagcca ttgggcgcta tcatggtgta cataattaag gtggagccag cagggatgcg
    15241 cttcatcaat cacatctgta ttttccagcg gtttaacgcc gcctctacca tccagcatcc
    15301 aggcaaatac tgcatccggg acattaacgt ccgatccctt aatcgcttcc acagtgcctc
    15361 catcatcaac gcattatttt gtagtctagc cttctggccc tgttacgcaa catctcatca
    15421 ccccattacc ctgaaatgat taataaaatt ctgtctaaat tgaatacaaa aagcaaaatg
    15481 cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc agaattatct
    15541 gatcatatga cgtggctttt ttgcgatcgg atagcaacaa aaattgataa aaataacggg
    15601 atctcaatga ttacgcacaa cttcaatacc ctggacttac tcaccagtcc tgtctggatt
    15661 gtttcgccct ttgaggaaca gttaatttat gccaatagcg cggcgcgact gttgatgcaa
    15721 gacctcacgt ttagtcagct acgaaccgga ccctattccg tctcctcaca aaaagaactg
    15781 ccgaaatacc tctccgatct gcaaaaccaa cacgatatta tcgaaatcct cactgttcag
    15841 cgtaaagaag aggaaacagc attaagctgt cggcttgttt tgcgagagct gacagaaaca
    15901 gaaccggtga ttattttcga aggtatcgaa gcgccggcaa cgctgggttt aaaagccagt
    15961 cgctcggcaa attatcagcg caaaaaacaa ggtttttatg cgcgcttttt tctgactaac
    16021 tctgcaccaa tgttgttgat tgacccgtca cgagatggac aaatcgtcga tgctaacctc
    16081 gccgcgctca atttctatgg ttataaccat gaaacgatgt gccagaaaca tacctaggaa
    16141 ataaatatgc ttgggcgtcg cgtcatgcct atcatgcatg aaatttcgca tttacccggc
    16201 ggtcataacc ctttgaattt tgttcataaa ctggcggatg gttctactcg tcatgtgcag
    16261 acctatgccg gaccgattga aatttatggc gacaagctca tgttatgtat tgtgcatgat
    16321 attactgagc aaaaacggct ggaggagcag ctggaacatg ctgctcacca tgacgcgatg
    16381 accggattac tgaatcggcg acagttttat cacattacgg aaccaggcca aatgcagcat
    16441 ctcgccatcg ctcaggatta cagcttgttg ctcatcgaca ccgatcgttt taaacacatt
    16501 aacgatctct atgggcattc taaaggtgat gaggtgttat gcgccctcgc ccgccccctc
    16561 gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg gaggcgaaga gtttgtctta
    16621 ttgctaccaa gaaccccact ggataccgcg ctttcgctgg ctgaaactat ccgcgtaagc
    16681 gtggcaaaag tgagtatttc gggcttacca cgctttaccg tcagcattgg tgtggcgcat
    16741 cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg ttgatgatgc tttgtatcgg
    16801 gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag ccgcggatgc gtctcgagat
    16861 caacgactgc gcttagcgtg gcgctcccag ttttcttgct tcgcctgcgc cgttttacgt
    16921 agtgcgacgt aacacgcccc gctgccgcca tgatgcggta gcgcggtgca atatgcctga
    16981 acatcatcaa attcggtcag ccagcgcgcc acatagctgc ggacaatatt ggcatgtgat
    17041 ttatcttccc gccctttacc atgaataatc agcacattac gcaaaccatc cgccagggct
    17101 tgttgaatga aactgaacac cattttgcgg cactcttcca ccggctggcg caaaagattc
    17161 aggctcgcct gttgcggata tttaccactg cgcagcttat ccagcacccc atgctgcaac
    17221 ccttcctgcc gaaactccag cggctgactt agcgggatga tgtcgagaaa tccggtagtg
    17281 aggaaattat caagctgcag cgtgtcgata cgctgcggcg cacgttggtt acgcgttgga
    17341 tgccagtgga catctgtagc acgtttcagc ggctggacat cttccatggc gtcaagaaac
    17401 agcgatttgt cgtcaaggtt catgtaacct cctatgcacc ttcacacgtc gtgattcaga
    17461 cgggttaatt gttcatactt actgcatcgt tcccttcaca gattgaaaca atactctact
    17521 tcatacctgt gagtatctgc gcaggcgggg cattttatac tagcctgtta gcagtatcga
    17581 acagtttagc ccgagtttaa ttcagcacag gctccgatca ggatgattta ttcatagcag
    17641 gaaccaacaa aaatgcgata aataacagac cactgattag cagaaaggta ccgaaaatgc
    17701 catagtttgc gcctccctca gcatcaattg actcaataat ttggttgaag ccagtatttc
    17761 cgatgtctgg tcacttctac tgcgattaaa aaataaagca cttctggaaa tgaagtcatt
    17821 caccgtatgg ggaacagaaa ccattttttc acccattctc gaaaaatgaa aattagttcg
    17881 attattgaga atagagccac acagggcaat accaatcgcg cccccaagat tacgtgttaa
    17941 attaaataca ccagaggcta acttcagccg ttcttttgga attcccccca gcgttaatgt
    18001 tacgatgggg gccatcgcaa actgctgcaa aatccctcta attgcctgtg gaaacagtaa
    18061 ttcctgccat ccccattcgt gggttatagg ggtaaaaaga tacattgaaa acacaaatcc
    18121 gcccagcccc gccatcaaca gccatcgcag atttattttt ttacttaacc agaagtaaaa
    18181 gggaacagaa aataactgga aaatacctgt tgtgcaaacg gcaaaaccta tctcttcggc
    18241 atttaatccg cgaatctgcc ccaaaaacac aggtattaaa tatacagtag agaatatccc
    18301 aacccctcca gaaaaagaaa aaaaacatcc cagagtgaaa tttttatcct taaaagcatg
    18361 aagatccatt atcggattgg atattgtaag tgtacgcgca gcaaataaaa taaaactcac
    18421 caatgccaga actgatgtca gcaaaatagt attatcatcc agccatcccc agcgcgctcc
    18481 ttcttcaagg gtatattcca gacatcccag agtcatggcc aacaggataa tactcggata
    18541 atcagcaact ttcagtaaag agagatcagg tttatcaaaa ttaaccagaa aaggaatact
    18601 caaaactaaa tatatgccag gtaatatatt gatataaaat agccatcgcc agtcgaggtt
    18661 atcagtgagc cagcccccca gagttggccc tagcgtcggt gacagtgagg ccagcgcact
    18721 gactaccgca gcagccaacc ccaactcttt accctggtaa taaataaacg ccgtcgtgaa
    18781 aactaaaggg atcatggatg cgccagcagc tccctgtaaa gcacgaaaca aaatcatgat
    18841 ctgaatattc catgcaagtc cacacgcgat actcattaaa gtaaatattc ccgcggatag
    18901 agtaaaaagc caacgagtgg aaaatacccg tgacagccac ccagagagcg gaataacaat
    18961 aatctcagca attaaatagc tggtctgaag ccaggccatt tcgtctttac ctgcggacag
    19021 accaccacct atttcattta gggatgacga tacaatttga atatcaagct gagcaataaa
    19081 taatcccata cacatactgg cgaaagcaat atgtttacgc atagacatca tctctcatca
    19141 acactgacgg tgacggacag tcccgggcga agaagttgct ttaattcctt tgcgtcgtca
    19201 aatgcaatcc gtaccgggac acgctgaaca attttggtga aattgccagt ggcattttct
    19261 atcggaatca agctgaaact ggctcctgtc gctggggaaa ggctttcaat gtgaccgtga
    19321 aacacttccc ctttgagaat atcagcacgg atctcagcct tcattcctgg cttcattccc
    19381 gctatttggt tttctttata atttgcatca acccacaact cactgactgg tactagtgaa
    19441 actaacgatg tgccgccttc cacccaactg cctgtatggg cgctgcggtt tgcgacaatg
    19501 ccatcaatcg gcgca
//
LOCUS       DAJTNM010000073        19482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTNM010000073 DAJTNM010000000
VERSION     DAJTNM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.073"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..312)
                     /locus_tag="OO172_002605"
     CDS             complement(<1..312)
                     /locus_tag="OO172_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5512744.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NS"
     gene            697..885
                     /locus_tag="OO172_002606"
     CDS             697..885
                     /locus_tag="OO172_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5512745.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            836..1531
                     /locus_tag="OO172_002607"
     CDS             836..1531
                     /locus_tag="OO172_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCR5512746.1"
                     /translation="MGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETISCWLARTII
                     QTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFKVQPYPK"
     gene            complement(1643..1990)
                     /locus_tag="OO172_002608"
                     /pseudo
     CDS             complement(1643..1990)
                     /locus_tag="OO172_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(1959..3616)
                     /gene="dpiB"
                     /locus_tag="OO172_002609"
                     /pseudo
     CDS             complement(1959..3616)
                     /gene="dpiB"
                     /locus_tag="OO172_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            3997..5055
                     /gene="citC"
                     /locus_tag="OO172_002610"
     CDS             3997..5055
                     /gene="citC"
                     /locus_tag="OO172_002610"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5512747.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5070..5366
                     /gene="citD"
                     /locus_tag="OO172_002611"
     CDS             5070..5366
                     /gene="citD"
                     /locus_tag="OO172_002611"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5512748.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5363..6271
                     /gene="citE"
                     /locus_tag="OO172_002612"
     CDS             5363..6271
                     /gene="citE"
                     /locus_tag="OO172_002612"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5512749.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACVREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6282..7814
                     /gene="citF"
                     /locus_tag="OO172_002613"
     CDS             6282..7814
                     /gene="citF"
                     /locus_tag="OO172_002613"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5512750.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            7818..8369
                     /gene="citX"
                     /locus_tag="OO172_002614"
     CDS             7818..8369
                     /gene="citX"
                     /locus_tag="OO172_002614"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR5512751.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8344..9222
                     /gene="citG"
                     /locus_tag="OO172_002615"
     CDS             8344..9222
                     /gene="citG"
                     /locus_tag="OO172_002615"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5512752.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9273..10736
                     /gene="citT"
                     /locus_tag="OO172_002616"
     CDS             9273..10736
                     /gene="citT"
                     /locus_tag="OO172_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR5512753.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            10850..11656
                     /gene="rna"
                     /locus_tag="OO172_002617"
     CDS             10850..11656
                     /gene="rna"
                     /locus_tag="OO172_002617"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5512754.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            11885..12295
                     /gene="rnk"
                     /locus_tag="OO172_002618"
     CDS             11885..12295
                     /gene="rnk"
                     /locus_tag="OO172_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5512755.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12526..13763)
                     /locus_tag="OO172_002619"
                     /pseudo
     CDS             complement(12526..13763)
                     /locus_tag="OO172_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            13984..14412
                     /gene="uspG"
                     /locus_tag="OO172_002620"
     CDS             13984..14412
                     /gene="uspG"
                     /locus_tag="OO172_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5512756.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14533..16098)
                     /gene="ahpF"
                     /locus_tag="OO172_002621"
     CDS             complement(14533..16098)
                     /gene="ahpF"
                     /locus_tag="OO172_002621"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5512757.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16343..16906)
                     /gene="ahpC"
                     /locus_tag="OO172_002622"
     CDS             complement(16343..16906)
                     /gene="ahpC"
                     /locus_tag="OO172_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5512758.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17278..18024
                     /gene="dsbG"
                     /locus_tag="OO172_002623"
     CDS             17278..18024
                     /gene="dsbG"
                     /locus_tag="OO172_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5512759.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKHGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18234..19136
                     /locus_tag="OO172_002624"
     CDS             18234..19136
                     /locus_tag="OO172_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5512760.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19283..19482
                     /locus_tag="OO172_002625"
                     /pseudo
     CDS             19283..19482
                     /locus_tag="OO172_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         4900 a         5011 c         4670 g         4901 t
ORIGIN      
        1 tgaattaaat attctcagtg cattgaccgt cgatattttt acttcactgc tgaaaagggc
       61 tttaacaaga acatacagag catcccattt aagattcatc aggtctttaa cccggccgcc
      121 agtgaggtgg tgtctccatt tgaaatactc attgccgttg tcgcctgttt caaaccacat
      181 gtatgaatca gtatcgctgt cggcatcatt tttaaatcca atctttgccc agtcagtatt
      241 ccgaatccag gcaaggattg agtcgttttc aaaagtaagt ccaccggaca acgtatcgcc
      301 atttttttgc acggcgttac cagccttgtt taccgtttcc tgtaaaccga ggggccgttt
      361 ctggcctgca tggcatgatt tccgctttta aacggggaat tcagcgtgct gattggctat
      421 gtaagggtat caacaaatga ccagaataca gacctgcaac gaaacgctct tgtttgtgca
      481 ggatgtgaat aaatatttga agataaatta agcggaacaa ggacagaccg accgggatta
      541 aaacgtgctt taaagcgcct tcaaaaagga acgcatatag aaaacgacga tcgaatcaat
      601 taaatcgatc tgtattaccg atcgattatg tcccgataac cacacccgac tgatgagatt
      661 ttttaagata acggcgaatt gatgcaagga ggtgaaatga aatcaattcg ctgtaagaac
      721 tgcaacaaac tgttatttaa agcggattcc tttgatcaca ttgaaatcag gtgtccgcgt
      781 tgcaaacgtc acatcataat gctgaatgcc tgcgagcatc ccacggagaa acattgtggg
      841 aaaagagaag aaatcacgca ttctgacgaa accgtgcgtt attgagtatg aaggccagat
      901 tgttggctat ggttcaaagg agctgcgcgt tgaaaccata tcctgctggc tggcccgcac
      961 aatcattcag acaaagcact attcccgccg ttttgtgaat aactcttacc ttcacctggg
     1021 agtattcagc gggcgtgatc tggttggcgt tctccagtgg ggatatgccc ttaaccccaa
     1081 ctcaggtcgt cgtgtcgtgc ttgaaacgga taaccggggc tatatggagt tgaatcgcat
     1141 gtggctacac gacgacatgc cccgcaactc tgaatcacgg gccatcagct acgcgctgaa
     1201 agttatcaga ttactgtatc cgtcagtgga gtgggttcag tcctttgctg acgaacgctg
     1261 cggactcgca ggcgttgtgt atcaggcgtc gaattttgat tttattggca gccatgaaag
     1321 cacgttctac gagctggatg gtgagtggta tcacgagata acgatgaacg cgattaagcg
     1381 cggagggcaa cgaggcgagt atttgcgagc taataaggag cgtgccgtgg tgcacaaatt
     1441 taatcagtat cgctacatca gattcctgaa caagcgagca aggaagcggc taaataccaa
     1501 actattcaag gttcagccat accctaagtg atccccatgt aatgaataaa aagcggtaat
     1561 taatactgct gtttcatttg aagcgcgaaa gctaaagttt tcgcatttat cgtgaaacgc
     1621 tttcgcgttt ttcgtgcgcc gcttcaccca gccgttcata ggcaattggc ttaatgagat
     1681 aatcaaatac accacaacgt acggcttcag acaccgtttc catatcactg gctgcagtgg
     1741 taaacaccac gtcgccggga taatgcgcct gcaccagttc atgcagtaaa ttaatccctc
     1801 taccgtcagg aagatagtta tcgagcaaga ttagccccgg cttaaaacgc tcgatcatca
     1861 ttcgggcctg cgccaggttt cccgccagta atatctgact gaatccggga atgtgacgaa
     1921 tatattccgc atgcatctct gccagcggcg tttcgtcctc aacgatcaat agggttaatg
     1981 gagctgtcat taggtttcac tttcggaata tagattgaaa ataaggtacc gcagggatca
     2041 ttatcttcga gagtgataac accaccgcag cgcgttacgt agctggcaat caagtacaac
     2101 ccaatgccat gttcaccggg ctcgtcagca cgcgtactga cccctgctca aatattttgt
     2161 ctcgtagaga ctctggaacg ccgcagccct gatcggcgac ttcaatcacc acatcatcgc
     2221 cttcatcgct gaggaataat tcaacgctct tgtttccttc atcgctacgc aggctggctt
     2281 cgaaggcgtt atcaagtaaa ttgccaacaa tggcggcaaa ctcggtgcta tccagtcctg
     2341 gcggcagttg cgaaagctgg ctaccgggga cgatgatcat ttttagcccc agttcgcggg
     2401 cgcgctgcac tttaccaaaa agcagccccg ccacctggcg atcggcaaac gcctcgcgca
     2461 ggctgtcaat aagctgttgc tgggcctgag actccccctg caccatcgcc agcacgcgat
     2521 catactcttt catctgcaac agaccattga gagtcgacat ccaattgaga tgctcgtgtc
     2581 gcaatgtacg aaggctctca acgtattgtt ttatttgcgt cagttgcgca ttgagggtgg
     2641 aaatttcgtc tttactgcga aagctgataa tggctcccag caaatcatca cctgaacgaa
     2701 tagcttcccg attggcaata acgctcagac cgttaaagtt agccaccaca tcctgacgtt
     2761 tttcatcaat ctgttcggta aagaaatcgg cagacctgac cacttcagca atgggtttac
     2821 ccaaccattg ccgtccaggg gaactcagcc ccagcatctt tcttgcgtta cgattgatgg
     2881 cggtaatgta accgtgcgga tccaccgcaa tcagcccttc ataaaccgaa ctaaacagcg
     2941 cctcttgctg acggaccacc cgtgcgattt gctttggctc catgcccatc atctgccgac
     3001 ggatatgcgc ggccaggaac cacgacagca acatcagaat ccctaacagc acgacaaaca
     3061 cacctgccat cggtaataaa aactcagccc gccagctatc gattttactc accaggtagc
     3121 caatcgacac cacgccgatg acttttccat cgtcatcaaa gattggcgtt ttggcgcgca
     3181 tcgccatacc cattgaccct ttcccggtaa tgaagtagct ctcccctttc tccagcgcgc
     3241 cctgtttggt gaactgcata ggataaccaa ttttctccgg attaggatgg taaaggcgga
     3301 tcgagtgccg gtcgccaatc accacataat caaaatcggt atctctttgt aatttgttag
     3361 cgatggtcgc cagccgtttg tagtcacgcg ttttcaccgc agagatgaca ctgtcattgg
     3421 aggcaataat tttcgcctga ttcattgcca tgtcgcgtac atgaagcgtt aaatagtcct
     3481 caaaactggc cgtaaaatat tgcgccaggg ctgcaatgac aaatattgag aacaccagaa
     3541 tcagcaaaaa gatacgcagc ggaaatgtca gtctttggaa aaatgcaaac tgtttattct
     3601 cgttaagctg caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat
     3661 taaaatgttg aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta
     3721 aatcaattaa atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta
     3781 aaaccattaa acaaataaaa aaaccactaa ctctatgtga aataaatcaa aatttcacgc
     3841 cgaaatactc cttaggatgt atagcgaaag gagaaaaaga tatacctcga tcacccccct
     3901 ttctcccaag tgaaaataaa aggttatcag tttgcaacat tgaacaacat tcgttgcaaa
     3961 tcgataacaa catgcacctt caggatacta tttattatgt tcgtcaatga tattttcacc
     4021 cgcgtaaaac gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct gcatgaaaat
     4081 gatttgagcg ttgacaccac agtcgaagta tttattaccg taacccgcga tgaaaagctt
     4141 atcgcgtgcg gtggaattgc cggaaatatt attaaatgcg ttgctatcag tgaatccgtt
     4201 cgcggtgaag gactggcgct gacattagcc actgaactga taaacctcgc ctatgagcgg
     4261 cacagcacgc atctgtttat ttataccaaa accgaatacg aggcgctgtt ccgccagtgc
     4321 ggtttttcca cgctgacctg cgtacccggc gtgatggtgc tgatggaaaa cagcgccacg
     4381 cgactgaaac gctatgccga atcgctgaaa aaattgcgtc atccagggaa caagattggc
     4441 tgcattgtga tgaacgccaa tccctttacg aatggtcacc gttatctgat tcaacaagct
     4501 gcggcacagt gcgactggtt tcatctgttt ttagtcaaag aagattcttc acgcttcccc
     4561 tatgaagacc ggctggatct ggtgttaaaa ggcaccgccg atattccacg cctgactgtg
     4621 catcgcggct cagaatacat catctcccgc gctacgttcc cttgctactt cattaaagaa
     4681 cagagcgtca ttaaccattg ttacaccgaa attgatctga agattttccg tcagtacctc
     4741 gctcccgcgc ttggtgtaac tcaccgcttt gtcggtactg aacccttttg tcgcgttacc
     4801 gcccagtaca accaggatat gcgctactgg ctggaaacgc cgactatctc cgcagcgccc
     4861 atcgaactgg ttgaaattga gcggctgcgt taccaggaga tgccgatatc cgcttcccgg
     4921 gtacgtcaac tgctggcgaa aaacgatctc acggctatcg cgccgctggt ccctgcagtc
     4981 acgctgcatt atttgcagaa cctgcttgaa cactcccgcc aggacgcggc agctcgtcaa
     5041 aagacccccg catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc gttgcaggca
     5101 cccttgagtc tggggatgtg atgatacgca tcgccccact cgatacgcag gatatcgacc
     5161 tgcaaatcaa tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc accattctgg
     5221 acgttctcgc ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgac aaaggcgcac
     5281 tggactgcat tttacgtgca cgactggaag ccctgctggc acgcgccagc ggtatcccgg
     5341 ctctgccatg ggaggattgc caatgatttc cgcttcgctg caacaacgta aaactcgcac
     5401 ccgccgcagc atgttgtttg tgcctggtgc caatgccgcg atggtcagca actccttcat
     5461 ctacccggct gatgccctga tgtttgacct cgaagactcc gtggcattac gtgaaaaaga
     5521 caccgcccgc cgcatggttt atcacgcgct gcaacatccg ctgtatcgcg atattgaaac
     5581 cattgtgcgt gtcaacgcgc tggattccga atggggtgtt aacgacctgg aagccgtcgt
     5641 tcgcggtggt gcggacgttg tgcgtctgcc gaaaaccgat accgctcagg atgttctgga
     5701 tattgaaaaa gagatcctgc gtatagaaaa agcctgtgtt cgtgaacccg gcagcaccgg
     5761 cctgctggcg gcgattgaat ctccgctggg cattacccgc gcagtggaaa tcgctcacgc
     5821 ttccgagcgt ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca acctgcgtac
     5881 agaacgctcc ccggaaggaa ctgaactgct gttcgcacgc tgttccattt tgcaggccgc
     5941 gcgctctgcg ggtattcagg cgttcgatac cgtctattcc gacgctaaca acgaagccgg
     6001 attcctgcaa gaagccgccc acatcaaaca gctgggcttt gacggcaaat cgctgatcaa
     6061 cccgcgtcag attgatctgc tgcacaacct ctacgcaccg acccagaaag aagtggatca
     6121 cgcccgccgc gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg gcgtggtttc
     6181 cctgaacggt aagatggtgg acggtccggt tatcgatcgc gcccgtctgg tgctctcccg
     6241 tgcagaactt tccggcatcc gcgaagaata aggcaatcaa aatgacgcag aaaattgaac
     6301 aatctcaacg acaagaacgg gtagcggcct ggaatcgtcg cactgaatgc gatcttgccg
     6361 ctttccagaa ctcaccaaag caaacctacc aggctgaaaa agcgcgcgat cgcaaactgt
     6421 gcgccaacct ggaagaagcg attcgtcgct caggtttaca ggacggcatg acggtttcct
     6481 tccatcacgc tttccgtggc ggtgacctga ccgtcaatat ggtgatggac gtcatcgcga
     6541 agatgggctt taaaaacctg accctggcgt ccagctccct gagtgattgc catgcgccgc
     6601 tggtagaaca cattcgccag ggcgtggtta cccgcattta tacctctggc ctacgcggtc
     6661 cactggcgga agagatctcc cgtggtctgc tggcggaacc ggtgcagatc cactctcacg
     6721 gcggtcgtgt gcatctggta cagagcggcg aactgaatat cgacgtggct ttcctcggcg
     6781 tcccgtcctg tgatgaattc ggtaatgcca acggctacac cggtaaagcc tgctgcggct
     6841 ccctcggcta tgcaatggtt gatgccgaca acgcaaaaca ggtcgtgatg cttaccgaag
     6901 aactgctgcc ttatccgcat aatccggcaa gcattgagca agatcaggtt gatttgatcg
     6961 tcaaagttga tcgcgttggc gatgctgcaa aaatcggcgc tggtactacc cgtatgacca
     7021 ctaacccgcg cgaactgctt attgcccgta gcgctgcgga tgtgattgtc aactctggct
     7081 acttcaaaga aggtttctcc atgcaaaccg gcaccggcgg cgcttcgctg gcggtaaccc
     7141 gtttcctgga agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc ctcggcggta
     7201 ttaccgcgac gatggttgat ctgcacgaaa aaggtctgat ccgcaaactg ctggatgtgc
     7261 agagctttga cagccatgct gcgcaatcgc tggcccgtaa ccccaatcac atcgaaatca
     7321 gcgccaacca gtacgctaac tggggttcga aaggcgcatc ggttgatcgt ctcgacgtgg
     7381 tggtactgag cgcgctggaa attgacaggc agttcaacgt taacgtgctg accggttctg
     7441 acggcgtact gcgtggcgct tccggtggtc actgcgatac cgcgattgcc tctgcgcttt
     7501 ccatcatcgt cgcgccactg gtacgcggtc gtattccgac tctggtggat aacgtgctga
     7561 cctgcatcac cccgggctcc agtgtcgata ttctggtcac agaccacggt atcgcagtta
     7621 acccggcacg tccggaacta gcagaacgtc tgcaggaagc gggcattaaa gtggtttcca
     7681 ttgagtggct gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg attgaattca
     7741 ccgaccgcgt cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat gttgtgcatc
     7801 aggtgaagga ataagccatg cacctgcttc ctgaactcgc cagccaccat gcggtgtcaa
     7861 ttcccgagct gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgcc tggctcaagc
     7921 accatcctgt tccactggtc tcctttaccg tggttgcgcc tgggccgatt aaagacagcg
     7981 aggtcacacg ccgaattttt aatcatggcg tgacagcctt gcgtgcctta gccacaaagc
     8041 agggctggca aattcaggag caggctgcac tggtttccgc cagcgggccg gagggcatgt
     8101 tgagcattgc cgccccggct cgcgatctca agctcgccac cattgagctt gaacatagtc
     8161 atcctctcgg gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa attctctccc
     8221 gccgcgacta ttcactgccg cctcgccgct gcctgttgtg cgagcaaagc gcagccgtct
     8281 gcgcgcgtgg aaaaacccat caactgaccg atttactcaa ccgcatggag gcactgctga
     8341 acgatgtcga tgcctgcaac gtcaactaaa accacaaagc ttgcgacgtc attaatcgat
     8401 gagtacgccc tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc accgaaacca
     8461 ggcctcgtgg atcgcattaa ctgcggtgcg cacaaagata tggcgctgga agatttccac
     8521 cgcagcgcgc tggcgattca gggctggtta ccccgtttca ttgaatttgg tgcctgtagt
     8581 gcagaaatgg caccggaagc ggtactccac ggattacgcc caattggtat ggcttgcgaa
     8641 ggtgatatgt tccgcgccac tgcgggcgta aacacgcata aaggcagcat tttttcttta
     8701 gggctgctat gtgcggcaat tggccgtttg cttcagctca accagccggt tacgccaaca
     8761 accgtttgtt ctacggcggc aagtttctgc cgtggcctga ccgatcgcga actgcgtacc
     8821 aataattcac gactgacggc aggtcaacgg ttgtaccagc agcttggcct taccggcgca
     8881 cgcggtgaag ccgaagcggg ttatccactg gtgatcaacc acgccttgcc gcattacctc
     8941 actctgctgg atcaggggtt agatcctgaa ctggcattgc tcgatacctt gctcctactg
     9001 atggctacca acggcgatac caacgttgca tcgcgcggtg gcgagggggg cctgcgctgg
     9061 ctacagcgcg aggcgcaaac attattgcaa aaagggggca ttcgaacccc cgccgatctc
     9121 gattatctcc ggcagttcga cagggagtgt atcgaacgaa atctcagtcc aggcggcagt
     9181 gccgacctgc tgatccttac ctggttttta gcacagattt aattatttaa gcacttgata
     9241 aatttggaaa tattaatttt cggagaaccc gtatgtcttt agcaaaagat aatatatgga
     9301 aactattggc cccactggtg gtgatgggtg tcatgtttct tatccctgtc cccgacggta
     9361 tgccgccgca ggcatggcat tacttcgctg tgtttgtggc aatgattgtc ggcatgatcc
     9421 tcgagccaat tccggcaaca gcgatcagtt ttattgcggt tactatttgc gttattggca
     9481 gtaattacct gctctttgat gccaaagaat tagctgaccc agcgtttaat gcgcaaaaac
     9541 aggcgctgaa atggggcctg gctggttttt ccagcaccac ggtatggctg gtatttggcg
     9601 catttatttt tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt gcccttttcc
     9661 tggtgaaatt catggacaaa cgcacgctga cgttgggtta tgcgattgtc attatcgaca
     9721 ttctgctggc accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg gtttttccgg
     9781 tcattaaaaa cctgccgccg ttgtttaaat cattcccgaa cgatccgtcc gcgcgtcgta
     9841 ttggcggcta tttgatgtgg atgatggtca ttagtaccag tctgagttca tccatgtttg
     9901 tcaccggtgc ggcaccaaac atgctgggtc tggagttcgt cagcaaaatt gccggtatcc
     9961 agattagctg gttgcagtgg ttcctctgct tcctgccggt tggggttatc ttgcttatca
    10021 ttgcgccgtg gctttcctac gtgctgtaca aaccggaaat cacacacagt gaagaagtgg
    10081 caacctgggc gggtgatgaa ctaaaaacca tgggtgcgct gacacgcaga gagtggacgc
    10141 tgattggcct tgtattgctc agcttaggtt tgtgggtatt tggcagtgaa gtcattaatg
    10201 ctactgcggt tggtctgctg gcagtttcgc taatgctggc tctgcacgtt gtgccgtgga
    10261 aagacattac ccgctataac agcgcatgga acacgctggt caacctggca actctggttg
    10321 tgatggctaa cggcctgact cgttctggtt ttattgactg gttcgccggt accatgagta
    10381 cgcacctgga aggattctca ccaaacgcaa cggtgattgt actggttctg gtgttctact
    10441 ttgcacacta cctgtttgcc agcctgtctg cgcacaccgc aaccatgctg ccggttattc
    10501 tggccgtcgg taaaggtatt ccgggcgtac caatggaaca actgtgtatc ctgctggtgc
    10561 tgtctatcgg tatcatgggc tgtctgacgc cgtatgcaac cggtcctggg gtgattattt
    10621 acggctgtgg ctatgtgaaa tcaaaagatt actggcgtct tggcgcaatc ttcggggtga
    10681 tttacatctc tatgttactg ttggttggct ggccgattct cgccatgtgg aactaatacg
    10741 ttcgccaaac gctgaataaa tcatcacgcc cgccaggtaa gcccacctgg cgggcttttt
    10801 tatgatttaa tagatagtat tcagtgtatg tatgagttcc acacccatta tgaaagcatt
    10861 ctgccgtaac gccgcgttgc tcgcggtttc tctgcttccc ttatcttctg ccaacgccgt
    10921 agcgttgcag gcaaaacagt atggcgattt tgatcgctat gtcctggccc tctcctggca
    10981 aaccggattt tgccagagtc aacacgatcg aaatcgtaac gaacgagatg aatgtcgcct
    11041 gcaaaccgaa acgaccaaca aagctgattt tctgaccgta catggtctgt ggccaggatt
    11101 gcctaaatcg gttgctgccc gtggtgttga tgaacgccgc tggatgcgct tcggttgcgc
    11161 tactcgccca atcccgaatc taccagaagc acgcgccagc cgaatgtgtt catcgccgga
    11221 aaccgggtta tcactggaaa cggccgctaa actaagtgaa gtcatgccag gagctggcgg
    11281 acgttcctgc ctggaacgct acgaatatgc caaacacggt gcctgctttg gttttgatcc
    11341 ggacgcatac ttcggtacga tggtacgcct gaatcaagaa attaaagaga gcgaagccgg
    11401 aaaattcctt gcggataatt acggtaaaac agtgagccgc cgtgactttg acgccgcctt
    11461 tgccaaaagc tggggaaaag agaacgtgaa agcagttaag ctaacgtgcc agggtaaccc
    11521 tgcgtatttg actgaaattc agatctcgat caaagctgac gccatcaacg ctccgctttc
    11581 tgcaaactca ttcttgccac aacctcaccc aggtaactgt ggcaaaacct ttgtgattga
    11641 taaagcgggt tattaatcct ctgatttaca aaacacgcta attcgtcttc cataaccatg
    11701 ccgattaaga attatacata atgcgctgca ttgaggaatt ccctgaatgt gacgcaaatc
    11761 acttcaaaga gtgagcaaca gtcagtatca tcatgtgagt taaaccctcg ccgcctgacg
    11821 gtgagggttt tcttttgggt atgtttatct gcgacactcg cagtaccgac gacatggagt
    11881 aaaaatgtcc agaccaacta tcatcattaa cgacctggat gccgaacgca tcgatattct
    11941 gctggagcaa cccgcctatg ctggtttgcc aatcgccgac gcgttaaacg cagagttgga
    12001 tcgcgcccaa atgtgttcgc cagaagagat gccacacgac gtggtgacaa tgaacagccg
    12061 ggttaaattc cgcaatctta gagatggcga agtgcgtgtg cgcacgctgg tgtatccggc
    12121 aaaaatgacc gatagcaata ctcagctttc cgttatggct ccggtaggtg ccgcactgct
    12181 ggggctgcgc gttggcgatt ccattcactg ggaacttccg ggcggcgttg caacccacct
    12241 tgaagtgctg gaactcgaat accagccaga agctgctggc gactacctgc tttaatccca
    12301 tctgcacaag cgataactcg ctttgcccag aggatgcact cgcatcctct ttacgcaaaa
    12361 tcagcaattt ttcctggttc tcactgttca cttttgctgc aaaattattc cgtttcgttt
    12421 gtgggaataa ccatggcaga agcattttac atcctgatag gatttctgat tatggcggcg
    12481 attatcgtca tggctgtcct ttacctcgaa aaccactcct gacgatcata ttgttccccc
    12541 cggcatcgca ttcaccagac ctgaaaccgc cttctgcgcc gcctctgcgc tttgtgcacc
    12601 gggtaccaga atcaccttac ggcactcctc ttcacgtttt tcgaaaatct catatccccg
    12661 ggcggcctct tcaaacggca tatagtgggt aacaatttct tctggtttca gtaatccttt
    12721 ctcaattaac ggtaataatt ctcccagcca tgcgtgaacg tgggtctgtc ccattttaaa
    12781 cgtcaaccct ttatcaaagg cgtcgccaaa caggaaaccg tgaataaatc cagcgtagac
    12841 gcccggtacg ctaacaatgc cgccacgcct gaccgccgca atacactggc gcaacgcttt
    12901 accgctgctg ccctccagtt tcaggttagt cagcaccgtt tccgtggtgc tgcctttcgc
    12961 ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg tgacctgccg tttgttcaat
    13021 aattgactgt gccggatcgc tatcttcatc gaaattaatc gggatcgcgc cgtagcggtc
    13081 agcggcgaaa cgcaagcggt agggatgatg atcaacaaca aaaatctgtt ccgcaccgag
    13141 caaccgtgca caggcgattg tcaacaatcc cacaggacca gcaccataga ctgcaacgct
    13201 tgaaccttgt tggatctgca cattttttgc tgcctgccat gccgttggca gaatatcaga
    13261 aaggaaaagg gctttatcat ctgaaagcaa aggcggtact ttaaacggcc ccacattccc
    13321 tttagggacg cggacatatt ccgcctgccc gctaggaacg ccaccataca ggtgactata
    13381 accaaacaat gccgcgggag caggtatctg ttttttattg agcgcagcgc ctttacccgc
    13441 attggtattt tcgcaggcgg catattgctg taatcgacag aaaaaacagt cgccacaagc
    13501 aatgacgaac ggaattacca cccggtcgcc tttttgcaaa ttttttacgt ccttcccggt
    13561 ttcaacgact tccccataaa ttcatgacca aaaatatcgc catgtttaac ctggggtatt
    13621 ttgcctcgat aaagatggag gtcagagcca cagatcgccg ttaccgtaat acgcagaata
    13681 atatcatctg cctgttcaat gcccggatcg ggaacatttt ctacctgaac gtgatgtggg
    13741 ccgtgataag tcaatgcttt catgcaacct ccagagacag ttcaaaaaag agtcagtctt
    13801 taagggtagc tatcacagcg ggaaaacggg cggtaccgca ggttttggga gaattcctgg
    13861 tgttcagtga gaatatatta atcacagaaa ccgttcgtaa ggaaaaagaa ccagacaaat
    13921 atcagggata aatattataa tcatggcgaa tgatttgttc atgattaaca gggagaaagg
    13981 gttatgtata aggcaatcat tatgccagtt gatgtatttg aaatggaatt gagcgacaaa
    14041 gctgttcgcc acgctgaatt cctcgcccag gatgacggag ttattcatct acttcacgta
    14101 ctacccgggt cagccagcct gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa
    14161 gagcatctgc aacatgaagc agaagaacgt ctgcaaacga tggtcagcca cttcaccatc
    14221 gatccttccc gcattaaaca acatgtccgt tttggtagcg tgcgggatga agtcaatgag
    14281 ttggcagaag aactgggggc tgatgttgta gttatcggtt ctcgcaaccc atcgatttcg
    14341 acccatctgt taggttctaa cgcctcgagc gtgatccgcc acgccaatct gcctgtactg
    14401 gtcgttcgct aacagataaa agtatttatg ccgccctgcc tggaacatcc cggcagggct
    14461 ttttaatgcc ataaaaaagc cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa
    14521 tcttacttct tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc
    14581 tttggcacct tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc
    14641 cgctgcgaac acgcctttga cgttggtttc acatttcgca tcaatgataa tctcgcccat
    14701 gcggttacgt tcgactgcgc cttccagcca gttggtgttc gggagcagac caatctggac
    14761 aaaaataccg gccagttcga tgttgtgaat attgccgctg acacgatctc gatattccag
    14821 accaacgact ttgctgccgt cgcctttcac ttccgtggtt tgcgcattca gaataatgtc
    14881 gacgtttttc aggctgcgca gtttgtcctg cagaacctgg tcggctttca tttctggcgc
    14941 aaattccagc agcgttacgt gctcaacgat acccgccagg tcgattgccg cttccacgcc
    15001 ggagttaccg ccgccgataa ccgctacgcg tttgccttta aacagcgggc cgtcgcagtg
    15061 cgggcagtag gtcacgcctt tagtgcgata ctgatcttcg cccggaacgt tcatgttgcg
    15121 ccattttgca cccgtcgcca cgataatgct gcgtgctttc agtaccgcgc cagaagctgt
    15181 ttcaatctgg tgtagaccac cttcaaccgc tgccgggatc agtttgctgg cgctctggct
    15241 gtcgatcaca tcaacgtcgt attcatcaac gtgaactttc agtgcgcctg ccaacttctg
    15301 cccttcagtc ttcggtacag aaatgtagtt ttcgatatca acggtatcga ggatctgacc
    15361 accaaaacgt tcgcccatca gaccggtacg gatgccttta cgtgcggagt aaattgctgc
    15421 cgctgcaccc gccgggccgg aaccaacgat taatacgtca taagcatcac gcttgttcag
    15481 ctcttctgcc gcacgttttt ccgcgccagt atcaattttg gcaacgattt cagtcaacgt
    15541 catgcggccc tgaccaaact ctttcccgtt tacgaacact gccggaacgc ccatcacgtt
    15601 gcgatcggtg atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct tgatgcgcgg
    15661 gttcaatacg ctcatcaggt tcagcgcctg caccacgtcc gggcagttat ggcaagagag
    15721 cgagtaatag gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct ccagcagaga
    15781 ctgcgcttct ttcgacggat gaccaccggt ccacagcaac gccagtacca gcgaggtgaa
    15841 ctcgtggccc agcggggagc ctgcaaaacg tggtccctgg ttggaacctg ggttggtgat
    15901 caggaaagac ggcttacgca ctggcaagct gttatcttct ttaaaggtga ctttgtctga
    15961 cagttctgcg atttcagcca acagttcctt gatttctgcc gatttagcgc tgtcatccag
    16021 cgtggcaatt aactcaacag gcttggtcaa tttctcaagg taagccttga gttgagtttt
    16081 catatttgtg tcgagcatgt ttatctcctg ggctttaaac atcatcatgc aagcggcctc
    16141 tgcggctacc tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca
    16201 cccgaagaat taggtgaatt tccgaagtat ttcgcgtcag agcaaggcga ctagcctgtg
    16261 aatccccggg agcttacaca agtaagtgac cggggtgagc gggcgacgcc aacgccgcta
    16321 tggcgtgaaa gacgaaggaa atttagattt taccaaccag gtccagagac ggagccagag
    16381 ttgcttcacc ttctttccat ttagccgggc aaacttcacc tgggtgagaa gctacgtact
    16441 gagctgcttt gattttacgc agcagatcag acgcgtcacg gccaatgcct tcagcggtaa
    16501 cttcgattgc ctggatgata ccctgcgggt caacaacgaa ggtcgcacgg tcagccagac
    16561 cttcatcttc acgcatgttg tcgaagttac gggtcagggc gccagtcggg tcgccgatca
    16621 tcgcatattt gattttagcg atggtttcag agctgctgtg ccatgctttg tgggtgaagt
    16681 gagtatcggt agatactgcg tatacgtcta cgcccagttt ctgcagttct tcgtagtggt
    16741 cagcaacgtc acccagttcg gtcgggcata cgaaagtaaa gtcagccggg tagaagaaga
    16801 agacgctcca gcggccttcg gtatcttttt cggtgatttc gatgaattcg ccgtttttga
    16861 atgcctggtt tttaaaaggt ttaattttgg tgttaatcaa ggacatctat acttcctccg
    16921 tgttttcgat gagatgtaag gtaaccaatt tttgccgatt cggctaatgc gtttccatta
    16981 tcaaatcgat aagttttacc ttacaacctt cgtaagacaa ctttgtgaac tttgcaccgc
    17041 cagcggcgat aaaaagtaaa atggccccct ttccaggtga ccgtgtaatc tgaactaccc
    17101 tttggtatct tcagcgcctg cctgcaggct gaggtgttgc ccttacaaat gcaacaacga
    17161 catggattac aacaccctca taaacaaagg gcaatcacct gatctaagct cttacctatg
    17221 acagtgatag gttatgcctt ttactcgact tttgcactga ctgaaaagaa caaattaatg
    17281 ttaaaaaaga tacttttact ggctctgctt cctgcaatcg ccttcgcaga ggaacttcct
    17341 tccccagtaa aagcgattga aaaacatggc attacaatca tcaaaacgtt cgatgccccc
    17401 ggaggaatga aaggttatct cggaaagtat caggatatgg gcgtcaccat ctacctgact
    17461 cccgatggta agcacgctat ctctggttac atgtacaacg agaaaggtga aaacctgagt
    17521 aacacactta tcgaaaaaga aatttacgca ccagccggac gcgaaatgtg gcaacggatg
    17581 gaacaatccc actggctcct cgacggtaaa aaagatgcgc cggtcattgt ctacgtcttc
    17641 gccgatccgt tctgcccata ttgtaaacag ttctggcagc aggcgcgccc gtgggtagat
    17701 tctggcaaag tgcaattaag aacattgttg gttggggtta tcaagccaga aagcccggcg
    17761 acagcagcgg caattcttgc ctccaaagat cccgcaaaaa cctggcaaca atatgaagcc
    17821 tctggttgca agcttaagct aaacgtgcct gcaaacgtaa gtacagagca aatgaaagtg
    17881 ttaagtgaca atgagaaact gatggacgat ctgggggcaa atgtcacgcc ggctatctat
    17941 tacatgagta aggaaaatac gctgcaacag gccgtggggt tgcccgatca gaaaacgctt
    18001 aatatcatta tgggaaataa ataaaaggta attcatggat ggttgaacta tatccctgct
    18061 tttcaaccat cctaaaaaag catgaattgc tcgctctacc ctaagtggct gtgctatttt
    18121 agaaaaactt aatcctgagt taagcaaaaa aacataatca ataaaaatac atacgcaaaa
    18181 aacattgatt aagtgaatat accatggaag aaaaatataa accggagtag tgtatggcca
    18241 atctctacga cttgaaaaag ttcgacttaa atcttcttgt catatttgag tgtatttacc
    18301 agcatttaag cattagcaaa gctgctgaat cactatatat caccccatca gccgtgagcc
    18361 agtcgttaca acgcttacgt gcgcaattta atgacccttt attcattcgc tcaggaaaag
    18421 gtatcgcgcc gacgacaaca gggttgaatc tgcatcacca tcttgagaaa aacctcaaag
    18481 gccttgaaca aacaattaat atagtcaata agtccgaact aaagaaaaac tttattatct
    18541 acggccctca gctcatttct tgctctaata atagtatgct gatccgctgc ctacggcaag
    18601 atgcctctgt cgagattgag tgccatgata tcctcatgtc agcagagaat gctgaagaac
    18661 tgttggttca ccgcaaagcc gatctggtga tcacccaaat gccagtgatc agccgttccg
    18721 tcatttgcat gccattgcat acaattcgca atactctaat ttgtagtaat aagcatccgc
    18781 gaattactga caatagcact tatgaacaaa ttatggcaga agagttcacc caattaatat
    18841 ccaaatcagc aggagttgat gatattcaaa tggaaataga cgagaagttt atgaatcgca
    18901 aaatttcatt tcgtggttcc tctttgctga caataattaa tagcattgca gttacagatt
    18961 tactgggaat agtcccctat gaactttata actaccaccg tgactttctt aatttaaaag
    19021 agataaaact agaacatccg ttaccttcaa taaagcttta catttcctat aataaatctt
    19081 ctttaaataa tttggtgttc tcccgattta ttgaccgtct aaatgagagt ttttgatata
    19141 taactacaga cagctactat aactttcatc tatttattca cagctcgaat gaaaaataaa
    19201 acactctgct ttacagtgtt tactttataa ttaacagtct tatctcactg ttcaccctgt
    19261 aacgttcaga gagcgtcttc agatgtctat ttataaaatt cctcttccac tcaatattct
    19321 ggaagctgca cgagagcgta ttacctagac actcaacacc ctaccccgcg tttgtgtctc
    19381 attttctggc ggcaaagact ctggtttaat gctgcatctg acagccgaac ttgcccgaca
    19441 aatgggcaaa aaaatctgcg ttttgtttat cgactgggag gc
//
LOCUS       DAJTNM010000074        19342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTNM010000074 DAJTNM010000000
VERSION     DAJTNM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.074"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..1828)
                     /gene="glmS"
                     /locus_tag="OO172_002626"
     CDS             complement(<1..1828)
                     /gene="glmS"
                     /locus_tag="OO172_002626"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5512761.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(1990..3360)
                     /gene="glmU"
                     /locus_tag="OO172_002627"
     CDS             complement(1990..3360)
                     /gene="glmU"
                     /locus_tag="OO172_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5512762.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGAGMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3712..4131)
                     /gene="atpC"
                     /locus_tag="OO172_002628"
     CDS             complement(3712..4131)
                     /gene="atpC"
                     /locus_tag="OO172_002628"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5512763.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4152..5534)
                     /gene="atpD"
                     /locus_tag="OO172_002629"
     CDS             complement(4152..5534)
                     /gene="atpD"
                     /locus_tag="OO172_002629"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5512764.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5561..6424)
                     /gene="atpG"
                     /locus_tag="OO172_002630"
     CDS             complement(5561..6424)
                     /gene="atpG"
                     /locus_tag="OO172_002630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5512765.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6475..8016)
                     /gene="atpA"
                     /locus_tag="OO172_002631"
     CDS             complement(6475..8016)
                     /gene="atpA"
                     /locus_tag="OO172_002631"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5512766.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8029..8562)
                     /gene="atpH"
                     /locus_tag="OO172_002632"
     CDS             complement(8029..8562)
                     /gene="atpH"
                     /locus_tag="OO172_002632"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5512767.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8577..9047)
                     /gene="atpF"
                     /locus_tag="OO172_002633"
     CDS             complement(8577..9047)
                     /gene="atpF"
                     /locus_tag="OO172_002633"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5512768.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9109..9348)
                     /gene="atpE"
                     /locus_tag="OO172_002634"
     CDS             complement(9109..9348)
                     /gene="atpE"
                     /locus_tag="OO172_002634"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5512769.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9395..10210)
                     /gene="atpB"
                     /locus_tag="OO172_002635"
     CDS             complement(9395..10210)
                     /gene="atpB"
                     /locus_tag="OO172_002635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5512770.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10219..10599)
                     /gene="atpI"
                     /locus_tag="OO172_002636"
     CDS             complement(10219..10599)
                     /gene="atpI"
                     /locus_tag="OO172_002636"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5512771.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11216..11839)
                     /gene="rsmG"
                     /locus_tag="OO172_002637"
     CDS             complement(11216..11839)
                     /gene="rsmG"
                     /locus_tag="OO172_002637"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5512772.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11903..13792)
                     /gene="mnmG"
                     /locus_tag="OO172_002638"
     CDS             complement(11903..13792)
                     /gene="mnmG"
                     /locus_tag="OO172_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5512773.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14171..14614)
                     /gene="mioC"
                     /locus_tag="OO172_002639"
     CDS             complement(14171..14614)
                     /gene="mioC"
                     /locus_tag="OO172_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5512774.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14704..15162)
                     /gene="asnC"
                     /locus_tag="OO172_002640"
     CDS             complement(14704..15162)
                     /gene="asnC"
                     /locus_tag="OO172_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5512775.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15314..16306
                     /gene="asnA"
                     /locus_tag="OO172_002641"
     CDS             15314..16306
                     /gene="asnA"
                     /locus_tag="OO172_002641"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5512776.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16311..17762)
                     /gene="viaA"
                     /locus_tag="OO172_002642"
                     /pseudo
     CDS             complement(16311..17762)
                     /gene="viaA"
                     /locus_tag="OO172_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17756..19252)
                     /gene="ravA"
                     /locus_tag="OO172_002643"
     CDS             complement(17756..19252)
                     /gene="ravA"
                     /locus_tag="OO172_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5512777.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4620 a         5116 c         4855 g         4751 t
ORIGIN      
        1 actcaaccgt aaccgatttt gccaggttac gcggctggtc aacgtcggtg cctttgatca
       61 gcgcgacatg gtaagccagc agctgcagcg gaacggtgta gaagatcggt gcaatcacct
      121 cttccacatg cggcatctcg atgatgtgca tgttatcgct acttacaaaa cccgcatcct
      181 gatcggcgaa gacatacaat tgaccgccac gcgcgcgaac ttcttcaatg ttggatttca
      241 gtttttccaa cagttcgttg ttcggtgcaa cgacgataac cggcatatcc gcgtcaatca
      301 gcgccagcgg gccgtgtttc agctcgcctg cagcgtaggc ttcagcgtga atgtaagaga
      361 tctctttcag cttcaatgcg ccttccagcg cgattgggta ctgatcgcca cggcccagga
      421 acagcgcgtg atgtttgtca gagaaatctt ctgccagagc ttcaatgcgt ttatcctgag
      481 acagcatctg ctcaatacgg ctcggcagcg cctgcagacc atgcacaatg tcatgttcaa
      541 tggaggcatc cagacctttc aggcgagaca gcttcgccac cagcatcaac agcacagtta
      601 actgagtggt gaatgcttta gtggacgcca cgccgatttc cgtacccgcg ttggtcatca
      661 gcgccagatc ggattcgcgc accagagaag aacccggaac gttacagatt gccagcgaac
      721 caaggtaacc cagctctttc gacagacgca ggcccgccag ggtatccgcg gtttcgccag
      781 actgtgacaa ggtgatcatc aggctgttac gacgcacggc agatttacga tagcggaatt
      841 cagaggcgat ttcgacgtcg cacggaatac ctgccagcga ttcaaaccag tagcgggaaa
      901 ccataccgga gttataagaa gtaccacagg cgaggatctg aatatgctca accttcgaca
      961 gcagttcgtc ggcgttcggt cccagctcgc ttaaatcaac ctgaccgtgg ctgatgcgcc
     1021 cggtaagggt gttttttatc gcgttcggct gttcgtagat ctctttctgc atgtagtgac
     1081 ggtaaatacc tttatcgccc gcgtcatatt gcagattgga ttcgatatcc tgacgtttta
     1141 cttccgcgcc agttttatcg aagatgttta ccgaacggcg agtgatttcc gcaatatcgc
     1201 cctcttcaag gaagataaag cgacgggtca ccggcaacag cgccagctgg tcagaagcga
     1261 taaagttttc gcccataccc aggccaatca ccagcggact accagaacgt gccgccagca
     1321 gggtatccgg gtgacgggag tccatgatca ctgtaccgta cgcaccgcgc agctgcggga
     1381 tagcacgcag aacagcctca cgcagagtcc cgccttgttt cagctcccag ttcaccagat
     1441 gggcaatcac ttcggtgtcg gtttcagaaa cgaaggtata gccacgcgct tttaactctt
     1501 cacgcagcgg ttcatggttt tcgatgatgc cattatgcac caccacaatg tgttcagaaa
     1561 catgcggatg cgcgttagct tctgaaggtt caccgtgggt cgcccagcga gtgtgagcaa
     1621 taccggtgcc gccatgcaga ggatgttctt ccgctgcctg agccagcatc tggactttac
     1681 cgaggcgacg caggcgagtc atatgacctt ccgcatcaac aacggccaga ccggcagagt
     1741 catatccgcg gtattccaga cgacgtaaac cttcaagaag gatttctgct acatcacgtt
     1801 gcgcgatcgc gccaacaatt ccacacatag tttttgattc cgatttatat cgttgtcggt
     1861 caacctgtat gcccgttttt tcgggcgccc cgagccttgt agagagtggg gttattttta
     1921 tagttactgc ttgtgggagg gagattatgt tatctcctca tcccagatga ccggattacc
     1981 cggccagaat tactttttct ttaccggacg acgccagcct tctttctgag tctgcggcac
     2041 acggctgata gctaatgcat tttcgccgac attacgcgtc acagttgtac ccgcagcaat
     2101 ggtcgcgcct ttgcctactg ttaccggggc caccagctga gtgtcggaac cgacaaacac
     2161 atcgtcgccg ataatggtct taaatttatt cgcaccatcg tagttgcagg taatggttcc
     2221 cgcgccgatg ttaacgttat cgccaatttc cgcatcgcct aggtaagtca gatgcccggc
     2281 tttcgtgcct ttacccagac gcgctttttt catctcaacg aagttaccaa cgtgtgcacc
     2341 ttccagcaac tcagcaccag gacgcagacg ggcaaacggg ccaatagtac aggccgctgc
     2401 cagattcgcg tcttccacaa cggtatacgg actgatttcg caatcatcgc caatcacgct
     2461 gtttttaatc acgcaaccgg tgccgatttt cacgcgatga ccgagagtca cgttgccctc
     2521 gatgataacg ttagtatcaa tttcaacatc gcgcccgtga gtaagcgtac cgcgcagatc
     2581 aaaacgcgcc ggatcgcgca gcataacgcc tgctaacagc agtttttcag cctgttcgga
     2641 ctggtaaacg cgctccagac gggagagttg caggcggtta ttcacacctt ctacttcgct
     2701 taaacgttgc ggatgaacgg cgacgatttc acgcccttcc tgatacgcca gcgcaataat
     2761 gtcggtgatg tagtattcgc cctgagcatt attgttggtc agcttcgcca gccagcgttt
     2821 catacctgcg ccgttggcaa tcagaatgcc ggtgttgatc tcctgaatct gacgctgctc
     2881 gtcggtggca tctttgtgct caacaatgcc ggtaactttg ccattttcac gggtgatacg
     2941 tccataaccg gtcggatcat ccagtttcac cgtcagcaga ccaatgccac cctggggttt
     3001 agcatcacgc aggcgctgga gtgtttcgac agagatcagc ggcacgtcgc cgtagagcat
     3061 taaaatgtct tcatcatcgg caaagaaagg tgcggcctgc tgcattgcat gacccgtacc
     3121 cagctgctct gcctgaagca cccagttaag gttgtcgtct ttcagcgcct gtttaagcag
     3181 atcgccgccg tgaccgtaca ccaggtgaac gtgcgctgcg cctaattcat tcgcagcatc
     3241 aatgacatgc tgaaccatcg ctttcccggc aagggtatgc agcactttcg gaagatcgga
     3301 atacatgcgc gtgcctttgc ctgcggcaag gatcactacg ctcatagcat tattcaacat
     3361 acgcgtcctg actgtaattt gagaacgaat ttaaaccgct tcaccttgaa aaaactacat
     3421 ttttttcatc gtgaaatgga cagaggataa attgttcaat aacggattat ccccgctgac
     3481 gaaagtgcca ttttcgacca tcgtcacgcc ttttgtctgc agaaaattaa ggcagtacct
     3541 tcatttaagc gtcaggtgga tgtttttgct cttatttcga tcaatgaata aacgggaaaa
     3601 caggggggtt ttatatcagc aggatctatg tgaacgctct tcaggacggg tcaacgcgta
     3661 aaaaaaagcc agcctgtttc cagactggct tttgtgcttt tcaagccggt gttacatcgc
     3721 ttttttggtc aactcgataa cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc
     3781 ctgagcgtaa tctacgtcgc cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc
     3841 ttccatggct cgcgcttcgt cgagatcctg gccgcgaatt gcagtgtcgg ccagaacggt
     3901 cacgttgcca ggctgcactt caagaatgcc gccagacaga tagataaact cttcgtgacc
     3961 gtgctgtttc acgatgcgaa tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc
     4021 agggtagatc cccagttcac cttcgctacc cgttacctgg attttctcga ccagaccaga
     4081 gaacatttgt tgctctgcgc tgacgacgtc caggtggtaa gtcattgcca tatcaccctc
     4141 cgattaaggc gttaaagttt tttggctttt tccacagctt cttcgatgga accgaccatg
     4201 tagaacgcct gctccggcag gtgatcgtat tcgccttcca tgatgccttt aaagccacgg
     4261 atggtgtctt tcagggagac gtatttaccc ggagaaccgg tgaatacttc tgccacgaag
     4321 aacggctggg acaggaagcg ctggatctta cgagcacgcg ctaccaccag tttgtcttct
     4381 tcagacagtt catccatacc caggatggcg atgatgtctt tcagttcctg ataacgttgc
     4441 aggatggact gaacgccacg cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc
     4501 agctgacggc tggtggagtc cagcgggtca acggccgggt agatacccag agacgcgatc
     4561 tgacggctca gtaccacggt tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca
     4621 gtcaagtcat ccgcaggtac gtataccgcc tgtacggagg tgatagaacc agttttggtg
     4681 gaggtgatac gttcctgcag aacgcccatc tcttccgcca gggtcggctg ataacctacc
     4741 gctgaaggca tacggcccag cagtgcggat acttccgtac cggccagggt gtaacgatag
     4801 atgttgtcaa cgaacagcag aacgtcacga ccttcgtcac ggaatttctc agccatggtc
     4861 agaccggtca gagcaacgcg cagacggttt cccggcggct cgttcatctg gccatacacc
     4921 agggatactt tgtcgataac gttggagtcg gtcatttcgt ggtagaagtc gttaccctca
     4981 cgagtacgtt cacctacgcc cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta
     5041 cgaatgagct ccatcatgtt tacggtttta cctacacccg caccaccgaa cagaccaact
     5101 ttaccgccct tagcgaacgg acacatcagg tcgataactt tgataccggt ttccagcagt
     5161 tcctgagagt ttgacagctc ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct
     5221 tcttcaccga tctcgccttt catgtcgacc ggttcaccca gtacgttcat gatacggccc
     5281 agagtcgctt tacctaccgg gacttcgatc gggtgttcga ggtcttttac atccagaccg
     5341 cgacgcagac cgtcggagga acccattgcg atggtacgca cgataccgcc gccgagctgc
     5401 tgctgaactt ccagcaccag acgctcatta ccattttgca cctcaagagc atcgtacacg
     5461 cgcggtacgg catcctgagg gaattcgacg tcaactacgg cgccgattac ctggacaatc
     5521 tttccagtag ccatcttaaa tcctctacga aataacctgt ttaaaccgcg gcggcccccg
     5581 agacgatctc ggtgagttcc tgagtaatgc tggcctgacg agctttgttg tataccaact
     5641 gcagctcttt aatcaggctg ccgccattgt cggtcgcggc tttcatcgcc accatacggg
     5701 cggcctgctc gctggccagg ttttcaacca cgccctgata aacctgagat tcgacataac
     5761 gacgcagcag ggtatccagc aacgccttcg gatcgggttc gtacaggtaa tcccaggatt
     5821 tatgtttcag atcatcatca tctgatgccg gtaacggcag cagctggctg atggtcggaa
     5881 cctgagacat ggtgttaata aatttgttgc tgacaatgta aagcttgtcc agacggcctt
     5941 cgtcgtaggc ctgcaacatc acttttaccg gaccgatcag ttcggacagg gaagggttat
     6001 cccccatgcc ggtgacctgg gcaacaacat tgccgcccac ggagttgaag aacgacacgc
     6061 ctttcgagcc gatcattgcg aggtcgcatt gaacgccttt gtcggtccag gtcttcattt
     6121 ccgccagcag ttttttgaac aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg
     6181 acaccaccag gtagcccacg cgtttaacgt cgcggtcttc caggtaaggg tgcttatatt
     6241 ccagattacc gtgtgcaagg tgaccaatca ctttgcgcat ggtttctgca taaggacggc
     6301 tggccgccat gcgatcctgc gatttacgca ttttggaagc ggcgaccatc tccatcgctt
     6361 tagtgatctt ttgcgtgttc tggacgcttg cgatcttact acgtatctct tttgcgccgg
     6421 ccatgagctt ctcctcaatg ccttgcggcc tgccctaagg caagccgcca gacgttacca
     6481 ggattgggtt gctttgaagg aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt
     6541 gtagccaccg gtctggttga tctcttgcat caacggagcg tgatcacggt cgacgtaagc
     6601 cagcagagcg gcttcgaagc tgccaatttt cgacagttca acatccgcca ggtaaccacg
     6661 ttctgctgcg aacagaacca gagactgctg cgcaacggac atcggcgcat actgtttctg
     6721 tttcagcagt tcggtcactt tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag
     6781 gtcggatgca aactgagaga acgctgccag ttcacgatac tgtgccagag cggtacggat
     6841 accaccggac agttttttca tgatcttggt ctgtgctgca ccaccaacac gggatacgga
     6901 aatacccggg ttaaccgcag gacgaatacc ggcgttgaac aggttggttt ccaggaagat
     6961 ctgaccatcg gtaatggaga ttacgttggt cggaacgaac gcagaaacgt cacccgcctg
     7021 agtttcgata atcggcagtg cggtcagaga accggttttc cctttcactt cacctttggt
     7081 gaaggcttca acgtattcgg cgttaacacg tgcagcacgc tccagcagac gagagtggag
     7141 gtagaaaacg tcgcccggga atgcttcacg tcctggcgga cgacggagca gcagggagat
     7201 ctgacggtaa gcaacagcct gtttagacag gtcatcgtaa atgatcagcg catcttcacc
     7261 gcggtcacgg aagtattcgc ccattgcgca accggcatac ggtgccaggt attgcagtgc
     7321 agcggattca gacgcggttg ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc
     7381 cagtttacgt accacgttag aaatggtgga cgctttctgg ccgatagcga catagataca
     7441 tttgataccg gaatcgcgct ggttgatgat ggcatcgata gccagtgcgg ttttacctgt
     7501 ctgacggtca ccgatgatca attcacgctg accacgaccg attgggatca tggagtcaac
     7561 ggctttataa ccggtctgta ccggctgatc tacggactga cgttcgataa cgcccggagc
     7621 gattgcttct acagcagaga agccgtcgtg atccagcgga cctttaccgt cgattggtgc
     7681 acccagagtg ttaaccacac ggcccagcag gccacggcca accggaactt ccaggatacg
     7741 gccagtacac ttaactttca tgccttcggc aaggtcagcg tacggaccca taacaaccgc
     7801 acctacagag tcgcgctcga ggttcagtgc gatagcgtaa cggttacccg gcagggagat
     7861 catttcaccc tgcatacaat cggccaggcc gtgaatgcgg ataacaccgt cacttacaga
     7921 aacaatagta ccttcgttgt gagcttcact cacaacattg aactgagcaa tgcgctgctt
     7981 gatcagttcg ctgatttcgg tggaattcag ttgcatgctc cagtcccctt aagactgcaa
     8041 gacgtctgca aggcgctcaa gacgaccgcg tacgctgcca tcaatgacca tatcacccgc
     8101 tcggatgata acgcctgcca ttacagactt atcgattttg caattcagct taactttgcg
     8161 tgacagacgt ttttccatcg cagcagaaat tttcgcgagc tgttgttcac tcagtgcggc
     8221 agcggaaatg acgtctacct cagcggtagc ctcactcacg gcacgaaggt gaataaactg
     8281 ctccagaaca tccgggagcg cgttaagacg accattttca gccattaccc gaatcaggtt
     8341 ctgaccgttt tcgtccagtt gctcaccaca aactgcgata aacgactcgg cgagcgtttc
     8401 tggcgcaagc gcgccagaga gaagctctgc catttgttcg tttttggtta cctcggcggc
     8461 aaacgccagc atgtcctgcc agcgttctac actttggtgt tcgacggcaa agtcaaaagc
     8521 tgctttggcg taggggcgag ctaccgtaat aaattcagac atcagcccct ccctccttac
     8581 agttcagcga caagtttatc cacgatgtcg ctgttagcag cttcatccac ggaacgttcg
     8641 atgatcttct cggcgccagc aacagccagg atagcaactt gcttacgcag ctcttcacgg
     8701 gcacgtttac gctcggcttc aatttcagcc tgcgcctggg ccacgatttt agtacgttcc
     8761 tgttctgcct cagctttcgc ttcgtccaga atctgcgagc ggagtttatt cgcctgctcg
     8821 atgattacct gggcttccgc tttcgctttt ttcagctggt cggtcgcgct ggcctttgca
     8881 aggtcaaggt ccttatgtgc tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga
     8941 cgtttttcga tggctgccat taatggcggc catacgtact tcatgcagaa cagaacgaac
     9001 aggacaaacg cgatggcctg gccgaggatt gttgcgttaa gattcacagc acaatgcctc
     9061 tatttagtta acgttctgat attgctcttt aaataaaagc aacgcttact acgcgacagc
     9121 gaacatcacg tacagaccca gacctacagc gatcatcggg atagcatcca ccagacccat
     9181 aacgataaag aactgagtac gcagcagagg aatcagatca ggttgacgcg ctgcgccttc
     9241 caggaattta cccccgagga tgccgatacc gatcgcagca ccgattgccg ccagacccat
     9301 catcacagcg gcagccatgt acagcagatc catattcagg ttttccatga cagtctccag
     9361 tttgtttcag ttaaaacgta gtagtgttgg taaattaatg ttcttcagac gccatcgaca
     9421 gatagacgat cgtcagaacc atgaagatga aggcttgcag cgtaatgatc aggatgtgga
     9481 aaatggccca cggcacattc aggatccact gtgaccacca cggcaacaga ccagcaatca
     9541 gaatgaaaat cagctcaccg gcatacatgt taccgaacag tcgcaaaccg agtgaaactg
     9601 gtttggacag caggcttacc ccttcaagga ttaagttgac aggaatgaac gcccagtgat
     9661 tgaacggctg cagcgtcaac tctttcgtga agccgccgat gcctttcatt ttgatgttgt
     9721 agaacagaat caggataaat acgcccagtg ccatagacag cgttacgttc acgtccgcag
     9781 acggaaccac acgcagtgca ggcagaccca gtacatgttc agcaatgtac ggcagcaagt
     9841 cgataggcag taaatccatc agattcatca ggaataccca gacgaagatc gtcagggcca
     9901 gcggagcaat cagcttgctt ttgccatggt acatgtcttt cacgctacca ttaacaaagc
     9961 cgatcaccag ctcaatcgcg gtctgaaact tacctggcac accgctggtc gcctttttgg
    10021 ctacgctacg gaataaaacc aagaacaaca gacccagcac caccgagaag aacatggagt
    10081 caatattgat tgtccagaag gtggctgggg ggttttgtgg atccaccagc gagaatgtac
    10141 gcaggtccag ctgaaggtta ttcaggtggt gtcctatgta atcctgcggc gtcatatttt
    10201 ctgaagccat gatgcctttt accctttgtt gttaattaca gccggtgcca gtatctgaac
    10261 caccagcacc aaaacccacg taacgatcag cggcaagaat accgccttta aaaccgccaa
    10321 cgccaccacc agtaacacca acatcgccag aactttgaaa gcttcgccaa atgcgaatgt
    10381 ccaagccacc cggcctttcg ctggtgtatg cgcctggtga cgccaggcaa atatcataaa
    10441 caaaacgtta ggcagaaaga ctgccaggcc cccgcttatt gcagagacgc cccagaaggg
    10501 gtctttgagg ctgaacagca atccacttgc tatcaccacc agtaactgaa cgagcagaag
    10561 cttccgagca acgtttcgac tcacgagcga cacagacatc acgtttttca ctcctgctcc
    10621 cttcgaggta tgccgcgtgt cgtataaaac tttctttaag gcttagagtc aagcatcaaa
    10681 aagcggtcaa attatacggt gcgcccccgt gatttcaaac aataagtagc caaaaggtga
    10741 ataaatgttt aaatattttt ccagtgcata caattgcgac ttttccgcta cccctgttcg
    10801 atcatgaaaa actgtaaata acgcgtaaac actggtgata aagcgtgctt cagatcacat
    10861 attgcgcatg ttcgcgcaca gcatatttat ttacttggca aatgatgcct ttgcaagttt
    10921 atgatatttc agtctaaaaa cagatactgt tttaataaat gacatttaca caacaaaaac
    10981 cacccattga catttttaat aatgttttaa cagccaatga tggttcttag cgccgatttt
    11041 tagcagactg atattttcac taatgactta ttttctgctt accaaaaaaa gccacgttat
    11101 cttgttgatg caaaagagtg aacgtggcgt taaatgtaac cagttatatc agtagaaaac
    11161 ctggttgttg ttaacagtct aaccggtcaa ttttttatga tttttttgat aaaaattaaa
    11221 ttttatttgc tttaatcacc accagatgac gttcgccatc cagggctgga acctgaagtt
    11281 taaccactga ttcgacctga tattcttcgg gcaacaaagc gatttcatct tccggcattt
    11341 gccctttcag cgcgtagaaa cggccttgct caccaggaag atggtggcac cagctcacca
    11401 tatcgttcag agaggcaaaa gcgcggctaa ttacgccatc aaatggcggc tctgaaggaa
    11461 actcttctac cctgctctgt actggttcaa tattctccag tttaagctca tgttgcacct
    11521 gacgaaggaa acgcacgcgt ttaccaaggc tatccaacag agtgaaatgg gcttcaggac
    11581 gcacgataga gagtggaatg cctggcagtc ctggtccggt gccgacatcg ataaaccgtt
    11641 caccttgcag atacggtgcc accacaatgc tatcgagaat atggcgtacc agcatctcat
    11701 taggatcgcg gaccgaagtc aggttgtacg ctttgttcca tttatgcagc atattcacgt
    11761 aggcaataag ctggtttttc tggtgatcag taagcgaaat acctgcgtct ttcagcagta
    11821 aggagagttt gttgagcacg gtgattacct gttcttgatg cgttgcttgg taagcgggtg
    11881 cttaccaggc atttttaatg cgttatgcgc tacgacgcag cataccctgt tttttcagcc
    11941 acaccagcag aatggagatg gccgcaggcg tgacgccaga aatacgcgaa gcctggccga
    12001 tagaggccgg tttgtgatcg ttaagtttgg cgatcacttc gttagaaaga ccggatacct
    12061 ggcggtaatc cagtgtcgcg ggtagcaggg tgttctcgtt acgcagctgc ttttcgatct
    12121 catcttgctg gcgcgcgata taaccttcgt atttaacctg aatctcaacc tgttccgccg
    12181 cctgttcgtc tgtcaacgca ggggcaaacg gcgtcagcgt ggttaatttt tcataagtca
    12241 tttccggacg acgcagcaga tcttcaccac tggcttcacg ggaaagcggc gcagtcaggt
    12301 gagcattcac ttcggctgca gcttccgccg acggggttac ccaggtcgat ttcagacgct
    12361 gacgctcacg ctcgatattc tcaagtttct cgttaaagcg cgcccaacgt tcgtcatcca
    12421 ccaggcccag ttcacgaccg atttcagtca aacgcagatc cgcattatct tcgcgcagca
    12481 tcagacgata ttctgcgcgc gaagtaaaca tacgatacgg ttctttggtt cctaaagtgc
    12541 acaggtcatc aaccagtacg ccgagatacg cctgagaacg tgccggagcc caaccttctt
    12601 tgtcagcaga cagacgggca gcgttaagac cggccagcaa accttgcgca gcggcttctt
    12661 cgtaaccggt agtgccgtta atctgaccag caaagaacag cccctggata aacttgctct
    12721 ccagcgtcgg tttcaggtcg cgaggatcga agaagtcata ctcaatggca taacccggac
    12781 gcacgatctt cgcgttttcc atcccctgca tagagcggac gatttgcatc tgcacatcga
    12841 acggcaggct ggtggagata ccgttcggat aaatttcatt agaggtcagt ccttccggtt
    12901 caaggaagat ctgatgctga tttctgtctg cgaagcgcat gactttgtct tcgatcgacg
    12961 ggcagtagcg tgggccgaca ccttcgatca cccctgcata cattgggcta cgatcgaggt
    13021 tactgcggat cacatcatgg gttttctcgt tggtatgagt gatataacac ggcacctgct
    13081 ggggatgcag ggacgcattg cccataaacg agaataccgg cattgggtta tcgccatgtt
    13141 gttgcgccag cacgctaaag tcgatggttc gagcatcaat acgcggcgga gtaccggttt
    13201 tcagacgacc aacgcgcagc ggcagttcac gcaaacggcg agagagcgga atggatggcg
    13261 gatcgccagc acggccaccg ctgtaattat ccagaccgat atgaattttg ccgtcgagga
    13321 acgtcccaac ggtgagcacc acagctttgg cacggaactt cagtcccatt tgggtaacag
    13381 caccgaccac gcgatcgttt tcgacaataa gatcttcaac cgcctgctgg aagatcatca
    13441 ggttcggttg gttctccagc gccgtacgta ccgcctgacg gtagcgcaca cgatccgcct
    13501 gagctcgggt agcgcgaacc gccggtcctt tgcttgcgtt tagtatccta aactggatac
    13561 ccgcttgatc gatcgctttc gccatcagac cgccgagtgc atccacttct tttaccagat
    13621 gtcccttccc aataccgccg atcgccgggt tgcagctcat ctgccccaga gtgtcgatat
    13681 tgtgtgtcaa aagcagagtc tgttgaccca tacgcgccgc agccatcgcg gcctcggtgc
    13741 ctgcatgacc cccgccaatg atgatgacgt caaaaggatc cggataaaac atggtgattg
    13801 cctcgcataa cgcggtatga aaatggattg aagcccgggc cgtggattct actcaacttt
    13861 gtcggcttga gaaagacctg ggatcctggg tattaaaaag aagatctatt tatttagaga
    13921 tctgttctat tgtgatctct tattaggatc gcactgccct gtggataaca aggatccgac
    13981 ttttaagatc aacaacctgg aaaggatcat taactgtgaa tgatcggtga tcctggaccg
    14041 tataagctgg gatcagaatg aggggttata cacaactcaa aaactgaaca acggttgttc
    14101 tttggataac taccggttga tccaagcttc ctgacagagt tatccacagt agatcgcacg
    14161 atctgtatac ttatttgagt aaattaatcc acgatcccag ccattcttct gccggatctt
    14221 ccggaatgtc gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg
    14281 aatttttgag ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac
    14341 tgccaatacc gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg
    14401 cttcatagaa aggagaaagg ttgtccggaa tatctccggc accgtgggtg gagctgataa
    14461 ccagccagat ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg
    14521 taaaacccgc ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga
    14581 gggtgctgcc gctgataaga gtgatatctg ccataaaccg ccacctttat taagagtggc
    14641 gtattgtacg ctgtgaacgc gttgggatct acctgtggaa aagtatggga ttaaaaaagc
    14701 cgatcagggc ttgatggtac gcatgatcgg gttttgcagg acgatcaatg tctcggtgga
    14761 ctgaatttca tcaattgttt ggatcttgtt gataagtaca tgctggagag cgtcgatcga
    14821 acggcacata acttttataa agatgctgta gtggccggtt gtgtaatagg cttcagtgac
    14881 ttcatcaagg ctttccagct ttgccagcgc ggaagggtag tctttggcgc tctttaatat
    14941 aatgccgata aagcagccta cgtcataacc gagctgcttc gggctgacat caatacgcgc
    15001 cccggtaatg atccccgcct gcttcatttt ctctactcga acgtgaatcg tccccggact
    15061 gacgccaaat tgtttcgcca gttcggcgta agcggtgcgc gcattgccca ttaatgcttc
    15121 caggatgcca cggtccagat tgtcgatcag ataattttcc ataggatttt cttatgcaga
    15181 ttgatgattc attctatttt agccttcttt tttaatgaat caaaagtgtg ttaggctttt
    15241 tattgaatga ttattgcatg tgtgtcggtt tttgttgctt aatcataagc aacaggacgc
    15301 aggagtataa aaaatgaaaa ccgcttacat tgccaaacaa cgccaaatta gcttcgtgaa
    15361 atctcacttt tctcgccaac tggaagaacg tctggggctg atcgaagtcc aggcgccgat
    15421 tcttagccgt gtgggggatg gcacgcagga taacttgtcg ggctgtgaaa aagcggtgca
    15481 ggtaaaagtg aaagctctgc ctgatgccca gttcgaagtg gttcattcac tggcgaagtg
    15541 gaaacgtcag accttagggc aacacgactt cagcgcgggc gaagggctgt acacgcacat
    15601 gaaagccctt cgccccgatg aagaccgtct ttctccgttg cactcggtct atgttgacca
    15661 gtgggactgg gaacgcgtaa tgggcgacgg tgagcgtcaa ttctcgactc tgaaaagcac
    15721 ggtagaggcg atctgggcgg gaattaaagc aaccgaagct gcggttaacg aagagtttgg
    15781 cctggcaccg ttcctgccgg atcagattca cttcgtacac agccaggagt tactgtctcg
    15841 ttatccggat ctcgatgcca aagggcgtga gcgggcgatt gcgaaagatc ttggcgcggt
    15901 attcctcgtc gggattggcg gcaagttgag tgatggtcat cgtcacgacg tgcgcgcacc
    15961 ggattatgat gactggagca ccccgtcaga gctgggccat gcgggcctga acggcgatat
    16021 tctggtgtgg aacccggtac tggaagatgc gtttgagctt tcttccatgg ggatccgcgt
    16081 ggatgccgac acgctgaagc atcagctggc gctgaccggt gatgaagatc gtctgcagct
    16141 ggaatggcat caggcgctgc tgcgcggtga aatgccgcag accatcggcg gcggtatcgg
    16201 ccagtctcgt ttgactatgc tgctgctgca actgccgcat atcggccagg ttcagtgtgg
    16261 agtatggcca gttgctgttc gcgagagcgt cccttctctg ctgtaataat ttatcgccgc
    16321 cagcgtctga gcaggcggct tcgcatcccg gtatcaaagc gccagatatg atcgaaaatg
    16381 cgcatgatgc cgggtttgcc gtgtgccgac atcgccacgg catgaaagcg atgctgatgt
    16441 acccgctgca gctctttcac tttactcgtc acgtcgtcag gcaaccgctg agcgataaaa
    16501 tcagaaatca ccaccgcatc ggcatcaaac cattccctgc tttgcaagcg ttccataatg
    16561 gcgcgaaaac aactggcaag atcggtgccg ccacgaaact gctggcttaa aaaacggatt
    16621 gcttgttcga tgccttgtgg gcctgaaagc tcataacgga cgatctcggt ggaaaatagc
    16681 ataatataac agcgccggtt ttcagcgaga gcaatgcgca tcaaagccag gcagaacgct
    16741 ttcgcacact gttcattaaa gccgcccatt gagccggaag tatccacaca gacaataaac
    16801 ggcccgcgcg gctgttcatc gtaatcttta tgtaccaccg gacgttcgag cactttttca
    16861 cgccacgact caccgtgcag gcgataggtg agcaactgtt tttccaccag ccgacggtaa
    16921 aactcatact ccagttccgt tatccctagt gtcgccagtt ctggcggcag gagacgtaaa
    16981 atatcatcgc tttgttgcag accatcaacc tgctcaggaa ccgtcgccgg ttcgcgcacc
    17041 atggtgcgga aggtttccat ctacgcatcg ttgcgcggta ttgatttggc ttcccgagaa
    17101 cgccccggct gctctgccag gcgtttcagt tccggctgtt cgttaagaaa ttcaccgtat
    17161 ttcacaataa actgatagtc gccacgttta agctgaccgg cgctcatatc ccacagacga
    17221 ccagctgcgg tattgttatc tgcgagaatc ggttcaagtt gtccgctcag cgtcatgcgt
    17281 tcctgaactt cacttaacag ttgttcgcgt tcttcttcta atagctgttg atttaacgtc
    17341 gttgcttgca cgatcagact taaacgccaa cgctggagaa aaagcgtgtg taacgctgaa
    17401 gttattgtgc tgttagcatc aaccaactgc cgggcttgtt ctgcccatgg ggaattcaga
    17461 cgatgcagta agtctaggat ctgtggtagc tgcacaataa actgcggcgt ggagaggagc
    17521 tggctttgct gatagcacat cacctcttcg gtgagttccg gcgggactcg ggcatctttc
    17581 agccgactgc gcagcgcctc acgccagcgg ggaacatcat cagtgattgc cgccttcagt
    17641 cgtgggaatt tttcaaagaa gaccgccagc tgcggtgagg ccagcagtgc gatgatcatc
    17701 tcttcgatca atccctcttc gctgacggcc agcatcacat taagcgtatc cagcgttagc
    17761 attgttgtgc ctggcgaatc tgtgcaccga catcctgtag gctggcttca atgcgaccta
    17821 accagtcgcc agggataaac agacattttt gctgttcgct gaataaagcg tgttgcttac
    17881 gccagtcgct ttcaagctct tccagttgtt gttttatttc acctggcaga ccttcagccg
    17941 atgaaccggg aagtgccagc gtactgcctt gtaaacttac atcgcgtaca acaagatgtt
    18001 gggtgctatc aacttccaga ttcagtttct gggcaaagcc gataccgttc agtttgccgc
    18061 gaatttcacc acctttactc agccactgtt ccagcgcgct acgctcaaag gagatatgaa
    18121 ccacttccat atcatgcagt tttaacggtt tttgcagcag cagagtcaga gtggaagcag
    18181 taacgttaac aggaagttga tactgctgac gacggctgaa aatgccgccc agacgaatta
    18241 acgttaaggc tgttttatcg ctttgttgct gctggagttg caggtgacgt tgcacaatcg
    18301 cgcccaggcg ggtcaacatc ccttgctgtt gccaggcatg accggtcatc aatacatcaa
    18361 tttgttgttg tatcaaattc aggctttgcg cgtcatacca caggcaatct ttcagcaaaa
    18421 tgagatcaac cggggcaaca gcactgcgac cgctaaaaaa ggcgctggcc tgcaataagc
    18481 ggatcgcttt tttccagcga cgatccgaga cataaggcgc atccggtaat ttatccagtt
    18541 gctggcgcag cataaaaatc agctcaaata catgatcggg cagcgtaatt tcaccaatct
    18601 ctttctgcca gcgttcatat tcttcatctg tgacctgcaa ggcatcagga accggattgt
    18661 cgttttcatc ctgttgactg gtcagcatgg agcggaaatt cgctttatct tgcactttat
    18721 ctaaccatag acgaatcagc atgcggtcat ataacgcttc cagactgctg tctgcttccg
    18781 gcagctcgtt ggaggccgcc accagtagac gcatcgggat tttttctacg tgtgcaccgt
    18841 tgcggaactg gcgctcgtta atggcggtga gcaaggtatt aagaattgcc gggcccgctt
    18901 tccagatctc atccagaaag acgatttctg cttccggcag gtaaccgctg gttaaacgtt
    18961 catagcgccc ttcatctttt agcgcctgaa tagaaagggg accaaaaact tcttccggcg
    19021 tggagaagcg ggtcatcaga tattcaaacg cgcgggcatt ctgaaaggcg aattttaagc
    19081 gccgggcgat caaacttttg gcaatacctg gcgggccaag gaggaacaca ctttcaccac
    19141 ttaatgccgc taataaacac aagcggatgg cgtggctacg ttcataaagc cccttttcca
    19201 acgaactgct caggcgggaa attctttccg ctaataaatg agggtgagcc ataatgaagt
    19261 ggcgtccttt cgtcaaaagt tctgcgtaaa ttgcgagtat agacgtttct tgctggtggc
    19321 taaaatagtc tcaaaggggg gt
//
LOCUS       DAJTNM010000075        19284 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTNM010000075 DAJTNM010000000
VERSION     DAJTNM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.075"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..1919
                     /gene="scpA"
                     /locus_tag="OO172_002644"
     CDS             <1..1919
                     /gene="scpA"
                     /locus_tag="OO172_002644"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5512778.1"
                     /translation="QYAGFSTAKESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDNPR
                     VAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAVLPVLAFYIVAAEEQGVTP
                     DKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISGYHMGE
                     AGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLFFGIGMDLFMNVAMLRAAR
                     YLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNVIRTTIEALAATLGGTQSL
                     HTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPLAGSYYIESLTDQIVKQAR
                     AIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGKRVIVGVNKYKLDHEDETD
                     VLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAAQHNENLLAAAVNAARVRA
                     TLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASEFDAIVAQTEQFLADNGRR
                     PRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTPEEIARLAVENDVHVAGAS
                     SLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAFLQERGVAAIYGPGTPMLD
                     SVRDVLNLISQHHD"
     gene            1912..2907
                     /gene="meaB"
                     /locus_tag="OO172_002645"
     CDS             1912..2907
                     /gene="meaB"
                     /locus_tag="OO172_002645"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5512779.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            2918..3703
                     /gene="scpB"
                     /locus_tag="OO172_002646"
     CDS             2918..3703
                     /gene="scpB"
                     /locus_tag="OO172_002646"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5512780.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            3727..5205
                     /gene="scpC"
                     /locus_tag="OO172_002647"
     CDS             3727..5205
                     /gene="scpC"
                     /locus_tag="OO172_002647"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR5512781.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(5147..6097)
                     /locus_tag="OO172_002648"
                     /pseudo
     CDS             complement(5147..6097)
                     /locus_tag="OO172_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6264..7004)
                     /locus_tag="OO172_002649"
     CDS             complement(6264..7004)
                     /locus_tag="OO172_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5512782.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(7097..7732)
                     /gene="argO"
                     /locus_tag="OO172_002650"
     CDS             complement(7097..7732)
                     /gene="argO"
                     /locus_tag="OO172_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5512783.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(7871..8731)
                     /gene="mscS"
                     /locus_tag="OO172_002651"
     CDS             complement(7871..8731)
                     /gene="mscS"
                     /locus_tag="OO172_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5512784.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(8922..10001)
                     /gene="fbaA"
                     /locus_tag="OO172_002652"
     CDS             complement(8922..10001)
                     /gene="fbaA"
                     /locus_tag="OO172_002652"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5512785.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(10216..11379)
                     /gene="pgk"
                     /locus_tag="OO172_002653"
     CDS             complement(10216..11379)
                     /gene="pgk"
                     /locus_tag="OO172_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5512786.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(11429..12448)
                     /gene="epd"
                     /locus_tag="OO172_002654"
     CDS             complement(11429..12448)
                     /gene="epd"
                     /locus_tag="OO172_002654"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5512787.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHSEAHIAAGAKKVLFSHPGSNDLGATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            12820..13251
                     /locus_tag="OO172_002655"
     CDS             12820..13251
                     /locus_tag="OO172_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5512788.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELEFSRQPCALSGYDELHISPVK"
     gene            13274..13852
                     /locus_tag="OO172_002656"
     CDS             13274..13852
                     /locus_tag="OO172_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5512789.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            13853..14161
                     /locus_tag="OO172_002657"
     CDS             13853..14161
                     /locus_tag="OO172_002657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512790.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            14177..14560
                     /locus_tag="OO172_002658"
     CDS             14177..14560
                     /locus_tag="OO172_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5512791.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNELAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            14548..15225
                     /locus_tag="OO172_002659"
     CDS             14548..15225
                     /locus_tag="OO172_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5512792.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTCSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            15219..15896
                     /locus_tag="OO172_002660"
     CDS             15219..15896
                     /locus_tag="OO172_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5512793.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(15868..16581)
                     /locus_tag="OO172_002661"
     CDS             complement(15868..16581)
                     /locus_tag="OO172_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5512794.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(16578..17087)
                     /gene="fumE"
                     /locus_tag="OO172_002662"
     CDS             complement(16578..17087)
                     /gene="fumE"
                     /locus_tag="OO172_002662"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5512795.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(17109..18074)
                     /gene="yggF"
                     /locus_tag="OO172_002663"
     CDS             complement(17109..18074)
                     /gene="yggF"
                     /locus_tag="OO172_002663"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5512796.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(18071..>19284)
                     /locus_tag="OO172_002664"
     CDS             complement(18071..>19284)
                     /locus_tag="OO172_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5512797.1"
                     /translation="EITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENHPVIT
                     GHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMIIPEI
                     AINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIALLA
                     CAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYVNTK
                     GMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNFKVP
                     FNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETVLNL
                     PDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKYLLA
                     QFGVDIGEAAQ"
BASE COUNT         4639 a         5182 c         4823 g         4640 t
ORIGIN      
        1 gtcagtatgc tggtttttca acagcaaaag agtccaacgc tttttatcgc cgtaacctgg
       61 ccgccgggca aaaaggtctt tccgttgcgt ttgaccttgc cacccaccgt ggctacgact
      121 ccgataaccc acgcgtggcg ggtgacgtcg gcaaagcggg cgtcgctatc gacaccgtgg
      181 aagatatgaa agtcctgttc gaccagatcc cgctgggtaa aatgtcggtt tcgatgacca
      241 tgaatggcgc agtgctacca gtactggcgt tttatatcgt cgccgcagaa gagcaaggtg
      301 ttacacctga taaactgacc ggcaccattc aaaacgatat tctcaaagag tacctctgcc
      361 gcaacaccta tatttaccca ccaaaaccgt caatgcgcat tatcgccgac atcatcgcct
      421 ggtgttccgg caacatgccg cgatttaata ccatcagtat cagcggttac cacatggggg
      481 aagcgggtgc caactgcgtg cagcaggtag catttacgct cgccgatggg attgagtaca
      541 tcaaagcagc aatctctgcc ggactgaaaa tggatgactt cgctcctcgc ctgtcgttat
      601 tcttcggtat cggcatggat ctgtttatga acgtcgccat gttgcgtgcg gcacgttatt
      661 tatggagcga agcggtcagt ggatttggcg cacaggatcc gaaatcactg gcgctgcgta
      721 cccactgcca gacctcaggc tggagcctga ctgaacagga tccgtataac aacgttatcc
      781 gcaccaccat tgaagcactg gctgcgacgc tgggcggtac tcagtcactg cataccaacg
      841 cctttgacga agcgcttggt ttgcctaccg atttctcagc acgcattgcc cgcaacaccc
      901 agatcatcat ccaggaagaa tcagaactct gccacaccgt cgatccactg gccggatcct
      961 attacattga gtcgctgacc gatcaaatcg tcaaacaagc cagagctatt atccaacaga
     1021 tcgacgaagc cggtggcatg gcgaaagcga tcgaagcagg tctgccaaaa cgaatgaccg
     1081 aagaggcctc agcgcgcgaa cagtcgctga tcgaccaggg caagcgtgtc atcgttggtg
     1141 tcaacaagta caaactggat cacgaagacg aaaccgatgt acttgagatc gacaacgtga
     1201 tggtgcgtaa cgagcaaatt gcttcgctgg aacgcattcg cgccgcccgt gatgatgccg
     1261 ccgtaaccgc cgcgttgaac gccctgactc acgccgcaca gcataacgaa aacctgctgg
     1321 ctgccgctgt taatgccgct cgcgttcgcg ccaccctggg tgaaatttcc gatgcgctgg
     1381 aagtcgcttt cgaccgttat ctggtgccaa gccagtgtgt taccggcgtg attgcgcaaa
     1441 gctatcatca gtctgagaaa tcggcctccg agttcgatgc cattgttgcg caaacggagc
     1501 agttccttgc cgacaatggt cgtcgcccgc gcattctgat cgctaagatg ggccaggatg
     1561 gacacgatcg cggcgcgaaa gtgatcgcca gcgcctattc cgatctcggt ttcgacgtag
     1621 atttaagccc gatgttctct acacctgaag agatcgcccg cctggccgta gaaaacgacg
     1681 ttcacgtagc gggcgcatcc tcactggctg ccggtcataa aacgctgatc ccggaactgg
     1741 tcgaagcgct gaaaaaatgg ggacgcgaag atatctgcgt ggtcgcgggt ggcgtcattc
     1801 cgccgcagga ttacgccttc ctgcaagagc gcggcgtggc ggcgatttat ggtccaggta
     1861 cacctatgct cgacagtgtg cgcgacgtac tgaatctgat aagccagcat catgattaat
     1921 gaagccacgc tggcagaaag tattcgccgc ttacgtcagg gtgagcgtgc cacgctcgcc
     1981 caagccatga cgctggtgga aagccgtcac ccacgtcatc aggcgctaag tacacagctg
     2041 cttgacgcta ttatgccgtt ctgtggtaac gccctgcgac tgggcgttac cggcacaccc
     2101 ggcgcgggga aaagtacctt tcttgaagcc tttggcatgt tgttgattcg tgagggatta
     2161 aaggttgctg ttattgcggt cgatcccagc agcccggtca ctggcggtag cattctcggg
     2221 gataaaaccc gcatgaatga cctggcgcgt gccgaggcgg cgtttattcg cccggtaccc
     2281 tcctccggtc atctgggcgg tgccagtcag cgagcacggg aattaatgct gttatgcgaa
     2341 gcggcgggtt atgacgtagt gattgtcgaa acggttggcg tcgcgcagtc ggaaacggaa
     2401 gtcgcccgca tggtggactg ttttatctcg ttgcaaattg ccggtggcgg cgatgatctg
     2461 cagggcatta aaaaagggct gatggaagtg gctgatctgc tcgttatcaa taaagacgat
     2521 ggcgataacc ataccaatgt cgccattgcc cggcatatgt acgagagtgc cctgcatatt
     2581 ctgcgacgta aatacgacga atggcagcca cggggtctga cttgtagcgc actggaaaaa
     2641 cgtggaatcg atgagatctg gcacgccatc atcgacttca aaaccgcgct aactgccagt
     2701 ggtcgtttac aacaagtgcg gcaacaacaa tcggtggaat ggctgcgtaa gcagaccgaa
     2761 gaagaagtac tgaatcacct gttcgcgaat gaagatttcg atcgctatta ccgccagacg
     2821 cttttagcgg tcaaaaacaa tacgctctca ccgcgcaccg gcctgcggca gctcagtgaa
     2881 tttatccaga cgcaatattt tgattaaagg aatttttatg tcttatcagt atgtgaacgt
     2941 tgccactatc aacaaagtgg cggtcattga gtttaactat ggacgaaaac ttaatgcctt
     3001 aagtaaagtc tttattgatg atcttatgca ggcgttaagc gatctcaacc ggccggaaat
     3061 tcgctgtatc attttgcggg cgccaagtgg atccaaagtc ttctccgcag gtcacgatat
     3121 tcacgaactg ccgtctggcg gtcgcgatcc gctctcctat gatgatccat tgcgtcaaat
     3181 cacccgcatg atccaaaaat tcccgaaacc gatcatttcg atggtggaag gtagtgtttg
     3241 gggtggcgca tttgaaatga tcatgagttc cgatctgatc atcgccgcca gtacctcaac
     3301 cttctcaatg acgcctgtaa acctcggcgt cccgtataac ctggtcggca ttcacaacct
     3361 gacccgcgac gcgggcttcc acattgtcaa agagctgatt tttaccgctt cgccaatcac
     3421 cgcccagcgc gcgctggctg tcggcatcct caaccatgtt gtggaagtgg aagaactgga
     3481 agatttcacc ttacaaatgg cgcaccacat ctctgagaaa gcgccgttag ccattgccgt
     3541 tatcaaagaa gagctgcgtg tactgggcga agcacacacc atgaactccg atgaatttga
     3601 acgtattcag gggatgcgcc gcgcggttta tgacagcgaa gattaccagg aagggatgaa
     3661 cgctttcctc gaaaaacgta aacctaattt cgttggtcat taatccctgc gaacgaagga
     3721 gtaaaaatgg aaactcagtg gacaaggatg accgccgatg aagcggcaga aattatccag
     3781 cataacgaca tggtggcatt tagcggcttt accccagcgg gttcgccgaa agccctaccc
     3841 gccgcgattg cccgcagagc taacgaacag catgaggcca aaaagccgta tcaaattcgc
     3901 ctgctgacgg gggcgtcaat cagcgccgcc gctgacgatg tactttctga cgccgatgct
     3961 gtttcctggc gtgcgccata tcaaacatcg tccagtttac gtaaaaagat caatcagggc
     4021 gcggtgagtt tcgttgacct acatttgagc gaagtggcgc aaatggtcaa ttacggtttc
     4081 ttcggcgaca ttgatgttgc cgtcattgaa gcatcggcac tggcaccgga tggtcgagtc
     4141 tggttaacca gcgggatcgg taatgcgccg acctggctgc tgcgagcgaa gaaagtgatc
     4201 attgaactta atcactatca cgatccgcgc gttgcagaac tggcagatat tgtgattcct
     4261 ggcgcgccac cgcggcgcaa tagcgtgtcg atcttccatg caatggatcg cgtcggtacc
     4321 cgctatgtgc aaatcgatcc gaaaaagatt gtcgccgtcg tggaaaccaa cttgcccgac
     4381 gccggtaata tgctggataa gcaaaatccc atgtgccagc agattgccga taacgtggtc
     4441 acgttcttat tgcaggaaat ggcgcatggg cgtattccgc cggaatttct gccgctgcaa
     4501 agtggcgtgg gcaatatcaa taatgcggta atggcgcgtc tgggggaaaa cccggaaatt
     4561 cctccgttca tgatgtattc ggaagtgcta caggaatcgg tggtgcattt actggaaacc
     4621 ggcaaaatca gcggggccag cgcctccagc ctgacaatct cggccgattc cctgcgcaag
     4681 atttacgaca atatggatta ctttgccagc cgcattgtgt tgcgtccgca ggagatttcc
     4741 aataacccgg aaatcatccg tcgtctgggc gtcatcgctc tgaacgtcgg cctggagttt
     4801 gatatttacg ggcatgcaaa ttcaacgcac gtagccgggg tcgatctgat gaacggcatc
     4861 ggcggcagcg gtgattttga acgcaacgcg tatctgtcga tctttatggc tccgtcgatt
     4921 gctaaagaag gcaagatctc aaccgtcgtg ccaatgtgca gccatgttga tcacagcgaa
     4981 cacagcgtca aagtgatcat caccgaacaa gggatcgccg atctgcgcgg tctttccccg
     5041 cttcaacgcg cccgcactat cattgataat tgtgcacatc ctatgtatcg ggattatctg
     5101 catcgctatc tggaaaatgc gcccggcgga catattcacc acgatcttag ccacgtcttc
     5161 gacttacacc gtaatttaat tgcaaccggc tcgatgctgg gttaatggtt tgtcccgtca
     5221 cgatcttcag caatatgctg aagatcgtgg cggtataacg atgatttctg attgaataaa
     5281 gatattcctg catatacatc ggcctgtgcg gcgcatcaaa atatttcagc gttgccggat
     5341 tcaccagtca ccaggcaaaa tgcgggatca ccgtcagaaa atgtccgcgc tccacggcgc
     5401 tgattttggc cataaaactg tagggacgat aaataatggt gggattgatc ccacaaggtc
     5461 gcatattcgc atcaatcatt gcttcgaagt tagcccggtt ttgaaagcgc atttgtagcc
     5521 aggggagctc ctgaagcagc tcttgctctt gacgctcttt ataacggcga gaaacgagaa
     5581 agcccaatca cagcggtggt aattcactga tagtcaggtt ttccagttcc tgaactcgtg
     5641 cagatacatg ctgtggggag ataataaaat ctaactgacg gtcaaaaaga ttatcgataa
     5701 cgccgttttc gctgaactca actggctggg cggtcacgcc ttcatactta tctccgagac
     5761 taatcaactg atcgaaaata attgtcggat aagtattatc tatgccaatc acaatttctc
     5821 gtgaacgacg ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg
     5881 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
     5941 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
     6001 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
     6061 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
     6121 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
     6181 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
     6241 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
     6301 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
     6361 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
     6421 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
     6481 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
     6541 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
     6601 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
     6661 accgttctca cagctcggta gcctttcagg atacttttac catcctgata gtcataatct
     6721 ggctgggtgc gtaaattcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
     6781 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
     6841 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     6901 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     6961 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     7021 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     7081 ttccatcaga tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     7141 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg tgctgtgatt
     7201 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
     7261 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     7321 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     7381 ctgccaacat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     7441 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccag agcaagaaga
     7501 ctacgccgcc ccaagtgacc agcgctagca accacggcga ctgcatcaat aacgcgctac
     7561 caccaaaaat ccccgcgcaa atcaggacca aatcgctgat agcgcaaagt aaggcaatca
     7621 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt gggccgagcg
     7681 gcagaatcat agctgcccca agagcaagac cttgaaagta ataagaaaac acgttggata
     7741 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     7801 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     7861 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     7921 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     7981 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     8041 atgcaccaag ttcgttcagg cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     8101 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     8161 caataataaa ttcgttacgg cgaactggct cgcgggagaa gttaataata tttccggcaa
     8221 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     8281 aaatctgcac actcagcaca gtaccggcta cgccgccgag gtcaacatat tctccggcac
     8341 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca
     8401 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
     8461 cacccacgcg tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     8521 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     8581 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     8641 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     8701 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     8761 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     8821 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaaa
     8881 gacccgcaga gcgggccttg agataagcag aaaggaatga cttacagaac gtcgatcgcg
     8941 ttcagttcct ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc
     9001 catacgcgcg gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccagc
     9061 tgaccctgca gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc
     9121 cattgggtat cggtatcgat gttcattttc actacgccgt agcttacgga gtctttgatt
     9181 tcctgagcag tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg
     9241 ttgtgtttct tagaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg
     9301 ttacccggct tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg
     9361 ctgattttgc tcagctcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca
     9421 gaagcgtcca tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg
     9481 atttccagag tcatgccgat tttggacata cgctccaggt atttagagca gatttcgatg
     9541 ttctcttgca gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg
     9601 gtagctgcga agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc
     9661 ttcgcgcagt ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga
     9721 acgtgatgcg caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc
     9781 acgcctttac cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct
     9841 ttaactttag cagcagtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact
     9901 gctggcagtg cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca
     9961 gtgattacgc caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt
    10021 cgggccttat actcataata cttcaagttg catgtgctgc gtctgcgttc gctcacccca
    10081 gtcacttact tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa
    10141 ttatttagag tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc
    10201 ctgccctgtg attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac
    10261 tttaccttcc acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc
    10321 agcaatgccg aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc
    10381 ttcgctgtct gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc
    10441 gaacacgcca accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag
    10501 ttcctgagcg gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac
    10561 agatttcagg gtagccggtg cagtttcaga gaactcggtt gctacgcgaa catcagacgg
    10621 aaccgggatg ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta
    10681 cagggattta cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc
    10741 aacaatcagc tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac
    10801 tttagaacca ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag
    10861 cgcgtccagt tcagctgcca gcagcgggcc tgcgcacgca acgtcagcga atttaccgat
    10921 accgtgagta gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc
    10981 acacagtgca gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa
    11041 gcgaacgttt tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc
    11101 tttaaccaga cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag
    11161 agagaattct tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac
    11221 ttttgcgcct tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc
    11281 gctggttact ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt
    11341 cccagcaaga tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc
    11401 taaaagtttt gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg
    11461 tcgtgtcgag cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga
    11521 tcaggtgtgc gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt
    11581 ggttaaaatc tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac
    11641 cttgtgctgc tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg
    11701 tcacgcttaa atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa
    11761 agcgatcgtt aaattgcgga aaaaatcgtg tgataccagc ggccagttta gtatcgaccg
    11821 gaatgatcga ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa
    11881 cctgttgatc gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg
    11941 catcatctaa caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa
    12001 cgatgcggtg ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgccga
    12061 gatcgttact gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt
    12121 cgctatgctc gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt
    12181 cacgccaggg gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa
    12241 caaaaagttg atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat
    12301 atttcaacaa atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa
    12361 tttccgcccg gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga
    12421 agccatttat cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt
    12481 tgccagacag agtaatctag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac
    12541 tgattggtta attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac
    12601 gtggaataaa aggaatgatt gtccagtcaa agaaagcaat tatctgactt acgtcacgtt
    12661 ttcagccccg cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa
    12721 atcttttcgt tgtgccgtca caaaccctga cttatatcag aatatggctg atgcagcgtc
    12781 gatattatcc gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca
    12841 tattcttcct ccgcacgctt ttacgcactg cgcctcctcc ccgggcagga agttttttcc
    12901 cagctacacg cttttgcaca gcaacatcaa cttcatgccg cctggattgc aggctgtacc
    12961 ggtagcctga ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctcctgaac
    13021 ggtacattcg aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat
    13081 ctctgcgttt ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc
    13141 gtgcggacca cgcttgaact ggtgatcggt agccttgagg aactggagtt tagccgccaa
    13201 ccttgcgcgc tttctggtta cgacgagttg catatttccc ccgttaaata actactttca
    13261 aagaggtttt cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat
    13321 atctatcgcc atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt
    13381 tctcgactcc atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct
    13441 gaccggctta ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt
    13501 tgcacccgtc gcgatggtca tcggcctggt ctccggttgg ctggcgcgag cgggttggtt
    13561 tcgcaccctg ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt
    13621 cgctgtaccg ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt
    13681 cgttgcctgg atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacggtgat
    13741 aggcgcgaac ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca
    13801 gctgccgatt cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc
    13861 atttacctca ttaacactgt gggcattagc ggcctgcacc acgcttatcc tgcccgcgca
    13921 gaccattttg ccgatctata gcgcggcgac ctttttctgc cttattgcgc taaaggccac
    13981 gcggcggcgg gcaaaatatg tcgtctggct gatgttctcg ctgggagccg ggctgtggct
    14041 ggtacacggt ggctggctac cagaatggct aagcggcacg ccacgctcgc cagaacggtg
    14101 gtcgcatgct attacgctct ggttgctgat tctggcgatt gtttcaacct cacagttgtg
    14161 aatgcagtac gtccccgtgc aatgttttat tcgcgctctg tttgcttctc gcctgccgcc
    14221 aggcgtcgct tatctgtttg ccgggccgtt attggtcgtt gagcagttaa aacggcaatt
    14281 agcgattatt catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca
    14341 gcgtttacgt gctatgcccg cgttaattat tccgctaaca cacaatgcgc ttaacgagct
    14401 ggctgtccgt ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac
    14461 gttgtgggca ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct
    14521 gatgctgaca gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg
    14581 acacactcaa cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc
    14641 gccatctttg gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg
    14701 tatccggact ttctgccaag agagataaca ggcacgggga cgctcctcgg tacgcccatt
    14761 gggcatctcc cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat
    14821 ttgcagctct ccggctgtac ctgtagcgtg gaagaagaag ttgcctttgg gccagaaaat
    14881 ctgtgtcttg ctgaagcaga gattatggcg cggattgatg ccgccctgac tctgaccgag
    14941 tgccagccgt tgcgccatcg tcacccggca acactctccg gtggagagac acagcgcgtg
    15001 gtcatcgcct gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagc
    15061 cgcctgacgc cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgtttgaa
    15121 cgtggttcgc tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag
    15181 caagcgtggc aattgcaaaa cggagcgctc cagccactat gttaacgtta aataagatat
    15241 cttatcgctg gcctggcgcg gcaacggatt gtctttgcga catttctctg caactcaaac
    15301 aaggtgaatg gttggcgctg accggtgaca atggcgcagg gaaatcgacg ctgttacgag
    15361 taatggcagg tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga
    15421 aaaacctgaa aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa
    15481 accaactttt tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat
    15541 gcccggcgga tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag
    15601 acactgccaa ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg
    15661 cctgcctgga agcactctct ccgccactat tactgctgga cgagccgagt cgggattttg
    15721 acgaaaattg gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacga
    15781 gcgtggtagc aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc
    15841 gactggaaga tggtgtaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt
    15901 catcattaag gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc
    15961 gtcggtacgg tcataaaagg cttctgcatc tgccagcgat acccctcccg ccagcttgcg
    16021 ccccaccaga cgctcccgca acgcagcagc tggcgcgttg ataaagatcg agaaatcaca
    16081 aaattgcgca agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat
    16141 gacgagtggt gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcgta
    16201 ctgcggccac gtacaatccc cttccaccac ctggcgcaga ttttccgcca gtttcgcaac
    16261 gtcaaatgtc tctggtgcgc ctttgaaggg gcgcaattga tgcgcgtcca gccagctatt
    16321 gtaatggtga aaaccatcca tcgggagcgt ctgaatagcg ggcagctccg ggtcttgttg
    16381 cgcgagatat tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag
    16441 aaaaaccacc gtacgccgct gcggatttac cgtctgtaag gccgccagca tacgcagcaa
    16501 cggtttatgc acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt
    16561 caccgttaat tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc
    16621 agcgccgttt tgaccattcc gctaaaacgc gcctctgacc agacggcgaa atcggcgaac
    16681 ttcatcgctt ttaacgcatc gtagagcgtc gcattgcgag ttatactgtt gacattgctg
    16741 ataaaatccc aggttatatc atcggcaaat ccgggcgttt catcctgttc gttcaggtaa
    16801 tgccagtact gggaaaagtg cgtgatatcg gcatacagcc atttatccat tttccccagc
    16861 gcataaatca aacgcagcgc aacatcaata tcgtcaagcg ggccgctttg ggcgagtaac
    16921 ggcttcactg catattcgac gatctcttca tcgttatcga caaacagcga cggcagaaac
    16981 tggcgtatcc cctggagcaa aatagaatgc gcagttttca taaatgaaaa taaattgtcc
    17041 tgtgcatcca gttgctcaag cacatcatct tctgtcagag tcgccattgg tcttccgata
    17101 gatcaccatc aatgcaggga gtctattata ttacacgttt ggtccgcgcc gccgatcagt
    17161 aatgtctgcg tccgcacccc attcgccgtc tgttggatac cgttcaccaa ctcgccgccc
    17221 gtcacgcccg tagcgctaaa gaggatatcg ttccccctca ccagttcatc gagcgagtac
    17281 acacggttaa cgtcaacgcc cattgcctta caacgcttgc gctcctgctc tgcaatctgc
    17341 cgattttccg tgtaatcccc tttagcctga caaaagtcaa tcagctcggc ctgcatatcc
    17401 ccgcctaacg ctttaacggc acaggcagaa atcacgcctt ctggcgcacc gccgatggtg
    17461 tacatcacat cgtaaggatt atcctgccag caggtcagca cgctggcggc gacatcgcca
    17521 tccggcaggg caaaaacctt cacgcccagc tgtgttgctt cttcaatcgc ggcactcaga
    17581 cgcggtttat ccagcgtaac catgcgcagc ttgtccagcg gtttacctaa cgcccgcgcc
    17641 acgttacgca agttgtccgc cagaggcaaa gacagatcaa tcgccccggc agccagtcga
    17701 ttaacaacca gctttttcat atacatatcg ggcgcatgta acaggctatc tcgaggagcg
    17761 aaagccatga ccgccagcgc attgctctgc cccatcgcca ccatccgcgt gccttcaatg
    17821 ggatcaaccg cgatatcgac ttctggccca tcgcctttgc ccacctcttc gccaatccac
    17881 agcatcggcg catggtcaat ttctccttcg cagataacca ctcgtccacg aaaagcgacg
    17941 tcgtttaatg cctggcgcat cgcggtgacc gccaggccat caattttatt tttgtcgcca
    18001 catccggttt gcggccaggc agccaacgcg gcctgttccg taacgcgaaa taatggccac
    18061 gccagggaca tcattgcgcg gcctccccga tatcaacgcc aaattgtgcc agcagatatt
    18121 tctcggcctg ttcattccag atgccgtgcg tttcctcaac cagccgcgcc aactctttaa
    18181 acagcggatc ggttttgcct ttttcggcaa aatcggcaat ggcagtgagc ggcatggtca
    18241 cgccgttata aatgagtttt ttaccgccag ggatatccgg cagattgagc acggtttctg
    18301 gcaccgcatc caggccacca atatgggtca ccataaacga cggctgtaac tgtcctgtgg
    18361 cgctaagggc aatcgcctct ttcatgtcgt ccgttgaacc gccggatgta ccgactacgt
    18421 gcgtgctgtt gtaatggacg ttgtagaaat taaacggcac tttgaagttt ttatccgtca
    18481 gcccggcaaa gaagttcaga cagccatctt ccgccagtaa ttcgtcagcc atctcaacga
    18541 cagcaggcac cgccgcataa acaaaaatgt catcgaaccc gacatctcct gtcagcgccc
    18601 gcagcatctg gataggatcg ctcatccctt tggtattcac atacaccagc tcaatgcctt
    18661 tactggccgc cagatccacc ggcagcagct tctgcacctg cgccaggcgt ttgtcgtcga
    18721 tatcgaccac caccacccgt gacggttgta tgccaccgtt aatggcgtaa tcgatagcgc
    18781 caatgcccat cggacccgca cacgccagca gtgcaatatt gccgccaggt ttgacgccca
    18841 tgcgatgctc ataaacatat tgcgtggtgt gataattggc atgataagca ccaataatgc
    18901 agcacatagg ctctgccagc gacgccgcag caaaataaga gccgtgatac ggtaatacgc
    18961 agcccaaatt aatggcgatt tcgggaataa tcatataagt ggcattgccg ccaaaatatt
    19021 cgtagctgta gcccgctgaa tatccgcttg gtaaccccat cgccggttgc aatacaaaac
    19081 gctggccttt tttatatttg ccagtgagat ttttacccac ttcgacaata atcccggcac
    19141 attcatgccc ggtaatgacc ggatgatttt ctaaatcgtc gggtacgcgt ttatgttcac
    19201 taccgagtaa cgccgctttc caggtcgata aacagacgct gtcagaaatt acactcacta
    19261 acagttcatt atcggtaatt tctg
//
LOCUS       DAJTNM010000076        18260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTNM010000076 DAJTNM010000000
VERSION     DAJTNM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.076"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO172_002665"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO172_002665"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5512798.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO172_002666"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO172_002666"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5512799.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2999)
                     /locus_tag="OO172_002667"
     CDS             complement(1728..2999)
                     /locus_tag="OO172_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5512800.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3182..4386)
                     /locus_tag="OO172_002668"
                     /pseudo
     CDS             complement(3182..4386)
                     /locus_tag="OO172_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4486..4842)
                     /locus_tag="OO172_002669"
                     /pseudo
     CDS             complement(4486..4842)
                     /locus_tag="OO172_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4978..5085)
                     /gene="rprA"
                     /locus_tag="OO172_002670"
     ncRNA           complement(4978..5085)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO172_002670"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="OO172_002671"
     CDS             complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="OO172_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5512801.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAILAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6772..9828
                     /locus_tag="OO172_002672"
     CDS             6772..9828
                     /locus_tag="OO172_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5512802.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVVLIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9825..10235
                     /gene="menI"
                     /locus_tag="OO172_002673"
     CDS             9825..10235
                     /gene="menI"
                     /locus_tag="OO172_002673"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5512803.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10335..10523
                     /locus_tag="OO172_002674"
     CDS             10335..10523
                     /locus_tag="OO172_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5512804.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11071..11439
                     /gene="sufA"
                     /locus_tag="OO172_002675"
     CDS             11071..11439
                     /gene="sufA"
                     /locus_tag="OO172_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5512805.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11448..12935
                     /gene="sufB"
                     /locus_tag="OO172_002676"
     CDS             11448..12935
                     /gene="sufB"
                     /locus_tag="OO172_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5512806.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12945..13691
                     /gene="sufC"
                     /locus_tag="OO172_002677"
     CDS             12945..13691
                     /gene="sufC"
                     /locus_tag="OO172_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5512807.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13666..14937
                     /gene="sufD"
                     /locus_tag="OO172_002678"
     CDS             13666..14937
                     /gene="sufD"
                     /locus_tag="OO172_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5512808.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14934..16154
                     /gene="sufS"
                     /locus_tag="OO172_002679"
     CDS             14934..16154
                     /gene="sufS"
                     /locus_tag="OO172_002679"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5512809.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQAQGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16167..16583
                     /gene="sufE"
                     /locus_tag="OO172_002680"
     CDS             16167..16583
                     /gene="sufE"
                     /locus_tag="OO172_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5512810.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16732..17736
                     /gene="ldtE"
                     /locus_tag="OO172_002681"
     CDS             16732..17736
                     /gene="ldtE"
                     /locus_tag="OO172_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5512811.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OO172_002682"
     CDS             complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OO172_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5512812.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4807 a         4403 c         4689 g         4361 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccagccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcttgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtaaccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaacggt ttgctgttca tttgcggtaa ttcattagct
     2401 acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt
     2461 acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata
     2521 tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg
     2581 accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc
     2641 agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg
     2701 caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc
     2761 cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa
     2821 ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgccaga
     2881 gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa
     2941 atacaaagaa cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt
     3001 gttctccgac ggtttacata attactatac tgaagatgat tttccatggg tacatggcat
     3061 aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt
     3121 agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc
     3181 attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc
     3241 gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg
     3301 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
     3361 ccgcactgta atagatccct gtagctttac ctttagcgta agggaaacgt tcagctatta
     3421 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
     3481 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
     3541 aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa
     3601 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
     3661 atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg
     3721 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
     3781 gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
     3841 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
     3901 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
     3961 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
     4021 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
     4081 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgccaaaa aagaaggcca
     4141 tatagcagca cattccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
     4201 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
     4261 ccgcggcatt agtctgacaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
     4321 cgcccatacc atgcaccagg taataaaata caacccaagt gcggtagggt aatagggatt
     4381 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4441 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4501 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4561 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4621 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttactgacg
     4681 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4741 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4801 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4861 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4921 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4981 aaaaaaagcc catcgtggga gatgggcaaa gactacacac agcaattcgt tgtttcactc
     5041 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     5101 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5161 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     5221 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5281 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggcggcggg
     5341 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5401 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5461 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5521 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5581 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5641 attaacaccg ttagcagcgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5701 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgag gatagcctgt
     5761 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5821 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5881 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca agtggtggtg
     5941 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     6001 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     6061 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     6121 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     6181 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6241 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6301 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6361 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6421 cgcgacgtgg acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6481 aggccgattc tgtccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6541 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6601 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6661 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6721 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6781 cagatttccc aggcacccgg cgtcgttcaa ctggtgctta attttttgca agagctggag
     6841 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6901 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6961 gtggtgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     7021 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     7081 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     7141 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     7201 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7261 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7321 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7381 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7441 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7501 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7561 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7621 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7681 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7741 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7801 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7861 cgagttaatg cactctgtgt gcggcttgat gagctgattg ctagccaaca ggcaggtgtg
     7921 attggctggc aggtgtgccg cgagctggcg ggggttgaac gtatctatgc gatgcgcaaa
     7981 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     8041 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     8101 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     8161 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     8221 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8281 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8341 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8401 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8461 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8521 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8581 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8641 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8701 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8761 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8821 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8881 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8941 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     9001 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     9061 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     9121 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     9181 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9241 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9301 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9361 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9421 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9481 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9541 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9601 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9661 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9721 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9781 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9841 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9901 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9961 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
    10021 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    10081 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    10141 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    10201 gctgttcgtc acgattgacg accgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10261 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10321 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10381 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10441 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10501 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10561 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10621 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10681 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10741 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10801 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10861 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10921 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag catgttttac
    10981 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    11041 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    11101 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    11161 ctggtggcaa agcagccagg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    11221 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11281 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11341 gaaatcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11401 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11461 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11521 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11581 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11641 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11701 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11761 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11821 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11881 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11941 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    12001 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    12061 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    12121 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    12181 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12241 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12301 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12361 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12421 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12481 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12541 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12601 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12661 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12721 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12781 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12841 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12901 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12961 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    13021 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtact ttatcggcaa
    13081 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    13141 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    13201 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13261 tgcgcagcta tcgcggccag gaaacgcacg accgctttga ttttcaggat ttgatggaag
    13321 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13381 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13441 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13501 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13561 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13621 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13681 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13741 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13801 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13861 gcgggagata tatccccaca gcagcgtgat gccttagcgt taacgttaga cgccgtgcgg
    13921 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13981 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    14041 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    14101 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    14161 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    14221 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14281 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14341 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14401 ttcctgctgg gtggcgcagt gttacgacac aacaccagta cgcaactcaa tggcgaaaac
    14461 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14521 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14581 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14641 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14701 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14761 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14821 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14881 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14941 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    15001 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    15061 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    15121 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    15181 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15241 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15301 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15361 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15421 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15481 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15541 aggcggtgat gcatcatccg gtggatgttc aggcgcaggg ttgcgacttt tacgtgttct
    15601 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15661 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15721 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15781 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15841 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15901 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15961 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    16021 actgcgctat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    16081 ccatgtataa cacccatgaa gaagtggatc gtctggtgac cggcctgcaa cgtattcacc
    16141 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    16201 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16261 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16321 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16381 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16441 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16501 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16561 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16621 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16681 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16741 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16801 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16861 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcggcaatg
    16921 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16981 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    17041 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    17101 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    17161 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    17221 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    17281 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17341 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17401 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17461 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17521 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17581 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17641 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17701 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    17941 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    18001 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    18061 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    18121 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    18181 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt
    18241 atatagtgaa atccgatttg
//
LOCUS       DAJTNM010000077        17458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTNM010000077 DAJTNM010000000
VERSION     DAJTNM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.077"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            564..1334
                     /locus_tag="OO172_002683"
     CDS             564..1334
                     /locus_tag="OO172_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5512813.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO172_002684"
     CDS             1325..1798
                     /locus_tag="OO172_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5512814.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO172_002685"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO172_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5512815.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPVSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO172_002686"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO172_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5512816.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO172_002687"
     CDS             3233..3727
                     /locus_tag="OO172_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5512817.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="OO172_002688"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="OO172_002688"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5512818.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO172_002689"
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO172_002689"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR5512819.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8485
                     /locus_tag="OO172_002690"
     CDS             7571..8485
                     /locus_tag="OO172_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5512820.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGILTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8699..10060
                     /locus_tag="OO172_002691"
     CDS             8699..10060
                     /locus_tag="OO172_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5512821.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="OO172_002692"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="OO172_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5512822.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="OO172_002693"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="OO172_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5512823.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="OO172_002694"
     CDS             12610..13899
                     /locus_tag="OO172_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5512824.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPQVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="OO172_002695"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="OO172_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5512825.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVDDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLHQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="OO172_002696"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="OO172_002696"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5512826.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="OO172_002697"
     CDS             16225..16605
                     /locus_tag="OO172_002697"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5512827.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17458
                     /gene="hpaH"
                     /locus_tag="OO172_002698"
     CDS             16673..>17458
                     /gene="hpaH"
                     /locus_tag="OO172_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5512828.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGHPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSI"
BASE COUNT         4294 a         4627 c         4374 g         4163 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccggtaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcat
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agttcggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg caggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtggacg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcac cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgagct gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcat ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccatt
//
LOCUS       DAJTNM010000078        17226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTNM010000078 DAJTNM010000000
VERSION     DAJTNM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.078"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="OO172_002699"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="OO172_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5512829.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3172)
                     /gene="hemX"
                     /locus_tag="OO172_002700"
     CDS             complement(1967..3172)
                     /gene="hemX"
                     /locus_tag="OO172_002700"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5512830.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3194..3934)
                     /gene="hemD"
                     /locus_tag="OO172_002701"
     CDS             complement(3194..3934)
                     /gene="hemD"
                     /locus_tag="OO172_002701"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5512831.1"
                     /translation="MSILVTRPSPAGEELVRRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3931..4872)
                     /gene="hemC"
                     /locus_tag="OO172_002702"
     CDS             complement(3931..4872)
                     /gene="hemC"
                     /locus_tag="OO172_002702"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5512832.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5259..7805
                     /gene="cyaA"
                     /locus_tag="OO172_002703"
     CDS             5259..7805
                     /gene="cyaA"
                     /locus_tag="OO172_002703"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5512833.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7845..8165)
                     /gene="cyaY"
                     /locus_tag="OO172_002704"
     CDS             complement(7845..8165)
                     /gene="cyaY"
                     /locus_tag="OO172_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5512834.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8213..9526)
                     /locus_tag="OO172_002705"
     CDS             complement(8213..9526)
                     /locus_tag="OO172_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5512835.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9523..11001)
                     /locus_tag="OO172_002706"
                     /pseudo
     CDS             complement(9523..11001)
                     /locus_tag="OO172_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            11190..11393
                     /locus_tag="OO172_002707"
     CDS             11190..11393
                     /locus_tag="OO172_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5512836.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11430..12254
                     /gene="dapF"
                     /locus_tag="OO172_002708"
     CDS             11430..12254
                     /gene="dapF"
                     /locus_tag="OO172_002708"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5512837.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12251..12958
                     /locus_tag="OO172_002709"
     CDS             12251..12958
                     /locus_tag="OO172_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5512838.1"
                     /translation="MKQLGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12955..13851
                     /gene="xerC"
                     /locus_tag="OO172_002710"
     CDS             12955..13851
                     /gene="xerC"
                     /locus_tag="OO172_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5512839.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13851..14567
                     /gene="yigB"
                     /locus_tag="OO172_002711"
     CDS             13851..14567
                     /gene="yigB"
                     /locus_tag="OO172_002711"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5512840.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14651..16813
                     /gene="uvrD"
                     /locus_tag="OO172_002712"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14651..16813
                     /gene="uvrD"
                     /locus_tag="OO172_002712"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5512841.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16960..>17226)
                     /locus_tag="OO172_002713"
     CDS             complement(16960..>17226)
                     /locus_tag="OO172_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512842.1"
                     /translation="VINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACY
                     AKAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         4007 a         4430 c         4576 g         4213 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatctacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtttagc ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct
     2101 gcatcagttt ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat
     2161 tttgctggct taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat
     2221 cgtagtaagc acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct
     2281 gatgacgcgg tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat
     2341 agatatcctg atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa
     2401 tgaagttgtc cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg
     2461 aactggaaag ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca
     2521 gacgcaggtt atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat
     2581 ccacctgcga tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa
     2641 tcagactcgg gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag
     2701 aggtcgtgac gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg
     2761 cctgagccag cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga
     2821 cttcatccaa ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag
     2881 cttgttgctt aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg
     2941 ccgtcagttg gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac
     3001 cccagccata caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa
     3061 tcaatgcagt attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca
     3121 cggcctccct ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt
     3181 cctgttatga gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga
     3241 ccttaatgtc ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca
     3301 atagtcgaca gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt
     3361 gctgcaacat ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca
     3421 tcgcttcttc tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga
     3481 cctcagcacc gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta
     3541 atatcagcgc acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc
     3601 tgatttcccg atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg
     3661 cggtggtgcg tccaatggtg aaataagtag gtagttgggg ccatttacga tcttgctgat
     3721 gcagctgtga ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct
     3781 cccccagcgc tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa
     3841 tcagcggaaa atgccaggcc acctgcccca gtgtgcgcag acgtctcact aactcttctc
     3901 cagcgggaga cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc
     3961 agcgaggatc tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg
     4021 ttcggcatct tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc
     4081 gccgaccagc gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg
     4141 cacctgacat ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc
     4201 agtttcgtgg tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca
     4261 ttcaataccg actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc
     4321 gcgaatgcgg gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc
     4381 gtattcgccg ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat
     4441 aatcagatcc gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac
     4501 gatactgcct gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg
     4561 aggatcttca cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac
     4621 atctttcatt gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc
     4681 ttttacaaat aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc
     4741 gcgcgtcacc atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc
     4801 tttgacatag tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac
     4861 attgtctaac atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca
     4921 gagtgatgtc agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg
     4981 gcagtggatc ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg
     5041 gtgctacact tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga
     5101 tcacgtttta gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa
     5161 cggttacctt tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga
     5221 atcacagtca tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc
     5281 tgaaacagag actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg
     5341 ggcctgcatt ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc
     5401 taatgccggg ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg
     5461 aaactcaacg ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc
     5521 caccgaaagg tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag
     5581 ggcaaagttg ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg
     5641 aagagcgcca gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg
     5701 gtgtggaagt cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca
     5761 gcctgggggg cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc
     5821 gtactgccgt gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag
     5881 aagagcatta cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg
     5941 aatggctgga tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc
     6001 tttggcagct ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc
     6061 tggaagccta ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc
     6121 gtttgcacga cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc
     6181 gtgttactga atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct
     6241 gcttctattt aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc
     6301 gcgcagtgtt gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc
     6361 tcgataaccg tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg
     6421 acgcgatgat gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg
     6481 tctccgccag tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag
     6541 cattgccagg taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga
     6601 atctgacctt tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata
     6661 accgcgcgcc aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc
     6721 tgaataaact ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt
     6781 atattaaagg taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt
     6841 cgcaccattt cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg
     6901 agattcgcca tctggcgatt atcgttaacc tggaatatga cccgacagct gcgttccgca
     6961 atcaggtggt gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa
     7021 attgcctggt aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc
     7081 tgcacttcaa cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc
     7141 atcaggacgc cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg
     7201 gcttaatccg tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca
     7261 gcacccgcca ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct
     7321 tgttcttcga acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg
     7381 gcgctatttc gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca
     7441 aattaccggc ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag
     7501 aaacgcaaga cgagaatggc tttaatatct acattctcga cgaaagcaac cgggttgagg
     7561 tgtatcacca ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact
     7621 cgtcatcgca cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt
     7681 tctatcagat tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga
     7741 ttggcaacct gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt
     7801 cgtgatgagc gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact
     7861 gtttcacccg cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg
     7921 cgatcgcaaa tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc
     7981 agccacacct ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag
     8041 gtaatggtca gtacgccgcc gttgatttcg cagtcgatat cgctgtcgcc atccctgtcg
     8101 tccagacgct cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg
     8161 ttcattgttg taccctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct
     8221 tcaaagtttg cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga
     8281 tgtctctgtt ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg
     8341 gggagggcgt aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag
     8401 cgaagcgatc gtttttagtt agcggaccgt taactggaat aatttgcaag ctctttgacc
     8461 gtaattggcg gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca
     8521 agtctggcgt atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg
     8581 ttacatacag ccgatcttta tattgcgcaa tattgccagt aatcagatgg aattgttcag
     8641 gttcaatacc tggtaggtct atccacttgc tccactgacg gtcgggcgtg agctgtttac
     8701 gccagcgaac gcgactttcc cctacttcaa aagagacgtc atcatctacg ggtatcgctt
     8761 tgcctgataa ttgaccaaag ggataacgat gcagaccctt attgtcgcga aaaaccagca
     8821 gtgaatgagg tatccagcga attatctcaa atgagtctgc attcgccgta aggtgaacgc
     8881 catttaaata aatattctgc ccactgcgga taagcagatc attggaatat gaatttctgt
     8941 ttacactcat aagacgtgga ttaatacccc accatctttt ttctaataca gtaacatcac
     9001 tgctgctccc ctgacgtcga ccggacaagt agagattttt actatccacc agcgtggtag
     9061 agttaccttc gatagctttt agtgtcgcca gatcaacacg tgacgcgccg ctattacttt
     9121 cggtgcgctg tccgtcaaaa tagaggctat atttatcgac cgcgaagcgg cccatagcct
     9181 gaaagctggc aatatcgaca gtgggtgttc ccggtggatt gctaacgatt ttgccacgcc
     9241 agagaataac gcggccgtct gtcacccagt ggtattcctc attttctatg cttatttcat
     9301 cggaaggtat ataacccgtt ttcccagaga tatttttcac aaggcgcaaa ccggccctgc
     9361 tcatctccgg aaactgggca atgattttct tatcccccca ataaccttta ccgtaagaca
     9421 taacaccgtg atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg
     9481 tgtgtcccaa caggatgagt gcaactgtga ttatttgacc cttcattttg tcacctcatt
     9541 gcgcggggca ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt
     9601 gtaatttacg ccatagacat atcccgaatg ttgttccttc cagattaagc gaacttgttt
     9661 cggatcagcg gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt
     9721 atcgcgggca taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc
     9781 cggagaacgg ttatccagta agatcagcgg tccgtcagat tcaaacacgt gtaaaccgga
     9841 atggtatggc tacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa
     9901 acgtttgttg ataataagat tgggatcatc aagtttcacg atctccagct tacggtcacc
     9961 agactctgtt tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat
    10021 gggttgaaac tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt
    10081 acgccagaac actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc
    10141 atcccacgcc agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca
    10201 ggtcaagagt gagccaggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag
    10261 ctgttcgccg ttgagcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa
    10321 tggtacggca atgcaggggt taaacgtggt ggaaaacttt cctcgctgat cccaggactt
    10381 ctgcgccagc acacggaaac tctcggggtc agcgaggcga tgcccattga tatacaagaa
    10441 attagcgtca cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac
    10501 ctgatgcagc gttttgatat ccaagctatt tccaccacca ttgtcatcgg tgcgcttgcc
    10561 ttcaaaatag agactgtgtt tatctgcggc aaaatcaccc catgccttga acgaggcgac
    10621 atcgacggga ggtgtgccgg gagggttttg cacaacttca cccgcgtaga ggatattgcg
    10681 accatcgcta tgccatgaat aatgaggata ctaggcctgt gcgccctcat agaggtagtc
    10741 ggttatcaaa tcagtcagag gcggttcttt atcccattcg tctagtgttg tcgggtctat
    10801 taataaattg ggtaaccgac gcaaactctt gtaattcacg tcaggtagag aaagatttgt
    10861 ttctttataa gggcgcaggt acaccacgcc ttttccctgt ttctggtaaa tattatgttc
    10921 aggtgcggaa agccgacatc cccatgccga cgataaaaac aatgaacaag ccaacaaaca
    10981 aaccacattg cgatagtgca taaagccatc ctggcgcgag gtgttgatca cgaaactgcc
    11041 agcagaacat aaatcctcac gagtaagcgt tatactcgca gcatttcctc acttttcaga
    11101 cttcataaag agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag
    11161 aaagcgatga actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat
    11221 tacttactct cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg
    11281 cagataaaaa cgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc
    11341 cggataaaaa cgatcgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag
    11401 tttctgtacc cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca
    11461 acgattttat ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc
    11521 gtcgcctggc ggatcggcac ctgggggtag ggtttgacca gctgctggtg gttgagccgc
    11581 cgtatgatcc tgaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg
    11641 cgcagtgcgg taacggtgcg cgctgctttg cccgttttgt gcgtctgaaa ggactgacca
    11701 ataagcgtga tatccgcgtc agcaccgcca acgggcggat ggttctgacc gtcaccgatg
    11761 atgatctggt ccgcgtaaat atgggcgaac ccaacttcga accttccgcc gtgccgtttc
    11821 gcgctaacaa agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg
    11881 gcgtggtgtc gatgggaaat ccgcattgcg tgattcaggt cgatgatgtc gataccgcgg
    11941 cggtagaaac gcttggtcct gttctggaaa gtcacgagcg ttttccggag cgcgccaata
    12001 tcggttttat gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg
    12061 caggagaaac ccaggcctgc ggcagcggcg cgtgtgcggc ggtggcggta gggattcagc
    12121 aaggtttgct ggacgaagaa gtacgcgtgg aactccccgg cggtcgtctt gatatcgcct
    12181 ggaaaggtcc gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat
    12241 ttattcatct atgaagcaac taggggaaga actgcaggaa acactcacgg agcttgatga
    12301 ccgggcggtt gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc
    12361 agtagaagcg atacgtgtgc cgcatccggt acgcggcacc gtttcgttgg tcgagtggca
    12421 catggcccgc gcacgtaatc acattcatgt tctggaagag aacatggcgt tgttgatgga
    12481 gcaggctatc gccaacgaag ggctgtttta tcgcctactc tacctgcagc gcagtctcac
    12541 cgccgccagc agtctcgacg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg
    12601 cctggctggt gcgagtctgc gcctgtttcc agatcgctgg cgcttaggtg cgccgtcgaa
    12661 ccacactcat ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtttggg
    12721 gcaggaacag cactatcttg ggccgcttaa cggaccggag ctgctggtgg tgctaccgga
    12781 agcgaaagcg gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggtgt
    12841 tgtgctgttt accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact
    12901 tcatgaaatt gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc
    12961 gatttacaca ccgatgtaga acgctaccta cgttatctga gcgtggagcg tcagcttagc
    13021 ccgataaccc tacttaacta ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa
    13081 aacggcctgc aaagctggca acaatgcgat gcagcgatgg tacgcaattt tgctgtacgc
    13141 agtcgccgta aagggctggg agcagcaagt ctggcgttac ggctttctgc gctacgtagc
    13201 ttttttgact ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggagtttcg
    13261 gcaccgaaag cgccgcgtca tctgccgaaa aatatcgacg tcgacgatat gaatcggctg
    13321 ctggatattg atatcaacga tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg
    13381 tacggcgcgg gtctgcgtct ttccgagctg gtggggctgg atatcaaaca cctcgacctg
    13441 gagtccggcg aagtgtgggt gatggggaaa ggcagcaaag agcgccgcct gccgattggt
    13501 cgcaacgctg tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa
    13561 gacgacgcgc tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa
    13621 cgctttgccg aatggggcat aaaacaaggg ctgaataatc acgttcaccc acataaatta
    13681 cgtcactcgt tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag
    13741 ctactgggtc atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac
    13801 cttgcctcgg tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt
    13861 accggccttt ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata
    13921 accgtccggt gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc
    13981 cggcgctgcg cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag
    14041 cggaacccga gatttatcac gacgtgacgc gttggcgttt tcgttcgatt gaacaagcga
    14101 tgctcgacgc cgggctgagt gccgaagaag ccagtgcagg cgcacacgca gcaatgatta
    14161 actttgccaa atggcgcagc cgaatcgacg tcccgcagca aactcacgac accttaaaac
    14221 agctggcgaa gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt
    14281 ttggtttggg ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac
    14341 cgttcagcga tatgtacttt ttggctgcgg aaaaactcaa cgtgccgatc ggcgagatct
    14401 tacatgttgg ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg
    14461 cttgttggat cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc
    14521 cacatctgga aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt
    14581 atatataccc agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc
    14641 ggcggtgcca atggacgttt cttacctgct cgacagcctt aatgacaaac aacgcgaagc
    14701 ggtggccgcg ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg
    14761 cgtactggtg catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat
    14821 tatggctgtg acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcggacaact
    14881 gatgggcacc agccagggcg gcatgtgggt aggcaccttc cacgggctgg cgcaccgcct
    14941 gctgcgtgcg caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga
    15001 agaccagcta cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg
    15061 gccgccgcgc caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca
    15121 tcatattcaa agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta
    15181 tcaggaagcg tgcgatcgcg cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca
    15241 cgaactgtgg cttaacaagc cgcatatcct acaacactac cgcgaacgtt ttaccaatat
    15301 cctggtggac gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc
    15361 gggcgacacc ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg
    15421 cggggcgcag gtggagaata ttcagcgttt ccttaatgat ttccccggtg ccgaaattat
    15481 tcgtctggag caaaactacc gctctaccag caatattctg agcgccgcta acgccctgat
    15541 tgaaaacaat aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc
    15601 tatttccctc tattgcgctt ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat
    15661 caaaacctgg caggacaacg gcggggcgct tgccgagtgc gccattctct accgcagtaa
    15721 cgcccagtcg cgtgtgctgg aagaagcact gttgcaggcg agtatgccgt accgtattta
    15781 cggcgggatg cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct
    15841 gattgccaac cgtaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg
    15901 tattggtgac cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact
    15961 ctggcaggca tgtagtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc
    16021 cttacagcgg tttatggaac tgatcgacgc cttagcgcag gaaactgccg atatgccgct
    16081 gcatgtacag actgaccggg taattaaaga ctccggcctg cgtaccatgt acgagcagga
    16141 gaagggcgaa aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg
    16201 ccagttcagc tacaacgaag aagacgaaga tttaatgccg ctgcaggcgt tcctatccca
    16261 tgcggcgctg gaagcaggcg aagggcaggc ggatacctgg caggatgcgg tacaactgat
    16321 gaccatgcac tcggcgaaag gtctggagtt cccgcaggtg tttatcgtcg gtatggaaga
    16381 gggcatgttc ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg
    16441 tctggcctac gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac
    16501 tcgccgtctg tatggcaaag aggtttacca tcgcccgtcg cgctttattg gcgagttgcc
    16561 ggaagagtgt gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca
    16621 gcggatgggt acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgtgtacg
    16681 ccacgctaag tttggtgaag gcaccatcgt caatatggaa ggcagcggtg aacatagccg
    16741 tttgcaggtg gcattccagg gccagggaat caaatggctg gtggcagctt acgcccggct
    16801 ggagtcggtg taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat
    16861 tcagattcaa taaattgcaa cgtcatgtag gcaggataag gcgtttacgc cgcatccggc
    16921 atcagcagca tatttagtat ctgacacaaa gctatcattc taacctttcc gctcgacgga
    16981 aatcatggtg acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg
    17041 tgaaatcgtg ccaccaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca
    17101 ggcaaaatca taaaaatttg tcgcctgctg gctcaacaaa aacacggcat tcccatgttt
    17161 attaatccca acaccgttac gaattttacg tgaggcgacg ttgggatgaa tacgcggatt
    17221 aattac
//
LOCUS       DAJTNM010000079        17103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTNM010000079 DAJTNM010000000
VERSION     DAJTNM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.079"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..170
                     /locus_tag="OO172_002714"
     CDS             <1..170
                     /locus_tag="OO172_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5512843.1"
                     /translation="SRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAV
                     FGGELTLVQLQ"
     gene            complement(171..362)
                     /locus_tag="OO172_002715"
     CDS             complement(171..362)
                     /locus_tag="OO172_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5512844.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(467..703)
                     /locus_tag="OO172_002716"
     CDS             complement(467..703)
                     /locus_tag="OO172_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5512845.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDARIMRLA"
     gene            complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="OO172_002717"
     CDS             complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="OO172_002717"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR5512846.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2340..4838)
                     /gene="yebT"
                     /locus_tag="OO172_002718"
     CDS             complement(2340..4838)
                     /gene="yebT"
                     /locus_tag="OO172_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR5512847.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTITIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLMTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKV
                     TGEMTVDPSVVTLLRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVV
                     VPGEKALLQEPDVLTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAI
                     EPQHRELVKGDSKFVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASY
                     PLYANLEKALENSLSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAF
                     DIDLHIKPEYRNLLTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGA
                     SASQRKGDKRILYASETAARAVGRQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLI
                     TARNEVQAKAVLYPEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGN
                     PRRDFELEEATITDSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLS
                     DRVMIAMRISKRYQHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATP
                     PGTPLAPKAQEGKHFLLQESEPKEWREWGTALPK"
     gene            complement(4807..6091)
                     /gene="yebS"
                     /locus_tag="OO172_002719"
                     /pseudo
     CDS             complement(4807..6091)
                     /gene="yebS"
                     /locus_tag="OO172_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6221..6718
                     /gene="msrC"
                     /locus_tag="OO172_002720"
     CDS             6221..6718
                     /gene="msrC"
                     /locus_tag="OO172_002720"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5512848.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6815..7513
                     /gene="proQ"
                     /locus_tag="OO172_002721"
     CDS             6815..7513
                     /gene="proQ"
                     /locus_tag="OO172_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5512849.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7533..9581
                     /gene="prc"
                     /locus_tag="OO172_002722"
     CDS             7533..9581
                     /gene="prc"
                     /locus_tag="OO172_002722"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5512850.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9773..10654
                     /gene="htpX"
                     /locus_tag="OO172_002723"
     CDS             9773..10654
                     /gene="htpX"
                     /locus_tag="OO172_002723"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5512851.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10700..12073)
                     /locus_tag="OO172_002724"
     CDS             complement(10700..12073)
                     /locus_tag="OO172_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5512852.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12250..13041
                     /gene="kdgR"
                     /locus_tag="OO172_002725"
     CDS             12250..13041
                     /gene="kdgR"
                     /locus_tag="OO172_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5512853.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13184..13423)
                     /locus_tag="OO172_002726"
     CDS             complement(13184..13423)
                     /locus_tag="OO172_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512854.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13582..13725
                     /gene="mgrB"
                     /locus_tag="OO172_002727"
     CDS             13582..13725
                     /gene="mgrB"
                     /locus_tag="OO172_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5512855.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13800..14087
                     /locus_tag="OO172_002728"
     CDS             13800..14087
                     /locus_tag="OO172_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5512856.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14757..14900
                     /locus_tag="OO172_002729"
     CDS             14757..14900
                     /locus_tag="OO172_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5512857.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14913..15122
                     /gene="cspE"
                     /locus_tag="OO172_002730"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14913..15122
                     /gene="cspE"
                     /locus_tag="OO172_002730"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5512858.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15288..16097
                     /gene="rlmA"
                     /locus_tag="OO172_002731"
     CDS             15288..16097
                     /gene="rlmA"
                     /locus_tag="OO172_002731"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5512859.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16094..16660)
                     /gene="mntP"
                     /locus_tag="OO172_002732"
     CDS             complement(16094..16660)
                     /gene="mntP"
                     /locus_tag="OO172_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5512860.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4521 a         4135 c         4314 g         4133 t
ORIGIN      
        1 gtagccgctc gcgattaggt gaacgcccaa aagggcaggg aattacaggt gctgatcatt
       61 tctggtttgg tcatacaccg ttgcgacatc gcgtggatat tggcaacctg cattatattg
      121 ataccggtgc tgtctttggg ggcgaactga ctcttgtgca actgcaataa ttaaaaatca
      181 ccatactcct gtgctggtcg ccagaaacca tctataaaat cctcaatcgg aaaacaaccg
      241 ccatggcgga tccgttgatc gctcatagaa taaagacact gctgttccgt gttgtagaca
      301 tccacaacaa tatcttcaca accgccatcc aggtagcaaa caaaaagtac cagcgcgaac
      361 atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag gagagatttt acaacggggg
      421 aataaccagg acaaataacc cgccatgccg acgggttctt tttggatcag gcaagacgca
      481 taatcctggc atcagattgt tgatcgtaac gggtacggta aaggacagag tgttcgccat
      541 ttagcagggc agggatactg gtttcagcat cccaggcatc cagttgcata cataaatcgg
      601 ggtcagattt acaaggaatg gttaacgttc gatcacccgg cgattcaccg tgtaattcgc
      661 cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt catcttcgtg ctctccattt
      721 agctgctgat tgtggtacga ccagtccgat cgcacaattt ttcagcctag ttgaggccgc
      781 cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa
      841 gtttcccgtc ccgcaccagt tcacgcgggt agctgttttt cagtcgcgaa ccaatccgtt
      901 tggctaaacc aatcggctga tgctggaaag taaccaacac gtcatccgcc actggcgcgg
      961 cttgcgggta aacatcgcgc ccgcgatacc actcctccgc ttcctgcggt gtcagttcaa
     1021 aagcgtttac attgtcgggg gaggcatggg caataaccgc ttcatgctgc cagcgataac
     1081 ctttgttatg cgtttcggca agtttaatcc ctaagcgaga aaatcggact ttaccgatca
     1141 gggcttcaat gcctaccggg aacaaccaca gttctttgtc gcgttgccag agtcgcaggt
     1201 ttccatccca gtttaagcca acacttgcag ccgcctgacg aatttgtccg gcttcgcgat
     1261 ctttcaccgg gctgaacggg aaattgccca ctttgtattt tggggcgggt aaggcgggga
     1321 tcgcctgagt tttacgcaga cgagcaacga agaagccttc gcagtcgtaa atttgtggga
     1381 aaacatgcaa aaaaccttct tcggtcagcg ccttgtttgc accagggaag agatcgccaa
     1441 gtggtaaaaa ctctacttcg tcggggtaag tctctttcag ccaccggcaa acggcttcgt
     1501 tttcttcccg gtttaaggta caggtcgagt aaaccagcgt accgccagga cgtaatgcat
     1561 gaaaggcgct gtcaatcagt tcccgttggg tagctgcgat ttcctgattg ctttcgggtg
     1621 accagttttt tagcgcatcg ggatctttac gcaccacgcc ttcgccggag cagggagcgt
     1681 ccagcaaaat ggcatcaaac atttctggca ctgacgcacc aaacacgcgg ccaccaaaat
     1741 gtgtgagcgc aacattactg atgccacagc ggctgatatt ggcatgtaac acttttaccc
     1801 gactggcgga aaactcattg gcaaggattg ccccttcgtt attcatccgc gcggcaattt
     1861 gcgtcgtttt tgagcctggc gcagcagcga catccatcac ccgctgtggt gcattaccgt
     1921 cagcaaacaa ggcggcgacg ggcaacattg aactggcttc ctgaatataa aacaggccac
     1981 ttaaatgctc ggtggtacta cccaatggca atgcatcttc attgtcgcgt tcaatccaga
     2041 aaccttcttc acaccacgga attggcgtaa gcgtccagcc ataaggagcg gttaattgca
     2101 ggaaatcagc aacggagatt ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct
     2161 gacaggcggc aagaaaatca tcaaatgaga gcgtcgaagg catagcttcg cgcatttgtg
     2221 tcagaaacgc gtccgggaaa taaacggcgt gttgggccac gagcatgtac cactggcaaa
     2281 aaaacaggcg cgcagtttag cataaacgct ccggcgcagg cacgccggag cagtgggcat
     2341 tatttgggaa gcgcagttcc ccattcacgc cactctttcg gttcactttc ctgcaacagg
     2401 aagtgtttgc cttcctgggc tttcggtgca agtggcgtac ccggaggcgt ggcgaaggcg
     2461 atgccgccac ggataaattg gttaaaggtg ccggttttca ctacgccgcc cgtcagacca
     2521 aagtccagac tgtaacccga tgccaaccag aagacggaat tgttacgcac caggtgttga
     2581 tagcgtttac tgatgcgcat cgcaatcatc acgcgatctg acaatgtccc cagcgtcatt
     2641 ccggtaaccg tacctacttc cagaccacgg aacagcacag gcgtaccgat gcctaacgaa
     2701 ccggcttccg gcgcttcaac aataatgctt aaaccatcta ggtaacgcga atcagtaatg
     2761 gtggcctctt ctaattcaaa gtcgcgacga ggattgccac ggcctggttc gacgttgata
     2821 tacggctgga ggatagtatc tagatgctca acgccagctg ccgagatttg cggtgtgacc
     2881 actgagaagc gcgtaccacc gcgagcaaag gtctggacat attccggata gagcaccgcc
     2941 tttgcctgta cttcattgcg tgcggtaatc agatccagcg tctggatttg cccgatatca
     3001 ataccgagat agcgaattgg catcccgacc gccagttttc cggcatcgaa agcgtgaagc
     3061 gtaatctgcc tgccaaccgc acgggccgct gtttcggaag catacagaat tcgcttgtcg
     3121 cctttacgct gactggcgct ggcaccactg aggttatcga agctaatggc cccctttaat
     3181 gctctggaga gcggggatgc ctgtacggtc aggccactac cattcagctg aactttcgcc
     3241 ccgccttctg cccagaacac gctgttgctg gtcaaaaggt tgcgatattc cggtttaatg
     3301 tgcagatcga tatcgaacgc gttagctcgc ggacgcacgg taataacttc accaacttca
     3361 aatttacggt acagcactac cgatcctgcc tgcacatccg gcagcgtctc tgcactcaaa
     3421 ctcacggttg tggtgggtaa atcgctaagg ctgttctcca gcgctttttc cagattggca
     3481 tacagtggat agctggcttt catctcgcct ttatcgcccg gcagaatacg tatcccgccg
     3541 ttaatccatt ctgaggcgct ggcaccgaga aactcaacgc catccagccc caccttcacg
     3601 tcgacacggc tgttgacgac aaatttgcta tcgcctttta ccagttctcg atgctgaggc
     3661 tcgatggcga cggtaaaggt gacgcctttg ctggtgagtt tacgatcgat aacctggcct
     3721 acctgcacgc cgtgaagaat gagcggctga cccgcatcaa taccgtaact ttccggtgct
     3781 gtcagggtca gtgtcagaac gtccggctct tgcagcaggg ctttttcgcc cggcacaaca
     3841 acgaactctt tgcgtggctc gccatcgccg ggcaccaact caaaggtttt tccggtcagc
     3901 agggcgctga gattagcatc gctaagggat aatttcgggt tgcgtaattc tatgcgggta
     3961 ttttcacgaa gcagggtaac gacgctggga tcaacggtca tttccccagt gactttacca
     4021 ccaggattta aatccagttt agtcagctgt ccgacttcca gcccctgata cattaacggc
     4081 gtcgagtcgg cggttaatcc tgctccactc ggcagttcca gtttcacctt cgcgccactg
     4141 atactgacgt tggcatcaac gccggaaacg ttccagaaac ggctaccttt tttcaccaga
     4201 tcggtaaaac gccgctcgat caggacatca atcaccacgc cttgcttgtt gggattgatg
     4261 gcatagtcgt agacttttcc caccgggatc ttgcggaaat agaccaatga accgctgttc
     4321 agcgaaccga gatcgggggc ttgcaggtgg atcatcagat cgccattgtc cagccgatat
     4381 ttcggttggg tatcgagcgc gacaaagtga tcctgctctt tacctttacc cggcatcatg
     4441 ccgatatagt tcccaccgac gagggcgtcc agcccggaga cacctgccaa cgatgctttt
     4501 ggcgtcatca gccagaactg agtctcttcg cgcagcgcat ctttcatatc ggacttgatg
     4561 ctgaccttga cttcaatctt acgaagatcg tcgctgaggc tgatatcctg cactgttccg
     4621 acttcaacgc cctgataacg aacaggcgta cggcccggaa caataccatc cgccgacata
     4681 aagtcgatgg tgatggtatt accccggtcc tgataactgt cccaaatcag ccaaccggca
     4741 atcattagcg cgatgaaagg cagcagccag aaaggtgaga tacggcgttt atttttaatc
     4801 tgcgcttcag tcgtcgaagc gggcgtttcc tgactcatgt gcatcccaaa gtaagcggct
     4861 gtccagccat tccacagcaa gaatagtcaa aattacggca gcgccaaaat aaaacgcagc
     4921 cggtcccata gtaaaagcga ggatctgatc gcgattaatc agcgacatgg ttaaagatat
     4981 gacaaacagg tcgagcatcg accagcgtcc aatccaggtc accatccgca gtaacagaat
     5041 gcgtgtgcgt aaaccttgct ggcatttaaa atgaatgctc aacagtaaag tgaacatgac
     5101 gatcacttta gtaaacggta ccagaatact ggcgataaac acgattcccg caaccgcaat
     5161 gttgctactt gccagcgaca taattccgga aagaatcgta tcttcctgcc ggccaccatt
     5221 cagataaatg atagaaatag gcaataggtt ggctggtaac aacaaaacga ttgacgctaa
     5281 cagcgccgcc cagcattttt gcaaactatg acgacgacgc aggcgtagcg ggatatggca
     5341 acgcgggcag agaccacgct gatcaggata gccggtaaaa tggcacccaa gacagacacg
     5401 aagtttctcg tccctacgcg tagcggggcg ctgcggataa tatcgctccc acagctcctc
     5461 aacattaaga tgtgacaacg tcaccgtcgt taaaatcacc aacgccacaa aagagaacaa
     5521 gccgacaccc gcctggatat gggcataatc ctgtacctta tatagaagca acgccaatgc
     5581 cgaccaggta gatatccagc attacccact ctttaagtcg ctcaagcatc agcagtaccg
     5641 gacgtaaatt cattcccagt cggttaccaa accataaata tgctatggag gacaccagaa
     5701 tgaggggggc accaataacg cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt
     5761 tggtcatttg ccagatgcct tgcatgacgt tggcgtcaat acgaatgcct aacagccaga
     5821 tatgcaacag cggttcgccc caggcaaacg gcataagcaa cagcatggtg aaggccattg
     5881 ccgccaggcg cgttagcgac cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac
     5941 aataggcact ttgatgagaa tttatctcgg gcaggctaaa cagcatgtca cattgcgggc
     6001 aacgttggta atcaccacgc ggcagttcct cgccgattgc cctcactgtt atcttttttg
     6061 tcggcgtaat ttgtggtgtg ttaagagcca tgacgtgcca aatgtaataa gaattaacat
     6121 taatcttaac ccatgatggg aatgatcttg agcattaacg catttaatgc ttattttaat
     6181 aggctggcag ttccaggtaa gacaaccaag tgattaaata atgaacaaaa cagaatttta
     6241 cgcggattta aatcgcgact ttaacgcgct gatggcggga gaaaccagtt ttctggcaac
     6301 gcttgcgaac accagtgcgt tgttatacga gcgtctcact gacgttaact gggcaggttt
     6361 ttatttgctt gaggacgata ccctggtact cggaccattt cagggcaaaa ttgcctgtgt
     6421 ccggataccc gtcgggcgcg gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg
     6481 tatcgaggat gttcacgcgt ttgacgggca tattgcctgt gatgcggcga gtaattctga
     6541 aattgttctg ccgctggtgg tgaaaaatca gattattggt gttctcgaca tcgatagtac
     6601 cgtcttcgct cgctttacag acgaggacga gcaaggctta cgtcagcttg tggcacagct
     6661 tgaaaaagtg cttgcaacga cggattacaa aaaattcttt gcgagcgtcg caggataatc
     6721 aacggataac gtagcattta ctggtagcgt cattataatg acgcctgttc atgcctgcgc
     6781 ttgttggcta cgtccgttgt aatcaggaaa tttcatggaa aatcaaccta agttgaatag
     6841 cagtaaagaa gtaatcgcgt ttctcgccga acgttttccc cactgtttca gtgcggaagg
     6901 tgaagcgcgt ccgctgaaaa ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga
     6961 aatgaacctg agcaaaacgc aattacgatc cgctttacgt ctctacactt cgagctggcg
     7021 ttatctttac ggtgttaaac ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg
     7081 tgagctggac gagcaacatg tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg
     7141 tgttcaggca cagcgtgctg aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg
     7201 tgagaaagaa gacgcaccgc gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa
     7261 agaaggcgct gaacgtaaac ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt
     7321 aaaagcacct cgcgaagaac agcacacccc ggtttctgac atttcagctc tgactgtcgg
     7381 acaagccctg aaggtgaaag cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac
     7441 caaagacggc gtccgcgtcc agctgaattc gggtatgtct ttgattgtgc gcgcagaaca
     7501 cctggtgttc tgaaacggag gccgggccag gcatgaacat gttttttagg cttaccgcgt
     7561 tagctggcct gcttgcaata gcaggccaga ccttcgctgt agaagatatc acgcgtgctg
     7621 atcaaattcc ggtattaaag gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt
     7681 cgcgcttcac ccgttctcat tatcgccagt tcgacctcga tcaggcattt tcggccaaaa
     7741 tctttgaccg ctacctgaat ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg
     7801 ttgaacagtt cgcgaaaaag aaaaccgagt taggcgatga actgcgttca ggcaaactcg
     7861 acgttttcta cgatctctac aatctggcgc aaaagcgccg ttttgaacgt taccagtacg
     7921 ctttgtcggt actggaaaag ccgatggatt tcaccggcaa cgacacttat aaccttgacc
     7981 gcagcaaagc gccctggccg aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag
     8041 tcaaattcga cgagttaagc ctgaagctga caggaaaaac ggataaagaa attcgtgaaa
     8101 ccctgacacg ccgctacaaa tttgccattc gtcgtctggc gcaaaccaac agcgaagatg
     8161 ttttctcgct ggcaatgatg gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt
     8221 ccccgcgtaa taccgaacag ttcaacactg aaatgagttt gtcactggaa ggtattggcg
     8281 cagtgctgca aatggatgat gactacaccg ttatcaattc gatggtggca ggtggtccgg
     8341 cagcgaagag taaagctatc agcgttggtg acaaaattgt cggtgttggt caaacaggca
     8401 agccgatggt tgacgtgatt ggctggcgtc ttgatgatgt ggttgcctta attaaagggc
     8461 cgaagggcag taaagttcgt ctggaaattt tacctgctgg taaagggacc aagacccgta
     8521 ctgtaacgtt gacccgtgaa cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga
     8581 agaccgtcgg taaagagaaa gtcggcgtgc tggatattcc gggcttctat gtgggtttga
     8641 cagacgatgt caaagttcaa ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca
     8701 tcgacctgcg tagcaatggc ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt
     8761 ttattcctgc gggtcccatt gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca
     8821 gcgataccga cgggcaggtg ttctataaag gcccactggt ggtactggtt gaccgtttca
     8881 gtgcttcggc ttcagaaatc tttgccgcgg caatgcagga ttacggtcgt gcgctggttg
     8941 tgggtgaacc gacgtttggt aaaggcaccg ttcagcaata ccgttcattg aaccgtattt
     9001 acgatcagat gttacgtcct gaatggccag cgctgggttc tgtgcagtac acgatccaga
     9061 aattctatcg cgttaacggc ggcagtacgc aacgtaaagg cgtaacacca gacatcatca
     9121 tgccgacggg taatgaagaa acggaaacgg gtgagaaatt cgaagataac gcgctgccgt
     9181 gggatagcat cgatgccgcg acttatgtga aatcaggaga tttaactgcc tttgaaccgg
     9241 agctgctgaa ggaacataat gcgcgtatcg cgaaagatcc tgagttccag aacatcatga
     9301 aggatatcgc gcgcttcaac gctatgaagg acaagcgcaa tatcgtttct ctgaactacg
     9361 ctgtgcgtga gaaagagaat aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac
     9421 gctttaaacg cgaaggtaaa ccggagttga agaagctgga tgatctaccg aaagattacc
     9481 aggagccgga tccttatctg gatgagacgg tgaatatcgc actcgatctg gcgaagcttg
     9541 aaaaagccag acccgcggaa caacccgctc ccgtcaagta atatcaatca ggcacaagaa
     9601 attgtgcctg attttttaac agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa
     9661 gttgtgtctt tctggtgact tacgcactat ccagacttga aaatagtcgc gtaacccata
     9721 cgatgtgggt atcgcatatt gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg
     9781 aatcgcgctc ttcctgctaa cgaacctggc cgtaatggtc gttttcgggc tggtactgag
     9841 cctgacaggg atacagtcga gcagcgttca ggggctgatg atcatggcct tgctgttcgg
     9901 ttttggtggt tccttcgttt cgcttctgat gtccaaatgg atggcattac gatctgttgg
     9961 cggggaagtg atcgagcaac cgcgtaacga aagggaacgt tggctggtca atactgtagc
    10021 aacccaggct cgtcaggcgg ggatcgctat gccgcaagtg gctatctacc atgcgccgga
    10081 catcaacgct tttgcaaccg gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg
    10141 tttgctgcag aacatgagcc cggatgaagc cgaggcggta attgctcacg aaatcagcca
    10201 catcgccaat ggtgatatgg tcaccatgac gctgattcag ggcgtggtga acaccttcgt
    10261 tatctttatt tcccgtattc tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga
    10321 tgaaggtgaa gagagcaacg gcaacccgct gatctacttt gcggttgcaa cggttctgga
    10381 actggtgttt ggtattctgg cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt
    10441 ccatgctgat gccggttcgg caaaactggt tggtcgcgag aaaatgattg ccgcgctgca
    10501 gcgcctgaaa accagctatg aaccgcaaga agcaaccagc atgatggctt tctgcattaa
    10561 cggtaagtcg aaatcgctca gtgagttgtt catgactcac ccgccgctgg ataaacgtat
    10621 tgaagctctg cgtacgggtg aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt
    10681 cctgatcgac gcgctttttt tatgccctgg ctcgtggctg agtgatacgt aaaccactga
    10741 cacaggcagc aatcactgcc agaatagccg cagccatcag cgagacgtgc gtaccattat
    10801 caccaaactg atttagcatc agcgccacca gcgccgcgcc actactctga cccagtagac
    10861 gagccgttcc taacatgcca ctggccccac cgctacgttc gcgcggagcg gaggtaataa
    10921 tggtgtggtt attgggtgac tggaataagc caaatccagc gccacataag atcatcggcc
    10981 agataatatt gatatccgca ggtgacgcgg gcagcagaac cagggaaaaa agccccgcag
    11041 ccatgatgaa caaccctaaa gcacccagca atcctgcatg tacgcgttca atcaaatagc
    11101 ctgccagcgg agccatcacc atcgttgcta acggccacgg tgtcagaagt aaacctgttt
    11161 cgacttcact acgcccgagc acggtttgca ggtaaaaggg cagggaaacc attgccagca
    11221 tttgtgcgca gaaagagcaa acagatgtgc aaatagaaag tgaaaacagc gggatacgca
    11281 gtaaatccac cggtagcagc ggtacgggaa gagaaagctg gcggcgaata aagaaaatac
    11341 caacaacaac cattaccacc agttccgcag caattaacgt cagcgattgc ccctgagcga
    11401 aaccactcaa cgcagtgata agcaggccga aggttaacgc gttcatcacg gcgctgggca
    11461 ggtcgaaacg gggtttactg gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa
    11521 gcagggcgat aatacctaac ggtacgttga ttaaaaataa ccatttccag gatgcgatgg
    11581 agaggattgc tgcagcaatt gtcggcccgg cagcagaaga gacggcaaca ataaacgagt
    11641 ttatgcccat ccctctcccc agaaaacgtt gtggatagat caggcggata agtgcggtat
    11701 taacgctcat caacgctgca ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt
    11761 gcagcgaatc agaaagggcg cagaacagtg aagacaacag aaaaacgacc agaccgcatt
    11821 tataaatacg tcgatagcca aacatatcgc ccagaaacga aaacgagagc agggagatga
    11881 caatggcgat ttgataggcg ttcactaccc agatggaact ggctggtgtg gcatgaaggt
    11941 ccgtggcgat tgttggcagg gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa
    12001 taccaatcac aatggttaat atcgcaccgt atcgctgggg caatggcagg ccgtcggcct
    12061 gaacttttgg cataggaatt ttatatcttt ggtgaataat caacaggggt atgattctag
    12121 cactgatatt cacggtggat gtcgcagaat tgtaactaac cggcaaaaga tggattgcga
    12181 ccaccctggt gcgaaattat aataaaaaac agttctgatt tttataaaac actcgcaatg
    12241 aggtgataaa tggctaacgc agatctggat aaacagcctg attctgtatc ttccgtgcta
    12301 aaagtttttg gcattttgca ggcgctgggt gaagagcgcg aaatagggat aaccgagctg
    12361 tcgcagcgcg tcatgatgtc aaaaagcacc gtttatcgct ttttacagac catgaaaacc
    12421 ttaggttatg tggcgcagga aggggagtcg gagaaatatt ccctgaccct gaaattgttt
    12481 gaactgggcg ctcgcgcgtt acaaaacgtc gatttaattc gtagcgcaga tatccagatg
    12541 cgtgagatct cccgcctgac caaagaaact atccacctcg gcgcactgga cgaagacagt
    12601 attgtttaca ttcacaaaat tgactctatg tacaatttgc gcatgtattc acggattggg
    12661 cgtcgtaatc cgctgtacag caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc
    12721 gatgaagtga agcaaattct tgagggcgtg gagtataaac gcagtaccga gcggaccatc
    12781 accagtacag aagcgttatt acccgttctg gaccaggtgc gcgagcaggg gtatggcgaa
    12841 gataacgaag agcaggaaga agggctgcga tgcattgcgg taccggtatt tgatcgcttt
    12901 ggcgtggtca ttgccggttt gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt
    12961 ttacaggaat atgtcgcaat gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt
    13021 tatcacgact atccgttctg atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt
    13081 ctttatttag catgagaaaa ataaagttga aggtgcgtta tattaaacgc gcttgctata
    13141 agaatatttt actcatgagt gagaatctgg ttatttatta cccttaacca ttatcgacca
    13201 cgatattgct tttacgtaac agtgggcaat ctgttatccc caagaaacca cttttagtgt
    13261 gcaagtattg taccgtgctg gtgcctctgg ccgtcagata ggtacattgc aaacctaatc
    13321 ctgcggcatt ctctttgctt ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc
    13381 ataccagtgc tatcagcata actgtgcgaa tgatgaatcg cattacaatc tcttctcttt
    13441 ttatgttcgc ttaatcgtag cggcaatatg cgctgaagca agctactcat tccgaaaaag
    13501 cacgaatatc gacatagtta ggcgctgttt aactaacgca tgctagttta atgacataag
    13561 gtaggtgaag cggagattgg agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg
    13621 ttggcttgct tgctgctttg ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa
    13681 tttttcagcg gaatttgtgc cattaaccag tttatcccgt ggtgatatca ttttttcagc
    13741 actagatttt cttcctctcc agcagtggta gaatagcgcc tctactcaga ggtggtgaaa
    13801 tgaacgaagt tgtaaattca ggcgtgatga acattgcgtc tttggttgta tcggtggtgg
    13861 ttcttcttat cgggctcatc ttgtggtttt ttatcaatcg tgccagttca cggactaacg
    13921 aacagattga actgcttgag gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac
    13981 gtcgtttgtg cgaagcaaac gaaccagaga aagcagataa aaagaccatt gagagtcaaa
    14041 aatcggttga tgacgaagat attattcgcc tggtcgccga acgataatcc ttcctcaatc
    14101 ttgagtaacc ccgtcgttga agggttactc aattcatcct gattatacgc actgtcatgg
    14161 tgcaactgct tcaaattaaa actctggtct gacttcaaat aattatcata ttatttattt
    14221 gcgtaacggg atatcagaaa aagatatttt cctcattaat ttattaggat gtttacatca
    14281 gatttgtgat taagcgtggt attatttatt agcgaaacgt ttctctcttg atttttttgc
    14341 tcactcatca atttttctta ttttaaattt acaatccctt tggggattga cttctcttta
    14401 gggtaattga tagccgttaa ctgactgttt tatgagaaaa agtgatataa ctttttattc
    14461 attgcatagc agaaaatgtg atattgcacg cactatgtaa taacttctcc ctctggcctg
    14521 gaacaactga acttattgaa ctatgttaga aactacgcca gtttaagtat ctgcctgaac
    14581 tggcaaggtt aagcacaatg atatatcggc gcgtattccg ttgcataagt gtgcaaaaaa
    14641 agtggaagac gtatcgagat ttgtgcgtct gatcgagaca tgtttaaaaa tggcttgcca
    14701 taattaacgt tgtatgtgat aaaagatttc gggttaaacg aggtacagtt ctgtttatgt
    14761 gtggcatttt cagtaaagaa gtcctaggta aacacgttga cgttgaatac cgcttctctg
    14821 ccgagcctta tattggtgcc tcatgcagta atgtgtcagt tttatctatg ttatgcctgc
    14881 gggcgaagaa aacaatctaa ggaatttttc aaatggcaaa gattaaaggt caggttaagt
    14941 ggttcaacga gtctaaaggt tttggcttca ttactccggc tgatggcagc aaagatgtgt
    15001 tcgtacactt ctccgctatc cagggtaatg gcttcaaaac tctggctgaa ggtcagaacg
    15061 ttgagttcga aattcaggac ggccagaaag gtccggcagc tgttaacgta acagctatct
    15121 gatcgaatcc actgatctga agtgtgaata cgcttcaatc tcgctataaa gcctcgtcga
    15181 atgcgaggct ttttactatg ctttatcttc gctcctggcg ttcggatatt tgccagccac
    15241 gtgattcgcg ttacacttgc ggcctttagt atcctgccgg agttgtcatg tcgttttcct
    15301 gtccactttg ccatcagcct ctttcgcgtg aaaaaaacag ctatatctgt ccccaacgac
    15361 atcagtttga tatggcgaaa gaagggtatg tcaatctgct gcccgttcag cataaacggt
    15421 ctcgtgatcc gggcgacagc gcggaaatga tgcaagcacg ccgcgcattc ttagatgccg
    15481 gacattatca gccgctgcgt gatgcaattg tcgcccaact gcgggaacgg cttgatgaaa
    15541 aggccacggc ggtgctggat attggctgtg gtgaagggta ttacacacac gcatttgccg
    15601 atgcgttgcc cgaaatcacc acgtttggcc tggatgtctc gaaggtagcg ataaaagcgg
    15661 cggcgaaacg ctatccgcag gtcactcttt gtgtcgcttc cagccaccgt ttgccgtttt
    15721 ccgataccag tatggacgcc ataatacgta tttacgcgcc gtgtaaagca gaagaattag
    15781 cacgagtagt gaagcccggc ggctgggtca ttactgccac gccgggaccg cgacatttga
    15841 tggagctgaa ggggctgatt tacaatgaag tacatcttca tgcacctcat gcagaacaac
    15901 tggaaggttt tacattacag cagagtgcgg agttgtgtta tccgatgcgt cttcgcggtg
    15961 atgaagccgt cgcattattg cagatgacgc cgtttgcctg gcgtgcgaag ccagaagtct
    16021 ggcaaacact ggcagcaaaa gaagtgttcg actgccagac ggactttaat attcacctct
    16081 ggcagcgttc ttattaaccg tggaagtgcg tccagaggat ctggacgccg atgccgatca
    16141 gcaccagccc gccgagaatt tccgcttttt tcccaataat tgagccgata aagcgaccaa
    16201 ccatcatccc taatgtcgac ataatcaagg ttgcacaacc aatggccaat gcggtcgcga
    16261 taatgttgac ctgaaggaaa gcaagaccaa cacccacagc catggcatcc aggctggtgg
    16321 caatcgcggt ggttaccagt agccagaaac cgtgccgacg gcgcggctct tcatcttcat
    16381 catctgagcc acgaaaaccc tcaataatca ttcgcccgcc gaggaatatc agcagcacaa
    16441 acgcaatcca gtgattccat tcaaggacaa accggctggc taacatgccc attccccagc
    16501 cgatcagcgg cgtcagggtt tcgacggcac caaaaataag gccggttcgc aatgcttcag
    16561 aaaatttcgg tttatggagg ggggcacctt taccgattga tgcagcaaat gcatccatcg
    16621 acataccaaa cgcaagaaga acagtagcag tgatattcat gacaacgtcc tgaccggggt
    16681 atccataaaa cacatcatcg cccccagcaa acatgcatac atgaacaaca agccgcgatg
    16741 ggttttcgcg ttcatgcagg catagcgatt gatgtgcata tggtctcgcc tgactgaaag
    16801 tcttcagtcc gtacgtgcca cgttttgcaa cgagtatgtt gacacgtaca tttccggaat
    16861 ctggaaatcg gctactcccc aaatgagggc gcaaccttaa catattttga gaatataaaa
    16921 caactgttgg aatatattat ttcattgctt tattgataac gattttcatt tatgtttata
    16981 tatcaatgaa acgttaaaat aaataacaaa taagggaaga gataatatag cctaagctat
    17041 gtctgattat taaaaataca ctaaacatag ctttggctaa attcataagt tattgagttg
    17101 ata
//
LOCUS       DAJTNM010000080        16618 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTNM010000080 DAJTNM010000000
VERSION     DAJTNM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.080"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..942
                     /locus_tag="OO172_002733"
     CDS             <1..942
                     /locus_tag="OO172_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HCR5512861.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
     gene            942..4091
                     /gene="mdtB"
                     /locus_tag="OO172_002734"
     CDS             942..4091
                     /gene="mdtB"
                     /locus_tag="OO172_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR5512862.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVALSTLLLSVLLWVFI
                     PKGFFPVQDNGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTN
                     PSLNSARLQINLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRT
                     QYQFTLQATSLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLG
                     ISMADVDNALYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGV
                     VPLSSIAKIEQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVD
                     ITTQFQGSTLAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGA
                     LLALMIAGSELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLL
                     RFRPILMTTLAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLL
                     FDRLVLWTKSRFARHEEEA"
     gene            4092..7157
                     /gene="mdtC"
                     /locus_tag="OO172_002735"
     CDS             4092..7157
                     /gene="mdtC"
                     /locus_tag="OO172_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5512863.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLRITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            7158..8573
                     /locus_tag="OO172_002736"
     CDS             7158..8573
                     /locus_tag="OO172_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5512864.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            8570..9973
                     /gene="baeS"
                     /locus_tag="OO172_002737"
     CDS             8570..9973
                     /gene="baeS"
                     /locus_tag="OO172_002737"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5512865.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            9970..10692
                     /gene="baeR"
                     /locus_tag="OO172_002738"
     CDS             9970..10692
                     /gene="baeR"
                     /locus_tag="OO172_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5512866.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            10883..11215
                     /locus_tag="OO172_002739"
     CDS             10883..11215
                     /locus_tag="OO172_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5512867.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            11362..12723
                     /gene="yegQ"
                     /locus_tag="OO172_002740"
     CDS             11362..12723
                     /gene="yegQ"
                     /locus_tag="OO172_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5512868.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(13065..13382)
                     /locus_tag="OO172_002741"
     CDS             complement(13065..13382)
                     /locus_tag="OO172_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512869.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            13788..14687
                     /gene="yegS"
                     /locus_tag="OO172_002742"
     CDS             13788..14687
                     /gene="yegS"
                     /locus_tag="OO172_002742"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5512870.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(14769..15542)
                     /locus_tag="OO172_002743"
     CDS             complement(14769..15542)
                     /locus_tag="OO172_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5512871.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(15648..>16618)
                     /gene="gatD"
                     /locus_tag="OO172_002744"
     CDS             complement(15648..>16618)
                     /gene="gatD"
                     /locus_tag="OO172_002744"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5512872.1"
                     /translation="QDEVRVKIASSGLCGSDLPRIFKNGAHYYPITLGHEFSGYIDAV
                     GSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEYIVVKRK
                     NVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCENKNVIIIGAGTIGLLAIQCAVALG
                     AKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVPQT
                     VELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWE
                     TASRLLTERKLSLDPLIAHRGSFESFTQAVRDIARNAMPGKVLLIP"
BASE COUNT         3831 a         4453 c         4372 g         3962 t
ORIGIN      
        1 accgggcttg gcaccattac cgccgctaat actgttacgg tgcgcagccg cgtggacggt
       61 caactgatgg cgttacattt ccaggaaggc cagcaggtca aagcaggcga tttactggca
      121 gaaattgacc ccagccagtt caaagttgca ttagcacaaa cccagggcca actggcaaaa
      181 gataaagcca cgcttgctaa cgcccgccgt gatctggcgc gttatcagca actggcaaaa
      241 accaatctcg tctcccgtca ggagctggat gcccaacagg cgctggtcag tgaaaccgaa
      301 ggcaccatta aggctgatga agcaagcgtc gccagcgcgc agttgcaact cgactggagc
      361 cgcatcaccg caccagtcga tggtcgcgtt ggtctcaagc aagttgatgt tggtaaccaa
      421 atctccagtg gtgataccac cggaattgtg gtgatcaccc agacgcatcc tatcgattta
      481 gtctttaccc tgccggaaag cgatatcgct actgtagtgc aggcgcaaaa agccggaaaa
      541 ccgctgatgg tagaagcctg ggatcgcacc aattcgaaga aattaagtga aggcacgctg
      601 ttaagcctcg ataaccaaat cgatgccaca accggtacga ttaaagtgaa agcacgcttt
      661 aataatcagg atgatgcgct gtttcccaat cagtttgtta acgcgcgcat gttagtcgac
      721 accgaacaaa acgccgtagt gatcccaaca gccgccctgc aaatgggcaa tgaaggccat
      781 tttgtctggg tgctgaatag cgaaaacaag gtcagcaaac atctggtgac gccgggcatt
      841 caggacagtc agaaagtgga agtggtggaa gcccagagcg ccaccactcc ggaagagaaa
      901 gccaccagcc gcgaatacgc gaaaaaagga gcacgctcct gatgcaggtg ttacccccga
      961 gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga
     1021 tggtggcgat cttactcgcc gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc
     1081 cggaagtgga ctatccgacc attcaggtgg tcacgctcta cccaggtgcc agcccggatg
     1141 tcatgacctc tgccgttacc gcgccgctcg aacgccagtt cgggcagatg tctggcctga
     1201 aacagatgtc gtcgcaaagt tccggcggtg cgtcagttat cactttgcag ttccagctaa
     1261 cattaccgct caatgtcgcc gagcaggaag tgcaggccgc gattaacgct gcgaccaact
     1321 tgttgccgag cgatctgcct aacccgccgg tttacagcaa agtgaacccg gcagatccgc
     1381 cgatcatgac gctcgccgtc acctcaaccg ccatgccgat gacgcaagtg gaagatatgg
     1441 tggaaacccg cgtcgcgcag aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt
     1501 ccggcggtca gcgtccggct gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg
     1561 gcctgaccag cgaaaccgtg cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag
     1621 gtagcctcga cggcccttcc cgtgcggtca cgctttccgc gaacgaccag atgcaatccg
     1681 ccgaagagta tcgccagcta atcatcgcct accagaacgg cgcgccaatt cgtctgggcg
     1741 atgtagcaac tgtagagcaa ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag
     1801 aacaggccat tgtgatgaat gttcagcgcc agcccggtgc taacattatc tccaccgccg
     1861 acagcattcg gcagatgctg ccacagctca ctgagagtct gccgaaatcg gtgaaggtga
     1921 cagtgctttc cgatcgcacc accaatatcc gcgcatccgt cgatgatact cagtttgaat
     1981 tgatgatggc tatcgcgctg gtagtcatga ttatctacct gtttttgcgc aatattccgg
     2041 cgaccatcat tcccggtgtt gctgtaccgc tgtcgttaat cggcactttc gcggttatgg
     2101 tgtttctcga tttttcaatc aataacctga cactgatggc gttaactatc gccaccggat
     2161 tcgtggtcga tgacgccatc gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg
     2221 aaaaaccgtt ggcggcggcg ctcaagggcg caggtgaaat cggctttacc attatctcgc
     2281 tgaccttctc actgattgcg gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc
     2341 gactgttccg cgaatttgct attaccctgg cggtagcgat tttgatctca gcggtggtgt
     2401 cgctgaccct gacaccgatg atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac
     2461 agaaccgctt ctcccgtgcc tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc
     2521 gtggactggc gaaagtgctg aatcatccgt ggctgacctt aagcgtggca ctcagcacgc
     2581 tgctgcttag cgtggcactc agcacgctgc tgcttagcgt gctgctgtgg gtgttcattc
     2641 cgaaaggttt cttcccggta caggacaacg gcattattca gggcactttg caggcaccgc
     2701 aatccagctc ctttgccaat atggcccagc gacaacgcca ggtcgcggac gtgattttgc
     2761 aggatccggc agtgcaaagc ctgacctcat ttgttggcgt tgatggcact aacccgtcgc
     2821 tgaatagtgc acgtttacaa atcaacctca aaccgttgga tgaacgtgat gatcgggtgc
     2881 aaaaagtcat cgcccgtctg caaacggcgg tagataaagt gccgggcgtc gatctcttcc
     2941 tgcaaccaac gcaggacctg accatcgata ctcaggtcag ccgcacccag taccagttta
     3001 ccttgcaggc cacgtcactg gatgcgctca gtacctgggt gccacagttg atggaaaaac
     3061 tccagcaact gccacagctt tctgatgtct ccagcgactg gcaggacaaa gggctggtgg
     3121 cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg gatcagcatg gcggacgtcg
     3181 ataacgccct gtacaacgcg tttggtcagc gactgatttc cactatttat actcaggcta
     3241 accagtatcg cgtggtgctg gagcacaata ccgaaaatac cccaggcctc gcggcgctgg
     3301 ataccattcg cctgaccagc agcgacggtg gcgtggtgcc gttaagctca attgccaaaa
     3361 ttgagcagcg ttttgcgccg ctttccatca accatctgga tcagttcccg gtaacaacca
     3421 tctcctttaa cgtgccggat aactattcgc tgggcgatgc ggtgcaggcg attatggaca
     3481 ccgaaaagac gctgaatctg ccggtggata tcaccacgca attccaaggc agcaccctcg
     3541 ccttccagtc ggcgctgggc agcactgtct ggctgattgt cgcggcggtg gtggcgatgt
     3601 atatcgtgct cggcattctg tacgagagct ttattcaccc gatcaccatt ctctcgacgc
     3661 tacccaccgc aggggtcggc gcgctgctgg cattaatgat tgccggtagc gaactggatg
     3721 tgattgcgat tatcggcatt attttgctga tcggtattgt gaagaagaac gccatcatga
     3781 tgatcgactt cgcgctggct gctgagcgcg agcaaggcat gtcgccgcgc gatgcaatct
     3841 accaggcttg tctgttgcgt tttcgtccga tcctgatgac caccctggcg gctctgcttg
     3901 gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga actgcgtcgt ccgttaggta
     3961 tcggcatggt cggcggtctg attgtcagcc aggtgctgac gctgttcacc acgccggtga
     4021 tttatctgct gttcgaccgc ctggtattgt ggaccaaaag tcgctttgct cgccatgaag
     4081 aggaggcgta agtgaagttt tttgccctct tcatttaccg cccggtggcg acgattttac
     4141 tgtcggtcgc cattaccctg tgcggcatac tgggtttccg tatgctgccg gtcgccccgc
     4201 tgccgcaggt cgattttccg gtgattatgg tcagcgcctc gctgcccggt gcgtcgccgg
     4261 agacaatggc gtcttccgtt gccacgccgc tggagcgctc acttgggcgc attgccgggg
     4321 ttagtgaaat gacctccagc agttcgctcg gcagcacgcg cattattttg cagtttgatt
     4381 ttgaccggga tatcaacggc gcagcgcgtg atgtgcaggc ggcgatcaac gctgcacaaa
     4441 gtttgctgcc cagcgggatg cccagccgcc cgacctatcg caaagcgaac ccgtcggatg
     4501 cgccaattat gatcctcacg ctgacatccg atacttattc gcagggtgaa ctgtacgatt
     4561 tcgcctcgac gcagctggct ccgacgattt cgcaaatcga cggtgtcggt gatgtcgatg
     4621 tcggtggcag ctcgctgccc gccgtgcgcg tcgggctgaa cccgcaggcg ctgtttaatc
     4681 agggcgtgtc gctggacgac gtacgcactg ccatcagcaa tgccaacgtg cgtaaaccgc
     4741 agggggcgct ggaagatggc actcaccgct ggcagatcca gaccaacgat gaactgaaaa
     4801 ccgccgctga atatcagccg ctgattattc actacaacaa cggcggcgcg gttcgtctgg
     4861 gcgatgtggc gacggtgact gactcagtgc aggatgtgcg caacgccggg atgaccaacg
     4921 ccaaaccggc tattttactg atgatccgca aactgccgga agccaatatt atccagacgg
     4981 tagacagcat ccgggcaaaa ttaccagagt tgcaggagac cattccggcg gcgattgatc
     5041 tgcaaattgc ccaggatcgc tcccccacca ttcgcgcctc gctggaagaa gtcgagcaaa
     5101 cgctgattat ctcggtggcg ctggtgattc tggtggtctt tttattcctg cgctcgggtc
     5161 gcgccactat tattcccgcc gttgcggtgc cggtttcgct gattggtacg tttgcggcga
     5221 tgtacctgtg cggattcagt ctcaataatc tttcgctgat ggcgctcacc attgctactg
     5281 gtttcgtggt ggatgacgcc attgtggtgc tggaaaacat tgcccgtcat ctggaagcgg
     5341 ggatgaaacc actacaggcc gcgctgcaag gtactcgcga agtcggcttt acggtgctgt
     5401 cgatgagtct gtcactggtg gcggtgttcc tgccgctgct gttgatgggc ggattgccgg
     5461 gccgactgtt acgcgagttt gccgtgacgc tttctgtcgc cattggtatt tcgttgctgg
     5521 tttccctgac gctaacgcca atgatgtgcg gctggatgct aaaagccagc aagccgcgtg
     5581 agcaaaagcg actgcgtggt tttggccgca tgttggtagc cctgcaacaa ggctacggca
     5641 agtcgctgaa atgggtgctc aatcataccc gtctggtggg cgtggtgctg cttggcacca
     5701 ttgcgctgaa tatctcgatc ccgaaaacct tcttcccgga gcaggacacc ggcgtactga
     5761 tgggtgggat acaggcggat cagagtattt cgtttcaggc gatgcgcggc aaattgcagg
     5821 atttcatgaa aattatccgt gacgatccgg cagtggataa tgtcaccggc tttaccggcg
     5881 gttcacgagt taacagtggg atgatgttta tcaccctcaa gccacgcgac gaacgcagcg
     5941 aaacggcgca gcaaattatc gaccgtctgc gcgtgaagct ggcgaaagaa ccgggggcga
     6001 atctgttcct gatggcggta caagatattc gcgttggtgg gcgtcagtcg aacgccagct
     6061 accagtacac gttgttatcc gacgacctgg cggcactgcg cgaatgggag ccgaaaatcc
     6121 gcaaaaaact ggcgacgttg ccggaactgg cggacgtgaa ctccgatcag caggataacg
     6181 gcgcggagat gaatctggtt tacgaccgcg acaccatggc acggctggga atcgacgtac
     6241 aggccgccaa cagtctgtta aataacgcct tcggtcagcg gcaaatctcg accatttacc
     6301 agccgatgaa ccagtataaa gtggtgatgg aagtggatcc gcgctatacc caggacatca
     6361 gtgcgctgga aaaaatgttc gttatcaata acgaaggcaa agcgatcccg ctgtcgtatt
     6421 tcgctaaatg gcaaccggcg aatgccccac tatcggtgaa tcatcaggga ttatcggcgg
     6481 cctcgaccat ctcgttcaac ctgccgaccg gaaaatcgct ctcggacgcc agtgcggcga
     6541 tcgatcgcgc aatgacccag cttggtgtgc cttcgacggt gcgcggcagt tttgccggca
     6601 cggcgcaggt tttccaggag acgatgaact cgcaggtgat cctgattatc gccgccatcg
     6661 ccacggtgta tatcgtgctg ggtatccttt acgagagtta cgtacatccg ctgacgattc
     6721 tctccaccct gccctcggcg ggcgttggag cgctgttggc gctggagctg ttcaatgccc
     6781 cgttcagcct aatcgccctg atagggatca tgctattaat cggcatcgtg aagaaaaacg
     6841 ccattatgat ggtcgatttt gcgcttgaag cccaacggca cggtaacctg acgccgcagg
     6901 aagctatttt ccaggcctgt ctgctgcgtt ttcgcccgat tatgatgacc accctggcgg
     6961 cgctgtttgg cgcgctgccg ctggtattgt cgggcggtga cggctcggag ctgcggcaac
     7021 ccctgaggat caccattgtc ggcggtctgg tgatgagcca gctcctgacg ctgtatacca
     7081 cgccggtggt gtatctcttt ttcgaccgtc tgcggctgcg tttttcgcgt aaacctaaac
     7141 aaacggtaac cgagtaaatg acagatcttc ccgacagcac ccgctggcaa ttatggattg
     7201 tggctttcgg cttctttatg cagtcgctgg acaccaccat cgtaaacacc gcccttccct
     7261 caatggcgca aagcctcggg gaaagtccgt tgcatatgca catggtcatt gtctcttatg
     7321 tgctgaccgt ggcggtgatg ctgcccgcca gcggctggct ggcggacaaa gtcggcgtgc
     7381 gcaatatttt ctttaccgcc atcgtgctgt ttactctcgg ttcactgttt tgcgcgcttt
     7441 ccggcacgct gaacgaactg ttgctggcac gcgcgttaca gggcgttggc ggtgcgatga
     7501 tggtgccggt cggcaggttg acggtgatga aaatcgtacc gcgcgagcaa tatatggcgg
     7561 cgatgacctt tgtcacgtta cccggtcagg tcggtccgct gctcggtccg gcgctcggcg
     7621 gtctgctggt agaatacgcg tcgtggcact ggatcttttt gatcaacatt ccggtgggga
     7681 ttatcggtgc gatcgccaca ttgatgttaa tgccgaacta caccatgcag acgcggcgct
     7741 ttgatctttc cggcttttta ttgctggcgg ttggcatggc ggtattaacc ctggcgctgg
     7801 acggcagtaa aggtacaggt ttatcgccgc tggcgattgc tggcctggtc gcagttggcg
     7861 tggtggcact ggtgctttat ctgctgcacg ccagaaataa caaccgcgcc ctgttcagtc
     7921 tgaaactgtt ccgcactcgt accttttcgc tgggcctggc ggggagcttt gccggacgta
     7981 ttggcagtgg catgttgccc tttatgacac cggttttcct gcaaattggc ctcggtttct
     8041 cgccgtttca tgccggactg atgatgatcc cgatggtgct tggcagcatg ggaatgaagc
     8101 gaattgtggt acaggtggtg aatcgctttg gttatcgtcg ggtactggta gcgaccacgc
     8161 tgggtctgtc gctggtcacc ctgttgttta tgactaccgc tctgttgggc tggtactacg
     8221 ttttgccgtt cgtcctgttt ttacaaggga tggtcaactc gacccgtttc tcctccatga
     8281 acaccctgac gctgaaagat ctcccggaca atctggcgag cagcggaaac agcctgctgt
     8341 cgatgattat gcaattgtcg atgagtatcg gcgtcaccat cgccgggctg ttgctggggc
     8401 tttttggttc gcagcatgtc agcgtcgaca gcggcaccac acaaaccgtc tttatgtaca
     8461 cctggcttag catggcgttt atcatcgccc ttccggcgtt catctttgcc agagtgccga
     8521 acgatacgca tcaaaatgta gctatttcgc ggcgaaaaag gagcgcgcaa tgaagttctg
     8581 gcgacccggt attaccggca aactgtttct ggcgattttc gccacctgca ttgtcttgct
     8641 gatcagtatg cactgggcgg tgcgtatcag ttttgagcgt ggctttattg attacatcaa
     8701 acacggcaat gaacagcgat tacagctgtt aagtgatgcg cttggcgagc agtatgcgca
     8761 gcatggtaac tggcgcttcc tgcgcaacaa cgatcgcttt gtctttcaga tcctgcgttc
     8821 atttgaacac gataattcgg aagataaacc cggcccgggt atgccgccgc acggctggcg
     8881 tacccagttc tgggtggtcg atcaaaacaa caaagtactg gttggtccgc gagcgccgat
     8941 tccacctgac ggtacacggc gacccattct ggtcaacggt gcggaagtgg gcgcggtgat
     9001 tgcctcccct gttgagcggc tgacccgcaa tactgatatc aatttcgata aacaacagcg
     9061 gcaaaccagc tggctgatcg tcgccctggc aacgttactc gcggcgctcg ccacctttct
     9121 gctggcgcgc ggtttgctgg caccggtaaa acgacttatc gatggcacgc acaaactggc
     9181 ggcgggcgat ttcactaccc gcgtaacgcc caccagtgaa gatgaactgg gcaaactggc
     9241 gcaagacttc aaccagctcg ccagcacgct ggagaaaaac cagcaaatgc ggcgcgattt
     9301 tatggccgat atttctcacg aactgcgtac gccattagcg gtgctgcgcg gtgaactgga
     9361 agccattcag gatggcgtgc gcaaattcac gccggagacg gtggcgtctt tacaggcgga
     9421 ggtcggtaca ctgaccaaac tggttgacga tcttcatcag ttgtcgatgt ctgatgaagg
     9481 cgctctcgcc tatcaaaaag caccggtaga tttgatccca ctgctggaag tggcgggcgg
     9541 cgcatttcgc gaacgattcg ccagccgtgg cctgaaactg caattttccc tgccagacag
     9601 tatcaccgta tttggcgatc gcgaccgttt aatgcagtta ttcaataact tactggaaaa
     9661 cagcctgcgc tacactgaca gcggcggcag cctgaaaatc tctgccgagc agcacgacaa
     9721 aacggtgcgc ctgacctttg ccgacagtgc gccaggtgtc agtgacgatc agctacaaaa
     9781 attgtttgaa cgtttttatc gcaccgaagg ttcccgcaac cgcgccagcg gcggttccgg
     9841 gctggggctg gcgatttgcc tgaacattgt tgaagcccat aatggccgca ttattgccgc
     9901 ccattcgcct tttggcgggg taagcattac agtagagtta ccgctggaac gggatttaca
     9961 gagagaagta tgaccgagtt accaatcgac gaaaacacac cgcgtatttt gatcgtggaa
    10021 gatgaaccga agctggggca gttgctcatt gattatctgc gtgctgcgag ctatgcgccg
    10081 acgcttatca gccacggcga tcaggtactg gcgtatgtgc gccagacacc accggatctg
    10141 atcctgttgg atctgatgct ccctggcacc gatggcctga cattatgccg ggaaattcgt
    10201 cgtttttccg atgttccgat cgtgatggtg acggcaaaaa tcgaagagat cgaccgcctg
    10261 ctggggctgg agattggcgc cgatgactac atctgcaagc cgtacagccc acgggaagtg
    10321 gtagcgcgtg tcaaaaccat tttgcgccgt tgtaaaccgc aacgcgaact tcagcaacag
    10381 gatgccgaaa gcccgttgat tatcgacgaa ggtcgttttc aggcttcatg gcgcggtaaa
    10441 atgcttgacc tgacgcctgc ggaatttcgt ctgcttaaaa cgctctctca cgaaccagga
    10501 aaagtgttct cccgcgagca attgctcaat catctttatg acgactaccg cgtagtaacc
    10561 gaccgcacca ttgacagcca cattaaaaat ctgcgccgca agctggaatc tctcgacgcc
    10621 gaacagtcat ttatccgcgc cgtttacggc gttggttacc gctgggaagc cgacgcctgc
    10681 cgcatcgttt agttttagcg acattacttt gttagccgga gatggcgttc agacccgatc
    10741 cggcaacatt attccacgca tggtcagcaa actgacatta tctctccgcc ctggcaggca
    10801 tcagaaaatc gctcatactt taatcagtaa acagcacctt tagatgctgt tttcgataag
    10861 caatttcaat caaggagtca ttatggctgg ttggtttgaa ctcagtaaga gcagtgataa
    10921 tcagttccgg tttgtgctaa aagcgggcaa tggtgagact atcctcacca gcgagctgta
    10981 tacctcaaaa gcctctgcgg aaaagggcat cgcgtcggtg cgtagcaaca gcccgcaaga
    11041 agagcgctat gagaaaaaaa cggcaagcaa cggcaaattc tatttcaatc tgaaagccgc
    11101 taatcatcaa attatcggct ccagccagat gtacgccacc gcgcaatctc gtgaaaccgg
    11161 aattgcctcc gttaaagcca atggcacaag ccagacggtg aaagacaata cgtaattacg
    11221 atgccgggcg tgggttgttg gcgtccggcg ctttaccaga tcccccctac agcgctacaa
    11281 tgcccgccct taaagtgggg gcctccccta accgcttcat caggtgaagc ggatctgacc
    11341 tgtcatcaga acgagagaat tatgtttaaa ccggaactcc tttccccggc gggaacgctg
    11401 aaaaatatgc gttacgcttt cgcttatggc gcagatgctg tttatgcggg ccagccgcgt
    11461 tactccctac gtgtgcgcaa caacgaattc aaccacgaaa atcttcagct cggcatcaat
    11521 gaagcccacg cgctggggaa aaagttttat gtcgtggtca acattgcacc acacaacgcc
    11581 aagctgaaaa cctttatccg tgacctgaaa ccggtggtgg aaatggggcc ggatgcgctg
    11641 attatgtccg atccagggct gattatgctg gtgcgtgagc acttcccaga aatgccgatc
    11701 cacctctcgg tgcaggctaa cgccgtgaac tgggcgacgg tgaaattctg gcagcaaatg
    11761 ggactgaccc gcgtgatcct ctctcgcgag ctgtcgctgg aagagattga agagatccat
    11821 aatcaggtgc cggatatgga gatcgagatc ttcgttcacg gcgcactgtg catggcctac
    11881 tccggtcgct gcctgctctc tggctatatc aacaagcgcg acccgaacca gggcacctgc
    11941 accaacgcct gccgctggga gtacaacgtc caggaaggga aagaagatga cgttggcaac
    12001 atcgtacaca agtacgagcc gattccggtg caaaatgttg agccgacgct gggtatcggc
    12061 gcgccaaccg acaaagtgtt tatgatcgaa gaagcccagc gtccgggcga gtatatgacc
    12121 gcgtttgaag atgagcacgg cacttacatc atgaactcga aagatctgcg cgccatcgcc
    12181 catgtagaac gcctgaccaa aatgggcgtg cattcgctga aaatcgaagg tcgtaccaaa
    12241 tctttctact attgcgcacg caccgcacag gtttaccgca aagctatcga tgacgccgct
    12301 gcgggaaaac cgttcgatac cagcctgctg gaaactctgg aaggtctggc gcatcgtggc
    12361 tataccgaag gtttcctgcg tcgtcatact cacgacgatt atcagaacta cgaatacggt
    12421 tattcagttt ctgaccgcca gcagtttgtt ggtgagttta ccggtgagcg caagggggac
    12481 ctcgcagcgg tagcggtgaa aaataaattc tccgttggcg acagtcttga gctgatgacg
    12541 ccgcaaggca acattaactt tacccttgag cacatggaaa acgccaaagg cgaagctatg
    12601 ccggtcgcac caggcgatgg ttatactgtg tggctcccgg tcccgcagga tcttgagcta
    12661 aattacgcgc tgctaatgcg taatttctcc ggggaaacca cgcgtaaccc ccacggtaag
    12721 tgattaattt cgattatttt tcccggatgg aaaattctta gaaaccgatc acatacagct
    12781 gcatttatta aggttatcat ccgtttcgct gaaaaacata atccataaaa tgctagctgt
    12841 accaggaacc acctccttag cctgtgtaat ctcccttaca cgggcttatt ttttgtacat
    12901 aagctgtaaa gaaggattga tccctgataa ccgtcttcca ttgaccacat cgatagaatc
    12961 ccccttcata gcacaatgcc tttcacgtaa cggtatcgtg ctcgcccagg ttccggctac
    13021 gcacagccag aacgcgcatg tttgacgctt accaaaaaat attctcactc tccacatttg
    13081 aatgtcagac gagcgacgcc atgtaatcct gcaccttctg tcttcaggtc aactatctgc
    13141 atttttttgc cctgagtaac acagaaatgg gctgcatcat tttttactat attttctgca
    13201 ccagatattc tgcccctggc taaagaagct tcggcttcgg tgtagtattg gttatcgagt
    13261 ttacgctgaa tattactttt atatgcaaga ccaaatttac cgatacttgt ttcatcatta
    13321 tgcacagcac aaccagatat aagaaaaata ctaattaatg atatagcagc tatctttttc
    13381 atctcacctt cccccattaa ataccaacga cactctctag tgtttaaata taataatggc
    13441 atgattatta taattgaata ggattataat aaatgttctg tacaacattt cctacataag
    13501 taggaattac ggacattgag gcccttcagg gtaactccat gggggcttta atatattata
    13561 ttgaagatgc cactgtttag ttgaatatta ggtatatgct cttttttgaa atttataggt
    13621 ggcagccgtt agtattcgct gtccccattg caagctcctg gtggtaacca ctgaatcctc
    13681 catacttgaa ctgacttttt atcctccgac tttcatcctg ttctgactcc accttttgtt
    13741 ttctgctcta cactatctac agaccaatca taaaggcaca tacgatcatg gcagaatttc
    13801 ccgccagctt actgattctt aatggcaaaa gtactgacaa tctacccttg cgcgaagcaa
    13861 ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg ggtcacctgg gagaaaggtg
    13921 atgccgcacg atttgtagag gaggcccgga agttgggcgt cgcaacggtg attgccggtg
    13981 gtggcgatgg caccattaat gaagtttcta cggcgttgat tcagtgtgag ggggatgaca
    14041 tacccgcgct gggaattttg ccattaggaa ccgccaatga ttttgccacc agtgtaggga
    14101 ttcctgaggc actggataag gcgctgaaac tggcaattgc cggtaatgcc attgcgatag
    14161 atatggcgca ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc ggatttggga
    14221 cgcgtattac cacagaaacg ccggaaaaat taaaagccgc gctgggtggc gtctcttaca
    14281 tcattcatgg cttaatgcgc atggatactc tgcaaccgga ccgttgtgaa atccgcggtg
    14341 aaaactttca ctggcaaggt gacgccctgg tcattggtat tggtaacggg cgtcaggccg
    14401 gtggcggtca acaattgtgc ccgaacgcgt taattaacga tggcttgctg caactgcgca
    14461 tttttaccgg cgatgaaatt cttccggctc tcgtatcaac cttaaaatct gacgaagata
    14521 acccgaatat tatcgaaggc gcttcgtcgt ggtttgatat acaagcccca cacgaaatca
    14581 cttttaatct tgatggcgaa ccgttgagtg ggcaaaactt ccatattgaa atacttccgg
    14641 cggcgttgcg ttgtcgatta ccaccagatt gtccattatt gcgttaatca aactattctc
    14701 tcttgaacca gggagaatcc gtaatgccgg atgcggtgta aacaccttat ccggcataca
    14761 gaacaatact acaccgtaat taattttacc cgtgtcttct gcaatgccaa tttatccacc
    14821 tctgacacag catcatcggt aacaataatg tcaatttttt cgattggcag tacctgatta
    14881 aaaccgcgac gattaaattt cgatgaatcg agaacggcaa tcactttatt tgcggcggtg
    14941 accatcgccc cgctaatcga ataaccttca ttaaaggtcg taataccatt aaccgcatca
    15001 ataccatcag caccgacaaa cattaaatcg gcattaatat cctgcaatga acgctcggca
    15061 atggaaccat gcatcgagcg cgttttatgg cggaccgtgc caccacagac gaccagagta
    15121 atgtctttat tttcggaaag ggcaaacgct gccgggagac tgttggtaat caccgtgata
    15181 tttttagcgg tcattaatcc ttcggcgata agcattgtgg tacttccgct gtcgagaata
    15241 acggtcatcc cttcatggat cattttgact gccgcctgcg ctattctgtt ttttggcgcg
    15301 ctggcgagct gaaatcgctc tttaaacccg acttcctggg tctcggtttc actattaccg
    15361 gacattattt tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg ttcgagaaag
    15421 cgcaaatcgg cacggattgt cgcttccgag gcagcaaata ctcccgccag atcctgaaca
    15481 agcacggttc cctgttcatt cactaattgg atgatcttat tccttcgctc gaatgaattc
    15541 atcgtcattt acccatattc aattgtggct agtgtaaacg aagggggacc attggtgaat
    15601 gatctgtttt gcacaatacc gggccagcat cacgctggcc cgcggtttca tggaatgagc
    15661 aacactttgc ccggcatggc attacgagcg atgtcacgca ccgcctgggt gaagctttca
    15721 aagcttccac ggtgagcgat taatggatcc aggcttaact tacgttctgt cagcaaccgg
    15781 ctcgccgttt cccactcctg ccccggccaa gggctggaat agttcatcca actgccgata
    15841 accgtcagct ctttacgcaa tattttgcca aacgttgccg atgttaaatg cagatcctga
    15901 tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg caatctctac cgccagttcg
    15961 acagtttgcg gtacgccagc cgtctcgagg ataagctgat taaagcgcag ttcgcgtaaa
    16021 acgctctgca tttgcggcgc gctcatttcg ctactgttaa atgtttgcat cgcaccgaaa
    16081 gattttgcca gtgccagttt ttctgagcta atgtcgatcg ccgtcacact ctttgctccc
    16141 agcgcgacag cgcactgaat agccagcagg ccaatggttc cggcaccaat aataataacg
    16201 tttttattct cacaaccttg cgctaaatga aaagcatgca ggccaacggt aatcggctca
    16261 ataaaagccc catcctcaat aggcatatcc gtgggtagag caaagacatt ttttcgctta
    16321 acgacaatat attcagcaaa tccaccatca cgccgcgagc caataaaatc atattttgcg
    16381 cactgggaat aaaacccttt caaacactct ggacaagtaa aacagggtaa taacggcaca
    16441 caggcaaccg catcgccagg gtgtaaatca tcaacaccgg atcccaccgc atcaatatag
    16501 ccgctaaatt catggcctaa cgttattgga taataatgtg caccattttt aaatatcctg
    16561 ggtaaatcgg aaccacataa gcccgagctg gcaattttta cccgcacctc atcctgat
//
LOCUS       DAJTNM010000081        16388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTNM010000081 DAJTNM010000000
VERSION     DAJTNM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.081"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..584)
                     /gene="kptA"
                     /locus_tag="OO172_002745"
     CDS             complement(<1..584)
                     /gene="kptA"
                     /locus_tag="OO172_002745"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5512873.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            833..1516
                     /locus_tag="OO172_002746"
     CDS             833..1516
                     /locus_tag="OO172_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5512874.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1513..1974
                     /locus_tag="OO172_002747"
     CDS             1513..1974
                     /locus_tag="OO172_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5512875.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            1987..3159
                     /gene="iadA"
                     /locus_tag="OO172_002748"
     CDS             1987..3159
                     /gene="iadA"
                     /locus_tag="OO172_002748"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5512876.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTLFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3224..4135
                     /gene="hypT"
                     /locus_tag="OO172_002749"
                     /gene_synonym="qseD"
     CDS             3224..4135
                     /gene="hypT"
                     /locus_tag="OO172_002749"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5512877.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4128..4520)
                     /gene="iraD"
                     /locus_tag="OO172_002750"
     CDS             complement(4128..4520)
                     /gene="iraD"
                     /locus_tag="OO172_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5512878.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4517..4600)
                     /gene="idlP"
                     /locus_tag="OO172_002751"
     CDS             complement(4517..4600)
                     /gene="idlP"
                     /locus_tag="OO172_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5512879.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5193..6023
                     /locus_tag="OO172_002752"
     CDS             5193..6023
                     /locus_tag="OO172_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5512880.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(6164..6937)
                     /gene="uxuR"
                     /locus_tag="OO172_002753"
     CDS             complement(6164..6937)
                     /gene="uxuR"
                     /locus_tag="OO172_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5512881.1"
                     /translation="MKSVTSVQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7152..8612)
                     /locus_tag="OO172_002754"
     CDS             complement(7152..8612)
                     /locus_tag="OO172_002754"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5512882.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLTLGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8693..9877)
                     /gene="uxuA"
                     /locus_tag="OO172_002755"
     CDS             complement(8693..9877)
                     /gene="uxuA"
                     /locus_tag="OO172_002755"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5512883.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10217..11560
                     /gene="gntP"
                     /locus_tag="OO172_002756"
     CDS             10217..11560
                     /gene="gntP"
                     /locus_tag="OO172_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5512884.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11811..12713)
                     /gene="fimH"
                     /locus_tag="OO172_002757"
     CDS             complement(11811..12713)
                     /gene="fimH"
                     /locus_tag="OO172_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5512885.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12733..13236)
                     /gene="fimG"
                     /locus_tag="OO172_002758"
     CDS             complement(12733..13236)
                     /gene="fimG"
                     /locus_tag="OO172_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5512886.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13249..13768)
                     /gene="fimF"
                     /locus_tag="OO172_002759"
                     /pseudo
     CDS             complement(13249..13768)
                     /gene="fimF"
                     /locus_tag="OO172_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
     gene            complement(13778..>16388)
                     /gene="fimD"
                     /locus_tag="OO172_002760"
     CDS             complement(13778..>16388)
                     /gene="fimD"
                     /locus_tag="OO172_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5512887.1"
                     /translation="QRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSSADLYFNLRF
                     LADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTR
                     AQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTIPQAFMSNRA
                     RGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTT
                     WSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLAS
                     DDNMLPDSQRGFAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGD
                     LQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSIMAGEYRSGNAQQEKPRFFQSTLL
                     HGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSV
                     RFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTD
                     YYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINW
                     TLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTN
                     LAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIK
                     QLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPY
                     ATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNK
                     PLPFGAMVTSESSQSSGIVADNGQFYLSGMPLAGKVQVKWGEEENAHCVANYQLPPES
                     QQQLLTQLSAECR"
BASE COUNT         3972 a         4221 c         4013 g         4182 t
ORIGIN      
        1 tattgttttt gtaccccagt cggcataaac gaccactcaa gaaactcaac agcaaccgtt
       61 gatgtcagcc agaccccgtt ttccgcttgc cagaaaggga tgcctcgttt cgccatttcc
      121 tgtgctttga cggttaagac aaccggcgat ccgtgtcttg ctcccacttt gcgtgccgtt
      181 gcttcatcag cagagagatg cacgtaatga cgatcccctg caatcagccc ctgttttttt
      241 atctcgtcaa ggaaacggct tgccgtaccg tggtagagaa actgtggcgg tgttttttcg
      301 gcaaaggaaa tagcgacctg cgaagtcgaa tgcccctgaa ccgcgcggat gcacctacca
      361 tcactggaat aactaaaacg ctttttatcg ctggttgcga ccacggtatc cagcaatgca
      421 cgggttaatc gcttgcccgc tttttgggca caaagtatga gcttattgat atccgcccat
      481 ccttcacggt ccagtacgat accaatcgcc tccggtttgt gtcggaggac aaaacttaaa
      541 aacttgctcg tatccgccag ctctttttcg ttgtactttg ccatcgcgtt ttcgtccctg
      601 gattttctgt ttttatttac taatgtcatt gtcactgggt ttatgaattt tctgcaactc
      661 aatcctgcgc agaacctttt ccacacagcg taatttttcc cctatgcaaa aaacgcatag
      721 ccagaaacat acttgcattt tccccatacc cacatggctg ataaggtttt tcagttccag
      781 gaattttcct ggctcaagca acacacaaac acaaaaaaca atggggattg ttatgacaca
      841 acaaggggat gctgtagccg gggagctggc gacagaaaaa gtgggcatta aagggtatct
      901 ggcttttttt ctcaccataa tctttttttc cggtgttttt tccggaacag acagctggtg
      961 gcgcgttttt gatttttccg ttctgaacgg atcgtttggg caactgcctg gtgcgaatgg
     1021 agccaccacc tcatttcgcg gggtgggtgg cgcgggggct aaagatggct ttctctttgc
     1081 gctggagctg gcaccgtcag tcattctctc tctggggatt atttcaatta ccgatggtct
     1141 tggtggatta cgcgccgcgc agcagttaat gacacctgtg ctaaaaccgt tgttgggcat
     1201 tccagggatc tgctcgctgg cactgatcgc taacctgcaa aacacggatg ccgccgccgg
     1261 tatgaccaaa gagctggcgc aggaaggtga aattaccgaa cgcgataaag tgattttcgc
     1321 cgcatatcaa accagcggta gcgccatcat caccaactac ttctcttccg gcgtcgcggt
     1381 gtttgccttt ctgggcacgt ccgtaatcgt acctttagcc gtcatcctgg tgtttaaatt
     1441 tgtcggagcc aacattttgc gcgtctggct taacttcgaa gaacgtcgca acccaacgca
     1501 aggagcgcaa gcatgacaac tcaggtccgc aaaaacgtca tggatatgtt tatcgatggc
     1561 gcgcgccgtg gctttactat cgccacgacc aacctgctgc caaacgtggt gatggcgttt
     1621 gtcatcattc aggcgctgaa aattaccggc ctgctcgact gggtggggca tatctgtgaa
     1681 cccgtaatgg cgctgtgggg attacccggt gaagcggcaa ccgtcctttt agccgcatta
     1741 atgagtatgg gcggcgcagt cggtgttgcc gccagtctgg caaccgctga cgcattaacc
     1801 ggccacgatg tcaccgtttt actgcccgca atgtatttaa tgggcaaccc ggtgcaaaat
     1861 gtcggtcgct gcctggggac agccgaagtg aatgccaaat attacccgca tatcattacg
     1921 gtctgcgtga ttaacgcatt gctgtcgatc tgggttatgc agcttattgt ttaacaagga
     1981 gttaccatga ttgattatac cgcagccggt tttaccctgc tgcagggagc gcatttgtat
     2041 gcgccggaag atcggggaat ttgcgatgtc ctcgtcgcta acggcaaaat tatcgccgtt
     2101 gccagcaata tcccttctga cattgtaccg aactgcacgg ttgtcgatct cagcgggcag
     2161 atcctctgcc caggttttat tgatcaacac gtccatttga ttggcggtgg cggcgaagca
     2221 ggtcccacga cgcgcacgcc ggaagtggcg ctaagtcgcc tgacggaagc gggcgtcacg
     2281 tcagtggttg gtctgctggg caccgactct atctctcgcc acctggaatc cctgctcgcc
     2341 aagacccgtg cgctcaatga agaaggcatc agcgcctgga tgctgaccgg cgcttatcat
     2401 gtcccttccc gcactattac gggttccgtg gaaaaagacg tggcgattat cgatcgtgtg
     2461 attggcgtga aatgcgccat ctctgatcac cgttctgccg caccggacgt ttatcacctg
     2521 gctaatatgg cggcagaatc ccgcgttggc ggtttgctcg gcggtaaacc tggcgtcacc
     2581 ctgttccaca tgggcgacag taaaaaggcg ttaaagccta tttatgacct gctggaaaac
     2641 tgcgatgtgc cgatcagcaa gctgctgccg acccacgtta accgcaacgt accgttgttt
     2701 gagcaggcgc tggagttcgc gcgcaaaggc ggcaccatcg atatcaccag cagcattgac
     2761 gaaccggtcg cccctgccga aggtattgcc cgcgccgttc aggcgggtat tctgctggca
     2821 cgcgtcaccc tcagctccga cggcaacggt agccagccgt tcttcgatga cgaagggaat
     2881 ttaacccata tcggtgttgc aggttttgaa acgttgctgg aaaccgtgca ggtgctggtc
     2941 aaagactatg atttcagtat cagcgatgcc ctgcgcccgc tcaccagtag cgtagccggt
     3001 ttccttaaca tgagcgggaa aggcgaaatt ctgccaggca atgacgcaga cttactggtc
     3061 atgacgccag aattgcgcat tgagcaggta tacgctcgcg gcaaactgat ggtcaaagac
     3121 ggcaaagcct gcgtgaaagg aacgtttgaa acggcttaaa cggttgtcag ccgccctaat
     3181 ggacgtagaa tgcccccaag ggcggctgac agagtaaaac gtaatggatg actgtggtgc
     3241 ggttttgcat aatattgaaa ccaaatggct ttatgatttt ctgactctgg aaaaatgccg
     3301 caatttttcc caggcggcag tcagtcgcaa cgtctcgcaa ccggcattca gccgccgcat
     3361 ccgtgcactg gaacaggcga ttggcgttga attgtttaac cgccaggtga cgccactgca
     3421 actctcagaa caagggaaaa tcttccattc gcagatccgc catctgttgc aacagttaga
     3481 aagcaacctt gcagagctgc gtggcggcag cgattacgcg caacgtaaaa tcaagatagc
     3541 cgctgcacac tctctttccc tcggtctgtt accgtccatt atcagccaga tgccgccgct
     3601 ctttacttgg gcaattgaag ccattgatgt cgatgaagcg gtcgataaac tgcgtgaagg
     3661 gcaaagtgac tgtatttttt cctttcacga cgaagatttg ctggaagcgc cgtttgacca
     3721 cattcgctta tttgaatctc aattgttccc tgtctgcgcc agtgacgaac acggagaagc
     3781 actttttaac ctcgcgcagc cacactttcc gttactgaat tacagccgca actcctacat
     3841 ggggcgattg attaatcgca ccctgacgcg ccacagtgag ttaagtttca gcgccttttt
     3901 tgtctcttcg atgagcgagc ttttaaagca ggttgccctc gacggctgtg ggattgcctg
     3961 gctgccggag tatgccatcc aacaagaaat tcgcagcggg cagctcgttg tgctgaatcg
     4021 ggacgaacag gtgatcccga ttcaggccta cgcataccgg atgaataccc gcatgaatcc
     4081 agttgccgaa cgcttctggc gtgaactgcg cgagctggag attgtgctta gctgacattc
     4141 tcaagcgtcg cactgcgcca accgccatta tcccagacca gatcaaacgt tagcggttca
     4201 tcgtcgtggt acagcattat ctcaaggctg atcgccagag tgtggcagta cgcttctttc
     4261 aaattaacct gcaacgccgc aatccgtggc tcatgaaata acagtgcaga acgcagctga
     4321 cggcaatacc agctctggag atgtttgtcg ctcgggttat gcacttgcca ctcgggcatg
     4381 cctgaaacaa gtaatgacgg taatgccgaa tgaggagaat taaaaagagt aaggagatcg
     4441 ctgcataccg atttacgcag caatgacgtt ttattgcctg aaatttcagg taaaacagcc
     4501 tgaagtgatt ggcgcatcat tttgcgcact cctgacgttt agcaacatat gcggcttgcc
     4561 ctgaacaccg ggcaccactg tattgatgct cattttccat agccattccc ttttgttcat
     4621 tacatccatg tcagtctgag acaggcctga atcctgtcag caaagtataa aaccttactt
     4681 gcctatgaat atctactgac ttttcaggaa aaaagtcatg atttgataaa aatcatcaaa
     4741 ctcaaaaaaa aaccgctagt atattctgaa tattccgaat agttacgtag atgaaaatac
     4801 ttgtcaccgc tgctacttaa ttccggtggg aaattattta tttccgcata atcagccatg
     4861 tattatttga aacaattagt ttgtaaataa tataaattga taacatatca aatacatcaa
     4921 aaataccatt cacaggcatt attaatatag acctcaatat agcaacatca aattccaatt
     4981 agtaataaga tatgctttta aattgcatgc aaataatttt aaatgagcgt tttttgatac
     5041 tttatagtgt ttcctaccct ttaatatcac tgtgtagagt tgatatcact acaacagaaa
     5101 catgcatgtc atttgtttac ttctcttttt actacagaaa aatttatcta tttttctaaa
     5161 catcagttaa atactcataa aaggtaacta ttatgtctat gccattaagc aatgccgcta
     5221 ataaccaatt caatacaaat aatcacttcc ttcatcatcc aaaagtcgac agtgagttaa
     5281 cacgtcgata tgaatatgca cgcctggata cggaaaatat ctatcttctg cctcttgctc
     5341 gtggcaataa ccataattac gatggaaaaa gcgttgtcga aattagaaaa cttaatattg
     5401 gaaaacagtc ctggccattt aattatgtga cagatacatg cagagaattc aatggtatta
     5461 caacaacagg acgtatgctc tatcgaaact tgaaaatcac ctcagcttta gacgaaatat
     5521 atggtggtat atgtaaaaag gcgcatgctg ctacagagtt agcagaaggt ttacggctaa
     5581 atttatttat gaagagccct ttcgatcctg ttgaggacta tacagttcat gaaataacat
     5641 taggccctgg ttgcaatgtt cctggttatg ccggaaccac gataggatat atttcaaccc
     5701 ttccagcttc tcaggcaaaa agatggacca atgagcagcc aagaattgat atctatattg
     5761 atcagataat aactgtctca ggggtagcaa attcttccgg attcgccctt gccgcattac
     5821 ttaatgctaa tattgagatg ggaaatgacc caatcattgg tatagaagcc tatcctggta
     5881 ccgcggagat ccattcaaaa atgggatata acgttatccc cggtgatgaa gatgcgccac
     5941 tcaaacgcat gactctgcaa ccgtcatctc ttccagaact gtttgaatta aaaaatggtg
     6001 aatggaatta tattggtaaa tagccactga gaagaatgag tactaatatt caggttatac
     6061 agatatttaa acaaaaccct gcaatttaca aagtaatact cattgcgact tcttaattta
     6121 agaaagcgca aggaacgttt acccttgcgc ttattataat aagtcaggcg tcgactttat
     6181 ccagcggcca tgaatcaaag agatagccgt caaaataaat atcgtcaacg ttcgagaatt
     6241 ccagcagacg ttgcttaacg ttttccagat gctgccacat tgccagcttc gccgctcggg
     6301 catctttttt gattaacgcg gcgaggatct gtttgtgatc gcccaaccac tctttgcgat
     6361 acaggctgtc atccagatgg ctgtgcaact ggatccacat tggattgttt tcccgccact
     6421 gccaggactg acggaataac tccaccagca tgctgttatg cgttgcttca gcaatggcga
     6481 gatggaactg catgtcaccg ctttcgctgc tacccggcgc gctggaagcc agttcacgct
     6541 cttccagttg caatgcctga cgcattttga cgatatcttc gcgggtagcc tgcagtgcgg
     6601 caaactcggc gatgttactt tccagtaact gacgcgcctg taacagctca aaaggacccg
     6661 catcattgca gacgttggca tccggactgt ctgtgttgtg gctgcctgag ctatcaagca
     6721 catagatacc ggcaccccgg cgcacttcca ccagcccttt gatctccagc atgatcagcg
     6781 cttcacgtac caccgtccgc gtgacatcaa gcatttctgc aatttcacgc tccggcggca
     6841 gccgttcgcc aggattgtac ggcgtcttta tgatcagatc gcggatcatc gccccgactt
     6901 cctggtaagg tctttgcaca gaggtgacag atttcatggt gttgtgtccg ttaggtgcgg
     6961 ttaatcaaat ttttgccgga tgcggcgtga acgccttatc cggcctacaa cgggcaattt
     7021 tacggccccc gttaatctca aactatacca ttagcactgg aagttggcac gtacaggtgg
     7081 gcaaaaaaac gaaaactggt caaccaaata tcagtttgat tgaccagtaa gtctgtaggg
     7141 taatcagtta gttacagcgc agccacacac tcgcgcgcac cgcgttcgca cagctgctga
     7201 tatgccgccg tcaccgcgcc aacaaagtcg gcattctgcg gcagatcatc ggcaaaaata
     7261 ccgctcaggc ccagcagcgc tttcacgcgg tctgcgccct gatactgcgc gttaatcttc
     7321 tggaactcgg ccagcatcgg gtcgaccacg tcaatggcat taccctgctc atccacgccc
     7381 tgggtgtaac gcatccagcc agccacgccc agcgtcaggt gacgccagct gccgccgttt
     7441 tgcaggtgca gacgcaccgg gtccagtaga cgctgcggta acttctggct gccgtccatc
     7501 gcaatctgcc aggtacggtg acgcagagac gggttgctga aacgctcgat cagcagcgtc
     7561 gcataggcgt tcaggtccgt accttccggc atcgacagcg ttggcgcttg ttcctgcatc
     7621 atcagggcaa aggccgcttt gcgataatcc gggttagtca tggtgtcggc aatggtttca
     7681 tagccgccga ggtaaccgag gtacgccagg aaagagtggc tgccgttcag catacgcagc
     7741 ttcatcattt cgaacggcac aacgtctgca acgaactgtg cgcccacttt atcccagtcc
     7801 gggcgaccat taacgaagtt atcttcaatc acccactgac ggaacggttc gcaggcaatg
     7861 gcgcatgggt cgtaaacacc cagctggtca gcaatttcct gtaaggtttc tggcgtcgcc
     7921 gccggaacga tgcggtcaac catggtgcac gggaaggtga cgttctcttc aatccatgcc
     7981 gctagttgcg ggtcacgcgc ctgagccagc cccagtaccg cgaccttcgc cacatgaccg
     8041 ttttcacgca cgttatcgca ggacatcacc gtaaatgctt tcagcccttt ttcacgacgc
     8101 agacgcaagg cttcgacgat gtaaccaatc gcggacttcg gcgcagtcgg gttttccaga
     8161 tcgtgcttga tcagcgggtt attgagatcc agttgaccgc ttgccgcatc agcgcagtag
     8221 cctttttccg tgaccgttag agagacaatc gccgtttgcg gacgcgccat cgcgttgaga
     8281 ataccttcgc agccgtcgat ttccggatgc agcgcttctt tcatcgaacc gataattttc
     8341 agctcggtgc tttctgcgcc tttttccgct acggtgtaca gcagttgctg ttttttcagg
     8401 ttttcgatca gcatacggtc gttgcctggc atcaggttaa cttcgcagat gccccagtcg
     8461 ctgtcggtgc tttccagcag atgatgggta tacaacgcct ggtgcgcgcg gtgaaacgcc
     8521 ccgcaaccga gatgcacaat gcgtgattcc agacgagaat gatcccatga cgggcgggca
     8581 accggcagat tgctgtcaac aatagtagtc atcgtaaact ccagaatccg tcttcagaga
     8641 gtgacggcaa ggaagaagga accggcacgg cgcgcagcca tgccggtggg tattaacggc
     8701 taaagaaagc gcgctggatc gccagttcga caccgcgaac ttcggccagg cctttcagac
     8761 gaccaattgc ggagtaacca gggttggttt tcttcttcag gtcgtccagc atctgatgac
     8821 cgtggtccgg acgcatcggg atcaggtctt ctttgccttc cgctttacga cggtgttctt
     8881 cttcaacaat cgctttcacc acttcgtaca tatcaacgtc accgttcagg tgcgccgctt
     8941 cgtggaaggt tttcgggtta tcttcacgca tggtggagcg cagatgggtg aagtaaatac
     9001 gcggaccgaa ctgcttgatc atatcaacca gatcgttgtc agcacgcacg ccgtaggaac
     9061 cggtgcacat ggtgaaaccg ttcgccatgc tgtttacggt atcaaccatc cactgcatat
     9121 cttcaatggt ggaaacaatg cgcggcaagc cgaggatcgg gcgcggcgga tcgtccgggt
     9181 gaaccgccat acgcacgcca acttcttcag caactggaat aatcgctttc aggaagacgg
     9241 caaagttttc gcgcagtttg gctttgtcga tatctttgta cagctccagg tgcttacgga
     9301 actggtcgag ggtataccct tcttccgcgc ccggcagacc ggcaatgatg ttacgcgtca
     9361 gacgcgcttt gtcttcgtcg ctcatagtgg cgaagcgttc agctgcctga gcaatttctt
     9421 cttcggtgta atccgcttcc gcgcctggac gtttcaggat atgcatttcg aatgcagcga
     9481 attcgatctg gtcgaagcgc agagctttgg agccgtctgg cagcacgtat tcgaggtcag
     9541 tacgggtcca gtcgagcacc ggcatgaagt tgtagcacac ggtgcgaatg ccacactgcg
     9601 ccaggttgcg cagagtctgc tgatagttag cgatccactg ctcatagttg ccagtgtggg
     9661 ttttgatatc ttcgtgaatt ggtacgcttt ctacgacaga ccacaccagg cctgcgtctt
     9721 caacgatcgc cttgcgtttg aggatctctt ctacggacca tacttcgccg ttcgggatat
     9781 ggtgcagcgc ggtaaccacg ccagttgcgc ccgcctgacg gacatcagct aaagaaaccg
     9841 gatcgtttgg gccgtaccag cgccaggtct gttccatgat ttttcctctt tcacatagtg
     9901 ggttccacag caaagagacg cggaaccttg gagttgttta ggtatccgga tgaacaacca
     9961 gaccgcagcc gtggcgttgc atcggatgag atggcgtata agttctacct tttggtaaga
    10021 catgtcaacc cgaactctga aattggttaa ccacatcaca agaatttcac tctcatggtt
    10081 gttgctgcaa attgcagcaa agggtaatcg atggttattg atcagaggat gtgacattca
    10141 tcgcaacaat ggttgaccaa tttgcatgac atgtcgccca aaataacaac ggttcaacca
    10201 agtaaggtaa aacgttatgc atgtgcttaa cattctctgg gtggtattcg gcattggcct
    10261 gatgctgata ctgaatttga agttcaaaat caattcaatg gtggctttgt tggtggcggc
    10321 gctgtccgtc gggatgctgg caggcatgga tttgatgtcg ctgctgcaca ccatgaaagc
    10381 gggcttcggc aacacgctgg gagaactggc tatcatcgtg gtgttcggtg cggtcatcgg
    10441 taaattgatg gtcgactccg gcgcggctca ccagatagca catacgctgc tggcgcgtct
    10501 aggtctgcgc tatgttcagc tgtcggtgat tatcatcggc cttattttcg gtctggcgat
    10561 gttctatgaa gtggccttta tcatgttagc gccgctggtt attgttattg ccgccgaagc
    10621 taaaattccg ttcctgaaac tggcgatccc ggcagtagca gctgccacta ccgcacattc
    10681 actgttccca ccgcagccgg gtccggtggc gctggtgaat gcttatggcg cggatatggg
    10741 gatggtttat atctatggcg tactggtgac gatcccaagt gtaatctgcg caggtctgat
    10801 cctgccgaag ttcctcggca atcttgagcg cccaacgcca tcattcctga aagcagatca
    10861 accggtagat atgaataatc tgccctcttt cggcgtttcg attctggtgc cgctgatccc
    10921 ggcgatcatt atgatctcta ccaccatcgc caatatctgg ctggtaaaag atacccctgc
    10981 ctgggaagtg gttaacttta ttggttcctc gccgattgcg atgtttattg cgatggtggt
    11041 tgcattcgta ctctttggca ccgcgcgtgg tcatgacatg cagtgggtga tgaacgcttt
    11101 tgaaagcgcg gtgaagagta ttgcaatggt gattctgatc atcggtgcgg gtggcgtgct
    11161 gaagcagacc atcatcgaca ccggcattgg cgacaccatc ggcatgttga tgtcccacgg
    11221 caatatctcg ccctacatta tggcatggct gatcactgtg ctaattcgtc tggcgacggg
    11281 tcagggtgtc gtttcggcga tgaccgccgc cgggattatc agtgctgcaa tccttgatcc
    11341 agcaaccggt cagctggttg gcgtgaatcc ggcgctgctg gtactggcga cggctgcggg
    11401 ttccaacacc ctcacccaca ttaatgatgc atctttctgg ttgttcaaag gttactttga
    11461 cctgtcagta aaagacacgt tgaaaacctg gggtctgctg gagctggtca actccgtggt
    11521 tgggctgatt attgtgctga ttattagcat ggtagcgtaa aagaacacca gagcctgcca
    11581 atggcaggct cagactgatg actaaagtct aaaaatgtgc ccggacagtc ccctcgcccc
    11641 tccggggaga gggttagggt gaggggaaca gaccagtaaa gtgcgctttg tttatgccag
    11701 atgcgacgct gacgcgtctt atctggccta caaagggcta acgtgcaggt tttgtaggtc
    11761 tgatagcgta gcgcctcagg taccagcatt agcaatgcgc tgtaattttt ttattgataa
    11821 acaaaagtca cgccaataat cgattgcaca ttccctgcag tcacctgccc tccggtacgt
    11881 gcgtaatttg ccgttaatcc cagacttacc gccgaagttc ctactgctcc taacgatacc
    11941 gtgttattcg ttggaataat cgtaccgttg cgcgtcaact gtacgccgac gccctgcgct
    12001 ggtgaaaacg acgcggtatt ggtgaaaatc gagttgcccg catctgcggt tgtgccggag
    12061 aggtaatacc ccaggttttg gcttttcgca caataaacgg taagaggaat tggcactgaa
    12121 ccagggtagt ccggcagagt aacggtgaca tcacgagcag aaacatcaca accgccagtg
    12181 ggcaccacca catcattatt ggcgtaaata ttccacacaa actgaaaatc atcgctgtta
    12241 tagttgttgg tctgtcgcaa aataagcacg gcaattaatg aaccagcttt aatcgccacc
    12301 ccgcccgcac tgctcacagg cgtcaaataa agcgccaccg gccacggctt atccgttctc
    12361 gaattataaa caacccgcgg cgtttcgctg gtggtcggaa atggatagct actgccacta
    12421 tattttacgg tcccggaaaa attagataac acgccgccgt aagccgagcc tcgttgcagt
    12481 gtgacatagt ctgtaatggt ttccggataa tcgttatggc aaaagatttg cgtcgaaaga
    12541 tctacgacca ggttttgccc cacattcacg acaggcgcaa ggtttacata aacattagcg
    12601 ctgccaccgc caatagggat agcggtacca ttggcggttt tacaggcgaa tgaccaggca
    12661 tttaccgacc agcccatcag cagtacagca aacagggtaa tagctcgttt cattacaatt
    12721 atctcttcgg gttcagctgt aggtataggt gatgctaatc actgcctgaa tggttccctg
    12781 agtggctccg ccatttaccg tcaatgctct gacctgtaac gggaagtgcg ctgattgtga
    12841 ggaatcatcc acctgaactg ttttggttgc gccagtattc aatgtgttgc cactgtcatc
    12901 ctgtagctct aactggatgt tttgcgcggt cccctggttt ttataatatc cggtgctgtc
    12961 ggctgccccg ctgaagctgg cagtgaccct cgacgttccc accggacaat tagtcaactc
    13021 aagcgcaaca tcatgccagg ccgatgccgc cccggcagac ataagactga aagaataaag
    13081 atcgccgaga tctaccgtgg cattggtggt ggaaaccgta cacggtttgg cgacgacctt
    13141 accgttcacc gtgatggtga catcggctgc ctgtatcgtc gcacttgcga gcgccaacat
    13201 tgccgccaat acatacccac gtttgcacca tttcatgagc atctccagtt actgatattc
    13261 aagagtgaag gtagccgtag cattgatatg ccccgcagtg acaggcacct gtgtcgccat
    13321 tagccgggcg taaaaattca gcgtatttgg tttacccggc gtcagggtcg tccacgaaat
    13381 cgcagacgat ggagcattaa ggggtatttg attttgctgc tcattcagaa gctgtattcc
    13441 cagtcccgca gccgctgaca ccgtattttc aagtgcaagc aggttggcat tgtggctatc
    13501 tgcaacgccg gtaaacccaa cttttacggc agaaacaggg tgacagcaaa atacgaaatg
    13561 gaacgacagg agtcgtcgcg ccaatgttgt taaattgctt cgccgcgttt tccatcagat
    13621 caacagtaaa attggttgat tcagcggcca cactacagcc gttatctctg acatagccgc
    13681 ggatagtaat cgtgctatcc gcagccaaaa cgcgacttac cgccagccac aaaaaagcgc
    13741 acagaagata aaaaggtttg tttctcatca cgccccctta acgacattca gctgatagct
    13801 gggttaataa ctgctgctga ctctctggtg gcagttgata attggcgaca cagtgagcat
    13861 tttcctcttc tccccatttc acctgaactt ttcccgctaa aggcattccg ctgaggtaaa
    13921 actgaccatt atccgcaaca atgccgctac tctggctact ctctgatgtc accatcgccc
    13981 caaacggcag cggcttatta ttgtgagtta gcgtcatgag cagttttatc ccaacgcgcg
    14041 ctttaaactc tgctcgcacg atcgccccac gagtgggaac aacgttagcg accgcgttat
    14101 ctaaatcgac gttatcagcc aaggtattgg tatccagcgc cactctattt tcccgatatt
    14161 cagtggcata aggcagcacg gcataaccac gccagtcggt acgcaccccc gtctggtttt
    14221 cgacttttgc atcttttgcg ccaggcgctt taacaagcac caccgtatcg tttaacggct
    14281 gccccagcgt tacgccattg gcatgagcca ataccccacc gctgactccg taatagagct
    14341 gcttaatatc atcgctatgg ctgtaaccga tattggcatt gccgtaacca ccgcgataat
    14401 tcagcgtggc gtagcctgta cttccgctat taccatcgcc tcccccggca tagccggttt
    14461 gcacgctata actgaggttg ttgtcttcca gcaacgtacc gtatacacca gccagattgg
    14521 tcatccgacc gttgagatcg tgtgacatgc tgtagctggc actggcatgt cgccactgag
    14581 atttactgtc agaacgcagc cagtggctga aaggaatatt gacgttaagc gctaacatct
    14641 gatcccgtcc tttttgccag gcgtttttcg tcaggctata gctgagcgtc cagttgatat
    14701 cttcgaacgc agtatttaat ccagcctgga attgctcatc gacattactc gttccccaat
    14761 aagtttgatg gctaccactc aaatacagtg ttgatgtgcg cccgagttgc tgagtaacgg
    14821 tgagctgtaa tttcccgcgt ttgttataag cgaggttgta atagtcggtg aatttcggct
    14881 taacctgaat aactccatcc tgtgtttcga tgttgtagcc attcattcga ctgtatgttg
    14941 tatcagcgaa attaaaatat ccgctggtcg aataacggta acccactaac tgaatattcg
    15001 tgcctgattc attgagcgat ttgttataga gaaaacgcac cgattgtccg tcatgctgac
    15061 tgtcatcggg aagtgtggaa ttagcctgcg tcatatccac agacagagcg cccagtgcct
    15121 ccatgttttt cccgataccg aaattaaaag cacgataacg atccgccagt tgcgttccac
    15181 catatattgt ccagccagcc ggaaggccgt ggagtaatgt actctggaaa aagcggggtt
    15241 tttcctgctg cgcatttcca ctacggtatt ctcctgccat aatggaataa cgagtatgcc
    15301 cttcacgttg caaaagcggg actgacgaat agggtacggt aaaaatctgc gtgctgccgt
    15361 cagcctcttt gatcgttacc tgcaagtcac cactattacc tgcggcatag atatcgttga
    15421 tggtaaaagg ccccggtggc accgtactat tataaatgcc atatccattt tgtttaatag
    15481 tgacctgtgc agtaccatga gcaataccgt ggatcaccgg ggcaaatcct ctttggctat
    15541 cgggtaacat attgtcatct gaggccaatt gtgcgccgcg aaagttaata ccatcgaaaa
    15601 tatcgccctg ggtataacca tcacccaacg tcagccggga acgtaaagga attatgtctc
    15661 gctcaatcca ggtaatgata tgctgccatt tatttttgct acctgatgaa ctgtcgctac
    15721 tgttataact ccaggtggta ttgtcgcgta aacgccacgc accaatattt aacccactct
    15781 gtaggtttaa atatgcataa tggctgttac ccccaatccg attctgtaca ctatttccgc
    15841 tgaaattata attgagcaat ccggcattaa taccgggatc ccataactca ggaggaatat
    15901 aaccacgcgc gcgattactc ataaatgcct gagggatcgt caggttcagt cgctgctgac
    15961 caacatccag atgcgcagta gcgtcctgga ccattgtggt taatggcaca caggcatcat
    16021 ccgccagcag attcataccg gcgacagaag ccgtattcag ccccatactg gcgagttgcg
    16081 cgcgtgtcag gcagggaaca atcccttgtt cactgtcgcc cgtattaaat gtgacatcac
    16141 gcgttgccat ataaccatta ttcaaataga tatcgacgcg atacgtccct ggcggtaatt
    16201 cttgcccatt ttcaaaacgc gataaatcgg ccacagcctg gggatcatcc gctaaaaagc
    16261 gcagattaaa atagaggtcg gcagatgaca aaggtgcctg tgcggcaaaa gcacaggcga
    16321 caacgagtcg gacaaaaaaa ccagccaaac gatgcttacg aatatgcaag cattgtgtgt
    16381 ttcgctgg
//
LOCUS       DAJTNM010000082        16198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTNM010000082 DAJTNM010000000
VERSION     DAJTNM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.082"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..162
                     /locus_tag="OO172_002761"
     CDS             <1..162
                     /locus_tag="OO172_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5512888.1"
                     /translation="SKGWPEIFAEAAHTLAFETLEHATRADHIDSDRYLIKSTYYNLF
                     SGEYSNKKT"
     gene            complement(442..1604)
                     /locus_tag="OO172_002762"
     CDS             complement(join(442..1343,1343..1604))
                     /locus_tag="OO172_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HCR5512889.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            complement(2007..2252)
                     /locus_tag="OO172_002763"
     CDS             complement(2007..2252)
                     /locus_tag="OO172_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512890.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(2897..2972)
                     /locus_tag="OO172_002764"
     tRNA            complement(2897..2972)
                     /locus_tag="OO172_002764"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2937..2939),aa:Phe,seq:gaa)
     gene            complement(3078..3785)
                     /locus_tag="OO172_002765"
     CDS             complement(3078..3785)
                     /locus_tag="OO172_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5512891.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            4183..6318
                     /gene="speC"
                     /locus_tag="OO172_002766"
     CDS             4183..6318
                     /gene="speC"
                     /locus_tag="OO172_002766"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5512892.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LVIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVVMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(6367..7623)
                     /gene="nupG"
                     /locus_tag="OO172_002767"
     CDS             complement(6367..7623)
                     /gene="nupG"
                     /locus_tag="OO172_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5512893.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7825..8904)
                     /gene="mltC"
                     /locus_tag="OO172_002768"
     CDS             complement(7825..8904)
                     /gene="mltC"
                     /locus_tag="OO172_002768"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5512894.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(8969..9244)
                     /locus_tag="OO172_002769"
     CDS             complement(8969..9244)
                     /locus_tag="OO172_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5512895.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(9272..10324)
                     /gene="mutY"
                     /locus_tag="OO172_002770"
     CDS             complement(9272..10324)
                     /gene="mutY"
                     /locus_tag="OO172_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5512896.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQMLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPKFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            10485..11204
                     /gene="trmB"
                     /locus_tag="OO172_002771"
     CDS             10485..11204
                     /gene="trmB"
                     /locus_tag="OO172_002771"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5512897.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            11204..11530
                     /locus_tag="OO172_002772"
     CDS             11204..11530
                     /locus_tag="OO172_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5512898.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            11714..12433
                     /locus_tag="OO172_002773"
     CDS             11714..12433
                     /locus_tag="OO172_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5512899.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            12609..13655
                     /gene="ansB"
                     /locus_tag="OO172_002774"
     CDS             12609..13655
                     /gene="ansB"
                     /locus_tag="OO172_002774"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5512900.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            13772..14779
                     /locus_tag="OO172_002775"
     CDS             13772..14779
                     /locus_tag="OO172_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5512901.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(14835..16136)
                     /locus_tag="OO172_002776"
                     /pseudo
     CDS             complement(14835..16136)
                     /locus_tag="OO172_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(16136..>16198)
                     /locus_tag="OO172_002777"
     CDS             complement(16136..>16198)
                     /locus_tag="OO172_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5512902.1"
                     /translation="ALCFINQSIQQLKQPAQEAS"
BASE COUNT         4199 a         3908 c         4152 g         3939 t
ORIGIN      
        1 agtaaaggtt ggcctgagat atttgctgaa gccgcacata ccctggcatt tgaaacatta
       61 gagcatgcaa ctcgtgctga ccatatagat tctgatagat atctaataaa atcaacttat
      121 tataatttat ttagtggcga atattctaat aaaaaaacat agctgcagag caagcaattc
      181 ggttgttggc aagtcaaggg caaagcattc gcgtgagaat gggcgtatgc tttgtgccaa
      241 aagcaaacgt tgctaattct gcactgttca gcgatggcgc agtagagaca ggcactgcca
      301 ctgtccagtc ggcaaaagcg aacactttgc cactgcgggg ataaactgat tgaagctctc
      361 gcgcaaaatc acttattctt ttgcgttaat aaaatgtagg agatgggtca ttgatcctac
      421 ccacgtaata tggacacagg cctaagcgag gttcttgttt tcaaattgtt ccggactgag
      481 gccgccacac caactgtgcc gccgccaccg attgtaatca cattcgatat aattaaacac
      541 cgttgcccgc attatttccc ggctgataaa gtgttctcca tggatacatt ccactttcag
      601 cgaatgaaag aagctttcca cgcaggcatt atcgtagcag caaccttttg cgctcatact
      661 tccacgcaga ttatgccgtt tcagttgcgc ctgataatct gctgaacagt actggcctcc
      721 acggtccgtg tgaacgataa cgttccgggg cctcttacgc cgccacagcg ccatctgcag
      781 ggcatcgcag gccagttgcg ccgtcatgcg tggcgacatt gaccagccaa taacggcacg
      841 tgaccacagg tcaatgacca ctgccagata cagccagcct tcatctgtac gtaagtacgt
      901 gatgtctcct ggccacttct ggttcgggcc actggcgtaa aaatcctgct ccaacagatt
      961 ttctgacaca ggcaggccgt gtgcgcggta gctgaccggg ctgaacttcc gggaggcctt
     1021 tgccctcagt ccctgacggc gcaggcttgc cgccacggtt tttacgttaa aggggtaacc
     1081 ctgagcacgc agttcatccg tcaggcgtgg ggcaccgtaa cgctgttttg accgggtaaa
     1141 agccgcgagg acaacgctgt cgcagtgttg gcggaactgc tgacgcgtgc ttatccttgt
     1201 ccgccgctga caccacgtat accagccgct gcgggccacc cggagcacgc ggcacattgc
     1261 tttgatgctg aactcagcct gatgtttttc aataaagaca tacttcattt caggcgcttc
     1321 gcgaagtatg tcgcggcctt ttggaggata gccagctctt catcccgttc tgccagctgg
     1381 cgtttgagac gtgcaatctc ggtagacatc tccagttcac gttcagaaga cgtctgctga
     1441 ttttgctgtt tactgcgcca gttgtagagc tgtgattcat acaggctgag ttcacgggct
     1501 gcggcagtaa caccgatgcg ttcagcaagc ttcagggctt cactgcgaaa ttcaggcgaa
     1561 tgctgtttac ggggtttttt actggttgat actgtttttg tcatgtgagt cacctctgac
     1621 tgagagttta ctcacttagc cgcgtgtcca ctattgctgg gtaagatcag gctgaggagt
     1681 ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta acaaattcaa
     1741 tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta atgctctaac
     1801 ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct tttaatctca
     1861 ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt ttgatgttga
     1921 tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac tgttgcgata
     1981 taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac cagaccctga
     2041 ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt gtgtctgcct
     2101 ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag ctgcacgttg
     2161 agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca gtgcatccgc
     2221 tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt gactgtcgtg
     2281 ttgccggttg cgtccatttt gcggttaaac ccttttaatt caatttgtta aattgctttc
     2341 caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata tgcaatgggc
     2401 tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa cgttctatct
     2461 ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc ctatggatca
     2521 tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat cagagtgaaa
     2581 aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg tatgcgtttt
     2641 acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt agccaggtgc
     2701 tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc acagtgacca
     2761 gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc agtgacatca
     2821 gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca gcccgtaggc
     2881 tggttcttct aagtagtggt gcccggactc ggaatcgaac caaggacacg gggattttca
     2941 atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc tgattcgcct
     3001 cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc atcactttgc
     3061 cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag cggcattgcc
     3121 agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca gatgcgtaat
     3181 ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat cgacggtgtg
     3241 gtcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat cagtaatggg
     3301 atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc cgtaaagaaa
     3361 tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc gttcatcgcc
     3421 ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata attctgaata
     3481 aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc tttggcgact
     3541 gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc aagtagcgta
     3601 gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc aatccccagc
     3661 gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa gcgttggctg
     3721 agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa agggccgatg
     3781 accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact ttttgccttg
     3841 ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag gtgccatgat
     3901 tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct ccttacattc
     3961 tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc tcatgcggtg
     4021 aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg cttttactga
     4081 tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca gggttttcca
     4141 ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc aatgaatatt
     4201 gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt ggcgttggga
     4261 gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag tcgcagtggc
     4321 attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta ttccgaacat
     4381 gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca gcagtggctg
     4441 gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt ttatgacacg
     4501 ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca tcaacatggt
     4561 gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg tgagaacgtc
     4621 tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat tcatgaagga
     4681 tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa aacctatttt
     4741 gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt aacgcgtggc
     4801 gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc gctgattcag
     4861 gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt cattggcggt
     4921 attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga cgttgcgcca
     4981 gaaaaagccg acctgccgcg cccgtttcgc ctggtgatta ttcagctggg aacctatgac
     5041 ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctctg tgattacatt
     5101 ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc ggatagctcg
     5161 ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca gttggtgcac
     5221 aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa ccatatccgc
     5281 ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct ccatgcttct
     5341 accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat tcatgaaggg
     5401 gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc gcgcaaggct
     5461 attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga tggcaaattg
     5521 tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag ttttgagccg
     5581 ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt tgatccgtgc
     5641 aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag cgactttggc
     5701 gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc ggagaagtgc
     5761 gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa gctggcacaa
     5821 ctggtggtga tgctggcgca atttgaacag catattgagg atgactcgtc gctggctgag
     5881 gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac cctgcgccag
     5941 ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct acaaaaagcg
     6001 atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc gcatagcgct
     6061 tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg aattgcggca
     6121 gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga agtctggggt
     6181 ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt gccgggattt
     6241 tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa acgattgtac
     6301 ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc gtttttcttt
     6361 gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt tatatttgaa
     6421 catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca accatacggt
     6481 ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc tcacgatgcc
     6541 gccgaggata cagccgaagc cgttagtcat catcaggaac gtcccttgtg cactggcgcg
     6601 aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga agaagtcgaa
     6661 tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg gagtcgggtc
     6721 gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca tcattacgtt
     6781 cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg tttcagagat
     6841 ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg caaacatcgg
     6901 atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct gcagttccgc
     6961 gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa acagcgcgaa
     7021 tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag caaccggaat
     7081 atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc caatatacag
     7141 ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca tgataaagcc
     7201 gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc cggcattttg
     7261 caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag caaacgagtt
     7321 aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca tgaacagcgt
     7381 gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc atttgtcggc
     7441 cacaatcccc agtagcgcag gcataaatac cgcggcgata cccagtgagc tataaactgc
     7501 gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga gggtcgtcag
     7561 ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca gcttaagatt
     7621 catgttaatt tcctcacatc gtgattcgga tgagtgtttg aaaaagcaca tttgaccgtg
     7681 cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt ggcaaataat
     7741 tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt tgcccctgag
     7801 catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg cggtattcac
     7861 tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg tctgataaac
     7921 atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat tcgaaaagac
     7981 tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac gccgcgatgt
     8041 tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg cggtgccggt
     8101 atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg atttcccctg
     8161 cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc ccagcgcatc
     8221 ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg ccagaatcag
     8281 cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat atttgtgcgc
     8341 acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga tacgtagccc
     8401 gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc ttgcgcgacc
     8461 ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga aaggttcttt
     8521 cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat cgtcacccat
     8581 cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc cggcgatggt
     8641 ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat attgatcggt
     8701 gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc agatgttctc
     8761 aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt tgacccaggc
     8821 ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg gcgcaatcaa
     8881 agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt tttatgcaag
     8941 ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta tagccctcga
     9001 tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc agcttgcggt
     9061 gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt ttgtgctgcc
     9121 actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc tcgccggggt
     9181 acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa atcgttctgc
     9241 tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg cgtaactgct
     9301 gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct aagttatacc
     9361 agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc cacataggca
     9421 caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc gtcaggttat
     9481 cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg gcaaacttcg
     9541 ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc aatacttcat
     9601 cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcatctgt ttcggttttt
     9661 tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt tgtagcggac
     9721 agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc atcatcgcct
     9781 gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc catagtttat
     9841 tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc agtacgcgtt
     9901 tgacgttacc gtcgagaatc ggaaagtgct tgcccagaga aagcgagaga atcgcgcctg
     9961 cggtggaacg cccgacgccc ggtaacggcg cgacttcttc aaaggtttcc gggaatttac
    10021 caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg cgggcgtaat
    10081 agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg agatcggtca
    10141 ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca acctgagttt
    10201 gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt tgccagggca
    10261 gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat tgcgacgctt
    10321 gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga cggcagggtg
    10381 taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa ctaactttgg
    10441 ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa aacgacgtca
    10501 tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt tttgtgcgcc
    10561 gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaattactgg ccggtgatgg
    10621 gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt gaagcgccgg
    10681 tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct aaagatcgcc
    10741 ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg tgcctggctt
    10801 ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg gttgaagtgc
    10861 tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc cctgacccgt
    10921 ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc gaactggtaa
    10981 aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa ccttatgcgg
    11041 aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca gagagcaatg
    11101 attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt ggtcatcgtc
    11161 ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa agaaccgtag
    11221 ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat tttcggtggc
    11281 atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg atgactttat
    11341 taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc tggcctggga
    11401 aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg cgattgtgcg
    11461 taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt tcgacgtttg
    11521 gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg cgctacgctt
    11581 atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg ccgcatccgg
    11641 catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc tgatttgttc
    11701 ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag tgacggcaat
    11761 gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga ttgtcagccc
    11821 gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag acggcaacgt
    11881 gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg cgaaggatta
    11941 tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa gccgcgtcga
    12001 gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca gcagcaaaat
    12061 gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc gcattatcga
    12121 aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc gcgccgaagg
    12181 ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta atgaaatggg
    12241 cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg gaagcttggg
    12301 cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt tccagcagtt
    12361 tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag ccctggtcgg
    12421 gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc gtttaattct
    12481 tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg ttgtaaattg
    12541 tttaacgtca aatttcccat acaaagctaa gggataatgc gtagcgttca cgtaactgga
    12601 ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg ggttttagtg
    12661 gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc attgccggtg
    12721 gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta gaaaatctgg
    12781 ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag gtagtgaata
    12841 tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa attaacgccg
    12901 actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg gaagaaactg
    12961 cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc ggcgcaatgc
    13021 gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg gtagtgaccg
    13081 cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac accgtactgg
    13141 acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag tctgttaact
    13201 acggtcctct gggatacatt cacaacggta agattgacta ccaacgtacc ccggcacgta
    13261 agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg aaagtcggca
    13321 tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta gatgcgggct
    13381 atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc gtgttcgaca
    13441 ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc gtaccgacgg
    13501 gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc gcctctggca
    13561 cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa accaaagatc
    13621 cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc atcgtgccgg
    13681 ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat gataatcacc
    13741 ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg ttggcacggc
    13801 gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa ctgaaaatat
    13861 cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg ccatcagctt
    13921 aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg atgtctcttc
    13981 cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc tcgaacagac
    14041 ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag gaacgcccgc
    14101 gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc gtgggcgggt
    14161 ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag atttcaatta
    14221 tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc ctgcgctgga
    14281 aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag cggatgcata
    14341 ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta aaaaaattca
    14401 ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg ttaaatatga
    14461 caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag agtgttatga
    14521 agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac agacttttct
    14581 gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg aaaaagggca
    14641 atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga ttttgatgaa
    14701 aattgacgat atcaaccaag ctaatgaacg ctggaaaaaa gataccgaac aactgcggcg
    14761 caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata aaaagttatc
    14821 cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga tatttgcgga
    14881 atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatggcag caatggtttg
    14941 attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc actacccacc
    15001 ggcagtgtta acagcccaat acaaaggttg gccaccatca taataccgaa atgtactggg
    15061 tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag tacagccggt
    15121 gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag caatatgatc
    15181 agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat atcagcatta
    15241 gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat cgaagtggta
    15301 acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg gtaaatcagc
    15361 accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc ggtaaaaatg
    15421 ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa ggcatccaga
    15481 aacgctttac aggcctgagc aaaggttgct ctttctgaaa gggggtagcc acggcgttta
    15541 gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg caaatatccc
    15601 gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac aattaacacg
    15661 tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc ggtagaaaat
    15721 gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac ggcagcagct
    15781 gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac gttcacatga
    15841 ccaagcgaac caggaacgcg gccaaccatt acctaggcga ggttaatcaa acgtatcgca
    15901 ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc caacagggca
    15961 aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat atcccaggga
    16021 aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat gggcacacca
    16081 ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact atccattaac
    16141 tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa cacaaggc
//
LOCUS       DAJTNM010000083        16097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTNM010000083 DAJTNM010000000
VERSION     DAJTNM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.083"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(134..466)
                     /gene="emrE"
                     /locus_tag="OO172_002778"
     CDS             complement(134..466)
                     /gene="emrE"
                     /locus_tag="OO172_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5512903.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(811..1125)
                     /gene="fliE"
                     /locus_tag="OO172_002779"
     CDS             complement(811..1125)
                     /gene="fliE"
                     /locus_tag="OO172_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5512904.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1339..2997
                     /gene="fliF"
                     /locus_tag="OO172_002780"
                     /pseudo
     CDS             1339..2997
                     /gene="fliF"
                     /locus_tag="OO172_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            2990..3985
                     /gene="fliG"
                     /locus_tag="OO172_002781"
     CDS             2990..3985
                     /gene="fliG"
                     /locus_tag="OO172_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5512905.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3978..4664
                     /gene="fliH"
                     /locus_tag="OO172_002782"
     CDS             3978..4664
                     /gene="fliH"
                     /locus_tag="OO172_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5512906.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            4664..6037
                     /gene="fliI"
                     /locus_tag="OO172_002783"
     CDS             4664..6037
                     /gene="fliI"
                     /locus_tag="OO172_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5512907.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRSQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6056..6499
                     /gene="fliJ"
                     /locus_tag="OO172_002784"
     CDS             6056..6499
                     /gene="fliJ"
                     /locus_tag="OO172_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR5512908.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6496..7623
                     /gene="fliK"
                     /locus_tag="OO172_002785"
     CDS             6496..7623
                     /gene="fliK"
                     /locus_tag="OO172_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5512909.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSASLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGDDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7728..8192
                     /gene="fliL"
                     /locus_tag="OO172_002786"
     CDS             7728..8192
                     /gene="fliL"
                     /locus_tag="OO172_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5512910.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8197..9201
                     /gene="fliM"
                     /locus_tag="OO172_002787"
     CDS             8197..9201
                     /gene="fliM"
                     /locus_tag="OO172_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5512911.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDILPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9198..9611
                     /gene="fliN"
                     /locus_tag="OO172_002788"
     CDS             9198..9611
                     /gene="fliN"
                     /locus_tag="OO172_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5512912.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9614..9979
                     /gene="fliO"
                     /locus_tag="OO172_002789"
     CDS             9614..9979
                     /gene="fliO"
                     /locus_tag="OO172_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5512913.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            9979..10716
                     /gene="fliP"
                     /locus_tag="OO172_002790"
     CDS             9979..10716
                     /gene="fliP"
                     /locus_tag="OO172_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5512914.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10726..10995
                     /gene="fliQ"
                     /locus_tag="OO172_002791"
     CDS             10726..10995
                     /gene="fliQ"
                     /locus_tag="OO172_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5512915.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11004..11708
                     /locus_tag="OO172_002792"
     CDS             11004..11708
                     /locus_tag="OO172_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCR5512916.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIFLNGLMLA
                     LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFS
                     EIFNLLADIISELPLI"
     gene            11993..12616
                     /gene="rcsA"
                     /locus_tag="OO172_002793"
     CDS             11993..12616
                     /gene="rcsA"
                     /locus_tag="OO172_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5512917.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12660..12848)
                     /gene="dsrB"
                     /locus_tag="OO172_002794"
     CDS             complement(12660..12848)
                     /gene="dsrB"
                     /locus_tag="OO172_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5512918.1"
                     /translation="MKVNDRVTVKTDSGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13011..13238
                     /gene="yodD"
                     /locus_tag="OO172_002795"
     CDS             13011..13238
                     /gene="yodD"
                     /locus_tag="OO172_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5512919.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13536..14351
                     /locus_tag="OO172_002796"
                     /pseudo
     CDS             13536..14351
                     /locus_tag="OO172_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(14348..16042)
                     /gene="yedQ"
                     /locus_tag="OO172_002797"
     CDS             complement(14348..16042)
                     /gene="yedQ"
                     /locus_tag="OO172_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR5512920.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDHA"
BASE COUNT         4005 a         4147 c         4231 g         3714 t
ORIGIN      
        1 ttgatgtgcc ttgatctcat tatgcataaa ggcacaatga tcgcgcaaat gcaatcataa
       61 ttataaaggt gccccgaaca ttagccataa tatgcacaaa tcagattcct gttatctcaa
      121 ggagaattat tttttaatgc ggtgcacttc gtgacaacaa gttaatgact aacacaccgg
      181 cacaaatcaa catcatgcct atgatagctg gcaggtccag tcgttgacca aaaaatcccc
      241 aggaaagcaa actaatcaga acgataccga ctcctgacca gatagcataa gcaatccctg
      301 taggtatata agccagcgtc tgagctaata accagaatga tgcacaatag caaataattg
      361 tgccaacaga gggccataac cgtgtaaacc cctctgaaaa cttcattaag gttgtaccaa
      421 tgacctctgc aagtattgcg ccaccaagat aaatataagg gttcatagca tattctttcc
      481 tgttcatact ggagagaatt gtactacagt ttgaacacaa ctcaccgttt tcatcctagt
      541 gtatgtgtat ccaaggggct ctggcgtggc aagagataaa ggcaggaaca ataatgatca
      601 gaaggaaccc caggctaacg aatgagttca ctcttcctgg gagcaaagcg aagacaaaac
      661 atgttatcgc gtttacggat cgtggatggc agaaaacaac caggatcagg tatccatttt
      721 aaaccagaaa ttgagtgagt ttgccccatc catgccccac gctgtgggat cggatataat
      781 taaacaagca tagtcaacag gttacacaac ctacacctgc atagacatca cttcctgata
      841 cgccgccacc agcttattac gcacctgaat ccccatttgc atagaaactg aggctttttg
      901 catatcggtc atcacatcgt ttagcgccac gccgggttca ccgagggtga atttttctgc
      961 ctgcgtgcgg gcgactgttt gtgtatcgct tatgcgatcg atcgcggcgt gcagctgccc
     1021 ggcaaaacta atggtcggtt gcggcagtga ttcctgcgca cgcgcactca tcgccgtagc
     1081 ctgtaactgg ctgataaccc cttcaatccc ctgtatcgct gacattttcg tctcccggat
     1141 aatttctggt aacaaagcct accagtaagt caataagaca aaggcgctaa atagcaacaa
     1201 aaaaacgggt ttattggcgg atagaaaaaa cgaaagcaca aataatggga gcgtcaattt
     1261 ttcgagtttg ctgacccgag agtgagtctt gttccacttt gccaataacg ccgtccataa
     1321 tcagccacga ggtgcgcgat gaatgcgact gcagcccaga caaaatctct tgagtggctt
     1381 aatcgcctgc gtgcgaatcc gaaaattcca ttgattgtta ccggttccgc ggcagtggcg
     1441 gtcatggtcg cactgatcct gtgggcgaaa gcccccgact accgcacatt attcagcaat
     1501 ctttccgatc aggatggtgg cgcaattgtc agccaactga cgcaaatgaa tattccttac
     1561 cgcttcagcg aagccagcgg cgctattgag gttccggcag ataaagttca cgaactgcgc
     1621 ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg tcggtttcga actgctcgat
     1681 caggaaaagt ttggtatcag ccagttcagc gaacaggtga attatcagcg ggcgctggaa
     1741 ggcgagcttt ctcgtaccat cgaaactatc ggctcggtaa aaggggcgcg cgtacatctg
     1801 gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc atcggtgacg
     1861 gtaaatctgt tacccagccg cgcactcgat gaagggcaaa ttagcgctat tgtacatctg
     1921 gtttccagcg cggttgctgg tctgccgcct ggaaacgtca cgctggtgga tcagggcggg
     1981 catctgttaa cccagtccaa taccagcggg cgcgatctta atgacgctca gttgaaatat
     2041 gccagcgatg tcgaaggccg tattcagcgg cgtattgaag cgatcctgtc gcctattgtt
     2101 ggtaacggta atattcacgc ctaggtcacg gcgcaactgg acttcgccag taaagaacaa
     2161 acggaagaac agtatcgccc taacggtgat gaatctcatg cggcgcttcg ttcacgccag
     2221 cttaatgaga gcgagcaaag cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg
     2281 aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc ctccgacaaa tcaaaataac
     2341 cgccagcagc aggcgagcac caccagcaat agtgggccgc gtagcacaca gcggaatgaa
     2401 accagtaact acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg
     2461 caacgtctgt cagtcgcggt agtggtgaat tacaaaacct tgccagacgg taaaccattg
     2521 cctctcagca acgaacagat gaagcaaatt gaagctctga cccgcgaggc gatgggcttt
     2581 tctgaaaaac gcggtgattc gctcaatgtt gttaactcgc cgttcaatag cagtgacgaa
     2641 agcggcggag cactgccgtt ttggcaacag caagtgttta tcgatcagtt gctggctgcc
     2701 ggtcgctggt tgctggtact gctggtggcg tggttgctgt ggcggaaagc ggtacgcccg
     2761 cagctaacac gtcgcgccga ggcggtgaag actgtgcagc aacaggcgca ggcccgcgag
     2821 gaagtggaag atgcggtgga agtccgtctg agcaaagacg aacaactcca acaacggcgc
     2881 gctaaccaac gactgggggc agaagtcatg agccagcgta tccgtgaaat gtctgataac
     2941 gatccgcgcg tggtggcgct ggtcattcgc cagtggataa ataacgatca tgagtaacct
     3001 gacaggcacc gataaaagcg tcatcctgct gatgaccatt ggcgaagacc gggcggcaga
     3061 ggtgttcaag cacctctccc agcgcgaagt gcaaaccctg agcgctgcaa tggcgaacgt
     3121 cacgcagatc tccaacaagc agctaaccga tgtgctggcg gagtttgagc aagaagctga
     3181 acagtttgct gcactgaata tcaacgccaa cgattatctg cgttcggtat tggtcaaagc
     3241 tctgggtgaa gaacgtgccg ccagcctgct ggaagatatt ctcgaaaccc gcgataccgc
     3301 cagcggtatt gaaacgctca actttatgga gccgcagagc gccgccgatc tgattcgcga
     3361 tgagcatccg caaattatcg ccaccattct ggtgcatctg aagcgcgccc aggccgccga
     3421 tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg atgttgcgta tcgccacctt
     3481 tggcggcgtg cagccagccg cgctggccga actgaccgaa gtactgaatg gcttgctcga
     3541 cggtcagaat ctcaagcgca gcaaaatggg cggcgtgaga acggcagccg aaattatcaa
     3601 cctgatgaaa acgcagcagg aagaagccgt tattaccgcc gtgcgtgaat tcgacggcga
     3661 gctggcgcag aaaatcatcg acgagatgtt cctgttcgag aatctggtgg atgtcgatga
     3721 tcgcagcatt cagcgtctgt tgcaggaagt ggattccgaa tcgctgctga tcgcgctgaa
     3781 aggggccgag cagccactgc gcgagaaatt cctgcgcaat atgtcgcagc gtgccgccga
     3841 tattctgcgc gacgatctcg ccaaccgtgg tccggtgcgt ctgtcgcagg tcgaaaacga
     3901 acagaaagcg attctgctga ttgtgcgccg tcttgccgaa actggcgaga tggtgattgg
     3961 cagcggcgag gatacctatg tctgataatc tgccgtggaa aacctggacg ccggacgatc
     4021 tcgcgccacc accggcagag tttgtgccaa tggtcgagtc ggaagaaacc attattgaag
     4081 aggccgaacc cagccttgag cagcaactgg cgcaactgca aatgcaggcc cacgagcagg
     4141 gttatcaggc ggggattgcc gaaggtcgcc agcaaggtca cgaacagggc tatcaggaag
     4201 gactggccca ggggctggaa caaggtctgg cagaggcgaa ggctcaacaa gcgccaattc
     4261 atgcccggat gcagcaactg gtcagcgaat ttcaaactac ccttgatgcg cttgatagtg
     4321 tgatagcttc gcgcctgatg cagatggcgc tggaggcagc acgtcaggtg atcggtcaga
     4381 caccaacggt ggataactcg gcactgatca aacagatcca acagttgttg cagcaagaac
     4441 cgttattcag cgggaaacca cagctgcgcg tacacccgga tgatctgcaa cgtgtggatg
     4501 atatgctcgg cgcaacttta agtttgcatg gctggcgctt gcggggcgat cccaccctcc
     4561 accctggcgg atgtaaagtc tccgccgatg aaggcgatct cgacgccagt gtcgccactc
     4621 gctggcaaga actctgccgt ctggcagcac taggagtggt gtaatgacca cgcgcctgac
     4681 tcgatggcta accacgctgg ataactttga agcaaaaatg gcgcagttgc ctgcggtacg
     4741 tcgctacggg cgattaaccc gcgctaccgg gctggtgctg gaagccaccg gattacaatt
     4801 gccgctcggc gcaacctgtg tcattgagcg ccagaacggc agcgaaacgc acgaagtaga
     4861 aagcgaagtc gttggcttta acggtcaacg gctgttttta atgccgctgg aggaagtgga
     4921 aggtgtcctg cccggcgcgc gtgtatatgc caaaaacatt tcggcagaag ggctgcaaag
     4981 cggcaagcag ttgccgctcg gtccggcgtt attaggtcgt gttctggacg gtagcggtaa
     5041 accgctcgat ggcctgccct tccccgatac gacggaaacc ggtgcgctga ttaccccgcc
     5101 atttaacccg ttgcaacgta caccgattga acatgtgctg gacaccggcg tgcgcccaat
     5161 caatgccctg cttaccgtcg gacgcgggca gcgtatgggg ctgtttgccg ggtcgggcgt
     5221 tggtaaaagt gtgctgctgg ggatgatggc acgttacacc cgcgccgatg tcattgtcgt
     5281 gggtttgatt ggtgaacgtg ggcgcgaagt aaaagatttt attgagaata tcctcggtgc
     5341 cgaaggacgt gcacgctcag tggtgattgc cgctccggcg gatgtttctc cgctcctgcg
     5401 aatgcaaggt gccgcctatg ccacgcgcat tgccgaagat tttcgcgatc gtagtcagca
     5461 tgtgttgctg attatggact ccctcacccg ctacgcgatg gcccagcgtg aaattgcgct
     5521 ggcgattggc gaaccacctg ccactaaagg ttatccaccg tcggtgtttg ccaaattacc
     5581 ggcactggtc gagcgtgccg gtaatggcat tagcggcggc ggctcgatta ccgcgtttta
     5641 taccgtgctc accgaaggcg atgaccagca ggaccccatt gctgactccg cgcgggcaat
     5701 cctcgacggt cacattgtgc tgtctcgccg actggcggaa gccgggcact atccggctat
     5761 cgatattgaa gcgtcgatca gtcgcgcaat gacggcgttg atcagtgagc aacattacgc
     5821 gcgagtgcgc accttcaaac agctgttgtc gagttttcag cgtaaccgcg atctggttag
     5881 cgtcggcgcg tatgccaaag gcagcgatcc gatgctcgat aaagccatcg ccctgtggcc
     5941 gcagctggag ggctatttgc aacaaggcat ttttgaacgc gcggactggg aagcgtctct
     6001 ccaggggctg gagcgtattt tcccgacagt gtcataaccc aggagataac ggcagatggc
     6061 ggaacatggt gcgctggcga ccctgaaaga tctggcagaa aaagaggtag aggatgccgc
     6121 tcgcctgctg ggtgaaatgc gtcgcggatg tcagcaggcg gaagaacagc tcaaaatgct
     6181 gatcgattat cagaatgaat atcgcaataa cctcaatagc gatatgagtg ccgggatgac
     6241 cagcaaccgc tggatcaact atcagcagtt tatccagacg ctggaaaaag ccattactca
     6301 gcatcgccag caacttaatc agtggacgca gaaagttgac attgccctga acagctggcg
     6361 agaaaagaaa caacgtttgc aggcctggca gacactgcag gaacggcaat ccacggcggc
     6421 actgcttgca gaaaaccgcc tcgatcagaa aaagatggat gagttcgccc agcgcgccgc
     6481 catgaggaaa cctgaatgat tcgcttagcg cccttgatta ccgccgacgt tgacaccacc
     6541 acattgcctg gcggcaaagc cagcgatgct gcacaagatt ttctaacgtt gttgagcgaa
     6601 gcattagcag gcgagacaac aactgacaaa gcggcccccc agttgctggt ggcaacagat
     6661 aagcccacga caaaaggcga gccgctggtc agcgatattc ttgccgatgc gcaacaagcg
     6721 gatttactga tccctgtgga tgaaacactt cctgtcatca acgacgaaca atccacatca
     6781 acaccgttaa ccaccgctca gacaatgacg ttggctgcgg tggctgacaa aaatacgaca
     6841 aaagacgaaa aagcggatga tcttaatgaa gacgtcaccg caagtctgag cgcccttttt
     6901 gcgatgttgc cgggttttga caatacgccc aaagtgactg atgcaccgtc aaccgtgtta
     6961 ccggcagaga aaccaacgct cttcacaaaa ctgacttctg cgcaactcac tacagcacag
     7021 cctgatgacg cccccggcac accagctcag ccattaacac cgctggtagc agaagcccag
     7081 agtaaagcgg aagtcatcag cacgccttcg ccggtgaccg ctgacgccag cccgctaatc
     7141 actccacacc agacacagcc actgcccacc gtcgccgcgc ctgttttgag tgcatcgctg
     7201 ggttctcacg aatggcaaca atcattgagc cagcatattt cgctgttcac ccgccagggg
     7261 caacaaagtg cagagttgcg tctgcaccca caggatttag gtgaagtgca aatttccctc
     7321 aaagtggatg ataaccaggc gcagatccag atgatttcac cgcatcagca cgtacgcgcc
     7381 gccctggaag cagcgctgcc ggtactgcgt acgcagctgg ccgaaagtgg cattcagtta
     7441 gggcaaagca acattagtgg cgaaagcttt agtggtcagc agcaggccgc ttcccagcaa
     7501 cagcaaagcc aacgcacagc aaaccatgaa cctctggcgg gggatgacga cgatacgctt
     7561 ccggttcccg tctctttaca agggcgtgta acaggtaaca gcggcgttga tattttcgcc
     7621 taacgtcaga ggtagcaccg taatccgcgt cttttccccg ctttgttgcg ctcaagacgc
     7681 aggataatta gccgataagc agtagcgaca caggaagacc gcaacacatg actgattacg
     7741 cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat tctggtattc attaccctcg
     7801 cggcctgtgc cagcgcaggt tacagctact ggcattcgca tcaggttgcc gctgacgaca
     7861 aagcgcagca acgcgtcgtg ccctccccgg tcttctatgc gctggatacc tttacggtca
     7921 atttgggcga tgcggatcgc gtgctttata tcggcataac cctgcgactg aaagatgaag
     7981 caacccgctc gcggctgagt gagtatttgc cggaagtccg tagtcgcttg ctgttactgt
     8041 tttcacgtca ggatgctgcc gtactggcga cagaagaagg caagaaaaac ctgattgccg
     8101 agattaaaac cacactttcc accccgcttg ttgccgggca accgaaacag gatgtcaccg
     8161 acgtgctgta taccgctttt attctgcgat aacgacatgg gcgatagtat tctttctcaa
     8221 gctgaaattg atgcgctgtt gaatggtgac agcgaagtca aagacgaacc gacagccagt
     8281 gttagcggcg aaagtgacat tcgtccgtac gatccgaata cccaacgacg ggttgtgcgc
     8341 gaacgtttgc aggcgctgga aatcattaat gagcgctttg cccgccattt tcgtatgggg
     8401 ctgttcaacc tgctgcgccg tagcccggac attaccgtcg gggccatccg cattcagcca
     8461 taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc tgaacctaat ccacctgaaa
     8521 ccgctacgcg gcacagggct ggtagtgttc tcgccgagtc tggtgtttat cgccgtggat
     8581 aacctgtttg gcggcgatgg acgcttcccg accaaagtgg aaggtcgcga gtttacccat
     8641 actgaacagc gcgtcatcaa ccgtatgttg aagctggcgc tggaaggcta tagcgacgcc
     8701 tggaaagcga ttaatccgct ggaagttgag tacgtgcgtt cggaaatgca ggtggaattt
     8761 accaatatca ccacctcgcc gaacgacatt gtggttaaca cgccgttcca tgtggagatt
     8821 ggcaacctga ccggcgaatt taatatctgc ctgccattca gcatgatcga gccgctacgg
     8881 gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg aagatcagaa ctggcgcgat
     8941 aacctggtgc gccaggtgca gcattcacag ctggaactgg tggccaactt tgccgatatc
     9001 tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg acatcctgcc gatagaaaaa
     9061 cccgatcgca tcatcgccca tgttgacggc gtcccggttc tgaccagtca gtacggcact
     9121 ctcaacggtc agtatgcgtt acggatagaa catttgatta acccgatttt aaattctctg
     9181 aacgaggaac agcccaaatg agtgacatga ataatccggc cgatgacaac aacggcgcaa
     9241 tggacgatct gtgggctgaa gcgttgagcg agcaaaaatc aaccagcgaa aaaagcgctg
     9301 ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag cggaacgttg caggatatcg
     9361 acctgattat ggatattccg gtcaagctga ccgtcgagct gggccgtacg cggatgacca
     9421 tcaaagagct gttgcgtctg acgcaagggt cagtcgtggc gctggacggt ctggcgggcg
     9481 aaccgctgga tattctgaac aacggttatt taatcgccca aggcgaagtg gtggttgttg
     9541 ccgataaata tggcgtgcgg atcaccgata tcattacccc gtccgagcgg atgcgccgcc
     9601 tgagccgtta gtgatgaata accatgctac tgtgcaatct tccgcgccgg tttctgctga
     9661 gccactgctg caggtgagcg gcgcactcat cgccattatt gccctgatcc tcgctgctgc
     9721 ctggctggta aaacggctgg gatttgcccc taaacgcact ggcgttaacg gtctgaaaat
     9781 tagcgccagt gcttcgctgg gcgcgcgtga acgggtcgtg gtggtcgatg tggaagatgc
     9841 acggctggtg ctcggcgtta ccgcagggca aatcaatctg ctgcataaac ttcccccttc
     9901 agcaccaacg gaagagatac cgcagaccga ttttcagtcg gtcatgaaaa atttgcttaa
     9961 gcgtggcggg agatcctgat gcgtcgtttg ttttctgtcg caccggtcct tctctggctg
    10021 attatgcccc tcgccttcgc gcaactgccg ggtatcacca gccagccgct acctggcggt
    10081 ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca tcacctcgtt gacgttcatt
    10141 ccagcgattt tactgatgat gaccagtttc acccgcatca ttattgtttt tggtttattg
    10201 cgtaacgcgc tgggaacgcc ctccgcgcca cctaaccagg tattgctggg gctggcactg
    10261 tttttgacct tttttattat gtcgccagtg atcgacaaaa tttatgtcga tgcgtaccag
    10321 ccattcagcg aagagaaaat ctcaatgcag gaggcgctgg aaaaaggggc gcagccgctg
    10381 cgtgagttta tgctgcgtca gacccgcgag gcagatttag ggttgtttgc cagactggcg
    10441 aataccggcc cgttgcaggg gcctgaagct gtaccgatgc gcattttgct cccggcctac
    10501 gtgaccagcg agttgaaaac cgcatttcag ataggcttca cgattttcat cccttttttg
    10561 attatcgacc tggtgattgc cagcgtgttg atggcattgg ggatgatgat ggttcccccc
    10621 gccaccattg ctctgccctt taaactgatg ctgtttgtac tggtggatgg ctggcaattg
    10681 ctggtcggtt cgctggcgca gagcttttac agctagagag gcaaaatgac acctgaatcg
    10741 gtcatgatga tgggaactga agcgatgaaa gtcgcgctgg cactggcagc cccgctgttg
    10801 ctggtagcgc tggtcacggg ccttatcatc agtattttgc aggcagccac gcagattaac
    10861 gaaatgacgc tgtcgtttat tccgaaaatc atcgccgtat ttatcgccat cattattgcc
    10921 ggaccgtgga tgctcaatct gttgctggat tacgtccgca ccttgttcac taacctgccg
    10981 tatatcatcg ggtagcccgt acaatgatgc aggagacaag cgatcaatgg ctttcctggt
    11041 taagcctgta cttctggccg ttactgcgcg tgctggcgct catttctacc gcgccaattc
    11101 tgagcgaacg cagcgtgccg aaacgggtaa aactgggtct ggcaatgatg atcacgttcg
    11161 ccattgcccc atcattacct gccaacgatg ttcctgtttt ttcgttcttt gctctgtggc
    11221 tggccgtgca gcagatcctg atcggcattg cgcttggttt taccatgcaa tttgcctttg
    11281 ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat ggggctgtca tttgcgacat
    11341 ttaacggtca tttatggttg atttcactgc tggtcgatac ctttcacacc ctgccgattg
    11401 gtagcgaacc gttgaacagc aatgcgtttc tggcacccac caaagcaggg agtttgattt
    11461 tccttaacgg gctgatgctg gcgttaccgc tcattactct gttgctgacc ctgaatctgg
    11521 cattaggttt actgaatcgt atggccccgc aattatccat ttttgttatt ggatttccat
    11581 taactctgac tgtcggcatc tctttaatgg cggcattaat gccgttaatt gcgccttttt
    11641 gcgaacattt attcagtgaa atttttaatt tgctggctga tattattagt gaattaccat
    11701 taatataatt ccgtaacgtt tatcatgcta ccctaaggat tatccgaaaa ataatatctg
    11761 cgaacatctt ccaggatact cctgtagcga aatatttgtt ttaagctcac tcacatatcg
    11821 caacatttac tttaagacaa ttccaggcaa attatacaac actttacggg atagtaagtc
    11881 cgcctgaaaa attgcgagag tggcgcatta ggtgacccat gttgttccgt ttagtcatga
    11941 tgaaatattc aggtaagggg aattatcgtt acgcattgag tgagggtatg ccatgtcaac
    12001 gattattatg gatttatgta gttacacccg actaggttta accgggtatc tgttgagtag
    12061 aggggttaaa aaaagagaaa tcaacgacat tgaaaccgtt gatgaccttg ccatagcttg
    12121 tgattcacag cgtccttcag tggtgtttat taatgaggac tgtttcatcc acgatgcttc
    12181 taacagtcag cgcatcaagc acatcattaa tcaacatccc aatacgttat ttatcgtttt
    12241 tatggcaatt gccaatgttc attttgatga atatctattg gtcagaaaaa atttattgat
    12301 cagttctaaa tcgattaaac cggaatctct cgacgatatc cttggcgata ttctgaaaaa
    12361 agagacaacg ataacctcgt ttttaaatat gccgacgtta tcattgagcc gaaccgaatc
    12421 gagtatgttg cgaatgtgga tggcaggtca gggaaccatt caaatctccg accaaatgaa
    12481 tatcaaagcc aagaccgttt catcgcataa aggtaatatt aaacgtaaga tcaaaacgca
    12541 taataaacag gttatctacc atgtcgtccg actgacggat aatgtgacta atggtatttt
    12601 tgtcaacatg cgctaacaca ttctgactgg tggtttccca ccagtcaggc tgaataagat
    12661 tactctgctt tctcaacaaa gataccgtcc tgatgccctg cttcattaaa gaaccagatg
    12721 ccgagcgggt agtcttccag cgcaaccagg tacattgtgc cttcactaaa ctcctcaact
    12781 gccagtacca ctccaggacg acgcggaccg ctatccgttt tgactgttac ccgatcattc
    12841 accttcatcg ttttcctcct gtggctttgt gccagtgtag aacaatttcg tttttctggc
    12901 agcgccaagc gcgcgcgagt gctgattttc tcgacggtct atacttaaga gatgccaggc
    12961 ggacttaacg actggcggca acaacagagt aacggttgcg aggaaagatg atgaaaaccg
    13021 caaaagtgta cagcgatacc gcaaaacgtg aggtcagcgt cgatgtcgat gccctgctgg
    13081 cggcgatcaa tgaaattagc gaaagcgaag ttcatcgcag ccagaacgat tctgaacacg
    13141 ttagcgtcga tggacgtgaa tatcatacat ggcgtgaatt ggcggatgcc ttcgaactgg
    13201 atattcatga cttcagcgtc tctgaagtga atcgttgaat gcacaataaa aaaatcccga
    13261 ccctgagggt gtcgggatga aacttgctta agcaagaagc acttaaaaaa ttcgttacac
    13321 caggaaatct gatgtgttca tcaccttatc tgcaattttt ttcgctgaca agaaaatatt
    13381 cgcgctatga atgattaacg ctacttatga atagaaatgt gacgcgcgtc accccttggt
    13441 ggctctggtg tcaacgggat cgctcccgaa aaaatcatct ttgattattt gtgctgtttt
    13501 gagaatcatc ctggtaaggg gagtactgcg cgaccatgtt ttcaattcaa caaccactac
    13561 tggtttttag cgatcttgat ggcaccctgc tggacagtca tagttatgac tggcaaccgg
    13621 cagccccctg gctcagccgt ttacacgaag ccaatattcc cgtcattctc tgtagcagta
    13681 aaacttcagc ggaaatgctg tacttgcaaa aaatgttggg gctacaaggc ttaccgctga
    13741 ttgcagagaa tggcgcagtg atccagcttg ctgaacaatg gcaggatata gacggttttc
    13801 cacgcatcat ctcaggtatt agccatggcg aaatctgcca ggttttaaat acgctacgcg
    13861 agaaagaaca ttttaaattc acgacttttg atgatgtcga cgatgcaacc attgccgaat
    13921 ggacgggatt aagccgtagc taggcggcgc tgacgcagct gcatgaggcg tcggtaacgc
    13981 taatctggcg cgacagtgac gagcgtatgg cacaatttat cgctcgtctg aacgaactgg
    14041 gcttgcagtt tatgcagggt gcgcgcttct ggcacgtact ggatgcctct gccggaaaag
    14101 atcaggctgc caactggatt atcgcgacct atcaacaatt gtcaggcaga cgcccaacca
    14161 cacttggcct gggcgatggg ccaaacgatg cgcctttact ggaggtaatg gattacgcgg
    14221 tgattgtgaa agggctaaac cgtgaagggg tacatctgca tgatgaggat ccggcccgcg
    14281 tctggcgaac gcagcgtgaa ggaccggaag gctggcgtga agggctggac cattttttct
    14341 ccgcccgtta agcgtgatcg ctcgcgaata cccgattgcg cccggcctgt tttgcgaggt
    14401 aaagccgacg gtcggccaat gactggagtt gttcaaaatc ataatcaccg gtttcctcgc
    14461 tgctacttac ccccagcgag gcactgatgc gtatcgttgt actcttggcg atcagcatct
    14521 ctttttcatt taacttcagg cgaatacgtt ctgcgacctc cgcagcctgc gtcagattcg
    14581 cgcctggcag aatcacacaa aactcctcac caccgacccg cccggcaacg tcctgcgcac
    14641 gcaaggaact gctaattaat ccggcagcat gagaaagaac acggtcgccc gcctgatgac
    14701 caaagcggtc attaatcgct ttaaaatggt caagatcgac ctgaatgaca gaaaaaggat
    14761 gttggtgcgt ctgacacaat ttagcgagcg gacgggcttt ttcgaacagt gcgccacggt
    14821 tatataaacg cgttaaggtg tcgtgccacg cctgccactg caacgagctt tgcagaacat
    14881 acatgttgct gaccatccgg cgaatcacat accaggaaag gagtaacatg gtggtaaaga
    14941 gcgcccacag cagggttaat gcaatgctga tactgccgaa atcgccgcgc acgccttcgc
    15001 ttagcgtatg gacgcgcgcc agcacaccgt cgaaatggtc cagacgttcc cagctaacat
    15061 agcgactgtc catacgaatg ccgccccgcg tgtcatgttc cattgcctgc gccagcaagg
    15121 ccagttcacg aggatcaaaa atattccctg ttggatggtc aggattggaa gaggtcaaaa
    15181 atatcagctt actgtcatag agctgatact caccatcgag gtttttatcg atggcgtttc
    15241 ttaaaaattg ctgcatagta cgcacgggaa tactcatccc cagcacgcca taccagtagt
    15301 tattactgtc taccggaaca ctgacggtaa cctgcggttc agtattgctg gcgtgttccg
    15361 gttgcgaagt gaaccagcgt accgcgcggt gacgattttc tcgttgcgaa tggccgataa
    15421 accagggttg ggtgacatag ccgtaataac gcgttggtac attgcgcata aacaaggtcg
    15481 gctgcgtcga aacgtaaaat ccagcacgcg agacatacat cgcctgttca accatcgacg
    15541 aggagttgtg cgccagtcgc agcaagtaac caacttccag tgcagcggta atttcattgt
    15601 cgaggctttc attttcgcga gacaggaaat tcccctcgct gactaatgca tccgatacgc
    15661 cattgactgg cagggtgcgc cgtcggttga gttcgatttg ccaggcgtgc tcgtcgcgat
    15721 gctgttcgaa ctcggttacg gcattacgca gagaggtgaa atccagtggc gccacgagag
    15781 cttcgcgcat gccattacgc aaaaagatca gtttgtcgac gttatactgc aaatgcttat
    15841 cgagcgcgtt ggctacgttt tccagatgat tacgctggct ggagatatag gcatcttcca
    15901 gcaccaccac ttcacgccag gtgagcaagg tggaaaaaag caataccacg ataaagcaga
    15961 gattaacgac atgaccgggc cccaggcggc gtgcgagttt tttcaaccag ctctggtttt
    16021 ccatttttgt ctcgtgctgc accctgacga ctcccaacct gcttttttat gattctggca
    16081 taacccggca atagcgt
//
LOCUS       DAJTNM010000084        16082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTNM010000084 DAJTNM010000000
VERSION     DAJTNM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.084"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..2071
                     /gene="pbpC"
                     /locus_tag="OO172_002798"
                     /pseudo
     CDS             1..2071
                     /gene="pbpC"
                     /locus_tag="OO172_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2221..2652
                     /gene="ndk"
                     /locus_tag="OO172_002799"
     CDS             2221..2652
                     /gene="ndk"
                     /locus_tag="OO172_002799"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5512921.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2802..3956
                     /gene="trmG/rlmN"
                     /locus_tag="OO172_002800"
     CDS             2802..3956
                     /gene="trmG/rlmN"
                     /locus_tag="OO172_002800"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5512922.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4241..5254
                     /gene="rodZ"
                     /locus_tag="OO172_002801"
     CDS             4241..5254
                     /gene="rodZ"
                     /locus_tag="OO172_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5512923.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5281..6399
                     /gene="ispG"
                     /locus_tag="OO172_002802"
                     /gene_synonym="gcpE"
     CDS             5281..6399
                     /gene="ispG"
                     /locus_tag="OO172_002802"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5512924.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6510..7784
                     /gene="hisS"
                     /locus_tag="OO172_002803"
     CDS             6510..7784
                     /gene="hisS"
                     /locus_tag="OO172_002803"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5512925.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7802..8422
                     /gene="yfgM"
                     /locus_tag="OO172_002804"
     CDS             7802..8422
                     /gene="yfgM"
                     /locus_tag="OO172_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5512926.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8433..9611
                     /gene="bamB"
                     /locus_tag="OO172_002805"
     CDS             8433..9611
                     /gene="bamB"
                     /locus_tag="OO172_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5512927.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9729..11201
                     /gene="der"
                     /locus_tag="OO172_002806"
     CDS             9729..11201
                     /gene="der"
                     /locus_tag="OO172_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5512928.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11271..11486
                     /locus_tag="OO172_002807"
     CDS             11271..11486
                     /locus_tag="OO172_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5512929.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11483..12853)
                     /gene="xseA"
                     /locus_tag="OO172_002808"
     CDS             complement(11483..12853)
                     /gene="xseA"
                     /locus_tag="OO172_002808"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5512930.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13015..14481
                     /gene="guaB"
                     /locus_tag="OO172_002809"
     CDS             13015..14481
                     /gene="guaB"
                     /locus_tag="OO172_002809"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5512931.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14550..>16082
                     /gene="guaA"
                     /locus_tag="OO172_002810"
     CDS             14550..>16082
                     /gene="guaA"
                     /locus_tag="OO172_002810"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5512932.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVV"
BASE COUNT         3743 a         4007 c         4543 g         3789 t
ORIGIN      
        1 tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc tcggtggcgc
       61 gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc acgctcacta
      121 tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa attctccagc
      181 tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg atcttgtatc
      241 ttaaccgcgc tccgtttggc gttacgttgc aggggatcgg tgcggcaagt tgggcttatc
      301 tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg gtgttaccgc
      361 aagtgcccag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc gcgcgtaata
      421 aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta aaagagtcaa
      481 gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg ctgttttcgc
      541 gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat gccggtcttc
      601 aacgacgtct ggaagaactg gcgcaaactg gaaagggcgg ttgccaccgc gcagctcact
      661 ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg gatcggttga
      721 tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc gatcgccagg
      781 atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga tccacccggc
      841 atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta actttgatag
      901 cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga acttacctgc
      961 tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac gcaatgttgg
     1021 attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc tcggcggcgc
     1081 tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc acggcaaggc
     1141 aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa tgtcgtcggg
     1201 ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc cggatagtgc
     1261 cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct atcgtgacgc
     1321 ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca gacctgacgg
     1381 cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga atcaggtcaa
     1441 taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga actcagtcac
     1501 tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg gtaactgtcg
     1561 ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt tactgtcaga
     1621 gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag ggcagcgcgt
     1681 cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc taccgctgga
     1741 accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct cgacaatttg
     1801 cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc gcgatggcgc
     1861 gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat caagtggagg
     1921 ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg ggcgcaacgt
     1981 gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg atgtggggca
     2041 aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg ttgctaaaac
     2101 tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg ttgctcatta
     2161 taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac agaggtaaaa
     2221 atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa aaacgtcatt
     2281 ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac caaaatgctg
     2341 cacctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa accgttcttt
     2401 gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct ggaaggtgaa
     2461 aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa cgcactggct
     2521 ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca cggttctgat
     2581 tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga agtgtgcccg
     2641 cgcacccgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg cgccagaatt
     2701 tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt ttttcaaccc
     2761 tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa caattagtca
     2821 cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc aaccgtcagc
     2881 agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat caggtgatga
     2941 agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc aacaaagtgt
     3001 tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt gaagaacagc
     3061 gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc gtcgaaacgg
     3121 tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg gggtgtgcgc
     3181 tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg cgggtatcgg
     3241 aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa gtcaccggtc
     3301 agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc aacctgaaca
     3361 acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg tctaaacgtc
     3421 gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc gatatgatcg
     3481 acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac gaaattgtgc
     3541 cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt tatctggaga
     3601 aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac cacgttaacg
     3661 acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg tgtaagatca
     3721 acctgatccc gtggaacccg ttcccgggcg cgccgtatgg acgcagttcg aacagccgta
     3781 tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg cgtaaaacgc
     3841 gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc gaccgtacga
     3901 aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg gtctgatagc
     3961 cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga agtattaagt
     4021 tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca gtatgtaacg
     4081 atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac agacggtctt
     4141 atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag catgatggtt
     4201 caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg aagccacgca
     4261 cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg aacaactagg
     4321 acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg tacgcgacat
     4381 tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat atatccgctc
     4441 ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg aaaagcaggc
     4501 tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta aacgccgcaa
     4561 aaaacgcgac ggctggctga tgaccttcac ctggctggtg ttgtttgtgg ttatcggcct
     4621 gagcggtgcc tggtggtggc aagaccacaa agctcagcag gaagagatca ccactatggc
     4681 cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg ttccgttaaa
     4741 tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg tggatactac
     4801 cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg atccgcaaca
     4861 gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc cggtaccaac
     4921 ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg gcgtgaccac
     4981 gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct ggctggaggt
     5041 cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg gtaatttgaa
     5101 tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag tacagatcca
     5161 gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg ttgcgcgtct
     5221 gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg gagatttttc
     5281 atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt tgggaatgtg
     5341 ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg tacgacagat
     5401 gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga tatcgtccgt
     5461 gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca gcgggttaac
     5521 gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt agcggaatac
     5581 ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg tattcgcatg
     5641 gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa cgccggatcg
     5701 ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt gctggaatcc
     5761 gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa agtcagcgtg
     5821 aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa acagatcgat
     5881 cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc agtaaaatcc
     5941 gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg cgtatcgctg
     6001 gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc gctgcgtatc
     6061 cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga atttgatgtt
     6121 atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc gatggacgtt
     6181 tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac actcggcgtc
     6241 accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga ccgtctggac
     6301 aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag tcagctggac
     6361 gaagcgcgtc gaattgacgt tcagcaggtt gaaaaataat aacgtgatgg gaagcgcctc
     6421 gcttcccgtg tatgattgaa cccgcatggc tcccgaaaca ttgagggaag cgttgagggt
     6481 tcatttttat attcagaaag agaataaacg tggcaaaaaa cattcaagcc attcgcggca
     6541 tgaacgatta cctgcctggc gaaacggcca tctggcagcg cattgaaggc acactgaaaa
     6601 acgtgctcgg cagctacggt tacagtgaaa tccgcttgcc gattgtagag cagaccccgc
     6661 tattcaaacg tgcgattggt gaagtcaccg acgtggttga aaaagagatg tacacctttg
     6721 aggatcgcaa tggcgacagc ctgactctgc gccctgaagg gacggcgggc tgtgtacgcg
     6781 ccggcatcga gcatggtctt ctgtacaatc aggaacagcg tctgtggtat atcgggccga
     6841 tgttccgtca cgagcgtccg cagaaagggc gttatcgtca gttccatcag ttgggctgcg
     6901 aagttttcgg tctgcaaggt ccggatatcg acgctgaact gattatgctc actgcccgct
     6961 ggtggcgtgc gctgggtatc ttcgaacacg taactcttga gctgaattct atcggttcgc
     7021 tggaagcacg cgccaattac cgcgatgcgc tggtggcatt ccttgagcag cataaagaaa
     7081 agctggacga agactgcaaa cgccgcatgt acactaaccc gctgcgcgtg ctggattcaa
     7141 aaaatccgga agtgcaggcg cttctcaacg acgctccggc attaggtgat tatctggacg
     7201 aggaatctcg tgagcatttt gccggtctgt gcaaactgct ggagagcgcg gggatcgctt
     7261 acaccgtaaa ccagcgtctg gtgcgtggtc tggattacta taaccgtacc gttttcgagt
     7321 gggtgactaa cagtctcggc tcccagggca ccgtgtgtgc aggcggtcgt tatgacggtc
     7381 ttgtggaaca actgggcggt cgtgcaacac cggctgtcgg ttttgcgatg ggcctcgaac
     7441 gtcttgtatt gttagtacag gccgttaatc cggaatttaa agccgatcct gttgtcgata
     7501 tatacctggt ggcttcaggt gctgatacac aatctgcggc tatggcatta gctgagcgtc
     7561 tgcgtgatga attactgggc gtgaaattga tgaccaacca cggcggcggc aactttaaga
     7621 aacagtttgc ccgtgctgat aaatggggtg cccgcgttgc tgtggtgctg ggtgagtctg
     7681 aagtggctaa cggcacagca gtagtgaagg atttgcgctc tggtgagcaa acggcagttg
     7741 cgcaggatag cgtagccgcg catttgcgca cgttactggg ttaaggaagg agaaggacag
     7801 cgtggaaatt tacgagaacg aaaacgacca ggtagaagcg gttaaacgct tttttgctga
     7861 aaatggcaaa gcactggctg ttggggtgat tttgggcgtt ggcgcactga ttggctggcg
     7921 ctactggaac agccatcagg ttgattctgc acgctccgct tctcttgcct atcaaaatgc
     7981 ggttaccgca gtgagcgaag gcaaaccgga tagcatcccg gcggcggaaa aatttgctgc
     8041 tgaaaataaa aatacttatg gtgcgctggc ttctttggaa cttgcgcagc aatttgttga
     8101 caaaaatgaa ctagagaaag ctgccgccca gttacaacag gggctggcag acacgagcga
     8161 tgaaaatctc aaagccgtga taaatctgcg tcttgctcgc gttcaggtac agctcaagca
     8221 ggctgatgcc gcgctgaaaa cccttgatac catcaaaggt gaagggtggg ctgccattgt
     8281 tgccgacctg cgtggtgaag cattgctgag caaaggtgat aagcaaggtg cgcgtagtgc
     8341 atgggaagca ggcgtgaaaa gcgatgttac tccggcactg agcgaaatga tgcagatgaa
     8401 aattaataat ttgtccatct gagagggacc cgatgcaatt gcgtaaatta ctgctgccag
     8461 gactgctttc cgttaccctt ttaagcggct gttcgctgtt taacagcgaa gaagatgtgg
     8521 taaagatgtc cccattgcca accgttgaaa accagtttac gccgaccacg gcgtggagca
     8581 cttccgttgg tagcggcatt ggcaacttct attccaatct tcatccggca ctggcggaca
     8641 acgttgtcta tgcagcggac cgcgctggtt tggtaaaagc gctgaatgcg gatgatggca
     8701 aagaaatctg gtctgtcagc ctggccgaga aagatggctg gttctctaaa gagcctgcat
     8761 tactttctgg cggtgtgacc gtgtctggtg ggcatgtcta cattggcagc gaaaaggcgc
     8821 aggtttacgc gctgaatacc agcgatggta ctgtggcatg gcaaactaca gtcgcgggtg
     8881 aagcactttc gcgcccggtg gtcagcgacg gtctggtgtt aatccacacc agtaacggtc
     8941 agttacaagc gctgaacgaa gctgacggcg ctgtcaaatg gacagttaac ctcgatatgc
     9001 cttcgctctc tttgcgtggc gagtctgcgc cggcaacggc ttttggtgcg gctgtcgtgg
     9061 gtggcgataa tggtcgcgtc agcgcagtgc tgatggaaca gggccagatg atttggcagc
     9121 agcgtatttc ccaggcgacc ggttctaccg aaattgaccg tctgagcgat gttgacacga
     9181 ctcccgtcgt tgttaacggc gttgttttcg cgctggccta taatggtaac ctgacggcgc
     9241 ttgatctgcg cagtggtcag attatgtgga aacgcgaact gggttcggtg aatgatttca
     9301 tcgtcgatgg caatcgcatc tatctggtcg atcaaaatga ccgggtgatg gcgttgacca
     9361 ttgatggcgg cgttacgctg tgggcacaaa gcgatctgct gcatcgcctg ctgacttctc
     9421 cggtgctgta taatggcaac ctggtggtcg gtgacagtga aggttatctg cactggatta
     9481 acgtcgaaga tggtcgtttc gttgcccagc aaaaagttga tagttccggt ttccagactg
     9541 aaccggttgc cgctgacggc aaactgctga tccaggcaaa agacggaacc gtgtactcta
     9601 ttacacgtta atcgtctctg tcgttcactt tgaaaacggc tcctggacag gggccgtttt
     9661 cctgttttta acaacgacgc gaatatagcg tgcgttgtct gatgatttat aaaaatgagg
     9721 ctttaaacat ggtacctgtg gtcgcgcttg tcgggcgccc taacgtagga aaatccacgt
     9781 tatttaaccg tctaactcgc acccgagatg cgctggttgc ggatttcccg ggtctgactc
     9841 gtgaccgtaa gtacggtcgt gcggaaattg aaggccgtga gtttatctgt attgataccg
     9901 gcgggattga tggcacagaa gacggtgtag aaacccgcat ggcggaacag tcgctgctgg
     9961 cgattgaaga agcggacgtc gtactgttta tggtggatgc gcgcgcgggc ctgatgccgg
    10021 cagatgaagc gattgccaaa catctgcgct cccgtgaaaa accgaccttc ctggtggcaa
    10081 acaaaactga cggtctggat cccgatcagg cagtggttga tttctactcg cttggtttag
    10141 gtgaaatcta cccgatcgcc gcgtctcacg gtcgtggcgt attaagtctg ctggagcatg
    10201 tgctgctgcc gtggatggaa gatctcgcac cgcaagagga agtcgacgaa gacgctgaat
    10261 actgggcgca atttgaagcg gaagagaacg gcgaagaaga agaggaagac gacttcgacc
    10321 cgcaaagtct gccgatcaaa ctggcgattg tgggtcgtcc gaacgtaggt aagtctacac
    10381 tcactaaccg tattcttgga gaagagcgcg ttgttgttta cgacatgcct ggcacgacgc
    10441 gtgacagcat ctacatccca atggaacgcg atggacgtga gtatgtgctc attgacaccg
    10501 ctggcgtacg taaacgcggc aaaatcaccg atgctgtaga gaaattctcc gtaatcaaaa
    10561 cgttgcaggc cattgaagac gccaacgtgg tgatgttagt gattgatgcg cgcgaaggta
    10621 tttccgatca ggatctctcg ctgctgggct ttattctcaa tagtgggcgc tcacttgtca
    10681 ttgtggtgaa taagtgggat ggcctgagcc aggaagtgaa agagcaggtg aaagaaacgc
    10741 tggacttccg tctgggcttt atcgattttg ctcgtgtgca ctttatctct gccttgcacg
    10801 gcagtggtgt tggtaacttg tttgaatcag tacgtgaagc gtatgacagc tccacccgtc
    10861 gtgtggggac ctctatgctg acgcgcatca tgacgatggc tgttgaagat caccaaccgc
    10921 cgctggtacg cggtcgtcgt gtgaagctga aatatgccca cgctggtggt tataacccgc
    10981 cgattgtggt gattcacggt aatcaggtga aagacctgcc tgattcctac aagcgctact
    11041 tgatgaacta cttccgcaaa tcgctggacg taatgggatc gccgattcgt attcagttca
    11101 aagaagggga aaacccgtat gcgaacaagc gtaacaccct gacgccaacc cagatgcgta
    11161 aacgtaagcg tctgatgaag cacatcaaga aaaataaata attatccgta agtattttat
    11221 ctgccattcc ctctatgaat gtagagggaa tggctaccgg aggaattcat atggaactgc
    11281 attgtccaaa atgtcagcat gtgcttgatc aggataacgg ccatgcgcgt tgcccatcct
    11341 gcggggaatt tattgaaatg aaagctcttt gtccagactg ccatcaaccg ttgcaggtgt
    11401 taaaagcctg cggtgcagta gattacttct gtcagcacgg gcatggcttg atatcgaaaa
    11461 aacgcgttga attcgtgctg gcttaatgaa cctttttacg cgattttttt accggctgga
    11521 tgttttttac ttcactttct atccagccgt cttccagacg tgtggttagc atttcacccg
    11581 ctttaacttg cttcactttt ttcagtacat tgccgtcagt agcagtagta acgctgtatc
    11641 cacgcgccag cgttgacagt gggcttacgg cttcgaggtg cgttactgca ttaccgaaac
    11701 gttcacgcgt ggcgctaagc tgtgcgcgca ggatttctgc taaacgatat tccagttgct
    11761 gaatgcgcgt ttgcgtgcga tgaatcttcg gctgtggatt ttgctgattc agccgctgtg
    11821 ttaatcgctg ctgctgttgc ccggcacgct taagctgatt ttccagcgca aagctcatcc
    11881 gtttttgcag gcgttcaagc atggtttgct ggcgtgccag ccggagctgc ggatgctgct
    11941 gctgcaagcg atgatgaatc tgcgtaaagc gacgcgtacg gttggcgaga taataatcca
    12001 tcgccatctc cagccgttga cgggccgatt gcacctggcg cagtaactct tgctgattac
    12061 ggctcactac ttcagcggcg gcagacggcg ttggcgcacg cagatcggca acaaaatcgg
    12121 caatggtcac atccgtctca tgcccgacgg cgctgacgac cggaatgcgg ctggcaaaaa
    12181 tcgcccgcgc tacgcgttcg tcgttaaaac tccataaatc ttccagcgaa ccgccgccgc
    12241 gcccaacgat caacacgtcg cactcattgc gctgattcgc cagttcaatg gcgcgaacaa
    12301 tttgccccgg cgcgtcatcg ccctgaacgg aggtggggta gatgatcacc ggtagagaag
    12361 gatcgcgacg ttttaacaca tgcaaaatat catgtagcgc agcaccggtt tttgaggtga
    12421 tcacaccaac gcaatgcgca ggggagggaa gtggtttttt gtattgctga tcgaacaaac
    12481 cttcagcctg caacttcgct ttgagctgtt cgtacttctg ttgcagcagc ccttcaccgg
    12541 ccggctgcat actctcaacg attatctggt agtcgccgcg cggctcgtag agcgtaatat
    12601 tggcgcgaac taaaacttgt tgcccatgct gcggacggaa ggtgacccgg cggttgctgt
    12661 tgcggaacat cgcgcagcgt acctgggcgg tgtcgtcttt gagtgtaaag taccagtgac
    12721 cggaagccgg ttgtgtgaaa ttagagattt cgccgctgat ccaaacctgt cccatctcat
    12781 gctcaagcag cagacgaacc gtttgattca ggcgactaac ggtaaaaatt gcaggggatt
    12841 gagaaggtaa catgtgagcg agatcaaatt ctaaatcagc aggttattca gtcgatagta
    12901 acccgccctt cggggatagc aagcattttt tgcaaaaagg ggtagatgca atcggttacg
    12961 ctctgtataa tgccgcggca atatttatta accactctgg tcgagatatt gcccatgcta
    13021 cgtatcgcta aagaagctct gacgtttgac gacgttctcc tcgttcctgc tcattctacc
    13081 gttctgccga atactgctga cctcagcacc cagctgacga aaactattcg tctgaatatc
    13141 cctatgcttt ccgcagcaat ggataccgta acggaagcgc gcctggctat tgctctggct
    13201 caggaaggcg gtatcggctt tatccacaaa aacatgtcca ttgaacgcca ggcagaagaa
    13261 gttcgccgtg tgaaaaaaca cgaatctggt gtggtgactg atccgcagac tgttctgcca
    13321 accacgacgc tgcgcgaagt gaaagaactg accgagcgta acggttttgc gggctatccg
    13381 gtcgttaccg aagaaaacga actggtgggt attatcaccg gtcgtgacgt gcgttttgtt
    13441 accgacctga accagccggt tagcgtttac atgacgccga aagagcgtct ggtcaccgtg
    13501 cgtgaaggtg aagcccgtga agtggtgctg gcaaaaatgc acgaaaaacg cgttgaaaaa
    13561 gcgctggtgg ttgatgacga attccacctg atcggcatga tcaccgtgaa agacttccag
    13621 aaagcggaac gtaaaccgaa cgcctgtaaa gacgagcaag gccgtctgcg tgttggtgct
    13681 gcagttggcg caggtgcggg taacgaagag cgtgttgacg cgctggttgc cgctggcgtt
    13741 gacgttctgc tgatcgactc ctcccacggt cactcagaag gcgttttgca gcgtatccgt
    13801 gaaacccgtg ctaaatatcc ggatctgcaa atcatcggcg gcaacgtggc aacagctgca
    13861 ggtgcacgcg ctttggcaga agctggttgc agcgcggtta aagtcggtat cggccctggt
    13921 tctatctgta cgactcgtat cgttactggc gtaggtgttc cgcagatcac tgccgttgct
    13981 gacgcagttg aagccctgga aggcaccggt attccggtta tcgcagatgg tggtattcgc
    14041 ttctccggcg acatcgccaa agctatcgcc gctggcgcaa gcgcggtgat ggtaggttcc
    14101 atgctggcgg gtactgaaga atctccgggt gaaatcgaac tctaccaggg ccgttcttat
    14161 aaatcttacc gtggtatggg ttccctgggc gcgatgtcca aaggttcctc tgaccgttat
    14221 ttccagagcg ataacgctgc cgacaaactg gtgccggaag gtatcgaagg tcgcgtagct
    14281 tataaaggtc gcctgaaaga gatcattcac cagcagatgg gcggcctgcg ctcctgtatg
    14341 ggtctgaccg gctgtggtac tatcgacgaa ctgcgtacta aagcggagtt tgtacgtatc
    14401 agcggtgcgg gcattcagga aagccacgtt cacgacgtga ccattactaa agagtccccg
    14461 aactaccgtc tgggctcctg attctcttcg cccgacttca tgtcgggcga tttatattat
    14521 ctgtttcact tgcctcggaa taagcgtcaa tgacggaaaa cattcataag catcgcatcc
    14581 tcattctgga cttcggttct cagtacactc aactggttgc gcgccgcgtg cgtgagctgg
    14641 gtgtttactg cgaactgtgg gcgtgggatg tgacagaagc acaaattcgt gacttcaatc
    14701 caagcggcat tattctttcc ggcggcccgg aaagtactac tgaagaaaac agtccgcgtg
    14761 cgccgcagta tgtctttgaa gcaggcgtac cgatattcgg cgtttgctat ggcatgcaga
    14821 ccatggcaat gcagttgggc ggtcacgttg aagcctctaa cgaacgtgaa tttggctacg
    14881 cgcaggtaga agtcgtaaac gacagcgcac tggttcgcgg tatcgaagat gcgctgaccg
    14941 cagacggtaa accgctgctc gatgtctgga tgagccacgg cgataaagtt accgctatcc
    15001 cgtccgactt cgtcaccgta gccagcaccg aaagctgccc gtttgccatt atggctaacg
    15061 aagaaaaacg cttctatggc gtacagttcc acccggaagt gactcacacc cgccagggta
    15121 tgcgcatgct ggagcgtttt gtgcgtgata tctgccagtg tgaagccctg tggacgccag
    15181 cgaaaattat cgacgatgct gtagctcgca tccgcgagca ggtaggcgac gataaagtca
    15241 tcctcggcct ctctggtggt gtggattcct ccgtaaccgc aatgctgctg caccgtgcta
    15301 tcggtaaaaa cctgacttgc gtattcgtcg acaacggcct gctgcgtctc aacgaagcag
    15361 agcaggttct ggatatgttt ggcgatcact ttggtcttaa cattgttcac gtaccggcag
    15421 aagatcgctt cctgtcagcg ctggctggcg aaaacgatcc ggaagcaaaa cgtaaaatca
    15481 tcgggcgcgt tttcgttgaa gtattcgatg aagaagcgca gaaactggaa gacgtgaagt
    15541 ggctggcgca gggcaccatc taccctgacg ttatcgaatc tgcggcttct gcaaccggta
    15601 aagcacacgt catcaaatct caccacaacg tgggcggcct gccgaaagag atgaagatgg
    15661 gcctggttga accgctgaaa gagctgttca aagacgaagt gcgtaagatt ggtctggagc
    15721 tgggcctgcc gtacgacatg ctgtaccgtc atccgttccc gggaccaggc cttggcgttc
    15781 gtgttctggg tgaagtgaag aaagagtact gtgacctgct gcgccgtgct gacgccatct
    15841 tcattgaaga actgcgtaaa gcggacctgt acgacaaagt cagccaggcg ttcaccgtct
    15901 tcctgccagt acgttccgtt ggcgtaatgg gcgatggtcg taagtatgac tgggttgtct
    15961 ctctgcgtgc tgtcgaaacc atcgacttta tgaccgcaca ctgggcgcat ctgccgtacg
    16021 atttcctcgg tcgcgtttcc aaccgcatta tcaatgaagt gaacggtatt tcccgcgtgg
    16081 tg
//
LOCUS       DAJTNM010000085        16069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTNM010000085 DAJTNM010000000
VERSION     DAJTNM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.085"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(272..976)
                     /locus_tag="OO172_002811"
     CDS             complement(272..976)
                     /locus_tag="OO172_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5512933.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1113..1565)
                     /gene="moaE"
                     /locus_tag="OO172_002812"
     CDS             complement(1113..1565)
                     /gene="moaE"
                     /locus_tag="OO172_002812"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5512934.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1567..1812)
                     /gene="moaD"
                     /locus_tag="OO172_002813"
     CDS             complement(1567..1812)
                     /gene="moaD"
                     /locus_tag="OO172_002813"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5512935.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1805..2290)
                     /gene="moaC"
                     /locus_tag="OO172_002814"
     CDS             complement(1805..2290)
                     /gene="moaC"
                     /locus_tag="OO172_002814"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5512936.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2293..2805)
                     /gene="moaB"
                     /locus_tag="OO172_002815"
     CDS             complement(2293..2805)
                     /gene="moaB"
                     /locus_tag="OO172_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5512937.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2827..3816)
                     /gene="moaA"
                     /locus_tag="OO172_002816"
     CDS             complement(2827..3816)
                     /gene="moaA"
                     /locus_tag="OO172_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5512938.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3804..3948)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4213..5121
                     /gene="yvcK"
                     /locus_tag="OO172_002817"
     CDS             4213..5121
                     /gene="yvcK"
                     /locus_tag="OO172_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5512939.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5313..7334)
                     /gene="uvrB"
                     /locus_tag="OO172_002818"
     CDS             complement(5313..7334)
                     /gene="uvrB"
                     /locus_tag="OO172_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5512940.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7913..8590)
                     /gene="bioD"
                     /locus_tag="OO172_002819"
     CDS             complement(7913..8590)
                     /gene="bioD"
                     /locus_tag="OO172_002819"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5512941.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8583..9338)
                     /gene="bioC"
                     /locus_tag="OO172_002820"
     CDS             complement(8583..9338)
                     /gene="bioC"
                     /locus_tag="OO172_002820"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5512942.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9325..10479)
                     /gene="bioF"
                     /locus_tag="OO172_002821"
     CDS             complement(9325..10479)
                     /gene="bioF"
                     /locus_tag="OO172_002821"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5512943.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10476..11516)
                     /gene="bioB"
                     /locus_tag="OO172_002822"
     CDS             complement(10476..11516)
                     /gene="bioB"
                     /locus_tag="OO172_002822"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5512944.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11603..12892
                     /gene="bioA"
                     /locus_tag="OO172_002823"
     CDS             11603..12892
                     /gene="bioA"
                     /locus_tag="OO172_002823"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5512945.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12870..13340
                     /locus_tag="OO172_002824"
     CDS             12870..13340
                     /locus_tag="OO172_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5512946.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13894..14895
                     /locus_tag="OO172_002825"
     CDS             13894..14895
                     /locus_tag="OO172_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5512947.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14929..>16069)
                     /locus_tag="OO172_002826"
     CDS             complement(14929..>16069)
                     /locus_tag="OO172_002826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR5512948.1"
                     /translation="YDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFD
                     NVMVNEVETAYLMQGLWHSKFIACQAGTCRVGLHFLGQCVSVSVSSCHFSRGNYSADE
                     SFGIRIQPQTYAWSSEAVRSEAIILDSETMCIGFKNAVYVHDCLDLHMEQLDLDYCGS
                     TGVVIENVNGGFSFSNSWIAADADGTEQFTGIYFRTPTSTQSHKIVSGVHINTANKNT
                     AANNQSIAIEQSAIFVFVSGCTLTGDEWAVNIVDINECVSFDKCIFNKPLRYLRSGGV
                     SVTDCYLAGITEVQKPEGRYNTYRGCSGVPSVNGIINVPVAVGATSGSAAIPNPGNLT
                     YRVRSLFGDPASSGDKVSVSVVTINVTRPSPVGVALPSMVEYLAI"
BASE COUNT         3818 a         4231 c         3997 g         4023 t
ORIGIN      
        1 taccgtttaa tatcctggcc tcgtgctgaa aatctaattt attgctgttt tgcgagtatg
       61 attccattct tttagttgtc cttagaaaaa atattaatat tcaccatcaa taaatttaaa
      121 accacgcata caaaatactt ttgtttttat ggatacggaa aacgattccg ctcgctcata
      181 aggcatattt tatacctggt acttgttaag tgtcgttttt agtaagaaaa aaatcccgac
      241 acatggccgg gatagtgatg gtgaaaaaga attaacggcg gttgccgaag atccgtaaca
      301 acatcaggaa caggttgatg aagtccagat acaaggttaa cgcgccaaga atggaatatt
      361 tgcgcaggtt cgacgtgtcg cgggtatcaa tctgctcacc catatttttc aatttctgag
      421 tgtcatacgc cgtcaatccg acaaagacaa tcacgccgat gtaggtaact gcccacatca
      481 atgcttcgct tttcagccag aagttgacca gcgatgccag cacaatgccg attaacgcca
      541 taaacagcat attgcccaag ccacttaaat cgcgcttcgt ggtgtaaccg tacaggctca
      601 ttgcgccgaa catcccggca gtaacgacga aagtactggc gatagaagca gcggtataga
      661 caatgaatat actggaaagc gtaagacccg tcagcgccga ataaagcata aagagcatcg
      721 tcgttacacc tgcgctcagc ttttgaatca tcgctgataa cacaataacc aatgctaatt
      781 gcgcgatgat cagaccgatt aaaaagacac ggttagtgaa caacagctcc atcacggccg
      841 cggaattagc cgcataccag gcaacaaatg cggtcagcaa caagccaacg gtcatccagc
      901 catagacttg cgccatataa gtttgcaagc cagcccgggg ttgtacgatt gaatcagaac
      961 gtgggaatct gtccatgacg atctcctgaa gatataagga atatcttaag gatactgcaa
     1021 aatgatgagg ctgtgcatcg acgcagcgta aacgcatgta ctgagcggtg aaattgccgg
     1081 acgcagcggt gccttatccg gctaacaaaa aactaccagc gttttgccgc ctgcagatcg
     1141 ctctcccggg cttcaaccca gcggtcgcct tccggcgtgg cttcgcgctt ccagaacggt
     1201 gcgcgggttt tgagataatc cataataaac tgcccggctt caaacgcact gctgcgatgc
     1261 gcactggtga caccgacaaa aacgatttca tcgcccggcc ataattcccc gatgcggtga
     1321 atcaccgtga cgcgccccag cggccagcgg ttacgcgctt catcaacaat ttctgccagt
     1381 gctttttcag tcatccccgg atagtgttcg agggttaatg ctttgacgct gtcgcccagg
     1441 ttatggttgc gcacctttcc ggtgaaggtg actaccgcac cgtcttcgtc acgctccgcc
     1501 agccacgggt actcttctcc tacgctgaac ggctgcgggc cgacaacaat tttggtttct
     1561 gccatcttaa cctccggtta ccggcgggag gaaagctact tcgtcgccgt cagtcagcgg
     1621 atggtcaaaa ctcaccagcg tctggttgac ggcagccagc aatttgccat cttccagcgc
     1681 cagcgcccag cgatcgctct gcgcagccag gtgctggcgt aacgcttcaa cggttgggta
     1741 atccgcagcc acttcggttg catctgttcc caccaactcg cgcacctggg cgaaaaaaag
     1801 aactttaatc atccgcttcc accttaaagt cacccgactt gccgccgctc ttcgccagca
     1861 aacgtaccgg accaatcacc atatcttttt gcaccgcttt gcacatgtca taaatggtca
     1921 gcgccgccac ggaggccgcg gttaacgctt ccatttcgac accggttttc ccggtcaggc
     1981 ggcataaggt ttctatacgc acccgattgt gctccggctc ggcctgtaaa ttgacttcaa
     2041 ctttgctgag catcagcggg tgacacagcg ggatcagatc ccaagtgcgt tttgccgcct
     2101 gaataccggc aatacgcgca gtggcaaata cgtcgccttt gtggtggcga ccatcaataa
     2161 tcatcgccag cgtctcgctg cgcatggtga caaaggcttc cgcccgcgct tcacgcacgg
     2221 tttccgcttt ggcggagaca tccaccatgt gcgcttcgcc agcggcgttg atatgggtca
     2281 gttgcgacat acttatttct tcaaatgtgg atggaaatta cacggacgcg tacgggcatc
     2341 cagctgcggc gcgatgatat tttcccatgc ggtacggcac gctttggtcg aacccggcat
     2401 ggcgaaaatc agcgttttgt tagcgacgcc cgctaccgca cgagattgca acgtggaagt
     2461 gccaatctct tcaaacgaca acatacggaa cacttcacca aaaccttcaa cctcacggtc
     2521 gaacaacggc agcagcgctt cgggagcctg atcaccttcc gtcaggccag taccacccgt
     2581 aatcaatacc acttgtacat cgtcgctggc gatccacgca gatacctgag cgcgaatagc
     2641 gtagcggttt tctttcacaa tggctttatc gacaacgtga tggcccgctt cttgcgccga
     2701 atcgcgcaga tagtgaccgg aggtatcgtc ttcttcaccg cgacgattag aaaccgtaag
     2761 aatagcaata cgggtcggga taaattcagt gcttacctga ctcatctgat ctctcctttt
     2821 gacgttttag ccgccaatgt acgataagtt ttgcgtaata ccggtgttgt tttgatgcag
     2881 gaaatgggtc tgtttcttct cccgcagcgc cgctgaaata cgcgcttcca gcgcctgttg
     2941 ctgggtatcg tcttccagca gatcgcgcag gttaacgccg ccttcaccaa acaggcagag
     3001 atggagttta ccaatggagg aaacgcgcag gcggttgcaa gtggcgcaga agtctttttc
     3061 atacggcatg ataaggccaa tctctccggc gtaatctgga tggcaaaaga cttgcgcggg
     3121 accgtcgctg cgttgacgta attggtggat ccagccgcga cgcagtagct cgtcacgcag
     3181 aacctgacca gagatgtgat gcttacggaa gagctcgctg ccctcgcccg tttccatcag
     3241 ttcgatgaaa cgcagctgga tagggcgatg ctggatccag ttcagaaagg tgtcgagctg
     3301 gtgatgatta acatcacgca tcagcacggt attgactttg accttctcaa aaccggcctc
     3361 aaatgcagca tcaatccctg ccatgacctg gttgaattta tcctgcccgg taatagcgtg
     3421 aaactggcgg gcgtccaaac tgtcgacact gacgttaatg ccagtaagtc ccgcatcgcg
     3481 ccagttcgcc acatcgcgtt ccagacggta accattggtg gtgaccgcaa tctggcggat
     3541 agcgtcgttt tcccgcacag cggcgatgat atcggtaaag tcgcggcgta aagacggctc
     3601 tcctcctgtc aggcgcactt tttcggtgcc caggctggcg aaggcgcgcg taacccggcg
     3661 aatttcatcg acggtaagaa agcctttatt ggtgacgccg ctcggtttgt agccatccgg
     3721 caggcagtag gtgcaacgaa agttacacac atcggtaatc gacaggcgca agtagtaaaa
     3781 cttacgcgca aatgcatcag tcagttgtga agccatgtac acctttccag atacgggagg
     3841 cgaagtcatt tcttccttcg ccctggtggc aatctttacg agcattgtca cggccaaagc
     3901 accgtatcag ttgacccagg tgcagaggct agagtgttta gtggttaagc cgatactagc
     3961 gtgtaaatga gatttttacc atccacactt tcgctatgca atcatgtata tagcgtcatg
     4021 atcgtgctat ttcgctacag aataagggaa aatggcggtt ttactttgat atacatcatc
     4081 agggaggcat agcgtgcatc gtcctttaga gatagaagaa aatagtttgt atcctcaata
     4141 ttggcaggtt aattgctgtt tccccgcaat ttgcgctact gtagcgcgga taatctgact
     4201 ccaggaaact atatgcgcaa tcgtacgctg gctgatcttg atcgtgtcgt tgctctcggc
     4261 ggagggcatg gactgggacg cgttctctca tcactttcgt ctttgggttc tcgtttaacg
     4321 ggtatcgtca ccaccacgga taatggtggc tcgacggggc gtattcgccg ttcagaaggc
     4381 ggcattgctt ggggcgatat gcgcaactgc ctcaaccagc tgataacgga accgagcgtc
     4441 gcctccgcga tgtttgaata ccgttttggc ggcaatggcg aactttccgg tcataatctc
     4501 ggaaacttga tgttaaaggc gctggatcac cttagcgtgc ggcctctgga agccatcaat
     4561 ttaattcgta atctgctgaa agtggatacg catttgattc caatgtcaga gcatcctgtt
     4621 gatctgatgg cgattgacga tcaggggcat gaagtttacg gcgaggtcaa tatcgaccag
     4681 ttaactacgc cgattcaaga gttattgtta acgcctaatg tacccgcaac gcgtgaggcg
     4741 gttcacgcta tcaatgaagc ggatctcatc attattgggc ctggcagttt ttataccagc
     4801 ctgatgccaa ttctgctgct gaaggaaatc gcccaggcat tacgccgcac gccagcgccg
     4861 atggtttata tcggcaatct ggggcgtgag ttgagtttac ctgcggctaa tttgaagctg
     4921 gaaagcaagc tggcaattat ggagcagtat gttggtaaaa aggtcattga tgcggtcatc
     4981 gtcgggccaa aagtggatgt ctcggcggtg aaagagcgga ttgtgatcca ggaggtactg
     5041 gaggccagcg atattccgta tcgtcatgac cgccagttgt tacataacgc gctggaaaag
     5101 gcgttacagg ctttaggtta acggaaatcc gaaggaaaat tccggcttcc tattgaagac
     5161 aaagtgcgcg ttgtttatgc cggatgcggc gtgaacgcct tatccggcct acaaaccgcg
     5221 caaattcaat atattgcgga gaaaatgtag gcctgataag cgtagcgcat caggctgttt
     5281 tccgtttgtc atcagtcttc ttcgctatcc tgttacgatg cggcaataaa caactcacgc
     5341 aactgatgca actggtcacg aatttgtgcc gcttcttcga actccagatt ctgcgcgtgt
     5401 tgcatcatca gcccttccag ttcatggatt ttctgctgca acgctttcgg cgacatatcc
     5461 atcggcacat tatccggctc aacaatcggg cgcgattttc ctctgccctt cgctttggtt
     5521 ttggcaatgt tctgccccag cgccaggata tcgaccactt tcttgttcaa gccttgcggc
     5581 gtaatgccgt gttcctcgtt gtacttctgc tgtttctcac ggcgacgttc ggtttcgcca
     5641 atcgctttcg ccattgatgg agtgatctta tcgccgtaga gaatcgcttt accgttaacg
     5701 ttacgtgccg cacgaccaat ggtctggatc aacgaacgtt cggaacgcag gaagccttct
     5761 ttgtcagcgt cgaggatcgc caccagcgac acttccggca tatccagacc ttcacgcagt
     5821 aagttgatcc ctaccaatac gtcgaactca cccagacgca agtcgcggat aatctccata
     5881 cgctcgacgg tgtcgatatc tgagtgaaga taacgcacgc gctcaccgtg ttcttcgaga
     5941 tattcggtaa gatcttccgc cattcgcttg gtcagtgtgg tgaccagcac gcgttcgtta
     6001 attgccgctc gctgacgaat ctccgaaaga agatcatcaa cctgtgtcgc caccggccgc
     6061 acttcgataa tcgggtcaag caatccggtt ggacgcacca cctgatccac cacatcgccg
     6121 ccggattttt ccagctcgta attacccggc gtcgccgaaa catagatggt ttgcggcgct
     6181 aatgcttcga actcttcaaa cttaagcgga cggttatcca gcgctgatgg caggcggaag
     6241 ccgtactcca ccagtgtctc tttacgcgcc cggtcaccgc gatacatgcc gccaatttgt
     6301 ggaatggtga cgtgagattc atcgacgacc agcagcccat cggcaggcag gtaatcaaac
     6361 agcgtcggcg gtggctcacc cggtccacga ccggagagga agcgcgagta gttttcaatc
     6421 cccgaacagt agcccagctc gttcatcatc tccagatcaa actgggtacg ctgggtcagc
     6481 cgctgctctt ccagcagttt gttgttttcc aacagcactt tgcgtctggc ggccagttct
     6541 tctttgatct cctccatcgc ctgtacgatg cgctcgcgcg gtgtgacgta gtgcgttttc
     6601 gggtagatgg taaaacgtgg aatagtggaa acaatctgcc cggtcagcgg gtcaaataac
     6661 gacaatcgtt ccacttcctc gtcaaacagt tccacgcgaa gtgcaatgtc atccgattct
     6721 gccgggaaga tatctatcac ctcgccacga acgcggaaag taccacgctg gaatgcttga
     6781 tcattacgag cgtattgcag ctccgccagt cggcgcagaa tcgcgcgctg atcgataatc
     6841 ataccgaccg tgagatggag catcatcttg agatataaat caggatcgcc cagaccataa
     6901 atcgcggaaa cagacgccac tacaatcaca tcacgccgct ccagcatcgc tttggtggcg
     6961 gacaaacgca tctgctcaat atgttcgtta accgaggcat ctttctcaat gaaggtgtcg
     7021 gaactcggta cataggcttc cggctgatag tagtcgtagt aggagacgaa atactccacc
     7081 gcgttttccg ggaagaactc tttcatttcg ccatacagct gggccgccag cgttttgttg
     7141 ggcgcaagta ccatggttgg gcgctgaagg tcagcaatga cattggcaat ggtgaaggtt
     7201 ttcccggagc cagtcacgcc aagtaacgtc tggtgcgcca ggccatcttc cagcccctct
     7261 tcgagacgtc gaatcgcctc tggctgatcg ccagaaggtt taaaagcgga attcagtttg
     7321 aacggtttac tcatgagtcg ctacctgaag gagttgggcg ggcaggtatg taattttact
     7381 cgtcgtactt aattatgcca acaaattata ctggataaaa aaacagttca tcaccataat
     7441 atttctgata cagcgtaaac tccgctgtca atcatgagca aaatttactc tgtggcgaga
     7501 taaaactccg gccttaccgg gttatcccca aagcaacggc ttttttaaca tttgtcaaga
     7561 tgagtgatga cagttttatg gcaagagatg cctgtacagt gactgccatt gcatttatct
     7621 aaccagttaa aaaataaaag atatttcttt gagccgtctt taacgccaat tcgcgtgcaa
     7681 gccgcgtatt ctctcgcttg cctcgtgttt tctaactcta atacacatgg ttatccacag
     7741 gaatagtgga taactgtctc cagcccctat cccccgccgc ctgggcaatt cccacattcg
     7801 ccgcatgagg cggaagtaaa atttttttcg tgattatttt agaatttaat tggttaaatt
     7861 gcagtcaatc gaagacgcaa tctcgctcgc aatttaacca aatacagaat ggctacaaca
     7921 aggcaaggtt tatgtacttt ccggttgccg cattttctgg attttctgca agccagggga
     7981 tctctcccag cagcggcgcg ggaatcatgc gggtgagcgt ggtcatatat tcagcgtgac
     8041 gttttcccgg aggcgtaaca tcgttcgcca cccaacccgc cagagtcagt ccggcgtgtt
     8101 gtattacctg cgcagtcaac atcgcgtgat taatacagcc gagtttcaca ccaactacca
     8161 gtatcaccgg cagttgttcc tgtgttaccc aatctgcaaa agtgaaagtg tcagaaagcg
     8221 gcgtaaacca gccgccagca ccttccacta acacccagtc agcctgttgt tcaagcgcgc
     8281 gtaatccggc gctcattacc aatgattcta tcggcctgcc ctcttgggcg ctgatgatgt
     8341 gcggcgaggt gggttctgcg aaggtgtaag gatttaccgt tgcgtaatcc agctgcaggc
     8401 tgctgttgtg ctgtaacgcc agcgcgtcac tattacgtaa gccttccggg gtcttttcgc
     8461 tgccagaggc gaccggttta taacctgccg tccgataacc tactgccttc gcggcttgta
     8521 aaagtgcaca actggcgacg gttttcccca cttcggtatc cgttccggtg acaaaataac
     8581 gtttactcac gagcaatcac tcccaaaaaa agatgatacg tcagaggata tcgcccctgt
     8641 tgttgcggcc aggccagttg caatcgctgc aactgcgaac gcgttaatat tcgcgggtcg
     8701 cgcccttcat gaagatgcgt agcaccgatg cctttcagcg aacgcatggc actgagcgca
     8761 tcatcaaacc atagtgtgat gagctgaata tgatgttgat aacgcacgcc gttcagcgac
     8821 tcttcgattt catctggcgg taaaaagcga ttagcatgcg gacgctcgtc caccgcctgc
     8881 cgcgcctgat gcagttcggg taacgatccc tgcaccagcg tggtaaacgc gaccacgcct
     8941 ccggggcgca ccacccgata cagctcgcgg agtgccgtgg ataaattacc gcaccactgc
     9001 accgcgagat tgctccatgc aagatcgaac gtcgcagtcg ctaacggcag ggattcgata
     9061 tctcccgcca gataatgatc tgcggcatct ttatggcggg cttgcgcaag cattggcggc
     9121 gagagatcta acgccgtcac ctgcgcgtga cgttcccgcc agtggcggct catccagcca
     9181 ggtccacaac ccgcgtccag tacgtgggtg tatttacgct gtggaagcat tgccagtaag
     9241 gcgtcagcac tctggcgctg tagatctgca tgttgctcat agtgtgcggc tgcccgacca
     9301 aatgccgctg caatggcttg tttattaacc gttgccatgc agcacctcca gcagacggtc
     9361 gatatcctgc atttcatgcg cagcggttag cgttaagcga agtcgcgcag taccagcggg
     9421 tacggttggc gggcgaatcg ccgtgaccca gcagccttgc tggcgcagtt tttctgccag
     9481 ttgtaacgca cggctgttat caccgacgat caatggctgg atggcgctgc atgaatcagc
     9541 cagcgtaaac ggcaaatcct gtactccggc acgaaaacgc gcaatgagtg acaccagttt
     9601 ttcgcgccgt gcatccccct catcacggcg aatgaccgcc agcgacgcac gtaatgcctg
     9661 cgcctgagcg ggcggcatac tggtgctgta gataaggtgg cgggcgaatt gcagcagata
     9721 atccgccacc gtacttgagc aaagcaccgc agccccactg acgccaaatc ctttgccaaa
     9781 agtcactacc agcaattctg gttttacctt ttgcagccag cagctgccgc gcccctgctc
     9841 cccgataacg cccgtgccgt gggcatcatc gaccatcaac cagccattgt gctgttgcgt
     9901 tacctgctgg atttccgcca gtggcgcgct atcgccgtcc atgctgaaca cgccttctgt
     9961 caccaccagt tgctgccccg gacaggggga agcaagcagt cgcgccagat gagcgacatc
    10021 gttatgagta aaacggcgaa gctgcgacgg gcttaaactg gcagcctcca gcaatgaggc
    10081 atggctaagc cggtcggcaa caatacggtc ctctttcgcc atcatcgcgg caatgactgc
    10141 ctgattcgcg gcaaaaccag agataaacag cagcgcccgc gaatagccaa gccactcggc
    10201 cagttcttct tccagcgcct gatgcaccac gctataaccg ctgacgtgac cggagccgcc
    10261 gctaccgatg ccaaattgct ccgccccctg cttccaggca cggataattt gcggatgatg
    10321 gcttaaacct aaataatcgt tactggaaaa gttcagatac tggcgatcat ccgccaccag
    10381 ccagcgtccg gctccttgcg ccaccggata acggcgacgc agggcatcgg cagcacgccg
    10441 cgcatcgagc gccgcgttga ttttctcctg ccagctcata atgctgccgc gttgtaatat
    10501 tcgtcggtgt ccggggtcat cagcgcctgt ccaagacgtt gctgttgttc gttatcccct
    10561 gccagcacgg cagtttgctg cggatttagc cccagtttgc ggaacagttg caggtcttta
    10621 tcttcttccg gattcggcgt ggtcagcagt ttgcaaccgt agaaaatcga gtttgcgcct
    10681 gccataaagc acatcgcctg agtctgttcg ttcatctgct cgcgtccggc agaaaggcgc
    10741 acgtaagagg tcggcatcat gatccgcgct acggcaatgg tgcgaataaa atcaaaggca
    10801 tcaacgtcat cgttatcggc aagcggcgtg cctttcacct tcaccagcat gttgattggt
    10861 acgctttccg gcggcgtcgg caggtttgcc agttgcagca ataatccggc gcgatctttt
    10921 accgtttcgc ctaagcccac aatgccacct gagcagactt tgatcccggc atcgcgcact
    10981 ttttccagcg tatcgaggcg ttcctgataa gtgcgtgtgg tgatgatatt gccgtaaaac
    11041 tccggcgagg tgtccaggtt gtggttgtag taatccagcc cggcgttcgc gaggcgctgc
    11101 gcctgagatt cactcaacgt gcccagcgtc atacacgcct ccagccccat cgcttttacc
    11161 ccctgcacca tttgttccag gtacggcata tcgcgttcgt ggggattctt ccatgccgcg
    11221 cccatacaga agcgcgtcga tcctgccgct ttcgctttgc gcgccgactc cagcacctgt
    11281 tcaacttcca tcaaccgctc ggcttccagc ccggttttgt agcgcgagct ttgcgggcag
    11341 tatttgcaat cttccggaca agctccggtc ttaatcgaca gcaacgtgct gacctgcacc
    11401 tgacgaggat cgaaatgctg gcgatgcacc tgctgcgctt caaacagcag atccagcaac
    11461 ggtttttcaa ataattctgt gacttgcgac aatgtccagc gtgggcggtg agccatgggg
    11521 cttctccaaa acgtgttttt tgttgttaat tcggtgtagg cttgtaaacc taaatctttt
    11581 caatttggtt tacaagtcga ttatgacaac ggacgatctt gcctttgacc aacgccatat
    11641 ctggcaccca tacacatcca tgacctcccc tctgccggtt tatccggtgg cgagcgccga
    11701 aggttgcgag ctaattttgt ctgacggcag acgcctggtt gacggtatgt cgtcctggtg
    11761 ggcggcgatc cacggctaca atcacccgca gcttaatgcg gcgatgaaga cgcaaattga
    11821 tgccatgtcg catgtgatgt ttggcggtat cacccatgcg cccgccatcg agctatgccg
    11881 caaactggtg gcaatgacgc cgcaaccgct ggagtgcgtt tttctcgcgg actccggttc
    11941 cgtagcggtg gaagtggcga tgaaaatggc gttgcagtac tggcaagcca aaggcgaagc
    12001 gcgccagcgt tttctgacct tccgcaatgg ttatcatggc gatacctttg gcgcgatgtc
    12061 ggtgtgcgat ccggacaact caatgcacag cctgtggaaa ggctatctgc cagaaaactt
    12121 gtttgctccc gccccgcaaa gccgcatgga tggtgaatgg gatgaacgcg atatggttgg
    12181 ttttgcccgc ctgatggcgg cgcatcgtca tgaaatcgca gcggtgatca ttgagccgat
    12241 tgtccagggc gcaggcggga tgcgcatgta ccatccggaa tggttaaaac gaatccgcaa
    12301 aatgtgcgat cgcgaaggta tcttgctgat tgccgacgag atcgccaccg gatttggtcg
    12361 taccggcaaa ctgtttgcct gtgaatatgc agaaatcgcg ccggacattt tgtgcctcgg
    12421 taaagcctta accggcggca caatgaccct ttccgccaca cttaccacgc gcgaggttgc
    12481 agaaaccatc agtaacggcg aagccggctg ctttatgcat gggccaactt ttatgggcaa
    12541 tccgctggcc tgcgcggcag caaacgccag cctggcgatt atcgaatccg gcgaatggca
    12601 gcagcaggtg gcggctattg aagtgcagct gcgcgagcaa ctggcaccag cccgtgatgc
    12661 cgaaatggtt gccgatgtgc gcgtactggg ggcaatcggt gtggtcgaaa ccactcgtcc
    12721 ggtgaatatg gcggcgctgc aaaaattctt tgtcgaacag ggggtatgga tccggccttt
    12781 tggcaaactg atttacctga tgccgcccta tattattctc ccgcaacagt tgcagcgtct
    12841 gaccgcagcg gttaaccgcg cggtacagga tgaaacattt ttttgccaat aatggcgata
    12901 agttgccgca tcgtcatgtc tttaacggca tgggttacga tggcgataat atttcaccgc
    12961 atctggcgtg ggatgatgtt cctgcgggaa cgaaaagttt tgttgtcacc tgctacgacc
    13021 cggatgcgcc aaccggctcc ggctggtggc actgggtagt tgttaattta cccgctgata
    13081 cccgcgtatt accgcaaggg tttggctctg gtctggtagc aatgccagac ggcgttttgc
    13141 agacgcgtac cgattttggt aaaaccggat acgatggcgc agcgccgccg aaaggcgaaa
    13201 cccatcgcta catttttacc gttcacgcgc tggatgtaga gcgtattgat gtcgatgaag
    13261 gaggcagcgg cgcgatggtc gggtttaacg ttcatttcca ctctctggca agcgcatcga
    13321 ttaccgcgat gtttagttaa tcactctgcc agatggcgca atgccatctg gtatcactta
    13381 aaggtattaa aaacaacttt ttgtcttttt accttcccgt ttcgctcaag ttagtataaa
    13441 aaagctgaac gagaaacata aaacgctgta aatatcaata tgttatgaaa gatttagtct
    13501 aaataataga ctgcataata ctacaaaaca caacatatcc agtcactatg aatcaactac
    13561 ttagatagta ttagtgacct gaggcagagc attagcgcaa ggtgattttt tgtcctcttg
    13621 cgctaatttt ttgtcaacgg actgggttag ttgaatttac aaccatgcta agtatctcga
    13681 taagcgcaga gaaatgatgc agtggtgggc ggattggctt gatgagaagg ttaggtaggc
    13741 aaatccatgc ctgagcagag ccttgccctc tacctgagcc catgctcatt ctttggcctc
    13801 aacgcgagca tgacctcact ggctggaccc aaaatggact atgacagaaa gatctgatat
    13861 ttttcctcgc aaaaacttag aggctttatt ttaatgcata agagcaactg ctttgacacg
    13921 gcaaggcttg tggcagctat gatggttctg gtcagtcacc actacgcgct ctctgggcag
    13981 ccggagccgt acctgttcgg ctttgaaagt gctggcggca ttgcagttat catattcttt
    14041 tcaatatcag gatatctgat ttcaaaatca gctatcagga gtgattcctt tattgatttc
    14101 atggctaaaa gagcgagaag aatattcccg gcgctggtcc cttgctctat actgacatat
    14161 tttttatttg gatggatact taacgacttc agcgcagaat attttagcca tgacattgtc
    14221 aggaagacca taagctctat ttttatgagt caggctccag atgctgacat tacatcacat
    14281 ttaattcatg ccggaattaa cggcagtttg tggaccctac cgcttgagtt cctgtgttat
    14341 ataataacgg gagtagcagt tgccctacta aagaacggaa aagcattcat agttatactg
    14401 ttggtttttg tttctctttc attaattgga agtgtgtcag aaaacaggga tgtaatgttc
    14461 tctattccgt tatggctgta tcctttgcgt ggactggcgt tcttttttgg tgccactatg
    14521 gcaatgtatg agaagtcatg gaatgtaagt aatgttaaaa ttactgtagt ttctttgctt
    14581 gctatgtacg cctacgctag ttatggcaaa ggcattgatt atactatgac ttgctatatc
    14641 ctggtttcat tctcgacaat agccatatgc acatccgttg gcgacccact ggttaagggc
    14701 aggtttgatt actcgtatgg tgtgtacatt tatgcattcc cggttcagca ggttgttata
    14761 aacacattgc acatgggttt ttacccgtca atgttgttaa gtgccgtgac tgttcttttc
    14821 ttgtcccatc tttcatggaa tctggttgaa aaaagattcc tgacacgaag ttcgcctaaa
    14881 ttatccctgg attgaacaat aaaaggggcc atatggcccc taaaataatc agatggccag
    14941 atactcaacc attgaaggca gcgcgactcc tactgggctt gggcgagtaa cattaattgt
    15001 caccacggaa acactaacct tgtcaccact tgatgcaggg tcaccaaaaa ggcttcttac
    15061 tctgtatgtc aggttccccg ggttcggtat agctgcagat ccgcttgtgg cacctaccgc
    15121 aactgggaca ttaatgatcc catttacaga cggtacgcct gaacagccac gatacgtatt
    15181 gtatctgcct tccggtttct gcacctcggt aatgccagcc aaataacagt cagtaactga
    15241 cacgccaccg ctacgaagat agcgtagagg cttattgaat atgcacttat cgaaagaaac
    15301 acattcattg atgtcgacaa tatttacagc ccattcatca ccagttaacg tacaaccact
    15361 tacaaagacg aagatcgccg actgttctat cgctatactt tgattgtttg cagccgtgtt
    15421 tttgtttgca gtgttgatat gaacaccact gacaatttta tgtgactgcg ttgaggtcgg
    15481 cgttctaaaa tatatccccg taaattgttc agtgccatcg gcatctgctg ctatccatga
    15541 gtttgagaaa gaaaatcctc cgtttacatt ctctataacc acgcctgttg aaccgcaata
    15601 atctaaatcc agttgttcca tatgcaaatc aaggcaatca tgaacataga cggcattttt
    15661 aaaaccaatg cacatggtct cactgtctaa aattattgct tctgacctaa ctgcctctga
    15721 cgaccacgca tacgtttgag gctgaatcct gatgccaaag ctttcatcag cagaataatt
    15781 tcctctgctg aaatggcagg agctgacact aacacttacg cactggccta aaaagtgaag
    15841 accgactcta caggttccag cctgacatgc tataaatttt gagtgccaga gcccttgcat
    15901 taaataagcg gtttcaactt cattcaccat tacattatca aatacccagc cataaacctg
    15961 tctgatgaca gagtcttctg ctgtaatgtt tgtgtcaata ccattgcagg tgttattacc
    16021 agttactcgt atatttctca gataacggcc gatagtgtct gaatcataa
//
LOCUS       DAJTNM010000086        15914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTNM010000086 DAJTNM010000000
VERSION     DAJTNM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.086"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..396)
                     /locus_tag="OO172_002827"
     CDS             complement(<1..396)
                     /locus_tag="OO172_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5512949.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTED"
     gene            complement(393..3077)
                     /locus_tag="OO172_002828"
     CDS             complement(393..3077)
                     /locus_tag="OO172_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5512950.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            complement(3169..3669)
                     /locus_tag="OO172_002829"
     CDS             complement(3169..3669)
                     /locus_tag="OO172_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5512951.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(3699..4415)
                     /locus_tag="OO172_002830"
     CDS             complement(3699..4415)
                     /locus_tag="OO172_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5512952.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            4711..5487
                     /gene="exuR"
                     /locus_tag="OO172_002831"
     CDS             4711..5487
                     /gene="exuR"
                     /locus_tag="OO172_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5512953.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            5832..6494
                     /locus_tag="OO172_002832"
     CDS             5832..6494
                     /locus_tag="OO172_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5512954.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            6498..6881
                     /gene="mzrA"
                     /locus_tag="OO172_002833"
     CDS             6498..6881
                     /gene="mzrA"
                     /locus_tag="OO172_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5512955.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            7028..7382
                     /locus_tag="OO172_002834"
                     /pseudo
     CDS             7028..7382
                     /locus_tag="OO172_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            7420..7725
                     /locus_tag="OO172_002835"
     CDS             7420..7725
                     /locus_tag="OO172_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5512956.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            7728..8132
                     /locus_tag="OO172_002836"
     CDS             7728..8132
                     /locus_tag="OO172_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5512957.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            8122..8421
                     /locus_tag="OO172_002837"
     CDS             8122..8421
                     /locus_tag="OO172_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5512958.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            8607..8999
                     /locus_tag="OO172_002838"
     CDS             8607..8999
                     /locus_tag="OO172_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5512959.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTTLFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            9069..10055
                     /gene="yqjG"
                     /locus_tag="OO172_002839"
     CDS             9069..10055
                     /gene="yqjG"
                     /locus_tag="OO172_002839"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5512960.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            10348..10716
                     /locus_tag="OO172_002840"
     CDS             10348..10716
                     /locus_tag="OO172_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5512961.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(10923..11819)
                     /gene="yhaJ"
                     /locus_tag="OO172_002841"
     CDS             complement(10923..11819)
                     /gene="yhaJ"
                     /locus_tag="OO172_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5512962.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            11924..12625
                     /locus_tag="OO172_002842"
     CDS             11924..12625
                     /locus_tag="OO172_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5512963.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12648..12812
                     /locus_tag="OO172_002843"
     CDS             12648..12812
                     /locus_tag="OO172_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512964.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(13024..14316)
                     /locus_tag="OO172_002844"
     CDS             complement(13024..14316)
                     /locus_tag="OO172_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5512965.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14344..15675)
                     /locus_tag="OO172_002845"
     CDS             complement(14344..15675)
                     /locus_tag="OO172_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5512966.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTCWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT         4146 a         4045 c         3682 g         4041 t
ORIGIN      
        1 atcttctgta catgaagatc tattagaatc cttaagattc gtctgaagcc ctttgccccc
       61 cttgcatcct agcggcgctt catcgctact tacaatggct gttttaaatc ctttgagttt
      121 taccggccaa cgcatcccac tgcgttgttc agttaaatag atttgtactg tcgatatatt
      181 tccattagct gtacaggttt gttggaggtc aggatacgaa cattcatacc acatatatcc
      241 attatttcct gccgacgctt catccttcca gaagtaataa tccgctctac cagatgccaa
      301 atcatacgta atttcactgg tcacgtcagg agctgcctga acgccagtga gcgtgccaaa
      361 caagcccaat atcgccgcaa tcaatcgatt tctcatggtg caacctccct ttgcgccgtc
      421 tgaacgtttc cacgtaattt aggctgtttc aactgtgcct gaacctgaat cgactccggt
      481 aattgtgaat aagtcaccac actacaagta acatctccgc tgtaacgaat gctggcgcgt
      541 ttctttacaa cactcagtgg acacttaagg aattgctgcc cggaaagcag atagagctct
      601 ttttcgctgc cgttaatttc aagtgtaaat ccgccatctt cagctaccag taacatggcg
      661 ctgttcagcc ccacaatcgg gaattttttc accgctgccg gcagtaataa acgaccgata
      721 tagttgtagt taaagctggc agacacttcg cgatagcgca gcttgccagg caacaacatt
      781 aattcacgat tccctgcacc gttaatgatg tttgttgtcc cctgctgatt gccctggcta
      841 ctgtcgttaa cctcaacaaa accagactga aatccaggat aagggaacaa cacactgtca
      901 ctttgaccaa gctgcaaagt tcggctgcct gccacccgcg cttcgagttg cagatcctga
      961 ctctcttcat cctgttcaga gaagcctttc acatccacca gtaccgcacc cagcaaatcg
     1021 tctgtaccac tggctcccaa attaacgcca tagcgactga cagccagcgt agagctataa
     1081 gtgcccgtaa aggccgaaag atgatcatga ttcttacggt cataactatc agatacggta
     1141 gcgtagacat ttccgtattg cccatcccaa cgcccgttaa ccgcgctgtt tatggtatcg
     1201 gtgttcaggc ctccgacggt tacgccaagt tctttatggc tgaatgaatc gttataaaag
     1261 gtatgcgata actgccatga cgtttgatcg ccatcctgtt cgctataacg ataatccgta
     1321 gacacattgg ttgaatggct gttgttattg ctatccatcg ttggcgtgtc agataacgaa
     1381 aacgataagt acaagccgtt gtcgtcgtta tcgccaccgt tgtcattacg atggaaaaca
     1441 ccgactgacg tactcaaaat ccagtcacgc atcacaatag cgcgggaagc gctaagttgt
     1501 acagtttcag agatactttg cgctgttgtc tggcgaagat agttattttc aaagctctgc
     1561 tgtttatccc aacgcaaact atcatcagtg ttattttgtg tgcaggtata acccagcatc
     1621 atattccagc cgacaaaatt cagtgaaact gtcgcggaaa tactttcata acagcttaag
     1681 gcgttatagc tctctctgct gtcggttgta caagcatcgc cgtcagagtt ggtccgataa
     1741 aagcccaacg tcggccatcc cggattactc cagttggcct gttgcatatc acctttgccg
     1801 ccgtcatcgt tacggaacac gctggcgctg attgcaagat tccccacccc gcctaaatct
     1861 gccgtccagt tattacctaa ctcgaaagca ctcacatcat cggcattcgc cagccctgcg
     1921 tagagctcat attgcggatg taatggcagg cgtactccta gctgataagc tgaactttca
     1981 tcatcagaaa cctgtgatgt agttttacct gcctgtaaga accattgcgc atttccgtca
     2041 gtcagaccgc cggttttggt aaacggcaca agctcggtgc gggtgagttg gttattttcg
     2101 tagactttta atgccacgct gtagctgcct ggcggaaagg aactggtatc aataaattgc
     2161 gaaccactat tgagataaaa cgatcccaaa agttgctcat tacgataagc gtcaacacga
     2221 gaattgcgcg aaagcagaac cattactggg gttccttgct ggctttgcgc ctggtttaaa
     2281 tagctcagtg tcgacccgat acgcatccca tcgattgcac cgagtggcag aaagttaaag
     2341 gtaaagttcc cgccttgtgc attaaatagt gtgcggttgt ccatgcgacc cgcctgcaca
     2401 taataacgac gtaaaaaatc ataacgataa tagagatcgc tgacgtcagt attactgtca
     2461 ctgacatcat ctgcatcata accgttgaaa ttccagtgtg caccaatata gctattttct
     2521 gttattccca gcgcaccgtt tccctgaata gacaacgatt ggtaatcatc ctgtgccaga
     2581 acattgatgt cttgctgatg tataaaagcg ttaacagtat ttttgtctgg atttaaatat
     2641 aatgacttcg catcgaaagc tgaattccat tgcggattaa taaacaacgt tgccgaactt
     2701 tcttcatcat tatagattat ttccagcgta tcggtattca gaaaaccaca ccctgagtca
     2761 gtacgtacat aaccgcaagc caattcaccg tggcgagcta gcggctgcga taatttttct
     2821 ttcactatat gcgcgatttt ctgatcgtct acatccagtt ccagcttgtt atatagactt
     2881 gctggatcaa gaaatgttac ggtatcaaga ttaaccattg cataatattt accgagactt
     2941 ttccctgaaa aataaacctc gatatactcc tgctgtccct gagcaataac ctcaaaccct
     3001 gcgggaattt tggttaatgt cgcctctgcc gggctgagac tcgccaggat caaaagagcc
     3061 agtaattttt tatccatggc aatcctgaaa tccataacaa atactaaaac agttaaaata
     3121 agggagggct acccctccct ttgttacgtg ttaattctgt tatccggatt aaatatcctg
     3181 agacagataa atgcttacta caccacggta gatgcctgtt tccagaacac ctttggtggt
     3241 ttgagcaaac atcaggtttt tagccagaga accgttagtt aacgcatcgg aagcaaagat
     3301 tttggttgcg gtaaaagtgg ttgccgcatc agctttaatt tcttcaccac cccaggttac
     3361 tgtcagcgga acaatcttgc tagcatccag gctttgaacc agttgcgctg gggacactaa
     3421 ctgcattttt acatctttgg taacgtcgtt agaccagatt ttggtcatca actggtaaga
     3481 ttgcagaccc tgccccggca gatattgcat ttctactgct ttcggcaacg cggtgttatc
     3541 ggtctgtgtc atatccagag cggcatcaac gttggccgtt acagtaatat ccttttgtac
     3601 tgccaatgcg gagcctgcaa cagaaaacat cgctgcgacc agaagagatt ttgcaaatac
     3661 ctttttcatt actattcctt cgtttattac aatgataatt aatggaccgc cagttcaaac
     3721 tcgccattat ctttggtgat ccagttatta tatttgacaa cgattttgtc gtagccagat
     3781 atgctttcaa gatttctttc ctgaccaggg aatatgtttt tatttacggt ctgtatctta
     3841 cagttttctt tgctattatt tttacaataa gcgatcgtta aaagctttaa gcgttgattc
     3901 cctgcattaa ttaatttttg ggcagaattt acttctaact tagctatagg ttgctgaggt
     3961 ggaacactaa caacgattcc ccaaattaag ttaactgtta actgcataga gagatctttt
     4021 ttatctgtgg caacgtcatc aatactggga accgcttcaa atttaacgcg ataatttttc
     4081 tctttttgct ctggttccat cgcaacaaaa cgtacggttt tgctactacc ggctggtaaa
     4141 gcgaatttag gtggcataac aaccagatga tttgcatcac cggatttaat ctcaacttca
     4201 ttttcctgag gcgtcgatgg attatcgata cggaatactg tcgccttgat gtactgaact
     4261 tcattacttt tagatattac gcgaacgttg ccttcaccct gactattaat ggaaacaggc
     4321 ataggataaa cagtcatatt tgactgtgcc aaaaaaggag cgaacacgaa taaaagcggc
     4381 gtaagccgta ataagagatt aattttcgcc accatataat tttgatctta caatatcgtt
     4441 tcaaaaaagt tggtgtatta gagatgtaga tttattgtaa gtcaatataa aaaagcattt
     4501 ttagttaaca ttaaatttaa aggctgggaa ttaacttact gaaatttagt agctttcaca
     4561 atgacgatac ttatttttta ggaatttata aatattcaca atattaattt aatacattaa
     4621 ttcaatcatc aaatttatat atgaccaatg gcatacccat gaataaagtg gtatgataac
     4681 ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg ccgtttgtat
     4741 caacaacttg ctgctgacct gaaagagcgc attgaacagg gcgtctatct ggtgggtgat
     4801 aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac ggtagttcgt
     4861 gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg ttcaggtatt
     4921 catgtggttt ccaaccagcc gcgtcatcag caggcggctg acaataatat ggaattcgcc
     4981 aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa tattgccgag
     5041 ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat tcaggaacag
     5101 gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca cattcaggta
     5161 gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg gacccagcgt
     5221 agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac cgttgataac
     5281 tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc tcatgctgct
     5341 aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa cgaaaccagc
     5401 gacgatttcg agttcaatgc cgaccgctat ctgttcgccg aaaacccggt agtgcatctc
     5461 gatacggcca ccagcggcag caaatgaaat tcctgtccga caggcgctgc aatgcgcctg
     5521 tttgtctcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc tctcgccacc
     5581 ctcccctgca atcagcaaaa tcagactcca cggacatgta attttgataa cgaacaacgt
     5641 tgacctttgt tacaattaga ttcaatttga atttatgctt ttgaatgctt tcttatctca
     5701 cgttttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga acataccaaa
     5761 acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa ccgattcacc
     5821 aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg caggattttg
     5881 aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt gtaattttgt
     5941 tccttgaaaa tggcttgctt ccggcggcct ttttaccggg cgacagttta ctggtattgg
     6001 tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg ctgctgaccg
     6061 ttgccgccag cctcggttgc tgggtcagct atattcaggg gcgatggctg ggcaataccc
     6121 gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc gcacaccatc
     6181 tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgca tttgtcagaa
     6241 cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt cagttcttca
     6301 actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac atgctcggca
     6361 aaacgccggt atttttaaag tacgaggacc agctgatgtc atgcctgatg ctgctcccgg
     6421 tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa aagaaatatg
     6481 gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta gcctggtccg
     6541 gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt cgccagcaag
     6601 agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac ggtttttcga
     6661 tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc aaaaacgaca
     6721 ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg gtcctcgaca
     6781 gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag gctatgcagt
     6841 ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt atccgaaaca
     6901 tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg gtggttaaca
     6961 ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca caatggaagg
     7021 ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta gtgccggtag
     7081 ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga tcagctatgc
     7141 cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga gtgaagtccg
     7201 ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg caaagcagaa
     7261 agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag gcgatgccga
     7321 tagaacggaa actggcagaa gcgcaggaag agctgaaaaa gctggaagcg cgcgactact
     7381 aactcacaat agtcactact tactcacctg gagaaaacta tgtcgaaaga acacactacg
     7441 gaacatctgc gtgctgagtt gaaatccctt tccgatacgc tggaagaggt gcttagctca
     7501 tctggcgaga agtcgaaaga agagttgagt aagattcgta gcaaagcgga gcaggcactg
     7561 aaacagagcc gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca aacccgtgtc
     7621 gcggcggcgc gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt gggaattggc
     7681 gctgcaatcg gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg gcggacactc
     7741 atcacgcgca agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca
     7801 tggttgaaat ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag
     7861 ctaatctctt tcaactttta ctgatgctgg gcctgacgat gcttttcgct gcatttggtc
     7921 ttatgagcct gatggtgcta attatttggg cggttgatcc gcaatatcgc ctgaatgcga
     7981 tgattgccac caccgtggtg ttgctgctac tggcactgat tggcggtatc tggacgttac
     8041 gtaaatcgcg taaatccacc ttgctgcgcc atacacgcca tgagctggca aacgatcggc
     8101 aactgctcga ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg taaggcgcaa
     8161 ctgcttagcc agatccagca acaacggctg gatctttccg ccagtcgtcg tgaatggctg
     8221 gaggcaacag gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg ctcctgggcg
     8281 ctggttggca gtagcgtgat ggcgatctgg acgattcgcc atcctaatat gctggtccgc
     8341 tgggccagac gcggttttgg cgtatggagc gcctggcgtc tggttaaaac gaccctcaag
     8401 cagcaacagc ttcgcggtta acttccctct ggccggagca attccggcct tatccctcaa
     8461 attttttgaa tatttttgac agttttcctt gctaacaatc atcattcacc acgtttatga
     8521 ttctctccat cgacagcaac gacgctaata ccgcgccctt gcacaaaaaa acaatcagca
     8581 gcctgagtgg cgcagtggag agtatgatga aaaaattaga agatgttggt gtactggtag
     8641 cgcacatttt aatgccgatt ctgtttatta ccgcaggctg gggaaaaatc accgcttact
     8701 ctggtaccca acaatatatg gaagcaatgg gtgtcccggg ttttatgctg ccgctggtga
     8761 ttctgcttga gttcggtggt ggtctggcaa tcctattcgg tttcctgact cgcaccacaa
     8821 ccctgtttac tgcgggcttt acgctgctga cggcattttt atttcacagc aactttgctg
     8881 aaggcgtcaa ctcgctgatg ttcatgaaaa acctgacaat ttctggcgga ttcctgctgc
     8941 tggcaattac cggtccgggc gcgtatagca tcgaccgcct gctgaataaa aagtggtaag
     9001 cacgcgctat acttaacgat aaaaaacgag gaggaagctc ctcgtttttg ctattggagg
     9061 agagaaaaat gggtcaactg attgacggcg tctggcatga cacctggtac gataccaaat
     9121 ctaccggcgg taaatttcaa cgttcagctt ccgcatttcg taactggctc actgccgatg
     9181 gcgcacctgg ccccactggc acaggcggtt ttatcgcaga gaaagatcgt tatcatctct
     9241 atgtttcact cgcctgcccg tgggcgcacc gcacgctgat catgcgcaaa ctcaaaggac
     9301 tggaaccgtt tatttccgtt tccgtagtga acccgctgat gctggaaaac ggctggacct
     9361 ttgatgacag ttttccggga gcaaccggag atacgctcta tcaacatgaa tttctgtatc
     9421 agctttatct ccacgccgat ccacactaca gcggacgagt tactgttccc gtgctgtggg
     9481 acaaaaagaa ccacaccatc gtcagcaacg aatcagcaga aatcatccgc atgttcaata
     9541 ccgcgtttga tgcgctgggc gcaaaagcgg gtgattacta cccaccagcc ctgcaaacga
     9601 aaattgacga acttaacggc tggatttatg acaccgttaa caacggcgtg tataaagctg
     9661 gttttgccac cagccagcaa gcctacgacg aggcggtggc gaaagtgttt gaatcgctgg
     9721 cgcgactgga gcagatttta ggccagcatc gttacctgac cggcaaccag ctaaccgaag
     9781 ccgatattcg cctgtggact acgctggtgc gttttgatcc agtgtatgtg acccacttca
     9841 agtgtgataa gcaccgcatc agcgattacc tgaatctgta tggcttcctg cgcgatatct
     9901 accagatgcc ggggatcgcc gaaactgtca atttcgatca catccgtaat cattacttcc
     9961 gcagccataa gaccatcaac cctacgggga ttatttcaat tggtccgtgg caggatctcg
    10021 atgaaccgca tggacgagat gttcgcttcg gttaaaaaaa ggcaccttac gaggtgcctc
    10081 tgataattag ctaagtgcat gttgttcatg tcggatgcgg cgtgaacgcc ttatcaaacc
    10141 tacaaagcgt ttatattcaa tatgttacaa tgaccgtgta ggccagataa gcgtagcgca
    10201 tctggcgatt ttgcgcttgc catcagtctt agcgcgcctt cacggcactt tattaattcc
    10261 ttgaacgaat atttactgcc atttcattca cgtctattct taatttgctg ctttaaaaac
    10321 aagtgattga gcaaaattga ggcaaaaatg gactggtatc tgaaagtact aaaaaattat
    10381 gtcggtttcc ggggtcgtgc gcggcgcaaa gagtactgga tgtttattct ggtcaacatc
    10441 atctttacgt tcgtgctggg gctgctggat aaaatgttag gctggcaacg cgctggtggc
    10501 gaaggcatcc tgacgacaat ctacggtatt ctggtgtttt taccgtggtg ggcggttcag
    10561 ttccgccgcc tgcacgacac cgaccgctcg gcgtggtggg cactgctatt cttaatcccg
    10621 tttatcggct ggcttattat catcgtcttt aactgccagg cgggtacgcc aggcgaaaac
    10681 cgctttggac cagatccaaa actggaacaa gaataatgat taagcacctt gtaaaaggtg
    10741 cttcaaattg ttgacaaagt gcgctttgtt tatgccggat gcggcgtaaa cgccttatcc
    10801 agcctacaaa ttgccaaaat tcaatatatt gcagtgactg cgtaggcctg ataagcgtag
    10861 cgcatcaggc agttttgcgt ttgtcagcag tcagaagcac cttgtgaaag gtgcttaata
    10921 gattattttc cagcgaacaa ctttggaatt tcccgcagac accaggattt cgcttccccc
    10981 atactgtcac gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg
    11041 acacgcaacc gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg
    11101 ccaagccccg ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc
    11161 tgtttgtcca gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca
    11221 attccacgat atttcacacg cgtcacttca gataacggtt ccggctcctg atgaatcggg
    11281 tgatcaggcg cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc
    11341 gacgaggaac gaaaatgcat atccggcgcg ataacgatat ccgcccgccc ctgctccagc
    11401 cgttcccacg cccccgccag cacttctgtg atgattgcca gttgggtatt ggcttttgcc
    11461 gccagtttgt cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg
    11521 gtgagatgcg tttcccaacc gcgcgcgaga gcttccgcat cagtagtcag tttatctgcg
    11581 gcttccagca aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg
    11641 cgatggcccg agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatgatg
    11701 tagctaagtg cggaaggcac gcgtcccagc tcatccgctg ccgccgcaaa actgccccgg
    11761 cgatcgatcg catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt
    11821 tcgttctcat tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt
    11881 ccagcccctt accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac
    11941 tgccaggcag tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt
    12001 tggacactac ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc ttaaccagga
    12061 agtgctggcc ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa
    12121 tgtgattctg gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag
    12181 cgccggagag gcgttgctgc tctctaccca gccgggtgtc agctatagcg aacacaatct
    12241 cagcaaagac aaaccgttaa cgcgaatgca gctttggctg gatacctgcc cgcagcgaga
    12301 gaatccgctg attcaaaagc tggcgcttaa tatggacaag cagcaattaa tcgcctcgcc
    12361 agagggtgcg atgggaagcc tgcaattacg ccaacaagtg tggctgcacc atatcgtgct
    12421 cgacaaaggc gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat
    12481 tcacgggaaa tttcatgcgc ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga
    12541 tggggcgttt attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc
    12601 tttgctgata gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat
    12661 cgaccaaaaa gcgccagcta gcgaagcccg tggtggcgaa agaacctgct cgcaccgcca
    12721 aaaattttgg ctatgaagag atgttgagcg agctggaagc tatcgtcgcg gatgctgaaa
    12781 cgcgtttagc cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc ggcgtaaacg
    12841 ccttatccgg cctacaaaac acaacaacat caacaaatta aacaccccgt aggccggata
    12901 agatgcgcca gcatcgcatc cggcatcagg tacaaaacac aacaacatca acaagttgag
    12961 caccccgtag gccggataag gtgcgccagc atcgcatccg gcacaatact caaaacctgg
    13021 cgtttatctg gccttgctcg ccataatctc gataatctgc cgatccgttt gctgcatcga
    13081 atggcttgct aacgcacaca ggttggcaat cgactgctca acatcatgcg ccacgatccc
    13141 ttcattgccg gtcacggcgg tatcatccag cgccattaac accgctttcc acgcagccga
    13201 agcactggtc gaaaccttcg caccatcgca aatcatgccg ctgacatcgc cgatcatact
    13261 gctgatcgcc atcgagatag tttcataacg cccatccacc agccatgcca tcccggcggc
    13321 ggcccccatt gctgcggtcg ttgcggcaca aagtgcagac aaacgcggta actggttatg
    13381 gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagtcgtt catcatccgc
    13441 tccgaagtgt tctgctacaa ccaccacagg cattgttgcg gtgatcccct ggttacccga
    13501 gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct
    13561 ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct cgcactgttt
    13621 ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct gcgataacgc
    13681 acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaatggga cttcattgac
    13741 gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggcg actcttgctc
    13801 gccctctgcc acacacgcct gctgggtaaa caccacacca ttgtgcgtct caatatgcac
    13861 aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt tccagacttt
    13921 ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacagaga ctttccccgc
    13981 cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gcgtctttca gcacttccag
    14041 cccggcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca gccccaccat
    14101 tcccgtgccg ggaacggtga tgcccagacc gttcttcatc agatttggcg aaacccaggc
    14161 ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg ccagcgccag
    14221 tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta
    14281 acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa aatttcaggt
    14341 aaatcaagaa aatgccagga acagagaaac acaaagcaac aaaccagtga cgataatcag
    14401 gtacagagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca gccacgccgg
    14461 gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg
    14521 tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga tgccgcgctg
    14581 aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt tcattacgat
    14641 cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca tgacagcaaa
    14701 gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc taataaactg
    14761 tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc
    14821 catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc
    14881 gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt cccgcgagcg
    14941 ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca gggtaaacgg
    15001 cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac cgacgttata
    15061 cagatgccac attcccacca tcgacacgcc cagcgcagcg actaccagca gcttggtcag
    15121 caccatgtcg gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga tcgccaccag
    15181 aatgcaaatc agtaccagac cataaaaggg actgtctgac agcaaccctt ccgtcacgcc
    15241 gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga cgaacatcca
    15301 aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt tacctaaata
    15361 accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg tattaataaa
    15421 caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca aaaataccca
    15481 caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc
    15541 gatactcata atcacccagc aggtgtcagt actgtcgaat ttgatcgctt ctcgccactc
    15601 gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt
    15661 cgatgcaatt tccataattt ctcgctcaat attttgtagg gcttattatt tttttccgag
    15721 ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga gttattagaa taaagaaatg
    15781 atacgcgcga ggtcagagaa aatcttcaca atcaagactt taaaataaca gagatggata
    15841 agaattttct actaaattaa tcgcaagaga atattcattc tctatttgcg gcagatcaca
    15901 aaaaaaaggt gcac
//
LOCUS       DAJTNM010000087        15807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTNM010000087 DAJTNM010000000
VERSION     DAJTNM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.087"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="OO172_002846"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="OO172_002846"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5512967.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="OO172_002847"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="OO172_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5512968.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="OO172_002848"
     CDS             2337..2552
                     /locus_tag="OO172_002848"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5512969.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="OO172_002849"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="OO172_002849"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5512970.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /gene="galP"
                     /locus_tag="OO172_002850"
     CDS             4447..5841
                     /gene="galP"
                     /locus_tag="OO172_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5512971.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWRTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="OO172_002851"
     CDS             5918..6415
                     /locus_tag="OO172_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5512972.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="OO172_002852"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="OO172_002852"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5512973.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="OO172_002853"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="OO172_002853"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5512974.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="OO172_002854"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="OO172_002854"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5512975.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9100..9663
                     /locus_tag="OO172_002855"
     CDS             9100..9663
                     /locus_tag="OO172_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5512976.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="OO172_002856"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="OO172_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5512977.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="OO172_002857"
     CDS             complement(10255..11235)
                     /locus_tag="OO172_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5512978.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="OO172_002858"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="OO172_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5512979.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="OO172_002859"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="OO172_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5512980.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="OO172_002860"
     CDS             12538..12828
                     /locus_tag="OO172_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5512981.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANNHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="OO172_002861"
     CDS             12836..13429
                     /locus_tag="OO172_002861"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5512982.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITHEPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="OO172_002862"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="OO172_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5512983.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15807
                     /locus_tag="OO172_002863"
                     /pseudo
     CDS             14872..15807
                     /locus_tag="OO172_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         3915 a         4094 c         3991 g         3807 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttctg cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttctaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaggc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga atcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaaaaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggtcgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggtctgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg cctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaattga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 acttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac catattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtgga ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtctcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 agccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcactcgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccga ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gcgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgatcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata ccatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgtcatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacggc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa caatcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cacgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgttcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ccatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aagcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtgac gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattacc
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgtt
//
LOCUS       DAJTNM010000088        15789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTNM010000088 DAJTNM010000000
VERSION     DAJTNM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.088"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..378
                     /gene="tuf"
                     /locus_tag="OO172_002864"
     CDS             <1..378
                     /gene="tuf"
                     /locus_tag="OO172_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5512984.1"
                     /translation="RAGENVGILLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILS
                     KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLG"
     gene            561..755
                     /gene="bfd"
                     /locus_tag="OO172_002865"
     CDS             561..755
                     /gene="bfd"
                     /locus_tag="OO172_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5512985.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            828..1304
                     /gene="bfr"
                     /locus_tag="OO172_002866"
     CDS             828..1304
                     /gene="bfr"
                     /locus_tag="OO172_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5512986.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1301..1768)
                     /locus_tag="OO172_002867"
     CDS             complement(1301..1768)
                     /locus_tag="OO172_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5512987.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            2147..2458
                     /gene="rpsJ"
                     /locus_tag="OO172_002868"
     CDS             2147..2458
                     /gene="rpsJ"
                     /locus_tag="OO172_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5512988.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2491..3120
                     /gene="rplC"
                     /locus_tag="OO172_002869"
     CDS             2491..3120
                     /gene="rplC"
                     /locus_tag="OO172_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5512989.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3131..3736
                     /gene="rplD"
                     /locus_tag="OO172_002870"
     CDS             3131..3736
                     /gene="rplD"
                     /locus_tag="OO172_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5512990.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3733..4035
                     /gene="rplW"
                     /locus_tag="OO172_002871"
     CDS             3733..4035
                     /gene="rplW"
                     /locus_tag="OO172_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5512991.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4053..4874
                     /gene="rplB"
                     /locus_tag="OO172_002872"
     CDS             4053..4874
                     /gene="rplB"
                     /locus_tag="OO172_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5512992.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4891..5169
                     /gene="rpsS"
                     /locus_tag="OO172_002873"
     CDS             4891..5169
                     /gene="rpsS"
                     /locus_tag="OO172_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5512993.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5184..5516
                     /gene="rplV"
                     /locus_tag="OO172_002874"
     CDS             5184..5516
                     /gene="rplV"
                     /locus_tag="OO172_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5512994.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5534..6235
                     /gene="rpsC"
                     /locus_tag="OO172_002875"
     CDS             5534..6235
                     /gene="rpsC"
                     /locus_tag="OO172_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5512995.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6248..6658
                     /gene="rplP"
                     /locus_tag="OO172_002876"
     CDS             6248..6658
                     /gene="rplP"
                     /locus_tag="OO172_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5512996.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6658..6849
                     /gene="rpmC"
                     /locus_tag="OO172_002877"
     CDS             6658..6849
                     /gene="rpmC"
                     /locus_tag="OO172_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5512997.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6849..7103
                     /gene="rpsQ"
                     /locus_tag="OO172_002878"
     CDS             6849..7103
                     /gene="rpsQ"
                     /locus_tag="OO172_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5512998.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7268..7639
                     /gene="rplN"
                     /locus_tag="OO172_002879"
     CDS             7268..7639
                     /gene="rplN"
                     /locus_tag="OO172_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5512999.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7650..7964
                     /gene="rplX"
                     /locus_tag="OO172_002880"
     CDS             7650..7964
                     /gene="rplX"
                     /locus_tag="OO172_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5513000.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7979..8518
                     /gene="rplE"
                     /locus_tag="OO172_002881"
     CDS             7979..8518
                     /gene="rplE"
                     /locus_tag="OO172_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5513001.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8533..8838
                     /gene="rpsN"
                     /locus_tag="OO172_002882"
     CDS             8533..8838
                     /gene="rpsN"
                     /locus_tag="OO172_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5513002.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8872..9264
                     /gene="rpsH"
                     /locus_tag="OO172_002883"
     CDS             8872..9264
                     /gene="rpsH"
                     /locus_tag="OO172_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5513003.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9277..9810
                     /gene="rplF"
                     /locus_tag="OO172_002884"
     CDS             9277..9810
                     /gene="rplF"
                     /locus_tag="OO172_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5513004.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9820..10173
                     /gene="rplR"
                     /locus_tag="OO172_002885"
     CDS             9820..10173
                     /gene="rplR"
                     /locus_tag="OO172_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5513005.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10188..10691
                     /gene="rpsE"
                     /locus_tag="OO172_002886"
     CDS             10188..10691
                     /gene="rpsE"
                     /locus_tag="OO172_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5513006.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10695..10874
                     /gene="rpmD"
                     /locus_tag="OO172_002887"
     CDS             10695..10874
                     /gene="rpmD"
                     /locus_tag="OO172_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5513007.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10878..11312
                     /gene="rplO"
                     /locus_tag="OO172_002888"
     CDS             10878..11312
                     /gene="rplO"
                     /locus_tag="OO172_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5513008.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11320..12651
                     /gene="secY"
                     /locus_tag="OO172_002889"
     CDS             11320..12651
                     /gene="secY"
                     /locus_tag="OO172_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5513009.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12683..12799
                     /gene="rpmJ"
                     /locus_tag="OO172_002890"
     CDS             12683..12799
                     /gene="rpmJ"
                     /locus_tag="OO172_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5513010.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12946..13302
                     /gene="rpsM"
                     /locus_tag="OO172_002891"
     CDS             12946..13302
                     /gene="rpsM"
                     /locus_tag="OO172_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5513011.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13319..13708
                     /gene="rpsK"
                     /locus_tag="OO172_002892"
     CDS             13319..13708
                     /gene="rpsK"
                     /locus_tag="OO172_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5513012.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13742..14362
                     /gene="rpsD"
                     /locus_tag="OO172_002893"
     CDS             13742..14362
                     /gene="rpsD"
                     /locus_tag="OO172_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5513013.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14388..15377
                     /gene="rpoA"
                     /locus_tag="OO172_002894"
     CDS             14388..15377
                     /gene="rpoA"
                     /locus_tag="OO172_002894"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5513014.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15418..>15789
                     /gene="rplQ"
                     /locus_tag="OO172_002895"
     CDS             15418..>15789
                     /gene="rplQ"
                     /locus_tag="OO172_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5513015.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEA"
BASE COUNT         4061 a         3714 c         4217 g         3797 t
ORIGIN      
        1 cgtgctggtg agaacgtagg tattctgctg cgtggtatca aacgtgaaga aatcgaacgt
       61 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      121 tacattctgt ccaaagatga aggcggccgt catactccgt tcttcaaagg ctaccgtccg
      181 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      241 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc taatccaccc gatcgcgatg
      301 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgtt
      361 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
      421 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      481 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      541 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      601 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      661 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      721 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      781 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      841 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      901 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      961 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
     1021 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
     1081 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
     1141 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
     1201 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
     1261 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
     1321 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1381 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1441 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ccaggcgcca agggcggcga
     1501 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1561 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1621 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1681 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1741 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1801 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1861 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1921 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1981 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     2041 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     2101 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     2161 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     2221 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     2281 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     2341 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2401 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2461 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2521 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2581 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2641 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2701 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2761 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2821 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2881 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2941 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     3001 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     3061 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     3121 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     3181 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     3241 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     3301 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3361 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3421 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3481 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3541 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3601 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3661 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3721 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3781 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3841 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3901 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3961 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     4021 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     4081 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     4141 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     4201 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     4261 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     4321 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4381 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4441 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4501 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4561 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4621 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4681 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac tgcgatgaac ccggtagacc
     4741 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4801 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4861 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4921 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4981 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     5041 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     5101 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     5161 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     5221 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     5281 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     5341 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5401 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5461 cagatcgtat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5521 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5581 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5641 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5701 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5761 tatcggtaag aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5821 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5881 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5941 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     6001 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     6061 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     6121 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     6181 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     6241 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     6301 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6361 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6421 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6481 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6541 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6601 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6661 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6721 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6781 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6841 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6901 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6961 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     7021 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     7081 ttgtagagaa agcggttctg taatacagta cactctctcg atatgaataa acggctcaga
     7141 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     7201 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     7261 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     7321 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7381 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7441 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7501 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7561 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7621 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7681 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7741 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7801 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7861 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7921 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7981 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     8041 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     8101 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     8161 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     8221 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     8281 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     8341 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8401 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8461 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8521 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8581 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8641 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8701 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8761 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8821 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8881 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8941 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     9001 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     9061 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     9121 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     9181 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     9241 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     9301 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9361 aaaaacggcg agctgacccg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9421 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9481 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9541 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9601 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9661 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9721 cgcgcctacc gtcgtcctga gccttacaaa ggcaagggtg ttcgttacgc cgacgaagtc
     9781 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9841 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9901 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9961 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
    10021 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
    10081 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
    10141 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
    10201 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
    10261 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
    10321 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10381 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10441 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10501 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10561 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10621 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10681 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10741 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10801 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10861 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10921 cgggtaaacg cctggggcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10981 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    11041 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    11101 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    11161 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    11221 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    11281 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    11341 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11401 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11461 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11521 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11581 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11641 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11701 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11761 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11821 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11881 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11941 gagcaagcgc gtcaaggtga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    12001 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    12061 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    12121 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    12181 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    12241 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    12301 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12361 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12421 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12481 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12541 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12601 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12661 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12721 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12781 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12841 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12901 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12961 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    13021 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    13081 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    13141 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    13201 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    13261 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    13321 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13381 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13441 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13501 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13561 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13621 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13681 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13741 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13801 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13861 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13921 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13981 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    14041 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    14101 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    14161 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    14221 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    14281 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    14341 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14401 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14461 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14521 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14581 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14641 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14701 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14761 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14821 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14881 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14941 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    15001 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    15061 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    15121 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    15181 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    15241 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    15301 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15361 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15421 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15481 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15541 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15601 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15661 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15721 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15781 gcagaagct
//
LOCUS       DAJTNM010000089        15726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTNM010000089 DAJTNM010000000
VERSION     DAJTNM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.089"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(6..1001)
                     /gene="yjfF"
                     /locus_tag="OO172_002896"
     CDS             complement(6..1001)
                     /gene="yjfF"
                     /locus_tag="OO172_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR5513016.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(988..2010)
                     /locus_tag="OO172_002897"
     CDS             complement(988..2010)
                     /locus_tag="OO172_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5513017.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNLVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2024..3526)
                     /locus_tag="OO172_002898"
     CDS             complement(2024..3526)
                     /locus_tag="OO172_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5513018.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDMREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLNTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3666..4623)
                     /gene="ytfQ"
                     /locus_tag="OO172_002899"
                     /pseudo
     CDS             complement(3666..4623)
                     /gene="ytfQ"
                     /locus_tag="OO172_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4933..5463
                     /gene="ppa"
                     /locus_tag="OO172_002900"
     CDS             4933..5463
                     /gene="ppa"
                     /locus_tag="OO172_002900"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5513019.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5843..6184)
                     /locus_tag="OO172_002901"
     CDS             complement(5843..6184)
                     /locus_tag="OO172_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5513020.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6187..9966)
                     /gene="tamB"
                     /locus_tag="OO172_002902"
     CDS             complement(6187..9966)
                     /gene="tamB"
                     /locus_tag="OO172_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5513021.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9963..11696)
                     /gene="tamA"
                     /locus_tag="OO172_002903"
     CDS             complement(9963..11696)
                     /gene="tamA"
                     /locus_tag="OO172_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5513022.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11902..12540
                     /gene="msrA"
                     /locus_tag="OO172_002904"
     CDS             11902..12540
                     /gene="msrA"
                     /locus_tag="OO172_002904"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5513023.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12863..14206
                     /locus_tag="OO172_002905"
     CDS             12863..14206
                     /locus_tag="OO172_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5513024.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14268..14474)
                     /locus_tag="OO172_002906"
     CDS             complement(14268..14474)
                     /locus_tag="OO172_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5513025.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14799..15353
                     /locus_tag="OO172_002907"
     CDS             14799..15353
                     /locus_tag="OO172_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5513026.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPRSQFIVDSNGVARGAWQLDDESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15416..>15726)
                     /locus_tag="OO172_002908"
     CDS             complement(15416..>15726)
                     /locus_tag="OO172_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513027.1"
                     /translation="AIVIESLGFALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAG
                     CQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQLALPVLVRCKNSET
                     "
BASE COUNT         3731 a         4321 c         3889 g         3785 t
ORIGIN      
        1 catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg attctcccac
       61 agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc gatggcgatt
      121 ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat cagtccctga
      181 attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa caacgtaccg
      241 ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag tgcatatccg
      301 gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt ggagagcata
      361 taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga cgtcgcgttg
      421 ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa aataccgata
      481 accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc ggggattttc
      541 cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga ctcttccgaa
      601 acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat aaatgccggg
      661 atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc catcaccagc
      721 actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt tgccagaaac
      781 acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga gaggatcaca
      841 aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat attgcagatc
      901 acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc cagcacaaag
      961 acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta ctcctttaat
     1021 cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca ccgctttcac
     1081 caccaggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca tcccctgaat
     1141 aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca gcgatccgcc
     1201 gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt tgttggcatc
     1261 ggcaccgcaa atatccgccg ccacgataat gcccgcaatc gccgcacata gcccgctcaa
     1321 cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg ccgcccgaat
     1381 gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca acagccagaa
     1441 caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca gcgatccgct
     1501 gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg tgatcagttg
     1561 cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct gaattttgag
     1621 gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca gggcgcttaa
     1681 caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg ctccggcgat
     1741 agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca gcgtcatacc
     1801 aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc taccgaacaa
     1861 acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat cgaccagcag
     1921 caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac gcctttttgg
     1981 cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc gcaatggcgt
     2041 tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt ttgcgatcgc
     2101 gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag gagatcacca
     2161 gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg gcgtgggcac
     2221 caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg gtcagtagcc
     2281 aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc ggttgttcag
     2341 ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct tgctgctctt
     2401 tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcaaggatg atattttccc
     2461 gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag aatccgatgc
     2521 ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg atcaacgccg
     2581 tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta cgtccggatc
     2641 ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca aacggtgcta
     2701 tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc aacaatgttc
     2761 gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt tttaccagtt
     2821 cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg cgtaagacgg
     2881 tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag atcaggctga
     2941 cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttct tgggtgttga
     3001 ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata gcccggcaaa
     3061 tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc atgtcgaggg
     3121 agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta cggcgtagaa
     3181 ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac atattgggta
     3241 gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg gcggtatttt
     3301 tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg tatacaccag
     3361 ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc gccatgattt
     3421 cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg aaaaatttac
     3481 ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt tcccccggtg
     3541 taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag gcttcagtgc
     3601 accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat caggcatagc
     3661 aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt atcaggcagg
     3721 taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt tttgtatttc
     3781 tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact ggcgttcgct
     3841 tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc cgtgaggata
     3901 tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat caccatgtcg
     3961 tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt gataaagctc
     4021 tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg gataattttg
     4081 atatttggcg cattcttaat ggcttcggca aagcctttct taacggtcaa tggcgacgct
     4141 ggccccaacg gtgccctgca gctccaccac gttgcatggt ttgccattca cttcttttac
     4201 cagccagtca ccgatcaact taccttcgag gatgttgtcg gcagtaacgg tggtcatata
     4261 gagagatttg tctttcacat cgatggaacg gtcgagcaag aagaccggga tttcggcatc
     4321 tttcgcctct tttaataccg gttcccaacc cgtcgcaacc actggagcga taaagatcgc
     4381 atccacccct tgtgcaacga aggaacgtac cgctttaatc tggttttcct gcttttgctg
     4441 accatcggca attttcaacg taattccgcg tttttcggcc tcacttttcg ccacattggt
     4501 ttctgcggcg cgccagccgg attccgatct gacctgcgaa aatcctacgg ttaatggagc
     4561 ggccaacgcc atagacgaca tggctgccga gactgcagag actacaagta agcgtttcca
     4621 catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc gaaaaaacta
     4681 tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt aaataaaacg
     4741 ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcta cggttatgga
     4801 ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag gaaaatgaag acgaaaacaa
     4861 gcaaagacat tcggcgcgag ttggctataa tacttggcac ttgtttgcca catattttta
     4921 aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc ggaagacatc
     4981 tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat cgacaaagag
     5041 agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc gtgcaactac
     5101 ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact ggtcccgact
     5161 ccgtacccgc tgcagcctgg ttctgtgatc cgttgccgtc cggttggcgt tctgaaaatg
     5221 accgacgaag ccggtgaaga tgcgaaactg gttgcggttc cgcacagcaa gctgagcaaa
     5281 gaatacgatc acattaaaga cgtaaacgat ctgcctgaac tgctgaaagc gcaaatcgct
     5341 cacttcttcg agcactacaa agacctcgaa aaaggcaagt gggtgaaagt tgaaggttgg
     5401 gaaaacgcag aagccgctaa agctgaaatc gttgcttcct tcgagcgcgc aaagaataaa
     5461 taagttcttc tagcgcaata accctgaacg ccgggcttcg gttagtaagg gtttttttat
     5521 gcccgcgata aataaactct ctattccacc atcattattc tcagcggttg caaggcttga
     5581 acggtaagaa caagcaaacc cgaccaccat tttgctgttc atagccactt gctggaagtt
     5641 agccgacctc actcatactc accgttcaaa gcgtgccccc gttttacccc tgcacatttg
     5701 tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg gagagaaaag
     5761 caaaagatgg aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa caacgccacc
     5821 cgaaggaggc gtgcttttat tattacttat ccctgtctaa ccagtcgccg ctttcaatta
     5881 gctttaatcc atcgacgggt cgttgataaa cgtacatcca tgcactccca tacggcgtct
     5941 gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt tcggccagcg
     6001 tggcgttgtc aatacgataa acttcaccgt gtaccgttcc gttccccgga actgcgcctg
     6061 gatagtggcc caggctatac aactggtagt tatcgatact gaaatcgccc agtaactggg
     6121 cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag acaaatattc
     6181 gcattgctaa aactcgaact gatagagcaa atccagtgcc tggtctacac cagacacggc
     6241 ttccagatat agcttaggca tcaggcgata acgtaacgtg agtgttgcta tagagtcaaa
     6301 tatacccacg ccgtatttca cttgcagacc tggcaataca tagccgctga ccactacctg
     6361 ggaggagtcg cctactccca gggtgtcgag cgctaaattg cttacgccaa acgtctcgcc
     6421 gattttaccc acaatctggc cactttgcgc aacccccaga ccaatcagca tcgaggtcat
     6481 tgccgcactg tcgctctgat cgctctccag cccttgtcca cgtagcaaat aagacaatgc
     6541 agcttgttgc gacatcgccg ggtcagagaa gatctccgct ttcggttcgt ccgccagacc
     6601 agtgacgcga actccggcga ttacatcgtc ttctgtagca tccgggttac gaatagcttc
     6661 aatattaaga tacggttgat ccggcggacc agagaacagt aactcaccct tacgcacaat
     6721 cagatcctga ccataggcat ggaagcgccc ttcagggatg ttgatctgcc cgttcaggcc
     6781 cagcccttgt ttgtcctgta cgacgttaag atcgcccgtc agccgcgctt tcaggccaaa
     6841 ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg ttactgttaa tcggaatcga
     6901 cgccgttttc ggctcttccg gttgcaggtt atcgttaagc atcaccacat cgctggagac
     6961 gcctactgcg ctttccggca gatcgtgcac cacgatgcgc gcccacggga catccacgcg
     7021 accatcgagg gtaaacaggt ttggtgtagc ctcgaataca acatctggcg atacatccat
     7081 tcgtaccatc ggcggcacgg tgatccgcac tttactgcct ttcgccgtta ctcgtgcccg
     7141 ccagttttca atttggctcc agtcggcatc accgttcagg tagatttcac cctgctgggt
     7201 ccgtactgta ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa gctggctcgg
     7261 ctgcatatca aacggcataa agttgccgtc gatatccaca cccgtaacct gaagctgacc
     7321 aaacaactgc gggctttgca catcaccacc cagacgcaag ttggcactca ccatccccgc
     7381 tgctttttcc ccacgggtaa agatggggtt tatcatcgca aggttgaagt tacggatatt
     7441 gacgttgcca ccaagattac ggcggccttg cggatcggtc acctgcacct gtccatcaaa
     7501 ctggccgtta ttggtcaggc ggatggtcca gcccaattcg gcacggttgt tacgcaattc
     7561 cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga cggtttgcgt
     7621 tacctgcacg ttacgccccg aaagggtgat actgccctgc ggcagcccct ctttcgtggt
     7681 gtcccaggca acatccgctt tacccgtgaa gataccgctg gcctgagtgg tttctggcat
     7741 aaacggtttc agcatggcga ggtcgaagcg gttgagattc accaccgcac gcccttcggc
     7801 ccccgcatcg atagtttgcg gcacgcacag ttccgcattc gggttaagcc agcagtgtgg
     7861 cccgatgctg attttttgct ccttattgcg gtaatccagc gcaatatcgc gggtcagcga
     7921 ccacgggccg accggcgtct ggaagcgggt attgctaaga gttcccttcc agcgttcttc
     7981 tttgcgatca aaacttcctg ccagattaag ctgcccggag acaggctcgc cctgaatccg
     8041 caactgtagc tcgtgctgct tttcgctgcc tttggcattc agggtgacga ggttgatatt
     8101 tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga tctgatcggt
     8161 ggatttaatg tcgccttcca cgcgaacctg cgccacggaa agttcctgcc agcgcaggcc
     8221 gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc gtgccgcgta ctttcaccag
     8281 ccctttcgct gtaccgccaa gccccggcag cgcgttatcc agccccggcg cgttgatggt
     8341 ggcatcaaga ttgagatctt ttacccccag ctcgcctttc acttcggcac tgtttggccc
     8401 gagttccaga tgaagcccag ggatcatcca ctgcatataa ctgttgcctt tcagcgtgcc
     8461 gtcaacgttc actttgttct gtttgacgtt accggtcagc ttcagttctg gcacctccat
     8521 ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc aagccattga gtttcgacgg
     8581 ccactccggg aactctttgg cggtgtaaat gccattaagc gttagctcac cgcgccaact
     8641 aatggcctgc tgccagtcga gcaacgcctt gagttcagtt ttcccttcca gcgccgcgac
     8701 ggtgagtttg tcgagattga cctgctgttc attacctttg gcatcaaggg taatggtggc
     8761 tggcgggatc tcctgtccct tcactgccgt acgcatagag agcgtgtaat cggtcatttt
     8821 gccggtaagt ttcagtttca gatcatccgc ctgatactgc ttctcaccag tgaacggcca
     8881 gtaaagctgt ttgctgttca cttccacgtt gagtggcaat ccggcttccg ccagtcgcgt
     8941 ctgggcgcgt aaatccatat ccaccggacc ggaaaggtta acgcctatct ccagctgttc
     9001 gcgcagcgcg ccgcccactt tcagcttcac cttttcacct ttcaacggct ccacgttcag
     9061 tgtgctgttg agggtgatat ccaccggcca gttgtctgac agctgcgccg tgccgctggc
     9121 gttgacgatc ccctggttgg aatcgatatc caaggcgtcc agtttagtat tgccgtcaat
     9181 gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg tcacgcgcag
     9241 ctgctcgccc ttaaactctt caatgttcag gttaagcggc aaatgcacgt cggtcatttc
     9301 cggcaatacc gggcgagaaa agagatcttt cagcgtttcg ccgagcggct tttcctccgg
     9361 ctgcggattt tcaattttcg gttcgaccac ttcttcctgc gccacttcca ccactttcgg
     9421 cagagcaatc agcaggcctt tcagcgacgt cggtttcagg gtcagggttt tctcctgcca
     9481 gttcaggccg gaggtgaagt ccatcaccga caccgtggtg tcatcaatct tgatgttgac
     9541 gttgtccagc gccacccgtg tcagggtgat gggatacggc gtggagagat ccagcggacc
     9601 gctatcttcc tcttcttcaa cctgttcaga aggaggcatt tttttactgt cgatgttgac
     9661 ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta ctgttccaca ggcactcaag
     9721 accgaccgcc agatgcagat tacccgcttt taccgccacg cctagctgct cataacgaac
     9781 gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca gtcctggcac
     9841 ccagcgatcc gccgctttaa atacaagatg caggccgctg gtggtgccca ccagaaacgc
     9901 caccgatccc agcaacagta agataacgat aaccacgccg aggctgattt ttttccataa
     9961 actcataatt ctggccccag accgatgtaa aactgtaacc cgtgttcgtc tttatccgcg
    10021 accggtacgg caaaatcgag tttgattggc ccgaccggcg attcccagcg tacgccgacc
    10081 ccggtaccgg ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc actatcgaca
    10141 aacaccgcgc cccaccattt tccggtcacg ttgtactggt attccagcga tccggttatc
    10201 aacttcgagg cccctttcag gtcaccgttg gcgtatttcg gggcgataga tttgtatttg
    10261 tagccgcgaa tactgcgatc gcccccgacg aagaaacgca gatccggcgg tactttgtcg
    10321 aaatcaccgg tttcaatcca gcccagcgta ccgcgtgtca caaagcgatg gcgatcgtac
    10381 agtgtgcgga tccagacgtt ctgcgcctgg aaaacggaga aatcgacatc tgaaccccag
    10441 gccgtgttgg agtagtcgat agagtagcgt tgcgagtctc cccaggttgg catcaggcca
    10501 ccacgagaac gcgtgcggct aatcatcacc ccaggataaa acagcatcgt ggtgttggta
    10561 atttcaccct gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg ctgccagccg
    10621 ctggagagat cccagtagcg agaagccacc agcgtagtgg agtcagattc ggtatcgttc
    10681 aggtcagtgc gcttaaaacc gccctgcacc aaataatatt gttccagtgg attcttcagc
    10741 agcggcattt tatagctgaa gtcgagggtc tgttccggcg cggaaatact ggtactggtg
    10801 gtcaaactgt gaccgtatga gttcatccac ggctttttcc acgtcgcttt cacgcgcggt
    10861 cccacgtccg tagagtaacc gcccccggtt tcgatggtgt tttctgttcg cggcgaaacc
    10921 acgcccgtca atggtaatac tttcgtttcg cgcgctttat caaattgtgg agccaccacc
    10981 accgagttaa accagccggt agcagaaagt cggcggttca gttccgccaa atctttcgat
    11041 tcatactcat cgccctcttt aaacggcacc agattttgca ggtattcatc gcggatttgt
    11101 gatccttcaa aggtcacatg cccaaagcgg taacgttcgc cactgttata atcaatatcc
    11161 cagaaggctt tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa ttcgctatcg
    11221 aaataacctt tacgcaacgc aatactggtt aaggactttt tgaaattttc ataatcgccc
    11281 tggttcagta ccgtaccaat agccgggcga gtatcgagca atttcaaata gtctttatcg
    11341 gtccgcgcgc cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg cacacctggc
    11401 gtgactttgg cgatcaatac ctgccgccct ttctttggcg gtggacggag atcaaattca
    11461 atggtcggct ggtaataacc cagcgctttc agaccttcac ggatggcatc atcgacgcgt
    11521 gcgcgaaagc gacggtctgg cgtcacttca tcactttcaa tcgtagaaag ctgcgcacga
    11581 acgttctttt ccagctgtcc cgataacccc tcgacctgta gacggacgtt cgcggcgacg
    11641 gcagatccgc ttaagcagag taagcttaca cagcataact gtcggatata gcgcacattt
    11701 tctcctgaat atcctttttt cctgcccctg gaacgccgtt aaacggctta acaaaaatcc
    11761 agtaatatgg attaaaaagc agactaaacc ccaaatattt cttatgtttt actttagacc
    11821 tattcacggt ggttattgtg tgcaaatacg cctcttgtta caaccttaac cccaatgacc
    11881 gattttcggg agagcgacac catgagttta tttgataaaa agcatctggt ttcccccgcc
    11941 gatgccctgc ctggacgtaa caccccgatg cccgtagcca cgctgcatgc ggtcaacggt
    12001 cactcaatga ccaatgtacc tgacggaatg gagattgcca tttttgcgat gggttgtttc
    12061 tggggtgtgg agcgtctgtt ctggcagtta cccggccttt acagcaccgc cgcaggctat
    12121 accggcggct atacgccaaa tccgacttat cgggaagtgt gctccggtga tacgggtcat
    12181 gccgaagcgg tacgcattgt ttacgatcct tccgtcatca gctatgagca gttgctacag
    12241 gtattttggg agaatcacga tcccgcccag ggaatgcgtc agggcaatga ccacggcacg
    12301 cagtatcgtt cagcgattta tccgctgacc ccagaacagg atgccgcagc tcgcgccagt
    12361 ctggaacgtt ttcaggcggc gatgcttgcc gccgatgacg atcgtcacat caccacggaa
    12421 atcgctaacg ccacaccgtt ttattatgcc gaagatgacc accagcaata tctgcataaa
    12481 aacccatatg gttactgtgg aattggcgga attggcgtct gtctgccgcc ggaagcatag
    12541 cgttacgggt acaaatgtag tttgttgata aagtgctctt tgtctatgcc tgatgcggct
    12601 taaacgactt accaggccta caaaatcgtc caaattcaat atattgcaag gactgcgtag
    12661 gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga attttaaccc
    12721 tctggcgact ttacagtacc ttacgctata ctagcgactg aaaatgccgg ttcactttct
    12781 tcgaatcggc tttcaatgtg tatttcacac aaattaatca actttccctt ccgaggatct
    12841 ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct gcttgatcgc
    12901 tgtaagtgcg ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca aaatcaaact
    12961 taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata tgcaggaaaa
    13021 tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga ttctcggcgg
    13081 tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc gctatatgtc
    13141 ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga ctggcatgtt
    13201 tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag aagcggtggc
    13261 tttgcgtatc atcaacccga tgcgcttctg cctgtacgtt tgcaccccgc tggtgtggtt
    13321 cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg tacgtaaaga
    13381 tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg cgggcgtgtt
    13441 acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc gtaccgttcc
    13501 gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag atgagcaaag
    13561 cctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta atgaagatat
    13621 tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc tggctaacca
    13681 aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc cggatacgtt
    13741 aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg cggtgatcat
    13801 gaacgagtac gcgctggtgg tggggatcat caccctcaac gacgtgatga ccacgctgat
    13861 gggcgatctg gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg agaactcatg
    13921 gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata ttgacgagtt
    13981 cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc tgcgtaagat
    14041 cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg tggatatcga
    14101 taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca ccgccctttc
    14161 gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc cagaaacatc
    14221 aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta agccatctct
    14281 gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg cagcttatcc
    14341 ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac gtccttgatg
    14401 tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg gttgtaccgt
    14461 tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc cacaatcggc
    14521 ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc gggcaatgtt
    14581 tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta tctttttacc
    14641 gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct tgaccgcaaa
    14701 ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac tcagacccaa
    14761 gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc aagattctgg
    14821 cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa atcggtcagc
    14881 gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa gatcagttta
    14941 gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gcgagtactg caacatattg
    15001 ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt aaatcagcga
    15061 agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac gcgattccgg
    15121 gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat cccaggtcgc
    15181 agtttattgt cgatagcaat ggcgtcgcgc gcggtgcctg gcagctggat gacgaaagtt
    15241 ccgctgtagt ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac ggggcgctca
    15301 caccggaaga ggtgcagcaa gtgatggacc tgctgcaaaa gttgctcaaa taatggcaat
    15361 aacataaatt ctggaaaaat aattttcccc tatcgttatc tatgggatag gggaattaag
    15421 tctctgagtt cttacaccga actaatactg gtaacgctag ttgctctttt ttaaacgcct
    15481 caatgatcga tttatatttc tcgcaaatct tttctttggt aatattacta tctgcggcca
    15541 gattcgcact tactatcgca tccacttgtt cggctaattc gacttgttgg caacctgcat
    15601 taaccagaaa attataaata agagagtaat cttcatcctc caaaacaaca gaggaaccat
    15661 tacgaaaacg ttcatcccct ccatagattt taagcgcaaa gcccaaagat tctatgacta
    15721 tggcaa
//
LOCUS       DAJTNM010000090        15422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTNM010000090 DAJTNM010000000
VERSION     DAJTNM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.090"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..409
                     /locus_tag="OO172_002909"
     CDS             <1..409
                     /locus_tag="OO172_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5513028.1"
                     /translation="FVAQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQ
                     SRVYARAHHYYHSWMHYFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEE
                     LAAGSDRFYATLPWQPLTVQPECPVTLKDVSDL"
     gene            406..1857
                     /gene="djlC"
                     /locus_tag="OO172_002910"
     CDS             406..1857
                     /gene="djlC"
                     /locus_tag="OO172_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5513029.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(1917..3587)
                     /gene="hscC"
                     /locus_tag="OO172_002911"
     CDS             complement(1917..3587)
                     /gene="hscC"
                     /locus_tag="OO172_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR5513030.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(3671..4606)
                     /gene="rihA"
                     /locus_tag="OO172_002912"
     CDS             complement(3671..4606)
                     /gene="rihA"
                     /locus_tag="OO172_002912"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5513031.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            complement(4724..5449)
                     /gene="gltL"
                     /locus_tag="OO172_002913"
     CDS             complement(4724..5449)
                     /gene="gltL"
                     /locus_tag="OO172_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5513032.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5449..6123)
                     /gene="gltK"
                     /locus_tag="OO172_002914"
     CDS             complement(5449..6123)
                     /gene="gltK"
                     /locus_tag="OO172_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5513033.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6123..6863)
                     /gene="gltJ"
                     /locus_tag="OO172_002915"
                     /pseudo
     CDS             complement(6123..6863)
                     /gene="gltJ"
                     /locus_tag="OO172_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015698156.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
     gene            complement(7033..7940)
                     /gene="gltI"
                     /locus_tag="OO172_002916"
                     /pseudo
     CDS             complement(7033..7940)
                     /gene="gltI"
                     /locus_tag="OO172_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            complement(8337..9875)
                     /gene="lnt"
                     /locus_tag="OO172_002917"
     CDS             complement(8337..9875)
                     /gene="lnt"
                     /locus_tag="OO172_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5513034.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(9900..10778)
                     /gene="corC"
                     /locus_tag="OO172_002918"
     CDS             complement(9900..10778)
                     /gene="corC"
                     /locus_tag="OO172_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5513035.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(10868..11335)
                     /gene="ybeY"
                     /locus_tag="OO172_002919"
     CDS             complement(10868..11335)
                     /gene="ybeY"
                     /locus_tag="OO172_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5513036.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11332..12372)
                     /locus_tag="OO172_002920"
     CDS             complement(11332..12372)
                     /locus_tag="OO172_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5513037.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAREEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(12525..13949)
                     /gene="miaB"
                     /locus_tag="OO172_002921"
     CDS             complement(12525..13949)
                     /gene="miaB"
                     /locus_tag="OO172_002921"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5513038.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14095..15270
                     /gene="ubiF"
                     /locus_tag="OO172_002922"
     CDS             14095..15270
                     /gene="ubiF"
                     /locus_tag="OO172_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5513039.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         3728 a         3960 c         4016 g         3718 t
ORIGIN      
        1 attcgttgcc caaaccgcta tgtgctgtgg agaaggggga atcaaagcct gggattatgt
       61 tcgaatgggg tttctgtcac gggccggtgt gctcaacaac tggctgacgg aagaagagag
      121 tttatggctg caatcgcgcg tctatgcacg agctcaccat tactatcaca gctggatgca
      181 ctatttttct gcctattcac tagggcgtct ctactggcaa tcttctcagt gcgaagacaa
      241 cgcttcgcta cgcgaggcgc taaccctgta caaatacgat agcgccggga gtcggatgtt
      301 cgaagagctg gcagcgggaa gcgaccgttt ctatgccacg cttccctggc agccattaac
      361 tgtccagcct gaatgcccgg taacgcttaa ggatgtgagc gacctatgaa aacatgttgg
      421 caaatcctcg aaattgaaag cacgacgcaa atagacatta tccgccaggc ttatcttgct
      481 cgcttaccgt tgtgtcatcc cgaaaccgat ccgcaggggt ttaaagcatt acgccaggcc
      541 tatgaagagg cgctgcgact ggcggtaaat cctgtcgagg aagcagatga tgaagaaaaa
      601 gatgccaccg ctgaacatga aatactacgt gcattcagga cattactgga ttcagaaagt
      661 gatcgttttc agccctccgc ctggcagaaa tttattcagc aattaaatac ctggaacatg
      721 gaggatgtcg atcaattacg ctggccgctg tgtgcaatcg ctatagaagc gcgatatctg
      781 tcattaaatt gtgcttcttt gttggcagag cgtttgaact ggcattcgtt taatgacagc
      841 gaaggaatgg atgaggaaga aagggaggct tttcttgagg ccattcaggc tggtgattgt
      901 ttcgatttcc ttagccttct ggaatatccc gtcgcgttac agaaccagac tgttgaatat
      961 tacttcgcgc tggaacgttg ctgccgttac catcctgact atgtcactgc gtttttggcg
     1021 atggaaggac cgtggttcat tcctgatgat gcaaagttac atcgcaaact gttgcgctgg
     1081 tacagctcgg tgcaaacagg tatggtggaa ctcattcctg tagccaaaca gtggcaagcg
     1141 gaagaaccag aaagcgaaga tgcccggtat taccagtgtg cacaacgttt gtactgcggc
     1201 gagggggaaa gcctgcttgc tgatctcggc gcgtactggg aaagttaccc atctacacaa
     1261 gctgataatt tgttgttgca gtggagcaag cgtcattgcc cggattattt cgcgttatta
     1321 gtgatggtta tcgaagcgcg gagcatggta gatgcgcaag gtcaaccgct gaaatatgtt
     1381 cctggtgaga gcgcccggac gcggctgtta tgggcggaaa ttttacatag cggaaaatta
     1441 tcgccgttag gtcaatcgtt tattgagtcg ttattcttca aacgcaaagc atgggcgtgg
     1501 tggaaatcga gagtcggtag cgagacagaa gaagattcac cgttactgga tttgtatcgg
     1561 gtagcggaac aggtagtact tgaagcgttt ccgaaacaag agatgctggc ccgtcttaat
     1621 acaaggctgg aaggcggaga tgctcatcca ttagaggcca ttatcacccg gatgctgttg
     1681 acgaaagtaa aactcgagcc ggaggatgaa gatgtcgatg agccaacacc tgaaaaccat
     1741 gaagaaaaaa atgatgaggg tgaaaaacca cagagcatta ccagcattat caaaatcagt
     1801 ttaacggtgc tggttatagg ttatgctctc ggcaaaatcg cgatgttgtt tagttgacga
     1861 tgtgtttttt gcgccgatgc cggatgcagc atttacgccg catccggcag ccgtgattat
     1921 gaggtctcaa tggctaaata aacgctcgcc cgccgcctga cctcatcgac gcgggccata
     1981 tcgtttgact gcagcgccgc atcaaaatcg gtgatcaccc ggccaatctc ttctcgctcg
     2041 tcacccagcg cccgcgccca caactcttcc agtttggctt taaaggtgcg attgatgagc
     2101 atatcgcgcg gataaatttt caatgcggat aaccgcgtcc gactctcttc aatctgctgc
     2161 gcgctcaatg ttaccgggct gtggttaatc actctggact taacgctgcc gtcttccaga
     2221 agcacgtcaa cttcaagcaa cccgttgata tcataactaa agcgaatatc aatcgactga
     2281 taagccccgg ttttcttcaa cggcacatcg aaggattcca ccagaatgtt gtttttaact
     2341 ttgtggtttt ctccctgata gacgttaacc gtaattgaat cctgttccgg gtgcatggtt
     2401 gaataagttt ctacacgcga cacgggcaca gtggtgtttc gttcaataat cggcgagaaa
     2461 atgccggaaa cgccctggcg gttaacctca acgcccaacg agtaagggca aatatcagtg
     2521 aggatcactt cttcaatatc ttcactgcgt aagcggcagg cggcctggat tgctgcgccc
     2581 agcgcgacaa tggtgctcgg atcgtaactt tgatacggta atttgccaaa cagacgcacg
     2641 gcgattcgct gcaccagcgg catttgtgac gcgccgccaa ccagcaccag actgtcgatt
     2701 tgactcggct tcagacgcgc atcgcgcaac gcctgttcaa tcggcactcg caagcgattg
     2761 agcagcggca accacaaatc ttccagttcg ttctcgtaaa attcgcattc ccgcgtttca
     2821 tcctggtact gccagcgaat gtgcattggc gattgattgc tacattttgc cgcttccaca
     2881 caggcgtaca aggctgccag ttcactctcg ttaagcgtgg tccttgtgac atccgcgcgc
     2941 tttaaaacgt catcgaccaa catatgggta aaatcttcgc caccgaggaa gttgtcgcca
     3001 gcggaggcgt gaacttcaat caccggcgtg gcgtactcaa gcaccgtaac gtcaaacgtg
     3061 ccgccaccga gatcaaacac cagcgaacgg gtattttgtt gggtatgcag gccatacgcc
     3121 atcgcagctg ctgtgggttc attaattaag cgtaccgcat ttaacccggc taagtccgct
     3181 gctaaacggg tatgcttgcg ttgtttatcg ctgaaataag ccggaacgga gatcaccaca
     3241 tctttaatcg gacgttgcag aaattcttcg gcatcttctt ttaatgagcg taataccaaa
     3301 gaggacagtt ctggcgcgtt aaatgtgtcg ctgcctaacc gccagttggt attactgccc
     3361 attgcacgtt taaataacgc tgccgtttta tccggatgcg aagtacgccg tgatacagcc
     3421 ggttttccga ctaaaatatg attattttca tccatgctaa ttatggatgg tgttaaatat
     3481 tcaccgaact tatttggaat taattgcgcg gcaccgtctt tccagacggc aattaaacta
     3541 ttggtagtgc cgagatcgat acctatggcg agttctgcat tatccattgc atttatatct
     3601 tgaaagaaaa agagataaat agattaagac cacccaggta acgcgtggtg atcttaatca
     3661 atgacgagtg ttaagcgtaa aatttcagac gatcagccag taaatcaaca aagccctgac
     3721 gatcaacatc gacaattaca gtggcattcg gtttattgcc agtcagataa taataatcaa
     3781 caaccgtcat acccagggta tatttcccct gtgtttccac gccaacccag cgctcaacag
     3841 tggtaaacaa ctcgggtttc aacagccagg cgatggtgca tgggtcatgc agcggtgcgc
     3901 cgacaaagcc ccatttttcg tctttatgat attcgaggaa gaaatccagc agttcggcaa
     3961 caatggttga aacagggttg ccaatcgcgc ggaaacgctc ggtgtcttca acgtggattt
     4021 gcgctttatg agtaacatcc agaccggcca tcaccaccgg gatccctgac tggaagacaa
     4081 tttctgccgc ttccgggtca acgtaaatat taaattcagc cgcaggcgtc cagttaccaa
     4141 gccccattgc gccgcccata atcacgatac gggcaatttt gctatgcagt tccggatggc
     4201 tattgagcag caaggcaacg ttagtttgcg gtccggtaga aacaatcgtg acaggttccg
     4261 cactttcgcg cagcgttttc gccatcagct ccaccgccgt acagttttgc ggtgcgaacg
     4321 tcggttccgg taatgccggg ccgtcgagac cgctttcgcc gtgcacattg tccgcgataa
     4381 tcaactcacg cattaacggt tttaccgcgc cgcctgccac cggaatatcg gtgcgattaa
     4441 gcaaggtcag catacgcaga acattgcgta aggttttttc tggtgtctgg tttccggcgg
     4501 aagacgtaat tgctttgaca tcaagctctg gtgaggcgag ggcgagaact attgcgatag
     4561 cgtcatcatg acctgggtcg caatctaaca gaattggcag tgccattgtt gctccttgtt
     4621 gtgtgcttct ttgcgacaag ggtaacgcca ggatgtaaca gatacgaggg gcgaaacgat
     4681 aaagcgtgag atggcgcgca attgggtatg cgcgccagag tgattaatgc aggattttcg
     4741 cgaggaagtc ttttgcgcgg tccgatttcg gatcatcgaa gaaagcgtct ttcggcgagt
     4801 cttcgacaat tttaccctcg tccataaaga tcacccgatt cgccactttg cgggcaaagc
     4861 ccatttcgtg ggtcaccacc atcatggtca ttccttcgtt cgccagttcc accatcacgt
     4921 ccagcacttc gttgatcatc tccggatcca gcgccgatgt cggttcgtcg aacagcatcg
     4981 caataggagc catacacaat gcgcgagcga ttgccacacg ctgctgctga ccgccggaaa
     5041 gctgcgccgg aaatttattg gcatgtgcgg aaagtcccac gcgctccagc aatttcaatg
     5101 ctttttcacg agcgggcgct ttatcgcgtt taagcacttt cacctgcgcc agggtcaggt
     5161 tttcgataat cgacagatga gggaacagct cgaaatgctg gaataccatc ccgacgcggg
     5221 agcgcagctt tgccagatcg gttttcttgt cgttaaccac gataccatcg acggtgattt
     5281 caccttgctg cactggttcg aggccgttga cggttttaat cagcgttgat ttgccggaac
     5341 cagacgggcc gcaaaccacc accacttcgc cttttttcac ttcggttgag cagtcggtca
     5401 gcacctgaaa gtgaccatac cattttgaaa catttttcag ggtaatcatt atgctgtcct
     5461 tcttttcaag tagctgacca acaacgacgc gctaaggcta ataacgaaat aaacaaatcc
     5521 ggcaaacagg atcatctcga cctgcgtacc atcacgctca ccaatggttg aggcggtacg
     5581 gaagaaatcg gccagagata acacatacac cagcgaggta tcctggaaca gtacgatgcc
     5641 ctgagtgagc agcagcggca ccatcgcgcg gaaagcctgc ggcagaataa tcagtttcat
     5701 cgactgccag tgagtcatcc ccaacgccag cgcggcgctc gactgaccac gggaaatact
     5761 ttgaataccg gcacggataa tctctgaata gtaggccgct tcaaacatcg aaaacgccac
     5821 catcgccgaa attaaacgaa tatcattttt tggcgataat cccagcacat tttgcagaaa
     5881 tcccggcaca atcaagtaaa accacagcaa aaccatgact aaaggaatcg agcggaatac
     5941 gttaacgtag gctttggcaa accacgaaac aggcgcgaag ctggacaagc gcatcaccgc
     6001 cagcatggta ccccacaaaa taccaatcac gactgccgtg acggtgattt tcagggtgat
     6061 caccagcccg tcgagcagat atggcaggga aggaacaatg gaactccagt caaactcgta
     6121 cattatttgc cccccatgtt gccgggcagg cgaactttac gttcaaccag cgtcatcacc
     6181 agcatgataa aagcgttaat caacacatac gtaagcgtga tggcggtaaa cgactcccag
     6241 gcgtgagccg agtagtcgag caatttaccc gcctgcgccg ccatatccac cagaccgatg
     6301 gtcgaggcga tggcggagtt tttcaccagg ttcatcatct cggaagtcat cggcgggacg
     6361 ataacgcgat aagcattagg cagcagtacg tatcgataag tctgcggtag cgtcaggccc
     6421 atcgccagcg cggcattctt ctgcccacgc ggtagcgact gaatagcggc gcgtacctgt
     6481 tcgcaaacac gggaggcggt aaacagcccc aggcagagca tggatgaaag gaaaaactga
     6541 atattgggat ccagctcggc cttaaaccac atgccgattt tctccggcag cagctccggg
     6601 atcaccagat accaggtaaa gaattgcaca atcaacggca cgttgcggaa cagttcgaca
     6661 tacaacgtac ccagaccgga gagaaaacgg tttggaacgg tacgtaaaat gccgaaaaat
     6721 gaaccgacga ggaaagcgat aattcaggcg cagatcgaca aagcgatcgt cacctgaaaa
     6781 ccactccaga cccaaccaag ataggtggta ttgccgaacg gggcttgttg taaaaaaata
     6841 ccccagttcc agtctataga cataaatcta ctccagaaaa aagagggtag cagcgttaac
     6901 tgctaccctc gaagattgtt acccagcgta ttgcggtttt caggcccgat ggggaacgac
     6961 catcaggcgt atagtctgtc cgtgctacgt aacaatcgag agggctggaa tttccgcctc
     7021 tggttcttgt aattagttca gtgccttgtc attcggttct ttgaacagtg ctttcatttc
     7081 gtctgacagt tcgaaattca tgttcaggtt tttcggcgga attggatttt tgaaccattt
     7141 atcaaaccat ttttccgctt caccggaggt ctgcacctga gcgatagtgt catccatcag
     7201 ctttttgaac tgcggatcgt ctttacgcaa catgcaaccg taggcttcct gagactgcgg
     7261 cttgccgacg atatcccagt tgtctggttt cttcgctttc gcacgttcac cggccagcag
     7321 agcgtcatcc atcataaagg caacggcacg accgctttcc agggtgcgga aagagtcacc
     7381 gtgatctttg gcgctgatga tgcgcatatt cattttttgc tcttcattca gtttgttgag
     7441 caaaacttca gaggtagtgc cggaagtgac gactacggct ttgcctttca ggtcggcaaa
     7501 atctttgata tcgccgccct ttttggtcaa caggcgcgta ccgaccacga aaatagtgtc
     7561 agagaaagcc gcctattttt ggcgttcgac gttgttggtg gtagaaccac attcaaaatc
     7621 gaaagtgccg ttttgcagca gtggaatacg gtttgtgagg taatcggaat cagttttacc
     7681 tgcaagtccg gtttgttgag tttctttttc actgcttcaa caatggcgtt ggagtaatcc
     7741 tgcgagtaac ccaccacttt ttgctgattg tcgtaataag agaaaggtac tgaagattca
     7801 cggtgaccga cgacaatcac accgtttttg gcgattttgt ccagcgtact gcctgctgcc
     7861 ggggcggcgt catctgcctg tgccagtcct gcggaaagcg ccagggcgag gattgctgtg
     7921 gcaggtttac gtaattgcat atccaactcc tttatcttct gcgttaagaa cgcatggata
     7981 cccgttgtga gtgtttgtgt tgttatcgtc tgcaacttta ttgtgcagtg ttgtgcctgt
     8041 taggtaacat ttagtttggc taaatgtaaa gatattgatg ttttattgtt tgtttttgcg
     8101 aggtgcaccg caccattctg aagcaaaatt cttaaaatgc actcttttgg tgctaccggt
     8161 ggattgctgt ggtgcaaccg ggttgtactg atgctggttc aagctttgcc ttgtataaca
     8221 aagcaataga tgtgccaaag ttggatagga gaatattgtt atccggataa tgcactgatg
     8281 ccgcatccgg tgagcgtgcc gaaatatggg atgtattccg gcacgataag aagggattat
     8341 ttacgtcgct gacgcagact catcaacaca gcagcaaaac caaacagtgc cgtcagcacc
     8401 cacagcggcc agttgccggt acgtgcgtat ggtgtgagtc cggtggtcgg cgtcacgtta
     8461 gtggttaaca cctcgcgggt gaactgcggg atcatcgcct gaatctcacc ctgcgggcca
     8521 atcaccgccg taatgccgtt gttggtgctg cgcaacagtg ggcgcgccag ctccagcgca
     8581 cgcattcgcg ccatctggaa gtgttgccat ggaccaatag atttaccaaa ccacgcatcg
     8641 ttggagatag tcagcaggta gtcggtatcc gggcggaagt tatcgcgcac ttgctcgccg
     8701 agaatgatct cgtagcaaat agccgcagta agctcaatac catttgccga cagcggcggc
     8761 tggatatatg gcccacggct gaacgacgac atcggcagat caaagaacgg tgctaacgga
     8821 cgcagaatcg actccagcgg gacaaactcg ccaaacggca ccagatggtt tttgttatag
     8881 cgatcggctg attcgtagct gtacggtgca cctttaccca gcgtgatgat ggtgttgtag
     8941 gtatcgtagc ggttctgctt attgagacgc gcgtcgacaa tcccggttac cagcgagcta
     9001 cctttatcac gcaactcacc gtccagtgct ttgaggaacg gttgctgatt aatttccaga
     9061 tcggttatcg ccgactccgg ccagataatc aacgatgatt tgcccatcag cggtgccgtt
     9121 gcgttgtagt aaatctttag cgtattaaga agctggcctt cgtcccattt cagcgattgc
     9181 ggaatatcgc cctgaaccat cgaaacctga atggttttct ccggttgcgg ggtaaaccat
     9241 tggatgtaac gcagcgggaa gggtagggca aacagcacga cggccaccac cagcggacgc
     9301 cagttgcgtt tgaccaacgc cagtgccagc aggccactaa ccatcatcag caggaagtta
     9361 atggcttcca cgcccattag cggtgccagc ccttttaacg ggccatcaat ctggctatag
     9421 ccgaactgta accacgggaa gccagtcagc acccaaccgc gcagaaactc ggtcacttgc
     9481 cagagagcag gggcggcaat cgccacgcgc agccaggtgg ttttcggcca cagacgcgac
     9541 agcacgccag caaacagtcc ggtatacagc gacaaatacg ccgccagcag caccactagg
     9601 aagatgttaa ccgggccagg catcccgcca aaggtcgcga tgctgacata gacccagtta
     9661 ataccgctgc caaagaggcc aaatccccag caaaagccaa tagcggcaga ctggagtggg
     9721 cgacggttaa aggtcaacgc ctgaagcccc atcagcgaaa taatcgccgc aggccagacg
     9781 tcgtaaggag agaaggccag cgttccgcag gcaccgaata ataacgccag cagcaggcga
     9841 atgcgctggc gttcaattaa tgaggcaaaa gccatgtagt tatctatcca gtttcggttt
     9901 tattcatcca gctttggctg gggtgagtca tccgggattt tgacatgaac ctgaataata
     9961 cgccgactat cggccatcgc cactttgaac tggtaaccgt cgatgtcgat agtttcgcca
    10021 cgcgccggaa gatgcccaaa cgcctgcatc accagaccac cgatagtgtc gacttcttca
    10081 tcgctaaagt gggtgccgaa cgcttcgttg aagtcttcaa tggaagccag tgcgcgcacg
    10141 gtccaggtat gacgactcag ctgacggaag tcgatatcat cttcttcgtc atactcgtct
    10201 tcaatctcac caacaatcag ttccaggatg tcttcaatgg tcaccagacc ggaaacccca
    10261 ccgaattcgt caataacgat cgccatgtgg taacgctgag agcgaaactc tttcagcatc
    10321 cggtctacgc gcttactttc aggaacgaca accgcctgac gtaacacttt gtccatgccg
    10381 aaggcttcag catcgctgcg cataaacggc agcaagtctt tcgccatcag aatcccttca
    10441 atgtgatctt tgtcttcgct aatcaccggg aaacgtgagt gggcggactc gatgatgaca
    10501 tcaaggcatt cgtccagcgt ctggttgcgt ttcagggtaa tcatctggga gcgggggatc
    10561 atgatgtcgc gaacgcgttg gtctgcgatg tccatcaccc cttcgagcat atcgcgcgta
    10621 tcttcgtcga taaggtcgtt ctgcccggaa tcacggatca gcgccagcag ttcgtcacgg
    10681 tttttcggtt caccgtggaa aagttggctg agtaacaggg agaaaaatcc cttcttgttg
    10741 cttatcgtgt cactactgtg tgaattgtcg tcgctcatgg cgtcgtatgg gttctcatgt
    10801 tagttaatca aaacgccgtc gttaatcacc aacggcgggg acgtctgcca gtcaaatgcc
    10861 tggcaaatta ttctttctcg gcaatgtacg gatcctcata gcccagagca agcataatct
    10921 ctgtttcgag ggcttccatt tcttctgctt cgtcatcttc aatgtgatcg taacctaaca
    10981 aatgcagact gccgtgcacc accatatgcg cccagtgcgc ctccagtggt ttgccttgct
    11041 cctgagcttc cttctcaacc acctgacggc agataaccag atcgcccagt agcgacatct
    11101 ccatgccagg cggcacttca aacgggaagg agagcacgtt ggtcggctta tccttaccgc
    11161 ggtaggtcag attcagactg tggctttcgg cggtatctac cacgcgaata gtcacttccg
    11221 actcttcctg aaactgcggg atcaccgcat tcagccatgt ttgaaactgg ctctcttccg
    11281 gtaacccgga attatcttca catgccagtt gtaaatcgag gatcacctga ctcatttttg
    11341 ttcctgttct tcgcgcttgc gttctgctgc cagcgccgct ttacgttttt gttctgcttc
    11401 ttccctggct tcataagcgt taacgatacg cgccaccacc gggtgacgaa ccacgtcttc
    11461 gctgtggaag aagttaaagc tgatctcttc gacatcggcc agcacttcaa tggcatgacg
    11521 taagcctgat ttagtattac gcggcaggtc gatctgtgtg acgtcgccgg tgataaccgc
    11581 ttttgagtta aaaccgatac gggtcaggaa catcttcatc tgttcgatgg tagtgttctg
    11641 gctctcatcg agaatgataa acgcgtcgtt cagcgtacga ccacgcatat aggccagcgg
    11701 cgcgacttca ataacgttgc gctcaatcag tttctcgact ttctcaaagc ccagcatttc
    11761 aaacagcgcg tcgtacagtg ggcgcagata cgggtctact ttctggctta aatcgccagg
    11821 caggaagccc agtttctcac cggcttcgac cgccggacga gtcagcagaa tacggcgaat
    11881 ttcctgacgc tccagggcat caactgccgc agccactgcc aggtaggttt tacccgtacc
    11941 cgccgggcca acgccgaagg taatgtcatg gtcgagaata ttggcgatgt actgcgcctg
    12001 gtttggcgtg cgcggcttaa ttacgccgcg tttagttttg atattgaccg ctttgccgta
    12061 ctccggcacg ctctccgcgc tttgctccag tacccgcgct tctttaatcg caaggtggat
    12121 ctgttccggt tcgatatcct gaatctgacc gcgcatcggg gcagtatcga catacaggct
    12181 acgcagaatg tctgccgcag cggtgacgca aatcggacgg ccggtcagtt taaagtggtt
    12241 atcgcggcga ttgatctcga tgccgagacg gcgttcgagc tgcttgatgt tgtcatcaaa
    12301 cgggccgcac aggctcaaca gacgcgcatt gtctgctggc tccagggtga tttcgcgagt
    12361 gtctatgttc aaaccgttcc tcttatctgt atgccgccgg aagctgaaca ttcaccggcc
    12421 tataaggaaa ttattcacgc cacaggaaaa aggcgcaagc attgcgataa agatggggat
    12481 aaagagagaa aaaacaaggc ccaccggaac ggcaggcctg agaattacgg ctgataataa
    12541 cccacgccaa ggtcgttttc tttgcgggtg cgggcaatca ccgactccgg tgtttctgcc
    12601 atgcgcagac ccatttcatc ttcagttcgc accactttac cgcgcagaga gttcgggtag
    12661 acgtcggtaa tttctacatc gacgaattta ccgatcatat ccggtgtgcc ctcgaagttg
    12721 accacgcggt tattttcggt acgcccggaa agctccatga tgctcttacg cgaagtccct
    12781 tctaccagaa tacgctgggt ggtgccgagc atccgacggc tccatgccat cgcttgctga
    12841 ttgatacgtt cttgcagaat atacagacgc tgcttcttct cttcttccgg aacatcatca
    12901 accatatcgg cggcaggcgt tcctggacgt gcagagaaga taaagctgta gctcatgtcg
    12961 aaattgacgt cggcaatcag cttcatcgtt ttctcgaagt cttcggtggt ttcgccaggg
    13021 aagccaacaa tgaagtcaga actgatctga atatccggac gcgccgcacg cagtttacgg
    13081 atgatcgctt tgtactccag cgccgtatgg gtacgtccca tcaggttcag aatgcgatcg
    13141 gaaccgctct gtaccggcag atgcaggaag ctcaccagct caggcgtgtc gcggtacact
    13201 tcgatgatat cgtcggtgaa ttcgatcgga tggctggtgg taaagcgaat acgatcgatc
    13261 ccgtcgatcg cagcaaccag acgcaacaga tcggcaaacg atccggtggt gccgtcgtag
    13321 ttctcaccac gccaggcatt cacattctga ccgagcaggt tgacttcacg cacgccctga
    13381 gccgcaagct gggcaatctc aaacagaata tcgtcagacg gacggcttac ctcttcacca
    13441 cgggtgtaag gcaccacgca gtaggtgcaa tatttattgc agccttccat gatggagaca
    13501 aacgcggtcg gcccttcggc gcgcggttcc ggcagacggt caaacttctc gatttccggg
    13561 aagctgatat ctacaaccgg gctgcggtcg ccgcgcacgg agttgatcat ctccggcaga
    13621 cggtgcagcg tttgcggccc aaaaataata tcgacatagt gggcgcgctg gcgaatgtgc
    13681 tcgccttctt gcgatgccac gcagccaccg acgccgataa tcaggtctgg attcttctct
    13741 tttaacagtt tccagcgacc caactgatgg aagacttttt cctgagcctt ctcgcggatt
    13801 gagcaggtgt tcagcagtag cacatccgct tcttccgcca cgtcggtcag ttgatagccg
    13861 tgggtggcat ccagcagatc ggccatcttc gatgaatcgt actcgttcat ctgacagccc
    13921 caggttttaa tatggagttt tttggtcatc gacttgctct tgcgaaatag tagccaggaa
    13981 tgcagggcgc atagtgtaat gctttgctgc cgttgtgacc agtatgagcg ttatcagccc
    14041 ttgggggtaa aaatcctgta aacttaaagc atattgctaa caggatgatt gaccatgaca
    14101 aatcaaccaa cggaaattgc cattgtcggc ggaggaatgg tcggcggcgc actggcgctg
    14161 gggctggcac agcacggatt ttcggtaacg gtgatcgagc acgcagaacc tgcaccgttt
    14221 gtcgctgaca gccaaccgga cgtgcggatc tcggcgatca gcgcggcttc ggtatcattg
    14281 cttaaagggg taggggtctg ggatgcagta caggctatgc gttgccatcc ttaccgcaga
    14341 ctggaaacct gggagtggga aacggcacat gtggtatttg acgcagccga attaaagttg
    14401 ccgttgcttg gctacatggt ggaaaacaca gtcctgcaac aggcgttgtg gcaggcgctg
    14461 gaagcgcatc cgaaagtaac gttacgtgtg ccaacctcgc tgattgcatt acatcgagat
    14521 aatgatcttc aggagctgga actgaagggc ggtgaaacga tccgcgcgaa actggtgatt
    14581 ggtgccgacg gcgcaaattc tcaggtgcgg cagatggcgg gaattggcgt tcatgcctgg
    14641 cagtatgcgc agtcgtgcat gttgattagc gtacagtgtg agaacgatcc cggcgacagc
    14701 acctggcagc aatttacccc ggacggaccg cgtgcgtttc tgccgttgtt tgataactgg
    14761 gcatcgctgg tgtggtatga ctctccggcg cgtattcgcc agttgcagaa tatgaatatg
    14821 gcgcaactcc aggcggaaat cgcgaagcat ttcccgtcgc gtctgggtta cgttacaccg
    14881 cttgccgctg gtgcgtttcc gctgacgcgc cgacatgcgt tgcagtatgt gcagccgggg
    14941 cttgcgctgg tgggcgatgc cgcgcatacc atccatccgc tggcggggca gggggtgaat
    15001 cttggttatc gtgatgtcga tgccctgatt gatgttctgg tgaatgcccg cagctacggt
    15061 gaagcgtggg ccagttatcc tgtcctcaag cgttaccaga tgcggcgcat ggcggataac
    15121 ttcattatgc aaagtggtat ggatctgttt tatgccggat tcagtaataa tctgccgccg
    15181 ctgcgttttg tgcgtaatct cgggttaatg gcggcagagc gtgctggcgt gttgaaacgt
    15241 caggcgctga aatatgcgtt agggttgtag ccttacaaca ttgccggatg cgtgccaacc
    15301 gtaggtcgga taagacgcgg cagcgtcgca tccgacattg aaggataaga cgtgtcaaca
    15361 tcgcattcga cattgaatga acgcagaaaa gcaaaaagct cgccgaagcg agctttttta
    15421 at
//
LOCUS       DAJTNM010000091        15187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTNM010000091 DAJTNM010000000
VERSION     DAJTNM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.091"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..493)
                     /locus_tag="OO172_002923"
     CDS             complement(<1..493)
                     /locus_tag="OO172_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR5513040.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATDH"
     gene            complement(619..924)
                     /locus_tag="OO172_002924"
     CDS             complement(619..924)
                     /locus_tag="OO172_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5513041.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(1032..1108)
                     /locus_tag="OO172_002925"
     tRNA            complement(1032..1108)
                     /locus_tag="OO172_002925"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1072..1074),aa:Val,seq:gac)
     gene            complement(1113..1189)
                     /locus_tag="OO172_002926"
     tRNA            complement(1113..1189)
                     /locus_tag="OO172_002926"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1153..1155),aa:Val,seq:gac)
     gene            1497..2753
                     /locus_tag="OO172_002927"
     CDS             1497..2753
                     /locus_tag="OO172_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5513042.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(2794..4167)
                     /gene="mdtK"
                     /locus_tag="OO172_002928"
     CDS             complement(2794..4167)
                     /gene="mdtK"
                     /locus_tag="OO172_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5513043.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            4382..5023
                     /gene="ribE"
                     /locus_tag="OO172_002929"
     CDS             4382..5023
                     /gene="ribE"
                     /locus_tag="OO172_002929"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5513044.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(5063..6211)
                     /gene="cfa"
                     /locus_tag="OO172_002930"
     CDS             complement(5063..6211)
                     /gene="cfa"
                     /locus_tag="OO172_002930"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5513045.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(6502..7713)
                     /locus_tag="OO172_002931"
     CDS             complement(6502..7713)
                     /locus_tag="OO172_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5513046.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            7826..8758
                     /locus_tag="OO172_002932"
     CDS             7826..8758
                     /locus_tag="OO172_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5513047.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(8755..9780)
                     /gene="purR"
                     /locus_tag="OO172_002933"
     CDS             complement(8755..9780)
                     /gene="purR"
                     /locus_tag="OO172_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5513048.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            10079..10168
                     /gene="ynhF"
                     /locus_tag="OO172_002934"
     CDS             10079..10168
                     /gene="ynhF"
                     /locus_tag="OO172_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5513049.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            10334..11503
                     /locus_tag="OO172_002935"
     CDS             10334..11503
                     /locus_tag="OO172_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5513050.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(11649..12230)
                     /gene="sodB"
                     /locus_tag="OO172_002936"
     CDS             complement(11649..12230)
                     /gene="sodB"
                     /locus_tag="OO172_002936"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5513051.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(12358..13185)
                     /gene="mepH"
                     /locus_tag="OO172_002937"
     CDS             complement(12358..13185)
                     /gene="mepH"
                     /locus_tag="OO172_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5513052.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            13520..13867
                     /gene="grxD"
                     /locus_tag="OO172_002938"
     CDS             13520..13867
                     /gene="grxD"
                     /locus_tag="OO172_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5513053.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(13906..14406)
                     /gene="tnpA"
                     /locus_tag="OO172_002939"
     CDS             complement(13906..14406)
                     /gene="tnpA"
                     /locus_tag="OO172_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5513054.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            14414..>15187
                     /locus_tag="OO172_002940"
     CDS             14414..>15187
                     /locus_tag="OO172_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5513055.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQAESEVSTPVHPSASMVGLDAGVAKLASLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVS"
BASE COUNT         3960 a         3609 c         3906 g         3712 t
ORIGIN      
        1 cgtgatccgt ggcaatcacc gctaaatcaa acgtctcgct gggtaaatcc tgatttgtag
       61 cgagcatgac gccagcattt gtaatttgca gatcggtaac ctggcatgat tcataaacag
      121 ccactgcaaa tttttgctgt cgtgcctggt ctactaatcg taaaaattga tcgcggaaat
      181 attcgcccag cagaattcgc ggtaaaaact gacgatcgtg caaggtttct tttttaacgc
      241 cataacgctg gagatggctg gcttcttgct tttgtagcca ttcgagatac gtacaattaa
      301 tcggcggtat ttcaatactg gcaatatttg ccagcatcat ttttgagttt tcctcatcac
      361 tgtatggcat cccgacaccg gcctcgtcag cctgctcgaa gatagaaata gaaagtggag
      421 tttgttgctg tagaagcgag aataaggtgt agatccccgt aggcccggca cccacaatag
      481 caattttttt cattgttact tacctgtacg cattctttcg aatttagtgt tatcagataa
      541 gtttggttga gatttttaga atttgagaaa tttcagacag ggatgaaaag ggcgctaacc
      601 gcccttgatt aaattctgtc aggcgagttt tgcctgattg atttgactaa gtggctcatt
      661 gacatcgaaa actttggcga caacttcctc aacgccgaga tttttcagtc gggcagtatg
      721 tttttccaga taggcaaggg cgcttttttc atcagtgaaa aggtagatcc caccggcttc
      781 gtggttcttt tcactttctg tccatacctt ccacagaaaa ccaggttcct gattaatcga
      841 ctcagcaagt ggtttaagct gctcagccat tgcgtcgcca aatgggccat taaaagcaaa
      901 atgaaattgt aacaaagttg ccatagcgat tctccttctg ataacgcata cgaagacagt
      961 gtaaatcttt taacgtattg gatacagaga agaaacagag ggaaaaagga aaagaccaga
     1021 ttaagaaaat ctggtgcgtc cgagtggact cgaaccaccg acccccacca tgtcaaggtg
     1081 gtgctctaac caactgagct acggacgcag gatggtgcgt tcaattggac tcgaaccaac
     1141 gacccccacc atgtcaaggt ggtgctctaa ccaactgagc tatgaacgca acgttgtagg
     1201 tgacaacggg gacgaatatt agcggcagag tggaaaggtg gcaagaggca aaacgtaatt
     1261 ttctgcgcta tttcgaccgt ttgcagagct tttaagcaaa ttggctatat tttgttgatt
     1321 tgcaagggcg atttttattc aggatcgcat ttacatctga tacaacccaa taattcgtgt
     1381 atcagagttt aaacagagtt gtttgccacc atctcaaatc tgtcgtggat aaatcctccg
     1441 tagcctttac acttaggtag caataaaggc acacagatgc agcaaaggaa taaatcatgg
     1501 gatctgatgc gaaaaacttg atgagcgacg ggaatgtgca aattgttaag accggcgagg
     1561 tcattggcgc gacgcaactt actgaaggcg agttgattgt tgaagctggc ggaagagccg
     1621 aaaataccgt ggtcacgggg gctggctggt tgaaagtggc aaccggtggg atcgccaaat
     1681 gcacacagta cggtaacaat ggcacgctat cggtcagcga cggtgccatt gccacagata
     1741 ttgttcagtc cgagggaggc gcaattagtc tctctacgct cgctacggtt aatggccgcc
     1801 atcccgaagg tgaattcagc gttgatcagg gttatgccta cggtttgttg ctggaaaatg
     1861 gcggtaacct gcgtgtactg gaaggacatc gcgcggaaaa aatcattctc gatcaagagg
     1921 gcggcctgtt ggttaatggg acaacctcag cggtcgtggt agatgaaggt ggtgaattgt
     1981 tggtgtatcc aagtggagaa gccagcaatt gtgagattaa tcagggcggc gtttttatgc
     2041 tggccgggaa agccagtgat acgttgcttg ctggtggcac catgaataat ctcggtggtg
     2101 aagactctga cactattgtt gagaatggat ccatttatcg tctggggacg gatggccttc
     2161 agctctacag ttccggtaag acgcaaaacc tgtcggtgaa tgtgggggat cgggctgaag
     2221 tgcatgccgg tacgctggaa aatgcggtaa tacaaggtgg aacagtgatc ctgttgtcac
     2281 ccaccagcgc ggacgaaaat tttgtcgtag aggaagatcg cgcaccggtt gaactgaccg
     2341 ggagtgttgc attactggac ggcgcttcaa tgattattgg ttatggcgca gagctgcaac
     2401 aatcaacgat tactgtacag cagggcggtg tgttgattct cgacggcagt acggtaaaag
     2461 gtgacagcgt cactttcatt gttggtaaca tcaatctgaa tggcggaaaa ctgtggctga
     2521 tcactggtgc ggcaacgcat gtgcaactga aagtgaaacg cctgcgcgga gagggagcga
     2581 tttgcctgca aaccagtgcg aaagaaattt cacctgactt catcaatgtg aaaggggaag
     2641 ttaacgggga tatacgcgtt gagataacag atgccagtcg gcaaactctg tgtaacgctc
     2701 tgaaattaca gccagacgaa gacgggattg gcgcaacgct ccagcctgcg taaaaacaca
     2761 ggcgcaaccc gtgaaggttg cgccttgtct ttattagcgg gacgctcgtt gcagaatgat
     2821 ggctgacggc atacgttgca ggaaccgcat acgcagcatc atcataatgg ctgccgatgt
     2881 caggccgata ataaagccta tccagaagcc tgctggcccc ataggttcaa cgaccagatc
     2941 ggtcagtgcc agaatatagc cgcttggcaa gcccagcacc cagtaagctg taaaggtgat
     3001 atagaaaatg gaacgcgtat ctttataacc acgcaaaatc ccactgccaa tcacctggat
     3061 tgagtcagaa atctgatata ccgccgccag caacatcaaa tgcgcagcca gcgttacaac
     3121 ctcgggattg tcgttgtaca acagggcgat ttgctcccgc agtgaaaccg tgaaaatggc
     3181 cgtcagggtt gccatacaga cacccaccat aagcccggtc cgcgcagcgg tttgcgcatc
     3241 cagcgttgag ccctgaccca gacgataacc tacgcggata gttaccgctg ccgccagcga
     3301 cattggaagc acaaacatca gtgaactaaa gttcagggca atctggtgtc ctgcgacatc
     3361 aacaataccg agcggagaca ctaacagagc cacgacggca aacagtgtca cttcaaagaa
     3421 cagtgccagc gcaatcggca aaccgagttg aatcagtcgt ttcataaccg caggatcggg
     3481 cttagcggtg cctttttcgt tacgaatatc gcgcatggag cgggcgcgtt taatgtaaga
     3541 aaccatggca aggaacatga cccaatacac cgccgcagta gccacgccac aaccaacgcc
     3601 accgagctca ggcataccga aatgaccata aataaagata tagttcaccg ggatgttcac
     3661 cagcaggccg ataaagccca ttaccatacc cggcttggtt tttgccagac cttcacattg
     3721 gttacgggca acctggaaga acagatatcc cggcgcgccc cacaacaacg cacgcagata
     3781 acccacggct ttgtccgcca gagccggatc gatgttttcc atggagcgga taatgtaacc
     3841 tgcattccac agcaccagca taatgagaac ggaaacaaaa cctgccagcc agaaaccttg
     3901 tcgcacctga tgcgcaatgc gctcacgtcg accggaacca tttaattgcg cgataaccgg
     3961 cgttaatgcc agcagcagtc cgtgaccaaa gaggatcgcc ggaagccaga tagaagtacc
     4021 gatagcgacc gccgccatgt cggtggcact atagccgccc gccatcacgg tatcgacaaa
     4081 acccatcgca gtttgggcga tttgcgcgag aatcaccggg attgctaatg ctaataacag
     4141 acgcgcttca ctgatatact tctgcacgtg aacacctttt atttgtagtt atatgagaga
     4201 ctaaaaagcc gcctgaatgg gcagcaaaaa gaagaagcag gggaaattca gtctattgta
     4261 gcgaggattt attaattctc cagtgaaaaa atagccattc ttaccccttc actggtaagg
     4321 gcgttttcca cctgctattg tgctgaacag aatgtcttaa ctgatttcag gagttgtaag
     4381 tatgtttacg gggattgtac agggcaccgc aaaactggtg tcgattgacg agaaaccaaa
     4441 ttttcgtacg catgtggtgg agttacccga ccacatgctg gacggcctgg aaaccggtgc
     4501 ttccgtggcg cataacggtt gctgcctgac cgtgacggaa attaacggca accatgtcag
     4561 ttttgacctg atgaaagaaa cgttacgcat taccaatctt ggcgatttaa aagtggggga
     4621 ttgggtaaac gttgagcgtg cggcgaaatt cagtgatgaa attggcggac acttaatgtc
     4681 aggtcatatt atgaccaccg ctgaagtggc gaaaatatta acctcggaaa ataatcgcca
     4741 gatctggttt aaagtccagg atagtcagtt gatgaaatat atcctgtaca aaggatttat
     4801 tggcatcgac ggtattagcc tgaccgtcgg cgaagtcacg ccaacgcgtt tttgcgtcca
     4861 tttaattccg gaaacactgg aacgcacgac tcttgggaag aaaaaacttg gcgcacgcgt
     4921 caacattgaa atcgatccac aaactcaggc agtggtagat acggtagaac gtgtgctggc
     4981 ggcacgagaa aatgccatga atcaaccagg tacagaagcc tgatctgaac tcgcccccga
     5041 agagggggcg atagaatagc ctttagcgag cgactcgaag gccgttttca acaccgcgtg
     5101 agaacaccac ctgccagagc tgaatatcac gggcgcggaa agcacctgcg caggcattca
     5161 gataataggt aaacatccgt ttaaagcgtt cactgtagtt atccgcaatt tctggccatg
     5221 cggcgaggaa tcgttcatac cacgccatca acgtagtatc gtaatcagca ccgaagttat
     5281 gccagtcttc catcacaaag tggggttcgc tggattgagc aatctggcgt acagagggca
     5341 ggcaaccgtt cggaaaaata tatttattaa tccagggatc aacattcaga tcggtttttt
     5401 tcgaaccgat agtatggagc aggaatatac cttccggttt caaattacga tccaccaccg
     5461 caaaataggt atcgtaattt ttcggtccga cgtgctcgaa catccccaca gaaacaatac
     5521 gatcaaactg gtcgttcagg tcacgataat cttgcagcaa aatggtgaca tccaggcctt
     5581 tacagcgttc ctgagccatt ttttgctgtt cggcagaaat ggtgacgccg accacgctta
     5641 cgtcatagtt agatgccatg tagtgcgcca gtccgcccca gccgcagcca atatccagta
     5701 cgcgcatccc tggttttaac tgcaattttt cacaaatcat tttgagcttc gcctgctggg
     5761 cagattccag attatcggca tctttccagt aagcgcagga atattgcatg aagggatcaa
     5821 gcatgcggct gaacaagtca ttacccaaat cgtaatgctc cttgccgact atccaggcac
     5881 gttttttact ctgcagattg aagagacgag cgccggcaat acgcagcgtg tctttgaaat
     5941 gatgggggag ttggttctcg agacctgcgc gtaagacttt gctaaaaaac atatccagtc
     6001 ggtcacattc ccaccagcca tccatataac tttcgcctaa ccccaaagag ccttcctgca
     6061 gaacgcgttt aaaaaaatcg gggtttttca cacgaatatc cgccggggca gaaccgttaa
     6121 tggctatacc ggcacggcta agtaattcgt tggcgatacg gtaccagttg tcatccggta
     6181 cactgacttc ttctatacac gatgaactca tagtttctcc atcactggtc ggtgatcaga
     6241 accttaaaac agcgtagacg ctttttgggc tttgtgagaa atctcgcaga aaaaaccgcg
     6301 agtctataat ccgacgtaga acagagggaa aggaggaacc cttgccgaaa tgaccatcca
     6361 tagtgagtcg ggaacgatcc tgtccccgta aaaacataat atttatcgta gctttaacaa
     6421 ctcgaaatta gtataggccg ctaatttcaa tcatacaaca aaattttgtt agcagcctaa
     6481 gtataagtat atcgatatag atcagtgtga ttcgctatga gcgacttcgg cattgccatg
     6541 attcgggcag tcaacttctt cacaacgttg catcatgtaa cccagcgcga ccagcactac
     6601 tgttgataac atcacgctgg tggtggtgag caatggcgtg ctgatactaa taagccagga
     6661 aactaccaga cttgcgagga agcacagacc cagttgcaga gtgttctgca acgctgcggc
     6721 gcgaccggtt gcatgtggga agggacgcag cgcctgggcg acaacaatag ggtagatcgc
     6781 gccattggca atcgccatca cacagaatgg gatcaggatt tcgaccagcg acacatggct
     6841 aatgaagccc gcagcccagg tcgcaatgac gctgacagca aacagcacca gcaaccacgg
     6901 taataactgc ttgccttgcc atttctgcag tgcggtgcga cagccataac caccaatcag
     6961 aaacgcgata gtttgcggga cataacttaa accaataact gccgggctgt agcccatttc
     7021 actaaggatg aacggtgaac cggtcagcca tgcaaaaaaa ctggctgaac aggctgcgta
     7081 tatcagcacg ttgccgcgat aggttttaga acgtagcagg tcggtaaagg tcagaccatc
     7141 ctgactattg ttacgggcct tcgtcgtggg tttgagccag aaaataggca gaatcagcac
     7201 cacggtaatg gcaaacaggg tggcgaaaat cgcctgccag gaaaaatgga ccagcagcca
     7261 gcttcctaac aggggagcca gtgccggaga cagacccacc agcggcatga tggtcgcaaa
     7321 aatacggttg actttctgtg aaggataata atctgtcact aatgcttgcc agataaccgc
     7381 cgcggcgcag acccccacag cctgtacaaa acgcaatacc agcagcgtgg cggcgttttc
     7441 tacccacagc atccccagac tacctaacgc aaaaattgtc aggccgatta ataacaccgg
     7501 tttacgacca taacggtcgg agagtggccc ccacagaagc tgggctgcgg caaaaccggc
     7561 aaggaacaga ctaaggctgg cactgacagc agacgcaggc gtttgcaggt cggcctgtat
     7621 ggcggcgaaa gcaggcagat acatatcggt tgccagaaaa ccgagtacgc tcaaacccgc
     7681 cagccagact aaaaatcttt tcccaggttg catgtatatt ctctaaatct ctcaattaaa
     7741 caccgccgca aagtgtaggg agtgaaatta gtgttgtgaa acggtaatat ttgatgactg
     7801 gtttcaaaaa ttttgcaggc tgactatgtg gtcagaatat tcactcgaag ttgttgatgc
     7861 ggtagcgcgt aatggtagtt ttagcgctgc ggcacaggag ctgcatcgcg ttccttctgc
     7921 ggtcagctat accgtgcgtc agctggaaga gtggctggcg gtaccgctct ttgaacgacg
     7981 gcaccgtgat gtggagctga ccgctgctgg agcgtggttt ctcaaagaag ggcgctctgt
     8041 tgtcaaaaaa atgcagatca cccgccagca atgtcagcag atagcgaacg gctggcgcgg
     8101 tcagttagct atcgcagtgg ataatattgt caggccagaa cgtacacggc agatgatcgt
     8161 tgatttttat cgccattttg atgatgttga acttcttgtc tttcaggaag tgttcaacgg
     8221 tgtctgggat gcgctttccg acgggcgcgt ggaactggca attggcgcaa cacgcgcgat
     8281 tccggtaggc ggtcgttatg ccttccggga tatggggatg ctaagctgga gttgcgttgt
     8341 tgccagtcac cacccgctgg cgttgatgga tggcccgttc agcgatgata cgttgcgcaa
     8401 ctggccgtcg ttggtgcgcg aagacacctc gcgaacgcta cctaaacgta ttacctggtt
     8461 gctggataac caaaaaagac ttgtcgtacc tgactgggaa tcatcggcaa cctgtatctc
     8521 ggcaggatta tgcataggga tggtgccaac acatttcgcc aaaccgtggc ttaatgaggg
     8581 gaagtgggta gcacttgaac tggaaaaccc cttcccggat tcggcatgtt gtctgacatg
     8641 gcagcaaaat gatatgtcgc ctgcgttaac ctggctgctt gagtatctcg gcgatagtga
     8701 gacgctgaat aaggagtggc tgcgggagcc ggaagagact cccgcaacgg gtgattaacg
     8761 acgatagtcg cggaacgggc cgtcagccac ggagcggcgt tcaatcaaac gcggatgcac
     8821 ttcaatggac tgcggttctt cacgtttgtt gacgatacga tccaacagca tgttgaacgc
     8881 tgtttcaccc agcgaatctt ttggctgatg gatcgtggtc agcgccggcg taaaatagcg
     8941 cgcgttgcgc acgttatcat aaccgatcag cgaaacatcc tgcgggacgc gcaggcccat
     9001 ttcatcagca gcacaaagtg cgcccattgc catgatatcg ccaccacaga agacggcagt
     9061 agggcgatgc ggctgcgaca ggatttgctg catggcgcga taaccggatt caggttcaaa
     9121 gtcaccctgc acaatccagc tttccggcac cttgatcatc gcttcttcca tcgccttcat
     9181 aaaaccggca aggcggcctg cgccggtgtt acgttccagc gggccgggga tgacgccgat
     9241 ttcgcggtga ccgcgttcaa tcagataacg cccggccatg tagccgcctt cgaacgcgtt
     9301 atcaatgacc gcatcggtga agtcagcttt tgcttcaccc cagtccatca ccaccattgg
     9361 gatatggcga tactcttcca gcatcgccag caacggctct gggtactcag aacacatcac
     9421 cagcagacca tcgacgcgtt tttgcgccat catcgacaga taagcccgct gtttctcaag
     9481 attgttccac gcattaccca gaatcagggt gtaacctttc tggaagcaat ttttttcaac
     9541 tgcttcaatg atctcggcaa aataggccgc ttcgctgctg gtcgccagca aaccgataga
     9601 cttggtgtgg ttaaccttca ggctacgcgc caccgcgcta ggggagtagt gtaattcttt
     9661 aatcgctgcc cacacggcgt tgcgcgtttc ttcagcgacg aaacgtgttt tgttgatcac
     9721 gtgtgacaca gttgtagtgg aaacgtttgc tcgtttcgct acatctttta ttgttgccat
     9781 tccatttcac tccagaccct aacttcagct cctgcaaaat cgcaaggtaa acgtttgcct
     9841 ttacacacct tacgcacaac aaaagtgttg cggtacgccg gaaaaacgac acggagcgtc
     9901 aggaaggggt caatggccag tacgcaaata gtgtaagcgt ggaattttgg ccgatcttga
     9961 cgaaaagggg aagtggtaaa aacacttatc attataaatg acgggagatt ttttcataac
    10021 agtgtgtaaa aatgcgtaat atcacttttt gtgtggtaat aaaaaaggag aaaacttgat
    10081 gagcaccgac cttaaatttt cactggtaac aacgattatc gtcctcggtt tgatcgtagc
    10141 cgtgggtttg actgccgcgc tgcactgatt tcctctcgag ggagcgcgtt ctccctcgca
    10201 tctccttcct gtttgttagc aaaatggcaa aagtgttttc tctttttgtt attttcattt
    10261 ttttgtgatt gctgtcatgc tttcgccact ttaatgaata tcgcgtaatg acgcgaagag
    10321 gtggagataa ggcatgaaaa ttaactatcc gttgctggcg ctggcgattg gcgcgtttgg
    10381 tatcgggaca acggagttct cgccaatggg cttgttgccc gtcattgcgc gcggtgtgga
    10441 tgtctcgatt cccgctgccg gaatgttaat cagtgcctat gcagttggcg taatggtggg
    10501 cgcgccgctg atgacgcttc tactttctca tcgtgcccgc cgcagtgcgt tgattttcct
    10561 gatggcaatt ttcacgctag gcaacgttct ttccgccatc gcgccggatt atatgaccct
    10621 gatgctttca cgtattttga ccagcctgaa tcacggagca ttttttggtt tgggttcagt
    10681 cgtggccgca agcgtggtgc caaaacataa acaggccagc gcagttgcca ctatgtttat
    10741 ggggttaacc ctggcaaata tcggtggcgt gccggcggcg acctggttgg gtgaaaccat
    10801 cggctggcgg atgtcatttc tggcaacggc ggggctggga gtgatttcaa tggtaagtct
    10861 gttcttctca ttacctaaag gtggtacagg ggcacgacct gaagtgaaaa aagagctggc
    10921 ggtattaatg cgtccgcagg tgctgtctgc attgctgacg acggtactgg gagctggtgc
    10981 aatgtttacc ctctacacct atatctctcc ggtactgcaa agtattaccc acgcaacacc
    11041 ggtgttcgtc acggcaatgc tggtgctgat tggtgtcgga ttctctatcg gtaactatct
    11101 cggcggcaaa ctggcagatc gttcagttaa cggcacgttg aaaggctttt tgttgctgct
    11161 gatggtgatt atgctggcaa tcccgttcct ggcccgcaat gagttcggcg cagctattag
    11221 catggtggtg tggggcgcag caacctttgc ggttgtacct ccgttacaga tgcgcgtgat
    11281 gcatgtcgcc agtgaagcgc caggtctgtc ttcatcagtc aatattggtg cctttaatct
    11341 tggaaatgcg ctgggagcag ctgctggtgg tgcggtaatt tccgctgggc tgggatacag
    11401 ctttgtgccg gtgacggggg cgattgtcgc gggactggca ttattgctgg tgtttatgtc
    11461 agccagaaaa caacctgaaa cagtttgcgt tgctaacagc taattaaaca aaaacgcaga
    11521 aggataacct tctgcgttac agttgctgac aacgtgcgat ttatcttgcc gaatgcgctg
    11581 ttaacgcctc atccgcgttg gcatggtaag cgaagcgcat caggcaatgc tgcatttgcc
    11641 atcagttatt atgcagcgag atttttcgct acgaattccc agttcaccag cgcccagaag
    11701 tgctccagat agccaggacg tgcattgcga tagtcgatgt aataagcgtg ttcccagaca
    11761 tcaacggtca gcagcggagt cgcatcggtg gtcagcggag tacccgcgtt agaggttgaa
    11821 acgatagcca gtttgccatc gctgtttttc accagccagg tccagccaga accaaagttt
    11881 ttgatcgctg catcagtaaa ctgcgctttg aaatcggcaa agctgccaaa agatgcggcg
    11941 atagcttcag cgacttttcc agtcggttcg ccaccggcgt tcggtgccag gcagttccag
    12001 tagaaagtat ggttccagac ctgagctgcg ttgttgaata cgccaccttc agagctgcga
    12061 ataatctctt ccagtgattt accttcaaac gcggtacctt taatcaggtt gttcaggtta
    12121 gtgacataag tctgatgatg cttgccgtag tgatactcga tagtttccgc agaaatgtgc
    12181 ggtgccagag catctttagc atatggtagt gcaggtaatt cgaatgacat tgctactctc
    12241 ctttattatt aatttgcata cgtacaatag ccttattgtg cgtatggata gggtagcaaa
    12301 ttaaaaggtg agacaaaaga tgaacttacc ctgttgccgt aacaacaggg taaagtttta
    12361 gcgaagtgtt tttggggtca ttacccgacg agcgccaaca tagtggcgct gccagtagtc
    12421 ttcactgaga gaagtgattt gaatttcctg acctgtgcgc ggtgactgaa taaatttgcc
    12481 gttgccgaca tacacgccga catgatcggc tgtaccgcgt ccctgagtac ggaaaaagac
    12541 caggtcgccg tttttcagct cactacgttc gataggtcct gcatcacgca ggtgatacat
    12601 ttcattcgcc gtacgcggaa tacgaatttt caccaaatct ttataagcgt aataaaccag
    12661 gccgctgcaa tcaaaaccgg tacgcggtga gctgccgccc caacgatatg gcttaccaat
    12721 ttgctgcatc agtttattca ttgccacttt cgtggctttt tgcaccttcg ctttgtgcgc
    12781 gtcggcaagg gttcctgacg cggcattttt gactttcaca caatgcgatt tatagccctt
    12841 acgtttggta cacttttcgg taaagctggc tgaggccgtt ttattgacgg cgtgtttgct
    12901 gcgagttttg gcgtttttag aagcggtaat ggaagatttt ttaacggaag aagcggtttt
    12961 gcttttggtc gtacttttac tggcggcttt tttcgctgtt ttctggctgg ctttctgggt
    13021 ctttttggtg gttgccgtgc ttttcttttt atctgctttt ttggtgatat gagcggtagc
    13081 agaactctcc ctggcttgct ttgaagcatg ggccataggc gttaaaggca gggtggtaaa
    13141 aagtaaagca cagagcgtga tcgaaatacg gtttatccgc gccactgagc gtccctctgt
    13201 taaggcaggt ctatcatgcc tgcgtctgat ttacaaaact tgatgattct aatgcataga
    13261 agaagagaaa gttaatagct tttattgtta aaaaatacgt ttcgttggcg agcgcaaaaa
    13321 aatgcagcag tgaaagctaa aaatggttat aaatacgcca gtttgccgcg catcagagaa
    13381 gaaacgaata tttgaaatgc aacaaataga gcaaagggat aaaatagcaa aagcgtgatg
    13441 aaaatgttat tttccgatgc cgcactgacc cgctacaatg tcagattact gccgtaaaga
    13501 agtttgagga agcaagacaa tgagcaccac tatcgaaaaa atccaacgcc agattgctga
    13561 aaacccgatc ctgctgtaca tgaaaggttc accgaaactg ccgagctgcg gtttctctgc
    13621 ccaggcagtc caggcgcttg ccgcatgtgg cgaacgtttt gcctatgttg atattctgca
    13681 gaatccggac attcgtgcgg aactgccgaa atatgctaac tggccgacct tcccgcaact
    13741 gtgggttgac ggcgagctgg tcggcggttg tgatatcgtg atcgaaatgt atcagcgtgg
    13801 cgaactgcag cagctgatca aagaaactgc cgctaaatac aagtctgaag agccggacgc
    13861 ggaataatcc gtcctgcaat actgacggat gcgaacgggc atccgtcagt cctttatccg
    13921 gtgcgccgta aaacctcgtc cttcagggcg gggatataag gcgcggtttt ccacctgacc
    13981 aggtgtttgc tgttgttcaa tgtattggcg gatgacggat attggcgcac cttcgcagct
    14041 actggcaaaa tagccaggac tccacaaaac gcctttgtag taatacctga ctgcaatatc
    14101 tggtcgatct cgtcgcagta acctattcga tatgcctttg aggctgttta ccagactgga
    14161 tatcgccagt ttgggaggat agttaattaa caaatggacg tgatctggtt cgccatccat
    14221 ttcaaccagt tcagctgcaa aatcagcaca tacatttgaa aagtaagtgc gtagtttttc
    14281 tgtcgtgtca tggtcaaaaa tctggcgtcg gtatctggtg acaaagacca ggtgaacatg
    14341 catcaggaaa acacaatgcc ttccacgccg gatatcggtt tcttttttca cagaccaaag
    14401 tatcatttga cctgtgaaac gattacaggc atttaaattc cagttaagac ccggtggtca
    14461 acaggagcgt gaaatgaggc gcttcgcagg cgcatgtcgt ttcgttttca atcgtgctct
    14521 ggcgcttcag aatgagaatc atgaggccgg aaataaatac atcccttacg ggaaaatggc
    14581 ttcctggctg gttgagtgga aaaacgccac tgaaacgcaa tggcttaaag attcgccctc
    14641 acagccattg caacagtcac tgaaagacct tgagcgggct tacaaaaact tcttccggaa
    14701 gcgggctgct tttccccgat tcaaaaagcg gggacagaat gctgcattcc gctacccgca
    14761 gggcgttaag ctcgatcagg aaaacagccg tatttttctg ccgaaacttg gctggatgcg
    14821 ctaccggaac agccgtcagg tcacgggagt tgtgaaaaat gtcactgtca gccagtcctg
    14881 cggtaagtgg tacatcagta ttcaggcaga aagtgaagta tcaactccgg ttcacccttc
    14941 agcatcaatg gtcgggctgg atgctggcgt ggctaaactt gcctcgctgt cagatggcac
    15001 agtctttgag cctgtaaaca gttttcaaaa aaaccagaag acgctggcga gacttcagcg
    15061 acagttaagc cggaaggtca aattcagcaa caactggcag aagcagaaac gcaaaataca
    15121 gcgactgcat tcctgtatcg caaatatccg cagggactac cttcacaaag tcacaacgac
    15181 cgtcagc
//
LOCUS       DAJTNM010000092        14933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTNM010000092 DAJTNM010000000
VERSION     DAJTNM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.092"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            86..367
                     /locus_tag="OO172_002941"
     CDS             86..367
                     /locus_tag="OO172_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513056.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVIAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHGKKPERLRR"
     gene            complement(1164..2624)
                     /gene="phoQ"
                     /locus_tag="OO172_002942"
     CDS             complement(1164..2624)
                     /gene="phoQ"
                     /locus_tag="OO172_002942"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5513057.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2624..3295)
                     /gene="phoP"
                     /locus_tag="OO172_002943"
     CDS             complement(2624..3295)
                     /gene="phoP"
                     /locus_tag="OO172_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5513058.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3464..4834)
                     /gene="purB"
                     /locus_tag="OO172_002944"
     CDS             complement(3464..4834)
                     /gene="purB"
                     /locus_tag="OO172_002944"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5513059.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4838..5479)
                     /gene="hflD"
                     /locus_tag="OO172_002945"
     CDS             complement(4838..5479)
                     /gene="hflD"
                     /locus_tag="OO172_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5513060.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5515..6621)
                     /gene="mnmA"
                     /locus_tag="OO172_002946"
     CDS             complement(5515..6621)
                     /gene="mnmA"
                     /locus_tag="OO172_002946"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5513061.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGHEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6675..7136)
                     /gene="nudJ"
                     /locus_tag="OO172_002947"
     CDS             complement(6675..7136)
                     /gene="nudJ"
                     /locus_tag="OO172_002947"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5513062.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7146..7799)
                     /gene="rluE"
                     /locus_tag="OO172_002948"
     CDS             complement(7146..7799)
                     /gene="rluE"
                     /locus_tag="OO172_002948"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5513063.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7971..9221
                     /gene="icd"
                     /locus_tag="OO172_002949"
     CDS             7971..9221
                     /gene="icd"
                     /locus_tag="OO172_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5513064.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9324..9647)
                     /gene="iraM"
                     /locus_tag="OO172_002950"
     CDS             complement(9324..9647)
                     /gene="iraM"
                     /locus_tag="OO172_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5513065.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(9900..9944)
                     /locus_tag="OO172_002951"
     CDS             complement(9900..9944)
                     /locus_tag="OO172_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513066.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            complement(10180..10290)
                     /locus_tag="OO172_002952"
     CDS             complement(10180..10290)
                     /locus_tag="OO172_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513067.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(10343..10747)
                     /locus_tag="OO172_002953"
     CDS             complement(10343..10747)
                     /locus_tag="OO172_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR5513068.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10968..11699)
                     /gene="bluR"
                     /locus_tag="OO172_002954"
     CDS             complement(10968..11699)
                     /gene="bluR"
                     /locus_tag="OO172_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCR5513069.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11904..13115)
                     /gene="bluF"
                     /locus_tag="OO172_002955"
     CDS             complement(11904..13115)
                     /gene="bluF"
                     /locus_tag="OO172_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCR5513070.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            13429..13665
                     /locus_tag="OO172_002956"
     CDS             13429..13665
                     /locus_tag="OO172_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCR5513071.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13708..13980
                     /locus_tag="OO172_002957"
     CDS             13708..13980
                     /locus_tag="OO172_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCR5513072.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            14009..14275
                     /gene="ariR"
                     /locus_tag="OO172_002958"
     CDS             14009..14275
                     /gene="ariR"
                     /locus_tag="OO172_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCR5513073.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            14388..14636
                     /locus_tag="OO172_002959"
     CDS             14388..14636
                     /locus_tag="OO172_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513074.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         4007 a         3401 c         3480 g         4045 t
ORIGIN      
        1 gagcatgagg attttgcttt tgatataagc gacagccacc gccagaaact ggaagaattt
       61 agcggggtgt aatgaggtca gcagcatgac agaagccgaa ataacgggat taatccgccg
      121 cgttgccgga atcagccagc aggctgacga acaggccacg cagccggaca gcgtgatagc
      181 cgaaaactat gcgcgtgttg ttgctgaggt gatgcgccgt gatggtatag aacttaatgg
      241 cgtggatatg cgcaacatac gaaccagagt tcttgagttg ctggcctacc gtcgccgcgt
      301 ggagatgtat cgggagaagg agaaaatcac gtaccacggg aagaagccgg agcggttacg
      361 gcggtaactc actgagatta aagcaaagcg caaaattgcg ctggctacat ccctcacatc
      421 tgaggactgt taaccagcag gccggaatat attcagaaac tacgaaattt tcgcagttta
      481 attactcgtc agcttaatcg ctaacccgct ctgatatttt cggaaacctc aatttgagga
      541 agtcggcgag gtaactgttt gatattcccg atagcgcaaa aatgcgcggt cgggcagaat
      601 gaccaccacc tcaatcgtga cggagagtga caaccagtga cacaccgtga caaaaagacg
      661 gtttaaccgt tcattaaaaa tcgacgtgat tttgtgcttt ccggatgacg tggccaacgt
      721 cattttttag cgaaaaattc tgattaaggt cactggctga acggtcataa tgaccacgca
      781 gaccatcgag gcaaccatag cgatcatcgt tacggttgga gcttttccgc cattggtgga
      841 aaaggtgcac atctggatca tcattacggt gatcaagggt atgtaaaacc caccagcctg
      901 attaaaggtt aaccggaaaa aaaatccggt agccccgtta tggggatccc catatcgaca
      961 ttaacgcccc tcatgaattg gtgcgcttcc ccctggaaag attacccgcc tgtatatttc
     1021 ttgtgtctat ttgttccacg ttgtttcata cagtgcaccg aacggtgtag ttaatggtgt
     1081 agtcaatttg cgatttttgg cactttttca atgaaaattg tttaatcgaa tcaatgactt
     1141 gatgtagtgg taaaaggaca tatttattca tctttcggcg cagaatgctg gcgaccaaaa
     1201 atcacctcca tccgcgcacc gcccagcatg ctctctccgg cgacgatttt accctcatat
     1261 tgctcggtga tttcgcgggc taccgccagc cctacacctt gcccagggcg taaagtatca
     1321 acccgttgac cacggtcgaa aatgacctct cgcttgctta atggaatacc ggggccatca
     1381 tcctcgacca caatatagag atgctcgtcg gtttgccttg cagaaatttc gacaaactcg
     1441 aggcaatatt tacaggcatt atccagcacg ttgcccatca cctcgacaaa atcgttctgc
     1501 tcaccgacaa agctgatctc tggcgaaata tcgagagaga tattgacccc tttgcgttga
     1561 tacactttgt tcagcgctga ggtgagattg tccagcagtg gggcgaccgg atgcagctcg
     1621 cggctgagca atgtcccgcc gcgcatactg gcacgatgca ggtagtagcc aatttgctgt
     1681 gaaatgcggc tgatttgctc cagcattacc ggctcagcat cactgacgct catcttttca
     1741 ctacgcagag aacgcagcgt actttgcagc accgccagtg gcgttttcag actatgggtc
     1801 aggtcggtga gcgtcgtacg gtatttgtcg taacgttcgc gttcactttt taacaatcgg
     1861 ttcaggtttc gtaccagact ggtcagttct cgcgttgtgg ctggattgag caattcgcgg
     1921 ttatgttctt ccagttcgcg gacttctttt gccagggctt cgatggggcg taaactccac
     1981 caggcggcga cccacagcag cgggatcact aacagcagat tggctgagag tacatagata
     2041 aaccagctcc agaccatata ggaacttttt agctccaccg gaatggtatc caccaccaca
     2101 atggttaatt ttggcatccg cgatgttgcc gggtagacgt ttaccgccac cgagtgggtc
     2161 atctccgcgt cgtcatcatc ttcccgcact tcctgcaact gttgctgtat cgaatgatct
     2221 ccactcagca agaggctggt atcgttaaca tccgcttcaa tttcatgaaa accattcgat
     2281 ttcagccagt caggctggat cattttcatc agccagggca cgtcacgttg cgcccataaa
     2341 agctgcccgt tctcatcata aattagcgtc atggtggggc tttgcttgtc gatattttcg
     2401 ggtaactcga catgcaactt attgttttcc cactgcgcaa gggtatagaa cagattgctc
     2461 tcgccacgta acagccgaaa cgtagtttta tcgaaactga cgctataacc gatcagcgcg
     2521 accattccgt aggcaagcga aagcaccagt actaccgctg ccgttgccaa cagaaaacgt
     2581 acccgcagcg agagcgggaa aaaaagacgc agtaattttt tcatcagcgc aattcgaaca
     2641 ggtagccctg gccgcgaacg gtggtaatga cttcttgcgg atattgtgcc tgaatttttt
     2701 tgcgcagacg tcccatcagt acatcaatgg tatggctttc ccgcagctcc gcatccggat
     2761 agagttgtag cattaacgaa tctttgctga ccactttgcc attattgcgt atcaacgttt
     2821 ccataatggt gtattcgaac gcggtcagtt tgatcacttc gtcattaata gataattcac
     2881 ggcgagagag atcaacctga aacgggggga gcgaaatgac ctgtgaagcc agaccgctat
     2941 tacgccgcat taatgcctgc attcgcgcca tcacctcttc aatatgaaac ggtttagtca
     3001 cgtaatcatc agcaccggca cttaatactt cgactttgtc ctgccagctt tcacgggcgg
     3061 ttaataccag aatcggcagt gaaacatcgt tgctacgcca gcggcgaatc agtgacagac
     3121 cgtcctcgtc aggcaatccg agatcgacaa tcgcaatatc cggtaaatgt tcattgagat
     3181 aataatcggc ttctttggca tcttctgcgt catcgacctg atgaccagca tcctgaatct
     3241 gaactttaag gtggtgacgt aacaacgcat tgtcttcaac aaccagtacg cgcattttta
     3301 tttctccctg tcttaattat taaaatagtg taacgcgatt atgtgtttat gggggtaaac
     3361 attaaataaa ccagcgggga ggggaggtaa agtgaaaaaa gaaaaagtgg ataatcttaa
     3421 taagcaggcc ggacagcatc gccatccggc actgatttga ggcttatttc agctcatcaa
     3481 ccatcgtgat agctcgacca atatagttag ccggcgtcat cgctttcagg cgggctttct
     3541 cttcttctgg caacgccaga ccatcgataa actgcttcat gccttcggcg tcaacgcgct
     3601 taccgcgagt aagctctttc agcttctcgt acggtttttc gatgccatag cgacgcataa
     3661 ctgtctggat tggttctgcc agcacttccc agttgtgatc cagctcatcc agcagatggt
     3721 cacggttcac ttccagtttg ctcacgcctt tcagggtgga ttgatacgca atcagcgcat
     3781 aaccgatacc cacgccgagg ttacgcagca cggtggagtc ggtcaggtca cgctgccagc
     3841 gggaaaccgg cagtttgctt gccagatgct gcaataccgc gttggaaagg cccagattcc
     3901 cttcggagtt ttcgaagtcg atcgggttaa ctttatgcgg catggtggaa gaaccaatct
     3961 caccagcaat ggttttctgt ttgaagtggt taagggcgat ataaccccag acgtcacggt
     4021 caaagtcgat cagaatggtg ttgaagcgcg caacgcaatc aaacagttcg gcaatgtagt
     4081 cgtgcggttc aatctgggta gtgtacgggt tccattgaat acccagcgag gtgacgaact
     4141 cttcgctgaa ctgatgccag tcaacttccg ggtaagcggc gatgtgggcg ttatagttac
     4201 cgaccgcgcc gttgattttg ccgaggatct ccacctggtt aagctggcgg tgctggcgct
     4261 ccatacggta ggcgacgttt gccatctctt taccgatggt tgacggcgtg gctggctgac
     4321 cgtgggtacg agacagcagc gggatatcgc ggtactgaac ggcgagatct ttaatgccat
     4381 caatcagttg acgccagtac ggcaggatca cttcatcacg cgcggttttc agcattaatg
     4441 cgtgggagag gttattgata tcttccgaag tacaggcaaa gtggatgaat tcagaaaccg
     4501 cgtgcagttc cgggatctcc gccacttttt ctttcaggaa atactcaacc gctttaacgt
     4561 cgtggttagt ggtacgctcg atggttttga tgcgtgcggc atcttcttcg ctgaaattgg
     4621 cgacaattgc atcaaggtaa ccgattgcgt cggcagcaaa agcaggaact tccttgatcg
     4681 ctgcgtgcgc ggccagtttt tgcagccaac gtacttcaac ttgtacacgg aatttcagca
     4741 aaccatattc gctgaaaatc ccgcgcagcg cgctgagttt atcgccgtag cgtccatcga
     4801 caggggaaac ggcggtcagt gaggataatt ccatagatca caactccggg gttaaatgag
     4861 caagaatttg ttttgcctga gtggtcaggc gattacgaga aaacatcagt tgtagacgtc
     4921 cgccgccgac ctggtgccag agcacggcgg cgcgaatgcc tgccagcagg gttgcgcgaa
     4981 ctttcgcctg cacttgtggg ctttgcagta cagcagggga accggtgacc tgaatgcgcg
     5041 ggccaagcgg gctgatcaca tcaacataga tagcagccat cgcgctcatc agcgtttcgg
     5101 actgtaaatc gaagtgttcg agctggcgtt gcaggccgtt gatccggttg cccagagtgt
     5161 cgagcgcgcc tttcgctgag gagagtttgc gctcaagcac catcaagctg agtgtgtagc
     5221 gggttaattc ggcgtttaag ccctggcaac tgctggcatt gagcacgccg agcagggttt
     5281 ccagcccgac gcgcaggttg gcttcgctac cgccaaaaac cgccagcgtc gagctggggt
     5341 tcatatcaat aatactgttg agtgagacgt gtagcgcatc ggcatcacaa tgcccctggt
     5401 gagcgagttg ttgcaccagg cgtgccgact gacaaatacc ggccagggcg agggtgatgt
     5461 catagtaatt ctttgccacg ttcactgctt ccttgtttta agtaaagata ataatcagac
     5521 cggcagcggc agacgctgct caataatacc gccaccgagg cacacttcac cgttatagaa
     5581 gacggcagac tggcccggcg tcacggcggc aaccggctca tcgaaaatca ctttaatgcg
     5641 atcgtcgtcc agcgccttga cggtgcacgg aatgtcggtc tggcgatagc gggtttttac
     5701 cgtgcaacgc atagtgccgg tgaatggttc gcgatcgacc cagtgcagct gctgggcaat
     5761 caacccgaca gacatcagcc gcgggtgttc atggccctga gcgacaacca gaatgttgtt
     5821 ttcgacgtct ttgtccacca cataccacgg ttcttcggtg ccttctttgg tgccaccgat
     5881 acccagacct ttacgctgac cgagagtgtg atacatcagc ccctggtgct caccaatttc
     5941 atggccatcg acggtaatga ttttgcccgg ttgcgccggg agataacggc ccaggaactc
     6001 gcggaattta cgttcgccaa tgaagcaaat gccggtagag tcttttttct tcgcggtgac
     6061 cagaccaaga tcttcagcaa tcttacgcac ctgcggtttt tccagttcgc cgaccgggaa
     6121 caggctttgc gcaatctgct catggctgag cgtataaagg aagtagctct ggtctttatt
     6181 gctgtccaga ccacgcagca ggcggctctt gccatcgaca tcggcccgac gtacgtaatg
     6241 accggtagcg atataatcgg cacctaaatc ttcggcggca aattcgagga aggctttaaa
     6301 tttgatctcc ttgttgcaca gaatatccgg attcggcgtg cgaccggctt tatattcggc
     6361 aaggaacagt tcgaagacgt tgtcccagta ctcggcagca aagttaacgg tgtgcagttc
     6421 aatgccgagc ttgtcgcaga ccgcctgggc atcagccaga tccgctgccg ctgtgcaata
     6481 ttcctcaccg tcgtcttctt cccagttctt cataaacagg ccttcgacct gatatccctg
     6541 ttgttgcaac agccaagcag aaacggagga atcgacaccg ccggacatgc cgacgattac
     6601 tttttttgcg gtttcagaca ttggatcact cacgacattg aacttcaagg cggcgtattc
     6661 tatcacgcag ccttttagat gacacccttt gtaaaaggcc agttaaaatc gccaatcatc
     6721 tccagcggat aacgttgccc gctttgataa caacgaatac tttccgccac cagcggcgaa
     6781 cgaagatttg acgcctgtaa aatttcttcg gcgctgaccc aacggcagca gtcgatatcg
     6841 ctgtcatgag gctgggtcgg gcatatttgc tcaagctcaa tggcaaagag gaaacgcaaa
     6901 aacggcgttt tatctggcgc aatccactga tgcatacgaa taaagtgttg cggctgcgcg
     6961 ctgatgccgg tttcttccca cagttcacgg gcggcggctt ccactaaggt ttcatcggct
     7021 tccagatgcc cggcaggttg gttccataac gctttaccat taatcgtctc ttcaacgact
     7081 aaaaatttgc cttctgcgtg caccacgcaa gcaacggtaa cgtgcggttt aaacattttg
     7141 catccttaat ctgtcacttc tcgccattcg ccattggcaa gattatccaa agagtaatct
     7201 cccatcgcat agcgaatcag tcgcagcgtg gggaaaccaa catgggcggt catgcggcgc
     7261 acctggcgat tacgtccttc atataaggtg atcttcagcc agctggtggg aatacttttg
     7321 cgttcacgaa ttggtggatt ccgcggccat aaccacgcgg gttcgtcaac cagctccgcg
     7381 cctgcgggca gggtagggcc atcatttaag gttacgccat tgcgcaaggc ttcaagtgcg
     7441 tcttgtgtgg gaataccttc cacctgcaca taatagattt ttccggtgcg tttacccggc
     7501 tgggttaaac gcgcctgcaa cgcgccgtta ttggtcagca ccagcaaccc ttcgctatcg
     7561 cggtcaaggc gacctgctgc ataaacgccc tgaaccggga tgaattcttt taatgttttg
     7621 cgtccggctt catcggtgaa ctgcggaaga acatcgtagg gtttattgaa gaggatcaca
     7681 cgcgtgggct ggttttcatg tttacgcctg gtagaacgtt gcgagctgaa tcgcttaacc
     7741 tggtgatttc taaaagaagt tttttgcatg gtattttcag agattatgaa ttgccgcatt
     7801 atagcctaat aacgcgcatc tttcatgacg gcaaacaata gggtagtatt gacaagtcaa
     7861 ttacaaatca ttaacaaaaa attgctctaa agcatccgta tcgcaggacg caaacgcata
     7921 tgcaacgtgg tggcagacga gcaaaccagt agcgctcgaa ggagaggtga atggaaagta
     7981 aagtagttgt tccggcacaa ggcaagaaga tcaccctgca aaacggcaaa ctcaacgttc
     8041 ctgaaaatcc gattatccct tacattgaag gtgatggaat cggtgtagat gtaaccccag
     8101 ccatgctgaa agtggtcgac gctgcagtcg agaaagccta taaaggcgag cgtaaaatct
     8161 cctggatgga aatttacacc ggtgaaaaat ccacacaggt ttatggtcag gatgtctggc
     8221 tgcctgctga aacccttgat ctgattcgtg aatatcgagt tgccattaaa ggcccgctga
     8281 ccactccggt tggtggcggt attcgttctc tcaacgttgc cctgcgtcag gaactggatc
     8341 tctacatctg cctgcgtccg gtacgttact atcagggcac tccaagcccg gttaaacacc
     8401 ctgaactgac cgatatggtt atcttccgtg aaaactcgga agacatttat gcgggtatcg
     8461 aatggaaagc tgactctgcc gacgccgaga aagtgattaa attcctgcgt gaagagatgg
     8521 gcgtgaagaa aattcgcttc ccggaacatt gcggtatcgg tattaagccg tgttctgaag
     8581 aaggcaccaa acgtctggtt cgtgcagcga tcgaatacgc aattgctaac gatcgtgact
     8641 ctgtgaccct ggtgcacaaa ggcaacatca tgaagttcac cgaaggcgcg tttaaagact
     8701 ggggctacca gctggcgcgt gaagaatttg gcggtgaact gatcgacggc ggcccgtggc
     8761 tgaaagttaa aaacccgaat accggcaaag agatcgtcat aaaagacgtg attgctgatg
     8821 cattcctgca acagatcctg ctgcgtccgg ctgaatatga tgttatcgcc tgtatgaacc
     8881 tgaacggtga ctacatttct gacgctctgg cagcgcaggt tggcggtatc ggtatcgccc
     8941 ctggagcaaa catcggtgac gaatgcgccc tgtttgaagc cacccacggt actgcgccga
     9001 aatacgccgg tcaggacaaa gtaaaccctg gctctattat tctctccgct gagatgatgt
     9061 tacgccatat gggctggact gaagccgcag acctgattgt taaaggtatg gaaggcgcga
     9121 tcaacgccaa gaccgtaacc tatgacttcg agcgtctgat ggaaggcgct aaactgctga
     9181 aatgttcaga gtttggtgat gcgatcatca agaatatgta atcactacat gtgttaaata
     9241 tttaaacggg cgtataacac gcccgttgtt ttatttatgt ggatattatt aatagcatat
     9301 cgagcatatt tatataaaac ctattacttg agcccatatg ggcatatttt tataatacaa
     9361 ctcttatgca aacatttatt tgttatttcg ctttctcctg gaggacactc ttgactgctt
     9421 ttgagtaaac tccataaatt cttgttgaat ggtgcaatgt tataaatagt aataggatat
     9481 tctttttcct taaggataat accaagctta accggtgtaa atatccttcc aggaggaaga
     9541 aatatagtag attgatacca gatgatcatt ttcatatcac cccatatggc tgaaaaagat
     9601 atgccgcatg taggttgaat taccgtgtca attactaccc acttcatttg ttatgtctta
     9661 tcccacggta tttaatatag ttcatttgga tgttcatttc tttattttgc atatgagtat
     9721 attacccctt caaaaaataa attaattaaa acgattgctt atataaaaca aaatttaaat
     9781 caaggaatct caatggatgt taaacaaaat gagatttagt gaaaacaata aattattcac
     9841 ttcgttttag atttgtttag ctataatgtt atacattcaa atgactgaac atcctgtaat
     9901 taaaacatag cctttatgct acttggtgcc aatttgctaa acattatggt tgccttttta
     9961 tataacgata ataatgaata taaacatgac atgagaataa ggtttcaatt tttgagttat
    10021 ataggaaagg tttaacctgt tcctggctaa aatacatata actggatgat gactaaacca
    10081 aaacacatat gcgttaagta ttgaaacggg cgtatggcac acccgttgtt tttataaata
    10141 tattaaccgt tataaaataa cgtataaaaa gtcaagtgat cacatttcaa atatcaattt
    10201 gatagtatgg gcttggtgat tatttatggg tagcaataaa aggacagtat ttatcatcca
    10261 tagggatagt ctctgtactt ttattcccat tatgctaatg ccttactgaa ttatgaagca
    10321 tttcttaagc atccaacttt agctagatta atggtttatt attttctaca tcttcaatat
    10381 ataaaagcgt attatcaatg gcgtagtaac tgcgtttgtt atgattaaca tcagtaaccc
    10441 aacggaaaac gcccgcgcct gctagtgttg aacagtattc ccgaaatgta gattttccgc
    10501 aaatatgaag caatgcggcc tcttttattt tagcaggatt cttggtcgta ctaactttta
    10561 actggttcct ggttcctctt aataacaaaa ccgtgtcatc gtgagtaata attctgatgt
    10621 tatccgtagc cagataataa atgtaatgtg caatacggtg atgttttaat tctgaataaa
    10681 accaggagaa gttttgctct tttctcactt gctcaaacat cttttgaaaa acaacgacct
    10741 gatccatcag gataataacc tcttgttagt tgtgagactg cgtagtgtgc acgatcggtt
    10801 ttaccacttc aatctggtct gtcctttggc tgtgatatgt acagagtgtg atagagggat
    10861 tacctgaatt ctcccggtga gcattttgca acggaccagc tccggtacaa atgctgttgt
    10921 gggttcagat tataacattc tgtctaaggg gcgggataaa ggtgaaatta gggggcatga
    10981 aagatgactt tataaccttg ctcaccccaa tgttgtaaaa gttcgttttg ccttctcgtt
    11041 ggtgccatgc ctgtccagac aatcaatgtt tgcgtcggga acagttccgg gcgcggcgat
    11101 tcaatgggat cagcaagaac agaaatgtgc catcctgaca atgataaacg ccatgcttcc
    11161 agccacaatc gtgctctctc ttcaacattc cacgccatca gaatggcttc tttaccgggc
    11221 ttacggcgcg tttcgaaaag cgaggttgct gcgtattcaa ttaatgcgcc gtcaaacata
    11281 ctgctcataa tgcgggaggt gttgtgatca agcacgagac gctggcgaac aggaaggtaa
    11341 acatgattaa tcaattgatc tactgggtac tctcgaccca gtgaaataat tctcgcgcgt
    11401 agtttggcag gattagccat acgaagaatt gacatcatct cttcttgcag gcggctccag
    11461 tcatcttccg tatcctggct ggtggtttcc agtaatgctt taactttgcc tacagggacg
    11521 ccattactga tccaacgctt gatctcttcg atgcgttgta tgtcttcttc atcaaagagt
    11581 cggtgtccgc cttcactgcg ctgtggtttt aacaaaccgt agtggcgttg ccaggcccgg
    11641 agagtgacag gattaatccc gcaacgttca gcaacatcac caatgctgta ataagccaca
    11701 attcctcctt gcggtcacaa atctccgtcg ccggtacacg acccaataat actttgtaca
    11761 atatacgcta aaattgtaca aagtataaat aagatttcag ctaaattgga tgaaacatta
    11821 tttttaatgt agattaaatt taaacataac gtattcattt tcacgatgat ttactgaaat
    11881 catgtgaaag aatgtgctga aaattatttt ttctctggcc acgctatgga agggatacca
    11941 ttcaatttag ctttagcaaa cagatctccc tgaaacatct caattcctgc ggattcaagc
    12001 cacatccact cttctggtgt tgccacgccc atagcactga cttgaatttc aagtgatgta
    12061 cagcatttta tgatcgcttg aataattgcc tgccgtggcc cgcttttatg aacattggta
    12121 atcaattcct gactgatttt aattctgtca ggctggaagc gtgacaggag taacaaaccg
    12181 gcaaaacctg cgccaaaatg atcaattgct acactgatac cggcagcctt aagcgattta
    12241 atcgcttctg caaactcatc aaaccgagat ataacttcac tttcagtaaa ttcaacgatg
    12301 atttgttcag gcaccagagc attggccttt atttcatcaa gtaaaaaaga gactgcgtca
    12361 ggttcgttaa ccagggtcat aggtaataga ttgattgaaa tcattttatc accgagctca
    12421 agtgcgtgtg tcatcgcgaa tgcaagcgcc ttacttttga gatccgctgt gtagatttcc
    12481 ccgtctttac gctgcccaac cgctatggct gatgggctat cttcattttt ttgcacaatg
    12541 gcttcaaaag cgattatccg ccgcgataag ggatcgacaa taggatgaaa ggcaaagtgg
    12601 tcgtttatag ttggtgaaag ggtacaggaa tcaagttctt tatcagatcc gtcagcgata
    12661 aaaagccagg agtcttcggc agggatctcg aaataggttg attgttcggt tgcaaggaca
    12721 aaagtacgaa aaaattgtag cgctctgtca tcataagtta gctgaaattt tgatgtgcct
    12781 ttgtcgaata cggcctgtaa aacgtcatct cgctcgtgca ggcgcaaatc aaataattcc
    12841 attcccgctt tgccaaaacg gcgagcaggc gcgtaatcgc acagcagctc aacaatatta
    12901 tagtgccgtg gatcttggca tatagcccga tatatcattt taacctgttc ttccggacct
    12961 tccagaagct ggaaaaaatg agaaccatta aacagtaaga tccctgttac gtcagactgc
    13021 atgttcctgc gatttgctat cgaaaccatt tcttcgattt ttttgacagg ttcgtcgtca
    13081 cgtatatggc tacgataaat aagggtggta agcattaaca atccagggta atgggtgagg
    13141 cgagagtaag acggtaacag acatatcttc ttgtgtcttt cttttaatac caaaacataa
    13201 ccgtttcttt acattgataa aaaatggaaa aagttgaaca cgagttggcg aaaaatcttg
    13261 tatagattgt cagttaaatg atgcaatatg ttttatcata acacattgtt ttatatacat
    13321 tagtactaat tgcaaaaaat taatttatca ttctgtacac atatttcgta caagtttgct
    13381 attgttacct cacttcacat tgattaacat ttttaacaga ggcgtagcat gcatcaaaat
    13441 tcagtgactt cagattctgc gggagcaatc actcgttact ttgcaaaagc taacttgcct
    13501 actcagcagg aaactctcgg ggaaattgtg actgagattt tgaaagatgg acgtaatctg
    13561 agtcgaaagt cgcattgtgc caaacttctt tgccgactgg aacaagcgac aggggaagag
    13621 gaacagaaac attataatgc actaattggg ttgctttttg aataatgcaa cagcctgatt
    13681 atttaacagg ttagctatga agtcgttatg aagacatctg ataatgaacg tataaaatat
    13741 gaaattactg gccaggcggt gctccagata ctgcgcatga agataaattt ttcattgcag
    13801 actcttatta agcaattact cgtaatgaaa tcggctgagg aagatgcttt ccgacgcgat
    13861 ttaattgaca gcataattcg tgattttagc aatagtgatt caggagggcc aaaccgaaga
    13921 acagcgacag ctgacaataa aagtatgttc aatggtaaga aaataaacag aatacattaa
    13981 aatttcataa gtaagatgag aggttaccat gcttgaagat actacaattc ataatgcaat
    14041 aactgataaa gcgttagcaa gttactttcg cagttcgggt aatttgttag aagaagagtc
    14101 agcagtgtta gggcaggctg tcaccaattt aatgctttca ggcgataatg ttaataataa
    14161 aaatattatc ttaagtctga tacactccct ggaaacaaca agtgatattc tcaaagctga
    14221 tgtgattaga aaaacactgg aaatcgtgtt gcgatacaca gctgatgata tgtaacttct
    14281 aattattaag tataagttta tagaaaactc attcatctta tttttgtctg tcgctttaga
    14341 ctatacacag cataatttta ttgggttaat atttctacga ggctgacatg aataattcaa
    14401 tcccagagag atttattttt caatgtgctt tgtttaaaaa tctcgagagg gaggtgttca
    14461 tgacgcatgg gtatgttgat agtcatatta ttgatcaggc tttacgcttg cgtttaaaag
    14521 atgaaaccag tgtgattctt tctgatctct atctgcaaat attgcagtac attgaaatgc
    14581 ataagactac actaacggat atcattatta atgacaggga atccgtgctc tcttagaact
    14641 tgagtaaggt tttagaataa acatcagctg tatcaccatg ctgatgcaaa gtgagcattt
    14701 caggcgttat gctttcttat tatgtccgca atatcaggtg tcaagaatgg agagttctcg
    14761 ctctccattc ttgacgcctg atatcccgcc taacttattt tgtactgcct acattatgct
    14821 caataattaa tgggttagat caagatggag aaaattttat cttcggcgtg gatatttctt
    14881 atgctctcta ccatgcatct ctgtagatgc cagcgcgagc gaatgaatgg cat
//
LOCUS       DAJTNM010000093        14891 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTNM010000093 DAJTNM010000000
VERSION     DAJTNM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.093"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO172_002960"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO172_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5513075.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO172_002961"
     CDS             2219..2767
                     /locus_tag="OO172_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5513076.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO172_002962"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO172_002962"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5513077.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO172_002963"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO172_002963"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5513078.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO172_002964"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO172_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5513079.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO172_002965"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO172_002965"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5513080.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="OO172_002966"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="OO172_002966"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5513081.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12382
                     /gene="pepN"
                     /locus_tag="OO172_002967"
     CDS             9767..12382
                     /gene="pepN"
                     /locus_tag="OO172_002967"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5513082.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12589..13356)
                     /gene="ssuB"
                     /locus_tag="OO172_002968"
     CDS             complement(12589..13356)
                     /gene="ssuB"
                     /locus_tag="OO172_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5513083.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13353..14000)
                     /locus_tag="OO172_002969"
     CDS             complement(13353..14000)
                     /locus_tag="OO172_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5513084.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNI
                     DRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGI
                     GYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(14012..>14891)
                     /locus_tag="OO172_002970"
     CDS             complement(14012..>14891)
                     /locus_tag="OO172_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5513085.1"
                     /translation="RQAAALDRLSNGRALFNLVTGSDPQELAGDGVFLDHSEHYEASA
                     EFTQVWRRLLLGETVNFNGKHIHVRGAKLLFPPIQQPYPPLYFGGSSDVAQELAAEQV
                     DLYLTWGEPPELVKEKIEQVRAKAAAYGRKIRFGIRLHVIVRETNDEAWQAAERLISH
                     LDDETIAKAQAAFARTDSVGQQRMAALHNGKRDNLEISPNLWAGVGLVRGGAGTALVG
                     DGPTVAARINEYAALGIDSFVLSGYPHLEEAYRVGELLFPHLDVAIPEIPQPQPLNPQ
                     GEAVANDFIPRKVAQS"
BASE COUNT         3747 a         3842 c         3780 g         3522 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agtcgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg agaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaattatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgtgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atgggggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ctcttccagc caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccagcgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagaa gagggcgcac tggtcatcag
    10021 taatttgccg gagcgtttta cgcttaagat cattaatgaa ataagcccgg cggcgaatac
    10081 ggcgctggaa gggctttatc agtcaggcga tgcgctttgc acccagtgtg aagccgaagg
    10141 tttccgccat attacgtatt atctcgaccg cccggacgtg ctggcgcgtt ttaccaccaa
    10201 aattattgcc gataaaatca aatatccctt cctgctttcc aatggtaacc gcgttgcgca
    10261 aggcgaactc gaaaacggac gccattgggt acagtggcag gacccgttcc cgaaaccgtg
    10321 ctacctgttt gcgctggtgg caggcgactt tgatgtactg cgcgatacct ttaccacgcg
    10381 ttctggtcgc gaagtagcac tggagctgta cgtcgatcgc ggcaaccttg atcgcgcgcc
    10441 gtgggcgatg acctcgctga aaaactccat gaaatgggat gaagaacgct ttggcctgga
    10501 gtatgacctc gacatctata tgatcgtcgc ggtggatttc ttcaatatgg gcgcaatgga
    10561 gaataagggt ctgaatatct ttaactccaa atatgtgctg gcccgcaccg acaccgccac
    10621 cgacaaagat tacctcgata ttgaacgcgt tatcggccat gaatatttcc ataactggac
    10681 cggtaaccga gtgacctgcc gcgactggtt ccagctcagc ctgaaagaag gtttaaccgt
    10741 cttccgcgat caggaattca gctctgacct tggttcccgc gcagttaacc gcatcaataa
    10801 tgtgcgcacc atgcgcggat tgcagtttgc ggaagacgcc agcccgatgg cgcacccaat
    10861 ccgcccggac atggtcattg agatgaacaa cttctacacc ctgaccgttt acgagaaggg
    10921 cgcggaagtg atccgtatga tccacaccct gctgggcgaa gaaaacttcc agaaagggat
    10981 gcaactctat ttcgagcgtc atgatggtag cgcagcgacc tgtgacgact ttgtgcaggc
    11041 gatggaagat gcgtcgaatg ttgatctctc ccatttccgc cgttggtaca gccagtccgg
    11101 tacgccggtt gtgaccgtca aagacgacta caatccggaa actgagcagt acaccctgac
    11161 catcagccag cgcacgccag ccacgccgga tcaggcagaa aaacagccgc tgcatattcc
    11221 gtttgccatc gaattgtatg acaacgaagg caaagtgatc ccgttgcaga aaggcggtca
    11281 tccggtgaat tccgtgctga acgtcaccca ggcggaacag acctttgttt ttgataatgt
    11341 ctacttccag ccggtgcctg cgctgctgtg cgaattctct gcgccagtga aactggaata
    11401 taagtggagc gatcagcaac tgaccttcct gatgcgtcat gcgcgtaatg atttctcccg
    11461 ttgggatgcg gcgcaaagtt tgctggcaac ctacatcaag ctgaacgtcg cccgtcatca
    11521 gcaagggcag ccgctgtctc tgccggtaca tgtggctgac gctttccgcg cggtgctgct
    11581 cgatgagaag attgatccgg cgctggcggc agaaatcctg acgctgcctt ctgtcaatga
    11641 aatggctgaa ctgttcgata tcatagaccc gattgctatt gccgaagtac gcgaagcact
    11701 cactcgtact ctggcgactg aactggcgga tgagctgctg gctatttaca acgcgaatta
    11761 ccagagcgag taccgtgttg agcatgaaga tattgcaaaa cgcactctgc gtaatgcctg
    11821 cctgcgcttc cttgcttttg gtgaaacgca tctggctgac gtgctggtga gcaagcaata
    11881 ccatgaagca aacaatatga ccgatgcgct ggcagcgctt tctgcggcgg ttgctgcaca
    11941 gctgccttgc cgtgacgcgc tgatgcagga gtacgacgat aagtggcatc aggacggtct
    12001 ggtgatggat aaatggttta tcctgcaagc caccagcccg gcggcgaatg tgctggagac
    12061 ggtgcgtggt ctgttgcagc atcgctcatt taccatgagc aacccgaacc gtattcgttc
    12121 gttgattggt gcgtttgcgg gcagcaaccc ggcagcgttc catgccgaag atggcagcgg
    12181 ttaccagttc ctggtggaaa tgcttaccga cctcaacagc cgtaacccgc aggtagcttc
    12241 acgtctgatt gaaccgctga ttcgcctgaa acgttatgat gccaaacgtc aggagaaaat
    12301 gcgcgcggcg ctggaacagt tgaaagggct ggaaaatctc tctggcgatc tttacgagaa
    12361 gataactaaa gcactggctt gatacttttg agccaaaatg cctgatgcga cgcttgacgc
    12421 gtcttatcag gcctacaact ccgttgaaat tgctggtttt gtaggctgga taaggcgttc
    12481 acgccgcatc cggcagccgt gcccaactgc ctgatgcgat gcttgacgtg tcttatcagg
    12541 cctacaagtc cgttgaaaat gctggttttg tgcgccacat ccggcatatt aaccatgacg
    12601 ccgaatcagt tgttcagaat gcccacgccg cattacccgt tgcaatactt ccgcttccag
    12661 ctccgctaat cgcaccgacc ctaatcgacg cggacggggg atatccactg tcaaatccag
    12721 actaattttt ccctcttcaa ttaacaacac ccggtcagcc attgctaccg cttcgctcac
    12781 atcatgcgtc accaacagta cggtaaagcc gtgctgctgc caaagtgaca caatcaaatc
    12841 ctgcatctcg agtcgcgtta aggcgtccag tgccccgagc ggttcatcaa gcaacaataa
    12901 acccggtcga tgaatcaacg ctcttgccag cgccacacgc tgtttctgcc cgccagaaag
    12961 tgcggcaggc cattccccgg cgcgattctc cagtcctacc gcagccaacg cccgacgcgc
    13021 ggcatcgcgc cactgacctt taaggcctaa cccaacgtta tcaatcaccg atttccacgg
    13081 cagcagacgc gcatcctgaa acatcattcg cgtatcatcc tgaatttcag ccagcggtgt
    13141 ggtgcccgct aacacatcgc ctgcggttgg cgtttccaga ccggccagca ggcgcagcag
    13201 ggtactttta ccaccaccgc tgcggcccac caccgccaca aactgacctg ccggaatatg
    13261 taaatccagt tggttcagga cgatattttc cgcgtaatgt ttgcttactg cattgagcaa
    13321 caatggtgtg ccctggttca gacgagcagt attcatatcg tggcctcctt caaatgataa
    13381 gccgggttcc agctcaacca gaggcgctct aacagctgcg cgctgacgtc agcaagtttg
    13441 ccgagcaggg cgtaaagaat aatagcgact accaccacgt ctgtttgcaa aaactctcgc
    13501 gcattcatcg ccagataacc aatgccggaa ttggcagaaa tggtttcggc aacaatcagc
    13561 gtcagccaca tcaggcccaa cgcaaaacgc acgccgacca taattgaggg cagggcatcg
    13621 ggcaggatca catggataaa cagcggtatg ccggataatc catagctacg cgccatctcc
    13681 accagcccgc gatcgatatt acggatgcca tgccaggtgt tgatataaat ggggaacagc
    13741 gtaccgagcg ccaccagaaa gattttcgcg gattcatcaa tgccaaacca caaaatcacc
    13801 agcgggatca gcgccagatg ctgccacagt tcgccgctgg cggagagcgt ccagaacgcc
    13861 gtcaccactc cttccggtga aggcaaaata cgcgtcgaca gccagccaac cgaggaggcc
    13921 agttgccaca ccgccacgat gcccaccggt aaaaaccagg gggcaacgcg caataaccac
    13981 ttcttcactg gcgttgccat catcgtgttc cttagctttg cgcgacttta cgggggataa
    14041 aatcattcgc caccgcttcg ccttgcggat tcagcggctg cggctgggga atttccggaa
    14101 tggcgacatc cagatgtggg aacagcaact cgccaacccg atacgcttct tccagatgcg
    14161 ggtagcccga aagcacaaag ctgtcgatgc caagcgcggc gtattcgttg attcgcgcgg
    14221 cgaccgtagg accatcgccc accagcgccg ttccggcacc gccgcgcact aagccaacgc
    14281 ccgcccataa attggggctg atctccagat tgtcgcgctt gccgttatgt agcgccgcca
    14341 ttcgctgttg tcctacggaa tccgtccggg cgaatgcggc ttgtgctttg gcgatagtct
    14401 catcatcaag atgcgagatt aaccgctcgg cggcctgcca cgcttcgtcg ttagtttcac
    14461 gaacaatcac atgcagacga ataccgaaac gaattttgcg cccatacgcg gcagctttcg
    14521 cccgcacttg ttcgattttc tctttaacaa gttccggcgg ttcgccccag gtgaggtaga
    14581 gatcaacttg ttctgccgcc agctcctggg cgacatctga cgatccgcca aagtaaagcg
    14641 gaggataagg ctgttgaatc ggcgggaaga gcagttttgc tccgcgcaca tgaatatgtt
    14701 taccgttgaa attgacggtt tcccccagca ataagcgccg ccagacctgg gtaaattccg
    14761 ccgaggcttc gtagtgctcg ctatgatcaa ggaacacgcc gtcgcctgcc agctcttgtg
    14821 gatcgctgcc tgtgaccaga ttaaacaacg cacgaccatt tgagagacgg tcaagcgcgg
    14881 cggcctggcg a
//
LOCUS       DAJTNM010000094        14864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTNM010000094 DAJTNM010000000
VERSION     DAJTNM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.094"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..266
                     /locus_tag="OO172_002971"
     CDS             <1..266
                     /locus_tag="OO172_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCR5513086.1"
                     /translation="DHLRQTALRNAHTPASLLTTLTEPQDRSLAINNPQLAADVKTAW
                     LKEDPSLLLFVEQPDLSQLRDLVKTGATRKIRSEARHRLEEKQ"
     gene            263..718
                     /locus_tag="OO172_002972"
     CDS             263..718
                     /locus_tag="OO172_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513087.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(796..1707)
                     /gene="rdgC"
                     /locus_tag="OO172_002973"
     CDS             complement(796..1707)
                     /gene="rdgC"
                     /locus_tag="OO172_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5513088.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1832..2740
                     /gene="mak"
                     /locus_tag="OO172_002974"
     CDS             1832..2740
                     /gene="mak"
                     /locus_tag="OO172_002974"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5513089.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2883..4064)
                     /gene="araJ"
                     /locus_tag="OO172_002975"
     CDS             complement(2883..4064)
                     /gene="araJ"
                     /locus_tag="OO172_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5513090.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(4190..7333)
                     /gene="sbcC"
                     /locus_tag="OO172_002976"
     CDS             complement(4190..7333)
                     /gene="sbcC"
                     /locus_tag="OO172_002976"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5513091.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7330..8532)
                     /gene="sbcD"
                     /locus_tag="OO172_002977"
     CDS             complement(7330..8532)
                     /gene="sbcD"
                     /locus_tag="OO172_002977"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5513092.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8722..9411
                     /gene="phoB"
                     /locus_tag="OO172_002978"
     CDS             8722..9411
                     /gene="phoB"
                     /locus_tag="OO172_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5513093.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9469..10764
                     /gene="phoR"
                     /locus_tag="OO172_002979"
     CDS             9469..10764
                     /gene="phoR"
                     /locus_tag="OO172_002979"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5513094.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            11171..12490
                     /gene="brnQ"
                     /locus_tag="OO172_002980"
     CDS             11171..12490
                     /gene="brnQ"
                     /locus_tag="OO172_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5513095.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12566..13939
                     /gene="proY"
                     /locus_tag="OO172_002981"
     CDS             12566..13939
                     /gene="proY"
                     /locus_tag="OO172_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5513096.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            14095..>14864
                     /locus_tag="OO172_002982"
     CDS             14095..>14864
                     /locus_tag="OO172_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HCR5513097.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDS"
BASE COUNT         3292 a         3787 c         4000 g         3785 t
ORIGIN      
        1 atgatcattt gcgccaaacg gcattacgca acgcacatac cccggcgtcg ttgttaacga
       61 cattgactga gcctcaggat cggtcgctgg ctattaataa tccacagctg gctgccgatg
      121 tgaaaacggc gtggttaaaa gaggatccat cattactctt atttgtcgaa caacccgatc
      181 tttcgcagtt acgtgattta gtgaaaaccg gggcaacgcg gaaaattcgc agtgaagcgc
      241 gtcaccggct tgaggaaaaa caatgacaca acgtccatgg agcaagttac agcgggagat
      301 ctatgactta ctgacgccaa cgatcaacct gcaaattcac tgtacccgtt atcccatgcg
      361 cagccaaaat ggtggaagta ccgatttacc gcgttattgg atcacgttgg ataaaaacgt
      421 gatctgggat tacccgaaag actttattgc aggtaacgct ggagtgcgta attttcacgg
      481 tgaaacttgc tggtatcctt acctgacgga tatttgttca atttctgatt tattgcgtga
      541 atatattgat accccaaaag cggagctgtt aacaaaacaa tttaccagcg ataaatgggg
      601 gttagtgaat attctgcgtg ctgctgacag gcgaataggg atgaggcgat tagatcagtt
      661 acggcgtaaa acgcataata ttgctgcgct aaaaattatt gctcgccgta gcgaataatt
      721 atatgcctgg tgtggcttcg tacgccggat aagacgcggc aggcgtcgca tccggcatta
      781 aaggaaaatc agcaattaac gttgtgcttc gccacctaat ccttcaatca ggttttgaat
      841 taacgctgcc agttcaccag tcatcaggat gaaatcggca tcaaaacgct gggcgaaatc
      901 ttcacggtcg atatcttcgt tttgatcgtg cagctcgtcg cagaacttaa gacgcttgag
      961 cgaaccatcg tcgcacatca caaactgaat gcgctgctgc cagtcgagcg ccagtttagt
     1021 caccactttt ccggcttcaa tgtgattggt gatctcttcg ctggtcagat cttgtttctt
     1081 cgcgcggatc acgccgccat cttccagcaa cgatttcagc tcggcttcat caagcagctg
     1141 gaagccctgt gccgcactac cggagcgaac ccattcggtc agcgtcagtt caatcgggtt
     1201 ttccatgctc aatggtacaa ccggtaacga ccccaggctt ttacgcagta atgccagcgt
     1261 atcttccgct tttttggcac tggcgcagtc caccataatc aaaccgttaa ccgtgtcgag
     1321 ccacatcatt gtctggctaa aacggctgaa agcacgcggc agcagagagt gcagcacttc
     1381 gtctttcagc gaatcttttt cggttttctt cagcttacgc gcctgttccg cttccagttt
     1441 ggcgattttc gcttccagcg cctgtttaat caccggagac gggaggattt tttcttcttt
     1501 gcgcgcgcag ataacaattt gaccattggc aacgtgcgtt aacgcatcgc tgtgcgatcc
     1561 catcggagga acccagccca tcttcgccat gtcctggctg ccgcatgggg taaatgccat
     1621 cgaggctagc tgtttttcca tctcttctgc acgcagcgaa atctcgcggc taagacggta
     1681 aaccattaaa tttttgaacc acagcatgat aatttccacg gccttgtcgt taaatttagc
     1741 gggcatgata acgaattgtc ggcggccttg cattgccaat ccggttgtcc gtctctacgc
     1801 tattgatatt gaaaaaaata aggagagtac agtgcgtata ggtatcgatt taggcggcac
     1861 caaaactgaa gtgattgcac tgggcgatgc aggggagcag ttgtaccgcc atcgtctgcc
     1921 cacgccgcgt gatgattacc ggcagactat tgaaacgatc gccacgttgg ttgatatggc
     1981 ggagcaggcg acagggcagc gcggaacggt aggtatgggc attccaggtt cgatttcgcc
     2041 ctacaccggt gtggtgaaga atgccaattc aacctggctc aacggtcagc cattcgataa
     2101 agacttaagc gcgaggttgc agcgggaagt gcggctggca aatgatgcca actgtctggc
     2161 ggtttcagaa gcagtagatg gcgcggcagc gggagcgcag acggtatttg ccgtgattat
     2221 cggcacggga tgcggcgcgg gcgtggcatt caatgggcgg acgcatatcg gcggcaatgg
     2281 cacggcaggt gagtggggac acaatccgct accgtggatg gacgaagacg aactgcgtta
     2341 tcgcgaggaa gtcccttgtt attgcggtaa acaaggttgt attgaaacct ttatttcggg
     2401 cacgggattc gcgacggatt atcgtcgttt gagcggacat gcgctgaaag gcagtgaaat
     2461 tatccgcctg gttgaagaaa gcgatccggt agcggaactg gcattgcgtc gctacgagct
     2521 gcggctggca aaatcgctgg cacatgtcgt gaatattctc gatccggatg tgattgtcct
     2581 ggggggcggg atgagcaatg tagaccgttt atatcaaacg gttgggcagt tgattaaaca
     2641 atttgtcttc ggcggcgaat gtgaaacgcc ggtgagtaag gcgaagcacg gtgattccag
     2701 cggcgtacgc ggcgctgcgt ggttatggcc acaagagtaa aaaacgtagg caattggcgc
     2761 atcatgcctg atgcgacgct tgccgcgtct tatcaggcct acaaaatgtg ccagaaccgc
     2821 gtagggcgga taaggcgttc acgccgcatc cggcaataag caacgcgcac cgtgacagta
     2881 atctacccca gtggtttcgc cagcaccgga ctatccgccg cttgctggcg cttatagcga
     2941 ccatacagca gcaacgatga catcgcagca aacgaaaaca gggcggcagg cagtgccacg
     3001 taattatatg ccagccccag cgtcagcatc atacctccgc aatatgcgcc gacggcgcta
     3061 ccgaggttaa acgctatttg cccacctgcg gcacctaata actctccgcc tttggcattt
     3121 tgcagtaaca atatttgcag aggtgctgaa agggcaaata atcccgcgca acaaataaaa
     3181 gcaaaaataa gcgacgttgt tttcatgccg ccgcagaaaa agagcatcag cagtgccagt
     3241 acaatgataa agtcagtcac tgctgcaatg cgcagtggtg aataacgtcc tgaaatcctg
     3301 ccacttagca tatttcccag caccatccct agcccaacta acatcataat aaaggtcatc
     3361 gccgtttccg aaaaaccgga aataaacatc atgtatggct ttacgtagct gaaccaggca
     3421 aacacacctg cgttgccaaa catcgtggcg gcgaaaatta accacggggc cgggctgcgc
     3481 aaaaagtgaa attgttcgcg cagatttcct ttcgcctcgt cgcgaatatc tggcacccaa
     3541 aaatagaccg atgccatcac cgcaatatta aaaacagcga tcaataaaaa ggtgtaacgc
     3601 cagcattcct gacttaaata cgttcccagc ggaatgccca gcaaattggc gactgtcatc
     3661 ccggaaacca tccccgccac ggcggcggtg acttttccgg gtttgataat ttttgataac
     3721 acgatcgctc cgacgccaaa aaatgcgcca tgcggaaagc cggataccag ccgaccaatg
     3781 gcgagcatca ggtaagacga agagagcgtg aacatggcgt tgccaatgac gcacaacgcc
     3841 accagaaaca acaagatatg tttgagtgag tagcggctgg aaaagagtgc gatgattggc
     3901 gcaccgacca ccaccaccag tgcataatac gagatcatat gcccggcagc aggaatcgaa
     3961 attcctacgt tatgcgccag ctccgtgagc acgctcataa tgccaaattc ggctatcccc
     4021 aaaccaaacg tgcccagagc caaagataaa atgacttttt tcataccacc atcacaacca
     4081 ccagaacggc taccaatcac cgtagccaat aaagtgatag cttgcaggat aacgaccagc
     4141 aaggccaaga gataattcgc ccctctgtat tcattatcct gctgaatagt tatttcactg
     4201 caaacgtact ttccagtttg ctgtagccca ggccgttgat ctttttcact ttgatctgca
     4261 ccggaatacg ctctttcatc gcttctacgt ggctaatcac accgatggtt ttgccactgg
     4321 cgttcagggc atccagcgca tcaagggcgg tatccagcgt ttcgctatcc agcgtgccaa
     4381 aaccttcatc aaggaacagc gagtcaatac gtgttttatg gctgaccaga tccgaaagcg
     4441 ccagcgccag cgccagacta acgaggaaac tttcgccgcc ggaaagggta cgggtatcgc
     4501 gtaccgcatc tgcctgccag gtatcaacaa cctcgacttc cagcgcctcg ctggctttgc
     4561 gctgtaacag atagcgcccg tgcagccggg taagttgctg attagcgaga tggactaaat
     4621 tatccagcgt cagcccctgg gcaaacttgc ggaatttatc gccctctttg gaacctatta
     4681 gcgaattcag atatccccag tcctcaacct gctgcgtcat ttgagcaatt tgctgcatta
     4741 aggtttgttg ttgctgacgg ttatctgcat cctgcttcag ctgctggcga atctcgcctt
     4801 gactcgtggt gttttcacgc aacttttggt gagtttgcgc taactcttgc tgaatctgct
     4861 ccaccgtcac agtgagagcc aacccgtcag gtcggtgttg ttgatgctgt gccagcgttt
     4921 ctgctgtctg agtgaccagc gtttgcgcct gacggcgctg gttttccaga ttctgcttga
     4981 gctgttccag ctgcgttagt gtttgttcat ccattagcgc cgcaaggaac gcctgctgat
     5041 cgtcaaagac gctggcctgt agcgtggtgt caaactgcgc ttgggctttt tgcagacttt
     5101 gcgccgccag aacatcctgt tgctgtaacg tctgctgctg gctgtgtaat gcgagacatt
     5161 gttcatgaac ctgccgccag ttatccagcg ccacagtttc ttcgctgtgc gggagatcat
     5221 cactttgcgg caacgtttcc agaatcggcg tcagctgctg aatacggttt tgcagcgcgg
     5281 ttaattcgtt ctggcgttgc tgccagctct gcgcttcttg ctgacgtgtc gccaaccagc
     5341 tctcttcttc atcttcctgt ggcaatgtca gtgcataacc cgccaatgcc gttaagagct
     5401 gctgctgacg ttgttcaatt tgctgttgat actggataat ttgctgatta tgcgcggcaa
     5461 tctgcccttg taattcatgt cgttggctga gtaaccgcag ctggcgttcg tgctcatctt
     5521 gtgcatccag ccacggttga atatcgtcct gtggctgcaa ggtgatattg aggctggccg
     5581 tgacggcttg ccattgttga gtaagtgctt gctcatcttg tcggaggctt tgcgcttcgt
     5641 tttcatcgcg ctgaagctgc ttcgttaatg catccagttg cccgcgcagc gccgcacctt
     5701 cttcgccgag ctttttaact tcgttttcca gcgccagtaa tcgagactga ttaacaccag
     5761 gctccagcgc ctgatacgcc tcgaccgccg ggtggctggt ggaaccacaa agtggacaag
     5821 gctgacccgc ctgtaactgt gcacgctggg cttccagcgt tttgatgcgc gcttcctgct
     5881 cgcaaatggt tttcacatcg gcaagttgct gcgtcttttc tttataacgc tggcgcattt
     5941 cgttaagtgc gacattacgt tgcgtctgtt ccagagtgac attctggata gtgaccatta
     6001 actgcgccag acgtttttgt tggggaacaa tctgcccatg cagcgcgacc aggcgctgac
     6061 gcagtgggcg ttgctcggca tgttgcgcca gggcggtagc aacttcatcg gcggttaacg
     6121 tcaacgtgat cgccgcaagc gcattaagtt tttgctcagc atgggttaac tgttgctgcc
     6181 attgccgcag atgctcgcga tcgctggttt gttgggagaa ctgcgcacgc caacccgcca
     6241 gttcgttgtt ccactgacgg aagcggtcgt gttcctgtaa ccaggtattc aggctttgtt
     6301 gctgctgctg taattctgct gactgcttcg ccgcgtggtg gcgaatgctc gcgcgaagcg
     6361 ccattgtgct ctgtaagcga gtatttactt cttcaatctg ctggcgaata tgcgccagcg
     6421 ccgcgctgtg ttctgcgatg cgttcccagt gtggacgaag atttcgtgcc ggttgtgcca
     6481 gactaagcgc cgccagttga ggttgcgctt tttcttcttc ggctaacgcc tgttgcaagg
     6541 cctgctgacg gcggctggct tcttgctgca attcgtcctg acgcgttaac cagtttagcg
     6601 attgttgttc ttgctgctgc gcggtaatta actgtttttc ttcgtcagta agtacctgca
     6661 aactcgctgt cagcgattgc acttgttccg gcgtgagcaa cgtgacgccg ctggcctgcg
     6721 cttgcagctt ctccagctca gtacgggccg atttgtgctg ctcaaaaacc atcgccgaga
     6781 tttgcccgta gatttcagtg ccggttaact cctcgagcaa ttccgcgcgt tctttgggtt
     6841 tggcattcag gaaggcagca aattgcccct gcgaaagcag catcgaacgg gtgaagcgcc
     6901 cgtaatccag cccggttaac gtcgctgtca gttccagctt atctttcact ttgtcggcga
     6961 gaattttgcc gtcggcgcag cgcgccagct ctacgcgagg cacctgcaaa ttaccgtccg
     7021 gctggttacg cgcccgattc tggctccaga acgcgcgata cgcttcacct ttcacttcaa
     7081 actccacctc cgccagacat tcagcggtat cgcgggtcat gagatcattt tgcgattgtg
     7141 aaacgttaga gagacgcgga gtttcgtgat acagcgccag acaaatggcg tccagcaggg
     7201 tggttttccc cgcaccggtt gggccggtaa tggcaaacag cccgttgctg gcgaacggct
     7261 cgcgggtgaa atcaatcttc cattcgcctt ttaatgagtt caggtttttc aggcgcaggc
     7321 tgagaatttt catgcttcgt gttctccggc gagggtatgc aacgtcgtgg tgaaaagatg
     7381 ctgcagacgt tgctgctgcg attcatccag ttcttccagt gccagacggc gattgaacac
     7441 ctcttcgacg ctgagttcgc tgagggtttc acgctgttgg ctggctaaca cacgctcgcg
     7501 ctgttcacga ctccgacgta ccagcaatac ttcgacaggc aatgattcgg ttaatgcctg
     7561 gattttgcgc tgaatatcat gcagatactc atcagtagtg atttcgatat ccagccagac
     7621 aggtggctcc tgcgatacat cgcgccactg ttccagctgt gcggtaatcg acgccagatc
     7681 gcctttcagc accgccatgg gttgagttac cggtacgttc aggttttcca cgctctctaa
     7741 tttgccgttt gaaaatgtca ccagatggac atatttactc ttaccgcatt catcaaaact
     7801 cagtggaatg ggggaaccgc aatagcgaac atgttccatg ccgccaataa tctgtgcgcg
     7861 gtgaatatgc ccgagcgcga tgtagtcggc tggtggaaag ttttgtgccg gaaacgcgtc
     7921 cagcgtgcca atataaatgt cacgcacggc gtcactttta ctggcaccca cggtcgttaa
     7981 atgtcccgtg gcgatgatgg gcagaggctg atcgccgcgc agtttgcagg catcggcata
     8041 gtgttgttgg taataatcgg taatcgctgc cagtaaatgc tgctgttttt caataccgtt
     8101 aagccccgcc tggctggaaa taatgtcacg cgggcgtaaa aacggaatgg ggcacagcac
     8161 tgcgcctggc gtcccgtcgc gacgaggcaa gatttgcggc gcatgtccgg cgctggcgac
     8221 cacggttgta ttgaggaacg ccatgatatc gcgcgattca ttcagcgtgg cgaccgagtc
     8281 atggtttcct gccagtacca ccagatgaca gccagtttgc tgtaaattga caacaaaacg
     8341 gttgtataac gtgcgggcgt aactgggcgg cgagccggta tcgaaaacat caccagcaac
     8401 aataatcgca tccacctgat gggtttgtgc tgtctccagc agccagtcaa gaaaagcctg
     8461 atgttcagct tcgcggcttt tactgtagaa gttctggccg agatgccagt ctgaggtgtg
     8521 aaggatgcgc ataacggttc cctggcgaaa aagcatggtc gcgattatac ccaaacagat
     8581 gtgccatttg cttttttctg cgccacggaa atcaataacc tgaagatatg tgcgacgagc
     8641 ttttcataaa tctgtcataa atctgacgca taatgacgtc gcattaatga tcgcaaccta
     8701 tttattacaa cagggcaaat catggcgaga cgtattctgg tcgtagaaga tgaagctcca
     8761 attcgcgaaa tggtctgctt cgtgctcgaa caaaatggct ttcagccggt cgaagcggaa
     8821 gattatgaca gtgctgtgaa tcaactgaat gaaccctggc cggatttaat tctccttgac
     8881 tggatgttac ttggcggctc cggtatccag ttcatcaaac acctcaagcg cgagtcgatg
     8941 acccgggata ttccagtggt gatgttgacc gccagagggg aagaagaaga tcgcgtgcgc
     9001 ggccttgaaa ccggcgcgga tgactatatc accaagccat tttcgccgaa ggagctggtg
     9061 gcgcgaatca aagcggtaat gcgccgtatt tcgccaatgg cggtggaaga ggtgattgag
     9121 atgcagggat taagtctcga cccgacatct caccgagtga tggcgggcga agagccgctg
     9181 gagatggggc cgacagaatt taaactgctg cactttttta tgacgcatcc tgagcgcgtg
     9241 tacagccgcg agcagctgtt aaaccacgtc tggggaacta acgtgtatgt ggaagaccgc
     9301 acggtcgatg tccacattcg tcgcctgcgt aaagcactgg agcccggcgg gcatgaccgc
     9361 atggtgcaga ccgtgcgcgg tacaggatat cgtttttcaa cccgctttta acgccttgct
     9421 catcggacgc agagcagggc ttatgatttc ttaactggag tatcttacgt gctggaacgg
     9481 ctgtcgtgga aaaggctggt gctggagctg ctactttgct gcctcccggc tttcatcctg
     9541 ggtgcatttt ttggttacct gccctggttt ttgctggcat cggtaacagg actgcttatc
     9601 tggcatttct ggaatttatt gcgcctttca tggtggctgt gggtggatcg cagtatgacc
     9661 ccgccaccgg ggcgtggtag ctgggaaccg ctactatacg gcttacacca gatgcagctg
     9721 cgaaataaaa aacgccgccg tgaactgggc aatctgatta aacgctttcg tagcggcgcg
     9781 gagtcgctgc ccgacgcggt ggtgctgacc acggaagagg gcggtatttt ctggtgtaac
     9841 ggtctggcgc aacaaattct tggtttgcgc tggccggaag ataacgggca gaacatcctt
     9901 aacctactgc gttacccgga gtttacgcaa tatctgaaaa cgcgtgattt ttctcgcccg
     9961 ctcaatctgg tgctcaacac cgggcggcat ctggaaattc gcgtcatgcc ttatacccac
    10021 aaacagttgt tgatggtggc gcgtgatgtc acgcaaatgc atcaactgga aggggcgcgg
    10081 cgtaactttt ttgccaacgt gagccatgag ttacgtacgc cattgaccgt gttacagggt
    10141 tacctggaga tgatggatga gcagccgctg gaaggcgcgg tacgcgaaaa agcgttgcac
    10201 accatgcgcg agcagaccca gcggatggaa gggctggtga agcagttgct aacgctgtcg
    10261 aaaattgaag cctcgccgac gcaattgctc aatgaaaagg ttgatgtgcc gatgatgctg
    10321 cgcgttgttg agcgcgaggc tcagactctg agtcagaaaa aacagacatt tacctttgag
    10381 atagataacg gcctcaaggt gtctggcaac gaagatcagc tacgcagtgc gatttcgaac
    10441 ctggtctata acgccgtgaa tcatacgccg gaaggcacgc atatcaccgt acgctggcag
    10501 cgagtgccgc acggtgccga atttagcgtt gaagataacg gaccgggcat tgcaccggag
    10561 catattccgc gcctgaccga gcgtttttat cgcgttgata aagcgcgttc ccggcaaacc
    10621 ggcggtagcg gattagggtt agcgatcgtg aaacatgcgg tgaatcatca cgaaagtcgc
    10681 ctgaatattg agagtacagt aggaaaagga acacgtttca gttttgttat cccggaacgt
    10741 ttaattgcca aaaacagcga ttaatccgcc tttgtcatct tttattgcca taagccagcc
    10801 gatgctggct tattttcttt gcagtcaaaa tacgggcgtt agattttaca acggttggtg
    10861 attttttgtt cgcatgatta gccatgtctt tttcacggaa atagtgtttt atactggttg
    10921 gtgatttctt atcgctatat acctctggtt tttagatccc tccttgcttt aaaacgttat
    10981 aagcgtttaa attgcgcttc aggtgctgtc atactgactg cattaacgcg gtaaatcgaa
    11041 aaactattct tcgccgcgcc tggttgggag tattttccgc taaaattgtt taaatatacc
    11101 gctgtatcat ccccagggat tggcacaaaa atttaacgtt acaacaccac atccacaggc
    11161 agtatgattt atgacccatc aattaagatc gcgcgatatc atcgctctgg gctttatgac
    11221 atttgcgttg ttcgtcggcg caggtaacat tattttccct ccaatggtcg gcttacaggc
    11281 aggcgaacac gtctggactg cggcattcgg cttcctcatt actgccgttg gcctgccggt
    11341 attaacggta gtggcgctgg caaaagttgg cggcggtgtt gacagcctca gcacgccaat
    11401 cggtaaagtc gctggcgtac tgctggcaac ggtttgttac ctggcggtgg ggccgctttt
    11461 cgctacgccg cgtacagcta ccgtttcctt tgaagtgggg attgcgccgc tgacgggtga
    11521 ttccgcgctg ccgctgttta tctacagcct ggtctatttc gctatcgtta ttctggtttc
    11581 gctctatccg ggcaagctgc tggataccgt gggcaacttc cttgcgccgc tgaaaattat
    11641 cgcgctggtc atcctgtctg ttgtcgcgat tgtctggccg gcgggttcta tcagcacggc
    11701 gactgaggct tatcaaaacg ctgcattttc taacggcttc gttaacggct atctgaccat
    11761 ggatacgctg ggcgcaatgg tgtttggtat cgttattgtt aacgcggcgc gttctcgtgg
    11821 cgttaccgaa gcgcgtctgc tgacccgtta taccgtctgg gctggcctga tggcgggtgt
    11881 tggtctgact ttgctgtacc tggcgctgtt ctgtctgggt tcagacagcg cgtcgctggt
    11941 cgatcagtct gcaaacggcg ctgctattct gcatgcttac gttcagcaca cctttggcgg
    12001 cggcggtagc ttcctgctgg cggcgttaat cttcatcgcc tgtctggtca cggcggttgg
    12061 cctgacctgt gcttgtgcag aattctttgc ccagtacgta ccgctctctt atcgtacgct
    12121 ggtgtttatc ctcggcggct tctcgatggt ggtgtctaac ctcggcttaa gccagctgat
    12181 ccagatctcc gtaccggtgc tgaccgctat ttatccgccg tgtatcgcac tggttgtatt
    12241 aagttttaca cgctcatggt ggcataattc gtcccgcgtg attgctccgc cgatgtttat
    12301 cagcctgctt tttggtattc tcgacgggat caaagcatct gcattcagcg atatcttacc
    12361 gtcctgggcg cagcgtttac cgctggccga acaaggtctg gcgtggttaa tgccaacagt
    12421 ggtgatggtg gttctggcca ttatctggga tcgcgcggca ggtcgtcagg tgacctccag
    12481 cgctcactaa atcactgaac atttgtttta accacggggc tgcgatgccc cgtggttttt
    12541 tattgtgttg atgggttagg aattgatgga aagtaagaat aagctaaagc gtgggctaag
    12601 tacccgccac atacgcttta tggcactggg ttcagcaatt ggcaccgggc tgttttacgg
    12661 ttcggcagac gccatcaaaa tggccggtcc gagcgtgttg ttggcctata ttatcggtgg
    12721 tatcgcggcg tatatcatta tgcgtgcgct gggggaaatg tcggtacata acccggccgc
    12781 cagctctttc tcgcgttatg cgcaggaaaa ccttggcccg ctggcaggtt acattaccgg
    12841 atggacctac tgctttgaaa tccttattgt cgccatcgcc gatgtgaccg cttttggtat
    12901 ctatatgggg gtcttgttcc cgacggtgcc gcactggatt tgggtactga gcgtggtgct
    12961 gatcatttgc gccgtaaacc tgatgagcgt gaaggtcttc ggtgagctgg aattctggtt
    13021 ctcgttcttt aaagtcgcca ccatcatcat catgattgtc gccggtttcg gcatcatcat
    13081 ctgggggatt ggcaacggcg ggcaaccgac cggtattcat aacctgtgga gcaacggcgg
    13141 cttcttcagt aacggctggc ttggcatggt aatgtcgttg caaatggtga tgtttgctta
    13201 cggtgggatc gaaattatcg ggattaccgc cggtgaagcg aaagatcctg agaaatcgat
    13261 tccgcgtgcg attaactccg tgccgatgcg tattctggtg ttctacgtcg gtacgctgtt
    13321 cgtcattatg tctatctacc cgtggaatca ggttggcact gccggtagcc cgttcgtgct
    13381 gacgttccag catatgggca ttacctttgc cgccagcatt cttaactttg ttgtgctgac
    13441 cgcttcgctg tcggcaatta acagtgacgt atttggcgta ggccgtatgc tccacggtat
    13501 ggcagagcag ggcagcgcac cgaaaatttt cagcaaaacg tcgcgtcgcg gtattccgtg
    13561 ggttacggtg ctggtgatga ctaccgcgct gctgtttgcg gtgtatctga actacatcat
    13621 gccggaaaac gtcttcctgg tgatcgcttc gctggcaacc ttcgccacgg tatgggtgtg
    13681 gattatgatc ctgctgtcgc aaatcgcctt ccgtcgccgt ttgccgccag aagaagttaa
    13741 ggcgctgaaa tttaaagtgc cgggtggggt agcaacgacc atcggcgggc tgattttcct
    13801 gctctttatt atcgggttga ttggttatca cccggatacg cgtatctcgc tgtatgtcgg
    13861 tttcgcgtgg attgttgtgc tgttgattgg ctggatgttt aaacgccgcc acgatcgtca
    13921 gctggctgaa aaccaataat acttgcgttc tgtaagccgg ttcaggagga aatcctgatc
    13981 cggctattcc gaaagttatc cgcccccgtc ctcctccccc aaatatcctt cagatgatga
    14041 gtgatcctgc attaggctat ggcaaggtga tcagattttc atcacagggg aattatgatg
    14101 ttaaatgcat ggcacctgcc ggtgccccca tttgttaaac aaagcaaaga tcaactgctc
    14161 attacactgt ggctgacggg cgaagaccca ccgcagcgca ttatgctgcg tacagaacac
    14221 gataacgaag aaatgtcagt accaatgcat aagcagcgca gtcagccgca gccaggcgtc
    14281 accgcatggc gtgcggcgat tgatctctcc agcggacaac cccggcggcg ttacagtttc
    14341 aaactgctgt ggcacgatcg ccagcgttgg tttacaccgc agggcttcag ccgaatgccg
    14401 ccggcacgac tggagcagtt tgccgtcgat gtaccggata tcggcccaca atgggctgcg
    14461 gatcagattt tttatcagat cttccctgat cgttttgcgc gtagtcttcc tcgtgaagct
    14521 gaacaggatc atgtctatta ccatcatgca gccggacaag agatcatctt gcgtgactgg
    14581 gatgaaccgg tcacggcgca ggcgggcgga tcaacgttct atggcggcga tctggacggg
    14641 ataagcgaaa aactgccgta tctgaaaaag cttggcgtga cagcgctgta tctcaatccg
    14701 gtgtttaaag ctcccagcgt acataaatac gataccgagg attatcgcca tgtcgatccg
    14761 cagtttggcg gtgatggggc gttgctgcgt ttgcgacaca atacgcagca gctgggaatg
    14821 cggctggtgc tggacggcgt gtttaaccac agtggcgatt ccca
//
LOCUS       DAJTNM010000095        14864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTNM010000095 DAJTNM010000000
VERSION     DAJTNM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.095"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            597..794
                     /locus_tag="OO172_002983"
     CDS             597..794
                     /locus_tag="OO172_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513098.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO172_002984"
     CDS             complement(744..1937)
                     /locus_tag="OO172_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5513099.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO172_002985"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO172_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5513100.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO172_002986"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO172_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5513101.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO172_002987"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO172_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5513102.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7800
                     /gene="iucD"
                     /locus_tag="OO172_002988"
     CDS             6484..7800
                     /gene="iucD"
                     /locus_tag="OO172_002988"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5513103.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7806..10001
                     /gene="iutA"
                     /locus_tag="OO172_002989"
     CDS             7806..10001
                     /gene="iutA"
                     /locus_tag="OO172_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5513104.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10552..10695)
                     /locus_tag="OO172_002990"
     CDS             complement(10552..10695)
                     /locus_tag="OO172_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513105.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10945..14832
                     /gene="sat"
                     /locus_tag="OO172_002991"
     CDS             10945..14832
                     /gene="sat"
                     /locus_tag="OO172_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCR5513106.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4004 a         3540 c         3696 g         3624 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatggctcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg tcgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagaa gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccc tgcttcttta actcactata cagcaaatat tcaggaataa
     7801 cgatgatgcg caaaaagtat atgccccggg ctcttggtcc gctgcttctt gtcgtgctgt
     7861 caccagctgt cgcccagcaa aacgatgata atgagatcat agtgtctgcc agccgcagca
     7921 atcgaactgt agcggagatg gcgcaaacca cctgggttat cgaaaatgcc gaactggagc
     7981 agcagattca gggcggtaaa gagctgaaag acgcactggc tcagttaatc cccggccttg
     8041 atgtcagcag ccaaagtcga accaactacg gtatgaacat gcgtggccgc ccgctggttg
     8101 tcctgattga cggtgtgcgc ctcaactctt cacgttccga cagccgacaa ctggactctg
     8161 tcgatccttt taatatcgac catattgaag tgatctccgg cgcgacggcc ctgtacggtg
     8221 gcgggagtac cggagggttg atcaacatcg tgaccaaaaa aggtcagccg gaaaccatga
     8281 tggagtttga ggctggcaca aaaagtggct ttaacagcag taaagatcac gatgagcgca
     8341 ttgccggtgc tgtctccggc ggaaatgacc atatctccgg acgtctttcc gtggcatatc
     8401 agaaatttgg cggctggttt gacggtaacg gcgatgccac cctgcttgat aacacccaga
     8461 ccggcctgca gcactccaat cggctggaca tcatgggaac cggtacgctg aacatcgatg
     8521 aatcccggca gcttcaactg ataacgcagt actataaaag tcagggagac gacaattacg
     8581 ggcttaatct cgggaaaggc ttttccgcca tcagcgggag cagcacacca tacgtcagta
     8641 aggggctgaa ttctgaccgc attcccggca ctgagcggca tttgatcagc ctgcagtact
     8701 ctgacagtga tttcctggga caggaactgg tcggtcaggt ttactaccgc gatgagtcgt
     8761 tgcggtacta cccgttcccg acggtaaatg cgaataaaca ggcgacggct ttctcctcgt
     8821 cacagcagga taccgaccag tacggcatga aactgactct gaacagccaa ctgatggacg
     8881 gctggcaaat cacctggggg ctggatgctg agcatgagcg ctttacctcc aaccagatgt
     8941 tcttcgatct ggctcaggca agtgcttccg gagggctgaa caaccataag atttacacca
     9001 ccgggcgcta tccgtcatat gacatcacca atctggcggc cttcctgcaa tccagctatg
     9061 acattaatga tatttttacc gttagcggtg gcgtacgcta tcagtatact gagaacaggg
     9121 tagatgattt catcgactac acgcagcaac agaagattgc tgccgggaag gcgatatctg
     9181 ccgacgccat tcctggtggt tcggtagatt acgataactt tctgttcaat gctggtctgc
     9241 tgatgcacat caccgaacgt cagcaggcat ggttcaattt ttcccagggg gtggcattgc
     9301 cggatccggg gaaatattat ggtcgcggca tctatggtgc agcagtgaac ggccatcttc
     9361 ccctgacaaa gagcgtgaac gtcagcgaca gtaagctgga aggcgtgaaa gtcgattctt
     9421 atgaactggg ctggcgcttt atcggtgaca acctgcggac tcaaatcgcg gcatattact
     9481 cgctttccaa taagagcgtg gaaaggaata aagatctgac catcagtgtg aaggacgaca
     9541 ggcgccgtat ttacggcgtg gaaggtgcgg tggactacct gatcccggat actgactgga
     9601 gtaccggtgt gaacttcaat gtgctgaaaa ccgagtcgaa agtgaacggt caatggcaaa
     9661 aatatgacgt gaaggaatca agtccatcga aagcgacagc ttacattaac tgggcgccgg
     9721 aaccgtggag tctgcgtgta cagagcacca cttctttcga cgtaagcgat gcagagggta
     9781 acgatattaa tggttacact accgtcgatt ttatcagtag ttggcagctt ccggtgggaa
     9841 cactcagctt cagcgttgag aacctcttcg accgtgacta taccactgtc tggggacagc
     9901 gtgcacctct gtactacagc ccgggttacg gccctgcttc actgtacgac tacaaaggcc
     9961 ggggccgaac ctttggtctg aactactcag tgctgttctg acggggatgc ttttgcaaca
    10021 agggtatgct gtaaacaaca tggtccttgg tggccatgtt gtttacagcc agtataatat
    10081 acagccttaa caagatattc cgtctccgga atgggctgtt caccactgaa taacattatg
    10141 tctgttttat ggatctttga ctgaaattta tcgcatatcc tcatttcatt gtccgtctga
    10201 aaactaacct tacaagtaag cgtcgtctca gcaccgtctg gcagatcctg aaattcctga
    10261 gaggatgatc tgcgcccaat atcctgagcc ggaactttgc tgcaatgaag gccaacgaaa
    10321 aatgggggag tatgttatag agtacttgct acagggtaaa aagaggtacc tgtcgctggt
    10381 tctccgttca gtttaaccgg gagattttct cctgcgcctg tcataaagtc cggtaatgga
    10441 gattgttaat acgatggtgc acgatgcatc gtcagtgccc ggcccggagg atgccccgta
    10501 gctacatacg gatcagggtt ggcaatatcg aatggcaggc tataggcaaa gttaaagccc
    10561 catggagtag cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc agtgatggag
    10621 aacttcttcg gcacactgca aaatcgcatc gtggtcgatc gctaattcgt cgtgaatcac
    10681 tgaacgatgt tccatgcgaa cagatattcg atcatatcga aatattaaac attacccctc
    10741 cccacattgg gcaaaaaaaa acaaaaaacg atcaattaac gatcaattag tattgattca
    10801 cataatctct catcattaat gagaacattg tcacatattt acaatataga actgttactt
    10861 ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata ctttgttcat
    10921 tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata tagtgctgcc
    10981 actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa aacaaaccga
    11041 aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc agcaaatatt
    11101 gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa aggtattttc
    11161 cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt ctctttccat
    11221 aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac cgcaatagga
    11281 ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc agaaactcag
    11341 gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga ttttgagatt
    11401 cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga gaccaaccct
    11461 acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg ttcaaaaaaa
    11521 atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga atccaaaatc
    11581 tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga ggtcacccaa
    11641 tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata
    11701 ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta
    11761 gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa
    11821 tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat
    11881 ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc
    11941 actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat
    12001 gttctgttta aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat
    12061 gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa
    12121 gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc
    12181 cccggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat
    12241 gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag
    12301 gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact
    12361 aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc
    12421 actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc
    12481 aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac
    12541 aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac
    12601 acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag
    12661 catgcaatat tcagaagcac aacgagtcat tgctccctgg tatttctttg tggaacggac
    12721 tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac
    12781 aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa
    12841 tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa
    12901 ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat
    12961 aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact
    13021 aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata
    13081 gccataggtg atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct
    13141 ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt
    13201 attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta
    13261 acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc
    13321 attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt
    13381 actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca
    13441 gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct
    13501 acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta
    13561 gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca
    13621 gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca
    13681 gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca accttctgtt agtcgacttc
    13741 attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc accaaaagga
    13801 actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga tgtaacaccg
    13861 gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg ctataaatct
    13921 gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg ctataaagcc
    13981 ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga cattaacggt
    14041 gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg tggattcagt
    14101 gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga tggccttgac
    14161 ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga tgccttcagt
    14221 ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga gtccggagca
    14281 tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc aactttcgcc
    14341 ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga agtcggttac
    14401 cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt ttacggtgct
    14461 gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat gaaggataag
    14521 gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt ctccggtaag
    14581 gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt tgccaacggt
    14641 gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa agacggtcgt
    14701 atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt cggtcttgag
    14761 tttgagaaat cggcatttgg taaatacaac gtggataacg cgatcaacgc caacttccgt
    14821 tactctttct gataacagcc cgggccgcgt ttgcggccct tctt
//
LOCUS       DAJTNM010000096        14724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTNM010000096 DAJTNM010000000
VERSION     DAJTNM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.096"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..581
                     /locus_tag="OO172_002992"
     CDS             <1..581
                     /locus_tag="OO172_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5513107.1"
                     /translation="IGRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCSDSSDVDN
                     DGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADVPKNMTQQNCQSKYENCYYDN
                     VEQSWIPVVSGFLLGRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRFGSGKKE
                     SYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            584..1747
                     /locus_tag="OO172_002993"
     CDS             584..1747
                     /locus_tag="OO172_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5513108.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1831..3455
                     /locus_tag="OO172_002994"
                     /pseudo
     CDS             1831..3455
                     /locus_tag="OO172_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3572..3847)
                     /gene="yjfN"
                     /locus_tag="OO172_002995"
     CDS             complement(3572..3847)
                     /gene="yjfN"
                     /locus_tag="OO172_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5513109.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3996..4325)
                     /gene="bsmA"
                     /locus_tag="OO172_002996"
     CDS             complement(3996..4325)
                     /gene="bsmA"
                     /locus_tag="OO172_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5513110.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4507..5256
                     /gene="yjfP"
                     /locus_tag="OO172_002997"
     CDS             4507..5256
                     /gene="yjfP"
                     /locus_tag="OO172_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5513111.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5253..6008)
                     /gene="ulaR"
                     /locus_tag="OO172_002998"
     CDS             complement(5253..6008)
                     /gene="ulaR"
                     /locus_tag="OO172_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5513112.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6116..7180)
                     /gene="ulaG"
                     /locus_tag="OO172_002999"
     CDS             complement(6116..7180)
                     /gene="ulaG"
                     /locus_tag="OO172_002999"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5513113.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7535..8932
                     /gene="ulaA"
                     /locus_tag="OO172_003000"
     CDS             7535..8932
                     /gene="ulaA"
                     /locus_tag="OO172_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5513114.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            8948..9253
                     /gene="ulaB"
                     /locus_tag="OO172_003001"
     CDS             8948..9253
                     /gene="ulaB"
                     /locus_tag="OO172_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5513115.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9263..9727
                     /gene="ulaC"
                     /locus_tag="OO172_003002"
                     /pseudo
     CDS             9263..9727
                     /gene="ulaC"
                     /locus_tag="OO172_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
     gene            9741..10391
                     /gene="ulaD"
                     /locus_tag="OO172_003003"
     CDS             9741..10391
                     /gene="ulaD"
                     /locus_tag="OO172_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5513116.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10401..11237
                     /gene="ulaE"
                     /locus_tag="OO172_003004"
     CDS             10401..11237
                     /gene="ulaE"
                     /locus_tag="OO172_003004"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5513117.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11237..11923
                     /gene="ulaF"
                     /locus_tag="OO172_003005"
     CDS             11237..11923
                     /gene="ulaF"
                     /locus_tag="OO172_003005"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5513118.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12052..12327)
                     /locus_tag="OO172_003006"
     CDS             complement(12052..12327)
                     /locus_tag="OO172_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5513119.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12654..13049
                     /gene="rpsF"
                     /locus_tag="OO172_003007"
     CDS             12654..13049
                     /gene="rpsF"
                     /locus_tag="OO172_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5513120.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13056..13370
                     /gene="priB"
                     /locus_tag="OO172_003008"
     CDS             13056..13370
                     /gene="priB"
                     /locus_tag="OO172_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5513121.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13375..13602
                     /gene="rpsR"
                     /locus_tag="OO172_003009"
     CDS             13375..13602
                     /gene="rpsR"
                     /locus_tag="OO172_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5513122.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13644..14093
                     /gene="rplI"
                     /locus_tag="OO172_003010"
     CDS             13644..14093
                     /gene="rplI"
                     /locus_tag="OO172_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5513123.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14164..>14724)
                     /locus_tag="OO172_003011"
     CDS             complement(14164..>14724)
                     /locus_tag="OO172_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5513124.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     RTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3427 a         3601 c         3973 g         3723 t
ORIGIN      
        1 gcattggtag accgaataat ccttttgaac cgcgtcgcaa tcgctacgca caaaaatact
       61 taacgttggc gctaatgggc ggtgccgctt ttttcgtatt gaaaggttgt agcgatagta
      121 gcgatgtcga taacgacggc gacggaacgt tttacgcgac agtgcaggat tgtattgatg
      181 acggtaataa tgccgacatt tgcgcgcgtg gctggaacaa cgccaaagcg gcattttatg
      241 ccgatgttcc aaagaatatg actcagcaga actgtcagtc taagtacgaa aattgctact
      301 acgacaatgt tgaacagagt tggatcccgg tcgtttctgg atttttgtta ggccgggtta
      361 ttcgtaaaga tcgcgatgag ccgttcgtct ataacagcgg cggatcctca tttgcttccc
      421 gccccgtctg gcgcaacact tccggtgatt actcctggcg cttcggttct ggcaaaaaag
      481 agtcttactc ttcaggcggc tttaccacca gaaaagcgtc gaccgtttcg cgcggcggct
      541 atggtcgttc ttccagcgcc cgtgggcatt ggggaggcta atcatgctga gacacaacgt
      601 tcctgtgcgg cgggatctgg accagatcgc cgctaacaac ggtttcgact ttcatatcat
      661 cgacaatgaa atctattggg acgagagtcg ggcttaccgt tttactctgc gccagattga
      721 agagcagatc gaaaaaccga ctgcggaact gcatcagatg tgccttgagg tggtggatcg
      781 cgcggttaaa gacgaagaga tcctgacgca actggcgatc ccgccgttgt actgggatgt
      841 gatcgccgaa agctggcgcg cacgcgatcc ttcgctgtat ggccggatgg attttgcctg
      901 gtgtggcaat gcgccggtga agctgctgga gtacaacgcc gatacgccga cctcgttgta
      961 tgagtcggct tatttccagt ggttgtggct ggaggatgcc cggcgcagcg gcattattcc
     1021 gcgtgatgcc gatcagtaca acgccattca ggaacgcctg atttcgcgct ttagtgagct
     1081 ttacagtcgg gaaccgtttt atttttgctg ctgtcaggac accgatgaag acaggagtac
     1141 cgtgctgtac ttgcaggact gcgcccagca ggcagggcag gagtcgcggt ttatctacat
     1201 tgaagatctc ggtttgggcg tcggcggcgt actgaccgat cttgatgata atgtcatcca
     1261 gcgtgcattt aagctgtatc cgctggagtg gatgatgcgt gacgataacg gtccgctgct
     1321 gcgcaagcgt cgcgagcaat gggtggagcc gttatggaaa agtatcttga gtaataaagg
     1381 gctaatgccg ctgctttggc gcttcttccc tggtcatcct aaccttcttg catcctggtt
     1441 tgagggtgaa aaatcgcaga ttgccgctgg cgaaagctat gtgcgtaaac cgatctactc
     1501 gcgcgaaggc ggaaacgtca ccattttcga cggtcagaat aacgtcgttg accacgctga
     1561 tggtgattac gccgatgaac cgatgatcta ccaggcgttt caacctctgc cgcgatttgg
     1621 cgatagctac acactcatcg gtagctggat tgtcgatgat gaagcgtgcg gaatggggat
     1681 ccgtgaagat aacacactga tcaccaaaga cacctcacgt ttcgttccgc attacattgc
     1741 tggataagaa tgttttagca atctctttct gtcatgaatc catggcagtg accatactaa
     1801 tggtgactgc cattgatgga gggagacaca gtgcactggc aaactcacac catttttaat
     1861 caacctatac cattaaataa cagcaactta tacctgtctg atggcgcgct ttgcgaagcg
     1921 gtaacgcgtg aaggtgctgg ctgggatagc aattttctag ccagtattgg tcagcagtta
     1981 ggaacggctg aatcccttga actggggcgg ctggcgaatg taaacccgcc tgaactgttg
     2041 cgctacgatg cccaggggcg gcgtctggac gatgttcgtt tccatcccgc atggcacctg
     2101 ctgatgcagg cgttatgtac caatcgggtg cacaatcttg cctgggaaga agacgctcgc
     2161 tccggcgcat ttgtggcgcg cgcggcgcgt tttatgttac atggcaggtt gaggctgggt
     2221 cgttatgtcc aataacgatg acctttgccg ccacgccatt gttgttacag atgttaccca
     2281 cgccgtttca ggactggacc acgccgctgc tgagcgatcg ctacgattct cacttattgc
     2341 caggtgggca aaaacgcggt ttgttgattg gcatgggaat gacggaaaag cagggcggtt
     2401 ccgatgtcat gagcaacacc atccgtgcag agcgtttgga agatggctct tatcggctgg
     2461 tggggcataa atggtttttc tcggtgccgc aaagcgatgc gcatctggtg ctggcgcaga
     2521 ccgcgggcgg tctgtcctgc ttttttgtgc cgcgcttttt gcctgacggg caacgcaacg
     2581 cgattcgcct cgagcggctg aaagataagc tgggtaatcg ctctaacgcc agttgcgaag
     2641 tggagtttca ggatgccatt ggctggttat tggggcagga aggggaagga attcgtctga
     2701 tcctgaaaat gggggggatg acgcgttttg attgcgccct gggtagccat gccatgatgc
     2761 gccgtgcatt ttcgctggcg atttatcatg cacatcaacg ccatgttttt ggtaatccat
     2821 tgatccaaca gccccttatg cgtcatgtct taagtcgcat ggcacttcag cttgaagggc
     2881 aaacggcgtt gctgtttcgt cttgcgcgag cgtgggaccg gcgtgccgat gccaaagaag
     2941 ccctgtgggc gcgtttattt acgcctgcgg caaaatttgt gatctgcaaa cgaggtatgc
     3001 cgtttgtggc cgaagcgatg gaggtgctgg gcggcattgg ttattgcgag gagagcgagc
     3061 tgccgcggct ttaccgggag atgccggtaa acagtatttg ggaaggttcc ggcaatatta
     3121 tgtgcctgga tgtgttgcgc gttctcaata agcaagcggg cgtatacgac ttattgtcgg
     3181 aagcatttgt ggaagtgaaa gggcaggatc gctattttga tcgcgcggtt cgtcgtttac
     3241 agcagcagct gcgtaagcca gctgaagaac tggggcgaga gattactcat cagctattcc
     3301 tgctgggctg cggtgcgcaa atgttgaaat atgcttctcc gccaatggcg caggcgtggt
     3361 gtcaggtgat gttagatacg cgcggcggcg tacggttgtc agagcagatc cagaatgatt
     3421 tattgctgcg ggcgacgggg ggagtgtgtg tgtaagcgta tacgactgat gcgacgctgg
     3481 tttcgattaa ctaaatgaaa tatgtgaaaa ttgtaggccg gacaaggcgc tcgcgccgca
     3541 tccggcattg ttcatttgct gtagatgaca ctcatgcata cagtatcgcc tgtacgcgcc
     3601 agttatcagg ctgctggtct tcgtacatgg taataatgcg gtaccatgac gcaccttgtt
     3661 catcggcctt taacgccacg atacgttcca cgtcctgcgg atcccctgaa atattgctaa
     3721 ctgagatctg acctatttca ttcaggcccg tcacgcagtc agcactggcg aattctgcag
     3781 actgtgccgt ggcgctcgca ggataagtca ctaattgcag cgagggtgag gcaagtaatt
     3841 gtttcatcgt cagctccatt tttcgtttcc ccacatgttg cagggcattc catcgcggta
     3901 ataaacgccc aagcttccag ccggtaattt attgtgcaca ggtaaacgca tagggacgca
     3961 aagcaatgta aagctgacta tgtaagttgt ctgatttatt tacggtacaa aatggcttgt
     4021 gaataccact gtcctgtcac gatggtttcg tccaccatga cgacaacgta ataatcagct
     4081 tttgcagcga cagcttttgc tctgatttct gctaatgcgt catccggaga accccgaacc
     4141 atcgtgctta cgctacctat tcgctgtaac ccttgcgtct ggtcgcggcg aatctcttgc
     4201 ggatggtccg ttactggcgg tgctggctgt ggcgtacctt gcagtgcgct acaggcactt
     4261 aacatcaaca ccaataataa actggcaaac cggtaaataa cgctattacg tttcctgcta
     4321 accatagtgt agtgccttat taatttaact ttgggggaat gttcccgaat tgttacctgg
     4381 tacgcgattt tcgaatgaaa acgtgacgaa agcgtaaccc acatctcaac attatgaact
     4441 aataccgcca gcagaaactt gtgctaagga tttcttcatg tcataccaga caaaaaggag
     4501 agacagatga ttgaaataga atcacgcgag ctggcagata ttcccgttct tcatgcttat
     4561 cctgtcgggc aaaaagatac cccgttaccg tgcgtaattt tttatcacgg ctttacttca
     4621 tccagcctgg tgtatagcta ttttgccgtt gcgctggcgc aggctggttt gcgggtgatc
     4681 atgccggatg cgcccgatca cggtagccgt tttagtggcg acgaagcgcg gcggttaaat
     4741 caattctggc aaatcttgct acaaagtatg caggaattca ctactttacg tgcggcaata
     4801 gccgaagaaa actggctgct tgatgaccgt ctggcagtcg gtggcgcgtc gatgggcgcg
     4861 atgacggcac tgggaattac cgctcgccac cccacggtga aatgtaccgc cagcatgatg
     4921 ggatcgggct attttacatc gctcgcccgt tcactgtttc caccgctgat acctgaaacg
     4981 gcagcacagc agaatgaatt caataaaatt gtcgcgccgc tggcagagtg ggaagcgaca
     5041 aaccacctag aacaacttgg ggacagacct ctactgctgt ggcatggcct cgacgatgac
     5101 gttgtgcctg ccgacgaatc actacgtttg cagcaggcct taagcgagac gggacgggat
     5161 aaactgctaa cctgttcatg gcagccaggc gtgcgtcacc gcattacgcc tgaggcgtta
     5221 gatgctgccg tgacattttt ccgccagcat ctttaaacac gcagaatgct gaccccttgc
     5281 gcttccagtt gttgcaggat ttccgggtta gcatttttgc cggtgatcag catatcgatt
     5341 tgatcggcac ggctaaaaag cattcccgcg cgttcgccaa tcttactgct atcaaccagt
     5401 accaccagtt tccctaccac gctcagcatc ttctgctctg ccattgctgt cagcatatcg
     5461 gttttataca acccttctgc ggtcagccct tttccgctgg taaacatcca gtgcccggca
     5521 tagagactgt tttcgctgcc ctgcgggctt aaagtgatgg actgactttt gttgtactgt
     5581 ccacccataa tgatcacgct gtcatgttct tgatcgatca ggtaatttgc cagcggtaga
     5641 taattagtga tgatttgcac tggcttgcca cacatttccc gcccaagcag aaacgcggtg
     5701 gagccgcagt tgatgactac gctttcgccc ggattaacca gctgcgaggc cgctttagca
     5761 atacgtactt tttcatcgtg attctgcgcc tgatgcagat tcatcggcgt ccagcgcggg
     5821 cgctgttggg taatagcttc tgcgccattg cgcacctttt tcagtttgcc gctttcgcca
     5881 agtttattga tatcgcgtcg cgcagtggca ggcgaaattc ccagacgctc aacgactttc
     5941 tcaacggtca caaagcccaa ttgtgcgagc atttccagga ggatttgatg tctttgtgct
     6001 tcagtcatga gctattccga taaaatttga tttttttaga tgatatttga aatagccagg
     6061 aaatactacg ccggatagcg cgaacttctc cacgctatcc ggtataaacg acagattaca
     6121 ggaatgactt gaacggcaga tccggttcaa tagtgaagca atcatcgaaa ccgcgcggat
     6181 agtggtactc gaagttgtct ttatccagcg gccaggtaaa tttgccgcca acctgccaga
     6241 taaacggctt gaagccatac ttcagacgat cttttttcat ctcccacagc acgcggatct
     6301 cttgcggatc ggcctggaag tttgaccaga tgtcgtggtg gaacgggata accactttcg
     6361 cattcagggc ttcacccata cgcagcatat cggcgctggt cattttgtcg gtaataccgc
     6421 gcgggttctc accatacgat cctaacgcca catcgatctg atgttcgtta ccgtgcttcg
     6481 cataatagtt agagtagtgg gaatcgccac tgtgatatag ggaaccgcca ggggttttga
     6541 acaggtagtt tactgcgcga tcgtccatgc catctggcag tacgccagcc gctttttgat
     6601 cggcaggcag ggtgatcagt gcagtacggt cgaaagcgtc aagcgcatga atttcaatgt
     6661 ctttcacttt tacaacatcg cccggtttga ccacgatgca acgctctttc ggtacgcccc
     6721 agccaatcca caaatcaacg caggttttcg gtccaatgaa tggtacgtca tcagcacagt
     6781 tctgcatcac ggcagcagcg acgttaacgt cgatatgatc gttgtgatcg tgagtcgcca
     6841 gtaccgcgtc gatctggcga atcgcaaacg gatcaagaac aaacggggtg gtgcgtaggt
     6901 ttggctgcag ttttttcacg ccagccatgc gctgcatctg gtgaccctgt ttcattaacg
     6961 ggttaccgtg actttgtttg ccagtgccgc accagaaatc aacgcaaacg ttggtgccac
     7021 cttctgattt caaccagatc ccggtgcagc ctagccacca catcgcaaat gtgccagggg
     7081 cgacctgctc ttgttcaatt tcttcattca gccagctacc ccactccggg aaagtgctca
     7141 ggatccagga ttctcgggtg atacttttca ctttactcat cgccatttac cttcatgata
     7201 gttcaattcg aatcaatatg tgattggttt tgattaatcc tgacactatt ttttcaggaa
     7261 ggcaatgacc attttttgac tttttgccag ggaagttgtt gttgattttg agtgtagaaa
     7321 gatttaatgg aatgtatagt taaattaact aaatgaattt aaataaataa ttttttcgtt
     7381 gtgtgaatcc cactctatcc gtgtggaatt atttgcgggt cgcgtcacat ttaatcataa
     7441 ataatcttat tgtgattact tttgaaaatt agagtgagtg cacaacattc cgggtgtgcg
     7501 gaatacccgg ttacctcttc ttcaggagat cgttatggag atcctctaca acatctttac
     7561 cgtgtttttt aaccaggtca tgaccaatgc cccgttgttg ctgggtattg tgacctgtct
     7621 gggctacatc ctactgcgca aaagtgtcag cgttattatt aaaggcacga ttaaaaccat
     7681 aattggtttc atgttgttgc aggcagggtc cggcatcctc accagcacct tcaaaccggt
     7741 ggtggcgaaa atgtccgaag tctacggcat taacggcgca atttccgata cttacgcgtc
     7801 gatgatggca accatcgacc gcatgggcga tgcctatagc tgggtaggtt acgcggtgtt
     7861 gttagcgctg gcgctgaaca tctgttacgt gctgctgcgt cgcattaccg gtattcgtac
     7921 catcatgctg actggtcata tcatgttcca gcaggcgggg ctgattgccg ttacgttgtt
     7981 tattttcggc tactccatgt ggacaaccat tatctgcacg gcgattctgg tttcgctcta
     8041 ctggggcatt acctccaaca tgatgtacaa gccgactcag gaagtgacgg acggctgcgg
     8101 tttctccatc ggtcaccagc aacagtttgc atcattaatt gcctataaag ttgcgccgtt
     8161 cctcggcaaa aaagaggaaa gcgttgaaga cctcaaactg ccaggctggc tgaatatttt
     8221 ccacgacaac atcgtctcca cggcgattgt catgaccatc ttctttggtg ccattctgct
     8281 ctccttcggt atcgacaccg tgcaggcgat ggcaggcaaa gtgcactgga cggtctatat
     8341 cctgcaaacc ggtttttcct tcgcggtggc gatcttcatc atcactcagg gtgtgcgcat
     8401 gtttgtggcg gaactctctg aagcgtttaa cggtatctcc cagcgcctga tccctggtgc
     8461 ggttctggcg attgactgtg cggctattta tagcttcgct ccgaacgccg tggtctgggg
     8521 ctttatgtgg ggcaccatcg gtcagctgat tgcggttggc atcctggtcg cctgcggttc
     8581 ctcgatcctg attatcccag gctttatccc gatgttcttc tccaacgcca ccatcggcgt
     8641 gttcgctaac cacttcggcg gctggcgtgc ggcgctgaag atttgtctgg tgatggggat
     8701 gatcgaaatc tttggttgcg tctgggtggt gaaacttacc ggtatgagtg cctggatggg
     8761 gatggcggac tggtcgattc tggcaccgcc gatgatgcag ggcttcttct ccatcggtat
     8821 cgcctttatg gccgtcatca ttgtaattgc actggcttat atgttcttcg ctggccgcgc
     8881 actgcgcgca gaagaagatg cagaaaaaca attggcagaa cagtctgctt aataaggagt
     8941 tttgattatg accgtacgta ttctggctgt gtgtggcaac ggacaaggca gttccatgat
     9001 catgaagatg aaagtggacc agtttttaac ccaatcaaac attgaccaca cggtaaatag
     9061 ctgcgcggtt ggcgaataca aaagcgagtt gagcggcgcg gatatcatca tcgcttctac
     9121 gcacattgcg ggcgaaatca ccgtgaccgg caacaaatac gtggttggcg tgcgcaacat
     9181 gctctctcct gccgactttg gcccgaaact gctggaagtg atcaaagcgc atttcccgca
     9241 ggatgtgaag taaggacgcg ccatgaaatt acatgattcg ctggcggaga ataaatccat
     9301 ccgcctgcag gctgaagctg agacatggca ggacgcagta aaaatcggcg ttgacctgct
     9361 ggtggcggcg gatgttgtcg agccgcgtta ctaccaggcg attctggatg cggttgaaca
     9421 gcacggtccg tactttgtgc tcgcaccagg cctggcaatg ccgcacggtc gcccggaaga
     9481 gggcgttaag aaaaccggtt ttgcgctggt gacgctgaaa aagccgctgg agttcaacca
     9541 cgaagataac gacccggtgg atatcctcat caccatggcg gtggtcgatg ccaacacgca
     9601 ctaggaagtg ggcatcatgc agatcgtcaa cctgtttgaa gatgaagaaa atttcgaccg
     9661 tttacgtgcc tgccgtaccg agcaggaagt actggatctc attgaccgca ccaacgcggc
     9721 agcttaagaa ggaattgaag atgtcattac cgatgttgca agtcgcgctg gacaaccaga
     9781 ctatggatag cgcctacgaa accactcgcc tgattgccga agaagtcgac attatcgaag
     9841 tgggtaccat tctgtgcgtg ggcgaaggcg tgcgtgcggt tcgcgatctg aaagcgctct
     9901 acccgcacaa aatcgtgctg gcagacgcca aaattgccga tgcgggcaaa attctttctc
     9961 gtatgtgctt cgaagccaac gctgactggg tgaccgtgat ttgttgtgcg gatatcaaca
    10021 ccgccaaagg tgcgctggac gtagcaaaag agtttaacgg cgacgtgcag atcgaactga
    10081 ccggttactg gacctgggaa caggcgcaac agtggcgcga cgcaggtatt cagcaggtgg
    10141 tttatcaccg cagccgtgac gcgcaggccg ctggcgtggc gtggggtgaa gccgatatca
    10201 ctgcgatcaa acgtctttcc gatatgggct tcaaagtcac cgtcaccggt ggcctggcgc
    10261 tggaagatct gccgctgttc aaaggcattc caattcatgt gtttatcgcc ggtcgcagca
    10321 tccgtgatgc cgcttctccg gttgaagccg cgcgtcagtt caaacgttcc atcgccgaac
    10381 tgtggggcta aggagcggat atgttgtcca aacaaatccc gcttggcatc tatgaaaaag
    10441 cgctccccgc cggggagtgc tggctggaac gcctgcgact ggcaaaaacg ttgggcttcg
    10501 attttgtcga gatgtcggta gatgaaactg acgagcgcct gtcgcgcctc gactggagcc
    10561 gcgagcagcg tctggcgctg gtgaatgcga ttgttgaaac cggcgtgcgt gtgccgtcca
    10621 tgtgcctttc tgcccatcgt cgtttcccgt tgggcagcga agatgacgcg gtgcgggcgc
    10681 aggggctgga gattatgcgt aaagccatcc agttcgccca ggacgttggc attcgcgtga
    10741 tccagctggc gggctatgac gtttactatc aggaagccaa taacgaaacg cgtcgtcgtt
    10801 tccgtgacgg cctgaaagag agcgttgaga tggcgagccg cgcacaggtg acgctggcga
    10861 tggagatcat ggattatccg ttgatgaact ccatcagcaa ggcgctgggt tacgcgcact
    10921 atctcaacaa cccgtggttc cagctctacc cggatatcgg caacctgtcg gcgtgggaca
    10981 acgatgtgca gatggagttg caggccggaa tcgggcatat cgtcgcggtg catgtgaaag
    11041 acaccaaacc gggcgtcttc aaaaacgtgc catttggcga aggtgtggtg gatttcgaac
    11101 gttgcttcga aacgctcaaa cagagtggct attgcgggcc gtacctgatt gagatgtgga
    11161 gcgaaacggc ggaagacccg gcggcagaag tggtgaaagc gcgcatggcc aaagcaggca
    11221 tggtggaggc ggcataatgc aaaagctaaa acagcaggtg tttgaagcca acatggatct
    11281 gccgcgctat gggctggtga cctttacttg gggcaacgtc agcgctatcg accgcgaacg
    11341 cgggctggtg gtgatcaagc ccagcggcgt tgcctacgaa accatgaaag cagacgatat
    11401 ggtggtggtc gatatgagcg gcaaggtggt ggaaggggag tatcgcccat cttccgacac
    11461 tgcgacgcat ctcgaactct accgtcgtta cccgtcgctt ggcggcattg tccataccca
    11521 ctccactcat gccaccgcat gggcgcaggc ggggctggcg atcccggcat taggcaccac
    11581 gcacgctgac tacttctttg gtgatatccc gtgtacgcgt ggattaagcg aagaagaggt
    11641 gcagggtgag tatgaactga acactggcaa agtgattatc gaaacgctgg gcaacgccga
    11701 gccgctgcat acgccgggaa ttgtggtgta tcagcacgga ccgttcgcct gggggaaaga
    11761 tgcccacgac gcggtgcata acgcggtagt gatggaagaa gtggcgaaaa tggcgtggat
    11821 tgcgcgcagt attaatccac aactcaatca tatcgacagc tacctgatga ataaacactt
    11881 catgcgtaag cacggtccta acgcttatta cgggcagaag taaaacacgc gctgcggaaa
    11941 tttccttcct cgggagataa ctggtctaat tccgcagccg tttttcaaaa aaagccccct
    12001 gcgaaggggg caaagcaaac tatggcaatg tttcgttggt tatacctggt gctagcgata
    12061 aatatccgcg ctggcgtgca tattgccgtt actccccggt tcccgcatca gaattacgtg
    12121 gtagtacgtt gcgccttgcg catctgtttc ttcatttaat gcctgacgtg cttcgctttc
    12181 agtggcgaaa ttatgattga tataaatcac gcctaagctt tgcacatcgt ccatatttct
    12241 ggcctggtgg ttattaattt caatggctgc ccatgtattt gcacttagca aaagcacagc
    12301 cagaagggct aaaactcgac tgaacataga tacctcctcg acggctgact ttgtgtgctc
    12361 tcctctgtga tgatcttctg atttaatttt aatcaatgat aaagaagttg atggtgacca
    12421 tttctgatgc agttgttcaa aaaaacactg tgacggaatg tgatgaactt caaatcagcg
    12481 gtttagaggt tatttgcgaa aggggagatt tatttctgct ccgcccttga gtttagcggg
    12541 gtatgtcagt actataacgc gtcatttttc agccgacctt taacacgttc cttgcctccc
    12601 cgggattcgg ctgacccaga caggaggctg aataatccgt aaggagcaat tcgatgcgtc
    12661 attacgaaat cgtttttatg gtccatcctg accagagcga acaggttccg ggcatgatcg
    12721 agcgctacac tgctgccatc actggtgcag aaggcaagat ccaccgtctg gaagactggg
    12781 gccgccgtca gctggcttac ccgatcaaca aactgcacaa agcacactac gttctgatga
    12841 acattgaagc tccgcaggaa gtgatcgatg agctggaaac taccttccgc ttcaacgatg
    12901 ccgttatccg cagcatggtt atgcgtacca agcacgctgt taccgaagca tctccgatgg
    12961 ttaaagcgaa agacgagcgc cgtgagcgtc gcgatgattt cgcaaacgaa accgctgatg
    13021 atgctgaagc tggggattct gaagagtaat ttctgatgac caaccgtctg gtgttgtccg
    13081 gcaccgtgtg caggactccc cttcgaaagg tcagtccatc aggaattcct cactgccagt
    13141 tcgtgcttga gcatcgttct gtgcaggagg aagccggctt tcaccggcag gcatggtgtc
    13201 aaatgcccgt tattgttagc ggacacgaaa accaggccat tactcacagt ataacggtcg
    13261 gcagtcgcat aaccgttcag gggttcattt catgccacaa ggcaaagaac ggactgagca
    13321 aaatggtttt gcacgccgag cagattgaat tgatagattc tggagactag ccatatggca
    13381 cgttatttcc gtcgtcgcaa gttctgccgt ttcaccgcgg aaggcgttca agagatcgac
    13441 tataaagata tcgctacgct gaaaaactac atcaccgaaa gcggtaagat tgtcccaagc
    13501 cgtatcaccg gtacccgtgc aaaataccag cgtcagctgg ctcgcgctat caaacgcgct
    13561 cgctacctgt ccctgctgcc gtacactgat cgccatcagt aatcggtcac ggtccattaa
    13621 tacgactttg agaggataag gtaatgcaag ttattctgct tgataaagta gcaaacctgg
    13681 gtagcctggg tgatcaggta aacgttaaag cgggctatgc tcgtaacttc ctggtaccgc
    13741 agggtaaagc tgttccagct accaagaaaa acattgaatt cttcgaagca cgtcgcgctg
    13801 aactggaagc taaactggct gaagttctgg cagctgctaa tgctcgcgct gagaaaatca
    13861 atgcactgga aactgttacc atcgcgtcta aagctggcga cgaaggtaaa ctgttcggtt
    13921 ccatcggtac tcgcgacatc gctgacgctg taactgcagc tggcgttgaa gtggctaaga
    13981 gcgaagttcg tctgccgaac ggcgttctgc gtaccactgg cgaacacgaa gtgagcttcc
    14041 aggttcacag cgaagtattc gcgaaagtga tcgtaaacgt agtagctgaa taattcgtta
    14101 ttcaacgaga cgtaaaaacg ccgaccattg gtcggcgttt tgctttctat ttttcgtcag
    14161 gcattaattt cgcaagtaga tccattcacc attctcttgt gtccataagt caggatggtt
    14221 tgctggcgtt agatgcatac gatcactcct tatgtctcgg tcctgcggca ctcgctcgaa
    14281 acctaaaccc gagtagattg cggctgaatt tgataaggaa cgtagaccaa ttttcaaatc
    14341 atggggtaaa tcagaatacg cgtttagcaa tgcagcaatg atgaatccga gggcattaga
    14401 aacgcctgta acagtgcgaa gttggtcgat ataaagatcg attgttggta gttcagatga
    14461 ccagcaatta ggcatttggg caggaagggt tacaacgtat cctatcgttt ggccggtata
    14521 gtctggagta ctataaccag gtcccaaaac aacctcgtgt attgtgtatt tactgttggg
    14581 ttcgaaggat tcgacatctt gcaataaata tttcagttct gcggttaacc tcgcacccgc
    14641 gtaagcttta ttgtatttct gggcgtgaat attatctaat gtagcttcat ggttccatcc
    14701 aaaagaatta tcaccattca ttgt
//
LOCUS       DAJTNM010000097        14678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTNM010000097 DAJTNM010000000
VERSION     DAJTNM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.097"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            146..1186
                     /locus_tag="OO172_003012"
     CDS             146..1186
                     /locus_tag="OO172_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5513125.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1219..2412)
                     /gene="lplT"
                     /locus_tag="OO172_003013"
     CDS             complement(1219..2412)
                     /gene="lplT"
                     /locus_tag="OO172_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5513126.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2405..4564)
                     /gene="aas"
                     /locus_tag="OO172_003014"
     CDS             complement(2405..4564)
                     /gene="aas"
                     /locus_tag="OO172_003014"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5513127.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            4953..5984
                     /gene="galR"
                     /locus_tag="OO172_003015"
     CDS             4953..5984
                     /gene="galR"
                     /locus_tag="OO172_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5513128.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(5991..7253)
                     /gene="lysA"
                     /locus_tag="OO172_003016"
     CDS             complement(5991..7253)
                     /gene="lysA"
                     /locus_tag="OO172_003016"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5513129.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7375..8310
                     /gene="lysR"
                     /locus_tag="OO172_003017"
     CDS             7375..8310
                     /gene="lysR"
                     /locus_tag="OO172_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5513130.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8297..8989)
                     /gene="ygeA"
                     /locus_tag="OO172_003018"
     CDS             complement(8297..8989)
                     /gene="ygeA"
                     /locus_tag="OO172_003018"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5513131.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAS
                     IHAEDAVAFMLS"
     gene            complement(9118..10506)
                     /gene="araE"
                     /locus_tag="OO172_003019"
                     /pseudo
     CDS             complement(9118..10506)
                     /gene="araE"
                     /locus_tag="OO172_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(10821..11582)
                     /gene="kduD"
                     /locus_tag="OO172_003020"
     CDS             complement(10821..11582)
                     /gene="kduD"
                     /locus_tag="OO172_003020"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5513132.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11612..12448)
                     /gene="kduI"
                     /locus_tag="OO172_003021"
     CDS             complement(11612..12448)
                     /gene="kduI"
                     /locus_tag="OO172_003021"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5513133.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(12768..13916)
                     /locus_tag="OO172_003022"
     CDS             complement(12768..13916)
                     /locus_tag="OO172_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5513134.1"
                     /translation="MKDVVVVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            14171..14678
                     /locus_tag="OO172_003023"
                     /pseudo
     CDS             14171..14678
                     /locus_tag="OO172_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT         3799 a         3977 c         3469 g         3433 t
ORIGIN      
        1 ttaaccgtga tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct
       61 ggccttaaga tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg
      121 tcgtgattat aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga
      181 agtcagcacg ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc
      241 ccacgcacaa ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat
      301 gtacccagta cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg
      361 gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc
      421 gcgcaataat gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga
      481 agcgctgcat gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca
      541 ctggccgcag cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc
      601 gcctgcggtt tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa
      661 aattcgttat atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct
      721 ggcggacaaa cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa
      781 ccgcagtttt gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc
      841 ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc
      901 tggcgcacgt aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa
      961 agccgtcgcg gcgtatgttg atatcgccag acgtcatggc cttgatcctg ctcagatggc
     1021 gctcgcgttt gttcgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat
     1081 ggatcagctg aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc
     1141 tgaaattgaa gcggtacatc aggtttatac ttatcctgca ccataaagat taacgccggt
     1201 taaaaccggc gttaaatatt aatgacggcg ctgccagatc cacagcgccg ttattgccag
     1261 cgcaaatagc gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc
     1321 cagcgagtaa atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc
     1381 aatcgcattc cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg
     1441 aacgacaaaa aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcagcag
     1501 ctcgtgttgc agggagaaaa tcagtaccac cacgccaatc aaaatcccgg ctggcataca
     1561 gcgtgacacg gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc
     1621 aatcgctacc atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac
     1681 cggtacccac agcaccaaca ggaaacgcag cgtgacaccc gctccccaga ataaactggt
     1741 gcccaccagc gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg cattcaggaa
     1801 actgcgggtc atgttgatga gattccagga ctgccccgga cgcgccgccg ccagtttggg
     1861 aatgtagata ttggcaacga ccgcaccacc gtaggccagt gcgcatgcgg ccagggcgac
     1921 caggacatgc cagtcagcca gcacaccacc ggctacggaa ccgagtaaaa tcgccgctat
     1981 ggcagaagct tccattaaac cgttagcttt tactaactta ctacccgtgg ttaattcgcc
     2041 gagaataccg tatttcgccg gtgaataggc tgcagcacca acacccacca gcgtatagcc
     2101 gagaaacgga ttgataccaa agcagatact ggctgcgccc agcagcttca ggccgttggc
     2161 aaacatcatc acccggcctt tggcgaagct atccgccacc tgcccgacaa acggcgccaa
     2221 aagaatgtaa gcacctacaa acaccatttg caggatgggc tggctccact ccggatagaa
     2281 ctgcgctttc agtaacgcca gagtggcaaa cagtagcgcg ttatcgccaa acgcagagag
     2341 aaactgcgcc acgataaccg ctttcatccc cttcgaccac aacgaagtgt tagtgtgcac
     2401 tgactcactc atcgtgttgt tccgcttcgt ctacccagct tttcaacgtg acaaagtcag
     2461 gtttgccgct gccaagtaat ggcatctgtt tcagatagcg aatatcgcgc ggtacagcaa
     2521 gctccggcac gccgtgctcg cgggcatact gttgcaactt atcgcgcgtc agttcgttat
     2581 ctgtggtgaa aagcaccagt gcctcgcctt tgctggcatc gctcttaatc gcagtggcat
     2641 ggactttatc tggcgaaaca ccaagtgcca gttgttccac catttccagc gacaccattt
     2701 cgcctgcaat tttggcaaag cgttttgcgc ggccctgaat ctgcacaaag ccctgctcgt
     2761 caaaacgcac aatatcgcca gtgtcatacc agccgcgctc catttcgccg cgaacattct
     2821 cggcggtggg cacttccagt acacctggct tctccacccg cagatagccg ttcattatgt
     2881 tcggcccttt cagttgcagg cgtccgccct cttcgatacc agggaccgac aacaggcgtg
     2941 catccattcc tggtagaata cgccctaccg taccgggttt cgccgccatc ggtacgttga
     3001 tagaaacgac aggcgcgcat tcggtcacac cgtagccttc aaggatgcgc aggccaaatt
     3061 tatcctgcca aagctgtttg gtactttctt gtagtttttc tgcgcctgcc accacatagc
     3121 gtagacgata gaagtcatac gggttggcga aacgcgcgta gtgaccgagg aaagtcgagg
     3181 taccgaacaa cacggtgcaa ctgcggtcat acaccaagtc cggcacaatg cggtaatgca
     3241 gcgggcttgg ataaaggaac acttctgcac ctgtaagcag tggcgtaaac aggcctaccg
     3301 tcagcccaaa ggagtgaaac agcggtaatg ccgacataaa gcgatcgttg gtggtgaagt
     3361 cggcaatcgt tttaatctgc tcgacgttcg ccagaatgct tttatggcta tggacgacgc
     3421 cttttggatg gccttcagaa ccggaggtaa agaggatcag cgcctcttct tccggctgct
     3481 gttttacctg cgccaggcgc ggcatcagca aatgagcgaa gatccatact ttgtcggcag
     3541 tggtgacatc tgcttttaaa tcttccagat agacccagcg cacctgagta agttgctccg
     3601 gcagatgcca gagtttgcct ttatcgagaa actggcggga agtgaagatg gttttgattt
     3661 cagccgccgt aatagcactg gtcagccctt ttaccccggc agtgtagttc atcattgcgg
     3721 gaatgcggcg acgcgcgatg gccccaaaaa tcactgccgc actgatgcct gcattgggca
     3781 gcattaagcc gatgcgttcg ccttcaacac tgtatttttc aaggatgcgt ccaacaaaca
     3841 gcgttttcgt aagcaatttg cgataggagt ctggggtaaa gttgacgtct tcgacacatt
     3901 tcttcccggc tccgaagcgg tacattgcac tcagtaaaga ttcgtacaga gtttcacgcg
     3961 ggcgcaccgc catacgcgct tccatcatta tttgatgcag catttcgcca gcgattttgc
     4021 gacggtcacg ggcacgcggc gcatccggca tcgccacctg cgttggaggc aaaatatgca
     4081 gagtaatttg cgggaacaag cgacgtttaa ccagaccttt caggcggctg aagtgcgtaa
     4141 gttccgcccc ttcaatacgc acaggaataa ccgttgcacc agacttcgcc gcgacaaaac
     4201 ccgcgccatc gtagattttc atcagcgagc ctgtagtggt gatgcgtcct tcagggaaaa
     4261 tcaccactgg tcggccctgt tccaccagac gtaccagatg tttaatagcc ataggttgcg
     4321 tcgggtcgag aggaacaaag tcgataaatg atttcagcca acgcatatac cactgttggc
     4381 ttattgaggt gtaaacggca aacactggac gcacaggtaa aaacagtcca agcaaaatgc
     4441 catcaataaa agagacgtga ttaggcgtaa ttagaacgcg ctcgcccttc agtgcctggg
     4501 tgtcacccgt aacgcgaacg cgatacaaaa cacggcacaa atttcgaaaa aagctaaaaa
     4561 gcattccaac tccctttgct ctgattcagt aaaagcgaat ggagggagat tacacgagat
     4621 aaagaacgcg agcgacagta aattaggtgc gaaaaaaaac ctgcgcatcc gcgcaggttg
     4681 gtgcaagaga cagggtacga agagcgtacc gaataatctc accaatcaat acctctggga
     4741 tcaccacttt agcaacctga agccaaacgc caccagcggt caatcgcaac agcgtttcgc
     4801 aaagtgtaac caaaggtttg caatctcttt ttttgtctta cttatttgcg tttggctcac
     4861 attcccacga tgaaaacacg ccaccccttg aaccaacggg cgttttccgt aacactgaaa
     4921 caatgtaagc gtttacccac taaggtattt tcatggcgac cataaaggat gtagcccgac
     4981 tggcaggcgt ttcagtcgcc accgtttccc gcgtcattaa taattcaccc aaagccagcg
     5041 aagcttcccg gctggctgtg catagtgcaa tggagtctct tagctatcac ccgaacgcca
     5101 acgcccgtgc gctggcgcag cagaccactg aaacggtcgg tctgatcgtt ggtgatgttt
     5161 ccgatccgtt tttcggtgca atggtgaaag cggtcgaaca ggtggcttat cacaccggta
     5221 atttcttatt gattggcaac ggttaccaca acgaacaaaa agagcgtcag gctattgagc
     5281 aactgatccg ccatcgctgt gctgcgttgg tcgtccatgc caaaatgatc ccggatgccg
     5341 atttagcctc attaatgaaa caaatgcccg gtatggtgct gatcaaccgt atcctgcctg
     5401 gctttgaaaa ccgttgtatt gctcttgacg atcgttacgg tgcctggctg gcaacgcgtc
     5461 atttaattca gcaaggtcat acccgcattg gttatctgtg ctctaaccac tctatttctg
     5521 acgccgaaga tcgtctgcaa gggtattacg atgcccttgc tgaaagtggt attccggcca
     5581 atgatcggct ggtgacattt ggcgaaccag acgaaaacgg cggcgaacag gcaatgaccg
     5641 agcttttggg acgaggaaga aatttcactg cggtagcctg ttataacgat tcaatggcgg
     5701 cgggcgcgat gggcgttctc aatgataatg gtattgatgt accgggtgag atttcgttaa
     5761 ttggctttga tgatgtgctg gtgtcacgct atgtgcgtcc gcgcctgacc accgtgcgtt
     5821 acccaatcgt aacgatggcg acccaggctg ccgaactggc tttggcactg gcggataatc
     5881 gccctctccc ggaaattact aatgtattta gtccgacgct ggtacgtcgt cattcagtgt
     5941 caactccgtc gctggaggca agtcatcatg caaccagcga ctaaccgcag ttaaagcaat
     6001 tccagcgcca gtaattcttc gatggtctgg cgacggcgaa tcaaccgcgc ctgaccatta
     6061 tcaaacagaa cttctggtaa cagcggacgg ctattgtagt tggatgacat tgatgcgcca
     6121 tatgcccctg tatcatgcag taccagataa tcacctgcct tcacttccgg caaggcgcgg
     6181 gtttcaacat ttcccccttc ctgctgggta aagacatcgc ccgattcaca taacggcccg
     6241 gcgacgacgg tttccaccgt tggcgcgtgt tccagagaac gaccatcagc tgccagggca
     6301 ctgatatggt ggtaactacc gtacattgcc gggcgcatca gatcgttgaa cccggcatca
     6361 accagcacaa agtggcggct acccatttgt ttgacgctcc gcacctgagt aattaatacg
     6421 ccagactgcg ctaccaggaa gcgacccggt tcaatttcca gtttcacagg gtggcccaaa
     6481 tggcgagcga tttgctcacg cgcggcattc cacagaccat aataatgttc ggtatcaacc
     6541 gcctcttcac cctgttgata aggaatagaa agcccaccgc ccgcagaaat agcctgtaaa
     6601 tcctgaccga attcgatgac ctgacgcacc atagcaccac acacctgctc cagatgggca
     6661 taatcaacgc cagaaccaat gtgcatgtga atgccgacta gctgcagatg atgacgttgt
     6721 atcacgtcca gtgcggcggg cagatcggtg taccagatac cgtgcttgct gttttcgcca
     6781 ccggtattgg ttttttggct atgtccgtga ccaaaccccg gattaacgcg cagccatacc
     6841 cgatgccctg gcgaaacctg gcccagttgg tcgagcatat caacagaacc cgcattcacc
     6901 ggaatttgca attcactgac gcgttcaagc gtcgcctgat cgataacatc tgccgtaaaa
     6961 acaatatcat cggggtgcgt ttgcggattg taacccgccg ccaacgcacg ctctatttcg
     7021 cctaacgaga cggaatccac tttcacgccc tgctcacgca ttaagcgcaa aatatgaata
     7081 ttggaacagg ctttctgtgc aaagcgcacc acttcaaact gtttcagcgc tgctatctgc
     7141 cgacgaataa tttgcgcatc gtagacccac accgggcagc caaattcagc gggcaaacgc
     7201 agcagatttt cggcggtgag atcggtatcg gtgctgaaca gtgaatgtgg cataacaaac
     7261 tccagatgag tgctttttta tgattacgcc acatcataaa aagaataaaa aatatcgatt
     7321 tatgtcgaat ctatgcaaaa atgatatgga ttaccggatt gcgagagagc gctaatggcc
     7381 gccgttaact tacgtcatat tgaaattttt catgcggtaa tgaccgccgg aagcctgact
     7441 gaggcagcac acctgctaca cacctcacag ccaaccgtca gccgcgaact ggcgcgcttt
     7501 gagaaggtga tcgggctgaa attgtttgag cgcgtacgtg gacgattaca tcctaccgtg
     7561 caaggactgc gtctgtttga agaagtgcaa cgatcctggt acggactgga tcgcattgtc
     7621 agcgccgcag aaagtctgcg cgagtttcgc cagggagaac tgtctattgc ctgcctgccg
     7681 gtcttttcgc aatctttttt accgcagctc ctgcaaccct ttctggcacg ttatcccgat
     7741 gtcagcttaa atatcgtgcc ccaggaatca ccgctacttg aagagtggct ctcggcccag
     7801 cgtcatgatt taggactcac tgagacactc catacgcctg cgggaacaga acgtaccgaa
     7861 ttactctctt tagatgaagt gtgtgtgtta cctccgggtc atccgctggc ggtaaaaaag
     7921 gtattaacgc cggatgattt tcagggtgag aattacatca gcctttcccg tactgacagc
     7981 tatcgccagt tgctggatca gctatttact gaacatcagg ttaaacgacg catgatcgta
     8041 gaaacccaca gcgccgcgtc agtctgcgca atggtacggg cgggggtagg tgtttcggtg
     8101 gttaacccgc tcaccgcact agattatgcg gcaagcggtt tagtggtgcg gcggttcagt
     8161 attgcggttc cgttcaccgt cagcctgatc cgccccctgc accgcccgtc atcagcgctg
     8221 gttcaggcgt ttagtgggca tttacaagcg gggttaccga aactggtcac ttctcttgac
     8281 gctattttgt cgtcagctac gacagcataa aagcgacagc atcctcggca tggatcgacg
     8341 cggtatcaaa cacaggcaaa acactgcgct cttctggcac cagtaaacca atttctgtgc
     8401 agccaaaaat gacgccctgt gcgccctgtt ctgcaaggcg agcaatcact tgcgcataat
     8461 aagcgcgtga cgcttcggta aatttcccca gacacagttc ttcaaaaata atctgattaa
     8521 ttttcgcccg ttcatccgct tcaggaataa gacaattgat ggaaaattgt tccgtcagcc
     8581 gcccgcgata aaaatcctgt tccatggtgt aacgcgtacc cagcagcgcc acacgagtca
     8641 ttccggcccc ggtaattgca cgtccggtgg catccgcaat gtgtaaaaaa ggcagagtgc
     8701 aacgtgactc aatggcatcc gccactttat gcatcgtatt ggtacataac acaatacctt
     8761 ctgcgcccgc ccgctgtaag ccaagcgccg cctcagccag aatgtccccg gttttatccc
     8821 attccccgcg acgctggcac tcttctattt catgaaaatc gacgctatgt agcagcattt
     8881 gcgcagagtg aagcccacca agccgctgtt taatgccttc atttatcaga cgatagtaag
     8941 gaatggtgga ttcccagctc attcctccca gcaaaccaat tgttttcatt cctctctcct
     9001 gatgtgtgtt accgcaacaa ggctgaataa ggaacgagac aaacgcctca acggccaggt
     9061 gcccaatctc tattaacgaa aaaagggccg gatgtacagc acatccggcc cgtgaaatca
     9121 gacgccgata tttctcaact tctcgcctgc catcagtttg cgttcgatat gttccagcgt
     9181 gacatttttg gtttccggaa tgagccagaa agtaatgccc acaaacgcaa tgttcaacgc
     9241 agtgtagagc cagaacgtac cggcagcgcc aatgctatca agcagtgtca ggaaggccgc
     9301 gccgataatc atattcgaca cccagtttgt cgtcgtcgaa caggtaatac cgaaatcgcg
     9361 gcatttcagc ggctgaattt cagagcacag gatccacacc actggcgcgg cgctcatcgc
     9421 ataaccggca atacacatca tcgtcatgcc aacagagagc caggacaagc cactggaagc
     9481 cgtaccgtta tcaaactgca tcaggcaata gcccagcacc agagtgccta acgccatcac
     9541 gctgaaacca attttcagag ccggtttacg acctgcttta tctaccgtaa agaccgcaat
     9601 gaaggtcgcg aacataaagg tcagtcccac gaccagagtc gcaatcatct gttgttctgt
     9661 ggtcgtaaag cccgccattt tgaagatacg cggcgcgtag tacatgatga tgttcatacc
     9721 ggtaaactgc tgcatcgcct gcaacaacat accgagaaac acagcacgac ggacgttacg
     9781 gttgatctta aacagtgccc aaccgccctg ttttaacttc aggctttcac gaatttcgtt
     9841 gagttcttct cgcgcttttt ccgacgtatc gcgcagcata cgcaacactt cttccgcctc
     9901 aatatgacgc cccttttccg ccagccagcg cgggctattt ggcaggaaga ccaccagaat
     9961 aatcagcaga actgctggta aagcaagaac cccaacattg cgcgccagtt accgctataa
    10021 ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat caactggtac
    10081 atactgatca tcttaccgcg aacgtacttg ccatttcaga aagatacaga ggagcggtgt
    10141 aagacgcgat cccgacagca atgcccagca ccacacgagc ggcgattaac atctctacgc
    10201 tggtcgcaaa agcggaccct atagaaccga gtacaaacag gatggcccca ggcggaacga
    10261 cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac taaccaccca
    10321 ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg ctccggcgat
    10381 tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg aaacaaacat
    10441 attcatacgc cgcgtatcac gcaaagaacg tggcgttaaa gcagattccg tattgatagt
    10501 aaccattttt tcctgccagc agagagtaag acattgtgaa aaaatacgtg aacaattcac
    10561 acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca tgccgttatg
    10621 tgatggatat tccaatttta aaattaagtt gaattattga gattattatt aaccacctaa
    10681 ttttaaagta gataaaattc ataaagttca ttaattgata attgatatgg attatttcat
    10741 aaccatgata tggattatga tgatctagag atataaaaaa ccctgccatg cggcagggtc
    10801 ataaaagtaa gaggaatgaa ttaacgcgcc agccaaccgc catccacagc aatggtataa
    10861 ccatttacat aatctgaagc gctggaggca agaaacacta ccggccccat caggtcactc
    10921 ggcagtcccc aacggccagc tggaatgcga tcgagaattt ccgcgctacg ttgttcatct
    10981 gcccgcagtt gttgggtatt gttggtcgcc atgtaacccg gagctatcgc attaacatta
    11041 atgttgtgtt tagcccattc gtttgccatc aatcgcgtca cacccatcac tccgcttttt
    11101 gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga cgcgatattg
    11161 ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg agacatgaag
    11221 aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct gaactcgaga
    11281 gcgtcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg accaaattca
    11341 gctaccgcgc gatccagcag tccaggaatg ccatcaatct ttcgcagatc ggcggtcagg
    11401 cttaaaaaac gacgccccag cgccgtgacc tgctcgatgg tttcagtcgg ttcaacgatg
    11461 ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc ctggcccagc
    11521 ccagtatcac aaccagtgac gaccgcaact ttaccttgga gagaaaatgc acttaaaatc
    11581 ataacaacac cttattcgtt tatgcccaca actagcgcaa atctttaacg gccacatggt
    11641 ccatatcatt aaagacctgg ttttcaccga ccatgcccca gataaaggta taagctttgg
    11701 ttccgacgcc ggaatggatc gaccagctcg gggagatcac cgcctgctcg ttatgcatca
    11761 caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg tcatcatcca
    11821 tattgaaata gaaataaact tccatccggc gctcgtgggt gtgacacggc atggtgttcc
    11881 acaagttacc tggagccagc tccgtcagcc ccatactcaa ttgacaggtt tccagtacat
    11941 ccgggacaaa atatttgtta atcgtgcgac ggttactggt gaggttatcg cctaacgtga
    12001 ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc gcgggtgcgc
    12061 aattgtaata aaacttcgcc ggagtggcgg tatcaatact ggcaaagaca acttcttttg
    12121 cacctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattga ccatcgacag
    12181 taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca aggaaatagc
    12241 ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt atcggcataa
    12301 tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca tcggcgacaa
    12361 ataccttttc aaccaaaaat tcattacgca gcccttgggt atccagcgtt tttgcgtgcg
    12421 caccgtggat gctctgtctt acgtccacat caacctccga taatcagtga agcgtgaaaa
    12481 tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata tgcccatggg
    12541 ctagggtttt caattaaaaa taaaacaatg ttttattttt aatttaagga ttaaaaaaag
    12601 tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc agaaaatgta
    12661 tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt tatcggtaag
    12721 gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgcccta gcccccgccg
    12781 atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg aaccagagaa
    12841 accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc gccaccattg
    12901 acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc ctgtgcagca
    12961 aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac acgctccagg
    13021 caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac accaacgctg
    13081 gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt ggcttcgctc
    13141 atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc ggtcacagaa
    13201 ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc gtcagtgcgt
    13261 ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat taccgggacg
    13321 atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg cgagctaagt
    13381 gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt ttcggcggtg
    13441 acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag actgtcaacc
    13501 aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg tggtgcgcgg
    13561 ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg tatcgcctga
    13621 gtagccagat gcagtgcttt aagaccggaa ccgcaaacgt cattaatcgt gattgcagaa
    13681 acgctgttag gaagaccacc tttaatagcc gattgccttg ccggattctg ccctgcccct
    13741 gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac gccggtacgt
    13801 tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc cgctaacgca
    13861 ccacgaaagc agccgatagg tgtccgtaac gccccgacaa ccacaacgtc tttcatcaca
    13921 acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtta tcttaattgt
    13981 ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca gagtcataac
    14041 tggcacgtaa ggtttgcaac cactaaccca ccaatagatg ggtagatagg gcgttaatct
    14101 cccatactta acttggttta tggtaaattg ccctccattt tgtttaattt gtagatgata
    14161 cgttcagata atgtctaata tttggtctaa agaagaaact ctgtggagtt tcgcgctcta
    14221 cggcacagcc gttggtgcag gcacgctatt ccttcctatt cagttaggtt cggcaggggc
    14281 tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattagc cacataaagc
    14341 cttatgccag ttcatcctct catcaaaaac atcagcaggt gaagggataa cgggcgcggt
    14401 aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact tcatcgcctt
    14461 ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg aacagctggc
    14521 aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgggtg ttgtattaat
    14581 tctgaatctc atttttctga tgggacgtca tgccactatt cgggtaatgg gatttttggt
    14641 attcccattg attgcctatt tcttatttct ttccattt
//
LOCUS       DAJTNM010000098        14331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTNM010000098 DAJTNM010000000
VERSION     DAJTNM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.098"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..1063
                     /gene="rstB"
                     /locus_tag="OO172_003024"
     CDS             <1..1063
                     /gene="rstB"
                     /locus_tag="OO172_003024"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5513135.1"
                     /translation="PLSKYHLDDISMHRLRGGEIVALDDQYTFLQRIPRSHYVLAVGP
                     VPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPHWQDMLKLEAAAQRFGDGHLNER
                     IHFDEGSSFERLGIAFNQMADNINALIASKKQLIDGIAHELRTPLVRLRYRLEMSDNL
                     SAAESQALNRDISQLEALIEELLTYARLDRPQNELHLSEPDLPLWLSTHLADIQAVTP
                     DKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRYCHSTVETSLLLSGNRATLIVED
                     DGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLAIVHSIALAMGGTVNCDISELGG
                     ARFSFSWPLWHNIPQFTSA"
     gene            1139..2068
                     /gene="tus"
                     /locus_tag="OO172_003025"
     CDS             1139..2068
                     /gene="tus"
                     /locus_tag="OO172_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5513136.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2065..3468)
                     /gene="fumC"
                     /locus_tag="OO172_003026"
     CDS             complement(2065..3468)
                     /gene="fumC"
                     /locus_tag="OO172_003026"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5513137.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3611..5257)
                     /gene="fumB"
                     /locus_tag="OO172_003027"
     CDS             complement(3611..5257)
                     /gene="fumB"
                     /locus_tag="OO172_003027"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5513138.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5456..6631
                     /gene="manA"
                     /locus_tag="OO172_003028"
     CDS             5456..6631
                     /gene="manA"
                     /locus_tag="OO172_003028"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5513139.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLRDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANKSPVTVKGHGRLARVYNKL"
     gene            6732..8240
                     /locus_tag="OO172_003029"
     CDS             6732..8240
                     /locus_tag="OO172_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5513140.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8466..9731)
                     /gene="uidC"
                     /locus_tag="OO172_003030"
     CDS             complement(8466..9731)
                     /gene="uidC"
                     /locus_tag="OO172_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5513141.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSIRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(9770..9937)
                     /locus_tag="OO172_003031"
     CDS             complement(9770..9937)
                     /locus_tag="OO172_003031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR5513142.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(9953..11143)
                     /gene="uidB"
                     /locus_tag="OO172_003032"
     CDS             complement(9953..11143)
                     /gene="uidB"
                     /locus_tag="OO172_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR5513143.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQV"
     gene            complement(11140..12951)
                     /gene="uidA"
                     /locus_tag="OO172_003033"
     CDS             complement(11140..12951)
                     /gene="uidA"
                     /locus_tag="OO172_003033"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5513144.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13339..13929)
                     /gene="uidR"
                     /locus_tag="OO172_003034"
     CDS             complement(13339..13929)
                     /gene="uidR"
                     /locus_tag="OO172_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5513145.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14158..>14331)
                     /locus_tag="OO172_003035"
     CDS             complement(14158..>14331)
                     /locus_tag="OO172_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5513146.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3604 a         3744 c         3407 g         3576 t
ORIGIN      
        1 gccactgagt aaataccatc ttgatgatat ttccatgcac cgactgcgtg gcggcgaaat
       61 tgtcgccctg gacgatcagt acacgttttt gcagcgtatc ccgcgcagcc actacgtgct
      121 ggcagttggt cctgttcctt atctttatta cctccatcaa atgcgattgc tggatatcgc
      181 cctgatcgct tttattgcta tttccctcgc ctttccggtg tttatctgga tgcgtccgca
      241 ctggcaggat atgttaaaac tggaagcagc ggcgcaacga tttggcgatg ggcatctcaa
      301 tgaacgtatc cactttgatg agggttcgag ctttgaacga cttggtatcg catttaacca
      361 gatggcggac aatatcaacg ccttaattgc cagcaaaaaa cagcttattg acggtatcgc
      421 tcacgaactg cgaacaccgt tagtgcgcct gcgttatcga ctggagatga gcgataacct
      481 gagcgccgcc gaatcccagg cgttgaatcg tgatatcagt caacttgaag ctttaattga
      541 agagctgctg acttatgccc gactcgatcg cccacaaaac gagcttcatc ttagcgaacc
      601 agacctgccg ttgtggctgt caacgcatct ggcagatatt caggcagtaa cgcccgataa
      661 aacggtacgg ataaaaacgc tcatgcaagg ccattatgcg gcgttggata tgcgcttaat
      721 ggagcgcgtg ctggataatt tgctcaataa cgccctgcgc tactgccatt caacggttga
      781 aaccagcctg ctactgtcgg ggaatagagc gacattaatt gttgaggatg atggcccagg
      841 gattgcccca gaaaaccgcg aacatatctt tgaacctttt gttcgcctcg atcccagccg
      901 ggatcgctca accggcggct gcgggctggg gctggcaatt gtccactcta tagcactggc
      961 aatgggcggt acggttaatt gtgacatcag cgaactgggt ggtgctcgct tctcgtttag
     1021 ctggccgtta tggcataaca tcccgcaatt tacctctgcc tgacactacg cgcacgatgg
     1081 tcaagtcaca acgactgtgc tataaaataa gtatgttgta actaaagtgg ttaatattat
     1141 ggcgcgttac gatctcgtag accgactcaa cactaccttt cgccagatgg aacaagagct
     1201 ggctacattt gccgctcatc ttgagcaaca caagctattg gttgcccgcg tgttctcttt
     1261 gccggaggta aaaaaagagg atgagcataa tccgcttaat cgtattgagg taaaacaaca
     1321 tctcggcaac gacgcgcagt cgctggcgtt gcgtcatttc cgccatttat ttattcaaca
     1381 acagtccgaa aatcgcagca gcaaggccgc tgtccgtctg cctggcgtgt tgtgttacca
     1441 ggtcgataac ctttcgcaag cagcgttggt cagtcatatt cagcacatca ataaactcaa
     1501 gaccacgttc gagcatatcg tcacggttga atcagaactc cccaccgcgg cacgttttga
     1561 atgggtgcat cgtcatttgc cggggctgat cacccttaat gcttaccgca cgctcaccgt
     1621 tctgcacgac cccgccactt tacgctttgg ttgggctaat aaacatatca ttaagaattt
     1681 gcatcgtgat gaagtcctgg cacagctgga aaaaagcctg aaatcaccac gtagtgtcgc
     1741 accgtggacg cgcgaggagt ggcaaagaaa actggagcga gagtatcagg atatcgctgc
     1801 cctgccacag aacgcgaagt taaaaatcaa acgtccagtg aaggtgcagc cgattgcccg
     1861 cgtctggtac aaaggagatc aaaaacaagt ccaacacgcc tgccctacac cactgattgc
     1921 actgattaat cgggataatg gcgcgggcgt gccggacgtt ggtgagttgt taaattacga
     1981 tgccgacaat gtgcagcacc gttataaacc tcaagcgcag ccgcttcgtt tgatcattcc
     2041 acggctgcac ctgtatgttg cagattaacg cccggctttt atactgccga ccatctgttc
     2101 tggccgtacc cagctgtcaa actcggcttc gctaagatac cccagcgcaa gggccgcagc
     2161 ttttaaggtc agcccttctt tatgcgcttt tttggcgatc tccgcggctt tgtcataacc
     2221 aatgtgggtg ttaagcgcag tcaccagcat cagcgattca ttgagtaatt gattgattcg
     2281 ctcacgattc ggttcaatac ccactgcgca gtgtttgtta aaactttcca tgccatctgc
     2341 cagcaagcgc accgattgca ggaaattatg gatcaccatc ggacggaaga cgttcagttc
     2401 aaagttaccg gaagcgccac ccatgttgat cgccacgtcg ttccccatta cctgacagca
     2461 gagcatggtt aatgcttcac actgggttgg gttcactttc cccggcatga ttgagctgcc
     2521 cggctcattt tccgggattg agatttcacc aattccgcag cgcgggccag aggccagcca
     2581 gcggacatca ttggcgattt tcatcagtga cgcagccaac cctttcaacg cgccgtgcgc
     2641 ctgaaccagg gcatcacagg tcgcaagcgc ttcaaatttg ttcggcgcgg taacaaacgg
     2701 tgcacaggta atgactgcca gttcatctgc tacgcgccgc gcatactccg gatgggtatt
     2761 tagtccagta cccaccgctg taccgccaag agccagttcc gctacgtgag gcaggctgta
     2821 ttcgatatgt ttgagattat gctcgagcat cgctacccag ccggaaatct cctgccctag
     2881 tgttagcggc gtggcgtcct gcaagtgggt tcgaccgatt ttgacgatat cggcaaatgc
     2941 acgcgatttt tcactcagcg tctgtgtcag ggttttaagc tgcggaatga gttgcttgcg
     3001 cagtgccagc agcgccgcaa cgtgcatcgc cgtcggaaag acatcgttgg aactttggct
     3061 tttgttcacg tcgtcgttag ggtgaacttt acgttccatc ccgcgcacac cgccgagtaa
     3121 ttcactggcc cggttagcca gcacttcgtt catgttcatg ttactttgcg tgccggagcc
     3181 ggtctgccag atagccagcg ggaattcgtc gtcatgctgt cctgccagta cttcatccgc
     3241 cgcctgccga atggcgctcg ctttctcttc agacaacaag tctaaatctt cattaacttt
     3301 tgccgctgcg cgcttggtta gcgccagcgc atgaatcagt gaggtgggca ttttctccgt
     3361 cgaaatgcgg aaatgctcca gcgagcgttg agtttgtgcg ccccacagct tatctgccgg
     3421 gacatcaatc gcccccatcg aatctttttc gctgcgtact gtattcatgg cctgctcctc
     3481 acctgattaa ttttttcttt ctgttttgct ttcgttaagc aacttttagc tcactcatta
     3541 tttactattt gataacaaat gtttggtctt tcgtgccatg taaaaaaacc gccccgaagg
     3601 gcggctctgt ttatttcaca cagcgagtgc actgtgtgag ttgtatctgc tggaagaagt
     3661 catttccttt atcatccaca aggataaacg ccgggaaatc ttccacttca attttccaga
     3721 tggcttccat tcccagttcc ggatattcaa cacattccag gctcttaata cttccctgcg
     3781 ccaatacagc ggccggacca ccgatactgc caaggtagaa gccgccgtgt tttttacagg
     3841 catccgtcac ctgctggctg cggttgcctt tcgccagcat gatcatactt ccgccctgcg
     3901 cttgcagttg atcgacataa gaatccatcc gtccggcggt cgttgggcca agagaaccgg
     3961 aggcataacc ttccggcgtt ttggccggac ccgcgtagta aatcggatga tctttaatgt
     4021 attgcggcag cccttcaccg ttatccatcc gctctttcag tttggcgtga gcaatatcac
     4081 gaccaacgat aatcgtgccg ttaagcgata agcgtgtaga aacgggatac tgcgacaact
     4141 gtgcgaggat ctctttcatc ggacggttaa ggtcaacgcg caccgcttcg ccttctcccg
     4201 ctttacgcag ctcttcaggg atatatttac ctggattatg ttccagtttt tcgatccaga
     4261 tcccctgacg gttgatcttc gctttgatat tacggtcagc agagcaggaa acacccatac
     4321 caaccggaca ggatgcgccg tgacgtggca ggcgaatcac gcggatgtcg tgagcgaagt
     4381 atttaccacc aaactgcgca cccagaccga gattttgcgc ttcgatcagt aattcttttt
     4441 ccagttccac atcgcggaac gcctgaccgt gctcattccc ttccgttggc agttcatcat
     4501 agtatttcgc ggaagccagt ttcaccgttt taaggttcgt ttctgcagaa gttccaccaa
     4561 taacgaacgc aatatgatac ggaggacagg ccgcggtacc cagcgtgcgc atcttctcaa
     4621 ccaggtaatt tttcagtttc cccggcgtca gtaacgcttt ggtttcctga tagagatacg
     4681 tcttgtttgc cgaaccacca ccttttgcga tacagaggaa tttgtattcg tcgccatcaa
     4741 cggcataaag atcgatctgc gctggcagat tggtgccggt attcacttct ttatacatat
     4801 ccagcggcgc gttttgcgag tagcgcagat tatcttcgat ataagtgtta tagacaccgc
     4861 gcgccagcgc cgcttcatca ccaccaccgg tccatacacg ctgccctttt ttaccaacaa
     4921 taatcgcggt gccggtatcc tgacaggttg gcagaacgcc tttcgccgcg atgtcggagt
     4981 tacgcaggaa ttgcagcgcc acatatttat cattttcgct ggcctccggg tcacgcagaa
     5041 tgtcggccac ctgttgttgg tgagccggac gcagcataaa cgacgcatca tgaaacgcct
     5101 gacgcgccaa cagagttaac gcttcgggtg cgactttcaa aatctcctgc ccttcaaatt
     5161 cagatacgct aacgtgttca ctggttagca ggtaatactc agtatcatct tttttgagtg
     5221 gaaaaggaga ctgataatga aagggtttgt ttgacattgt tctctcactt actgcctggt
     5281 ttggttatgc tctgggcggg tgttccgttg ccctgttaaa agcgagtaac aatatcctac
     5341 acagtttttt aacaaaaact gagactagta cgactttttg cggctccagg ttacttcccg
     5401 taggattctt gctttaatag cgggattaat ttccacatta aaacagggat tgatcatgca
     5461 aaaactcatt aactcagtgc aaaactatgc ctggggtagc aaaacggcgt tgactgaact
     5521 ttatggtatg gaaaatccgt ccagccagcc gatggccgag ctgtggatgg gcgcacatcc
     5581 gaaaagcagt tcacgagtgc agaatgccgc cggagatatc gtttcactgc gtgatgtgat
     5641 tgagagtgat aaatcgactc tgctcggaga ggccgttgcc aaacgctttg gcgaactgcc
     5701 tttcctgttc aaagtattat gcgcagcaca gccactctcc attcaggttc atccaaacaa
     5761 acacaattct gaaatcggtt ttgccaaaga aaatgccgca ggtatcccga tggatgccgc
     5821 cgagcgtaac tataaagatc ctaaccacaa gccggagctg gtttttgcgc tgacgccttt
     5881 ccttgcgatg aacgcgtttc gtgaattttc cgagattgtc tccctactcc agccggtcgc
     5941 cggtgcacat ccggcgattg ctcacttttt acaacagcca catgccgaac gtttaagcga
     6001 actgttcgcc aacctgttga atatgcaggg tgaagaaaaa tcccgcgcgc tggcgatttt
     6061 aaaatcggcg ctcgacagtc agcagggtga accgtggcaa acgattcgtt taatttctga
     6121 attttacccg gaagacagcg gcctgttctc cccgctgtta ctgaatgtgg tgaaattgaa
     6181 tcctggcgaa gcgatgttcc tgttcgctga aacaccgcac gcttacctgc aaggcgtggc
     6241 gctggaagtg atggcgaact ccgataacgt gctgcgtgca ggtctgacgc ctaaatacat
     6301 tgatattccg gaactggttg ccaatgtgaa attcgaagcc aaaccggcta accagttgtt
     6361 gacccagccg gtgaaacaag gtgcagaact gggcttcccg attcctgtgg atgattttgc
     6421 cttctcgctg catgacctta gggataaaga aaccaccatc agccagcaga gtgccgccat
     6481 tttgttctgc gtcgaaggcg atgcaacgtt gtggaaaggt tctcagcagt tacagcttaa
     6541 accgggtgaa tcagcgttta ttgccgccaa caaatcaccg gtgactgtca aaggccacgg
     6601 ccgtttagcg cgtgtttaca acaagctgta agagcttact gaaaaaatta acatctcttg
     6661 ctaagcttat taaaggctta taacaccttc aggcggccag tccgcctgat ttcattttat
     6721 ggataatcat tatgaataaa tcgctggtag cggtaggcgt cattgttgcg ctaggcgtag
     6781 tctggacagg cggcgcatgg tatacaggca agaagattga aacccatctc gaagacatgg
     6841 tcgcgcaggc gaacgcgcaa ctcaaactga cagctcctga atccaacctg gaagtgagtt
     6901 atcaaaacta tcatcgcggc gtattcagca gccagttgca actgttggtg aaacccattg
     6961 ctgggaaaga aaatccgtgg attaaaagcg gtcagagcgt catcttcaac gaatcggttg
     7021 atcatggtcc cttcccgctt gcccagctta aaaaactgaa cctgatcccg tcgatggcat
     7081 caattcaaac cacgctggtt aataacgaag taagcaaacc actgtttgat atggcaaaag
     7141 gtgaaacgcc ttttgagatt aactcgcgca ttggttacag cggtgattcc agttccgata
     7201 tttcgctcaa tccactgaat tacgagcaaa aggatgaaaa agtcgccttt agcggcggcg
     7261 agttccagtt aaatgctgac agagacggca aagccatctc cctttccggg gaggcgcaaa
     7321 gtggtcggat agacgcagtt aacgaataca accagaaagt gcagttgacc tttaataatc
     7381 tgaaaaccga cggttccagc acgctgggaa gttttggtga gcgcgtagga aaccaaaaac
     7441 tgtcactgga aaaaatgacc atttcagtgg aaggcaaaga actggcactg ctggaaggca
     7501 tggagatcag cggtaaatcg gatctggtca atgacggtaa aacgatcaat agccaactgg
     7561 attactcgct aaacagcctg aaggtacaga atcaggatct gggcagcggc aagctgactt
     7621 taaaagtcgg ccagattgat ggtgaagcct ggcatcagtt tagccagcaa tataacgcgc
     7681 aaactcaggc gctgctggca cagccagaaa ttgccaacaa tcccgaactt tatcaggaga
     7741 aagtgacgga agccttcttt agcgccctgc cgctgatgtt gaaaggcgat ccggtgatta
     7801 ctatcgcgcc gctaagctgg aaaaacagtc agggtgaaag tgcgctgaat ctgtcgctgt
     7861 tcctgaaaga tccggcaacg actaaagaag cgccgcaaac gctggcgcag gaagtagatc
     7921 gttcggttaa atctctggat gcgaaactga ccattccggt ggatatggca actgagttga
     7981 tgactcaggt agcgaagctg gaaggttatc aggaagatca agcgaaaaaa ctggcgaaac
     8041 agcaagttga aggtgcatca gcaatggggc agatgttccg tctgaccacc ttgcaggaca
     8101 ataccatcac caccagcctg caatatgcta acggtcagat aacgttaaat gggcagaaaa
     8161 tgtcgttaga agattttgtt ggcatgtttg cgatgccagc tcttaacgtt ccggctgtac
     8221 cggcaattcc gcagcagtaa ttcacggcca aaacgccgga gaattttcac tccggcgttt
     8281 tcatctgaaa acaaaccaga gattgcctga tgcgctacgc ttatcaggcc taccttttcc
     8341 tgcaacactt tgaatttatg agtttttgta ggctggataa ggcgtttacg ccgcatccgg
     8401 cataaaaaac gcgcactttg tcaacaatct gaaacgccgg aggtttttct ctccggcgtt
     8461 atttcttact tcagcataaa gtcatactga atactcattt tcacatccca gtaattcagg
     8521 cgatcgccca gcgtactgcc atcgcgcttt tcccgccctt tatcggcagc aagaaagaaa
     8581 ttcatccctt gtaacggccc ttcgctgacc cgatagttca taccacctaa gatacccgcg
     8641 ccttcaatcc gcacatctcg tttactggat tcatagccgc gagtcacaat cggtgcaaat
     8701 gtcagcgtaa attgcggagt tacacggtag ttgacaccta attgccagga ctgcattcct
     8761 tcacggttac gatcaagact taatgaaaag ggatagccaa tgtcggtatc caccacggca
     8821 ccattcattg catggcgcaa ataaccacca ctgccaaata cgcttcccca cggcgcattc
     8881 caggtcaatt gtccactaac caacgccgtt tcattgggct ggctggtatt acgacttaaa
     8941 ccttcgagct gcgcgtaaaa gccgagcaac tcccctttca acgtataatt cgggtcgagc
     9001 ggtatgcttt tctcgagaat actttgtatc tgattggaaa catctttctg taccgatgcc
     9061 cccagagaat agcgactggt atcatcatgc caactggcgg ctaaaaaaga acgtgcgcga
     9121 tgtttatcgt attttcccgt ctggctgtta tacacccctt catcacgaat accggttgtt
     9181 gaatcagtcc ataaaaatac tcgtgaacgc cacattcccc acagatcaat atttggtgtt
     9241 aaagcccaat aacctaatgc tccttcagaa actgtcggta atgtacgttg cgaactgcca
     9301 gccattaacg gaatacgata aggtaagcta ccataactat aatccgtccc gaagcgacct
     9361 aatttaagca ggctattttc actgggtttt atttttactg cccccagggc aaagccaaaa
     9421 cttttatcac catcaagata ccaccggata ctcatatcag cacgagcacc taatttataa
     9481 acataatacg ctccgccttc aacgccaata atattggaat aataaccact attgaaatcg
     9541 agcaatcctc cctgtaccca tgcgtaaata tctcttcccg cgccagcact gggcttatca
     9601 gccctgcgca attcgttttt cagtctgatg cgtagtcccg cttcatcgtc agccagttgc
     9661 gccacgtaag cagaggtaag gccagaggca gccgtcaggc aaataacggc catggccact
     9721 atttttctca tagttgcacc tttgatgttc tgattatttt tattgaatat taattagtga
     9781 tatcgctgat taattgctgc tgcacttttt tacgattatc aatttcaacc acgatttctt
     9841 tgaatttttt atccgtgagc ggataaaacc agataataac gaatgccagt agcataaatc
     9901 cgcaaggtac taaggcaatt gatgtgcgga tgcccataat aacttccggc gtttacacct
     9961 gattggcgat atatccgctt aatcccaaaa taaaggcagg aattgaacct ccgattgcct
    10021 gaccgcattt acgggtaaat gagaatagtg aataggtgag cccttcaatt cgcacgccgg
    10081 tcaggtattc accgtattct acggtatcag cttccagtgc ccacatcaca gtcatagtaa
    10141 cgccctgacc aattgaagcg atggccaacg caaccaacgc caccggcagt gaccagacgg
    10201 aaacccagaa gaatagcaga taaccgcagg ttcccagcaa agcgccaatc aggaaggtat
    10261 tctttttacc gatcctcgcg accatccccg gcacaagcgg tgccgatgcc acagtaccaa
    10321 ccaggttttg caccagtacc agcacagtga acagcccggt atcatttaac acatagcgca
    10381 cgtagaacaa cgacgaggcg ctgaccgcga aggtcgaaat cagcacacac agcgcaccga
    10441 tgcacaacat aaacagcggg cgattccgtt tcagggtttg caaactgatc ttcaatgacg
    10501 gctgcgccac gatacgtacc acattttcac gcgtcgattt gaagcagatg aagtaaagca
    10561 ccattccggc aatcgccagc acaatcgtcc agaaatggta taccgacacc atctcttccg
    10621 ggctggagtt cttaatgctc ggtcctatca gaaatgccag gcatacaaag gtcaatgaag
    10681 cggcaatccc acgagccgcg cccagacggg cgcgagattg tggttgttgg gtcatcgcgg
    10741 tagcaagtga accataagga atattcacca ggctgtagca aagcccgagg cccatgtagg
    10801 tcaaatatgc atacaccact ttgctaccat ggctccagtc ggtcggtacc cagaatacca
    10861 gcacgctgaa gatcattaac ggcgcagtac cgaagagtaa aaacgggcgg aattttcccc
    10921 agcgggtatt cacactgtcc accactcgtc cggcaaagac gtcggcgaag gcatcaaata
    10981 cccgcaccag taacagcatg gtgcctgccg cagcggcacc gacgccagcg acgtcggtgt
    11041 agtaactcaa taggaagagc gcccccattg cgaaggcgaa gttattggcg acgtcaccga
    11101 ggctgtagcc gacgatggtg cgccaggaga gttgttgatt cattgtttgc ctccctgctg
    11161 cggtttttca ccgaagttca tgccagtcca gcgtttttgc agcagaaaag ccgccgactt
    11221 cggtttgcgg tcgcgagtga agatcccttt cttgttaccg ccaacgcgca atatgccttg
    11281 cgaggtcgca aaatcggcga aattccatac ctgttcaccg acgacggcgc tgacgcgatc
    11341 aaagacgcgg tgatacatat ccagccatgc acactgatac tcttcactcc acatgtcggt
    11401 gtacattgag tgcaggccgg ctaacgtatc cacgccgtat tcggtgatga taatcggctg
    11461 atgcagtttc tcctgccagg ccagcagttc tttttccagt accttctctg ccgtttccaa
    11521 atcgccgctt tggacatacc aaccgtaata acggttcagg cacagcacat caaagagatc
    11581 gctgatagta tcggtgtgag cgtcgcagaa cattacattg acgcaggtga tcggacgcgt
    11641 cgggtcgagt ttacgcgttg cttccgccag tggcgcgaaa tattcccgtg caccttgcgg
    11701 acgggtatcc ggttcgttgg caatactcca catcaccacg cttgggtggt ttttgtcacg
    11761 cgctatcagc tctttaatcg cctgtaagtg cgcctgctga gtttccccgt tgacagcctc
    11821 ttcgctgtac agttctttcg gcttgttgcc cgcttcgaaa ccaatgccta aagagaggtt
    11881 aaagccgaca gctgcagttt catcaatcac cacgatgcca tgttcatctg cccagtcgag
    11941 catctcttca gcgtaagggt aatgcgaggt acggtaggag ttggccccaa tccagtccat
    12001 taatgcgtga tcgtgcacca tcagcacgtt atcgaatcct ttgcctcgca aatccgcatc
    12061 ttcatgacga ccaaagccag taaagtagaa cggtttgtgg ttaatcagga actgttcgcc
    12121 cttcactgcc actgaccgga tgccgacgcg cagcaggtag atatcacact ctgtctggct
    12181 tttggctgtg acgcacagtt catagagata accttcaccc ggttgccaga ggtgcggatt
    12241 caccacttgc aaagtcccgc tggtgccttg tccagttgca accacctgtt gatccgcatc
    12301 acgcagttca acgctgatat caccattggc caccacctgc cagtcaacag acgcgtggtt
    12361 acagtcttgc gcgacatgcg tcaccacggt gatatcgtcc acccaggtgt tcggcgtggt
    12421 gtagagcatt acgctgcgat ggatcccggc atagttaaag aaaccatgga agtaagactg
    12481 ctttttcttg ccgttttcgt cggtaatcac cattcccggc gggatagtct gccagttcag
    12541 ttcgttgttc acacaaacgg tgatacgtac acttttcccg gcaataacat acggcgtgac
    12601 atcggcttca aatggcgtat agccgccctg atgctccatc acttcctgat tattgaccca
    12661 cactttgccg taatgagtga ccgcatcgaa acgcagcacg atacgctggc ctgcccaacc
    12721 tttcggtata aagacttcgc gctgatacca gacgttgccc acataattac gaatatctgc
    12781 atcggcgaac tgatcgttaa aactgcctgg cactgcaatt gcccggcttt cttgtaacgc
    12841 gctttcccac caacgctgat caattccaca gttttcgcga tccagactga atgcccacag
    12901 gccgtcgagt tttttgattt cacgggttgg ggtttctaca ggacgtaaca taagggactc
    12961 ctcattaaga taaaaatact ggtcaacctt taatctgatt agttaaccaa ttctgtgttg
    13021 cacacagtta tctgtgagaa acccatggaa gagaaatgga aataaaaaga actgacaccg
    13081 cgatcaaaaa accaaaaact gcaataccca cagcgtgata tggatcgcat taatctttaa
    13141 aacagcgttg acagaagcca taaaacaagc aatcgagcgt tcgttcgaga gtattgggaa
    13201 cgataaactc tcatgacata tggctacggt gaatattttg ggggggaatt ctccgaacac
    13261 ggggaggata atttttgcac agagaaatta cgatatgaaa cgcgggcaca gttatcacca
    13321 gcccgcgaaa tgagaggatc aggatgcggt taatataccg ccaatcatcg cgcgtaatcc
    13381 ctgagcaaac gacgcttcac gcgccaggtc ttccgcttcg atatcggcca gtacaccgta
    13441 ggtcagatcc agcagtaaac gtgaagtcat tgccgtgttg atgtctgggc ttatctcgcc
    13501 tttttgctgg gcatcacgca tccgctgggc aacaaattca gtgatcgtca tatgcttatt
    13561 tttaagcatg gcggcgacct gtgggttacg catcccttcc gccataattt cgaccaccag
    13621 cgcccgttgc ccaaaggctt catgggtgag agagacaata gactcgacca tatagtcaac
    13681 aaaatgaatc ccttcaatcg gttcccggaa acgagccagc gccctctcct ggtcctgtaa
    13741 gataatcgcc tgaatcaagg cttctttgga gatgaaatgg tgatagagcg tcccaggact
    13801 aatggcgcaa gatttacaga tggctttcat cgaggcactg tgaaatccat tttctgaaaa
    13861 aatctctctg gcagcattga ggatccgggt ccgtgtcggt tgtgcttcag tctgcatgtt
    13921 atccatcatt aaaccattat agttagcgtg aataactagt ttaccgcgtt gtcaggtgag
    13981 cgcctatcat tgctgttgaa tagtgcgaag gtacactcta ttcgtatact tttcacccag
    14041 caattggtta accaattgcc gtgatttggc tgccagttgt ttagcagaga cgttgatgaa
    14101 ttgcacacgc tatcccggta aacaacacat acgaccgttt accggttcgt tagtgtatta
    14161 attgagctcc tgtaccccac caccggagac ggtgagaatt tgtccgctta cccagctggc
    14221 tgcaggcgag caaaggaaca gcgccgcgtt agcaatatct tgcggttggc ccagacgtct
    14281 gatcggcgtg tgctgtaaca ttttttgttc aatttctggg taatgactcc a
//
LOCUS       DAJTNM010000099        14020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTNM010000099 DAJTNM010000000
VERSION     DAJTNM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.099"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..1018)
                     /locus_tag="OO172_003036"
     CDS             complement(<1..1018)
                     /locus_tag="OO172_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5513147.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEV"
     gene            complement(1217..1301)
                     /locus_tag="OO172_003037"
     tRNA            complement(1217..1301)
                     /locus_tag="OO172_003037"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1265..1267),aa:Leu,seq:caa)
     gene            1497..2516
                     /gene="ahr"
                     /locus_tag="OO172_003038"
     CDS             1497..2516
                     /gene="ahr"
                     /locus_tag="OO172_003038"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5513148.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2645..4147
                     /locus_tag="OO172_003039"
     CDS             2645..4147
                     /locus_tag="OO172_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5513149.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4266..5348)
                     /gene="lptG"
                     /locus_tag="OO172_003040"
     CDS             complement(4266..5348)
                     /gene="lptG"
                     /locus_tag="OO172_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5513150.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5348..6448)
                     /gene="lptF"
                     /locus_tag="OO172_003041"
     CDS             complement(5348..6448)
                     /gene="lptF"
                     /locus_tag="OO172_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5513151.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPIRRLRASFSRKGAV"
     gene            6715..8226
                     /gene="pepA"
                     /locus_tag="OO172_003042"
     CDS             6715..8226
                     /gene="pepA"
                     /locus_tag="OO172_003042"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5513152.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8360..8803
                     /gene="holC"
                     /locus_tag="OO172_003043"
     CDS             8360..8803
                     /gene="holC"
                     /locus_tag="OO172_003043"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5513153.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8803..11658
                     /gene="valS"
                     /locus_tag="OO172_003044"
     CDS             8803..11658
                     /gene="valS"
                     /locus_tag="OO172_003044"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5513154.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11712..12908)
                     /locus_tag="OO172_003045"
     CDS             complement(11712..12908)
                     /locus_tag="OO172_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5513155.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSAGMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNSVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILTFLDLLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13101..13604
                     /locus_tag="OO172_003046"
     CDS             13101..13604
                     /locus_tag="OO172_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5513156.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13650..>14020)
                     /gene="rraB"
                     /locus_tag="OO172_003047"
     CDS             complement(13650..>14020)
                     /gene="rraB"
                     /locus_tag="OO172_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5513157.1"
                     /translation="IIEELLEDGSDPDALYTIEHHLSADDLETLEKAAVEAFKLGYEV
                     TDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAEKFDVEYDGWGTYFEDP
                     NGEDGDDEDFVDEDDDGVRH"
BASE COUNT         3605 a         3583 c         3631 g         3201 t
ORIGIN      
        1 aaacctcgac cttcgtgtca taccccatta cgcgcaaagc cttgttcacc gtattctcac
       61 tcatcggctt acgtggatcg tgatcgccca caaaaatcag ctctcgattt ccgctcatgc
      121 ttttgatctt ttccaaaata cttagggcct gccgcgacaa aggtacaagg tgtggtgtcc
      181 gcatcttcga gccacgctga gaatgcttga ctccttccag ttgctcacgc tcacctggga
      241 ttgtccacat agccgtttca aaatctactt ctgaccagcg ggcaaaacgc agctcgctcg
      301 aacggatgaa aaccaataaa gtgagttcaa ccgccagtcg agttaacggt ctgccagaat
      361 agtgatctat gcgatgaagt aattcaggta tacggttcag ttcaagagct gcacggtgct
      421 gtcttttcgc cgtagcaacc gcacctgcaa tctcttgcgc ggggttgtag tcgattaagc
      481 cgctctgaac ggcaaagcgc ataatcgcgg tagtacgctg ctgtaaacgg gcggcaactt
      541 cgagccgccc ggatgactcg actgctttaa tgggtacaag aagatcccgc gtcttcagtt
      601 ccgcaatgtt ccgcttaccg atagcagtaa agagattatc ctccagactt ttcaaaacac
      661 gagcgctatg cgatgccgac cacttctgat tgctggcgtg ccagtctctg gcgactactt
      721 caaacgttat cgcctcttgt tcctgttcta ccttaacagc tttcttgttt tcactgggat
      781 cgacaccatt agctaaaagc ttacgagcct catcccggcg tgccctggca tccgccaacg
      841 acacttcggg gtatttcccc aatgcaagca tcttctcttt gccgccaaag cgataacgta
      901 gccgccagta tttggagccg ttagggtgaa ccagcaaaac catgccttca ccgtcggtca
      961 gcttataggc ttttgcttca ggcttagccg aacgaacctt cacatcactc agagccatga
     1021 tgagtatcct ttcaagggtt ctgtgtgggt acaaacatta tcgaaccggg aaatacccgc
     1081 agttgtaccc gcatcagtaa gttgatgtag attgaatcag gttgacttag gttgagtgaa
     1141 aaagcgagga aagccttgcg gatactggat tttaggcaca aaaaaagacg tccgttgacg
     1201 tctattgatg ttccgatggt gccgaaggcc ggactcgaac cggcacgtat ttctacggtt
     1261 gattttgaat caactgcgtc taccgatttc gccacttcgg cactgaagag gtatgcggaa
     1321 aacgttgtgg attatacctg tcgcacgcca ccatgcaagc gccagcttgc acccacctcg
     1381 ctaagtgctg aaattttcag cattacactc agcgatatgt aacctttgtc acactccagg
     1441 caccccgccc tgccatgctc tacacttccc aaacaacacc agagaaggac ccaaaaatgt
     1501 cgatgataaa aagctatgcc gcaaaagaag cgggcggcga actggaagtt tatgagtacg
     1561 atcccggtga gctgaagcca caagatgttg aagtgcaggt ggattactgc gggatttgtc
     1621 attccgatct gtcgatgatc gataacgaat ggggattttc acaatatccg ctggttgccg
     1681 ggcatgaggt gattgggcgc gtggtggcac tcgggagcgc cgcgcaggat aaaggtttgc
     1741 aggtcggtca gcgtgtcggg attggctgga cggcgcgtag ctgtggtcac tgcgacgcct
     1801 gtattagcgg taatcagatc aactgcgagc aaggtgcggt gccgacaatt atgaatcgcg
     1861 gtggctttgc cgagaagttg cgtgcggact ggcaatgggt gattccactg ccagaaaata
     1921 ttgatatcga gtccgccggg ccgctgttgt gcggcggtat cacggtcttt aaaccactgt
     1981 tgatgcacca tatcactgct accagccgcg ttggggtaat tggtattggc gggctggggc
     2041 atatcgctat aaaacttctg catgcaatgg gatgcgaggt gacggccttt agttctaatc
     2101 cggcgaaaga gcaggaagta ctggcgatgg gtgccgataa agtggtgaat agccgcgatc
     2161 cgcaggcact gaaagccctg tcggggcagt ttgatctcat tatcaatact gtgaacgtca
     2221 gcctcgactg gcagccttat tttgaggcgc tgacctatgg cggtaatttc catacggtcg
     2281 gtgcggttct cacgccgctg tctgttccgg cctttacgtt aattgcgggc gaccgcagca
     2341 tctctggttc tgctaccggc acgccttatg agctgcgaaa gctgatgcgc tttgccgccc
     2401 gcagcaaggt tgcgccgaca accgaactgt tcccgatgtc gaaaattaac gacgccattc
     2461 agcatgtacg cgatggtaaa gcgcgctacc gagtagtcct gaaagccgac ttctgatccc
     2521 tttgccgggt gctgtcgccc ggcacttaaa tctctttgga aggcgtctcg caatctgctg
     2581 aatttagcgg tgaagagtgc acagccgttg cctatactcc aggcaggaaa ctggaggaaa
     2641 tctcatgagc gaacccctgt taattgcccg cacgccggac acagaactgt ttttactgcc
     2701 gggaatggct aaccgtcacg ggctgattac tggcgcaacg gggacgggta aaaccgttac
     2761 gctgcaaaaa ctggcagagt cattgtcgga aatcggcgtg ccggtgttta tggctgatgt
     2821 gaaaggcgat ctgaccggta tcgcgcaggc aggaacggcg tcggaaaaac tgctcacaag
     2881 gcttaaaaat atcggcgtca atgactggca accgcatgcc aatccggtgg tggtgtggga
     2941 tatctttggc gagaaaggcc acacggtgcg ggcgacggtt tcggatctgg ggccgctgtt
     3001 gctggcgcga ctgttgaatc tcaacgatgt gcaatctggc gtgctgaata ttatcttccg
     3061 cattgcggac gatcagggat tgttgctgct cgactttaaa gatttgcggg cgattaccca
     3121 gtacatcggc gataacgcca aatccttcca gaatcagtac ggaaatatca gtagcgcatc
     3181 ggttggtgcc atccagcgcg gattactgtc gctggaacag caaggcgcag cacacttctt
     3241 tggtgagccg atgctggata tcaaagactg gatgcgcacc gatgccaacg gtaaaggcgt
     3301 tatcaatatc ctcagcgccg agaaacttta tcagatgccg aaactgtacg ccgccagcct
     3361 gctgtggatg ctttcagagt tgtatgaaca attgccggaa gcgggcgatc tggagaaacc
     3421 aaaactggtg tttttcttcg acgaggcaca tctgctgttt aacgatgcac cgcaggtact
     3481 gctggataag attgagcagg tgataaggct tattcgctca aaaggcgtgg gcgtctggtt
     3541 cgtttcgcaa aacccgtctg atattccgga taatgtgctc ggccagctcg gtaatcgtgt
     3601 tcagcacgct ttgcgagctt ttacgccaaa agatcagaaa gcagtgaaga cagcggcgca
     3661 aaccatgcgg gccaatccgg catttgatac cgaaaaggca atccaggaac tggggaccgg
     3721 cgaggcgtta atctcgtttc tcgatgcaaa aggaagtcct tctgtggtgg aacgggcgat
     3781 ggtgatcgca ccttgttcgc gaatggggcc ggtgacggaa gatgagcgta atggcctgat
     3841 taatcactcc ccggtgtatg gcaaatatga agatgaggtg gaccgcgagt ccgcctatga
     3901 gatgctgcaa aaaggctttc aggccagtac cgagcagcaa aataatcccc ccgtgaaagg
     3961 gaaagaggtg gcggtggatg acggtattct tggtggattg aaggatattt tgtttggcac
     4021 taccggacca cgcggcggga agaaagatgg tgtggtgcaa acaatggcga aaagcgccgc
     4081 tcgccaggtg acgaatcaga ttgtacgcgg gatgttgggg agtttgctgg gggggagaaa
     4141 aagataagtc agacgtgatc tgtgcccctc accctaaccc tctccccaaa ggggcgaggg
     4201 gaccgttcga actcgtattt gactttgtca acaataataa agggagcttt cgctcccttg
     4261 ttagattacg attttctcat taacagccac aggctgatta agaagaagct ggcacttggc
     4321 aacagtgcgc cgatgatcgg cgggatgccg taaaccaacg tcagcgggcc gaagatctgg
     4381 tccagtacgt agaagacaaa accgaagctg atacctgtga ccacacgcac gcccatcggt
     4441 acgctacgca gtgggccaaa gataaacgac agcgccatca gcatcatcac cgccacggat
     4501 agcggctgga agattttgct ccacatgttg agctgataac gtccggcatc ctgaccgctc
     4561 gacttcagat acttcacgta gttgtgcaaa ccactgatgg agagtgcatc cgggtccagc
     4621 gccaccacgc ccagtttgtc tggcgtgagg tcggttttcc aggtgccgct caccgtctgc
     4681 gaaccggtaa tctgtttcgg attggtcaga tcagattcat caacctgcga cagacgccag
     4741 actttatgtt ccgggtcaaa cttcgcggta gcggcataac gtacggattg cagacgacga
     4801 ttctcgttaa aggcataaat actgatgcca cctaacacct cgtcaccttt aacccgctca
     4861 atgtagacga agttgttgcc atctttcgcc cataaacctt gctgggtaga gagcaacgag
     4921 ccgccgtaca tcgcctgcgc acggtagtta cgcgccatct gttcgccctg cggcgcgacc
     4981 cattcgccaa tcgccatcgt cagcaagacc agtggaatgg cggttttcat caccgacagc
     5041 gctacctgca tacgggtaaa accagaagcc tgcatcacca ccagttcgct gcgctgcgcc
     5101 agcatcccaa gaccaagcaa cgccccaaga agagccgcca tcgggaagaa gatctgcaca
     5161 tctttcggca cgctcagcaa ggtatacatt cctgcgccta acgcgtcgta actcccctgc
     5221 ccggcttttt tcagctgatc gacaaatttg ataatgcccg acagcgacac cagcatgaac
     5281 agcgtcatca tgatggtggt gaaaatagtt ttaccgatat agcggtcaag tacgccaaaa
     5341 ggttgcatca caccgctcct ttacgcgaaa aactggcgcg caggcggcgg atcggcacgg
     5401 tgtcccaaag gttgagaaca atcgccaaag ccagataaat caggttaacg gtccacatcc
     5461 acagtgtcgg gtccagctta cctttaccgc cgttcgattt cagggaggtc tggatcagga
     5521 agaaaagtag atacagcagc atggctggca gcatcgacag tacgcgtccc tgacgcgggt
     5581 taaccacgct caacggtaca accataagtg ccatcataaa cacggtgaat accaacgtga
     5641 tacgccagtt cagttctgcg cgagcacgat cggtgtcagt gttccacaat gtgcgcatgt
     5701 ccatctggtc ggtatcgttc gggtcgagcg ccaccgcctg gtgaccaatg atcgcctgat
     5761 aatcctggaa atccgtaatg cggaaatcac gtaacagtgc agtgccttcg aagcgtgttc
     5821 cctggttgag agtgacgacc tgggagccgt cgcgcagctg ggttaaatgt ccggaatcgg
     5881 ccaccaccac agaaggacgt gcattacctt ttggtcgaat ttgtgcgagg aacacatctt
     5941 tgaaatcgct gccgtcaacg ctttcgatga acagcaccga gctgccatta gtcgcttgct
     6001 ggaattgccc ttgcgccagc gccgccatgc cagggttcgc tttcgcttct gctaacactt
     6061 catcctgatg acgcgatgac cacggtcccg cccacatcac gttaaccgcc gcgacgattg
     6121 ccgtgaatac cgcaaggatc attgccgctt tcaccagaac cgctttgctc aagccgcagg
     6181 catgcattac cgtaatttca ctttcggtat acagtttgcc cagcgtcatc agcagcccga
     6241 ggaacaggct taatggcagg ataagctgcg ccatttccgg cacgcccaac ccgagaaggg
     6301 agagcaccag attcgccgga atatcgccgt caaccgctgc gccgaggatc ctcactaact
     6361 tttgacagaa gaagatcaaa agcaagatga agagtatcgc cagctggctt ttgagcgtct
     6421 cccgcaccag atatcttatg attatcactt taaatacgcc cgtaaaaact cgtcttttgc
     6481 aggattttag cttgtttcat ggcttaaacg tcatttattc tcttgagtcg tcgaaatcgt
     6541 cgctaagata attatactca acggattcac ctctcagatt ttgttctgac gtgccaatgc
     6601 cgtaataacg ttaagattaa cacgaagtca tcgcaacagc ggacatgagt tacgaaagct
     6661 tgcaattcta tctgtagcca ccgccgttgt ctttaagatt caggagcgta gtgcatggag
     6721 tttagtgtaa aaagcggtag cccggagaaa cagcggagtg cctgcatcgt cgtgggcgtc
     6781 ttcgaaccac gtcgcctttc tccgattgca gaacagctcg ataaaatcag cgatgggtac
     6841 atcagcgccc tgctacgccg gggcgaactg gaaggaaaac cggggcagac attgttgctg
     6901 caccatgttc cgaatgtact ttccgagcga attctcctta ttggttgcgg caaagaacgt
     6961 gagctggatg agcgtcagta caagcaggtt attcagaaaa ccattaatac gctgaatgat
     7021 actggctcga tggaagcggt ctgctttctg actgaactgc acgttaaagg ccgtaacaac
     7081 tactggaaag tgcgtcaggc tgtcgagaca gcaaaagaga cgctctacag tttcgatcag
     7141 ttgaaaacga ataagagcga accgcgtcgt ccgctgcgta aaatggtgtt caacgtgccg
     7201 acccgccgtg aactgaccag cggtgagcgc gcgatccagc acggtctggc gattgccgcc
     7261 gggataaaag cagcgaaaga tctcggtaat atgccgccga atatctgtaa cgccgcttat
     7321 ctcgcctctc aggcgcgcca gctggctgac agctacagca agaatgtcat cacccgcgtt
     7381 atcggcgaac agcagatgaa agagctggga atgcactctt atctggcggt cggtcagggt
     7441 tcacaaaacg aatcgctgat gtcggtgatt gagtacaaag gcaacgcgtc ggaagatgca
     7501 cgcccaatcg tgctggtggg taaaggttta accttcgact ccggcggtat ctccatcaag
     7561 ccttcagaag gcatggatga gatgaagtac gatatgtgcg gcgcggcggc ggtttacggc
     7621 gtgatgcgta tggtcgcgga gctgcaactg ccgattaacg ttattggcgt gctggcaggc
     7681 tgtgaaaaca tgcctggcgg gcgtgcctat cgtccgggcg atgtgttaac caccatgtcc
     7741 ggtcaaaccg ttgaagtgct gaacaccgat gccgaaggcc gcctggtact gtgcgacgtg
     7801 ttaacttacg ttgaacgttt tgagccggaa gcagtgattg acgtggcgac gctgaccggt
     7861 gcctgcgtga tcgcgctggg tcatcatatt actggtctga tggcgaacca taatccgctg
     7921 gcccatgaac tgattgccgc gtctgaacaa tccggtgacc gcgcatggcg cttaccgctg
     7981 ggtgacgagt atcaggaaca gctggagtcc aattttgccg atatggcgaa cattggcggt
     8041 cgtcctggtg gggcgattac cgcaggttgc ttcctgtcac gctttacccg taagtataac
     8101 tgggcgcacc tggatattgc aggaaccgcc tggcgttctg gtaaagcaaa aggcgcaacc
     8161 ggtcgtccgg tagcgttgct ggcacagttc ctgttaaacc gcgctgggtt taacggcgaa
     8221 gagtaagttt gcgttttacc ctcaccctaa ccctctccct aagggagagg gaaccgatcg
     8281 agcgcaatag aaattcattg ctcatctttc agggtgagga atgcagcaat atttaaatcc
     8341 accacaagaa gccccatata tgaaaaacgc aacgttctac cttctggaca atgacaccac
     8401 cgtcgatggc ttaagcgccg ttgagcaact ggtgtgtgaa attgccgcag aacgttggcg
     8461 cagcggtaag cgcgtactca tcgcctgtga agatgaaaag caggcttacc ggctggatga
     8521 agccctgtgg gcgcgtccgg cagaaagctt tgttccgcat aatttagcgg gagaaggacc
     8581 gcgcggcggt gcgccggtgg agatcgcctg gccgcaaaaa cgcagcagca gcccgcgcga
     8641 tatattgatt agtctgcgaa caagctttgc agattttgcc accgctttca cagaagtggt
     8701 agacttcgtt ccttatgaag attctctgaa acaactggcg cgcgaacgct ataaagccta
     8761 ccgcgtggct ggtttcaacc tgaatacggc aacctggaaa taatggaaaa gacatacaac
     8821 ccacaagata tcgaacagcc gctttacgag cactgggaaa agcagggcta ctttaagcct
     8881 aatggcgatg aaagccagga aagtttctgc atcatgatcc cgccgccgaa cgtcaccggc
     8941 agtttgcata tgggtcacgc cttccagcaa accatcatgg ataccatgat ccgctatcag
     9001 cgcatgcagg gcaaaaacac cctgtggcag gtcggtactg accacgccgg gatcgctacc
     9061 cagatggtcg ttgagcgcaa gattgccgca gaagaaggta aaacccgtca cgactacggc
     9121 cgcgaagctt tcatcgacaa aatctgggaa tggaaagcgg aatctggcgg caccattacc
     9181 cgtcagatgc gccgtctcgg caactccgtc gactgggagc gtgaacgctt caccatggac
     9241 gaaggcctgt ccaatgcggt gaaagaagtt ttcgttcgtc tgtataaaga agacctgatt
     9301 taccgtggca agcgcctggt aaactgggac ccgaaactgc gtaccgctat ttctgacctg
     9361 gaagtggaaa accgcgaatc gaaaggttcg atgtggcaca tccgctatcc gctggctgac
     9421 ggcgcgaaaa ccgcagacgg taaagattat ctggtggttg cgaccactcg cccggaaacc
     9481 ctgctgggcg ataccggtgt agctgttaac ccggaagatc cgcgttacaa agatctgatt
     9541 ggcaaatatg tcattctgcc gctggttaac cgtcgtattc cgatcgttgg cgacgaacac
     9601 gccgacatgg aaaaaggcac cggctgcgtg aagatcaccc cggcgcacga ctttaacgac
     9661 tatgaagtcg gtaaacgtca cgccctgccg atgatcaaca tcctgacctt tgacggtgat
     9721 atccgtgaaa gcgcccaggt gttcgatacc aaaggtaacg aatctgacgt ttattccagc
     9781 gagatcccgg cagagttcca gaaactcgag cgttttgctg cacgtaaagc agtcgttgct
     9841 gcggttgatg cactcggcct gctggaagaa attaaaccgc acgacctgac cgttccttac
     9901 ggcgaccgtg gcggcgtggt tatcgaaccg atgctgaccg accagtggta cgtgcgtgcc
     9961 gatgtactgg cgaaaccggc agttgaagcg gttgagaacg gcgacattca gttcgtaccg
    10021 aagcagtacg aaaacatgta cttctcctgg atgcgcgata ttcaggactg gtgtatctct
    10081 cgtcagctgt ggtggggtca ccgtatcccg gcatggtatg acgaagcggg taatgtttat
    10141 gttggccgca acgaagaaga agtgcgcaag gaaaataacc tcggtgccaa cgttgccctg
    10201 cgtcaggacg aagacgttct cgacacctgg ttctcttctg cactgtggac cttctctacc
    10261 cttggctggc cggaaaatac cgacgccctg cgtcagttcc acccaaccag cgtgatggta
    10321 tctggtttcg acatcatctt cttctggatt gcccgcatga tcatgatgac catgcacttc
    10381 atcaaagatg aaaatggcaa accgcaggtg ccgttccaca ctgtttacat gaccggtctg
    10441 attcgtgacg acgaaggtca gaagatgtcc aaatccaagg gtaacgttat cgacccgctg
    10501 gatatggttg acggtatttc gctgccagaa ctgctggaaa aacgcaccgg caatatgatg
    10561 cagccgcagc tggcggacaa aatccgtaag cgcaccgaga aacagttccc gaacggcatt
    10621 gagccgcacg gcaccgatgc cctgcgcttc accctggcgg cgctggcgtc taccggtcgt
    10681 gacatcaact gggatatgaa gcgtctggaa ggctaccgta acttctgtaa caagctgtgg
    10741 aacgccagcc gctttgtgct gatgaacaca gaaggtcagg attgcggctt caacggcggc
    10801 gaaatgacgc tatcgctggc ggaccgctgg attctggcgg agttcaacca gactatcaaa
    10861 gcgtaccgcg aagcgctgga cagcttccgc ttcgatatcg ccgcaggcat tttgtatgag
    10921 ttcacctgga accagttctg cgactggtat ctggagctga ccaagccggt aatgaacggt
    10981 ggaacagaag ccgaactgcg cggtactcgc catacgctgg tgactgtact ggaaggtctg
    11041 ctgcgcctcg cgcatccgat cattccgttc atcaccgaaa ccatctggca gcgtgtgaaa
    11101 gtactttgcg gtatcactgc cgacaccatc atgctgcagc cgttcccgca gtatgatgca
    11161 tctcaggttg atgaagccgc actggccgac accgagtggc tgaaacaggc gatcgttgct
    11221 gtacgtaaca tccgtgcaga aatgaacatc gcgccgggca aaccgctgga gctgctgctg
    11281 cgtggttgca gcgcggatgc agaacgtcgc gtaaatgaaa accgtggctt cctgcaaacg
    11341 ctggcgcgtc tggaaagcat caccgtgctg cctgccgatg acaaaggtcc ggtttccgtt
    11401 accaagatca tcgacggcgc agagctgctg atcccgatgg ctggcctcat caacaaagaa
    11461 gatgagctgg cgcgcctggc gaaagaagtg gcgaagatcg aaggtgaaat cagccgcatc
    11521 gagaacaagc tggcaaatga aggctttgtt gcccgcgcac cggaagcggt tatcgcgaaa
    11581 gagcgtgaga agctggaagg ttatgcagaa gcgaaagcca agctgattga acagcaggct
    11641 gttatcgccg cactgtaatc aacacagtga tgaaaacgaa ggccggagca tgctccggcc
    11701 ttttttatct cttacagaaa tggtaaagaa ggcatgactc cccgagaaat actggctaag
    11761 aagcctttat ctggttgttc ttctttattg attaaaggaa ggtcatccaa atcgcccagc
    11821 aaatacgtat tttttgcctg ccagtcaatc atccatattt tcagcagtgg gtaagccaga
    11881 ccacccgtaa tcccggatat caccaccaac gcacacatgc gataaagcat accgtggtac
    11941 gttaaagttg agcgaaaacg aatacgccca tcattcagtg acaggttgct cataaaatga
    12001 tttcgcaacg acaccgttaa ataactggtg ctaacagcaa tcccaaaata ataaattaac
    12061 tgcgcgatta tcattttacg ttgcatttcc ataaattgct gtaaattctc aatatcatca
    12121 tttgcatacc cagaactatc atactcattt aatatttgat cgaagataat ataaccagca
    12181 acagcgagaa aaggtaataa atctaaaaat gtgaggatgg catattttat acaatacgca
    12241 gtatctaatt tcaccttgaa acgatgtata ccgaaactgg tattgctcca gagaaaactc
    12301 attaccaggc tatataaagt accattaaaa ataccaatgg aaacaatacc aactattgcc
    12361 atcagaacca cagatataat aacactactt gaactattgg catgtagcat ctttgtcgag
    12421 ataaagaaaa cagtccccat cccaatggcc attaaaatcg gcaagaaaaa ggttacccac
    12481 cagaaccctt tcaaagagca attaaaacta aatcttacgc tgttgagact ggtcattaag
    12541 gcctgataac gcagtccttt tgctgccata aatataagca aaaccaacag taataaagtc
    12601 aaaacacagc caacaagcgg catacctgct gacactgtca ttaagattgc gaaatagaaa
    12661 aaaacaaaaa caagacaact aacaaaaaca ttcccaccgg taattccata agaaaatcgt
    12721 tgtccgttaa cttccatatt agcataaaga taacgcttac atttcattaa tgcccatggt
    12781 aaatagatac ctagcgttat aatcgttaac aacacattca ccacacaaat aagaaaatat
    12841 ctctcacctg taccatgaaa aacaaaagaa tgggacggaa catccatttc attaataact
    12901 tgagccataa aaatcccctg aattaaaaaa tccattgata taatttatag atacagacag
    12961 taaaatgttc tgccacgtta caatactctt atcagtaata ggctggcaaa aactttattg
    13021 ctttataaga cgcttgcgca cgtaagaaga gagtggtatt aaacaaatgt ataattactt
    13081 tatttttatt gggttaaact atgaataaca ttgcgccgca atcacctgta atgcgtcgcc
    13141 tgacgctgca ggataatcct gctatcgcca gcgtcatccg tcaggtttac gcagagtatg
    13201 gtcttaccgc cgataaaggc tacaccgttg ccgatccgaa tctcgacgaa ctgtatcaag
    13261 tgtacagcca gcctggccat gcttattggg tggttgaata caatggcgaa gtggtcggcg
    13321 gcggcgggat tgcgccatta acaggtagtg aagcggacat ttgcgaactg cagaaaatgt
    13381 actttcttcc cgctattcgc ggtaaagggc tagcgaaaaa actggcctta atggcgatgg
    13441 agcaggctcg agagatgggc ttcaaacgct gctatctgga aacgaccgct tttttaaagg
    13501 aagccattgc gctttatgag catttgggct ttgagcatat cgactatgcg cttggctgca
    13561 cgggccatgt cgattgtgaa gtgcggatgc tacgcgaact ctaaaaaaat gcccgctagt
    13621 atgtgctatc gggcatttta atcaattaat tagtggcgaa ccccgtcatc gtcttcatcg
    13681 acaaaatctt catcgtcgcc atcttcgccg ttaggatctt caaagtaagt gccccaaccg
    13741 tcgtactcaa cgtcaaattt ctccgccagc gtcatcagtt gttcaacctg ggcatcgatc
    13801 agatcggcat tcaacgcgca ctcgctgagg atgtcgcagc aaatcacgat atcaccatct
    13861 tcaacttcca gctcttctgg atcggtcact tcgtaaccga gtttaaacgc ttcaactgcc
    13921 gctttttcca gagtttccag atcgtctgcg gaaagatggt gttcgatggt gtacagcgcg
    13981 tccggatcgc tgccatcttc cagtaattct tcaataatca
//
LOCUS       DAJTNM010000100        13946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTNM010000100 DAJTNM010000000
VERSION     DAJTNM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.100"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..484)
                     /locus_tag="OO172_003048"
     CDS             complement(<1..484)
                     /locus_tag="OO172_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5513158.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     L"
     gene            complement(515..1207)
                     /gene="fimC"
                     /locus_tag="OO172_003049"
     CDS             complement(515..1207)
                     /gene="fimC"
                     /locus_tag="OO172_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5513159.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1408..1950)
                     /gene="fimA"
                     /locus_tag="OO172_003050"
     CDS             complement(1408..1950)
                     /gene="fimA"
                     /locus_tag="OO172_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5513160.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2431..3297
                     /gene="folD"
                     /locus_tag="OO172_003051"
     CDS             2431..3297
                     /gene="folD"
                     /locus_tag="OO172_003051"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5513161.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3299..3511
                     /gene="ybcJ"
                     /locus_tag="OO172_003052"
     CDS             3299..3511
                     /gene="ybcJ"
                     /locus_tag="OO172_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5513162.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3619..4140
                     /locus_tag="OO172_003053"
     CDS             3619..4140
                     /locus_tag="OO172_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5513163.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4176..5561)
                     /gene="cysS"
                     /locus_tag="OO172_003054"
     CDS             complement(4176..5561)
                     /gene="cysS"
                     /locus_tag="OO172_003054"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5513164.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5735..6229
                     /gene="ppiB"
                     /locus_tag="OO172_003055"
     CDS             5735..6229
                     /gene="ppiB"
                     /locus_tag="OO172_003055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5513165.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6232..6954
                     /gene="lpxH"
                     /locus_tag="OO172_003056"
     CDS             6232..6954
                     /gene="lpxH"
                     /locus_tag="OO172_003056"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5513166.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7072..7581
                     /gene="purE"
                     /locus_tag="OO172_003057"
     CDS             7072..7581
                     /gene="purE"
                     /locus_tag="OO172_003057"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5513167.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7578..8645
                     /gene="purK"
                     /locus_tag="OO172_003058"
     CDS             7578..8645
                     /gene="purK"
                     /locus_tag="OO172_003058"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5513168.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8782..9675)
                     /locus_tag="OO172_003059"
     CDS             complement(8782..9675)
                     /locus_tag="OO172_003059"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5513169.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRWVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9672..10319)
                     /locus_tag="OO172_003060"
                     /pseudo
     CDS             complement(9672..10319)
                     /locus_tag="OO172_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10322..11554
                     /gene="allC"
                     /locus_tag="OO172_003061"
     CDS             10322..11554
                     /gene="allC"
                     /locus_tag="OO172_003061"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5513170.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11565..12350
                     /gene="allE"
                     /locus_tag="OO172_003062"
     CDS             11565..12350
                     /gene="allE"
                     /locus_tag="OO172_003062"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5513171.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(12578..13723)
                     /gene="glxK"
                     /locus_tag="OO172_003063"
     CDS             complement(12578..13723)
                     /gene="glxK"
                     /locus_tag="OO172_003063"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HCR5513172.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDALFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(13745..>13946)
                     /locus_tag="OO172_003064"
     CDS             complement(13745..>13946)
                     /locus_tag="OO172_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCR5513173.1"
                     /translation="QQITFTERNIYRLALPLFVGIFLMALPPVYLQDLPLTLRPLLSN
                     GLLVGILLAVLMDNLIPWERIE"
BASE COUNT         3384 a         3677 c         3495 g         3390 t
ORIGIN      
        1 tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa tcaaagttaa
       61 ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc tgctgggcta
      121 actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg ttaaaacagg
      181 gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat acgatatcct
      241 gtgaaccaat gaactcatca ttacgccaga gatccacccg ataaacgccc gcaggttgat
      301 gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct aataaaaatg
      361 ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca agagatgaat
      421 aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta gtgggtattt
      481 tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact tctctgaccg
      541 gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc actcctgagg
      601 gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac ttttggctgc
      661 ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca ttgatatagt
      721 taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct tgtaattgaa
      781 ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca ttcctgccgt
      841 tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac agagactcac
      901 gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg cttttgggtt
      961 cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg ctgctgtttt
     1021 caatccacga attgaccaga aagagctcat tggtatggct atttttaatc cataccgcag
     1081 tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc cctccggcag
     1141 catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac tttattttag
     1201 tcatcatagt taatacgcct tgtgtattcc tgacggcaaa agattgcttt ggcttcttac
     1261 aacacatcat tcccttttca tttaataaag cggcatgata caaagtgaac gtggtttcga
     1321 tttacattgc caccataccg cttcagacaa ccggtacatt actttgttat aagacgtgat
     1381 atataattca aaacaacgtg gttttgatta ttcgtatctc ataataaaag tcgcgtcagc
     1441 atttgcttgc cctgctgatg cacttgtacc ggtgccttta taacgtgcag aaaaatgaag
     1501 aacgttggtc ccggggatca agttctggtt ggttgagaag ctattcccat caggtttgag
     1561 aattgcggat gtattatcaa gtatttcaat accgacaccc gttgctgttg ttgtatttgt
     1621 actggatgca atggccaata aattatcatt gattgcatca gcctgaccag aaaatgcaac
     1681 ggcagcatta gcggcaacaa caggatcgca gtcgttcaac tgaatggtga atggaattaa
     1741 tgcagaggta ttaccaacag cattgaaaat atcggtacga tattgaccga gtgtgacaac
     1801 ctggtctgcc gagtcagtat tcactgaaca ggcagcattc accagttcgc cttcaaagtg
     1861 aattgtaccg ccatctacaa cggcagcata actacccgta gcggcgaata gtgcggcagc
     1921 cagcgcagac gaaataaatc ttaatttcat atatattcct tcaatctcat ttatcgactc
     1981 cacatccgta tataaccgat tactttattt aagacactga tagtagtaaa attccttttt
     2041 atcctctaag aatgtcttaa ttgaaaatat gcactctatt ctaaaaaata gagagccccg
     2101 ttagatgaat acttccgcgc aaaatatatt caacacaaat atagacctga agcggtaaat
     2161 taccaggctg aaaattcttt ttatattgtc aggtatttct taaattatct taatccttaa
     2221 acaaggaaat aaatcagttc cagatttaca acgccatcat gtacgaaaaa tgaagctttc
     2281 aatctcagcg acctcagcga cggtgcgcct aaccttcgca agaggtcgct tcacgcgata
     2341 aatctgaaac gaaacctgac agcgcgcccc gcttctgaca aaatagacgc atccccttcg
     2401 acctacgtaa cagatggaat cctctctctg atggcagcaa agattattga cggtaaaacg
     2461 attgcgcagc aggtgcgctc tgaagttgct caaaaagttc aggctcgtat tgcagccgga
     2521 ctgcgggcac caggactggc cgttgtgctg gtgggtagta accctgcatc gcaaatttat
     2581 gtcgcaagca aacgcaaggc ttgtgaagaa gtcgggttcg tctcccgctc ttatgacctc
     2641 ccggaaacca ccagcgaagc ggagctgctg gagcttatcg atgcgctgaa tgccgacaac
     2701 accatcgatg gcattctggt tcaactgccg ttaccggcgg gtattgataa cgtcaaagtg
     2761 ctggaacgta ttcatccgga caaagacgtg gacggtttcc atccttacaa cgtcggtcgt
     2821 ctgtgccagc gcgcgccgcg tctgcgtccc tgcaccccgc gcggtatcgt cacgctgctt
     2881 gagcgttaca acattgatac cttcggcctc aacgccgtgg tgattggcgc atcgaatatc
     2941 gtcggtcgcc caatgagcat ggaactgttg ctcgcaggtt gcaccaccac cgtgactcac
     3001 cgcttcacta aaaatctgcg tcatcacgta gaaaatgccg atctgttgat cgttgccgtt
     3061 ggcaagccag gctttattcc cggtgactgg atcaaagaag gcgcaattgt gattgatgtc
     3121 ggcatcaacc gtctggaaaa tggcaaagtt gtgggcgacg tcgtgtttga agacgcggct
     3181 aaacgcgcct catacattac gcctgttccc ggcggcgttg gcccgatgac ggttgccacg
     3241 ctgattgaaa acacgctaca ggcgtgcgtt gaatatcatg atccacaggg tgagtaacat
     3301 ggcgacattt tctttaggta aacatccgca cgttgagctg tgcgacttgc tgaaactgga
     3361 aggctggagc gaaagcggcg cgcaggcgaa aatcgcgatt gccgaaggcc aggtgaaagt
     3421 cgacggtgcg gttgaaacgc gcaaacgctg caaaatcgtc gccggtcaga cagtgagttt
     3481 tgcaggacac agcgtacagg ttgttgcctg atccctttcc cggcctgtat caacaggccg
     3541 gttctctttt cagacttttg ccctctacgc cccgttcatc catgctgata gcatcgacat
     3601 tttgccgaag cgaccatcat gccaaccgtt attacgcacg ccgccgttcc cctttgcatt
     3661 ggtttagggt tgggttcgaa agtcatccct ccgcgtctgt tatttgccgg aatcatccta
     3721 gcgatgctgc ccgatgccga cgtattgtcg tttaaatttg gcgttgctta cggcaatgtt
     3781 tttggtcatc gcgggtttac ccattcgctg gtgtttgcgt ttgttgtccc gctcttatgt
     3841 gtgctgattg gacgtcgatg gttcagggct gggctgattc gctgctggct gtttttaacc
     3901 gtctcgttgc tatcgcacag cttactggat tcggtaacca ctggcggtaa gggcgttggc
     3961 tggctgtggc cgtggtcaga tgaacgcttt ttcgctccct ggcaggtgat taaagtcgcg
     4021 ccgtttgcat tgtctcgtta caccacagcg tacgggcatc aggtgattat ttccgaattg
     4081 atgtgggtgt ggttgccggg gatgctactg atgggaatgt tgtggtggcg cagacgataa
     4141 ccggatgcga aaactcgcat ccggcaattg cgcaattact tacgacgcca ggtggtccct
     4201 tgcgggccat cttccagcac gatccccatc tcgttaagac ggtcacgtgc cgcatctgcc
     4261 gccgcccagt ctttcgcttt acgggcatcc agacgctgtt gaattaacgc ttcaatctca
     4321 gccacttcgc tgtcgtctgc ctgcgcgccg ctttgcagga acgcttccgg ttcttgctcc
     4381 agcaggccca atacagcgga aagtttacgc aggtgagatg ccattgcatt cgccgctgcc
     4441 atatcttctg ctttcagacg gttcacttca cgcgccatat caaacagcac ggaataggct
     4501 tccggggtgt tgaaatcgtc gtccatcgct tcaataaagc gcgcttcaaa cgcttcgcca
     4561 ccggcaggcg caacggtttt atctgtgccg cgcagcgcag tgtagaggcg ctccagcgcc
     4621 gcacgcgcct gcttcaggtt ctcttcgctg tagttcagct ggctgcgata gtggcccgac
     4681 atcaggaagt aacgcacggt ttccgcgtcg tagtatttca gcacatcgcg cacggtaaag
     4741 aagttaccca gcgatttgga catcttctcg cggtcaacca tcaccattcc ggagtgcatc
     4801 cagtagttca catactgacc atcatgggca caggtggact gcgcgatttc gttctcgtgg
     4861 tgcgggaaca tcaggtctga accgccgccg tggatatcaa agtggttacc cagctgcttg
     4921 cagttcatgg ccgaacattc aatgtgccag ccaggacgac ccgcgcccca cggagacggc
     4981 cagctcggtt cgccctcttt cgacatcttc cacagaacga agtccatcgg gttgcgtttg
     5041 tcatcaacca cgtcaacgcg cgcgccagcc tgcagctggt cgagatcctg acgcgacagc
     5101 acgccataag ttggatcggt cgggacgtcg aacatcacgt cgccgttgtc cgccacataa
     5161 gcgtgacctt tggcgatcag ttgttcagtg agttcaataa tttctgcgat atggtgcgtc
     5221 gcgcgcggct ccatatccgg gcgcagaatg ttcaaagcat caaaatcttt gtgcatttcg
     5281 gcaatcatgc gatccaccag cgccacaaag ctttcgccat tttcattggc gcgtttgatg
     5341 attttgtcgt cgatatcggt aatgttgcgc acatacttca gtttatagcc gaggaaacgc
     5401 agatagcgcg caaccacgtc aaaagcaaca aaggtacgcc cgtgaccgat atgacagaga
     5461 tcgtaaacgg tgattccaca cacgtacatg ccgacttccc cggcgtgaat aggcttaaat
     5521 tcctcttttt ggcgtgtcag agtattgaag atttttagca tcgaagattc cgtttagaca
     5581 tgtgtgggta attgagttgc gtataatacc catatttccc gcgcgaatca gcatacattg
     5641 cgtgatgatc cgatcgtgcg gttatgctat aacaccaccc tatatatgac ccgaactggg
     5701 ttgaagcacc aatcaaacgg aacaggatgc aaaaatggtt actttccaca ccaatcacgg
     5761 cgatattgtc atcaaaactt ttgacaataa agcacctgaa acagttaaaa acttcctgga
     5821 ctactgccgc gaaggttttt acaacaacac cattttccac cgtgttatca acggctttat
     5881 gattcagggc ggcggttttg aacctggcat gaaacaaaaa gccaccaaag aaccgatcaa
     5941 aaacgaagcc aacaacggcc tgaaaaatac ccgtggtacg ctggcaatgg cacgtactca
     6001 ggctccgcac tctgcaactg cacagttctt catcaacgtg gttgataacg acttcctgaa
     6061 cttctctggc gaaagcctgc aaggttgggg ctactgcgtg tttgctgaag tggttgacgg
     6121 catggacgtg gtagacaaaa tcaaaggtgt tgcaaccggt cgtaacggta tgcaccagga
     6181 cgtgccaaaa gaagacgtta tcattgaaag cgtgaccgtt agcgagtaat cgtggcgaca
     6241 ctctttattg cagatcttca tctctgcgtg gaagaaccgg cgatcaccgc cggttttctg
     6301 cgttttttag cgggggaagc ccgcaaggcc gacgcgctgt atattcttgg cgatctgttt
     6361 gaagcatgga ttggcgacga cgatcccaac ccactccatc gccagatggc ggcagcgatc
     6421 aaagcggtgt ccgattccgg cgttccctgt tatttcattc atggcaaccg tgattttctg
     6481 ctcggcaaac gctttgcccg tgaaagcggc atgacattat tgccggaaga aaaggtgctc
     6541 gaactttatg gtcgccgggt gttgattatg catggcgaca cgctgtgcac cgatgacgcg
     6601 ggttatcagg cttttcgcgc caaagtccac aaaccctggc tgcaaatgct attcctcgcc
     6661 ctgccgttgt ttgtgcgcaa acgcattgcc gcgcgaatgc gcgcgaacag caaagaagcc
     6721 aacagcagta aatcgctggc gatcatggac gttaaccaaa acgctgtggt cagtgcgatg
     6781 gaaaaacatc aggtgcaatg gctaatccac gggcataccc atcgcccggc ggtgcatgaa
     6841 cttatcgcca accagcaacc tgcttttcgc gtggtactgg gtgcctggca tacggaaggt
     6901 tcgatggtga aagtcacggc ggatgacgtt gagctgattc attttccgtt ttaaaaaacc
     6961 cacaactttg ctgatttcac agccacgcaa ccgttttcct tgctctcttt ccgtgctatt
     7021 ctctgtgccc tctaaagccg agagttgtgc accacaggag ttttaagacg catgtcttcc
     7081 cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca aaagcgactg ggctaccatg
     7141 cagttcgccg tcgaaatctt cgaaatcctg aatgtcccgc accacgttga agtggtttct
     7201 gctcaccgca cccccgataa actgttcagc ttcgccgaaa gcgccgaaga gaacggttat
     7261 caggtgatta ttgcgggcgc aggcggcgca gcgcacctgc caggcatgat tgccgccaaa
     7321 acgctggtgc cggtgctggg cgtgccagta cagagcgccg cactgagcgg tgtcgatagc
     7381 ctctactcca tcgtacaaat gccgcgcggc attccggtgg gtacgctggc gattggtaaa
     7441 gctggcgcgg caaacgcggc attactggca gcacaaattc tcgcgactca cgataaagag
     7501 ctacaccagc gtctgaatgg ctggcgcaaa gcccagaccg acgaagtgct ggaaaacccg
     7561 gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg taacgggcag ttaggccgta
     7621 tgctgcgtca ggcaggcgaa ccgttaggca ttgctgtctg gccggtcggg ctggacgctg
     7681 aaccggcggc ggtgcctttt caacaaagcg tgattaccgc tgagatcgaa cgctggccgg
     7741 aaaccgcatt aacccgcgag ctggcgcgtc atccggcctt tgtgaaccgc gatgtgttcc
     7801 cgattattgc tgaccgtctg actcagaaac agcttttcga taagctccac ctgccgaccg
     7861 cgccgtggca gttacttgcc gatcgcagcg agtggcctgc ggtgtttgat cgtttaggtg
     7921 aactggcgat tgttaagcgt cgcactggtg gctatgacgg tcgcggtcaa tggagtttac
     7981 gcgccaatga aaccgaacag ttaccggcag agtgttacgg tgaatgtatt gtcgagcagg
     8041 gcattaactt ctctggtgaa gtatcgctgg ttggcgcgcg cggctttgat ggcagcaccg
     8101 tgttttatcc gctgacgcat aacctgcatc aggacggtat tttgcgcacc agcgtcgctt
     8161 ttccgcaggc caacgcacag cagcaggcgc aagccgaaga gatgctgtcg gcgattatgc
     8221 aggagctggg ctatgtgggc gtgatggcga tggagtgttt tgtcaccccg caaggtctgc
     8281 tgatcaacga actggctccg cgtgtgcata acagcggtca ctggacacaa aacggtgcca
     8341 gcatcagcca gtttgagctg catctgcggg cgattaccga tctgccgtta ccgcaaccag
     8401 tagtgaataa tccgtcggtg atgatcaatc tgattggtag cgatgtgaat tatgactggc
     8461 tgaaattgcc gctggtgcat ctgcactggt acgacaaaga agtccgtccg gggcgtaaag
     8521 tggggcatct gaatttgacc gacagcgaca catcgcgtct gactgcgacg ctggaagcct
     8581 taatcccgct gctgccgcca gagtatgcca gcggcgtgat ttgggcgcag agtaagttca
     8641 gttaagcagg tgctcatttg attgccggat gcggcgtaaa cgccttatcc ggcttaccgc
     8701 tccggtaccc attgtaggcc tgataagacg cgtcaagcgt cgcatcaggc atggtgcacc
     8761 gattgccgga tgctctcatc actacagcga aatacaggtc cccgcttcgc ccgccagcgt
     8821 ctcttcaatt cgcgataacg ccccaatcca cgcgggttta ctacggcttc tgacataacc
     8881 actcaccgcc gttaccttcg gccccatcga accatcggct ttggcaaatg gcgctaactc
     8941 atccggtgtg gcatggcgaa tggcacgttg ctgcggcgtt ccccagtttt catataccgc
     9001 atcagcatcg gtgaggatca ccagtccatc ggcattaatc tgctcggcga gcaacgcagc
     9061 ggcgagatct ttatcaatca ccgcttcact ccctgcccca tcttccgtca caggcacacc
     9121 gccgccgcca ctgcaaatca ccacatgccc ctctttgagc aacaactcga tggcttcgct
     9181 gtcgagaatt ttacgcggtt gcggagacgc caccacccag cgcaaatatt taccatcacg
     9241 tttcatctgc cagccgtaag ccgcttccag tgcctcttgt tcttctggct gataaaccgg
     9301 accaataaat ttctctggct gcaaaaacgc cggatcatca ggcgaaacct caatgcgcgt
     9361 cagcaccgtc gtaacaggcg gcatctgcgg ctgtgcgctc aagctctgcg ccagcatata
     9421 gccaatcatc ccctggcttt ccgccaccag cacatccagc ggatacggtt ctacctcttt
     9481 ccacgccaga ttctgtaacg ccagcagccc cacctgcggc ccgttgccgt gaacaatcgc
     9541 cagccgataa gaacgggcca ggcgtgccag cgcgggtaca gcactggcga tattgcgata
     9601 ttgattttcc gccgtcagcg cctcaccgcg ctggagtaag gcgttgcccc caagagctac
     9661 aaccagtgtt ttcatggttt tccttgtaat aattgttggc caagccagaa ccccagcagc
     9721 gtatctgccc ctgacgtatg ccccagcgcc agcagcgaat caatcgcaac agcggtacgt
     9781 ttcgggcaac tcagagcatg gacaaagtgc aggagtggcg aggcgaaata tccttgcgcg
     9841 gcataacgta aataactgac gctcaccgct gtggtaacga gttgaagatt gtcggaacag
     9901 gcaaaaaacg ggcgaccggc gcgcgcatct aaagcgccat aataccaggc cgccagcagc
     9961 attccgctca acgtgtcatc atgacttggc gttaatccgg ggcctttacc cagccagtgc
    10021 cgccagtcgg tcttaacgcc attgagcgcg gcctgaaaac agtgacgaaa ctggcgtaat
    10081 tcagcgggca gcggatcgct tgccgccatc gccagtggcc cgaaaagtcc ggtttcctcc
    10141 gcgcgttgca tccatgcagc tgcaagcggt tgaggatgcg aaggcggcgt aatacgcagc
    10201 aaacaataac gctgtggctg tttaaccgtg aaacgcccga ggcgaatccc tttagccaca
    10261 acctgtggtc gttcattgcc gcataatcca ccgcataacg catcgaattg cgtgcggcga
    10321 aattacacat tcccgtcaag ctatagaaga aacgctgccc tggctttcct cttttggcgc
    10381 tgacccaacg ggtgggatga cccgtttact ttattcgccg gaatggctgg aaacccagca
    10441 gcaatttaaa aaaagaatgg cagcaagtgg gctggaaaca cgtttcgatg aagtggggaa
    10501 tttatacggt cgcctgagtg gcaccgaata tccacaggaa gtggttctga gcggttcgca
    10561 tatcgatacc gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc tggcggcgtg
    10621 gctggcaatt gactggttga aaacgcaata cggcgcaccg ttacgtacgg tcgaagtggt
    10681 ggcgatggca gaagaagaag gcagccgctt cccgtatgtc ttctggggca gtaaaaatat
    10741 ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag gaaatagttt
    10801 tgccgatgcg atgaaggctt gcggatttac tctgcctaac gccccactaa ctccgcgtca
    10861 ggatataaaa gcctttgttg aactgcatat tgaacagggc tgtgtgctgg aaagtaatgg
    10921 gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt cgctatacgg tgacgctgaa
    10981 cggcgagtca aaccatgcag gcaccacgcc gatgggttat cgtcgtgata cggtttacgc
    11041 tttcagtcgc atttgccatc agtcgatcga aaaagcgaaa aagatgggcg atccgctggt
    11101 tctgaccttt ggcaaagtag agccgcgccc gaatacggta aatgtggtgc cgggtaaaac
    11161 cacgttcacc attgattgtc gtcataccga cgctgccgtg ctgcgcgatt tcacccaaca
    11221 gttagaaaac gacatgcggg cgatttgcga tgaaatggac attggtattg atatcgattt
    11281 atggatggac gaagaacccg tgccgatgaa taaggagctg gtcgccaccc tgacagaatt
    11341 gtgtgaaaga gaaaaactga attaccgggt gatgcacagt ggtgccgggc acgacgcgca
    11401 aattttcgcg cctcgcgtac caacctgcat gatttttatc cccagcatca acgggatcag
    11461 ccataacccg gcggaacgca ccaatattac tgaccttgcc gaaggggtca aaacgttggc
    11521 actcatgctt tatcaacttg cctggcagaa ataaggagtc ataaatggga tatttaaata
    11581 acgtcaccgg ttaccgcgaa gatttactgg ctaaccgtgc gattgttaaa cacggtaatt
    11641 tcgcactgtt aaccccggat ggtctggtaa aaaatattat tccgggcttt gaaaattgtg
    11701 acgcgacaat cctctccacg ccaaagctgg gtgcctcttt tgttgattat ctggtcacac
    11761 tgcatcaaaa cggtggcaac caacagggct tcggtggcga aggcattgaa acgttcctgt
    11821 atgtgatctc tggaaatatc actgccaaag ccgaaggcaa aacatttgcc ttaagcgaag
    11881 gtggctatct ttattgccca ccaggctcct taatgacgtt tgttaacgcc caggccgaag
    11941 acagccaaat ctttttatat aagcgccgct atgttccggt agaaggccat gcaccgtggc
    12001 tggtttctgg caatgccagc gaactggaac gcattcacta tgaaggtatg gatgatgtga
    12061 tcctgctgga ttttctgccc aaagagttag gttttgatat gaacatgcat atcctctctt
    12121 ttgcaccagg tgccagccac ggttatatcg aaacacacgt tcaggaacat ggtgcctata
    12181 ttctttccgg tcagggggtt tataacctcg acaataactg gatcccggtg aaaaaaggcg
    12241 attacatctt tatgggcgct tattctttac aggctggtta tggcgtaggg cgtggtgaag
    12301 cgttcagcta tatttactcg aaagattgca accgcgacgt agagatttaa gtcataccac
    12361 tcgcctgatt atttttagct atgttggatt ttgccacggt ttataccgtg gctttttttg
    12421 taacattgcc tgatgcgctt cgcttatcag gcctacatga cctctgcaat atatagaatt
    12481 tgcgcgattt tcaggaagga tatagtactc atgccgcatc cggcatgagt actgcgctct
    12541 tatacgaaat aaatcgcatc cgctttaaag gcaagagtca gtttttaatt ccctgaccta
    12601 ttttaatggc gcaggcaata tttcgcgcgc tgttgaagag attggtttca ccgctggcga
    12661 gcacttcggc taaaggtgcc agacgaggca aaatgctgaa tagcgcgtca atgccgtact
    12721 ggtgcaccac ttccacgcca tcacccaata caccagcaat cccaatcacc ggtacattaa
    12781 actgcttcgc caccgacgca acgcccagcg gcgctttacc gcctgccgtt tgcgagtcaa
    12841 tgcgcccttc cccggtgatc accagtgctg ccccctgcac cgcctgcgca agattgaccg
    12901 cattcaacac aatctcaatg cccggtttaa tatccgcatt gagaaatacc gccgccgcaa
    12961 tccccatacc gcccgcagcg ccgccgcccg ccatctgaca gacattaatt tcagtttgct
    13021 gttgtaaaac acgggcgtaa ttttgcagcc cctgttccag ctcttcaacc atctccggcg
    13081 ttgccccttt ttgcgggcca aataccgccg ccgcgccgcg tgcccctacc agcgggttat
    13141 caacgtcaca tgccacttca atatggctgt tcgccagacg cggatcgcat tcctccatct
    13201 caatgcttgc cacgcgcgct aaattgccgc cgttagccgc cagcacctgg ccgtcggcat
    13261 caaggaaacg cacaccaagc gcctgcgcca tgcccatacc gccgtcgacc gtcgcactgc
    13321 cgccaatgcc gagaataata tggcgaatgc cgttatccag cgcatggcga attaactccc
    13381 ccgtaccaaa actggaggcc agcaacggat tacgcttttc aggcgcgacc agcatcaggc
    13441 cacttgctgc cgccatctca atcaccgctg ttttcccgtc tccggtaagg ccataaaaag
    13501 cattcacttt ttcgcccatc ggcccgcaga cttcaagcgt cacgatgttg ccacccgtcg
    13561 cggcgaccat cgcatccacc gtcccttcgc cgccatccgc tatcggcaaa cagatatagt
    13621 gcgcatcggg aaagagggtc gaaaacccgg ctttaattgc ctgacaacat ttttctgcac
    13681 ttaagctctc cttaaaagag tctggcgcaa tgacaatctt cattatcaat cctttttcaa
    13741 caaattattc gatgcgttcc cacggaataa gattatccat aagaacagcc agtaaaattc
    13801 cgaccagtaa gccgttactg agcagaggac gaagcgttaa tggaaggtct tgcaaataca
    13861 caggcggtaa tgccattaaa aatatgccga caaataacgg caatgcgagt cgataaatat
    13921 tgcgctcggt aaacgttatt tgctgg
//
LOCUS       DAJTNM010000101        13897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTNM010000101 DAJTNM010000000
VERSION     DAJTNM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.101"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..247)
                     /gene="csgB"
                     /locus_tag="OO172_003065"
     CDS             complement(<1..247)
                     /gene="csgB"
                     /locus_tag="OO172_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5513174.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKI"
     gene            1001..1651
                     /gene="csgD"
                     /locus_tag="OO172_003066"
     CDS             1001..1651
                     /gene="csgD"
                     /locus_tag="OO172_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5513175.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1656..2045
                     /gene="csgE"
                     /locus_tag="OO172_003067"
     CDS             1656..2045
                     /gene="csgE"
                     /locus_tag="OO172_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5513176.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            2070..2486
                     /gene="csgF"
                     /locus_tag="OO172_003068"
     CDS             2070..2486
                     /gene="csgF"
                     /locus_tag="OO172_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5513177.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSVLQNNSTDF"
     gene            2513..3288
                     /locus_tag="OO172_003069"
                     /pseudo
     CDS             2513..3288
                     /locus_tag="OO172_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3352..3843)
                     /locus_tag="OO172_003070"
     CDS             complement(3352..3843)
                     /locus_tag="OO172_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5513178.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3945..4499)
                     /gene="ycdY"
                     /locus_tag="OO172_003071"
     CDS             complement(3945..4499)
                     /gene="ycdY"
                     /locus_tag="OO172_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5513179.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4523..5260)
                     /gene="ycdX"
                     /locus_tag="OO172_003072"
     CDS             complement(4523..5260)
                     /gene="ycdX"
                     /locus_tag="OO172_003072"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5513180.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5315..6253)
                     /gene="ghrA"
                     /locus_tag="OO172_003073"
                     /pseudo
     CDS             complement(5315..6253)
                     /gene="ghrA"
                     /locus_tag="OO172_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6487..6574
                     /locus_tag="OO172_003074"
     tRNA            6487..6574
                     /locus_tag="OO172_003074"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6521..6523,aa:Ser,seq:gga)
     gene            complement(6724..7005)
                     /locus_tag="OO172_003075"
                     /pseudo
     CDS             complement(6724..7005)
                     /locus_tag="OO172_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7287..8291)
                     /locus_tag="OO172_003076"
     CDS             complement(7287..8291)
                     /locus_tag="OO172_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5513181.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8363..8923)
                     /locus_tag="OO172_003077"
                     /pseudo
     CDS             complement(8363..8923)
                     /locus_tag="OO172_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9008..9205)
                     /locus_tag="OO172_003078"
     CDS             complement(9008..9205)
                     /locus_tag="OO172_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513182.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9217..9708)
                     /locus_tag="OO172_003079"
     CDS             complement(9217..9708)
                     /locus_tag="OO172_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513183.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(9705..10079)
                     /locus_tag="OO172_003080"
     CDS             complement(9705..10079)
                     /locus_tag="OO172_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5513184.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10126..10500)
                     /locus_tag="OO172_003081"
     CDS             complement(10126..10500)
                     /locus_tag="OO172_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5513185.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(10663..10884)
                     /locus_tag="OO172_003082"
     CDS             complement(10663..10884)
                     /locus_tag="OO172_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5513186.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10947..11423)
                     /locus_tag="OO172_003083"
     CDS             complement(10947..11423)
                     /locus_tag="OO172_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5513187.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11439..11912)
                     /locus_tag="OO172_003084"
     CDS             complement(11439..11912)
                     /locus_tag="OO172_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5513188.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12254..13075)
                     /locus_tag="OO172_003085"
     CDS             complement(12254..13075)
                     /locus_tag="OO172_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5513189.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13175..13348)
                     /locus_tag="OO172_003086"
     CDS             complement(13175..13348)
                     /locus_tag="OO172_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5513190.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13468..>13897)
                     /locus_tag="OO172_003087"
     CDS             complement(13468..>13897)
                     /locus_tag="OO172_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513191.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3549 a         3405 c         3339 g         3604 t
ORIGIN      
        1 caatctttgc ccgattgcta ctaccttctt gcgcaacaac cgccaaaagt tttgagcctc
       61 cctgccgtaa ctgagcacta ttattagtcc cagcttgacc aattatggct gcctgattaa
      121 atgaagactt actcaattca tttaccgcga agttatattc tgaattagct aaatcataac
      181 ctgctgcggc tgcaatccca ggcgcaccca gtattgttaa catcataaat aacaatttgt
      241 ttttcatgtt gtcaccctgg acctggtcgt acatttaaga aattaaatca tttcaacttg
      301 gttgttaacg caacctgtat tttgttaacg ctgcgttacg atggaaagta tgtctgcgga
      361 aatattttta ataactcacc cgcgcgttgt atttttcttt ttttgttcat accaaagtat
      421 taaaacccgc gtaaatacgc tgttatctac gcaaaaaata ttttgttttc tcttaaatct
      481 ccgttttccg ctatcaaaaa tcaccagaca gtcattcttc ttgcccgtcg ctgattgctg
      541 cgatatgtct tgcgcacaag ccgtgacaaa cctcgtcttt ttgcagtaaa aaaattgtcc
      601 acggaggtgt ggagaaaaaa caaaaacgtt ttacatgacg aaaggactac accgaaatat
      661 tttttatatg tattattagt aagttagcac catttgtatg attttttaaa attgtgcaat
      721 aaaaaccaaa tgtacaactt ttctatcatt tctaaactta ataaaacctt aatgttaaca
      781 ttttaatata atgagttaca ttttgttaca tgtttaacac ttgatttaag atttgtaatg
      841 gctagattga aatcagatgt aatccattag ttttatattt tacccattta gggctgattt
      901 attactacac acagcagtgc aacatctgtc agtacttctg gtgcttctat tttagaggca
      961 gctgtcaggt gtgcgatcaa taaaaaagcg gggtttcatc atgtttaatg aagtccatag
     1021 tattcatggt catacattat tgttgatcac taaaccttct ttgcaggcga cagctctctt
     1081 gcagcacctt aaacaatcgc tagcaattac aggaaaatta cataatattc aacgttctct
     1141 ggacgatatc tcttcaggct ctattattct tctggatatg atggaagcgg ataaaaagct
     1201 tatccattat tggcaggata ctttgagcag gaaaaacaac aatatcaaaa tattgttgct
     1261 aaatacgcct gaagattacc cgtaccgcga cattgaaaac tggcctcata tcaacggcgt
     1321 tttttatgcc atggaggatc aagaacgtgt tgtcaatggg ttgcaaggcg tcctgcgtgg
     1381 cgaatgctac tttacgcaaa agcttgccag ttacctgatt acacattctg gtaactatcg
     1441 ttataacagc acggaatcag ccctccttac tcatcgggaa aaagagatcc tgaataagct
     1501 gcgtatcggc gcgtctaata acgagatcgc tcgttcgttg ttcatcagcg aaaatacggt
     1561 taaaacgcat ctttataatc ttttcaagaa gatagccgta aaaaaccgga cacaagcggt
     1621 ttcctgggca aacgataacc tcaggcgata aagccatgaa acgttattta cgctggattg
     1681 tggcggcaga atttctgttc gccgcaggga atcttcacgc cgttgaggta gaagtcccgg
     1741 gattgttaac tgaccatact gtttcatcta ttggccatga tttttaccga gcctttagtg
     1801 ataaatggga aagtgactat acgggtaact taacgattaa tgaaaggccc agtgcacgat
     1861 ggggaagctg gatcacaata acggtcaatc aggatgttat tttccagact tttttattcc
     1921 cgttgaaaag agacttcgag aaaactgtcg tctttgcact gattcaaact gaagaagcac
     1981 taaatcgtcg ccagataaat caggcgttat taagtacggg cgatttggcg catgatgaat
     2041 tctaaataaa aaattgttcg gaggctgcaa tgcgtgtcaa acatgcagta gttctactca
     2101 tgcttatttc gccattaagt tgggctggaa ccatgacttt ccagttccgt aatccaaact
     2161 ttggtggtaa cccaaataat ggcgcttttt tattaaatag cgctcaggcc caaaactctt
     2221 ataaagatcc gagctataac gatgactttg gtattgaaac accctcagcg ttagataact
     2281 ttactcaggc catccagtca caaattttag gtgggctact gtcgaatatt aataccggta
     2341 aaccgggccg catggtgacc aacgattata ttgttgatat tgctaaccgc gatggtcaat
     2401 tgcagttgaa cgtgacagat cgtaaaaccg gacaaacctc gaccatccag gtttcggttt
     2461 tacaaaataa ctcaaccgat ttttaagccc cagcttcata aggaaaataa tcatgcagcg
     2521 cttatttctt ttggttgccg tcatgttact gagcggatgc ttaaccgccc cgcctaaaga
     2581 agccgccaga ccgacattaa tgcctcgtgc tcagagctac aaagatttga cccatctgcc
     2641 agcgccgacg ggtaagatct ttgtttcggt atacaacatt caggacgaaa ccgggcaatt
     2701 taaaccctac ccggcaagta acttctccac tgctgttccg caaagcgcca gggcttacaa
     2761 aacctgctta acgagcgcaa ggttattcgt gcggcacaag aaaacggcac ggttgccatt
     2821 aataaccgaa tcccgctgca atctttaacg gcggcaaata ttatggttga aggttcgatt
     2881 attggttatg aaagcaacgt taaatcgggc ggtgttgggg caagatattt tgggatcggt
     2941 gcagacacgc aataccagct cgatcagatt gccgtgaacc tgcgtgtcgt caatgtgagt
     3001 accggtgaga tcctctcctc ggtgaacacc agtaagacga tactttccta tgaagttcag
     3061 gccggggttt tccgctttat tgactaccag cgcttgctgg aaggggaagt gggttacacc
     3121 tcaaacgaac ccgttatgct gtgtctgatg tcggctatcg aaacaggggt cattttcctg
     3181 attaatgatg gtatcgaccg tggtctgtgg gatttgcaaa ataaagcaga acggcagaat
     3241 gacattctgg tgaaataccg ccatatgtcg gttccaccgg aatcctgaca gataaaaatg
     3301 ccggaattaa taatccggca tttactgttt aagcgaaagc gtgagtgccc ctcacgcttt
     3361 atttttgatt tcctgctcac ggtgagccgt ctttgcacta cgcgccgcca gccacaggcc
     3421 gctgtttttc attgcgtaac caaataccag cccaagcgcc agcgaaggta acaccagttt
     3481 ccagtcaccc tgcccggcaa acgtcgcgca tgcgcctata aacgtccccg gaacaaatga
     3541 aagcagcagc tgtttggcct gaatacacat cagaaacgcg acaataccgg ttatgacata
     3601 accgagaatt tccagatgtg gtgccagtgc actaccgtaa ataatgacca tcgcccacac
     3661 cacgccacta agcagggttg cagcggagat cgccagccct ttcagcccac cttgcgggca
     3721 agcaaagtag gccgtacagc ccaggaagcc cgcccagctt agtaaaccca gggaaacagc
     3781 cacccatccc cagataccgg agagaatgcc tgttgtgatt gcaatagaga gaagtatgtt
     3841 catgccgcgc atgatagcag aaaacgcggc catgaatgag atcgagcaca cattttatgc
     3901 aacaacaaat tatctgttgc agttatgagt gacgatgatc acatttactc ttcagaatct
     3961 tcttccagct cgtcccacat tgcactaatg gcatcacggg ttagcggtgc catggtgcgc
     4021 cagaaaggcg tggttgcatg ggcctccact ttgccgagga acgcaccaca ccagggtaac
     4081 agatactcac tgaacagtgt ttccagtgct tcgctctcat cttccgttga ctgatcttcc
     4141 agccaggaag ccgcaagcag caatgtgccg atgtgatcgg ctggcgtatc cgccagtggc
     4201 atccctcgtt cggaaagaaa agcgcgcact tccgcttccg ttgcgtcctc aacccatgcg
     4261 ctacgatatg gcggcacggc acattcatcg ccgataaaca acgcattgta atcggcagag
     4321 acttgcgtca tatcacagct tttctgtaaa cgcgtcagta actcatcctg ctccagtggc
     4381 cagttcgcag ccagtttccc ctcacgaatc agagtaaaca gcggcaccag taaaggatct
     4441 tgcggttggc ggtaatagag cgaacccagc acacgacaga ggatagaaaa ctcgttcatt
     4501 aatatatttc cattactagt aattaaaggt ctgcaaattc cgcaatcggt gccataccgc
     4561 gagattcaag gaagttcagt aagcggcgcg gagaaacatt caaaatgcgc tctggcggaa
     4621 aatctaccgc gtcgaggatt ttaagacact cttcaaattc ccccatggta aacgcggtgt
     4681 gagaatccga gcctaatgcc acccaacctc cagcatcacg taccgctgca gctaccgcac
     4741 gacagttgtc ttcactgccc ttacgtgagt gtaaaaatga ggaattattg atttccagcg
     4801 ccacctgatg tttcgcggct gcttcagcaa cggctttaac atctatttca tatttcggat
     4861 ttccgggatg gcttattata tgcacattgc cgctggcgat agtggcgatc atcgcttgtg
     4921 tattggtcgc tttgtcatgt ggcgcaaaaa ccggctcatg aaaaccggca ataatcagat
     4981 ccagcgagtc aaacatttta ccgctgcagt caatttcacc atcaacattt ttaatgttag
     5041 cttcaatgcc gcgcaggatc cctaccccat caaccactcg cggccagata cgcatgttaa
     5101 tgaagtgcca gtgatgcggc gcatcttcca tatccgggcc atgatcggtg atcgcaaaaa
     5161 gtttaatgcc cttttgtttg gcctgggcaa tgtaatcact caatgtgcta tatgcatgtg
     5221 tgctggcaac ggtatgcata tgaaggtcga cgggatacat aactctctcc tgtattcaat
     5281 tcccgccaag gatagcagga atcctgatgc tttattagta gccgcgtgcg cggtcgactt
     5341 gcccgcagac cctctcccct ttttcgagct gggcaatagt gcgagaaatg tactccacag
     5401 cttcagcggg acgggtaatc gcggcgacat gtggtgttat cgtcacgcgt ggatgttgcc
     5461 agagcggact ttcaggcggt aagggttcac gattaaaaac atccagcatt gcgcctttaa
     5521 ctttcccgct atccagcgcc gcgagcaggt catcttccac aacatgaaca ccacgcgcca
     5581 ggttgaggag atacgcgcca tcaggtaatt tttcgagtaa ttgttgatta ataatgccga
     5641 cggtttcagg ggtattcggt aacaaattaa tcaatacccg acattggctc agaaatgcag
     5701 acagttcttc ccgtccggca aagctttgca cgccaggcca cgatttacgg gttcgactcc
     5761 agcaacgcag cggaaagcgc taggtttgca gactctgagc aactttacag cccagtacgc
     5821 ctgcgcccaa aatgccgatg gtaaaatctt cccgatgata ttcaggcagc ggttgccaat
     5881 gcgaactatt ttgctggatg cgataatcat caaaacgtcg aaaccaatgc agcacctgac
     5941 tgacagcata ttcctgcatt tgctcgccca taccggtatc ttccaggcga aaaagtggaa
     6001 cagaagggtt cagcatttca gggtgtgcct gtagcttgct caaaatagaa tcaacaccgg
     6061 ccccgagtgc gaacaccgct ttaagatcgc gccctgccag catttcaaca ggaggatgcc
     6121 agactaaaac ataatcagca gagtcattat ctccgctttt ccatgccctg actcttgcct
     6181 gaggaatagc tttgcgcagt gcctcaatcc accattgggt atcgaacgtt gggtgataaa
     6241 agatgatatc catactgact cccgaaaagc gttgtgcgaa atttattcgc acttatcgtt
     6301 atgatctaca aaggccacca gcataacaaa tccgtggtcg gtggcaaaaa aagcagattt
     6361 cgcttattaa aaccacacat tgattgaagt ttgaataaac gcgcgattct ttcaaaaagt
     6421 ttgttgacct caggtcatga tttccctaaa ttagcgcccg ttccagcaag acaggaacga
     6481 caatttggtg aggtgtccga gtggctgaag gagcacgcct ggaaagtgtg tatacggcaa
     6541 cgtatcgggg gttcgaatcc ccccctcacc gccagattat aagtgcatgt tattgtttaa
     6601 catgcgcttt tttatttttc cttccagaca gtatgccatc gggtatgcca tctgaaaccg
     6661 gtttatatgc cccgcgatca tatcttatcg gacaatttcc gatctttgag catactggca
     6721 aagctatact gccagcatat caggatagtg ttttgcgata atatcattca gtctgtcaac
     6781 cagttctggt tttctgaacg tgatatgcgc ggagcccttc ttaaagtatc tgatgctgaa
     6841 catctcatct tcatagctct ctttaccctg aacagactga atgtgatcat ccagacgaag
     6901 ggtgatgttt ccccggttgt cggggattgg tttaccgctg aacaggtgca acattctttc
     6961 cagatcggcg agtcggtcag cctgttgtcc ggtgataaga tgaaatctat ggtcaccccc
     7021 gtttttgcaa cactgatttt tgattaatgg ttggcttgct taaatctatc cggcgtctct
     7081 gtgggcaagg atgcccgcgc cccgatgagt tccgcacctg ataatcctaa aaatcccctc
     7141 ggcttcagtg agccatttca tctgttaggt ttttaatcag gtcgatttgc cgttcatgtc
     7201 atcaataatc agtcttcgca aaaccagaaa ggtaacttgt atcttattgt ttaaaataaa
     7261 ctaattgagt tatatcagca tctatatcag gcctgatatt cttttttctg agtagtgatt
     7321 gcccaggcaa ttcgagccag tttatttgcc agagcgcagg taaccacatt cgaatgcttc
     7381 ttgttgagtt gctcccgaac ccactcagca agttttccat gcttgcgttc aagtcgcatt
     7441 atgaatgagc gggcacactg aaccagtaac cggcgtaaat ttttatcacc tcgcttactg
     7501 atacccaaca gagtcgattt tcctccagtg ctatactgcc ggggaacaag tcctgtggaa
     7561 gctgcaaaat ccctgctgca tgagaactgt ttcccgtcac caagttgcga cgatagtaag
     7621 ctggcagtaa tcggaccaac accaggaatt gtcataatgc gctgagcggt atcatcagcg
     7681 ttgattgact gacttaattc tgattccagc tctgctattt gctcaacaag gtaaaagtag
     7741 tgggtatgca acctcaccaa aaggcggctc agatattcgg ggatttcgtg ttcagccaac
     7801 acgagagaca accgttttat tacagcatca cctatcggca ggctgattcc aaattccagc
     7861 aagaaaccgt ggatctggtt ggttgttttc accttatctc tgatgagcga ttctctgacc
     7921 cggtgtaacg ccctcatggc ctgctgggtc tcagttcttg gctggacaaa acgcattgat
     7981 ggtcgtgaag ccgcttcgca tatggcttct gcatcaacaa aatcattctt attacttttg
     8041 acaaacgggc gaacgaactg tggagaaatc agcttagttt catgcccaaa atcagagata
     8101 cagcgagcca taaagtgtgc acccgcacag gcttccatta caacaactga gggcggacat
     8161 gtcgccagaa actgcatcag tttagtacgg gtaaattttt tacgcagaag agccttacca
     8221 gacttgtctt gacaatgaat gtggaaggag tgctttccga gatcgatacc aataagtgtg
     8281 atgttctgca tgatggtctc tccgttaaag ggatttacct taacagcgta ctgctgttaa
     8341 ggtgggggtg accatatcat tatccccacc ggtcccatct caccagattg ttgacgataa
     8401 ttttactgcc aaatttacag gggcaattgg ttttgtaatt ccagttcagc cctctgaaga
     8461 cgttgatcac ccctcgctca aacacctcat ccttattctg gtgcagttgt tcaaatgtgc
     8521 tcagaatgtt cgcttcgctg atttccggaa aattatcgta ctccagtgac cggtaccatg
     8581 tatcacgggc ctgcgtatcc attaatgaca gcatcccgga acgttgcatc aggtcacgcc
     8641 agatttcgcg atcaagattc cgggtaatgg ctttcattgc tgtttcgggt ttctccatca
     8701 gccagcagcc gcagcgaaaa tcctgtttca tggcccagtc cggggcagtt ttacctccga
     8761 tgctttccgt cagcaccgag atttcagcca gttgtcggat aagtgtgtcg atttgcaaca
     8821 gtgtactctt tcgtacagcc agaatgtggc gaatgctggt tgagcagata acgtccgtat
     8881 gcccgttgac cacttccgga cgggagattg tgatatctga cataatcgat attccttttt
     8941 gcagataaat gaaaacgcca gccctgtgca ggctggcgtc agaggaatga agtctggtga
     9001 gtgagcttca ccggttctgg tgcagaagct gcagatgacg cagggtacgg acatcctccc
     9061 gggcctgttc gatacagggc aacagtgctg ctgaatccgt tttatcctca tcgttgtcaa
     9121 agataattcc cgatccgcag tcgatattgt cctgcagcca cgcaatcaga atatccagcg
     9181 ctgtttccgt ggttaatgat ttcatgttgt tcctggttac cagtcgaaag tgggtaaacc
     9241 cggcaggcat tcagcgctgg catccagatg aatgagactg acgccataac gccggatgag
     9301 tgtgacgacc agacgacgga acgtttttga cagccccagg cgtttaagcc gcagaaccgg
     9361 gtatgaccac gcatcggtac gtaacagata tccgctacct gtaaaatgaa tccattctga
     9421 ttcaccgaag tcactggtct ggtgtgacag cgagtacaac caggcgttgt ccgtttccgt
     9481 gatatgtgcg gtactgcagc gtatgccggt aagggtagca aatggtggcg tgcattctgc
     9541 tgaggtctgg tcagattcat caacaacacg aagggagtag ccgttatcgc agaccatgtt
     9601 aatcagttca gcgagctcaa taccatcgac gtcaacgaca atgcgcccta tgttcagtgc
     9661 ctgtacgtta acgctgtcgg cttccagtgt cagggccagt ttcatcattt cgcctccgga
     9721 tacttaccca gggtaatgtt atttaccgtt ctgtaattgt cgcgggtcat caggccggtc
     9781 gcccggcgag cccggaggat atcaatactg tttattaact gagagcgggg acaggtgctg
     9841 aatcccggct ggtcggtacg caccagcgcg tatttttcca cgagaaaatt aactgcatca
     9901 cacagtgaaa tgcctgcctc aatatgctgc tcaatcacac gttcatcgac aaacggtgtg
     9961 tcattcagtg tgaggccata atgctggtcc agcagtcgga tgagcagtgt ctgccagatg
    10021 gtgacgggag acgggcagcg agatacctcc cgtacatgtg tgtcggataa tgttttcatc
    10081 ttaagttctc tgtaactggt agtgctaaag gtgagagctt cggggttatg aggtggttgc
    10141 gggtgctggt gtcggataaa cagccagata aacgtaacca caggagccga gggtgtcggc
    10201 ttcgcaggtc agcccttttg catacagcgt gacggtatgc tgatatcggg gattcagttc
    10261 accgctggtg agcatgagtt ccagttgttt catcagcagc ggaaaggcct ggtccagatg
    10321 gtatgcatcc gcattgctga accggcctct gataccggcg cggtctgcaa ggtaatacag
    10381 gcggttaccc tcttgcacca gacgagcgcc aaaacagggc ctcactgtgc agggaagccc
    10441 ccaccagggg cggtcgtgat tgtcatcggg atgagttatc ccggagagtt tgtctgacac
    10501 gataaaatcc tctctgaaaa gtggttaaga atggtttaac gatgacaggt cagccggcgc
    10561 gctgaccggt ttcggtgaag atgtatgcat tgatgagaat ggcctcatcc acagcggcgt
    10621 ctgaggtcat ctggtcgtac tcgtttcccg ggtgacggaa catcagttca gggtgacact
    10681 catcagccgg acatacgggc caaaactgtc cttacggcgt tctgcaaaca cggcaagaac
    10741 gccgggaata tcctgcactt cacgaccagt atacgcttca gcactgccgt gccagcgata
    10801 cttaccggta cagaacggaa aaagacggga tgcaggatgc tgttggtgaa tacgcatggc
    10861 ttcaccacgg gtgatgattc tcatgatgtg atacctctga agacagaaga taaaagtgaa
    10921 aacaggtgtg atgtggttgt gacgggttaa agcagaccat gttcggcaaa ggagaaaacc
    10981 tggttgccac cgactatcag atggtccggc acccggatat ccaccaggcc cagtgcctgt
    11041 accagacgtt cggtgataag ccggtctgcc ttactgggtg tgacttcacc ggacgggtga
    11101 ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat cacttcccgg
    11161 ggatgaactt ccgtacggtt gatggtgccg gtgaagaggg tttcaccggc aatcagctga
    11221 ttctggttgt tcagatacag cacccggaac tcttcccgct ccagtcccgc catgttcaga
    11281 atcagccatt cccgtgccgc acgggtggag gtgaaggcca cgccgggttc atgaagatgg
    11341 cggtccaggg tttgcagggc ccgcagaatg agactgcgct cgccgggcgt catgtttccg
    11401 ggcagaaagg aaagctgctg cattgtccct gcctccattc agtcgatgat gcgcataatg
    11461 gcgctgcatt ccggatgctg cagggcgtaa tcccgcagcc ggtaataatg gaccgtcatg
    11521 gcataacact ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc agcaatgccg
    11581 gcggcttccg ggctcatttc agcgcggtta ccgttcatgg cattgaacag tacccatgtt
    11641 tcgtcatcat cgtcatccgg ttcgggtgcc ataaatgccc cgccgttgtt cagggtgtac
    11701 agattccaga taccaccgca gtagtcttca cacagacggt ccatccagcc gaagacacgg
    11761 ggttccaggg tgacccactg tggaatgagg ccaaaatgct gcggccagaa gctgatgcgc
    11821 tgttcatcag ggacgggagt ggcaaccagt tgttgcaggc cagtctccgg tgcacaggcc
    11881 gaagcggtgg tgttctgaga caatgttttc atgtatgaaa tccttgtaat gtgaataaat
    11941 gagagggagt aaaggtcggg agctggcaac atcacgttca tcactgaccg tgatgacccg
    12001 gtaatatcag ggatgaatga tattcggctg aaattccgga cagccgtacg cgtaaccgtt
    12061 ccggtcacag acggatggcc agatgccatt gcctgtatac tcagtctgca cagactgatt
    12121 tctccgggac ggtaaagcag acgggtacac caggcgcaca ggtcatcatc agaaatggaa
    12181 cgacaccccg gcagcgcctc cggggcgtcg ggtgattctg gtgcctgaat cacgccttca
    12241 gggacatgct gcctcagcgc aggctctcca gcagcgtttc tgccattacc cacaatgcgc
    12301 ggttgagctt aatatcggtg tcgatgttgt gaatggcacg ggtgtggata cgttttcctt
    12361 ttgcactgcg gccggaaatc ccgcctttca gcatattctc ctggatggtc tgatatgcgc
    12421 tccacaggtc tttaccgtaa tcctccctgc gtcgtggtgt cagaatgtca gcggtggtga
    12481 ctggctgatg ttcgtcacca taacggtaag tcagtgccgc ctgtgccagc gcctggcgtg
    12541 ccggtggcgg caggaccagc gactgcatgg catcacgctt ctcctctatc cggtcaaaca
    12601 cgcccaccac ctcgtaagcc ccttcaataa ctttctccac cacatttccc cggtgtggaa
    12661 cacgcaattc tcccagagac tgaccgcaga ccagtccatt ggtacaaatg gcacgaaaat
    12721 atccgggtaa catctggtag ctggatgaac cgtcatgaga gttgagcaga ataatttcag
    12781 ggacatgctg accggttatc tgtccggccc gccgcagacg cagcatgtgt tttgtgtatt
    12841 cccggcgacc cgggtcacgc acacgtgtct ggcaggcgaa gaatggctga aagccttccc
    12901 gctgcaggct ttccagtacg gtgatggtgg gagtgtacgc atagcgttta ctgcgggaag
    12961 tatgccggtc ttcaccgaaa atactgggaa catagtgcat cagttcttcg tgtgtcagcg
    13021 gacggtcacg gcgtatctgg tttgcagcat aaccaaaacg actggctagt cgcataattt
    13081 gctccttatc ggtggttaag atttactggt gtaataaatg aaaaagccac gtctcccgga
    13141 gaagacgcgg cctgatggac gaaattaatg aggtttattg tctgagaaga ccgtcactgg
    13201 cgaaccttcc ggcgtcagtc tctgcgttgc ggataacccg acggaaatga gttccctggt
    13261 catcgtcgat aaacacattg cggtaagctg ttgtgatgac aacagcctgc tcgcgtgaga
    13321 aagggacttc gggcaataaa cgtgacatac aaccagacct gtatttggtt ccctgaaaat
    13381 ataagtccct gtcatcatgg cagagccgta tttacttttg atatcaataa attaataacg
    13441 cgaagatgaa ccgcataact tacgccttta atttgttttg tttacgtagt ccctgaccag
    13501 gtacgcgaat tcgtgaacgc cagtttcacc aaaccaaatc aggtaaccag ggtcgatgtt
    13561 gtaatagtaa ccaacgactt tatgcgtttt tgggttatag agtgttacga tatagtccca
    13621 gccgcaggcg tgggctttac aaccgccacc ttctacccag gtcgttccgt taatgtttac
    13681 aggatacgca ccaccatcag gaccatccat atagccgtct tccccaaaca ttccgtttaa
    13741 cccaacaggg tttaatgctt tgtcgatagc agctctgtag gtagcgtcac cttcgtataa
    13801 ctcgtacgtt gttggacatg caccaggctg gtcagcccgg gcttttgagt cgcatttaag
    13861 tgtctgattt acagtggcgg cgtttgtgtc aggactt
//
LOCUS       DAJTNM010000102        13807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTNM010000102 DAJTNM010000000
VERSION     DAJTNM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.102"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..454
                     /locus_tag="OO172_003088"
                     /pseudo
     CDS             1..454
                     /locus_tag="OO172_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            514..621
                     /locus_tag="OO172_003089"
     CDS             514..621
                     /locus_tag="OO172_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5513192.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="OO172_003090"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="OO172_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5513193.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="OO172_003091"
                     /pseudo
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="OO172_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="OO172_003092"
                     /pseudo
     CDS             complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="OO172_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="OO172_003093"
                     /pseudo
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="OO172_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="OO172_003094"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="OO172_003094"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5513194.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="OO172_003095"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="OO172_003095"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5513195.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="OO172_003096"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="OO172_003096"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5513196.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="OO172_003097"
     CDS             complement(9943..10530)
                     /locus_tag="OO172_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5513197.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="OO172_003098"
     CDS             10700..11278
                     /locus_tag="OO172_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5513198.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12156)
                     /locus_tag="OO172_003099"
                     /pseudo
     CDS             complement(11395..12156)
                     /locus_tag="OO172_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="OO172_003100"
     CDS             complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="OO172_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR5513199.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGMETDINASKTRQQLNDVVGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
BASE COUNT         3281 a         3873 c         3489 g         3164 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgca cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attagccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc cgccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg agcgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgcaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctatagc agcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc
     4981 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 cactaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac catcattttc ttgagcagat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat actcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaacgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctag cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtactctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgatgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccacgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccataccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatttat cttaatcgtt
    13801 tatggat
//
LOCUS       DAJTNM010000103        13759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTNM010000103 DAJTNM010000000
VERSION     DAJTNM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.103"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            44..1396
                     /gene="celF"
                     /locus_tag="OO172_003101"
                     /gene_synonym="chbF"
     CDS             44..1396
                     /gene="celF"
                     /locus_tag="OO172_003101"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5513200.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1409..2167
                     /gene="chbG"
                     /locus_tag="OO172_003102"
     CDS             1409..2167
                     /gene="chbG"
                     /locus_tag="OO172_003102"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5513201.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2214..4475)
                     /gene="katE"
                     /locus_tag="OO172_003103"
     CDS             complement(2214..4475)
                     /gene="katE"
                     /locus_tag="OO172_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5513202.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIREHVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4658..4921
                     /gene="cedA"
                     /locus_tag="OO172_003104"
     CDS             4658..4921
                     /gene="cedA"
                     /locus_tag="OO172_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5513203.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5198..6014
                     /locus_tag="OO172_003105"
                     /pseudo
     CDS             5198..6014
                     /locus_tag="OO172_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6018..7408)
                     /gene="tcyP"
                     /locus_tag="OO172_003106"
                     /pseudo
     CDS             complement(6018..7408)
                     /gene="tcyP"
                     /locus_tag="OO172_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(7541..8131)
                     /locus_tag="OO172_003107"
     CDS             complement(7541..8131)
                     /locus_tag="OO172_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5513204.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8294..8961)
                     /gene="hxpB"
                     /locus_tag="OO172_003108"
                     /pseudo
     CDS             complement(8294..8961)
                     /gene="hxpB"
                     /locus_tag="OO172_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            9108..9644
                     /locus_tag="OO172_003109"
     CDS             9108..9644
                     /locus_tag="OO172_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5513205.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9685..10545)
                     /locus_tag="OO172_003110"
     CDS             complement(9685..10545)
                     /locus_tag="OO172_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5513206.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPSFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10651..10941)
                     /gene="ghoS"
                     /locus_tag="OO172_003111"
     CDS             complement(10651..10941)
                     /gene="ghoS"
                     /locus_tag="OO172_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5513207.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11042..11971)
                     /gene="pfkB"
                     /locus_tag="OO172_003112"
     CDS             complement(11042..11971)
                     /gene="pfkB"
                     /locus_tag="OO172_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5513208.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12257..13015
                     /locus_tag="OO172_003113"
     CDS             12257..13015
                     /locus_tag="OO172_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5513209.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(13068..13175)
                     /locus_tag="OO172_003114"
     CDS             complement(13068..13175)
                     /locus_tag="OO172_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513210.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13347..>13759)
                     /locus_tag="OO172_003115"
     CDS             complement(13347..>13759)
                     /locus_tag="OO172_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513211.1"
                     /translation="PGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLLKGAT
                     AQGPPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIA
                     IHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
BASE COUNT         3540 a         3173 c         3408 g         3638 t
ORIGIN      
        1 cagatatgcc agatatcagt attctgtact gaagggagaa attatgagcc agaaattaaa
       61 agtcgtcact attggtggcg ggagcagcta taccccggag ttactggaag gatttattaa
      121 gcgttatcac gaattgccgg tcagcgaatt atggctggtg gatgtcgagg gtggcaaagc
      181 taaactggat attattttcg atctctgcca acggatgatt gataacgctg gcgtcccgat
      241 gaagctttat aaaacgctgg attgccgcga agcattgaaa gatgctgatt tcgttactac
      301 ccaactgcgc gttggccaat taccggcgcg tgaactggat gaacgtattc cattaagtca
      361 tggttatctt ggtcaggaaa ccaacggcgc gggcggtttg tttaaaggtc tgcgtaccat
      421 tccggtgatt tttgacatcg taaaagatgt cgaagaactt tgtccgaatg catgggtgat
      481 taacttcact aacccggcgg gaatggttac tgaagccgtt tatcgtcata ccggatttaa
      541 acgctttatc ggcgtgtgta atattccgat cggcatgaag atgtttattc acgatgttct
      601 gatgctgaaa gacagcgatg atttatctat cgatttgttt ggcctcaacc atatggtgtt
      661 cattaagaat gtgctggtaa atggcaagtc gcgctttgcc gaattgcttg atggtgtggc
      721 atccgggcag ttaaaagcat ctggcgttaa aaatattttc gatctgccat ttagcgaagg
      781 cttaattcgt tctctgaatc tgttgccgtg ttcttatttg ctttattact tcaagcaaaa
      841 agagatgctg gctattgaaa tgggcgaata ctacaaaggc ggcgcacgag cgcaggtagt
      901 acagaaagtc gagaaacaac tttttgagct gtataaaaac ccggagttga aagttaagcc
      961 gaaagaactg gaacagcgcg gtggggctta ttactctgat gcagcgtgcg aagtgatcaa
     1021 cgctatctac aacgacaagc aagctgaaca ttacgttaat atcccgcatc atgggcatat
     1081 tgataatatt ccggcagact gggcggtaga aatgacctgt aagctggggc gcgatggcgc
     1141 gacgccacat ccgcgcatta cgcatttcga tgataaagtg atggggctga ttcacaccat
     1201 taaaggcttc gagattgctg ccagcaacgc cgcacttagc ggagaattta acgatgtgtt
     1261 actggcgcta aaccttagtc cgttggtgca ttccgatcgc gatgctgagc tgctggcacg
     1321 cgagatgatt ctggcgcacg agaaatggct gccaaatttt gccgactgca tcgcagagct
     1381 taaaaaagca cattaaccga ggctgattat ggaacgctta ctgattgtta atgccgatga
     1441 ttttggctta agcaaaggtc agaactacgg cattatcgag gcctgtcgca atgggattgt
     1501 cacatcgacg acggcgctgg tgaatgggca ggctattgac catgcggtgc agttgagtcg
     1561 tgatgaaccg agtctggcca tagggatgca ctttgtcctt actatgggca agccactgac
     1621 agctatgccg gggttaaccc gcgatggtgt gctgggaaaa tggatctggc agttggcaga
     1681 agaagatgct ttaccgctgg aagaaattac tcaggagctt gccagtcagt atttgcgttt
     1741 cattgagcta tttggacgca aacctacgca tcttgatagc catcatcatg tgcatatgtt
     1801 cccgcagatt ttcccgattg ttgccaggtt tgcggctgaa gaggggattg cgttgcgcat
     1861 cgaccgtcag ccgctatcta acgctggtga tttaccggct aatctgcgca gttcgcaggg
     1921 attcagtagc gcgttttatg gtgaagagat tagcgaggca ctgttcctgc aagtgctgga
     1981 tgacgcaagc catcggggtg atccttcgct ggaggtgatg tgtcatccgg cgtttatcga
     2041 taatactatc cgtcagagtg cttactgttt cccacggtta acggagctgg aagtgctgac
     2101 atcagcgtcg ttgaaatatg caattgctga gcgtggttat cggttgggta gttatcttga
     2161 tgtgtaagta aggtggtaag gtcactcccc aggggacgtg accttatcca tatttacgcc
     2221 ggaattttgt caatcttagg aatgcgtgac cacacgcggt gttctgccat cagcgttaac
     2281 agttcatcca taaaactacc gtcggtgctg tcagcttcca caatcccttc ttcaccctgg
     2341 tcagctacct tgattgttgc tttaaacttg cgcgcgtctc ccgccagcgc aatcggttta
     2401 aggtgtttgt aggcttccat cagatagtag ttggcatcgc cgttgtcagc gatatccgcg
     2461 atattgccgc aagggacaat gaccgcatcg accgtcagcg aaggtgcacc ggcaaaggta
     2521 gcggctatag gcaacaccgt accgtcatcc gcagtcactt cacccattcg ggagtagagc
     2581 agtttggcat gaacgccttt ggccttcagg gccttgagaa tggcaagaag gtctgccgat
     2641 ctcacttcat cattaagtaa aatcgctacc acgcgacctt tcacatcacc gtcaggaatg
     2701 gcgtacaaac ttaaggatgg atcctttttc agaccgttga cgtccggagg tggggtgata
     2761 ttcagctggt cgtcagtcag ttcgataccg agatttttcg ccaccgcctg ggccagagtg
     2821 agatcaatat gcgccagctg gtcaacaacg tgctcacgaa tatacggacg aacgactttg
     2881 cttaactcaa aactgaaacc atcgacaata tggcgctgct cgaatggcgt ctgacttagc
     2941 cagaacagac gcggatggga ataatattcg ccaaacgatg ggctgcgctc gcgaacttta
     3001 ttgccttcca cgcgctcttg gtatgattca aaaccgccgc gtttcggccc cggtggtgtt
     3061 tcgcgcggcc agttatcgtt gatcgagttc ggttcataat tcgccgggtt agtgtcgatc
     3121 cccatacgat gcatgccgtc acgctggaaa ttatggtaag ggcaggtcgg gcggttaatc
     3181 ggaatctcat ggaaattcgg cccaccaaga cgactgattt gtgtatcggt ataggagaac
     3241 aaacgtccct gcaacagggg atcgttggtg aagtccagac caggaacaat atgacctgga
     3301 tggaatgctg cttgttcgtt ttcggcaaag aagttatccg gattgcgatt gagcaccatt
     3361 ttgccgacac gctgaacggg caccagttct tccgggatca gtttggttgg atcgagaaga
     3421 tcgaagtcga acttgaattc atcttcttca ggaatcaact ggaagcccag ttcgtattcc
     3481 ggaaaatcgc ctgcttcaat ggcttcccac aactcgcggc ggtggaagtc cgggtcacgt
     3541 ccggtgagtt tttgtgcttc atcccaaacg agtgaggctt tacctgccag tggtttccag
     3601 tggaaacgta caaacgttgc cttcccttcg gcattaatca ggcggaaggt gtgaataccg
     3661 aagccttcca tggtgcggta actgcggggg atgccgcgat ccgacatcgc ccacatcacg
     3721 ttgtgcagag tttcaggttg cagagaaaca taatcccaga aagtatcgtg ggcactttgc
     3781 ccttgtggaa ttgcccagtg cggttctggt tttaccgcat gaacaaaatc ggggaattta
     3841 tgcgcatcct ggataaagaa gattggcgtg ttattgccaa cgaggtcaaa aataccctct
     3901 tcagtataga acttggtggc aaaaccacgg atatcacgca cggtatcggc agagccagcg
     3961 ccaccctgaa cggtagagaa cctaacaaat actggggtga ttttgttcgg atctgagagg
     4021 aaatccgctt tggtgatatc gcttaagctt ttatatggct ggaaataacc gtgagctgct
     4081 gatccgcgtg catgaacaat acgttccggg atgcgctcat ggtcaaagtg ggtgattttc
     4141 tcgcgcagaa taaaatcttc cagcagcgtt ggaccacggt taccggcacg cagtgagttt
     4201 tgatcatcgg cgatgcgcac gccctgatta gtggtcagcg cataattttc actgcctttg
     4261 cgtacgtctt ccagagaatt aagtttttcg ttacgtgtat caggggcttt caggctccca
     4321 ggggcggtag gttgtgcacc tggcggtgtt ggttcagccg ctggacgatg agatccgtcc
     4381 tcaggtgcca gtgagtccat ccccggtttc gcttcgctgg aatcgtgtag tggtgactgg
     4441 tgctgatgtg ggttcttttc gttatgttgc gacattgaac tcgtctcctt aatttattac
     4501 tgaaagggcc gctgttttgg tcaaaaaact ctctaactat agaccaccag ccagattccg
     4561 cttcggagac tattctggaa acggtgttat gaaatacacg caaagcggga cgtacagggg
     4621 ctaaatcggc taaactacag tttttctaat ttttaatatg cgtttagtga agccagtaat
     4681 gaagaaaccg ctccgccagc aaaaccgcca gattattagc tatgtcccac gcacggaacc
     4741 cgcgccgcca gaacatgcga taaagatgga ttcgtttcgt gatgtctgga tgctgcgtgg
     4801 caaatatgtt gcgtttgtac tgatgggaga gtcatttctg cgctcaccgg cgtttactgt
     4861 gcctgaatct gcccaacgtt gggcaaatca gatccgccag gaaggggaag tgactgagta
     4921 aggattacat acctcctggt tttgccggga gttacaggac ctgaaattct gcatttcgtt
     4981 gctacattca ttcaaaccac ctgtaacaaa aagctgcctt tgacgagcta ttgctataaa
     5041 taagtgatct tttttcttta attgtttatt tattttaaaa tggttaagtt ttgctaatca
     5101 tgatgagtta tttttgatag ttcatcctgt ggcgtcgctc ttaaacttat attgtaaatg
     5161 gcttagattt aaataagata ataattttgt taacaatatg aggcgggaga tgttttctac
     5221 aaacttaata cagagtaatt atggtgattt aaatattaaa agcctggctt tcgactcttt
     5281 taaagaaagg cttgagtcta ccatggcggc actcactttc tttatatcaa cgggtcaatg
     5341 tgactgcgat gaaatagcag agtcgaactt caatcgtatg attgcctata tgtctaacat
     5401 taactatgat gcctcaaagc taggagcgcc agcacttagt tttgatacgt atctgcagga
     5461 caatgttaaa tatagagtga taattaataa tctttatggc agtgagtttc ggataagaga
     5521 tattaatgag gattttgttg ggatggattt tacctgtgaa ttccgacccg aaaaaatgac
     5581 gaatttgatc aatataaaaa ataggttggt tattcattat ttaaggacta tatactatga
     5641 aaaaaattat atacatcctg ccgggagcat ttttgctgcg attcaaaaaa aatgagagtc
     5701 tcctaaagtt tccttcgata atatcaatgc ttaatatcaa tcttcttttt aatccactaa
     5761 atcttccggg aatgggaagt ggtgtgcttg aagatattat gagtattcca gatagttcac
     5821 ttagaaaacg tttcgggtat gaggttttaa gcttctcact acaggcacat tcattgagcc
     5881 aggaatgtat tgataagttg gacatttttt tcgctgacga tctgtttaaa tatgagtcag
     5941 tatgcatcgc cgcgatggaa catttaaaaa gtaaagcaac ggcccctata caaaacggac
     6001 cgttgccagc ataagaatta acggtgtgcc agttcggcgt cgtcttcgct atccagaatg
     6061 gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagctgtcat cgagccacta
     6121 acgtttagcg ccgtacggcc catgtcgata agcggttcaa cggagattaa cagcgccacc
     6181 agggttactg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt tgcaccacca
     6241 ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt cgcaatccac
     6301 atcgggtcca gcgggttaat gccaaccgta ggcgcaacca tcaccgccag cattgccgga
     6361 tacaaaccgg cgcagccgtt ctgaccaatg gttgcaccga aagaggcggc gaaactggcg
     6421 atggattcag gaacgcccag acgacgcgtt tgtgcttcca cattcagtgg gatagacgca
     6481 gcactggaac ggctggtaaa ggcaaacgtc agcacaggcc ataccttacg gaagtacttc
     6541 agcggactca cgccattaat gcccagcaga atgccatgca cagcaaacat aatcagcaga
     6601 ccgaggtagg acgcgacaac gaaacttccc agtttgatga tgtcttgcaa gttagaacct
     6661 gcaaccactt tggtcattag agccagaacg ccgtaagggg tcaactgcat gacaaggcga
     6721 accagtttca tcacccagct ttgtagggta tcgatagcgg ttaagacgcg ttcacctttc
     6781 ggcgcatcat ccttcagcag tttcaatgca gctacgccga ggaatgcggc aaaaattacc
     6841 acgctgataa ttgacgtcgg attggctccg gtaagatcgg caaacgggtt tttcgggata
     6901 aaggacaaga ccagctgcgg aacgctcaga tcagagactt taccaacata gttactttca
     6961 atggcgttca gacgtgcagt ttctgcacca ccctgaacca gaccttcagc cgtcaaacca
     7021 aacaggtggt gaccagcaca ccgaccagcg ccgcaatcag cgtggtaaac aaaagcgtac
     7081 cgatggtcag aaaactgatt ttgcctaact gagatgcgtt atgcagacgg gcaaccgcgc
     7141 tcagaataga ggcgaagact aacggcataa cgatcatttg cagcagttga acattgccgt
     7201 taccaacgat gttaaaccac tgtacagaat ctttaagtac ctggctgtca gaaccataaa
     7261 tggtatgcag ggcaaggcca aaaaccacac ccatcaccag acccaccagc acttttttcg
     7321 ccagactcca ctgtttatgg cgggtctgag ccagcgcaaa cagcagtaca acgaacacca
     7381 cgatgttcgc aattaatgga aagttcatcc ccgttctcct gatcttatta tttctatcac
     7441 tgcgtagcag tcatagtgtt ggcgcaagat taacagaact gtgagctggc tcttatattc
     7501 aaatggaatt gcttattcca gaaaggtgat ttttgccttt ttagtattca acctgatgct
     7561 taataagccg atgaaactgg atctgcaagg tattgatcat ttgcgatgac caacgaacag
     7621 cgctgttctc gggtaatgaa tggggcatcc ataccgacca gacaaataat gccatgcaga
     7681 taaaacgttc cagttggttg cccttttggg gaaccaggat aggcaagcgg aaacgccagc
     7741 ggcatggcca gagcagggga acaccggcgg gtgtcagcat atcggcaagt atgtggctca
     7801 aataacccag caccattcct tgtagcgcat cagccggaat gaaccagcct tccggaacct
     7861 taaggtaaaa ggttgccagc agcgcaaata ccgccagcag actgtgggta aaaccacgat
     7921 gcccaaaagc gcgggcgatc ggttttgata tccattttaa tcgctgccca agaaacgact
     7981 ttgggtgatc gatgtccggt aacaaacacg tcaggattgc ggaagggaca atatgccacc
     8041 agtcaccctg tgccagcacg ggcgtcagct cggcattttt ggcaaatacc gcacaagcaa
     8101 tagaaaagag aaggtgacct tccgccgtca tgataaaact cactaaactg tcgatacgta
     8161 cagtatagga tttttataca gtgcacggaa gtggtgacgg tgtaactctt tctcttaaat
     8221 tgacgattaa tccgtcagtt gaatgcaaat tcagataaag aaccaggggc agcgtgatca
     8281 ctgcccctgg tcattaaccg agaaggtctt ttgcggtgag ttctgtcagc gatgagagtt
     8341 tgacgtttgc taatacaaaa cgtggatcat tttgcgcttc tggcgcagga acgacgatgg
     8401 aacgcatgca ggctgcttta gaagcgatca tgccatttac cgagtcttcc agcgccacgc
     8461 aggtcagtgg gtcaacgccc agttttgctg cgcagtcgag atatacttgc ggatgcggct
     8521 tgctgtaagg cagtttttca gccgaggcga gggcatcgaa actgtcgcgt aagtcaaaca
     8581 tggtcaacac tttttccagc atatgtagtg gtgacgcgga ggccagtccc accaataaac
     8641 cttgttcttt gcataacgca acagcttcgc gcacgcctgg taataatgga cgtgtctctt
     8701 caaccagtga aatggcacgg gcaataaccc gttctactac ttcctgacgg cttggcccat
     8761 tccacggttg ccgggcgtac aaagatcgac caccatatcg atgcgtaaac ctaaggtgtc
     8821 cggcagctcg ttacgacggg agatatctac ccccaggctt gccatcacat ccagttcggc
     8881 tcgatcccat aaaggttctg agtcgataag taatccatcc atatcaaaaa ttgcagcaag
     8941 aatctgacgc ggggttgaca ttacaacctc tcctttagag cattatgtgt aaggtattaa
     9001 gccgtgctta tgattaagcg cctatcatat cgcctttaaa gaatgggcga aaacagatat
     9061 ctacaggtaa actaagtgaa acgacgcgtc ttaatcaagg agaactcatg acgtatcaac
     9121 aagctggacg cattgctgtt ttgaaaagga ttttgggttg ggtgattttt atacctgcat
     9181 tgatctctac attgatttct ttgctgaaat ttatgaatac ccggcaggaa aaccaggaag
     9241 gcattaatgc ggtcatgctc gactttgctc atgtcatgat cgatatgatg caggcgaata
     9301 cgcctttttt gaatctgttc tggtataact ccccgacccc taattttaat ggtggtgtga
     9361 acgttatgtt ctgggtgatt ttcatcctga tttttgtcgg actggcgttg caggattccg
     9421 gtgcccggat gagtcgccag gcgcgcttct tgcgggaagg tgttgaagat caactcattc
     9481 tggaaaaagc caaaggggaa gaggggctaa cgcgtgaaca aattgagtcc cgtattgttg
     9541 ttccgcacca tactattttc ctgcagtttt tctcactata tatcctgccg gttatttgta
     9601 ttgctgcagg ttatgtgttc ttttctctgc ttgggtttat ttaatcaaaa ggccgcacgg
     9661 agcggccatt atcctcaacc catatcatgc tgctaataat ctatccaatg actgctgagc
     9721 aataaccaaa tgctgaccgc caaataatct tgcacgattt aacagcgtgt agagttggta
     9781 aaccggttga cgttcaagga aatcggcagg tagcggggat actgactgat agccgtcata
     9841 gatttgtggc ggctgttcag tatgcagcgg taacatcgcc aggtcgcact ctcggtcacc
     9901 ccagtagcag gcagggtcga aaatgtacgg gccatccgga cccagcgcac agttgccgga
     9961 ccataaatcg ccgtgcaaca gagaaggctg cggttgatgc gaggccagac gctgctgaat
    10021 atgctcgacg atggcgtcga tattgccgaa agcgatccct ttctctgctg ccagttccaa
    10081 ctgccagcca atccgttgtt cagcaaaaaa cgttgaccag cgacgttgcc aggtgttggg
    10141 ctgtggagtt gtggagagcg agttatcaaa atcgaggcca aattgtggtt ggtcactcca
    10201 ttgatgtaaa cgcgcaattt gctgaccaag aataaatgcg ctatgcgcat ccagcggacg
    10261 aggtgggaga taatccatca ccagaaaact gtagtcacgg tcagcgccta ctgcccaaac
    10321 cttaggcacg gtgacggttt tactacgcga cagtaactcc agttggtcgg cttcggcggt
    10381 aaaactggga agcagttccc tttcatcaca tttgacgaaa aagtcatgtc ctgcatagcg
    10441 caaatgccat gcggcatgga cttctccgcc aggcagttca ttacgcagtt cgatttcgcc
    10501 ttcacctaac tgctcgctca aaagacgact gattgcctgc cacatgatgt ctctcccatg
    10561 ttgtctgcca gatcataagg ttagcgcaag aatgcggtgt aaaaacacga gctaacgcac
    10621 accacagact gattattgcc caaaaaccat ttattttctg ttgctattcc attcctccca
    10681 ggtcgtgatg gtgatttcag gatctttgcc ggttgcactt tgggtaagcc ccgaaaccag
    10741 ttctctaatt tcttcttcac tttgggtact gataagtcca aaagtgttag tccccagctc
    10801 atggatattt ccctcatcat ccgtcatggt gagcaaaaaa ccgtcgcgag tcaggtaatt
    10861 attcaactcg ttaatttcag tcaatgtatc ctcatgcaac atgacggtta tgacataacg
    10921 gacaagatcg ccactggcca tagttcaccc ctttgttatc acatgacttt tgagcatagt
    10981 cggagaaacg cgttgccgac aggttggtgg tgattccccc aatgctgggg gaatgttttt
    11041 gttagcggga aaggtaagcg taaatttttt gcgtatcgtc atgggagcac agacgtgttc
    11101 cctgattgag tgtggctgca ctccccgcag ctacgccaaa acggaccatc tcttcaagag
    11161 aggcattttc tgccagtttc agtgtcatcg cgccgaccat gctgtcacca gcgccaacgg
    11221 tactctggct tttcaccggt ggtggcacca cctgaatgca gttttcacta tcaacaccca
    11281 gcgctccttg tggaccaagg gaaacgacaa cccgtttggc cttgccgcta ttaacgattt
    11341 cctgcgcggc tttgcggaca tcgtctggct gggtgagttc gcgactcacc agcgcactga
    11401 gttctttttg gttaggctta accaactcga tgttaccaat cgccagtgct gcacttaacg
    11461 cttcgccaga actgtcgatg atgcagcgga tcccttggtt ttgcgcagcg gaaatcagtt
    11521 gggttaattt ttccagcttc acacctggcg gcaggcttcc gcttatgacc aggatggccc
    11581 cggattcaat ttccagaact tgctcttcaa gctggcgaaa ctcatcttca tttaatgccg
    11641 cgcctggcat aacaaaacga tactgttcac cgcttgcttc cacatgtacg tgtaaattct
    11701 gccgggtcca gtctttggct tctacagtag cgacggggac attttcatcc gccaacagtg
    11761 aaaccaggtg ttcgccggtc gcgccacccg ccgggaagat cgctgtggca ctgcctccaa
    11821 gatgggcaat ggcgcgggcg acgttgatgc cgccgccccc gggttcgaac accggtgcgg
    11881 tacagcgcag ttttccttcg ggataaattt gcggggtaat tgttgcgcta tcgagagagg
    11941 gcgcaagtgt caacgtatag atacgtacca tcatttcctc ctataggctg atttcagtct
    12001 ggcaccgaat cagcggaaag tgaagaaatt aacaatatga tttattagag ctttatttaa
    12061 aattttgcag ataaatatat ataaataaaa atctgttttc gccacatgct tattcaaaaa
    12121 atgaaataag aatacattgc taagttattc gcgccttttt ataattcgtt acctttccag
    12181 ggacgctaat ctttgatcct taagaaagtt tccgggaccg tgatggtctc ttttttgttg
    12241 tacggactct tgataaatga agcttttgaa gacagttcct gccatcgtta tgttggcggg
    12301 gggcatgttt gcgtcactga atgcagctgc cgatgattcc gtttttactg tcatggacga
    12361 tcctgccagc gctaaaaaac ctttcgaagg taatctgaac gcaggatatc ttgcacaatc
    12421 aggcaatacg aaaagctcct cacttacggc tgacaccacc atgacctggt atggacaaac
    12481 cactgcctgg tcgctgtggg ggaacgccag taatacttct tctaatgatg agcgttcttc
    12541 agaaaaatat gcggcgggcg ggcgtagccg ctttaattta actgattatg actatttatt
    12601 tggtcaggca agctggctga cagaccgtta taacggctat cgagagcgcg atgtgttgac
    12661 cgcgggttat ggtcgtcagt ttttgaatgg cccggtacac agtttccgct ttgaattcgg
    12721 tccaggtgtg cgttacgaca aatataccga caatgccagt gaaacccagc cgctgggcta
    12781 tgcctccggg gcttatgcat ggcagttgac tgacaacgct aaatttacgc agggtgtttc
    12841 ggtctttggc gcggaagaca caacgctgaa ctccgaaagc gcattaaatg ttgctattaa
    12901 tgaacatttt ggactgaagg tggcttacaa cgtcacatgg aactctgaac ctccagagtc
    12961 tgcaccggaa cataccgacc gtcggacaac cttatcgctg ggctacagca tgtaaaatta
    13021 tctgccggac cgtgttatgc ggtctggctg tcttcgtttg tcactgctca gccccaaatc
    13081 attgctgccc aacgtagcaa caacatagcg ccacagattg ccagtagcac caccaccatc
    13141 ttccagtgtt ttttatcaaa gcgtttagtc ggcataccag tgtcctgcat atctcttgtg
    13201 cgtgataacg gagtgtaaca aaagggacag gcatcaaggg attttttgtt attacagccg
    13261 tcgggtggcg ttgtgcacat ggtcactgtt tctgcgtttt atgaaaaaaa taaaattatg
    13321 tatcgatata tatcgtaaat agctggttat aaatacgtct ttatttcatc agcactaaaa
    13381 ctcaggcttt ggctgatgac atttatagca gcataatatg tttctacagt tttataatgc
    13441 ttatgatgaa tggctatatg aacagctgac atgttgtcat gatttttagc ggccaatagt
    13501 gtaaataact gatgttgact aaaagaatgt ttttttgcaa aagcacccag cgtcgatata
    13561 taagataaca cgacgtcttc attacccttg ctcatggcga tgtataatgc agggggtccc
    13621 tgtgctgtag ctccttttaa taaatccatg atgttagctt tgctcaactt gtatttaaag
    13681 gcgatgccat taattttaga gaggaaccgt gtgacacaca aagggtgatt attttccatt
    13741 gcggcgtata atcctggct
//
LOCUS       DAJTNM010000104        13753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTNM010000104 DAJTNM010000000
VERSION     DAJTNM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.104"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(195..1448)
                     /locus_tag="OO172_003116"
                     /pseudo
     CDS             complement(195..1448)
                     /locus_tag="OO172_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1555..2448
                     /locus_tag="OO172_003117"
     CDS             1555..2448
                     /locus_tag="OO172_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5513212.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2583..3803
                     /gene="mlc"
                     /locus_tag="OO172_003118"
                     /gene_synonym="dgsA"
     CDS             2583..3803
                     /gene="mlc"
                     /locus_tag="OO172_003118"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5513213.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3928..4623
                     /gene="bioD"
                     /locus_tag="OO172_003119"
     CDS             3928..4623
                     /gene="bioD"
                     /locus_tag="OO172_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5513214.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4576..5868)
                     /gene="clcB"
                     /locus_tag="OO172_003120"
     CDS             complement(4576..5868)
                     /gene="clcB"
                     /locus_tag="OO172_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5513215.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6027..6641)
                     /gene="dmsD"
                     /locus_tag="OO172_003121"
     CDS             complement(6027..6641)
                     /gene="dmsD"
                     /locus_tag="OO172_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5513216.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6684..7538)
                     /locus_tag="OO172_003122"
     CDS             complement(6684..7538)
                     /locus_tag="OO172_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5513217.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7540..8157)
                     /gene="dmsB"
                     /locus_tag="OO172_003123"
     CDS             complement(7540..8157)
                     /gene="dmsB"
                     /locus_tag="OO172_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5513218.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8168..10438)
                     /locus_tag="OO172_003124"
                     /pseudo
     CDS             complement(8168..10438)
                     /locus_tag="OO172_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(10447..11113)
                     /locus_tag="OO172_003125"
                     /pseudo
     CDS             complement(10447..11113)
                     /locus_tag="OO172_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            11096..12696
                     /locus_tag="OO172_003126"
                     /pseudo
     CDS             11096..12696
                     /locus_tag="OO172_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            13190..13558
                     /locus_tag="OO172_003127"
     CDS             13190..13558
                     /locus_tag="OO172_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513219.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
BASE COUNT         3447 a         3506 c         3518 g         3282 t
ORIGIN      
        1 gtagaataac tgcgcatcag taataaatga cacacagcaa aatgaatccg tttatttggg
       61 tacttataat cctgatgacg ctagatgcgc tgcgggaatt ggctggcgct tcgtctattt
      121 taggatggct attaacgttg gtttgagcag gcaataagtc cggacgggta tttaccgcag
      181 tccggactta tttttcaggc gtgcagacga cgatgcaaac gcgccccgac cagcagagca
      241 atgaccagca tcagagcaat aaatgcgccg acgccgttcc agccatagtt atgccagaaa
      301 acaccaccca gcgtcccggc aatactcgac cccagatagt aactgaacag atacagcgag
      361 gaggcctggc ctttagcgcg ttttgcgcgg gggccgatcc agctgctggc tactgagtgg
      421 gctgcgaaga atccggctga gaagagtaac attccggcaa agatcagcca cagcgagcta
      481 aataaggtca tcagtaaacc aaacggcata actcccgtcg aaaacaacat caccggaccg
      541 cgcccatagc gggtggtcat ggttccggct ttgggtgagc tccatgtacc ggtcaaataa
      601 gcccgcgata ataagccaac cacggcctga ctgacatgct agggggaaag catcaaccga
      661 tagccgatgt aattaaacag cgtgacgaac gaccccatca gcaaaaagcc ttctgcgaac
      721 aataacggta atccccggtc acgccagtgc agacgaaagt tgataaataa cgtcttaggg
      781 cgcagcgaag tcgggcaaaa atggcgtgat tcagggagga ttttccagaa catcaacgcc
      841 gaggccagcg cgaaacaacc gattgccgcc agagcaattc gccagttgaa aaagtccgtg
      901 aagacaccac taattaagcg tccgctcatg ccgccaattg agttgccgct gatatacaac
      961 cccatcaaaa aggccacgaa actggggggg atttcctcgc taagataagt catgccaaca
     1021 gctgccacgc cacttaacga aagcccaatc aaggcgcgca taatcaaaat gccgtgccag
     1081 ctggtcatca ttgtcgaaag taacgtacaa atggaggcca acagtagcgc cgtgaccatc
     1141 actggtttgc gaccaatggc atcggatagc gggccagtaa atagcaaacc gatagccaac
     1201 atcgccgtgg aaatggacag tgaaatacta ctgttcgcgg gcgttaagcc aaactcctgc
     1261 gaaagcaccg gaaggatagg ctgcacacaa tagagaagtg caaaggttgc cagtccggca
     1321 gagaacagcg ccagggtgac gcgcataaat tgcggcgtac cgcgtttaat aaattgattt
     1381 ggctgagaaa tgctttgctt gtcagtgtcg cttgccggag cgccatcaac agttgtagta
     1441 cggctcactt gaaatccttg ctaaatatgc ctgtggatca ggcttataca tagggtagga
     1501 aaatcgaatt gttctgtcta atatattaat aatctcaaat aagatgtttt aaatatgaat
     1561 attgaacttc gtcatctgcg ttactttgtt gctgttgcgg aagagctgca ttttgggcgc
     1621 gcggctgccc gcctgaatat ttcgcaaccg ccgctaagtc agcagattca ggcactggag
     1681 caacaaatcg gtgcccgact gctggcacga actaatcgca gtgtattgct gacggcagca
     1741 ggaaaacagt ttcttgcaga tagtcggcaa atcctgtcta tggtggatga cgctgccgct
     1801 cgtgctgaaa gactgcatca gggtgaagcg ggggagttgc gtattggttt tacttcgtcc
     1861 gctcctttta ttcgggcggt gtccgatacg ttatcgctgt ttcgccgtga ttatcctgat
     1921 gtccatttac aaacccgcga aatgaacact cgcgagcaaa tcgctccgct cattgaagga
     1981 acgctggata tgggattgct gcgtaacaca gcgttaccgg agacgcttga acacgcagtc
     2041 atcgtccatg aaccgcttat ggcgatgatc ccgcacgatc atcccctggc aaataatccg
     2101 agtgtaacac tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg
     2161 actgggctgt atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc
     2221 actcaggagg tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt
     2281 tcaattttgc ctgcgtcatt taaacgtgtt cagctcaatg aaatgcgctg ggtgccgatt
     2341 gctgaagagg atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt
     2401 ccagctgcgc gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag
     2461 cgaaaaagcc cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt
     2521 attgaagtgc ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg
     2581 cggtggttgc tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg
     2641 cggtttatcg cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg
     2701 cgcagctggc tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg
     2761 tgcgagagct ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg
     2821 ttgaaactga agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg
     2881 ctctgcgcga tctgagcagc aaactggtgg tggaagagtc gcaggaattg gcgttaaaag
     2941 atgacttgcc attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc
     3001 agaaaaaact tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg
     3061 aaaatggtat tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg
     3121 aggcgctgga gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga
     3181 cgatggcaga ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta
     3241 tcgatcacaa cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca
     3301 gtagtctcgt ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg
     3361 ggaaccacgg ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc
     3421 tgcgtctcaa tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat
     3481 tgtgtcaggc ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg
     3541 cgcatgtagg gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga
     3601 ttggctcacc gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc
     3661 gtcagcaggc ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta
     3721 accagggcac gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt
     3781 tgattcgtct gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc
     3841 aatttgggcc aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat
     3901 ttgtgaagca taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac
     3961 acttctgtag ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga
     4021 aaaacggttg cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg
     4081 cgtaataaag atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca
     4141 gttaatccta tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat
     4201 tacaccctca tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta
     4261 gaagggactg gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta
     4321 gtgcaggaac aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat
     4381 gcactgctaa cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct
     4441 aaccgaatca acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa
     4501 cttccggcac cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg
     4561 gggcaataca tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg
     4621 taacgtccgc gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg
     4681 atactccccg gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc
     4741 cgccaacagt gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg
     4801 gaaccataat cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt
     4861 cggtgtaaag acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt
     4921 acaaaggaag atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac
     4981 ggtgctatag ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa
     5041 cccgcctagt gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca
     5101 ggcgttcatt aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat
     5161 aatcagcgca tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc
     5221 gtcgctatga ttaatcagat tgctaaccag caatgccacg acggcggaaa taatcaccgg
     5281 gccgagagag gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc
     5341 aagcggggca cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt
     5401 ccattcctgg cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag
     5461 aatcatcgca ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag
     5521 cgatttaacc aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta
     5581 atcggtcggc gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc
     5641 cgccgccagt ccgccgagcg ccggagttag cagccgtcgc cagggggaaa ggtttgtcgc
     5701 tgcattgacc agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc
     5761 atgacgaaac ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag
     5821 acggcggaac atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg
     5881 gcaatgaaac acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg
     5941 ctttaagaga taaaaaaacg tgacattgta acgtattatt gccgaatcgc gcgtaatcgt
     6001 caccatccgg caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc
     6061 aacagttgtg attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag
     6121 aaagggtgtt ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg
     6181 tgccatgcca gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc
     6241 atcaacagca gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg
     6301 cctttctcgc gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg
     6361 cgatccagcc agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa
     6421 cgctgccagg cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc
     6481 gctaatgacg cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca
     6541 accagaggtg cggcttccgc actctctgga gcgtaataaa acagcgctcc caacacgcgc
     6601 gccgcgacag aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt
     6661 gcgggttgcc ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg
     6721 ccataaaaga gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca
     6781 agtccggcaa cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct
     6841 gcgcataaca ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg
     6901 gcttgttgca cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg
     6961 gcacaagcga tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg
     7021 cgggctgccc gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag
     7081 aaagccagcg tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag
     7141 gtcatcatcc agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc
     7201 aacgcttgcg gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa
     7261 aatatggaac cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag
     7321 ttaaacgctc gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc
     7381 aacagccaga ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg
     7441 gcgaaccagc caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta
     7501 aagataacca gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc
     7561 cagataacca gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt
     7621 gggttttgtg aaatgcgcgc gcggcagtgg cgcgacggca gccagagtac cgtgcttctg
     7681 gcgcagctct tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat
     7741 gggttgtttc ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt
     7801 ttcagcattg tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat
     7861 acaaacatct tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg
     7921 gcagactttt gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc
     7981 gaacacgttc tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata
     8041 aatacggcgg aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca
     8101 ggttttacag ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg
     8161 ctactcctta aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcta
     8221 gcggtgaagg gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac
     8281 caaacatatc cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac
     8341 gcggagtcac ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat
     8401 cgccatgacg gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc
     8461 aggcctgctg taacacatca atattgccgt agctggagtg ggtacgcgct ttgtagtgga
     8521 agcccgttaa ctgcagtgga taggttttcc gcaggggagc gtcccagcca tcaaaacctg
     8581 aggtatacgc aggaatggga tgaataattt catctttttt caattcccag gtatctgcaa
     8641 tcttcgccag tcgttcagaa taaatttcga ttttccccga aggtgttttc aacgggtttg
     8701 cctgtggatc ttcacggaat gcgcggaaag cgacgtagtg ttcttccggg cattttttct
     8761 taaagatccc ggtcgttttc atctcctcgt agtcgggcat ctcagggtta cgttccttcg
     8821 ttttcgcatg gagatatttg atccattcat gctgactgcg accttcagta aaggtttgat
     8881 aaacgtctgg tcctaagcgt ttggcgactt cactcagcat ccagtagatg ggtttgcgtt
     8941 caaattttgc tgaggttgcg ggttgggcga ggatcacata gcccatattc cctgcagatt
     9001 catgagagat aaggtcttct tgctctgttg gcatcaggtc aggcaacagg atatcgcaat
     9061 acttagccga ggccgtcatg aagtggtcaa tgccaacaat catctcgcac ttgctgtcat
     9121 cctgaagcac ctcatgggtg tgattgatgt cgccatgttg attgatcaat gtgttactgg
     9181 cgtagcacca taaaaacttg atggggacat ccagtttttc ttttccacga acaccatcac
     9241 gggtcgcggt cattttcgta ccatggtcga tggcatctgt ccatgtaaaa acggaaatct
     9301 gcgttttaac aggattctcg agcatcggga accattctac ccccagatcc cagctacctt
     9361 tgcgtacacc tgagttgccg ccgtttatgc cgacgttacc ggtaagaacg gaaagcatgg
     9421 caatagcgcg ggacgtttgt tcgccgttgg agtgtcgttg tggcccccaa ccctgacaaa
     9481 tataagcagg ttttgctgaa ccgatctctc gtgccaactg gataattttt tctgccggga
     9541 tgctggtgat ttttgctgcc cattccggcg ttttagctat gccgtcaggc ccttcgccca
     9601 gaatataggc tttataatgc gcgttacgtg gtgcgttggc gggcagcgtt ttttcatcgt
     9661 aaccaacaca atatttgtcg agaaatggct gatcgaccat gttttcagta atcagtaccc
     9721 aggcaatcgc acaggccagt gcgccatcgg tgccagggcg aatgggcagc cattcatctt
     9781 cacgccctgc agctgtgtcg ttatagcgtg gatcgatgac gatcatgcgt gcgtttgaac
     9841 gttcgagggc ttgctcgacg tagtaagtga caccaccgcc gctcatccgc gtttctgccg
     9901 ggttatttcc gaacataacg accagtttcg tattggcgat atcatccggg ctgttgccat
     9961 cattggcacc gaacatataa ctcattgcgg cactgatctg tgcggtactg tagctgccat
    10021 agcgactgag aaaacccccg caagagttca tcagacggta cgggacgttc gagttggtga
    10081 tatttccgcc atctacgcct gttccgtaca ggacatgtac agcctcattg ccgtaatctt
    10141 tcaggatccg ccgaagatta tcactgatgg tatccagggc ttcgtcccaa cttatccgtt
    10201 caaatttacc ttcaccgcgc tcgccgacgc gcttcatggg atatttcaac ctgtcaggat
    10261 gattcatccg tcggcggata gagcgcccgc gtaaacatgc ccgaacctga tgattaccgt
    10321 aaacgtcgtc acctgtcgta tcagactcca cccagtacac ggtgtcatct ttcacatgca
    10381 aacgtagcag acagcggctc ccgcagttaa cggtgcagga actccagacc gttttctctt
    10441 ctacccatct ttttccagtt cccaggtacg ggcaatttcc gccagcttgc tggaataaat
    10501 ttcaatctta ccggaaggcg ttttaagtgg atttgcctcg gggtcgtcac gaaatgcttt
    10561 gtaggcgaca aaatggccat tgggatcttt acgcttatag atacccattt ttttcagttc
    10621 gtcgtaagac ggtaacgccg gatctttggc aagcattttg gcgtacagat gttgtaacca
    10681 ttgttcctgc gtgcgacctt ctgtgaactt ttgatagacg tcaggtccaa gacgtttcgc
    10741 gacttcactc aggatccagt aaatcggttt acgttcgaat ttttcgctgg tgactggctg
    10801 gaggaaaatg agatatccca tgttaccggc gtagtcgtta ggaataatat cttcctgctc
    10861 aacggtcatc aggtctgcca gcagaatgtc ggcatatttt gccgtatgag gtcataaagt
    10921 tttcgatgac cacaatcatt tcgcatttcg attcgtcctg cagaatttca tgcgttttgt
    10981 tgatgtcaga atgctgatta acgagggtat ttcccgcgta gttccagatg gactccactg
    11041 tcagacggat ggatggactt gaagtagttg actcaacgct acttaaggtc ctgatatgac
    11101 tgacattaac catgataata ctgtatatgc atccagttct ttctctgggt ctccctatct
    11161 gtatcagcaa gtctacacct caagtcctga cacaaggctg acacaaatca aggaccttgc
    11221 agtcccgcac ttgctcgcaa ggctcgctac gccacagtta gtccgtagag ctaacggtcg
    11281 atatactatc cgcttcagac tcaagggaca gaccacaccg tttctttcag tatcgacccg
    11341 cagcacagac aggagagtag ccacaatgcg tcagagagag cttgcagcca ccgctaaggc
    11401 cttcatgttg gataggcctg aagtctcact acaggagctt acagagcatc tccggtctat
    11461 ggctgagcag ttcctgacag acgctagtga cgattactgg aatggacttg aggtcgctac
    11521 tttggtggac gagaagtcca acctgaggga gctagccgct acgcaagcct tgagtctgga
    11581 ccagcagaag ggcataaggt tagcgcttga agtcctgacc gcagcacagc agagggtaga
    11641 cactggtgat acctctggtc tgattaagct gattgatgat aataaccata ctgattactc
    11701 aactataggc gactctacgt caattctgaa gaatgagcag ggggatagcc agcagttttc
    11761 acacaggagc gccaaccatc tgtagttttt tcaagcctag tgtcctcgct gctggctgag
    11821 aaggcccaga ccctgaagtc atccagctac aaggacctgt cgtcctcact gaataccgtc
    11881 tctcggttct tacctgaaga catggacctg atgtcccgct ctgagtggct ggctgtcagg
    11941 gacgctatgc tggcttctga ggtgagaccg tcgacgatta acaagctgct gactaaggcg
    12001 aagatgtgtc ttgattacgg tctgatgaat gggcaacttg agggccgcaa ccctattgaa
    12061 cggatgaagc tgaccaagga tatcgactcc aagcgcagag cgttcacaga cgaggagctg
    12121 gagagacttc tggtccgtgt tgaggctgag tatcagttca cacgacacac ggctcacacg
    12181 acctctgagg cccgtagatg ggcgtctctg gtttctgtag ttactggcgc tcggtctgct
    12241 gaggtctgcc acctgactaa acgtgacatt gtaaccttag acaatggact ggtctgtatc
    12301 gatataaacg aagatgggga cggtaagtct gtgaagaaca agcattcagt ccgacttgtg
    12361 ccgctgaccg atggcgttca tgggttcgac cttgagtctt tcctcaaatg ggtagacatg
    12421 cagcctgatg atggtcctct cttcgggatg actccgagcg cctactctag ctggtttaac
    12481 tcaagggtcc tgactgaggc cctaggagac tctcaggatg tctctctcca cagtctgaga
    12541 cattggctgg cgacccgaat gaaagagcgt ggagtcaatc tggtggacgc tcaaggcatc
    12601 ttaggtcaca gcagtcagtc aattacgtat gacctgtacg ggaaaggaca cgctgtgggt
    12661 aggctggctg atgtgcttaa gacagcttta ctttaagtag ggagacccaa ggtggagacc
    12721 atatacggct tgatggttga gaccttgggt cactgaagtc tgactgaagt tagggagggt
    12781 gataataatc ataatccctc ctgactatag gcccactggt ccgccttcgg ctgtccgact
    12841 ctaagtctgt ctgtggtctt atttaaactg cgtcctaaac tccagtccct ccaaattcct
    12901 atggtctcct cttgacattc ctgtttaact gtgagtcgcc tctggtctct ctttatatat
    12961 acttccttaa ttcctgaggc gtctttagtc cttgacattt ggttcaacct gtgagtcacg
    13021 tccggtcccc ctctttatat ttacttcctt aattcctagg gtcgccttaa tccgtgacat
    13081 ccagtccagc ctgtgagtca catcctgtcg cgctactgta tgcatcacca gtgatggact
    13141 tgattatcac tataggggaa ccaactaatt caactacagg agatactcta tggcacgacc
    13201 aatcgcaact cacgacaaca cgttcaccaa agcctacttg cagcagcact gcggggacct
    13261 gctgtcattc gatggtcagg gcgacctgag tggttggctg gatgatgtcc ttactggtgc
    13321 tggtcgcctg aacgaatcaa tggcaagcaa taccaagcct gtcagcccat acttgattct
    13381 gacccaacta ctaacgcacg acacattaac tgtaagcgct gtgcaggagt ccctgagccg
    13441 taagcgtgtt gccttgggtg aacctatggt ttcaactcgc tacgctcgct acgtctatgc
    13501 taccgtcgtc agcgccagta aatccgtcca gtaccacgcc agtaaagctg gttcatgatt
    13561 acggacccta aactatcact atagggagac cagcctccgc ctctgagtct gatgcgtcag
    13621 aacatgcgag catctggtca accgacatta agtatctctc ctaagtaatc aaggcatgtg
    13681 accgcctctg tcagacctcg tgtatcgacc tccagctcgt tagcgttctt cagtcatctc
    13741 agttagtagc tcg
//
LOCUS       DAJTNM010000105        13668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTNM010000105 DAJTNM010000000
VERSION     DAJTNM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.105"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            8..1261
                     /gene="kch"
                     /locus_tag="OO172_003128"
     CDS             8..1261
                     /gene="kch"
                     /locus_tag="OO172_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5513220.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1316..1489)
                     /locus_tag="OO172_003129"
     CDS             complement(1316..1489)
                     /locus_tag="OO172_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5513221.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1632..3092
                     /gene="cls"
                     /locus_tag="OO172_003130"
     CDS             1632..3092
                     /gene="cls"
                     /locus_tag="OO172_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5513222.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3127..3456
                     /locus_tag="OO172_003131"
     CDS             3127..3456
                     /locus_tag="OO172_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5513223.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3509..4513)
                     /gene="oppF"
                     /locus_tag="OO172_003132"
     CDS             complement(3509..4513)
                     /gene="oppF"
                     /locus_tag="OO172_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5513224.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4510..5523)
                     /gene="oppD"
                     /locus_tag="OO172_003133"
     CDS             complement(4510..5523)
                     /gene="oppD"
                     /locus_tag="OO172_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5513225.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGAATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5535..6443)
                     /gene="oppC"
                     /locus_tag="OO172_003134"
     CDS             complement(5535..6443)
                     /gene="oppC"
                     /locus_tag="OO172_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5513226.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6458..7378)
                     /gene="oppB"
                     /locus_tag="OO172_003135"
     CDS             complement(6458..7378)
                     /gene="oppB"
                     /locus_tag="OO172_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5513227.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7464..9095)
                     /gene="oppA"
                     /locus_tag="OO172_003136"
     CDS             complement(7464..9095)
                     /gene="oppA"
                     /locus_tag="OO172_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5513228.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(9833..10480)
                     /gene="ychE"
                     /locus_tag="OO172_003137"
     CDS             complement(9833..10480)
                     /gene="ychE"
                     /locus_tag="OO172_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5513229.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            10957..13632
                     /gene="adhE"
                     /locus_tag="OO172_003138"
                     /gene_synonym="adhC"
     CDS             10957..13632
                     /gene="adhE"
                     /locus_tag="OO172_003138"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5513230.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3573 a         3397 c         3195 g         3503 t
ORIGIN      
        1 ggtatcagtg agtcactggg ctacattcaa acaaacagca acaaatttat gggtgaccct
       61 acggcacgac attctcgcgc tggccgtctt tttaaatgga ttgcttattt ttaaaacaat
      121 ctatggtatg tcggtcaatt tgcttgatat tttccatatc aaagcatttt cagagttgga
      181 tctctccttg ctggcaaacg ccccactatt tatgctcggc gtctttcttg tcctgaactc
      241 cattggctta ctgttccggg caaagctcgc atgggcaatc agtatcattt tgttgttgat
      301 agcgctaatt tacaccctgc atttttatcc ctggctgaaa tttagtattg gattttgcat
      361 ttttacgctg gtgtttttgc tgatactgcg caaagacttc tcccacagta gcgccgcagc
      421 cgggacaatt tttgcattta ttagtttcac gacattactg ttttactcaa cctacggtgc
      481 actttattta agcgaaggtt ttaatccgcg aatagaaagt ttgatgaccg cgttctattt
      541 ttcgatagaa accatgtcaa ccgtcggcta tggagatatt gtccctgttt ctgaatcagc
      601 acgattgttc actatttcgg tcattatttc cggcattacc gtttttgcca catctatgac
      661 ctcaattttt ggtccgctta tccgcggggg attcaacaaa cttttaaaag gaaacaatca
      721 tacaatgcat cgtaaagatc attttattgt ttgcggacat tcgattctcg ccatcaatac
      781 gatcctgcaa ctgaatcaac gcggacaaaa cgtaacggtt atcagcaact tgcctgaaga
      841 tgatatcaag caacttgagc aacgcttagg cgataacgct gatgttatcc ccggtgacag
      901 taatgacagt tcagtattaa agaaagcggg aatcgatcgt tgccgggcca ttctggcgct
      961 gagtgataac gatgcagata acgcgtttgt tgtactctcg gcaaaagata tgagcagtga
     1021 tgtcaaaaca gttctcgccg tcagtgatag caaaaacctg aataagatta agatggtaca
     1081 tccggatatc attctctcac cgcaactgtt tggcagcgaa attctggcgc gagttttaaa
     1141 tggtgaagag attaataatg atatgctcgt ttcaatgttg ttgaactcag gtcatggtat
     1201 tttcagcgat aacgatgaac aagaaacgaa agctgacagt aaagaatcgg cgcaaaaata
     1261 gagtttaacg acggaagagt aaacttattg ggcaccgcaa tggtgcccag tacatttaga
     1321 tattgtagat cgcaaacaat aacgagttac gctgtttgtt tagaatgcac ttcctgatgc
     1381 tgtggatacg catatttcgg cgcgattgcc cctcaagcca acgcgattta cgtcggctag
     1441 cctgacgctg catgcgccag cgccccactt ccgttctact ccgcttcatg ttaactactc
     1501 tatgcaataa cagaatggtc attatacccc cgcccccgac gctgaccagt gcttttcccg
     1561 cgtttttatt tgccagatga atccatatgc gtaaactcat aacaatgcgc tttcaaaagg
     1621 atttctaatc tatgacaacc gtttatacgt tggtgagttg gttggccatt ctgggatact
     1681 ggttgctcat tgcaggcgta actttacgca ttctaatgaa acgacgcgca gttccctccg
     1741 cgatggcctg gctgttgatt atttacattc tgccgttagt cggaattatt gcctatcttg
     1801 ccgttggcga gctccattta ggcaaacgcc gcgctgagcg cgccagagcg atgtggcctt
     1861 ccaccgcaaa atggcttaac gaccttaaag cctgtaagca tatcttcgcc gaagaaaata
     1921 gcagtgtcgc tgcgccatta ttcaagcttt gcgagcgtcg tcaggggatc gctggggtca
     1981 aagggaatca gctacaactg atgaccgagt cagatgatgt gatgcaggcg ttaatccgcg
     2041 acatccagct cgcgcgccat aatattgaga tggtgtttta tatctggcag cccggcggca
     2101 tggcggacca ggtggctgaa tcattaatgg cggctgcacg gcgtggtatt cattgccgat
     2161 tgatgctcga ctccgccggg agtgtggctt ttttccgcag cccgtggccc gagctaatgc
     2221 gtaatgccgg tattgaagtg gtcgaagcct taaaggtcaa tctgatgcgt gtgtttttac
     2281 gccgtatgga cctgcgccaa catcgcaaga tgatcatgat cgataattac atcgcgtaca
     2341 ccggcagcat gaatatggtc gatcctcgct acttcaaaca agatgcgggc gtagggcaat
     2401 ggattgatct gatggcgcgt atggaaggcc ccatcgccac cgcgatgggg attatttatt
     2461 cctgcgactg ggagattgaa accggaaaac gtattctgcc gccaccacca gatgtcaata
     2521 ttatgccgtt tgaacaggcc agtggtcaca ccattcacac aattgcttct ggccccggct
     2581 ttccggaaga tctcattcac caggcattat tgactgcggc ttattcggcg catgaatatt
     2641 tgatcatgac cacgccctac tttgtgccaa gcgatgattt acttcatgcg atttgcacgg
     2701 cggcgcagcg cggggtggat gtcagtatta tccttccgcg aaaaaatgac tcgatgctgg
     2761 tcggctgggc cagtcgcgca ttctttacgg aactgcttgc tgctggggtt aaaatttatc
     2821 agtttgaagg cgggttactg cataccaaga gcgtgctggt cgatggcgaa ctaagtctgg
     2881 ttggcaccgt taaccttgat atgcgtagtc tgtggctgaa tttcgagatt accctggcaa
     2941 tcgacgataa aggttttggt gcagaccttg ccgccgttca ggacgattat atttcgcgtt
     3001 cacgtctgct cgatgcccgt ttatggctaa aacgtccatt atggcaacgt gtcgccgagc
     3061 gactgtttta cttcttcagc ccgttgctgt aaaacgtcgc caacagaggt taaacaggta
     3121 gtgactatgg atatggatct aaacaatcgc ctgactgaag atgaaacgct taagcaggcc
     3181 tacgatattt ttctcgaact ggctgccgac aatctcgatc cagctgatgt tctgctgttc
     3241 aatcttcagt ttgaagagcg cggcggcgcg gagttattcg atccggcgga agactggcag
     3301 gaacatgttg atttcgattt gaacccggac ttttttgccg aagtggtgat tggtctggcg
     3361 gacagtgaag atggtgaaat caacgatgtt ttcgcccgca ttctgttatg ccgcgaaaaa
     3421 gatcacaaac tttgccatat catctggcgg gaataaataa tcgcttttag cgtgaaaaag
     3481 ggctgacaac tgtcagccct tattgttttt aaagcggatc gactttcagg caagaaacgg
     3541 cgtgtctgaa actcccctcc agaacaggac gtgttttggc gcactccgga ccggcaatcg
     3601 ggcaacgggt acggaaaaca caaccggaag gcgggttgat cggcgacggt aattcccctt
     3661 ccagtaactg gatggttttg ttcttctcca gatccggatc aggtatgggg actgccgaca
     3721 tcaatgccct ggtgtaagga tgtagtggat tgtggtagac ctcatcatag gtccccagtt
     3781 ctactgcatg gccgagatac atcaccaaca cacgatcgga aatgtgtttt accacggcca
     3841 ggtcatgagc aataaaaatt aatgacaatc ccatctcacg ttgcagctgc tggagcaggt
     3901 tgaccacctg cgcctgaatt gacacgtcca gcgccgaaac cggctcatcg cagataatca
     3961 gcttcggttc aagaataaga gcacgagcaa tcccgatacg ctggcactgc ccaccggaga
     4021 actcatgcgg atagcggtta atcaggttag gcaataaccc gactttcagc atcatcgcct
     4081 tcacgcgctc gcgaacttcc tggcgtgaca ttttcggatg ataagtacgc agtggttcag
     4141 cgatgatctc gccgatggtc atacgcgggt ttagcgatgc caacggatcc tggaaaatca
     4201 tctgaatatc actgcgaacg gcacgccatt catcgggctt catgcccagc aactctttac
     4261 ctaaccaggc aacatgaccg tcggtcgcct tgaccaaacc gatgatggcg cgagcaaagg
     4321 tggacttacc gcatcccgat tcccctacca cacctaatgt ttccccttca tacaggcgaa
     4381 gagtgacacc atcgacggct ttgagcgttt tcggcggttg ccagaaccac tgtttgccat
     4441 ctttgatttc aaagtgcact ttaagatcgg cgatttcaag gaggactttt cttccttcag
     4501 ttacagcatt cataacagtt cctccaccgg tttaaagcaa gcacgcagac ggccaggcgt
     4561 aaactcttcc agcggcggtg cgctactaca aatttccatc gcatgtggac aacgtggctg
     4621 gaacgggcaa cctttcggta atcgcagcaa gtttggcgga ttacccggga tggtcaacat
     4681 tgtttcacct tccgcatcaa gacgcggcac cgcgttgagc aaaccgatag aataaggatg
     4741 aacgggttga taaaagacat cgcgcgcatt gccatattcc atcgtgcgcc cggcgtacat
     4801 taccagcact ttgtcacaga tccccgccac cacgccaaga tcgtgggtga tcataatgat
     4861 ggcagtatta aattcccgct tcagttcatt caatagcgtc atgatctgcg cctgtacggt
     4921 gacgtccagc gctgtagtgg gttcatccgc aatcagcaac ttaggtcgac atagcaatgc
     4981 catcgcaatc atgactcgct gacgcatgcc gccagaaaat tcgtgcgggt acattttcat
     5041 gcgtttacgt gcttccggca tttttaccgc atcgagcatc cgcaccgact cttcaaacgc
     5101 ctcagctttg ctcatgttct tatgcagcat cagcacttcc atcaactgct caccgacgcg
     5161 catataggga ttcaacgaag tcattgggtc ctgaaaaatc attgagattt gttcagcgcg
     5221 cagtttattg agttcacgct ctggcaaatt gaggatttca cgcccattga aggttgccgc
     5281 tccgccaatg cgtccgttgg cagccaacag gcccatcaac gcaaatgcag tttgcgattt
     5341 acccgaacca gactcaccta caatgcccag cgtttctccg gcacgtaggg aaaaatttaa
     5401 atcattgacc gccgtgacgt cgccgtccgg ggtactaaag gtgacacgca aatctttcac
     5461 gttcagcagt gcgtcagcct gttgttgtgc gagcggcaca gttgcagttt caattacgct
     5521 catggctgca ctccttaacg atctttcggg tcgagggcat cacgcaagcc atcgccgata
     5581 aagttgaaac aaaacagtgt caccacaagg aatcccgctg ggaacaacag taaccatgga
     5641 gagacttcca tcgagttcgc gccatcactc agcaatgccc cccagctgct taacggctct
     5701 tgcgtaccca accccaggaa gctaaggaaa gattcaaaga ggatcatgct gggcaccagt
     5761 agcgatgcgt agaccaccac cacaccgagt acgttcggca caatgtggcg aataacaatg
     5821 cccggcgtcg atacaccgcc aacttgtgcc gcctcaataa actctttgcg cttcagactc
     5881 agggtttgcc cacgcacaat acgagccata tccagccagg aaaccatgcc aatcgccacg
     5941 aaaatcagca ggatgttttg gccgaaaaag gtcaccagca aaatgacgaa gaacatgaat
     6001 gggaaggagt tgaggatttc cagcagacgc atcattaccg aatccacttt accgcccaga
     6061 taaccggaaa gcgaaccata aagtgtcccc acgaccactg ccaccagtgc cgcagcaaca
     6121 ccgaccatga gtgagatacg cccgccaatc gcaacgcgca caagcaggtc gcgaccggat
     6181 gagtcagtac caaagtagtg accggactcc atatccgggg cgctggacat catcgcccag
     6241 tcagtatcgt cataggcaaa ctgggaaagc atcggtgcca ggattacaaa taacgcgatc
     6301 agcaccagca ctatcagact ggcaaccgcc gcacggttat gcataaaacg tcgacgtgcg
     6361 tcctgccaca agctgcgccc ttcgacctcc agcttttcac tgaaattttc cagcgtctcg
     6421 ctgtttttct tacttaacat catcgcgagc tccagtatca gtaacggatt ttcgggtcga
     6481 taaccgcata tagcacatcg acaatggcat taaacaaaat ggttaacgca ccaaccagga
     6541 tggtcaggct taacactaag gaatagtcac ggttcaatgc gccattaacg aacagttgcc
     6601 caatccccgg caaaccataa atggtttcga tgaccataga accggtaata atgccgacaa
     6661 atgcggggcc catataggag agcacgggta acagagcagg ttttaatgcg tggcgtaaaa
     6721 tgatccgccg cataggtaac cctttcgccc gggcagtacg aataaagttg gagtgtaata
     6781 cttcaatcat agagccacgg gtaatacgcg caatactggc gatataggcg agtgacaacg
     6841 ccaccatcgg caatatcatg aatttaagcg ccccaccatt ccagccaccg cccggcagcc
     6901 aatgcaaaat gatcgcaaat atcatgacta ataatggcgc aaccacaaaa ctggggataa
     6961 caaccccggt cattgccagc cccatcacgg tatagtccca tttggtgttt tgttttaatg
     7021 cggcaataac gccagcacta acacccagta ttaccgcaag gaaaaatgcg gcggctccca
     7081 gtttggcaga aacgggaaaa ctggatgcca ccaggtcatt gaccgaataa tctttatatt
     7141 taaacgatgg accgaaatcg ccatgcgcca gttgtttcag gtagctgaaa tactgtgtca
     7201 tgatcggatc attaagatga tatttcgctt cgatattggc catcacttct ggcggtaaag
     7261 tacgttcgcc ggtaaaaggg cttcccggcg cgaggcgcat cataaagaac gaaatagtaa
     7321 taagaataaa tagcgtcgga atcgcttcca gacagcgacg tagaataaat tttaacattg
     7381 ccctaccttc tggcctgtgc cttctgtaat gcgataaaaa tcagacaccg tggagcagga
     7441 cactcctgcc ccacgtattg ccattagtgc ttcacaatgt acatattccg ggtataggta
     7501 ttatccagcg gatctttgcc ggtatagcca ccaacccacg gtttcaccag acgcgcattc
     7561 acgtagtaat aaacaggaac aatggccgaa tccttatcca gctgttgttc tgctttagtg
     7621 tacagagctg tgcgctgcgc ctcgtcagtc actttcagcg tttccgccat aatgctgtca
     7681 aaggccgggc tcttataatg cgcggtattc atcgagctgt tcgacagcat ggtgttcagg
     7741 aaggaagttg gttcgttgta gtctgcacac cagcctgcac gggccacatc aaaagtgccc
     7801 tggtgacggg tgtcgaggaa cgttttccac tcctggttaa ccagtttgac gtttacacca
     7861 atgtttttct tccacaatga agaggcagca atcgccagct ttttatgcag atcggaggtg
     7921 ttataccgca ggttgatggt caacggtttg tctgcggtat aacccgcttc agccagcagt
     7981 tttttcgctt cttcgttacg tttttcctgg ctccagccaa accattctgg ctgagtcaat
     8041 tttgcgccat cagtatacgg tggagtgtaa ccataggcgg gcatgtcgcc ctgcgctttc
     8101 actttattaa caatgatatc gcggtccata cctagtttca gtgcggtacg cacacgcaca
     8161 tcgttgaatg gcggtttctg gttgttaatt tcgtaatagt aagtgcacag gtatggatca
     8221 acgtgaactt cgtccgggat ctcttttttc agcttctgga acaattcgat cggcatgttg
     8281 ttatacgtca tgtcgatttc accactacgg tagcggttga catcggtaac ttcagaagca
     8341 ataggcaaat aggttacctg gttaataacg gttttcgcgt tgttccagta ggtcgggctg
     8401 cgttcaagaa cgattcgttc gtttacgacc caatctttta aggtataggc accgttggtg
     8461 acgatattac caggctgggt ccatttttcg ccgaatttct cgatagcggc ttttggcacc
     8521 ggtgaagttg atgggtgaac aagtaattta tagaagtacg gaacgggttc acttaaggtg
     8581 acttctaatg tgtgatcatc aatagctttc acgccgagat cggtaatcgg ttttttccct
     8641 tcaagaattt catcaatacc ggcgatatgc ccatattgca gataactggc atacggagaa
     8701 gcagtgttcg gatcaacaga acgttgccag ctatacacaa agtcttgtgc tgtgactggc
     8761 gtgccatcag accatttcgc atctttacgc aaatggaagg tccagacttt cgcgtcttta
     8821 ttatcccagg attcagcgac gccaggtgct ggatgaccgt caagatcgct gaccagtaag
     8881 ccttcaaaca ggtctcggct gatattagac tccggaacac cttcaatttt gtgcggatct
     8941 aatgactgaa cttctgaacc attgttacgt accagtgttt gtttttccgc cagtgtgacg
     9001 cctgcgggta catcagctgc cagcgcgaca ttccctgcca ttagcgcagc cagaacgcca
     9061 gctgctacta aacttctctt ggtgatgttg gtcattgttt tttggactcc ctcattataa
     9121 ttactggtat taaccagcat gtgtaatccc ggattgggac cctgcacagg ctcggagact
     9181 ttctgctgtc aggttacttt atcgttacgt attactatca ccgactttat ttattctggt
     9241 gattcagacg atcaccttat gtcgattcgt actgtgtctc ccctgtcgag acacattatt
     9301 ctttcgcgac gctctttaat tgttaattat tctcattacc aataaagaat cgtctggcgg
     9361 ttgcccggaa agtaccaaat gcggttctta ctcgccaata cattttgcaa aattgttaag
     9421 cgattctctt ttattgaccg tatcagaatc tatagcttgc atccatgcct taacaaacat
     9481 ctttcaattc aatcaaatag agattggcta agcatgtcta tcttttctga ttacagttcc
     9541 agtagtgaaa tgcacaataa tttaacaatt gattattatt tagcactttc ctctaccaaa
     9601 ggtagtggaa ttactaatat catttcaatt attcttcagc aagcccagga ttatgatgtg
     9661 gcgaaaataa catgatggtg aggagaagca gttaaggaaa tccttataac taactgtaat
     9721 ttgagaagaa tatttttacc tattcctttt gcttttgata actgcgcggg aacaaagtaa
     9781 aaataatgac gaagtaaacg gagcatggca gctccgtttc atttgaaagg aattaattaa
     9841 gcaggccggg gaaaatcccc ttaataccag taacgataaa ttcaatcccc aatgccatca
     9901 gcaatagccc cataatacgc gtaatcacgt tgatgccggt ctggcgtaaa acccgtacca
     9961 gccacggtgc catgcggaac aatccccagc aacataaagc gaacaatgca atagccacaa
    10021 agaaaccaaa cagatagcta atgctgtgat aacgcgtacc ccagacgatg gtagaactga
    10081 tcgcccctgg ccccgccatc aacggcaacg ccaatggcac cacaccaata ctttcacgta
    10141 ccgcggtttc tgatttttct tgcttgttct gtttatcctc gccaagcttg ccgctgatca
    10201 tcgacatcgc tatcgtcacc accaggatgc ccccggcgat acggaacgaa tcaattgata
    10261 taccaaaaag ttgtagaatc gtgtcgccga gaaaaagcga gatccacaag ataatggcca
    10321 cagacaggtt ggctgtaagg ttagttttgt ttcgcgctgc cgctgtctga taactggtca
    10381 tgctgataaa gacgggaata atccctaccg ggttgaccag cgcaaataac ccgatgaaaa
    10441 atttgaagta aacgggaaaa tcaaaaaggg tctgaatcac ggttagctcc gaagcaaaag
    10501 ccggataatg ttagccataa ataaggttga aaagacgcgc tgacaatacg ccttttgaca
    10561 gcatttttca cctcctaact acttaaaatt gctatcattc gttattgtta tctagttgtg
    10621 caaaacatgc taatgtagcc accaaatcat actacaattt attaactgtt agctataatg
    10681 gcgaaaagcg atgctgaaag gtgtcagctt tgcaaaaatt tgatttggat cacgtaatca
    10741 gtacccagaa gtgagtaatc ttgcttacgc cacctggaag tgacgcatta gagataataa
    10801 ctctaatgtt taaactcttt tagtaaatca cagtgagtgt gagcgcgaat aagcttttga
    10861 ttttcatcgg ttaagcaaat catcaccgca ctgactatac tctcgtattc gagcagatga
    10921 tttactaaaa aagtttaaca ttatcaggag agcattatgg ctgttactaa tgtcgctgaa
    10981 cttaacgcac tcgtagagcg tgtaaaaaaa gcccagcgtg aatatgccag tttcactcaa
    11041 gagcaagtag acaaaatctt ccgcgccgcc gctctggctg ctgcagatgc tcgaatccca
    11101 ctcgcgaaaa tggccgttgc cgaatccggc atgggtatcg tcgaagataa agtgatcaaa
    11161 aaccactttg cttctgaata tatctacaac gcctataaag atgaaaaaac ctgtggtgtt
    11221 ctgtctgaag acgacacttt tggtaccatc actatcgctg aaccaatcgg tattatttgc
    11281 ggtatcgttc cgaccactaa cccgacttca actgctatct tcaaatcgct gatcagcctg
    11341 aagacccgta acgccattat cttctccccg cacccgcgtg caaaagatgc aaccaacaaa
    11401 gcggctgata tcgttctgca ggctgctatc gctgccggtg ctccgaaaga tctgatcggc
    11461 tggatcgatc aaccttctgt tgaactgtct aacgcactga tgcaccaccc agacatcaac
    11521 ctgatcctcg cgactggtgg tccgggcatg gttaaagccg catacagctc cggtaaacca
    11581 gctatcggtg taggcgcggg taacactcca gttgttattg atgaaactgc tgatatcaaa
    11641 cgtgcagttg catctgtact gatgtccaaa accttcgaca acggcgtaat ctgtgcttct
    11701 gaacagtctg ttgttgttgt tgactctgtt tatgacgcag tacgtgaacg tttcgcaacc
    11761 cacggcggct atctgttgca gggtaaagag ctgaaagctg ttcaggatgt tatcctgaaa
    11821 aatggtgcgc tgaacgcggc tatcgttggt cagccagcct ataaaattgc tgaactggca
    11881 ggcttctctg taccagaaaa caccaagatt ctgatcggtg aagtgaccgt tgttgatgaa
    11941 agcgaaccgt tcgcacatga aaaactgtcc ccgactctgg caatgtaccg cgctaaagat
    12001 ttcgaagacg cggtagaaaa agcagagaaa ctggttgcta tgggcggtat cggtcatacc
    12061 tcttgcctgt acactgacca ggataaccaa ccggctcgcg tttcttactt cggtcagaaa
    12121 atgaaaacag ctcgtatcct gattaacacc ccggcttctc agggtggtat cggtgacctg
    12181 tataacttca aactcgcacc ttccctgact ctgggttgtg gttcctgggg tggtaactcc
    12241 atctctgaaa acgttggtcc gaaacacctg atcaacaaga aaaccgttgc taagcgagct
    12301 gaaaacatgt tgtggcacaa acttccgaaa tctatctact tccgccgtgg ctccctgcca
    12361 atcgcgctgg atgaagtgat tactgatggc cacaaacgtg cgctcatcgt gactgaccgc
    12421 ttcctgttca acaatggtta tgctgatcag atcacttccg tactgaaagc agcaggcgtt
    12481 gaaactgaag tcttcttcga agtagaagcg gacccgaccc tgagcatcgt tcgtaaaggt
    12541 gcagaactgg caaactcctt caaaccagac gtgattatcg cgctgggtgg tggttccccg
    12601 atggacgccg cgaagatcat gtgggttatg tacgaacatc cggaaactca cttcgaagag
    12661 ctggcgctgc gctttatgga tatccgtaaa cgtatctaca agttcccgaa aatgggcgtg
    12721 aaagcgaaaa tgatcgctgt caccaccact tctggtacag gttctgaagt cactccgttt
    12781 gcggttgtaa ctgacgacgc tactggtcag aaatacccgc tggcggacta tgcgctgacc
    12841 ccggatatgg cgattgtcga cgccaacctg gttatggaca tgccgaagtc cctgtgtgct
    12901 ttcggtggtc tggacgcagt aactcacgcc atggaagctt atgtttctgt actggcatct
    12961 gagttctctg atggtcaggc tctgcaggca ctgaaactgc tgaaagaata tctgccagcg
    13021 tcctaccacg aagggtctaa aaatccggta gcgcgtgaac gtgttcacag tgcagcaact
    13081 atcgcgggta tcgcatttgc gaacgccttc ctgggggtat gtcactcaat ggcgcacaaa
    13141 ctgggttccc agttccatat tccgcacggt ctggcaaacg ccctgctgat ttgtaacgtt
    13201 attcgctaca atgcgaacga caacccgacc aagcagactg cattcagcca gtatgaccgt
    13261 ccgcaggctc gccgtcgtta tgctgaaatt gccgaccacc tgggtctgag cgcaccgggc
    13321 gaccgtactg ctgctaagat cgagaaactg ctggcatggc tggaaacgct gaaagctgaa
    13381 ctgggtattc cgaaatctat ccgtgaagct ggcgttcagg aagcagactt cctggcgaac
    13441 gtggataaac tgtctgaaga tgcattcgat gaccagtgca ccggcgctaa cccgcgttac
    13501 ccgctgatct ccgagctgaa acagattctg ctggatacct actacggtcg tgattatgta
    13561 gaaggtgaaa ctgcagcgaa aaaagaagcc gctccggcta aagctgagaa aaaagcgaaa
    13621 aaatccgctt aatcagtagc gctgtctggc aatataaacg gccccttc
//
LOCUS       DAJTNM010000106        13428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTNM010000106 DAJTNM010000000
VERSION     DAJTNM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.106"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..353
                     /locus_tag="OO172_003139"
     CDS             <1..353
                     /locus_tag="OO172_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513231.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
     gene            510..1259
                     /locus_tag="OO172_003140"
     CDS             510..1259
                     /locus_tag="OO172_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5513232.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1281..1847
                     /gene="yieF"
                     /locus_tag="OO172_003141"
                     /gene_synonym="chrR"
     CDS             1281..1847
                     /gene="yieF"
                     /locus_tag="OO172_003141"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5513233.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1901..3238)
                     /gene="adeP"
                     /locus_tag="OO172_003142"
     CDS             complement(1901..3238)
                     /gene="adeP"
                     /locus_tag="OO172_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5513234.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3403..4068
                     /gene="yieH"
                     /locus_tag="OO172_003143"
     CDS             3403..4068
                     /gene="yieH"
                     /locus_tag="OO172_003143"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5513235.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4135..4608
                     /locus_tag="OO172_003144"
     CDS             4135..4608
                     /locus_tag="OO172_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR5513236.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4657..5244
                     /locus_tag="OO172_003145"
     CDS             4657..5244
                     /locus_tag="OO172_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR5513237.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5306..6028)
                     /locus_tag="OO172_003146"
     CDS             complement(5306..6028)
                     /locus_tag="OO172_003146"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5513238.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPILGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNMLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6030..7205)
                     /locus_tag="OO172_003147"
     CDS             complement(6030..7205)
                     /locus_tag="OO172_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR5513239.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYLLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7232..8848)
                     /gene="bglH"
                     /locus_tag="OO172_003148"
     CDS             complement(7232..8848)
                     /gene="bglH"
                     /locus_tag="OO172_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR5513240.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8934..10331)
                     /gene="bglB"
                     /locus_tag="OO172_003149"
     CDS             complement(8934..10331)
                     /gene="bglB"
                     /locus_tag="OO172_003149"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5513241.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10347..12224)
                     /gene="bglF"
                     /locus_tag="OO172_003150"
     CDS             complement(10347..12224)
                     /gene="bglF"
                     /locus_tag="OO172_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5513242.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(12358..13194)
                     /gene="bglG"
                     /locus_tag="OO172_003151"
     CDS             complement(12358..13194)
                     /gene="bglG"
                     /locus_tag="OO172_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5513243.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         3543 a         3373 c         3079 g         3433 t
ORIGIN      
        1 atttcaggaa ggagtgtccg ttaaactcag gctacctcag ggcgctggac aatgtgttac
       61 aggagctggg gcgcgaacgc acgattgcta tgagcctgcc ggaattcgag cagtcactgt
      121 ttatggcagc gcaacccgac aatctgctac tggcgaccgc gccgcgctac tgtcagtact
      181 acaatcaact ccatcaactg ccgttggttg ctcttcctct cccgtttgac gaaagccagc
      241 aaaaaaagct ggaagttcct tttaccctgc tgtggcataa acggaacagc cataatccga
      301 agatcgtctg gttacgggaa accattaaaa acctttacgc gtcgatggca taaccgaatc
      361 gtatgaaacg cgacccaatt tcacaataaa atgtaaaaaa gttgtaataa gcttgtctga
      421 atcgaacttt tagccgcttt agtctgtcca tcattccagt aaatgattac tcttgtattc
      481 ataatggacc attaagcatg gagcgaaaaa tggcgactca ctttgcccga gggattttaa
      541 cggaaggaca tctgatttct gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca
      601 ttccccctca tcgtcaaagc cgttttctgg cgtccagagg tttactcgca gaactgatgt
      661 tcatgctgta tggcattggc gaattgccgg aaatcgtcac cctgccgaaa ggtaaaccgg
      721 ttttcagtga taaaaatttg ccttcgtttt ctatttccta tgccgggaat atggttggcg
      781 tggcgttaac aaccgaaggt gaatgtggcc tcgatatgga actacagcgt gcgacgcgcg
      841 ggtttcatag cccacacgcg cccgataacc acaccttttc cagcaatgaa tcgctatgga
      901 tcagtaaaca aaacgatcct aacgaagcgc gggcgcagct cattacgctg cgccgaagcg
      961 tgctaaaact aaccggtgat gttttgaatg acgatccgcg cgatctgcag ctgctgccca
     1021 ttgccggacg cctgaaatgt gctcatgtaa atcatgtaga agcgttatgc gacgcggagg
     1081 acgtgctggt gtggtccgtg gcggtcacgc ccgcgattga aaagctcagt gtctgggagt
     1141 tagatggcaa acacagctgg aaaagcctgc cggatattca cagccgcgcc aacaatccta
     1201 ccagccggat gatgcgtttt gcccagctct ctaccgtgaa gtctttttcg ccaaattgat
     1261 agacaaccac aggagtcatt atgtctgaaa aattgcaggt ggttacgtta ctggggagcc
     1321 tgcgcaaagg ctcatttaat ggcatggttg cacgtaccct gccgaaaatt gctccggcga
     1381 gcatggaagt caatgcgtta ccttccattg ccgacattcc cctgtatgac gctgacgtac
     1441 agcaggaaga aggttttcca gcaacggttg aagctctggc ggaacagatc cgtcaggctg
     1501 acggtgtggt gatcgtcacg ccggaatata actactcggt accgggtggg ctgaaaaatg
     1561 ccatcgactg gctttcccgc ctgccggatc aaccgctggc cggtaaaccg gtgttgattc
     1621 agaccagctc aatgggcgtg attggcggcg cgcgctgtca gtatcacctg cgccagattc
     1681 tggttttcct cgatgcaatg gtgatgaaca agccggaatt tatgggcggc gtgattcaga
     1741 acaaagttga tccgcaaacc ggagaagtga ttgatcaggg tacgctggac cacctgaccg
     1801 ggcaattgac cgcatttggt gagtttattc agcgagttaa gatctaaata aaaaacccgc
     1861 cagtaatcat gcatggcggg tttttaatgc gctatcgatt ttaatgagcg tcgataaata
     1921 caatcttcag gataaacagc agcgcaacga tgattacgca cgggctaagg tcacgcagac
     1981 gcccggtacc aatcttcatc acgcagtagg agataaagcc cagcgcaata ccttcggtaa
     2041 tcgaaaagct gaacggcatc atcacggcgg taataaacgc cggaacagat tcagtaagat
     2101 cctgccagtt cacgcgagca agacttgagg tcatcaacac gccaacgtag atcaacgcgc
     2161 cagctgcagc gtagcctggc accattcccg ccagcggcga cagaaagata accagcagga
     2221 acagcagacc aacaaccact gccgtcagac cggtacgacc gccaaccgat acgccggaag
     2281 aggactcaat ataagccgta acggaagaag taccgataaa cgaaccggtc acggaagaga
     2341 tactgtcgac atacagcgcc tgcttcatgc gcgggaattt ccccttctca tccgccagac
     2401 ctgctttatc ggtcacgcca atcagcgtac cggaggagtc aaacaagttg accaacatga
     2461 aagagaaaat cacccctgcc agcccgaggt taaacgaccc ggctaaatct acatgaccca
     2521 caactgtcat tacgctcggc ggcgcagaaa cgatgccatt gtagtgcaca tcacccagca
     2581 tccagcccag cagcgtcgtc accacgatag aaaccagcac cgctgcgtga atgttgcgcg
     2641 aggccagaat agcaatgatg aaaaagccga ggatacccag aagtacgctg tgagaagtca
     2701 gattaccgat gctcaccagc gtttccgagt tagcgacaat cacacctgcg tttttcagcc
     2761 ccatcatgcc gataaacagg ccgataccgc tggtaatacc cacacgcaga cttaccggaa
     2821 tgttagcaat catccagtaa cgaacgcgga aaatggtcaa aagaagcagt ccgacagcgc
     2881 cccagaagat tgcgcccata ccgatctgcc acggcagccc cattgcctgg acaaccacaa
     2941 aagcgaaaaa ggcgttaagc cccatcgcag gagccagcgc caccggaagg tttgcaaaca
     3001 atcccatcat aatgctaccg aaagcagcaa tcagacaggt tgttacgaag acggcactgg
     3061 tgtccatgcc agcaacgccc agaatttgtg ggttaacaaa tacgatgtag accatcgtca
     3121 ggaaggtggt gaacccggca atcacttcag ttcgtgccgt agtaccatgt tcgcgcagtt
     3181 taaaaacgcg ttccagcatc ctctgaccag aagtctgggt ggtatgttga tgactcatta
     3241 tctatttccg aacaaggagg gaaaatccgt cgctatcgta taccaaaatg cgacaatagg
     3301 cgcgtttgtg agagactttt ttattggatt tacttatacg gcaacgattg cgttgcgcaa
     3361 atcggctcta cgaaaacgtt aaactgatta aaaaggaaag gcatgtcccg gatagaagcg
     3421 gtatttttcg actgcgacgg tacgctggtc gacagtgaag tcatttgctc ccgcgcatat
     3481 gtaacgatgt ttcaggaatt tggtattacg ctcgatcctg aagaggtatt caaacgtttc
     3541 aaaggtgtaa aactgtacga aattatcgat attgtttccc ttgaacatgg tgttacgtta
     3601 gcgaaaacag aagctgaaca cgtttaccgt gcagaagtcg ctcggctgtt cgattcagaa
     3661 ctggaagcca tcgaaggggc tggagcgctc ctgtcagcga tcactgcgcc aatgtgtgtg
     3721 gtatctaacg gcccaaataa caaaatgcag cattctatgg gcaagctgaa tatgttgcac
     3781 tacttcccgg ataaactgtt cagcggctac gatattcagc gctggaagcc agacccggcg
     3841 ttaatgttcc atgcggcaaa agcgatgaat gtaaatgtag aaaactgcat tctggttgat
     3901 gactcagttg ccggtgcgca atctggtatc gacgcaggta tggaagtgtt ctacttctgc
     3961 gccgacccgc acaataagcc gatcgttcac ccgaaagtca ccacctttac ccatctttcg
     4021 cagttaccgg aactgtggaa agcgcgtggt tgggatatta cggcatagtt cttcacactc
     4081 ccttcactta ccccgcttaa attggcgctc aaaggtaagt aaagggagtt tgatatgtct
     4141 gtttcacgtc gggtaataca tcacggactt tattttgcgg ttttaggacc gttaattggt
     4201 gttctgtttc ttgtcctcta catattcttc gcaaaagaac cgctggttct tttggtaata
     4261 atacaggtac ttccattgtt tctcttattg tcgattacta cgggagctat tcctgcattg
     4321 ttaaccggcg tgatggtcgc ctgtctgccg gaaaagatcg ggtcacagaa aaattatcgt
     4381 tgtctggctg gtggcatagg tggcgtcgtt atcaccgaga tctattgtgc agttattgta
     4441 catattaagg gcatggcttc ctcggagttg tttgaaaaca ttctttctgg tgacagtctc
     4501 gttgtccgca tcattcctgc attgctggca ggtgtggtga tgagcagaat cattacccgt
     4561 ctgcccggat tggatatttc atgtcctgaa acagactctt taagttaagc gggatacttt
     4621 atctttgggc tactcaaaag cagacaggat gttactatga ctcaaaatat caggccgtta
     4681 ccccaattca aatatcatcc caagccactg gaaacaggcg catttgaaca ggataaaacc
     4741 gtagagtgcg attgctgtga acaacagacg tcagtttatt actcgggtcc cttttattgc
     4801 gttgatgaag ttgaacatct ctgtccgttg tgtattgcgg acggttctgc tgctgaaaag
     4861 tttgcaggta gttttcagga tgatgccagc atagaaggtg ttgaatttga gtatgatgaa
     4921 gaggacgaat ttgccggtat taagaacaca tatcctgatg aaatgctgaa agagttggtt
     4981 gaacgcacgc caggttatca tggatggcaa caggaattct ggcttgcgca ttgtggcgat
     5041 ttctgtgctt ttatcggcta tgtgggctgg aatgatataa aagatcgcct cgatgaattt
     5101 gccaaccttg aagaagattg tgagaatttc ggtattagaa attctgatct agctaaatgc
     5161 ctgcaaaagg gtggtgattg tcagggttat ctcttccgct gtctccactg cggcaagctg
     5221 agactgtggg gtgatttttc gtagttattt aaataatgag aacaggccgg agcgtaattc
     5281 atacatccgg ccttatttct tcagcttaac cgaacgctaa ttctgcggca gcggctttat
     5341 cagcgattac catcagcgaa ggatgcagct gcaacacgga agcaggaaca tcttcggtaa
     5401 ccggaccttg cagcatattt ttcagtgcct gcgctttacc tgcgccactg acaattatca
     5461 gcaagttttt cgccgccatg atgcttttcg gccccatcgt cacgtagctg tcaggcacta
     5521 acgaaaagtc gccgcccaac tcaccatgcg ccacaatatc aaccatctct cccaggatcg
     5581 ggaactccac ggtttgctca tggaaatggg tcgtattcgg caaattgcca caaaaatgac
     5641 catctgcacc taatcccagc accaccaaat ccaggccgcc ttcacgcgcc agtttctgat
     5701 catgctcgcg gtagttatca atagtgagct tctggatatt ctcttctttg atccccgcag
     5761 gggtgaaaaa cagattacgc agattggtaa tcgttacgcc ttctccctct ttgccgcgaa
     5821 agggaatttc atcgaaatta tagaaatagc agttatcgta ccaaggctta cctttaacca
     5881 gggtggtgag gtattcatac atgcctttgg gcgtgctacc ggcggtaatt gccaggttaa
     5941 cacgacgcgt cttcgacata taaccaagca gatggtgtgc cgcgacacgg ctcatttcct
     6001 ggtaatcttc ggtaatgatt aatttcatct taatgcctta ttttaaattt ctgtacgaag
     6061 gctgcatagg caggccgcca gacatccatt tcatggttca ggccgggata ttcctggtaa
     6121 tcaaacttaa tttttttctg ctcaagctca gttttcagcc cggcgatatc cttgccggtt
     6181 acgacatctt tatcaccaac gaccacagta aaattacgta gttgctggtt aatagctgcc
     6241 ggatcgttca gccgggccgc gacaccttca tccggtacgg ttgtcgtggt aacaccactg
     6301 aatgtggcca gccagccaaa gctttccaga tgattcattc cggaaaccag cgcctggtac
     6361 ccgccttgtg aaagccctgc cagcgcgcga ccatcggcat ctttacggac actaaaacgc
     6421 ttgctaatca gcgggataat atcgttcatc agttcgcgat ctgccgcttt agcattcagc
     6481 agataaaaga ctttacgtct ttcctgaggc acgaaatctt cgggaataat gcccttcgca
     6541 tcggtttcgg tatccgggat caccaccagc atcggtttaa ttttcccttc agccagcagg
     6601 ttatccatga tttgcgggat acgcccctga tcgatagcgg aacgtccagt atcaccaaaa
     6661 ccgtgataga aatagagcac tggcaaaggc tcgcccatgc cggtgtatcc tggcggggtc
     6721 cagacataca tctgacgttc agattgcaat gcgttggagt ggtaagttat ggcgatcaaa
     6781 tcaccatgcg caacagaacg cgtatccaga taactgccta gcaccagaat catactggag
     6841 ttaacctggc gctgaggctt agtcattgcg gtgcctgtat caatgctgcg tacaccatca
     6901 acattgaaaa aatactcgta caggttgcct ttcaggatag gtgtgcgcca cgaccagact
     6961 cctgcttcgt ctttggtcat cgggtgaata ttgtccggaa ccggaacacc caccactaca
     7021 gagacatttt ttgccccagg ggcaaagtag cgaaaggtca cgctgttatc ggcattgacc
     7081 tgagtcacat actgtttcac cgggatagtg ggtgccgggc tggctggcat atccgctgct
     7141 atggcccact gagcagaaat accgctggca atagccagcg ttaaagcagc aatttttata
     7201 ttcatcgtaa tattcctttc attaagttcc attaccacca gatttcagcc tgggcaccga
     7261 cagcaaactg gtcgtcttta ttgtcttcga aggtgaagcc atccagttcg ttttccaggg
     7321 ctttgatata cgtggcgtag aaacgaattt ccagacgcga ggtcaacata ctggtattga
     7381 ctttaaaggt atggaagagc gtggttttat aaccagactc attaaattta ttactattcg
     7441 catctttgtt ttgctgggtg aaatagccca gttcaacacc tgtctggtta tattggtccc
     7501 aaatataggc cgggcgaaca accgcacgaa tagattcgaa atcagagtgt gcgcctgttt
     7561 cgtagctata aatatcgtta ccgaaggagt aaacaatcgc gttagccaca ataaaatgat
     7621 cgccgatata ggcttcgccc tgcgaggtca gacgaactgc tgttccgccg gtgtgatcac
     7681 cataataacg accattaaag gtggtaaatg gacttgcgcc agcataacgg ccaaagttcc
     7741 tggcgataga gttattcgca accaggaagg agaattcgtt gaagccacct ttatcaaatt
     7801 tctgcgttaa agatgtgccg aacatccagg tatctttcca gtcataatac ccgttattat
     7861 cctgattatc tttttccgat gcgctttcgt ttgccgtgac ataacgacca ctcaccatta
     7921 aggtcgcttt atcccataac gggatatctt tatagcgtaa atcaatagta tgggtattaa
     7981 tctgctgttt gttttgcagg ctgcgatcgt aatcatcgac atcttcgcga accagcgcga
     8041 tatcaatttt acccggaccg actttccagt tttccagacc tacacccgct gcggcatcag
     8101 tacgctgcgt tttccagtcc agcatctgga tttcaatttt cggcgcaccg tgtttaccca
     8161 cccagaaatc agcctctggc gcaaagggca ggaaaccttt ggtggtaacg tacatatcgg
     8221 agaactgcat aaagttctcg ccaccggcgt tatcgccaaa ccagccggta gagtactgct
     8281 gaccaacgtt accatccatc atcacaacgg catcaacccg tttgccgttt tcgttgtaga
     8341 cacgttgttt taactgcaaa tcaaaccagc cggagtattc gttaccaaag cggcccagag
     8401 aaccaatcgc ccatgattta ggtgaaccat gagaggcggt cccccaacca gaacggtaat
     8461 agccgttgta actaaagcca atttcatctt tcacaaactt actgaaatct ttcagagtca
     8521 tggaagaata aatcgagcct gtctcactgg catttttctc ttcatttttg accagtacag
     8581 catcaggttt tgcggcacta ctggtcggga acggattggc ggcataccct tgatcattcg
     8641 tacttacgct acgattcacc gtcgctggcg tatatttttt cttctcttca tctttatact
     8701 ttttgagttc gctttgcgtt tctcttaacg ctttttctaa taactcaagg cgttgttcca
     8761 ccgttaatga ttcagccaat gattgtgcag aaaaggctaa cggtgccatt aataagatgg
     8821 cagaggtaat aagatttcgt ctaaacataa tatcccttta tggtgcaaag aaagaattaa
     8881 cgtatcgcat ccagactgtt ctgaatgcga cgataattaa ggtgctttat tggttatttt
     8941 tttaatgaca gcccccgcgt tttgatcact tctgcatacc atccgaagct tttcttacgc
     9001 gttcttgtga ggcttccttc gccattatca tcacgatcca cataaataaa gccgtagcgc
     9061 ttagacattt gtgaatgaga tgcactgact aaatcaattg gcccccaact ggtgtacccc
     9121 ataatatcca caccatcggc aatcgcttca tttacctgta ccaggtgatc gtttaaatag
     9181 gcaattcgat aatcgtcctg tatcgaacca tccgcttcaa cgctgtcttt tacgcctaat
     9241 ccattctcga caataaataa cggtttttga taacgatccc aaagcgtatt taacagaacc
     9301 cgtaatccaa ccggatcaat ttgccacccc ctctctgaac ttttcagatg cggattgggg
     9361 atcatattca gtatgttgcc ctgcgcattt ttattaatgc tttcgtcgtg ggaaacacaa
     9421 ccagtcatgt aataactaaa agagatgaaa tctacagtat gttttaaatc ttctgcgtca
     9481 ctttcagtca tctcaatggt gatattgtgg tcgcggaaga aacgctgcat atagccggga
     9541 tactggccac gcgcctgaac atcaccaaag aacatccagc gccggttctc ttccatggcc
     9601 tgcaacatat cctgtggctg gcaggtgagg gggtaaacca gcccaccgag aagcatattg
     9661 ccgattttcg cttcggggat caggctatga taggctttaa ctgcccgcgc actggcaacc
     9721 agttgatgat ggatcgcctg ataaacttcc gcctcgccac tctcttctgc cagccccacg
     9781 cccgtgaatg gcgcgtgtaa cgacatattg atttcattaa acgtcagcca taacgctact
     9841 ttatgttggt agcgggtaaa gaccgtgcgg gcgtaatgct cgaagtgatc gatgaccgcc
     9901 cgattagccc aaccgccgta gtttttcacc agcccatata gcatttcgta atgggataac
     9961 gttaccagcg gcttgatccc cgcctgcgcc atttcatcaa acagccgatc gtaaaacgct
    10021 aaccccgctt cattcggttc ggcttcgtcg ccctgaggga aaattcgcgc ccaggcaatg
    10081 gaaatacgca gacaggtgaa gcccatctcg gcaaataacg cgatatcttc cgggtaacgg
    10141 tgataaaaat cgatggcgac atctttgata ttctctttcc ccaggatgcg cggttccatt
    10201 tttcccatta cgccatgagg ctgtaaatct gaggtcgtga tccctttgcc atcttccagc
    10261 caggcacctt ccacctgatt ggcagctgtt gcgccacccc aaagaaatgt ttctggaaat
    10321 gctttcgtaa ttaactcctt ttatcgttag cgaatgatgg ataacagcgg ttcacctgcg
    10381 cttatctgcg ccgtgccgtg gggtaatacg tctgtaaaat catcgctatt actgattaat
    10441 accggcgtcg tcagatcaaa tccggcctcg cgaatagcag ggatatcaaa agaaatcagc
    10501 cgatcgcctg tattgacctt gtcacccacg ttgacgtgag cggaaaagaa tttgccgtcc
    10561 agttttacgg tgtcgatacc gacatgaatc aggatctcca caccatcatc tgactcaatg
    10621 ccaatggcgt gtaatgtggc gaacaacgaa gcaattcgac ccgcaaccgg agaacgcact
    10681 tcaccaaccg agggcagaat ggcgatacct ttacccagca ggccactggc aaacgtggta
    10741 tcagcgacgt gaatgagcgg cacaatctct cccgtcatcg gtgaacagat accgccctgc
    10801 tcaggtggtg taataacctc tggtgttttc tcttgcgggg caccctgcgc tggctgacgt
    10861 ttactggtga tgaaatgaag catcaacgta ccaacaaatg cgcaacctat ggcaatgaca
    10921 ccgccaataa cgctggccca gacggtgaaa tcaatccccg ttgacgggat ggtctgcatg
    10981 aaggtgaaaa tacttggcaa accaaaggag taaactttcg tttgcgcgta gccaatgatg
    11041 gtggccccca atgctccact gatacaggcg ataacaaagg ggtacttacg cggcaggttg
    11101 acgccatata ccgctggttc ggtgatacca aacagacccg tcaacgccgc tgatcccgcc
    11161 accacttttt tctgcgcatc gcgttcgcag aggaagacgc cgagcgccgc cccgacctgc
    11221 gccataatgg cgggcattaa cagcgggatc atggtgtcgt agcccagcac ggtgaagtta
    11281 ttgatacaca gcggcaccag gccccagtgc agtccgaaca tgacgaagat ttgccagaag
    11341 ccgcccatta ccgcgcccgc aaatgcagga accgcctgat aaagccagag ataaccggcg
    11401 gcaatcattt cgcttatcca ggttgatagc ggccccacca gcagaaaggt gacgggtgtg
    11461 ataaccatca gacatagcaa tggtgtgaag aaatttttga ttgccgacgg taaccacgca
    11521 ttaagtcggc gttccagaat gctgcacaac caggcagaaa aaataatggg aataaccgat
    11581 gacgagtaat tcaacaatgt gaccggaata cccaggaaat ccagccccgg cgcatccgct
    11641 ttttgcccgt tctcgaaggc agtcagaatt aatggatgca ctaacgctcc accaatcacc
    11701 atggcagtaa atggattacc gccgaagcgt ttgcccgcgg tgtatcccag gattatcggg
    11761 aagaacccaa acaaggcatc actggcgctg aataaaatta aataagtacc actttgttcg
    11821 gtcgtccact gaaaagtgag cgccagagcc agcatacctt tcaggatccc ggttgccgcc
    11881 atcagaccga tcagaggcgt aaaaatacct gaaataacat aaacaaagcg gtttagcaga
    11941 ttacctttct catcattttc cggtgcctgt tgcgcttttt cgccaaggcc tgccacgctg
    12001 ttaaccgcca ggaagacatc ggccacatgg ttacctatga ccacctgaaa ctgaccaccg
    12061 ctttccacca ccataataat accgggggtc tttttcagta cctcagcttg cgctttgctt
    12121 tcatccttta acttaaaacg taatcgcgtt gcacaatgca tcagactcac aatgttatct
    12181 gcgcccccga ctcctgcgac tatttttctg gctaactccg tcataacttg ccctctaccg
    12241 ctttgcggca aaactccaaa aaaaaacctg aaaaaaacgg cctgacgtga atcaagcaat
    12301 ttttttcagg ttttgcccgc ttagtgcggt aacaatcctt tactcagtaa taatatttca
    12361 gtgttctttg cgcacgcgct ctatatttat ggctaaaaac ataatctctg cgggtgaaat
    12421 tttacgttga tactgcaaac caataaaaat ggcgatccgt tccgcacatt gccatgcttg
    12481 cgggtaattt tgttttactg cttgttgtaa tgattcatca ctatcgttaa ttgaagcatg
    12541 ttcaagaata cgccaggata aaaacttcag atgtgtaacc agtcgctgat aactcaagct
    12601 ttcttcctgg taattaaggc tgaactgaaa ttttattaat tgcagcattt cgcgcattaa
    12661 ctgcgtgaca cctgcaacat cctccatatt tccgctcatt tgggcactga ccagatgcat
    12721 ggcaataaag cccacttcat ctttcggtaa ctgcacgccc aaccgtttat caataatggt
    12781 taacgcttct tcccctagct ggaactcttt cgggtaaagc cgctggatat cccacagcaa
    12841 cgggttgggc agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt
    12901 tagcgagata taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag
    12961 acgatcacag gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc
    13021 gttcagttca tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc
    13081 gccagcgcgt ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc
    13141 atcaataacc accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag
    13201 gacctttttt ataaacaaaa aaacccgact tcaccagtat tctctggtta tgtcaggttt
    13261 tgcctgcgaa tgcagtaaca atccagtcat ttattaatgg tttttataac gaacatccag
    13321 gttcggaaat taatttaatt gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat
    13381 gaccatgctc gcagttatta actttgtgta attttaggaa tttataaa
//
LOCUS       DAJTNM010000107        13368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTNM010000107 DAJTNM010000000
VERSION     DAJTNM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.107"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(1..882)
                     /locus_tag="OO172_003152"
                     /pseudo
     CDS             complement(1..882)
                     /locus_tag="OO172_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1123..1923
                     /locus_tag="OO172_003153"
     CDS             1123..1923
                     /locus_tag="OO172_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5513244.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2001..2771
                     /locus_tag="OO172_003154"
     CDS             2001..2771
                     /locus_tag="OO172_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5513245.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2819..4177)
                     /gene="mltD"
                     /locus_tag="OO172_003155"
     CDS             complement(2819..4177)
                     /gene="mltD"
                     /locus_tag="OO172_003155"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5513246.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4249..5004)
                     /gene="gloB"
                     /locus_tag="OO172_003156"
     CDS             complement(4249..5004)
                     /gene="gloB"
                     /locus_tag="OO172_003156"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5513247.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5038..5760
                     /locus_tag="OO172_003157"
     CDS             5038..5760
                     /locus_tag="OO172_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5513248.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5757..6224)
                     /gene="rnhA"
                     /locus_tag="OO172_003158"
     CDS             complement(5757..6224)
                     /gene="rnhA"
                     /locus_tag="OO172_003158"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5513249.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6289..7020
                     /gene="dnaQ"
                     /locus_tag="OO172_003159"
     CDS             6289..7020
                     /gene="dnaQ"
                     /locus_tag="OO172_003159"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5513250.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7153..7229
                     /locus_tag="OO172_003160"
     tRNA            7153..7229
                     /locus_tag="OO172_003160"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7187..7189,aa:Asp,seq:gtc)
     gene            complement(7360..8406)
                     /locus_tag="OO172_003161"
     CDS             complement(7360..8406)
                     /locus_tag="OO172_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5513251.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8417..9412)
                     /gene="tssG"
                     /locus_tag="OO172_003162"
                     /pseudo
     CDS             complement(8417..9412)
                     /gene="tssG"
                     /locus_tag="OO172_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9409..11292)
                     /gene="tssF"
                     /locus_tag="OO172_003163"
     CDS             complement(9409..11292)
                     /gene="tssF"
                     /locus_tag="OO172_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5513252.1"
                     /translation="MDPRLLGYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRPLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11308..11802)
                     /gene="tssE"
                     /locus_tag="OO172_003164"
     CDS             complement(11308..11802)
                     /gene="tssE"
                     /locus_tag="OO172_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5513253.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11799..12629)
                     /locus_tag="OO172_003165"
     CDS             complement(11799..12629)
                     /locus_tag="OO172_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HCR5513254.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12616..13368)
                     /gene="tagK"
                     /locus_tag="OO172_003166"
                     /pseudo
     CDS             complement(12616..13368)
                     /gene="tagK"
                     /locus_tag="OO172_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3059 a         3488 c         3574 g         3247 t
ORIGIN      
        1 ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc gcacggtcgc
       61 tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc attaactcca
      121 ccaattatcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac gcaatcccgt
      181 tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa ccgtacttca
      241 cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa gcgggttcag
      301 tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc ccgtagcggg
      361 aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc ctggcacgta
      421 agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt tcgatcaaat
      481 taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt tcacggaaag
      541 gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg atccgtaaca
      601 gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc atctcctgca
      661 aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta agttgccgcg
      721 tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc gcccggctta
      781 ccgctgagtt tgcttgccct aaaattgttc cgctgcccgg ctaaagctgc cgctttcgac
      841 gaccgaaaca aaaatggcga gttcttccga cgtggctttc atttttgctc ctgttgcaaa
      901 atagatgaga tattttgaat ttatttgtca ttaaaccatc aggatgcgtg atatgtcatc
      961 cgatttaatg ttctcaataa tgagcaaatt tctgaccggt gtaagcactt gcttacataa
     1021 caatatacaa ttgctcgttg aaagagtgag ctaaaatccc tataacagta gaaccctccc
     1081 gagtgcggaa gggttgacgt aatagaggtt tcaaagtcaa aagtgcgaaa aaacacctat
     1141 gccatgcgct atgttgccgg acaacctgcg gaaaggatct taccgccggg gtcttttgcg
     1201 agcatcggcc aggcattacc acctggagaa ccgttaagta ccgaagagcg tattcggatc
     1261 ctggtgtgga acatatacaa acagcaacgc gctgaatggt tgtcggtatt aaagaactac
     1321 ggcaaagatg cacatctggt gttattgcag gaagcgcaga caacgccaga gttagtacag
     1381 tttgcgaccg ctaactatct tgccgccgat caggtgcccg ctttcgtgct gccacaacat
     1441 ccttccggtg taatgaccct ttcggcggcg catccagtgt attgctgccc gttacgtgaa
     1501 cgagaaccca ttttgcgtct ggcgaagtcg gcactggtga cggtctatcc attacctgac
     1561 acccgcctgt tgatggtggt taatatacac gccgtcaact tcagtctggg cgtggatgtc
     1621 tatagtaagc agttacttcc tattggtgat cagatagctc accacagcgg cccggtcatt
     1681 atggcgggag atttcaatgc ctggagccgt agaaggatga acgcgttata tcgctttgcg
     1741 cgggaaatgt cgctgcgcca ggtgcgtttt accgatgatc agcgccgtcg ggcgtttggc
     1801 cgcccgctcg attttgtttt ctaccgtggt ctgaacgtca gcgaagcttc tgtattggtt
     1861 acgcgcgctt ccgatcacaa tccgctactc gttgaattca gtcccggcaa gcctgataaa
     1921 taaggtatgt caggtctgcc acagggcaga ccaacgtttg gcgctgtcct tttatactca
     1981 aacaacaaaa ggacagcgca atgacaacac aatcccacca tgaccacgta gaaaagcagt
     2041 ttagctctca agcctgcgaa tatctgacca gtaccgtaca tgcatccgga cgagatttac
     2101 agcgcctggc ggtgcgtctg gctgattatc ctggtgcaag tgtacttgat atgggctgcg
     2161 gagcagggca tgccagcttt gttgctgcgc aaaacgtgag cgcggtggtg gcgtatgact
     2221 tatctgccca aatgctggat gtcgtggcac aagctgccga agcccggcaa ctgaaaaata
     2281 tcaccacccg ccagggatat gctgaaagtc tgccatttgc cgataacgca tttgatattg
     2341 ttatcagccg ttattctgcc catcactggc atgatgttgg tgcagcactg cgtgaagtga
     2401 accggatatt gaaacctggc ggtaggatga ttgtgatgga cgtaatgtct ccgggtcatc
     2461 cagtgcgcga catctggtta cagacggtag aagcattacg cgatacctct cacgtacgaa
     2521 actacgccag cggtgagtgg ttgaggttaa tcaatgaagc caatctgata gttgataatt
     2581 taattacaga taagttaccg ctggaatttt gttcatgggt cgcgagaatg cgtacgccag
     2641 aagcgttagt agacgctatt cgcatttacc aacagagcgc atcgacagag gtgagaacgt
     2701 attttgcctt gcagaatgac ggctctttca ccagtgatat catcatggta gaagcacata
     2761 aagcggcata aataaaaaag gcaccggggg aatcggtacc tttttattat ctggtttgtc
     2821 aggaatctgg catgttgttg tttttcacaa acaacgtcag cttgtcgcct ggttgcagat
     2881 tcgcagtgtc gctgttccag cgcatcacat ctttgatgtt cacgccgtgg cgtttagcaa
     2941 tgcttgaaag cgaatcgcct ttgcgcacac gatacgtaat gctatcgctg ttgtttgcca
     3001 gtcgctgtgc gctactgcct gcaccaatcg tcaaactttg gcctggcttc agcttagatc
     3061 cgcgcagttt gttccactgc tgcaaatctt tggtgcttac gccgagacgt gaagcgatgc
     3121 ttgaaagcgt gtcgccagag cgtacggtgt aaacacggct gttaagcggc gtattgtcgg
     3181 caaccagcgt cgactgtaca gcagcaattt cgcctgaagc gagagattca cgcagttgat
     3241 ctgcatgctt ctttggcacc atcacgtact gcggcccact tgcgcccagc gtggagcctt
     3301 tcacgccagc gttgaatgtc ttcagcttgc tgacggaaat ccccgccata tctgcaacct
     3361 tcgccatttc aaccgggcta ctcaggtgca cacgcgccag agcacggctt tcatcggtcg
     3421 ttggcagacg tacgccataa cgcttgctgt ttttgagaat atcactcaat gccagcattt
     3481 taggcacgta ctgcttcgtt tcctgcggca acggtaacga ccagaagtcc gtggatttcc
     3541 cacgcgcttt gttcgtttta attgccttca tgacccgacc ttcgccgctg ttataagccg
     3601 ctacggtcag aagccagtcg ccgtcaaaca ttttgttcag acgctgcatc atattcagcg
     3661 cggcagttgt tgaagcaaca acatcgcgac gcgcgtcata attgcgggtc tgtttcaaac
     3721 cataattgcg ccccgtgctc ggaatgatct gccagatgcc tgcggcattg gcgccagacg
     3781 ttgcgtgagg atcaaaagcg ctctccacta tgggtagtag taccagttcc ataggcatgt
     3841 tacgtttttt aacttgccct gctatccagt acatatacgg ctctgcccgt aaagttacat
     3901 cgtggagata gctcttattg cgtaaatatt tctgtttctg ttcgcgaatc cggtcatttt
     3961 ccggaattcc catctttagc tcgtcgccaa tgaaagccca caagtcacca tctggcgcga
     4021 tagacgtccc atcgtccatc catcgtgcct gacttgtaaa ctttgctgct tccccttgac
     4081 cagctgcaga aaggctctgt gcgtgctgtt gaacgttgcc ggtactctgg caacccacga
     4141 gcaggacaga ggcgagtaat atcgcttttg ccttcatgtg tgtgtcaata gttgcttaaa
     4201 agacgaccga tcataacggc gaacggagcc gatgacaaga aagttttatc agaacctatc
     4261 tttctttgac cttaaccatg caaaacgctc ttcaggttgt tgcaataatg tttcttcatt
     4321 aattacatta attaaatcaa tatcttccgt tcttaaaaaa acattaattt gccgctcatt
     4381 tttcagaatt acgggtagtg ttatttgatt ttttgcccgt aactccttaa ctttacgata
     4441 ataatcattt atggacaaat cgtgcggaaa aatactcaaa gcaaacttca tatttgataa
     4501 cgtatattca tgagcacaac ataccaatgt atcgtcaggt aacgcactta gctttttaag
     4561 tgattgatac atttgtaaag ctgtcccttc aaacaaccga ccacacccac cagaaaacag
     4621 cgtgtcgccg caaaatagat aaggtttact gaagtaacag atatgtccta aagtgtgacc
     4681 cggtgtggta attacactaa attcatgccc taaaacgaag gcagtttcgc catctttgac
     4741 tacctgtgtt gttcccttat cttgtgtctc ttgtggacca tacaccacaa tttgcggaaa
     4801 cttttccacc agttctttta cgccgccaac gtgatcgtgg tgatggtggg ttagaaatat
     4861 ggcctccggt tgccagttat tggcggtaat ggcgtttaat actggctctg cgtctccggg
     4921 atcgacaatc aggcagcgac ctgcctcatc attcaaaacc cagatgtaat tgtcatcaaa
     4981 ggcgggaata ctgttaagat tcatagatta cctctcagtg taaaacggaa ggttgtgatg
     5041 aaaccggcaa gagtccctca aactgtcgtg gctcctgatt gctggggcga tttgccctgg
     5101 ggagagcttt atcgcaaggc gctggagcgc cagctcaacc cgtggttcac taaaatgtat
     5161 ggttttcatc tgcttaagat tggcaattta agcgcagaaa tcaattgcga agcgtgcgcg
     5221 gtttctcatc aagtgaatgt ttctgcgcaa ggaatgcccg tccaggtgca ggcggatcca
     5281 cttcatcttc cttttgccga taaatccgtt gatatttgtc tactggcaca taccttgccg
     5341 tggtgcaccg atccgcatcg tttattacgt gaagccgatc gggtactgat tgatgatggc
     5401 tggctggtga ttagtggctt caaccccatc agtttaatgg gattacgcaa acttgtgccg
     5461 gtattgcgca aaacctcgcc ctataatagc cggatgttta ctctgatgcg gcaactggac
     5521 tggctctctt tgttgaattt tgaagtgctg cacgccagcc gtttccacgt tctcccgtgg
     5581 aacaaacacg gaggaaaact attgaatgcg catattcctg cgcttggttg cttacaactt
     5641 attgttgccc ggaaacggac tattccttta acgctaaatc cgatgaaaca gagtaaaaac
     5701 aagccacgaa ttcgccaggc ggttggagcc acccggcaat gtcgtaaacc acaggcttaa
     5761 acttcgactt ggtagcccgt atcttccagt gtgggattca tcgccgcggc acgagccagt
     5821 tcatcacagc gttcgttttc cgggtgtccg gcatggcctt taacccattc ccatttgatt
     5881 tgatgctgcc ccaatgcagc atcaagacgt tgccagagat cgacattttt tactggtttt
     5941 ttgtctgctg ttttccagcc acgttttttc cagttatgga tccactgggt gataccctgg
     6001 cggacatact ggctgtcggt actcaaaatg acttcgcaat gttcttttaa cgcctccagc
     6061 gcgacaatag cggccatcaa ctccatacgg ttgttggtgg tgcgggtgta gcctgcgcta
     6121 aaggttttct cgcgtccgcg atagcgtaaa atagcgccgt aacccccagg tccaggatta
     6181 cccagacacg aaccatcggt gaaaatttct acctgtttaa gcatctctgg tagacttcct
     6241 gtaattgaat cgaactgtaa aacgacaagt ctgacataaa tgaccgctat gagcactgca
     6301 attacacgcc agatcgttct cgataccgaa accaccggta tgaaccagat tggtgcacac
     6361 tatgaaggcc acaagatcat tgagattggt gctgttgaag tagtgaaccg tcgcctgacg
     6421 ggcaataact tccatgttta tctcaaaccc gatcggctgg tggatccgga agcctttggc
     6481 gtacatggta ttgctgatga atttttgctc gataagccca cgtttgccga agtagccgat
     6541 gagtttatgg actatatccg cggcgcggag ttggtgatcc ataacgcagc gttcgatata
     6601 ggctttatgg actacgagtt ttcgttgctt aagcgcgata ttccgaagac caataccttc
     6661 tgtaaggtca ccgatagcct tgcggtggcg aggaaaatgt ttcccggtaa acgcaacagc
     6721 ctcgatgcgc tatgtgcccg ctacgaaata gataacagta aacgaacgct gcacggggca
     6781 ttactcgatg cccagatcct tgcggaagtt tatctggcga tgaccggtgg tcaaacgtcg
     6841 atggcttttg cgatggaagg agagacacag cagcaacaag gtgaagcaac aattcagcgc
     6901 attgtacgtc aggcaagtaa gttacgcgtt gtttttgcga cagatgaaga gctggcagca
     6961 catgaagccc gtctcgatct ggtgcagaag aaaggcggaa gctgcctctg gcgggcataa
     7021 atacatgtaa aaggcgctaa aaatagcgac ttgggcgatt tttgcagcaa acgattcaaa
     7081 agatgagaaa aatcgttgac gaaggtcgag gcaatccgta gtattcgcct cgttcccaac
     7141 ggaacacaac gcggagcggt agttcagtcg gttagaatac ctgcctgtca cgcagggggt
     7201 cgcgggttcg agtcccgtcc gttccgccaa tattcagaaa gcccgaatca gtaatgattc
     7261 gggctttcgt cgttttggtg aagtgagaat ttttcactcc gaagtaaaac ggatatgatt
     7321 taaaaaatgg ccagacatta taaattctgg ctacttctgt tactccagcg catcccattt
     7381 ctccagcagt tctactggaa tttcatttat tacctccgag acgcgttttc cgaccaggcg
     7441 ttctgcctgg cgcagcaaag gaatggtcgg gctgctcggt tcgctgtgct caaaccagcg
     7501 acggatgaca cgcaggcgtt ccagcgcgtc gtttcggtcg cggatctgcc ggggttctgc
     7561 gccggtcatc ggtgatattt cctccgcgat atgcaccggt agcgcaatct gctctgcctg
     7621 catctgttca gtatgaacga tggtggcgtt ttccggctgc tcagcctgtg gttgtggcag
     7681 gatctccggc gtggtgctct gcactgctcc cggtaacagg gccagcagtt gcgtcagacg
     7741 ggagaagtcc ggtgcctggt cattcagcgt ttcacgcgca tggcgttgca gccgctcagt
     7801 ggtctctgct gcctggcgaa aggcatccag cggcagtgtg ccgcgcgctt ccaggtctgc
     7861 cagttgctgg cgtaccgatt ctggtgccag cgcatccgcc gggcgtaatg cagatagcga
     7921 acgctccaca tcccgcacct gaaggcgcat ggcagcatta tttgacagtg taatgccacg
     7981 aatatccgcc attacacctt cgtggtccag caacgccgcc agcgcgttac tgcgtgccac
     8041 cgcagcatct tcggcggtga tatcttcagt cgcgagcagt tgaggatgga tggcgtccgg
     8101 gtacgtggag cacaacgttt ccagcagcgt gagcgcctcc gacagtcccc tggcaccggc
     8161 ctgctggata cggcagcgca gcaggacaac cagaatgcgg atatctttgc tgcgcatcag
     8221 cagacgatgg gcgtcccgct caagctccgc ccagttaatg ttttccggcg tactgacaaa
     8281 atcgccatat tgcgcttctt cacgcggtgc ggcgcgggtg aacagcagca ggtattccgg
     8341 atcgtactcc agatccgggc cgcaggggca ttccgccgaa attgggtcag acaggaatat
     8401 ttccatttaa ttatcctcag tgcgccaggt agtgttctgg ttcgaaaatc atgcccgtca
     8461 ccgggttgtc gtgcatggct ttacccgccc atgcagtcca acccagttgg tacggaccac
     8521 cggtaacagc cggaggggcg gcgtggggct ttagctgtag ctcaatttcc cagcagtatt
     8581 cataaccact aaaggcgcgg accagttctg tcaggacggg caggttattg ctgccaggca
     8641 gaaaacgtcg gtaatcctcc agcgtcagcg ggccgataac cagccggaac ttatactgca
     8701 tgtccggcac ggcctggccg atgagtgcac tattgccaat aacggaagag ggggcaggca
     8761 tccccagccg ggtgatctca tcgtccgcca cggctatcca gtgcaggaca tactcctgta
     8821 tccggaaagg tacaccgaaa tagtgcgcca gcgtggtggc cagaccgtcc ggattgcggg
     8881 actcacgcac cagatgggcc gatgccgcca gacgagcgtg atctggcaac gggctgtcag
     8941 cgctctcccg caaatcctgc ccgctgaggc tggcgacata aaaggcaaag cggtcatgtt
     9001 gcggtttatc cagaccgccc ccagcggact gggcgctgcg ccatgcctgg taaaactgcg
     9061 tcagccagcg gtgatgaaaa agatcagaaa aacgggtcag cgtcggatcg cgtcggcttt
     9121 cagtccggtt acgcgccagt tcggtgtagt gcagcggcag tggaccgttc ggcccccaca
     9181 gcccgaggct gtacagactg atgtgcaggc gcccttcctg ccagcccaca ctggcgattt
     9241 ctcgtggcgc aaaggtcagc gcgggcgttt gtccgaggcg aaatttttcc atccgtggtt
     9301 gctaggtgtt gcccgccgga acggtacaca actgcacatc cacgcggcgc atcaggttca
     9361 gaaaaccgta atgccagggg ggttcctgcg cctgagtgaa atcgttgctc atacgcttcc
     9421 ccgctgtccc gccctgacag gccaggtcat aattttgccg cgctgcatgc tgtgcagcgt
     9481 catctgcgag aaggtattaa tggaaacatg gcgggcgata tagtgttcca gcaccaggcc
     9541 gaacagatac ggactgatgc cggaaaaacc ttcttcatcc accgtcagtt cacaactgac
     9601 gccacgccca tagaccagta gtccggaacc gggcagacgg cgggtgaccg gggtcgtttt
     9661 acaaccaatc aggctgcgca cctggcgcga ttgcggactg tcatgagcag gaataaacag
     9721 gttcagcaga tctcgcaacg cctggccgcc ggtacggtga tccagatcgg ccagcggcag
     9781 ataattaaaa gataactggc ggatcaaccg ccatgccatc tcacgttcag ccagcggggg
     9841 ctgcggcgag cgtggcgggc ggataagccc tacgcccgcc accgggatgg ctgcatccac
     9901 ggttagatcg tcccggccat tgcgggcaat aagacagggc agatcgcggt tagtgaccat
     9961 ggccgtgatg gtgatgtggc gcagattttc cgggtacggc gcttcatact ggtcaaccag
    10021 cgacaggaac acttccgagc ccgtataggg ggtacgggtg ccgtaacggc gggcgttttc
    10081 tgataagcgg cggggctccc ggcgcaggga aaaataacgt ccgtgattgc cttcatcatt
    10141 attgcgggtg tggtagagcg ggcgaaaggc catcttccgt gtggtgtcgg tttccagccc
    10201 ttccacttcc tggaccgaga acacctcata atccagcgga tgggttctgt ccaccaccag
    10261 atgctgttcc gtcgtgctgt gcgtgacatc gatccgggcg gtgacgcggg gaaacaggtt
    10321 gataaccggg gtacagaaca ggctgaactg tgccgcattt gtctggtgga tcagccagtc
    10381 cggcggcagg cggttcagca ggataacaat ctccgccacg ccaccgtcaa ttttctgtaa
    10441 tccggcagac aggcctgtcg gggtgaaaaa atagaagcgt tccggacagg caaaatattc
    10501 atgcagcagg ttatgaccgt gaaacacgtt ccacgccagc ggcagcagcc cctgatccgg
    10561 ctccaggcct tcgtacataa cgggctgttg cagattcaca tccagcgcgc cgtcaaaatg
    10621 ccccggaatg ccagccaggg tcgcgatggc gctggtatgt agaagttcta acaggtgtga
    10681 ggccgtacgc tcctcgccgc agagataaaa cggcagcctg tccagtccgg caagctgact
    10741 gaacgttagt tcaccaaagg tgcgcagggt aatacgcagc gccccggcga cgtgaatgtt
    10801 ggccggcagg tagcggtgca gggcaggcat atccggtggc gcggcggtca gtcgcgcctc
    10861 ttcgatggcg agcggccaca gcgttacatc ctggctgctg cgaaactggc aggcagtgct
    10921 ttcgccttcc ggaatggcgg agaaaaaggc tgtgtcgcgc gggacggtaa atcctttcgc
    10981 cagatcgcct tcttccgtat cgggatgcaa ctgcgccacg ctcattgagg gcgtgggtgt
    11041 gacgtaattg gggctgacca cttccagcgg gcgctgggtg aaacggggga attcagcgtc
    11101 aatttttagc tgcgtgcggg cggtcaggaa gctgaaggcc tcgaccattc gctctacata
    11161 cggatcggca atatccgttc cctgcatgcc cagccgggcg gcgactttgg gatgacgggc
    11221 ggcaaattcg gctcccgttt cccgcagata gctcagttcg cggttgtagt atcccagtag
    11281 ccgtggatcc atcggtctct ctctgaatta aaaaaacgta atgcgactct gctccatatc
    11341 cagcgcgcta cgtacccgga atgcagtcgg atacggctgc gtgaggatct cgccgcgaat
    11401 ttcaaactgt aatgtgttat ggccactctg gcggcgctca tcctgaagtg ggatgatttg
    11461 cagggtggac gctttcagcc ggggttcaaa ccgggtgatg gttcgcaaaa tggccctgcg
    11521 gatatcgtcc cacttgtgct catacagaaa gctgcccgcc agtggcggca ggccgtagtt
    11581 aagtacggac gctgccgcgt acggataacg tgcagcatca atatcatctt catggctgac
    11641 ggtgttgagc agaaatgaca aatcccggcg gatgatctcc ttaatctgaa ccgggctgac
    11701 gctgataccg tggtcgcgtt tctggtttgg ggcgtcgtca cacaggcggt caaacagcgt
    11761 gggcagcagg tgattgcctg gacgctggcg ggaagcgctc acgcgtcgct tccttcagcg
    11821 gtgttaaagg tacaggtgga caaatccagc aggctgatat cgccgtgacc ggtgagccac
    11881 accctctgac ccagcgcctg taccagtgtc tctcccggac cgtcctgcca gaccgtttcc
    11941 cggcacagac gcagggagtc ggcagccttt tcagagccgc tgtagcgggg aaacagccag
    12001 gcactgtgtg tatcgccatt gttcagggtg acgttgacgg gtttccagat caaatcgatc
    12061 agatgcgcag gccgcggcga tttcagcgaa cagatttgtg aaaacggcag ccagatatat
    12121 gctccgcccg cgatcagttc gagtaccgga ccggtacggg agtcgctgtc gctaatccag
    12181 tcaaatgcgc cgccattcca ctgcccggaa gtgtctgtta tggcctcaag cgcgcggctg
    12241 cggtgagcat cagcttcctg cgcttcacca cgggcgttac aggcgagggt ggtcagcaga
    12301 tcctccatcc aggcgggcgg cgggagaata acaccgggcc gctgttcgcc ctgaaagcag
    12361 gcgtggcggt atatctcaca gcggatcagc tcgccaaata cctgcgcttc gcgagtataa
    12421 tttgcgtcca tccgggcgca tagctggatc tgttgcagtg cccgcgacca gtcgcctgcc
    12481 acgcacaata actgaaacag gctgtggcgg cgggaggcct gcgctgggtg catcctgacc
    12541 tgttgctctg ccatctgaat gccctccacc acggaataat cccgcagcag cgccgacaag
    12601 gtaatcggta gctcgttatt gtttgtcatg atatatctct ccatgttgtg ccgggtttat
    12661 ccggtaatgg ttgtcaatgc caatgatgcg atgctctttc cgtgtcagat ctggcagtac
    12721 ggtgttatgc gctgtttccg gggcgtattc cggggaaagt aaatgcagga tatcaggggg
    12781 agtcttttcg acaggccaga tagtttcatc ttcgccagtg gcatccagta cattgagaat
    12841 agcgtcaatg cttaacccac cagtgaccat atcctgcaat gtctcggctt cggtgaccag
    12901 gtcacgcaat gaatccagat cctgcgagga gggctcttcg ttaaaaggaa aaggatcagc
    12961 atggcgatta agatcttgtt cgggagataa caacgcctcg cggtattcct gataaagtcg
    13021 ggtcagggca tttccttttt ccgcaccttc tccggtggag gaggctccat gaacaggaat
    13081 gatatcgaat ggattttgtg atgtcagatg ctgctgcctg aaccaatcca gatccagata
    13141 ttcggttatt gcttccgacg tgatggcagt ctgcgatatc gtttttactg tgctcaggtc
    13201 cggagatgat ggattgccat gtacaaggca ctatgaagat aacccccatt ctatcaggtc
    13261 gccttcattc agacgcatac ggtgatgagg ttccacaatc tggtcgttta ccgtacagca
    13321 atattcggta gactgattga taatccacca tgcttcttcg tggcgggt
//
LOCUS       DAJTNM010000108        12872 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTNM010000108 DAJTNM010000000
VERSION     DAJTNM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.108"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..393)
                     /gene="yafO"
                     /locus_tag="OO172_003167"
     CDS             complement(<1..393)
                     /gene="yafO"
                     /locus_tag="OO172_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5513255.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMR"
     gene            complement(396..689)
                     /gene="yafN"
                     /locus_tag="OO172_003168"
     CDS             complement(396..689)
                     /gene="yafN"
                     /locus_tag="OO172_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5513256.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(741..1796)
                     /gene="dinB"
                     /locus_tag="OO172_003169"
     CDS             complement(741..1796)
                     /gene="dinB"
                     /locus_tag="OO172_003169"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5513257.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1867..2652)
                     /gene="lafU"
                     /locus_tag="OO172_003170"
     CDS             complement(1867..2652)
                     /gene="lafU"
                     /locus_tag="OO172_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5513258.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2624..4336
                     /locus_tag="OO172_003171"
                     /pseudo
     CDS             2624..4336
                     /locus_tag="OO172_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4553..5050)
                     /locus_tag="OO172_003172"
     CDS             complement(4553..5050)
                     /locus_tag="OO172_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5513259.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTIQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(5226..5747)
                     /locus_tag="OO172_003173"
                     /pseudo
     CDS             complement(5226..5747)
                     /locus_tag="OO172_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            6131..6871
                     /locus_tag="OO172_003174"
     CDS             6131..6871
                     /locus_tag="OO172_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5513260.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6842..7609)
                     /locus_tag="OO172_003175"
     CDS             complement(6842..7609)
                     /locus_tag="OO172_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5513261.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(7815..8393)
                     /gene="lpcA"
                     /locus_tag="OO172_003176"
     CDS             complement(7815..8393)
                     /gene="lpcA"
                     /locus_tag="OO172_003176"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5513262.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            8633..11077
                     /gene="fadE"
                     /locus_tag="OO172_003177"
     CDS             8633..11077
                     /gene="fadE"
                     /locus_tag="OO172_003177"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5513263.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDTMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(11120..11566)
                     /gene="ivy"
                     /locus_tag="OO172_003178"
     CDS             complement(11120..11566)
                     /gene="ivy"
                     /locus_tag="OO172_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5513264.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            11747..12517
                     /gene="yafV"
                     /locus_tag="OO172_003179"
     CDS             11747..12517
                     /gene="yafV"
                     /locus_tag="OO172_003179"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5513265.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            12626..12858
                     /locus_tag="OO172_003180"
                     /pseudo
     CDS             12626..12858
                     /locus_tag="OO172_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         3051 a         3332 c         3413 g         3076 t
ORIGIN      
        1 acgcatgcga aacgcttctg ccatttttcc aattttatgc atttggttgt tatctcgagc
       61 cagtttatga ggttcaggct tcagaatggc aatgagcaac catgcttgct catcaaacgc
      121 accctgacaa tataccaaat gcgcttcgtc attcgttctg ctgaattggc gcaactgtgg
      181 cggaaatgga ttattcacat ttgccagatg aatatgagca actcgctcaa atttgattaa
      241 tggccaggta aacgagtcgt cgtagagtgc atcgcgacca aatatatctg gcaaaacacc
      301 gtcacgctta taggaaataa aatccgccgt taacgcatca agttcctctg ctgtaagttg
      361 caggcgaata agttttgttt tgaatacccg catccttatt ccttaaagtc attgaaatca
      421 tcatccgtca tatcattaag atattgttta gcgcggcgtg taatttcctc taatcttgca
      481 gcacttggac gaaagcgcgc ctttatgggc tttttctcct cttgttcagc aagcatgtcc
      541 attaacgctt cgaatgcgct ggcacttaag agatatcctg cggggcgatt attagaaaga
      601 accgcaactg gttgatcaat aaagtattta gctgggtttt tacgtaactc agtgatattg
      661 accgattttt cagcgagaat ccgatgcata cagtgatacc ctcataataa tgcacaccag
      721 aatatacata atagtataca tcataatccc agcaccagtt gtctttccat ttgcgggtca
      781 agcaacgtca catgcagccc caccagacgc acaccgcgcc cgccgcggcg ttcatcccag
      841 gttttacgcg cggtggcgat taagtcagct ttattcagcc gcggccagac gtgctcctgg
      901 gtggtctgct gaaaatcgtc gaactttaat ttcaccccct ggcgagcaat cagtaaatca
      961 ggttttacct ttgccagacg gcgttcaagt tccggataca gccgctcgat aatcgcttca
     1021 cattcagacc agtgatgaat atcttccgcc atcgtgcgtt ccacgccgac ggattttcgt
     1081 aaccgttcgc tgttaacgtc gcgctcgtca atcccctgac tacgctccca caaaatgcgg
     1141 ccaaatttgc caaagcgttt aagcagaatc accagatcac acttttgtac atcaccgcag
     1201 gtccgcagcc ccatcgcttc cagttttgcc gccgagactt tgccgacgcc ggggattttt
     1261 tccagtggta aggtttgtaa aaatgccgga acttctgccg gcgtaatcac aaactgaccg
     1321 ttgggtttat tcatgtcgga ggcgattttg gcgagaaact ttactggtgc cacgcccgca
     1381 gatgccgtca gttgcagctc gttgaagatc gtctggcgga tttcctgggc gatgagggtc
     1441 gcagaaccgt ggcaatggac gctatcggta acatcgagat aagcctcatc cagtgacaac
     1501 ggttcaatgc gcgaggtgta gcgcgagaag atttcacgga tatgatttga ggcttctttg
     1561 taggcgtcaa agcgccccgg aagcaaggtg agatgtgggc ataatttgag cgccatccca
     1621 gtaggcatag cgctacgtac gccaaattta cgcgcgggat aattggcggt gctgatcacc
     1681 ccccgacgtt cgcggctgcc gccaatagca atagggatat cgcgcagagc gggattgtcg
     1741 cgcatctcca ccgctgcgaa aaagcagtcc atatccacat gaatgatttt acgcattgct
     1801 cacctctcaa cactggtaaa gtatacagtg atttcaggtt ttgagaaatg cgtaaagatt
     1861 cagcatttag cgcattcgct gcgaaagcac ctgctgctta tccagctttt gcaccagcgg
     1921 ctgcaccact ttatccccat gctgaccaaa gtattgatac agcgtatcgg acgcactttt
     1981 ggtcagtacc ataatctcaa tacgccgatt gcccgcgctt tgcggattct tcgcgtccag
     2041 cagcatctgg tccgccattg cgcttacctg catcacttta tcttccggca ttccggcctc
     2101 ttccagcacc cgacgagctg aaagcgcgcg atcgcccgaa aggttccagt tgttgtagat
     2161 attgtttttg taggccatcg catcggtatg cccggtaata ataattttgt tatagagcga
     2221 gtcgaacact ggcgcgagct ccaccagcaa agttttaaag aacggcataa tctgcgcgct
     2281 gccgcgttcg aacatattgc gattctggtc gtctttaatc agcacgcgta atccctgggg
     2341 aacaatctcc atctccagat tcgcttccat atgcgcatca cgggcaatgg tgttgatact
     2401 ggttgccaac tcgccaagct cggtggcgga tttcttcttc agcagcgccg ttttctcatt
     2461 cacatcacgc gcttttttct ccgtctcttc cggcaccgcc acggtggcag gttttggcgg
     2521 atgagacggg ctgattttat ttaacggcga cagcccacca ccgttaaaaa tcgactgccc
     2581 gtgcagcgcg gcgataatgc tttcacgctc ggatttactg acgctgttga caatcaactt
     2641 tatcgtcgtc accaaagagg ccgagcgtat ttccgaggtt tctgcccgct ttaccctgga
     2701 cgcgatgccc ggcaaacaga tggcgattga cgccgatctt aacgccggat tgatcaacca
     2761 ggcgcaggcg caaacccggc gtaaagatgt tgccagcgag gccgatttct acggcgcgat
     2821 ggacggggca tcgaagtttg tacgcggaga cgccatcgcc gggatgatga ttctggcgat
     2881 caacctgagc ggcggcgtct gtatcggcat cttcaaatac aacctgagcg ccgacgccgc
     2941 cttccagcaa tacgtgctga tgaccatcgg cgacggcctg gtggcgcaga tcccttccct
     3001 gctgctctcc accgcggcgg cgattatcgt cacccgcgtc agcgacaacg gcgatatcgc
     3061 ccatgacgtg cgccaccagt tgctggcaag cccgtcggtg ctctacaccg ccaccgggat
     3121 catgttcgtg ctggcggtgg tgccggggat gccgcatctg ccgtttttgc tgttcagcgc
     3181 cctgcttggc tttaccggct ggcggatgag caaacgcccg caggcggcgg aggcggaaga
     3241 gaaaagcctc gaaacgctga cccgcaccat cactgaaacc agcgaacaac aggtcagttg
     3301 ggaaactatt ccgctgatcg agcccatcag cttaagcctc ggctacaagc tggtggcgct
     3361 ggtggacaaa gcgcagggca acccgctcac ccagcggatt cgcggcgttc ggcaggtgat
     3421 ttccgacggt aacggcgtgc tgctgccgga gatccgcatt cgggaaaact tccgcctcaa
     3481 gcccagccag tacgctattt tcatcaacgg cattaaggct gatgaagcgg atattccggc
     3541 ggataaactg atggcgctgc cctccagcga aacctacggc gagattgacg gcgtgctggg
     3601 gaacgacccg gcgtacggga taccggtcac ctggatccag cctgcgcaga aagcgaaggc
     3661 tctgaatatg gggtatcagg tgatcgacag cgccagtgtg attgccaccc atgtgaacaa
     3721 gattgtgcgc agctatattc ctgatttgtt taactatgat gacatcacgc agttgcataa
     3781 ccgtttgtcg tcgatggcac cgcgcctggc ggaggattta agcgcggcgc tcaattacag
     3841 ccaattgctg aaagtgtacc gggcgctgct gaccgaaggc gtttccctgc gcgatatcgt
     3901 caccatcgcc accgtgctgg tcgccagtag cacggtgact aaagatcata ttctgctggc
     3961 ggccgatgtg cgcctggcgc tgcggcgcag cattactcat ccgttcgttc gcaagcagga
     4021 gctgacggtg tatacgctga ataatgagct ggaaaatctg ctgaccaacg tagtgaatca
     4081 ggcgcaacag gccggcaaag tgatgctcga cagcgtgccg gttgacccga atatgctcaa
     4141 ccagttccag agcacgatgc cacaggtgaa agagcagatg aaagcggcgg ggaaagatcc
     4201 ggtgctgctg gtgccgccgc agctacgccc tttgctggcg cgttatgcaa ggttgtttgc
     4261 gccggggctg catgtgctgt cgtataacga agtgccggat gagctggagt tgaagattat
     4321 gggggcgttg atgtaagaat ccggcatcgg tgcacaattg ccggatgcgg cgtaaacgcc
     4381 ttatccggcc taccgttcag gcacaagttt ttaggcatga taagacgcgc cagcgtcgca
     4441 tcaggcatct gcgcataact gccggatgcg gcgtgaacgc cttatccggc ctaccgttaa
     4501 agcacgattt gtaggccgga taagcgtaag cgcatccggc accagcgcac agttataaaa
     4561 tgatacgctc cccggcactt aaatccgtta tgtctcccgc ccaatcgatg ggatataacc
     4621 ctcgcgcgac atcgcgatgg aacgttgaga atggccaatc actcacctgc tttacccagc
     4681 catgtttctc tggatttata taaatataat caacatgatg gcgataatct tttgtattgc
     4741 gaatggcatg ctcccaaaaa cgcggttgcc agatattttt caatccgcaa gcatgggtga
     4801 actgcttttt aatctcccgc cagcgagagg aaaaatcatc atcgctttcg ggtaatgtcc
     4861 atatgcaatg catatgctcc ggcaaaacga cccaggcgtt aatttcaaaa ggtctgtctc
     4921 gcttaacgtt aataatggcg ttacggagcg tctggaactg gatggtcaga agatggcttc
     4981 gacgattgcg taaatttacc gtgaaaaacc atgttccccc cttgatgtaa taacgacgat
     5041 attccgacat agcgactcct ttttctcaac ggtaagagga aaaggaaagg ccgacaatga
     5101 cgctgtgtta tatcgggaaa gcggctcgca aaaacgcaca gatttgtagg catgataaga
     5161 cgcgtcagcg tcgcatcagg catccgtgca caaccgccgg atgcggcgta aacgccttat
     5221 ccggcctaca aaatcatctc ttccgtcaaa atcctgcgtg cgcctaaaaa atggtcctgc
     5281 cagaaaggtt cggctaaccg gcttacccga atggtttccc cggtgcgcag tgactcaata
     5341 aactgaccgt cgcccagata cacccccata tggtcggcaa tctcgcggct gtggatatgg
     5401 aaaaatagca aatctccccg gcgcaggtcg ttattcgcca caatcgttgc ccgacggtag
     5461 tggtacatct cattagccgt gcgcgggagt ttcgcctcaa ggatcttgtt gtaggcataa
     5521 aaaactaacc cactacaatc gaagccttga tcgggccgcg tgccgcccca gacgtaaggc
     5581 ttgccaagct gctgctccag gcggtgaatc gccacttcgg tgatgttatg caggtgatcg
     5641 ctgcttaaaa agtggttgtt ttccgccagc tcctgccagc gtctgtcact cgccttgagc
     5701 ggcgcaggaa accattctgg atgctgtttt atctgctccc ggttgtggtg gcgctggcga
     5761 gtaaagggag caaaacgcat aacgaaaaca cgcctggatg caaaaatctg ccgagcaaga
     5821 acgaagacat gaaagacata aaccggctga atgaaggtga gaattttaaa agaccggagg
     5881 agtaaacggg attattaatt gcttatttgc atattactat tgagatttca taatattagg
     5941 aaataaatga tacatgcgct tttcggacta ttgaaaactg cccctgtgct aaatttgtaa
     6001 acccagcgcc gacaggcttg cgtaaaactc ttttggtgat aatgtgagcc ttccaaatcc
     6061 agactattcc gatttgggag ccattttgcc gtcctggcgt gtgtaaccgt tttatcaagg
     6121 aataagcagt atgcgtaaaa tcgcattaat tcttgcgatg ttgttgatcc cgtgcgtttc
     6181 gtttgccggt ttgctgggca gcagtagctc gaccacgcca gtgagcaaag agtataagca
     6241 gcagttgatg ggatcccctg tctacatcca gatcttcaag gaagaacgta cgctcgatct
     6301 ttacgtcaaa atgggcgagc aatatcaact gctcgacagt tataaaatct gtaaatattc
     6361 cggcggctta gggccaaaac agcgtcaggg cgatttcaaa agcccggaag ggttttatag
     6421 cgtccagcgt aatcagttaa aaccagacag ccgttactac aaagcgatta atattggatt
     6481 ccccaatgct tatgaccgtg cgcatggtta tgaagggaaa tacctaatga tccacggcga
     6541 ttgtgtatcc atcggctgct acgcaatgac caatcagggt attgatgaga tcttccagtt
     6601 cgttactggt gcgctggtgt ttggtcagcc gagcgtgcag gtgagtattt acccattccg
     6661 catgaccgac gccaatatga agcgccataa atattccaac tttaaggact tctgggagca
     6721 gctgaagccg ggctacgact actttgagca aacccgtaag ccaccgaccg tttctgtggc
     6781 caatggccgt tacgtggtca gcaagccgtt aagccacgaa gtggtgcagc cacaactggc
     6841 atcaaactac acgctccccg aggcaaaata agcgccattc gcctggcata attttttgcc
     6901 aggtttcatt gcccgtcagc ggctgtgtcg caatcaccgt gaccacatca ttcggtgtgg
     6961 tctgcgagct gaagtcgatt tccacatcct gatccagcaa cgttgccacg ccaaacggcg
     7021 cgcggcgggt gatccagtgt aaattagtcg agcaatacgc cattacatag cgcccgtccg
     7081 aaagcagcat gttgaaaacg cccttcgccc gcagttcatc cgccagtgag gcgatatatt
     7141 taaatactgc cgccatgttg cccggcgtgc gcgggtaacg ctgcgttaat ttatgcagca
     7201 gccagcaaaa ggctttttcg ctgtcggttt caccgaccgg gcggaagttg ccggtttcca
     7261 gtgatttgta gcccgtcagt tgtccgttat gggcataagt ccagttacgc ccccataact
     7321 cgcgggtaaa tgggtgagta ttttccagcg ccacctcgcc ccgattagcc tggcgaatat
     7381 gagccaccac cgaacaggat tttatcgggt agtcctggac aagtttggcg atgggggaat
     7441 taaagctggg ttgtggatct ttaaatgtgc gacagccttt accttcgtaa aaggtaatgc
     7501 cccagccatc tttatgtggc ccggttccac caccacgctg tacaagtccg gtgaaactaa
     7561 agcagatatc ggtagggacg ttggcgctca tcccgagcaa ttcgcacatg ggtaacctcc
     7621 acaacagcgg gagcgcatgg accccggcga agattgattt aagaattttc aggtcggatg
     7681 cggcgtaaac gaattattcg gcctacggtt cgggtacaga tttgtaggca tgataagacg
     7741 cgtcagcatc gcatcaggca tcagcgcaca agtgccggat gcggcgtaaa cgccttatcc
     7801 ggcctacgcc agacttactt aaccatctct ttttcaatta actggatcag gatatggatc
     7861 actttaatgt gaatctcctg aatgcggtcg gcataaccaa agtgcggaac gcgaatttcg
     7921 atatccgccg tgccagccat tttaccgccg tctttaccgg tcagggtgat cactttcatc
     7981 cccttctcac gcgccgctgc gatcgctttg atcacgtttg cagagttacc ggaggtggag
     8041 atccccagca gtacatcgcc ttcgcgacct accgcttcaa cgtagcggga gaaaatatca
     8101 ttgaaaccga aatcattacc gacgcaggaa atatgactaa cgtcagaaat agcaatcgcc
     8161 gggtagcccg gacggttttc acggtagcga ccggtcaact cttcggcaaa gtgcatagcg
     8221 tcgcaatggg aaccgccgtt gccgcaggaa agcactttgc caccggcttt aaagctgtct
     8281 gctaacagga ccgccgcgcg ctgaatggcg tgaatattgg cgtcatcttt taaaaagtta
     8341 gccagcgttt ccgccgcttc gttcagttcg ttacgaataa gatcctggta catgaggata
     8401 tccttcagca taaatgtaat agacaaaatg cagtgtaccg gataccgcca aaagcgagaa
     8461 gtacgggcag gtgctatgac caggactttt tgacctgaag tgcggataaa aacagcaaca
     8521 atgtgagctt tgttgtaatt atattgtaaa cacattgcta aatgttttta catccactac
     8581 aaccatatca tcacaagtgg tcagacctcc tacaagtaag gggcttttcg ttatgatgat
     8641 tttgagtatt ctcgctacgg ttgtcctgct cggcgcgttg ttctatcacc gcgtgagctt
     8701 atttatcagc agtctgattt tgctcgcctg gacagccgcc ctcggcgttg ctggtctgtg
     8761 gtcggcgtgg gtactggtgc ctctgaccat tatcctcgtg ccatttaact ttgcgcctat
     8821 gcgtaagtcg atgatttccg cgccggtatt tcgcggtttc cgtaaggtga tgccgccgat
     8881 gtcgcgcact gagaaagaag cgattgatgc gggcaccacc tggtgggagg gcgacttgtt
     8941 ccagggcaag ccggactgga aaaagctgca taactatccg cagccgcgcc tgaccgctga
     9001 agagcaagcg tttctcgacg gcccggtaga agaagcctgc cggatggcga atgatttcca
     9061 gattactcat gagctggcgg atctgccgcc ggagttgtgg gcgtacctta aagagcatcg
     9121 cttcttcgcg atgattatca aaaaagagta cggcggactg gagttctcag cttatgccca
     9181 gtctcgtgtg ctgcaaaaac tctctggcgt gagcgggatc ctggcgatta ccgtcggcgt
     9241 gccaaactca ttaggcccgg gcgaactgtt gcaacattac ggcactgacg agcagaaaga
     9301 tcactatctg ccgcgtctgg cgcgtggtca ggagatcccc tgctttgcac tgaccagccc
     9361 ggaagcgggt tccgatgcgg gcgcgattcc ggacaccggg attgtctgca tgggcgaatg
     9421 gcagggccag caggtgctgg ggatgcgtct gacctggaac aaacgctaca ttacgctggc
     9481 accgattgcg accgtgcttg ggctggcgtt taaactctcc gacccggaaa aattactcgg
     9541 cggtgcagaa gatttaggca ttacctgtgc gctgatccca accaccacgc cgggcgtgga
     9601 aattggtcgt cgccacttcc cgctgaacgt accgttccag aacggaccga cgcgcggtaa
     9661 agatgtcttc gtgccgatcg attacataat cggcgggccg aaaatggccg ggcaaggctg
     9721 gcggatgctg gtggagtgcc tctcggtagg ccgcggcatc accctgcctt ccaactcaac
     9781 cggcggcgtg aaatcggtag cgctggcaac gggcgcgtat tctcacattc gccgtcagtt
     9841 caaaatctct atcggtaaga tggaagggat tgaagagccg ctggcgcgta ttgccgggaa
     9901 cgcttatgta atggatgctg cggcatcgct gattacctac ggcattatgc tcggcgaaaa
     9961 acctgccgtg ctgtcggcta tcgttaagta tcactgtacc caccgcgggc agcagtcgat
    10021 tattgatacg atggatatta ccggcggtaa aggcattatg ctcgggcaaa gcaacttcct
    10081 ggcgcgtgct taccagggcg caccgattgc catcaccgtt gaaggggcta acattctgac
    10141 ccgcagcatg atgatcttcg gacaaggagc gattcgttgc catccgtacg tgctggaaga
    10201 gatggaagcg gcgaagaaca atgacgtcaa cgcgttcgat aaactgttgt tcaaacatat
    10261 cggtcacgtc ggtagcaaca aagttcgcag cttctggctg ggcctgacgc gcggtttaac
    10321 cagcagcacg ccaaccggcg atgccactaa acgctactat cagcacctga accgcctgag
    10381 cgccaacctc gccctgcttt ctgatgtctc gatggcactg ctgggcggca gcctgaaacg
    10441 tcgtgagcgt atctcggcac gtctggggga tattttaagc cagctttacc tcgcctctgc
    10501 cgttctgaag cgttatgacg acgaaggccg taatgaagcc gatctgccgc tggtgcactg
    10561 gggcgtacag gatgcgctgt atcaggcaga acaggcgatg gacgatttac tgcaaaactt
    10621 cccgaaccgc gtggttgccg ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta
    10681 tctggcaccg tctgacaagc tggatcataa agtggcgaag attttacaag tgccgaacgc
    10741 cactcgttca cgtattggtc gcggtcagta cctgacgcca agcgagcata acccggttgg
    10801 tttactggaa gaggcgttgg tggatgtgat tgccgccgac tcgattcatc agcggatctg
    10861 taaagagctg ggtaaaaatc tgccatttac ccgtctggat gaactggcgc acaacgcgct
    10921 gacgaagggg ctgattgata aagatgaagc cgctattctg gtgaaagcgg aagaaagccg
    10981 tctgcgcagt attaacgttg atgactttga tccggaagag ctggcgacga agccggtaaa
    11041 gttgccggag aaagtgcgga aagttgaagc cgcgtaattg aataacggag ccgaaaggct
    11101 ccgtttcttt atccgctaat tatttaaaat taaagccatc cggatggttt tccaggctgc
    11161 cggtcaacgc cgcgaacagc accgttttac catcaatcga aagcgcatcg ttcacattca
    11221 gccaggtgag tttctcttgc gacgttttct catcaatagc cgagaacagc cccgtcatct
    11281 gattagattt ctcggaccac atcacagcga tacgttgcga gccacagtca tgcggtttgc
    11341 acgcgctcat cacctgatac gtctcatctc ccaacgttac ggtttgtgcg ggagtataag
    11401 taccgccttt catcacccat gcaggtagct tatgcccttg caccatctga ttaaacgcag
    11461 ctttggtggt ttcgcccttt gcaaggctgc taatggttaa atcatcctgc gccattgcac
    11521 tggtggcgat gaccagtgcg gcgactgtcg ttattgcctt aaacatcatt cctcccgagc
    11581 ttatcctgcc catgttatta acctcctaaa gcctggcgat aacacgataa tttgcaaatc
    11641 agatatttcc aatatcagca tttgttatca atttattctg cgctaaggca actgcctgat
    11701 aaggtgttaa agttataaaa cccttcgcat aaaggagttg tacaccgtgc ctagtttgaa
    11761 gattacgctt ttgcagcaac cgttggtgtg ggtggatggt cctgccaacc tgcgccattt
    11821 tgatcgtcaa ctggaaggta ttaccgggcg cgatgtgatc gttctaccgg agatgtttac
    11881 cagcggcttt gccatggaag cggcagcttc gtcgcttgca caagatgacg tagtgaactg
    11941 gatgacagct aaggcgcagc agtgcaatgc gctgattgca ggcagtgttg cattacaaac
    12001 ggagtctggt tcggttaacc gctttttgct ggttgagccg ggcggcacgg tacattttta
    12061 tgataagcgt catctgttcc gcatggcaga tgagcatctg cattataaag cgggcaatgc
    12121 gcgagtgatt gtggaatggc gcggctggcg tattttgccg ctggtgtgct acgacttacg
    12181 ttttccagtg tggtcgcgca atctcaacga ttatgacctc gccctgtacg tcgccaactg
    12241 gccagctccg cgctctctgc actggcaggc attgctgacg gcccgcgcga ttgagaatca
    12301 ggcgtatgtg gcgggatgca atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg
    12361 tgacagccgg gtgattaatc cgcaaggcga gattatcgct accgccgacg cgcataaggc
    12421 aacgcgtatt gatgcggagc tgtcgatggt ggcattgcgg gaatatagag aaaagtttcc
    12481 ggcatggcgg gatgcggatg agtttaggtt gtggtgagtg ggggttgtgt ttagggccgg
    12541 ggctgggcta gattacgaaa gcccctaact taaacacaat attacgccag aagtcaccat
    12601 ttccaaaaca cagcgtcgct gtaccgtggg ttatctgccc aaccgcagcg acacgccggt
    12661 gatcaaaatc agcgccaaat ggctgagaaa agcgggattt gagatcggca cgggcgttac
    12721 cgtgaagatc tcggagggat gtttgatcct gcttgggata acaacgaagt acaggaactg
    12781 cgcagggaac tttatcaggt taaacagtca gttaagggga tgcgagacgg gatgtttagt
    12841 gtgctgaacg aaagttaaat aataaaaagc cg
//
LOCUS       DAJTNM010000109        12755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTNM010000109 DAJTNM010000000
VERSION     DAJTNM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.109"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(107..745)
                     /gene="rutR"
                     /locus_tag="OO172_003181"
     CDS             complement(107..745)
                     /gene="rutR"
                     /locus_tag="OO172_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5513266.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1033..2124
                     /gene="rutA"
                     /locus_tag="OO172_003182"
     CDS             1033..2124
                     /gene="rutA"
                     /locus_tag="OO172_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5513267.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2124..2816
                     /gene="rutB"
                     /locus_tag="OO172_003183"
     CDS             2124..2816
                     /gene="rutB"
                     /locus_tag="OO172_003183"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5513268.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGDYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIHHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DTLSPTSFARIA"
     gene            2828..3214
                     /gene="rutC"
                     /locus_tag="OO172_003184"
     CDS             2828..3214
                     /gene="rutC"
                     /locus_tag="OO172_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5513269.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGLVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3222..4022
                     /gene="rutD"
                     /locus_tag="OO172_003185"
     CDS             3222..4022
                     /gene="rutD"
                     /locus_tag="OO172_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5513270.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAVRAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4032..4622
                     /locus_tag="OO172_003186"
     CDS             4032..4622
                     /locus_tag="OO172_003186"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5513271.1"
                     /translation="MNEAVSPGVLSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4633..5127
                     /gene="rutF"
                     /locus_tag="OO172_003187"
     CDS             4633..5127
                     /gene="rutF"
                     /locus_tag="OO172_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5513272.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5148..6476
                     /gene="rutG"
                     /locus_tag="OO172_003188"
     CDS             5148..6476
                     /gene="rutG"
                     /locus_tag="OO172_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5513273.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6558..6665)
                     /locus_tag="OO172_003189"
     CDS             complement(6558..6665)
                     /locus_tag="OO172_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513274.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7038..7634
                     /gene="wrbA"
                     /locus_tag="OO172_003190"
     CDS             7038..7634
                     /gene="wrbA"
                     /locus_tag="OO172_003190"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5513275.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7655..7882
                     /locus_tag="OO172_003191"
     CDS             7655..7882
                     /locus_tag="OO172_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5513276.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7920..9161)
                     /gene="agp"
                     /locus_tag="OO172_003192"
     CDS             complement(7920..9161)
                     /gene="agp"
                     /locus_tag="OO172_003192"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5513277.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9454..10710)
                     /locus_tag="OO172_003193"
     CDS             complement(9454..10710)
                     /locus_tag="OO172_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5513278.1"
                     /translation="MGSNIHGMSCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10971..11891
                     /gene="cbpA"
                     /locus_tag="OO172_003194"
     CDS             10971..11891
                     /gene="cbpA"
                     /locus_tag="OO172_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5513279.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11891..12196
                     /gene="cbpM"
                     /locus_tag="OO172_003195"
     CDS             11891..12196
                     /gene="cbpM"
                     /locus_tag="OO172_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5513280.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12289..12705)
                     /gene="torD"
                     /locus_tag="OO172_003196"
                     /pseudo
     CDS             complement(12289..12705)
                     /gene="torD"
                     /locus_tag="OO172_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
BASE COUNT         3050 a         3245 c         3212 g         3248 t
ORIGIN      
        1 tgaacgttcg ctgagtgtga ataccgctgc cgctggcggt aacgccagtt taatgacgat
       61 cggttaaaca ggtagccgga ggatgttaca acctcctccg gcatctttaa cgtggtcgaa
      121 tcccctcaat aataatccgc tgcacgtttt caaccgtttg attgaaaaat acctcatcgc
      181 gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg
      241 aagcccaaat cataaaaatc aaatgctgcg gatcaatcgg cgcgagtttg ccgcttttga
      301 cccaaccggc aatcagcgcc gatttctcat caattaatgc cttcaaatcg cccgtcagtt
      361 catccattaa caacggcgcg cctgccagca tctccataca gaacaggcgc gaagcctgcg
      421 gataatcgcg tgagacttcc agcttcagac ggatgtactc ttttatcgcc gccagcgggg
      481 cgaaatcttc acgaaacgct tttaacggtg ccagccagat atcgagaatc tgccgcagca
      541 cggcaatata cagcgcctct tttgacggga agtaatacag cagattggtt tttgaaacac
      601 ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa accgaattgt gaaaaagtgt
      661 ccagtgctgc gctaagaatc gcttttttct tcgcgcttac tgcgcgcgaa cgtttacccg
      721 ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt gtagattggt aacaggatag
      781 caaaaccccc gcccggcctc gaccttctgc actctcatcg cgcacggatg catgttttat
      841 gtgcaactgt tttgaccgtt tagtccactt tttaccagat attttagata acacaatgct
      901 aacacattaa taacattcac tttttaaaaa ctggcatccg ctttgcaaac aagccaatac
      961 gcggctgaaa aggatatgca ggatgcagcg ccccgtctga ctttcacttt acgagacgaa
     1021 gagaggttga tgatgaaaat tggcgtattc gtacctattg gcaacaacgg ctggctcatt
     1081 tcgacccacg cgccgcagta catgccgacc tttgaactga ataaagccat tgtgcaaaaa
     1141 gcggagcatt accatttcga tttcgccctg tcgatgatca aactgcgtgg ctttggcggc
     1201 aaaactgagt tctgggatca caaccttgag tcgttcacct tgatggcggg tctggcagcc
     1261 gtgacctcgc gcattcagat ttacgccact gctgccacct taacgttacc tccggcaatc
     1321 gtcgcccgta tggccgcaac catcgactcc atctctggcg ggcgttttgg cgtcaacctc
     1381 gtgactggct ggcaaaagcc cgagtatgag cagatgggta tctggcctgg cgatgactat
     1441 ttctcccgtc gttacgacta tctcaccgaa tatgttcagg tgctgcgcga cctgtggggc
     1501 acggggaaaa gcgattttaa aggcgatttt ttcaccatga atgattgtcg cgtcagtccg
     1561 caaccgagta tccccatgaa agtgatctgc gccgggcaaa gcgacgctgg catggcgttc
     1621 tccgcccagt atgccgattt caacttctgt ttcggcaaag gcgtaaatac acccacggct
     1681 ttcgccccga ccgctgcgcg gatgaaacag gccgcagagc aaaccggacg cgacgttggc
     1741 tcttatgtgt tgtttatggt gattgccgat gaaaccgacg atgccactcg cgccaaatgg
     1801 gaacactaca aagcgggcgc ggatgaagag gcgttaagct ggctaaccga acaaagtcag
     1861 aaagataccc gctccggtac tgacaccaac gttcgtcaga tggccgatcc cacttcggcg
     1921 gtaaacatca atatggggac gttagtcggt tcttacgcca gtgtcgcgcg catgttagat
     1981 gaagtcgcaa gcgtgcctgg tgcagaaggc gtgctgttga ccttcgatga ttttctgtcg
     2041 ggaatcgaaa ccttcggcga gcgcattcaa ccactgatgc agtgccgcgc ccatctctct
     2101 gcgctgactc aggaggtggc atgatgacaa ccttaaccgc tcgaccggaa gccattacct
     2161 tcgatccgca gcaaagtgcg cagatcgtgg tggatatgca aaacgcttat gccacgccag
     2221 gcgactactt agatctcgcc gggtttgatg tctcaaccac tcgcccggtc attgccaaca
     2281 ttcaaaccgc tgtgaccgca gcgcgagcgg cagggatgtt gatcatctgg tttcaaaatg
     2341 gctgggatga acagtatgtc gaggctggcg gccccggctc accgaatttt cataaatcga
     2401 acgccctgaa aaccatgcgt aagcagccgc agctgcaggg gaaattgctg gcgaaaggct
     2461 cctgggatta tcaactggtg gatgaactgg tgccgcagcc tggcgatatt gtgctgccga
     2521 agccgcgcta cagcggtttc ttcaatacgc cgctggacag cattttgcgc agccgtggaa
     2581 tacaccatct ggttttcacc ggtatcgcta ccaacgtctg cgtcgaatcg acgctacgcg
     2641 atggcttttt tctggagcat ttcggcgtgg tgctggaaga cgcaacacac caggcggggc
     2701 cggaatttgc acagaaagcc gcgttgttca atatcgaaac cttttttggc tgggtcagcg
     2761 acgtcgaaac gttctgcgac acgctttctc ccacgtcctt tgctcgtatc gcttaaggag
     2821 tttaacgatg ccaaaatccg taattattcc cgctggcagc agcgcaccgc tggccccctt
     2881 cgttcccggc acgctggctg atggcttggt gtatgtctcc ggtacgctgg cttttgatca
     2941 acataataac gtgctgtttg ccgatgaccc aaaggcgcaa acccgccacg ttctggaaac
     3001 tatccgcaag gtgatcgaga cggcgggtgg cacgatggcg gatgtgacct tcaacagcat
     3061 ctttattacc gactggaaaa attacgccgc gattaacgaa atctacgccg agttttttcc
     3121 gggtgataaa ccggcgcgat tctacattca gtgcggactg gtaaaacctg acgcgctggt
     3181 ggaaatcgcc acaattgcgc atatcgccaa gtgaggccgc gatgaaactt tcactctcac
     3241 ctccccctta tgctgatgca cccgtagtgg tgttgatttc gggtcttggc ggtagcggca
     3301 gttactggtt accgcaactg gcggtgctgg agcaggagta tcaggtagtc tgttacgacc
     3361 agcgcggcac cggcaataat cccgacacac tggcagaaga ttacagtatc gcccagatgg
     3421 cagcggaact gcatcaggcg ctggtagccg cagggattga gcgttacgcg gtggtcggcc
     3481 atgcgctcgg tgcgctggtg gggatgcagc tggcgctgga ttatcccgcg tcggtaacta
     3541 tgctggtcag cgttaacggc tggctacgaa taaacgccca tacgcgccgc tgttttcagg
     3601 ttcgcgaacg attactgtat agcggcggcg cgcaagcatg ggtggaagcg cagccgttgt
     3661 tcctctatcc cgccgactgg atggcggtcc gcgcacctcg cctggaggca gaagacgcgc
     3721 tggcactggc gcattttcag ggcaaaaata atttactacg tcgacttaac gccctcaaac
     3781 gcgctgactt tagtcaccat gcggatcgca tccgctgccc ggtgcaaatc atctgcgcca
     3841 gtgatgatct gctggtgcca acagcatgtt ccagtgaact tcatgccgcc ctgcccgata
     3901 gccagaaaat ggtgatgccc tatggcggac acgcctgcaa cgtgaccgat cccgaaacgt
     3961 ttaatgctct gttactcaac gggcttgcca gcctgttaca tcaccgtgaa gccgccctgt
     4021 aaggaattgc tatgaacgaa gccgttagcc caggtgtgct tagcaccctg ttcaccgatg
     4081 cccgcactca caacggctgg cgggagacac ccgtcagcga tgagacgtta caggagcttt
     4141 atgccctgat gaaatggggg ccgacatcag ctaactgttc tccggcacgg atcgtgttta
     4201 tccgcactgc agaaggaaaa gaacgtctgc gcccggcgct ttccagcggc aatctgcaaa
     4261 aaaccctgac cgcgcccgtc accgctatcg tcgcttggga cagtgaattt tatgaacggt
     4321 taccactact gtttccccac ggtgatgccc gcagttggtt tacctccagc ccacaacttg
     4381 ccgaagaaac agcgtttcgc aacagttcca tgcaggcggc ctatctgatc gtcgcttgcc
     4441 gtgcgctggg actggatacc ggcccgatgt cgggttttga ccgtcaacac gttgacgacg
     4501 ccttttttgc gggcagcacg ctgaagagca atctgctgat taatatcggc tatggcgata
     4561 gcagcaaact ttttgcgcgc ctgccacgtc tgtcctttga agaagcctgc gggctgttgt
     4621 aaggagcaaa ctatgaacat agttgatcaa caaacctttc gcgatgcgat gtcctgtatg
     4681 ggcgcggcgg tcaatatcat caccacggac ggtccagccg ggcgcgccgg gttcaccgcc
     4741 agcgccgtct gcagcgtgac cgacacgccg cccacattac tggtgtgcct gaatcgtggg
     4801 gcgtccgtct ggccggtatt caatgaaaat cgaacgctgt gtgtaaatac actgagcgcc
     4861 gggcaggagc cgctttcaaa tctttttggc ggcaaaacgc ccatggaaca ccgctttgcc
     4921 gccgcccgct ggcagacggg tgtgaccgga tgcccgcaac tggaagaggc tctggtttcg
     4981 tttgactgcc gtatcagcca ggtggtgagc gtcggcaccc acgacattct gttttgcgcc
     5041 atcgaagcga ttcatcgtca caccacaccc tacgggctgg tgtggttcga tcgcagttat
     5101 cacgcgctga tgcgccctgc ttgttaacct ccttaaggag acagctgatg gcaatgttcg
     5161 gttttcctca ctggcagtta aaatcgacct ctacagagag cggcgtggtc gcgccggatg
     5221 aacgattgcc gtttgctcag acggcagtaa tgggcgttca acacgcggtg gcgatgtttg
     5281 gcgcgacggt gttaatgccg attctgatgg ggctggatcc caatctttcc attttaatgt
     5341 cggggatcgg cacgttactg tttttcttta ttaccggcgg gcgcgtcccc agttatctcg
     5401 gctccagcgc ggcttttgtc ggcgtggtga ttgccgcgac gggctttaac ggtcagggca
     5461 ttaacccgaa tatcagcatc gcgctgggcg ggattatcgc ctgtgggctg gtttataccg
     5521 tcattggcct ggtggtgatg aaaattggta cacgctggat tgaacgacta atgccgccgg
     5581 tggtgacggg tgcggtggtg atggcgattg gcctgaactt agcgcctatc gcagtgaaaa
     5641 gtgtatcggc ttcggccttt gatagctgga tggcggtaat gacggtgctg tgtattgggc
     5701 tggtggcggt gtttacccgt ggcatgatcc agcggttatt gattctcgta ggattaattg
     5761 tcgcctgtct gctgtatggc gtgatgacca acgttttagg gctgggaaaa gccgtcgatt
     5821 ttactctcgt cagctatgct gcctggttcg gtctgccgca tttttccact cctggtttta
     5881 atggccaggc gatgatgttg attgcgcccg tggcggtgat tctggtggcg gaaaacttag
     5941 gtcatctcaa ggcggtcgcc gggatgaccg gacgcaatat ggacccgtat atggggcgag
     6001 cattcgtcgg tgatggtctg gcaacgatgc tttccggctc tgtcggcggt agcggcgtca
     6061 ccacctatgc tgaaaatatc ggtgtgatgg ccgtgacaaa agtctactcg acgctggtgt
     6121 ttgttgcagc tgccgtcatc gctatgctgt tgggcttttc gccgaaattt ggcgcgctga
     6181 tccataccat tccggccgcg gtgattggtg gtgcatcaat tgttgtgttc gggctgattg
     6241 ccgtcgcagg cgcaaggata tgggtacaaa accgtgtcga tttaagccag aacggtaatt
     6301 tgattatggt cgcagtaacc ttcgttctgg gcgcgggtga ttttgctctc acgcttggcg
     6361 gttttacgtt gggagggatc ggtacagcaa cctttggcgc gattttactc aatgcgttat
     6421 taagcagaag gttggttgac gttccaccgc cagaagttgt tcatcaggag ccataaacag
     6481 aaaaccgccg ggttaccggc ggtgatgact ttttgacgag cttaagaaaa aaatgcattc
     6541 gtcattgatt agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta
     6601 cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc tcgatggttt
     6661 gccattactg acctccgtct gggtatagac atcatattgc atagaattaa cgcggcgtat
     6721 ggcggctgcc atattttcgc tacgtgggac taagcttagt gaactgacgc gggcagaccc
     6781 gcgtttggta atatcctgca acagcttctt agcgagaata gatatcatct ccaataatta
     6841 tccataagcc gctcttatga ttaaaaaaac gcgatgagat aaaaatgtgt cattttgcga
     6901 caaaattacg tgcttgtgaa agttgttata aatcaaataa gtggttgtga aatttgcact
     6961 ctgaaaagga catcttatct ttaaataagt ggtagcgaat cgctacggaa tagagataac
     7021 acgaggagtg gttagaaatg gctaaagttc tggtgcttta ttattccatg tacggacata
     7081 ttgaaacgat ggcacgcgca gtcgctgagg gtgcaagcaa agtggatggt gcagaagttg
     7141 tcgttaagcg tgtaccggaa accatgccgc cgcaattatt tgaaaaagca ggcggtaaaa
     7201 cgcaaactgc accggttgca accccgcaag aactggccga ttacgacgcc attatttttg
     7261 gtacacctac ccgctttggc aacatgtccg gtcaaatgcg taccttcctc gaccagacgg
     7321 gcggcctgtg ggcttccggc gcactatacg gaaaactggc gagcgtcttt agttccaccg
     7381 gtactggcgg cggtcaggaa caaaccatca catccacctg gacgaccctt gcgcatcacg
     7441 gcatggtgat tgtccccatt ggctacgcag cgcaggaatt atttgacgtt tcacaggttc
     7501 gcggcggtac gccgtacggc gcaaccacca tcgcaggcgg tgacggctca cgccagccaa
     7561 gccaggaaga actgtctatt gctcgttatc aaggggaata tgtcgcaggt ctggcagtta
     7621 aacttaacgg ctaatcttca acaggaggat acgcatgcca actcaagaag cgaaagctca
     7681 tcacgtcggt gaatgggcat ctctgcgcaa tacgtcgccg gaaatagccg aggccatttt
     7741 cgaagtcgcc gggtacgacg aaaaaatggc agaaaagatt tgggaagaag gtagtgatga
     7801 agtgttagtc aaagcctttg ctaagaccga taaagattcg cttttttggg gcgaacagac
     7861 catcgaacgt aaaaacgttt aaccagcgac tcccccgctt ctcgcggggg agttttctgt
     7921 tatttcaccg cctcattcaa cacgctatca aacttatcca tcgggcagaa accgttggca
     7981 tctatcgggc aaccgcttaa ttccagcgtc acgcgctgcg caggtgcctg cagggttaac
     8041 gcatcggcat tacgtaactg ttccgcactc tggtacacat attcaatttt catcaaatcg
     8101 cgattggctt tgctgtcatg ccaacgctgg aaaacgattt tgccgccaat cggcgtgcgt
     8161 tcgttctggt catgcaactg atacggtttg aaatccagcg ccgttaacag agaggctatg
     8221 ttggagtcgt gcccaactaa caccgtgatt ttcggagcac tggcgcgatc ggtgaccagg
     8281 gctttgtcga tataactgac cagcggtttc gcaacattgc gcgccacttc cggtgaggta
     8341 aacagactgt cctggtaacc gtttttcagc ttcgacaata ccttccactg ctggtcagat
     8401 ttgatttctc cccaggccac ctgatccatc ggaaaacctt cgtagtattg caaagtaaac
     8461 gcatctacca gcgagttgcc gactttcagc ggcccggaaa cacctggttc ttgttgatac
     8521 ttggcgctaa aggtattttt gccatccacc agcgaacact gttgtttctc tttacaggca
     8581 ggggaatctt tatagttaac gattttttcc agtagctggt agctgtcggt aagctggagt
     8641 ttgctgagct ctttctccat tgccgccacc gcctgttcac tgaatgcggc ggaatcatcg
     8701 gtgatcatcg ggttaaaggt tgggtccata gtgcccattt tttcctggtg atgcacagga
     8761 atatcacacc cagggaatgc accagtaata aagaactggg cggtagcgac ggtacgttgc
     8821 agactattgg cataggcata aacggtgtcc ggcggcgggc attcccccga tttcactatc
     8881 ccctgctctg ccagccattc acgcatgtaa tggcccatat acacttcgag cacgccacct
     8941 ttggtggtga gttgcccacc ggggacgtcc cattcgggcc atttattcgg cgtcgactgc
     9001 tccagcacac tgccattgtt cgccagcggc gcacgtaagt tatggcggct catcatgagc
     9061 acttgctgta gctgatagcc ttccggtacg gtttgtgcct gagcatttga agcgagtaaa
     9121 actatccctg ccacagttgc ggcgattagc gttttgttca ttcctggcac ctcttttgtt
     9181 atcaatcatt aaagagtgtg acagaaatat gacttttttc cgggaacagg ttcgcaaaat
     9241 ggcttgttga acgcctgttt ttctatatac atcttcaaaa acaggcaagg tttgacgcaa
     9301 actatgcctg attagcccct gcctcgcgta cgccgctgat aattgcacct gtggctaaac
     9361 agaatatctg cggcgttacc aatacagaga caatatttac caataaaaaa gtgtgagact
     9421 aataacaatg gtttttagca tatgcctttg ccattaagtt gcataacgat ttaaatagtc
     9481 aagttgcgca ttcatttcct tattttttaa cttataagcc tgagaaaaaa gttcaggagg
     9541 gaggtaaaca tcataatgat tgccagtgtt tattaaataa ccatactcaa gatcttcatc
     9601 tgttgaaaag gattcatttc ccaaggagat tgatgccaca ttactattaa caataaataa
     9661 tgagcaaatg ctatctgaaa gaacagcaag aaaattcttc accgctttaa tcctatccga
     9721 cgtgtcggta tctaaagcaa gcaaattctt gttatcatta aatataaact cgggtaaatg
     9781 cagtatgcat tttttgtcat tcaagcaact atcaatgaat tgttttttaa aaaacagaaa
     9841 gctccatgac ataaaactac ccacaccctg aattttgttg tcagattgca gatcaacgcc
     9901 aaataatgtc tttacatctt ccctgagatt atagaatgcc gtatcaacaa gagcctcgat
     9961 cgtttgttta tcaacaattt taataaactc ttcgtatact tgcttttgcg tcttatggct
    10021 gcccagcgcc catgaatact catgttcttt taaaaccagt atggctctta acagacaata
    10081 tccatcgcca tctactggag cgtattttaa tccatatgtt ttattaattt cactctgaat
    10141 aaaagatatg ccagacaaat caataccagt atctaaatca gatattctcg gcatatttat
    10201 ctgttctggc tggatactca tattttcatc atctaaacaa tactcagaaa aaacatcatc
    10261 acacgcatta atgttttcga tatattccat aaagccatac caataataac ctttaaaatc
    10321 tgtgatagac tctgaacctc tgcccggcaa ggacactcta acaatatcaa catcttgaac
    10381 atattgtata gtatatgtaa tacctcctga aacccctgaa taaatttgtg tatcattagt
    10441 acgcaatgtt ctcataaata atgatagatc attatcattc tgcatttcct caaattcttt
    10501 ttctagtgat tcacattcca gacatggagt atcctcttca tttactttac gtctgagttc
    10561 attgtacaac cgcataaaac acaccagtgt gttttcaaaa agcgtgattg aatatgtttg
    10621 atttttttcg tactcatttt ttatatcgtt ccaggcctgt ttcaaataat tatttgcagt
    10681 gcaactcatt ccatgtatat tactacccat atatagcgtc tataaaattt aataaataat
    10741 gacgccctag ttaaacttaa agtgcctggt tcaactatca aaaatcgctc accctttttc
    10801 acctgtttaa aatatgttca gcaacccatc ttgatggcga cctcctctcc gcgatgattt
    10861 caataacata ttctgtgttg gcatatgaaa ttttgaggat taccctacac ttataggagt
    10921 taccttacag gggttccttc aatttgtgtt gatttacgcg agataacgct atggaattaa
    10981 aggattatta cgccatcatg ggcgtgaaac cgacggacga tctcaagaca atcaagaccg
    11041 cctatcgtcg actggcccgt aaataccatc ctgatgtcag caaagaaccg gatgccgaag
    11101 cccgcttcaa agaggtcgct gaagcctggg aagtgttaag tgatgaacaa cgtcgcgctg
    11161 agtatgatca gatgtggcaa catcgcaacg atccgcaatt taaccgtcag ttccatcatg
    11221 gcgacggtca gagttttaat gccgaagatt ttgacgatat cttctcgtca attttcggtc
    11281 agcatgcccg ccagagccgt caacgccccg ccgcacgcgg ccacgatatt gaaatcgaag
    11341 tggcggtatt cttcgaagaa acgcttactg agcataagcg taccatcagc tataacctgc
    11401 cggtttataa cgcctttggc atgatcgaac aggaaatccc gaaaacgctg aatgtgaaga
    11461 tcccggcagg cgtcggcaat ggtcaacgca tccgcctgaa aggccagggg acgccgggcg
    11521 aaaacggcgg tccaaatggc gatttgtggc tggtgattca tattgcgcca catccgctgt
    11581 tcgatattgt cggccaggat ctggaaattg tggtgccggt tagcccgtgg gaagcggcgc
    11641 tgggtactaa agtcaccgtt ccaacactga aagaaagcat tttgctgact atcccgccag
    11701 gcagccaggc cgggcaacga ttgcgcgtta aaggcaaagg tctggtgagc aaaaaacaga
    11761 ccggcgatct gtatgcggta ctgaaaatcg tgatgccgcc gaaaccggat gaaaacactg
    11821 ccgcgctgtg gcagcaactg gcagacgccc agtcgtcttt tgatccacgt aaagattggg
    11881 ggaaagcata atggctaatg ttacggtgac ttttactatt accgaatttt gcctgcatac
    11941 cggcatctct gaagaggagt tgaatgaaat tgtcggtttg ggggtggttg aaccgcgtga
    12001 gatccaggaa acaacctggg tatttgacga ccatgccgcc attgtggtgc aacgcgcggt
    12061 acgcctgcgt catgaactgg ctctggactg gccggggatc gcggtggcgc tgacgttaat
    12121 ggatgatatt gcgcacctga agcaggaaaa ccgcctgcta cgccagcggc tttcccggtt
    12181 tgtagctcat ccgtgatgtg tgtttgccgg atgtgatgac gcatccggca actgcgccat
    12241 aatgcctgat gcgacgctaa acgcgtcttt tcaggcgcat aaacgacgtt atctgttttg
    12301 gtggtcgcac tccactaaca ccagcaataa ctggcttagt gccgcgtaaa aaccaaagct
    12361 gtcatactgt cgacaacgcg cagcaaactc tggtaaccat tgccgtagcg ccgtcagtat
    12421 tttttgccgc aaactgtcga ttcttcgcgc aggaacggtc ccctctccca gcgaaaaatg
    12481 cagataactc aacaactcga gatagatcgc cagatgatct gccggttcgt tgaaattgcc
    12541 actggtttcc atccctgcct caactaacaa gcgtttaatc tcttgctcgt cctgtttgta
    12601 ggccgatgca tacggcagcg ccgcttgttt gtcggtcatc agaaacaggc cgcaaaagtc
    12661 agcggccagt tccagacggg catcgtcacg tactgtcagc gtggcaggta atgctgccaa
    12721 cttactgatt tagtgtatga tggtgatttt aaggt
//
LOCUS       DAJTNM010000110        12660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTNM010000110 DAJTNM010000000
VERSION     DAJTNM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.110"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="OO172_003197"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="OO172_003197"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5513281.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1542..2348
                     /gene="tcdA"
                     /locus_tag="OO172_003198"
     CDS             1542..2348
                     /gene="tcdA"
                     /locus_tag="OO172_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5513282.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2399..2842)
                     /gene="csdE"
                     /locus_tag="OO172_003199"
     CDS             complement(2399..2842)
                     /gene="csdE"
                     /locus_tag="OO172_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5513283.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2842..4047)
                     /gene="csdA"
                     /locus_tag="OO172_003200"
     CDS             complement(2842..4047)
                     /gene="csdA"
                     /locus_tag="OO172_003200"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5513284.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4239..4466
                     /locus_tag="OO172_003201"
     CDS             4239..4466
                     /locus_tag="OO172_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5513285.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4817..5734
                     /gene="gcvA"
                     /locus_tag="OO172_003202"
     CDS             4817..5734
                     /gene="gcvA"
                     /locus_tag="OO172_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5513286.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5753..6148
                     /locus_tag="OO172_003203"
     CDS             5753..6148
                     /locus_tag="OO172_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5513287.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6141..7241
                     /gene="rlmM"
                     /locus_tag="OO172_003204"
     CDS             6141..7241
                     /gene="rlmM"
                     /locus_tag="OO172_003204"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5513288.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7285..8016)
                     /gene="fucR"
                     /locus_tag="OO172_003205"
     CDS             complement(7285..8016)
                     /gene="fucR"
                     /locus_tag="OO172_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5513289.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSSALFHIRLKSHYAHKADIAREALAWIEERMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(8074..8496)
                     /gene="fucU"
                     /locus_tag="OO172_003206"
     CDS             complement(8074..8496)
                     /gene="fucU"
                     /locus_tag="OO172_003206"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5513290.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8498..9917)
                     /gene="fucK"
                     /locus_tag="OO172_003207"
                     /pseudo
     CDS             complement(8498..9917)
                     /gene="fucK"
                     /locus_tag="OO172_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10026..11801)
                     /gene="fucI"
                     /locus_tag="OO172_003208"
     CDS             complement(10026..11801)
                     /gene="fucI"
                     /locus_tag="OO172_003208"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5513291.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11834..>12660)
                     /gene="fucP"
                     /locus_tag="OO172_003209"
     CDS             complement(11834..>12660)
                     /gene="fucP"
                     /locus_tag="OO172_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5513292.1"
                     /translation="GAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSV
                     QTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLA
                     QFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAP
                     HKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKY
                     GSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3018 a         3152 c         3369 g         3121 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttagcat gattttgaac aaaaacaaac cactaaagct tattttttgt gcgaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggcggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaac gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgccgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 ccagggcgag ttccagtatc ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgccg gggcattggg cgataaatac atcctcgctt acagcaactc
      721 tctgatggat aacttcatta tggatgtgca gggtagtggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgctg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcattggggc aaaacacaca gtgaagccga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ttggatgtcg ggggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac gggtttgggt
     1261 gctgaaaacg gctccgggcg caggtaacgt ctttagcggc tgatgtgata ttctgtatgc
     1321 cgtttttacg gatatgacag ggtgaggggt aacctcacat cctgattatt gactaagtgc
     1381 gcgttgttca taccggatgc ggcgtgaacg ccttatccgg cctacaaatc atgcaaatcc
     1441 aaaatattgc aggagttatg taggcctgat aagcgtagcg catcaggcat ttatgcgttt
     1501 agggtgaggg ataacctcgc cctttttaat tctgaggtgc tatgtctgtg gtaattagtg
     1561 atgcatggcg tcagcgtttt ggtggcacag cgcgtctgta tggtgaaaaa gcgttgcaac
     1621 tgtttgctga cgcgcatatt tgtgtggttg gtatcggtgg tgtcggttcc tgggcggcgg
     1681 aagcgctggc gcgcacgggg attggcgcaa tcacgcttat cgatatggat gatgtgtgcg
     1741 tcaccaatac caatcggcaa attcacgctc tgcgcgataa cgtcgggctg gcaaaagcgg
     1801 aagtcatggc ggagcgcatt cgccagatta acccggagtg ccgtgtaacg gtggtggatg
     1861 atttcgtgac gccggataac gtagcgcaat atatgagtgc cggttattcg tacgtgattg
     1921 atgccattga tagtgtacgg cccaaagcgg cgctgattgc ttattgtcgg cgcaataaaa
     1981 tcccgctggt cacgaccggt ggcgcgggtg ggcagattga cccgacgcag attcaggtta
     2041 ccgatctggc gaaaacgatt caggatccgc tggcggcgaa gttgcgcgag cgcctgaaaa
     2101 gcgattttgg cgtagtgaaa aacagtaaag gtaagctcgg cgtggattgc gtgttttcta
     2161 ctgaagcgct ggtgtacccg cagtcagacg gtacggtgtg tgcgatgaaa gccacggcag
     2221 aagggccgaa gcggatggat tgtgcatctg gatttggcgc ggcaacgatg gtgaccgcca
     2281 cctttgggtt tgttgcggtt tctcatgcgc tgaagaagat gatggcgaaa gcggcgcgtc
     2341 agggttaaac aatcaggtcg gatgagacgc gaacgtctta tccgacccgg ttctgtgctt
     2401 aaacctgctt cgtcgcagcg ataatcgcct cgcttaacgc atttaacccc tggctgcgtg
     2461 aggcgctaag ctgcgcacgt aatcccagct catcaaacaa tgccagcggc gactgtgcct
     2521 gtaattcggc ggcggttttc ccctcaacgg cagtcaacaa caccgccaac aggccgcgca
     2581 caatgcgccc ttcgctgtcg ccaaagaagt gcattttgcc gttttcagcc acggtatatc
     2641 ccagccagac gcggttttcg catccggcaa tctctttagc ctgcgctttt aactcgtctg
     2701 gcaaagccgg aagctgtttc cccagcatga tcaactggcg atatttatct tcccattgcg
     2761 acaacggtgc gaaggtattg cgtaacgttt ctgcggttac ggttgtgccg aacggatgtc
     2821 cggcgaattg cgggtttgtc attaatccac caataattcc agcgcgcggt caacggcatt
     2881 caccagcgca tccacatcac tctttgtatt atatggcgca aatgaggcgc gcagtgtgcc
     2941 ggttacgcct aattctgcca gtagcggctg agcgcaatgt tgcccggccc gcaaggcaat
     3001 accgtactcc gccagtagcg tcaccatatc gctgtgatga acgccagcaa aatcaaaggc
     3061 cagcaggctg gaatcctggc agcggaatga acgaaagcct ggacgtttcg ccagcgcttc
     3121 ttctgcaagc gttgctaagc tacggctcca gctttcggcc tggttgatat cgtaatctgc
     3181 cagccattcc agcgccgcgc ttaatcctat gacgccagcg acatttggcg ttcctgcttc
     3241 cagtttccac ggcgcagatt gagtggtgaa accgtcaaaa ctcacttcgt gaatcatttt
     3301 gccgccaccc agccagggcg acatcgcttc cagcagttct gatttgccat acagcacgcc
     3361 gatacccgtc gggccataca gtttgtgacc agagaaagca tagaaatcga tatccagttg
     3421 ctgaacatcc gcggggaaat gcaccgcccc ctgagcacca tcaaccatca ccaccatccc
     3481 ggctgaatga gcaaaggtaa tcgctcgcgc cagatccggg caaccgccag tcacgttcga
     3541 catctgaccc aacgccagaa tccgactacg gggagtaatc agttctggca acaaatcgac
     3601 atccggcagt cgctgcgcat taagcggcaa tttcaccact ttggctctag tttgttgggc
     3661 gaccatcagc caggggacga ggttagcgtg gtgttctgcc acgctgacaa taatctcatc
     3721 gcccggttgc agacgcggac gcgcatagca ttgtgccacc atgttgatgg attcagtggt
     3781 gccgcgcgtc cagacgatag ttttatcatc cggtgcattc agtaattgcg ccactttctc
     3841 tcgtgcagct tcataacgcg cggtcaggcg ttgggcttcg gcaaactggc tgcgatggac
     3901 gtttccggcg ctcaggctgt aaaactgtcg agtggcttca accacggctt caggtttaag
     3961 cgcggtcgcg gcgctgtcga gatagacgcc cgcatcctgt agtgcgggaa actgggcgcg
     4021 aaactgcgcg ggattaaaaa cgttcatggt actcctcggc ttgatgcacc gatcgtgacg
     4081 caaaattcac tttgatacaa ggagagtgct aaccatcaga attatctaga tatcctggcg
     4141 aagccgcgta agtaacatat gctgaatagt gttaggcgtt aattttagcc tgttaatcaa
     4201 tactattaag atttattaat cgctttaagg agaagatgat gaaaaagact gccgcaatta
     4261 tttctgcctg tatgctgact tttgccctga gcgcctgttc cggttcgaac tatgtgatgc
     4321 acaccaatga cggacgtacc atcgtctctg acggcaaacc acagactgat aacgataccg
     4381 gtatgatttc gtataaagac gctaatggca acaaacagca gatcaaccgt actgacgtga
     4441 aagagatggt cgaactggaa cagtaattcg cgatcgcaag gtaaaaaaaa gcaccgcaat
     4501 taggcggtgc tacattaatc actatggaca gacagggtaa atgtacagga agtgaaaaaa
     4561 ggtagctttg ctaccatggt ctgaatcgca gaccaattgc aaacacaaca acacaacatc
     4621 acaaccgtaa gccaaaagtt caccagaata cgcattccga taaaactttt cgttccggct
     4681 caggaagtgc cgccactata ggtatttgct ggtagaagct caacggacaa tttataatgg
     4741 ctcagattaa aaaaactaat aggttacaca gtgtgatcta attgttaaat tcatttaaca
     4801 tcaaagttta aaagccatgt ctaaacgatt accaccgcta aatgccttac gagtttttga
     4861 tgccgcagca cgccatttaa gttttactcg cgcagcagaa gagctttttg tgacccaggc
     4921 cgcagtaagt catcaaatca agtctcttga ggattttttg gggctaaaac tgttccgccg
     4981 ccgtaatcgt tcactcctgc tgaccgagga agggcaaagc tatttcctcg atatcaaaga
     5041 gatattttcg caattaaccg aagcgacgcg taaactccag gcccgtagcg ccaagggggc
     5101 gttgacggtc agtttactcc ccagtttcgc cattcattgg ttggttccgc gactttccag
     5161 ctttaattca gcttatccgg gaattgacgt tcgaatccag gcggttgatc gtcaggaaga
     5221 taagctggcg gatgatgttg atgtggcgat attttatggt cggggcaact ggccggggct
     5281 acgggtggaa aaactgtacg ccgaatattt attgccggtg tgttcgccgc tactgctgac
     5341 aggcgaaaaa cccttgaaga ccccggaaga tctggctaaa catacgttat tacatgatgc
     5401 gtcacgccgt gactggcaga catatacccg acagttgggg ttaaatcata tcaacgttca
     5461 gcaagggcca atttttagtc atagcgccat ggtgctgcaa gcggctattc acgggcaggg
     5521 agtggcgctg gcaaataacg tgatggcgca atctgaaatc gaggccggac gtcttgtttg
     5581 cccgtttaat gatgttctgg tcagtaaaaa tgctttttat ctggtttgtc atgacagcca
     5641 ggcagaactg ggtaaaatag ccgcctttcg ccaatggatc ctggcgaaag ccgctgctga
     5701 acaagaaaaa ttccgctttc gttatgaaca ataatttacg tagggtacga ccatgaccag
     5761 ccgttttatg ctgattttcg ccgccattag cggcttcatt tttgtggcac tgggcgcttt
     5821 tggtgcgcat gtgttaagta aaaccatggg ggccgttgag atgggctgga tccaaaccgg
     5881 tctcgaatac caggcgtttc atacgctggc gatcttaggt ctggcggtgg caatgcagcg
     5941 tcgcatcagt atctggtttt actggagtag cgttttcctc gcgttaggca cggtgctgtt
     6001 cagcggcagc ctttattgcc tggcgctgtc ccatctgcgt ttgtgggcgt ttgtcacacc
     6061 ggttggcggc gtgagcttcc ttgcgggctg ggcgttaatg ttagttggtg ctatccgttt
     6121 aaagcgcaag ggcgtaagtc atgaataagg ttgtattgct gtgccgtccg ggctttgaaa
     6181 aagagtgcgc cgcagaaatt accgataaag ccggccagcg ggaaattttc ggttttgccc
     6241 gcgtgaaaga gaatgcgggt tatgtcattt atgaatgtta tcaacctgat gatggcgata
     6301 agttaatccg tgagctgccg ttcagttcat taatttttgc ccgccagtgg tttgtggtgg
     6361 gggaactcct gcagcatttg ccgccagaag atcgtattac ccccattgtc ggcatgttac
     6421 agggtgtagt agagaagggc ggtgaactgc gtgttgaagt tgccgatacc aacgaaagca
     6481 aagagttgct gaaattctgc cgtaagttca ctgttccgct acgcgctgcc ttgcgcgatg
     6541 ctggggtgct ggcgaactat gaaacgccga agcgtccggt tgtgcatgta ttctttattg
     6601 caccaggatg ctgctatacc ggttactcat acagcaacaa taattcgccg ttctatatgg
     6661 gcattccgcg cctgaaattt ccggcagagg cgccgagtcg ttccacgctc aaactggaag
     6721 aggcatttca tgtgtttatt cctgcggatg agtgggatga acgcctggcg aacgggatgt
     6781 gggcggtgga tttaggcgct tgccctggcg gctggaccta ccaactggtg aagcgcaaca
     6841 tgtgggttta ttccgtcgac aacggcccga tggcgcaaag tctgatggat accgggcagg
     6901 tgacgtggct gcgggaagac ggtttcaaat tccgtccgac acgcagcaat atctcctgga
     6961 tggtatgcga tatggttgaa aaaccggcta aagttgcggc attgatggcg cagtggctgg
     7021 ttaatggctg gtgccgtgaa accattttca atctcaaact gccgatgaaa aaacgctacg
     7081 aagaagtatc acacaatctg gcgtatattc aggcacagct tgatgaacat ggcataaatg
     7141 ctcagattca ggcgcggcag ttgtatcacg atcgcgaaga agtgacggtg cacgtccgcc
     7201 gcatctgggc tgcggtgggt ggtcgtcgcg acgagcgata acaggcaagg ccggggcgtc
     7261 gtctccggcc tgctaccctt tccgtcaggc tgttaccaaa gaagttgcaa cctggcgctc
     7321 atcagaaata atgtgcgtta cctcatccag atacccgatg cgggcttccc ctgaacgatt
     7381 aaatttactt ttatcaatca ataacaacga ttgcgcggca cgttttaata gcatcgattt
     7441 gtaatcagcg ttgatcgcat tggagtccca cagtgcgccg ctgctatcga tcccttcaca
     7501 agaaaaaata aacagatcga tttccagcga tttaagttgg gaaatcagcg agggattgac
     7561 gtaacagcca tatttgcgct caagtgtgcc gccggaactg atcagttgaa tgcgttcgcg
     7621 tttaccgagt tcatggcaaa tcggatggct attggtgaag acctgaatgt tgatgtcagg
     7681 caactggcgt gccagatacc agcaagttga actggcgtct aaggctatca ccatcctttc
     7741 ttcaatccac gcgagcgcct cgcgcgcgat atctgcttta tgcgcataat ggcttttcag
     7801 cctgatgtga aagagagcgc tactgtcttg attttgacgg tggatatatt tagcgcgtcc
     7861 atgattgcgc agaattttac cctgcgtctg taattcattg agatcgcgac gaatggtttc
     7921 tttactgacc tttagctgtt cagagagagc ttccgtggtc aggctggtat ggttcagcag
     7981 caggtcgact atcgcttgct ggcgtgccgc tttcatattt ttcaccccat cattcgataa
     8041 ccgcccgcgt catgcgggcg taccggtatg agattacggt gttacccctt tttttaaaag
     8101 aatattcccg tacttcgctc gttcgcctgt gataacgatc gcaaaggctt tttgcgcccg
     8161 ttcataaaac gcaaaacgat tgatgcggat gatgtcagga cacggggctt gtagtgaaag
     8221 cgcattacgg taacgtcgtt ctacttcagg atcgagagtg tcaccctcta ccgccgccat
     8281 catcaccagc ggcggggcat aactgtccag ctcaaataac gggataatcg cctggagcaa
     8341 gtcgctcacc aacaggccat cagcgcggat cacctgcggt cccatcgaat gggcgggaaa
     8401 gtgagcatcg gaaaaaataa tttcatctcc atgtcccatc tctgccagca ctttcaatag
     8461 ttcgggagaa attaacggcg aaattgtttt cagcatttca cacttcctct ataaattcag
     8521 gttcagtttg cgggtagaaa taacggtact gataatgaat ctgtgcacgg gcttcttccg
     8581 ggctgttaaa ttcccctacg ccataccaac cgaacagcgc agctcctgcg acggtcgttt
     8641 cggcatcgtc gagaactttt accggaatat caagcatatt ggctttaatc tgattccaca
     8701 atgtgttgcg acttcctcca ccgactaaca ataattcata ggctttaaag tgcccgattt
     8761 tttccagcat ctgtagattg cgctgtaatt gcgcagttaa cccttccagc gccgcgcgat
     8821 agaaatgccc ccgcgtggta ttaagcgtca ctccttgcca gccagcgttc tgacacgaca
     8881 ataaatcaca ctgcattttc acaccatccg cgccaggtgc gatcagacga gcttcttcaa
     8941 tcaacatttg ccagggtgtt tcagccgtcc agaacagttt tctcacccat tccagcacgc
     9001 cggatgccag ccattgcata cctgggttat acaaccatgc ctggctatct agttcgcagg
     9061 tggaaccggc gtactgactt aacagcgaag tatcaaccta ggcgctgcga accattaaaa
     9121 tttcccatgt accggaggag agcacgggtt cattttgttc ggcaccagcg ccaaaaaggg
     9181 cgaactgggt atcgtgacct gcggaaatca ccggtatgcc aacgggtaag ccgagcattg
     9241 ctgcggcgct gttctgtagc gtaccaatct gttcacccgc ttccaccaga cgagggaaga
     9301 gtcggcgtgg aataccggtg gcttgtaaaa tttgcggact gaaatcgcgt tgctggatat
     9361 ccagcatctg gctggttccg gctatcgtga tatcagtagt gaattcgccg gttaaacggt
     9421 ggttaatcag cgacgaaatg aagagccagg cgtgcgcgcg ttccagcagt tgtggatgat
     9481 tttctttcaa ccacaccaac ttatataacg tattgaaact aaaggctccg acgccagaaa
     9541 tagcctgcaa ccgctgtgcg gagattaacc gttcaatatt gtccataacc gctgctgttc
     9601 gcggacattt ccagctaatg atcggataga gcagattgcc ttgcttatct accagagcgc
     9661 catccacacc aaaggtggtg acggcgatac cgcggatgtg gcattcagtc agttcactat
     9721 tgatttgccg acagcaatca gcaaagcgtt gcaaaatggc agtctaaaga ccactggtgc
     9781 caggtgttgt tttccatcgc gatatcgctg gcattaggcg ttgaggcgcg ggcaacaatt
     9841 tttccctgcc gattaaccgc gatggccctg acattggtcg cgccacagtc gagtaccagg
     9901 ataacttctt gtttcataat cgctcctgca atatagccgg ataacattgt ttatccggct
     9961 aaccactctt gcttgttcag gcccggtagc cggagcgacc gggcatcaca tcagggtgta
    10021 atgtattaac gcttgtacaa cggaccgtag ttctggcaag cgcggtaatc ctggccttca
    10081 atatccatgc cgtgcgcagc ccaggcagaa ggacgataca ctttggtctc ttcaacgttg
    10141 tgcatacata ccgggatacg cagcatggag gcaagggtga taaagtctgc gccaacgtgg
    10201 ccgatggtca gaactccgtg gttggcaccc cagttcgcca ttaccgaata cacatccgta
    10261 aacggccctt taccagtgag gcgcggtgca aaccaggtgg ttggccaggt tgagttggtg
    10321 cgtttgttga ggatgtcatg cacatccttc ggcaattcca cgctccagcc ttccgcgatt
    10381 tgcagtaccg gtcccaggcc tttgatgatg ttgacacgag tcatggtgaa cgggacgccg
    10441 ccttcggtaa ggaagcggga agagtaaccg ccgccacgga agtattcgtg gatcgccggg
    10501 caccattcgg tagcggcgag gcaagcgtca gcctcttgct gagagatttc ccagtgcggc
    10561 ttcatcgtcg ggttaccttc gctgtcgcgt tgtttacagg aaccgtccag cgcagcagaa
    10621 cctgagttga tcaaatggat gatgccgtgt tctgccagtc cgtccagttt atgccccgtt
    10681 acacgctcaa ttgcttctgg tgaccagtag gtacgcacat cggcaaatac ctgagcggtg
    10741 ccggtgagct ggtgacccat tagcattgcc acgccgttaa gactgtcgtt ttcggtcgcc
    10801 acgacaaagg gttcgcgcac gccattccag tcaaatgaac tgttgaggat cgcttcggcg
    10861 gtgtcaccat tgggatattg atcggtccag tgacgttgcc cctggaagcc cgcagcgatg
    10921 gcgttgtagc caagtgattc ttccacgcga ccaatatcgg ccagtttgct gttgccttgc
    10981 atcatgtcgc ggatacacat cgccatcagt aaactttcgc gcagaactgc gcggctttgc
    11041 tcggcattgc gttgatactg tttgttattt tcatcttcgc catagcggaa gtttttatca
    11101 gcccaggcca gtgccatttc cagttcggct tcgtcgtaaa tcttctgatc gatacggcga
    11161 cgcagttcgg tcatatccac cgcctggact ttcattccca gccaggattc aaagaagttg
    11221 tgatcaacaa tagaaccggc gatacccatc gaaacgccgc ccagcgacag atagctttta
    11281 cctttcatgc tggcgacggc caaaccggcg cgggcaaagc gcagcagttt ttcttcaaca
    11341 tcggcaggaa tcgatgtgtc atcggcatcc tgaacgtcat gaccgtaaat ggagaatgcc
    11401 gggatgcctt tctggctgtg agctgccaga gccgctgcca ggtaaacagc gccggggcgt
    11461 tcagtgccgt taaagcccca aatggctttc gggcgggttg gatccatgtc gatggtttca
    11521 ctgccatagc accagcaagg cgttaccgta atggtgaggc ctacattctg gctgctgaat
    11581 ttttcttcgc aagcagcggc ttcagccata cccgcgatac aggtatcgga aatgacacac
    11641 tcgacggcag ctccgcaggt atggcgcagt ttctcggtca gcagtgcggc cgtagctttc
    11701 gccatattca ttgtttgttc ttcaagcgac tcacgaacac ccatgcgacg accgtcaata
    11761 accgggcgga taccaatttt cggtaagctg atttttttca ttacagattc ctcactttat
    11821 tcggaaaata tgttcagtta gttgccgttt gagaacggaa acgggcaaag ataaagatga
    11881 ccgcgaagca gagtgcgggg atcagttcag cagtggggat gttgcccgcc gcgtcactga
    11941 caaaacccat gaccggagtg acaataccgc cgccaataat ggtcataacg atgaaggacg
    12001 aaccgtattt ggtgtcctgg ccgagattct taatgcccag cgagaagatt gttgggtact
    12061 gaatcgacat aaaggcgctg cataaagtca gggctattaa gcccacatga ccgccagcga
    12121 aggctgagat caggcacagt gccatagcga ttaatgcgta ggcggccagg actttgtgtg
    12181 gtgcgaagcg actgatgagc caggtaccgg tgatacgacc aataaagaag cacaccatgg
    12241 ttccggttaa atagttagcg gcaaagcctg cagtcatacc tggaatttct tctacagcgt
    12301 agcgaatcaa atagctccag caggccgttt gtgcgccgac atagcagaat tgcgctaata
    12361 ccgcccagcg ccagtgacga atacgcgcca gacgagaaag cgatgcggag aacgatcctt
    12421 gtttggcgtc actgtgatta tcactctgca atgccgggaa tttcgtcagc atgatcagca
    12481 gggcgaccag taacacgata gccacgatga tcatataagg tgtctgtacc gataatacta
    12541 ggctgtgttt atacgcactc aattgctctg gagacatttt atcgagaacg tcttgcgatt
    12601 gatgtggcac gttagacaaa ataagacttt gcccaaagac aaccgcgata attgcgccaa
//
LOCUS       DAJTNM010000111        12657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTNM010000111 DAJTNM010000000
VERSION     DAJTNM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.111"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..212
                     /gene="yfdR"
                     /locus_tag="OO172_003210"
     CDS             <1..212
                     /gene="yfdR"
                     /locus_tag="OO172_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5513293.1"
                     /translation="VMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVI
                     PLAPGHAYGMFMERFNELSELRKCA"
     gene            203..565
                     /locus_tag="OO172_003211"
     CDS             203..565
                     /locus_tag="OO172_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513294.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            565..870
                     /locus_tag="OO172_003212"
     CDS             565..870
                     /locus_tag="OO172_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513295.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGNNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            1097..2260
                     /locus_tag="OO172_003213"
     CDS             1097..2260
                     /locus_tag="OO172_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5513296.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVVTKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKESTNDV"
     gene            2610..2972
                     /locus_tag="OO172_003214"
     CDS             2610..2972
                     /locus_tag="OO172_003214"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCR5513297.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            2969..3886
                     /locus_tag="OO172_003215"
     CDS             2969..3886
                     /locus_tag="OO172_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5513298.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            3899..5164
                     /locus_tag="OO172_003216"
     CDS             3899..5164
                     /locus_tag="OO172_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR5513299.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(5231..5854)
                     /locus_tag="OO172_003217"
     CDS             complement(5231..5854)
                     /locus_tag="OO172_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5513300.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(6605..6943)
                     /locus_tag="OO172_003218"
                     /pseudo
     CDS             complement(6605..6943)
                     /locus_tag="OO172_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            7065..7325
                     /locus_tag="OO172_003219"
     CDS             7065..7325
                     /locus_tag="OO172_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCR5513301.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(7343..10078)
                     /locus_tag="OO172_003220"
                     /pseudo
     CDS             complement(7343..10078)
                     /locus_tag="OO172_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(10079..11410)
                     /locus_tag="OO172_003221"
     CDS             complement(10079..11410)
                     /locus_tag="OO172_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5513302.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPNVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPQDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMLTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            complement(11420..12112)
                     /locus_tag="OO172_003222"
     CDS             complement(11420..12112)
                     /locus_tag="OO172_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5513303.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(12115..12570)
                     /locus_tag="OO172_003223"
     CDS             complement(12115..12570)
                     /locus_tag="OO172_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5513304.1"
                     /translation="MIQFKPTRNIDLIEAVGNHTDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(12570..>12657)
                     /locus_tag="OO172_003224"
     CDS             complement(12570..>12657)
                     /locus_tag="OO172_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513305.1"
                     /translation="LASNGSTLTIQSNGAAFTATTIKIIAEQ"
BASE COUNT         3361 a         2929 c         2836 g         3531 t
ORIGIN      
        1 cagttatgag tacgcccgtg aaatatgccg atctcatcat gctggcaacc gaacgccgcg
       61 atctcgggct tgatgatggc tctttctggc ctgtactgga aggtatcccg gcaacagaga
      121 tgttcaacgt gattccactg gcaccgggcc atgcctacgg gatgtttatg gaacgcttta
      181 acgagttatc ggagttacgc aaatgcgcat gaatgttttc gaaatggaag ggtttcttcg
      241 tgggagatgt gtaccgcgag atctgaaagt aaatgaaaca gatgctgaat acctggtgcg
      301 taaattcgat gcgcttgaag ctaaatgtgc agcacaggaa aacaaagtaa taccagtgtc
      361 aactgaactg ccaccagcaa atgaaagtgt tttgttattc gatgctaacg gagaaggctg
      421 gctaattggt tggcgttctc tctggtacac ctggggacaa aaagaaaccg gagaatggca
      481 gtggacattt caggtcgggg accttgaaaa cgtcaatatc actcactggg cagtaatgcc
      541 aaaagcaccg gaggctggag cataatgacc acttttaccg acaaagaact gattaaagaa
      601 attaaagagc gtatcagcag ccttgacgtg cgagacgata ttgagcgccg tgcttatgaa
      661 atcgcactcc tatctctgga agtagaacca gatgaacgcg aagcttatga attattcatg
      721 gaaaagcgtt tcggtgactt agtagatcgt cggagagcaa aaaacggcaa taacgaatac
      781 atggcatggg atatgactct cggttggatc gtctggcagc aacgagctgg tatccatttt
      841 tcaacaatgt cacagcaaga ggtgaaataa tggagccata cagcctcaca ctcgatgagg
      901 cctgtcattt tctcaagata tccagaccga ctgccattaa ctggatacgc acagggcgtc
      961 ttcaggcaac acgcaaagat cccactaaga ataaatctcc ttacctcaca acacgacaag
     1021 cctgcattgc ggctcttcag tctccgctgc atactgtcca ggtgagcgcg ggtgatggca
     1081 taacagagga aagaaaatgt cactcttccg cagaggtgaa atatggtacg ccagtttcac
     1141 attgccgaac ggtaaaagat ttaaacagtc tcttggaaca aaggacaaaa ggcaggcgac
     1201 agaactccat gacaagctaa aggctgaagc atggcgggtc agcaaacttg gtgaaatacc
     1261 tgatataacg ttcgaggaag cgtgtgtcag gtggcttgaa gagaaagcac ataaaaaatc
     1321 actggacgat gacaaaagcc ggatcggatt ctggcttcaa catttcgcag gaatgcaact
     1381 aagagacatt actgaatcaa aaatttattc agcaatgcag aaaatgacga accggcgtca
     1441 tgaggaaaac tggaaactca gggcagaagc atgcagaaaa aaagggaaac ctgttccaga
     1501 atacacgcca aaaccagcgt ccgttgtaac gaaggctacg catctttcat ttataaaggc
     1561 cctactaaga gccgcagagc gtgaatggaa aatgctggat aaggcaccaa ttattaaagt
     1621 gcctcaacca aagaataaac ggatccgctg gctggagccc catgaagcac aaaggctgat
     1681 tgatgaatgt ccggagccat taaagtctgt tgttgaattt gcactggcaa caggcttaag
     1741 acgctcgaac atcatcaacc ttgaatggca acaaatagat atgcagcgcc gggtggcatg
     1801 gataaacccg gaagagagta aatcaaaccg cgcaatcggc gttgcgctga atgatactgc
     1861 atgtcgcgtt ttgaaaaaac aaatcggaaa tcatcaccgt tgggtatttg tgtacaagga
     1921 aagctgtacc aaaccagacg gaacgaaagc gccaacagta aggaagatgc ggtatgacgc
     1981 aaacacagcc tggaaagcgg cgctgagacg ggctggtatt gatgatttca gatttcacga
     2041 cttgagacac acctgggcaa gttggctggt tcaagccgga gtcccgttgt cagtgttaca
     2101 ggaaatggga ggctgggagt ctatcgaaat ggttcgtcga tatgctcacc ttgcacctaa
     2161 tcaccttacc gaacacgcac ggcaaataga ctcgatcctg aacccatcgg tcccaaattt
     2221 gtcccagtca aaaaataagg aaagtactaa tgatgtgtaa cttattgatt taaatggtgc
     2281 cgataatagg agtcgaacct acgaccttcg cattacgaat ctgtagcacc aatcataact
     2341 atctgtttta gcaagcatta accgcattca ctaagatata gttgatggca caaacagaaa
     2401 gttgatgaat gatgttgcca tatatatgtc acaaatacgg cacaacgatc ttcaaacata
     2461 tagccacaca ttcacagaag agcacaaagc cttgcaatcc agtgcaaaga tttgtgtgcc
     2521 agagtttttc tcatcaacta ccgcaagtat cgatcgattg agacttggat gatagacttc
     2581 atgcctttga ttattagctg atagaagaaa tgttaaagct atttgcaaag tacacatcta
     2641 taggcgtgct gaacacactt atacactggg tggtttttgg tgtatgtatc tatgccgcgc
     2701 ataccaatca ggctatggca aacttcgcag gtttcgttgt ggctgtgagt tttagcttct
     2761 ttgcgaatgc aaaattcaca ttcaaagcat caactacaac gatgcgctac atgttatatg
     2821 tcgggttcat gggaacactg agtgctactg ttggatgggt tgctgataga tgttcacttc
     2881 ctccaattgt cactcttgtt accttctccg ccatcagctt ggtgtgcggt ttcgtctatt
     2941 caaagttcat tgtctttagg gatgcgaaat gaagatatct cttgtcgttc ctgtcttcaa
     3001 tgaagaagaa gcgataccaa ttttttataa aacggtacgt gaattcgaag agttgaagcc
     3061 atatgaagta gaaattgttt tcataaatga cggaagcaaa gacgctacgg agtcaatcat
     3121 taacgctctg gctgtttctg atccgctagt tgttccgctg tcatttacac gcaactttgg
     3181 taaagaacca gcattgtttg cagggttaga ccatacaacc ggcgatgccg tgattccaat
     3241 tgatgttgac ctgcaagacc cgattgaggt tattcctcat cttattgaaa agtggcaggc
     3301 aggtgctgac atggtgcttg ctaaacgttc agaccgctca actgatggac gactgaaacg
     3361 taagacagct gagtggttct ataaattaca caacaaaata agcaccccaa aaatagagga
     3421 aaatgtcgga gattttcgac tcatgtctcg tgaggttgtg gagaacatta aactattgcc
     3481 tgagcgcaat cttttcatga aaggcatact gagctgggtt ggtggtcaga cagatgtcgt
     3541 tgaatatgta cgcacagagc gtgttgctgg catctcaaaa tttaatggct ggaaattatg
     3601 gaatctggca ctggaaggta tcacaagttt ttcaaccttt cctcttcgcg tatggactta
     3661 tataggcttg tttgttgcaa gcatttcatt tttatatggt gcatggatga ttatagacac
     3721 cattgtcttt ggtaacccag tacgcgggta tccctccatg cttgtatcaa tacttttcct
     3781 gggtggagtg caactgatcg ggattggtgt tctcggtgaa tatataggta gaatttattt
     3841 agaaacaaaa tcaagaccac ggtacttaat taaaagcaga aaataacagg atgatcttat
     3901 gaagatattc ataatgcgca attggcacaa gatatcaatt tttatacttg catttacttt
     3961 gatttggctt aggcgcattg acattttgac aaatgcccag ttttgggctg aggatgcagt
     4021 tttttggtat aaagatgcat acgaacaagg ttttttcagt tcactcataa ccccaagaaa
     4081 tggttatttt caaacagtat caacactcat tgttggtgcg acgacattta ttaatccaat
     4141 ttacgcacca ttattatcaa acttttttgg aataataatt agagctataa ttatatggtt
     4201 cttatttacc gatagattta aattcctaag caccacatca aaaatcttca tctctgtata
     4261 tttaatttgc atgcccgggc ttgatgaagt ccaagccaat ataacaaatg ctcactggta
     4321 tttatcattg tatgttgcaa tgataataat atctgaagaa tccaagtcaa aattatggaa
     4381 agcacatgat ttatttttta tagtattatc tggattgagc ggtccattta ttatattcat
     4441 aattgcatca tctgttttta aatatttaca cttatcaaaa ggagtaataa gcataagagg
     4501 tttatactta ttttatactc gactgccata tttggcaatg atcttgtgtg ggctgataca
     4561 ggcaacatca ataattctta catttaatgg aacaagatca catgctccat taggattcag
     4621 ttttgatgtt atgtcatcta taatatcatc taatgtattt cttttttcat ttgtcccttg
     4681 gaatatcgct aatgctggat gggataatca ccttttatcg tacacactat caactttagt
     4741 aattacatta tgtgttttca tttatattaa aggaaattgg caaatgaaag tgtttgccac
     4801 ccttccaata cttattgtcg cattttctat ggcaaaacct caactggcag attctgttcc
     4861 tcaactgcca acacttgcaa ccggaaacgg gtcaaggtat tttgtaaata ttcatattgc
     4921 aattttttca ttaatatgcg tttgtttgtt tcaatgcata aagaataaaa ccttaaaaat
     4981 attctttaaa atctatgttt cagtcatcct tattgcaatg ataaaattaa acttttttat
     5041 aacccctctt cctgacatga actggtctca aggagcagaa ttaataaata aagcaaaaca
     5101 tggagaagcc gtctcattta cagtgctccc tccatggtta actcttgatt taataaaaaa
     5161 ataaaacttc acattaataa ataacaatga atccgcctcc ctacgtattc ccgtagggag
     5221 gctgtcgtgt ttatagtagc ctggtaatgc taaatgcaaa cgaactggag attcctgctc
     5281 cgctaagcgt attgcgcagg gtcaccctaa atgtatctac accaatataa gtaacatcac
     5341 aactaactga atcaggcgca gatataggtg ttatctgaac cacataacca ttagtagctg
     5401 ctgcgagcgg atgagaaaac gttacatcat acacaccagt agcatgcgcg ttaacggatg
     5461 aaacacccgg cgcaatgtct acactaccat tggcgttaat tctccctgag cgcgtaactg
     5521 actctgcaga aaccgatgtg gccttactgg ttatttccat aaaggtgcaa ccaatatcga
     5581 tcaattcgca attgttccct gttatccagt atggtcgatc tgcacttggc tgataatccc
     5641 ctgcgctctg gaacgttgtg ccattgagta agactataac ttttccccca cctgagcttg
     5701 ttacttgtat attcgtgtgc gtgtagcgtt cactgctcac gcgatgaaaa tttccaccgt
     5761 ttataactac cgttacaggt cgcgtcccag tattatctat agcaaggtct gcccccccgg
     5821 cattagcctc gaagtatggg ttgtttaaca ccaaagcaca cgaaccgtta aggccattta
     5881 tggctaattg cgcaccacct gtaccggaat ccccctgagt accatttcct tcacaagtca
     5941 ggctgttgat agtagttcct gccccccatc tatcaccaag tataccccat cgtgtattct
     6001 ggcttgcagt taatcggtta actgtaatgg cattaagctc tgacccttca ctggagttaa
     6061 gtgcgcgcca gccaatacgg ttaccctgta agtacacatt atctaactct gacgttaaca
     6121 ccgagtctgt acgcaaccct tcgccggtag tgtgcccata agacacaatg ttctctaatt
     6181 tagtgtatgc tttattggtt acgagaagac cgtaagacga tcccgaacct ttaagtgtaa
     6241 tgtcccccag ataatcatag gcgtgtacac gttgggttgc cccgactgta tcaccgagca
     6301 tctttaagtt gaaatccgag gcatggcagt taaatatcgt ttctgccata ttttctccgg
     6361 aataatgagc acggactcct gtcgaaccaa taaatttagc gtcatagttg ccgtaatcaa
     6421 gaacgagtgg tgacgcgata tcgtacaccc cttgctttat ttttaatgtt ccaccgaaag
     6481 gccacgatgt aagcgggtct tgatatattt cattttttat tgcaaatagc tcctcttgga
     6541 gatttttgcc gctattagtg ccaatgtgag cagctccttc atctgattga agtaactgct
     6601 ttaactgatc aggatcgtac ttcagcacat tctgaaaata gaactgctgc gaaccatacg
     6661 catcatatac agccattgaa tggccttgct cagttacaaa tttggcaatc tgcccgttgt
     6721 acacaggata tccggcagca ttgataacaa ttggctgaga aacagggacg tgagatccgt
     6781 cttcattctc tacataaacc tgaatctggt tttcaggatt taccgggtca gtgtcaattt
     6841 ttccgatata aattttgcca ttggctacgg ctttaaaaga acgcgccata gtgaagagtt
     6901 gcgaaggcat gctgaccaca acatttgcgg cgatatctga catttcattg ctccagacga
     6961 atgatatgat gcaatcatga tgtgattgca taccgaaatg gcactattga atatttatcc
     7021 agtaggttac gatgccattc cacccaactg gtgaggcatc aaggatgtac agcaaatacg
     7081 acgaggcaca gtttcacttg agacttccgc atgaactcca cgcgaaaatt aaacagtgtg
     7141 cgaagatgaa taacagttcg ctgaactcag agataattgc agcgattgaa gaatcattgg
     7201 ctaaacaaag ctctgcatca gtttacattg acgatgcaga gcgtatggca gaacaacaat
     7261 ctgatatggt taagaaaatt gtctttgata cgctcaagga gctatataaa aaagacagca
     7321 actaaccatc cgtggctttc cgttacttct ggttatcatc actgttcatc cagtacatga
     7381 tgcctagcct tgatatggtt ttcttatctg attctggaag tgagttgtaa aacgagcgcc
     7441 aagcagaaga gcgtcttaca gttgtatcaa cgatgctttc tggtgcttgt tttactgcca
     7501 gccttgctgc tttctggaat tctggtgagc taattagctt ttctgcagat tcaattgatt
     7561 cagcgccgcc catcgccctg attttgctgg cgacttttgc cccaatttct gacccagcaa
     7621 cagcacctac tgcactgaac ggtcctgaca cagttgaacc aaccgcagtg ccaatgcgtt
     7681 gggcatggtt agcaacaaat tcatttgccg cagtgacgcg attaaaccgt ttgacgaact
     7741 cgtttagtct tcctgtagtt atctcgtaag attttgcatc tttgatagcc tttgcgacct
     7801 tatatacatc gttcagacct gacatagtct cttttggtaa atgtctggca agattgcgca
     7861 tctgcccgtt tgctaacatg ttttgatacc agtcagcaaa ccctgcagga ttaaaatcag
     7921 cgccacgttt tccgttcgaa agcatatccc gaagacctgt tcccagtagc tcggttctta
     7981 gcttcctgga cggcgtgttt tgcatcaatt cacgaaatcc tttggcatcg cctttcgaca
     8041 ttgcctgtag tgctgtagtt gctttgcgag aaacatcacc gttcagcgtt ctgccagtta
     8101 gattaatcat ttgctcttcc atgctttttc gcatctgaac aagcctctga gcaacttcaa
     8161 aatcacgtaa tgcatttgtc tctgacagcg ccgccttttg atcatcggcg agcgaaccgt
     8221 aaagcctcga taaagcagcg cgatcagcat ctttatatgg tccagaattc ttatgaagtg
     8281 catcaccaac aagtcgtcgt tgcttattga gatttgcata agttaatgcg ccgttcttgc
     8341 ctggattaac tgcccgaaag actctctttt caatagggtc aaggtttttc atcccgccaa
     8401 gatcttctgc cacctgtttg agcattgacc ttgttgatgg cgcatcaatt tttgcacctg
     8461 caggcatcgc cttttcaaca ttgcgataaa gctggtcgct tcgtttcatc agcgcagaca
     8521 ttcttccatt gaccgtatca ataaacttgt cgctcatacc aagagcatct tttgcaccgg
     8581 aaacgcgatc aattatctca cccgcgccct gtgctagctg cctgattgct tcattttcct
     8641 gcacctgtag agcagaaccc gcacgggact tgattgcttg ctcaactgcc ttgtattgct
     8701 cgttcccgga aaagtgtgaa gggagtaatg aatcaacatt aagcctttca gcagactcca
     8761 gaacttcagc ctgaggattg atatctagtt catcaagcga tgaagcgaga tttggccttt
     8821 tttgtgctgc tactgtgcga acgacttcct ctgggttctt cgtcgcaggc gtcattggta
     8881 caccttgtgc tactcttgcc tctgcggcat gaaccgcagg atttgttgct gcagcggcat
     8941 caccggagaa tggtgatgtt tctgttggcg cagtggtcgc tctctgtgca gatgttttat
     9001 tgccactaat tatatttgat gcaccacgaa caccacgggt aacaccattt atcaggccat
     9061 gcgtagcaat gctaagtccg gcattaatag cggcattttc ggcaaaatca ccccgttgat
     9121 tcgccgcgtc agcgagagaa ccaatgacca tgtttccagc tacacctgct ccaggaacaa
     9181 gataaccgcc tattgactct ccagcttgcg cgtaggggtc tgtcggtctg tctactggac
     9241 gataaacatc atccaatact tttggccccc caagcccctg actgattgca ttaatcagac
     9301 ttgcgccacc ctgcaatacg tcaaatggta tgtttaccag accacgacca gcctgttctg
     9361 caatttaccc tgcactttga ccaccagtga gccaatcgcc agcttgttgc atcaatgatg
     9421 gttcttcccg tgttggtgca ttattggcct gattaactgt ttgttgctga acaggtttcg
     9481 gtgcgacaga acttacgggt tgaggtggct gctgactggc tgcctgctgc tcaatctgag
     9541 caaatggatt atttgggtct gactgcacac ctgatgccga tacttgttcg gatgactgta
     9601 cttccagttg tgcaaacggg ttgttagggt cttgctgagg atgtaccttt gcagaggtgg
     9661 cgcgctgttc gactgttgag tctgtcaccg ggtcaccagc ccattgagca aagcgatcat
     9721 caacgtaacc gcggccttca ggtcctggcg tatattcacc acgctttgcc ttcataacgt
     9781 tgccgggacc gtcgtgataa gcctgaagag cgtcacgcca gttaccaaac tgctggtata
     9841 tctttgccag atagcgcgcg ccagcgtcag cctgatattc ggggttttgc atttgctcat
     9901 cggtataacc catatcacgc catgtcccag gcatgacctg agtcaatcct acagccccgg
     9961 cggagcttac tgcggcaggg ttgtaagaag actccttggc acccagtgca gtcatcaacc
    10021 cttctggcac accgtaacgt gcgccagcct gctctaacaa atcacggtaa ttagccattt
    10081 actgccccaa agatggaaga tatccgtagc gattaatgaa gtcgattgac agctcggggt
    10141 gctgcttcag gtaatctata gaagcctgag gcgcttccac tcgcttgata ccgttttgct
    10201 gaacgtactt accaaccgcc tcattacgct tctggttgag cgtgttcagg atgacgccag
    10261 cgttgcgacg aaaggactcc tcgctctgcg agttctgcag cgaaccaaca gcctggtcga
    10321 gctttttgcc ctcggcatca gaaagtgcgc ccatgcctcg catggcctga accgctgtca
    10381 ggtatgcttg ggatttaaag gtatcaagtc gtgcctgagt gtctgcagcc tgtgagcctg
    10441 gaacgttggg gattactcca cgtaagcctg taatgctctt aagtgaagga gaactaacga
    10501 tatcgttcag agttaacatg ctggttgtga gggtgttgat gccgtctttg tagccatcat
    10561 ttagcgcttg ctgcttctgc tgcaactgct ggttgttggc tgctatgcgg ctctgtattt
    10621 cctggcgctt caggtcgtta gtttctgctg atagcatccg gtcaaggcgc ttattttcgt
    10681 tgttaatgcg gtttgtttct gcgtccagat taatgcgctg ctgacctaaa ttcgcctgga
    10741 tatcttgccc gcgcattgtg attgcctgat tccgagcggc ggtttgcgaa tccaggtcct
    10801 gaccgcgcat ggtaacctgg cgaccctgca ttttatcctg taggtcaaag tatttttcgg
    10861 gtccgagact gttcatcccc aggtgatcga caaattcgcc gaactgccgc gggttctgtt
    10921 ggtacatctg agcgacgtcc tgaggattaa cgccaacacg agctaactca ccggcgttgt
    10981 tttgcagcca tgattgcatt gcttctggag acgatgacgc aaggcgtgcg ccagccgcta
    11041 aggtgccgat agaattacgc tggtcttcat caatgaatcc catgccttta cgaacggatt
    11101 caatctggtc tggatattga gtagccaact gacgcaaagc accgcgatca ccagacgcat
    11161 aagcattagc gtacgcctgc tgaaattctt tctgccgctg agcctgcttt tcctgctgaa
    11221 acacccccgc aatacctgaa aggccttgca aagcagtcag cccaacattg ttagcgcctg
    11281 aacgctcaat atcattgttc tgcctgataa gctgaagcgt attgccgatg tcatttacgt
    11341 tcggagcgtt tgagttgacg ccgccgatac cagccaataa tccgccattt gatccttgcc
    11401 aagtagccat gattacccct taaaacaacg agccaagcaa tccgatacca gcaccaatgc
    11461 cagcgcccca aggtgttgat gttcccaaaa ggctggcaag acctgcaccg gcaatcgcac
    11521 cagacgttcc gccactaatt gctgtctgaa gacttgatgg tttgttggca ttagcagcgg
    11581 caagagctgc gctttgctgt gcaatgctgc tcatgttgtt ggcgtatgtc tgcccggcgt
    11641 ttgcctgacc ttgcagcgca ccaagcccaa cgtttgccag attgttgtaa ttgctcatct
    11701 ggtttgataa ccaagactga ccgagtgtcg gcgcgatcgt agccagttga ttgcttgtgg
    11761 ctgtcgaacc aagtccaccc gtcgcctccg cagcagcaag actctggtaa cgagcctgac
    11821 ctgcaaggtc tttatactgc tgagagttgt aatactgatt aagtgcctgc ccctgacctt
    11881 ctaaactgga aaggttctga agctggttaa catactgctc cgcaagaggc gtgaacggag
    11941 caaggttttt catgatcgtc tgccactgct gattttgcag gtctgcggca tacttctgag
    12001 cttctgctgc atactttgcg cttttatcag agctgccacc tttcccgcct ttttcagggc
    12061 aataaggttc ctcgccgcgc agttttctgc ccagcttaaa tgcatataac atggctatct
    12121 cccgtgattc aggaagtcga ttagttcttc gcgtgtggcg ctgtaaaacg tcacgtcatc
    12181 cacgcctttg aagtatttct tgatggttcc tacacgctta aggccaatca ttgcgcagta
    12241 catctgaccg tggcggaatt tgcgcgcagc gaacgatgtg acgcactgaa cggtggtgtt
    12301 agtcagaatg tatcgccaga acgccagccc gatttccttg ctgaatccac gaatctctgg
    12361 caggtacatg gcgtggcaat caaaggttag cggctgaatc tcctgatagt aaacaattcc
    12421 gccgaactgc ccgtgcacgt taacctcaaa gtaacggcat tcaggcttgt agtcgtatcc
    12481 atcgccgttg ttgctcccgg cgataatgtc agtgtgattt cctactgctt cgatcaggtc
    12541 gatgtttcgc gttggtttga actgaatcat cactgctccg cgattatctt gatggttgtg
    12601 gcagtaaacg ccgcaccatt tgactgaata gttaacgtac tgccatttga ggcaaga
//
LOCUS       DAJTNM010000112        12471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTNM010000112 DAJTNM010000000
VERSION     DAJTNM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.112"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..1912)
                     /locus_tag="OO172_003225"
     CDS             complement(<1..1912)
                     /locus_tag="OO172_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5513306.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMK"
     gene            complement(1997..2333)
                     /locus_tag="OO172_003226"
                     /pseudo
     CDS             complement(1997..2333)
                     /locus_tag="OO172_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2333..2689)
                     /locus_tag="OO172_003227"
     CDS             complement(2333..2689)
                     /locus_tag="OO172_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5513307.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2689..4185)
                     /locus_tag="OO172_003228"
     CDS             complement(2689..4185)
                     /locus_tag="OO172_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5513308.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAH
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4182..4343)
                     /locus_tag="OO172_003229"
     CDS             complement(4182..4343)
                     /locus_tag="OO172_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5513309.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4353..4916)
                     /locus_tag="OO172_003230"
     CDS             complement(4353..4916)
                     /locus_tag="OO172_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513310.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLSNERDEKGQWAAYDAVHDVRRELWKALLG
                     WMPDPQGGEIVYVGGTLLDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLS
                     IDVDYIDPGTGPDGDIEHHLEMRFPQN"
     gene            complement(4913..5437)
                     /locus_tag="OO172_003231"
     CDS             complement(4913..5437)
                     /locus_tag="OO172_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513311.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5403..5819)
                     /locus_tag="OO172_003232"
     CDS             complement(5403..5819)
                     /locus_tag="OO172_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5513312.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5816..6139)
                     /locus_tag="OO172_003233"
     CDS             complement(5816..6139)
                     /locus_tag="OO172_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5513313.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6184..7287)
                     /locus_tag="OO172_003234"
                     /pseudo
     CDS             complement(6184..7287)
                     /locus_tag="OO172_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(7288..7914)
                     /locus_tag="OO172_003235"
                     /pseudo
     CDS             complement(7288..7914)
                     /locus_tag="OO172_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
     gene            complement(7892..9133)
                     /locus_tag="OO172_003236"
     CDS             complement(7892..9133)
                     /locus_tag="OO172_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5513314.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9133..9315)
                     /locus_tag="OO172_003237"
     CDS             complement(9133..9315)
                     /locus_tag="OO172_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513315.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9327..11084)
                     /locus_tag="OO172_003238"
     CDS             complement(9327..11084)
                     /locus_tag="OO172_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5513316.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSAGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11084..11566)
                     /locus_tag="OO172_003239"
     CDS             complement(11084..11566)
                     /locus_tag="OO172_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5513317.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11714..12064)
                     /locus_tag="OO172_003240"
     CDS             complement(11714..12064)
                     /locus_tag="OO172_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5513318.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12107..>12471)
                     /locus_tag="OO172_003241"
     CDS             complement(12107..>12471)
                     /locus_tag="OO172_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5513319.1"
                     /translation="EQRDKNARELKLANATITDMQQRQRDVNALDAKYTKELADAKAE
                     NETLRADVAAGRRRLHIKAVCQSVREATTASGVDNATSPRLADTAERDYFTLRERLMT
                     MQMQLEGAQEYIRTQCIK"
BASE COUNT         2787 a         3709 c         2902 g         3073 t
ORIGIN      
        1 ttttcataaa gggatcgcct gttttcggca aatcgataac gcgcattcca ggcggcgcgt
       61 tgtcaaacgt caccttaagt tcgctgcggg ttgccggtga agatgcaagg cttaacagct
      121 gattcctttc cagtgataca ttcggggtgt agcgtttatc gggagataaa tacccatcac
      181 cacgtggcat ctgccatccg gtcatgtcat agacaaaatc atggaactgt ttgccccact
      241 cgtcgaactt atcattcaga ccaaatccac tgttcagtgc atcagccaca aaatttttaa
      301 caagccaggg atgttctttt tcaaactcct gcgccatcat gcccagttca agcaatccac
      361 caatcagact gatctttccg aggcccttta atttcagccc tgtttttcct gccgccgttg
      421 tccattgatt ctgggcgact gttgccgcac gaatacttgc caccgccttc acaccaatat
      481 acagggcaga aaccgttgct attgttttta ctgcctgttc ccatcccccc attgctctga
      541 caacctgatc aacctcctgc cagacttttt tcacaaccgg accgaccgtt tcccagttat
      601 caattatgag atatgcacca ccgacgagaa gcgcaattaa tccctttgcc ggcgtcatat
      661 tcatcacgct acccatgatt tttgttatgc gtgtcaatgt gccaatcgcc acgcccatcg
      721 tcagtaatgc cgcaccagtt ttcgcgatgg tttttacgac ctcaggattt tctctgacaa
      781 acgtgcgcac ttcttccaga aaaggcttca tctcttttat gccttcattg agtgaaggca
      841 ggaaggtttc ccccagcgtg gaagaaatcg cattgatctg gttctgcagc agcaacaact
      901 gattttctgt cgtcgcggca cgggcggcat attctttctg cattgagccg ccatactgct
      961 gcgcatccgc cacacgatta aagttggttc gcaacaaatc cagattcgtg agcagcggcg
     1021 caatggcccc cagggattcc ttcccgaaca gcgctttcag cacagcagcc tgtttttctt
     1081 tcggcacttt tgccagagaa tccagtacgt gcagcatggc cccgcgggcg tctttctgca
     1141 tatccgccgc cagttttttc gggctaatgc gcaacgagcg gagcactttt ttctgcgatt
     1201 tggtggcaga atccctcgct gtcagcgaca acataaaatt ctttatcccc gtcgcagcaa
     1261 tttctgactc cacccccatt ccggcaatgg ttgcccccat tgcggcaatc tctccggagg
     1321 ccacacccgc aacactgcct aaagggccaa tacgggtcac aacatcagaa atctttttcg
     1381 cacttgcagg accggtattg ccaagatagt taatcttatc cgcaagccct gccacctctc
     1441 cctgtgtcag tttaaaggca gtgcgccact gcgccatcat ctgaccggat tcttctgccg
     1501 tggcgtcaaa ggccacgccc atcttcacgg catcgtcagt aaactgcatc agctcgtcac
     1561 gggcgatacc agcctgacca cccgccgcca caatttcggc gataccttcc gccgacatgg
     1621 gcagttctgt tgacaggtcg cgcacctgct ccgtcattgc cttaaacgct tccggcgtat
     1681 ccagaccatc caccactttc cggacatccg ccatttttga ttcaagggca atggcagatt
     1741 tgaccgggag cgccagcgcc cccagtactg cggttccggc acccgctgcg cccagtgaca
     1801 ggctggcaaa ctccttttta aaacctttca gctggcgctg cattcccttc agcggtgccg
     1861 ataccttatc cacggcagtg atgatcgcct tcagctgaaa actgtcagcc atgcttcatc
     1921 tcctcgttaa tgcggacggc ctcggcttcc agttctgtaa actgcgaaac agccacccgt
     1981 cgcagctcca gtggattcag tttccagaac caggcaacat tgtagagtcg tttcccggag
     2041 gtgcttcccg tctccgactg ggtaaaaaaa cgcaggatct gcatgctggt cttaaaaata
     2101 tccagttttg ccatctgcgc cgccgatgaa cgcgggatcc ccgccagcaa cgggatatat
     2161 ttcagtgcca cctggctgtc cagtttaata ctgccttcac cggaaataat gaaaggaaaa
     2221 cccagcgcct cgatttcgtc atacgtgggc tcgcgcaact ccagcacatg cagcatttcg
     2281 ttatgcgctg tcaccggttt ttttaaaact atttctgtaa cgttcattgc cattactgat
     2341 accctccctc ttcgccgtgg aattcaagat ctgccgtgcc ttcttcggca ttatgattag
     2401 cctctccgtg cagccatgcc gccgaaagca catacaccat gccgtttgcc agctcagcgg
     2461 taatcgtcat ctggtctgag gtggtaattt tgtcgaccgg aaagtttttg ggcactttaa
     2521 acgtgccctt aacgtaaggt gaccgccagg tctccttgta atccacatcc cctgccatgc
     2581 cgacgatgtc atcccggaca ttggtgttca tcggcacttc aatgccacct gtcagcgata
     2641 actgctgacc atccacttta aaaaaacagg ttccggcgat ctttgccatt atgctgactc
     2701 ctctgaatac tgaagacgga actggttaac caccgcaaag acacgtaact gattaacata
     2761 atccggcggg aacagcgtat tcagtcggtt cgggttatct gcatcacgct caactatcag
     2821 atactgttta aacagatcgt aattttccac aataccggca cgctccatct gacgatatgt
     2881 cgccagaagt tcccctttga taacggcagg agtgacaatc gcctgccccg gaccaaaacg
     2941 ggtaccatca tttgccagct tgtgacgtcc gtacttgctt gtgatgaccg atttcagttt
     3001 gcgaagaaca tgtgcactgg tatgcagagt ttcactgtcc agatagctgt tgtctgccac
     3061 gccatacgca ttctttttgt aggtggttac ggaacgctgg atctgcaacg tgccgccttc
     3121 cacataagcc gtcgccacgc cgtgagataa aagggtctgc tgctctgtca tgatgaatcg
     3181 cttacctttc ggtgccggaa gcatccccac cagctccccc gtctgtgtcg gacgggcagg
     3241 atcattccgg ataaataccg cttcacgggc aaggcgactg gcaaccagtt catcgacagg
     3301 cgactgggtt tctttttcgt aaccggcaag cgtgatatgt tgctgattat gcatatctcc
     3361 ggcaccaacc agctctgaca gcgttcccag ttttgcggta tagacatgcc cgtataactg
     3421 gcgcgcataa ctccagcgac cgctgctgtc attcatttcg gtcatcatca tattgatgga
     3481 ggcggcatcg ttgaacggca gaccgataaa gtcgaatgcc tcatcgccca tagcagcaac
     3541 agcggcggta agatcaggcg ctccgctacc ggcagcaccg acttccgtca ccacccgaag
     3601 ccctgctggc agaatctcac caccaccaga accataataa ttcagacaga ccggtaactc
     3661 attaccatac agccccttat gacgggcagt cagcgtcacc acgcctgcat cagatgacgc
     3721 cgtaaacggc agggtgataa ccccgtttac cgcttcctta atcgcggtgg caaccgcagt
     3781 ggcatcatcg ccattcacca caggcacctg tacacgggag cgcccgacat acagactcag
     3841 ggtgccgctt tcctctgctt ctccggcaac cgtcaccctg accgtcgccg ccgcccctct
     3901 ggcttccggt actgcaataa catacagttc accgaaagga tctgtctgac ggtagacttc
     3961 caccatacgc gccagctggc tccccgcccc acaaatctgg cgggcataat ctgccgatgg
     4021 catcagcacc aggctgttaa cctcaatggc tgcatcgttg ctggcatgcc cgatcagtaa
     4081 tgcaggcgcg ctggtcaccg ctgtatttgc cgcagaattg ttcatctcgg cgtagaacaa
     4141 cggcaccagc gtattcgacg gaacagtatt aaaacttatc gtcatgattt tttagcctta
     4201 tcctgtttaa cacgtaccac atcacccgcc gctatacggc ggaaccagta gctgctttct
     4261 tccacatttc gcccttcgga aggcaaaagg tctccacggg cagggtcagg aaccgaccgc
     4321 cctttcaggg gtttcacaaa catgagggac tcttaattct gagggaaacg catttccaga
     4381 tggtgctcaa tgtcaccatc cgggccagta ccaggatcga tgtaatccac atcaatactc
     4441 agcagggata aatccggcag ggcattcacg tcctctgcct gtcgcgtgtc ttcttccgtg
     4501 atttcatact tcgccgtaaa atcaaactgg taatacagtt cgtaacggtt cagatccagc
     4561 agggtgccac cgacataaac aatctcgccc ccttgcgggt ccggcatcca tccaagcagg
     4621 gctttccaga gttctctccg gacatcatga acggcgtcat aggctgccca ctgccctttt
     4681 tcatcacgct cattgctgag caccacaatc acggagaagc cttcagtcaa atcctgccag
     4741 taatcggtct gtgatttttg ttcccccggc gcatcgtcag agggaaccac ataggcagca
     4801 ggcaggcgaa gttttccggc atcagggatc gccttaaact gcgccgcgcc accaacacgc
     4861 tcctcaaaac gcgggcatcg ctcacgcagc gccgcaatta tcgttgtcag tttcatttat
     4921 gcttcctttt taccggacgt aacgaacgct gcagctcact ggacaacagt tcctgcgtcc
     4981 agtgacgctg acgctcaata acgtcagcca taaagttatt acgcggggcc agccggaaag
     5041 tcgaagaatg gtgcttcttc tgccgcttat cctttttatc cattccatac gctgaatggc
     5101 gaacgccgta atacagaaac gccggataat aaggagcacc ttcaggaaaa cggcgattcc
     5161 cctgcccgtt tttctggtca ggggaaattt tcaccatcag tccggggcga cgcgtcgttt
     5221 ttttgggaac gtaataacca atggagcggg ccagacgccc ggtctgatat cccgggttct
     5281 ctcccggacc ggagcgtccg cgtttaatca ccagacgtct ggcatcacgc atgtaaacgc
     5341 gcccgatttg cacaaacgcc cgacgcagac gggcgcgatt aaactccagc tcctctggtt
     5401 gtttaaagtc gacgtgtaaa aatgctgtct gattcatggc attcaccccg tcgtcgcgct
     5461 gtacgcagtt cttcacattc cagtaataaa aaacgccgct gaccgttcag gtcacgtatt
     5521 cgccggatcc ggtactcttg gccgtaataa accacttcgt gatctgccgt gatgtcgtgc
     5581 cggaaacgga tcgtaaaata atgcgtaacg atattttctg tctgcactga gccctgataa
     5641 gcggcagcgc ctggctgagc cacctttgcc cagacatcaa acgactccgg atacgttggc
     5701 tccgtaccaa aatcagcggt gggttcatcc acacgaaggc ggatctttat ccggcgattc
     5761 agctctccgg gatccggtaa aaggtaagtg gcactggtct gactttgcct gattttcata
     5821 gcgggacaat cctgtaaggg ccagcaagcc atctgaaact cattggcgtt tccagtttct
     5881 ccacatcggt gatcgttgag cgattttcat aaaaatgact gaccagcatc agcattgcca
     5941 gacgaacgtc atcagtcaga tgcatcccgt caggatcatc ttccggaatc gtctcttccg
     6001 gtgcatacaa cttcctgttc aggtatgtct ctgttttttt ctgtaccgcc tgtgccagca
     6061 gtttcagaaa atcgccgtca ctgtacaaac catcatcgag ccggagatga gatttaatct
     6121 cctcttcttt caggagcata ttttcctcct gtgcccgcga ttacgcgggc acaaaaaacc
     6181 gcattacgca gcggctttct ggcgggttgc agccccaatt ttcatcagct taatcgcctg
     6241 agaatccacc agcataccgc cggttcgctt ggtggtataa aaacccacga acggtttgtt
     6301 ggtgtacggg tcacgcagga tacgggtacc gatgcgatca acgatggtat agccgcgttt
     6361 gaagttccca aacgcaatgg cttttgcatc ggcggcaata tccggcatct gctcattctc
     6421 aacgatgcca taccctgcca gagaagaagg ctgacccagc tcaatacccg gacgccacaa
     6481 ataatttccg tcgttatcct tcagcagacg aatggcaaac aggctgctgt tgttcatcat
     6541 gaacttcgcg ccgctgcggt gcgccttacg caaggtgtaa atcagtttaa tgatcgcatc
     6601 agcggtcacg ccggaagccg cgccggaagc aatgtgctga agtttgccaa acgcacgggt
     6661 cttgtcatct tcatcggtgg actcataagc cagaaaacct tttggttttt tgctaccgtc
     6721 gccactggta aaggcaattt cttcctgttc ggcaaattcc agcgccagct cactgttgat
     6781 ccagtcttcc acattaaaga aagcatcatc gagcattttc tgtgtggcct gcgggttgcc
     6841 gtagatttcc cccataaagg gctcaatcag tcccagtttt gaggtggcgg tttccggacg
     6901 cgcttccgtt tcccccaccc atccggacgt cgtgccgccc agattcacca gttttttata
     6961 atccgagcca ctcagagtga ttacggtggc ctcctggcgc atcaccactt catctttcag
     7021 caacgtcaga atggtgcgat ccagttcttc cggaatggca taaccaccat cttcatcatt
     7081 acccacctgc agtgcctttc gctcaagctc acgcaggcca tcttcacgcc ccttgcgcat
     7141 aaaaccgata aacgcttctt tatgttcccc ggcaacttta ttttgcgtac cacctgccgg
     7201 acgcttaact tcagccagct cagcctcaag atcgcttttg agattttcca gttcagtcag
     7261 ttttccgttg agggtttcca cttctccgtt cagtgcagag cccacatcct cagcgtcgcg
     7321 cagggacagt gcgccatagc ccccggccat gaatgctttg gcctgggtac gggagagtcc
     7381 gacatcacgc aggactcttt cgattttttt ctgttcgggg atttccccgc gggccagtgc
     7441 gttcttgacg tcgctgatcc gcgcctcgtc gttagacggg aacgtcacca gactgacttc
     7501 ccagaggtcg atttctttca gcagaaaggc ttctttgctc cggtcgtatt cccagtcttt
     7561 caggacgtac ccaatagaaa ggccggttaa cgaaccggcc ttcatgtgtg catgtgcacg
     7621 ttttgcgagg ggatcatcat caataagcaa ccgtcccctg acgtaaagcc cgacatcgtc
     7681 ttccttcatt tcggtgtaaa caccgatggg ttcatccatg cggtgctgcc agagcagcgc
     7741 aggtaacgct tttctgtcac tccacgcccg cagggaagca gcaaatgccc cggacatcac
     7801 cacatcatcg tggctgtcct ttacaccaaa gacggagcca tgcccttcaa actcaccgga
     7861 gtcactgaca gatttcagac tcagcggtac atcaagacgt tgtttcgtct gcattggcgt
     7921 tatccttctg cttaccggct ttactgccat cggagggttt cgtggtcatg ttcatcggtg
     7981 tgagatagac atccccaccg ggacgcggat tcatatcttc caggtcgcgg cagtcattgg
     8041 gagagtaaat cccccagtta atcccggtgg cgtaggcttc aaaacgggat ttcatatccc
     8101 cgcgcagtaa cgccccggcg ttaaatttgg cgtaaaactt cccctgtttg ctctccctga
     8161 ccagccctgt attgatccgt tgttcaatac gggtcagata cggcacaagg gaatagttga
     8221 taaatccgag ccccagctct tcaatattgt tgaaagtggc gcgatcggtg ttctgcacca
     8281 tgtgcagcgg cacgcggaaa agacgacaga tttcttccaa ctgaaacttg cgggtttcca
     8341 ggaactggct gtcctcggca ttcagtgcca ccggcttcca gtccagcccc atttccagaa
     8401 tcatcggacg gtgcgcatta cccagcccaa gatgacgctc ctcaaaatcc ctcttcatgc
     8461 gctcataagc atccggcgtg agcttttgtt ccgtgcgcaa cacaccggat gtcaccgcac
     8521 cattaccaaa caacctggca ccgtgctcct cggttgccgc tgccagtgaa atggcctcgc
     8581 gcgcatatgc aatgggattc agcccgacca gtccgtccag cgtcagggtg cgcacatgcc
     8641 agatttcatc ctgggtcaac acatccacgg aaccatccgg aaacgtcacc tgataaaccg
     8701 gctgccactg gctgttcagc ttcggttcca cacagcccgg atctatcgga agaagctcca
     8761 caacttcccc cagcgccttc accttgtaag cgtaaaaatt cccccgcaga cacaggcaga
     8821 cgataaccag ctcccagaat tcctgcggcg tcatgtagcc attgggtttt gctgaaatca
     8881 gcttatgcag ccgttcatcc accgcccgtg ttttaagggt gccgctgatt ttgtagagac
     8941 tgcagggcag cataccaaca gactcagcca gcaccctgac gcaggaatag accgccgtca
     9001 gccgcatggc ccgctggctg ctgatccgct ttccggtata ggtgtcgtat gacaacccca
     9061 gctcttccgc aagcatcccg ggcgttgtga cgggggtgtt atttttgcgt tgaaaaagcc
     9121 cctggaaaaa cattactcac ctccggaggc gacccggtga ccgcgatcga gataacgcgc
     9181 aaccagccac gaccagcaca gacacagcgc cccggcaaca acaaaacctg ccgggggata
     9241 aatcagccat gtgccatacg acagcaaaag cgcccccagc acgcccacca gtggcgtgag
     9301 aattatcaga aacataatga cctcggttaa agcgagcgaa taccaacgct gaccagatgc
     9361 tcagacagat ccggctccgg ttcaccgcca ttgaccagca tccggctcat tgctgtaaac
     9421 atcgcaacag ggccgtcgat tttggcttcc ggcgtggatt tgttcgggaa gatattgtcg
     9481 tttttgtccg gtttcaccgt aacgttagac atcatccagt tcatgaccgg gtgattactg
     9541 tgatggaaac gcccggcata gaccagtgat tccgtttcct tcatggcctc tgacagattg
     9601 cggaccgtct gcggaacttc caccagcggt ataccttctt cagccagcgc cagactgaac
     9661 tgcattgcgc tccacgggtc aaatcccagt tccctcaggt tttcaccgcc aatccattcc
     9721 agtaagtcac tttttatctg agcatgatcg ataacatcac catccgtcag aatcagctta
     9781 tccatctccg cccacttccg gtaaagttct gcctgctgcc gcgagcatcg ttccagccgt
     9841 ccttccggaa gccagaattt aaaatcggca tgaacatgcc cgttatccgt tcgccagagt
     9901 tttgccgccg cacagatatc aatcttatga gcaaggtcaa cgccgaccca catgggatac
     9961 gttttcagct catgtcgtgg ggcaatgtat tcgcacttct cccacttaat catgtccatc
    10021 caggcagact ctgctgttac ccacacattc atgtgtttgg taaaaaaatt cacccgcgca
    10081 gagacctgtt ctttcgcttt tttcgccagg cgacgcagat catcccagcg tttacagatg
    10141 cccaggccgg gattcgcttt ctgccagacc gtttcatcaa acggatcatc tccctcatcg
    10201 agggtgtaaa taatcgcaaa gtaggagtcg tcttttaccg cgccctccac gtcgctgtta
    10261 tagccacgca ataccttgat ggcgtaatca cgctgctcgt aacaaattcc ttccttgtta
    10321 aaccctgccg tggtgatacc aaataaaagg gactgcagac gggcaccggt tgccgtttcc
    10381 agaacgtccc acacgtcacg ggttttatgt gcatgcagct catcaataat ggcgcagtgg
    10441 atgttcagac catccaggtt gtttgcatct gaagaaagcg gttcaaattt tgatgcgctc
    10501 tgctcctgat aaatcgccag cttgttgaaa tcaaacaacc gcccgagtgt cgaccgggct
    10561 tttctgacca tatttttggc gtcttcaaac acgattctgg cctggtcacg cgtggttgcg
    10621 gctgaataca cctcagcccc gccttcacca tctgcccccg tcatatacag accgatacct
    10681 gctgacaggg ttgattttgc gtttttacgg gcgacttcgt tgtacgctgt ccggaaacgg
    10741 cgcaccatca ccgggcgtcc gctgccatcg ctgcgcatga caacttcccc ggtctcttca
    10801 ttcaccagcg gaatgacaaa accaaaaata ttaatgagga taaacacatg ccagtccatc
    10861 aactcaatgg gctgacctgc cagcgcccct tttacatggg gcacaaattt gtagaaattc
    10921 aggatgtgct gcgcacgggg ttcactgaaa taaatccccc gctcttcgcc gtacttcaga
    10981 tcatcaagaa aacgctggca ggccagacgg acaaattcac cagcgacaat ttctcctgca
    11041 acaacacgtt cggcgtagcg gatcccgtca gccacttttg ccatcagtct ctcgctttta
    11101 aaagttctgc cagcggatca acatcatccg gtccggcgat atttactttc gcccggcttg
    11161 ccggtgacat accaaattct gcaagcatcg cccggatccg cttccaggca tccgctttca
    11221 tcgcagcagc cgggtgtgcc ttaatcagca catcaccgtt ctgcgtttcc gtgcggtagg
    11281 tataaccctc aacatcgagg gtttcgcagt gatgccggta ttcggtgtag gcttccacca
    11341 gcaactcgag tgcacgcgca tcaagctgag aaatgatccc ttccgcattc agctcttccg
    11401 ccattcgcct gaaccagtac tttccctgcg cccctaaatg ctgcggaatt ttagggagac
    11461 ctttttcgtc ctttttagcg gtttttttgg cgtctttaac gggacgcttt gagggattgc
    11521 ctcgtatcaa atgcaggcgt ggcggggttt tcgggggtcc tgacataatc gattttacct
    11581 atcaatcgct tgatcgcatt cccaaaaaaa agttttcgaa cctgcggcga tgcgaggaag
    11641 ggttggcggg cggtcccgga cagccagggc tgcagggatt tgacccgccc ctccctacaa
    11701 gtgagaataa ttatcacctg attcgttcgc gcgctgtttt cgctttatgg cagggccagc
    11761 acagactctg caggttgctg tctgcgtctg ttccgccatg cgctttcggg atgatgtggt
    11821 cgacggtttt cgcctcgctc accacaccga cacgcagaca caactgacac agacctttat
    11881 cgcgcttcag aatacgggca cgaatcaccg tccattttga gccatagcca cgctggtggc
    11941 ggctcagtcc gcgctggtgc tgcacccagc cttcaccgcg atgcttatcg cagtaacccg
    12001 aactgtctgt ggttgtacct gcacatccac gcttacggca ggcacgtggg attagtgatg
    12061 gcataaatac ctcataccct gcgaaatgtt taccacgata aaaaggctac ttaatgcact
    12121 gagtgcggat atactcctgt gccccttcca gttgcatctg catcgtcatc agccgctctc
    12181 tgagggtgaa ataatcccgt tcagcggtgt ctgccagtcg ggggctggtt gcattatcca
    12241 cgccggaggc ggtggtggct tcacgcactg actgacagac tgctttgatg tgcaaccgac
    12301 gacgaccagc ggcaacatca gcgcgcagag tttcattttc agctttcgca tcagctaact
    12361 ccttcgtgta tttagcatcg agtgcattaa catcacgctg tctctgctgc atgtcagtaa
    12421 tggttgcgtt cgccagcttc agttctcttg cgtttttgtc gcgctgctct t
//
LOCUS       DAJTNM010000113        12256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTNM010000113 DAJTNM010000000
VERSION     DAJTNM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.113"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..221
                     /locus_tag="OO172_003242"
     CDS             <1..221
                     /locus_tag="OO172_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5513320.1"
                     /translation="VLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPMSGPLTQAFED
                     ARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            218..617
                     /locus_tag="OO172_003243"
                     /pseudo
     CDS             218..617
                     /locus_tag="OO172_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            628..1368
                     /locus_tag="OO172_003244"
     CDS             628..1368
                     /locus_tag="OO172_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5513321.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1427..1813
                     /locus_tag="OO172_003245"
     CDS             1427..1813
                     /locus_tag="OO172_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5513322.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1822..2151
                     /locus_tag="OO172_003246"
     CDS             1822..2151
                     /locus_tag="OO172_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5513323.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2123..5062
                     /locus_tag="OO172_003247"
                     /pseudo
     CDS             2123..5062
                     /locus_tag="OO172_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5062..5391
                     /locus_tag="OO172_003248"
     CDS             5062..5391
                     /locus_tag="OO172_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5513324.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5391..6089
                     /locus_tag="OO172_003249"
     CDS             5391..6089
                     /locus_tag="OO172_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5513325.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYDRFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6094..6837
                     /locus_tag="OO172_003250"
     CDS             6094..6837
                     /locus_tag="OO172_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5513326.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6774..7375
                     /locus_tag="OO172_003251"
                     /pseudo
     CDS             6774..7375
                     /locus_tag="OO172_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            7436..10915
                     /locus_tag="OO172_003252"
     CDS             7436..10915
                     /locus_tag="OO172_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5513327.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLERWDTPKVVKGVSFLLRLTVAADDGSERLVSTGRTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDYAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKTLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTTNKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            10983..11582
                     /locus_tag="OO172_003253"
     CDS             10983..11582
                     /locus_tag="OO172_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5513328.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11646..>12256
                     /locus_tag="OO172_003254"
     CDS             11646..>12256
                     /locus_tag="OO172_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5513329.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPWYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGT"
BASE COUNT         2886 a         2912 c         3917 g         2541 t
ORIGIN      
        1 atgttctgag gtgtcttcct gaagcgcgtt ttgcaacagg gcatgaccat cagggcaggc
       61 taagaaaaaa tcgtcttcct gtggaggtag tgaaaatccc gatgtccgga ccgctgacac
      121 aggcatttga agatgcccgc gatcgcatca ttgctgcgga aatgccgaaa cagctggggt
      181 atgccctgaa acaacaactg aggttacatc tgagtaaatg aaccggcaca cacaaatccg
      241 tcagagcgtg ctggcacgcc ttcgggaaca gtgtggagac agcgccacgt tttttgacgg
      301 gcttccggca tttattgatg cgcaaactgc ctgccgtggc ggtgtggctg agtgatgctc
      361 agtacaccgg aaaaatgacg gatggagatg actggcaggc tgttctgtat attgccgtct
      421 tcatccgggc acaggcaccg gattcagagc tggatatgtg gatggagagc accattttcc
      481 cggccctgaa tgatgtaccg gcactttccg gactcatcga caccctgaac ccactcggtt
      541 ttaactatca acgtgataat gagatggcta cctgggcgat ggcggaaatc acgtaccaga
      601 tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc aaaaacgaaa
      661 ggtgcgggaa cgacgttatg gatgtatacc ggcaacggcg acgcatttgc gaaccctttg
      721 tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg cgagatgacc
      781 gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa aacgacaacc
      841 caggggcaga aatccgtcgg tgatacttcg gcgacgctgg cctggcgtcc gggtgacagc
      901 gggcagaaaa aactggttca gttgttcgat tccggtgaag tctgcgcgtt tcgtatcaaa
      961 taccccaacg gtacggttga tgttttccgt ggctggctga gttcactggg taaaaccatt
     1021 acctcaaaag aggtgatgac ccgtactgta aaaatcagcg gtgtggggcg tccgtatctg
     1081 gcagaggagg gcgctgaaac cgtgggtgtt accggactga cggtggcacc ggcatctgtc
     1141 agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga cggggccagt
     1201 gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgcc cctgagcggg
     1261 cttgtggcca cggtgaaagg cgtgcagcag ggcagtgtca gcatcgtggg catgacctct
     1321 gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca ggacgatact
     1381 catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt ttctgaaaca
     1441 ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg ggctgcagag
     1501 aattgaatat ctgacgtttg ttcagcagcg aaaggcaaag tttgatgcac aggagggaga
     1561 actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata tcaatgcctg
     1621 gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga cgctttgcgc
     1681 atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga gggttctgtc
     1741 gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg cgctgacgcc
     1801 ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga gttccggcgg
     1861 gcagactggc ggcagatgct gtcggatatg tcggccactg agcttggtga gtggggcgat
     1921 tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc ctcgctgaag
     1981 gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga tttcagcctt
     2041 ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca tcttggggaa
     2101 ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg atttggggat
     2161 tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga atggtgcagc
     2221 cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc agactcaggc
     2281 ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag cccatgcgca
     2341 gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg aacaaacccg
     2401 tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg ccagggcact
     2461 ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca gtgtgaaaca
     2521 ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac aggccagaaa
     2581 cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca cggcgaaaac
     2641 ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt ttatccgcca
     2701 gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc gcgccagggc
     2761 ggctcagctg ggggtgttaa tcagtcagat ggcgcgcggc aattttggtg cgctgagggg
     2821 atccgggata acgctggcta accgtgccgg atggacaggc gcactgatgt cgccgaaagg
     2881 catgatgact ggcggcgtta ttggcggact tgtcgcggcg gtcctgggtc tgggtaaagc
     2941 ctggcatgac ggcggaagga gggcgaggaa tttaaccgtc agctggcgct gacgggacat
     3001 tatgccggtg tcactgtcgg gcagttgtgg aaactcagcc gggccatatc cgggaatggt
     3061 atcacgcaac atgcggcagc cggtgcgctg gcacaggtgg tgggcagtgg ggcatttcat
     3121 ggaaacgata ttggtatggt ggcgaaagct gccgcacaga tggagcgatc ggttggccag
     3181 tcggtcagcg ataccataaa tcagtttaag cggctgaagg atgatcctgt aaatgccgcg
     3241 aaggcgctgg acaatgcgct gcattttttg actgccactc agcttgagca gatacgcgtc
     3301 cttggggagc aggggcggtc cagtgatgcg gcccggatcg ccatgtctgc gctggcagag
     3361 gaaaccggta aacgcacatc tgatattgat aataatctca atgcgctggg tagtacgctg
     3421 caaaccttgt ctgactggtg gaagcagttc tgggatgcgg ccatgaatat tggtcgtgaa
     3481 gactcgctgg atgcgcagat tgatgcgtta caggaaaaaa ttcagcgcgc gaaaaaatat
     3541 ccgtggacaa acgcctccac acaggtggag tacgatcagc agcgtcttaa cgatcttcag
     3601 gagaaaaaac gccggaagga tttgcaggat gcaaaagcgc aggcagaacg gaattaccag
     3661 gagcaacaga aacgccggaa tgctgaaaat gccgcgctga accggatgaa tgaaacggaa
     3721 gctgcacgac atcagcggga aattgcgcgt attaatgcca tgcagtacgc cgaccaggct
     3781 gtcagggatg cggcgataca gcgtgaaaat gaacgttacg aaaaagccat taagaaaaat
     3841 acacgggcaa cccgtaatga tgaggccacc cggttattgc tgcagtacag tcagcagcag
     3901 gcacaggtgg aagggcagat tgctgctgcc agacagtcag caggcattgc cactgaaagg
     3961 atgactgaag cgcataaaca gcttctggcc ctgcagcagc gcatcagcga cctggacggg
     4021 aaaaagctga cggcagatga aaagagtgtg ctggcccgta agaatgagct gattcaggcg
     4081 ctgacgctgc tggatgtgaa acagcaggag ctgcagaaac agacagcgct taacgacctg
     4141 agaaaaaaaa cggttcagct gaccagccag ctggcagaca aagaacgtgc actgcgtgag
     4201 cagcacaatc tggatattgc cactgcaggt atgggggata agcagcggca gcgctaccag
     4261 gcacagttgc gcatccggca ggaataccgg caacagttgc aacagcttga gaatgacagt
     4321 cgccagaaag gcacttacgg gacggaggac taccggaggg ctgaggaggt gctgaagggg
     4381 agcctgaagc gacaactgaa tgaaaacaaa cgctactggc aggaactgga agtggcgcag
     4441 ggcgactgga aaaacggtgc catgcgggcg tttcagaatt ttacggcgga tgcggataat
     4501 gcggcgggaa cggcagaaca gatgtttaca gtggcattca gcagtgccgg taatgcactg
     4561 gcgacattct gtaccaccgg taagctgaat tttaaatcct tcacctcttc cctgttgtca
     4621 gatatggcca gaattatggc acagatggcc atgatgcagg cggtaaaggg cgtcggttct
     4681 ttattcggct tcacgactaa tgctgatggc ggtgtttacc agtctgctga tttgagtcgc
     4741 tacagtggca cggtggttaa ccgtccgacg ttttttgctt ttgcaaaagg cgcgggtgtg
     4801 atgggggaag cgggacctga agccattctg ccactgcgtc gcggtgctga tggtaagctg
     4861 gggggttgtg gcggatactg gtggttcagg catggtgatg tttgccccgc agtacaacat
     4921 tgagatcaat aacgacggca cgaacgggca gataggtccg gctgccctga aggtggttta
     4981 tgacctcggg aaaaaagcgg ccgcggactt tatgcaacag caggcccgtg atggtggccg
     5041 gttaagtgga gcataccggt aatggagacg tttcactgga aagtgcgccc ggatatgaat
     5101 gtggtatcag agccgaaagt ggtgacagtg aagctgggcg atggttatga acagcgtcgt
     5161 gcggcgggac tgaataacca gttgtcgact tacagcgtga cgatacgtgt tcgtaaaggt
     5221 gaacacccat ctttaaaagc ctttctggaa cggcacggtg gcgtccgcgc atttcagtgg
     5281 acgccacctt atgactggaa actgatcagg gaggtttgtc gtaaatggtc ggcaagcgtg
     5341 ggggcgttat gggtgactgt aacggccgat tttgaacagg tggtgaactg atgcaggata
     5401 tccggcagga aacactgaat gaatgcaccc gtgcggagca gtcagcccgg gtggagctct
     5461 gggaaatcga tctgacagag gtcggtggtg agcgttattt tttctgtaat gagcagaacg
     5521 aaaaaggtga gccggtcacc tggcaggggc ggcagtatca ggcatacccc attcaggggg
     5581 cggggtttga actgaacggc aggggctgtg ctgcccgtcc gacactgacg gtctctaacc
     5641 tgcacggcat ggtcaccggg atggcggaag atctgcagag tctggtcggc ggaacggtgg
     5701 tccgccgtaa ggtttacgac cgttttctgg atgcggtgaa cttcgtcaac ggaaacagcg
     5761 acgccgatcc ggaacaggag gtgatcagcc gctggcgcat cgagcagtgc agcgaactga
     5821 gcgcggtcag tgcctctttt gtgctggcca caccaacgga gacggatggc gcggttttcc
     5881 cggggcgtat catgctggcg aatacctgta tgtggactta ccgttctgat gagtgtggtt
     5941 acacgggcag ggcagtggct gacgagttcg acaaaccaac gacggatatc cggaaggaca
     6001 aatgcagcaa gtgtatgcgc gggtgtgagt tgcgcaacaa taccggtaat ttcggcggtt
     6061 tcctttccat caataaactt tctcagtaaa tccatgacac agacagaatc agcgattctg
     6121 gcgcacgccc ggcgatgtgc gccagcggag tcgtgtggat tcgtggtgag aacgccggag
     6181 ggagacaggt atcttcccag cgagaatatc tccggtgagc cggaggaacg gttccggatg
     6241 gctccggagg actggctgcg ggcacaaatg cagggtgaga ttgtggcact ggtccacagt
     6301 catcccggtg gtctgccctg gctgagtgag gctgaccggc ggctgcaggt gcagagtgat
     6361 ttgccgtggt ggctggtctg ccggggggcg attcacaagt tccgctgtgt gccgcatctc
     6421 accgggcggc gctttgagca cggggtgacg gactgttaca cgctgttccg ggatgcttat
     6481 catctggcgg ggattgagat gccggatttt caccgcgggg atgactggtg gcgtcacggt
     6541 cagaatctct atctggataa tctggaggcc acagggctgt atcaggtgcc gttgtcatca
     6601 gcacaaccgg gcgatgtgct gctgtgctgt tttggttcat cggtgccgaa tcatgccgcc
     6661 atttactgcg gcgatggtga gctgctgcac catattcctg aacaactgag taaacgagag
     6721 aggtataccg acaaatggca gcgacgcaca cactccctct ggcgtcaccg ggaatggcac
     6781 gcatctgcct ttacggggat ttgcaacgat ttggccgccg catcgacctt cgtgtgaaaa
     6841 cgggggctga agccatccgc gcactggcca cacagctccc ggcgtttcgt cagaaactga
     6901 gtgacggctg gtatcaggtg cgcattgccg ggcgtgatgc aggtgaaacc gaattgtctg
     6961 cccgtcttaa tgagccgctg gcaaatggtg cagtgatcca catagtaccg cgtctggtgg
     7021 gagctaaaag tggcggtgtg tttcaggcgg tgctgggggc agctgttatg gcggttgcta
     7081 tatggatgcc ggggtaggaa ttatggcgag taatctgctg ttttctctcg gtgccagtat
     7141 gacgcttggc ggtgttgcac agatgctggc accgaaagcc agaactcccc gtacacagac
     7201 aacggataac ggcaaacaga acacctgttt ctcctcactg gataacatgg ttgcccaggg
     7261 caatgttctg cctgttctgt acggtgaaat gcgcgtgggg tcgcgggtgg tatctcagga
     7321 gatcagcacg gcagacgaag gggatggtgg tcaggttgtg gtgattggtc gctgatgcaa
     7381 aatgtttcat gtgaaaccgc ctgcgggcgg ttttgtcgtt tatggagcgt gaggaatggg
     7441 taaaggcagc agtaaggggc ataccccgcg cgaagcgaag gacaacctga aatccacgca
     7501 gttgctgagt gtgattgatg ccatcagcga agggccggtt gacggtccgg tggatggatt
     7561 aaaaagcgtg ctgctgaacg gtacgccggt cctggacagc gaggggaaga caaacttttc
     7621 cggtgttacg gtggtgttcc gcgccggcga gcaggagcag acaccgccgg aggggtttga
     7681 atcttccggc tcagagactg tgctgggtac ggaagtgaaa tacgacacgc cgatcacccg
     7741 gaccatcacg tcggcaaaca ttgatcgtct gcgcctgacc ttcggtgtgc aggcactggt
     7801 ggaaaccacc tcaaaggggg accggaatcc gtcggaagtc cgcctgctgg ttcagataca
     7861 gcgtaacggt ggctgggtga cggaaaaaga catcaccatt aagggcaaaa ccacttcgca
     7921 gtatctggcc tcggtggtgg tgggtaacct gccgccgcgc ccgtttaata tccggatgcg
     7981 caggatgaca ccggacagca ccacagacca gctgcagaac aaaacgctct ggtcgtcgta
     8041 caccgaaatc atcgatgtga aacagtgcta cccgaacacg gcgctggtcg gcgtgcaggt
     8101 ggattcagag cagttcggta accagcaggt gagtcgcaat tatcatcttc gcgggcgcat
     8161 tctgcaggtg ccgtcgaact ataacccgca gacgcggcaa tacagcggta tctgggacgg
     8221 aacgcttaag ccagcataca gcaacaacat ggcctggtgt ctgtgggaca tgctcactca
     8281 tccgcgctac ggcatgggga aacgtcttgg tgcggcagat gtggacaaat gggcgctgta
     8341 tgtcatcggc cagtactgcg accagtcagt gccggacgga tttggcggca cggagccgcg
     8401 catcacctgt aacgcttacc tgaccacaca gcgtaaggcg tgggatgttc tcagtgattt
     8461 ctgctcggcg atgcgctgta tgccggtatg gaacgggcag acgctgacgt tcgtgcagga
     8521 ccgaccgtcg gataaagtgt ggacctataa ccgcagtaat gtggtgatgc cggatgatgg
     8581 cgcgccgttc cgctacagct tcagcgccct gaaggaccgc cataatgtcg ttgaggtgaa
     8641 ctggattgat ccggacaacg gtcatgagac ggcgacagag cttgtggagg acacgcaggc
     8701 cattgtccgt tacggtcgta acgtcacgaa gatggatgcc tttggctgta ccagccgggg
     8761 acaggcgcac cgcgccgggc tgtggctgat taaaacggaa ctgctggaaa cgcagaccgt
     8821 ggacttcagc gtcggcgcag aagggcttcg ccatgtaccg ggtgatgtca ttgaaatctg
     8881 cgatgatgat tatgccggta tcagcaccgg tggtcgcgta ctggcggtga acagccagac
     8941 ccggacgctg acgctcgacc gtgaaatcac gctgccgtcc tccggtacca cgctgataag
     9001 cctggttgac ggaaatggca atccggtcag cgtggaggtt cagtccgtca ccgacggcgt
     9061 gaaggtgaaa gtgagccggg ttcctgacgg cgttgcagaa tacagcgtgt gggggctgaa
     9121 gctgccgacg ctgcgccagc gcctgttccg ctgcgtgagt atccgtgaga acgacgacgg
     9181 cacgtatgcc atcaccgccg tgcagcatgt gccggaaaaa gaggccatcg tggataacgg
     9241 ggcacacttt gacggtgacc ggcgcggcac ggtgaatggt gtcacgccgc cagcggtgca
     9301 gcatctgacc gccgaagtca ccgcagacag cggggaatat caggtgctgg agcgatggga
     9361 cacgccgaag gtggtgaagg gtgtgagctt cctgcttcgc ctgaccgtgg cagcggacga
     9421 cggcagtgag cggctggtca gcacaggcag gacgacggaa accacatacc gcttcaggca
     9481 gctggcgctg gggaattaca gtctgacagt ccgggcggta aatgcccggg ggcagcaggg
     9541 cgatccggcg tcggtatcgt tccggattgc ggcaccggca gcgcctgtca ctattgaact
     9601 gataccgggg tattttcaga taacggtggt cccgaaactg gctgtatatg acccgacggt
     9661 gcagtttgag ttctggtttt cggaaaagcg gattgcggat atcaggcagg ttgaaaccag
     9721 cgcccgctat cttggcacgg cgctgtactg gatagccgcc agtatcaata tcaggccggg
     9781 ccatgattat tatttttacg ttcgcagtgt gaacaccgtt ggcaaatcgg catttgtgga
     9841 ggccgtcggt cgggcgagcg attatgcgga aggttatctg agtttttata aagggttgat
     9901 caataaaacg catctcggca aggagttgtg gacgcagatt gataacggtc agcttgcgcc
     9961 ggacctgact gaaatcagga cgtccattac gaatgtcagc aatgaaatca cgcaaaccgt
    10021 caataaaaca ctggaaaatc agagtgctgc aatccagcag atacagaaag ttcaggttga
    10081 tacaaataat aacctgaaca gcatgtgggc tgtgaagctg caacagatga aggacggacg
    10141 cctttatatt gcgggtatcg gtgccggtat tgagaatacg ccagcaggaa tgcagagtca
    10201 ggtgctgctg gcggcagaca ggattgcgat gattaatcct gcgaatggca acacaaagcc
    10261 gatgtttgtt ggtcagggcg atcagatatt catgaacgac gtgttcctga aacgcctgac
    10321 ggctccgacc attaccagcg gcggtaatcc tccggcattt tccctgacac ctggcggacg
    10381 gctgacggcg aaaaatgccg atatcagcgg taacgtgaac gcgaactccg ggacgctcaa
    10441 caacgtcacg actaataaga actgtcgggt tctgggaaaa ttgtccgcga accagattga
    10501 aggcgatctc gttaaaacag tgggcaaacc tttcccacgg gactcccggg caccggagag
    10561 gtggccatca gggaccatta ccgtcagggt ttatgacgat cagccgtttg atcggcaaat
    10621 tgttattccc gcggtggcgt ttcgcggtgc taaacatgag cggaagaata acaatattta
    10681 ttcgtcatgc cgcctgatag tgaagaaaaa cggtgctgaa atttataacc gaacgaccct
    10741 ggataatacg ctgatatata cgggtgttat tgatatgcct gccggtcacg gtcacatgac
    10801 gctggagttt tctgtatcgg catggctggt aaatggctgg tatcccacag caagtatcag
    10861 cgatttgctg gttgttgtga tgaagaaagc cactgcaggc atcacgatta gctgaatttt
    10921 ataacccaga tacgggcacc agaaatggtg ccttttttat tgcagaaaag cgagaggtaa
    10981 ttatgcgtaa agtttgtgca gccattttgt ccgcagccat ctgtctggct gtatccggtg
    11041 cgcctgcatg ggcgtctgaa catcagtcca cgctgagcgc cgggtatctt cagccccata
    11101 ctgatatgcc aggcagcgat gacctgaagg gcattaacgt gaaataccgt tatgagttta
    11161 cggacacgct ggggctggtg acgtcattca gctatgccaa cgctgaagat gagcaaaaaa
    11221 cgcattacag cgatacccgc tggcatgagg attccgtgcg taaccgctgg ttcagcgtga
    11281 tggcggggcc gtctgtgcgc gtgaatgaat ggttcagcgc gtatgcgatg gcgggcgtgg
    11341 cttacagccg tgtgtcgacc ttctccgggg attatctccg cgtaactgac aacaagggga
    11401 aaacgcacga tgtgctgacc gggagtgatg acaaccgcca cagcaacacg tctctggcgt
    11461 ggggggctgg tgtgcagttt aacccgaccg aatccgtggc cattgacctt gcttatgaag
    11521 gttccggcag tggcgactgg cgcactgacg gtttcattgt gggtgtcggt tataagttct
    11581 gattagccag gtaacacagt gttatgacag cccgctggtt caggcgggct ttttgtgggg
    11641 tgaatatggc agtaaagatt tcaggtgtac tgaaagatgg ggcgggtaaa cctgtcgtaa
    11701 attgtgcgat tgaactgcgg gccagaagaa ccagtccgac cgttgtggca cacgttgttg
    11761 ccacttgcgt gacggacaat aacggtgctt atgtgattga ggctgagccg tggtattacg
    11821 aggttgcgct tcactgtaac ggctggcagc caacccgtgt cggggatatt gatgtggcac
    11881 cgactgatgc accggggaca ctgaacgcgt ttctgaatgc accaaaggat ggtgatttac
    11941 gtccggaggt gatgaagcgc tttgaggaaa tggtggcgca ggcgcagcag agtgccgggg
    12001 ctgcagccgg aaacgcacag cagacggcgc aggatgtggc ggcagccgca acggcccgtg
    12061 atgatgcaca acgttttgcg gagaaagccc gacaggatgc aaccgtcaca gctgaggaca
    12121 gaaaggccac tgcggaagat gtgacaagca caggagcaaa tgcagccgca gccggacaga
    12181 gcgcacagga tgccgcaggt tatgcccgcg cagcagaaca ggccaaaaat gacattgatg
    12241 ctgcgctgac cggcac
//
LOCUS       DAJTNM010000114        12152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTNM010000114 DAJTNM010000000
VERSION     DAJTNM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.114"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(99..1190)
                     /gene="ychF"
                     /locus_tag="OO172_003255"
     CDS             complement(99..1190)
                     /gene="ychF"
                     /locus_tag="OO172_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5513330.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1307..1891)
                     /gene="pth"
                     /locus_tag="OO172_003256"
     CDS             complement(1307..1891)
                     /gene="pth"
                     /locus_tag="OO172_003256"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5513331.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2169..2447
                     /gene="ychH"
                     /locus_tag="OO172_003257"
     CDS             2169..2447
                     /gene="ychH"
                     /locus_tag="OO172_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5513332.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2502..4181)
                     /gene="dauA"
                     /locus_tag="OO172_003258"
     CDS             complement(2502..4181)
                     /gene="dauA"
                     /locus_tag="OO172_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5513333.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4306..5253)
                     /gene="prs"
                     /locus_tag="OO172_003259"
     CDS             complement(4306..5253)
                     /gene="prs"
                     /locus_tag="OO172_003259"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5513334.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5404..6255)
                     /gene="ispE"
                     /locus_tag="OO172_003260"
     CDS             complement(5404..6255)
                     /gene="ispE"
                     /locus_tag="OO172_003260"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5513335.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6255..6878)
                     /gene="lolB"
                     /locus_tag="OO172_003261"
     CDS             complement(6255..6878)
                     /gene="lolB"
                     /locus_tag="OO172_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5513336.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPTNMELTDGGQRIKLKMDNWIVK"
     gene            7092..8348
                     /gene="hemA"
                     /locus_tag="OO172_003262"
     CDS             7092..8348
                     /gene="hemA"
                     /locus_tag="OO172_003262"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5513337.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8390..9472
                     /gene="prfA"
                     /locus_tag="OO172_003263"
     CDS             8390..9472
                     /gene="prfA"
                     /locus_tag="OO172_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5513338.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9472..10305
                     /gene="prmC"
                     /locus_tag="OO172_003264"
     CDS             9472..10305
                     /gene="prmC"
                     /locus_tag="OO172_003264"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5513339.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10302..10694
                     /locus_tag="OO172_003265"
     CDS             10302..10694
                     /locus_tag="OO172_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5513340.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10698..11507
                     /locus_tag="OO172_003266"
     CDS             10698..11507
                     /locus_tag="OO172_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5513341.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11543..>12152
                     /gene="kdsA"
                     /locus_tag="OO172_003267"
     CDS             11543..>12152
                     /gene="kdsA"
                     /locus_tag="OO172_003267"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5513342.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHA"
BASE COUNT         3032 a         3091 c         3156 g         2873 t
ORIGIN      
        1 ggactcggtg aaatgaaaaa tccacgcaat tgcgtggatt ttatatactt ttgcgctctt
       61 catgagattt agcgaaacct catgagacaa taaattaatt agacgttgaa aaggaagttc
      121 atcacatcgc catctttcac gatgtaatct ttaccttctg cacgcatttt gcctgcttct
      181 ttcgcgcctt gttcaccttt gtaagtgatg aaatcttcaa acgagatggt ttgtgcacgg
      241 ataaagcctt tttcaaaatc agtatggatt ttgcccgctg cctgcggcgc ggttgctcca
      301 accggaatgg tccatgcacg cacttctttc accccagcgg tgaagtaagt ttgcaggttc
      361 agcagcttat aaccggcacg gatcacacgg ttcaggcccg gttcttccag cccaagctcc
      421 tgcataaact cgtcacgttc ttcgtcgtcc agttcggcaa tgtctgcttc aacagcagca
      481 caaaccggaa ccacaacaga accttctttc gccgcgattt cacgcacctg gtcaagatat
      541 gggttgtttt caaaaccgtc ttcgttgacg ttggctatgt acattgttgg tttcagcgtc
      601 aggaagctca ggtaacgaat agccgctttc tcttcagcgc ttaaatccag cgcgcgcagc
      661 atacctgcgt tttccagctg gggcaggcat ttttccagga ccgccagctc agctttcgcg
      721 tctttatcgc cacctttggc tttcttctgt acgcgatgaa tcgcacgttc gcaggtgtcg
      781 aggtctgcca gcgccagttc ggtgttgata acttcaatat cgtcagccgg gttaactttg
      841 ccggaaacgt gaatgatgtt gtcattttca aagcagcgaa caacgtgacc gatcgcttcg
      901 gtttcacgga tgttggtcag gaactggtta cccagacctt cgcctttcga tgcgcctttt
      961 accagaccgg cgatatcgac aaattccatg gtcgtgggaa gcgtacgttg cggttttacg
     1021 atttcagcca gttgatccag gcgagggtca ggcattggta cgacgcctgt gttcggctca
     1081 atggtgcaga atggaaagtt ggccgcttca ataccggctt tggtcagcgc gttgaacagg
     1141 gtagatttcc cgacgttggg caaaccgacg ataccgcatt tgaatcccat gatttaaatc
     1201 accttaatat cttaataatc aacctgttat tgctaacaga ttgcagaaat ggaaataact
     1261 ttgcctatta tacacggcac tcggcaaaaa tgccgcagac aacgacttat tgcgctttaa
     1321 aggcgtgcaa tcggttcgtt gctttggtca agccatctgt aaaccacatt tcagtacaac
     1381 gcgccgcttc gtcgatggct tcatcaatta acttctgttc actaacaggc ggtttgccta
     1441 acacaaaacc gacaacttta tttttatcgc ccggatgacc gattccgatg cgtaaacggt
     1501 gaaagttagg gttattaccc aatttactga tgatgtcttt cagtccattg tgaccaccat
     1561 ggccaccgcc caacttaaat ttggcgacgc caggaggcag atccagttcg tcgtgggcca
     1621 ccagaatttc gtccggatta atgcggaaaa aactggccat cgccgcaacg gctttgccgc
     1681 tgagattcat aaatgtagtc gggactaaca ggcggacatc ttcgcctcca agggtgactc
     1741 gcgaagtata accaaagaat ttagcctctt cgcgcagcgg agcgcgcaaa cgctctgcca
     1801 gtaagtcaac gaaccaggca ccagcattat gtcgcgttgc ggcgtattca gcaccggggt
     1861 tcgccaggcc gacaatcaat ttaatcgtca cgtttttttg tcctgagtgt gtacataact
     1921 ggcgcgtagt ttactggttg cggccccgct tgacaaaaaa ctgcgtatca aatgcagata
     1981 acgtaataat tgcctgagtg gactattaga aagtcaaggt gttcaggcgt ttatttgtaa
     2041 agttttgttg aaataagggt tgtaattgtg attacgcccg cacataaccc acagggtgtt
     2101 gtctatactt tacacataag gaagaggggt attccctgtt acaacccaga aagttccgga
     2161 ggtgacatat gaaacgcaaa aacgcttcga tactcggtaa cgtgctcatg gggttgggtc
     2221 tggtggtaat ggtggtcggc gtggggtatt caatcctcaa ccagttacca cagtttaata
     2281 tgccccagta tttcgcacat ggtgcagtgc taagtatttt cgtcggtgcc attctctggc
     2341 tggcgggtgc ccgtgttggc gggcatgaac aggtgtgcga ccgttactgg tgggttcgtc
     2401 actatgacaa acgttgccgc cgtagcgata atcgccgtca tagctaacaa ataatgcagt
     2461 ttgctgacca gtcagtttgc gctgactggt caatctcgta cttataaatc cgccatcgcc
     2521 gcgcgacgat tcgggaagaa cgcgaggcgt cccgggatcg gttgaatgcc tgcgcgagcc
     2581 atagttcgca gtggctggaa ttccacgttg cacacgcgca gttcacatcc ttcgggcaga
     2641 cgcttcacaa aacgctggaa cgcatcaaga ccaccagcat caagtaccgg aacggcatcc
     2701 cacttcagaa tcacaatccg tttgccttca agacgtgact ccaggtccgt gaataagcct
     2761 tcagcagcag caaaaaacag cgggccaata acgcgcagaa ccaggacatc gtctggaaca
     2821 tctacgacta ccggtgccag gcgagtcata cgtgcgatac gacgcataaa cagcagcgat
     2881 gccagcacga tccccacgct gatggcaata accatatcaa acagcacggt cagtgacata
     2941 cacagcagca tgacgatgat gtcatctttc ggcgcatgac gcagcaagtc gaccactttg
     3001 tgcgcttcac tcatgttcca cgccaccatc aacagcaggg ctgccatggc ggaaagcggc
     3061 agccaggaga gcagtggtgc cagtaccagc agggcgagaa taaccagaat agagtggatc
     3121 accgccgaga taggggaagt tgccccggca cggacgttag cggcagaacg cgcgatggca
     3181 gcagtagcgg taataccacc aaagaatgga gcgatgatat tccccagccc ctgaccgacc
     3241 agttcactat tcgccttgtg cttagtcccg gtcataccat ccagtaccac agcgcagagc
     3301 agagattcga tcgccccgag cattgccatt gagaatgccg caggcagcag ggtgcgaata
     3361 gaatcccagg ttagcgtgaa ttctgaatta ggcagatccc acggcagcac cagttgcggc
     3421 agcagttgcg gaataccgtt accctgagaa ccatcggcca ggacgtagtg gaattgcgaa
     3481 ccgatggtag caacatgtcc gccgagcagg ttaacaatcc ccatcaccgc gcaaccagcc
     3541 agcaaggccg gaaggtgacc gggtaaacga atgcccagac gcggccagaa aaccagaatg
     3601 ccaagcgtca caatgccaat ggcagcatca cccacattaa tggtcggcag cgccataaat
     3661 aatgcgccga ctttttgtag ataatgttcc gggacatggg ccatttgcag accgagaaaa
     3721 tctttaatct gcatggtacc gatggtgatc ccgatacccg aggtgaaacc taaggtgacg
     3781 gaaaccggaa tatactcaat cagacgacca aagcgtgcca gccccatcag aatcaaaaag
     3841 attcccgaca acaaggtcgc aaccagcagt cctgccagtc caaactgttg cgaaacggga
     3901 tagagaatta ccacaaatgc cgcagtcgga ccggaaacgc taaagcgtga cccacccgtt
     3961 agagcaatga caatccccgc aacagctgcg gtatataaac cgtactgggg tgccacacca
     4021 ctaccaatag ccaacgccat cgccagcggg atagcaataa tcccgacggt tatcccggca
     4081 atcaggtcac gggtaaaccg tgcggcagta tatttttctt tccagcaagc gtcgatcaga
     4141 gcgcggaaag gcatcacatg tgaggaaaat attttgttca caataatgtt tcatccgtga
     4201 gcgcatcatc tgtcaactaa atggcaggtg aaggaggcat aggtcataca aatggatatt
     4261 acagacaaaa aaacccgccg cagcgggtct ttgagccggg ttcgattagt gttcgaacat
     4321 ggcagagatc gattcttcgt tgctgatacg acgaatcgct tcggccagca tacctgacag
     4381 ggtcagagta cgcacgttcg gcagtgattt gatttcatcg ctcagcggaa tggtatcgca
     4441 gacaacgact tcatcaatta cagagttacg caggttgttc gccgcgttgc cagagaagat
     4501 cgggtgagtc gcgtacgcaa atacacgttt agcaccacgt tctttcagcg cttcagcagc
     4561 tttacacagc gtaccgccag tgtcgatcat atcatcgacc agtacgcagt cacgacctgc
     4621 aacgtcaccg atgatatgca tcacctggga aacgttcgca cgcggacgac gtttgtcgat
     4681 gattgccata tcggtatcgt tcagcagctt agcgatagcg cgggcacgca caacgccgcc
     4741 gatgtccgga gaaaccacaa ttgggttatc cagattcagc tgcagcatgt cttccagcag
     4801 gatcgggcta ccaaatacgt tatcaaccgg aacgtcgaag aaaccctgaa tctgttcagc
     4861 gtgcagatcc actgtcagca cacggtcaac accgacgctg gagaggaagt ctgcaaccac
     4921 tttcgcagtg attggtacac gagcggaacg gacgcgacgg tcctggcgcg catagccaaa
     4981 gtaggggata acagcggtga tacgacctgc ggaagcacga cgcagggcat caaccataac
     5041 gactaattcc atcaggttgt cgttagtagg ggcacaagtg gactggatga tgaaaatatc
     5101 accaccgcgt acattttcgt taatttgtac gctgacttcg ccgtcgctaa agcgacctac
     5161 agcggcgtcg ccgagtgaag tgtacaggcg gttggcaata cgttgtgcta gtaccggggt
     5221 ggcgttacca gcaaaaagct tcatatcagg cacgagaaga acctcaggca tgcgtccatt
     5281 ggtggaaaga atctgccgaa aactgtgcgg gccaggcatg atcctttcca ggcggtgtat
     5341 taaagagcgc gatgcaacgt ctggaacaag gtgacgttgt caccgaaact cagcttgccc
     5401 ggcttaaagc atggctctgt gcaatgggga aagattaacg cctttcgcca caaagccatt
     5461 gagccattcc ggggcttgct ctagcacctg gcgggcttca gactctgtat caaattcagc
     5521 aaagacacag gcccctgtcc cagtcaggcg cgacggggcg tattttaaca gccaggaaag
     5581 caccgcatca acctcgcgaa aacgttttct tgcgataacc tcgcaatcat tgctgaattc
     5641 acattttagc aacgtttcta ttgacctttt tggcgtattg cgcgggagtt caggatcttt
     5701 aaaaatcacc ggagtcggaa tacttacacc agggtgcgcc accagatacc acttctctgg
     5761 cggatccacc ggcgttagta tttcaccaac gccttcggca aacgcggcat gcccccgaac
     5821 aaagacagga acatctgcgc ccagcgtcag ccccatttcc gccagctcat ccatgcttag
     5881 cccgcattgc cagagatgat ttaatgccac caggaccgtc gcggcattgg atgaaccacc
     5941 gccgagaccg ccgcccatcg gcaaacgctt gtcaatgctg atattcgcac cgcttcccgt
     6001 cggaagacgc ccgctgtctg ccgcagtttt catcaacaac cgcgctgcgc gaacgatcag
     6061 gttatcttca tgttccacgc cttcaacggg cgttaacaga cgaatatccc catcgtcacg
     6121 aagctcaatg ctgatggtgt cgccgtaatc aagaaactga aacagcgttt gcagcgtgtg
     6181 gtaaccatcc gcacgctgac cggtaatgta taaaaacaga ttaagttttg ccggagaggg
     6241 ccactgtgtc cgcattattt cactatccag ttatccattt ttaacttgat gcgttgacca
     6301 ccgtcggtga gttccatatt ggttggcatc gcaggttgcg ttttggtgtc ataaccacca
     6361 taaacaacct tccagttttt gccattctgg ctgtaggtaa tttcgctcag gcggtactgg
     6421 tcgtccagtt tgtagtcggt tgcatcaccc ggtaaaccta aaatccactg gcgcaagctg
     6481 ttgagcggaa ttggcattcc ggtcaatttg ccaatcatct cttcggcgtc atcggcggta
     6541 taacgctgac ctttattgtc gactaactgc acgttacccg gttgagcatt cagctccagt
     6601 tccgtgctgc ccaatgggtt agtgagcagc agacggtagc gatcctggcc ggtttgctgc
     6661 cagaaaaagc gggcgtacac tttttgttgg tcagaaatat aagcgaacgc gccgcgagtc
     6721 tgatactgat taagattgcg cacgtcttgc tgatgctgac gccattgtgg cgaatccggg
     6781 cttttgccag gacctttggg cgtggtaacg gaacaggcag tgagcacaag agcagccagc
     6841 ggtagcaggc ggataagacg aaaatcgggc aggggcatag tgatgacaag tccttgagat
     6901 acgttgcagt tataaccctt aatgctagcg ttaccgtccg ctatcgtcta tgttcaagtt
     6961 gtcttaattg ccagaatcta acggctttcg gcaattactc caaaaggggg cgctctcttt
     7021 tattgatctt acgcatcctg tatgatgcaa gcagactaac cctatcaacg ttggtattat
     7081 ttcccgcaga catgaccctt ttagcactcg gtatcaacca taaaacggca cctgtatcgc
     7141 tgcgagaacg tgtatcgttt tcgccggata agctcgatca ggcgcttgac agcctgcttg
     7201 cgcagccgat ggtgcagggc ggcgtggtgc tgtcgacgtg caaccgcacg gaactttatc
     7261 ttagcgttga agagcaggat aacctgcaag aggcgttaat ccgctggctt tgcgattatc
     7321 acaatcttaa tgaagaagat ctgcgtaaaa acctctactg gcatcaggat aacgacgcgg
     7381 ttagccattt aatgcgtgtt gccagcggcc tggattcact ggttcttggg gagccgcaga
     7441 tcctcggtca ggttaaaaaa gcgtttgccg attcgcaaaa aggtcatatg aaggccagcg
     7501 aactggaacg catgttccag aaatctttct ctgtagcgaa acgcgttcgc actgaaacag
     7561 atatcggtgc cagtgctgtg tctgtcgctt ttgcggcttg tacgctggcg cggcagatct
     7621 ttgaatcgct ctctacggtc acagtgttgc tggtaggcgc gggcgaaacc atcgagctgg
     7681 tagcgcgtca tctgcgcgaa cataaagtac agaagatgat tatcgccaac cgcactcgcg
     7741 aacgtgccca aattctggca gatgaagttg gcgcggaagt gattgccctg agtgagatcg
     7801 acgaacgtct gcgcgaagcc gatatcatca tcagttccac cgccagcccg ttaccgatta
     7861 tcgggaaagg catggtggag cgcgcattaa aaagtcgtcg taaccaacca atgctgttgg
     7921 tggatattgc cgttccgcgc gatgttgagc cggaagttgg caaactggcg aatgcttatc
     7981 tttatagtgt ggacgatctg caaagcatca tttcgcacaa cctggcgcag cgtaaagccg
     8041 cagcggttga ggcggaaact attgtcgctc aggaaaccag cgaatttatg gcgtggctgc
     8101 gagcacaaag cgccagcgaa accattcgcg agtatcgcag ccaggcagag cacgttcgcg
     8161 atgagttaac cgccaaagcg ttagcggccc ttgagcaggg cggcgacgcg caagccatta
     8221 tgcaggatct ggcatggaaa ctgactaacc gcttgatcca tgcgccaacg aaatcacttc
     8281 aacaggccgc ccgtgacggg gataacgaac gcctgaatat tctgcgcgac agcctcgggc
     8341 tggagtagca gtacatcatt ttcttttttt acagggtgca tttacgccta tgaagccttc
     8401 tatcgttgcc aaactggaag ccctgcatga acgccatgaa gaagttcagg cgttgctggg
     8461 tgacgcgcaa actatcgccg accaggaacg ttttcgcgca ttatcacgcg aatatgcgca
     8521 gttaagtgat gtttcgcgct gttttaccga ctggcaacag gttcaggaag atatcgaaac
     8581 cgcacagatg atgctcgacg atcctgaaat gcgtgagatg gcgcaggatg aactgcgcga
     8641 agctaaagaa aaaagcgagc aactggaaca gcaattacag gttctattac taccaaaaga
     8701 tcctgatgac gaacgtaacg ccttcctcga agtccgtgcc ggaaccggcg gcgacgaagc
     8761 agcgctgttt gcaggcgatc tgttccgtat gtacagccgt tacgccgaag cccgccgctg
     8821 gcgggtagaa atcatgagcg ccagcgaggg tgaacatggt ggttataaag agatcatcgc
     8881 caaaattagc ggtgatggtg tgtatggtcg tctgaaattt gaatccggcg gtcatcgcgt
     8941 gcaacgtgtt cctgctacgg aatcgcaggg tcgtattcat acttctgctt gtaccgttgc
     9001 ggtaatgcca gaactgcctg acgcagaact gccggacatc aacccagcag atttacgcat
     9061 tgatactttc cgctcgtcag gggcgggtgg tcagcacgtt aacaccaccg attcggcaat
     9121 tcgtattact cacttgccga ccgggattgt tgttgaatgt caggacgaac gttcacaaca
     9181 taaaaacaaa gcgaaggcgc tttcagtgct cggtgctcgc atccacgctg ctgaaatggc
     9241 aaaacgccaa caggccgaag cgtctacccg tcgtaacctg ctggggagtg gcgatcgcag
     9301 cgaccgtaac cgtacttaca atttcccgca ggggcgcgtt accgatcacc gcatcaacct
     9361 gacgctctac cgcctggatg aagtgatgga aggtaagctg gatatgctga ttgaaccgat
     9421 tatccaggaa catcaggccg accaactggc ggcgttgtcc gagcaggaat aatggaatat
     9481 caacactggt tacgtgaagc aataagccaa cttcaggcga gcgaaagccc gcggcgtgat
     9541 gctgaaatcc tgctggagca tgttaccggc agagggcgta cttttattct cgcctttggt
     9601 gaaacgcagc tgactgacga acaatgtcag caacttgatg cgctactgac acgtcgtcgc
     9661 gatggtgaac ccattgctca tttaaccggg gtgcgagaat tctggtcgtt gccgttattt
     9721 gtttctccag cgaccttaat tccgcgcccg gatacggagt gtctggtgga gcaggcactg
     9781 gcgcggttgc ctgaacaacc ttgccgtatt ctcgatctcg ggacggggac cggggcgatt
     9841 gcgcttgcgc tggctagcga gcgcccggac tgcgaaatta tcgctgtaga tcgtatgcct
     9901 gatgctgtct ccctggcaca acgtaatgcc cagcatctgg cgatcaaaaa tatccacatt
     9961 ctgcaaagcg actggtttag cgcgctagcc gggcagcagt ttgcgatgat tgtcagcaat
    10021 ccgccgtata ttgacgagca ggacccacat cttcaacaag gcgatgtccg ctttgagccg
    10081 ctcactgcgc tggttgcggc agacagtgga atggcagaca tcgtgcatat catcgaacag
    10141 tcgcgtaacg cgctggtatc cggcggcttt ctgcttctgg aacatggctg gcagcagggc
    10201 gaagcggtgc gacaagcatt tatcctcgcg gggtatcatg acgtcgaaac ctgccgtgac
    10261 tatggtgata acgagcgcgt aacgctcggc cgctattatc aatgacaagt ttttctacac
    10321 tgcttagtgt tcatcttatt agtatcgcgc tttctgttgg gctattaacc ttacgtttct
    10381 ggctacgtta tcagaagcat ccacaggcat ttgctcgctg gacgcgcatt gtgccgccgg
    10441 ttgtcgatac ggtgttattg ttaagcggca ttgcgttgat ggctaaagcg cacatcctgc
    10501 cattttccgg gcaggcacag tggctgactg aaaagctgtt tggagttatc atttatatcg
    10561 ttttgggttt tattgcactc gattatcgtc gtatgcacag tcagcaggcg cgcattattg
    10621 ccttcccgct ggcgttggtg gtgctgtaca tcatcattaa actcgccacc acaaaagtac
    10681 cgttactggg gtaagttatg agatcgttag ctgatttcga atttaataaa gcgccattgt
    10741 gcgaaggcat gatcctggct tgcgaagcaa tccgccgcga ttttccctcg caagatgttt
    10801 acgacgaact ggagcgtctc gttagtctgg cgaaggaaga aatcagccag cttctgcctt
    10861 tagaagagca gctggaaaaa ctgatcgcgc tgttttacgg cgactgggga tttaaagcct
    10921 cacgcggcgt ttatcgtctt tccgatgcat tatggctgga ccaggtgtta aagaatcgac
    10981 agggcagtgc ggtatcatta ggtgcggttt tattatgggt cgcgaatcgt ctcgatttgc
    11041 cgctgctgcc ggtgattttc cctacgcagc tgatattgcg cattgaatgt ccggatggcg
    11101 aaatttggct gattaatcct tttaacggtg aatcgttaag cgaacatatg ctggacgtat
    11161 ggttaaaggg aaatatcagc ccgtcggcgg aactgtttta tgaagacctt gatgaagctg
    11221 ataacattga ggtaatccgc aaattgctgg atacactcaa agcctcgttg atggaagaaa
    11281 atcagatgga gctggcgtta cgcaccagcg aagctttatt acaattcaac cctgaagatc
    11341 cctatgaaat tcgcgatcgc gggttgattt atgcgcaact ggattgcgaa cacgttgcgt
    11401 tgaacgattt aagttatttc gttgaacagt gtccggaaga cccgatcagc gaaatgatcc
    11461 gtgcgcaaat aaataacatc gcgcataaac atattgtgct gcattaatta atcgacattt
    11521 tactcaagat taaggcgatc ctatgaaaca aaaagtggtt agcattggcg acatcaacgt
    11581 agcaaatgac ctgccgttcg tactgtttgg cggtatgaac gtgctggaat ctcgcgatct
    11641 ggcgatgcgc atttgcgagc actacgtaac tgtgacccag aaactgggta tcccttacgt
    11701 gttcaaagcc tcttttgaca aagccaaccg ctcctccatc cactcttatc gtggaccggg
    11761 cctggaagaa gggatgaaaa tcttccagga gttgaagcag acttttggcg tgaaaattat
    11821 caccgacgtt cacgaaccaa gtcaggcaca gcccgttgct gatgtcgtgg atgtgattca
    11881 gttgccggcg tttcttgctc gccagactga cctggttgaa gccatggcga aaaccggtgc
    11941 ggtaattaac gtcaagaaac cacagtttgt cagcccgggg cagatgggta atatcgttga
    12001 taaattcaaa gaaggcggca acgaaaaagt gattctttgc gatcgtggtg ctaacttcgg
    12061 ctatgacaac ctggttgtcg atatgctggg cttcagcatt atgaagaaag tgtctggtaa
    12121 ctcgccggtg attttcgacg tgacccacgc ac
//
LOCUS       DAJTNM010000115        12143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTNM010000115 DAJTNM010000000
VERSION     DAJTNM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.115"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            117..986
                     /locus_tag="OO172_003268"
     CDS             117..986
                     /locus_tag="OO172_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5513343.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALPLKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYARTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1189..1542)
                     /locus_tag="OO172_003269"
     CDS             complement(1189..1542)
                     /locus_tag="OO172_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5513344.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1680..3326)
                     /gene="groL"
                     /locus_tag="OO172_003270"
     CDS             complement(1680..3326)
                     /gene="groL"
                     /locus_tag="OO172_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5513345.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3370..3663)
                     /locus_tag="OO172_003271"
     CDS             complement(3370..3663)
                     /locus_tag="OO172_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5513346.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3939..5195
                     /gene="yjeH"
                     /locus_tag="OO172_003272"
     CDS             3939..5195
                     /gene="yjeH"
                     /locus_tag="OO172_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5513347.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5211..5687)
                     /gene="fxsA"
                     /locus_tag="OO172_003273"
     CDS             complement(5211..5687)
                     /gene="fxsA"
                     /locus_tag="OO172_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5513348.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6024..7460
                     /gene="aspA"
                     /locus_tag="OO172_003274"
     CDS             6024..7460
                     /gene="aspA"
                     /locus_tag="OO172_003274"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5513349.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7578..8879
                     /gene="dcuA"
                     /locus_tag="OO172_003275"
     CDS             7578..8879
                     /gene="dcuA"
                     /locus_tag="OO172_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5513350.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8995..9333
                     /gene="cutA"
                     /locus_tag="OO172_003276"
     CDS             8995..9333
                     /gene="cutA"
                     /locus_tag="OO172_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5513351.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9309..11006
                     /gene="dsbD"
                     /locus_tag="OO172_003277"
     CDS             9309..11006
                     /gene="dsbD"
                     /locus_tag="OO172_003277"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5513352.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11043..11618
                     /locus_tag="OO172_003278"
     CDS             11043..11618
                     /locus_tag="OO172_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5513353.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11725..11800
                     /locus_tag="OO172_003279"
     tRNA            11725..11800
                     /locus_tag="OO172_003279"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11758..11760,aa:Phe,seq:gaa)
BASE COUNT         2925 a         3136 c         2986 g         3096 t
ORIGIN      
        1 tatagagtga gcacggctat ttatgttatt ttaacaacta ttactaccaa gcccatttat
       61 ctggcttata aattaaaatt aattatttaa ttgagaaatg gttagggaga acctacatgg
      121 ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat ttcattgcac
      181 tgccgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg tctgtcatgg
      241 aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat aaactggatg
      301 ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa atggcagaaa
      361 acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt cgggttaaca
      421 cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg ctacatgacg
      481 gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg cgagcaatca
      541 ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc tcactgttag
      601 atttcctgcc gttatacgac gttgactgcc aggacaatgg caatctggaa tatgacacct
      661 attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg ctttaccgct
      721 ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg cgtgaagcaa
      781 cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc ccacgactga
      841 aaaaactcgc gggtgtgcca tgtcaggtct atgctcgcac cgtagcggcg aataacgccc
      901 aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgta caatccacca
      961 gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct ggagccatcc
     1021 agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta aacgccttat
     1081 ccggcctaca aattctcaag cattcaatca gttgcaatgc ctgctgtagg cctgataagc
     1141 gaagcgcatc aggcaataat acgtttgtca cccagcctct ggatcacatc agttcgggca
     1201 cttataaacc tgaccgataa tctggctatc cgtcgggaca aaactggaca acattccctg
     1261 cgacgggctg ctgatgccat aaatcacgtt accgcccatt gcagccgcct ggttgcgcag
     1321 atcgtttgct gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg aaagccagtt
     1381 gctttgctta cctgtcgcag taccaatcag ctggcactct gcgcctggct gctcgtccac
     1441 aatgcgtaca ctctgaccgg cagcactcaa ttcgttgctg gagctacaac ccgccatcag
     1501 tagcgcggca ccgacaatcc ctgctaagta ttttacgtgc atgttattcc ccataatcaa
     1561 tgagctggac gccactcgcg tcgtccgtgt ctgaatctta tactagaaag atgaccaaaa
     1621 gaaaaacccc cagacatttc tgcccggggg tttgtttatt tctgcgaagt gcagggcaat
     1681 tacatcatgc cgcccatgcc acccatgccg cccataccgc cagcagcgcc taagtcagct
     1741 gcatcgtttt tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag gccagccaca
     1801 gaagctgcgt actgcagagc agaacgggtt actttggttg ggtccaggat acccatgtcg
     1861 atcatgttgc cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc gccgccttta
     1921 acggtgttag caacaacaga cggttcttcg ccgcagttca ggacgatctg acgcagcgga
     1981 gcttccattg cacgcagtgc aactttgata cccacgttct ggtcttcgtt ctgaccacgc
     2041 aggtcagcca gtttagacgc tacgcggatc agcgcaacac caccaccagc aaccacgcct
     2101 tcttctaccg cagcacgggt cgcgtgcagg gcatcttcaa cgcgtgcttt tttctctttc
     2161 atttcaactt cggtagcagc acctactttg ataactgcaa cgccgcctgc cagtttcgct
     2221 acgcgctcct gcagtttttc acggtcgtag tcagaagttg cttcttcaat ctgctgacgg
     2281 atctgagcaa cacggccctg gattgcagct tcttcaccca cgccatcgat gatagtggtg
     2341 gtgtctttgt tgatcacaac acgtttagcc tgacccaggt cttccagggt tgctttttcc
     2401 agctccatac cgatctcttc agagattacg gtaccgccag tcagggttgc gatatcctgc
     2461 agcatagctt tacgacgatc gccgaagccc ggagctttaa ccgcagcaac tttcacgatg
     2521 ccacgcatgg tgttaacaac cagagttgct agcgcttcgc cttcaacatc ttcagcgatg
     2581 atcagcagcg gtttgcctgc tttggcaaca gcttccagaa ccggcagcat ttcgcggatg
     2641 ttggagattt tcttgtcagc cagcaggatg aacgggcttt ccagttctac tgcgccagtt
     2701 tccggcttgt tgatgaagta aggagacagg tagccacggt cgaactgcat accttcaacc
     2761 acgtccagtt cgtcctgcag accggtaccg tcttcaacgg tgataacgcc ttctttaccg
     2821 actttgtcca tcgcttcagc gatcagttta cctacggttt cgtcggagtt agcggagatg
     2881 gtaccaacct gagcaatcgc tttagaatca gagcacggta cggacagcgc tttcagttct
     2941 tcaactgcag cggtaaccgc tttgtcgata ccacgtttca ggtccatcgg gttcatgccc
     3001 gcagcaacag ctttcagacc ttcagtgatg atagcctgag ccagtacggt tgcagtggtg
     3061 gtaccgtcgc ctgcagcgtc gttcgctttg gaggcaactt ctttcaccat ctgcgcaccc
     3121 atattttcga acttgtcttc cagttcgatt tcacgagcaa cggaaacacc atctttggtg
     3181 atggtcggtg caccgaaaga tttatccaga actacgttac ggccttttgg accgagggta
     3241 actttcactg catctgccag tacgtttacg ccgcgcagca ttttcacacg agcgtcgtta
     3301 ccgaatttta cgtctttagc tgccattatc tttattcctt aaattcgtat gttcagtgtc
     3361 gtgcgcggat tacgcttcaa caattgccag aatgtcgctt tcggacatga tcaacacttc
     3421 ttcattgtcg atcttctcgg atttcacacc gtagccatcg ttgaaaataa cgatgtcgcc
     3481 aactttcacg tccagcggct tcacttcgcc attttcaagg atacggccat tgccgacagc
     3541 cagcacttcg ccgcgggtgg atttagccgc tgcagagccg gtcagaacga tgccgccagc
     3601 agatttagtt tcaacttctt tacgcttgac gatcacgcga tcatgcaatg gacgaatatt
     3661 cattgataac tctcctttga gaaagtccgt atctgttatg ggtgacgccg gagcttacgt
     3721 ggtttcccgg ctggtgacca gagaaatggg gatgaggctt cgccccttca agggggaaaa
     3781 agaaaaaaaa ttctgaacag cacaaaatcg ggtgaaaacc ctgattcacc tcacatttca
     3841 tcgcaatttc ttcatcgccg tcataagcga atctgattgt gctaccatcg aaaatctacg
     3901 cagttgccca aaatttgggc gcaatcggat atcaacccat gagtggactc aaacaagaac
     3961 tggggctggc ccagggcatt ggcctgctat cgacgtcatt attaggcact ggcgtgtttg
     4021 ccgttcctgc gttagctgca ctggtagcag gcaataacag cctgtgggcg tggcccgttt
     4081 tgattatctt agtgttcccg attgcgattg tgtttgcgat tctgggtcgc cactatccca
     4141 gcgcaggcgg cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt gagcgagtca
     4201 ccggctggct gtttttatcg gtcattcccg tgggtttgcc tgccgcacta caaattgccg
     4261 ccggattcgg ccaggcaatg tttggctggc atagctggca actgttgttg gcagaactcg
     4321 gtacgctggc gttggtgtgg tatatcggta ctctcggtgc cagttccagt gctaatctac
     4381 aaaccgttat tgccggactt atcgtcgcac tgattgtcgc tatctggtgg gcgggcgata
     4441 tcaaacctgc gagtatcccc ttccccgcgc caggaaatat cgaacttacc gggttatttg
     4501 ctgcgttatc agtgatgttc tggtgttttg tcggtctgga agcatttgcc catcttgcct
     4561 cggaatttaa aaatccagag cgtgattttc ctcgtgcttt gatgattggt ctgctgctgg
     4621 caggattagt ctactggggc tgtacggtag tcgtcttaca cttcgacgcc tatggtgaac
     4681 aaatggcggc ggcagcatcg cttccaaaaa ttgtagtgca gttgttcggt gtaggagcgt
     4741 tatggattgc ctgcgtaatt ggctatctgg cctgctttgc cagtcttaac atttatatac
     4801 agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac tacctggcac
     4861 gcctctcttc tcgccatatc ccgaataatg ccctcaatgc ggtgcttggc tgctgcgtgg
     4921 tgagcacgtt ggtaattcat gccttagaga tcaatctgga ggctcttatt atttatgcca
     4981 atggcatctt tattatgatt tatctgttat gcatgctggc aggctgtaaa ttattgcaag
     5041 gtcgttatcg actactggcg gtggttggcg ggctgttatg cgttctgtta ctggcaatgg
     5101 tcggctggaa aagtctctat gcgctgatca tgctggtggg gttatggctg tttctgccaa
     5161 aacgaaaaac gccggaaaat ggcataacca cataatccgg cgtttcgaca ttaatcctgg
     5221 cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct ttcgctgata ctcaccatca
     5281 aaagtattac cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg agaaaagcgc
     5341 aaatgtggca tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa aagcagaaga
     5401 ccgaggaagt cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat aatcagcgaa
     5461 acacttttaa tcatctccgc cgcagggttt tcacccgccg ccattttttg ctgcatcagc
     5521 acgaaattct taaagccctg gttacgtacc agtgacatac cgataactga cgtgaatata
     5581 accagcacga gggtcagcaa tacccccaat acatgggcaa cctgaataaa gattgaaatc
     5641 tcaatataga cataaaggaa aatggcaata aaaggtaacc agcgcaaagg tttctcctgt
     5701 aatagcagcc ggttaaaccc ggctacctga atgggttgcg aatcgcgttc agcttatatt
     5761 gtggtcatta gcaaaatttc aagatgtttg cgcaactatt tttggtagta atcccaaagc
     5821 ggtgatctat ttcacaaatt aataattaag gggtaaaaac cgacacttaa agtgatccag
     5881 attacggtag aaatcctcaa gcagcatatg atctcgggta ttcggtcgat gcaggggata
     5941 atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag caaatcattg
     6001 gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa gatctgttgg
     6061 gtaccaggga agttccagct gatgcctact atggtgttca cactctgaga gcgattgaaa
     6121 acttctatat cagcaacaac aaaatcagtg atattcctga atttgttcgc ggtatggtaa
     6181 tggttaaaaa agccgcagct atggcaaaca aagagctgca aaccattcct aaaagtgtag
     6241 cgaatgccat cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc atggatcagt
     6301 tcccggtaga cgtctaccag ggcggcgcag gtacttccgt aaacatgaac accaacgaag
     6361 tgctggccaa tatcggtctg gaactgatgg gtcaccagaa aggtgaatat cagtacctga
     6421 acccgaacga ccatgttaac aaatgtcagt ccactaacga cgcctacccg accggtttcc
     6481 gtatcgcagt ttactcttcc ctgattaagc tggtagatgc gattaaccaa ctgcgtgaag
     6541 gctttgaacg taaggctgtc gaattccagg acatcctgaa aatgggtcgt acccagctgc
     6601 aggacgcagt accgatgacc ctcggtcagg aattccgcgc tttcagcatc ctgctgaaag
     6661 aagaagtgaa aaacatccaa cgtaccgctg aactgctgct ggaagttaac cttggcgcaa
     6721 cagcaatcgg tactggtctg aacacgccga aagagtactc tccgctggca gtgaaaaaac
     6781 tggctgaagt cactggcttc ccatgcgtac cggctgaaga cctgatcgaa gcgacctctg
     6841 attgcggcgc ttatgttatg gttcacggcg cgctgaaacg cctggctgtg aagatgtcca
     6901 aaatctgtaa cgacctgcgc ttgctctctt ctggtccacg tgccggcctg aacgagatca
     6961 acctgccgga actgcaggcg ggctcttcca tcatgccagc taaagtaaac ccggttgttc
     7021 cggaagtggt taaccaggta tgcttcaaag tcatcggtaa cgacaccact gttaccatgg
     7081 cagcagaagc aggtcagctg cagttgaacg ttatggaacc ggtcattggc caggccatgt
     7141 tcgaatccgt tcacattctg accaacgctt gctacaacct gctggaaaaa tgcattaacg
     7201 gcatcactgc taacaaagaa gtgtgcgaag gttacgttta caactctatc ggtatcgtta
     7261 cttacctgaa cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa atctgtgccg
     7321 aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact gaagcggaac
     7381 ttgacgatat tttctccgta cagaatctga tgcacccggc ttacaaagca aaacgctata
     7441 ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca cgtcagatga
     7501 cgtgcctttt ttcttgtgag cagtaactta aaaataacaa tctaatatca acttgttaaa
     7561 aaacaaggaa ggctaatatg ctagttgtag aactcatcat agttttgctg gcgattttct
     7621 tgggcgccag attgggggga ataggtattg gttttgcagg cggattgggg gtgctggttc
     7681 ttgccgctat tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc attatcatgg
     7741 cggttatcgc cgctatttct gccatgcagg ttgctggcgg tctggactat ctggttcatc
     7801 agacagaaaa gctgctgcgc cgtaacccga aatacatcac gatcctcgca ccgatcgtga
     7861 cctatttcct gactatcttt gctggtactg gcaacatctc tctggcgaca ctgccagtta
     7921 tcgctgaagt tgcgaaggaa caaggcgtta aaccttgccg tccgctctct actgcagtgg
     7981 tatccgcgca gattgcgatc accgcatcgc caatctcagc ggcagtggtt tacatgtctt
     8041 ccgtgatgga aggtcatggc atcagctacc tccatctgct ctccgtggtc atcccgtcca
     8101 ccctgctggc ggttctggtg atgtccttcc tggtcactat gctgttcaac tccaaactct
     8161 ctgacgatcc gatttatcgc aagcgtctgg aagagggctt ggttgaactg cgcggtgaaa
     8221 agcagattga aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg ctgggcgtag
     8281 ttggcgtggt tatctatgca atcatcaaca gcccaagcat gggtctggtt gaaaaaccgc
     8341 tgatgaacac caccaacgca atcctgatca tcatgctcag cgttgcaact ctgaccaccg
     8401 ttatctgtaa agtcgatacc gacaacatcc tcaactccag caccttcaaa gcaggtatga
     8461 gcgcctgtat ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt tccaacaaca
     8521 tcgactggat caaagatacc gctggtgaag tgattcaggg tcatccgtgg ctgctggccg
     8581 tcatcttctt ctttgcttct gctctgctgt actctcaggc tgcaaccgca aaagcactga
     8641 tgccgatggc tctggcactg aacgtttcac cgctgaccgc tgttgcttct ttcgctgcgg
     8701 tgtctggtct gttcattctg ccgacctacc cgacgctggt tgctgcggta cagatggatg
     8761 acacgggtac tacccgtatc ggtaaattcg tcttcaacca tccgttcttc atcccgggta
     8821 ctctgggtgt tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc atgctgtaat
     8881 gatcaatcgc ggggcgttca cgccccgctt tctttcccgc cgactaacat ccttcccccg
     8941 tccgttgtat agtgacctct ctcttgcggt tccatctgtt cttgcgaggt gtttatgctt
     9001 gatgaaaaaa gttcgaatac cgcgtctgtc gtggtgctat gtaccgcacc ggatgaagcg
     9061 acagcccagg atttagccgc caaagtgctg gcggaaaaac tggcggcctg cgcgaccttg
     9121 atccccggcg ctacctctct ctattactgg gaaggtaagc tggagcaaga atacgaagtg
     9181 cagatgattt taaaaactac cgtatctcac cagcaggcac tgctggaatg cctgaagtct
     9241 catcatccat atcaaacccc ggaacttctg gttttacctg ttacacacgg agacacagat
     9301 tacctctcat ggctcaacgc atctttacgc tgattctgct actttgcagc acttccgttt
     9361 ttgccggatt attcgacgcg ccgggacgtt cacaatttgt ccccgcggat caagcctttg
     9421 cttttgattt tcagcaaaac caacatgacc ttaatctgac ctggcagatc aaagacggtt
     9481 actacctcta ccgtaaacag atccgcatta cgccggaaca cgcgaaaatt gccgacgtgc
     9541 agctgccgca aggcgtctgg catgaagatg agttttacgg caaaagcgag atttaccgcg
     9601 atcggctgac gcttcccgta accatcaacc aggcgagtgc gggagcgacg ttaactgtca
     9661 cctaccaggg ctgtgctgat gccggtttct gttatccgcc agaaaccaaa accgttccgt
     9721 taagcgaagt ggtcgccaac aacgcagcgt cacagcctgt gtctgtttcg cagcaagagc
     9781 agcacaccgc gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt attggtatcg
     9841 cctttacgcc atgcgtgctg ccaatgtacc cactgatttc tggcatcgtg ctgggtggta
     9901 aacagcggct ctccactgcc agagcattgt tgctgacctt tatttatgtg caggggatgg
     9961 cactgactta cacggcgctg ggtctggtag ttgccgccgc agggttacag ttccaggcgg
    10021 cgctacagca cccatacgtg ctcattggcc tcgccatcgt ctttaccttg ctggcgatgt
    10081 caatgtttgg cttgtttact ctacaactcc cctcttcgct gcaaacacgt ctcacgttga
    10141 tgagcaatcg ccaacagggc ggctcacctg gcggtgtgtt tgttatgggg gcgattgccg
    10201 gactgatctg ttcaccatgc accaccgcac cgcttagcgc gattctgctg tatatcgccc
    10261 aaagcgggaa catgtggctg ggcggcggca cgctttatct ctatgcgttg ggcatgggcc
    10321 tgccgctgat gctaattacc gtctttggta accgcttgct gccgaaaagc ggcccgtgga
    10381 tggaacaagt caaaaccgcg tttggttttg tgatcctcgc actgccggtc ttcctgctgg
    10441 agcgagtgat tggtgatgta tggggattac gcttgtggtc ggcgctgggt gtcgcattct
    10501 ttggctgggc ctttatcacc agcctacagg ctaaacgcgg ctggatgcgc gtggtgcaaa
    10561 taatcctgct ggcagcggca ttggttagcg tgcgcccact tcaggattgg gcatttggtg
    10621 cgacgcatac cgcgcaaact cagacgcatc tcaactttac acaaatcaaa acgatagatg
    10681 agttaaatca ggcgctcgtt gaagccaaag gcaaaccggt gatgttagat ctttatgccg
    10741 actggtgcgt cgcctgtaaa gagtttgaga aatacacctt cagcgacccg caggtgcaaa
    10801 aagcgttagc agacacggtc ttacttcagg ccaacgtcac ggccaacgac gcacaagatg
    10861 tggcgctgtt aaagcatctt aatgtccttg gcctaccgac aattctcttt tttgacggac
    10921 aaggccagga gcatccacaa gcacgcgtca cgggctttat ggatgctgaa accttcagcg
    10981 cacatttgcg cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg gaggaaaata
    11041 gcgtgcaacg tgaagatgta ctgggagaag ccctgaaatt attagaatta caagggattg
    11101 ccaacaccac gctggagatg gttgctgaac gtgtggatta tccactggac gagctacgcc
    11161 gcttctggcc agacaaagag gcaatcctct acgatgcgct gcgttatctt agccaacaga
    11221 tagatgtctg gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa caaaagctgc
    11281 tggcacgtta tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg ggctgtctat
    11341 ttatcgctgc ctgtacgttt tatcccgatc ctggccaccc tattcatcaa ctggccgatc
    11401 agcaaaaaag cgcggcctac gatttcaccc acgaactgtt aaccacgctg gaagttgacg
    11461 atccggcgat ggtagcaaag cagatggaac tggtgctgga aggctgttta agccgaatgc
    11521 tggtgaatcg tagccaggcg gatgtcgaca ctgcacatcg gctggcggaa gatatcctgc
    11581 gcttcgcccg ctgccgccag ggtggtgcat tgacctgaca gaaacacaga aaagaagcga
    11641 tttgccgcaa tcttaagcag ttgaatcgct tttactgaaa ttaggttgac gagatgtgca
    11701 gattacggtt taatgcgccc cgttgcccgg atagctcagt cggtagagca ggggattgaa
    11761 aatccccgtg tccttggttc gattccgagt ccgggcacca aattcatatc aacggacctc
    11821 cacggaggtc cgtttttcgt ttcaggacgc cacgatttaa gcgttctgcc tccaaatcaa
    11881 ttctaccgaa ctcaaccaga ttctccccac atcaccagca atttgcgagc atatcccaat
    11941 ttgggaaaat ttgttcctga gctatagcac taactgacgt gaaatgtcgt gcggccccgt
    12001 gatgctgttg aacgtcaaat gacgtcatca ggagcgtaac gtatccataa agcacaacac
    12061 cggtctgaac gccgactgat gagattttct gaatgagaac aaagagaaat gtgtcagtcc
    12121 gcttgctcat gcaaagacta aca
//
LOCUS       DAJTNM010000116        12112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTNM010000116 DAJTNM010000000
VERSION     DAJTNM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.116"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(124..291)
                     /locus_tag="OO172_003280"
     CDS             complement(124..291)
                     /locus_tag="OO172_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513354.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHHAA"
     gene            complement(479..1318)
                     /locus_tag="OO172_003281"
     CDS             complement(479..1318)
                     /locus_tag="OO172_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5513355.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVAYIFKHPAGKLFWALTDESNKEQADVIPVYAAAPASVVPDNASEPLAYAYKELT
                     PEIMRNHLAVFERYGIAPNDSSTTIQALRIALDGIERSDAMLHGAEPVSQTYKLPPLS
                     SNEVNDAAWKLHNMLTEHGPLNGRQFNNLKGCFYEALKVAMRNYPVTPDGWISCSERM
                     PEDTKMLLAFSQGQIVAAYWNWVMSPIDYKKYRAFTYLSGNILDDVTHWMPLLEPPEQ
                     DGE"
     gene            complement(1320..2006)
                     /locus_tag="OO172_003282"
     CDS             complement(1320..2006)
                     /locus_tag="OO172_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5513356.1"
                     /translation="MSEINYQALRKAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVAQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEGKDSKIANLTAERDALREGEMGDARYSNTRAAADIYFQLVEECEIPAGGSLV
                     EYVDDMREKLESAEKRIAELEEREILLPERSSMLHRTDFHEDYQTVMAYKVSEVIDAI
                     RATGIRIKGE"
     gene            complement(1993..2307)
                     /locus_tag="OO172_003283"
     CDS             complement(1993..2307)
                     /locus_tag="OO172_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513357.1"
                     /translation="MTITKQRVEKIIYRHEMGLSSDVTAEEVYDLAVLALNLSNIANL
                     KRYELDMGGCDSCGQDCGADMTEDSDGDYVLFDDVVKLFEFDTTTQKLESPAKEATSE
                     RN"
     gene            complement(2304..2789)
                     /locus_tag="OO172_003284"
     CDS             complement(2304..2789)
                     /locus_tag="OO172_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513358.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVNETNAEYLVRKFAEAEAKCA
                     ALAAENAGLKAALNHEVIPDTAVEAFNETAIIDHDWNDTSEWSWVENDTDVIRAVLEA
                     IKPETPATDAFLAEIERKAIRKFINSIEHILRDKLSPYDTEEMLETMRIFLEEQGGEQ
                     K"
     gene            complement(2786..2950)
                     /locus_tag="OO172_003285"
     CDS             complement(2786..2950)
                     /locus_tag="OO172_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCR5513359.1"
                     /translation="MRGLAYNLGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRNGKK"
     gene            complement(2961..3254)
                     /locus_tag="OO172_003286"
     CDS             complement(2961..3254)
                     /locus_tag="OO172_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCR5513360.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3268..3774)
                     /gene="ssb"
                     /locus_tag="OO172_003287"
     CDS             complement(3268..3774)
                     /gene="ssb"
                     /locus_tag="OO172_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR5513361.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(3775..4482)
                     /locus_tag="OO172_003288"
     CDS             complement(3775..4482)
                     /locus_tag="OO172_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR5513362.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIRIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKHGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(4737..4889)
                     /gene="kil"
                     /locus_tag="OO172_003289"
     CDS             complement(4737..4889)
                     /gene="kil"
                     /locus_tag="OO172_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCR5513363.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(4874..5005)
                     /locus_tag="OO172_003290"
     CDS             complement(4874..5005)
                     /locus_tag="OO172_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HCR5513364.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVDVLLCAMK"
     gene            complement(5030..5998)
                     /locus_tag="OO172_003291"
     CDS             complement(5030..5998)
                     /locus_tag="OO172_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HCR5513365.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVHDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAKRERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(6166..6408)
                     /locus_tag="OO172_003292"
     CDS             complement(6166..6408)
                     /locus_tag="OO172_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513366.1"
                     /translation="MRVKTMGASLLSGRIFQGTLNTEKGMWVGKREDVTDQAVRAVAE
                     HMMIKDQKYAYETKDGKWLIISHQLVDKLPEEFVDG"
     gene            complement(6611..6943)
                     /locus_tag="OO172_003293"
     CDS             complement(6611..6943)
                     /locus_tag="OO172_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5513367.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRTERKNKRTWYSKPRSEIGVTCSGRQKQRG
                     KSIPAYYD"
     gene            complement(7404..7808)
                     /locus_tag="OO172_003294"
     CDS             complement(7404..7808)
                     /locus_tag="OO172_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513368.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGSMNGDRYEFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            complement(7805..8437)
                     /locus_tag="OO172_003295"
     CDS             complement(7805..8437)
                     /locus_tag="OO172_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5513369.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            8541..8756
                     /locus_tag="OO172_003296"
     CDS             8541..8756
                     /locus_tag="OO172_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5513370.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            8876..9169
                     /locus_tag="OO172_003297"
     CDS             8876..9169
                     /locus_tag="OO172_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HCR5513371.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            9202..9348
                     /locus_tag="OO172_003298"
     CDS             9202..9348
                     /locus_tag="OO172_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HCR5513372.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            9341..10174
                     /locus_tag="OO172_003299"
     CDS             9341..10174
                     /locus_tag="OO172_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HCR5513373.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTDKEYNNIISNTDVLESTAA
                     DKKSDKKKPSVSCQDVVDAYHEILPEAPKIRALNDKRKNQIRTFWRKAGVITRQLDGH
                     GFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGFDFLLNDNTYLKVREGEHDD
                     R"
     gene            10164..11600
                     /locus_tag="OO172_003300"
     CDS             10164..11600
                     /locus_tag="OO172_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5513374.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPSETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            11677..>12112
                     /locus_tag="OO172_003301"
     CDS             11677..>12112
                     /locus_tag="OO172_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR5513375.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRA"
BASE COUNT         2925 a         2973 c         2701 g         3513 t
ORIGIN      
        1 ttttcagtcc acatgcgatt gaggcaagag ttgcgctact gatgccaaca tcgccagtcg
       61 attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccaa taaaaaaccg
      121 ccatcaggcg gcatggtgtt ctttcagttc ctcaattcga atattggtta cgtctgcatg
      181 tgctatctgc gcccatatca tccagtggtt atagcagtcg ttgatgtcct ctgcttcgat
      241 aaccctgtcg aatggctctc cattccattc acctgtgact cggaagtgca tttatcatct
      301 ccataaaaca aaacccgccg tagcgagttc agataaaaga aatccccgcg agtgcgagga
      361 ttgttagttg ctatctgctg cttccttggt cattaccata tccacccaat ttcaccagac
      421 atgattctcg caatcactat tatcaccaaa gtgataatca caactttaac tggcggcatc
      481 attcaccatc ctgctccggc ggttctagca gcggcatcca gtgggttacg tcatccaaga
      541 tatttcctga taaatatgtg aaagctctat attttttgta atcaattgga ctcattaccc
      601 agttccaata tgctgccact atttgacctt gactaaatgc cagtaacatt ttggtgtctt
      661 ccggcattcg ctcactacag cttatccaac catccggagt taccggatag ttgcgcattg
      721 cgacctttaa tgcctcatag aagcaacctt tcagattgtt gaactgacgc ccatttagcg
      781 ggccatgttc agtcagcatg ttgtgtaatt tccatgccgc gtcgtttact tcgttggatg
      841 acaggggagg caacttgtaa gtttggctta caggttcggc accatgaagc atggcgtcgc
      901 tccgctctat gccatccagc gcgattcgta gtgcctgaat tgtggtagag ctatcgtttg
      961 gggctattcc atatcgctcg aatacagcta aatggttgcg cataatctca ggcgtaagct
     1021 ctttgtaagc ataagcgaga ggctctgatg cattatccgg cacaaccgac gcaggcgcgg
     1081 cagcataaac aggaataacg tccgcttgct ctttattgct ttcatccgtt aaagcccaga
     1141 ataatttccc ggccggatgt ttgaaaatat aagcaactgg ctctgcttcc agcgatgcca
     1201 gtgcaattcg tgccagttct tccgcttctt ctgctggcag tacaacgttg ctaccaggtc
     1261 cgtatgtttc gcgccactgc ttgattgtca gtagtcgctc tttggtaata gtggtcatct
     1321 cactctcctt tgatgcgaat accagtagcg cggattgcat cgatgacttc agaaactttg
     1381 tatgccatta ccgtttggta atcctcgtga aaatctgttc gatgaagcat gctgctacgt
     1441 tccgggagca gtatttcccg ctcctccagt tctgctatgc gcttctctgc ggattccagc
     1501 ttctcgcgca tatcgtcaac gtactcgacc agagaaccgc cagcaggaat ttcgcactcc
     1561 tcgaccagtt ggaagtagat atcagctgcg gcccgtgtgt tgctatacct agcgtcgccc
     1621 atctcacctt cacgaagagc atcgcgttcg gcggtaagat tggctatttt gctgtctttg
     1681 ccttccagct caacgcgcag cttccctacc gtaagcgcaa tatcctcgtt ctcctggtcg
     1741 cggcgtttga tgtattgctg gttcctttcc cgttcatcca gtagtgcctg cgctacttca
     1801 gggttgaaag ctgcgatata acgagcgttg ttctctgcgt tgttctgtcc atcaaagccg
     1861 gtccatttga taacgtcttc acatcgttta tcaccaggtg tatgcaccgc atatgtacca
     1921 gtaccagacg aaataaatgc gacccattca ccctgtgttg ccagttgcgc cgctttacgc
     1981 agtgcctggt aattaatttc gctcactggt tgcctccttt gctgggcttt ctaacttttg
     2041 agtggttgta tcaaattcaa acaacttaac cacgtcatca aacaggacat aatcaccatc
     2101 agaatcttca gtcatgtcag cgccacaatc ctgaccgcat gagtcacaac cacccatatc
     2161 aagctcgtat cgcttcaggt ttgcgatatt tgataaattc agcgccagta cagccaggtc
     2221 ataaacctct tcggcagtga catcgctgct cagtcccatt tcatggcgat atatgatttt
     2281 ttctactcgt tgttttgtga ttgtcatttt tgctcgcctc cctgttcttc cagaaaaata
     2341 cgcatagtct caagcatctc ttcggtgtca tacggtgaca acttgtcacg caggatgtgt
     2401 tcaatgctgt taatgaactt gcggattgct ttgcgttcaa tttcagccag aaaagcatcg
     2461 gttgccgggg tttcaggttt aatggcttca agcacggcgc gaatgacatc ggtatcgttc
     2521 tccacccatg accattcgct tgtatcattc cagtcgtggt ctatgatagc tgtttcattg
     2581 aatgcctcga ctgccgtatc cgggattact tcatgattta aagctgcctt cagccccgca
     2641 ttctccgccg ccagcgccgc gcacttggcc tccgcttcag caaatttacg caccagatat
     2701 tcagcgtttg tttcgttaac ctttaaatca cttgggatgc atttaccttt cagaaatcca
     2761 tccatctcaa ttagtgtcat ttgtttcatt tcttcccatt ccgccacatt gcattcagat
     2821 atttgttttg atttactgac ggaaaagaat ttcttttaag caattcctct ctcgatggca
     2881 ttggctttac gcgttggcga ataatcattt ctgccggaag aatgccgaga ttgtatgcaa
     2941 gtcctctcat ggtaaattcc tcagtcatta ctgatagcgc catagcgtga gcggtaatta
     3001 cgcaggcgcg ggtcaatttc agggaagtgg gtatatgtgg ctttgcggaa tggtcggatt
     3061 gatgtctggt aaattcgctc gcgctcttct ttctctgcaa gccatatgca gtggcgaaat
     3121 tccttttcct ctttcgtttc ctgcggtagc gacattatcc ggtcgtagtt tttcctgaat
     3181 ttatccagca cctccgatac ggaattgccg gaacagcggc gcgggtcatc cgcaccatac
     3241 tgaggcgctg gcatgatttt ctcctgatta aattgcgtga atagcgtgac gagggaaggg
     3301 gagagttact ggtgcaaagg gtatatcgtc gtcaaaatcc atcggaggtt cgttgtgttg
     3361 tgctggtgat gattgctgct gtggcttctg tgattgcctg ctggctgctt gttgtttgct
     3421 gtcgccaatg ccgccaagca tttgcatcac gccattaatt ccgacatgaa cctcggttgt
     3481 gtaacggtct tgccctgact ggtctttcca ctttctggtt ctcagcattc cctcgaaata
     3541 aatctgatca ccttttttca catactgccc cacgacctca gccagtttcc cggatacagc
     3601 aacacgatgc cattcagtca attccttttg ctcgccagta tttttatctc gccattgttc
     3661 tgacgtggct attgtcaggt tagcgaacgc tgttcctgat ggtgagtatc gaacttccgg
     3721 gtcttgtcct acccgaccaa ggataatcac cttatttacg cctctgcttg ccatttatgc
     3781 cgcctgtttt agttcgttaa ctctgatgtt cattacctga acgcatttag cctgcgcttc
     3841 ctcgttgcca gccattaatt gccagtcacg ctgataacgc tcgatgagtt ttttcttgtc
     3901 agtttctgtt gacgcataat cgctgaagtc tttcaggatt tgttcgcagt caaccgatgg
     3961 agatttctgg ttggtatttt ctggtgatgg tttgttatct gatgctggga ttgcccatcc
     4021 cggcagcgat ggagggagcc agtaaaatcc tgttccatcc ttcagttttg ccctgtgcca
     4081 cccatgcttt ttatcgagag atgtttgtgc gaaaccttcc tcaaggttat acagataccg
     4141 accgattccc cactgaacgg cagcacgctt cattgcacct gaacgaccac ctttgacggc
     4201 ttctacctgc gtgttttcag cagcatccca tttggttacc cattcggaat caatcctgat
     4261 tgatatgccg cattcaacgc ctccgttgtt gggaatatcg cggtattcat tgcgccatcc
     4321 tgctttgccg caaacatcgt ccaggcgttt catgattgcc cggttcgtga cataagccag
     4381 caccatagcc cacactttgc catcgcgtgt tttaccgctt tgctgtattc gccattcgat
     4441 atcttcaggg ctgaatggct catcgaattt attcaaatcc ataaatcacc tcagaatgga
     4501 catggcccaa gaaaataacg ctgatttaat acttcgactc gggacaaatt aaggcatacc
     4561 cgcattcctt cgcggtcgcc attatggcga taccagagag ctttctgggt gtacatgcgt
     4621 ctctgtaact tgctctcctt cactgtggtt gcaagtgaca tgaatatctc cttcgtttcc
     4681 gattaattct ttcatctgac gaatgaattc ttcgtctgac cagttatctg taaaactcat
     4741 ttcctgcgat accacggaag gttgatagct gacttcatcg ctttatttgc ttcaagccac
     4801 attttggaat caccaataaa tctggctatt actgctttgt tctgtgcagc acgaagcatc
     4861 tggtgattga tggctatttc attgcgcata ataagacatc aactcttttc catccgtcac
     4921 gtaatttacg ggtgattcgt tcaagtaaag attcatttaa ttggaaggca cccatgcgag
     4981 cgcctcccgc gattgcgtaa atcatgggtg gttccttatg ttggttttat tagtaggtta
     5041 tttttgttgc gaatacttcg ccttttacga tggctgttat gatattttta gcaacatctt
     5101 ctgatgcgcc aaccttgata aggtcagcaa gtattttgtt atttacttct tttcggtgag
     5161 ctttatcctt tgctctacgc tcttcttcgt ccttgattct tttttcttct gctattctgg
     5221 cttgctcttt tgcttcagct tcgcgccgga ttcgttcagc ctcctcctgt gcttttcggc
     5281 gttctgcttc aattgccgcc tgcttttctc tttcagctcg ttctgctgcc tcttttgctt
     5341 cgcgctgtgc tcgttgctcg gcttcaatgc gttcacgctt tgcacgttcc gctgcggcct
     5401 tagcttctgc ttctcgcctt gctgctgctt caatttcggc ttttgccttt gcttcggctt
     5461 ctgctctggc tttctcttca gcttctcttt ttaagcgttc ttcatgctct cgcttttcct
     5521 gctccgcttt gagtcttgcc tcttctcttt ggcggtcaaa ttcgcgatcc atcaaaatcg
     5581 ctatttcatg gtcagactca atttgctttg cgagagcttc agctgctgcc ttagcttctt
     5641 cttcggcttt aatccgcgcc tgttcctcct cataatcagt aagaggctgg cgcgccttgg
     5701 ctttcagctc atcaagtcga tcatgcactg ttttgcggtt ggcatcaatt agctttggaa
     5761 tttccttcag ttcagcaaca aggtctttgc caagaccatc gagatatgtt ttcgtctgcg
     5821 caactttata cgccagagaa gcgatctcct ttctgccctt tgccgttgtg atatcaggca
     5881 caaaggacat aacttcacgt tcaacctttt gaaggatttc ttcaatctgg tcggcagact
     5941 gaaatacagt cattgcattt gctttttcaa taacaactaa atctgttact tcactcatat
     6001 ttcccccgtc aaaaaaattg ccctcacact ggagggcaaa gaagatttcc aataatcaga
     6061 acaagtcggc tcctgtttag ttacgagcga cattgctccg tgtattcact cgttggaatg
     6121 aatacacagt gcagtgttta ttctgttgct tattccaaag caaatttaac catcaacaaa
     6181 ctcttctggt aatttatcaa ccagttgatg acttattatc agccatttgc catccttcgt
     6241 ttcgtatgca tatttctggt cttttatcat catgtgttcg gctactgccc tcactgcctg
     6301 atcggtgaca tcttctctct ttcctaccca cattcctttt tcagtgttta atgttccttg
     6361 aaaaatacga ccacttaata ggcttgcgcc catagttttt actctcatgt atcagtcctc
     6421 aaataagtgg tttgctgcga aagtaaatac gcttaagtta cctgttattt atcccaccaa
     6481 gttccgtatc tatctatcca gttacaccaa tcatcgacac tctattttgt tgtgtcgcat
     6541 tttggcaact ggcaagaata tctaccttct ttgtaaagtc ggcgtttgac tttcttgagc
     6601 atggctcacc tcaatcgtaa taagctggaa ttgattttcc gcgttgcttc tggcggcctg
     6661 agcaagtcac acctatttca ctgcgtggct tgctgtacca tgtgcgctta ttcttgcgct
     6721 cagtacgctg caggttgctt tcaatctgtt cgtggtattc agccagcacc gtaaggtcta
     6781 tcggattcag tgcgctttct actcgtgatt tcggtttgcg attcagcgag agaatagggc
     6841 ggttaactgg ttttgcgctt accccaacta acaggggatt tgctgctttc cattgagcct
     6901 gtttctctgc gcgacgttcg cggcggcgtg tttgtgcatc catctggatt ctcctgtcag
     6961 ttagctttgg tggtgtggtg gctggtagtc tagctccagc ttgttgagtc tcattcggag
     7021 gggtataacc ggcaccccag cgatttttcc atgcgacaac gtgcgcgtta tggcggcctt
     7081 atcgcccgcg gctccccatc tcgtccacgc tattgctagc gttgggagcg cttcaccgct
     7141 caacagtagg taagcacttg ccagtgacta gctggcttca ccacacccca aagccttctg
     7201 ctttgaatgc tgcccttctt cagggcttat tttttaagag cctcaccttc atggtggtca
     7261 gtgcgtcctg ctgatggctt aaaattacaa gaaagattgt atgttgtaaa caagaaatat
     7321 tgtgaaaagg ggcgtgaaaa acaaactcca ttgtttttaa atgaaaaata gtttgttttt
     7381 ggttatcgag attgaggtgg ggattactgg ttgcaggttc cgactacatc accaacaaat
     7441 gatttggttg atgtaagttg ttgcatacct gggatattca ttactttgga gtaaagagct
     7501 tttttgtctg tagtgattga ccaagtttca acggttattc ctcctccaga ctggtattct
     7561 cctaccatag tgttcgatga caaagcagtg tatttcatct ctggatagac gccagaaact
     7621 gattcataaa ctgatgattt atcgccattt attgttacgt ggaaaacgga atcttccgtg
     7681 ctgtcttttg taaactcgta acgatcgcca ttcattgacc cgtacccgtg caggtttgtg
     7741 acaatccagc attcagaatt ggcgctggta gttaagagta ttgagagtag cgccgcaatc
     7801 ctgatcatac gaattttacc ctcgcttcca cgacaacacc gataatcttg cagttcccgt
     7861 tgataggagt cataggccat gaagggttca ggcctttcag gtacttctgc ccgccatcta
     7921 taaccagttt cttgaatgtt gcttcgttcg cgtcagtcag tttggctaca acaaggcttc
     7981 cattcactgg ctcgcgtcca gtatctacta acaccatatg accttcaggg atgctttgac
     8041 ctacaggtga ggtcatggaa tcaccttcaa ccttcagcca gaatccatcg cctaataagt
     8101 taacgtcact gtcataccat tcatcaatgt ccttgatatc gtagggttca caagcttcac
     8161 accacgaacc agctctaacc atgctaatca atggatattt ccctttgggt tcaacatgcc
     8221 caacaaatct aacattcgaa tcagaggtgc cattgagcag ccagtcaaca cttacgccaa
     8281 gagctgacgc aagttctggt aaaaagcgtg gtcgcttagt tttaccgttt tcgagctgct
     8341 ctatagactg ctgggtagtc cccacctttt gagcaagttc agcctggtta agtccaagct
     8401 gaattctttt gctttttacc ctggaagaaa tactcataag ccacctctgt tatttacctc
     8461 caatcttcac aagaaaaact gtattttaca aacaagatac attgtatgaa aatacaagaa
     8521 agtttgttga tggaggcgat atgcaaactc tttctgaacg cctcaagaag aggcgaattg
     8581 cgttaaaaat gacgcaaacc gaactggcaa ccaaagccgg tgttaaacag caatcaattc
     8641 aactgattga agctggagta accaagcgac cgcgcttctt gtttgagatt gctatggcgc
     8701 ttaactgtga tccggtttgg ttacagtacg gaactaaacg cggaaaagcc gcttaagaca
     8761 ttcccgctct tacacatccc agtcctgaaa aagggcatca aattaaacca cacctatggt
     8821 gtatgcattt atttgcatac cttcaatcaa ttgttatcta aggaaatact tacatatggt
     8881 tcgtgcaaac aaacgcaacg aggctctacg aatcgagagt gcgttgctta acaaaatcgc
     8941 aatgcttgga actgagaaga cagcggaagc tgtgggagtt gataagtcgc agatcagcag
     9001 gtggaagagg gactggattc caaagttctc aatgctgctt gctgttcttg aatggggtgt
     9061 cgttgacgac gacatggctc gattggcacg acaagttgct tcgattctca ccaataaaaa
     9121 acgcccggct gcaaccgagc gttctgaaca aatccagatg gaattctgag gtcattactg
     9181 gatcaatcca caggagtaat tatgacaaaa caactcagtc cttaccagga caaaattcac
     9241 aaacacatac tacgtgatcg cttcctgtcc agcttcaagc agcctggtcg attccgggct
     9301 gagttggaaa aagtgaagct gatgcagaag gagaaaggtc atgagtaaca tatctaatct
     9361 agccgaagcc agagaggcca gaaggctaca acaaccgcat caaagcagcg gtaaggggta
     9421 tgccttgctg caccgtaaaa ttatggatgt gccgttttac aaggacgcag aagcagcgca
     9481 tctgtgggtt cacttaatcc tcaaagcaaa gcatacgcct gagtatgtaa tgactgacgc
     9541 aggagaaatt ctggtaggca gagggaagct acttggcggt agaaactctc tggcgtttga
     9601 aacaggactc aaaccagatc gcgttcagta cctgcttaga aagttcaaaa aactcggcat
     9661 gattgactgg gtttcacacg gtaaattctc agttttctcg gtagagaaat atgacgatta
     9721 tcagtcaaat tttgtaccag cagattacca gcaaattacc acagataaag aatataataa
     9781 tattatctct aatactgacg tattagagag taccgcagca gacaaaaagt ctgacaagaa
     9841 aaaaccttcc gttagctgtc aggatgttgt cgatgcttac cacgaaatcc ttcctgaagc
     9901 gccaaaaatc cgcgcactga atgacaagcg taaaaaccag atccgaacgt tctggcgcaa
     9961 agccggagtg ataacccgcc agcttgacgg gcatgggttc acgatgcagg actggagaaa
    10021 ttatttgagc tacgtaggcg aaaattgccg atggatgttc gaagagcgcc caaaccatca
    10081 gcgcggaacc gtctggcaca aaaagggatt tgatttcctg cttaacgata atacctacct
    10141 gaaagttcgt gagggtgaac acgatgaccg ataattttta tgcgccgccc catagcatcg
    10201 aggcagagca ggcggtgatt ggtggattgc ttctggatga tgacagcagt gagcgcgtcc
    10261 agaaggttct ggcgatgctg aagcctgatt cattttacag ccgaccacac aaaatcattt
    10321 tcgaagaaat aaccagaatg caccgggagc aaaagccagt agatggcctg acgcttttcg
    10381 atgaactgga gcgtaaatcg ttaacggcgt ctgttggcgg ttttgcttat atcgctgaga
    10441 tcgcaaagaa cacgccaagc gccgcaaaca tcgttgccta tgcaatgcag gttcgcgaaa
    10501 ccgcaatgga acgctacgcc atcaaccgca tgactgaagc gacggaattg ctctattccc
    10561 gcaacggaat gactgcgacg cagaagtacg aagctattca ggcgattttc acgcaactga
    10621 cagaccatgc aaaaacagga tcgcgtcgcg gccttcgctc atttggtgag gtcatggaag
    10681 actgggttag cgaccttgag aagcgatttg acccatcagg cgaacaacgg ggaatgagca
    10741 cagggatccc atcgctggac aggatgctgt caccgaaagg tctggtgaaa ggctctctgt
    10801 ttgtcattgg cgctcgacct aagatgggga aaacgacgct atacagccag atggcaatca
    10861 actgcgcagt gcatgagaaa aagcccgctc ttatgttcag ccttgaaatg ccaggtgacc
    10921 agatactgga aaaactggta gggcagaagt ctggtgttaa cccgaatatt ttttaccttc
    10981 cggcgacaaa tgacgctgat gacggctatc agggtgatta cgatggtgac ttcaacaggg
    11041 cgatcgaaac agccaatcgc ttgagtgaaa tcgacctgct ttacatcgac gacacgccgg
    11101 gattatctct ggctcaaatc gtcagcgaaa gccgtcgaat caagcgagaa aaaggatgtg
    11161 ttggcatgat tctggtcgat tacctgacac taatgaccgc tgaaaaggcc gatcgcaacg
    11221 accttgctta cggcatgatc accaaaggac tgaagaacct tgccaaagag cttgattgcg
    11281 ttgttgtgct tctgacacag cttaaccgcg cactggaaag ccgaaccaat aaacgcccat
    11341 taccaagtga ctcacgagat acagggcaga ttgaacagga ttgcgattat tgggtgggga
    11401 tccatcgtga aggtgctttt gatgacagtg ttccacctag tgaaaccgaa ctaatccttc
    11461 gtctcaatcg tcatggcaat accggcacgg tgtattgcat tcaggcaaat ggcgctattt
    11521 atgacacaga ccaacagtct gctgaaatgc gccgacgtga acgcgaggaa ccgcagtcca
    11581 agaagaaagg aggattctga tgaccatcta catcactgag ctaataacag gtctgctggt
    11641 aatcgcaggc ctttttattt gggggagagg gaagtcatga aaaagctaac ctttgaaatt
    11701 cgatctccag cacatcagca aaacgctatt cacgcagtac agcaaatcct tccagaccca
    11761 accaaaccaa tcgtagtaac cattcaggaa cgcaaccgca gcttagacca gaatcgaaag
    11821 ctttgggctt gccttggtga cgtctctcgt caggttgaat ggcatggtcg ctggctggat
    11881 gcagaaagct ggaagtgtgt gtttaccgca gccttaaagc agcaggacgt tgttcctaac
    11941 cttgccggga atggctttgt ggtaataggc cagtcaacca gcaggatgcg agtaagcgaa
    12001 tttgcggagc tattagagct tatacaggca ttcggtacag aacgtggcgt taagtggtca
    12061 gacgaagcgc gactggctct ggagtggaaa gcaagatggg gagacagggc gg
//
LOCUS       DAJTNM010000117        11928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTNM010000117 DAJTNM010000000
VERSION     DAJTNM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.117"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(1..2249)
                     /locus_tag="OO172_003302"
                     /pseudo
     CDS             complement(1..2249)
                     /locus_tag="OO172_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(2273..2896)
                     /locus_tag="OO172_003303"
     CDS             complement(2273..2896)
                     /locus_tag="OO172_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5513376.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVINH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2893..4581)
                     /locus_tag="OO172_003304"
     CDS             complement(2893..4581)
                     /locus_tag="OO172_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCR5513377.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAVGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(4730..7357)
                     /gene="gyrA"
                     /locus_tag="OO172_003305"
     CDS             complement(4730..7357)
                     /gene="gyrA"
                     /locus_tag="OO172_003305"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5513378.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7504..8226
                     /locus_tag="OO172_003306"
     CDS             7504..8226
                     /locus_tag="OO172_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5513379.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8354..11928)
                     /gene="yfaL"
                     /locus_tag="OO172_003307"
                     /pseudo
     CDS             complement(8354..11928)
                     /gene="yfaL"
                     /locus_tag="OO172_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2767 a         3309 c         2939 g         2913 t
ORIGIN      
        1 tcgacattga ccagttcacc aggctggtaa tgggttttgt ccgttttcac ccggatatca
       61 agctgaggaa cggcaacttt gatcccggcg ttctgaaaac tgtactgacc attacgggta
      121 tacagcaccg aaaaagtgat gttaggcgca aaggaattgc tcactggaac ccgggcttca
      181 tacagggtat cgtttaaacg ttgtagcgtt agccagtttg ccggatgcga aagcagcgac
      241 tgctgttcca cgcgatcgcg ttccagcgtc aataacgctt catcaattgg ctccgggaaa
      301 gtaatcagca tcttcgcggt ttcgcctggc tggtacagcg ttttatccgc cacgatatct
      361 accgtaccag tatgcgccgt gctgccctta ccgctgacgg catgacttaa cccagcgaga
      421 attaagccgt ctttatcgcg taatgtcaga ttgtagttgc caggtttagc gaaattgacg
      481 gtaaaggatt tgccgcctga cggtagctct ccgctatggc tcgtgcggtc ttcgagacgc
      541 aaccattcat acgtaacagg aacctgtttt gaagattcca gcgcggcata acggaacaca
      601 accgactcgc cgctattact gtattgtgcg gcggtactta aagagtaatg cgccaggccg
      661 cgttcaatga ggagctcttt ggtggtggtg acgcgatacg ccgcgccgtc actggcggag
      721 acggttaaca aatagcggct cggtttatcg gcggcgggga gatttaacgc cacataaccg
      781 ctggcgtcgg acaccgtttc gctgccttcc agcgacacgg ggaaacgtcc ggcataacgc
      841 aaatcgttac cgaccattga taattgctga gcgcgcaaac ttaactgcac gcgggcattt
      901 tttaccggct cgccatccgg gtagagcagt tgcagtttgc cgctgaccgc ttcgccagtt
      961 ttgaactctt ttttgtcgag agctaaacca atctcgaaat gtggcttgat gtagtttgcc
     1021 acgcgaaaac tgctgctata gacctgattg cggtaagcaa gacgtaactc ataacctccg
     1081 gcaacggcat tttctggcag gcggaaactt ccctgcccgc cattgcgcgc atccagcgtg
     1141 acatcgacgg tttgcaacag actgccgtta gcgtccagca ccgaaagctt cgccggggcg
     1201 ctgacgatgg gggatgaatg caacggatcg cggaactcgc ggcccatcac tttaacatcg
     1261 acacgatcgc ctgcgcgata tagcgggcga tcggtaaaaa tatacaagcg ggtgttgtag
     1321 atttcgcttt cgtagaagaa gttctcggag acaaaaacgc cgccttcagc atccttaccc
     1381 agaatgtatg aacgttctgg cgatatatgt tgtaactgca aagtaccgct gtcatcggtc
     1441 accccgcggg tcatcacgcc aagaccgtca gtccacaaga tctctgagcc gggcttcgct
     1501 tcaccctgtt ttttacccgc ggtccatacc agaagctcgt tacctgacac tttgctaagc
     1561 gccacggtat cggaaacaaa caccaccgtc gtcgcccgat acccacctac catcgcctcg
     1621 acgaggtaca gccccggctc ttgttggccg agaggaatat aaatattacc cggctgcggc
     1681 gagatgaaat tgctggatgc gccttccagt tttatccctt gctgcggctc gaccggttta
     1741 gcctgccata gtggataacg gaactgttcc accaagggat attttttcag cggggaaaac
     1801 tggttgttct gtacataacg ggaaggttta ataatggcat tgccgagatg taattcgggt
     1861 aatgcctgag tcacattctg ccgtgactga gaagagaaag tacgctgcat cacgcggcga
     1921 gatttgccgt accagttatc ccacagctag gtcagcgtat tgttcagccc gtcgcccaga
     1981 tattgcggtt gcaccacaat gcgatgcagg tttttctgct ggcgcaaaaa tgccatcggg
     2041 tcaggaatac gatacaggcg aacgtccacg ccgccgtact cttccatctg ataacgccga
     2101 taatcacgcc ccggcgcttc cagtcgcact ttcgcctctt cactgctgct aaaactgctg
     2161 tcagcaagca aaaagaatgt tcccccggcg ggcggcgcat agttactgga aggaagcgaa
     2221 tcatcagctt tagcaagccc tgttcccaca agagacaggg acaagcacgc tgctatcgcg
     2281 ccagaaaatt taaacgataa atgccaatga aattgggatt ggaatcgttg ggtatccatc
     2341 gggtgtcctt ccatgtcata agttgttgca gactgactgc gcgcattccg ttgtcagttt
     2401 tcgtggcgct tccggtgtgg ttgatgacgt aacgccccat ccagaccatt aagtgctggg
     2461 catcgccctg atcgaaaaaa atcatatcgc caggcagcgc ctggtttatg tcttggccaa
     2521 taaactggct gttgtactga atcaaattaa tcgcggtcac gtaggggccg tttttcccgc
     2581 tcccctgatt ccagttttgc gccagttgac gctgttcagg tgttagcgtc atctctggcg
     2641 gcaaatactg gctagataaa ccgttacttt ttagccattt actgtcgtga actttcagcg
     2701 tctcgttcgc cgcaaatcgc accaggcccg cacaatcctg ctgataccag cgtggacttg
     2761 gcccctggcg gagctgttct tgtgcaatgc gtacaaacca ggcgcgaaac agcctggatt
     2821 gttcgacatt cagcatttcg ctatgggcaa caacacaaca cagccagcaa atcagcgcca
     2881 gcagcccgtg tctcatagcg gctgccaggt tatgggtagc cactgccagg cgacaccggg
     2941 ttccatctgg gccagcttca tgacataacg cggttgttga gataaagcgt ccagcttcgg
     3001 cattaataaa gtttgtgcgg cgttataaaa aaccggttcg agattcttcg gcagactggt
     3061 caacgtttcg ttacgcagca gtttcgccac gccttgtgga ttgatataga gcggaacgat
     3121 gccatcagtg ggtattacat ccaccattgc cgggcgggtt ttattcagtg tttgcagggc
     3181 gttattaacc agcgtgtcat cgagggagaa aagcagcgtt ttgttttgca tcgccagcga
     3241 cacacggaaa aaataatccg acattaattg atcgggttgc gccgcctgcg ctttcggata
     3301 ctgaccgtat cgggaactca cttcgcgaca ccagatttgc gcttcgccct gctgagtctg
     3361 gcttaccggc aaaacacctt ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa
     3421 cagtttccct ggcaattgcg cctgttcggc agtgccatca aactgaccga caaacagcgg
     3481 ggtttgcaat tttgagtctt cataccagca cagccccacg gcaccgtcta acgccccatt
     3541 cagcttgtcg ttttcctggc tgatgtgcga aagcatctct tcggcaatac cgtgtgaata
     3601 cggcaccgcc acacagaagc tggctccggc aggcatactg ttccagaccg gcgtaaaatc
     3661 gaaactggca tctacgctgg cggattcatc atttaacgcc acaaaactgt gccagccgtc
     3721 gttacccatt tcgaagcgta cgccagcaaa agaaggcatt aaccgctggt agccaaaacc
     3781 cagcaacctg gcgctggcca cgatgcgcag gcgtactggc gttttttcgg ctgtacgctc
     3841 ttccaggcca aagctggctt gccagcgttt tttaccgctc aacaaatcac ttgcgatcgc
     3901 cgtggcttcg gtatcctgct gatcatcttt aaacaacata tccgtactgg aaaacaccag
     3961 catcttgtcc tgatatgtcg cgaacatcag ggcgttattg ccgttatagc gcaactgata
     4021 aacaggaata gtttcactat ttatcttgat gctactgatt tccgttttgc ttaactggct
     4081 gtcgctggta gcggcaaaca acaatggctc cagcagtttg cttaaaccac tgcgctggat
     4141 cagcaccatg taatgtgaaa gatggccttg tttatcgtgc catagcgccg cctgcgcggg
     4201 ctgatctaag agtgacgaaa agagcttatc tttcagcgtg agatcgtgtt catagacaat
     4261 gcggcgaatg ctgccttcaa tgcccagacg atctgcatga ttctgataat agaaaacgaa
     4321 atcctcgctc agaacatcgt ggagaaacgg aatggtgagg agatctttgg gaagctggct
     4381 cagagagtcg ctgtctagaa agaggtccgg ctcattgaga tcgatttgca gattgttgtg
     4441 caccaccagc ggcgacaacg ttttttctgg cccactgcca gcatattgca acgcccagac
     4501 gccagcggaa agcagtgcta ttgcgccaaa acctacaaga ccatagaacc gccagccttt
     4561 cgcctttttt tcaccactca ttgccacatt ccttgtgtat agccagccat tttttacggg
     4621 cacagccaaa ctttaccgcg ccctaatacg acaaaagccc agactttgca gcctggactt
     4681 ttcaattcaa acaagggaga tagctccctt ttggcatgaa gaagtaaaat tattcttctt
     4741 ctggctcgtc gtcaacgtcc acttccggag caatttcatc atccccttcc gcggcactgc
     4801 cgtcgatggt atccagatct tcctcgtcaa ccggttcagc aacacgctgc agacccacta
     4861 cgttttcatc ttccgcagta cggatgagga tcacgccctg ggtgttacgg cccacgatgc
     4921 tgatttctga aacgcgagta cgtaccagcg taccggcatc ggtgatcatc atgatctggt
     4981 cgcagtcatc tacctgcacc gcgccaacca ctaaaccgtt acgttcggta accttgatgg
     5041 agataacccc tttcgtcgca cgcgacttgg ttgggtattc cgccactgca gtacgtttac
     5101 cgtaaccgtt ttgcgttgcg gtgaggattg cgccatcgcc acgaggcacg atcagagaga
     5161 cgactttatc gccttcacct aagcgaatac cgcgcacacc agtggtattg cagcccatcg
     5221 cacggacaga agactcttta aagcgcacca ctttaccttc ggcggagaac agcattactt
     5281 catcttcgcc actggtcaga tcaacgccga tcagctcatc gccgtcaacc agtttgatcg
     5341 ccactttacc ggcggtacgc agacggttga attcggtgag gacggttttc ttcacagtac
     5401 cgttagcggt cgccatgaag actttcacgc cttcttcaaa ctcggtcacc ggcaggatcg
     5461 cggtgatacg ttcgtcctgt tccagcggta gcaggttgac gatcggacga ccgcgcgcgc
     5521 cacgagtggc ttccggcaac tggtaaactt tcatcgaata gacgcgacca cggctggaga
     5581 agcacagaat atggtcgtgc gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt
     5641 taatacgtgc ggcagattta cctttcccgc cacgacgctg cgcttcgtat tcagaaagcg
     5701 gctgatattt aacgtagccc tggtgagaga gcgtcacgac cacatcttcc tgggtgatca
     5761 gatcttccag gttgatgtct gcgctgttgg cggtgatttc agtacgacgt ttgtcaccga
     5821 actgttcacg aaccagctcc agctcttcgc ggatcacttc catcagacga tcggcgctac
     5881 caagaatacg caacagttcc gcgatctgat ccagcagctc tttgtattcg tcgagcagtt
     5941 tttcgtgctc aaggccggtc agtttctgca aacgcagatc cagaatcgcc tgagcttgct
     6001 gttcggtcag gtagtacaga ccatcacgca cgccgaactc tggctccagc cattccggac
     6061 gcgcagcatc gtcgccagca cgttccaaca tggcggcaac gttgcccagc tgccacggat
     6121 tagcaaccag cgcagttttc gcttctgcag gcgtcggcgc atgacggatc agttcgatga
     6181 tcgggtcaat gttcgccagc gccacggcta atgcttcaag gatatgagca cgatcgcgag
     6241 ctttacgcag ttcgaaaata gtacgacggg tcaccacttc acggcggtga cgaacaaacg
     6301 ccgcgatgat gtctttcagg ttcatgatct tcggctgacc atggtgcaat gccaccatgt
     6361 tgataccgaa agaaacctgc aactgggtct gggagtagag gttgttgagc acaacttcac
     6421 cgaccgcatc gcgtttcact tcaatcacga tgcgcatacc gtctttgtca gactcgtcac
     6481 gcagcgcgct gatgccttcc acgcgttttt cttttaccag ttccgcaatc ttctcgatca
     6541 ggcgcgcttt gtttacctga tacggaattt cgtggacgat aatggtttca cgtccggttt
     6601 tggcgtcaac ttccacttca gcacgggcgc ggatatatac cttgccgcga ccggtacggt
     6661 aagcttcttc aataccgcga cgaccgttaa tgattgccgc cgtcgggaag tccggccccg
     6721 ggatgtgttc catcagccct tcaatgctga tgtcttcatc atcgatatac gccagacaac
     6781 cgttgatgac ttccgtcagg ttgtgcggcg ggatgttggt tgccatacct acggcgatac
     6841 cggaagaacc gttcaccagc aggttaggaa ttttggttgg catgacgtcg ggaatttttt
     6901 ccgtgccgtc atagttatca acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt
     6961 catgggcaat tttcgccaga cggatttccg tataacgcat tgccgccgca gagtcgccgt
     7021 cgatggaacc gaagttaccc tgaccgtcta ccagcatgta acgcagcgag aatggctgcg
     7081 ccatacggac gatcgtgcca taaaccgcca agtcaccatg gggatggtat ttaccgatta
     7141 cgtcaccaac gacacgggca gattttttat aggctttgtt ccagtcattg cctagtacgt
     7201 tcatggcgta aagtacgcga cggtgtaccg gcttcaggcc atctcggaca tctggcagcg
     7261 cacggccaac aatgaccgac atcgcataat ccagatagga gctctttagc tcttcctcaa
     7321 tgttgaccgg tgtaatttct ctcgcaaggt cgctcatcta accgctatcc ctctactgta
     7381 tcccggattc aaaggtcgca aattataaca cagccgcgca gtttgaggta aacctatacg
     7441 ctttattcac atccaatgcc tgatatactc gtttgtcttg ccaattacgg agtagaagtg
     7501 ccaatgaatg ccgaaaaatc gccggaaaac cataacgtag accatgaaga gatcgctaaa
     7561 tttgaagccg tcgcctcccg ctggtgggat cttgaaggtg agttcaaacc gttgcaccgc
     7621 attaacccgc tgcgtctggg ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag
     7681 gtgctcgatg tcggttgtgg cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg
     7741 acggtgaccg gtctggatat gggctttgag ccattgcagg tggcaaagct gcacgcgctg
     7801 gaaagcggca ttcaggtgga ttacgtgcag gaaaccgtgg aagagcacgc ggcaaaacat
     7861 gccgggcagt atgatgtggt gacctgcatg gagatgctgg agcacgtacc cgatccgcag
     7921 tcagtggtca gagcctgtgc gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg
     7981 cttaaccgca acggcaagtc atggctgatg gcggtggttg gcgcggaata tattttgcgc
     8041 atggtgccca aaggtacgca tgatgtgaag aagtttatta aaccggcaga gctgcttggc
     8101 tgggtggatc agaccagttt aaaagagcga catataaccg ggctacatta caacccgatc
     8161 actaattcct ttaagctcgg ccccggcgtg gatgtgaact atatgctgca cacgcagaat
     8221 aagtgaggtt gatgtttggc cgcgccaatg cctgatgcga cgcttgcagc gtcttatcag
     8281 gcctacaaat gctctccgta ggccagataa ggcgtttacg ccgcatccgg caattgtgat
     8341 ggtcagacag cgattaccat ttcaccgtca tcgacaaaaa ccctgccgtc tgggcaaaat
     8401 catcactccc tttctgccac gccacgctgc cgcgcagaga cactcgctga ctgatattgc
     8461 ccgtgactcc cacttttatt tcaccccgta gcttcaccgc atcgtcactg atagtgctgc
     8521 catcttcttc aatttccgtc gcatggggat cgtgataata attcagatct aatgttggtg
     8581 tgacaccaac cgcggtacgc cattcgctgt gtaaacccag ccgcgtctga atatcatccc
     8641 cctgcgattg tgacacgcgc gcatggttag cggcggtaaa atcatcctgc tgcacgccct
     8701 gataaatcac ctgcgcctgc ggttcaatca ccacaccacg ccccggtaac cactgatacc
     8761 cggcttccag cgaggcgata atccccgacg aatggtaacg atccacgcca tcttcatgtt
     8821 cggaaacatc attgttaaac cacgcgtatt gcagccagct atccagccag gccccctgct
     8881 tctgattacc gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt
     8941 tatcggcgcg agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac
     9001 caaccgcccc aagcatccac tcgccatccg tgccccagca cccgctaaac aagccgccgc
     9061 taagcagcac cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat
     9121 aacgtccgcc gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc
     9181 gctccatcat aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg
     9241 cattcagcac cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg
     9301 gcgtgggatc aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt
     9361 gcgatcgcag ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt
     9421 tgacgtagcc actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag
     9481 ctgcgaaatc aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt
     9541 taaccaccac agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg
     9601 agtcatcgcc gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt
     9661 ttaccgtcag cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg
     9721 tcagatcacc attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg
     9781 tattaagctc gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt
     9841 taaatagtga ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac
     9901 catcgctgat gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg
     9961 cgccagtaac gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat
    10021 ccgcaacatc gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc
    10081 tggcggtcag ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat
    10141 gctgcccgct gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg
    10201 ccacttcacc gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag
    10261 tatcgacatt ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct
    10321 ggcggttata gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg
    10381 cgatttgtgt tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg
    10441 catcgaccac cacgtagacg ccatctttcc cgtcaccgcc gatatgcaaa ttgtcgccac
    10501 ctgcaagatc taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa
    10561 ggccatcatt gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac
    10621 cgccgtttaa cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc
    10681 cttccagcga aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact
    10741 gcgcccctgc cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga
    10801 tgatgccagc aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag
    10861 tgccattctg aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta
    10921 gctcagcctg attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg
    10981 gatcgtcctg gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa
    11041 cttcaccgtt ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca
    11101 gatcgccgga acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt
    11161 cattctctgt attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac
    11221 ttaagccgag atacataaag ccacccgccg cagtggaagg accatctcca tagcctgctg
    11281 cgctattgtt ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa
    11341 tatcaataag atagggagcc ctcacgctat tggtatatat cgccccgcca taaccttcgg
    11401 ctgtgttatt tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt
    11461 cgctattatt atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg
    11521 tatatgcctg gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat
    11581 aaattgcgcc acctttgcca tcattagcga tattattgcg aaacatggcg ttagtgacac
    11641 gtaaatcgac ggcaccagta tcattagtac cagaagaata gattgcgcca ctatagccgc
    11701 ctgcgacgtt accggaaaaa ataacatcag taagattcag cgttgagttt tctttagcaa
    11761 atatggcccc gccgttatta tattctcctg taacggtgtt attggcaaac agagtcattc
    11821 cagtttcatt ttctggtaat agtgaaaatt ccgctccttg ttgcaaaaat acggccccac
    11881 cgctggcatt attggtcata tccgaaaaaa ccagccattg cccatcgg
//
LOCUS       DAJTNM010000118        11595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTNM010000118 DAJTNM010000000
VERSION     DAJTNM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.118"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(57..396)
                     /locus_tag="OO172_003308"
                     /pseudo
     CDS             complement(57..396)
                     /locus_tag="OO172_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
     gene            complement(712..1356)
                     /locus_tag="OO172_003309"
     CDS             complement(712..1356)
                     /locus_tag="OO172_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513380.1"
                     /translation="MMKQGMANSISLTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1377..1646)
                     /locus_tag="OO172_003310"
     CDS             complement(1377..1646)
                     /locus_tag="OO172_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5513381.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPEKLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1725..2363)
                     /locus_tag="OO172_003311"
     CDS             complement(1725..2363)
                     /locus_tag="OO172_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5513382.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2348..3580)
                     /locus_tag="OO172_003312"
     CDS             complement(2348..3580)
                     /locus_tag="OO172_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5513383.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHILEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            complement(4081..6762)
                     /locus_tag="OO172_003313"
     CDS             complement(4081..6762)
                     /locus_tag="OO172_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5513384.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVKRAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSLQLS
                     YYEKNIREKQISLNEYEEVFKKSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFSAET
                     YRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(7003..8049)
                     /locus_tag="OO172_003314"
     CDS             complement(7003..8049)
                     /locus_tag="OO172_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HCR5513385.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSAGTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(8153..8302)
                     /locus_tag="OO172_003315"
     CDS             complement(8153..8302)
                     /locus_tag="OO172_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513386.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            8724..8901
                     /locus_tag="OO172_003316"
                     /pseudo
     CDS             8724..8901
                     /locus_tag="OO172_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            complement(8954..9187)
                     /locus_tag="OO172_003317"
     CDS             complement(8954..9187)
                     /locus_tag="OO172_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513387.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(9283..9906)
                     /locus_tag="OO172_003318"
     CDS             complement(9283..9906)
                     /locus_tag="OO172_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5513388.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(9995..10504)
                     /locus_tag="OO172_003319"
     CDS             complement(9995..10504)
                     /locus_tag="OO172_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513389.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            10962..11420
                     /locus_tag="OO172_003320"
     CDS             10962..11420
                     /locus_tag="OO172_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513390.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT         3243 a         2888 c         2323 g         3141 t
ORIGIN      
        1 atctggcagc ctgagttcac agataaaaca ctctccagga aacccggggc ggttcaaacc
       61 tgattctgga aaaggacttt gtggattgtt gctattgcca gtgagattga atgaagtcgt
      121 tttaactcgc tttgaatacg tcttgccccc agatttcgct gagaacgtaa ctccagaatc
      181 agagcgactt cgcaagtgcc ggttttcgtt gatggtgaac gcttggggcg acggctgtgg
      241 ctttccagac cagcgatgcc ctgagccaga tatctgcgcc accattttac gcaatgtggg
      301 ccgagaaatg ccacagcgac ggcatacaaa tcccgcatca ccggacgttt catagagttt
      361 tacccactgt agccgctgct gaatttccct gttcataatt tggcattagt tcagctcaat
      421 cccgacttaa aaagtcccta caggatgttc cggacctgag gcacagttca tatccttatc
      481 cataaaaaat ttagctatgt ctatagcatt aatcttgaca agacgacgtt agggcctggc
      541 gatgaatcat ttcccctgcc agatatcctt tggggtgcac cagaactacc gttttatatc
      601 tatataactg aaacgtccct cgccatcctt catattttga tgtagtaaca ccattcactt
      661 tcatctaccc cccgttttat taacatatag taaaaatcac ctcgccgcat tttattcttt
      721 tgaagtaaaa tcatttacca gttcacttaa agctctcttt tgttcatcat catacagaga
      781 ttgaatccgg aatttaatct cttctgcaac cagtcttgca tcgggctcat aaaatatttt
      841 tgttattcgc tgtttctgat gttcaggctg gtcttccata tttcgtaaaa ccagatgaaa
      901 taatatatca taaagtgcgc aatgccatga ataaccatca ttggttaaaa taagctccca
      961 aacccgctcc tgctcggcaa taagctgcag aacaaaggac tcaacctgtt ctgtttgttc
     1021 gggggcaaca cggtatagcg gagcaatcac agcaaaagat agttcattta accaggattc
     1081 cgcttccttc tcgctcctga acggtggaca gttttgtatt cttcgttcgt attcacgggt
     1141 aatctgctcc agaatatcct ccggcggtgt cagccactta ttcctgagca atacactggt
     1201 tgatggatga cagctggcta tttcagcaat aatttcacgt aatgtttccg ggctgaactt
     1261 cttcagctga ttgtagtgct cccgggtaaa accatgttca tcgcataaaa gttgccatgt
     1321 cagtgatata ctgtttgcca ttccttgctt catcattaat acctgttacc tgaatatcaa
     1381 cagaccggtg taccttttct gaatacctcc ccggacggag tggcaatccc cattcgcctc
     1441 agcgtggcat ctgcagcttt cgccacctcg ctgatatcca gcttttcagg tagcggcggg
     1501 gacgatatct gcgccagaca ataattccgg cgcaacaaca accactcccg ttgtgcttct
     1561 gtacattcaa catttcccat aacaaaaagc cagcggtcag aagctctgcg ccagcaacca
     1621 gcagcctcca gtttctgcgc aaccatgtct tccacaactt ccggggtact ctttttcctg
     1681 ctcaaaacat taactcactg aaataatata catatctgac ggtctcagcg aaccgtccat
     1741 gccgggctga aatcctcttc ctgaaacagc tccgtcagcc cgctaatctg tttcaatctg
     1801 agaacttcat cctggtccat acccagctcc gtaccaatgc gttcattcgt ccagcccagg
     1861 cgcgacaggt ccctgacaat atcagacatt gaggttatct gatgtttgcc cctggcccgg
     1921 ttatgtcgga tggtcgcagc aatacgtgag gcctgtccct ttctctccgg attaatacag
     1981 accaccggca gccagccctt cagacgtttt cccgtacctg cttccctgcc cagcacctgg
     2041 cgatgaaaac catccaccac caggtaatgg ttcttttcct cagacaccac caccggttgt
     2101 gtgaaaccat ctttctccag tgactgcttc agcagtttct tctcacccgg cgccatgaca
     2161 ttaggattgt aatcattggc caccacctca tctgctttta cccacagcac acagtccact
     2221 ggctcatgtg caaaaggact gacttcatga agcactctgc gcaggtgatt aagggcctgt
     2281 atggtctctt cttctggtcg ttcctggctg aagtactgac gaatcatgtt gccaattatc
     2341 tccggtgtca cagaatcccc cattccttcc tcctttcttt catacgctgc agataacgtt
     2401 catagtgccg gggtttgttc ggactgaagg agagggtccg gcaccagaaa tcattcttta
     2461 taagtgtctt acagatacgc cgccaggacg gaatatcccg gctccccagg tcattctcct
     2521 gttcatccgg gatgtcatcc gggaagcccc gcgtctgata ccagcgcagg tagacagcaa
     2581 ttttattacg gtaatgttct gccgttcttt ccggcatcac atccagcagg aacatcgcat
     2641 agctgcgcca ggtatgatgg gctggttttg atatacgttt acgcagggca aaataggcac
     2701 cgctttcatt ggcataaagc gccccgctgg cagcgcctga cacccgctca cacatcctgg
     2761 cccaggtttc cggctccaga atatggtaaa gccacagccc cttacgttgt tcaggaccaa
     2821 aaggttcaca gacacgcatg ttgcgtaacg gcacgtcggc acggtacatc aggtcataca
     2881 gaggattgta aatggcacaa gctctggcgt tatatatcca gatatcgcgg gctttccagt
     2941 catacaacgg atacatggtg taataaaacc cttcaggcga tgccgtggtc cagggcttat
     3001 catcggcata tctcagtttg cgctgagaca ccagccccat aaagcgattg agggattcat
     3061 ccgcacgaac accggtcagc actgccactc cacaccggtt accggcaaac caggaagaaa
     3121 atgccggaac aaattcttca aacgtcatgg cataccggaa aaagggaaaa tatgtcatat
     3181 ccgtaatggc ctcttccggt ggctggcgaa cccaggtcac tcctggctcc cagcatatcc
     3241 actccggctg aaactgcgag acgccgttta ccgtggtcag gggaagtgcc acccagtaaa
     3301 aggtttccgt cacatcatgg tacatttccc gcatcttctg aatatgttca atggtgcacc
     3361 gatactgggc ctcccagtca atgaacagaa cagagaaatg acgttttctc ctgcgggcca
     3421 cctctgccac aagatggaaa agcacggtgg aatcctttcc acctgaaaaa gacagacata
     3481 ccgacggaaa tgtttcaaaa atccactcaa tgcgggcgat tgcggcacgc aaaacatttt
     3541 ctccggtaag gattttttcg gttaaaagcg tatcactcat tttcctggct ttatgtcgaa
     3601 taattctgtt tatacattca ggcaaacttc attaataact caccgctttg gggataaata
     3661 ataacaacaa aaatcttcag atattaacct ggttgttgga tttgttatca catcctttat
     3721 ccacctgctt cttactggta acacatctgg aaaatccatg cattttcaac atatactatc
     3781 ctttcaactt gtaaataaga aaatatgatg cagatggtga acaattcaaa tatcattttg
     3841 caggaagtat tcactgtaat agaattaccc cctgataaag tttattcact aaggaattat
     3901 tcaacagaaa cattccctgt tagctttgag caatagcact tatttttata tgtatcagga
     3961 atacgtacgt tgatgtaacc aaaacaaaga agcctgtgtc catggggttc gtacaattgt
     4021 gtgtggcaca tgaccaaatc aggtttgaga cctcccgctg caatattttt tattcagatt
     4081 ttagcctgtc aggcctgaca gaaacgctcc caacttccct tctatatcac tgtctatagt
     4141 acgtaatctt agcaatggca ggccgcattt cttcagtata ctgtttttaa gttcatcacg
     4201 ttcagcctga acactggtca gatgataacc tccatccact tcaataaccc ctaatggagt
     4261 cttcccgacc ttatagtaaa caacaaagtc acaactggcc cggttacgca tgaataactg
     4321 ttcacggtgt gtaaaatccc cccgctccag caacaccaac tgattcagcg ctatttgtga
     4381 atgaaacatc atactccgat aactatcctg cgacaggata tcccgcaaca ggcaggccac
     4441 aatctgttct gacttaaagt gtgaatcatt tgaattcaat ctactattca gtcgttccag
     4501 tgatttgtca tactcactgt acagcaaatc aaatgctgat atgaccgggc tctcgaaaat
     4561 ctctccgtca tcagcgtagt atttgatgta acgaatcagc gccgcaatat gtccagcgtg
     4621 gcgttcaaat acatcattac cagtcaccag cgtaaattta tttcgggccc ttgaaacagc
     4681 gacattcacc agttcaggat tatcaacaaa cgcgatgttc cgactgtgct ggctgctgcg
     4741 cttcttatcc agcacagtgg aaaacacaat ttcatcgcac tcccgtccct gaaatttatg
     4801 caccgtcgat ttcacaaaat cagcaggcag cactttttca gccagattaa cctgtgcatt
     4861 atatggggca atgtatcccc tgctactttc ttcatcccag taatgacctt ccagagattc
     4921 aagctccctg agatttgaaa aattacgggt atggtttcct tttgccgtga tgaccagaga
     4981 taatgaggct tcaccggaat caaccgttaa cggtatcaga gcattatcat aaaactgctt
     5041 attacaaaat tgaatgattt taggatgaca acgataatgc tctttaagaa gggttactgg
     5101 caccatgttc ctgaacaaca tacaaaccga atcaagtaga ctatatttct cacaattata
     5161 gtattctgcc ggtggagatg ggctgttcgg gagtaataca ggaacatgtg gaagttgctt
     5221 acgatcccct acaacgatta cattacgggc acatcccagt cccagaatgc ccggaacaat
     5281 atcctgctgc gaggcttcat caataataac ataatcaaga agggccccct tgccgataga
     5341 gttaataatc gaatgcgtac tgctaccaat aatcggaaaa cgctttataa aacggtcaaa
     5401 ttcatccctg tatgtctctg ccgagaatga acttcttgtt gaaacatttc ttcgcagatg
     5461 ttgctttaaa taaagcatag accatgatgt caggcgcccc agtaaaattt taaaatcaga
     5521 ttttttaaat acttcctcat attcattcag agaaatttgt ttttcccgga tatttttttc
     5581 ataataagaa agctgaagag aagtaaatag cgccagcctt ctctccggaa tcatcaatgg
     5641 tttgactttc agtatcatga agtttaacaa caaatcaatt ctgtttctga atccaatccg
     5701 cctgtcagaa aggtaatgaa tataagccat caggtcggtt gttttttgtg aggaaaatcg
     5761 gtatttgtga gaaaaaagct cctccggtcg aagattctga ctctgccatt gctgcaggta
     5821 tttatactca atattcagct cattaatttc tgcttttaac gaagcgacct ggttaatggc
     5881 attcaggtgt tttttcactt gttgaagtac atcatcaatc gcatttgcag acggtgagtc
     5941 aggtaagact tcctcagaac gcgaaatcga cgttgaaaaa aactgttgcc tgttctccgt
     6001 actccccagt cgtgccatca catatcccag ttgctgtttg tccatctttt cataaacatt
     6061 actgacagct gagttattat tggacaaaat tgccactgtt ttattctgaa ttaatatatt
     6121 agcaacaata ttgagtatag tctgtgtttt acctgttcca ggtggtcctt caataactga
     6181 aatctgtgag gaaaatgccc gtttgacagc cagcaactga ctttcattaa gaccaaacgg
     6241 aaaaatcaaa ccccatggcg aatcacgttt tttactctgt cctgaacagt atgcatgcaa
     6301 ggcggtatcc ggatgaggaa ctattttctt tatctgccta aggatattct catcgatatt
     6361 tctgttatta ccacgggcat aaaaaatacg ttcattagcc accctacaca agtaattaaa
     6421 cacggcatgc tctgtaaatg ctgaacactg ttgaaagtca aggccggtcg ttttcatgat
     6481 gtagggtcgt tcaccctcgc ggtaataaac agcagcatat ttatcaccat atataataac
     6541 cagttcagcc cgggatgtta cagcgttccc tttcctttcg aggaaaacat ttcgcaaatt
     6601 tacagttggc gttatctgac atacagaaag cggacgagta tattttctcc cgctgcgaaa
     6661 atgacaggtc agcataacct ctctgtcgtc atttccagcc cacagcgtcc agtcggtaat
     6721 tttatgagtc ttattttcac cgtcaacaca aatcgtgacc atattctcac tcagaaatta
     6781 tcatttaact tacggaaaat tgttaaggca ctcccgatat tagctccgga ggattttcca
     6841 caactccggg actgttaaca tatttctgcc tgccatatat acattgaatt tagtcataac
     6901 agtcaatacc agaattacag gaatgcgatg ccgatacgct ggttattgaa tactggtagc
     6961 ccctgagcac taattcatca cgatactctg tatttactgt tttcactgct ttttacaacc
     7021 attcccccag gtaaaatgct gctcctccgg tgatggctgt gtcagaaact gccatcccgg
     7081 aatagccacg ctgaccgcca gcaatgccgc acagacacca gccacaaacg gtttcagaaa
     7141 cgtgggcggg gatttctgaa gggagggtga ctgctccgcc tgcactttcc ccttgcgatt
     7201 atcaaggctc agtgcatcca gacgcaccgc gccatttgcc gcaagcagcc cttcccggtt
     7261 atccacgctt cccggcagac tcagcgaaaa atcccctgtt cccgtctgaa tcagactgcc
     7321 tttacgattt gacaggcttt gtgccgatat ctgcagtgtg tcggcattta tcgtaccgcc
     7381 attactgctg aactgcccgg cagtccgggc ggaaagcgta ccggcactca gttgcgcatt
     7441 ctgtgtagac agatttccac tggttgcatt cagctcagta tgttgtccct gtatccggct
     7501 gttacctaaa tccagcccct gctcaatgtc ccgtaaatcc agcagagaac cttcgtgtgc
     7561 cgggaatgag gaatacaact gaatatagtc atcactgtcc ggtgtcagac gcacatgtcg
     7621 cacaacaccg ggaataatga ccagccggag gataccgctt tttaaatcct gcgaaggtgc
     7681 cagaacacgg gtggtgacat aaccatgatc gaccagacgg ttctgcaatg tactcatcag
     7741 cagattaatt cctttcgccc ccaggcaatg cccgaccgcc ccgttggcga ttttttgtaa
     7801 aggcagccag tgtggtaacg catcagcccc cttcagttcc gtctgtttaa tctgaaaaca
     7861 gggcgtttca acaggaaaat taatcttatg ggcaaaagag ccaggtgcag aaaggcgaac
     7921 atcaggggct gaaggcgtca gttgttgctc aagggcttcc tgacgctgct gctgaatgat
     7981 aaatgcttca tctccgctac gccggacatc cgctgcctga agagataaag gcgataataa
     8041 agcagacatc agtgtcagtg acagcaagga cggacagcgc cggaataagc tttgttcctc
     8101 agtcctctcg ttcctgcaac ataattttcc agtcaaacga aaaacctttt attcatgtca
     8161 taccaacaat caccagcctc ctgacgatac ctcccaccat gttttgcgat ggcacgctac
     8221 cgcccttccg gtatttaccc gcatcaaccg gtaccagaat gctgagtaac aaaccagcca
     8281 gaattgactt aattttgatc attatagaat aatgatctga actacagcaa catgacagat
     8341 ttatcgatcg atataacaca aatgaacagt tctttattct gccatatatc gcaaaaacag
     8401 cacattacaa aatttataac cataattact ggaaaagtgc agaaaattat tccactgaat
     8461 gcttttaata cagttggtta gcttaaaaaa tagcaatttt tgatttcagt cattgaaagt
     8521 tgcaatgttt gacgattcaa cagtatttaa gtgagtttca ggatatggga aggacatgaa
     8581 aggaggagcg caataaaata cacaggaaga aggggaaaat gtttcgtaaa aacagaaaat
     8641 taacagcgca catcaggaaa cgcagtctcc tgattcagtg ctaccacatg aaaatttagt
     8701 taactgttac cgtactaaaa tttatgtcag aagatgttcc attaccgaaa caaagacgat
     8761 catggtgcgc ttgtcaccct gaccagtgtt gaggaaaccc gggtggtgtt tatgcgggac
     8821 ggctatccgc atccctgcat gcaaccgatg tacaactttc tgggaaaatt caaacccgaa
     8881 ccccgggaag aaacagaata aaacgaaatc cctgccggca atcacactac ctgatagcag
     8941 cataatccac ttattaattc tcatccccgg agacaagccc agaaaaccac tccgggtgtt
     9001 cactgtatgc cgtgttcagc gcatccctgg aaacgtttat ataaaagctg tcctgcaatt
     9061 tgcgggtaac aacttctgcc aggcgattcc ggttatccac cagtccggca taacctctct
     9121 ctgccagcca ctcattcagt tcatgttcca gagacagatt gataatatca taatcggacc
     9181 ttgccattcc cgtttcctta gcacatgttt tcaggcacgg ggataaagta taaagaatgt
     9241 catactgctg ctgccagagc aatattcccc ctgatggttg tatcagagat ggtatgccgg
     9301 tactttgcgg gttgttcccg tcaggttatc gtgcacctcc acggtcgtgg tcaccaccgg
     9361 cattccggcc tttctcagcc tcagaacatc agccgcaata cgctgactgc caaaccagaa
     9421 caggccgtcc agtgcagtca ccagcaaacc cgcctccagt gcatacttca gccgttcacg
     9481 gggcgctttc acttcccggg caacctgctg atatggcaat gatgtgtttt cattcccaac
     9541 cacgcgacga atacggtgag acagatgata ccggtatgta tccggcacac cagaaaggct
     9601 ggccttcagg ctgtacacgc agccaaaccg tttatcgtta aacaccacat ttttctggct
     9661 gatgccccat tcttcacgca gcgcggcaat caattgcggt gtacgggtaa gcagcaagcg
     9721 aaaaggcagt tcaaaactgg tgacgtaatc cacattcagc agtgcaatac gcaaccgttc
     9781 ttctggcccg gcctctgtct gccggcactc ctccagaaca tcctgccact gccggcgaag
     9841 acggggggat tcgttccgtt ctgtaaaaca gtatttatcc gccagatagt caattcgtgt
     9901 acgcatactg aaaggaattc cgtataaaga ttcagcagac aaaactttat cagccagtaa
     9961 aaattaacgg aagcattgat attcctcccg acaatcaccg gatgattgtt gcaataacct
    10021 gcgacataaa ggactgaaca gccgttttta acgccacgta ttgccctgat gaaccaggcc
    10081 gggcaacccg aaaacatctt tccacccggc attgtccgcc agcacttatc accgaccagg
    10141 tctgttgcag taaatccacc agttgcgcac attcttcatc agccatgacg ccgactcgcg
    10201 gatggcaaat actcgtctgg ctgagcagca acagacatcg tatctgtgct ccttcatgtt
    10261 gttcccgcaa ccagcgtaat accaccggcg ctgatgggct gaccgtgtga ttatgctccg
    10321 taatacgttc gtgtacccgt cggtgaaagg aactacaaaa tgtctgaatc tgttgaccat
    10381 agattccttc tgccgaatag agcgtgaagt gaaatgccaa cagatttggc cagtggtcca
    10441 ccgcctgctg aacaaaacgc cggatttccc ccggctctga aattaaggct tcggttattt
    10501 gcacgaaatg ttccttttca acccctgttg aattttgtca agccggtgca gacacagtaa
    10561 agcgcgcatc aacacctgaa aggtacgata caaacacaag ttacaaacaa taagaacgga
    10621 aggacaatta atcagtgagg aataataact aaattaacca gaagaatgtt actgacaata
    10681 ctaccaatac ttaaatagca atatattaat taatgtaaga acagatgccc ggtaagacat
    10741 atacacaata catccacaaa cacactcttt tcattaaatc aatcacctga ttaaaccatg
    10801 aaagtgtgat gtaagtcaaa attactgata ctatcaattg ataatgatta tctctatcat
    10861 tatcgtgaaa ttcgagaatg aaaggagaag tagtcattat aagcctttta taaatcgtca
    10921 tcatcatagt aaagtagaac actttttata tgagttacta tatgaaaata tactggacaa
    10981 gaaaacgtat tcctgaactt tcagcacttc caccttcatt aagaaagaaa aattttactg
    11041 acgcatataa tgcagcatct tctcatatcg aatactggat aggtgctggc gtctcattta
    11101 tctcgatgat gattttatcc agagtatatg actttctttt acccgcccaa gatactttcc
    11161 cgggtgatat catcagaagt ttatgtgttg tgtgtccatc catattaatc tggtttcagt
    11221 tttcggtata tgtcatgagg aaatattaca gacatattct ggtgcgagga aaagaaaccg
    11281 aaaccatttc agaaaggttg atacgtgagg ctgatacacg agaatatgaa ctatggcgtc
    11341 cggtccgtcg ctttttctcc attgttttcc tcattgttct attaggttgc attcattcac
    11401 tgataacaac tataaaataa aagttatggc tgttctgata caggaaaata aacgttcatt
    11461 ctatggttac ctcttcctgt tatatagaag aattttaaac gacaagtaag ggaatataaa
    11521 tgaaaataac aaaaaataaa aatgcaacta taaaaaacag tcatcagtcg gctgcttttc
    11581 aatgtttacc ttatc
//
LOCUS       DAJTNM010000119        11518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTNM010000119 DAJTNM010000000
VERSION     DAJTNM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.119"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..962
                     /locus_tag="OO172_003321"
     CDS             <1..962
                     /locus_tag="OO172_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5513391.1"
                     /translation="NRYESVARTMVPKIGGGRLVSSVTKEELLYIRKDLLTGYQNSTK
                     NKAAAKGRSVVTVNYYMTTIAGMFQFAADHGYLEANPFQGIKPLKRARAEPDPLTRDE
                     FIRLIDACRHQQTKNLWSLAVYTGMRHGELVSLAWEDIDMKAGTITIRRNYTKLGEFT
                     LPKTEASTNRVVHLIQPAISVLKNQAEMTRLGKQHHIKVQLREYGRSVNHECTFVFNP
                     QVVRKSKQVGFIYKVDSIGDSWETAIKRAGIRHRKAYQSRHTYACWSLSAGANPSFIA
                     SQMGHASAQMVFNVYGAWMTDSNAEQIAMLNQKLADYVPWSPK"
     gene            975..1745
                     /locus_tag="OO172_003322"
     CDS             975..1745
                     /locus_tag="OO172_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5513392.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            1798..2193
                     /locus_tag="OO172_003323"
     CDS             1798..2193
                     /locus_tag="OO172_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5513393.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2234..2977
                     /gene="cmoA"
                     /locus_tag="OO172_003324"
     CDS             2234..2977
                     /gene="cmoA"
                     /locus_tag="OO172_003324"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5513394.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2974..3945
                     /gene="cmoB"
                     /locus_tag="OO172_003325"
     CDS             2974..3945
                     /gene="cmoB"
                     /locus_tag="OO172_003325"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5513395.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4110..6539)
                     /gene="torZ"
                     /locus_tag="OO172_003326"
     CDS             complement(4110..6539)
                     /gene="torZ"
                     /locus_tag="OO172_003326"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5513396.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMAAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6564..7664)
                     /locus_tag="OO172_003327"
     CDS             complement(6564..7664)
                     /locus_tag="OO172_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5513397.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8052..8798)
                     /gene="cutC"
                     /locus_tag="OO172_003328"
     CDS             complement(8052..8798)
                     /gene="cutC"
                     /locus_tag="OO172_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5513398.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(8812..9378)
                     /locus_tag="OO172_003329"
     CDS             complement(8812..9378)
                     /locus_tag="OO172_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5513399.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9594..11327
                     /gene="argS"
                     /locus_tag="OO172_003330"
     CDS             9594..11327
                     /gene="argS"
                     /locus_tag="OO172_003330"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5513400.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
BASE COUNT         2816 a         2944 c         2802 g         2956 t
ORIGIN      
        1 tcaatcgcta tgaatccgtt gcaagaacga tggttccgaa aattggaggc ggcagactgg
       61 tgtcatcggt aaccaaagag gaattgctgt atatcaggaa agatttgctg accgggtatc
      121 agaattcaac gaaaaacaaa gcagcagcaa aaggacggag cgtcgttact gtaaattatt
      181 acatgacgac aatcgctgga atgtttcagt ttgctgcaga tcacggttac ttagaagcaa
      241 atcccttcca gggaattaag cctcttaaaa gagccagggc agagccagat ccgttaactc
      301 gtgacgaatt tattcgcctg atagatgctt gccgacatca gcagacgaaa aacctgtggt
      361 cattggctgt gtacacagga atgcgtcacg gtgaactggt ctccctggcc tgggaagata
      421 tcgatatgaa agcaggaact attactatca ggcgcaatta tacgaaactc ggtgagttca
      481 ctctacctaa aactgaagca agtacaaaca gggttgtgca tcttatccag cccgctatca
      541 gtgtcctgaa aaatcaggct gaaatgacaa gactgggtaa gcagcaccac atcaaggttc
      601 aactacgtga atatggacgt tcagtgaatc atgaatgtac tttcgtattt aacccccagg
      661 tggttagaaa aagcaaacag gtaggtttta tctacaaggt agattccatt ggcgactcat
      721 gggaaacagc cattaagcgt gcgggcatca ggcaccggaa agcataccag tcacgacaca
      781 cttatgcgtg ctggtcatta tctgccggag caaacccaag cttcattgcc agccagatgg
      841 gccatgcaag tgcccagatg gtgttcaatg tatacggagc atggatgact gacagtaatg
      901 cagaacagat cgcaatgctg aatcagaagc tggcagatta tgtcccatgg tcaccaaagt
      961 gacacaagag acttattaaa atcagtgggt tagccttcaa tacccgtcat gttaactgcg
     1021 tggagggcaa caccacgctt tacgccctgc cgaaacccga ggttgtcctg cgctggcgtg
     1081 aacagaccac agatgacttc cgcttctgtt ttaagtttcc ggcgaccatt tcgcatcagg
     1141 cagcattacg acattgcgat gatttagtga ctgaattttt gacccgcatg tcaccgttgg
     1201 ctccgcgcat tgggcaatac tggctgcaac tgcctgccac attcggccca cgggagctgc
     1261 ctgcgctttg gcattttctc gattctctcc ctggcgaatt taattatggt gtggaagtcc
     1321 gccatccaca gtttttcgcc aaaggggaag aggaacaaac gcttaatcgc ggtttacatc
     1381 agcgcggcgt taatcgggtg attttagaca gccgcccggt tcatgcatca cgtccacaca
     1441 gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt tccggtacat gctgtactga
     1501 cggcgacaaa tccgctgatc cgttttatcg gtagtgatga tatgacgcaa aaccgggaat
     1561 tatttcaggt ctggttacaa aaattagcgc agtggcatca gaccactacg ccttatcttt
     1621 ttttacatac gccagatatt gcccaggccc cggaactggt acataccctg tgggaagact
     1681 tacgtaaaac gcttccagag atcggagcag ttccggctat tccacagcaa tcttctcttt
     1741 tctgaatttg ccacctatca tagacaggtg ccatcggcca ttttaaaggg agtttgtatg
     1801 gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa tgaagttctc ttttgatgtc
     1861 gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg gtggttttag cgaactgcaa
     1921 agcgctattc gcattcatgg taacgcggtg gaatatattc ccatcgcgat tgtgctgatg
     1981 ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc atatttgcgg catcgttttg
     2041 cttgctggtc gtctgatgca ttattacggt tttcatcacc gtctgtttcg ctggcgacgt
     2101 tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg tgctggcgaa tctttggtat
     2161 atgccctggg agttggtttt ctccctgcgt tagcgcacaa tacgccactt tctttttccc
     2221 ggatttttac gttatgtctc accgcgacac gctattttct gcccctatcg ccagactggg
     2281 cgactggacc tttgatgaac gggtagctga agtcttcccg gatatgatcc agcgttccgt
     2341 tcccggctat tccaatatta tttccatgat tggtatgtta gccgagcgct tcgttcaacc
     2401 tggtacgcag gtttacgatc tgggttgttc tctgggcgcg gcgacgctct cggtgcgtcg
     2461 caacattcat catgataatt gcaaaattat tgccatcgac aactccccgg cgatgattga
     2521 acgctgccgt cgtcatatta acgcctataa agcccctacg ccagtagacg tcattgaagg
     2581 tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg gtgctgaatt ttaccctaca
     2641 attcctggaa ccttccgagc gccaggcatt actggataaa atttatcaag gactgaaccc
     2701 gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa gatgccaaag ttggtgaact
     2761 gttgttcaac atgcaccacg actttaaacg tgccaacggt tacagcgaac tggagatcag
     2821 ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat tccgtggaaa cccataaagc
     2881 acgcctgcat aaagccggtt ttgagcatag cgagctgtgg ttccagtgct ttaactttgg
     2941 ttcactggtg gcattaaagg cagaggacgc tgcatgatcg actttggtaa cttttattct
     3001 ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc tgcccgcgca gattgctaac
     3061 tggcagcgcg agcagcagca cgggctgttt aagcagtggt ccaacacggt ggaatttctg
     3121 cctgaaatta aaccgtatcg tctggattta ttgcatagcg taaccgccga aagcgaagag
     3181 ccactgagca ccgggcaaat taaacgcatt gaaacgctta tgcggaacct gatgccatgg
     3241 cgcaaagggc cgttctcact gtatggcgtc aacatcgata ccgaatggcg ttccgactgg
     3301 aaatgggatc gtgttctgcc ccatctttct gatttaaccg gacgcaccat tctggatgtc
     3361 ggctgcggca gcggttatca catgtggcgc atgattggcg caggcgcgca cctcgcggtg
     3421 gggatcgacc ctacgcaact gtttctgtgc cagtttgaag cggtgcgtaa actactgggt
     3481 aacgatcagc gcgcacattt gttaccgtta ggtattgaac agcttccggc actgaaagcc
     3541 tttgataccg tcttttcgat gggcgtgctt taccatcgcc gttcgccgct ggagcatctc
     3601 tggcagttga aagatcaact ggtgaatgag ggtgaactgg tgctggaaac gctggttatt
     3661 gatggcgatg agaacacggt gctggtacca ggcgatcgtt acgcgcaaat gcgtaatgtc
     3721 tatttcatcc cttccgcgct ggcgctgaaa aactggctga agaagtgtgg ttttgtagat
     3781 atccgcattg tggatgtgtg cgttaccact actgaagagc agcgacgcac tgaatggatg
     3841 gttaccgaat ctctgtccga tttcctcgac ccacatgatc cgagtaagac agttgaaggt
     3901 tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc cgtaaaggtc tggtattact
     3961 gccggatgcg gcgtgaacgc cttatccggc ctacaaaccc ttgctaattc aatctattgc
     4021 agggactatg taggcctgat aagcatagcg catcaggcag ttttacgttt gcataacctc
     4081 agcgcccgtt tccgggcgct attcacttct tacgcctgaa cagcaggctt atcaaatgcc
     4141 gttaacttcg gtgcgttacc cgtatatttt tcgatataca ccagcgcaga gttaccggca
     4201 caggcatttg ccagctgcga gctggggata tccgccgtta acacgttcgc actgccgttt
     4261 ttacacaagc cgttttccag atctggccat gcaccttcat gcacgcatac cacgcctttt
     4321 ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac cacgtttgtt ccagacacgc
     4381 accagatcgc cattcgcaat accaaatcga gcagcatctt cggtgtgaat agtgatcggt
     4441 tcacgatcgg caaccgcata ttttttacgc agttccgcat agttaagctg actgtgtaaa
     4501 cggtgtgccg gatgtgcggt cagaagctgc aactgcttct cgtcggcggt acccttccac
     4561 tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct tatagccaaa tttttccagc
     4621 gttttggagt aaatctcaat tttgccgctt ggcgtaccca gcgcattttt caccggatcg
     4681 gcgcggaaat caccataacg aacgtactgt tcgttcttct cgctgcggcg catttcgatc
     4741 agtttatttt gctgccagaa ggcattaaac attggcatag tgacgcgttg cgcacgggcc
     4801 cctttctgag cggcatcata gaaaaatttc agccacgcca tttcatcttt accttcggta
     4861 tagatctctt ttccgccagg tttgagtaat tccgcaagat cggcaaacac gtcaaaatcg
     4921 ttacgcgctt caaattgcgg agcaacagcc tgcttcatcg gcacaatatg ctggttgctg
     4981 taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg tggtgatcgg taatacgata
     5041 tctgcgtgtt tggctgctgc ggtccagtag cattcagaaa cgacgatcat ctccggtttc
     5101 tgccaggctt tgatcagacg attagtatcc tggtgatggg taaagttacc gccacccgcc
     5161 caccagatca ttttgatatt cggatacgtt tgttctttac cgttatgctg atatttgccg
     5221 ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa tcgccgccat tccgccatca
     5281 tcagcggcct cgctggcctg tccggcaatc gccgcagaca tttccggcag cacgccgcca
     5341 acacgcatcg ggttgccacc attggagtaa tgataagaga aaccaaaacc gccgcccggt
     5401 gtaccaatct gccctaacat cgccgccagc gtcaccagca tccagtgttt ctgttcacca
     5461 tattgctggc gctgaattcc ccagcctgcc atcagaattg tgcgattcgc cgccatcagt
     5521 tcagcgagtt tgactatctg cgcttccggt acgccagtaa tttccgccgc ccagaccgcg
     5581 ctcttcggcg tattatcgct cttacctgtc agatactctt caaactgcgg atacccggta
     5641 gtgtattttt caaggaacac tttatcgtgt ttgccttgtg tcatcagggt atgcgcaatc
     5701 cctaacatca gtgccacgtc ggtgcccata ttcggcgcga tccaggtggc attatcgcca
     5761 aagaattcga tggtttcgga gcggatagga tcaatggcaa tcactggttt gccagatttt
     5821 ttcagctgat ggaagtattc cagcccttgc tcatcggtac tgctccaggc aatttttaag
     5881 gtattcagcg ggttcattcc ccacagcacc acaacctggc tgttttccag aatcagcggc
     5941 caggaggcct gctgttcata cacctctaca gaaccgacca catgcggcat gatcacctgt
     6001 gctgccccgg ttgaataatc gccgctatgc ccggaataac cgcccgccag gttcatataa
     6061 cgttgcagta aggtttgcgc tttatgcaac acgccagaag agcgccagcc gtaagatccg
     6121 gcaaaaatgg ccgatggtcc gttagcttta cgaatacgct catgttgctc atgaatcagt
     6181 tttaatgcct gttcccagct cacctgtaca taggtatctt cgccacgacc tttcgccggt
     6241 tgcagtggat tgtcgagata gctttttctc accatcggat gctgaatacg cgccgtggtg
     6301 tgtacctgat ccgccgccgt agactgtaag gagttcggta tggttttcgc cagcgcgcct
     6361 gtcgaagaaa caatcttgcc gtccttcact tctacgctca tcgctcccca acgtcccgcg
     6421 gtgaggattt taccgccctt ctcttctgcc catgcgggga gcggtgctgc cgatgtcacc
     6481 accagcgctc cagcggcaat accgctgtgt ttaataaatt cacgtcttgt taatgccata
     6541 acttcctccc tgatcaacga ggatcactgt ttctcggtaa tatctttggc gttgtactgg
     6601 aaataccgcg ttaaaatgtc cagttcgttt tcgctcatgc tggttcgtgc ccccattcct
     6661 ttggcaatgg acggccacgc attgacggtg taatggtcgg cggcaataga ggcatgacaa
     6721 ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc atagcggttt acggtccgct
     6781 aatgcgggat cggtaagcgc accctgtaaa gacgcctgac gccattgatt gccgtattcg
     6841 tcagcctgcc attccccgtt taccgtgagc gccttgatac cttcttcact taatgtggct
     6901 agcgccagcc gttgacctgc cgccaggtag agcgtgtttt cactgccctg catttgataa
     6961 ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa cgacggtgag atcggtacca
     7021 ggattcacgg tagccagctc gcctatgcga gaagttttga aaggataaat atgtgcgcca
     7081 ttagtcactg aagtagcggc ctgactttcc agctcatgcg ccgcgttgtc atccattttt
     7141 atttctggcg gaaaatgggc aatgccttta tgacaatcga tacaggtttc gccgcccttt
     7201 tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg aggcaatatc catggcatca
     7261 aaagaatggc aactacggca cgttgcagag tcagttgctt ttaattcttt ccatactgtt
     7321 tcggccattt cctggcgatg agtttcgaac ttatcgtcac tgtctatttt gccgctaaca
     7381 aattcatgat aaatatcttt agatgctttt aatttagcaa ataaataatc catccctgac
     7441 tttggaatat ggcaatcggc acattccgca cgtatccctt tctggttcga aaagtggaca
     7501 gttccctgat attcctcaaa aggtttactc atcgagtggc aggaaaggca aaatgctgta
     7561 tccgacgttt tatgtaaggc tttttgcgcc agcaataacc cgccgccacc aaccacaacc
     7621 gctatcagca aaaacaataa cccaatgcgt tttttccctc gcatagattc ctctaaataa
     7681 attccagaac accgaaatca ttgtataatt aaatacacaa cgaattaacc gcttgcataa
     7741 ctcggcacaa cactgcctga aataatcgat aaagaatatg atttattaca atgtaatcat
     7801 taattgctaa ggaataaccc agttgctatt tagaatatat atttatcagt tttagcaatt
     7861 taaatcccat aatcaatgtg aatataaaaa atatttttag cacaacgcta tgcgcactgt
     7921 cacccgctca acagatcacg gttatcgttc gttttttata ctgctcaggg ataaaaaaag
     7981 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
     8041 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
     8101 gccccgtcta cgatataacg cgaatactcg tccgcgtgtt catctgatga catggacaat
     8161 ccttgattac gataacgcat cggtgaggct tgccacgctc ccgcggagct atggacttcc
     8221 agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc tccagccata
     8281 atgattggag catcacgatg ggcaataagt tccataattt ttgataaacc ttgcagtgcg
     8341 tctgattttt gccctgatgt cagtacccgg gcaatgccca attccgcaag attattgagt
     8401 gtatataaag ggttagcgca catatcgaag gcgcgatgaa aagtcactgc cagcggaccg
     8461 gcagcagcca ttattttttc cattcgtggc atatcgacat tcccatcaac atcgagaacg
     8521 cccgtcacca gtccaggaaa acctaattcg cggaccgtgc gcacatcctc aagaatggcg
     8581 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaattat cggatgcaca
     8641 gggatcgtca cccgctggcg cacggatttc agtcccccca acgacggcgt taagcccccc
     8701 tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca
     8761 cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta attacgctga
     8821 ctgctcgctg gcaacgatct cttcaatgga ccacggatga aacttaatag ttgttttgcc
     8881 atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg gtgaactggt
     8941 tttaactgag atccccggta ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     9001 attcagcgtt tctggatcac ccggcagaac gatttcaata tgttcccaac cttcgtgtgg
     9061 gtaacgtttt tccccaggcc acggtagctc cacattggaa aactgccagt gcgcaacctg
     9121 taccggttca tgcaatttaa acagacaaat cggtctgcca ttgatcattt tttctgacaa
     9181 aagctcgcca cactgttcaa aaccgcgacg ccagcgctca gcggtgacgt tttgatggca
     9241 acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc aaccccagac ggcgggaaag
     9301 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca gctcgtcgat
     9361 agattgccag ttcgccataa tcactcttcg tctttcagta aaagcgttaa tttaccctgt
     9421 tgccctgtgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt gaaaacggca
     9481 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     9541 tctttaactg gtgcgggcaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     9601 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     9661 attgcgaacc gcagattcgt cagtcagcaa aagttcagtt cggcgactat caggctaacg
     9721 gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca gagcaggtgc
     9781 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgccggt ccaggcttta
     9841 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     9901 atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac tctgcgccaa
     9961 acgtggcgaa agagatgcac gtcggtcacc tgcgctctac cattattggt gacgcagcag
    10021 tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg
    10081 gcactcagtt cggtatgctg attgcatggc tggaaaagca gcagcaggaa aacgccggtg
    10141 aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat tacgacgaag
    10201 atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat
    10261 atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac caaatcactt
    10321 acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggcgaaagc ctctacaacc
    10381 caatgctgcc aggaattgtg gcggatctca aagccaaagg tctggcagta gaaagcgaag
    10441 gggcgaccgt cgtattcctt gatgagttta aaaacaagga aggcgaaccg atgggcgtga
    10501 tcattcagaa gaaagatggc ggctatctct acaccaccac tgatatcgcc tgtgcgaaat
    10561 atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc cgtcagcatc
    10621 aacacctgat gcaggcatgg gcgatcgtcc gtaaagcagg ctatgtaccg gaatccgtac
    10681 cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga cggcaaaccg ttcaaaaccc
    10741 gcgcgggtgg tacagtgaaa ctggcagacc tgctggatga agccctggag cgtgcacgtc
    10801 gtctggtggc agaaaagaac ccggatatgc cagctgacga gctggaaaaa ctggctaacg
    10861 cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc acggactaca
    10921 tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac atgcagtatg
    10981 cttacacgcg tgtattgtcc gtgttccgta aagcagaaat taacgaagag caactggctg
    11041 cagctccggt aatcatccgt gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt
    11101 ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg tgtgcttacc
    11161 tgtacgatct ggccggtctg ttctctggct tctacgagca ctgcccgatc ctcagcgcag
    11221 aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacagcg aagacgctga
    11281 agctgggtct ggatacgctg ggtattgaga ctgtagagcg tatgtaatcg atttttcgtg
    11341 agagtgaagc ctgatcaggg ttagccgatc aggctttttt attgccatct aaatgtattc
    11401 tgaaaacgga catgccattg ttttctcact gttgagtaag aggccagaag cgtaatatcc
    11461 aggcccagga aaacgataac tgttcaattt aaggaatacc gaagtgttta aatttctt
//
LOCUS       DAJTNM010000120        11458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTNM010000120 DAJTNM010000000
VERSION     DAJTNM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.120"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..448)
                     /locus_tag="OO172_003331"
     CDS             complement(<1..448)
                     /locus_tag="OO172_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5513401.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPI"
     gene            577..912
                     /locus_tag="OO172_003332"
     CDS             577..912
                     /locus_tag="OO172_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5513402.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPIFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(909..1631)
                     /gene="folM"
                     /locus_tag="OO172_003333"
     CDS             complement(909..1631)
                     /gene="folM"
                     /locus_tag="OO172_003333"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5513403.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(1668..3050)
                     /locus_tag="OO172_003334"
     CDS             complement(1668..3050)
                     /locus_tag="OO172_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5513404.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(3236..4180)
                     /locus_tag="OO172_003335"
     CDS             complement(3236..4180)
                     /locus_tag="OO172_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5513405.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            4704..6236
                     /gene="pntA"
                     /locus_tag="OO172_003336"
     CDS             4704..6236
                     /gene="pntA"
                     /locus_tag="OO172_003336"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5513406.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            6247..7635
                     /gene="pntB"
                     /locus_tag="OO172_003337"
     CDS             6247..7635
                     /gene="pntB"
                     /locus_tag="OO172_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5513407.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(7660..8694)
                     /gene="tqsA"
                     /locus_tag="OO172_003338"
     CDS             complement(7660..8694)
                     /gene="tqsA"
                     /locus_tag="OO172_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5513408.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            9105..9470
                     /gene="mdtJ"
                     /locus_tag="OO172_003339"
     CDS             9105..9470
                     /gene="mdtJ"
                     /locus_tag="OO172_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5513409.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            9457..9786
                     /gene="mdtI"
                     /locus_tag="OO172_003340"
     CDS             9457..9786
                     /gene="mdtI"
                     /locus_tag="OO172_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5513410.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(9825..10646)
                     /locus_tag="OO172_003341"
     CDS             complement(9825..10646)
                     /locus_tag="OO172_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5513411.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(10746..10829)
                     /locus_tag="OO172_003342"
     CDS             complement(10746..10829)
                     /locus_tag="OO172_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513412.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(10922..11257)
                     /locus_tag="OO172_003343"
     CDS             complement(10922..11257)
                     /locus_tag="OO172_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5513413.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
BASE COUNT         2863 a         2759 c         2941 g         2895 t
ORIGIN      
        1 tgatggggtc gatagttaac gtgccgaaat gcagggcttt gtagggagtc agagacgttt
       61 cctgaagacc tttggtcagt gtggcttgct cattctgacg caaatgcaaa cgtaaacgcg
      121 ctagcaaaac agcagggggc gtcgttttga gaatatagtc gcaggcaccc atttccagtg
      181 ccaggatgtg gttcatatcg ctatcgagag aggttagaag aacaatcggt ccagaccact
      241 ttgcgcgtaa atcacgacaa atggtcatgc cgtccttgcc tggtagcatg atgtcgagta
      301 acaccaaatc cggattttct cgcaaaatgg tttcttcggc ctggtcgccg cgcggttcta
      361 cggtaacctg catatcatgt tttgccaggt acgcggcaat cagtgaaccg acttccgcat
      421 catcttccac aaatacgata gtgttcataa cattcacggt agatataaaa acgtcacaat
      481 acaccgcgct gtttttactt actatcaatc gtttctaaac aagttttcat caggtattct
      541 actgatgact tgttatggaa gtgttaaggt aaaaagatgg ggctggtaat caaagccgcg
      601 ctcggtgcgc tggtagtgct gttgattggt gttttagcaa aaacgaaaaa ttattatatc
      661 gccgggctga ttccactttt cccgatcttt gcgcttatcg cgcattatat tgttgccagc
      721 gaacgcggca ttgaagcctt acgcgcaacc atcattttta gtatgtggtc gattattccc
      781 tattttgttt acctggtgtc gctgtggtat ttcaccggaa tgatgcgctt gcccgccgct
      841 tttgttggtt ctgttgcgtg ttgggggata agcgcatggg tgttgattat atgctggata
      901 aagctgcatt aacgcagatg acgaccgcca tcaagtggga aactgcgtcc ggtgacaaag
      961 caactggtaa gtaagtaatc gaccaggtcg atcacttctt tctcgccagg cgcggttttc
     1021 atcagtgatt tattcagcgc ctgttgtcga tattcggcat catcatgttc attaaacagg
     1081 atcagcgatg gcgcaataga attcactttc acttccggtg ccagcttgcg ggcaaacgag
     1141 cgggtcatat tatccagtgc cgctttgctt gcagcatacg caacatgttt gtcgctgccg
     1201 cgctccacca cataatcggt aaagtgaatg atatcgctgg cggcgtgtcc gtgcccacgc
     1261 agtaatcttt ccagcgcatg gttgagcagg tatggggtat taacgtggat ctgcatcatg
     1321 caagccagta cgtcggccag tggcgcaccc ggtttttccg caatccacgc actggcgtta
     1381 tgcaaaatag cacgcagacc atgggtggtt tttagtactt catcggcaaa cgccatcaca
     1441 ccgtcgttgg tcgaaaaatc agcctgaata cactgcgcac ctgcattaat cagtccatca
     1501 atggctggat agtgtgtccg atagctgaca atcaccggtt gcttttgatt aatgaaatgc
     1561 catgcgaggg cgaggccgat gcgacgacct ccgccagtaa ttaatattgg caagggctgg
     1621 gttttaccca tcgttatctc ctttgctatc caacgatggg atgaacgtta tcccaccagc
     1681 atccaggtcg ccggaactga ggcaattaac agcatgccaa tcagcaccat ttcctgacgg
     1741 ttcagtacat tatcatgtgt atgcgttttg cgcgcataca ggaaaaccag cagccccggt
     1801 gcatacagaa caacggataa cagcaagtgc attggaccag aggcgtacaa taaccataag
     1861 ccataaatgc aggcaccgac acctaccgct ttatgcagtg gacgggttgc gattttcagc
     1921 aagaacgcgc cgaccaggaa atagggcacc agaatcattt ctgaggcgat ggtcagcaac
     1981 gtgttgtaat cggaaccggt cagccagatg agtaccagac aaatttgcac gcagatgttg
     2041 gtcaaccaca gtgaggcgga tggcgcagct tgtgcgtcct ggcgcgcaaa aatgcgtggg
     2101 aatgctttat gagtggcagc caggaacgga acttctgccg ccatgatggt ccagcttaag
     2161 tacgcgccgc aaacggaaac gatcaaaccg gcagcgatga tgatttcgcc ccatggtccc
     2221 atcatttcca ccatcagacc ggccattgac gggttacgaa tttcagccag ttcaggacgt
     2281 gccaccacac ccagcgaaag cagcgttacc agcaagtaaa cgcccagagc ggagagaacc
     2341 gccagcagtg tcgctttgcc aacatcacgt ttattacgcg cacgcgcaga aacaaccacg
     2401 gcaccttcca caccaatgaa aacccacagg gtgatcagca tggtgttttt cacctgttcc
     2461 caaacaggta cgccaagtgc aagtccggtg aagtcgagct tgaaggtatc cagtttgaac
     2521 atcatcatcg ccagcacaac aaacagaccc agcggcaaca attttgccag tgtcgccacc
     2581 aggttaatgc tggcagcggt ttgcaccccg cgcaggatca aaaagtgaac aatccacaat
     2641 aaagccgatg caccgactat cgattgccag gtattaccat cgccaaacag gcgcaattcc
     2701 ggcgtgtccg taaaaaagct taacgcggaa aaaacgatca ccagatagga gacgttggcg
     2761 atgaccgcgc acagccagta tccccatgcg gaacaaaagc cgattagctc gccaaaccct
     2821 tcgcgggcat aggtaaagat accgccatca agttcgggac gaatgcgcgt gaggatcagc
     2881 atggcaaagg ccagcaataa aatgccagcg ccagtaatac cccagccgat gagcagtgct
     2941 gccgggctgg caactgccgc catattttgc ggcagactga aaacacccgc gcccagcatt
     3001 gagcttaata ccagcgcggt gagtgcgctc agtcccagtt tcttttccat cgatatccag
     3061 tgattaaaac gtgataagaa gaataaatat tagggcaaaa cgcataaaaa tggagtttgc
     3121 cactgaagcg cgggatttta cgaaaagatg tgtctgcatg caatggtcgc ggggaaattt
     3181 gtgactaaaa atgcgaaagg cggcatcaag ccgccttata ggagtaactg aatagttatt
     3241 tatacagatc tgcgctgacg gtcaggttat taccacgttt ctgccactgg cgggtgatgt
     3301 ggtaatactt agcacctttc ttcgcggcac gtttcgcaac ctgataggag acttcggtca
     3361 tgttgccgta gttgccagag aatttgatgc tgtcgaacgg gaccatcatc gctgctgtcg
     3421 ctttgttcag ttcttcgact ttagtgccat ccgggagcgt gacggtgtaa cgcccgcctt
     3481 ttgatgactg ggtttcaaag aagcgaccga cttcagaact tggtgatgcg gtagtcgcaa
     3541 cacccgggat ctcaactttc ttcgcggctt cgccaccggc agccagagct gcacgtcctg
     3601 cttcggaatc tgccgggatc acatccgggc tctggacgat acgtttctta gcatcttttt
     3661 tatagatgaa tgcagtaata cgctggttgc cgccctggtt ggcatcgatt tgacgaacga
     3721 tgtagaaaga gtaggcacct ttcgcttttg ccgctttggt gatggcatca ttgacttctg
     3781 gctggctacg atagaagccc tggacggtga ccgtgtcaaa cggttcaatc agaacagcct
     3841 gatctttcgg cagttcgaca acaccgttaa ttacgcgatt acttgtttct tctgcttttt
     3901 cagcatcggc tttatagagg tcagcgacca cacgccagtt accgctgtta ccaaaatcag
     3961 aagtgtcgac aacataaaaa gaggcggcac cttctttatc tgcgcgacga gaaacggctt
     4021 tcaccgcttc gccaatagca ttaaaacgac cggtaaccac tacacggtca aaaggtttaa
     4081 ccgctgccgc ttgctccggt gtcagttctg ttgctgcgtt aacggagaat gccgtagcag
     4141 aaagcagtgc cgacgccagg agggtgttct taagcttcat aaaaataatc cttcgccttg
     4201 cgcaaaccag gtactggtat tgttattaac gagaaacgtg gctgattatt gcatttaaac
     4261 ggtgtaactg tctgcgtcat ttttcatatc acattcctta aaccaatttt aatcctgctc
     4321 aaatgaccgt ctatgcttaa aaaacagccg tatcagcatc attactactg aagcaactga
     4381 attgtataag ttaatttaat gttaagtagt gattcgtgcc ggggcgatgt ctcgttttac
     4441 ccgaccgtcg aagacaatta tcagtcttta tccggcgttc taaggtgttt atcccactat
     4501 cacggctgaa tcgttaatat tttgcgagtt cacgccgaaa tactgatttt tggcgctaga
     4561 tcacaggcat aattttcagt acgttatagg gcgtttgtta ctaatttatt ttaacggagt
     4621 aacatttagc tcgtacatga gcggcttgtg tggctcctga cacaggcaaa ccatcatcaa
     4681 taaaaccgat ggaagggaat atcatgcgaa ttggcatacc aagagaacgg ttaaccaatg
     4741 aaacccgtgt tgcagcaacg ccaaaaacag tggaacagct gctgaaactg ggttttaccg
     4801 tcgcggtaga gagcggcgcg ggtcaactgg caagttttga cgataaagcg tttgtgcaag
     4861 cgggcgctga aattgtagaa gggaatagcg tctggcagtc agagatcatt ctgaaggtca
     4921 atgcgccgtt agatgatgaa attgcgttac tgaatcccgg gacaacgctg gtgagtttta
     4981 tctggcctgc gcagaatccg gaattaatgc aaaaacttgc ggaacgtaac gtgaccgtga
     5041 tggcgatgga ctctgtgccg cgaatctcac gcgcacaatc gctggacgca ctaagctcga
     5101 tggcgaatat cgccggttat cgcgccattg ttgaagcggc acatgaattt gggcgcttct
     5161 ttaccgggca aatcactgcg gccgggaaag tgccaccggc aaaagtgatg gtgattggtg
     5221 ccggtgttgc aggtttggcc gccattggcg cagcaaacag tctcggcgcg attgtgcgtg
     5281 cattcgacac ccgcccggaa gtgaaagaac aagttcaaag tatgggcgcg gaattcctcg
     5341 agctggattt taacgaggaa gcgggcagcg gcgatggcta tgccaaagtg atgtcggacg
     5401 cgttcatcaa agcggaaatg gaactctttg ccgcccaggc aaaagaggtc gatatcattg
     5461 tcaccaccgc gcttattcca ggcaaaccag cgccgaagct aattacccgt gaaatggttg
     5521 actccatgaa ggcgggcagt gtgattgtcg acctggcagc ccaaaacggt ggcaactgtg
     5581 aatacatcgt gccgggtgaa atcttcagta cggaaaatgg tgtcaaagtg attggttata
     5641 ccgatcttcc gggccgtctg ccgacgcaat cctcacagct ttacggtact aacctcgtta
     5701 atctgctgaa actgttgtgc aaagagaaag acggcaatat cactgttgat tttgatgatg
     5761 tggtgattcg cggcgtgacc gtgatccgtg cgggcgaaat tacctggccg gcaccgccga
     5821 ttcaggtatc agctcagccg caggcggcac aaaaagcggc accggaagtg aaaactgagg
     5881 aaaaatgtgc ctgctcaccg tggcgtaaat acgcgttgat ggcgctggca atcatcctgt
     5941 tcggttggct gtcaagcgtt gcgccgaaag agtttcttgg gcacttcacc gttttcgcgc
     6001 tggcctgcgt tgtcggttat tacgtggtgt ggaatgtatc gcacgcgctg catacaccgt
     6061 tgatgtcggt taccaacgcg atttcaggga ttattgttgt cggagcactg ttgcagattg
     6121 gccagggagg ctgggttagc ttccttagtt ttatcgcggt gcttatagcc agcattaata
     6181 ttttcggtgg cttcaccgtg actcagcgca tgctgaaaat gttccgcaaa aattaagggg
     6241 taacatatgt ctggaggatt agttacagct gcatacattg ttgccgcgat cctgtttatc
     6301 ttcagtctgg ccggtctttc gaaacatgaa acgtctcgcc agggtaacaa cttcggtatc
     6361 gccgggatgg cgattgcgtt aatcgcaacc atttttggac cggatacggg taatgttggc
     6421 tggatcttgc tggcgatggt cattggtggg gcaattggta tccggctggc gaagaaagtt
     6481 gaaatgaccg aaatgccaga actggtggcg atcctgcaca gcttcgtggg tctggcggca
     6541 gtgctggttg gctttaacag ttatctgcat catgacgcgg gaatggcacc gattctggtc
     6601 aatattcacc tgacggaagt gttcctcggt atcttcatcg gggcggtaac gttcacgggc
     6661 tcggtggtgg cgttcggcaa actgtgtggc aagatttcgt ctaaaccgtt gatgctgcca
     6721 aaccgtcaca aaatgaacct ggcggctctg gtcgtttcct tcttgctgct gattgtattt
     6781 gttcgcacgg acagcgtcgg cctgcaagtg ctggcattgc tgataatgac cgccattgcg
     6841 ctggtattcg gctggcattt agtcgcctcc atcggtggtg cagatatgcc ggtggtggtg
     6901 tcgatgctga actcgtactc cggctgggcg gctgcggctg cgggctttat gctcagcaac
     6961 gacctgctga ttgtgaccgg tgcgctggtc ggttcttcgg gggctatcct ttcttacatt
     7021 atgtgtaagg cgatgaaccg ttcctttatc agcgttattg cgggtggttt cggcaccgac
     7081 ggctcttcta ctggcgatga tcaggaagtg ggtgagcacc gcgaaatcac cgcagaagag
     7141 acagcggaac tgctgaaaaa ctcccattca gtgatcatta ctccggggta cggcatggca
     7201 gtcgcgcagg cgcaatatcc tgtcgctgaa attaccgaga aactccgcgc tcgtagtatt
     7261 aatgtgcgtt tcggtatcca cccggttgcg gggcgtttgc ctggacatat gaacgtattg
     7321 ctggctgaag caaaagtacc gtatgacatc gtgctggaaa tggacgagat caacgatgac
     7381 tttgctgata ccgataccgt actggtgatt ggtgctaacg atacggttaa cccggcggcg
     7441 caggatgatc cgaagagtcc gattgctggt atgcctgtgc tggaagtgtg gaaagcgcag
     7501 aacgtgattg tctttaaacg ttcgatgaac actggctatg ctggtgtgca aaacccgctg
     7561 ttcttcaagg aaaacaccca catgctgttt ggtgacgcca aagccagcgt ggatgcaatc
     7621 ctgaaagctc tgtaaccctg acggcctctg cggaggccgt cactctttat tgagatcgct
     7681 taacagaacg gcgatgcttt gacctcccgc tgtttgttca agcgcaattt tgacaataat
     7741 tgtcaacggc acggaaagca gcatacccac cggtcctaac aaccatcccc aaaaaatcaa
     7801 cgacaaaaat accaccaatg tcgaaagccc cagcccacgt cccatgatgc gcggctccag
     7861 aatattgccg aagaccagat taatcagcag atatcccgcc agcaccagca acgcttcgta
     7921 gaagccatta aacaccagta cctgagcgat aggggggatt gccgcgagaa ctgaaccaat
     7981 attagggatg taattaagcg caaaggccag caatccccag acaaaagcga agtgaacatc
     8041 gagtgcggcg agcatcgccc aggcgaccaa gccggtgatg atgctgatgg ctgttttcag
     8101 caccagataa tgagaaacac tgtcaatcgc acgttgaatc gccgccatcc cttcaaccgg
     8161 acgcgccatc atttgctgaa attttccggg caattgtggc acttcgagca gcataaacag
     8221 caccgtcagc agcaataaaa atattgatga catggcatta gataactgcg tcaataagtt
     8281 ggtgagcaac gtcatcgccg cgttcggatc aatgtaatgc gccagttggt caactgagac
     8341 gtcaatccct acgcgttgca acaacggctc aagagcttgc agcggcgtca taatagagtt
     8401 gcgatattgc ggtaacgtcc gcgtcaactc gttgagcgcg gaacccagat aagctaatag
     8461 caacaccatc gccatcacga tgattgtcat caaaatcgac actgccagta cacgcggcac
     8521 acgccagcgg accatatgtt gcaccagcgg gttaagaata acagcaataa ataatgcgag
     8581 aataaacggc acgatgatct cggcggcaaa acggataccg cagagaataa tgaccagcat
     8641 tcccaacata atgacgattt ttaggccatt gagcgtgatg atcggctttg ccatgcttgt
     8701 ccccgttttt cttcctttgc taataataat gtctttttcg ctccgtcatc ataacgaaaa
     8761 tcaaaccgct caggtaaaga agtgaaaata ttttgagtta attcttaagc tatgatacaa
     8821 atcaggcgtg ttcaactacc gaggacaatt atcatctgcg atgacgagaa gcaacactgc
     8881 ggataattgt aatattatgg acaatttgtt caggacgtta ttttctacat ttacccatct
     8941 tcgtacctca tccactatct tgctcgtagg tgagcagcac tggcgcaacg cgctttagtc
     9001 ctttcttctg cctaagcttg ccctatgcgc agggcttgac cactttactt tggtttcgct
     9061 gaattaagcg aaaattaaaa tagttctctt gcaggagaag gacaatgtat atttattgga
     9121 ttttattagg tctggctatt gctacagaaa ttaccggtac gctgtcaatg aaatgggcga
     9181 gcgtcagtga gggaaatggc ggctttattt taatgctggt gatgatttct ctgtcgtata
     9241 tatttctctc tttcgccgtt aaaaaaatcg ccttaggcgt agcttatgcg ctgtgggaag
     9301 gtatcggtat tttatttatt accttgttta gcgttttgtt attcgacgaa agtttatcgc
     9361 tgatgaaaat tgccgggtta accaccctgg tcgccgggat agtgttgatc aaatcaggta
     9421 cccgtaaagc gcgtaaacct gaactggagg tgaaccatgg cgcagtttga atgggttcac
     9481 gccgcctggc tggcattggc aatcgtgctg gaaatcgttg ctaacgtctt tttgaaattt
     9541 tctgacggct ttcgtcgcaa aatatttggc ttgctctccc tggcggcggt gctggcggcc
     9601 tttagtgcgc tttctcaggc cgttaaaggg atcgacttgt ctgtcgctta tgcattgtgg
     9661 ggcgggtttg gtattgccgc cacgttagcc gcaggttgga tcttgtttgg tcaacggtta
     9721 aatcgtaaag gctggattgg cctggtcttg ctgttggctg gaatgatcat ggtgaaactt
     9781 gcctgatgaa gacgctgccc gcgctgtcgg gcagcgtttg aacattattt ttgcgacagt
     9841 tgatccagct tgtcgcgaaa accggtaaca gaaatggccc ggttatcggc gcgccagcga
     9901 tctttcgcgg caggagccga actttgcacc ccaattaatt gccagccgtc atcggtatgc
     9961 aacatcagag gcgaaccgct gtcacccggc aaggtatcgc actgatgtga catcaccgac
    10021 gtttgcgccc agccagtcac ttcacagttt tgatgactgt ataacgtatc gagatgatct
    10081 tcagggtatc ccgcctgagt cactttacga ccagccgatt ttaatgcggc agtaagcgcg
    10141 gctttatctc cctcaaataa cggcaacggt gtaatgccag aagggggatt acgtagcaca
    10201 atcaatccga agtcccacgg tgcagctgcg ggaggaacaa tccaaccatc cccatctgct
    10261 tttaaccgct ttcccagtgt cggatcaacg cggccttcta tgtcgtggat ctcatagcgc
    10321 caaagacctt tatttgacac aaaacgcagc gctaccgcct tatccgcttt acccttaggc
    10381 ggcgtcaata aacagtgtcc tgccgttaat gccagattgg gtgcaatcag cgtcgccgta
    10441 cataaattgc cgctggccgt ttccagttgc ccaaccgcat cccacggtga ttgggtcgtg
    10501 tcattcactg gcacacgatc atcatgacca aaaaacaggg tgctgacctc atcgtttgcc
    10561 gatttggcaa cgtctggttt atctgcaaac acaaaagcag acgtcaaact aattgcaccc
    10621 aacactacag caatggttgt acgcatatca cactctggtg ggtaattatg attattaaaa
    10681 gcaaaccctc ataaatacta tagacgggac gggatgaaag tgggagtaaa atcagataac
    10741 tacagtcagg aaagataaaa acgggcggca atcagtgcgc ataaaataag gatgataaga
    10801 atgaactcaa agcgataacg gcccaccata ctaccctccg ctaaaggcgg tgctaaacgt
    10861 tacccgctta gcaccgtaat tttcaggcgc gagggggcgc gccagcatta ctgttgaaaa
    10921 cttacgctgc gggttgtgca gcaggttttg ccggttgctg atggccgtgt ttcccggcgt
    10981 gtttcttcgc tgcctgcgct ttttgttcag gggctttctg ttcagctttc gtatttttat
    11041 gatgcttttt agccgcctgc gctttctggg caggtgctgc tttatgctgt tttttatgat
    11101 gtgtagtttt cgctggcgct gctttggtgg tcgtcgcagt cggagcaggt gtggtcgcag
    11161 tctctgcagc aaaggcggca gaagacagac ccatagcagc ggcaacaacc agagctaata
    11221 cttttttcat tgtcataccc tcaatttgtt ttttcattta accccactgc ggggccgttg
    11281 aaataactat atccctggga atggcagact tccgtgagtg gttggtttca gcgtgtaacg
    11341 atatgtacaa acgctgaata aattacagcg ttgataataa cgtattgtga cttaaggaaa
    11401 atttagctac caataatagt agtcttgatc gggttaacta ttatactact tacaaagc
//
LOCUS       DAJTNM010000121        11438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTNM010000121 DAJTNM010000000
VERSION     DAJTNM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.121"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="OO172_003344"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="OO172_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5513414.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTVLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="OO172_003345"
     CDS             complement(2005..2928)
                     /locus_tag="OO172_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5513415.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGHAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINTIRKSMPF"
     gene            3145..4488
                     /locus_tag="OO172_003346"
                     /pseudo
     CDS             3145..4488
                     /locus_tag="OO172_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="OO172_003347"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="OO172_003347"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5513416.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTLQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="OO172_003348"
     CDS             6508..6732
                     /locus_tag="OO172_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5513417.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="OO172_003349"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="OO172_003349"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5513418.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /locus_tag="OO172_003350"
     CDS             complement(7326..8306)
                     /locus_tag="OO172_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR5513419.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="OO172_003351"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="OO172_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5513420.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="OO172_003352"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="OO172_003352"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5513421.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10983
                     /locus_tag="OO172_003353"
     CDS             10315..10983
                     /locus_tag="OO172_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5513422.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11001..11438)
                     /gene="tnpA"
                     /locus_tag="OO172_003354"
                     /pseudo
     CDS             complement(11001..11438)
                     /gene="tnpA"
                     /locus_tag="OO172_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2952 a         2669 c         2871 g         2946 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg tgttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cctcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggccgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatacag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag tgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agcagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagact
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgt gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgtaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg ctgttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcactag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaagaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgctgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcagg
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact cgcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cccgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga ggaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgcgcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatattct ggttttttaa acaattactc tgatttaaaa
    10441 gaaacaacct cagcaacagg taaacctgtt ttacgttggg tagacccgag ttttgatcaa
    10501 agcaaatatg acagcatcgt ctggaaccca atcacttatt atccggtacc aaaaccttct
    10561 acccaggtag ggcagaaagt tctggataaa attttgaact ataccaacac cgaaatgaaa
    10621 gaagcgatag cgcagcgtaa accagtggtt accaccgctg ggccgcgtag tctgattttc
    10681 cgtggggcca ttaccggtgt agataccagc aaagaagggc tgcaattcta tgaagtggtt
    10741 cctgttgcat tagtggttgc ggggacgcaa atggctacag gccaccgtac catggatact
    10801 cgcctctatt ttgaaggtga gctgattgat gcagcgacta ataaaccggt tatcaaagtc
    10861 gttcgtcagg gcgaaggtaa agacctgaat aacgaaagta cgccaatggc tttcgaaaat
    10921 attaaacaag ttattgatga catggcgacc gatgccacca tgtttgacgt taacaaaaag
    10981 tagttccaga cgcgccatcg ttagatggcg ctttttatcc ggtgcgccgt aaaaccccat
    11041 ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgtttg ctgttgttca
    11101 atgtattggc ggatgacgga tattggcgca cctccgcagc tactggcaaa atagccagga
    11161 ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt
    11221 aacctacccg atacgccttt gaggctgttt accagactgg atatcgccag tttgggaggt
    11281 tagttaatta acaaatggac gtgatctggt tcgccatcca tttcaaccag ttcagcttca
    11341 aaataagcac atacatttga aaagtaagtg cgtagttttt ctgtcgcgtc atggtcaaaa
    11401 atctggcgtc ggtatctggt gacaaagacc aggtgaac
//
LOCUS       DAJTNM010000122        11438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTNM010000122 DAJTNM010000000
VERSION     DAJTNM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.122"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            9..461
                     /locus_tag="OO172_003355"
     CDS             9..461
                     /locus_tag="OO172_003355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5513423.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYVFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            707..1751
                     /locus_tag="OO172_003356"
                     /pseudo
     CDS             707..1751
                     /locus_tag="OO172_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1813..2427
                     /locus_tag="OO172_003357"
                     /pseudo
     CDS             1813..2427
                     /locus_tag="OO172_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="OO172_003358"
     CDS             complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="OO172_003358"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5513424.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4203..4661
                     /gene="gpt"
                     /locus_tag="OO172_003359"
     CDS             4203..4661
                     /gene="gpt"
                     /locus_tag="OO172_003359"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5513425.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4753..5997
                     /gene="frsA"
                     /locus_tag="OO172_003360"
     CDS             4753..5997
                     /gene="frsA"
                     /locus_tag="OO172_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5513426.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6055..6456
                     /gene="crl"
                     /locus_tag="OO172_003361"
     CDS             6055..6456
                     /gene="crl"
                     /locus_tag="OO172_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5513427.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="OO172_003362"
     CDS             complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="OO172_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5513428.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7838..8941
                     /gene="proB"
                     /locus_tag="OO172_003363"
     CDS             7838..8941
                     /gene="proB"
                     /locus_tag="OO172_003363"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5513429.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8953..10206
                     /gene="proA"
                     /locus_tag="OO172_003364"
     CDS             8953..10206
                     /gene="proA"
                     /locus_tag="OO172_003364"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5513430.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10321..10396
                     /locus_tag="OO172_003365"
     tRNA            10321..10396
                     /locus_tag="OO172_003365"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10354..10356,aa:Thr,seq:cgt)
     gene            complement(10411..>11438)
                     /locus_tag="OO172_003366"
     CDS             complement(10411..>11438)
                     /locus_tag="OO172_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5513431.1"
                     /translation="WRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLH
                     FREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKT
                     RHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVV
                     MFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQG
                     LHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWAS
                     WLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEEN
                     VVYLNAR"
BASE COUNT         2744 a         2838 c         2946 g         2910 t
ORIGIN      
        1 tttatattat gaataacata caaataagag attatcagcc tggcgatttt cagcaactat
       61 gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca ccacaacaaa
      121 ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag
      181 tgcgggttgc agtcattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata
      241 tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa
      301 aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct
      361 tttttgaacg ttatgttttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt
      421 ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa
      481 taacgcccgc ctagtatgtg cgcgttttct tatttcacaa actgcaagga ggtaaatcat
      541 gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg
      601 ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc
      661 agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat
      721 tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt
      781 agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat
      841 gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg
      901 tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga
      961 tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga
     1021 cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg
     1081 gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga
     1141 tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact
     1201 gctggttcat agcggctcga gggagctagg gcagtctatt ttacagcggc atattgcctc
     1261 gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa
     1321 tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct
     1381 ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca
     1441 tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga
     1501 ttactccagg ctggttcagg cccgtcagga gtgaggaaac attgcattcg ctggcgcatg
     1561 gggctgggcg taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag
     1621 cgacgcaact ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca
     1681 tcttcgaaga agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtctgggca
     1741 ggccggatta attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg
     1801 agggaaaaaa ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt
     1861 ctcgcagtga aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg
     1921 gtaacggtgc tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt
     1981 tctctggatg gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtgg
     2041 atttgcccga gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg
     2101 cgttttaccg ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg
     2161 tcggggccgg gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg
     2221 gcatccggta ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg
     2281 gcgcgattgc tgattgcctg gaagctggat cagcagcaat aggaaaatag cgcggtgctg
     2341 aaatcacagc ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt
     2401 acagggatgg cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg
     2461 ccatccggca gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc
     2521 agtgtccagt aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg
     2581 gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca
     2641 cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag
     2701 gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg
     2761 cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg
     2821 tagtctttac cgctgtcgat cagtgaacgg atcaggcagt gaatttctac gttattgtca
     2881 gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa
     2941 ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc
     3001 gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct
     3061 ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg
     3121 tcgactttat cagctgtgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc
     3181 agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg
     3241 aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga
     3301 ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca
     3361 cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca
     3421 ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag
     3481 ccgaacgcac cgtccatacc ggcttcttcg gttatggtca gcagcacttc cagcgggccg
     3541 tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc
     3601 gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga
     3661 tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc
     3721 tgtaagacga ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata
     3781 ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat
     3841 tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata
     3901 tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat
     3961 cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca
     4021 ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     4081 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatatc tataatctcg
     4141 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     4201 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     4261 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     4321 tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     4381 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     4441 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga
     4501 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     4561 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg
     4621 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat
     4681 tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg
     4741 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     4801 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
     4861 tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     4921 atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     4981 gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac
     5041 tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa
     5101 gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc
     5161 tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc
     5221 tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc
     5281 ggcggtgcgc ccatcaccgg ctttttgcat atgccgcaag gcgatggccc gttcccgaca
     5341 gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt
     5401 tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct
     5461 tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg
     5521 cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct
     5581 aacgttgccg tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt
     5641 ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg
     5701 tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc
     5761 gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg
     5821 ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta
     5881 atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg
     5941 aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa
     6001 tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg
     6061 ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat
     6121 attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc
     6181 aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg
     6241 caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg
     6301 aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag
     6361 tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcggat
     6421 gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca
     6481 tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta
     6541 atattcagct tattatcgct atccagtttg ttgattttat aatcaacaaa cgctgacata
     6601 tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg
     6661 atgccctcaa tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag
     6721 tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt
     6781 attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg
     6841 tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc
     6901 tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca
     6961 aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt
     7021 tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg
     7081 gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc
     7141 gaggaatcgc caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg
     7201 cccaggttac gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg
     7261 agacgcgttt tttgctgtgc agtatcactc tccgctttat ttccggcaaa ctccgcttcc
     7321 caccgaccat aaccagtcag ttgatcgtta atttgagttt cacctttaaa accaaaacgg
     7381 atataactct ggtcgccatc tttactggcg ttatcactca tataatgcat ggctttaact
     7441 ttgccataga catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat
     7501 gcagatgcca caatgcccat caccactaat gccagagtgc tctttttcat tttcattcct
     7561 gattttaatt aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt
     7621 aacctgagat tgttaaagat atattacaga ttaatgatat tctaaaaatg tggtaattta
     7681 ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc gcgcaacaaa
     7741 acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt
     7801 tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc agacgctggt
     7861 ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat
     7921 cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt
     7981 gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac tgccagcgac
     8041 tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga
     8101 acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat
     8161 ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgatcacaa
     8221 tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga
     8281 taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct
     8341 gaccgatcaa aaaggtttgt ataccgctga tccgcgcagc aatccgcagg cagaactgat
     8401 taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg
     8461 cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt gccgtgcggg
     8521 tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg
     8581 catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg
     8641 gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat
     8701 tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc
     8761 gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc acggtgtcag
     8821 tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc
     8881 aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta
     8941 aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc gtataaatta
     9001 gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa
     9061 gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat
     9121 ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt
     9181 gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc
     9241 ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc
     9301 gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc
     9361 ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc ggcaacggtg
     9421 gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt gcaggcgatt
     9481 gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg
     9541 ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg
     9601 gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagcgc agagatcgct
     9661 gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg taatacggtt
     9721 gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa
     9781 atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca gttgcaggca
     9841 ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt tctgtcatta
     9901 gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac
     9961 ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt
    10021 aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc
    10081 cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc gcgtggccca
    10141 atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt
    10201 gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc attgacgcat
    10261 cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg
    10321 gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt aggttcgact
    10381 cctattatcg gcaccattta aatcaataag ttacctcgca tttaagtaaa ccacgttctc
    10441 ctcttgtgcc gtatttgtgc cattgcgact tataatcgca tcgattttgc ttgcgtgctc
    10501 ggtgagatgc ccggctgaaa ggtgggcgta tctttgaacc atttcgagag tttcccatcc
    10561 tcccatctct ttaagtgcaa gaagagagac accggactga accagccagc ttgcccaggt
    10621 atgcctcaga tcatggaagc ggaagttgct aatgcctgcc cgctttaacg ctcccttcca
    10681 tgccttgttg ctgtcggttc tcatcttcct taccgctgct gtttttgttc cgtcgcttcg
    10741 gtaggcaggt ttggtgtgga caaataccca tctcttatgg agcccctgct gttttcttaa
    10801 tatctggcat gcggtttcgt taagaggaac tccgatcgca ttgccagctt ttgtttcatc
    10861 agggtgcatc catgccattt tcttatccag atcgacctgt gaccactcaa ggtctgtaac
    10921 gttggaacgg cgaaggcctg tcgtgattgc aaacatgacc acagggaaga agtgaggagc
    10981 aatttctgca aacaggcgct tcgattcctc ctctgtaagc catctgatgc gtccattctt
    11041 aacgcgtggt gttgatattt tgggtgccct gtcaagccat ccccattcaa cagccatatt
    11101 gagaatggcg cgaagtattg ccagatgcct cgtcttcgtt cctttgcttg caagctttgg
    11161 tttatactcc ggcaccggat tgccaagtcg caaacacctg tcccggctca tctcccagtt
    11221 caggcgatgg cggcggtttt ccatcccgtc taccgcctcc attatttttt ctgttgttat
    11281 gtcagagaga atggcttctc tgaagtgcaa catccagaac gatataatgc tcttgtcatc
    11341 atcaatggac ttcttatccg atttctcacg cagccaccgt atgcaggctt ccttgaatag
    11401 ctttttcggt gattccccga gattttttac tctccacg
//
LOCUS       DAJTNM010000123        11234 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTNM010000123 DAJTNM010000000
VERSION     DAJTNM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.123"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(77..1741)
                     /gene="glnS"
                     /locus_tag="OO172_003367"
     CDS             complement(77..1741)
                     /gene="glnS"
                     /locus_tag="OO172_003367"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5513432.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1944..3890)
                     /gene="nagE"
                     /locus_tag="OO172_003368"
     CDS             complement(1944..3890)
                     /gene="nagE"
                     /locus_tag="OO172_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5513433.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4223..5023
                     /gene="nagB"
                     /locus_tag="OO172_003369"
     CDS             4223..5023
                     /gene="nagB"
                     /locus_tag="OO172_003369"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5513434.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5083..6231
                     /gene="nagA"
                     /locus_tag="OO172_003370"
     CDS             5083..6231
                     /gene="nagA"
                     /locus_tag="OO172_003370"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5513435.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6240..7460
                     /gene="nagC"
                     /locus_tag="OO172_003371"
                     /gene_synonym="nagR"
     CDS             6240..7460
                     /gene="nagC"
                     /locus_tag="OO172_003371"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5513436.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7508..8250
                     /gene="nagD"
                     /locus_tag="OO172_003372"
                     /pseudo
     CDS             7508..8250
                     /gene="nagD"
                     /locus_tag="OO172_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            8647..10311
                     /gene="asnB"
                     /locus_tag="OO172_003373"
     CDS             8647..10311
                     /gene="asnB"
                     /locus_tag="OO172_003373"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5513437.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10691..10767
                     /locus_tag="OO172_003374"
     tRNA            10691..10767
                     /locus_tag="OO172_003374"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10725..10727,aa:Met,seq:cat)
     gene            10776..10860
                     /locus_tag="OO172_003375"
     tRNA            10776..10860
                     /locus_tag="OO172_003375"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10810..10812,aa:Leu,seq:tag)
     gene            10884..10958
                     /locus_tag="OO172_003376"
     tRNA            10884..10958
                     /locus_tag="OO172_003376"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10916..10918,aa:Gln,seq:ttg)
     gene            10993..11067
                     /locus_tag="OO172_003377"
     tRNA            10993..11067
                     /locus_tag="OO172_003377"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11025..11027,aa:Gln,seq:ttg)
     gene            11083..11159
                     /locus_tag="OO172_003378"
     tRNA            11083..11159
                     /locus_tag="OO172_003378"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11117..11119,aa:Met,seq:cat)
BASE COUNT         2791 a         2902 c         2838 g         2703 t
ORIGIN      
        1 ttagcgtttg aaatttattg tctatttatt gccggatgcg acgtaaatgc ctcacccggc
       61 ttggtagaac ttaaaattac tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc
      121 ggttaaatac cggtttttcg gccgtggaat ggcggctgtc gaggcagaag taaccttcac
      181 gctcaaactg gaatgcttta cccgctaccg catctttcag cgacggttca gcaaagccct
      241 gtttgatcac cagcgattcc gggttaatca ccgacaggaa atcatccgca gcacctgggt
      301 taggcacgct gaacagacga tcatacaaac ggatttcaac cggcagcgca tgtgccgcgc
      361 tcacccagtg aataacacct ttgactttac gaccatctgc cggatctttg cttaaggtgt
      421 cggcgtcata agtacagaag atggtggtga tattaccttc ggcatctttc tccacgcgtt
      481 cagccttaat gacgtaagca ttacgcagac gcacttcttt acccagcacc agacgtttgt
      541 actgcttgtt agcttcttcg cggaaatcgg cgcgatcgat ccaaatttca ccgctaaacg
      601 gcacctgacg gctccccatt tccggtttgt tcggatggtt cggcatggtg accatttcgc
      661 cttcgccctg atagttttcg ataaccagtt tcaccggatc gataactgcc attgcgcgcg
      721 gcgcattttc gttgagatct tcacggatgc aggattccag cgacgccatc tcaatggtgt
      781 tgtcctgctt ggtcacgccg atgcgtttgc agaactcacg aatagaagcc gcagtgtaac
      841 cacgacgacg cagaccggaa atggtcggca tacgcgggtc atcccagcct tcaacgtgct
      901 tgtcggtcac cagcaggttc agcttacgct tggacatcac agtgtattcc agattcaggc
      961 gcgagaattc gtactggcgc ggatgaacag gaatggtgat gttatccagt acccagtcgt
     1021 acagacgacg gttgtcctgg aactcaagcg tacacagaga gtgcgtaata ccttccagcg
     1081 catcgctgat gcaatgggtg aagtcgtaca tcgggtagat gcaccacttg ttgccagtct
     1141 ggtggtgttc agcaaattta atgcggtaca gcaccggatc gcgcattacg ataaatggcg
     1201 acgccatgtc gattttcgca cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca
     1261 ttttttcgaa cagcgccaag ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac
     1321 ccggctgcgt cagggtaccg cggtattcgc ggatctgttc cggtgtcagt tcatcaacgt
     1381 acgccaggcc tttattgatc agttcgatcg cataggcgtg gagctgatca aaataatcgg
     1441 aggagtaacg gacgttacca gaccagtgaa aacctaacca ctctacgtcg tttttgatcg
     1501 aatcgacata ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact
     1561 ggcctttata gtcctgggcg atcccgaagt tcaggcagat agatttcgca tggccaatat
     1621 gcagatagcc attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg
     1681 ccagatcttc atcgatgatc tgacggataa agttagtcgg gcgggcttct gcctcactca
     1741 tcgtggattc ctcaaagcgt aaacaacgta taacggcgta tgatcttata agcgggacag
     1801 gctgacaact gttagttttt taaaattgat aatctaacaa ggattatggg gatgattgat
     1861 gaaaaaaaaa gcggcagaga agatctccac cgcttgtcat tgttggatgc gacgctgccg
     1921 tgtcgcatca ggcataaagc agattacttt ttgatttcat acagcggtgt ttgacccgcc
     1981 acaacatggc cctgagcttt aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc
     2041 accgggctaa tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat ttccagaatc
     2101 ggttgccctg cgcttacctg cgcaccctct tccaccagac gtttaaagcc tttaccttcc
     2161 agcgctacgg tgtcgatacc catatggacg acgatctccg cgcctttttc ggtttccagg
     2221 cagaacgcgt ggttggtgtt gaagattttc acgattgtcc ctgcggctgg tgatacgacg
     2281 attttatctg tcggtttcac cgccacaccg tcacccaccg ctttgctggc gaatgcttcg
     2341 tcaggaacct gatccagtgc cacgacatca ccggtaatcg gcgataccag ctccgcgata
     2401 gataccgcgt ttggtacagc ctgcggtttt gctacaggcg cggcagttgc cggagttgct
     2461 tcagctgacg cagcggctac cggaccacgg gcaacgactt tcttcatcgc atcgccgatg
     2521 gattctgctt tcgcgccaac aatcacctga atagtctgtt tgttcagttt cactacacca
     2581 gaagcaccca gacgtttaca catcgtatcg ttgacgcggg cagagtcagc cacggtcagg
     2641 cgcagacggg taatacaggc atcaatcgct ttcaggttgt cagtaccgcc aaccgcagca
     2701 atatagttgg ttgccagttg agtcagacct tcttcagtat tgctgttggc ttcttcagta
     2761 acgatctcgt cttctttatc ttcacgaccc ggcgttttca ggttgaacat gcggataacc
     2821 aaactgaaca ccacgaagta gatagcgaag aagataacgc ccatcaccag cagcatccag
     2881 acgttctggc tggcggccgg caggttatac atcaacgcgt agtcgatagc acccgcagag
     2941 aaagagaagc cggcatggat acccagcagc gttgccacaa acaggctgat accggtcagc
     3001 agtgcgtgca ggaggtacag cagcggcgca aggaacatga acaggaattc cagcggctca
     3061 gtcacaccgg tcaggaacgc agtaacagca acagaaagca gcatcccgcc aaccatcgga
     3121 cgacgctctt tcggtgctgc gaagtacatc gccagcgccg cacccggcag accgaacatc
     3181 atgatcggga agaagccgga catgaacatc cccgcggtgc cgtcaccagc gtagaaacgg
     3241 ttgatgtcgc cgtggaaaac cgtacccgcc gcgttggtga attcaccaat ctggaaccag
     3301 gcgatggtgt tcagcacctg atgcagaccg gttgggatca gcagacggtt gatgaaacca
     3361 aagataccgg aacccagcgc gcccgcagaa acgatccact cgccgcctgc atggatagcg
     3421 tgctgtaccg gcggccagac gtaaccaaaa atggccgcca gcaccagaca gaagaagccg
     3481 gtggcgatcg gcacaaagcg tttgccgccg aagaagctca ggaagtccgg cagtttaata
     3541 tcggaccaac ggttataggc tgcgccacca accagaccgg taatgatacc cgccagtaca
     3601 cccatgttaa tttctgggtt gatggtcacc atcgctttgg ttaacacaaa gtaacctacc
     3661 gcacccgcca gcgccgccgc acctgcgctg tctttcgacc agctggatgc cacaccgatg
     3721 gcgaagatta atgcgaggtt atcaaaaatt gcaccgcccg cctgggcaat aaacgcaacg
     3781 ttaagtaaat ctggctgacc gaatcgcagc aacagcgccg ccaccggcag caccgcgata
     3841 gggagctgta acgccctacc gagtcgctgg aaaaaaccta aaatattcat cttattcccc
     3901 ctacgagaac cctatttggc tcgtttaaag ccgtattttt attttgctgc aaattttact
     3961 gccgatgttc tgtaatcaga ttgttagatc atctgctaca gagtgtgtga aaatttaatt
     4021 cgtatcgcaa attaaacgcg tgtcttttgt gagttttgtc accaaatatc gttatcatca
     4081 ctccctttta ctggctaaac cagaaaactt attttatcat tcaaaaaatc aagtcggatt
     4141 gacgcctgtc tgcgcaaatc caggttacgc ttaaagatgt ctaatccgcc aacggcttac
     4201 attttactta ttgaggtgaa taatgagact gatccccctg actaccgctg aacaggtcgg
     4261 caaatgggct gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccaa ctgccgatcg
     4321 tccgtttgta ctgggcctgc cgactggcgg cacgccgatg accacctata aagcgttagt
     4381 cgaaatgcat aaagcaggcc aggtcagctt taagcacgtt gtcaccttca acatggacga
     4441 atatgtcggt ctgccgaaag agcatccgga aagctactac agctttatgc accgtaattt
     4501 cttcgatcac gttgatattc cagcagaaaa catcaacctt ctcaacggca atgccccgga
     4561 tatcgatgcc gagtgccgcc agtatgaaga aaaaatccgt tcttacggaa aaattcatct
     4621 gtttatgggc ggtgtaggta acgacggtca tattgcattt aacgaaccgg cgtcttctct
     4681 ggcttctcgt actcgtatca aaaccctgac tcatgacact cgcgtcgcga actctcgttt
     4741 ctttgataac gatgtcaatc aggtgccaaa atatgccctg actgtcggtg ttggtacact
     4801 gctggatgcc gaagaagtga tgattctggt gctgggtagc cagaaagcac tggcgctgca
     4861 ggccgccgtt gaaggttgcg tgaaccatat gtggaccatc agctgtctgc aactgcatcc
     4921 gaaagcgatc atggtgtgcg atgaaccttc caccatggag ctgaaagtta agactttaag
     4981 atatttcaat gaattagaag cagaaaatat caaaggtctg taattgttat ccctgccctg
     5041 gttccttgct cagggcaata ttttttaaaa tcgggggtca gaatgtatgc attaacccag
     5101 ggccggatct ttaccggcca cgaatttctt gatgaccacg cggttgttat cgctgatggc
     5161 ctgattaaaa gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca acgttcactg
     5221 aacggggcca ttctctcccc cggttttatc gatgtgcagt taaacggctg cggcggcgta
     5281 cagtttaacg acaccgctga agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc
     5341 aatgagaaat caggctgtac taactatctg ccgacgctta tcaccaccag cgatgagctg
     5401 atgaaacagg gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa tcaggcgtta
     5461 ggtctgcatc tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca taatccgaat
     5521 tttgtgcgta agcctgatgc cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt
     5581 accaaagtga ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa actggcaaat
     5641 gccgggattg tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt
     5701 ttccgcgcgg ggattacctt tgccacccat ctgtacaacg cgatgccgta tattaccggt
     5761 cgtgaaccgg gcctggcggg cgcgatcctc gacgaagctg acatttattg cggtattatc
     5821 gctgatggcc tgcatgttga ttacgccaac attcgtaacg ctaaacgcct taaaggcgac
     5881 aaactgtgtc tggttaccga cgccaccgcg ccagcaggtg ccaacattga acagttcatt
     5941 tttgcgggta aaacaatata ctaccgtaac ggactttgtg tggatgagaa cggtacgtta
     6001 agcggttcat ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc
     6061 gcactggatg aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat tgacgttgaa
     6121 aaacgtctcg gcacgctcgc cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat
     6181 tttaaaatca ccaagaccat cgttaacggt aacgaggtcg taactcaata agagaaagta
     6241 tgacaccagg cggacaagct cagataggta atgttgatct cgtaaaacag cttaacagcg
     6301 cggcggttta tcgcctgatt gaccagtacg ggccaatctc gcggattcag attgccgagc
     6361 aaagccagct tgcccccgcc agcgtaacca aaattacgcg tcagcttatc gaacgcgggc
     6421 tgatcaaaga agttgatcag caggcctcca ccgggggccg ccgcgctatc tccatcgtca
     6481 ccgaaacccg caatttccac gcaatcggcg tacggcttgg tcgtcatgac gccaccatca
     6541 ctctgtttga tctcagcagc aaagtgctgg cagaagaaca ttacccgctg ccggaacgta
     6601 cccagcagac gctggaacat gccctgctga atgccattgc tcagtttatt gatagctacc
     6661 agcgcaagct gcgcgagcta atcgccattt cggtgatcct gccagggctt gttgacccgg
     6721 acagcggcaa aattcattac atgccgcata ttcaggtaga aaactggggg ctggtagaag
     6781 cactgaaaga acgttttaaa gtgacctgtt tcgttggtca cgatatccgt agtctggcgc
     6841 tggcggagca ctacttcggt gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc
     6901 atcgcggaac cggggccggg attatctcta acgggcgcat ttttattggc cgcaacggta
     6961 acgtcggtga aattggccat attcaggtcg aaccgctggg tgaacgctgc cactgcggca
     7021 actttggctg cctggaaact atcgccgcca acgctgccat tgaacaacgg gtgttgaatc
     7081 tgttaaagca gggctaccag agccgtgtgc cactggacga ctgcaccatc aaaactatct
     7141 gcaaagccgc gaacaaaggc gatagcctgg cctcggaagt gattgagtat gtcggtcgtc
     7201 atctgggtaa aaccatcgcc attgctatca acttatttaa tccgcaaaaa attgttattg
     7261 ctggtgaaat caccgaagcc gataaagtgc tgctccctgc tattgaaagc tgcattaata
     7321 cccaagcgct gaaggcgttt cgtactaatc tgccggtggt acgttctgag ctggatcacc
     7381 gctcggcaat cggcgctttt gcgctggtaa aacgcgccat gctcaacggt attttgctcc
     7441 agcatttgct ggaaaattaa tgtgctttta tagtggcgct tattgttgtc aatattctgg
     7501 gtagtccatg accattaaaa atgtaatttg cgatatcgac ggcgtgctga tgcacgataa
     7561 cgtcgccgta ccgggtgcag cggaattttt gcacgggatt atggataaag gcctgccgct
     7621 ggtgttgctg accaactatc cttcgcagac tgggcaagat ctggcgaacc gctttgccac
     7681 tgccggtgtc gatgtgccgg atagcgtgtt ttatacctct gcgatggcga ccgccgattt
     7741 cctgcgtcgc caggaaggca agaaagcgta tgtggtgggc gaaggcgcac tgattcatga
     7801 actgtacaaa gccggtttca ctattaccga tgtgaaccct gatttcgtga ttgttggcga
     7861 aacacgttcc tacaactggg acatgatgca taaagcagcc tatttcgtcg ctaacggtgc
     7921 acgctttatc gccaccaacc cggacacggt ttttatcccg cttgtggcgc gttgtgtgca
     7981 gggattgaga aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc
     8041 atccgcgcgg cattaaacaa aatgcaggcg cattcggaag aaacagtgat tgtcggcgat
     8101 aacctgcgta ccgatattct ggcgggcttc caggccggtc tggagacgat tctggtgctt
     8161 tctggtgttt catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac
     8221 ccgtcagtcg ctgaaatcga cgttatctga ataccaccca cgtctgcgga cgtggttggc
     8281 tctgatgaca aacgccaaac tgcctgatgc gctacgctta tcaggcctac gcagctcctg
     8341 caatatattg aattttcatg cttttgtagg ccgtataagg cgttcacgcc gcatccggca
     8401 tgaacaaatc gcactttatc agcaatttgg cccccgtaaa atataaccat tcaataaaac
     8461 ccgctaaaaa ttatcgattc atcaataaat acatcctcac accagaccct ttttcaccaa
     8521 tcgacaatca ccattacgtt ttttattttt gcactgccaa atagcttgcg cacagtgctg
     8581 ctttgcggca tttttttaaa caagcaaaca caacaagcaa caaataccag gttaacggag
     8641 aaggttatgt gttcaatttt tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt
     8701 aagaaagcac tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggtatt
     8761 tatgccagcg ataacgccat tctcgctcac gaacgtctgt caattgttga cgttaacgcg
     8821 ggggcgcaac ctctctacaa ccaacaaaaa acccacgtac tggcggtaaa cggtgaaatc
     8881 tacaaccacc aggcattgcg cgctgaatat ggcgatcgtt atcagttcca gacagggtct
     8941 gactgtgaag tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg
     9001 cagggcatgt ttgcctttgc cctgtacgac agcgaaaaag atgcctacct gattggtcgc
     9061 gaccatctgg ggatcatccc actgtatatg gggtatgacg aacacggtca gctgtatgtg
     9121 gcctcagaaa tgaaatcgct ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg
     9181 agctatttgt ggagtcagga cggcgaaatc cgttcttatt atcatcgcga ctggttcgac
     9241 tttgatgcag tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat
     9301 tccgttaaaa gccatctgat gtctgatgtg ccttacggtg tgctactttc tggtggtctg
     9361 gattcctcaa ttatttccgc tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag
     9421 gaacgctctg aagcctggtg gccgcagtta cactcctttg ctgtaggtct gccgggttca
     9481 ccggatctta aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt
     9541 cacttcactg tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact
     9601 tatgatgtga ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag
     9661 gcgatgggca ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt tggcggttat
     9721 ctttacttcc ataaagcgcc caacgctaaa gaactgcatg aagagacggt gcgtaaactg
     9781 ctggccctgc atatgtatga ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg
     9841 gaagcacgcg ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg
     9901 caggataaaa tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag
     9961 tcatacctgc ccgcaagcgt ggcctggcgg cagaaagagc agttctccga tggcgtcggt
    10021 tacagttgga tcgacaccct gaaagaagtg gcggcgcagc aggtttctga tcagcaactg
    10081 gaaactgccc gcttccgctt cccgtacaac acgccgacct caaaagaagc gtatctgtac
    10141 cgcgagatct ttgaagaact gttcccactt ccgagtgccg ctgagtgcgt gccgggcggt
    10201 ccttccgtcg cgtgttcttc agctaaagcg atcgaatggg atgaagcgtt caaaaaaatg
    10261 gacgatccgt ctggtcgtgc ggttggcgtt caccagtcgg cgtataagta agaaatgcgg
    10321 tactgccccg gagaaatccg gggctgtttt agttattgcg ctgcttgttg attctgatat
    10381 gcagtaagga aatgttccgg gtttgccaca tcactgtttg acaactcatc actgtaggcc
    10441 agtatttcat agattaattt cccaccacta ttgttgaatt ccgaatttca tcctgtacga
    10501 caaaagaatg gctgtagggt ggggaaaaaa cacaaagtaa acaataattg acgaatatag
    10561 cgccacgctg ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga
    10621 ggttgacgct gcaaggctct atacgcataa tgcgccccgc aacgcagata aggtatcgcg
    10681 aaaaaaagat ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca
    10741 caggttcgaa tcccgtcgta gccaccatct tttttgcggg agtggcgaaa ttggtagacg
    10801 caccagattt aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc tcccgcacca
    10861 ttcaccagaa agcgttgtac ggatggggta tcgccaagcg gtaaggcacc ggtttttgat
    10921 accggcattc cctggttcga atccaggtac cccagccatc ttcttcgagt aagcggttca
    10981 ccgcccggtt attggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    11041 ctggttcgaa tccaggtacc ccagccatcg aagaaataat ctggctacgt agctcagttg
    11101 gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccaa
    11161 attctgaatg tgtcgaatac gttcggcaaa ttcaaaaacc aatttgttgg ggtatcgcca
    11221 agcggtaagg cacc
//
LOCUS       DAJTNM010000124        11192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTNM010000124 DAJTNM010000000
VERSION     DAJTNM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.124"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            24..383
                     /locus_tag="OO172_003379"
     CDS             24..383
                     /locus_tag="OO172_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5513438.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(503..682)
                     /locus_tag="OO172_003380"
     CDS             complement(503..682)
                     /locus_tag="OO172_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5513439.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            756..2117
                     /gene="pabB"
                     /locus_tag="OO172_003381"
     CDS             756..2117
                     /gene="pabB"
                     /locus_tag="OO172_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5513440.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2121..2699
                     /locus_tag="OO172_003382"
     CDS             2121..2699
                     /locus_tag="OO172_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5513441.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2883..4247
                     /gene="sdaA"
                     /locus_tag="OO172_003383"
     CDS             2883..4247
                     /gene="sdaA"
                     /locus_tag="OO172_003383"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5513442.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDNLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4378..5976
                     /locus_tag="OO172_003384"
     CDS             4378..5976
                     /locus_tag="OO172_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5513443.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(5980..7530)
                     /gene="yoaE"
                     /locus_tag="OO172_003385"
     CDS             complement(5980..7530)
                     /gene="yoaE"
                     /locus_tag="OO172_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5513444.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENCDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            7993..8964
                     /gene="manX"
                     /locus_tag="OO172_003386"
     CDS             7993..8964
                     /gene="manX"
                     /locus_tag="OO172_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5513445.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFIPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9027..9827
                     /gene="manY"
                     /locus_tag="OO172_003387"
     CDS             9027..9827
                     /gene="manY"
                     /locus_tag="OO172_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5513446.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9840..10691
                     /gene="manZ"
                     /locus_tag="OO172_003388"
     CDS             9840..10691
                     /gene="manZ"
                     /locus_tag="OO172_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5513447.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10746..>11192
                     /locus_tag="OO172_003389"
     CDS             10746..>11192
                     /locus_tag="OO172_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5513448.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
BASE COUNT         2748 a         2826 c         2825 g         2793 t
ORIGIN      
        1 cgttataatc cctttgatat tagatgcaaa ttaaggtcat atatagcctt atcgataaca
       61 tggttaattt taaggataag aatatgcctg ctgtaataga taaagccctg gatttcattg
      121 gtgccatgga tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg gcgaagggaa
      181 tctttaaata tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt acggcgcgag
      241 ctgaccagga aggctggaac cctgggttca cggaaaaaat ggttggatgg gcaaaaaaaa
      301 tggagacagg tgaacgctct gtgattaaaa atcctgaata cttttcaaca tatatgcagg
      361 aagaactaaa agcactggtc tgagttaaat ttatatcagc ataaatgggt caggacgctt
      421 ttaatcacat ataaaaagcg tcctttttct cacaatcaaa tgatcatcgg cttctataac
      481 gaaatcgata cggattagtg tgttactcgt cttcatcctc aaaacgcgcc acgattagct
      541 cgccggagtg gttggcacgc agctcttctg ccaccagcgc gattgcctgg ccgctactca
      601 tcccttgtgc catcagttcc tggatccgct cgacagcttt ttgctgctgt tcatgggtga
      661 gtgaaggtaa acctgcaaac attgttaact cctgctaaat tgttggcgct aattatttca
      721 tgctacccgg cacatagcca gtagagtcag gactgatgaa gacgttatct cccgctgtga
      781 ttactttacc ctggcgtcag gacgccgctg aattttattt ctcccgctta agccacctgc
      841 cgtgggcgat gcttttacac tccggctatg ccgatcatcc gtatagccgc tttgatattg
      901 tggtcgccga tccgatttgc actttaacca ctttaggtaa agaaaccgtt gttagtgaaa
      961 gcgaaaaacg cacaacgacc actgatgacc cgctacaggt gctccagcag gtgctggatc
     1021 gcgcagacat tcgcccaacg cataacgaag atttgccatt tcagggcggc gcactggggt
     1081 tgtttggcta cgatctgggc cgccgttttg agtcactgcc agaaattgcg gaacaagata
     1141 tcgttctgcc ggatatggca gtgggtatct acgattgggc gctcattgtc gaccaccagc
     1201 gtcatacagt ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc tggctggaaa
     1261 gccagcaatt ctcgccgcag gaagatttca cgctcacttc cgactggcaa tccaatatga
     1321 cccacgagca gtacggcgaa aaatttcgcc aggtacagga atatctgcac agcggtgatt
     1381 gctatcaggt gaatctcgcc cagcgttttc atgcgaccta ttctggcgat gaatggcagg
     1441 cattccttca gcttaatcag gccaaccgcg cgccatttag cgctttttta cgtcttgaac
     1501 agggtgcaat tttaagcctt tcgccagagc ggtttattct ttgtgataat agtgaaatcc
     1561 agacccgccc gattaaaggc acgctaccac gcctgcccga tcctcaggaa gatagcaaac
     1621 aagcagaaaa actggcgaac tcagcgaaag atcgtgccga aaatctgatg attgtcgatt
     1681 taatgcgtaa tgatatcggt cgtgttgccg tagccggttc ggtaaaagta ccagagctct
     1741 tcgtggtgga acccttccct gccgtgcatc atctggtcag cactataacg gcgcgactac
     1801 cagaacagtt acacgccagc gatctgctgc gcgcagcttt tcctggtggc tcaataaccg
     1861 gggctccgaa agtacgggct atggaaatta tcgacgaact ggaaccgcag cgacgtaatg
     1921 cctggtgcgg cagcattggc tatttgagct tttgcggcaa catggatacc agcattacta
     1981 tccgcacgct gactgccatt aacggacaaa tatactgctc tgcgggcggt ggaattgtcg
     2041 ccgatagcca ggaagaagcg gaatatcagg aaacttttga taaagttaat aagatattac
     2101 gccaactgga gaagtaaaac gtggaatacc gtagcctgac gcttgatgat tttttatctc
     2161 gctttcaact tttgcgcccg caaattaacc gggaaaccct aaatcatcgt caggctgccg
     2221 tgttaatccc catcgtccgt cgaccacaac cggggttgtt gctgactcag cgttcaattc
     2281 atctgcgtaa acacgctgga caagtggcat tccctggagg tgcagtcgat gacacggacg
     2341 catcagttat cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata ccgccttccg
     2401 ccgttgaagt tatcggcgtg ctgccgcccg tcgatagcgt aactggctat caggtaaccc
     2461 cagtggtcgg cattatcccg cccgatctgc cgtatcgcgc cagtgaagat gaagtctcgg
     2521 cggtgtttga aatgccgctc gcccaggcat tacatctggg tcgttatcac cctttagata
     2581 tctaccgccg tggcgattca catcgggtat ggctgtcctg gtacgaacag tattttgtat
     2641 ggggaatgac cgcaggcata attcgtgagc tggcgctgca aattggtgtg aacccctgac
     2701 tatacttatc tttacatcta caaaacacta cttgagacaa tcatcgcaat attagttaaa
     2761 tcgcggtttt tgattagttt aattcatgtg aatagttaag ccagtcgccg cgttccctct
     2821 tacactatgc gctgttatta gttcgttact ggaagtccag tcaccttgtc aggagtatta
     2881 tcgtgattag tctattcgac atgtttaagg tggggattgg tccctcatct tcccataccg
     2941 tagggcctat gaaggcgggt aaacagttcg tcgataatct ggtcgaaaaa ggcttactgg
     3001 atagcgttac tcgcgttgcc gtggacgttt atggttcact gtcgctgacg ggtaaaggcc
     3061 accacaccga tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc accgtggata
     3121 tcgacagtat tcccggtttt attcgcgacg taaaagagcg cgaacgtctg ctgctggcac
     3181 agggacggca tgaagtggat ttcccgcgcg acaacgggat gcgttttcat aacggcaacc
     3241 tgccgctgca tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa gtcgtctaca
     3301 gcaaaactta ttattccatc ggcggcggtt ttatcgtcga tgaagaacac tttggtcagg
     3361 atgctgccaa cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa ctgctcgcgt
     3421 actgtaatga aaccggctat tcgctgtctg gtctcgctat gcagaacgaa ctggcgctgc
     3481 acagcaagaa agagatcgac gagtatttcg cgcatgtctg gcaaaccatg caggcatgta
     3541 tcgatcgcgg gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg ccacgtcgtg
     3601 cgtctgccct gcgccggatg ttagtttcca gcgataaact gtctaacgat ccgatgaatg
     3661 tcattgactg ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc gccggtggtc
     3721 gtgtggtaac tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg ctggcttact
     3781 atgaccactt tattgaatcg gtcagcccag acatctatac ccgttacttt atggcagcgg
     3841 gcgcgattgg tgcattgtat aaaatgaacg cctctatttc cggtgcggaa gttggttgcc
     3901 agggcgaagt gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa ctgctgggcg
     3961 gtagcccgga acaggtttgc gtggcggcgg aaattggcat ggaacacaac cttggtttaa
     4021 cctgcgaccc ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat gccattgcct
     4081 ctgtgaaggc gattaacgcc gcacggatgg ctctgcgccg caccagtgca ccgcgcgtct
     4141 cgctggataa ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac gccaaatacc
     4201 gcgaaacctc acgcggtggt ctggcaatca aagtccagtg tgactaatac ttcttactcg
     4261 cccatctgca acggatgggc gaatttatac ccgctttctc gtctgctgta atattcccca
     4321 ctacacttcc actgttgcgt caggcgtttg tcgccatacg cttacagggt ggcccgcatg
     4381 caaaaagcac agcggatcat taaaacctat cgccgtaatc gaatgattgt ttgtacgatt
     4441 tgcgcactcg ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca gcgtaactta
     4501 aatcaacaac gggtagtaca attcgccaat cacgctgtag aggaattaga taaagtactg
     4561 cttcccctac aggcaggtag cgaagtcttg cttccgctga ttggtctgcc ctgctctgtc
     4621 gcccatttgc cattacgtaa acaggcggca aaactccaaa ctgtgcgatc cattggcctg
     4681 gtgcaagacg gcacacttta ttgctccagc atttttggtt atcgcaatgt gcccgtcgtg
     4741 gacattctgg ctgaacttcc tgcaccgcaa ccacttttac gcctgacgat cgaccgtgcc
     4801 ctgattaaag gcagtccggt tttgattcaa tggacgcctg cagcaggcag tagcaatgct
     4861 ggggtcatag agatgattaa catcgactta ctgacggcaa tgctccttga gccacaactg
     4921 ccgcaaatca gtagcgccag cctgacggtg gacaaacggc atttgctcta tggtaatggg
     4981 ctggtagatt cccttccgca acctgaagac aatgaaaact atcaggtttc ttcgcaaagc
     5041 tttcctttta ccattaacgt aaatggtccg ggggctacgg cgctggcatg gcactatctt
     5101 ccaacacaat taccgctggc ggtgctgcta agtttactgg tgggctatat cgcctggctg
     5161 gcgaccgctt accggatgag cttttcccgc gaaattaatc tgggcctggc gcaacatgag
     5221 ttcgaattgt tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat tggtgtagag
     5281 attttgctgc gctggaataa tccgcgtcag ggctggattt caccggatgt gtttattcct
     5341 atcgcggaag aacatcattt aattgtgcca ctgacccgct atgtgatggc agaaaccatt
     5401 cgtcagcgcc atgttttccc gatgagtagt cagtttcatg ttggcattaa cgtcgcaccc
     5461 agccattttc gccgtggtgt gctgataaaa gatctcaatc agtactggtt tagcgctcac
     5521 ccgattcagc aactgatcct cgaaatcacc gaacgcgatg ccttactgga tgttgattat
     5581 cggattgccc gcgagctgca tcgtaaaaac gtcaaactgg cgattgatga cttcggcacc
     5641 ggcaacagct cgttttcctg gcttgaaaca ttacgtcctg acgtgctgaa aattgataag
     5701 tcatttaccg cagctatagg ttctgacgcg gttaactcga cggtgaccga tatcatcatc
     5761 gcgctggggc aaagactgaa tattgaactg gtggcggagg gcgtggaaac gcaagaacag
     5821 gcgaagtatt tgcgccgtca tggcgtgcat attttgcaag ggtatttgta cgcacagccg
     5881 atgccgctac gtgattttcc caaatggctg gcgggcagcc aaccgccgcc cgcccggcat
     5941 aatggacata tcacgcccat tatgtcgtta cgttaacgct tactcatctt catcgtgcgc
     6001 cggttgctct ttaacaatgc gaaccagatc aacacgataa tcattggctt caaggatggt
     6061 gatatgcagt ggccctacat caatcacatc gcccacacgg ggaatgtgac catttgccga
     6121 gatcacgagg cccgcgaccg tcgcgatatc atcgtcatcg gcaaggtgct caacatcaag
     6181 cgcctgctgc aaggcatgca aatctgtacc gccttttacc agccagccgt taccatcggt
     6241 aatgatttcc ggcgtttcgt cagcgtccgg gaattcaccc gcaatggctt ccagcacatc
     6301 cagcggcgtg accaagcctt gtaccacacc aaactcgttg gtcacgataa caaagctccc
     6361 gcgagcgcga cgcagcacgc ccagcaggtt gatcggatcg agggtttccg ggacgataat
     6421 cgccggagac gccgaagcaa tcgccgccac atcaacgccc tcttccagcg ccaccagcag
     6481 ttctttagca cgtacgatac cgatgatttc atccagttca ccgcgacata ccgggaacag
     6541 actgtgcggt gaagagagca gttgctcgcg gatttcatcg accccgagat tagcgtcaac
     6601 ccagcttatt tcaccgcgcg gcgtcatgat cccgcgcaga gaacgcgacg ccagcgtcag
     6661 tacgccgtta atcatgtacc gttcttcttc ggcaaatgca ccttccggga tcggcatcgg
     6721 gttatcggca tcgtgctgaa cattggcctg acgtttcccg cccatcaaac gcaggatggc
     6781 atcggcagta cgcgctcgca gcggcaaagt cgactggtga cgaataaagt tgcgacgcgc
     6841 aatctggtta aacacttcga taatgatcga gaagccaatc gcggcataca ggtaaccttt
     6901 cggaatgtgg aaaccgaaac cttctgccac cagactcaga ccaatcatta acaggaagct
     6961 cagacagagc accaccaccg tggggtgctg gttaacgaat cgcgtcagcg gtttcgatgc
     7021 cagcaacata accgccatcg caatcactac cgccgccatc atcaccggca gatggttaac
     7081 catccctact gcagtaatta ccgcatccaa cgagaagacg gcgtcaagga tgacaatctg
     7141 tgtgacgacc acccagaaac tggcgtagcc tttaccgtgg ccggaatcat gatcgcagtt
     7201 ttccagccgt tcatgcagtt cggttgttgc tttgaacagc aagaatatcc ccccgaacaa
     7261 cataatcaga tcgcgtccgg agaaggagaa atccatgacg gtaaatagcg gtttggtcag
     7321 cgtgaccatc catgaaatca gcgacagcag ccccagacgc ataatcagcg ccagtgataa
     7381 ccccagcaaa cgcgctttat cgcgttgttt tggcggcagt ttgtcagcaa gaatggcgat
     7441 gaagaccagg ttatcgatac ccagcacaat ttcgagaaca acaagcgtga gtagccccgc
     7501 ccaaattgag gggtccatta agaattccat gacaagctcc tgcttaagga atagctattc
     7561 gacgccagaa ataatgcagg cgtaacgaca aaatgcaaac gaaaggtgcg gcatagagtg
     7621 ccagaaaggc aggcgttaaa aggcctgatg ctgaaatgac gtcggtgacg atccatactg
     7681 cgggctactg ccctatactc catggttgtt aaacgggagt taaacatatc agagacgcct
     7741 ctgatttggc aaagatttac cttcctttgc aaacgaatgt gacaaggata ttttaccttt
     7801 cgaaatttct gataatcgaa agttaaatta cggatcttca tcacataaaa taattttttc
     7861 gatatctaaa ataaatcgcg aaacgcaggg gtttttggtt gtagccctta tctgaatcga
     7921 ttcgattgtg gacgacgatt caaaaataca tctggcacgt tgaggtgtta acgataataa
     7981 aggaggtagc aagtgaccat tgctattgtt ataggcacac atggttgggc tgcagagcag
     8041 ttgcttaaaa cggcagaaat gctgttaggc gagcaggaaa acgtcggctg gatcgatttc
     8101 attccaggtg aaaatgccga aacgctgatt gaaaaataca acgctcagtt ggcaaaactc
     8161 gacaccacta aaggcgtgct gtttctcgtt gatacatggg gaggcagccc gttcaatgct
     8221 gccagccgca ttgtcgtcga caaagagcat tatgaagtca ttgcaggcgt taacattcca
     8281 atgctcgtgg aaacgttaat ggcccgtgat gatgacccga gctttgatga actggttgcg
     8341 ctggcagtag aaacaggccg tgaaggcgtg aaagcactga aagccaaacc ggttgaaaaa
     8401 gccgcgccag cacccgctgc cgcagcacca aaagcggctc caactccggc aaaaccaatg
     8461 gggccaaacg actacatggt tattggcctt gcgcgtatcg acgaccgtct gattcacggt
     8521 caggtcgcca cccgctggac caaagaaacc aatgtctccc gtattattgt tgttagtgat
     8581 gaagtggctg cggataccgt tcgtaagaca ctgctcaccc aggttgcacc tccgggcgta
     8641 acagcacacg tagttgatgt tgccaaaatg attcgcgtct acaacaaccc gaaatatgct
     8701 ggcgaacgcg taatgctgtt atttaccaac ccaacagatg tagagcgtct cgttgaaggc
     8761 ggcgtgaaaa tcacctctgt taacgtcggt ggtatggcat tccgtcaggg taaaacccag
     8821 gtgaataacg cggtttcggt tgatgaaaaa gatatcgagg cgttcaaaaa actgaatgca
     8881 cgcggtattg agctggaagt ccgtaaggtt tccaccgatc cgaaactgaa aatgatggat
     8941 ctgatcagca aaatcgataa gtaacgtatt gtgttgatta tcattcagtt ttcacactta
     9001 agtcttacgt aaacaggaga agtacaatgg agattaccac tcttcaaatt gtgctggtat
     9061 ttatcgtagc ctgtatcgca ggtatgggat caatcctcga tgaatttcag tttcaccgtc
     9121 cgctaatcgc gtgtaccctg gtgggtatcg ttcttgggga tatgaaaacc ggtattatta
     9181 tcggtggtac gctggaaatg atcgcgctgg gctggatgaa catcggtgct gcagttgcgc
     9241 ctgacgccgc tctggcttct atcatttcta ccattctggt tatcgcaggt catcagagca
     9301 ttggtgcagg tatcgcactg gcaatccctc tggccgctgc gggccaggta ctgaccatca
     9361 tcgttcgtac tattaccgtt gctttccagc acgctgcgga taaggctgct gataacggca
     9421 acctgacagc gatttcctgg atccacgttt cttctctgtt cctgcaagca atgcgtgtgg
     9481 ctattccggc cgtcatcgtt gcgctgtctg ttggtaccag cgaagtacag aacatgctga
     9541 atgcgattcc ggaagtggtg accaatggtc tgaatatcgc cgggggcatg atcgtggtgg
     9601 ttggttatgc gatggttatc aacatgatgc gtgctggcta cctgatgccg ttcttctacc
     9661 tcggcttcgt aaccgcagca ttcaccaact ttaacctggt tgctctgggt gtgattggta
     9721 ctgttatggc agtgctctac atccaactta gcccgaaata caaccgcgta gccggtgcgc
     9781 ctgctcaggc agctggtaac aacgatctcg ataacgaact ggactaacag gtgagcgaaa
     9841 tggttgatac aactcaaact accaccgaga aaaaactcac tcaaagtgat attcgtggcg
     9901 tattcctgcg ttctaacctc ttccagggtt catggaactt cgaacgtatg caggcactgg
     9961 gtttctgctt ctctatggta ccggcaattc gtcgcctcta ccctgagaac aacgaagctc
    10021 gtaaacaagc tattcgccgt cacctggagt tctttaacac ccagccgttc gtggctgcac
    10081 cgattctcgg cgtaaccctg gcgctggaag aacagcgtgc taatggcgca gagatcgatg
    10141 acggtgctat caacggtatc aaagtcggtt tgatggggcc gctggctggt gtaggcgacc
    10201 cgatcttctg gggaaccgta cgtccggtat ttgcagcact gggtgccggt atcgcgatga
    10261 gcggcagcct gttaggtccg ctgctgttct tcatcctgtt taacctggtg cgtctggcaa
    10321 cccgttacta cggcgtagct tatggttact ccaaaggtat cgatatcgtt aaagatatgg
    10381 gtggtggctt cctgcaaaaa ctgacggaag gggcgtctat cctcggcctg tttgtcatgg
    10441 gggcattggt taacaagtgg acacatgtca acatcccgct ggtggtttcg accatcaccg
    10501 ggcaggatgg acagacccgt gtcaccaccg tgcaaactat tcttgaccag ttaatgccag
    10561 gccttgtacc actgctgctg acatttgctt gtatgtggct gctgcgcaaa aaagttaacc
    10621 cgctgtggat catcgttggc ttcttcgtca tcggtatcgc tggttacgct tgcggcctgc
    10681 tgggactgta agactgttgt acactaccgg ggccttttgg ccccgttttt ttatctggag
    10741 gattaatgac aatcacggac ctggtactga ttcttttcat cgccgcactc ctggccttcg
    10801 cgatctacga tcagttcatc atgccccgcc gtaacggccc caccctgctg gcaattcctt
    10861 tgctccgccg tggtcgcatc gatagcgtta tcttcgtcgg attgattgtc attcttatct
    10921 ataacaacgt cacgaatcat ggcgcgctaa taacaacatg gttattaagt gcactggctc
    10981 tgatgggttt ttatatattc tggatccgca ttccgaagat catctttaaa caaaaaggtt
    11041 ttttcttcgc caatgtctgg attgaatata gccgaatcaa agcgatgaac ttgtcggaag
    11101 atggcgtgtt ggtgatgcaa ttagaacagc gtcggctgtt aatccgcgtt cgaaatatcg
    11161 acgatctgga aaaaatttat aagcttctcg ta
//
LOCUS       DAJTNM010000125        10813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTNM010000125 DAJTNM010000000
VERSION     DAJTNM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.125"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            126..1823
                     /gene="maeA"
                     /locus_tag="OO172_003390"
     CDS             126..1823
                     /gene="maeA"
                     /locus_tag="OO172_003390"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5513449.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1957..2967
                     /gene="adhP"
                     /locus_tag="OO172_003391"
     CDS             1957..2967
                     /gene="adhP"
                     /locus_tag="OO172_003391"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5513450.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3113..3397
                     /locus_tag="OO172_003392"
     CDS             3113..3397
                     /locus_tag="OO172_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5513451.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3625..4278)
                     /gene="fdnI"
                     /locus_tag="OO172_003393"
     CDS             complement(3625..4278)
                     /gene="fdnI"
                     /locus_tag="OO172_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5513452.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4271..5155)
                     /gene="fdnH"
                     /locus_tag="OO172_003394"
     CDS             complement(4271..5155)
                     /gene="fdnH"
                     /locus_tag="OO172_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5513453.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5168..8215)
                     /gene="fdnG"
                     /locus_tag="OO172_003395"
     CDS             complement(5168..8215)
                     /gene="fdnG"
                     /locus_tag="OO172_003395"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7628..7630),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5513454.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8447..9328
                     /gene="yddG"
                     /locus_tag="OO172_003396"
     CDS             8447..9328
                     /gene="yddG"
                     /locus_tag="OO172_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5513455.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9587..10687
                     /gene="ompC"
                     /locus_tag="OO172_003397"
     CDS             9587..10687
                     /gene="ompC"
                     /locus_tag="OO172_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5513456.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2653 a         2776 c         2679 g         2705 t
ORIGIN      
        1 gattaaacac cattgcctgc gcaatggtgt ttttatctgc tttatacttg gggacgacgc
       61 cctggcggta aagcaaagac gataaaagcg tgccagggat ggatattcaa aaaagagtga
      121 gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg
      181 tactgctgga atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta
      241 acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac
      301 gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta
      361 acatccagga caccaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga
      421 tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct
      481 accgccgttc acgcggcgtg tttatctctt accagaaccg ccacaatatg gacgatattc
      541 tgcaaaacgt gccgaaccat aatattaaag tgattgtggt gactgacggt gaacgtattc
      601 tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct
      661 ataccgcctg tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggaa
      721 cgaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca
      781 ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc
      841 cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc
      901 gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcactgcg gcggtaacag
      961 tcggcacact gatcgcagcc agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg
     1021 tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc
     1081 agcgcgaagg attaagcgag gaagcagcgc ggcagaaagt ctttatggtc gatcgctttg
     1141 gcctgctgac ggacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc
     1201 gcgaaaacct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc
     1261 gcaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag
     1321 agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc
     1381 cgacgtctcg cgtggaagcc acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc
     1441 tggtcgccac tggcagcccg tttaatcctg tggtatggaa agataaaatc taccctatcg
     1501 cccagtgtaa caacgccttt attttcccgg gcatcggcct gggtgttatt gcttccggcg
     1561 cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacacttgct cagtattcgc
     1621 cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct
     1681 cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtcaaaa
     1741 cgtctgctga agctttgcaa caagccattg acgataattt ctggcaagcc gaataccgcg
     1801 actaccgccg tacctccatc taagcctgca cccggtagtg aaggctaccg ggctatttcc
     1861 ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca
     1921 cctgctatgc agaacatcat ccgaaaagga ggaactatga agactgcagt tgttacgaag
     1981 gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg
     2041 ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt
     2101 ggtgacaaaa ccggcgtaat tctgggccat gaaggcatcg gtgtggtggc agaagttggc
     2161 ccaggtgtca cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ttacgaagga
     2221 tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat
     2281 gccggataca gcgttgatgg cgggatggcg gaagagtgca tcgtggtcac cgattacgcg
     2341 gtaaaagtgc cagatggtct ggactcggcg gcgcccagca gcattacctg tgcgggggtc
     2401 accacctaca aagccgttaa actgtcaaaa attcgtcccg gtcagtggat tgctatctac
     2461 ggtcttggcg gtctgggtaa cctcgccctg caatacgcaa agaatgtctt taacgccaaa
     2521 gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat gggtgcagat
     2581 ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc
     2641 gctcacgctg cggtggtaac agcagtagct aaagctgcgt ttaactcggc agttgatgct
     2701 gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat gagcctggat
     2761 atcccacgtc ttgtgctgga tggcattgaa gtggtcggtt cgctggtcgg cacgcgccag
     2821 gatctaaccg aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg
     2881 cgtccgttag cggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc
     2941 cgtatggtga ttgatttccg tcactaagag gtctttgctg cgactcccat gttcgggtcg
     3001 cagcatcgca cactctccaa catgaaatgg ctaaatggat tattgttgcg taggaaggaa
     3061 caataatgat agtgttccca gagaagtaac gctactgaga gtaaagacac taatgaaaat
     3121 ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag
     3181 cctgcgcgag tttgccagag caatggaaat tgcgccctca acggcaagcc gattgctgac
     3241 cggaaaagca gctttgacgc cagaaatggc aattaaactt tccgtggtga tcggcagttc
     3301 gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag aaaaaacggt
     3361 ggttgtgtcg cgattacgcc gtctggttac gcaataaaaa atggcacctt ataaagcgcc
     3421 atttgaaact gatgacaaac gcaacattgc ctgatgcgct acgcttatca ggcctacacg
     3481 ttcacttcaa tctattgtat ttgaagggtt ttcatgccgg ataaggcatt cacgacgcat
     3541 ccgggatgaa caaagcccac gttgtctaca atatgaaaat ggcgcctttt acagcgccat
     3601 tttcagctta aagttcaaaa gagattatat cccttcttca ctctcttttt tcgcttctgc
     3661 cttctcgatt tcacgatacc agcgcggatg gtgtttcttc gcccagcgac ggctcacctt
     3721 cccttcgatc atccctttaa tcgacccttt cacccagaat gccatataca tatggatcag
     3781 gatggcgtgg atcaggatga tacccgcagc cgcgtggatc agcaggctgt agcgaacaac
     3841 ctgcatcggg aagtactgcg caaagtacgg acgccagata atcaccccgg tcaccagcag
     3901 cacgaaaatc atgctcatga tcgaccagaa catcatcttt tgcccggcgt tgtacttacc
     3961 aacatccgcc actttgtgct cattgccttt taatacttcg acaatgttca acagccacgg
     4021 aatatctttc ttatccggga tgttgtgatg cacaaaacgc acaaacataa acatcagtgc
     4081 gacgaaaatc gcaatgccga agaacgggtg caaaatgcgt cccatctgcg gcgtaccgaa
     4141 ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa atcccggaca gcgccaccag
     4201 gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca ataaatttgg tgcgcacaat
     4261 cattttcgac ttactcatga tgatcctcct cgtcatcgtc cacttcctta ttcgggccaa
     4321 taccgatgta gtggaaaatc aacccggcaa aagtggcaat aaagccagcc gctgccagcg
     4381 gtttcaacgc gcctttccac aggcttaccg aggtgtcgat cttcggatct ttcggcagac
     4441 cgtgatacag ctccggctga tcggcgtgat gcagcacgta cataacgtgc gtaccaccga
     4501 ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg cgctttcagt ttcgccacgc
     4561 gctgttccgc cagctccagc atctccttct tggtgccgaa gtggatagcc ccggtcggac
     4621 aggttttcac acaagccggt tcctggccga cgctgacgcg atcgacgcag agcgtgcatt
     4681 tatatacccg gttatcctct ttgttgaggc gcggaatatt aaacggacac ccggcaatgc
     4741 agtaaccaca gccgatgcag ttttccgact ggaaatcgac aatcccgtta gcgtactgaa
     4801 tgattgcacc agcagacggg cacgccttca ggcagcccgg atcttcacag tgcatacagc
     4861 cgtctttacg gatcagccac tccagcttgc cattctgttc ggtttcgcta aagcgcatca
     4921 ccgtccagga cttggcgctc agatcggcgg ggttatcgta aacaccgacg cagtgcccca
     4981 cttcatcacg gatgtcgttc cactccgaac acgccacctg acaggcttta cagccgatac
     5041 aggtggaaac gtcgataagt ttcgcgactt ctgctttgta atcacgcacc tgagaaggcg
     5101 gcgtgatgga gttagttgcg gaccttttga taatatcctg cgtttccata gccatctgtt
     5161 cgccccctta cgccttctcg atgttgacta agaacgcttt atattccggc gtttgcgagt
     5221 ttgcatcacc gacattcggc gtcagagtgt tagcgatata acctttacgc gcgacaccct
     5281 caaagcccca gtggattgga atacccaccg tttcaacctg ctgaccgttt acattcagcg
     5341 gtttcagacg gcgcgttacc actgccaccg cgcggataaa gcctcgcttg ctggagacag
     5401 tgacacgatc gccattatta atgcctttcg ccgccgcctg cgtttcgctg atttccacaa
     5461 actgttccgg ctgagcaatt gcgttgagca acgcgtgctt ggtccaggtg tggaagtgct
     5521 cggtcagacg ataggtcgta cccacatacg ggaactgctc ttttttaccc atccgcagcg
     5581 cgtcttgttc atacagacga acaaccgggt tagacaccac gttcggatgc agcgggttag
     5641 tgcccagcgg cgtttcaatc ggctcgtagt gttccgggaa cggaccttcc gccattttgt
     5701 tgatggcaaa caggcgtccc atcccttccg gctgcataat aaacggcccg gttggcgtac
     5761 ccggtgcggc attgccgaag tccggaatat cgttacccgt ccacttgctg ccgttccact
     5821 ggatcagcat ccgtttcgga tcccacggtt taccgttgat atccgccgaa gcacggttgt
     5881 agagtacgcg acggttgagc ggccacgccc aggcccatcc cagcgtattc cccagaccgg
     5941 acgggtcgga gttatcgcgg tttgccatct ggttaccctg ctctgtccag ctaccggtgt
     6001 agatccagca agaagatgcg gttgtaccgt catcacgcag atgcgcaaag ctactcagca
     6061 actgaccttt cttcgcaatc agcacgccat tagcatcata gagatcttcc aacgcatagc
     6121 cgttgttctc tttagctact tcgtcagatt gcggttcgtg cggctgcttg tagttccagc
     6181 tcatcttcat cagcggttct acgcctttac caccttcggc ctggtacaac tcgcgcagat
     6241 gatggtagat acccgccaga atttcgccgt cgttacgcgc ttcgcccggc gcgtcctgac
     6301 ctttccagtg ccactgcaac cagcgaccgg agttagcgat agaaccatcc tcttcagcaa
     6361 agcaggtcga aggcagacgg aatacttcag tctgaataga cgccggatcg acatcgttcg
     6421 actcgccgtg gttctgccag aaggtagagg tttcagtcac cagcggatcg ataaccacca
     6481 tgtacttcag tttgctcagg cagctcacca ctttgttttt atccgggaag gacgcaaccg
     6541 ggttaaagcc ctggcagaaa taaccggtga ctttgccttc atccatcatg ttgaaatact
     6601 tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc atagccccag ttgttctctt
     6661 tctgcgcggc atcgccatag aaagatttca tcaggctaac gaagaacttc ggatagttgc
     6721 tccagtagtt cacctgatca gccagcgtcg ctttcggcgt gttcgcttcc agatacgact
     6781 gcaaatcaac ctgtttttct gacggcagcg tcagataacc tggcaggctg gtagagagca
     6841 gacctaagtc agtcagcccc tgaatgttgg agtgaccacg caatgcgttc acgccgccac
     6901 cggccatacc catgttaccg agcagcaact ggatcatcgc catagtacgg atgttctgcg
     6961 cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa ggtggttgtg cgatccggtg
     7021 cgctggtgga ggccagcact tcacacactt tcaggaagtc ggcttttggc gtaccacaga
     7081 tgttttcaac aacgtccggc gtgtagcggg aaacgtgcgc tttcagcagg ttccacacac
     7141 agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc gttttcatcg aactgatagt
     7201 tccaggacga tttgtcgtac tggcgttttt cagcgtcata accgctgaac aggccatctt
     7261 cgaaagcaaa atcatcacgc accagtaggc tggcgttggt gtaatgttta acgtattcgg
     7321 cgttgatttt gttgttttcg atcaggtagc gcaaaacgcc agacaggaac gtaatgtccg
     7381 taccggaacg aataggtgcg taaatatccg ccacagaagc ggtacgcgta aaacggggat
     7441 cgacaacgat caaggttgca tcgttgttgt ttttcgcttc catcgcccag cggaaaccga
     7501 cgggatgcgc ttcagcagcg ttaccgccca ttaccattac gacgttagcg tttttgatat
     7561 ccacccagtg gttggtcata gcacctcgac caaatgttgg agcaagactt gctaccgttg
     7621 gtccgtgtca gacgcgcgcc tggttgtcta ccgccagcat cccgagggag cgggcaaatt
     7681 tctgggtcag catcccggtt tcgttgctgg caccggaggc acacagcata ccggtagaaa
     7741 gccaacggtt taccgttacg ccctgctcgt tcttttcaat aaagttagcg tcacggtcag
     7801 ctttcatcag cttcgcaata cgggagaatg cttcttccca gctaatgcgc tgccatttgt
     7861 cagaacctgg cgcacgatat tccgggtagc gcagacggtt ttcactgttg acgtaatcca
     7921 gcaaaccggc ccctttcggg cacagcgcac cacggcttac cggatgatcc gggtcacctt
     7981 caatgtgata aatcgcttct ctggcgtttt ttgcgccatc acccaggcta tacatcaata
     8041 gcccgcaacc tacggaacag tatgtgcagg tgttacggat ctctttagcg cgtaataatt
     8101 tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa gcccaatgcc gctaccgttg
     8161 ttccagccat acctcccgcg cagattttaa aaaattgtct gcgactgacg tccattgctt
     8221 tcctcttttt tcaggggtat tactgcgcga ggaaactaac agtaaaattc tgaatctttt
     8281 tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa tccgcctgcg caaattgacc
     8341 tacctcaata gcggtagaaa aacgcaccac tgcctgacag gccagttaaa aaaatgctat
     8401 aaaattcagc ttaattttta acggcaagag agacaaaaca gcgagcatga cacgacaaaa
     8461 agcaacgctc atagggctaa tagcgatcgt cctgtggagc acgatggtag gattgattcg
     8521 cggtgtcagc gaggggctcg gcccggtcgg cggcgcagct gctatctatt cattaagcgg
     8581 gctgctgtta atcttcacgg ttggatttcc gcgtattcgg cagattccga aaggctattt
     8641 actcgccggg agtctgttat tcgtcagcta tgaaatctgt ctggcgcttt ccttagggta
     8701 tgcggcgacc cgtcatcagg cgattgaagt gggtatggtg aactatctgt ggcccagcct
     8761 gacaattctc tttgccattc tgtttaatgg tcagaaaacc aactggctga ttgtaccagg
     8821 attattatta gccctggtcg gcgtctgctg ggtgttaggc ggtgagaatg ggttacatta
     8881 tgatgaaatc atcaataata tcaccaccag cccattgagt tatttcctgg cgtttattgg
     8941 tgcgtttatc tgggcagcct attgcacagt aacgaataaa tacgcacgcg gatttaacgg
     9001 aattaccgtt tttgtcctgc taacgggagc atgtctgtgg atttactatt ttctcacgcc
     9061 acaaccagaa atggtattta gcacgcccgt catgattaaa ctcatctctg cggcatttac
     9121 cttaggattt gcttatgctg catggaatgt cggtatattg catggcaatg tcaccataat
     9181 ggcggtaggc tcgtatttta cgcctgtact ttcctcagcg cttgcagccg tgctgctcag
     9241 cgccccgctg tcgttctcgt tctggcaagg cgcgctaatg gtctgcggcg gttccctgct
     9301 ctgctggctg gcgacacgtc gtggttaaaa taattgccgg gtctacggcc cggcagttaa
     9361 tcagataaat tccgacaata ttaaaaaata aatttcaaat accagtaatt caccttatat
     9421 ctcatttcga cttcgatcac atatcgataa ataatattat ttcatctatt tttctcattg
     9481 tttttaatca ccattctatt ttaaaaacag tatgtcattt ataaatgacg ccgtttaaaa
     9541 ttcgtttaga aaatacgttt aacgataatt aaaaggattc tgttaaatga aattaaaaat
     9601 agttgcggtg gttgtaactg gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta
     9661 taacaaggat ggtaataaac tcgaccttta tggcaaggtt accgctctac gttattttac
     9721 tgatgataag cgtgacgatg gtgataaaac ttatgcccgt ctcggcttta aaggagaaac
     9781 gcaaatcaat gatcaaatga ttggttttgg tcactgggaa tatgatttta aaggctataa
     9841 cgatgaagcc aacggctcgc gcggcaacaa gacccgtctg gcctatgctg gtttaaaaat
     9901 tagtgaattt ggctctctgg actatggtcg taactacggt gtcggctacg acattggttc
     9961 atggaccgat atgttgccag aatttggtgg cgatacctgg agtcagaaag atgtcttcat
    10021 gacataccgt accaccggtc tagcaaccta tcgcaactac gatttctttg gcttaattga
    10081 agggctgaac tttgccgcgc aatatcaagg caaaaatgaa cgtactgaca acagtcatct
    10141 ttatggtgct gactacacgc gtgccaacgg tgacggtttc ggtatctcct caacttatgt
    10201 ttatgatggc tttggtatcg gtgcggtata taccaaatcc gatcggacaa atgcgcagga
    10261 aagagccgct gctaatcctc tcaatgcctc cggtaagaat gcagaactat gggccacagg
    10321 tataaagtat gatgccaaca acatctactt tgcagctaat tacgctgaaa cattaaacat
    10381 gaccacctat ggggatggtt atatctctaa caaagcacaa agttttgaag tggtggcgca
    10441 atatcaattc gacttcggct tgcgcccatc actcgcttac ctgaaatcga aaggcagaga
    10501 tctgggccgc tacgccgacc aggacatgat tgagtatatc gacgttggtg cgacgtattt
    10561 cttcaacaaa aatatgtcga cctatgttga ttataaaatc aacctgattg atgaaagcga
    10621 ctttacccgt gccgtagata ttcgcaccga taacatcgtc gctacgggca ttacctatca
    10681 gttctaacgt cctctcctga ccagcccttc tctgggctgg tttttgattt aagtgctgct
    10741 gtatgactcc ttaagtaaaa atgagcaatt tttgatattt ctggtcattc acctcaatta
    10801 atattattcc ata
//
LOCUS       DAJTNM010000126        10728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTNM010000126 DAJTNM010000000
VERSION     DAJTNM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.126"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            87..1172
                     /locus_tag="OO172_003398"
     CDS             87..1172
                     /locus_tag="OO172_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5513457.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1243..1851
                     /locus_tag="OO172_003399"
     CDS             1243..1851
                     /locus_tag="OO172_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5513458.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1949..3340
                     /gene="selA"
                     /locus_tag="OO172_003400"
     CDS             1949..3340
                     /gene="selA"
                     /locus_tag="OO172_003400"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5513459.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVTHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3337..5181
                     /gene="selB"
                     /locus_tag="OO172_003401"
     CDS             3337..5181
                     /gene="selB"
                     /locus_tag="OO172_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5513460.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5371..6522
                     /gene="yiaY"
                     /locus_tag="OO172_003402"
     CDS             5371..6522
                     /gene="yiaY"
                     /locus_tag="OO172_003402"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5513461.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6687..8225
                     /gene="aldB"
                     /locus_tag="OO172_003403"
                     /pseudo
     CDS             6687..8225
                     /gene="aldB"
                     /locus_tag="OO172_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
     gene            complement(8444..8587)
                     /locus_tag="OO172_003404"
     CDS             complement(8444..8587)
                     /locus_tag="OO172_003404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513462.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            8770..9093
                     /locus_tag="OO172_003405"
     CDS             8770..9093
                     /locus_tag="OO172_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5513463.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9099..10235
                     /locus_tag="OO172_003406"
     CDS             9099..10235
                     /locus_tag="OO172_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5513464.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10232..>10728)
                     /locus_tag="OO172_003407"
     CDS             complement(10232..>10728)
                     /locus_tag="OO172_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5513465.1"
                     /translation="SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMM
                     IDDANIWRFNVAGSKEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQD
                     GTLVPCFQHPYEWWLDLVAIFRKREYLPWRVQYVLDEMLREIRHQLAQSQQLRPEQAA
                     ESED"
BASE COUNT         2550 a         2638 c         2960 g         2580 t
ORIGIN      
        1 gcactttctg accgaatttc ccggctggag catcaactcg ccgccgagaa aaagactgac
       61 tattccacgt tcccggagat ctaattatgg atctattgat tgttttaact tacgtggcgc
      121 tggcgacggc ggcgctggga ggcgtgtttc tggtgagtgg tttgattttg ttgatgaact
      181 acaaccaccc ttacactttt accgcgcaaa aggcagtgat agcgatccct atcacgccac
      241 aggtgacggg aattgttact gaagtcactg acaagaataa tcagcttatt caaaagggcg
      301 aggtgctttt taagctcgac ccggttcgtt accaggcgcg agttgacaga cttcaggctg
      361 acctgatgac ggcgacgcat aatataaaga cgctgcgtgc gcagctcact gaagcgcagg
      421 ccaacaccac ccaggtttca gcggagcgcg accgtctgtt taaaaattat caacgttacc
      481 tgaaaggcag ccaggcggcg gtgaatccgt tctcggaacg tgacatcgac gatgcgcggc
      541 aaaatttcct cgcgcaggat gcgttagtga aaggctcggt ggcggagcag gcgcagatcc
      601 agagtcagct cgacagtatg gtcaacggcg agcaatcgca gattgtgagc ttaagagcgc
      661 aacttactga agcaaaatat aatcttgagc agactgtcat tcgcgcacca agcaatggct
      721 acgtcactca ggtactgatc cgcccaggta catacgcagc tgccttgccg ctgcgtccgg
      781 tgatggtctt catccccgag caaaaacggc aaattgtcgc ccaatttcgg caaaactcgc
      841 tgttacgtct gaaacctggc gatgatgcgg aagtggtgtt taacgcgcta cctgggcagg
      901 tgttccacgg caaattgact agtattttac ctgtcgtgcc aggcggttct tatcaggcgc
      961 agggggtatt gcaatcatta acggtcgttc ccggcacgga cggtgtgctg ggaaccattg
     1021 aactggaccc taacgatgat atcgatgcct tacccgacgg catctacgcc caggtggcgg
     1081 tttactccga ccatttcagc catgtttcgg tgatgcggaa agtgctgcta agaatgacca
     1141 gttggatgca ttatctttat ttggatcatt gagacttata tcacctgtgt tttgcgtgag
     1201 cctgagccat actgatattt ttcctcgcac aaaggactcg gcatgaaact cgtcggtagc
     1261 tacaccagcc cgtttgtacg caaactttct attctgttgt tagaaaaggg cataactttc
     1321 gaatttatta atgaactgcc ctataacgcg gataacggcg tggcgcaatt taacccgtta
     1381 gggaaagtgc cggtgctggt gaccgaagag ggcgaatgct ggtttgattc gccgatcatc
     1441 gctgaatata ttgagttaat gaatgtcgct ccggcgatgt tgccgcgcga tccgctggag
     1501 tcgttgcggg tgcgcaaaat tgaggcgctg gcggatggca ttatggatgc cgggctggta
     1561 tcggtgcgtg aacaggcgcg tccggcggcg cagcagtctg aagatgaatt gttacgccag
     1621 cgggagaaaa tcaaccgcag tctggatgtg ctggaaggat atctggtcga tggcacactc
     1681 aaaaccgata cggtcaatct ggcgactatc gccattgcct gtgctgtcgg atatctcaat
     1741 ttccgccgcg ttgcgccagg ttggtgtgtc gaccgcccac acttagtcaa actggtcgaa
     1801 aacctgttta gccgcgaaag ttttgctcgc accgaaccgc caaaggcttg atgcgcgata
     1861 tgtcctcctg acccatctca cgttacaatc cgtggttatg ttaaacgccc ttctccgtgt
     1921 gagagggcct tgatcagcca ggtttcctat gacaaccgaa acgcgttccc tctatagtca
     1981 acttccggct attgatcgct tattgcgcga tagctccttc ctttctttgc gtgatactta
     2041 tggtcacacc cgcgtggtgg aattgttgcg tcagatgctc gacgaagcgc gagaagtgat
     2101 tcgtggcagc cagacgctgc ctgcgtggtg tgaaaactgg gcgcaagaag tcgatgcccg
     2161 gttgacgaaa gaggcgcaga gcgcgctgcg tccggtgatc aacctgacgg gaaccgtgct
     2221 gcataccaac cttgggcgag ctttacaggc ggaagccgcg gtggaagccg ttgcgcaggc
     2281 tatgcgttcg ccagtgaccc tcgagtacga tctggacgac gccggacgcg gacatcgcga
     2341 tcgggcgctg gcgcagctgc tgtgccgtat tacgggggcg gaagatgcct gtatcgtcaa
     2401 taacaatgcg gcggcggtgt tattgatgtt ggcggccact gccagcggga aagaggtggt
     2461 ggtatctcgc ggcgaactgg tggagattgg cggcgcgttt cgtattcccg atgttatgcg
     2521 tcaggcaggc tgcaccctac acgaagtagg gaccaccaac cgcacgcacg cgaatgatta
     2581 tcgtcaggcg gtgaatgaaa ataccgcact gttgatgaaa gtacatacca gtaactacag
     2641 cattcagggg ttcaccaaag cgatagacga agctgaactg gtggcgctcg gcaaagagct
     2701 ggatgttcca gtggtgactg atttaggcag tggctcgctg gtcgatctta gccagtacgg
     2761 tttgccgaaa gagccaatgc cgcaggagtt gattgcggcg ggcgtcagtc tggtgagctt
     2821 ctccggcgac aagttgttag gcgggccgca ggcagggatt atcgttggta aaaaagagat
     2881 gatcgcccgc ctgcaaagcc acccgctgaa gcgtgcatta cgcgcggata aaatgaccct
     2941 cgcggcgctg gaagccacgt tgcgtcttta tttacaccct gaagctctga gtgaaaaatt
     3001 accgaccctg cgcctgctta cccgcagcgc agaggtcatt caaatccagg cacaacgttt
     3061 acaggctccc cttgtcacac attacggcgc ggagtttgcg gtacaggtta tgccatgtct
     3121 ttcgcagatt ggcagtggtt cgctgccggt tgatcgcctg ccgagcgcgg cattaacgtt
     3181 tacaccccat gatggacgcg gtagccacct tgagtcatta gccgcccgct ggcgtgaatt
     3241 gccagtgccg gtgattggtc gtatttatga cggacgattg tggctggatt tacgctgcct
     3301 tgaagatgag caacggtttt tggagatgtt gttgaaatga ttattgcgac tgccggacac
     3361 gttgaccacg gcaaaacaac cttattgcag gcgattactg gcgtaaatgc tgaccgtctg
     3421 ccggaagaaa aaaagcgcgg catgaccatc gatctcggct atgcctactg gccgcagccg
     3481 gatggtcgcg tgcctggttt tatcgacgtt cccggtcatg aaaagtttct ttccaacatg
     3541 ctggcgggcg ttggtggtat cgatcacgcg ctgttggtgg tggcgtgcga tgacggcgtg
     3601 atggcacaga cccgtgagca tctggcgatt ttgcagctga ccggtaaccc gatgctgaca
     3661 gtggcgctga ccaaagccga tcgtgtggac gaagcgcgtg ttgatgaggt tgaacgccag
     3721 gtaaaggagg ttctgcggga atacggtttt gctgaggcaa aactgtttat caccgcagca
     3781 accgaaggtc ggggaattga tgccctgcgc gagcatctgc ttcagttgcc ggaacgcgag
     3841 cacgccagcc aacatagttt ccgcctcgcg attgaccgcg catttaccgt aaaaggtgcc
     3901 gggctggtcg tcaccggtac ggcgttaagc ggggaagtga aggtaggcga ttcactctgg
     3961 ctgactggtg taaataaacc gatgcgtgta cgtgcgctgc atgcgcaaaa ccagccaaca
     4021 gaaaccgcca atgccgggca gcgtatcgcg cttgacatcg cgggtgatgc ggaaaaagag
     4081 cagattaacc gtggcgactg gctgcttgcc gatgtgccgc cagagccgtt cacacgggtg
     4141 attgtcgagc ttcaaaccca tgcaccgctg acccagtggc agccgctgca tattcaccac
     4201 gccgccagcc acgtcacggg acgcgtttca ctgctggaag ataaccttgc tgaactggtc
     4261 ttcgacaccc cgttatggct ggcagataac gaccgcctgg tattgcgcga tatctctgcc
     4321 cgcaacacgc tggccggagc gcgcgtcgtg atgcttaacc cgccgcgtcg cggtaaacgt
     4381 aaaccagagt atctgcaatg gctggcttcc ctcgctaggg cacagaatga tagcgaagcg
     4441 ttagccgttc acctgcaacg cggcgcggtt aatcttcctg atttcgcctg ggcgcgccag
     4501 ctcaacggcg aagggatgcg cgaactgctg caacagcctg gctatattca ggctggttat
     4561 agcttgttga atgcgccggt tgccgctcgc tggcaacgga aaattctcga cacattagcg
     4621 acttatcatg agcaacatcg cgatgaacct gggcctgggc gcgaacgtct gcgacgtatg
     4681 gcgttgccaa tggaagatga agcgctagta ctgttgctga ttgaaaagat gcgcgaaagc
     4741 ggcgacatcc tcagtcatca cggttggctg catctgccgg atcacaaagc gggcttcagc
     4801 gaagagcagc aggccatctg gcaaaaagca gagccgctgt ttggtgacga gccgtggtgg
     4861 gtgcgtgatc tggcaaaaga gacgggtacc gacgagcagg caatgcgcct gacgctacgc
     4921 caggcggcgc agcaaggaat aattaccgcg atcgtcaaag atcgttatta ccgtaacgat
     4981 cggattgtcg aatttgccaa tatgatccgc gatctcgatc aggagtgtgg ttcaacctgc
     5041 gcggcggatt tccgcgatcg cttaggcgta ggccgaaagc tggcaattca gattctggaa
     5101 tattttgacc gcattggctt tacgcgtcgt cgtggaaatg atcatttatt gcgcgacgca
     5161 ttattatttc cggaaagata aggaaatgat taattaattt ttaaaataaa ttaatacaaa
     5221 tttcttatga ttttaaataa agcacattgt ttaatgaata caatgtgctt tttattagat
     5281 taattttgat aattaataat aatgatcatg ttttcgaatg aaaatcctca gtaagctgcc
     5341 cacccttttt tacactttca ggagtgtgtt atggcatctt caactttctt tattccttct
     5401 gtgaatgtca tcggcgctga ttcattgact gatgcaatga atatgatggc agattatgga
     5461 tttacccgta ccttaattgt cactgacaat atgttaacga aattaggtat ggcgggtgat
     5521 gtgcaaaaag cactggaaga acgcaatatt tttagcgtta tttatgatgg cacccaacct
     5581 aaccccacca cggaaaacgt cgccgcaggt ttgaaattac ttaaagaaaa taattgcgat
     5641 agcgtgatct ccttaggcgg tggttctcca cacgactgcg caaaaggtat tgcgctggtg
     5701 gcagccaatg gcggcgatat tcgcgattac gaaggcgttg accgctctgc aaaaccgcag
     5761 ctgccgatga tcgccatcaa taccacggcg ggtacggcct ctgaaatgac ccgtttctgc
     5821 atcatcactg acgaagcgcg tcataacaaa atggcgattg ttgataagca cgtgactccg
     5881 ctgctttctg tcaatgactc ctctctgatg attggtatgc cgaagtcact gaccgccgca
     5941 acgggtatgg atgccttaac gcacgctatc gaagcgtatg tttctattgc cgccacgccg
     6001 atcactgacg cttgtgcact gaaagccgtg accatgattg ccgaaaacct gccgttagcc
     6061 gttgaagatg gcagtaatgc gaaagcgcgt gaagcaatgg cttatgccca gttccttgcc
     6121 ggtatggcgt tcaataatgc ttctctgggt tatgttcatg cgatggcgca ccagctgggc
     6181 ggtttctaca acctgccaca cggtgtatgt aacgccgttc tgctgccgca tgttcaggta
     6241 ttcaacagca aagtcgccgc tgcacgtctg cgtgactgtg ccgctgcaat gggcgtgaac
     6301 gtgacaggta aaaacgacgc ggaaggtgct gaagcctgca ttaacgccat ccgtgaactg
     6361 gcgaagaaag tggatatccc ggcaggccta cgcgacctga acgtgaaaga agaagatttc
     6421 gcggttctgg cgactaatgc cctgaaagat gcctgtggct ttactaaccc gatccaggca
     6481 actcacgaag aaattgtggt gatttatcgc gccgcaatgt aatcatcatt ccaacaacgg
     6541 ctggcaaatt gtcagccgct ttttcaacta tctctgtaat ccttgcccgt aaattcgtga
     6601 tagctgtcgt aaagctgtta ccgactggcg aagatttcgc cagtcacgtc tacccttgtt
     6661 atacctcaca ccgcaaggag acgatcatga ccaataatcc cccttcagca cagattaagc
     6721 ccggcgagta tggtttcccc atcaagttaa aagcccgcta cgacaacttt attggcggtg
     6781 aatgggtagc ccctgccgat ggtgagtatt accagaacct gacgccggtg accgggcagc
     6841 tgctgtgcga agtggcgtct tcgggcaaac gagacatcga tctggcgctg gatgctgcgc
     6901 acaaagtgaa agataaatgg gcgcacacct cggtgcagga tcgcgcggcg attctgttta
     6961 agattgccga tcgaatggaa caaaacctcg agctgttagc gacagctgaa acctgggata
     7021 acggcaaacc cattcgcgaa accagtgctg cggatgtacc gctggcgatt gaccatttcc
     7081 gctatttcgc ctcgtgtatt cgggcgcagg aaggtgggat cagtgaagtt gatagcgaaa
     7141 ccgtggccta tcatttccat gaaccgttag gcgtggtggg gcagattatc ccgtggaact
     7201 tcccgctgct gatggcgagc tggaaaatgg ctcccgcgct ggcggcgggc aactgtgtgg
     7261 tgctgaaacc cgcacgtctt accccgcttt ctgtactgct gctaatggaa attgtcggtg
     7321 atttactgcc gccgggcgtg gtgaacgtgg tcaatggcgc aggtggggaa attggcgaat
     7381 atctggcgac ctcgaaacgc atcgccaaag tggcgtttac cggctcaacg gaagtgggcc
     7441 aacaaattat gcaatacgca acgcaaaaca ttattccggt gacgctggag ttgggcggta
     7501 agtcgccaaa tatcttcttt gctgatgtga tggatgaaga agatgccttt ttcgataaag
     7561 cgctggaagg ctttgcactg tttgccttta accagggcga agtttgcacc tgtccgagtc
     7621 gtgccttagt gcaggagtca atctacgaac gctttatgga acgcgccatc cgccgtgtcg
     7681 aaagcattcg cagcggtaac ccgctcgaca gcgtgacgca aatgggcgcg caggtttctc
     7741 acgggcaact ggaaaccatc ctcaactaca ttgatatcgg taaaaaagag ggcgctgacg
     7801 tgctcacagg cgggcggcgc aagctgctgg aaggtgaact gaaagacggc tactacctcg
     7861 aaccgacgat tctgtttggt cagaacaata tgcgggtgtt ctaggaggag atttttggcc
     7921 cggtgctggc ggtgaccacc ttcaaaacga tggaagaagc gctggagctg gcgaacgata
     7981 cgcaatatgg cttgggcgcg ggcgtctgga gccgcaacgg taatctggcc tataagatgg
     8041 ggcgcggcat acaggctggg cgcgtgtgga ccaactgtta tcacgcttac ccggcacatg
     8101 ctgcgtttgg tggctacaaa caatcaggta tcggtcgcga aacccacaag atgatgctgg
     8161 agcattacca gcaaaccaag tgcctgctgg tgagctactc ggataaaccg ttggggccgt
     8221 tctgatataa gaagctggtc gcattgggta ttcattgcct gatgcgacgc ttacgcgtct
     8281 tatcatacct acgggaacct gaccgtaggc cggataaggc gtacacgccg catccggcat
     8341 aaacaaagcg cacgttgtta caatttacgt tttaaaccta cgtgcgcaat aacccagact
     8401 acgccaacag aagtgcgttt ctggggcgtg ttttgtcagg agtttaatct cgcgtttcga
     8461 tggtgagcgg gcgaaccagg ccgtaaatca ccattggtga tccgaggcca atcagcaata
     8521 aacggtagat aattgaaggt ttaatacgct taatctgtac tgtggatgtt ttactggagt
     8581 tattcattag tttggccaat agttccacta tttcaatatg ataaacctcg tcctgttgaa
     8641 aaaccagaca gcacatttca gaaagtatat aaaaaacaat atgtcgttat ataaataagg
     8701 aaattgcact gcatcatggc atttcataat atgcgttaat tgttcttttt tatattcttt
     8761 gagggaaata tgttcctgga ctattttgca ctgggagtgc ttatttttgt atttctggtg
     8821 atattctatg ggattattat tttacatgat attccctacc tgatttctaa aaaacgtaat
     8881 catcctcatg ccgacgccat tcatgttgct ggttgggtga gtctttttac gctgcatgtt
     8941 atctggccat ttttgtggat ttgggccacg ctttaccgcc cggagcgggg atggggaatg
     9001 caaatccatg actcctccgt tgtgcaacta caacagcgta ttgccgggct ggagaaacag
     9061 ctcgccgaca tcaaatcctc ttctgccgag taatacttat ggatttactg attattttga
     9121 cctatgtggc ttttgcatgg gcaatgttta agatcttcaa aattcctgta aataaatgga
     9181 ccatttccac agcggccctg ggtggcatat ttattgtcag tggtttaatt ctgttaatga
     9241 actataacca tccgtatacc ttcaaggcgc aaaaagcggt tatttctatt cctgttgtcc
     9301 cacaggtgac aggcgtggtg atcgaagtga cggataagaa aaatacgctg attaaaaaag
     9361 gtgaggtgct atttcgactg gacccgacgc gttatcaggc gcgggtggat cggctgatgg
     9421 cggatatcgt taccgcagaa cataaacagc gggcgttggg cgcagagtta gatgagatgg
     9481 cggcgaatac tcagcaggca aaggccacgc gggataaatt cgctaaagag tatcagcgtt
     9541 acgcacgcgg cagccaggcg aaagtaaacc cgttttcaga acgcgatatt gatgtggcgc
     9601 ggcaaaatta tctggcgcag gaagcctccg taaagtcatc ggcggcggaa caaaaacaga
     9661 tccagagcca gctggatagc ctggtgttgg gtgaacattc tcaaatcgcc agcctgaaag
     9721 cgcagctcgc ggaagcaaaa tataaccttg agcagacgat agtgcgtgcg ccgagcgatg
     9781 gttatgtgac ccaggtgctg attcgtccgg gtacctatgc cgcgtcgctg ccgctacgtc
     9841 cggtgatggt gtttataccc gatcagaaac gacaaattgt ggcgcagttc cgtcagaact
     9901 ccttgctgcg actggcccct ggcgacgatg cggaagtggt gtttaatgct ctgccaggta
     9961 aggtattcag cggcaagctg gcagccatta gtccagctgt tcccggcgga gcttatcagt
    10021 cgaccggcac cttacagacg ttaaacacag cgccgggttc agatggcgtt atcgcgacca
    10081 ttgaactgga tgagcacact gatttgagcg ctttaccaga cggtatttac gcccaggtgg
    10141 cggtctactc tgatcatttc agccatgtct cggtgatgcg caaagtgcta ttacgcatga
    10201 ccagctgggt gcattacctt tatctcgatc attaatcttc gctttccgcc gcttgttcag
    10261 gccgcagttg ctgcgactga gcaagctggt ggcgaatttc acgcagcatt tcatccagca
    10321 catactggac ccgccagggc aggtattccc gtttacggaa gatagccacc agatccagcc
    10381 accattcata cgggtgctga aagcagggca ccagcgtgcc gtcttgcaga tcgctctgca
    10441 cacttttgtc tggcgcaaac acaatcccca ggtgatttcg tgccagctcc agcgccgatt
    10501 gcgtgttatc gcaaacgtaa tttcccttca cgcgataatc ccgcacctct ttactcccgg
    10561 cgacgttgaa gcgccagata ttcgcgtcgt cgatcatcat cgagtcgatc aaaatacagg
    10621 agtgatgttc gagttcgtca ggacggctaa taggatgttt ctcaagatag cgctggctgg
    10681 cataagcggt tacggcatat tgtgtaataa aactggcaac cagcgatt
//
LOCUS       DAJTNM010000127        10464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTNM010000127 DAJTNM010000000
VERSION     DAJTNM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.127"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(67..1071)
                     /gene="cra"
                     /locus_tag="OO172_003408"
     CDS             complement(67..1071)
                     /gene="cra"
                     /locus_tag="OO172_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5513466.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAEMVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1153..1239)
                     /locus_tag="OO172_003409"
     CDS             complement(1153..1239)
                     /locus_tag="OO172_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5513467.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1251..1742)
                     /gene="ilvN"
                     /locus_tag="OO172_003410"
     CDS             complement(1251..1742)
                     /gene="ilvN"
                     /locus_tag="OO172_003410"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5513468.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1745..3469)
                     /gene="ilvI"
                     /locus_tag="OO172_003411"
     CDS             complement(1745..3469)
                     /gene="ilvI"
                     /locus_tag="OO172_003411"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5513469.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3588..3632
                     /locus_tag="OO172_003412"
     CDS             3588..3632
                     /locus_tag="OO172_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513470.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3787..4731)
                     /gene="leuO"
                     /locus_tag="OO172_003413"
     CDS             complement(3787..4731)
                     /gene="leuO"
                     /locus_tag="OO172_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5513471.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     EMEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGCDKGHQWMEEQLVSICKR"
     gene            5391..5477
                     /gene="leuL"
                     /locus_tag="OO172_003414"
     CDS             5391..5477
                     /gene="leuL"
                     /locus_tag="OO172_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5513472.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5570..7141
                     /gene="leuA"
                     /locus_tag="OO172_003415"
     CDS             5570..7141
                     /gene="leuA"
                     /locus_tag="OO172_003415"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5513473.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7141..8232
                     /gene="leuB"
                     /locus_tag="OO172_003416"
     CDS             7141..8232
                     /gene="leuB"
                     /locus_tag="OO172_003416"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5513474.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8235..9635
                     /gene="leuC"
                     /locus_tag="OO172_003417"
     CDS             8235..9635
                     /gene="leuC"
                     /locus_tag="OO172_003417"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5513475.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9646..10251
                     /gene="leuD"
                     /locus_tag="OO172_003418"
     CDS             9646..10251
                     /gene="leuD"
                     /locus_tag="OO172_003418"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5513476.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10300..>10464)
                     /locus_tag="OO172_003419"
     CDS             complement(10300..>10464)
                     /locus_tag="OO172_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5513477.1"
                     /translation="AATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVA
                     LFLTAKVKDV"
BASE COUNT         2736 a         2745 c         2562 g         2421 t
ORIGIN      
        1 tgatgtccag tcccgtactc tacgcgccag agggaaattc acctggcgcg tatttttgtt
       61 cgcggcttag ctacggctga gcacgccgcg gcgatagaga ttacgtttaa tgcgcgttaa
      121 accaggtttt ggcttacgcg gttcgtccag gcttgccagg acaatctcca gcacacgctc
      181 tgcgacatcg cggtgacgtt gagccactgc cagcaccgga cactgtaaga agtcgagcag
      241 ttcgttatcg ccaaaggtgg caattgccag gtcagaaggc agtttgccgt cgcgacgcag
      301 cgtgacatcc atcactcctt gcaacaacgc aaacgacgtt gtgaacagcg cctgcggcat
      361 cggatgcgtt tccagccatt tttcgaataa ctgggcagcc gcctcccgct catagctgtt
      421 ggcatacagg aaatgcactt cgcgcggatc atctttccaa gcagtacgga aaccttgttc
      481 acgcaggaag ctgacagaaa gctccggcag cgcgccaaga taaagcacca tctcggcggg
      541 aaacttacgt aactcttccg ccagcatttc ggcatcatcc tgatcggcac caaccacgct
      601 ggtgaaatgt tcacgatcga gggcgcggtc cagcgcgaca atcgggaacg ggtcgttagc
      661 ccagcgttga taaaaaggat gctcaggtgg caacgacgtc gaaacaataa tggcatcaac
      721 ctgacgctgt aaaaggtgct caatgcagcg catttcgttg tctggctgat cttctgagca
      781 ggcaatcagc agttgataac cccgttgccg cgcctggcgt tcaagatagt tagcgatgcg
      841 ggtatagctg gtgttctcca gatcggggat cacaagacca atagaacgtg tgcgtccagc
      901 acgaagccca gctgccacgg cgttcgggtg gtaattgtgc tcacgcacca cagccatgac
      961 tttttcaacg gttttgtcgc tcacacggta ttgcttcgct ttgccgttaa taacatagct
     1021 tgcagtggtc cgcgacactc ccgccagccg agcgatttca tccagtttca caattgcccc
     1081 ttgcgtaaaa tgtaaaaacc atagccattg tactggtatg ggttaaattc attaacatct
     1141 aagcgcagaa aattaactgc ggcaactgct tttattccag gttcccactg atttcgcaaa
     1201 aaaagcccaa cgtgacatgt tgggctgcaa attgcgcact gagatcattc tcaacgcatt
     1261 attttatcgc cgcgcgaaag tccgaccaca ccagagcgag caacttccac aattttcgcc
     1321 acatcgcgaa tcgatgctaa aaatgcatca agcttatcgc tggtgcctgc taattgaacg
     1381 gtataaagcg agggggtgac atcgataatt tgcccacgga atatttccgt attacgtttc
     1441 acttcgtcac gcccgtaacc gctggcctga attttcacca gcatgatttc ccgctcaaca
     1501 tgcgcgccct gccccaactc actcacgcgc aagacatcga ccagcttgtg taattgcttt
     1561 tcgatctgct caagtacttt ttcatcgccc acggtctgga tggtcatacg cgataatgtc
     1621 ggatcgtcgg ttggcgcaac ggtcaggctt tcaatgttgt agcctcgctg ggaaaaaagg
     1681 ccaatcacgc gggataacgc gcctgattca ttttcgagta agactgataa tatccggcgc
     1741 ataatcaggt tctctccgtt ttgcttaacc acatttcatc cattccgccc ccgcgaatct
     1801 gcatcgggta gacgtgctca ctgccatcga cggtaacatc aacaaacacc aggcgattat
     1861 tgcgcacctg ttccagcgcc tcgctaagtt tgctttccag ctcatgcgga tgagagatct
     1921 ggatcccgac gtgaccatag gcttccgcca gacggacgaa atcgggtagt gattgcatat
     1981 aagattgtga atgacggccg gaatagatca tgtcctgcca ctgtttcacc atccccagat
     2041 agcggttatt gagattcacc accagtacgg gcaactcgta ttgcaacgcg gtagacagtt
     2101 cctggatgtt catctgaata ctgccgtcgc cagtgacgca aaccacggtt tcttctggca
     2161 acgccatttt gacgcccagt gccgcaggta aaccaaaacc catcgtgccg agaccgccgg
     2221 aattgatcca gcgacgcggt ttgtcgaatg gataataaag cgcagcaaac atctggtgct
     2281 gcccgacatc ggacgtcacg taagcgtccc cattagtcaa ccgccaaaga gtctcgatca
     2341 ccgcctgcgg tttaatcttt tcactgtgag tgtcatattt caggcactga agagcgcgcc
     2401 actgttcaat ctgctgccac cagtcgcgga tctcatccag cggttgatgg gcggattctt
     2461 gcgacaggag ttcaagcatt tgttcgagga cctggcgagc atcccccaca atcgggatat
     2521 ccgcagtcac ggttttagaa atggaagtag gatcaatatc gatatgcaga acagtggcat
     2581 ttgggcagta ctttgccaga ttgttcgtcg ttcggtcatc aaatcgtacc ccgacggcga
     2641 aaatcacatc cgcgttatgc atcgtcatat tggcttcgta ggtaccgtgc attcccagca
     2701 tgcccagtgc ctgacgatgc gttgccggaa acgcccccag ccccatcaat gaggaaacaa
     2761 cgggcagatt caacgcctcc accgtttctt tcaactgctg atggcagccc gccatgattg
     2821 ccccaccgcc tacgtagaca accggttttt ttgccgctac cagcgtttgc agagcacgct
     2881 taatttgccc tttatgtccg gtagtagtgg gattgtaaga acgcatactg accgactcag
     2941 ggcagacata gggtaatttg ttcgccggat taagaatatc tttcggtaaa tcaacgacca
     3001 ctggtcctgg gcgaccactt gccgccagcc agaaagcctt tttcagcacc tgcggaatgt
     3061 cttccgtttg cttaaccaga aaactgtgtt taaccaccgg tcgcgaaatc cccaccatgt
     3121 cgcactcctg aaaagcatcg taacctatca acgaggtcgc tacctgcccg gaaaggacaa
     3181 ctaatggaat ggaatccata taagcggtgg cgatgccggt aatcgcattg gtcgcccctg
     3241 gacccgacgt taccagcacg acgccgactt ccccggtcgc acgcgccagg ccatcggcca
     3301 tatgcaccgc cgcctgctca tgacgaacta atacatgatc aataccaccc acggtatgca
     3361 atgcatcgta aatatcaagg actgcgcctc cgggataacc gaatacttgt ttaacgccct
     3421 gatcgataag cgatcggacg accatctcgg ctccagacaa catctccatg gcctgcctca
     3481 ctgtttgacg gaaaaaatgt gtaaaacaca ttaaccgtcc gatcttgctt aagcaattgg
     3541 cagccagaga cataaatgca gggtaataag aataaacagg aggaaaggtg aaaagaaacg
     3601 ataaaatcct ccattacgaa ggattaaatt gataataaat cggctgaatc ccacaactta
     3661 cagcaatttc tctatttttt taattaatcc taaaatagag agtccacaca ttcagaataa
     3721 aattctacat tcaataaaaa atgcagaata aaccagacat tcatgtctgg cctattctgc
     3781 attcagttag cgtttgcaaa ttgagactaa ttgttcttcc atccactgat ggcctttatc
     3841 gcacccggca gcttcatgcc aagagagata acaggttctg ctgttttgtt ttaacggcag
     3901 cggtaatacc tgtaattcta aggattcagc gaactcttca gccagccaac gcggcgcaat
     3961 agcgaccaaa tgcgtttgcg acaccacgct aagtacgctc atcattgcca tgccctgata
     4021 cgcgatactg gcttgcttat ctaccgtgtc ataccaaggt tgactaaatg acgcgaaacg
     4081 atcgagcgaa accgccgcat gttgttcgtt ataaacatca tgtttcagta acgggccttt
     4141 aattgttgga tgatttttgc tggctaccag caccatttca tctttaaata atggcacgct
     4201 ggtaaattca ggacgatgga agtcttcata actaatcaca aactccattt cctgataacg
     4261 cagctgatgt tcagtgttct gatttaatga agacttgaac ataacatgta tatttggcgc
     4321 aatctgctca atgtgattat aaatctgcga ggtcagaatg ctgtctaacg ggctgcaaac
     4381 acaaagatga aatacacgtt cactgctcgc gggttcaaaa cctgaaccag gcaattcatt
     4441 ttgtactagt tgcaatgcct ggcgaactga accaaaaagt tgaaatgcgc gagcagtcgg
     4501 ttgaatacca cggccataac gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc
     4561 aacagcgtta ctgaccgcag gttgcgacat acccagaaca tgagcggcac gggtaatgtt
     4621 ttgctcctgc atcacggcat cgaaaacggt taataagttg agatcgacca tgcgtagctg
     4681 tggcttgctt aactccgccg tctctgaatg atctgtttgt acctctggca tacttaactc
     4741 cactgtcaca cttaactccc tttcccttat tggaatgcag agaaataatc ctgaagaata
     4801 tgatttatca tgcatataaa ataagaaaaa gcaaaatgag taaaattcgg aaaacataaa
     4861 gatacgggtt ctgataaaaa cagaatccat tgccgtggaa tgagtcattt accacagcat
     4921 aataatccat aatgtttttg ttgcgaaaac aatctaatca taactaccgc gaatactcaa
     4981 tcatctacaa aatggattaa atgtgcttat ttaataatta ttttatatat ttaatgcatt
     5041 aattcttaac attaattgat caataatatt caccaaatca atatcaaaaa aaatcgcaaa
     5101 acatataatt caatacaaat cattaggata ggttttgcaa cgcgtgcatt ttgtcccctt
     5161 tttcctcgtt gattagatgc aaaaatttat gctgaaatat gtcaaccgat gaaaagcgtc
     5221 ggtagttaag cagaaattaa tatcgcttac tttaaccacc gtagcacaat tagctaattt
     5281 tacggatgca gaactcacgc tggcgggacg tttttattgc gtcagggttg acatccgttt
     5341 ttgtatccag taactctaaa agcatatcgc attcatctgg agctgattta atgactcaca
     5401 tcgttcgctt tatcggtcta ctactactaa acgcatcttc tttgcgcggt agacgagtga
     5461 gcggcatcca gcattaagcc agcacgcagt caaacaaaaa acccgcgcca ttgcgcgggt
     5521 ttttttatgc ccgaagcgag gcgctctaaa agagacaagg acccaaacca tgagccagca
     5581 agtcattatt ttcgatacca cattgcgcga cggtgaacag gcgttacagg caagcttgag
     5641 tgtgaaagaa aaactgcaaa ttgcgctggc ccttgagcgt atgggtgttg acgtgatgga
     5701 agtcggtttc cccgtctctt cgccgggcga ttttgaatcg gtgcaaacca tcgcccgcca
     5761 ggttaaaaac agccgcgtat gtgcgttagc tcgctgcgtg gaaaaagata tcgacgtggc
     5821 ggccgaatcc ctgaaagtcg ccgaagcctt ccgtattcat acctttattg ctacctcccc
     5881 aatgcacatc gccaccaagc tgcgcagcac cctggacgag gtgatcgaac gcgctatcta
     5941 tatggtgaaa cgcgcccgta attacaccga tgatgttgaa ttttcttgcg aagatgccgg
     6001 gcgtacaccc attgccgatc tggcgcgagt ggtcgaagcg gcgattaatg ccggtgccac
     6061 caccatcaac attccggaca ccgtgggcta caccatgccg tttgagttcg ccggaatcat
     6121 cagcggcctg tatgaacgcg tacctaacat cgacaaagcc attatttccg tacataccca
     6181 cgacgattta ggcctggcgg tcggcaactc actggcggcg gtacatgccg gtgcacgcca
     6241 ggtggaaggt gcaatgaacg ggatcggcga gcgtgccggt aactgttcgc tggaagaagt
     6301 catcatggcg attaaagttc gtaaggatat tctcaacgtc cacaccgcca ttaatcacca
     6361 ggagatatgg cgcaccagcc agttagttag ccagatttgt aatatgccga tcccggcaaa
     6421 caaagccatt gttggcagcg gcgcattcgc tcactcctcc ggtatccacc aggatggcgt
     6481 gctgaaaaac cgcgaaaact acgaaatcat gacaccagaa tctattggtc tgaaccaaat
     6541 ccagctgaat ctgacctctc gttcggggcg tgcggcggtg aaacatcgca tggatgagat
     6601 ggggtataaa gaaagtgaat ataatttaga caatttgtac gatgctttcc tgaagctggc
     6661 ggacaaaaaa ggtcaggtgt ttgattacga tctggaggcg ctggccttca tcggtaagca
     6721 gcaagaagag ccggagcatt tccgtctgga ttacttcagc gtgcagtctg gctctaacga
     6781 tatcgccacc gccgccgtca aactggcctg tggcgaagaa gtcaaagcag aagccgccaa
     6841 cggtaacggt ccggtcgatg ccgtctatca ggcaattaac cgcatcactg actataacgt
     6901 cgaactggtg aaatacagcc tgaccgccaa aggtcacggt aaagatgcgc tgggtcaggt
     6961 ggatattgtc gccaactaca acggtcgccg cttccacggc gtcggcctgg ccaccgatat
     7021 tgtcgagtcc tccgccaaag ccatggtgca cgtactgaac aatatctggc gtgccgcaga
     7081 agtcgaaaaa gagttgcaac gcaaagctca acacaacgaa aacaacaagg aaaccgtgtg
     7141 atgtcgaaga attaccatat tgccgtattg ccgggagacg gtattggtcc ggaagtgatg
     7201 acccaggcgc tgaaagtgct gaatgccgtg cgcaaccgct ttgcgatgcg catcaccacc
     7261 agccattacg atgtaggcgg cgcagccatt gataaccacg ggcaaccact gccgcctgcg
     7321 acggttgaag gttgtgagca agccgatgcc gtgctgtttg gctcggtagg cggcccgaag
     7381 tgggaacatt taccaccaga ccagcaacca gaacgcggcg cgctgctgcc gctgcgtaag
     7441 cacttcaaat tattcagcaa cctgcgcccg gcaaaactgt atcaggggct ggaagcattc
     7501 tgtccgctgc gtgcagacat tgccgctaac ggcttcgaca tcctgtgcgt gcgcgaactg
     7561 accggcggca tctatttcgg tcagccaaaa ggccgcgaag gtagcggaca atatgaaaaa
     7621 gcgtttgata ccgaggtgta tcaccgtttt gagatcgagc gtattgcccg catcgcgttt
     7681 gaatctgctc gtaaacgtcg ccacaaagtc acctcaatcg acaaagctaa cgtgctgcaa
     7741 tcctctattt tatggcgtga aatcgtcaac gagatcgcca cggaataccc ggatgtcgaa
     7801 ctggcgcata tgtacatcga caacgccacc atgcagctga ttaaagatcc atcacagttt
     7861 gacgttctgc tgtgctccaa cctgtttggc gacattctgt ctgacgagtg cgcaatgatc
     7921 actggctcga tgggcatgtt gccttccgcc agcctgaacg agcaaggttt tggactgtat
     7981 gaaccggcgg gcggctcggc accagatatc gcaggcaaaa acatcgccaa cccgattgca
     8041 caaatcctgt cgctggcact gctgctgcgt tacagcctgg atgccgatga tgcggcttcc
     8101 gccattgaac gcgccattaa ccgtgcatta gaagaaggca ttcgcaccgg ggatttagcc
     8161 cgtggcgctg ccgccgttag taccgatgaa atgggcgata tcattgcccg ctatgtggca
     8221 gaaggggtgt aatcatggct aaaacgttat acgaaaaatt attcgacgct cacgtagtgt
     8281 acgaagccga aaacgaaacc ccgctgttat atatcgaccg ccacctggtg catgaagtga
     8341 cctcaccgca ggcgttcgat ggtctgcgcg cccacggtcg cccggtacgt cagccgggca
     8401 aaaccttcgc cactatggat cacaacgtct ctacccagac caaagacatt aatgcctgtg
     8461 gtgaaatggc gcgcatccag atgcaggagc tgattaaaaa ctgcaaagaa tttggcgtcg
     8521 agctgtatga cctgaatcac ccgtatcagg ggatcgtcca cgtaatgggg ccggaacagg
     8581 gcgtcacctt gccggggatg accattgtct gcggcgactc gcataccgcc acccacggcg
     8641 cgtttggcgc actggccttt ggtatcggca cttccgaagt tgaacacgta ctggcaacac
     8701 aaaccctgaa acagggccgt gcgaagacca tgaaaattga agtccagggc aaagccgcgc
     8761 cgggcattac cgcaaaagat atcgtgctgg caattatcgg taaaactggc agcgcaggcg
     8821 gcaccgggca tgtggtggag ttttgcggcg aagcaattcg tgatttaagc atggaaggtc
     8881 gtatgaccct gtgcaatatg gcgatcgaaa tgggcgcaaa agccggtctg gttgctcctg
     8941 acgaaaccac ctttaactat gtcaaaggcc gtctgcatgc gccgaaaggc aaagatttcg
     9001 acgacgccgt tgcctactgg aaaaccctgc aaaccgacga aggcgcaact ttcgataccg
     9061 ttgtcactct gcaagcagaa gaaatttcac cgcaggtcac ctggggcacc aatcccggcc
     9121 aggtgatttc cgtgaacgac aatattcccg atccggcttc gtttgccgat ccggttgaac
     9181 gtgcgtcggc agaaaaagcg ctggcctata tggggctgaa accgggtatt ccgctgaccg
     9241 aagtggctat cgacaaagtg tttatcggtt cctgtaccaa ctcgcgcatt gaagatttac
     9301 gcgcggcagc ggagatcgcc aaagggcgaa aagtcgcgcc aggcgtgcag gcactggtgg
     9361 ttcccggctc tggcccggta aaagcccagg cggaagcgga aggtctggat aaaatattta
     9421 ttgaagccgg ttttgaatgg cgcttgcctg gctgctcaat gtgtctggcg atgaacaacg
     9481 accgtctgaa tccgggcgaa cgttgtgcat ccaccagcaa ccgtaacttt gaaggccgcc
     9541 aggggcgcgg cgggcgcacg catctggtca gcccggcaat ggctgccgct gctgctgtga
     9601 ccggacattt cgccgacatt cgcaacatta aataaggagc acaccatggc agagaaattt
     9661 atcaaacaca caggcctggt ggttccgctg gatgccgcca atgtcgatac cgatgcaatc
     9721 atcccgaaac agtttttgca gaaagtgacc cgtacgggtt ttggcgcgca tctgtttaac
     9781 gactggcgtt ttctcgatga aaaaggccaa cagccaaacc cggacttcgt gctgaacttc
     9841 ccgcagtatc agggcgcttc cattttgctg gcacgagaaa acttcggctg tggctcctcg
     9901 cgtgagcacg cgccctgggc attgaccgac tacggtttta aagtggtgat tgcgccgagt
     9961 tttgctgaca tcttctacgg caatagcttt aacaaccagc tgctgccggt gaaattaagc
    10021 gatgcagaag tggacgaact gtttgcgctg gtgaaagcca atccggggat ccatttcgac
    10081 gtggatctgg aagcgcaaga ggtgaaagcg ggagagaaaa cctatcgctt taccatcgat
    10141 gccttccgcc gccactgcat gatgaatggt ctggacagca tcggcctgac cctgcagcac
    10201 gacgacgcga ttgcgtctta tgaagcaaaa caacctgcgt ttatgaatta gaccccttgc
    10261 ccggtcaaat gaccgggctt tccgctatcg tccacgtcat caaacgtctt taacctttgc
    10321 ggttaaaaat aatgcgacaa cagaaataat cgcaattacc cagtagacag caaagtgccc
    10381 ccaactttgt gcaattgctc cctgaataac accagccaga attaccccgg tagagatagt
    10441 gttggtaaat aaggtggtag ctgc
//
LOCUS       DAJTNM010000128        10380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTNM010000128 DAJTNM010000000
VERSION     DAJTNM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.128"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..1280
                     /locus_tag="OO172_003420"
                     /pseudo
     CDS             1..1280
                     /locus_tag="OO172_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
     gene            1332..2487
                     /locus_tag="OO172_003421"
                     /pseudo
     CDS             1332..2487
                     /locus_tag="OO172_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(2453..2575)
                     /locus_tag="OO172_003422"
     CDS             complement(2453..2575)
                     /locus_tag="OO172_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513478.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2778..4464
                     /locus_tag="OO172_003423"
                     /pseudo
     CDS             2778..4464
                     /locus_tag="OO172_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4502..6874
                     /locus_tag="OO172_003424"
     CDS             4502..6874
                     /locus_tag="OO172_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5513479.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNTPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            6931..9714
                     /locus_tag="OO172_003425"
     CDS             6931..9714
                     /locus_tag="OO172_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5513480.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETFESMG
                     LRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     SEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10076..>10380
                     /locus_tag="OO172_003426"
     CDS             10076..>10380
                     /locus_tag="OO172_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5513481.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT         3068 a         2290 c         2241 g         2781 t
ORIGIN      
        1 ccgtgccgca ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga
       61 tgctcaggat ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg
      121 ttattacact gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc
      181 ggaagataaa caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg
      241 gcaacctcaa aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata
      301 ttacaactcc ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag
      361 cgatcagtta accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc
      421 ttttatgctt tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga
      481 tcaatatcag aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt
      541 ttattctgtt gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta
      601 tgacaataca attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc
      661 gctgaacggg gcgcaaaaag gctataagag tcagacctat cctggcggta ctcaccaccc
      721 caatgtttat gtggtggaaa ggaaaacttc aacccggtaa ttatgacaag ctgatttccg
      781 caatggattt ctacccaaca gctcttgatg cagccgatat cagcattcca aaagacctta
      841 agctggatgg tgtttccttg ctgccctggt tgcaagataa gaaacaaggc gagccacata
      901 aaaatctgac ctggataacc tcttattctc actggtttga tgaggaaaat attccattct
      961 gggataatta ccacaaattt gttcgccatc agtcagacga ttacccgcat aaccccaaca
     1021 ctgaggactt aagccaattc tcttatacgg tgagaaataa cgattattcg cttgtctata
     1081 cagtagaaaa caatcagtta ggtctctaca aactgacgga tctacagcaa aaagataacc
     1141 ttgccgccgc caatccgcag gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg
     1201 acagcagcca gccaccgctt agcgaggtaa atcaggagaa gtttaacaat atcaagaaag
     1261 cactaagcga agcgaaataa ctaaaccttc atgcggcgga tttttccgcc gccttattga
     1321 gcgagatagc aatgcacgtt acagccaagc cctccagttt tcaatgtaat ctcaaatgtg
     1381 attactgttt ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg atggatgaca
     1441 gcactctgaa agagttcatc aaacaatata tcgaagcgtc tggcaatcag gtctatttta
     1501 cctggcaagg cggtgaaccc actctggctg gcctggattt tttccgtaaa gttattcact
     1561 atcaacaacg ctatgcaggc caaaaacgta tttttaatgc attacaaacg aatggcattt
     1621 tattgaataa tgaatggtgt gcctttctca aagaacatga ttttctggtt ggtatctcga
     1681 tcgatggccc ccaggagtta catgaccgtt acagacgcag taattcaggt aacggtactt
     1741 ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata tcaagtagag tttaatacgt
     1801 taaccgtcat taataacgtt aatgtccatt accctcttga ggtttatcat tttttaacat
     1861 ctatcggcag taaacatatg caatttatcg aattgctaga aaccgggacg ccgaatattg
     1921 atttcagtgg tcatagtgag aacacattcc gtatcattga tttttctgtg cctcccacgg
     1981 cttatggcaa gtttatgtca accattttta tgcaatgggt taaaaacgat gtgggtgaaa
     2041 ttttcattcg tcagtttgaa agctttgtca gccgtttttt ggggaatggg catacaagtt
     2101 gtattttcca ggagtcctgc aaggataatc tggttgttga aagtaatgga gacatttacg
     2161 aatgcgacca ttttgtctat ccacagtaca aaattggaaa cattaataaa tctgaactca
     2221 aaacgatgaa cagtgtacaa ctgacagcgc aaaaaacgga tttcagcgaa atgtcagcaa
     2281 tgtgcatata aacctatctg caatggcggt tgtcctaagc atcgtattac taaagtaaac
     2341 aatgagactg tttcttattt ttgcgaaggt tataaaatcc ttttttcaac catggtacct
     2401 tatatgaacg ccatggttga gttagctaag aacagagtac cgctttacca cattatggat
     2461 gttgcaaaac aaatggagaa taattaataa cgctctcttt taactccatt cttagcctcc
     2521 ttcttatgac cttaacaagg cacccgacat aaattcctgt cgggtgtttc gtcatcatca
     2581 tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca gggcattagc aatacccttg
     2641 attatcaata gcataaccat aaccactgaa aattaaaaat ttctatatat ttcatattgt
     2701 taaatgatac ttaaataaat ttcatcagta aatcattgcc atagataatc attggatggc
     2761 acaatcaatt ttcgctaatg ataaccattc ccattacgtt ttgtatgttg atagcaaagt
     2821 atctctgtct gctgaagccc ttttggctgc gtaagaataa taaaacttca gtgttgttaa
     2881 tcattattat cctcgcaatg atccttggcg tggttaaaat tcaggtctgg ttaaacgact
     2941 ggaataatga ttttttcaat gcgctaagcc agaaagaaac cgataagctt tggcagcttg
     3001 ttctttggtt ccctgccctg ctggggatct ttgtattaat atccgtaaac aaaacatggt
     3061 taattaaact actgactatt cgctggcgcg aatggttaac agattactac cttaatcgct
     3121 ggtttgcaga taaaaactac tacttcacgc agatctacgg cgagcataaa aatacagata
     3181 accctgacca acgtatcgct gaagacattc tcttactgat caacaaaacg ctcagtcttt
     3241 catttggctt catccagtca cttagcatgc tgatcacctt taccgttatt ctttggcaaa
     3301 gcgcgggtac gctctcattc actgttggtg gaacagaatg gaatatccag gggtatatgg
     3361 tctataccgt cgtgctcatt gtgatcggtg gaactttatt tacccataag gtgggtaaac
     3421 gtattcgtcc gcttaatgtg gaaaaacaac gtagcgaagc gacatttcga actaatcttg
     3481 tgcagcataa taagcaggca gaactgattg cgctaagcaa tgctgagtct ctacaacgcc
     3541 aggagttgcg cgacaatttt cataccatta aagagaactg gcatcgttta atgaatcgtc
     3601 agcggtggct tgattactgg cgaaaatatt tattcgcgct cgcttagcgt tcttccctac
     3661 tttctgttat tgccgcagtt tattagcggg cagatcaatc tgggcggact gatgaaatcg
     3721 cgccaggcat ttatgctagt atcgaacaat ttaagctggt ttatttataa atatgacgaa
     3781 cttgctgaac tggctgcggt tatcgatcgc ttgtatgagt tccatcaact cactgaatag
     3841 cgccctacga ataagcctaa aaattgccaa catgcggtac aagtggctaa tgcgagtatt
     3901 cgtacgcctg ataataagat catattagag aacttgaact ttcatgtttc gccaggcaaa
     3961 tggctattac tgaaaggcta ctctggtgcg ggaaaaacca cactgcttaa aacattatcc
     4021 cactgctggc cgttatttaa aggtgatatt tcttctcctg ctgacagttg gtatgtgtca
     4081 caaacaccgt taatcaaaag cggcttactg aaagagatta tttgtaaagc acttcccctg
     4141 cccgtagacg ataaatcgtt gagcgaagta ctgcatcagg ttggtctggg gaaattggct
     4201 gcgcgtattc atgaccacga tcgctgggga gatattcttt ccagcggcga aaaacaacgt
     4261 atcgccctgg cacgattaat tttacgacgt ccgaaatgga tatttcttga cgaaactacc
     4321 tctcatcttg aggaacaaga ggctatacgc ttactgcgtt tagtgcgtga aaaactaccc
     4381 acaagcggcg tcattatggt tacacatcaa cccggtgtct ggaacctggc tgatgatatt
     4441 tgtgacatta gcacggtttt ataacaacaa taaaagccct gctgtacgga gatataaata
     4501 aatgaagcga gttcttattc ctggcgtcat tttatgtggc gctgatgtgg cgcaggccgt
     4561 cgatgacaaa aacatgtaca tgcatttttt tgaagagatg acggtctatg ctcctgtccc
     4621 tgtacccgta aacggcaaca cgcattacac cagtgaaagc atcgagcgtt taccgaccgg
     4681 gaatggcaat atcagcgatc tgctgagaac caaccctgcg gtacgcatgg attcaacgca
     4741 aagtacctcg ttgaaccagg gagatattcg ccctgagaaa atctctattc acggtgcgtc
     4801 gccctaccag aatgcctatt tgattgacgg tattagtgca actaataacc tgaacccagc
     4861 gaatgagtcc gatgccagta gtgcaaccaa tattagcggg atgtcacagg ggtattatct
     4921 tgatgtcagc ttactggaca atgtgacgct ttatgacagt tttgtgccgg ttgaatttgg
     4981 tcgcttcaat ggcggggtaa ttgatgcaaa gatcaaacgc ttcaacgctg atgatagcaa
     5041 ggtgaaattg ggttatcgca ctacgcgttt ggactggtta acatcgcata tcgatgagaa
     5101 taacaagagc gcatttaatc aaggttcttc aggaagtacc tatttctctc cagattttaa
     5161 aaagaacttt tataccttgt cgtttaatca ggagctggct gataactttg gcgttaccgc
     5221 cggtttatcg cgccgccagt ctgatatcac ccgcgcggat tatgtttcga atgacggcat
     5281 tgtcgccggt cgggcacagt ataaaaacgt tatcgatact gcattgagca aatttacctg
     5341 gtttgccagc gaccgcttta cccacgattt aaccttaaaa tataccggct ccagccgtga
     5401 ttataatacc agcaccttcc cgcaatctga tcgcgaaatg ggtaataaat cctatggtct
     5461 ggcatgggat atggatacgc aactcgcatg ggccaaacta cgtaccaccg ttggttggga
     5521 tcatattagt gattataccc gtcacgatca tgacatctgg tacaccgaac tttcatgtac
     5581 atatggtgat attacagggc gttgtacccg tggcggatta ggacacattt cccaggctgt
     5641 agataattac accttcaaaa cacgcctgga ctggcaaaaa ttcgccgtgg gtgatgtttc
     5701 gcatcaaccc tacttcggcg cggaatacat ctattccgat gcatggactg aacgccataa
     5761 ccagtctgaa tcctatgtga ttaatgctgc aggaaagaaa actaaccata ccatttacca
     5821 taaaggtaaa ggcagcctgg gaattgacaa ctacacgctg tatatggcgg atcacattag
     5881 ctggcggaat gtgtcgttaa tgcccggtgt gcgttatgac tatgacaact atctgtcaaa
     5941 ccacaatatc tccccgcgct ttatgacgga atgggatatt tttgctgatc aaacctcaat
     6001 gattaccgcc ggttataacc gttactatgg cgggaatatt cttgatatgg gattacgtga
     6061 tatccgcaat agctggacgg aatcggtatc aggtaataaa accctgacgc gttatcagaa
     6121 tttgaaaacg ccttataacg atgaactggc aatgggattg cagcaaaaaa tcgataagaa
     6181 cgttattgca cgcgcaaact atgtttaccg tgaagcgcat gatcaaatca gcaaaagcag
     6241 tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg gcaaaaccaa
     6301 aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc gccaggtaga
     6361 tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt cgttaaacaa
     6421 tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta atctggtctc
     6481 ttacgatagc gttccagtgg cagattttaa taccccatta aagatctcct taaacatgga
     6541 tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc aagaagcgcg
     6601 taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat attcagatta
     6661 caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc gcttgtcctg
     6721 gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc tcaatgtact
     6781 ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg ccagtggccg
     6841 tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact taaccgggta
     6901 aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt actgacgtta
     6961 gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttacagca ggatgaaaag
     7021 ttaattaccg ggcaactgga caatggcttg cgatatatga tttatccgca tgctcatcca
     7081 aaggatcagg taaatttatg gctgcaaatt cataccggtt cattgcagga agaagacaat
     7141 gagcgcggcg tggctcattt tgtagaacat atgatgttta acggcgcaaa aacatggccg
     7201 ggtaataaag tcatcgaaac atttgagtca atgggcctgc gttttggtct cgatgttaat
     7261 gcctatacca gctatgacga aacggtgtat caggtgagtt tgccgactac gcagaaacaa
     7321 aatctgcaac aagtgatggc aatcttcagt gaatggagta atgccgcaac ctttgaaaaa
     7381 ctcgaagtag acgctgaacg tggcgtaatt tctgaggaat ggcgtgccca tcaggatgcg
     7441 aaatggcgca cctctcaggc gcgccgccct ttcctgctgg ctaatacccg taatttagac
     7501 cgtgaaccta tcggcctgat ggatactgtc gccacggtca caccggcaca attgcgccaa
     7561 ttttatcaac gctggtatca accaaataat atgaccttta tcgtggtcgg cgatatcgac
     7621 agtaaagaag cgctggcgct gataaaggat aatttaagta agtttccggc taacaaagca
     7681 gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa tatcattaat
     7741 gataaagaaa accgggtgaa cggcatcgca ctctattatc gcctgccaat ggtacaagtg
     7801 aacgatgagc aaagctttgt cgaacaagct gaatggagca tgttagttca gctgttcaat
     7861 caacgtctgc aggaacgcat acagtcgggc gagttgaaga ctatttctgg cggcactgcg
     7921 cgcagcgtta aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa tgcacgagac
     7981 gataatatgc aggatgctgc gaatgcatta atggcagagt tggcaaccat tgatcagcat
     8041 ggtttttctg ctgaagaact cgatgatgtc aaatctaccc gcctcacctg gctgaaaaat
     8101 gcggttgatc agcaagctga acgtgattta cgtatgctga ccagtcgcct ggcatccagc
     8161 tcattaaata atacgccgtt cttgtcgccg gaagagacat atcaactttc gaaacgtctg
     8221 tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc agcagttaag aaagaaccag
     8281 gacgcatttt gggagcaaat ggtaaacaat gagcttgccg ccaaaaaagc attgtctcct
     8341 gcggctatcc tggcgctgga aaaagagtac gccaacaaaa agctggcggc ttacatcttc
     8401 ccaggcagaa atttatcgtt aacagtagac gctgacccac aggcggaaat tagcagcaaa
     8461 gaaacgctgg ctgagaatct gacatcatta acactttcca atggtgccaa ggttattctg
     8521 gcaaaatccg cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa taaaggcgat
     8581 ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg caaataaagc agttagcgga
     8641 agcggcgttg gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga aaactcggta
     8701 accatgagca gtaaagtcag tggcatgaat acgttgctct ccgttagcgc gcggactaat
     8761 aaccctgaac ctggtttcca gttgattaac cagcgaatca cccacagcac gattaacgac
     8821 aatatttggg catcgctaca aaatgctcaa attcaggcgt tgaaaacgct cgaccagcgt
     8881 ccagcggaga aattcgccca gctgatgtat gagacgcgct atgctgatga ccgcacgaaa
     8941 ttactgcaag aaaatcagat tgcacagttt actgccgcag atgcgctggc tgccgatcgc
     9001 cagttgtttt catctccagc ggatatcacg tttgtcattg tcggtaatgt cgcagaagac
     9061 aaactcgtgg cgttaattac gcgttactta ggctcaatca aacactctga ttcgccatta
     9121 gccgcaggta aaccattaac tcgcgcgacg gacaacgcat cggttactgt aaaagaacaa
     9181 aatgaacctg tggcacaggt ttcacagtgg aagcgttatg attcccggac acctgttaat
     9241 ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga tctacgcgtt
     9301 aatattcgtg aacaggcatc tggggcatac agcgtttctt ctcgcctctc ggttgatcct
     9361 caggctaaag atatcagtca tttgctggct tttacttgtc aaccagaacg acatgatgaa
     9421 cagttaacgt tagcgaatga agtgatggtt aagcgtttga ctaaggggat cagtgagcaa
     9481 gaactgaacg aataccagca aaacgttcag cgcagcctcg atatccaaca gcgtagcgtt
     9541 caacaattag cgaacactat tataaatagt cttattcaat atgacgatcc tgcagcatgg
     9601 actgagcagg agcaattgtt gaaacaaatg acgatagaga atgttaacac tgccgttaaa
     9661 caatatcttt ctcatccggt aaatacttat accggagtat tattgccaaa ataataacag
     9721 ccccgtcaac acatcgttgg cggggatttt agcaatattc gctattttta tgtaataatt
     9781 ttataaatgc gttcaaaata ataaccaagt actaatagtg atattttaat gtctgatttt
     9841 tacgtgataa ttcaggaggc acagaaagcg cataaaaata acaacataaa acaccttgcc
     9901 accacccaaa aattctctat tgcattgttt ttaatgaaaa aaatatcatt ccaacaatat
     9961 ctcacgataa ataacattag gattttgtta tttaaacacg agtcctttgc acttgcttac
    10021 tttatcgata aatcctactt ttttaatgcg atccaatcat tttaaggagt ttaaaatgga
    10081 taagaagcaa gtaacggatt taaggtcgga actactcgat tcacgttttg gtgcgaagtc
    10141 tatttccact atcgcagaat caaaacgttt tccgctgcac gaaatgcgcg acgatgtcgc
    10201 atttcagatt atcaatgatg aattatatct tgatggcaac gctcgtcaga acctggccac
    10261 tttctgccag acctgggacg acgaaaacgt ccacaagttg atggatttat ccattaacaa
    10321 aaactggatc gacaaagaag aatatccgca atccgcagcc atcgacctgc gttgcgtaaa
//
LOCUS       DAJTNM010000129        10256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTNM010000129 DAJTNM010000000
VERSION     DAJTNM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.129"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            80..883
                     /gene="hpaI"
                     /locus_tag="OO172_003427"
     CDS             80..883
                     /gene="hpaI"
                     /locus_tag="OO172_003427"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5513482.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            905..2260
                     /gene="hpaX"
                     /locus_tag="OO172_003428"
     CDS             905..2260
                     /gene="hpaX"
                     /locus_tag="OO172_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5513483.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2270..3160
                     /gene="hpaA"
                     /locus_tag="OO172_003429"
     CDS             2270..3160
                     /gene="hpaA"
                     /locus_tag="OO172_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5513484.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3187..4124
                     /locus_tag="OO172_003430"
                     /pseudo
     CDS             3187..4124
                     /locus_tag="OO172_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4351..5909
                     /gene="hpaB"
                     /locus_tag="OO172_003431"
                     /pseudo
     CDS             4351..5909
                     /gene="hpaB"
                     /locus_tag="OO172_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5927..6439
                     /locus_tag="OO172_003432"
     CDS             5927..6439
                     /locus_tag="OO172_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5513485.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6832..8982
                     /gene="btsT"
                     /locus_tag="OO172_003433"
     CDS             6832..8982
                     /gene="btsT"
                     /locus_tag="OO172_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5513486.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            9032..9235
                     /locus_tag="OO172_003434"
     CDS             9032..9235
                     /locus_tag="OO172_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5513487.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9246..10202
                     /gene="yjiA"
                     /locus_tag="OO172_003435"
     CDS             9246..10202
                     /gene="yjiA"
                     /locus_tag="OO172_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5513488.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2445 a         2670 c         2650 g         2491 t
ORIGIN      
        1 agccccgaaa aagagccagt cagttgctta actgactggc attactccat tagctgccgc
       61 tttgtttaag gagagaacga tggaaaacag ttttaaagcg gcgctgaaag caggccgtcc
      121 gcagattgga ttatggctgg ggctgagcag cagctacagc gcggagttac tggccggacc
      181 aggattcgac tggttattga tcgacggtga acacgcgccg aataacgtgc aaaccgtgct
      241 cacccaacta caggcgattg cgccctaccc cagccagcca gtggtacgcc catcgtggaa
      301 cgatccggtg caaatcaaac aactgctgga cgtaggcaca caaaccttac tggtgccgat
      361 ggtacaaaac gccgacgaag cccgtaaagc ggtacgcgcc acccgttatc cccccgccgg
      421 tattcgcggt gtgggcagtg cgctggctcg cgcctcgcgc tggaatcgca ttcctgatta
      481 cctgcaaaaa gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat
      541 gaagaactta ccgcagattc tggacgtgga aggggtcgac ggcgtgttta tcggcccggc
      601 ggatctgagc gccgatatgg gttatgccgg taatccgcag cacccggaag tacaggccgc
      661 cattgagcag gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc
      721 caatgagcaa ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt
      781 tgacaccacc ctgctcgccc gcgccgccga agcgctggca gcacggtttg gtaatagttc
      841 ttctatttcg atggcgaagc aaaacaacaa ctctgtctac taaacgtacg atgtggagcg
      901 catcatgacg acctctaccc tgcaatataa agataataaa actgttgaaa ttgaacatcg
      961 tgtcattaat aaattatttc gtcgcctaat catttttctt tttattctgt ttgttttttc
     1021 gtttcttgat cgcatcaata taggatttgc cggactgaca atgggcaagg atcttggcct
     1081 gacgtccaca atgtttggcc tcgccgcgac actcttttat gtcacgtacg tcatgtgcgg
     1141 gatccccagt aatatgatgt tagcgaaaat tggcgctcgt cgatggatcg ctgggatcat
     1201 ggtggtctgg ggaattgctt ccacctgcac aatgtttgca acaagccccg aaacactcta
     1261 tctactgcgt atgctggttg gtattgctga ggcgggtttt ttacctggta ttttggttta
     1321 tctgacctgg tggttcccgg catatcatcg ggccagagcc aatgccttat ttatgatagc
     1381 gatgccagtc actatgatgc tgggattgat cctttcaggc tacattctgg ccttggatgg
     1441 actgtggaat ctaaaaggct ggcaatggct ctttttgctg gaaggtctgc catcggtcat
     1501 tcttggtgtc gtcacctggt tttatctcaa cgacacacca gaccaggcga cctggcttga
     1561 tgaaaacgaa aaacaggctt taaaaacgat gattctccgt gaacaagaga gtgcatttgc
     1621 ccatgcatct gttccacgct caaccttacg tgaagtcctg accccagcca tcatgctata
     1681 tacacttgct tatttttgcc tgactaatac tctgagtgcg attaatatct ggacgccgca
     1741 aatcctacag agtttcaata ctggcagtag caacatcatg attggtttgc tggcggccat
     1801 cccccaattc tgcacaattt ttggcatgat ctggtggagt cgtcgttccg accgtcttaa
     1861 agaacgcaaa aagcatacga ttcttcccta cctgtttgcc gctgccggat ggttactcgc
     1921 ttctgcgaca gatcacagcc tcattcaact gagcggcatc attatggcct caatgggatc
     1981 atttaccgcg atgactatct tttggacaac acaggatcaa gttataagct tgcaatcgcg
     2041 tgccgttgcg atggctgtca tcaatgctgt cggcaatgta ggttcagcag tcagtccatt
     2101 acttatcggt attttgaaag atgcaaccgg cagttttagc tcaggtttgt ggtttgtagc
     2161 aggattgctg gtactgggcg cgttagtcat gacccgaatc cctatgggga gtcgggaaca
     2221 ctctctttca tctgaactta tcgcaccaaa accacaataa ggagtcatga tgtccagtga
     2281 agctatcttt gaacatggtt atgaaaatat tgatttggtc cgggaatatg atgcccgcta
     2341 cgctggtgaa gatgtgcatt atgaaacatt tacccggtta gcgaatttct ttggacgcga
     2401 tatgcgccct cactggcacg atagatattt tcaactccat tatctggtca ctggacgcat
     2461 tactttacaa ctggatgagc acttctattc tgtgcgcgca ccgctttttg tactaacacc
     2521 accatcggtg ccacatactt tctttactga tcctgatagc gatgggcatg ttctgaccgt
     2581 acgtcaggag ttaatttggc ccctggtaga aaagctctgg cctggtagtg gagatgccgc
     2641 aatgctacaa ggtatctgtc tgtcaccgga gggaatggaa catacactgg cggcattaaa
     2701 tcattattgg ccgataatat ccctggagtt tcaacaaaat aagccaggac gggaaattct
     2761 attgcaatca ctggcgcaaa gtatttttac aatcctactg cgtgaagcac caccaaatga
     2821 tatttcaaca tgcagcgtgc gcggcgagat gtgtttattc cagaagttta atcggctgat
     2881 tgacgaaaat tatcgccaac atttaatggt ccctgaatat gcaacaatgc tcggtatgag
     2941 tgaatcacgg cttaccgagc tgtgccgacg ttttgccaat caatcgccga agagattgat
     3001 tttcgaacga gtgagccggg aagccagacg gcttctgctc tatagcgagc aaagcatcaa
     3061 ccagatagcc cttgatcttg gctacaaaga tcccgcctat ttcgcccgtt ttttcaaccg
     3121 aatgatgggt tgctcaccaa cacaattccg tggtcgctga ccatttatca acctgagaga
     3181 ctcgacatga aaatatcttt acgctcacta tttgtggcgt gcctgattgc gccagtggca
     3241 ttcacctacg cctccgaacc gcaataaatg aaaattgtac tacttggaac gaaaggtggc
     3301 ccttctttat taaaagctga ccgtttaccg atggcaacgg cacttattat tggcaataaa
     3361 acctggttaa ttgatgctgg ttacggggcc agcttacaac tggtaaaaaa taatattcca
     3421 ctgcgccaga ttgataatat tttactcacc catttacaca gtgatcatat tctggactat
     3481 ccatcagtct taatgaatgc atgggctagc ggtctgcagg acaaaactat tcatgtattc
     3541 ggtccgccag gcacccgagc catgaccgct gccagttggc aggtcttcga tcgggacatc
     3601 acactgcgta tggaagaaga aggaaaaccc gatcctcgca aactcgttac agctcaagat
     3661 gttggtgaag gcgtagttta taaagatgat accgtgaccg tgactgcatt aaaggtccct
     3721 cattctcctt tccctgacgg tgaggctttt gcttatcgct tcgacacaca aggcaagcga
     3781 attgtcttct caggagatac ctcgtacttt cccccactgg caactttctc agaaggcgct
     3841 gatatacttg tgcatgaggc cgtacatgag ccctccgtcg ctaagctggc agacagtatt
     3901 ggcaatggta aaacactggc aaaagccatt gcttctcacc atacgactat tgaggatgta
     3961 ggcaaaattg ccaatgaagc aaaagtgaaa aaactcatcc ttagccacct ggtaccaatc
     4021 accgttccgg atgaaacatg gaagcaagaa gcacagaaaa ctattcagtc cctatcgtcg
     4081 ttggtcatga aaatatgacg attaccattc cattcgcccc ataaaaaatt ccgtccccgt
     4141 tagcccttgt tagcggggaa tattatgatt tgccttactg taagttgatc acgatcgcag
     4201 tttactattt aactccacct tgctgtcgaa aagtaccaac gttcgcctga accgtctctt
     4261 cccgacaacc gcgttcaacg cttcaattaa ataacaaaaa caacacacaa aattaacaat
     4321 cgtattccga ttaatactgt agaggtcgac atgaaaccag aagatttccg cgccagtacc
     4381 caacgtcctt tcaccgggga agagtatctg aaaagcctgc aggatggtcg cgagatctat
     4441 atctatggcg agcgagtgaa agacgtcacc actcatccgg catttcgtaa tgcggcagcg
     4501 tctgttgccc aactgtacga cgcgctgcac aaaccggaga tgcaggactc tctgtgttgg
     4561 aacaccgaca ccggcagcgg cggctatacc cataaattct tccgcgtggc gaaaagtgcc
     4621 gacgacctgc gccagcaacg cgacgccatc gctgaatggt cacgcctgag ctatggctgg
     4681 atgggccgta ccccagacta caaagccgct ttcggttgcg cactgggcgc gaatccgggc
     4741 ttttacggtc agttcgagca gaacgcccgt aactggtata cccgtattca ggaaactggc
     4801 ctctacttta accacgcgat tgttaaccca ccgatcgtca tttgccgacc gataaagtga
     4861 aagacgttta catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga
     4921 aagtggttgc caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac
     4981 aagtgatggg cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg
     5041 gcgtgaaatt aatctcccgc gcctcttatg agatggtcgc gggtgctacc ggctcgccat
     5101 acgactaccc gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg
     5161 tactgatccc atgggaaaac gtgctgatct accgcgattt tgatcgctgc cgtcgctgga
     5221 cgatggaagg cggtttcgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga
     5281 aactcgactt cattacggca ctgctgaaaa aatcactcga atgtaccggc accctggagt
     5341 tccgtggtgt gcaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat
     5401 tgagtgactc gatgtgttca gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc
     5461 atgccgcact gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca
     5521 ttatagaacg caacgttacc agtggcctga tctacctccc ttccagtgcc cgtgacctga
     5581 acaatccgca gatcgaccag tatctggcga agtatgtgcg cggttcgaac ggtatggatc
     5641 acgtccagcg catcaagatc ctcaaactga tgtgggacgc cattggcagc gaatttggtg
     5701 gtcgtcacga gctgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcaat
     5761 gtctgcgcca ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct
     5821 gcctgtcgga atacgaccag aacggctgga ctgtgccgca cctgcacaac aacgacgata
     5881 tcaacatgct ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga
     5941 acaacgcctg cgctttcatg acgcgatggt cagcctgtcg gcagcggtaa atattatcac
     6001 caccgagggc gacgccggac aatgcgggat tacggcaacg gctgtctgct cggtcacgga
     6061 tacaccaccg tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
     6121 gggcaacggc aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcgcgcca
     6181 cttcgcgggc atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
     6241 aggtccgctg gcgcagccgg tgctaaaagg ttcgctggcc agtcttgaag gagagatccg
     6301 cgatgtgcag gcgattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
     6361 cagtgcagaa ggtcacggac ttatttactt taaacgccgt ttccatccgg tgatgctgga
     6421 aatggaagct gcgatttaag ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat
     6481 tgtgatctca atcacatatc tacccgatga ataattcttg cccaaccgct caccgccatt
     6541 cctgaccgct tacatcccta aaataaccac tcagttattt accttacttt acgcgcacgt
     6601 atctctggca acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac
     6661 ctagaacggc ttcggcctat gattaacaaa ttactctatg gattttggtt tgcaggaagg
     6721 cagcaagtga gtgaatcccc gggagcttac aaaagtaagt gtcagaggtg aacgaacgca
     6781 gctgccgcac ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg
     6841 aaaaaactat tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc
     6901 gcggtagttg cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct
     6961 gtgtcggtgt atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg
     7021 aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt
     7081 ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg
     7141 ctggtgggac cggttctcgc cgcgcagatg ggctacctgc ctggcacgct gtggctgctg
     7201 gcgggggtag tgctggccgg tgcggtacag gactttatgg tgctgtttat ctcctctcgc
     7261 cgtaacggcg catctctcgg tgagatgatc aaagaagaga tggggccagt accggggact
     7321 atcgcgctgt ttggctgttt cttaatcatg atcatcatcc tcgccgttct ggcgctgatt
     7381 gtggttaaag ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg
     7441 attgcgctgt ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc
     7501 tctgttattg gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac
     7561 gatccgtact ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt
     7621 ggctatgcgt ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat
     7681 ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg
     7741 aacccggaac tgaaaatgcc tgcgatgacc cagtacattg acggtactgg cccgctgtgg
     7801 aaaggcgctc tattcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc
     7861 cacgcgctga tctcttccgg tacgacgccg aaactgctgg ctaacgaaac cgacgcgcgt
     7921 ttcattggct acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgca
     7981 gcgtccatca tcgaaccggg tctttacttc gcgatgaaca ccccgcctgc tggccttggc
     8041 atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag
     8101 ctgaaagacg ttaccgcaca cgcggcagcg accgtcagct cctggggctt cgtgatttcg
     8161 ccagagcaga tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca
     8221 ggtggtgcgc cgacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg
     8281 gctgacatgg gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc
     8341 gcgctggatg cgggtacccg ttctggccgc tttatgctgc aagacctgct gggtaacttc
     8401 atcccgttcc tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt
     8461 gtgggtctgt ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag
     8521 agcctgtggc cgctgttcgg tatctccaac cagatgctgg cagccgtagc gctggtactg
     8581 ggcaccgttg tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg
     8641 gctgtatggc tgcttatctg caccacctgg gcgctgggtc tgaaactgtt cagcaccaac
     8701 ccgcagatgg aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt
     8761 actgacctga cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc
     8821 aacgcaggcc tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc
     8881 aaaacctggc ttgcggtgcg taacagcgac aaacgtactg acaaagaaac accgtacgtt
     8941 ccaatcccgg aaggcggcgt gaagatctct tcgcaccact aaccgtgttt agccccgctt
     9001 cggcggggct ttgttcttat cagagtgaac tatgtttggt aacttaggac aggcaaaaaa
     9061 atatctcggc caggcggcga agatgttgat tggtattcca gactacgaca actacgtcga
     9121 gcatatgaag accaaccatc ccgacaagcc gtacatgagc tatgaagaat tcttccgcga
     9181 acgccagaac gcgcgctacg gcggcgatgg taaaggcggt atgcgctgtt gttaatggag
     9241 agagcatgaa cccgattgca gttaccctac tcaccggttt tttaggcgcg ggcaaaacta
     9301 ccctgctgcg ccatattctt aacgaacaac acggctacaa gattgccgtg attgaaaacg
     9361 aattcggcga agtctccgtt gatgatcaac tgattggcga ccgcgccacg cagatcaaaa
     9421 cgctgaccaa cggctgcatc tgctgttcgc gctccaacga gctggaggac gcactactcg
     9481 acctgctgga taatctcgac aagggcaata ttcagttcga tcgtctggtg attgaatgca
     9541 ccggcatggc cgatcccggc ccgattattc agaccttttt ctcccatgag attttatgcc
     9601 agcgctacct gctggacggc gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga
     9661 tgaaccagtt caccatcgcc cagtcccagg tgggctacgc cgaccgtatt ctgctgacta
     9721 aaaccgacgt cgcaggcgaa gcagagaaac tgcgtgaacg cctggcgcgc atcaacgccc
     9781 gcgcaccggt ctacaccgtc acccacggcg acatcgacct gggcctgctg ttcaacacca
     9841 acggttttat gctggaagag aacgtcgtca gcaccaaacc gcgtttccac tttatcgcgg
     9901 ataaacaaaa cgatatttcg tcaattgtgg tggaactgga ttacccggta gatatcagcg
     9961 aagtttcccg cgtgatggaa aacctgctgc tggaatcggc ggataaactg ctgcgttaca
    10021 aagggatgct gtggattgac ggcgaaccta accgcctgct gttccagggc gtccagcgcc
    10081 tctacagcgc cgactgggac aggccgtggg gcgatgaaaa accgcatagc acaatggtgt
    10141 ttatcgggat tcagttgccg gaggaagaga ttcgggcggc gtttgcggga ttgaggaagt
    10201 aagttcggga aagatcaact ttttgagagt tccaccgcaa cattattacg agtgga
//
LOCUS       DAJTNM010000130        10191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTNM010000130 DAJTNM010000000
VERSION     DAJTNM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.130"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            301..1017
                     /locus_tag="OO172_003436"
     CDS             301..1017
                     /locus_tag="OO172_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5513489.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLDAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1473..1548)
                     /locus_tag="OO172_003437"
     tRNA            complement(1473..1548)
                     /locus_tag="OO172_003437"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1513..1515),aa:Asn,seq:gtt)
     gene            complement(1649..3276)
                     /gene="yeeO"
                     /locus_tag="OO172_003438"
                     /pseudo
     CDS             complement(1649..3276)
                     /gene="yeeO"
                     /locus_tag="OO172_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3281..3356
                     /locus_tag="OO172_003439"
     tRNA            3281..3356
                     /locus_tag="OO172_003439"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3314..3316,aa:Asn,seq:gtt)
     gene            complement(3394..4344)
                     /gene="cbl"
                     /locus_tag="OO172_003440"
     CDS             complement(3394..4344)
                     /gene="cbl"
                     /locus_tag="OO172_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5513490.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4446..5363)
                     /gene="nac"
                     /locus_tag="OO172_003441"
     CDS             complement(4446..5363)
                     /gene="nac"
                     /locus_tag="OO172_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5513491.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            5690..5765
                     /locus_tag="OO172_003442"
     tRNA            5690..5765
                     /locus_tag="OO172_003442"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5723..5725,aa:Asn,seq:gtt)
     gene            complement(5821..6756)
                     /gene="ldtA"
                     /locus_tag="OO172_003443"
     CDS             complement(5821..6756)
                     /gene="ldtA"
                     /locus_tag="OO172_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5513492.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(6818..7897)
                     /gene="cobT"
                     /locus_tag="OO172_003444"
     CDS             complement(6818..7897)
                     /gene="cobT"
                     /locus_tag="OO172_003444"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5513493.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(7909..8641)
                     /gene="cobS"
                     /locus_tag="OO172_003445"
                     /pseudo
     CDS             complement(7909..8641)
                     /gene="cobS"
                     /locus_tag="OO172_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
     gene            complement(8638..9183)
                     /gene="cobU"
                     /locus_tag="OO172_003446"
     CDS             complement(8638..9183)
                     /gene="cobU"
                     /locus_tag="OO172_003446"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5513494.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(9810..10073)
                     /locus_tag="OO172_003447"
                     /pseudo
     CDS             complement(9810..10073)
                     /locus_tag="OO172_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2899 a         2583 c         2160 g         2549 t
ORIGIN      
        1 agtaagacat ctggcaaata gcctgcaata aacgcgggct gcttgcttcc ttgggcagat
       61 gcgagtttta ttatcgtctt aatgatttcc acatattaaa agcaagtatg ctttcaaaac
      121 acaattataa aaaatcccgc caacaacatt agtttttata aaattaaata taagattatg
      181 gctttagaat atttttattt ctaatagacg agatttttcc tgttatgata taatatgccg
      241 aattaacaca tgttaacgat ttaccagtaa tgtaaataaa ttttcgagga gatcattcca
      301 gtgggacgta aatgggccaa tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct
      361 aaaatttatg caaaattcgg tgtagaaatc tatgctgctg ccaaacaagg tgaacccgat
      421 ccagaattaa acacatcttt aaaattcgtt attgaacgtg caaagcaggc acaagttcca
      481 aagcacgtta ttgataaagc aattgataaa gccaaaggcg gcggagatga aacgttcgta
      541 cagggacgtt atgaaggctt tggtcctaat ggctcaatga ttattgctga gacattgact
      601 tcaaatgtta accgtacgat tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat
      661 atcggagcgg caggttctgt cagctatatg tttgacaata cgggtgtgat tgtatttaaa
      721 ggaacagacc ctgaccatat ttttgaaatt ttgcttgacg ctgaagttga tgttcgtgat
      781 gtaactgaag aagaaggtaa tattgttatt tatactgaac ctgctgacct tcataaagga
      841 atcgcggcgc taaaagcagc tggaatcact gagttctcaa caacagaatt agaaatgatt
      901 gctcaatctg aagttgagct ttccccagaa gatttagaaa tctttgaagg gcttgttgat
      961 gcccttgaag atgacgacga tgtacaaaaa gtttatcata acgtcgcaaa tctctaatta
     1021 tattttaaag aaatctgtct ttatggcaga tttctttaat ctcatataat tcttataaaa
     1081 aatataatgt ttaactcgtc atattgctta taaaaaatcc ccgttcccag agaaataata
     1141 tttattaaaa tggcaggtct tctttttctg attaccgaag acaaagtggc aattacgtat
     1201 agtttcccga taaagacgcg acagctacat cccgcataag gcatttttct ctttatcttt
     1261 gtacggtact tcatggaaca gaatttttga ccttgtgaat cgtgatgtct gttggggagg
     1321 ggcaatttgc tcactgaagc gtgagactcg attaagcgca cgaaacacag aaatcaaaaa
     1381 acccggccac ttttttacaa agtggccggg taaaaataat ttttattttt taactgtcct
     1441 gagtctcata gagatgtctc aaaactaaaa tttggctcct ctgactggac tcgaaccagt
     1501 gacatacgga ttaacagtcc gccgttctac cgactgaact acagaggaat cgtgagaacg
     1561 aggcgaatat tagcgatgcc cacccacaat gtcaaagcct gttttttaaa tttgaaatcg
     1621 tttgctgaaa taatctgcat tttgtcgttt attccgacac aactggcttt ttttcacact
     1681 tttgcggctc ggatcgaggg tatttccaca gccaacgtcc agtaaccatt cgccagtaaa
     1741 acagcacggc ccgcacagcc cagtaggcaa acattcccat ccagacacca accacacccc
     1801 agccaagcat gattccctgc acataaccga ctacaacccg gcaaccccac atgctcaaca
     1861 tagaaaccca catggcgtaa cgggcatcac gagcaccctt aaatccagcg ggtagcaccc
     1921 atgaggcgga ccaaataggc ataaataaag catttagcta aatcagaatc acaacgacgt
     1981 gtttaacctg tggatcctgg gtgtaaaacg atgccataac cccggcaaag ggagccgtta
     2041 gccaggcgat ggccgttaat ccaagagtgg acagccagaa cacatgccgc aactgaatct
     2101 ctgcttgcgc tatctgccct acccccaacc ttcggcctgt aatgatcgta gaagcagaac
     2161 cgagcgcact tccgggtaag ttgataagag ccgcaattga gaacgcgata aaatttccgg
     2221 caataacgct ggtccccatc ccggcaacga acatttgggt taataaccga ccactggtaa
     2281 ataacactga ttcgacactc gctggaatac caatccccat gacttcccag ataatgctaa
     2341 aattcagcgg tttaaaatag ctctttaacg aaatccttag cgcaggatta aaaccaatcg
     2401 ccagcaccca caaaattgca actgcgccaa tataacgaga aatggttaaa cccaacccag
     2461 ccccgaccag gagaaaaggc cgtaaatcaa tatgccgcta ataataatat taagaatatt
     2521 caggctaccg ttaatcagta gcggtatttt cgtattccct gcaccacgaa gtgccccgct
     2581 accaataaga gtgatggcag cggctggata actgagtacg gtcagctcca gataagtcaa
     2641 cgccagtgct ttaacttctg tcgtggcatc acccgcgacg aaatcaataa tttgttcgcc
     2701 aaaatgatga attagcgttg ccaacagtac ggcaaacaac gtcatgatca ccaatgactg
     2761 ccgcgtcgcc accctcgctc gtcgtcgatc ccgcttaccg agactaaatg ctacaacgac
     2821 agtagtacca agatcgatag ctgcaaaaaa agccataatg accatattga agctgtccgc
     2881 caaccccacg ccggccatcg catcttttcc cagccagctg accagaaaag tgctcaggcc
     2941 ccccatcaac aggacacaga cgttctccat gaagatagga acagcaagcg gggttatctc
     3001 gcgccagaac aacactttgt agctcttgcg tttagagtgc cagcgagtgc cgtgaacaac
     3061 ctggcgtaaa gcagaggaga tattcaaagc cgaccttaat tgcagaaagt gaaaccacat
     3121 ttcaaatgat gagggagaat cagcaaagct gcaaagattt tcgccaacaa attgtctgca
     3181 aatgcaacaa actgttgata gaaacggcaa acagttggga aatttaaaaa tcgggtttga
     3241 caaaatattt ttcgccgtta agatgtgcct caacagcgat tcctctgtag ttcagtcggt
     3301 agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt
     3361 tctgaaaagc ccgcttttat agcgggcttt ttgctatatc tgataatcaa tttcctcttc
     3421 actgttttcc atcacctggc gcttgatatc ctcaactgac agtcctgcat tacaaagttc
     3481 cagaaagcgc caaacatagt tacgctgaag ttgtcctcgc ttcagtccca accagacagt
     3541 attagcatcg aaaagatgcc gcgtatccag gcggattaaa ttctcttcct cttgttcgcc
     3601 gctggattgt tcggcaacta atccgatccc aagcccaaga gcaacatagg ttttaatgac
     3661 atcagaatcc tgtgcactta atacaatatc tgccggcaaa cctttgcggg taaatgcgtc
     3721 atcagtgcgt gagcgccccg taattccctg tcggtaagta attaacggcc acttcgctat
     3781 tgattccagc gtcaatggtg taatttgcgt taagggatga tcgagtggaa caagcaaact
     3841 atggtgccaa caaaaccacg ggaaggcgac gagctgcggg tcattactca aacgctcgct
     3901 ggcgatacca atatcagctt cgccattttg caacaacgtc gcaatttcct gtggcgaacc
     3961 ctggattagc tcgagccgaa cctccgggaa aagttcgcga aaagctttaa tgacctctgg
     4021 caagctataa cgtgcctgag tatgcgtcgt tgcaatagtg agaacaccag acgtatcgtt
     4081 ggtaaacagg tctgcaagcc gacgaacatt actggcttca ttcagaatac gttctgcaat
     4141 gaccagtaat gctttgcccg gttcagtcat ccccagcagt cgcttacctc gtcgaacaaa
     4201 tatttcgatg ccaagttcat cctccagttc ccgaatatga cggctaacgc ctgactgtga
     4261 ggtaaaaagc atattcgcaa cctctgtcag gttgtaatcc tgacgtgcag cctcgcggat
     4321 tatctttagt tgttggaaat tcacggtaaa ctccgggcag ttcagatatc ccgttattgt
     4381 taaagtctaa tgcccggcat aacaaataat aaaaacccgc atcttattcc atcccgatat
     4441 aacgcttagc tcaccaattg ccactgcctt ttttccatca ctggagaact aatcactgac
     4501 attaacaact ctttcactgc ctgtgcctgt ggtgacaagt ttgctctggc gggtaaattt
     4561 aaggacaaag agagactcat tgaaggagtg gtaatgcgtg acatccaccc atttactgcg
     4621 ccacataacg aacgcgcagc cgattcaggt aatactgcaa cgcccatgcc gctggcaatc
     4681 gctgcggtaa gcgtggcaat agactcaatt tcaccaatga cttttgccgt gagccgccgt
     4741 agggaaaaag cctcatcaac acgaagtcta acagcgctgt aatcactggg gagaaaaagg
     4801 ttcatttgcg caatagcatt aacatcaacg ttttgccccg ggcaatcttg agttcctacc
     4861 aggaaaagat cttctttcag caaagcctga ctggataccc cagccacagg ggaatgctca
     4921 taaatcaccg ccatatcgag ttggtgattt atcagttttt cgttaagcac tgcgccacta
     4981 ttttcatgaa gatagataac gacctccggg aattcagcgc gaaccgcctg taataagggc
     5041 atggtgatgg atgacgcagc ggttcctggc gcaaagccaa tcgagacttg ccccgataat
     5101 gactgaccaa cgttatgcac cgccagttgg gcctgctcac actgacgtaa aatggcccgt
     5161 gcatgggtat agagaatttt tccggcgtct gttggcgtaa cgccccgttt tgtgcgaatc
     5221 aaaagctgtt gatttaactc accttccagt atggcaacct gctggctgag cgctggttgt
     5281 gcgatatgca atacttcagc agcctgggtc aggctaccaa tatctacaat ttttacgaag
     5341 tatttcaggc gtctgaagtt catgttgcct ccggttttta agaatcggcc caagtgccgc
     5401 cattacttac aaccagtatg caagatgcct gccagttcta ttttggcgtt gatgcgcaag
     5461 ctaaccaact gtcaaataag agattatgat agattcgtca ttggcccttt ttatcagcag
     5521 tcgcattcac ctgccctata aaaggagggt atgcaccacg atggttcatt acccaataag
     5581 attgaaagct caccactttg ttgaaattgg cagcaaacaa acaaaaaaat gcatttcacc
     5641 ctttgacatc accatgcact gccattaata tgcgccccgt tcacacgatt cctctgtagt
     5701 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
     5761 agccaaattc aaaaaagcct gctttcgagc aggcttttta ctttctaatt accaacgctc
     5821 ttaaaacatc tgtcttgaac cagaactaat ttgcacaggc attcccgatc gatgttgcaa
     5881 cgcagcattt gcgcgattta catcaacttc ttgcccgttg ataaacgccc gcaaagatgg
     5941 ggttaccggc aatggcactt ttcggtcaga ctcatattct gcacgattgc gtgacagcgg
     6001 ctcatgaact tccagccact ttgaaccatc cggttccgtt gtgtatttca ctggctgatc
     6061 aataatttgt acacgtgtgc ctacaggaac attatcaaac agatatttga tatcgtcatt
     6121 acgcagacga atacagccct gacttacacg gagcccaata ccaaaattgg cattggtacc
     6181 gtggatggca tacaacctgc caatataaat cgcgtacagc cccatggggt tatcgggccc
     6241 cgcaggaaca aatgcgggca aactctcccc tcgtttcgca tattcgcgcc gggtgttcgg
     6301 cgttggcgtc caggttggtg cttcttgttt acgttcaacg gtagtcaccc agttacgcgg
     6361 ggtttctcgc ccagcctggc cgataccaat aggaaagact tccactgtat tactgtctgg
     6421 cggatagtaa taaagacgca tttcagcgac gttaaccaca atccctttac gaacagtggc
     6481 gggcaaaatc agttgctgcg gaatggtgag ttgtgagcca ggcttcggta aaaaaacatc
     6541 agcgcccgga ttcgcttcca gcatattact taacccttgc ccgtattgtg cggcaaaagt
     6601 ctccagcggc tgagtattgt gatcaggaac agttacagta agcgactgcc ccactaaacg
     6661 gctaccctct ggaggtaatg gataagttac cgccaggcta ttttggctgg caaaaagcag
     6721 agcaaatgag caaagaatat ttacacgacg catcatgtcc ctttcctaag tcgcgaatac
     6781 tatccgataa gtatagcttt tatcagactt ttcgttttta gctgttcaaa tcagaagtcg
     6841 tattccccgg tagaacaata ttactggcag caagttcgcc catgttgttg tatatcgcac
     6901 aagcagcttc gatgatggac atcgccagag ctgcaccact cccctcacct aaacgcatat
     6961 ccatattgag ataaggctcc agccccaaat gcgagagcgc tatacgcgcg cctttttctg
     7021 ccgacaagtg agaaggaatg agatacggtt tgattgcagg agacatctgg caggctgcga
     7081 gcgcagcagc ataagaaaga aatccatcca gcaacacggg taaaccgcag gaagcagcac
     7141 ctaacatcac tccagctatt ccgaccaaat caaatccacc cacttttgcc aggacatcaa
     7201 caccatcctg aggatttggt tgattcaacg taatcgcccg acgcacaaca tcaattttat
     7261 tagccagttt atctgtcggc aggtttgcgc caatcccaac cacttcttca ggatcccggc
     7321 cagtgattgt gctgactatt gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta
     7381 caccaaacag cgtgacaccg tttttcgcca gctcccgcgt ataacatatg acgtccaaaa
     7441 gcaacttttc agcctgacgg cgactcattg ccggagctga agcaatattg ccgctacctc
     7501 gtgcgacacg catgttgata agcccgggga taggctcagc agtatcaata ccaacatcaa
     7561 ttacgtggac gttagcgccc gcttgtgctg ccagcacaca cacgccggtt gttccacggg
     7621 tcatattttc agcctgtatg gctgtcactt cttttggaga aatagcgacc ccttcctccc
     7681 agacgccgtg atcggcacac ataaccagta ccgctttttt gcccacatgc ggtatgccat
     7741 tcacccccgg cattcctgcc agttgtatgg caagcgcctc cagctttcct aggctaccaa
     7801 caggtttgag taacccgtca acatgccgtt gtgcacgcga catagcggca gaattgatgg
     7861 ctgggatcgt attcagtaaa tcggcaatta tttgcatgtc atgtctcttt atagcagtgc
     7921 cagcaggaag accagttcac caagctctat ggctgctccc agcgtatcgc ccgtttgccc
     7981 gcccagcgta cgtttgagaa gctgaccgag gatgaaaatc gccaccatcg tcaccaccat
     8041 agcagccaca ccatgcatac ccagcaataa tacggcagca aaaatagcgg ctaagccgag
     8101 tgtgacgcag gtttgtcgcg ccaatgaaca cattgccaag cccttcttcc cgcgcataac
     8161 gatgacgata catcaataat gcggcagtcc cgcggctgac cgcacatgct gccgccagcg
     8221 atgcaaggat cggttcgcca cgcagagcca actcgcttaa caccagaatc tttgccagta
     8281 ccacaaaaat caatgccaga ccgccgtggg tgcctaaacg gctatcacgc atgatttcca
     8341 acatgcgatc gcggctacgt gcagaaaata cgccatcaca ggtatcagcg aggccgtcca
     8401 gatggaattc cccggtcatc agcgccagca caagtacgct aaacagtgcc gccagtggtg
     8461 cgccacacca tgcctgcagc accatgaaga ccagcccgct aatcgcgcca agcaataatc
     8521 caatcaaagg aaaagtaata ataccgcgag aataatgctc gaaatccagt ccctgggacc
     8581 agcgacgcgg tacaggcaga cgcgtaataa atgagagcat cgcccaaaat aatttgctca
     8641 tttgattttt actccaatac ccgaaaccac cagccatact tcatttgccg cagcggccaa
     8701 ctgctgattt acccgcccgg caatatcacg aaaatgtcgt gccagacgac tctccggcac
     8761 aatccccatt cccacttcgt tagtcactaa tacaaccttt gcggggcaac gttggcaggc
     8821 agcaatcaac gactgaatct cagcattaat cgcctgttcc atcgcctgat aatcccattc
     8881 atcagggtct ttatcgccgc cataatcaaa caacagatta gtcaccattg tggtaacgca
     8941 ttccagcaac acagcctcat tcgggttaat gtctgcatga attaattcat caagatgttg
     9001 ccagcgctcc actgtgcgcc agtgctccgg gcggctttgc cgatgatgtt ctatccgtgc
     9061 agccatctca tcatcaagga tttgcgaggt agcgatatac agaacctgtg aagagtcccc
     9121 aataagcacc tctgcatggc gactcttccc gctccatgcg cctcccgtca ccaaaatcat
     9181 cataccgact cctgattcaa atgtatgatg gtatacattg aacagagcaa tggatccccc
     9241 accgttgctg accgcaaaac atttacgcga ttaatactgc gcgtaatata attaaaaatc
     9301 cagcattctc aatccattac gaaataagat ggcattacgg aatagccaga ctttctcgtg
     9361 ttaacgtccg acaaaatgcg tcattgacag cggtttctgt ggattgacac atttcattca
     9421 gataatgaat taatgctact gccggaccaa ccagcaaacg ggcattgtgt tctgaaatcc
     9481 aggctattga ttcaacgtca gcgacagatc tgcgctgtaa aagaacttgt aaaacaacgc
     9541 aatagaatta tccggaatgg tggcgactat gcactagaac accacaattt cgctctctcg
     9601 gtaaggcact gtcacagcat tcaacagaat gtgacttgcc agatttatta gcgccaccag
     9661 atgtttaacc gggtaaccac gacccagtcg gggatatatt ccatcttctc gctgacatca
     9721 tcaagaataa aaaggtttat cacactcaga gcaacgttct tcagcaggcg ggataattct
     9781 tcaacattta aaagatgtgt tattaaaggc atctcaaatt ggacagggct gccggttttc
     9841 tgttgcaaca ccgttgctct gcttgagaca gaactgccat atgcagcatc tgatactttg
     9901 agtttgacca aatcacagcc ccgtagctta ctgtccaaag ccatgttgaa cagagctaga
     9961 tcgcgtgttt taccttccaa ctcaagtcta atccggatcc cccagatatg agatatctga
    10021 agtggccttt tttgaccgat ggtacggtct ttattccacg gtgacgtttt catattcaaa
    10081 tctcctgcaa tgtgggagat ttgagtatgg ttgtctctca agagcgaaaa taagtcaact
    10141 atcactagtt gttgcgcatg tacgtattct gtcaattaat tattgacaat c
//
LOCUS       DAJTNM010000131         9974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTNM010000131 DAJTNM010000000
VERSION     DAJTNM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.131"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..2005
                     /gene="speF"
                     /locus_tag="OO172_003448"
     CDS             <1..2005
                     /gene="speF"
                     /locus_tag="OO172_003448"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR5513495.1"
                     /translation="NEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASHYETQLRP
                     PFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLCNADVAMG
                     DLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHK
                     SNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRAKEARPF
                     RLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLN
                     ENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFY
                     PLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQS
                     YETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPYKLLLTTPGIDARNGEYEAFGV
                     PATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLA
                     EVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHVSMNPQEA
                     NYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGCAVLRYFSALEEGIN
                     LLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2002..3321
                     /gene="potE"
                     /locus_tag="OO172_003449"
     CDS             2002..3321
                     /gene="potE"
                     /locus_tag="OO172_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5513496.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSITMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="OO172_003450"
     CDS             complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="OO172_003450"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5513497.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="OO172_003451"
     CDS             complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="OO172_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5513498.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5773..6537
                     /gene="ybfF"
                     /locus_tag="OO172_003452"
     CDS             5773..6537
                     /gene="ybfF"
                     /locus_tag="OO172_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5513499.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6677..6970
                     /gene="ybfE"
                     /locus_tag="OO172_003453"
     CDS             6677..6970
                     /gene="ybfE"
                     /locus_tag="OO172_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5513500.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7110..7640
                     /gene="fldA"
                     /locus_tag="OO172_003454"
     CDS             7110..7640
                     /gene="fldA"
                     /locus_tag="OO172_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5513501.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7850..7936
                     /gene="uof"
                     /locus_tag="OO172_003455"
     CDS             7850..7936
                     /gene="uof"
                     /locus_tag="OO172_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5513502.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7929..8375
                     /gene="fur"
                     /locus_tag="OO172_003456"
     CDS             7929..8375
                     /gene="fur"
                     /locus_tag="OO172_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5513503.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8459..8785)
                     /locus_tag="OO172_003457"
     CDS             complement(8459..8785)
                     /locus_tag="OO172_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513504.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8822..>9974)
                     /gene="chiP"
                     /locus_tag="OO172_003458"
     CDS             complement(8822..>9974)
                     /gene="chiP"
                     /locus_tag="OO172_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5513505.1"
                     /translation="MFGLDIAAFTAIEMAENGDSSHPNEIAFSKSNKAYDEDWSGDKS
                     GISLYKAAAKFKYGPVWARAGYIQPTGQTLLAPHWSFMPGTYQGAEAGANFDYGDAGA
                     LSFSYMWTNEYKAPWHLEMDEFYQNDKTTKVDYLHSIGAKYDFKNNFVLEAAFGQAEG
                     YIDQYFAKASYKFDIAGSPLTTSYQFYGTRDKVDDRSVNDLYDGTAWLQALTFGYRAA
                     DVVDLRLEGTWVKADSQQGYFLQRMTPTYASSNGRLDIWWDNRSYFNANGEKAVFFGA
                     MYDLKNWNLPGFAIGASYVYAWDAKPATWQSNPDAYNDKNRTIEESAYSLDAVYTIQD
                     GRAKGTMFKLHFTEYDNHSDIPSWGGGYGNIFQDERDVKFMVIAPFTIF"
BASE COUNT         2370 a         2517 c         2533 g         2554 t
ORIGIN      
        1 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
       61 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      121 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      181 ttttgactgc ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca
      241 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
      301 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
      361 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
      421 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
      481 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
      541 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
      601 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
      661 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
      721 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
      781 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
      841 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
      901 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
      961 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1021 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1081 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1141 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1201 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1261 agagaatcaa tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc
     1321 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     1381 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     1441 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
     1501 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga
     1561 gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg
     1621 ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag
     1681 catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct
     1741 gccggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct
     1801 gtgtgttgtt ccgggtgaaa tctggggttg tgctgttttg cgttacttca gcgctctgga
     1861 agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga
     1921 acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac
     1981 cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag
     2041 ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag
     2101 cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg
     2161 gcactggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc
     2221 ggttatgccg agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc
     2281 gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa
     2341 ctgctcggcg cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg
     2401 atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc
     2461 gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc
     2521 ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt
     2581 tcttccatca ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact
     2641 gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt
     2701 gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag
     2761 ctggcaaatt caacggcacc atttggcctg gccttcgcac agatgttcac accagaagtg
     2821 ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag
     2881 ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc
     2941 tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag
     3001 agcgggttgc cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt
     3061 aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt
     3121 attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt
     3181 gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac
     3241 ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa
     3301 ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt
     3361 tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt
     3421 tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt
     3481 cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc
     3541 caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca
     3601 gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag
     3661 tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt
     3721 agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa
     3781 tgatgccgtc cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg
     3841 cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga
     3901 aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg
     3961 cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga
     4021 acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga
     4081 cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca
     4141 gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt
     4201 tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga
     4261 ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa
     4321 cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt
     4381 ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga
     4441 gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca
     4501 ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac
     4561 catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt
     4621 ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt
     4681 cctgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag
     4741 gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg
     4801 cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc
     4861 gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg
     4921 cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct
     4981 gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg
     5041 ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg
     5101 atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc
     5161 gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct
     5221 gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg
     5281 gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc
     5341 tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt
     5401 ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga
     5461 gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc
     5521 tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc
     5581 gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa
     5641 taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta
     5701 tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa
     5761 aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat
     5821 tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct
     5881 cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca
     5941 ccaagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat
     6001 gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg
     6061 gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg
     6121 gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa
     6181 tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa
     6241 ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg
     6301 gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac
     6361 cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat
     6421 ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc
     6481 catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc
     6541 aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgagtcat
     6601 cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc
     6661 cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc
     6721 gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg
     6781 tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact
     6841 gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag
     6901 ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg
     6961 catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc
     7021 cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt
     7081 tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg
     7141 acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg
     7201 ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc
     7261 tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc
     7321 cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc
     7381 aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac
     7441 cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa
     7501 aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg
     7561 aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg
     7621 acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag
     7681 ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat
     7741 gtcccagtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac
     7801 aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat
     7861 tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca
     7921 gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc
     7981 ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag
     8041 atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg
     8101 tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat
     8161 ccgtatttga gctgacacag caacatcacc acgatcacct gatttgcctc gactgcggca
     8221 aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac
     8281 atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt
     8341 gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac
     8401 ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt
     8461 atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat
     8521 catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct
     8581 ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctggcagg
     8641 cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca
     8701 gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca
     8761 tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc
     8821 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     8881 tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     8941 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc
     9001 tgtaggcaga ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc
     9061 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
     9121 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt
     9181 tgaagtagga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
     9241 gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga
     9301 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg
     9361 tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact
     9421 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
     9481 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
     9541 agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
     9601 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
     9661 aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
     9721 tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac
     9781 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
     9841 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
     9901 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
     9961 caaggccgaa cata
//
LOCUS       DAJTNM010000132         9962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTNM010000132 DAJTNM010000000
VERSION     DAJTNM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.132"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..272)
                     /locus_tag="OO172_003459"
     CDS             complement(<1..272)
                     /locus_tag="OO172_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5513506.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="OO172_003460"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="OO172_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="OO172_003461"
     CDS             complement(1132..1338)
                     /locus_tag="OO172_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5513507.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="OO172_003462"
     CDS             complement(1445..2008)
                     /locus_tag="OO172_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5513508.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3388..5223)
                     /locus_tag="OO172_003463"
     CDS             complement(3388..5223)
                     /locus_tag="OO172_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513509.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5324..5611
                     /locus_tag="OO172_003464"
     CDS             5324..5611
                     /locus_tag="OO172_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5513510.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5583..7112
                     /locus_tag="OO172_003465"
     CDS             5583..7112
                     /locus_tag="OO172_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5513511.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7282..8499)
                     /locus_tag="OO172_003466"
     CDS             complement(7282..8499)
                     /locus_tag="OO172_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5513512.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8866..9852)
                     /locus_tag="OO172_003467"
     CDS             complement(8866..9852)
                     /locus_tag="OO172_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513513.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9849..>9962)
                     /locus_tag="OO172_003468"
     CDS             complement(9849..>9962)
                     /locus_tag="OO172_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5513514.1"
                     /translation="EISNKPYLGNMLNDFAGVDQQRVMHCRKAFSDAKVFK"
BASE COUNT         3142 a         2263 c         1846 g         2711 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaaacaaaca aataaatcat actattatag aattttctct tgccagacat
     3301 agataatacc cctgctaatt ttgctaatgg ttagcaaaat tagcagggta tataaagatt
     3361 attgacccaa aacacactca gatgaattca cgacagctca ggagggttat tcctgatgtc
     3421 atcttcacaa ataatggtta acgcatcata tatcattctg cgtctgttcc tgttttcggc
     3481 cttatcgcca tacaacatca tcacattttt aatttcatcg accggtgatt tcacatagtg
     3541 aactaataca ccaagcttct caacacattg cagtaaagta tatttcttac tgcctgtgaa
     3601 aagccctatc atagtttctt tacgttcgcg actggataat actttgtttt ccataatgac
     3661 ttctgccagc gtcttaccat cataaacata ctctttatct atcagaatat ttatcttatc
     3721 cagcaccttc tctacgtcat caagcaaacc tgtaatatat gggggcgcaa tatatggttt
     3781 cgtactgatc ttttcaagca tacaattata catcttaata acacgcatca gggtaaaatt
     3841 gttcgcatca tactgtattt caccctcctt atatacagga tcaataagtg gtattttttc
     3901 aataaatgca tcctgatagg cttttacatg gtcaagccaa taaaacaacg agccatactt
     3961 tatatttttt ctatttactt taacctccag tgctgtccga agcaacgata atgcccccct
     4021 gacatatcct gaaggtaatt caccaaacaa tccttttctt tccatgtcat cataatgctg
     4081 cagcacatca ttaattttat ttttccttat cagacaaatg tacaccgcaa gtgtttcgta
     4141 gcagctggtc acatgaacag cctcattctg caactgccca aaatagctta taatcttttt
     4201 cagatatgcg tcatcacaac cattcttatc attaatatta aatacaagat ctttcagacg
     4261 ggaaatatat tcctgagatt tatcatcaag tgaaacacta taacgagaca atgttccttc
     4321 tgcactgttt tgaaacttat aaaacatttc cagtaatttt tttgcataag gcgttgtatc
     4381 aaagctggta aagtcataaa gatattgcgc agcgtaacca tacaaaccat gaatccagag
     4441 tccttctctc accaacactc tgagataata tgcaaggctt tgaagtatct tcgtctttcg
     4501 ttgtatatca agtaagacac ttaatgaata aaatgcatta tagaatttat caatattcct
     4561 gagcactttc tccagttcat ccttatccag cttcttctcc cggtaatcct ttttatattc
     4621 atttttgata gccatgatat attgttgcat caggctcttg cttttcccgt tatccttccg
     4681 tatagttcct gtatatttcc ccagagcata cagctttcca ttttcatgaa aatcttcctt
     4741 gttgaatccc agttcagcca gatattcccg aagataacgt ttaacagcag cttcatcgct
     4801 ttctgtatca gcgtgatcaa gacaaggaca atacgatttc tgtaaaaaat tataaatatg
     4861 ataataaaaa gcagagtcat tctctggtct gtatgtatta tacatatcta caacaagaaa
     4921 aataacccca aaagtgcgca gacaatagct gcgaaggaat tcaggtttta gtgtggaatg
     4981 cactggataa gaattcatca tgtaataaaa atgttcatat cgggcatata tgatccgccg
     5041 ttgctcctga ctgaacaccc cttcagcatc aataatacga ctgatctcat tataacgttg
     5101 atcccagaat ttttgatgca tttcaaaatt ggtactcttc cgcttgtgtg ccggattctc
     5161 gccttcctta aacaacccca aaacagtaca aatatcatac aacatatttg taaatgtgcg
     5221 catatgctgt gctctccggt tggtttgcat tttatcgcag aggacattct gagcttaacg
     5281 tatcaaactg acacaacgac atgcaatgca tatggagaac aatatgtcaa atcaaaccgg
     5341 acagctttac acgccttcac gcatcctgcg tatggctgaa cttacccgac tgctgggtat
     5401 atcaagatct actatttacg tgaaaataaa tccggcctcg aaataccacg accctacctt
     5461 tcccaaacca ataaggcttg gtagtgcatc cgtaggctgg cgggcatctg ccatcgatga
     5521 gtggctgatg ttacatacgg caccagcctg gtctgaacct gaaaacaaac taaaggaact
     5581 taatgatgac gacaaaacta tcactccctg aacttgacac aactaaactg cctgacaggt
     5641 gccaggcatt acttgatgaa atgcatgaag aaacaggaat acgccgtgaa atacttctgt
     5701 ctgttatgct gacggtcatg gctgcctcgg tccaggatac gcatgaagtt gaactttccg
     5761 gagggcaacg taccagcctt cagatattta tgtgtctttc atcagcatca ggcagtggaa
     5821 aaacctctgc ctgcgcaaaa ttaatcgctc ctgttcatga aacagaagaa gaactacatc
     5881 aggcatatat cgacgataaa aaaaactatg accgaatgat ggaaatgtgg acaacagata
     5941 aaaaaatcct ggagcggaga tataaaaagg aaattgaaag atccccggaa aatgctgctg
     6001 ctgcacgagc agcacttgaa aagtgtatag caaataaacc cgaacctccc gtacaacagg
     6061 ttctcatcgt gaatgacgcg acaccagaag ggatagcttt gaaactcagc cagtcaccct
     6121 ctcttctgtt gctgtctgat gaaggaggta ctattctgga taagcgtttc gaacgcaaat
     6181 cggcactgta caacacatta tggagtgggc aaccagtcaa cgttgaacgg gcatccagac
     6241 ccgggttccg gctgaatgat gtccgtctca ctatacttat actgactcaa ccggtaatat
     6301 ttaataagtt ttttactctc actggcgatc agatccgggg caatggcttt ctggcccggt
     6361 tgctattttg cgaacccgga gcaattaaaa taatgacgac agaaccgcat actgccactc
     6421 ctgttgtgac gcaacaatgt gcaagctgtt ttcgtaagaa aagttttggc agtcagatca
     6481 gagactctct gagagcatcc agagaaagac gtgcaaaaag taaagagcgc atctgcatga
     6541 cattatcaaa tgcagcatcc cgcgcactgg agcgctttca caaagaaaat atggatactg
     6601 tcagacagaa tcctcatatg ggtacatttg aagacattat tgtcagaaaa agagagcagg
     6661 ttgtcaggat agcagcactt ctggaactgg agaaagatcc agactcgaca gtaataacgc
     6721 tggaaagtac taatagcgcc atttatctca ttgaatttta tttcaagcat cttatatata
     6781 aacttgaatc ccttcgggaa atatccccgg ctgagaaact tgataaatgg cttcagaaaa
     6841 gaattatcac aacagcaggt tacatattcc agaaaagtta tattttacaa tatgccccat
     6901 atgctctcag gaaaaaatgt gttctggatg aagcacttga catactggcg gaacaaagga
     6961 aaataaggat tgatgataac ctggttgttt atataggaaa cacgattaca ccatccgaac
     7021 tggcaaaaaa actaaatatt cctgcattcg atgccggagt tttcatttgt gatcatcaaa
     7081 acatactgaa gtatcaccga aacagactat agatactatc ggacaaaaaa taaattatca
     7141 caagtgtatt attcgttata ataatgaata atcgttatct gagtgcaatg ccaggttttc
     7201 attacgaact ggcattgcat ttatttttca aaaaagaaag cagtaaccat ttggctactc
     7261 tgagtatgcc agtccatgaa actaagcgaa ttccttagtg tttttagcaa gttgagacat
     7321 cacataatcc gcccatgcct gcatcatcgg tacacgctgc tccagtagat cagtacgatg
     7381 atatgccgcc tcaaccttat ttttcagcgt atgagcgagc gccctttccg ccagatcccg
     7441 cgaatacccc tgttcactac accagtccct gaatgttgag cgaaaaccat gtgccgtggc
     7501 aactcgcccc ggaatgtcac tgacggcttt ctttttacgc agaaaacttg tcaacaccat
     7561 atcggaaagg atctgctgct ttctgggtga agggaacacc agttcatcat gcaggccacg
     7621 tatattttcc agaatgtaaa tagcctgccg ggataaagga acacgatgct gtagcctagc
     7681 tttcattctt tctgcaggta tagtccatac ccgcttatga aaatcaattt cagcccagcg
     7741 cattcccctg gcttcgcccg agcgagttgc tgtaagtatc accattaata acagtgcgcg
     7801 ggtaacatta taaggttcat cactatacac actggtcgcc acaaaaagcg gtaactgcct
     7861 ccagggcatt gcgggttggt gttcatcacg tcctcttgtc tgctgaggaa gcaaatggtc
     7921 aaccacatca acaggatttg ccacacaaaa accgtgcgcc catccccact gcataacaac
     7981 atgaatgcgc tgtttaaccc ggcttgccgt ttctgacaag gttaaccaga ctggacgcag
     8041 tgtttctgcc acgtccgcag ccgtaatcga atccagcgtt tttgctccca gttgaggaaa
     8101 cgcgtaattc tcaagcgtcg ataaccactg ccttacatgc tttggattct cccatccagg
     8161 agacagttct gcatgtacac gcctggctgc atcggcaaat gttgggataa cgactttctc
     8221 agattcagcc tttttaatct ccagaggatc atcacctgca gcaagttgct ctcgcattat
     8281 ccgggcagta cgtgcagctt cagcaatact gacctctggg taagttccca atccagcatt
     8341 acgtcttttt tgtgtcaccg gacttacata acgaaaaacc catttccccc gcccctttac
     8401 tgaagaagga tgaagggtca gtccggtaat tcccccatgg ggcaatggtt tgtcatcagg
     8461 tttgatatgt cttgctttcg tatccgtcaa tactgccata cgctaattcc cgtcactctg
     8521 gtatgccatc cagtatgcca ttagtccctc gcttcactaa gaatacatta gataacatcg
     8581 gacaacaaaa agtgtaatac attgtttata aagaaattat ataacacccc tggattaagt
     8641 cagatttatt tcaggcggtc cccctcaccg ccatatttaa agaagagcgc gtacgaaagt
     8701 acgggctttt ttttcgtata ttgtacacac cgggtgcccc cgcccacgtc tgttagggcg
     8761 aggggaaatt gtgcacatca actgtctggt tactcagtca acaatcaaaa tgcgcaagat
     8821 agtacagaac atatatgcgt tatctggtca cttcctgtaa cttcctcact tattaaaatg
     8881 cccccaacgg aaatacatgt cacatgccat cataaaatcg ttatgccaga ggaatcgaca
     8941 atggaaaaaa tagtaaatta agcacggtac atagcagagt aaaaaatagg cttcaaacca
     9001 aatccgccat tccacaagcg ttgccaatac cgacaccagc gtaatccaca tcatttcaat
     9061 aaccagcaat atgaataacc gaagccaaag cggcttggca aaaaaggtcg acaacgtatt
     9121 ttcactggag aaaaacagat cgatttcctg caaaaaaaac ctgcccagaa aagcgtaccg
     9181 cttggagaag cgcagaaaaa acagattaat cgccaacact acgccacaaa gaatgagcaa
     9241 tgcaattatc gatttaaacg ttaaccactg cccctctgtc acaaaagaat cactgttacg
     9301 caacgggctg atagtaagca ggttaacaac cgttgagatg cacaccgaaa gcagcacata
     9361 gcgagtcaga cttttatatt ttactttttt cgctactgaa atagcgacct gcttgacata
     9421 actggtgcgg atcatccgaa acagcagaaa gatgaccaac ggcccaagta acgaaatcag
     9481 gaacagcgca tataacgagg gtagaagctg gtatagcccc caactcattg cactcaacat
     9541 gattaacgac agaaatcctg ccaccagagg ttgtgtaaga aataattcta ttttgtggtt
     9601 ctgctttttc ttaaaccaca aaaaatcgat attcttcgtg acgcatttat aagcccagta
     9661 cgccttgaga tcaaaaataa aattatacag caccagggcc agaatcccac taactacacc
     9721 aatatcctct gatggaaaag taaaccctgc atttttccaa taaaagccta tcagaataag
     9781 tccaatagca taaatgtata tcagatttcg tttatcctga aagcgaataa aagttaaata
     9841 atcaggaatc atttgaatac ctttgcatca ctaaatgctt tacgacaatg cataactcgt
     9901 tgctgatcca cgccagcaaa atcatttaac atattcccca aatagggctt attacttatt
     9961 tc
//
LOCUS       DAJTNM010000133         9955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTNM010000133 DAJTNM010000000
VERSION     DAJTNM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.133"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO172_003469"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO172_003469"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5513515.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO172_003470"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO172_003470"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5513516.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="OO172_003471"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="OO172_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="OO172_003472"
     CDS             3456..5216
                     /locus_tag="OO172_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5513517.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="OO172_003473"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="OO172_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5513518.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="OO172_003474"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="OO172_003474"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5513519.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="OO172_003475"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="OO172_003475"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5513520.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2555 a         2495 c         2373 g         2532 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acatcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagagc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccagg
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 ctttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcaacgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttggat aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caatctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgta ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggca accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt acctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatgggtcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgctttttt attccgcgct tcgttctgct acagcgtttt taacacatct tatagtgcat
     9841 ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc
     9901 catcgaatac cactgtatta cacaagaaag tgacatcggc atcttcaata ccaag
//
LOCUS       DAJTNM010000134         9708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTNM010000134 DAJTNM010000000
VERSION     DAJTNM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.134"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(329..673)
                     /locus_tag="OO172_003476"
     CDS             complement(329..673)
                     /locus_tag="OO172_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5513521.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="OO172_003477"
     CDS             805..2715
                     /locus_tag="OO172_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5513522.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQLGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="OO172_003478"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="OO172_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5513523.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="OO172_003479"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="OO172_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5513524.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRRQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="OO172_003480"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="OO172_003480"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5513525.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="OO172_003481"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="OO172_003481"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5513526.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNARQLRTRQLACLQLLAD
                     WRLRKARECDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="OO172_003482"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="OO172_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5513527.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="OO172_003483"
                     /pseudo
     CDS             complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="OO172_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(9210..>9708)
                     /locus_tag="OO172_003484"
     CDS             complement(9210..>9708)
                     /locus_tag="OO172_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5513528.1"
                     /translation="FAAKCGLSDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQS
                     QSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPE
                     IFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQD
                     VKKAV"
BASE COUNT         2370 a         2368 c         2559 g         2411 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtagcagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc taggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg gtcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 agacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgatgagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgccaggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagtg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaactgca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 cggtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaac
//
LOCUS       DAJTNM010000135         9669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTNM010000135 DAJTNM010000000
VERSION     DAJTNM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.135"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            373..1491
                     /locus_tag="OO172_003485"
                     /pseudo
     CDS             373..1491
                     /locus_tag="OO172_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
     gene            1557..1805
                     /locus_tag="OO172_003486"
     CDS             1557..1805
                     /locus_tag="OO172_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5513529.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLSAIEYFVLRFIWRRWFSLAD"
     gene            1870..2238
                     /locus_tag="OO172_003487"
     CDS             1870..2238
                     /locus_tag="OO172_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5513530.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPRYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2332..2985
                     /gene="nfsB"
                     /locus_tag="OO172_003488"
     CDS             2332..2985
                     /gene="nfsB"
                     /locus_tag="OO172_003488"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5513531.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3093..4340
                     /gene="ybdG"
                     /locus_tag="OO172_003489"
     CDS             3093..4340
                     /gene="ybdG"
                     /locus_tag="OO172_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5513532.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4408..5784)
                     /gene="pheP"
                     /locus_tag="OO172_003490"
     CDS             complement(4408..5784)
                     /gene="pheP"
                     /locus_tag="OO172_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5513533.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5885..9020)
                     /gene="cusA"
                     /locus_tag="OO172_003491"
                     /pseudo
     CDS             complement(5885..9020)
                     /gene="cusA"
                     /locus_tag="OO172_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9032..9573)
                     /locus_tag="OO172_003492"
                     /pseudo
     CDS             complement(9032..9573)
                     /locus_tag="OO172_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT         2374 a         2685 c         2334 g         2276 t
ORIGIN      
        1 atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg gaaagcttca
       61 ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat acagcaaatc
      121 ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt tttttctgca
      181 tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg ccacattgaa
      241 aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa agaaacattc
      301 cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa aacgtcaact
      361 taagagaaac ctatgccatt acccgatttt catttttctg aaccttttac cctcggtatt
      421 gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga ctcttcaatg
      481 ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga tatcaccgaa
      541 agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc cgggcagttt
      601 tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga aatttgcggc
      661 ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa cgaacgctat
      721 caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt tggtcagcat
      781 gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg cttgtcacgg
      841 tttgtgccgc attttattgc cctttccgcc gcgtcgccat atatgcaggg aacggatacg
      901 cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg cccgatgccg
      961 tgggtcagta actagcaaca atttgaagcc ctgtttcgct gtctgagtta caccacgatg
     1021 atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt tggcacggtg
     1081 gaggttcggg tgatggatac cccgttaacc cttagccacg cggtgaatat ggcgggatta
     1141 attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca ggagaaagat
     1201 tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga aggcgtcatt
     1261 accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg attgctggaa
     1321 aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc actgcatcgc
     1381 caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc cgatggcggc
     1441 tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta agccgggaat
     1501 tgccttacag aagaatatcc ctgacaatgg tgttttaact tatatttaac aatgttatga
     1561 aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct ttcctctctt
     1621 gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg gtgaccatgt
     1681 tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg tttttagtgc
     1741 tgagcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc tcgctggcgg
     1801 attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc ttcgcctaaa
     1861 ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc tcttgagtta
     1921 ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa aattggcggc
     1981 aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct gaagtccgat
     2041 ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccaag gtatcacatg
     2101 aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc gttacttcgc
     2161 gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa
     2221 agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta ccatttctcg
     2281 ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt tatggatatc
     2341 atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa aaaacttacc
     2401 ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag caccaactcc
     2461 cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc
     2521 gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca cgtcgtggtg
     2581 ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga ccaggaagat
     2641 gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg tcgcaagttc
     2701 ttcgccgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc aaaacaggtt
     2761 tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga cgcggtgccc
     2821 atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga gaaaggctac
     2881 accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa cgctacgctg
     2941 ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc tcttgccggg
     3001 catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt ttcagccgtc
     3061 atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc caggttgaag
     3121 atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc ggtattattt
     3181 ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg accttcgaaa
     3241 aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat aaactcttcc
     3301 accgtttagc ttttaccctg cagggaatta tcgtcaatat tcaggcggta ttctggctgc
     3361 aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg atcatgatgt
     3421 atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg cagaaattcc
     3481 cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg atcggcgcaa
     3541 tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg attctgatca
     3601 gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg attcttggtc
     3661 tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac tggctggaaa
     3721 tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc
     3781 gtaactggga caataccatt accactattc ccacctggtc tctggtttct gactccttta
     3841 aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt atcagtattg
     3901 atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat aaagcgcatt
     3961 tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat cgccagcaag
     4021 gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct
     4081 atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc ttaatggtac
     4141 gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt actaacaccg
     4201 tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata tttgcgattg
     4261 tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt cgctctctgg
     4321 cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga gagcggacat
     4381 cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa atgccacaaa
     4441 caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat ccatcgtgca
     4501 catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga acggatagag
     4561 cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa aacgcagatg
     4621 cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg tcatcagcag
     4681 accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag tgatcgctcc
     4741 ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca aaaacttcgg
     4801 cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa ccccgctgtt
     4861 atacaccgac agcgatgcca ccagaatgac gaagttcagc gcagaagcta ccacattgct
     4921 gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt ccacccacgg
     4981 atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc gatacaccac
     5041 ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg ctgtaatccc
     5101 aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa tcagcccatt
     5161 ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg ctttctcgcc
     5221 gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga tcgccagcac
     5281 tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca ccaggttaac
     5341 ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat ccgggaacca
     5401 gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca cgaacattac
     5461 ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat aggcaaagtg
     5521 ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc gcataatcag
     5581 gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg ccatctgaat
     5641 cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg caatcagttg
     5701 aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg cagtatcttc
     5761 cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt tgaggggcac
     5821 cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa cgaggtatcc
     5881 tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc gccgggataa
     5941 taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggggcg gcaatccggc
     6001 tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg gcgataatca
     6061 ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac aacgcctcat
     6121 ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc tctatggcgt
     6181 gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca agggcaataa
     6241 agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac cagatgccgc
     6301 ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc cgacggaatg
     6361 ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt ttcagcttat
     6421 ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg cccggtttta
     6481 actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc atgtcacgat
     6541 cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc agcatcgacg
     6601 gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc ttcatcggcg
     6661 tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc ggataacgca
     6721 gattaattgg ataacgggca atcccttcca ccctttcgcc aaccatcgcc ccgcccaccg
     6781 cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg gctttttcac
     6841 ggttaatctc aacgttgata tagcgcccac cttccagccg ctcggcaagc gcagaagcta
     6901 cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg atatccgcca
     6961 gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag agcatatcga
     7021 tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc acggtgttat
     7081 ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc tgtggcttaa
     7141 gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt tccgctttcc
     7201 cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg tcggtttttt
     7261 gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc atatacaaca
     7321 agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc ggccagagaa
     7381 ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc agtactttca
     7441 gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg ctttccggcg
     7501 gaattttgcc acggatccag taacccatca ggatagggat cactacgatc gccagcagcg
     7561 ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga cgcccttcct
     7621 gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga ctgataaaca
     7681 gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt ttattatcca
     7741 gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca ttctcgatca
     7801 tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc agcgacataa
     7861 tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac cccagcggca
     7921 acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgcg cagactaccg
     7981 ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc cggtcaatga
     8041 gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta cttttcagcg
     8101 tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg gatcgcagga
     8161 tcaccacccc gcccgccact tcgccttcgc cgtttaatgc cccggcgcat ctccgggcca
     8221 atctggacct tcgcaacatc gcgcagataa acgggcacgc cattttcact ggcttttaaa
     8281 acgatgtgat taaagtcgtc gagcgtttgt agatagccgc tggcgcgcac catatattct
     8341 gcttccgcca gttcgattga cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt
     8401 tttacttcgg cgagactgat gccatactgc gccaggcgct gaggatcgat aaccacctga
     8461 tactctttca ccacaccgcc taccgacgcc acttccgcaa cgtcaggaat ggttttcagc
     8521 tcatatttga gaaaccagtc ctgcaatgag cgtaaatcgg caagatcgtg cttaccgctg
     8581 cgatccacca gtgcatattc atagatccag ccaacacccg tggcatctgg tcccagctcg
     8641 gcgctgactc ccgcaggcag cttaccctgt acctggttga ggtactccag cacccgcgaa
     8701 cgcgcccagt acggatcggt gccatcttcg aaaatgacat acacataaga gtcgccaaac
     8761 tgcgagaaac cacgcacagt cttcgcgcca ggcaccgaca acatggtggt ggttagcgga
     8821 taagtcacct gattttcaac gatttgcggt gcctggccgg gatagctggt tttaataatc
     8881 acctgcacat cggagagatc cggcaacgca tccactggcg tattaatgat ggtccaggtg
     8941 ccccagatgc tcagaaacaa cgcgcccatc agcaccaaaa aacggttcgc caccgagcga
     9001 cgaataatcc attcaatcat tggttattcc ctcaatgcgc atgggtagca ctttcagagc
     9061 gcatccgctc cagtgcgcca gaaatattgg cttcggaatc aatcaggaac aggccgccgg
     9121 aaaccacctt ttcaccttcc gccagaccag agcgtaatgc ggtgacgcct tgcgatgcct
     9181 ggaaaacagc aacgcgtttc ggtacaaagc gcccgtcggc atcaacggta atcacccgct
     9241 gttcgttgcc ggtatcaatc agcgcctcgt cggcgttgtc gacttccaga cgcagctgca
     9301 gcgtgcgggt cgcggcatcc acgccaggta gcagcgtcca tttgcggatg gtgagtgttt
     9361 tatccggtcg cgccggaacg gtgagggtaa actgcgaggc atctttcacc agccaggcga
     9421 tggactccgg gatcgcagca gtgacccaca ccgggtccat accctgaatt cgcgattacg
     9481 cgcaataccc ctgagaaccg ccatgaacaa gggtttcatg atttgtgatt ttagtttagt
     9541 acgcaatttg gtacacaaca ctattttcac ttcagcgggt agtcatcaaa ctcaccataa
     9601 cgagcatcgt tgatgatgta tgtgatcacc acaaatatca catctggact agttccgtct
     9661 tcatgcgca
//
LOCUS       DAJTNM010000136         9386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTNM010000136 DAJTNM010000000
VERSION     DAJTNM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.136"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..569
                     /locus_tag="OO172_003493"
     CDS             <1..569
                     /locus_tag="OO172_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5513534.1"
                     /translation="NAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAMASQR
                     TKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGAAKMLFSHA
                     QKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDF
                     SHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            587..1225
                     /locus_tag="OO172_003494"
                     /pseudo
     CDS             587..1225
                     /locus_tag="OO172_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1516..2244
                     /gene="mpaA"
                     /locus_tag="OO172_003495"
     CDS             1516..2244
                     /gene="mpaA"
                     /locus_tag="OO172_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5513535.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2219..3184)
                     /gene="ycjG"
                     /locus_tag="OO172_003496"
     CDS             complement(2219..3184)
                     /gene="ycjG"
                     /locus_tag="OO172_003496"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5513536.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3303..3809
                     /gene="tpx"
                     /locus_tag="OO172_003497"
     CDS             3303..3809
                     /gene="tpx"
                     /locus_tag="OO172_003497"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5513537.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3853..5394)
                     /gene="tyrR"
                     /locus_tag="OO172_003498"
     CDS             complement(3853..5394)
                     /gene="tyrR"
                     /locus_tag="OO172_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5513538.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5542..6603)
                     /locus_tag="OO172_003499"
     CDS             complement(5542..6603)
                     /locus_tag="OO172_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5513539.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6600..7997)
                     /locus_tag="OO172_003500"
     CDS             complement(6600..7997)
                     /locus_tag="OO172_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5513540.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8153..9151
                     /locus_tag="OO172_003501"
     CDS             8153..9151
                     /locus_tag="OO172_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5513541.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9262..>9386)
                     /locus_tag="OO172_003502"
     CDS             complement(9262..>9386)
                     /locus_tag="OO172_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpG family monomeric porin"
                     /protein_id="HCR5513542.1"
                     /translation="GYDFQNGLSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         2373 a         2496 c         2257 g         2260 t
ORIGIN      
        1 ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg ggtgttaata
       61 tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta ttgaatgcaa
      121 tggcgtcaca acgaacaaaa gaggctcgtg gtggagaatt acagatcaac gaattacttc
      181 ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt tatgaagcaa
      241 ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcggcaaaa atgttgttct
      301 cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg ttactgaccc
      361 agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat cgtgatggac
      421 tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg ttacccgatt
      481 tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg gcaaaagtta
      541 ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga aaaatgtact
      601 gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg cagataaaca
      661 aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac cctatccaac
      721 taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc aggccatgca
      781 gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc aggcaaattc
      841 agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc tttccctcgg
      901 tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta ttggtgaacc
      961 cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg aatatacaat
     1021 tcttcgccct gcctagctga ctgatgaaga tattattgat tatgagctta cctctcgcaa
     1081 tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa ttacagatat
     1141 catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac ccgggactga
     1201 tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac tgtactatgt
     1261 gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga ttaacatgtg
     1321 tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc taatatctgc
     1381 acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg atgacatagc
     1441 gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt aatacttcct
     1501 gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc atttccgccc
     1561 ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt tcctgcccct
     1621 gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga aaactcttcg
     1681 gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg ccatcatgtg
     1741 gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc taatggtgtg
     1801 gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt ttatcgctgg
     1861 aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc tggctccgaa
     1921 cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg ggtggtctca
     1981 ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg cgaatggctg
     2041 gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc tggctctttc
     2101 ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc gcccatctcc
     2161 tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg ctggcatcct
     2221 aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc gccagccagg
     2281 tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg gcgctaatgg
     2341 cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca cgcgcttcag
     2401 tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg ttaaccatct
     2461 catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca tcagcacaaa
     2521 tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga agcggttgtt
     2581 caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac ccttctgcac
     2641 gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg cgaattgcca
     2701 ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc gcgcctgcct
     2761 gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca accgtctgtg
     2821 cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat tgctgctgtt
     2881 ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg ccggcaggga
     2941 gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg acgctcataa
     3001 tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg cattcgccgg
     3061 tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca ctgcggcttc
     3121 cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc ttaacgattc
     3181 tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag cgtttgctgt
     3241 gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca ggaaagataa
     3301 tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat tccatcccgc
     3361 aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct gacgtcaccc
     3421 tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt gataccggtg
     3481 tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac aacaccgttg
     3541 tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc gcagaaggtc
     3601 tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa gcctacggtg
     3661 tggcaattgc tgatggccta ctgaaaggtc tggcagcgcg tgccgttgtg gttattgacg
     3721 aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag ccggattacg
     3781 aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt gcaccatcag
     3841 gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc gcaacttatt
     3901 ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc tgggataatt
     3961 gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt ccagcgaacc
     4021 ttccatcgca tcttcgccca cggctaccgt tgctgcgtca taatccggca acaaaatatc
     4081 ctgtggacgc agctcataac cgtccagttg tgtcagcgcg cgatagatag cgttctttaa
     4141 ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt cagcggccag
     4201 tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct cagttaacgg
     4261 cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca cgttcagacg
     4321 ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat tcttctgcgt
     4381 agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc gacggaaagt
     4441 gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt cccctatttc
     4501 atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc cttccggagc
     4561 atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat tcagcgccag
     4621 gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa gatctttacc
     4681 tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt tctgcgcctg
     4741 ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg cgctgacgtc
     4801 ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca acatcaccac
     4861 cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg taatctccat
     4921 caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg gttcgctttc
     4981 cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt tgcgcaggcg
     5041 atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca ctttgctttt
     5101 catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca acgccagatg
     5161 ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg caatacggcg
     5221 tatttcggcc atcaaactgc tgaaactctc aaactccagt tcagcaaaat tgaggtagat
     5281 tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca cgagtagatc
     5341 gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac gcataggaac
     5401 cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat agcaggaagg
     5461 tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg taaagatatc
     5521 cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt gcagagtttc
     5581 tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt catcgtcaat
     5641 ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg tcagaagtcc
     5701 agctccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt gcgacatcca
     5761 gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat tcagcaatac
     5821 cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca gcgtggcaat
     5881 gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca acgccagcgg
     5941 gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc gccgcgcctg
     6001 ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac gatcgttctg
     6061 cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat caatacccgc
     6121 ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc tatgcaataa
     6181 atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc gccgccactc
     6241 tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac atccacccag
     6301 cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct gcccgacaac
     6361 gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc tgcgtttcgg
     6421 acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg cttcgtcgag
     6481 cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg ccctgaattt
     6541 tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta acggttcggt
     6601 catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat atgcggcagc
     6661 ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc ttgcttgtcc
     6721 cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt gagaggcgca
     6781 ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac gtcaataatg
     6841 ccgctggtgg tcgtctgcac cgaggccagc cccaggcaat ccatactaat cctttcaaac
     6901 gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat attggcgtgc
     6961 tgatcgaccg ttacatggtc cgctttcgtc gcggcaaaca gcagtttgtc gataaccggc
     7021 gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat cagctgcgtc
     7081 agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg caggcaatcc
     7141 accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc cttcaccact
     7201 ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg cttatcggcc
     7261 tgcgccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa ttgcagcgcg
     7321 ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa gtgcagcccc
     7381 tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc cagccggttt
     7441 tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt cgccgaccat
     7501 tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa gtaatcctgc
     7561 gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc cagatacagc
     7621 gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttaaaacg taacgccagg
     7681 cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc atataactgc
     7741 gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg aatgcgtttt
     7801 acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc tccggcatga
     7861 atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct gcggctaagt
     7921 ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc attgagttca
     7981 tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa aaatcattcg
     8041 taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt gtcatcgacg
     8101 ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac ctatgtcccc
     8161 tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat cacgcgtgct
     8221 gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg ccattgaaga
     8281 attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt ttgatgccat
     8341 tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt tctctgaagt
     8401 tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat tacaaacgtc
     8461 gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga aaatgattaa
     8521 aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac tcgataaata
     8581 cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg tttattctgt
     8641 cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg aaagtgggca
     8701 tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg atcgcgttaa
     8761 tggctataag aaaagtctgt ccgcgcataa tattgcagtg cgtgataaat ggattgttga
     8821 cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa gtcagtcacc
     8881 gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt atcgtgcggc
     8941 ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata gtaatgaaac
     9001 gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc aggagttagg
     9061 tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac cacagaatat
     9121 taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg aatgccggat
     9181 gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta ctcgcgccgc
     9241 atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg cataatggaa
     9301 tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca gcgaaacgga
     9361 aagtccgttc tggaaatcat aaccgt
//
LOCUS       DAJTNM010000137         9241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTNM010000137 DAJTNM010000000
VERSION     DAJTNM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.137"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO172_003503"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO172_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5513543.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO172_003504"
     CDS             1828..3147
                     /locus_tag="OO172_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5513544.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3433..3474
                     /locus_tag="OO172_003505"
     CDS             3433..3474
                     /locus_tag="OO172_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513545.1"
                     /translation="MVQLVDLARCVSF"
     gene            3512..4657
                     /gene="dosC"
                     /locus_tag="OO172_003506"
     CDS             3512..4657
                     /gene="dosC"
                     /locus_tag="OO172_003506"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5513546.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4582..5199
                     /locus_tag="OO172_003507"
                     /pseudo
     CDS             4582..5199
                     /locus_tag="OO172_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5457..5789
                     /gene="ddpX"
                     /locus_tag="OO172_003508"
                     /pseudo
     CDS             5457..5789
                     /gene="ddpX"
                     /locus_tag="OO172_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5803..6699
                     /locus_tag="OO172_003509"
     CDS             5803..6699
                     /locus_tag="OO172_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5513547.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6696..7682
                     /locus_tag="OO172_003510"
     CDS             6696..7682
                     /locus_tag="OO172_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5513548.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO172_003511"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO172_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO172_003512"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO172_003512"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5513549.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2256 a         2256 c         2321 g         2408 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acacttagtt aaacagctat aaatccctaa taaaaatgta tgaatatgaa
     3301 aaaccatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     3361 aacagtagcg tagattgaaa ctgtcttaag tcgcttattt cttttcagta tatcttcata
     3421 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gcgtttcgtt ttgattccgt
     3481 ctgatttccc agccttataa gggtgtgaat tatggagatg tattttaaaa gaatgaaaga
     3541 tgagtggacc ggacttgtcg aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat
     3601 tgcccttgcg catgctcatt atctgagtat cgagttttat cgaattgtcc gcatcgaccc
     3661 gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga
     3721 acgctggatt attaacgtgc tttctgccca ggttgacgat gtcgaaaggc taatacaaat
     3781 ccagcatacc gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat
     3841 ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg attattccgc
     3901 cgcagaaaaa cttcaggtct accatttctc gattaacagt attgatatcg cgatggaagt
     3961 gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg
     4021 tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact
     4081 ttcatgggaa atagatatta tctataaagt cctgctggat tctgatttag gcagcagttt
     4141 gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag
     4201 cggcattgcc gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa
     4261 tcaaaccatg cgtaacacca ggattttgaa taacagaagt ttgcgggtga aatttctatt
     4321 acagataaga aataccgtat cgcagattat taccttgctg cgtgaattgt ttgaagaagt
     4381 atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc gtttcctgcc
     4441 gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct
     4501 gattattgac gttgataaat tcaaagagat caacgatacg tggggccata acactggcga
     4561 tgaaattctg cgtaaagtct cattcctctc gcagaagaga ttggtgaaat cgaaaatatt
     4621 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
     4681 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
     4741 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtggaa
     4801 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
     4861 cgtgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc cggattatcc
     4921 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagctttgt cgatcgttgt
     4981 ctgacggaaa aacgcatcct ggccttactt gaagccatta ccagcattgg gcaaagcctc
     5041 aatttaaccg tcgtggcgga aggggtcgaa accaaagagc aatttgagat gctacgtaag
     5101 atccactgtc gcgttattca gggatatttc ttttcccgcc cccttcccgc cgaagaaatt
     5161 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact
     5221 cgcgggtttt cctttaaggt gacactgtca cttaaatact gtgatttcag ctacgattcc
     5281 gggagattcc ttccttaact ttccctcttg caccaaaact gcactacgct gcacaaatgt
     5341 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
     5401 atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa ccgtttatgt
     5461 cggataccac cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga
     5521 aatatgcctg cgttgataac atcacaggta aagctattta tcagcaagcg cgttgtctgc
     5581 tacataaaga tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctgtcaggct
     5641 tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag
     5701 cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca
     5761 cggcgctcga cctgacgctt cgtgatgacg tggaggtggt gaatgatgct aagcgaggaa
     5821 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
     5881 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gcgactgatg
     5941 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
     6001 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttt
     6061 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtggt ggtcgccatt
     6121 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
     6181 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
     6241 gcactggctg ccgctctcgg gccgagttta tttaacgcca tgctggcgat tgctattgtg
     6301 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
     6361 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
     6421 ttacgtaact ccctaccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt
     6481 ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg
     6541 ggcgcgatgg tagcgaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt
     6601 ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc
     6661 gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc
     6721 aacagctgca tttgagtttc cccggtttta acggtgatgt tcacgcgctc aacaatgtgt
     6781 ccttgcagat taaccgcggc gaaattgtcg gtctggtggg agaatccggc tcaggtaaat
     6841 cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg
     6901 ggcagatttc actgctggga gaagatgttc ttaacgctcg ggaaaaacag cttcgtcagt
     6961 ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctt aatccgacac
     7021 gtcgaatagg tcttcagatg atggacgtaa tccgccatca tcaaccaata agtcgtcggg
     7081 aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat gccgtggaag
     7141 ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc
     7201 tggcattctc ctgcgagccg caattgatta ttgccgacga accgacgacg gcgctggacg
     7261 tcacagtaca gttgcaggta ctgcgtttgc ttaaacataa agcacgcgcc agtggaactg
     7321 cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg
     7381 tgatgtatgc cggaagcgta attgaaagcg gcgggacggc agacgttatc catcatcccc
     7441 ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc
     7501 cattacccgc cattccaggg acggtaccaa acctcaccca tttgcctgac ggctgcgctt
     7561 ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggtaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttactgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gagctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgagctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 a
//
LOCUS       DAJTNM010000138         9160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTNM010000138 DAJTNM010000000
VERSION     DAJTNM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.138"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..924
                     /locus_tag="OO172_003513"
     CDS             <1..924
                     /locus_tag="OO172_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5513550.1"
                     /translation="LGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAP
                     PALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPVKPRP
                     QLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAI
                     PFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAV
                     AFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDT
                     THSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="OO172_003514"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="OO172_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5513551.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="OO172_003515"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="OO172_003515"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5513552.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="OO172_003516"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="OO172_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5513553.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="OO172_003517"
     CDS             4447..4806
                     /locus_tag="OO172_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5513554.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="OO172_003518"
     CDS             complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="OO172_003518"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5513555.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6060..6250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6315..8159
                     /gene="btuB"
                     /locus_tag="OO172_003519"
     CDS             6315..8159
                     /gene="btuB"
                     /locus_tag="OO172_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5513556.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALDHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8104..8961
                     /gene="murI"
                     /locus_tag="OO172_003520"
     CDS             8104..8961
                     /gene="murI"
                     /locus_tag="OO172_003520"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5513557.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2094 a         2219 c         2469 g         2378 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactggtat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcgc ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tctacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt gataggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tattttacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaatcaa cataatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggca cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atttgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcatcgcgc agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag
     5581 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
     5641 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag
     5701 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
     5761 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg
     5821 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
     5881 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
     5941 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
     6001 gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc
     6061 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg
     6121 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
     6181 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg
     6241 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
     6301 gtggatgctt tacaatgatt aaaaaagctt cgttgctgac ggcgtgttcc gtcacggcat
     6361 tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg
     6421 aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc
     6481 gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc
     6541 aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg
     6601 tgctggtgtt aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc
     6661 ttagccagtt ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg
     6721 ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac
     6781 ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta
     6841 cgcagcaaca actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc
     6901 atggttatga tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg
     6961 gttttttaag taaaacgctt tatggggcgc tggatcataa ctgtactgat gcctggagcg
     7021 gctttgttcg cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg
     7081 gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct
     7141 ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca
     7201 actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat
     7261 acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg
     7321 actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac
     7381 gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg
     7441 cagcacgcag cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg
     7501 gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac
     7561 caaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca
     7621 aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat
     7681 atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg
     7741 aagggaaagc gcggattaag ggcgtcgagg cgacagccaa ttttgatacc ggaccactga
     7801 cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt
     7861 tacgccgtgc taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact
     7921 ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt
     7981 atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca
     8041 cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag
     8101 tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa
     8161 ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag
     8221 atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg
     8281 tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg
     8341 caagaacgtt atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca
     8401 cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa
     8461 cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt
     8521 aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg
     8581 ctgggctcgg cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct
     8641 ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc
     8701 gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca
     8761 gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc ttggttgtta
     8821 gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg
     8881 acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa
     8941 aaactggcag ttttaggctg atttggttga atgttgcgcg gtcggaaaat tattttaaat
     9001 ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
     9061 caaacacgcc gccgggtcag cggggttctc ctgagaattc cggcagagaa agcaaaaata
     9121 aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc
//
LOCUS       DAJTNM010000139         9096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTNM010000139 DAJTNM010000000
VERSION     DAJTNM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.139"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(1..176)
                     /locus_tag="OO172_003521"
                     /pseudo
     CDS             complement(1..176)
                     /locus_tag="OO172_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            174..498
                     /locus_tag="OO172_003522"
                     /pseudo
     CDS             174..498
                     /locus_tag="OO172_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(528..3440)
                     /locus_tag="OO172_003523"
     CDS             complement(528..3440)
                     /locus_tag="OO172_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513558.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3443..3559
                     /locus_tag="OO172_003524"
     CDS             3443..3559
                     /locus_tag="OO172_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5513559.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3923..4765
                     /locus_tag="OO172_003525"
     CDS             3923..4765
                     /locus_tag="OO172_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5513560.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4768..5856
                     /gene="capU"
                     /locus_tag="OO172_003526"
     CDS             4768..5856
                     /gene="capU"
                     /locus_tag="OO172_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5513561.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNEPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5861..6811
                     /gene="virK"
                     /locus_tag="OO172_003527"
     CDS             5861..6811
                     /gene="virK"
                     /locus_tag="OO172_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5513562.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6876..7820
                     /gene="lpxM"
                     /locus_tag="OO172_003528"
                     /gene_synonym="msbB"
     CDS             6876..7820
                     /gene="lpxM"
                     /locus_tag="OO172_003528"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5513563.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(7974..9006)
                     /locus_tag="OO172_003529"
                     /pseudo
     CDS             complement(7974..9006)
                     /locus_tag="OO172_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2374 a         2090 c         2178 g         2454 t
ORIGIN      
        1 ttttttacca ccgggagttt gggcataacg gtgctttcag taagtacgac aggtactgtg
       61 gaggcacttc cgtaaagaag ggcatcaagc tcctgctccg acatacctgc aggcaaaggc
      121 catgaaagcc ctgatttccg gaagcgagcg aacagtccac aaactgttga acgtggtgtc
      181 agcggcggac agggataagc cctcgtcagc agttccgtca gcactgcgac agcgttcttt
      241 acccggtcaa aacagcgttg cggtgcacca cgcctgacgg atgaactgcg tgctcagggt
      301 taccacttta acgtaaaaac cgtggcggca agcctgcgcc gtcaggggct gagggcaaag
      361 gcctcccgga agttcagccc ggtcagttac cgcgcacatg gcctgcccgt gtcagaaaat
      421 ctgctggagc aggattttta cgccagtggt ccgaaccaga agtgggcggg agacatcacg
      481 tacttacgta cagatgaagt tcggttacat cctgtcagca cggaacctca cgctttttga
      541 gcggcacgac ggatcccccc cacccagtat ccgacagact tttcactgcg ttcgtaaaaa
      601 cccttcttcc ctcccagcat atccactaat tccggtgaca tatcctgcag ataagtctgc
      661 aaatcatgcc ccagatcttc cggtgactgc aactcataaa ccgcgcgcca cacatcaccc
      721 tgccttattt taagccccgg gttaagaata tactgtccgt ggttcagcct cagaaaaagc
      781 ttttttccat aaggattttt gctgttaact tcatgttgag agagctggct gctgatataa
      841 ctccttcgtt tgcggcgttc cggcagcaat gcttcaggaa gatgaaccac acactctgcc
      901 agtcgtgccg cactgaaggc ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac
      961 agcgcctgca tcaggatcac cagaaacatt gccatctgcc ggttgtccag tttgatgagg
     1021 cgtccttcca cctgcagtga cagactttca ggtttcagca gggtatacaa ctgcgcactt
     1081 ttctgttgcg cataacgggg cgtactgacc gccgctgaca gaacttgatg ataagccgcc
     1141 agcccgtcat taccggtcag caagggatct gcaccacgct ccagcagtaa ctgaaccagt
     1201 gcaatattac ctgcccgggc tgctgacatc agcggagtaa gattaaacgg cgaacggtga
     1261 tcaacaccat aacgttcaat atcccgcaac acggcaaccg ggttattcat tgagtatggc
     1321 cggaaatact tcagctccag taactgcaga gtcttcgttc tgggctgcct tgtgggctta
     1381 aaatcttccc ggtacagtgc attatacagc gggaacaggg aataaatcag actgtactcc
     1441 agcgcctgca gttgtatggt tttatcggct gtacctgcga ggatatgctg tttccactgt
     1501 cgtgcatcct ctgcggtgat aatctgccat ggcatctcag atgtctgcag gatccggcta
     1561 cggatggtgt ccgcctgctc ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc
     1621 cactcatcac ggctggaaat ctcttcctgc agattcagag gctcttcctg atgcgtgagc
     1681 gcaagcagac tccacagagg atgctccacc tgctgttcaa caagatacac attgtgtgta
     1741 gcacgggtga gcgcaacata cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga
     1801 tcctgcctgt cccgcggacg ggaataacgg gcctcgccct caagatcagc cggtgtaaga
     1861 ccctcgcaaa tatcatcaaa tgcctgccgg gcagcgctta ctatattgta aaggatcacc
     1921 gtttcgtatt ccagcccctt aacctcctga accgaaaaga ccagcggcgt actgaaccag
     1981 catcgcgcct gtgctttctg ttccggatgc atcacaatga ccgctacacg attcgaaagt
     2041 gaggttttgg cattcagttc ctgctttgtc tcttccctgt cgtccagcaa ccggatgctc
     2101 ccttctgcct gaccgcagct tctgacaaaa tggtgacttt cccgatcaat agcactaaag
     2161 cggacctgtt tgagtctcag caaccggtta gccagtgcag tgacgtggtg cccgttacgg
     2221 taattcgcct gcaggatatt aacggttgtg tcgtttccct gttgctgccg gaaaaagaga
     2281 cttttgagcg aactccatga gaaaaagctc ggatgaacta tctgattggc atcaccacag
     2341 agtaaaaact gaccgggatg acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc
     2401 atatcctgaa cttcatcaac aaaaatgaca tcataacggg gagttgccaa cgaaagatac
     2461 tgatgactga gcagattact gtcatactga tgcgactgtt tcagccatgc gatataacgt
     2521 tcaaacagga catagaccgt cgggcggtcc tccatgccat agagcgactg ccgtatcccc
     2581 agtgacagat acgcctcccg ggacagcggc ccattcccgg aggcaaccgc accgatcacg
     2641 cccctgaact cctcatacag ggtatgggca gcccccagtg cggcgatagc cctgttgcgg
     2701 ggcagccagt cgctgaacgc actgaaagtc acctccctgc cttccggaat gcgcagagtt
     2761 tccagaaatt ccgtcagcga aagaaagtca atattctgta cctcactccc ttcccctgaa
     2821 gcgtcataga gagtacgcgc tttttccacc agaaatgagg aaaggctgag atacagtata
     2881 tcaccagcag cctgcttcat tttttccagt accagcgccg tcttaccact tcccgccgag
     2941 ccaacaatca ccagcggtag cgggtgttcg tacagtgcct gctgatcatc atcaaaggac
     3001 agcattttat ccaggcggtg gaaacgccca tgagacggat tgatatacgt cagcgcttca
     3061 gtcgcgatat caacaggatc cggcactggt tgttgttgta agcgcccttc atcaatcacc
     3121 acgtttctgc gaagaaaacg tgaagtgtta taggcatgat tgcgaatata ttccagaaca
     3181 aggcagatag tttcaccgtg gtagcgatac agcgaaaaca gtaatcggtc ccggatactg
     3241 agccgggcgc ggtaaagatt ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg
     3301 gtggcaatag cctgtgcaaa ttttttataa cccgggatcg tgtcaggatt cagagtatga
     3361 taaagacaga ttttcatggc tgccggtttc cgtcgtgcaa aaaacatgct gattttacga
     3421 aggaatgcct tactgaacaa ccttgcccgg aatacccccc gaaatcagat ggaacaatca
     3481 gaaaaatgct ctgcagttat ggaataaaga aggtggggtg gtacgccggg agggcgatcc
     3541 gctggaaatg gcgcagtagc tgcaggcctg tcatgatgcc ggaatagaag ttcgtgtaca
     3601 ggttaatgaa agcgtcaccc cgtaaataaa ctctgcgctg aaattccttt ttttcattgg
     3661 agcacctgtg ttgttctgag gtgaacatat cacctctgtt caggtagcca aattcagtgt
     3721 gccacctcaa ggttcttagt gagtcgtttt taaaagatat gataatatat cattgtttat
     3781 gaattgttga tgtttaattt agtaaattat atcaaatatg ccatgggttt gctatgttat
     3841 tcatccagta tctggaaata aaatgcacca gctttattgg gttttccttt tgtctctgct
     3901 tactaggtgc cagaaacttt aaatgttaaa cgaagggggc attttgttca aagccaatca
     3961 tgttccggta ttaatgtatc atcatgtcag tcattgcccc ggactggtta cactttctcc
     4021 cgtgacattc cggaagcaaa taaaatggtt ggctgaaaac aactggaaaa cactgtcatc
     4081 agatgaactg gagttttttt atcggggcgg aaaactcccc cggaaaagcg ttatgttgac
     4141 atttgatgat ggataccttg acaactggtt tcaggtatat cctttattaa aagagtttaa
     4201 tctcaaagca catatttttt taattaccgg ttttattggg aatggtccgg ttcgtcactc
     4261 tcctggaaag gaatattctc atcgcgattg cgaacatcaa atagcgacag ggaatgctga
     4321 taatgttatg cttcgctggt ctgaagtaaa cgaaatgttg caatctggtc tggtcgaatt
     4381 tcatgttcat acgcatactc atacacgctg ggataaaaag ttcagttcac gagaagaaca
     4441 gtgtaagcat cttcggcagg acttgttgag cgggagagaa tatttgaaag aaatgacagg
     4501 aaaatgcagt aaacatttgt gttggccgga aggttattat aacaaggatt atattcaggt
     4561 cgctgaagaa cttggattct attatttata tacaacagaa agaagaatga atgctcctgc
     4621 taaaggaact acacggatag gaagaataag caccaaagaa cgggaaagtt gcgcatggct
     4681 gaaaagacga cttttttatt atacaacacc gtttttttca tcgttgctgg cctttcataa
     4741 ggggccaaga ttaccggatg attaataatg aatatactat ttacggaatc atcaccgaat
     4801 ataggtggtc aggaattaca ggctgttgct caaatgaagg ccctgaagaa aatggggcat
     4861 tcagttctgc ttgtctgcag ggaaaacagc aaaattgctt ttgaagccag taaattggga
     4921 attgatatca cattcgcgtt atttcgaaac agtcttcaca tccctactgc atggagatta
     4981 ctcggaatag ttcatggttt tcagcccaat gcaatcgttt gtcacagtgg gcatgatagc
     5041 aatattgttg gtttagtacg gttattcact aggaaacatc cattcagaat tatcaggcaa
     5101 aagacatatt tgacacgaaa aacaaaagtt ttttcaataa atcatttttg cgatgaggtg
     5161 attgttcccg gaacaagtat gaagacacat ctggagcagg aaggatgtcg aacccgggtt
     5221 actgttgtgc ctccaggctt tgatttccag aaattatacg ttgattcccg aaacagtttg
     5281 cctccaaatg ttctttcttg gctggcgtcc cgaaggggat gccccgttat tgctcaggta
     5341 ggaatgttgc gcccggaaaa agggcacgaa tttatgttga atttactgtt ccatttaaaa
     5401 atgaatggac gacagttctg ttggttgatt gtggggtctg gttcgcctga actgcgggag
     5461 catttacagt atcagattga cagtatgggc atgcatgatg atgtttttat tgctgacaat
     5521 gtttttcctg ccgcccccgt atatcgggtt gccagtctgg tggttctgcc ttcagaaaac
     5581 gaatcgtttg gtatggtact ggcagaagca tcggcatttt ctgtgcctgt actggccagt
     5641 cagattggtg gaatccctga tgttattcag aacaaccaga ccgggacatt gttaccagca
     5701 ggtaataagc acgcatggat gtgcgccctg aatgattttt ttaatgaacc tgggcgtttt
     5761 tatcagatgg ctcgccaggc aaaacaggat atagaagagc ggtttgatat taataaaact
     5821 gcgttaaaaa tactcacatt agcgaagcac aagtaacaat atgttttctg taagtaactt
     5881 atcatttatc ggtttcctta aaaggattgt tttttcctca gattcactcc cggggaagtg
     5941 ggaacacaga aaattccggt tcatgtacat tttgcgatgt gctataaatc cggttgccag
     6001 tattcgatat tattacgaac tgcgttcctt gcagtgcatt gaggatattc tggcaataca
     6061 acccacgttg ccagcaagaa ttcatcgacc ttatttgcat aaaggggggc gagcctggtc
     6121 ccgggggcaa tatattcttg agcattatcg ctttgtgcaa aatttgccag aaaaatattc
     6181 tgaatttttg tttccacaaa aatcggtatc attagtccaa ttcatcggga aagatggaga
     6241 ggattttgat atccagtgtt ctcccagtgg ttttgaccgg gaaggagagt tgatgttgtc
     6301 attgtttttc aatcaaatag tgattgcccg gttaacattt tcagtcattc tgactcaaaa
     6361 tggacacacc gcttttattg gcggattaca gggagccccc aaaaataccg gacctgatgt
     6421 tattcggtgc gcaacccggg cctgttatgg gcttttccca aaacggatta tttttgaagc
     6481 attttgtgcg ttgatgaaag cttgtaatgt ctctgagtgt ctggcagtca gtgagcacag
     6541 tcatgttttc cggcaattga gatactggta tcagaaacgc aaaacctttg ttgctgtcta
     6601 cagtgatttc tgggagtctg tagcagggaa aacctgtggt gactggtaca aattaccaac
     6661 acaggtggtt cgaaagccgc taagcaatat agctagtaaa aaacgctctg agtatcgaaa
     6721 acgatacgct ttgctggatt atatccatga aactgctatc cgctctttgg atgcatatcc
     6781 tgtaaactca gaacatcagg acttaaatta aggtttgatg tattctctga ataaaatatt
     6841 aatgatgatt ttggtagggg cattcgcact aaataatgaa aaaatacaaa tccgagttta
     6901 ttcctgaatt taagaaaaat tatctttccc ctgtttactg gtttacatgg ttcgttttgg
     6961 gaatgattgc aggtatttca atgtttcccc cttcattcag agatcctgtc ttggccaaaa
     7021 tagggcgttg ggtgggcaga ttgagcagaa aagctcgtcg cagggcgacg attaatttat
     7081 cgctttgttt cccggaaaag agtgatacag aacgggaaat aattgtcgac aatatgtttg
     7141 ccacagcatt gcaatccata gtgatgatgg cagaactggc aattcgtggt ccggaaaagt
     7201 tccagaaacg agtgttctgg aaaggcctcg aaattcttga ggaaatccgg cacaataaca
     7261 gaaatgtgat ttttctggtt ccccatggct ggagcgtgga tattcctgca atgttgctgg
     7321 cagctcaggg ggaaaaaatg gccgccatgt ttcatcagca acgaaatcca gtgattgatt
     7381 atgtctggaa ttctgtacgg cgtaaattcg gggggcgctt acattcccgg gaggatggga
     7441 taaaaccatt tattcagtca gtacgccagg gatactgggg gtattacctt ccagatcagg
     7501 atcatggtcc tgaatacagt gaatttgctg atttttttgc gacctataaa gcgacattac
     7561 caattattgg acgtctgatg aacatcagtc aggctatgat tataccgctt ttcccggttt
     7621 atgatgaaaa aaaacatttc ctgactattg aagttcggcc accaatggat gcatgcattg
     7681 ccagcgcgga caataaaatg attgcccgac aaatgaacaa aacagtggaa attttggtgg
     7741 ggtcacatcc ggaacagtat atctgggttt taaaattgtt aaaaacgcgc aaatcaaacg
     7801 aagcggaccc gtacccttga aattataaag tacaggtatt tccactagtt gtttcttaca
     7861 ggttaccaat cgaaacacat cccccttccg tgtgttgccc gtttggcacg gaggactgtg
     7921 tagatgcagt tcgttaaatt taatttatgc ctaaatagct gcgcttaata ccgctacact
     7981 tttgccagcc catgtttgcc tccggggaat gggctgacgg tttccataaa atggcgaact
     8041 tttttcaaca gttgccacat tgagcggcac tgatgattac gcgttattgt gtcgtgaagt
     8101 gcctgccata gccgttcaac atgattcacc catggcgagt aaaccggctg ataaatgccc
     8161 ctgaacttcg ggttctcctt cagccagctc tgtgtttccc ggcttttgtg gataatgtag
     8221 ttgtccacga tcagcgtgat ggttttcgcc cgacggtatg tcgctttaag ccgcttcagc
     8281 aggctgatgt aacagcgccg aacttttgct gttgccgccc acatagctga ctttacctgt
     8341 cccgctgtgc agcgctccgg ccagataata tttttcattc tgtcccggcg tgaccacccg
     8401 tttttgctgt ccgcgcagtt gccagtccgc accgattttg ggatgaagat ggatatccac
     8461 ttcatcttca taaaagaccg gatgctctgc gctgcattcg tccagtgctt tatggattgc
     8521 tgccatcttt tcatctttat gcgggtcacg gatacgcaga gttggcgcag cccttcgcca
     8581 cacaatcccc gcagacggca accagcggcg aacggttccg gcatttaact ggcaaccggt
     8641 tatctcattg atttttattg ccagcagttc tgtactccag cgtgaacgct ggtagccaaa
     8701 gtcgccggga gaatgtttta ccagctcacg taacagtgtg cagatatgct caaacggcca
     8761 gcgacgggca cgcccggcag gtaatgattt cagtccctca acacccgact gcgtgaacca
     8821 gttaatccag cgtccaacag aggaacgggc gcagcagagc gttctggcaa cgtcgctgac
     8881 acggtcgccc cggtgcagca tcagcatggc agtcagtctg cgggcataat ttttatcgtg
     8941 tgttttatgg atggctttct gcatcaggcg tcgttcgtca cgggaaattg gtgctatgat
     9001 cggcattgct cagtccggtt ggtgatttgt tttgatttgg cgattgatca gatcgcacaa
     9061 tccgggctaa gttccctcaa agggatctac tattcc
//
LOCUS       DAJTNM010000140         9033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTNM010000140 DAJTNM010000000
VERSION     DAJTNM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.140"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="OO172_003530"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="OO172_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5513564.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="OO172_003531"
     CDS             962..1303
                     /locus_tag="OO172_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5513565.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="OO172_003532"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="OO172_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="OO172_003533"
     CDS             complement(1901..2611)
                     /locus_tag="OO172_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5513566.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="OO172_003534"
     CDS             2719..3024
                     /locus_tag="OO172_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5513567.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..3546
                     /locus_tag="OO172_003535"
                     /pseudo
     CDS             3223..3546
                     /locus_tag="OO172_003535"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4102..4701
                     /locus_tag="OO172_003536"
                     /pseudo
     CDS             4102..4701
                     /locus_tag="OO172_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(5293..6264)
                     /locus_tag="OO172_003537"
     CDS             complement(5293..6264)
                     /locus_tag="OO172_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513568.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTPIIAHTLSDRSVVVPVRGGERPEVVTVPSGQE
                     IVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMVDVLESS
                     KLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKALSRLAA
                     KQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITVVLSRDG
                     DSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA"
     gene            complement(6339..6848)
                     /locus_tag="OO172_003538"
     CDS             complement(6339..6848)
                     /locus_tag="OO172_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513569.1"
                     /translation="MSSKASALFNNNDWKLHVTDINLYENTVSLDGQSYPLSFAIKTL
                     IPGYLSGLPATSRESMELLEALAEAGVTIGNFFSNDLMTAYQRRQQNKRAEAERIAKE
                     QRIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADIE
                     SGGNWMDSL"
     gene            complement(6950..7171)
                     /locus_tag="OO172_003539"
     CDS             complement(6950..7171)
                     /locus_tag="OO172_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513570.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRRLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
     gene            complement(7418..8263)
                     /locus_tag="OO172_003540"
     CDS             complement(7418..8263)
                     /locus_tag="OO172_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513571.1"
                     /translation="MNTNEVSVINLDQLVSMSSYDFLTKVINPARVEAGEKPVRNNDF
                     VSRIEDELDGQNVAYEIFVSFGQDVKSYTLNTDQLLLVGMRESKAVRRKVLAYIRQKE
                     QEVLELQAQQLQVAQTKAICYAPGAIVLDSKLDSKSGATPTEVIKALDLKLTSSKMLT
                     ASVLTGYLKTLCRPNPFYTKRVTEFGTTDIDLSTGRPRAIFNKKGETNGYYRLQPQPN
                     DKEPTIKILRKGYDWLKDNWKDIAHDMRTSNIKVVRRLVDSHLNGKATKSQKAAAGKG
                     SDYGF"
     gene            complement(8489..>9033)
                     /locus_tag="OO172_003541"
     CDS             complement(8489..>9033)
                     /locus_tag="OO172_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HCR5513572.1"
                     /translation="YLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVG
                     WAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMS
                     RRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGL
                     PPNESENRYWKNSNSVASFC"
BASE COUNT         2238 a         2294 c         2257 g         2244 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg
     3301 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     3361 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     3421 cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat
     3481 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     3541 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     3601 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     3661 gtcgaacagt atggcaatga agctgtatat attcagtacc cttcggggat tgttggcggc
     3721 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     3781 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     3841 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     3901 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     3961 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4021 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4081 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4141 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4201 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4261 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     4321 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     4381 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     4441 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     4501 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     4561 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     4621 ttcatctgga actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt
     4681 aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc
     4741 attagacggg atgtctagac ggctggtcag gcccccggtg gcctctttgt caggactcag
     4801 ggtggcaccg gggaacattg gtgtcttaat tgtattgaat gaggtctcta acttttggtc
     4861 caaaactcat cgttagctgc tcgccagctc acagccgaac ccgaactgta agcacgacca
     4921 ccagcaccag cttcagcagg aagacgacaa gatagacttt atgcaggtcc atgcgtcctc
     4981 ctgtgtctat tgtaccactg cactagataa ctagtacaaa ataaagtcca gaccgaaggc
     5041 ccagaccata gttaattaat cacattgata atccaccacc tgaagtcctg ccaacattca
     5101 gtccagcctt acccatgcta ggagtcttaa ccggggtgtc gttctgtgtc tccaccactg
     5161 gtgccgcatc aggctctaca ggaagctcca cagcaggcgc agggaccgga ggtgatacgg
     5221 atgtaccacc accagcggag aagcccttag cgatggaccg gaggccttta ctaatgctct
     5281 tacccattgc tgttacgctt cggtcttggt gaacttcagg cgtttgacta caggcaggac
     5341 tgcggttgca gcgccagtgt ccagcttaac tgtcgtagca gcttcagcga caacgaagac
     5401 cttaccgagg atttccaagt cgtgggactg agagtcgcca tcacgtgaaa ggacgaccgt
     5461 aatattctca ccgtcgtgac ggaagcagcc gaagctcagg gccgtgaagc gtttagccag
     5521 catgaacaca cctttgtcct caccgttgta atacgcgatg tcggtaccca gcatatccac
     5581 gatagcgtct tcaccagaga agccctgttt agcagccaga cgggacagcg ctttcagttg
     5641 ggactcatgg gcgatgatgc cgtaatcaga cacttcagag ccgaaagagg actcggtgtt
     5701 ctcaaacaga gcgtccatga tagcgtctgc cttagtggtc aggcttccag tcaggactgg
     5761 cagctctacg gtcttcagca gcttggagga ctccagaacg tcaaccatgc gagcgttgat
     5821 ggtacggacc acattctcac agatgttcat agcgtcctgc tggagtgtgc catgagtgaa
     5881 accagtggag gacttggcgt cccacagttt agtttgctcc gagatgagga ttgcctgttc
     5941 gataggttcg acgacaatct cctgaccaga cggaacggtc acgacttcag gtcgctcacc
     6001 accgcgtaca gggacgacca cagagcggtc agacagggtg tgggcgatga ttggggtagg
     6061 caggacttct acagggaacg gaatgttatc ctgagcgtct ttaagtacac cgttcagcat
     6121 agccttaacc gtcttagcta actggagggc atagacctgt gggtcagcgt tagttggcag
     6181 accttcgtta acctgaatgt tgtcggtgtc ggtgatacgt actggctcaa tgccgaactg
     6241 atagctatct ttaatcatgg tcataataat caatctccta aattagtttt agatactgtg
     6301 ggtcccgaca tcaggtcagg accacttgtg tgtctgtctt agaggctgtc catccagtta
     6361 ccgcctgact caatgtcagc gacaatagcg gcacgggagc gaccagcgga atgggtagca
     6421 ctgcggaggt tctcaccata ggtccggcgc tcagctttga cctgttcgcg gcgctgtagc
     6481 tctttctgcc actctgcatc ggtcatgttc tcttcacgca tacgctcagc ctgaatgcgc
     6541 tgctctttag cgatacgctc agcctcggct cgcttgttct gctggcgacg ttggtaggcg
     6601 gtcatcaggt cattgctgaa gaagtttcca atggtcacgc cagcttcagc cagagcttcc
     6661 agtaattcca tggactcgcg gctggtggca ggcagtcctg acaggtagcc cggaatcagg
     6721 gtcttaatag cgaaggacag tgggtaggac tgaccgtcca gactcacagt gttttcgtag
     6781 agattgatgt cggtgacatg gagtttccag tcattgttgt tgaataacgc actagctttg
     6841 ctagacatag taattactcc tcttgagagt tgagactatc ggtaccgcag acacagttat
     6901 gcctacgggt ctccccgcat cggtcatctc aaaaggttta aattaggggt tagtgagtgg
     6961 cgtccagctt agcgaacgct cggcgtaact gattgttcag gtcacggtcg accagcaggt
     7021 ctatcagttc gtctcgacta tagaaaggac gctcagggtc ctctacgccc atccccagca
     7081 ttgacaggag gcgacgggta ggcagaccac ggtaacggag agtagccttt ccgatgggtg
     7141 tccgtgggtc gatatgttgt ttagttgtca ttgttagaag tcctcgttag accaatcatc
     7201 cacgtcaaat tcactggtga taggagcttg gaagttaggt agaccctctg gtatctctag
     7261 aggcagtcgc tcgtcgactc tcagtgccat ctcttcgtgt atatgccgaa gcagagaagt
     7321 cagtaagata gggtccgcag gatgctcagg gccgtgtctc tcaatgaaac atatggttgt
     7381 cgctattgac tccataatca gttcatcagt gagtcgctta gaacccatag tcagacccct
     7441 tgcccgctgc tgctttctgg gactttgtag ccttaccatt caggtgagaa tccaccagac
     7501 gacggacaac cttgatattg gatgtgcgca tgtcatgagc gatatccttc cagttatcct
     7561 tcagccagtc ataacccttg cgtagaatct tgatggtagg ctctttgtcg ttaggctgtg
     7621 gctgtagacg atagtagccg ttggtctcac ctttcttatt gaagatggct cgtggacgtc
     7681 cggtcgatag gtctatatcc gtagtgccaa actcagtaac acgcttggtg tagaatgggt
     7741 taggacgaca cagggtcttc agatatccag tcaggacgct agctgtcagc atcttagagc
     7801 tggtcaactt aaggtccaga gctttaataa cctcggtagg cgtggcgcct gacttggagt
     7861 ccagcttgga gtcgaggacg atagcaccgg gcgcatagca gatagctttg gtctgtgcga
     7921 cctgaagttg ctgggcctga agttcgagga cctcttgctc cttctggcga atataggcta
     7981 ggaccttacg acgcacagct ttagactcac gcatcccgac cagcagaagc tggtcagtgt
     8041 tgagggtata gcttttaacg tcctgcccga agcttacgaa aatttcgtag gctacatttt
     8101 gaccgtccag ctcatcttca atacgggaca caaagtcgtt gttacggaca ggcttctccc
     8161 cagcctccac acgagccggg ttgatgacct tagtcaagaa atcgtagcta ctcatagaca
     8221 caagctggtc aaggttgatg acgctaactt cgttggtatt cataaaggat gctcctttgg
     8281 taaagcactt tctgtctcca tgtggagtcc gcacctattc aatgcatcca aacggaccca
     8341 ccaaaaagtg cttaacgaaa gtgaataggg ttaatgttga ttagtgttgg tctggagtga
     8401 ccatgttctc catgtgcata taaggcgaga ccgcatgtca ggacgagctg taaggacccc
     8461 agatggtgag cggtgataga tgaagtggtc aacaaaaact ggccaccgag ttagagtttt
     8521 tccagtatcg attttccgat tcgtttgggg gtaaccctcc gttatattcg tgcggtctta
     8581 gtgcgctgta atatccaacg atatagtccg ttatggcgtg agctgcctcg ctgaagctta
     8641 cgtaacccac caccggcatc cattcgttct tcagactcct gaagaagcgt tccattgggc
     8701 tgttatccca gcagtttccg cgccggctca tactctgtct gatctggtat cgccacaata
     8761 actgccggaa ctgcctgctc gtataatgac tgccctgatc gctgtggaac atcaccccgc
     8821 cgggcttacc acgggtttcc catgccattt ccagcgcttt catggtgagc ctgctgtccg
     8881 gcgagaacga catggcccag cccactggtt ttcttgcgaa caggtcgaga acaacggcga
     8941 ggtacgccca gcgcttaccc gtccagatat aggtcacatc accgcaccac acctgatttg
     9001 gctcggtcac ggcgaactgc ctttcaaggt agt
//
LOCUS       DAJTNM010000141         9029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTNM010000141 DAJTNM010000000
VERSION     DAJTNM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.141"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO172_003542"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO172_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5513573.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="OO172_003543"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="OO172_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO172_003544"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO172_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5513574.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="OO172_003545"
     CDS             complement(2403..2606)
                     /locus_tag="OO172_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513575.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="OO172_003546"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="OO172_003546"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5513576.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="OO172_003547"
     CDS             complement(3802..4014)
                     /locus_tag="OO172_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5513577.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="OO172_003548"
     CDS             4274..4582
                     /locus_tag="OO172_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5513578.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO172_003549"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO172_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5513579.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO172_003550"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO172_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5513580.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="OO172_003551"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="OO172_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5513581.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="OO172_003552"
     CDS             complement(8478..8981)
                     /locus_tag="OO172_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5513582.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2235 a         2314 c         2148 g         2332 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcagcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagaacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc agtggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctac tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattat
//
LOCUS       DAJTNM010000142         8935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTNM010000142 DAJTNM010000000
VERSION     DAJTNM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.142"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..2886
                     /locus_tag="OO172_003553"
                     /pseudo
     CDS             1..2886
                     /locus_tag="OO172_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2996..3844)
                     /gene="alkA"
                     /locus_tag="OO172_003554"
     CDS             complement(2996..3844)
                     /gene="alkA"
                     /locus_tag="OO172_003554"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR5513583.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3978..5330
                     /gene="yegD"
                     /locus_tag="OO172_003555"
     CDS             3978..5330
                     /gene="yegD"
                     /locus_tag="OO172_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5513584.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5342..7282)
                     /gene="yegI"
                     /locus_tag="OO172_003556"
                     /pseudo
     CDS             complement(5342..7282)
                     /gene="yegI"
                     /locus_tag="OO172_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7279..8040)
                     /locus_tag="OO172_003557"
     CDS             complement(7279..8040)
                     /locus_tag="OO172_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR5513585.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8037..8696)
                     /locus_tag="OO172_003558"
     CDS             complement(8037..8696)
                     /locus_tag="OO172_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5513586.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2175 a         2369 c         2330 g         2061 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgtgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtggtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct ctgtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc caattttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggttacgaa
     2101 ccacagcaag ctgccgcaca tagcgaacgg gcggcgatgt cgcttgatga acagtggcgg
     2161 atgattaaag agaatcagtt gatgatgatc gcccacggtg tcgcttcgcc gcggatcccg
     2221 gaagcgcgta atttgtggct gatttcactt aagctctgga gttgcgaagg cgagattatt
     2281 gatgaacaaa aatttcgtcg tagcttcagc gatccggcac ttagccatgc tcttgaccga
     2341 cgggtattcc acgaattttt ccagcaggcc gcaaaagcgg ttgccagtaa aggcataagc
     2401 atcgccctcc ccctttccgt tgccggtttg agtagcgcca cgctggtgaa tgatctgctt
     2461 gagcagctgg aaaatagccc tctaccacca cggttattac atctgattat tccggctgaa
     2521 gcgattttag atcacgcaga aagcgtgcaa aaactgcggc tggcgggatg tcggatagtg
     2581 ctcagccagg tgggccgcga tctgcaaatc ttcaactcgc tgaaagcgaa tatggcagat
     2641 tacctgctac ttgatggtga gttatgcgcc aacgtgcagg gtaatttgat ggatgagatg
     2701 ctgattacga ttattcaggg gcacgctcag cgactcggga tgaaaaccat cgccgggcca
     2761 gtcgttttac ccttagtgat ggatacgctt tctggcatcg gcgtcgatct gatttatggt
     2821 gatgtgattg ccgatgccca accgctggat ttgctggtga atagcagtta tttcgcgatt
     2881 aactgagaga gcgtttgccc attgcctgat gcgatgctaa cgcgtcttat caggcctaca
     2941 aatcgctcat tccgcaggcg ggataaggcg ctcgcaccgc ttccggcgac caacgtcatg
     3001 cttcgtctgg ttgccagcct tccgtatacc agatatgcaa cagcgcataa gaacgccagg
     3061 gcttccagcg ctcggcataa cggcggattt gtgccggtgt catccccaga aatcgctgtt
     3121 taatcagata atcatccggc agaaaaacat ctttcgcctg ccagccgcgc aaggcgaaat
     3181 aattcgccgt ccagcgcccg atacccggaa aagtttgcag cgttttcatc gcctgctcca
     3241 catcgcccgg tattgtcatt ggtaaggtgc cctccagcgc cgcatttgcc agatgaatca
     3301 gcgcctctgc ccgtttcaac ggcataccta acgcttttaa tgcctgcggg tcggctgctg
     3361 ccagccgctg aggcgtgggg aagcagatat actccggaaa atcatccagc cgttcgccat
     3421 aaagctgtgc cactctggcg gtcaattttg ccgccatcgc cacgctcacc agttggccta
     3481 aaatcgcccg cacgccctgc tcaaaagcat caacactgcc gggtaaacgc aatccgggcc
     3541 gcgccgcgcc taacttgccc aacgcaccgt taacaatctg cgggttacat tgcagatcaa
     3601 gcaggcggct cattttcgcc agacactcag cggcaacggg ttctaaacct gcacttaaat
     3661 ttatgtgcag agtatggcgg gctatatccg gaatagcagt caccacgccg cgatattcgc
     3721 ccactgccag actacgggca taataactgt ccgcgaccgt ttccacacca ctcaccgcac
     3781 gggcggcgag aaatcccaac atccacgacc agtcatacgg cggctgccag ttcaggatat
     3841 acatcgcatc tccttattca tccgctttca gcataaacgt tattcagacg ttacgctttg
     3901 ctttcatatt ccggttgtcg cgacggcaac atttcgctaa agtcacgccc cttcttcacc
     3961 ggcatgggga ttatttcgtg tttattggtt ttgattacgg tacagcaaac tgttcagtgg
     4021 cggtcatgcg tgacggtaaa ccgcatttgc taaaaatgga aaacgacagc acgctgctgc
     4081 cttcaatgct ttgcgcgcca acgcgtgaag cggtaagcga atggctgtac cgccatcatg
     4141 acgttccggc agacgacgat gaaacgcagg cgttgttacg acgggcgatt cgttataacc
     4201 gcgaagaaga tatcgatgtt acagcgaaaa gcgtgcagtt cggtctttcc tcactggcac
     4261 agtacattga tgatccagaa gaagtgtggt ttgtgaaatc accaaaatcg ttcctcggtg
     4321 ccagcggctt aaaaccgcag caggtagcgc tgtttgagga tctggtctgc gcaatgatgt
     4381 tgcacattcg ccagcaggcg caggcgcagc tgccagaagc aattactcag gcggtgattg
     4441 gtcgcccgat caacttccag gggctgggcg gcgatgaagc aaacgcccag gcgcagggga
     4501 ttctggaacg cgcggcgaag cgtgccgggt ttaaggatgt ggtattccag tacgagccgg
     4561 tagcggctgg gctggattac gaagccacct tgcaggaaga aaaacgggtg ctggtggtgg
     4621 atatcggcgg tggtacgact gactgttcat tgctgctgat ggggccgcag tggcgttcgc
     4681 gtctcgatcg tgaagccagt ctgctgggtc acagtggttg tcgtattggc ggtaacgatc
     4741 tggatatcgc cctagcgttt aaaaacctga tgccactgct gggtatgggt ggcgaaaccg
     4801 aaaaaggcat cgccctgccg atcctgccgt ggtggaatgc ggttgccatc aacgacgttc
     4861 ctgcacagag tgatttctac agtagtgcca acggtcgtct gcttaacgat ctggtacgcg
     4921 atgcccgcga accggaaaaa gtggccctgt tacagaaagt ctggcgtcag cgtttaagct
     4981 atcgcctggt gcgtagcgca gaagagtgca aaattgctct ttcaagcgta gcggaaaccc
     5041 gcgcctcact gccgtttatc agcaatgaac tggctacgct gattagccag cgagggctgg
     5101 aaagcgccct cagccagcca ctggcgcgga ttctggaaca ggtgcaactg gccctggata
     5161 acgcccagga aaaaccggac gttatctacc tgaccggcgg tagcgcccgt tctccgctga
     5221 ttaaaaaagc gctggcagaa cagttgccgg gcattccgat tgcaggcggc gatgactttg
     5281 gctccgtcac cgccgggctg gcacgctggg cggaagtggt atttcgttaa aagaaaatgg
     5341 ctcagcagcg gcttaaatca gcctgcgcct gagccagttt ttctgccgcc tgacgtaacg
     5401 gttccatcaa cggcatggtc cgtgctggag catgaaggcg gaatcgctgc aactctgtcg
     5461 cgccgacagt caacgtcgat tccagccgat gtcgtttggc ggccattttc gccattaccg
     5521 cctgtctgtc tgctggcgtt accgcttcgt tcggcctgaa cacaaaacgg cgttcacagc
     5581 tcgctttcca gtcgatgacc gcctgggtca gatgatcacc aaacccttta acttgcttaa
     5641 cgccacgacg ggtaacacct gctgctgttt caataccaaa agaccataac gccgctttac
     5701 gcgcagggcc aacgccggga atagaggcaa catcaataaa aaatccctcc agaaacttct
     5761 acttctgccg ttcccttgca gtgtcatgaa gtgctgccag agcgagtttc tcttcttccg
     5821 gtaacccgag aatttcgtcc ttcatttttt cgagcatcgt ccgtttggcg ataaaacctt
     5881 ccaggccgcc tacctgctgg atctggcgga ctaaatggtc atagtccatt ttcgcacgat
     5941 taaatgcctc acggcgttgc tgaacttctg ctttgtacgc tttgcttgtc agactgccaa
     6001 taatccagat agccgccagc acaggaacca aaataatata acgcggttct gcctgaaggc
     6061 cgcaaagcag cagcgataat gctgacagtg cgatctcaag cagaatgatg tattcgcgcc
     6121 gtaacaggcc taaaggaagc ggcctgccag tcggttggaa atgatcgggt aatggcagtt
     6181 gcaatgctgg cggtgctact gacgccatca ccatcgccca gacttttgcc agcacaaaat
     6241 ccccgccggt ggtaatgacc tcttcgccga gatcaataaa atagataacg ccttgattat
     6301 ccagcgtaca ccacaggcag tcggtcaaat gagcgggata aacatgcatt gccgaaacgg
     6361 gacatttctt taactgttgg cgcagagaat caagcgccgc tacccacgct ttagccgtcg
     6421 gacgcccggt tgccacgcca ctttccgtga atgcctgctg aaacatggct tcaacatcac
     6481 tcggtaacat cgatagcgga atcgatcgtg gcggcggttt taaaccacgt cgctgattat
     6541 ctgacgcgta ggcataacgg aaatgggtaa tatccgtctc cagcgcatta cccgcatcag
     6601 agataagcgg cacaccagaa taaggatgcc ggccaccaaa caagacgtga aaaatcagca
     6661 acgcaaggcc aaaattaccg tgattttctg tgcgttcaaa gccgacaaat gacgacaagg
     6721 tttgtagctc tggcggcgta aaatgcgaca cgccgacttc gcataaatgc agtgtgccat
     6781 tggcgttaat ctgaaaggag tcgctatcaa tcaacaccac tttgctgtcg cgacctacca
     6841 taaaactgtt ctggtttacg tcacccacga catgcccgtg ctcgtgaacc gtagcaaaag
     6901 atgaagcaat attgcgcgca acatagagta gaaaatccca cgcacaatga gggtaacgtt
     6961 gacgacgatg tgccgggcta tagatcatat gaatcggttc tttaccagaa acttttggca
     7021 tcataaaacc gataactttt ccgcctcgtc caccgtgaag cgttgcctgc ggccaggcga
     7081 cataattcaa caattgcgcg tcagctgtcg cagccataaa ggcaagtttg tcctgtttta
     7141 aggcgggtgg cggcgtgtga taaatcttgg cgacgctatc gacaaactca ttgatatcat
     7201 aaaccgcgcc ttcaccgcct ttgcccagtt cacggcctgg cgtaacgcac tcgccagtag
     7261 cagtatataa agtgggtttc atggtaacca cagcgccagt gccaacgttt tatcatcatc
     7321 cgtacgctcg tttaccgccg ggctggacaa aaactgcttt aacaattcag gtaataaatc
     7381 gagttgttcc tgcgttgctg acgccagtcc attgaaaaac ggggtaaaga aaggcacatg
     7441 cggagaatta tccaacatat ttaacgccag tcgctggata ccatccgtaa atgctgcaac
     7501 tttatgcaca cgctcagtgc tggtgaaagt ttccagtctg gaaacggcat cttcatcggt
     7561 aatgaagtgc gtcatattgg catattcacc caccatcggc gtaagcggca attgcagacc
     7621 gtgaccaaaa tccaccacca cgccgccatc gccaatctgc ataatcaagg tgccattagc
     7681 cgatgaaatc aatcccagaa acgtgcaggc aaaatcgcga actgctaatt ctttggcttc
     7741 cgcctcggca aaaagtcgct gacgaacggt cagcacaata tccgtggcga ggatatcatt
     7801 aagccccaat tccccaccct gcactttttg cgacatataa gccatcgctt cattgacggc
     7861 gagcatcgcg ccttcgcccc cctgcgaaac gctacctgcg ccatcagcaa ggaacatcac
     7921 cagtaatggc tgctggtcat ttagccaggc aacctgcatc tggcaggcat cctggcaagg
     7981 caagtccgcg ctgatgtgcg acgttccaac agcggatgca taaaccagac gccagctcac
     8041 actgacgtcc agccttttgg cgcttccagc acaacttccg ttcccggcgt ggagcgggaa
     8101 accgaacgca gcgaactgga taaccagcta aacagttcac ggaattgtaa tccttgcaga
     8161 ggcaaaggct gacgaacgct gatttgtgcc agagtcttca tatccgcccc ctgaacaccg
     8221 atggaaaaga aagcaaacct tttatcttct tccccccgaa acactttgtt ggctgcagcc
     8281 tgccactcat cggttggtgc accatcggta atcaggaaaa tccacgggcg ataatacgaa
     8341 ataccatttg cacgatactc acgtttccgc tcctctacca tatccagggc tttggtaatg
     8401 gccgcaccca ttggtgtatc gccctgggca aacaggatgg gcggaaaaaa attagcggcg
     8461 ctggtaaagg gttgttccac atgcaccggg ccgaacgtca caatgccgag ttccactctt
     8521 tttaaagcca gcgaatcggc aagcagctca tcgcgaaagg taaccaatcc ggtgttaagt
     8581 tcattaatag gtctgccact catcgagcca gaaacatcca gcagcagaat gcaggggcaa
     8641 cgtggttccg ggttactggc aaaatcgctg gtggcaaacg tgatttgttc gctcataaga
     8701 aatcctttct tagagaatta ttgaggtccg gtatcatacc gactgcaata atgaaataag
     8761 agcaaacgaa gaaactttca gaattagtcc cggcggcgca gaaaattttg ctattcgcct
     8821 cgcaagaacg gagagccgtc gattgacact aagggcggag tgacataatt tcaggagtga
     8881 gggttaggga gaggtttccc ccgttacttt attcgtaccc cttataatgg ggtgt
//
LOCUS       DAJTNM010000143         8900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTNM010000143 DAJTNM010000000
VERSION     DAJTNM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.143"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..1275)
                     /gene="trkA"
                     /locus_tag="OO172_003559"
     CDS             complement(<1..1275)
                     /gene="trkA"
                     /locus_tag="OO172_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5513587.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDN"
     gene            complement(1297..2586)
                     /gene="rsmB"
                     /locus_tag="OO172_003560"
     CDS             complement(1297..2586)
                     /gene="rsmB"
                     /locus_tag="OO172_003560"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5513588.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2632..3579)
                     /gene="fmt"
                     /locus_tag="OO172_003561"
     CDS             complement(2632..3579)
                     /gene="fmt"
                     /locus_tag="OO172_003561"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5513589.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3594..4103)
                     /gene="def"
                     /locus_tag="OO172_003562"
     CDS             complement(3594..4103)
                     /gene="def"
                     /locus_tag="OO172_003562"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5513590.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4251..5375
                     /gene="dprA"
                     /locus_tag="OO172_003563"
     CDS             4251..5375
                     /gene="dprA"
                     /locus_tag="OO172_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5513591.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5347..5820
                     /locus_tag="OO172_003564"
     CDS             5347..5820
                     /locus_tag="OO172_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5513592.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5849..6391
                     /locus_tag="OO172_003565"
     CDS             5849..6391
                     /locus_tag="OO172_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5513593.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6396..6968
                     /gene="tsaC"
                     /locus_tag="OO172_003566"
     CDS             6396..6968
                     /gene="tsaC"
                     /locus_tag="OO172_003566"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5513594.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            6973..7791
                     /gene="aroE"
                     /locus_tag="OO172_003567"
     CDS             6973..7791
                     /gene="aroE"
                     /locus_tag="OO172_003567"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5513595.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7788..8045
                     /locus_tag="OO172_003568"
     CDS             7788..8045
                     /locus_tag="OO172_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5513596.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8021..8575)
                     /locus_tag="OO172_003569"
     CDS             complement(8021..8575)
                     /locus_tag="OO172_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5513597.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2219 a         2176 c         2229 g         2276 t
ORIGIN      
        1 attgtcattg gcaatcatca cgtcgtttcc acgtaccacc gctccaataa tcgttcctgg
       61 cggtagcttg atttcgtcaa tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg
      121 agcaacggct tcaatagctt ctgctacgcc gcggcgcaat gaggaaacac caacaatatc
      181 tgcttttcgc acatggctaa gcaacgcaga aatagttgct tgttgtggtg aaattgcaat
      241 atcgatcacg ctcccctgaa ccagatccac ataagcgcga cgctggatca ataccatcac
      301 ctttttcgca cccatacgtt tggcaagcat ggcggacatg atattggcct cgtcatcgtt
      361 ggtgacggca ataaacagat caacttgatc gatatgttct tcggccagca gttcttgatc
      421 cgacgcatca ccaaaaaaga cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg
      481 ctgctgatta cgttcgatga gtttaacgct gtaatctttt tccagacgac gcgccagccc
      541 tgcaccgata ttaccgccac caaccagcat gatccgttta tacggttttt ccagtcgctg
      601 taattcactc atcaccgcgc ggatatgctg tgaagcggca ataaagaaca cttcatcacc
      661 agcttcaaca atggtcgaac cttgtggacg aatggggcga tcgtggcgga aaattgctgc
      721 cacacgagta tcgatatgtg gcatatgttc gcgcatggtc gaaagtgcat taccaatcag
      781 cgggccgcca taataggctt taactacagc gaggctgact ttaccctcag cgaagttcac
      841 cacctgcaat gcgcctggat actcaatcag tcggtaaata ttatcgataa ccaactgctc
      901 tggtgcgatc agatgatcaa tcggtacagc atctgaatga aatagcttat cggcatcgcg
      961 tacgtagtct ggtgagcgga tgcgagcgat gcgattaggg gtgttgaaaa gtgagtaggc
     1021 tacctggcag gcaaccatat tggtttcatc tgaactggtt acagcaacca gcatatcggc
     1081 gtcgtcggca cctgcctccc gcaatacgcg tggatgagag ccatgcccct gcacgactcg
     1141 caggtcaaat ttatcctgca aggtccgcag acgctcaccg ttggtatcga caacagtaat
     1201 atcgttgttc tcgccaacca ggttttccgc cagtgtgccg ccaacctggc cggcacccag
     1261 aatgataatt ttcatcagtc gcgacccgtt atctcatcac tttttgatta gcttagcgta
     1321 aaagaagccg tcgccctctt cggcaccagg ctgattttgt ttacccggtt gctctggtgt
     1381 tcctgtttcg caaagttcgg catcagcggt acgttgcaaa aaggctttaa tctgcaggct
     1441 attctcttcc ggtaacaccg aacaggtggc atagaccagg gttccaccgg attttaaatg
     1501 cggccaaatg gcgtcgagaa tttcagactg caattgcgcg agttccggga tatcgcgatc
     1561 gcgacgtaac catttaatat ctggatggcg acgaatcacc cccgttgctg aacaaggcgc
     1621 atctaataaa atgcgatcaa actgttgctc gccacaccat tgggaagggt aacggccatc
     1681 accttgtttc acggttgcct tcataccaag gcgttttaaa ttgtcgtaaa cgcgagagag
     1741 gcgctgttcg tcaatatcaa ccgcaacaac ctgcgcttct ggtgccacct caaggatatg
     1801 cgttgtttta ccgccggggg cggcacaaag atccaagatg tgttcaccgt tttgtggcgc
     1861 aagccaggtc atgcaacctt gtgctgatgc atcctgaacg gtaacccatc cgtcttcaaa
     1921 accaggtagc gcatgaacag gtgcaggtgt ttccagccgt acagcatcag ggtaatccgc
     1981 atgcgggaaa cctttcattc ctgcttcatc cagcaatgca agccagctgt cgcgggaatg
     2041 atgcgtacga ttaatacgca gccacattgg cggacgctgg ttattggctt cggcgatgga
     2101 ttgccactgc tctggatacg ctttttgcag acgcttcagc aaccaggaag gatgcagata
     2161 acgtgcatca cgggtattaa actcggctaa taactcttct tgccgacgct ggaactggcg
     2221 taatacaccg ttaatcaacc ctttaagttg cggacgctta attgcgatag cgccttcaac
     2281 cgtttcagcc agcgcagcat gaggtggaat gcgggtataa agcagttgat acaaaccaac
     2341 cataatcagg taatgcacag tgcgctgttt gccggtcatc ggacgggcca ttaacttatt
     2401 aatcagccag tctaactgcg aaagcgtacg cagtacgcca aagcacaact cttgaagaag
     2461 tgctttgtct ttatcggaaa ctttttgctg gagcggtggc agaatgttgc ttaatgattg
     2521 cccttgctcg acgacttgtt caacggcctg ggccgccatg ctacgtaaat tacgttgttt
     2581 tttcataagt ataaaaatgc tcggcaagac cgggcttaga agagtggact atcagaccag
     2641 acggttgccc ggaacaaacc attcccgacg agagttcagg aggtcttgcg cgctcatcgc
     2701 tttcttaccc gcaggttgta acgagagcag gttcaggatg ccatcaccag tcgcaacctg
     2761 aatgccttgt ttgttggctt caaggatcgt tcctggtgca gcgttggttg ccgtatcaat
     2821 gaccgacgct ttccagactt taaccggctg tccttcaatt tccagccagc tcattggcca
     2881 tgggttgaaa gcgcgaatgc agcgttcaag ctgtgctgcc gaaagtgacc agtcaatacg
     2941 cgcttcttct ttactcaact tctcggcgta agtgacaaga gtttcgtcct gaacttctgg
     3001 tttcgccgtg ccgtctgcca attgtttcaa cgtggtgata agcccttgtg ggccaagctc
     3061 tgccagcttg tcgtacagcg taccactggt atcttctgca gtaatcgggc aggagagctt
     3121 atagagcatg tcaccggtgt ctaaaccaac atccatttgc ataatggtta caccagtttc
     3181 tgcatcaccc gcccatagtg agcgttggat tggtgcagca ccgcgccagc gtggcagcag
     3241 tgaaccatga acgttgatac agccaagacg cggcatctcc agcacagctt taggcagaat
     3301 taaaccatag gcgacgacga ccataacatc agcctgcaaa tcggcgacca gttgctggtt
     3361 ttcttgtgga cgcagggaaa caggttgaaa aacgggcaga ccttcttcct cagccagaac
     3421 tttaatcggg ctgggcatca gttttttacc gcgtcctgcc ggtcggtctg gctgggtgaa
     3481 cacgccaacg acgttatgac cagaagacaa cagcgcgtcg agatgacgcg ctgcaaagtc
     3541 aggtgtaccc gcaaaaataa tacgtagtga ttctgacacg ttagttctta tccttaagcc
     3601 cgggctttca gacgatccag tttttcaact ttctgacgaa ttcgttgttg tttcagcggt
     3661 gacagataat ccataaacag tttgccaacc aggtgatcca tctcatgctg aatacagata
     3721 gctaacagac cctctgcttc cagttcaaat ggtttaccgt cgcggtcaag ggcgcgaatt
     3781 ttaactttct ctgcgcgcgg cactaaagca cgttgttcag ggatcgacag gcaaccttct
     3841 tcaatgcctg tttcgccgct tttttctaaa agctccggat tgattaacac cagccgttcg
     3901 tcacggtttt ccgaaacatc aataacaatg atacgttgat ggatatcaac ctgggttgcc
     3961 gccaggccaa taccttcttc tgcgtacatc gtctcgaaca tatcatcgac gatacgctga
     4021 atttctgcat tcacttcttc taccggttta gcaactttgc gaagccgctc gtccggaata
     4081 tgtaacactt gcaaaactga cataaatctc cagagatgtg ttcaggagtt agaaagatta
     4141 tttcttctat tctagacaaa tccccctctg attgacagca tcacttgatt gacagcatca
     4201 ctgaccaatc gcaaagattg ctaaggcttg cttatgacag ggagataagg atggtcgata
     4261 cagaaatttg gctgcgttta atgagcatca gcagcttgta cggcgatgat atggtccgta
     4321 tcgctcactg gctggcaaaa cagtcgcata ttgatgcggt tgtattgcag caaacagggc
     4381 ttacattgcg gcaggcacaa cgctttcttt catttccacg aaagagtatc gaaagctcac
     4441 tttgttggtt ggagcaaccc aaccatcatt taatccctgc ggacagcgaa ttttatcctc
     4501 ctcaacttca ggcgacgaca gattaccccg gcgcactgtt tgttgaagga gaactgcacg
     4561 cgctgcattc atttcagctt gccgtagtgg ggagtcgggc gcattcatgg tatggcgagc
     4621 gatgggggcg attattttgc gaaactctgg cgacgtgtgg agtgacaatt acgagtggac
     4681 tggcgcgtgg aatcgatggt gtggtgcata aagcggcctt acaggtaaat ggcgtcagca
     4741 ttgctgtatt ggggaatgga cttaatatca ttcatccgcg tcgccatgcc cgactggctg
     4801 ccagtctgtt tgaacagggt ggcgctctcg tctcggaatt tcccctcgat gttccacccc
     4861 ttgcttacaa tttcccacga agaaatcgca ttatcagtgg tctaagtaaa ggtgtactgg
     4921 tggtggaagc ggctttgcgc agtggttcgc tggtgacagc acgttgtgcg cttgagcagg
     4981 ggcgtgaagt ttttgccttg ccaggtccaa tagggaatcc gggaagcgaa gggcctcact
     5041 ggttaataaa acaaggtgcg attcttgtga cggaaccgga agaaattctg gaaaacttgc
     5101 aatttggatt gcactggttg ccagacgccc ctgaaaattc attttattca ccagatcagc
     5161 aagacgtggc attgccattt cctgagctcc tggctaacgt aggagatgag gtaacacctg
     5221 ttgacgtcgt cgctgaacgt gccggccaac ctgtgccaga ggtagttact caactactcg
     5281 aactggagtt agcaggatgg atcgcagctg tacccggcgg ctatgtccga ttgaggaggg
     5341 catgccatgt tcgacgtact aatgtatttg tttgaaacct atattcacac tgaagctgag
     5401 ttgcgtgtgg atcaagacaa acttgaacag gatcttaccg acgcaggttt tgagcgagaa
     5461 gatatctaca atgccctgct atggctggaa aaacttgctg attatcagga agggttggca
     5521 gaaccgatgc aactggcctc ggatcctctc tccatgcgta tttatacacc ggaagagtgt
     5581 gaacgactgg atgccagctg ccgtgggttt ctgcttttcc ttgagcagat tcaggtgctc
     5641 aaccttgaaa ctcgtgaaat ggtgatagag cgagtgctcg cgctggataa cgcagagttc
     5701 gaactggatg atctgaaatg ggtgatcctg atggtgttgt tcaacatccc gggctgcgaa
     5761 aatgcgtacc agcaaatgga agaattactc tttgaagtga atgaaggtat gctgcattaa
     5821 tcatttcttt aattcagcat aagttgttat ggcaaaatca gcactgttca cggtgcgtaa
     5881 taatgagtcc tgcccaaagt gcggggctga actggttatt cgatccggga aacacggtcc
     5941 gtttcttgga tgctcacagt atccggcgtg tgactacgtc cgtcctctga aatcttcagc
     6001 ggatggacat attgtcaaag ttctggaggg gcaggtttgc cccgtatgtg gcgcaaatct
     6061 ggtattacgc cagggacgct ttggtatgtt tattggttgc agtaactacc ctgaatgcga
     6121 acataccgaa cttatcgata aaccggacga aacagcaatt acatgccccc aatgtcggac
     6181 gggccatctg gtccagcgcc gctcccgtta tggcaaaaca tttcactcct gtgatcgcta
     6241 cccggagtgt caatttgcca ttaacttcaa accaatagct ggagaatgcc ctgagtgtca
     6301 ttatccgcta ctcatcgaaa agaaaaccgc gcagggtgta aaacactttt gtgccagtaa
     6361 acaatgtgga aagccggttt cggcggaata ataacgtgaa taataacctg caaggagacg
     6421 ctatcgcagc tgcgatagat gtcctcaatg aaaaacgtgt catcgcctat ccaacggaag
     6481 ccgttttcgg tgtcgggtgc gatcctgata gcgaaacagc agtgatgaga ctgttggagt
     6541 taaaacagcg tccggttgat aaggggctga ttttaatcgc ggcaaattac gagcagctta
     6601 aaccctatat tgatgacacc atgttgactg acgtgcagcg tgaaaccatt ttttcctgct
     6661 ggccaggtcc tgtcaccttt gtctttcccg cgcctgcgac aacaccgcgc tggttgacgg
     6721 gccgctttga ttcgcttgct gtacgagtca ccgaccatcc gttggtggtt gctttgtgcc
     6781 aggcttatgg taaaccgctg gtttctacca gtgccaactt gagtggattg ccaccttgtc
     6841 gaacagtaga cgaagttcgc gcacaatttg gcgcggcgtt cccggttgtg cctggtgaaa
     6901 cgggggggcg tttaaatcct tcagaaatcc gcgatgccct gacgggtgaa ctgtttcgac
     6961 aggggtaaca taatggaaac ctatgctgtt tttggtaatc cgatagccca cagcaaatcg
     7021 ccattcattc atcagcaatt tgctcagcaa ctgaatattg aacatcccta tgggcgcgtg
     7081 ttggcaccca tcaatgattt catcaacaca ctgaacgctt tctttcgtgc tggtggtaaa
     7141 ggtgcgaatg tgacggtgcc ttttaaagaa gaggcttttg ccagagcgga tgagcttact
     7201 gaacgggcag cgttggctgg tgcagttaat accctcaagc ggttagaaga tggacgcctg
     7261 ctgggtgaca ataccgatgg tgtaggcttg ttaagcgatc tggaacgtct gtcttttatc
     7321 cgccctggtt tacgtattct gcttatcggc gctggtggag catctcgcgg cgtactactg
     7381 ccacttcttt ccctggactg tgcggtgaca atcactaatc ggacggtatc ccgcgcggaa
     7441 gagttggcta aattgtttgc gcacactggc agtattcagg cgttgggtat ggacgaactg
     7501 gaaggtcatg agtttgatct cattattaat gcaacatcca gtggcatcag tggtgatatt
     7561 ccggcgatcc cgtcatcgct cattcatcca ggcatttatt gctatgacat gttctatcag
     7621 aaaggaaaaa ctccttttct ggcatggtgt gagcagcgag gctcaaagcg taatgctgat
     7681 ggtttaggaa tgctggtggc acaggcggct catgcctttc ttctctggca cggtgttctg
     7741 cctgacgtag aaccagttat aaagcaattg caggaggaat tgtccgcgtg aatcaggcca
     7801 tccagtttcc ggacagggaa gagtgggacg agaataaaaa atgtgtatgt tttcccgctc
     7861 tcgtgaatgg tatgcaactg acatgcgcga tctctggcga gagtctggcg tatcgcttta
     7921 ctggagatac gccagaacag tggttagcga gttttcgtca gcatcgctgg gacctggaag
     7981 aagaagcgga aaacttaatt caggaacaaa gtgaagatga tcaaggctgg gtctggttac
     8041 cctgatccag atattcgtcc ttccatttca cgtaattatt cgcggaatag cgtaacccag
     8101 ccttctcttc atcacttaac gggcggatct gtttgacggg gctaccgaga tacagatatc
     8161 cgctctccag ccgtttattt tgtgggacca gactacccgc accaatcatc acatcatctt
     8221 ctactattgc gccatcaagt aaaattgagc ccatcccaac caaaactcga ttgccaatgg
     8281 tgcagccgtg gagcatcacc ttgtgaccaa cagtgacatc ttcgccaatg gttaatgggt
     8341 tgccatctgg gttgtacgag gatttatgag tgacatgcaa catactgcca tcctggatat
     8401 tggtgcgtgc tccgatctgt acataatgta catctccacg aatcacaacg agcggccaga
     8461 tccccacatc atcagccaga cgaacgtcac caatcacgac actgctatcg tcgatcatta
     8521 cgcgctgacc gatttgtgga aaaagatcgc ggtatgggcg taaaacatca gacatactta
     8581 cctcagcaat aaatgattta ctaatgactt tgggggcatt attggccttg tgcaagtctt
     8641 ttagtatgca aaaaagcacc gttttgtgtg cgattgcagc aaaaagggtg aaaaaacaac
     8701 aaacagaaaa aaagatcaaa aaaatacttg tgcaaaatat tgggatccct ataatgcgcc
     8761 tccgttgaga cgacaacgtg aaacacttca caggatggtc ggaacaacga agagaaaaaa
     8821 tcctgaaatt cagggttgac tctgaaagag gaaagcgtaa tatacgccac ctcgcgacag
     8881 tgagctgaaa gccgcgtcgc
//
LOCUS       DAJTNM010000144         8707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTNM010000144 DAJTNM010000000
VERSION     DAJTNM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.144"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(54..2597)
                     /gene="mdoH"
                     /locus_tag="OO172_003570"
     CDS             complement(54..2597)
                     /gene="mdoH"
                     /locus_tag="OO172_003570"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5513598.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2590..4125)
                     /gene="mdoG"
                     /locus_tag="OO172_003571"
     CDS             complement(2590..4125)
                     /gene="mdoG"
                     /locus_tag="OO172_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5513599.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4519..5676
                     /gene="mdoC"
                     /locus_tag="OO172_003572"
     CDS             4519..5676
                     /gene="mdoC"
                     /locus_tag="OO172_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5513600.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5684..7165)
                     /gene="clsC"
                     /locus_tag="OO172_003573"
                     /pseudo
     CDS             complement(5684..7165)
                     /gene="clsC"
                     /locus_tag="OO172_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
     gene            complement(7107..7640)
                     /gene="ymdB"
                     /locus_tag="OO172_003574"
     CDS             complement(7107..7640)
                     /gene="ymdB"
                     /locus_tag="OO172_003574"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5513601.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7735..8046)
                     /locus_tag="OO172_003575"
                     /pseudo
     CDS             complement(7735..8046)
                     /locus_tag="OO172_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8167..8499)
                     /gene="csgC"
                     /locus_tag="OO172_003576"
     CDS             complement(8167..8499)
                     /gene="csgC"
                     /locus_tag="OO172_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5513602.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8558..>8707)
                     /locus_tag="OO172_003577"
     CDS             complement(8558..>8707)
                     /locus_tag="OO172_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5513603.1"
                     /translation="LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNA
                     TAHQY"
BASE COUNT         2181 a         2258 c         2064 g         2204 t
ORIGIN      
        1 attgcaggaa cgatgtaggc ctgataagcg tagcgcatca ggcaactacg tttttattgc
       61 gaagccgcat ccggtttacg caatgccagt ggattgagct ttatcccttc gtaataactc
      121 acccatgaag aatatctttc aggggaattc cagacgcgga aatgcagacg ggccatcgtc
      181 accggatcgc ttagcagcac caggcgacga tcgcgattca gcttctctgg cgtctcgtta
      241 agcgcctgtt caacgtggcg gtcacgggcg atttccagca ccttactggc gcggtgacgc
      301 gctgtcgcca ttgcggttgc cagagcgtta aatgacgggt taaacactgc gtgcataaag
      361 ccatcgtcaa gggagcgttg gcgattcatc tcaaggaacc gatcggtatc aaccagcacc
      421 tgcggcggtg aatactcttc cgggatcagg aacagtttcc agcgtttggt gcgcagacca
      481 acggtggcac ggctggaaat caccgaaaca aacggtgaca ggatcaacga gaagacaatc
      541 ggtgccagcc agaacaggaa acgcagatcc agccacgcca tcccaacagc ccacactaac
      601 cccaacagca gctgtgagcc gtggcgtttg aacgcttcgc cccatgaagt ggagtcatca
      661 tcacgctgcg gtgaattcca caccacttcc cagccaagga acgcgctgac aacgaagacc
      721 gtatggaaca gcatgcgtac cggagccagc agcacggaaa acagcacttc cagcagcaac
      781 gataatgtaa cgcgccagaa gccgccgtat tctttcgttc ctttgcacca gataagcaaa
      841 atgctcaata acttcggcag gaacaacagc accatggtcg aagcaaaaag tgcaatcgcc
      901 agctcaggac gccactgcgg ccacaccggg aacaactgcc gtggttgcag gaagtattgc
      961 ggttcggtca acgcatgcac tacctgcaat gcagtagaga gcgcgaggaa cataaaccac
     1021 agcggagcgg agagataaga catcacgccc gtcaggaaca ccgcacggtg aaccgggtgc
     1081 atacccttaa ccaggaacag acggaagttc atcaggttac cgtggcacca gcggcggtca
     1141 cgttttagct catcaagcaa gttaggcggc aattcttcat aagaacccgg gagatcgtaa
     1201 gcaatccaga ccccccaacc tgcacggcgc atcaacgccg cttctacgaa gtcatgtgac
     1261 aggattgaac cggcaaagga accttcgccc ggcagcggag ccagtgcgca gtgctcgata
     1321 aatggtttca cgcggataat cgcgttatgt ccccagtagt gcgactcgcc aagttgccag
     1381 aagtgcaaac cggctgtaaa cagtggccca tacacgcggg tcgcgaactg ctgacagcgc
     1441 gcatacagcg tatccatacc ggacgctttc ggcgacgact gaatgatccc ggcgttcggg
     1501 ttggcttcca tcaagcgcac caggccgcat aaacaatcac cggtcattac cgagtcagca
     1561 tccagcacca ccatgtagct gtactggctg ccccagcgac ggcagaagtc atcgatatta
     1621 ccgcttttac gcttcacgcg gcgacggcgg cggcgataga aaatctgacc ttcgccaccg
     1681 acttcagcga taagctccat ccaggctttt tgctctgcga cgcagatatc cgggttataa
     1741 ctgtcactca gaatgtagac atcaaagtgt ttggcattcc cggtggcttt tactgattcc
     1801 cacgttgcac gcaagccagc aaaaacacgg ttcacgtctt cgttacagat aggcatgatc
     1861 aacgccgtgc gatgctccgg gtttaatggt tcatcgccaa ctgttgacgc agatatactg
     1921 tatttatcgc gaccaataag cagttgcagg aagcccatca acgccgtcca gaatccggcg
     1981 gacacccaac agaacagtac cgcaaaaagg atcaggatac cggtttgcag catataaggc
     2041 agaagctgca taaaggaaac ccacaaatcc tgaccaacca tatccatagg attaatcagc
     2101 gcccaaccct gataaggaag aatggtcttc atataccagg tcgcgacaac agtttgcgcg
     2161 agcgtcagga tcaacagaat gtaacggcgg atggtaccaa cggtacgcca cttttgctcg
     2221 ctctcctgct cttctttggt caaacgagcc agatagcgcg gcgtgacatc gcgtccacgc
     2281 aggcgatccc agaaacggcc taccgggttg gtacgccacg ggtcgggaaa catcgaggag
     2341 cgttttgctt ctggcatcgc cttaagctga tcgcgccctt cgtcgtcttt aattaactgt
     2401 ccatcagcaa gtgaatctgg ccaggcttgt tccagacgcg cctttaccga gccttgcggg
     2461 gaatcatcct cccgcgccca ggtgcggtgt tcggcatcca gcgcctgatg aacggcgcgg
     2521 atatcagtct tcggcaatgc tgctttctcg cttgcggcga tgggcattgc gtcaatgtac
     2581 tcagttgtct tattcattgg caggtaactg gtagctccag gtttcactca acgtctgatc
     2641 ggcattcacc agcgcagcac gcatttcagt ggttttcttg gcatctttca ctttcacacg
     2701 catcaccaga cgccagcctt tggtaaccgg gttatagcgc accgtgcttt caactatctc
     2761 accattatca ccaatgctgg tttgcgctgt gaccggggta tcctctggca gttttttcat
     2821 ctctgcgccg gtaaaatcga ccacaaaggc gatagtaccg tcaggctggc gaatcaggtt
     2881 cgactgcttc acatcccccg ttgaacgacg cgtttgttgc acccatgcgt tatctggcgc
     2941 atgcagtttg tcttcatcac ggctgaaggt gatggtgtat ttaaagttca tctctttacc
     3001 cggctccggc agctgatccg gcgtccagta agcgacgatg ttatcgttgg tttcatcgtt
     3061 ggttggaatt tccaccagct caacgctgcc tttgccccac tcccctttcg gagtcaccca
     3121 tgcgcttgga cggagatcgt aacgatcatc gagatcttca aagcgggaga aatcacgacc
     3181 tcgctgcaac agaccaaagc ctcgcgggtt ttccatggag aagctgctga ccgctaaatg
     3241 tttcgggtta ttcaacggac gccagatcca ttcgccatta ccagcatgga tagacagacc
     3301 gttagagtcg tgcaactccg gacgatagtt atttgcaggc gacggttggt tcggcccaaa
     3361 caggaacata ctggttaacg gtgcaacccc cagtttgccg actttatcgc gcagatagat
     3421 tttcgactgc acatccacaa ccgtgtcacg ccctggcata accacgaatt tgtaagcacc
     3481 tgtcgcacgc ggcgagtcaa gcaatgcata aatggttaaa cgtttatcag tcggttttgg
     3541 acgctcgatc cagaactctt tgaagcgtgg aaattcttca cccgatggca aagcggtatc
     3601 aattgctagg ccgcgggcag aaaggccata aacctgacct gcaccaatca cgcggaaata
     3661 gctggccccg agcatgctga cgatttcatc gtttttatct ttgctgttga tcgggtaaag
     3721 cactttaaaa ccggcaaaac caaggtcttt taccgtgtct ttgtcatgct gaacatcgcc
     3781 gaaagtgaaa taatccgggc tgtatttgat tcgtttgact gcggtggcag tcacttcatt
     3841 tattttgacc ggggtatcga agtacatacc ctgatggtag aactcgagtt tgaatggggt
     3901 cttcagattg ttccagtacg ctttgtcatg attaaactgg atctgctgat agtccgcgta
     3961 tttcatgtcg cggaaaacgg agggcaagtt gcttttaggc gcctcatagc ctttcccggc
     4021 taaggattga gcttgctttg cgacatcatc aatactgaaa gcccagcttg aagatgtata
     4081 cagggttaac attactgcag cactcaacca acgcattttc atcatttgta gtttatgttt
     4141 cataattagt aagcacttcc ccctttgtgt gcttatatcg atccgatcta ttttaatgga
     4201 taaccagcca ttccgacaac tgaatcccgg tatcgttcag cacgctggct catggattaa
     4261 gcacctgatt gcaaccgttt tcactttccg tgcttctcct aaagacagaa gcatcgactt
     4321 cgtgtagggt ttgccagcaa atttatttat caaccgtctt acagacgctt ccattctggt
     4381 tagtggtagc gatgatgcta tgcataaaaa ccttgaagcc tgcgcctggc gcggtcaggt
     4441 cggtgtcgct atggcaacaa gcctaaaggc ataacccgac agggataaga cgaaaaatcc
     4501 gagattagtt aaccatatat gaatccagta cccgcgcaac gtgaatattt cctcgactcc
     4561 atccgcgcct ggctgatgtt gttagggatc ccttttcata tttctttaat ctattcgagc
     4621 catacatggc atgtgaatag cgccgaaccg tcattgtggc tgaccctttt taatgacttc
     4681 atccactcgt tccgcatgca ggtatttttc gttatatccg gatacttttc ctacatgctt
     4741 tttttacgct atcccttgaa aaaatggtgg aaagtacgtg tcgaacgtgt aggtatcccg
     4801 atgttaacag ccatccccct actgacatta ccgcaattta ttatgctgca atatgtcaaa
     4861 gggaaagcgg aaagttggcc tgggctatca ttgtatgaca aatataatac gttggcctgg
     4921 gaattaatat cacacctgtg gtttttactg gtgttagtag tcatgacgac gctgtgtgta
     4981 tggatattta agcgcatcag aaataattta gaaaattctg ataaaacgaa taaaaaattc
     5041 tcgatggtaa aactatcggt gattttttta tgcctcggca tcggctatgc ggtaataaga
     5101 agaacgattt ttattgtgta tccacccatt ctgagtaatg gcatgttcaa ttttattgtc
     5161 atgcaaacgc tattttattt accgttcttt atcctcggcg cactggcttt cattttccct
     5221 catcttaaag ccttgtttac cacgccgtct cgtggctgta ccctcgctgc agcattggcg
     5281 tttgtcgctt atttactcaa ccagcgctat ggcagtggcg atgcctggat gtacgaaacc
     5341 gagtcggtga tcaccatggt cctcggcctg tggatggtga atgtggtctt ctcctttggc
     5401 caccgtttac ttaacttcca gtcagcgcgg gtgacttatt ttgttaacgc atcgctgttt
     5461 atctatctgg ttcaccaccc gttaacgctg tttttcggcg catacattac accgcacatc
     5521 acctccaact ggcttggttt tctctgtggc ctgatattcg tagtagggat tgcgataatt
     5581 ctgtatgaaa ttcatttacg catcccgtta ctgaagtttt tgttctctgg taaaccggtt
     5641 gttaagcgtg agaacgataa agcaccagcc cgttaagcca catttacaat aaccattcca
     5701 cgggcaatat cgacgccagt ctgaccataa cccgcttcca gaaactggtg gcgggttctt
     5761 ttttgagaat aacctctttc tttgcatgac gatcaaccca gttgatccgt ccccacctgt
     5821 ccagacggag ctgccaggcc gcatcgtact ggctctgaat aaagcgttta tcaattaact
     5881 gtgccagcgt ttcgctctct atcacgaagc ccatttcagt attgagcaat gttgaacgcg
     5941 gatcgaaatt gaaagagccg ataaacaccg ttttaccatc gatgctaaaa gttttagcat
     6001 gcaggctggc accggaatta ccggttatgc cgcgatcgtg taacgtactg ctttgttcac
     6061 gcgtcggctt gagttcatat aattccacgc catagcggag caattttttg cgccagcgcg
     6121 cgtatccggc atggacgaca gcaacatcgt tagcggcaag agaattggtt aggatcgcaa
     6181 tctttacccc ttttctcacc atccgtaaga gttgcgccac acctgcgcgt gtcggtacaa
     6241 aataggaaga gataatatcg atgcgttcac tgggtgagcc catgatatcg aacaggcgct
     6301 gcggtaacag tgaatgccgt tttgccttgc cctccccttt cgccggatca tcacttaata
     6361 aacgtgtctt cgcccagata agcggcaacg ttccatcaac cagatgattt ataaatggac
     6421 tggattccat tttgcgtaaa taacgatgcg tcatggcatc gttatgccag gaggcgggta
     6481 actcgatgcg atccgccatt tcaccttccg ggacatccag cacctgctgt aagggtgaaa
     6541 ccgatttgca ataccagtag cgggcgaaat catcggcaac gtcctctacc acggggccta
     6601 ttgccatgac atctaaatcc gaaaaaagtc gctcttcccc tgcactaaaa taggcatcac
     6661 caatatttag tcctcccact agggtaacca cgccatcgac agtgaaactt ttattgtgca
     6721 tccggcgatt gagacgggaa aagtcggtga tatatccaag cggacgtagc aagcgaaacg
     6781 agaaaggatt aaaaagccgg acttcaatgc gtggatgact gtcaagcaag cgtaaaatgt
     6841 cgtcaagtcc gggcgtattg ttgtcgtcca gcaacaaacg gacgcgaacg ccacgctttg
     6901 ctgcggctaa cagggcggaa aacagtaacc gccccgacat atcgtcctac cagatgtaat
     6961 actgaacatc cagcgtatgc tctgccattt cggccagacg ataccgggcg gcaaacgcat
     7021 ccagactttt ctccagaggg aaaagaccac actgaccggg atgttgcgaa cacagtggca
     7081 gcaccgcgct cgccagccgg ggcaaatcat tcatctcctt gttgggtaag gagtctttcg
     7141 tagaggtggg cgttttcttc atcataacag acaaagtata cctgttcggg taaagcgtga
     7201 cgggtaataa attctgaaac ggttttaact gcgatttcag ccgctgccgc acgagggtaa
     7261 ccataaaccc cagtactgat tgcaggaaaa gccactgacg tatagctgtt tgccgccacc
     7321 agtcgtaggc tattcagata ggcatcctgc aaaagctggt cttcgttttg ttcaccacca
     7381 cgccagactg gcccgacggt gtgtactacg gctttagcgg gaagatcgcc tgcaagcgta
     7441 ataacggcat gccccgtagg gcaatcgccc tgctgttgcc tgacttttaa acaggcatcc
     7501 agcagggccg gacccgctgc gcgatgaatg gccccatcga cgccgccgcc tcccattaat
     7561 gacggattag ccgcattcac aatcacatca acggccagtt tggtaatatc accctgcaca
     7621 acatgaatac gcgttttcat aacgcctcct tacaccttct tgtttcttaa gtgtacagca
     7681 ggctttttgg ttaaatcgca tttttctgct cagcaacgtg agttgttcag aaacttagtt
     7741 atattcgatg ttcattacag ccagcttttt ctgtttatca agatactgca cttttactga
     7801 ggctatttgc gcgtttttga cattgccgct cattaacgct ttgctggagt aggttttgcc
     7861 cggaatagaa ccattctatg ggcagttcat ttctacgggt gatgactggg tgctgacgtc
     7921 acatgccggt tccacaattt ggccataaaa atggatcacg cccccttgcg cggtgcttcc
     7981 tgcaatggca ataaaaggaa aaaacaaact gccgagcata agagagtcta aaaaaggtct
     8041 gaacatttat tctatttcct caatgattag tcatccttga gggttgtgtt atccatactt
     8101 tcaggttggc tgattcgcag cagaccattc tctccagatt catcttatgc tcgatatttc
     8161 aacaaattaa gacttttctg aagagggcgt ccattgttgt gataaatgaa gtgcctgtcc
     8221 atcagaaaca gtaacaacta ttttcacccg atcttccggg gaaatattta aactcaactt
     8281 cgtcaaagca atgggttgat tagcaggcaa tgagagggtc ttttcttgct tcgtctgact
     8341 ttgccctgaa ctgccttcgc gcagggacaa tatttgtact ctgcacagac aagattgagt
     8401 aagagtaact tcaggaataa tggtgtacat atccccttgc tgggtcgtat taaaggttat
     8461 ctggctggaa agtgccgcaa ggagtaataa cgcattcata ttcttctccc gaaaaaaaac
     8521 agggcttgcg ccctgtttct gtaatacaaa tgatgtatta atactgatga gcggtcgcgt
     8581 tgttaccaaa gccaacctga gtcacgttga cggaggagtt cgatgcagtc tggtcaactg
     8641 cagcaccgtt gccaccaccg aactgtttaa cagtcatttc agaatttttg ccgttccact
     8701 gatcaag
//
LOCUS       DAJTNM010000145         8534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTNM010000145 DAJTNM010000000
VERSION     DAJTNM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.145"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(424..630)
                     /locus_tag="OO172_003578"
     CDS             complement(424..630)
                     /locus_tag="OO172_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5513604.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            943..1032
                     /gene="kdpF"
                     /locus_tag="OO172_003579"
     CDS             943..1032
                     /gene="kdpF"
                     /locus_tag="OO172_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5513605.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1032..2705
                     /gene="kdpA"
                     /locus_tag="OO172_003580"
                     /pseudo
     CDS             1032..2705
                     /gene="kdpA"
                     /locus_tag="OO172_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            2728..4775
                     /gene="kdpB"
                     /locus_tag="OO172_003581"
                     /pseudo
     CDS             2728..4775
                     /gene="kdpB"
                     /locus_tag="OO172_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4784..5356
                     /gene="kdpC"
                     /locus_tag="OO172_003582"
     CDS             4784..5356
                     /gene="kdpC"
                     /locus_tag="OO172_003582"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5513606.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAVAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5349..6332
                     /locus_tag="OO172_003583"
                     /pseudo
     CDS             5349..6332
                     /locus_tag="OO172_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            6332..8028
                     /locus_tag="OO172_003584"
                     /pseudo
     CDS             6332..8028
                     /locus_tag="OO172_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
     gene            8025..>8534
                     /gene="kdpE"
                     /locus_tag="OO172_003585"
     CDS             8025..>8534
                     /gene="kdpE"
                     /locus_tag="OO172_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR5513607.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKV"
BASE COUNT         1828 a         2192 c         2453 g         2061 t
ORIGIN      
        1 attggattag atatgttaac aattaattat tcttaatata tcaataaagt atatattact
       61 ctctttacta tctgttttta ttgcggtagc gatactaata acaaaattcg gaatttattt
      121 agatgctgga attagaaaaa gggatgacgg ctttctcacc ttaccctgaa gtttcacgaa
      181 agtgtgtaag gaagtatgca atctaactgg agatttctga tgatctagtc gcttagagcg
      241 acaatgaagg tgaccgcatc ctgggtagcc ctatgaaagg atactattga aacgtgatgc
      301 taaaacagtg atgaaatgaa taatacgatg gaaacgtctt ccatgttaat tatcatggaa
      361 gacgttgatg ttgaacacct aaaccagccc cctgatacga ggctggctta agcgcgcagg
      421 caatcagtaa aaatcaccgg ttgccttttc ggcctgatcc atccacaccg gtttatcgct
      481 ggttttcgac cagacgcggt gcaggtagct gtaaaagcgg gcgcggtctt tccagaaaaa
      541 catgaaaggc agcgccagaa cgcccgctgc aaccgcatat gtacggcgta aaaagataag
      601 ccatgcagga tattctctgt agagttccat acttttctcc cccttttgta tctacccggt
      661 gaatagcacc ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc ttttgtgtaa
      721 ttttaccact catccgacca cttatttttg cttattgatg gtttatttac attcacactg
      781 taattaaatt acacaaaagt taaattaata ctaaccatca gttaaatcat ggcctttgcc
      841 atttttatac tttttttaca ccccgtccgc agatttttgc gaaatctttg cagccagaat
      901 tctactcttc cggtatcact tttaggctac tggaggtgca ctgtgagtgc aggcgtgata
      961 gccggcgtgt tgctggtgtt tttattactg ggttatttgg tttatgccct gatcaatgcg
     1021 gaggcgttct gatggctgcg caagggttct tactgattgc cacgttttta ctggtattaa
     1081 tggtgctggc gcgtccttta ggcagcgggc tggcgcggct gattaatgac attcctcttc
     1141 caggtacagc gggcgttgaa cgtatactct ttcgtttacc tggcgtctct gaccatgaga
     1201 tgaactggaa gcaatatctt tgtgccattc tcggcctgaa catgctgggg ctggcggtgc
     1261 tgttttttat gttgctcggt cagcactatc tgccgcttaa tccatagcag ttgccggggc
     1321 tgtcgtggga tctggcgctg aataccgccg tcagctttgt cacgaatacc aactggcaat
     1381 cttatagcgg tgaaaccacg ttgagctatt tcagccagat ggcaggctta acggtacaaa
     1441 actttctttc tgccgccagc gggattgcgg tgatttttgc cttcatccgt gcgtttactc
     1501 gccagagcat gagcacactc gggaatgcct gggtcgatct gctacgcatc acgttatggg
     1561 tgctagtccc tgtggcgttg ttaatcgcac tgttttttat tcaacaaggt gcgctgcaaa
     1621 actttctgcc ctatcaggca gtgaataccg ttgaaggagc gcaacagctg ttacccatgg
     1681 gaccagtagc ttctcaggaa gcgatcaaga tgctcggtac taacggcggt ggcttcttta
     1741 atgccaactc gtcgcatccg tttgaaaacc caaccgcact gaccaacttc gtgcagatgc
     1801 tggcgatctt cttgatccca acagcgctgt gctttgcctt tggtgaagtg acgggcgatc
     1861 gccgccaggg gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc tgcgtaggcg
     1921 tggttatgtg ggcagaagtt cagggtaatc ctcatctgct ggcgctcggc gcagacagca
     1981 gcatcaatat ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc ctgtttgcgg
     2041 tcgtgactac ggcggcgtcc tgtggcgcgg tgattgcgat gcatgattcg tttaccgctc
     2101 tcggcggcat ggtgccgatg tggctaatgc aaattggtga agtggtgttc ggcggtgtcg
     2161 gttctggtct ttacggcatg atgctgtttg tcctgctggc ggtgtttatt gccgggctga
     2221 tgattggtcg tacaccggaa tatctgggta aaaaaatcga cgtacgcgag atgaaactga
     2281 ctgcactggc gattctggtt actccaacac tggtactgat gggcgcggcg ttggcgatga
     2341 tgaccgacgc cggacgtagc gccatactta accctagccc gcatggtttt agcgaagtgc
     2401 tgtacgccgt gtcgtccgcc gctaacaaca acggcagcgc ctttgccgga ttaagcgcca
     2461 actctccgtt ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc tccggggtga
     2521 ttatcccggt gatggcaatt gccggttcgc tggtgagtaa aaagagccaa cccgccagct
     2581 ccggcacgct gccaacgcac ggcccgctgt ttgttggcct gttaatcggc accgtgttgc
     2641 tggttggcgc actgaccttt atccctgccc tggcgcttgg tccggtggcg gaatatctct
     2701 cctgatgata ttgagtgagc actgaatatg agtcgtaaac aactggcgct attcgaacca
     2761 acacttgtcg ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca ggcgcaatgg
     2821 cgcaatccgg tgatgtttat cgtctggatc ggcagtctgc tgaccacctg tattagcatc
     2881 gcgatggcaa gcggtgcgat gcctggcaat gcgctgttta gcgcggcaat tagcggctgg
     2941 ctgtgggtca ccgtactgtt cgctaatttc gccgaggcgc tggcagaagg ccgcagtaaa
     3001 gcgcaggcca acagtcttaa aggggtgaaa aaaactgcct ttgcccgcaa gctgcgtgag
     3061 ccgaaatatg gcgctgcggc ggacaaagtt cctgccgatc aactgcgtaa aggtgacatc
     3121 gtgctggtag aagctggcga tattatcccc tgcgatggtg aagtcattga aggcggcgca
     3181 tcggtcgatg aaagcgccat caccggggaa tcggcaccgg tgatccgtga atccggcggc
     3241 gattttgcct ccgtcaccgg cggcacgcgt attctttctg actggctggt gattgagtgt
     3301 agcgttaacc ccggtgagac atttctggat cggatgatcg cgatggtgga aggcgcacag
     3361 cgacgcaaaa caccgaacga gattgccctg actatcctgc tgattgctct gaccatcgtc
     3421 tttttactgg caaccgccac gctgtggccg ttttccgcgt ggggcggtaa tgccgtgagt
     3481 gtaacggtac tggtggcgct gctggtctgt ctgatcccaa ccactattgg tggcctgttg
     3541 tcagcgatcg gcgtcgccgg gatgagtcgg atgctgggcg cgaatgtgat tgccaccagc
     3601 ggacgtgcag ttgaagcggc aggtgacgtt gacgttctgc tactggataa aaccggcacc
     3661 atcacactcg gtaaccgtca ggcgtcggag tttatccccg cgcagggcgt ggatgaaaaa
     3721 acgctggctg acgccgcaca actggcttcg ctggctgatg aaacgccgga aggtcgcagt
     3781 attgtgatcc tcgccaagca acgttttaac ctgcgcgagc gcgatgtgca gtcgctccat
     3841 gccacctttg taccgtttac tgcgcaaagc cggatgagcg ggatcaacat cgacaaccgc
     3901 atgatccgta aaggttctgt cgatgccatt cgccgccata ttgaggctaa cggcggtcac
     3961 ttccctgccg atgttgatca aaaagtcgat caggttgcgc gtcaaggagc cacgccgctg
     4021 gtggtggtgg aaggttctcg tgtgctgggc gttattgcgc tgaaagatat cgtcaaaggc
     4081 ggtattaaag agcgcttcgc ccagctgcgc aaatgggcat taaaacggtg atgattaccg
     4141 gcgataaccg tctgactgcc gccgcgatag ctgcggaagc gggtgtcgat gattttctcg
     4201 ccgaagcgac accggaggcc aagctggcat tgatccgtca gtatcaggcg gaaggtcgtt
     4261 tggtagcgat gaccggcgac agcaccaacg atgctccggc gctggcgcag gcagatgtcg
     4321 cggtggcgat gaactccggc acccaggcgg cgaaagaggc gggcaatatg gtggatctcg
     4381 actctaaccc gaccaagttg atcgaggtgg tgcacattgg caaacagatg ctgatgaccc
     4441 gtggctcgct gaccaccttc agcattgcca acgatgtggc gaaatacttc accattattc
     4501 cggcggcgtt cgcggcaaca tatccgcagt taaatgcgct gaacatcatg cgcctgcatt
     4561 cgcccgactc cgccattctc agtgcggtca ttttcaacgc cttgattatc gtctttttga
     4621 ttcctctggc gttaaaaggc gtgagttata aaccgcttac cgcttctgcc atgttgcgcc
     4681 gtaacttatg gatttacggt ctgggtgggc tgctggtgcc gtttattggt atcaaagtca
     4741 ttgatttact gctaaccgtt tgtagtctgg tgtgaggttt accatgagtg gattacgtcc
     4801 ggcattatcg acatttctct ttctgttatt gattactggc ggcgtttacc cgctgctgac
     4861 caccgcactg gggcaatggt ggtatccctg gcaggccaac ggttcgttga ttcgtgaagg
     4921 tgatacggtg cgcggttcgg cattaatcgg gcaaaatttt accggcaacg gctattttca
     4981 tggtcgcccg tcggcaacag cagaaatgcc ctataatcca caggcttctg gcgggagcaa
     5041 tctggcagtc agtaacccgg agctggataa acagatagcc gcacgcgttg ccgcattacg
     5101 ggccgctaac ccggacgcca gcacgaacgt tccggttgaa ctggtgacgg cgtcggcaag
     5161 cggactggac aataacatca ccccgcaagc ggtggcctgg cagatcccac gcatcgcaaa
     5221 agcgcgtaat ctcagcgttg aacagctcac gcagctgatc gcaaaataca gccaacaacc
     5281 gctggtgaaa tatatcggcc agccggtcgt caacattgtc gaactcaatc tggcactgga
     5341 taagcttgat gaataacgaa cccttacgtc ccgaccccga ccgtctgctg gaacaaactg
     5401 ccgcgccgca tcgggggaag ctgaaagttt tcttcagtgc ctgtgcaggc gtcgggaaga
     5461 cctgggcgat gctggcagaa gcccagcgac tgcgggcgca agggctggat attgtggttg
     5521 gtgtggtaga aacccatggg cggaaagata ccgccgccat gcttgaaggg ctggctattc
     5581 tgcctctgaa acgccaggcg taccgtgggc ggcatatcag cgagtttgat ctcgatgccg
     5641 ccctcgcccg ccgtccggcg ctgatcttaa tggacgaact ggcgcacagt aatgcgccag
     5701 gttctcgtca tcctaaacgc tggcaggata tcgaagaact gctggaagct ggcattgatg
     5761 ttttcacaac cgtcaacgtt cagcatctgg aaagtctgaa tgatgtggtc agcggcgtca
     5821 ccggaattca ggtacgggaa accgtgcccg atcctttttt cgatgccgcc gacgacgtgg
     5881 tgctggtgga cttgcccccg gacgatctgc gccagcggct gaaagaaggc aacgtctata
     5941 ttgccgggca ggcggagcgc gccattgaac attttttccg caaaggcaat ctgatcgccc
     6001 tgcgcgaact ggcactgcgc cgtactgccg atcgcgttga tgagcaaatg cgcgcctggc
     6061 gggggcatcc tggcgaagag aaagtctggc acacgcgcga cgcgatcctt ttatgcatcg
     6121 gccataacac cggcagcgaa aactggtccg cgcggcggcg cggctggcgt cacggctggg
     6181 gagcgtctgg cacgcggtgt atgttgaacc ccctgccctg catcgcttac cggagaaaaa
     6241 acgtcgggca attctcagcg ccttacgtct ggcgcaggag ctgggcgcgg agacggcaac
     6301 actttctgat ccagcagaag agaaagcggt agtgcgttat gcccgtgaac ataatctcgg
     6361 caagattatt ctcggtcgcc cagcctcgcg ccgctggtgg cgtcgggaaa catttgctga
     6421 ccgactggcg cgcatcgccc ccgatctcga tcaggtgctg gtcgcgcttg atgaaccacc
     6481 cgcccgcacg attaacaacg cgccggatag ccgctcattt aaagacaagt ggcgggtaca
     6541 gattcaggga tgcgtggttg ccgccgcgtt atgcgccgtt atcaccttaa ttgccatgca
     6601 gtggctgatg gcgtttgatg ccgccaacct ggtgatgctg tatctgcttg gcgtggtggt
     6661 ggcgctattt tatggacgct ggccttcggt ggttgccacc gtcattaatg tggtgagttt
     6721 cgatctcttt tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc aatatctgct
     6781 gaccttcgcg gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg ctggcgtgcg
     6841 ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat atgaaatgtc
     6901 gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc gccaccagcg aacaatttat
     6961 tgcctctacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg gtaaattgca
     7021 gccgttaaca catccgcaag gaatgacgcc atgggacgat gccatcgcgc agtggagttt
     7081 tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac cgtaccagat
     7141 tttgccgcta aaaagcggcg agaaaaccta cggctggtgg tggtggagcc ggggaatctg
     7201 cgccagttga tgatcccgga acagcagcgc ctgctggaga cttttacgct gttagtcgcc
     7261 aatgcccttg agcggctgac gctaaccgcc agcgaagaac aggcgcggat ggcaagcgaa
     7321 cgtgaacaga tccgcaacgc cctgctggca gcgctttcgc atgatttacg cacgccgctt
     7381 acggtgctgt ttggtcaggc agaaatctta acgctcgatc tggcaagcga aggatcaccc
     7441 cacgcccgcc aggccagcga gattcgtcag catgtgctga acactacccg actggtgaat
     7501 aatctactgg atatggcgcg aattcagtcc ggcggcttta atttgaagaa agagtggtta
     7561 acgctggaag aagtagtcgg cagcgcgctg caaatgctgg aaccgggttt atcgtcgccc
     7621 atcaatcttt ctctgccaga accgctgacc ttaatccacg ttgacgggcc actctttgaa
     7681 cgggtgctga ttaatctgct ggagaacgcg gtgaaatatg cgggtgcgca ggccgaaatt
     7741 ggtatcgatg cccacgttga gggcgaaaat ctacaactgg atgtctggga taacggcccc
     7801 ggtcttccgc caggccagga gcagacgata tttgataagt ttgctcgcgg gaataaagag
     7861 tcggcggtgc cgggtgtcgg gcttgggttg gcaatttgtc gggcgattgt ggatgtacac
     7921 ggcggcacca ttaccgcgtt caaccgaccg gaaggtggtg cctgttttcg tgttacactt
     7981 ccccagcaaa ccgctcctga acttgaagac tttcatgagg atatgtgaca aacgttctga
     8041 ttgttgaaga tgaacaggct attcgtagct ttctgcgcac ggcgctggag ggcgacggga
     8101 tgcgcgtctt tgaggccgaa actctgcaac gcggcttgct ggaagcggca acccgtaagc
     8161 cagatttgat tattctcgat ctcggcctgc ccgatggtga tgggattgag tttatccgcg
     8221 acctgcgcca gtggagcccg gtgccggtga ttgtgctttc cgcacgcagc gaagagagcg
     8281 acaagatcgc cgcgctggat gccggagcgg atgattatct gagtaagccg tttggcattg
     8341 gcgaattgca ggcccgtctg cgcgtcgctt tacgccgcca ctccgccacc gccgcgcccg
     8401 atccgctggt gaaattttcc gatgttaccg tcgatttagc cgcccgcgtg attcaccggg
     8461 gtgaggaaga ggtgcatctc acaccaattg agttccgcct gctagcggtg ctgctcaaca
     8521 atgccggaaa agta
//
LOCUS       DAJTNM010000146         8476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTNM010000146 DAJTNM010000000
VERSION     DAJTNM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.146"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..313)
                     /locus_tag="OO172_003586"
     CDS             complement(<1..313)
                     /locus_tag="OO172_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5513608.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NF"
     gene            complement(323..871)
                     /locus_tag="OO172_003587"
     CDS             complement(323..871)
                     /locus_tag="OO172_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5513609.1"
                     /translation="MGLPPLSKTPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYTEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(871..1578)
                     /locus_tag="OO172_003588"
     CDS             complement(871..1578)
                     /locus_tag="OO172_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5513610.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1593..2270)
                     /gene="ydjY"
                     /locus_tag="OO172_003589"
     CDS             complement(1593..2270)
                     /gene="ydjY"
                     /locus_tag="OO172_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5513611.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2275..2985)
                     /locus_tag="OO172_003590"
     CDS             complement(2275..2985)
                     /locus_tag="OO172_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5513612.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3152..3958)
                     /gene="xthA"
                     /locus_tag="OO172_003591"
     CDS             complement(3152..3958)
                     /gene="xthA"
                     /locus_tag="OO172_003591"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5513613.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4404..4982
                     /locus_tag="OO172_003592"
     CDS             4404..4982
                     /locus_tag="OO172_003592"
                     /inference="COORDINATES: protein motif:HMM:NF012428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5513614.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGSIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDATCAVIVEPI"
     gene            4998..5624
                     /locus_tag="OO172_003593"
     CDS             4998..5624
                     /locus_tag="OO172_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5513615.1"
                     /translation="MPASNAFLQGLRELCDRHNALLIFDEVQTGVGRTGELYAYMHYG
                     VTPDLLTTAKALGGGFPVGALLATEECARVMTVGTHGTTYGGNPLASAVAGKVLELIN
                     TPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRGLGLLIGCVLNADYAGQAKQISLEA
                     AKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRFAAACEHFVSRGSS"
     gene            5621..7354
                     /gene="astA"
                     /locus_tag="OO172_003594"
     CDS             5621..7354
                     /gene="astA"
                     /locus_tag="OO172_003594"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5513616.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAAGWLAAGRETGQALSALEDLDGLLFTGSANTGYQLHRQLSGQPEKILALEMGGNNP
                     LIIDEVADIDAAVHLTIQSAFVTAGQRCTCARRLLLKSGAQGDAFLARLVAVSQRLTP
                     GNWDDEPQPFIGGLISEQAAQQVVTAWQQLEAMGGRTLLAPRLLQAGTSLLTPGIIEM
                     TGVGGVPDEEVFGPLLRVWRYDTFDEAIRMANNTRFGLFCGLVSPEREKFDQLLLEAR
                     AGIVNWNKPLTGAASTAPFGGIGASGNHRPSAWYAADYCAWPMASLESDSLTLPATLN
                     PGLDFSDEVVR"
     gene            7351..>8476
                     /gene="astB"
                     /locus_tag="OO172_003595"
     CDS             7351..>8476
                     /gene="astB"
                     /locus_tag="OO172_003595"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR5513617.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEE"
BASE COUNT         1985 a         2180 c         2316 g         1995 t
ORIGIN      
        1 ggaagttttc gccgttcacc cagagcagag ccaccgagcc gcccgtttta cgaccggcag
       61 cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga acaatcttca
      121 ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag cggttaattg
      181 cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca tttttaattt
      241 cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc agcaaccacc
      301 cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac agcccctgag
      361 cgggaatgtc catggcagaa ttaaaacgag ccgacaggtt ttgcaggccg atcaatgccg
      421 tcagttcggt gagcgcctga gcgtcaaaat gcgcagccag tcgggtacgc agggcatcat
      481 cgactgttgg cggtgtgacg cttgcggctt cggtgtactc cagcgccagc cgttcttcat
      541 cgctaaagag cgggctttgc cgccagtcag ccactgccag cagtttatcg gtgctgccgg
      601 tacgctcggc gactttcata ctggtgatat ccacgcaaaa ctcacacagg cacatttgcg
      661 caatgcgcgc gctgacaagc gatcgcacta ccggatcgag cggtgagcgt ttgcgctcca
      721 gccacccaac aaacattgac accaaataaa agataaacgg gatccgcccc caccagcgaa
      781 tcgggcttag cacctcgccg taatggcgac gatgcagcca cgcctgcgga cgtaaaatga
      841 aaggagtttt gctaagcggc ggtaaaccca tcagtcattc ctcttctggc gctcaagcca
      901 tattttcttc gccataaaaa tcaccacggt cagggcaaac agaataaaca gttcggtgac
      961 aaaccagaaa gtaccgcctg ttaacatgct gcccgcccag gaataaacaa tagtcgccgg
     1021 taattgacca agcccggtgg cgataaaaaa cgagcgaaaa cgtattgaag tcagaccggc
     1081 agcatagctg attggatcga aagggacaaa aggcaataac cgacagacca ggatggtgtg
     1141 tttgccgtag cgagtgaaaa agccgtccat actgtcaagc acggttttgc cggttaattt
     1201 ttccaccact tcgcggccca tcactctggc gataaaaaag cacagcgccg cgccggccat
     1261 cgaactggtc cacgacagca agccgcccca gaacgcgcca aacagcgacg cattggcaaa
     1321 ggtgatcaaa aacgcaggta gcggtgcagc aatggcctgt aaaatcatca acaagaacga
     1381 gacaactgcc gccagtgcgc cgtaagactg aataaagcgt tctataccct gttggtccac
     1441 ggcggcaaac gccgcaacgc tgcgattgat aaactcatgg acgccgggaa gcagcgccca
     1501 tgcgagcagc atagcgaaca acaggatgat gagggttata cggtagtacc agattttacg
     1561 cgattgtatc ttcatcatcc tttatcctgg ctttattcgg tgattttaaa ggtaaccgtt
     1621 gccaacgtgt tatccgccgg gagaactgag gcattgcctt tgaatttcac ttcgccgcgt
     1681 ttttccaccg caccataagt gtatgttgca ttgctgacga tgccgaccgg gcagctatcc
     1741 agacacacca ggcaacccgt tttcttctct tctgctgccg ttaaattacc gccaaagcgc
     1801 atgtccagtt tcttgccatt actgtcaaca atcacttcat cgaaggaata cgcttttgcc
     1861 gccccttgcc agttgaccga aatatccagt ttgctgcctg tgacatgagt cgtttcttta
     1921 ttatccatcg tcatgttttc gcccggcgta ccacctgcct ctttcagggc ttcataaaat
     1981 gctttcgggg tcgcataagc cataaacagc gatttatgtc cgttggagcc atctttaaac
     2041 acaataccgt gacgagtgtc gtcggtgagg aaacgaccat taatttgcac cagcatggtt
     2101 accgtatggt tattggcatc gacgcgcagc ggttggctgt tcgaaagtcc gtcgtattcc
     2161 acttttgccg ccgcattctc tttttgatcg cagccgctaa gcccggcaac agccagtaag
     2221 cagagtgcag ccagtccttt tttccatgac actgaataat gttgcgacat cgttctatcc
     2281 ttcatttttt gggtgagtaa gtgggctgcg gcgcgaagca gacagatcca catgtttgtg
     2341 tcgggcgtag agttttgcga gctggatgag aataaacagc gccaggcccg ccagacagag
     2401 ttgtaaaata aagcgcagcg taatgccttc attggcgaga tcgcttgcca tcacggtata
     2461 aataacaata ccgggcaggg tcgtgagtgc cgaaataagg gtataaggcc agaaggcgat
     2521 ggtggttaat ccgtaagcgt aattttgaat attgtaagga aacaacggga ttaagcgggt
     2581 cagaataaga aaatcaatac cgttacgcgc aatgcctttt tcaatggcct ggaaggtatg
     2641 gctatggccc acgtatttca gcagtaaatc acgtcccagc cagcgcgcca gcaggaatga
     2701 gcacgaggag gccagcgttg cggcaattaa tgagagtagt gtccctaaga gcgggccaaa
     2761 aactattcct ccagcgatca ccagtatgct tcctggtaac agaaagaggg gggcattgat
     2821 gaataacaga atatagaggt tataaccgaa caatccgctc tggcgaatga gtgtctgtaa
     2881 gtggggtaaa tcggtgagca gatcgaataa accgaaagcg tggatagcgt aaatgaccag
     2941 cgcaaaaata agacaggcaa acagaaagtt acgctcagcg ttcatcgtgg agtttcctat
     3001 atcaaacttg ataatagaga agtggtcaga caactaaaaa cgcagtgacg gtataacaaa
     3061 ggacggcagg caacaaatca ttactgacag tttgttgatt tttctcaagg ttaattctcc
     3121 tgacccagtt tgagccagga gagctgctaa attagcggcg gaaggtcgcc cagacggggg
     3181 cgtgatcgga cggtttttcc atgctgcgaa tttcatagtc gatgccggtt tctacgcaac
     3241 attctgccag cggttggctg gcgagcagca ggtcgatgcg cagaccacgg ttatcgtcaa
     3301 aaccttttga gcggtaatca aaccatgaga aacgatctgc tgtttgcgga ttagcatggc
     3361 ggaaggtatc gaccaccccc cagctcatca gcctgtccat ccattcgcgc tcttccggca
     3421 ggaaagagca tttaccggta cgcagccagc gcttacggtt ttcttcgcca atgccgatat
     3481 ccagatctgt agggctgata ttcatatcgc ccataatcag taccggatta tcacgtttga
     3541 gttcggtttc caggtagttt tgcagattct gataaaactg cgcttttgcc gggaatttta
     3601 tcggatggtc gcggctttca ccctgcggga agtaaccgtt gatcacggtg acataaccca
     3661 gcagtgaggg gatttccgcc ataataatcc gccgctgcgc ctcttcgtcg tcaccgggaa
     3721 agccgcgacg cacggcaatc ggcgtctctt tggtcagcag cgccacgcca taatggcctt
     3781 tctgcccgtg ataaaacacg ttgtagccga gcttcgccac ctcttcgagc ggaaacatat
     3841 cgtcatgaac ttttgtctcc tgcaggccaa tcacatccgg ttggtgcttt tcgacgatgg
     3901 cttcaagctg gtgaggtctg gcgcgcaggc cgttgatatt aaaagagaca aatttcatag
     3961 tcgctgccat ttattcagat aaagagtgcg tggatggtag cagaatttcg cgttgcttac
     4021 cgcctgttgt taagtgattt gatcgatggt tgttaatgat gtcaacgatg gcgcaaaaaa
     4081 tgcccgcttt tggtgcgcgc tgcgtcagaa tggcgcagta atttccagta aatttcgtca
     4141 atgatcacaa ttccagaatt atattttact tctgcataac attgcgtttt ttattctttt
     4201 tattcaccca catcgcaaac gtattcactt tatatgcact ttaaatgcat atggtttggt
     4261 tataactcct tgatttccag ttagcctccg ccgtttatgc acttttatca ctggctgaca
     4321 cgaaccctgc aatctacatt tacagcgcaa acattactta ttgttaacat acaaataacg
     4381 aattatttac tgtagaggtc gctatgtctc agccaattac gcgtgaaaac tttgatgaat
     4441 ggatgatacc tgtttacgct ccggcaccct ttataccggt acgtggcgaa ggttcgcgct
     4501 tgtgggatca gcaggggaaa gagtatatcg acttcgcggg tagcattgcg gtgaacgcgc
     4561 tgggccatgc gcatccggaa ctgcgtgaag cgctgaacga acaggcgagt aagttctggc
     4621 ataccggcaa cggttacacc aacgagccgg tactgcgact ggcgaaaaaa ttgatcgacg
     4681 ccacgtttgc cgatcgcgtc ttcttttgta actccggtgc ggaagccaac gaagcggcgc
     4741 taaaactggc gcgtaaattc gctcacgacc gctacggcag ccataagagt ggcatcgtgg
     4801 cgttcaaaaa tgcgtttcat ggtcgcacgc tgtttactgt cagtgcgggt gggcagccag
     4861 cctattcaca ggattttgcg ccactgccgg cggatattcg tcatgctgca tataacgata
     4921 ttaactctgc cagcgcgctg attgacgacg ccacctgtgc ggtgattgtc gagcccatct
     4981 agggcgaagg cggtgtggtg ccagccagca acgcgttttt acaaggtctg cgtgaattgt
     5041 gtgaccgcca caatgccttg ctgatttttg atgaagtaca aaccggcgtc gggcgcactg
     5101 gtgagctgta tgcctatatg cactacggcg tgacgcccga tctgttaacc accgccaaag
     5161 cgctgggcgg cggtttcccg gtcggtgcgt tgttggcaac cgaagagtgc gcccgcgtga
     5221 tgaccgttgg cactcatggc accacctatg gcggtaatcc gctggcctcg gcggtagcag
     5281 gcaaagtgct ggagctcatc aacacaccag agatgcttaa tggcgttaaa cagcgtcacg
     5341 actggtttgt tgagcgtctt aatactatta atcaccgcta tggtttgttc agtgaagttc
     5401 gcggcttagg tttgttgatt ggctgtgtac tgaatgccga ttacgccggg caagcgaaac
     5461 agatctctct ggaagcggcg aaagcaggcg tgatggtact gattgcgggt ggcaacgtgg
     5521 tgcgttttgc gcctgcgctc aatgtcagcg aagaagaggt gacgaccgga ctggatcgct
     5581 ttgcagctgc ttgcgaacac tttgttagcc gaggttcatc atgatggtca tccgtcccgt
     5641 tgagcgctca gatgtctcgg cgctgatgca gcttgccagc aaaacgggcg gcggcctgac
     5701 gtcgcttccc gccaatgaag ccacgctttc ggcgcgtatc gaaagggcaa tcaaaacctg
     5761 gcaaggcgaa ctgcctaaaa gcgagcaggg ctatgtgttc gtgctggaag atagcgagac
     5821 aggcaccgtg gcggggattt gcgctattga ggtggcggtt gggctgaacg atccctggta
     5881 caactatcgc gtcggcacgt tggttcacgc ctcaaaagag ctgaatgtct ataacgcatt
     5941 gccgacgctg tttcttagta acgatcacac cggcagcagc gagctgtgca cgctgtttct
     6001 cgacccggac tggcgcaaag agggcaacgg ctatttgctg tcgaaatcgc gctttatgtt
     6061 tatggcggct tttcgcgaca agtttaatga caaagtggtt gctgaaatgc gcggggtgat
     6121 tgacgaacac ggctattcac cgttctggca aagcctcggt aaacgcttct tttcgatgga
     6181 ttttagccgc gccgattttc tctgcggcac cgggcaaaag gcatttattg cagaactgat
     6241 gccgaaacat ccgatctata cccacttttt atcccaggaa gcccaggacg tcatcggtca
     6301 ggtacatccg caaaccgcgc ctgcccgcgc ggtgctggag aaagaaggtt ttcgttaccg
     6361 caactatatc gacatcttcg acggcgggcc aacgcttgag tgtgacatcg accgcgtgcg
     6421 cgccatccgt aaaagtcggc tggtggaagt ggcagcaggc tggcttgccg ccgggcgtga
     6481 aacgggtcag gcgctgagtg cgctggagga tctcgacggt ttgctgttta ccggtagcgc
     6541 caataccggc taccagctgc atcgccagct ctccggtcag ccggagaaaa ttctcgccct
     6601 tgagatgggc ggtaataatc cgctaattat cgatgaggtg gcggatatcg acgcggctgt
     6661 ccatctgacc attcagtcgg cgtttgtcac agccgggcaa cgctgcacct gcgcccgccg
     6721 tttattgctc aaaagcggag cgcagggcga tgcgtttctt gctcgtctgg ttgccgtcag
     6781 ccagcgatta acgccgggca actgggatga cgaaccgcag ccgtttattg gcgggctgat
     6841 ttctgaacag gccgcacagc aggtggttac tgcctggcag caactggaag cgatgggcgg
     6901 acgaaccctg cttgcgccgc gcttattaca agcagggaca tcgttgctga caccagggat
     6961 cattgaaatg acgggcgttg gcggtgtgcc ggatgaagag gtgttcgggc cgcttttgcg
     7021 cgtctggcgt tatgatactt ttgatgaggc gattcggatg gcgaacaaca ctcgcttcgg
     7081 actcttttgc ggtctggttt cccccgagcg ggaaaaattc gatcaactgt tgctggaggc
     7141 gcgggcgggg attgttaact ggaacaaacc gcttactggt gctgccagta ccgcgccatt
     7201 cggcggcatt ggtgcttccg gtaaccatcg ccccagcgcc tggtatgccg cagattactg
     7261 cgcatggcct atggcgagcc tggagtcgga ctcgttaaca ttgcccgcca cgcttaaccc
     7321 cgggctggat ttttccgatg aggtggtgcg atgaacgcct gggaagttaa tttcgacggg
     7381 ctggtagggc tgacgcatca ttacgcgggc ctgtcgtttg gtaatgaagc ctctacccgt
     7441 caccgttttc aggtgtctaa cccgcgactg gcggcaaaac agggcttact gaaaatgaaa
     7501 gcccttgccg atgcgggatt cccccaggcc gtgatcccgc cgcacgagcg tccgtttatt
     7561 ccggtgctgc gtcagttggg attcagtggt agcgatgagc aggtactgga aaaagttgca
     7621 cgccaggcac cgcactggct ttccagcgtc agttccgctt cgccaatgtg ggtagccaat
     7681 gcggcaacga tcgcgccatc tgccgatacg ctggatggca aagtgcatct caccgttgcc
     7741 aacctgaaca ataaatttca ccgttcgctg gaagcgcccg tcactgaatc gctgttaaaa
     7801 gcgattttta aggacgaaga gaaatttagc gtccattcgg cgttgccgca ggtagcgttg
     7861 ctcggtgatg agggggcggc aaaccacaat cgtctcggcg gtcattacgg tgaaccgggt
     7921 atgctacttt ttgtctacgg gcgagaagaa ggcaatgata cccggccttc ccgttatccg
     7981 gcgcgacaga ctcgcgaagc cagcgaggcg gtggcaaggc ttaatcaggt gaatccccaa
     8041 caggtgattt tcgcccagca aaacccggac gttatcgacc agagcgtttt tcataatgac
     8101 gtgattgccg tgagtaaccg ccaggtgctg ttttgccacc aacaggcgtt cgctcgccag
     8161 tcacagttac tggcaaacct gcgggcgcgg gtgaacggtt ttatggcgat agaagttccg
     8221 gcaactcagg tttccgtgtc ggatgcggtg tctacctatc tgtttaacag ccaactgttg
     8281 agccgcgatg atggttccat gatgttggtg ctgcctcagg agtgtcggga acacgccgga
     8341 gtatggggtt atctcaatga actccttgcc gctgacaacc cgattagcga actaaaagtc
     8401 tttgacttac gtgaaagcat ggcgaatggc ggcggcccgg cgtgcctgcg gttgcgggtg
     8461 gtattgacag aagaag
//
LOCUS       DAJTNM010000147         8391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTNM010000147 DAJTNM010000000
VERSION     DAJTNM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.147"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="OO172_003596"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="OO172_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5513618.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(964..1437)
                     /locus_tag="OO172_003597"
     CDS             complement(964..1437)
                     /locus_tag="OO172_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5513619.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1597..2610
                     /gene="intI1"
                     /locus_tag="OO172_003598"
     CDS             1597..2610
                     /gene="intI1"
                     /locus_tag="OO172_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5513620.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2813..3163
                     /locus_tag="OO172_003599"
     CDS             2813..3163
                     /locus_tag="OO172_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5513621.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3289..3849
                     /locus_tag="OO172_003600"
     CDS             3289..3849
                     /locus_tag="OO172_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5513622.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3852..6818
                     /locus_tag="OO172_003601"
     CDS             3852..6818
                     /locus_tag="OO172_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5513623.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6885..7262
                     /locus_tag="OO172_003602"
     CDS             6885..7262
                     /locus_tag="OO172_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5513624.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7463..8122)
                     /locus_tag="OO172_003603"
     CDS             complement(7463..8122)
                     /locus_tag="OO172_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5513625.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1850 a         2439 c         2284 g         1818 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc gcttccctca tgatgtttaa cgcctggcac agcggatcgc
      901 aaacctggcg cggcttttgg tacaaaaggc gtgacaggtt tgcgaatccg ttgctgccac
      961 ttgttaaccc ttttgccaga tttggtaact ataatttatg ttagaggcga agtcttgggt
     1021 aaaaactggc ctaaaattgc tggggatttc aggaaagtaa acatcacctt ccggctcgat
     1081 gtctattgta gatatatgta gtgtatctac ttgatcgatc aggcttttgt atatctcccc
     1141 accacctgaa acaatgacat gatccgttat tttctttagg ttggttaaag catctttaat
     1201 tgatggaaag atcactacgt tctcattgtc agatgtaaaa cttgaacgtg ttacgaccgc
     1261 atactttcgg ttgggtaatg ctcccattga ttcaaaagtc ttgcgtccaa ccaacagcca
     1321 ttggttatag gtaatagctt taaacaggag ctgttcacct ttggcactcc atggaatatc
     1381 agggccattc ccgataactc cattcttcga tatagctacc attagtgata gtttcacaat
     1441 tcttcctcag aggttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     1501 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     1561 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     1621 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     1681 tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgccttcat ccgtttccac
     1741 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
     1801 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
     1861 ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg
     1921 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
     1981 ctggaaggcg agcatcgttt gttcgcccag cttctgtatg gaacgggcat gcggatcagt
     2041 gagggtttgc aactgcgggt caaggatctg gatttcgatc acggcacgat catcgtgcgg
     2101 gagggcaagg gctccaagga tcgggccttg atgttacccg agagcttggc acccagcctg
     2161 cgcgagcagc tgtcgcgtgc acgggcatgg tggctgaagg accaggccga gggccgcagc
     2221 ggcgttgcgc ttcccgacgc ccttgagcgg aagtatccgc gcgccgggca ttcctggccg
     2281 tggttctggg tttttgcgca gcacacgcat tcgaccgatc cacggagcgg tgtcgtgcgt
     2341 cgccatcaca tgtatgacca gacctttcag cgcgccttca aacgtgccgt agaacaagca
     2401 ggcatcacga agcccgccac accgcacacc ctccgccact cgttcgcgac ggccttgctc
     2461 cgcagcggtt acgacattcg aaccgtgcag gatctgctcg gccattccga cgtctctacg
     2521 acgatgattt acacgcatgt gctgaaagtt ggcggtgccg gagtgcgctc accgcttgat
     2581 gcgctgccgc ccctcactag tgagaggtag ggcagcgcaa gtcaatcctg gcggattcac
     2641 tacccctgcg cgaaggccat cggtgccgca tcgaacggcc ggttgcggaa agtcctccct
     2701 gcgtccgctg atggccggca gcagcccgtc gttgcctgat ggatccaacc cctccgctgc
     2761 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga caatggaggt
     2821 ggtagccgag ggtgtggaaa cacccgactg ccttgcgtgg ttgcggcagg cgggttgcga
     2881 cacggtgcag ggtttcctgt tcgccaggcc gatgccggcg gcggccttcg tcggcttcgt
     2941 caaccaatgg aggaacacca ccatgaacgc caatgaaccg agcaccagtt gctgcgtgtg
     3001 ctgcaaggaa atcccgctcg atgccgcctt cacgccggaa ggggccgagt acgtggagca
     3061 tttctgcggg ctggagtgct atcagcgctt ccaggcgcgg gccagcactg cgaccgaaac
     3121 cagcgtcaaa ccggacgctt gtgattcgcc gccgtcaggt tgaggcatac cctaacctga
     3181 tgtcagatgc catgtgtaaa ttgcgtcagg ataggattga attttgaatt tattgacata
     3241 tctcgttgaa ggtcatagag tcttccctga cattttgcag ggaattccat gactggacag
     3301 cgcattgggt atatcagggt cagcaccttc gaccagaacc cggaacggca actggaaggc
     3361 gtcaaggttg atcgcgcttt tagcgacaag gcatccggca aggatgtcaa gcgtccgcaa
     3421 ctggaagcgc tgataagctt cgcccgcacc ggcgacaccg tggtggtgca tagcatggat
     3481 cgcctggcgc gcaatctcga tgatttgcgc cggatcgtgc aaacgctgac acaacgcggc
     3541 gtgcatatcg aattcgtcaa ggaacacctc agttttactg gcgaagactc tccgatggcg
     3601 aacctgatgc tctcggtgat gggcgcgttc gccgagttcg agcgcgccct gatccgcgag
     3661 cgtcagcgcg agggtattgc gctcgccaag caacgcgggg cttaccgtgg caggaagaaa
     3721 tccctgtcgt ctgagcgtat tgccgaactg cgccaacgtg tcgaggctgg cgagcaaaag
     3781 accaagcttg ctcgtgaatt cggaatcagt cgcgaaaccc tgtatcaata cttgagaacg
     3841 gatcagtaaa tatgccacgt cgttccatcc tgtccgccgc cgagcgggaa agcctgctgg
     3901 cgttgccgga ctccaaggac gacctgatcc gacattacac attcaacgat accgacctct
     3961 cgatcatccg acagcggcgc gggccagcca atcggctggg cttcgcggtg cagctctgtt
     4021 acctgcgctt tcccggcgtc atcctgggcg tcgatgaact accgttcccg cccttgttga
     4081 agctggtcgc cgaccagctc aaggtcggcg tcgaaagctg gaacgagtac ggccagcggg
     4141 agcagacccg gcgcgagcac ctgagcgagc tgcaaaccgt gttcggtttc cggcccttca
     4201 ccatgagcca ttaccggcag gccgtccaga tgctgaccga gctggcgatg caaaccgaca
     4261 aaggcatcgt gctggccagc gccttgatcg ggcacctgcg gcggcagtcg gtcattctgc
     4321 ccgccctcaa cgccgtcgag cgggcgagtg ccgaggcgat cacccgtgct aaccggcgca
     4381 tctacgacgc cttggccgaa ccactggcgg acgcgcatcg ccgccgcctc gacgatctgc
     4441 tcaagcgccg ggacaacggc aagacgacct ggttggcttg gttgcgccag tctccggcca
     4501 agccaaattc gcggcatatg ctggaacaca tcgaacgcct caaggcatgg caggcactcg
     4561 atctgcctac cggcatcgag cggctggttc accagaaccg cctgctcaag attgcccgcg
     4621 agggcggcca gatgacaccc gccgacctgg ccaaattcga gccgcaacgg cgctacgcca
     4681 ctctcgtggc gctggccacc gagggcatgg ccaccgtcac cgacgaaatc atcgacctgc
     4741 acgaccgcat cctgggtaag ctgtttaacg ctgccaagaa taagcatcag cagcagttcc
     4801 aggcgtcagg caaggccatc aacgccaagg tacgtctgta cgggcgcatc ggtcaggcgc
     4861 tgatcgacgc caagcaatca ggccgcgatg cgtttgccgc catcgaggcc gtcatgtcct
     4921 gggattcctt tgccgagagc gtcaccgagg cgcagaagct cgcgcaaccc gatgacttcg
     4981 atttcctgca tcgcatcggc gagagctacg ccaccctgcg ccgctatgca ccggaattcc
     5041 ttgccgtgct caagctgcgg gccgcgcccg ccgccaaaaa cgtgcttgat gccattgagg
     5101 tgctgcgcgg catgaacacc gacaacgccc gcaagctgcc agccgatgca ccgaccggct
     5161 tcatcaagcc gcgctggcag aaactggtga tgaccgacgc cggcatcgac cggcgctact
     5221 acgaactgtg cgcgctgtcc gagttgaaga actccctgcg ctcgggcgac atctgggtgc
     5281 agggttcacg ccagttcaag gacttcgagg actacctggt accgcccgag aagttcacca
     5341 gcctcaagca gtccagcgaa ttgccgctgg ccgtggccac cgactgcgaa caatatctgc
     5401 atgagcggct gacgctgctg gaagcacaac ttgccaccgt caaccgcatg gcggcagcca
     5461 acgacctgcc ggatgccatc atcaccgagt cgggcttgaa gatcacgccg ctggatgcgg
     5521 cggtgcccga caccgcgcag gcgctgatag accagacagc catggtcctg ccgcacgtca
     5581 agatcaccga actgctgctc gaagtcgatg agtggacggg cttcacccgg cacttcacgc
     5641 acttgaaatc gggcgatctg gccaaggaca agaacctgtt gttgaccacg atcctggccg
     5701 acgcgatcaa cctgggcctg accaagatgg ccgagtcctg ccccggcacg acctacgcga
     5761 agctcgcttg gctgcaagcc tggcataccc gcgacgaaac gtactcgaca gcgttggctg
     5821 aactggtcaa cgctcagttt cggcatccct ttgccgggca ctggggcgat ggcaccacat
     5881 catcatcgga cggacagaat ttccgaaccg ctagcaaggc aaagagcacg gggcacatca
     5941 acccaaaata tggcagcagc ccaggacgga ctttctacac ccacatctcc gaccaatacg
     6001 cgccattcca caccaaggtg gtcaatgtcg gcctgcgcga ctcaacctac gtgctcgacg
     6061 gcctgctgta ccacgaatcc gacctgcgga tcgaggagca ctacaccgac acggcgggct
     6121 tcaccgatca cgtcttcgcc ctgatgcacc tcttgggctt ccgcttcgcg ccgcgcatcc
     6181 gcgacctggg cgacaccaag ctctacatcc cgaagggcga tgccgcctat gacgcgctca
     6241 agccgatgat cggcggcacg ctcaacatca agcacgtccg cgcccattgg gacgaaatcc
     6301 tgcggctggc cacctcgatc aagcagggca cggtgacggc ctcgctgatg ctcaggaaac
     6361 tcggcagcta cccgcgccag aacggcttgg ccgtcgcgct gcgcgagttg ggccgcatcg
     6421 agcgcacgct gttcatcctc gactggctgc aaagcgtcga gctacgccgc cgcgtgcatg
     6481 ccgggctgaa caagggcgag gcgcgcaatg cgctggcccg tgccgtgttc ttcaaccgcc
     6541 ttggtgaaat ccgtgaccgc agtttcgagc agcagcgcta ccgggccagc ggcctcaacc
     6601 tggtgacggc ggccatcgtg ctgtggaaca cggtctacct ggagcgtgcg gcgcatgcgt
     6661 tgcgcggcaa tggtcatgcc gtcgatgact cgctattgca gtacctgtcg ccactcggct
     6721 gggagcacat caacctgacc ggtgattacc tatggcgcag cagcgccaag atcggcgcgg
     6781 ggaagttcag gccgctacgg cctctgcaac cggcttagcg tgctttattt tccgttttct
     6841 gagacgaccc ctattatggc gaaggtatca ttcaggaagc gttgatgaag gattatctct
     6901 gtaaaaagtt attcaaccgg ctttccggta ccctggtgat cagagcgcgc tgcggcaata
     6961 acattactgg cctggcatgt tgcaatattc tttatccctc gccccgatac agcggtcagc
     7021 tgcatattaa agagctgtat gtttctcagt gcgatcgaaa taagggcacg ggtaaagcga
     7081 taatgcgctt tatagcccgg cttgcgcttg aacaggaatg ccttagcctt agctggaacg
     7141 ctgaaaaatc caaccccggc gctaaccgtt tttatcaggc tctgggaggc agaataaatg
     7201 atcatatcgt caattattac ctccacgggg agagcctgag caaactggcc tcaggcattt
     7261 gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca
     7321 taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat
     7381 ttctgccatt catccgctta ttatcactta ttcaggcgta gcaaccaggc gtttaagggc
     7441 accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta
     7501 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     7561 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     7621 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     7681 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     7741 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     7801 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     7861 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     7921 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     7981 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     8041 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     8101 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     8161 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     8221 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     8281 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     8341 cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg a
//
LOCUS       DAJTNM010000148         8350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTNM010000148 DAJTNM010000000
VERSION     DAJTNM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.148"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..157
                     /locus_tag="OO172_003604"
     CDS             <1..157
                     /locus_tag="OO172_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513626.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO172_003605"
     CDS             complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO172_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5513627.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO172_003606"
     CDS             complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO172_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5513628.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            873..1292
                     /locus_tag="OO172_003607"
     CDS             873..1292
                     /locus_tag="OO172_003607"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5513629.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1279..6144
                     /gene="traI"
                     /locus_tag="OO172_003608"
     CDS             1279..6144
                     /gene="traI"
                     /locus_tag="OO172_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR5513630.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISSEQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQVETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPR
                     LEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEA
                     REQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWE
                     NNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRV
                     GTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQAYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPT
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQA
                     VREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVR
                     DLQKEKTPGGD"
     gene            6164..6910
                     /gene="traX"
                     /locus_tag="OO172_003609"
     CDS             6164..6910
                     /gene="traX"
                     /locus_tag="OO172_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5513631.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6965..7525
                     /locus_tag="OO172_003610"
     CDS             6965..7525
                     /locus_tag="OO172_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5513632.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPWLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7656..7868
                     /locus_tag="OO172_003611"
     CDS             7656..7868
                     /locus_tag="OO172_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5513633.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8112..>8350
                     /locus_tag="OO172_003612"
     CDS             8112..>8350
                     /locus_tag="OO172_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5513634.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1917 a         2095 c         2673 g         1665 t
ORIGIN      
        1 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc aggaaaatca
       61 tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga
      121 acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc tccagtcttc
      181 agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa taatcagccc
      241 ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc gtccctgacg
      301 ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac
      361 atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca tctggctttt
      421 ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattt tcccctggtt
      481 caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt
      541 ggtatcgagc ataaacttca gcatcagaat gactcccttt cctgcatgcc gggctgttcc
      601 ctgttatcca taaaatcggt gctgacgctg tgtccgtcga accattcgtc ccatgtctct
      661 ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg ttttacgttt
      721 tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct gagaaatacg
      781 gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt
      841 gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg
      901 ccggaagtgc cggtaattat tataccgaca aggataatta ctatgtgctg ggcagcatgg
      961 gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt gtcgataagg
     1021 atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta agccgcatgc
     1081 aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct cccaaaagta
     1141 tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat aaccaggccg
     1201 tggattttgc tgtccgtcat cacccgtgtg gaggcactgg cctccacacg ggtgatgacg
     1261 gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt taaccacgac
     1321 accagtcgcg atcaggaacc acagttacac acgcatgcgg tggtgactaa tgttacgcag
     1381 tataacggcg aatggaagac actgagcagt gacaaagtgg ggaaaacggg gttcattgag
     1441 aatgtgtacg ctaatcagat tgcctttggc aggctctacc gggaaaaact gaaagagcag
     1501 gttgaggcgc tgggctatga aactgaagtg gtcggtaagc acggtatgtg ggaaatgccg
     1561 ggtgtaccgg tggaggcctt ttccggacgc tcacagacta tccgggaggc cgtcggggaa
     1621 gacgcctcgc tgaaatcccg ggatgtggcg gcgctggata cgcgtaaatc caaacagcac
     1681 gtcgatcctg aagtcagaat ggccgagtgg atgcagacgc tgaaggaaac cgggttcgac
     1741 atccgggcat atcgtgacgc agcggaacaa cgggcatata cccgcacgca gacacccgga
     1801 cccgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggccat tgccggatta
     1861 agtgaacgca aagtgcagtt tatgtatacg gacctgctgg ccaggacggt cggcatactg
     1921 ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc catcagcagt
     1981 gagcagctta tccccctcga tcgtgagaag gggctgttca cgtccgggat tcatatgctc
     2041 gatgagctgt cagtccgggc actcagtcgt gacatcatga aacagaaccg ggtgaccgta
     2101 catccggaga aaagtgtccc ccggacggcc ggttacagcg atgccgtcag tgttctggca
     2161 caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg gcagcgtgag
     2221 cgggtggctg aactggtcat gatggcccgg gagcaggggc gggaggtgca gattatcgct
     2281 gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg tgaactgata
     2341 accggacgtc gtcagttgct ggaaggcatg gccttcacgc cgggcagtac cgttatcgtc
     2401 gaccagggcg aaaaactctc cctgaaagag acgttaaccc tgctggatgg tgccgcacgt
     2461 cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg cagtgcactg
     2521 atggccatga aggatgccgg ggtgaacaca tatcgctggc aggggggaga acagcgaccg
     2581 gccaccatca tcagtgaacc ggaccgtaat gtccgctatg cccggctggc cggagatttt
     2641 gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggagt acgggaacag
     2701 gccatactga cacaggccat tcgcagtgag ctgaaaacac agggcgtgct cggacacccg
     2761 gaggtaacca tgaccgccct gtcaccggtc tggctggaca gccggagccg ttatctgcgg
     2821 gatatgtacc gcccgggaat ggtgatggag cagtggaacc cggagacacg cagtcatgac
     2881 cgctatgtga cagaacgtgt gacggcgcag agtcacagcc tgaccctgcg gaatgcgcag
     2941 gtcgaaacgc aagtggtgcg tatttcttcc ctggacagca gctggtcgct gttccggccg
     3001 gaaaaaatgc cggtggcaga cggtgagcgt ctgagggtga cagggaaaat tcccggactc
     3061 cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc gatgacggtt
     3121 gttgtgccgg gacgggctga accggccacc ctgcctgtga gcgattcacc gttcacggca
     3181 ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagcga cagtgcgacg
     3241 gtttttgcct ccgtcacaca gatggcaatg gacaatgcca ccctgaatgg tctggcccgc
     3301 agtggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc ggaaaaactt
     3361 gcccgccatc cctcctttac ggtggtttct gagcagataa aggcgcgtgc cggtgagaca
     3421 ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc acagcaggcc
     3481 attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat ggtggacctg
     3541 ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttgctgacct gggaggggaa
     3601 atcaatgcgc agataaaacg gggtgattta ctgtatgtgg atgtggcaaa aggctatggc
     3661 acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct tcgccatatt
     3721 ctcgaaggta aggaggcggt cacgccgctg atggagagag tacctggcga actcatggag
     3781 aaactgacat caggacagcg cgccgccacc cgtatgatac tggaaacgtc tgaccgtttc
     3841 acggtggtgc agggctatgc cggtgtgggt aagaccacac agttccgggc ggtgatgtca
     3901 gccgtgaaca tgctgccgga gagtgaacgt ccccgtgtcg tggggctggg gcccacacac
     3961 cgtgcggtcg gggagatgcg cagcgccggc gtggatgcac agacactggc gtcctttctg
     4021 catgacacgc agcagcgcag cggagaaacg ccggatttca gcaacacgct gttccttctc
     4081 gatgagagct ctatggtggg caataccgac atggcacggg catacgccct gattgcggcc
     4141 ggtggcggtc gggctgtggc cagcggtgac acggaccagc tgcaggccat cgcgcccggt
     4201 cagcctttcc gtctccagca gacgcgcagt gctgccgatg tggtcatcat gaaggagatt
     4261 gtgcgtcaga cgccggaact gcgggaggcg gtatacagcc tgattaaccg ggatgtggaa
     4321 agggcgctgt ccgggcttga gcgtgtgaaa ccgtctcagg tgccacgtct ggagggcgca
     4381 tgggcaccgg agcactccgt gacggagttc agtcacagcc aggaagcgaa actggcagaa
     4441 gcgcagcaga aggcgatgct gaaaggcgag gcttttccgg atgtccccat gacactgtat
     4501 gaagccattg tccgcgacta taccggcagg acaccggaag cacgggagca gacgctgatt
     4561 gtcacgcacc tgaatgagga ccggcgcgta ctgaacagca tgattcatga tgcacgggaa
     4621 aaggccggag aactggggaa ggtgcaggtc atggtgcctg tcctgaacac agcgaatata
     4681 cgcgacgggg agctgcgtcg tctctccacc tgggagaata acccggatgc ccttgccctg
     4741 gtggataatg tgtatcaccg gattgccggt atcagcaagg atgacgggct gataaccctg
     4801 caggatgcgg aaggtaacac gcggctgatt tcgccccggg aggcggtggc tgaaggcgtc
     4861 acgctgtaca ccccggacac catccgggtg gggaccggtg acaggatacg cttcacgaag
     4921 agtgaccggg agcgcggtta tgtggccaac agcgtctgga cggtgacagc ggtttccggt
     4981 gacagtgtca cactttctga cggtcagcag acccgtgtga ttcgccccgg tcaggagcgg
     5041 gcagagcaac atattgacct ggcctatgcc atcaccgccc acggtgcaca gggggcgagt
     5101 gaaacctttg ccatcgcgct tgaaggcacg gaaggtaacc ggaaactgat ggccggcttt
     5161 gagtcagcct acgtggccct gtcgcgtatg aagcagcatg tgcaggcgta caccgataac
     5221 cgtcagggct ggacggatgc catcaacaat gccgtacaga aaggaactgc ccacgatgtg
     5281 tttgagccga aaccggaccg ggaggtcatg aatgcagagc ggctgttcag tacggcgcgg
     5341 gagctgcggg acgtggcggc aggccgtgcc gttctccgtc aggcggggct ggccggggga
     5401 gacagtccta cacggtttat tgctccggga cgtaaatatc cgcaaccgta tgtggcactg
     5461 ccggcgtttg accgtaacgg caagtccgcc ggtatctggc tgaacccgct gaccacggat
     5521 gacggaaacg ggctgcgggg attcagcggt gaaggtcggg taaaaggcag cggggatgcg
     5581 cagttcgtgg ccctgcaggg cagccgtaac ggagaaagcc tgctggctga taatatgcag
     5641 gatggcgtcc ggattgcccg tgataatcct gacagtggtg tggtggtaag aatcgccggt
     5701 gaaggccgtc cgtggaatcc cggtgccatc accggtggcc gcgtgtgggg ggatatcccg
     5761 gacaacagcg tccagccggg agccggaaat ggcgaaccgg tcacggcaga ggtactggca
     5821 cagcggcagg ctgaagaggc catccgccgt gaaacggaac gtcgcgcaga tgaaattgtc
     5881 cgtaaaatgg cagagaacaa acctgacctg ccggacggca aaacagagca ggctgtcagg
     5941 gaaattgccg gtcaggagcg tgaccgggct gccataactg aacgggaagc cgcgctgccg
     6001 gagagtgtac tgcgtgaacc acaacgggtg cgggaagcgg tccgggaagt tgcccgggaa
     6061 aatttgctgc aggagcgtct gcagcagatg gagcgggata tggttcgtga cctgcagaaa
     6121 gagaaaaccc cgggcggaga ctgatacagg aagacaaact ctgatgacaa cggataacac
     6181 gaacacgaca cgtaacgatt cactggctgc acggaccgat acctggttgc agtcatttct
     6241 ggtctggtca cccggacagc gggatatcat caaaacggtg gcactggtgc tgatggtact
     6301 ggaccacatc aatctgatat tccagctgaa gcaggaatgg atgtttctgg ccgggcgagg
     6361 agcctttccg ctttttgccc tggtgtgggg gctgaatctg tcccgtcatg cgcatatccg
     6421 gcaaccggcc attaaccggc tgtggggatg gggaattatt gcccagttcg catattatct
     6481 ggcaggcttt ccttggtatg aggggaatat cctgtttgcc tttgcagtgg cggcacaggt
     6541 gctgacgtgg tgtgaaacgc gcagtgggtg gcgtacagca gcggccatac tcctgatggc
     6601 cctgtggggg cctttgtccg gcaccagtta cggtattgcc gggttgctga tgctggcagt
     6661 cagcaaccgg ctgtaccggg cggaagacag agcggaacgt ctggcgctgg tcgcctgcct
     6721 gctggctgtt attccggcgc ttaaccttgc caccagtgat gcagcggcgg tggccggtct
     6781 tgtgatgacg gtgctgaccg ttggtctggt gttgtgtgcc ggaaaatcat taccccgttt
     6841 ctggcccgga gactttttcc cgacgttcta tgcctgtcat ctggctgtgc tgggcgttct
     6901 ggcgctgtga cgggtgtggt atctttggcc gcaagaggat gattcgtcag aggcagaaca
     6961 cagcatgata gagcagaagc gaccggtact gacactgaag cggaagacag agggaacagc
     7021 gcctgtccgc agccggaaaa ccatcatcaa tgtcaccacg ccaccaaagt ggaaggtgaa
     7081 aaagcagaag ctggccgaga aagcggcccg tgaagcagag ctggcggcaa aaaaagcgca
     7141 ggccagacag gcgctgtcca tttatctgaa cctgcccacg ctggatgagg ctgtgaatac
     7201 cctgaagccc tggtggccgg gattatttga cggtgacacg ccctggcttc tggcctgcgg
     7261 tatccgggac gtgttactgg aagacgtggc gcagcggaat atcccgctct cgcataaaaa
     7321 actgcgcagg gcgctgaagg ccatcacccg ttcagaaagc tatctgtgtg ccatgaaagc
     7381 cggtgcctgc cggtatgaca cggaagggta tgtgacggag catatttctc aggaggagga
     7441 agcgtatgcg ggagcgcgtc tggcgaaaat tcgtcgccag aaccggataa aggcagaact
     7501 tcaggccgtg cttgatgaga aataaaaaaa gcctccccca gagagaggag gcagggaaat
     7561 aaggcttaaa aggaaataac ttccgcttcc gaaaggaagc cagtccactg aattatttat
     7621 cacaataata ctgacaacta aagggggaca agaaaatgaa tggattcaga aacagttcac
     7681 ggaacggcca ggtctggcgt taccagcgtg ccggcgggcg ggccgttatt ctggaagtca
     7741 gtggacgctg gatggaagcg gcagaagcat ggcgacgggc tgcctgtgtt gcgcctagga
     7801 cagactggca gcagtttgcc cggaaaaggg ctgaacactg ccaccggcgc tgccggggaa
     7861 gggtgtaacg aaaaaagcag ccttcattac tctaggagtc agaaggctga aaggagttta
     7921 tgagatttgc ttcttaatat ccggtcgggc agtatgaccg gacagttctg agtagcacag
     7981 ggtcttttcc gcattaagtc gattttttat attttgtgat taatttcaca aaataaggtg
     8041 ttattcaatg tgtgctgcac tattcagggg ggctgaaata caggtggcat ttatctgata
     8101 tggagaataa catgaggaaa tatattccac tggtattatt tatcttttca tggccggtat
     8161 tgtctgcaga tattcatgga cgggttgttc gtgtactgga cggtgatacc atcgaggtca
     8221 tggactcact gaaggcggtg cggatcaggc tggtaaatat tgatgcaccg gaaaagaaac
     8281 aggattatgg ccggtggtca acagacatga tgaaatcact ggtcgcagga aaaacggtta
     8341 ccgtcacata
//
LOCUS       DAJTNM010000149         8255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTNM010000149 DAJTNM010000000
VERSION     DAJTNM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.149"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..283)
                     /locus_tag="OO172_003613"
     CDS             complement(<1..283)
                     /locus_tag="OO172_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5513635.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVY"
     gene            complement(297..956)
                     /gene="pgmB"
                     /locus_tag="OO172_003614"
     CDS             complement(297..956)
                     /gene="pgmB"
                     /locus_tag="OO172_003614"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5513636.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(953..3220)
                     /gene="ycjT"
                     /locus_tag="OO172_003615"
     CDS             complement(953..3220)
                     /gene="ycjT"
                     /locus_tag="OO172_003615"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5513637.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     ARGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(3217..3823)
                     /locus_tag="OO172_003616"
                     /pseudo
     CDS             complement(3217..3823)
                     /locus_tag="OO172_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(3871..4617)
                     /locus_tag="OO172_003617"
                     /pseudo
     CDS             complement(3871..4617)
                     /locus_tag="OO172_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
     gene            complement(4648..5490)
                     /locus_tag="OO172_003618"
     CDS             complement(4648..5490)
                     /locus_tag="OO172_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5513638.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVTWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(5477..6358)
                     /locus_tag="OO172_003619"
     CDS             complement(5477..6358)
                     /locus_tag="OO172_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5513639.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLLAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(6379..7662)
                     /locus_tag="OO172_003620"
                     /pseudo
     CDS             complement(6379..7662)
                     /locus_tag="OO172_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(7676..>8255)
                     /gene="ycjM"
                     /locus_tag="OO172_003621"
     CDS             complement(7676..>8255)
                     /gene="ycjM"
                     /locus_tag="OO172_003621"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5513640.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMDALSRRESSDEERCARFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         1984 a         2292 c         2061 g         1918 t
ORIGIN      
        1 cgtaaaccgt catatgcgga tacaacgcgt agttctggaa caccatcgcg atattgcgtg
       61 ctttggctgg aacgtcattc attcgtttgc cgtcgatcaa cagatcgccg ccgctgatct
      121 cctcaagccc ggcaatcatg cgcagggtgg tcgatttacc gcagcccgac gggccgacaa
      181 atacgatgaa ctctttatcg gcaatttcca ggttgaagtc cttcaccaca tgcacctggt
      241 tatcgtagat tttttgaata tgttgtaacg aaagctgagc catgttgatt cctttgctat
      301 acgttttgcc agaaggccga taaccgcgac caggtcaggg agtccgttga gggcaacagt
      361 aattgcgccc cggttaagcc cgcaccgatc cccaccgagc gcataccgct ggcgttaatg
      421 gcgtcaatgc ccgcctgcgc atcttcaatg ccgatacatg cctgcggcgg cacgcccagc
      481 ccggcacagg cagcgagaaa gatttccgga tccggtttcg agtttttaag ttgggaagca
      541 tccgcgcaga aggtgaaaaa ctcgcgcagc tccagcgccg ctaaaatcgt cggcgcattc
      601 agggagacgg aagccagccc taccggaatc tgctgtgcac ggagctctgc cagcaaattg
      661 cgaataccgg gcagcacagc attaaccgtc agctcgcgca gtgagtggac gtaaagcaga
      721 tttttgcgat acgccagttg cgcccgctcc tgcgggttaa agtcgccctc tttgcccccg
      781 tgttgcagaa tgcgccgcag agactcatcg cggctgatcc cttttagaga ttcgttaaac
      841 tgcgcatcaa tactgatgcc aatttcagcg gcaatctgct gccacgcctg gaaatgcaga
      901 tgcgcggtat cggtgattac accatccaga tcgaaaatta ccccttgcag tttcattcat
      961 cctcctgatg cgtggtagcg gtcccattga agggcaaaat aaaatcaccc aaacagaaaa
     1021 cagattcttc agccacggat ataagctgac cgttcaaacg cagtgaaacg ggcgcagaag
     1081 ttcgaatcgc aatacgctgc gcgtcgagag tgacctgtaa ttcgcagccc tgccagaaca
     1141 gagggaaaga caactgttgc cactgctcag gtaacgccgg atttagatgc aattcaccgt
     1201 cacgcacgct caccccggca aaaccctgaa tcgcccccag ccagatagcg ccagttgcgg
     1261 cagcatggat accatcatca caactatgcg gatcagcacc aagatcgatt tcagtcccct
     1321 cgcgccagaa ctgataactt tgggtcagca ggccacagcg tgcagcaaca atgccgtgga
     1381 ttgctttact taatgacgag tcgtgaatag tgcgcggttc ataaaattgc agattggcaa
     1441 gacacgatgc cgatgagaac tgctctggca gcatgtaatt gagcatcacc acatcagctt
     1501 gtttgaggat ctgcatctcg ttcacttctg cgcgtgaata atccagcaga atggtttgct
     1561 tccccgccgc cgctttgtat ttcgccagat taatcgccgg cttagtcata aacgaatcat
     1621 cctgcggcaa aacaccgtcg ggctgaattt ctggcagccg cagttcttta aggaacattt
     1681 cggcacgatg aataaacgcg tcgtcactac agccaaactg gcgggcaata ctcagcgcct
     1741 gctggacgtt gtaatacacc atatagctgg tgaaggcgtt attattgaca tgttcggtat
     1801 attcgtctgg cccaataaca tcatgaattt ccagacggtc gttaaccctc accgcgcggc
     1861 taatccagaa ctttgcagtt tccagaagta gcgccatgcc ttcatgcgca atgaaacttt
     1921 catcccccgt ggtctgccag tattgaataa ccgcccaggc gatatcggcc accagatgat
     1981 gttccgcctg cgccgaggcc actttttgcc gcagcccggt gcgaatgtta atggcggcaa
     2041 attccggcgt ctcttcttcg ccgctgcgcg cgctttccca cggaaatagc gcgccctgcc
     2101 agccgttgcg tcgcgctttc tcctgcgcgc ctggcaagtt gtgccagcga taacgcagta
     2161 aacttcgggc aaccgtcgga tcgctaaaca gatggaacgg cagcaaaaat acttctgtat
     2221 cccagaaaac gtggcctttg tagccttcgc cggttaagcc ttttgccgca atactgctgc
     2281 gctcgccgtg agccggcgtc atgatgcgca gatgataaag cgcataatct aacgcttgct
     2341 gatcgtgcgc atcgccgcca tttaccgtga tacgacgttt ctgccaccat tgacgccagt
     2401 tttctgttga tgctgcaaga tgttggtcgt aactctgttg cgcgcacatt tcaagctggc
     2461 gaagcgacac gctgccccac tcgtctaaag cagcttgcct gtcatcccgc cagtcgatcc
     2521 acaccagttt ttgcaacgtc actgtctcgc ctgcatgaag ctgcgcactg gtatgttgca
     2581 gtaaacggcg ctctttggcg gtatagcatt gctgcacatc accgctcacc tggcagcaac
     2641 aactgatggc cacatcactg cgcccatcct gggtggtgta gatcccctgc atcaaatgct
     2701 gaccaaacac ccgcacctgg gtttcgtcga gatgctggcg accgtggttg gtttgcgtgg
     2761 cgtcaatgcc tgttgaaatc agcactgacg cgtcggcgtc cagtggcata atagtgattt
     2821 ccagcgcaat gagcggcagt tggtctgccg aaacaaagcg acggctggcg atggtgtaac
     2881 ctgcgccgtt gctggtacgc cagacaacgc tacggcgtaa ttctccactg gcaaagtcaa
     2941 gttcacgctg ccaggcttcg cgggataacg agaaaacctc accatttatg gcaatctcca
     3001 tccccaagat atcaggcaga ttcaccagtt cgttgatttc accttttccc gcccgatgat
     3061 acagccccgc cagatacatc cctcgcgcct ggcgcgtgta atcttcttca tggctggcgc
     3121 gaagcccaag atagccgttc ccctgcgcca tcagcgatgc atacttgttc agggtatgct
     3181 ggctgaaatg gggttctgat aacgttactg gcctggtcat aattccacac gcgtccctgt
     3241 ttctgcggat tgatataacg ccgccaccag ttgctggatg atgtacccct gctcggcgtc
     3301 ggctatcatc acgggcttgc cctgtacgtg attgataaag gcttccatgc tgcgcaaatg
     3361 gcggttgtcg tctgccattt cccgttgcat cagcgtcatt aattcaccgt tgttatcggt
     3421 gtagatatgt gctggaaaca gcgtcgcacc ggctttatca ccacaaaagc tgacgttcat
     3481 aatcaactgt tcgcggatgt tgagtgcaaa tgacgtttcc agccacagaa tgccgccgtt
     3541 atgaaattca atggtgccaa acagcgaatc ttcgacgctg taagttgccg gatcccactc
     3601 gccaaattga ccacagctct tttgcgtgcc gatcttttga aaactatgcg cattcacgct
     3661 tttcaccgcc ggaaagccca gcacatacat cgcagcatcc agcatatgaa tgccgatgtc
     3721 gatccagttc tttattggta aagacacccc agccgggaac gccgcagcga cgcagggcgc
     3781 gggcggtggt aacgtaaatc tctcccaaaa cgccgttggt cacctgttca cgcagctgtt
     3841 gcgtatcgag cgcaaacgat ggtgaaagtc gtaggccagg ccgcgcagcg ccgattgcag
     3901 cgcgtcggcg taaccgctgg tttcgataat cacatcggcc ccctgcttgc cggtcagcgt
     3961 tttgatctct ttgcctacgt cagtgccaat ggggttaagg cagaaatcag caccgtggcg
     4021 acgggcaata tcacagcgat gggcgatagg atcgacgcca attaccaccg atgcgccagc
     4081 gcgtttcgcc agttggatgg caatttgacc gatcgcgcca agccctacca ccacgacaaa
     4141 atcccctacg cgtacgttgg catcgcgcac gccgctcatg gcaaactgcg ccgggtcgta
     4201 acagacggcg tttttccagg agctgccttg cggcattttg cgcagcttgt agttattcac
     4261 tgcgttaatg atgaccgtct cggagagcgg gccgtagccg catacgctgt cgcccaccgc
     4321 gtagtctgta acgtcgctgc cgcactcgat aatgtcgcca accaccatgt tgccaagctg
     4381 gaatttgcca aactcaatgc cgcgcggcgc atctgccgga cgcggggtga acatctgcca
     4441 ctcgccgtta aagtcttcat cgataaacgg gctggcggcg cggaagtcga ccacttccgt
     4501 tccgtgtttc ggtgcgccga aacgcacgcg gatcttcact tcattggcta aaatcgcccg
     4561 atcttcatac tcaaccagcg cagcaacacg cggtgctgtg gctactaact ttttcatgta
     4621 aaactccttg taaagaaact ggccgcttta gcccttcacg ccaccggcgg tcaaaccact
     4681 cttaataaaa cgttccgaaa gcgcatacat aatcaccacc ggcaatgcgg tcaccagcga
     4741 ggccgccatc atccgtcccc agatgtaatc aggcgtactg aacagcgcgt tcaggcccac
     4801 tggtaaggtg aaattgctgg cgctggagag gaaaatcgac gcaaacagat agtcgttcca
     4861 cgtcaccata aagcagtaga cgaacaccga tatcagcccg gacatcgcca ggggcacggt
     4921 gatacggaag atgatttgca gacggttgag gccatccatc atcgccgctt cttcgatctc
     4981 atcagggatg gtgtcgaagt aacttttcag cataaacacg gcggtgggca gggtttgcgt
     5041 caccatggta atgatcagcg ccatctcggt gtcgtaaata ccaagcgcgg tgatgatttt
     5101 gaacagaggc accaccagca aaatgccaga gaacatatac accgtgtaaa agctggcgtt
     5161 gatggtcatc cggcctttaa agcgcaggcg ggaaagcgcg taagctccga gaatgccgag
     5221 aaataccgcc accacggagg aaaccaccga caccaccatg ctgttacgga agtagtcgac
     5281 aaacgggaaa atcatcgggt taaaaatgtc gacgtaatgc tccagcgtcc actgttgcgg
     5341 cagcagcgta ggatgcagcg atatcgcctc tttcgcgctt ttgaacgagg tcatcagcat
     5401 cacaaaaaac ggaaacagcg tgatgatgag aaacagcgcc agcccgcagt aaaaaccgat
     5461 acgactgagc gtgcgtttat ttgttgccat tgaggttcac ccgtttcctg gtcagcagaa
     5521 tgacagcgaa aataatgatg aagagcacca ccgagatcgc cgccgctttc cctaagtcat
     5581 taaaggcaaa ggcggttttg tagagataga caccaagaat atcgaccttg gttgtcagca
     5641 gataaacatc agcgaacatg tagaacatcc agatggtgcg cagggtcacc accgtcgcca
     5701 agaccggcat aattgcgggc agcgtgacga tgcgaaaccg ctgccaggca ttagcgccat
     5761 ccatctctgc cgcttcgtac agcgatttgt cgatggtctg caaaatcgcc agaaacgaga
     5821 taaaggcata cgggaagtag cgccagatgg cgagcagcac caccagcaca aagctactgc
     5881 ccggattatc aaaccacagc ggtgcctgct catagagatg cagaagatcg acgccgaggt
     5941 agttcacaat gccgtagccg ttgttgaaca tgtatttcca ggcaaacacc agcgaaatgg
     6001 acggcgttac gtaggagagg atcaccagcg aacgcgcggt tttgcgcagg cggaactcgc
     6061 ggttgaaaaa catcgccacg gccagcccga gcacggtgct gcccgccacc accagcgccg
     6121 tataccagac cgtcatccac agcgagtgcc agaagccggg atcggagaga atgcgcacat
     6181 agttactcac cccaacaaac gttgactcga tgttgggatt gagcggcaga cgtaaaaaac
     6241 tgatttcgat attcgacacc atcggccacg ccaccagacc gcccagcagt aataagctgg
     6301 gcgcgagaag cagcagcgca aacggcatat cggaacgacc tgaaaaaagc ctgttcattg
     6361 cggattcctg tcctgggttt agtgctgttc gatcagttca tccagctttt tctggctcgc
     6421 ttgcagcgtg ccagggagat cggctttacc cacggtgacg ttatgcacca tggagctcac
     6481 cacaccagaa cccgtcacat cgcccatgcg ggtaaagttt ttatccccta ctgcgccaaa
     6541 gacctgaata tttggcagtt caccgattag ctgattcggt agttcaccca gcgccttaat
     6601 aacgtcgttg tctttccaag tggcggtagt gacaaccgct ttattcaccg gcagcgcagc
     6661 acctggcgac atcatcaccc agtcggcaat gttgtccgcc tgctccataa aggtgacaaa
     6721 tttttctgct gcttcggtct tttgtccggc ggtaatggtc agcgaggtca acatgccgta
     6781 gaccgcagag tttttctcgg ttggcaccac gaaaccgacg tttttcgggt cgccttcttt
     6841 aatcacagcc ggaaggatat aggtggagta aatcgccatc ggcgcggtgc cgttcataaa
     6901 ggcgtctttc acttccatga tgtcgttaga acccggcatg gtgttggcag caaggtcgcg
     6961 gtaataggtc agcgcctgca tcatctctgg tgtatcaagg gtgattttgc cttcggcgtt
     7021 aaagacgttg gcctagttgg ataacgcaaa ctgggagaag gattgttccg tcagcacgct
     7081 ttctgccgta ggcagcgcaa taccgtattt tttattcgcc gggtcattca gtttctgtgc
     7141 aacgtccagc agctgttgcc agtttttcgg ctcctccagc cccgcttttg ccagcacatc
     7201 tttgcgatac cagatgccgc caatccaggc gctgacagga acaccggtcc atgcgctacc
     7261 atcttcggta cgcacaatac gcagtacgcc atcgtaaaac gcgccttcac caacgttgct
     7321 gatgaccgtg gcaaccgctt tgcgatcgat aagctgctct ttatccataa ctttggcgta
     7381 gtcatggctg gtttcgatca cttccggcag cgagccgcta cgtgaaaggg tgatgacttt
     7441 ggtgttataa gcgtcttctt ccacgggcac ttgtttaacg gtgatacctg ggttttcttt
     7501 ttcaaaacgg gcaatcaatt tgctgataac tgcctgacgc tcctgctcca ccgaagaatg
     7561 cataaattcg atggatacat gggttttatt ttcttcttta cagcctgaaa tcagggcgca
     7621 ggaaaccagt gctgataaca gcacgatttt tgatttaatc atgagatgtt cctttttatt
     7681 taatccacat aacctgccaa gggcgcagcg ttatttcatt acccaatata tcaacttcac
     7741 taatcagatc ccgaccatgc agatcagtaa tatttacatg ctgaatattt ttactgacat
     7801 taaacaatcc agtcagacaa ttaccatcag cgttacttct ttgaatacgc attacggatg
     7861 aattaaccgt atcgatggta aaattattat ccggatgaaa ctcgttatgg ctgcgacgaa
     7921 gtgtaattaa acgcgacaac tcatgatata ccgcatgcct taatgtagct tcatcgttca
     7981 gttctcgtgt tatctcttca ctgtgatatt ttttacggtt aatcgcacgg ttatatccga
     8041 gtttttcgac acctgcgtaa tcattacgcg agccaagaat actttgaata tatatcgcgg
     8101 gtacaccggg gaaacttaac aaaatcgcat gggcaaggat aaacctggcg caacgttctt
     8161 catcgctact ctcgcggcgg cttaacgcat ccatataggt gacattcatc tcatagggac
     8221 tgcgcgtgcc gtcgggatta tttttccagt ttact
//
LOCUS       DAJTNM010000150         8190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTNM010000150 DAJTNM010000000
VERSION     DAJTNM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.150"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..450
                     /locus_tag="OO172_003622"
     CDS             <1..450
                     /locus_tag="OO172_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5513641.1"
                     /translation="NNASYQLNNALPNKETISSNFCERLEQWGNKSVNNGEERAIAVE
                     RIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSV
                     NKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            560..1891
                     /locus_tag="OO172_003623"
     CDS             560..1891
                     /locus_tag="OO172_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5513642.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYIPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2215..3603
                     /gene="narU"
                     /locus_tag="OO172_003624"
                     /pseudo
     CDS             2215..3603
                     /gene="narU"
                     /locus_tag="OO172_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            3685..7318
                     /gene="narZ"
                     /locus_tag="OO172_003625"
                     /pseudo
     CDS             3685..7318
                     /gene="narZ"
                     /locus_tag="OO172_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            7315..>8190
                     /gene="narH"
                     /locus_tag="OO172_003626"
     CDS             7315..>8190
                     /gene="narH"
                     /locus_tag="OO172_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5513643.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         2187 a         2029 c         1909 g         2065 t
ORIGIN      
        1 aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat ctccagcaat
       61 ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga agaaagagca
      121 attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct tgatttgtca
      181 tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt aaatctggaa
      241 aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa tatcaacctc
      301 agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga aaacatttat
      361 attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt ggttaatctg
      421 gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac tgaaatttct
      481 taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat ctctgattga
      541 gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc ccaagatgaa
      601 aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca ctacaaaact
      661 ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg atcaactaat
      721 atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc cacttgcaca
      781 aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag tgaccattga
      841 tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca cctctatcaa
      901 tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac tcgtaaagct
      961 aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa atgtaagtat
     1021 tacacatata tctatgaata ataatgctct cagaaatatt gatatagata ggctttcatc
     1081 agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag aatcttgcga
     1141 atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg caggtaataa
     1201 aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac acaatgtctt
     1261 atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa ttaaaatatt
     1321 ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt tgaaatatat
     1381 aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg acaataataa
     1441 aataacccac attgatacta ataatacatc cgatattgga actttattcc ccataataaa
     1501 acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc ctactatgca
     1561 gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc tggcaattct
     1621 ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg aattgtttca
     1681 ctatactatt aaaaaagcat atatcccata taatatacac acttacaaga cagaggaagt
     1741 tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa acaatactta
     1801 ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact ttctggacag
     1861 atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat tctataagtg
     1921 tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat ctgaagggtt
     1981 gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt tagttacatt
     2041 tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg ctttcttatc
     2101 tattctttca atcagcactt cacccaattc atccttaatt ctaataactg gctattgcgc
     2161 cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa caatatggca
     2221 ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga aaatccggcc
     2281 ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc agtcagttgt
     2341 ctacttcttg ccttctgtgt ctagatgcta tttagcgcag ttaccgttaa tctcaataaa
     2401 atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc ctccgtttct
     2461 ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg acgccaatgg
     2521 ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat tgccgtgcaa
     2581 aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg ttttgcaggt
     2641 gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc caaacaaggg
     2701 agcactcttg ggattaatgg cggattagga aacttaggtg taagtgtaat gcagctggtt
     2761 gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg cgtaccgcag
     2821 gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc gctactggcg
     2881 attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg cgcctcaatt
     2941 gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct gctttacctt
     3001 gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc aaaaacccag
     3061 ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg tgccatcgcg
     3121 cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac gttgatcaac
     3181 tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg cacaggctcc
     3241 ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc gggtctggga
     3301 agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat ttatcgggtg
     3361 aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga aacggcggca
     3421 gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc gcaggcattt
     3481 ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt tttaatcttc
     3541 tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt cagccaaaaa
     3601 taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca gatcccaatt
     3661 cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa
     3721 caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg
     3781 gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac ccacggcgtt
     3841 aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt gacctgggaa
     3901 atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga acctcgcggc
     3961 tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct gaaatacccg
     4021 ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca cagcgatccg
     4081 gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta caaacaagtg
     4141 cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct gattgccgcc
     4201 gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt ctcgccgatc
     4261 ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct gcttggcggc
     4321 acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg
     4381 ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta catcatcgct
     4441 tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac cgaagtacgc
     4501 tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc caaattgtgc
     4561 gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc aatggggcat
     4621 gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa ctactgccgc
     4681 cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg tagctacgtt
     4741 cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag caacaatccg
     4801 cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa cggttcgatt
     4861 ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc cggtacggaa
     4921 accgaattgt cgttaaccct gctcggtcaa catgatgctg ttgcaggcgt ggccttcccc
     4981 tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc ggtgctggtg
     5041 cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc agtggtcagc
     5101 gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga tgaaaacagc
     5161 gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca aattaccggc
     5221 gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc ccataaaacg
     5281 catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca catggacatg
     5341 aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca aagcggtggc
     5401 ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg gttgccactg
     5461 gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc gtttttctac
     5521 aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact ttcaccgctc
     5581 gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc cgaacgtatg
     5641 ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa agctgaagcc
     5701 gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc gggcgattta
     5761 cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt gtttgtctgg
     5821 cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa gtatctgctg
     5881 gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa
     5941 gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt gacgctcgac
     6001 ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc cacctggtat
     6061 gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct ttctgcggcg
     6121 gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat tgccaaagca
     6181 ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt acaaccactg
     6241 ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc
     6301 gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga gcgcgactac
     6361 cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact tggcaatggc
     6421 ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa actcaattac
     6481 accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat tgacgcttca
     6541 gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa agcgtggcag
     6601 gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa agaggacgag
     6661 aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag ccccacatgg
     6721 tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt tcatgagtta
     6781 attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc gtggatgcgt
     6841 gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag cgtcagtgag
     6901 atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct gacgccgcac
     6961 cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac gctctctcgc
     7021 ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc aggaacgcat
     7081 tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata actcggtcac
     7141 ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg cctggggctt
     7201 taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc gcaagatgaa
     7261 gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga aaaaatgaaa
     7321 atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg ccatacctgt
     7381 tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc atggtttaac
     7441 aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca ggaagagtgg
     7501 caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg cagcaagatg
     7561 ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga tgattactac
     7621 gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa acatatccct
     7681 actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat ctggggccca
     7741 aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg caacttcgag
     7801 gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta cctgccgcgc
     7861 ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg cgctatctac
     7921 aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg ctggcgtttg
     7981 tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg caagtcagaa
     8041 aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg ctcagaaacc
     8101 tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg catcgaggaa
     8161 gcggcgagca ccgagcgcga agttgacctc
//
LOCUS       DAJTNM010000151         8163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTNM010000151 DAJTNM010000000
VERSION     DAJTNM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.151"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(67..606)
                     /locus_tag="OO172_003627"
     CDS             complement(67..606)
                     /locus_tag="OO172_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5513644.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(671..1219)
                     /gene="fimA"
                     /locus_tag="OO172_003628"
     CDS             complement(671..1219)
                     /gene="fimA"
                     /locus_tag="OO172_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5513645.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1700..2296)
                     /gene="fimE"
                     /locus_tag="OO172_003629"
     CDS             complement(1700..2296)
                     /gene="fimE"
                     /locus_tag="OO172_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5513646.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2774..3375)
                     /gene="fimB"
                     /locus_tag="OO172_003630"
                     /pseudo
     CDS             complement(2774..3375)
                     /gene="fimB"
                     /locus_tag="OO172_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            4829..5545
                     /gene="nanC"
                     /locus_tag="OO172_003631"
     CDS             4829..5545
                     /gene="nanC"
                     /locus_tag="OO172_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5513647.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5565..6671
                     /gene="nanM"
                     /locus_tag="OO172_003632"
     CDS             5565..6671
                     /gene="nanM"
                     /locus_tag="OO172_003632"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5513648.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNRWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKEEYYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6736..7716
                     /locus_tag="OO172_003633"
     CDS             6736..7716
                     /locus_tag="OO172_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5513649.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2422 a         1745 c         1699 g         2297 t
ORIGIN      
        1 tttccttaca ttgacgtttt tattactcac tgtcctgttc ctgttatcac attatctact
       61 gaacaattac tgataggtta aagagaacca ggcctgggca ttggcgatgc cgccagtaac
      121 ccgacgcccg gtagcacgat atttggcgat gaaatgtagc gaagtagagc ctgagtaaag
      181 ccgtttccag tttgctggag gacgattaat cggtacgagg tttccttcat catcaaacaa
      241 cgctacacca atattggagg ctatccctgg cccctctccc acggagagca catccggatt
      301 tttaccatcc gcgacaccgt gaaacgccac acccacacgt tcactcacca ccgtgctaca
      361 ttcccgtaaa tgaataacaa aaggcaccgg tgcgctatct tcccctgccg catgaaaccg
      421 gttactgctg atttgcccca tattgaccgt catttgttta tcaccggctt caatccggca
      481 agtttccgca ataatgacgc cctgaaattg catatttccg ccgggcagcg tggtattcca
      541 tttatttccg gccagagcaa acattggcag caacgacgct aacagaaata atctcttcct
      601 tttcatcgtt atttttatcg cacaagggtg ggcatccctg cccgtaatga cgtccttgaa
      661 cctgggtagg ttattgatac tgaaccttga aggttgcatc cgcattagct gcacccgggg
      721 ttgcctcgcc gattgcataa taacgcgcct ggaacggaat ggtgttggta ccgttattca
      781 gggttgtttg tgcactaaat gtcgcaccgt ccagcgtcag agcattgcct gttctgtcca
      841 ggatctgcac gccaacgttt gttgcactac ctgcagcaga actctgcaga gctagtacat
      901 cagtacgcgt agcatcaatt gccgtaccta agaaggcaac agcggctttt gtggcaacag
      961 tggtatcgca atcattcagc tgaatgttaa aaccaacggc agagctggtt gctccagcct
     1021 gcttcaggct agcggtacga acctgtccca actgaacggt ttgatcaaca gagcctgcat
     1081 caactgcgca agcggcgtta acaacttccc ctttaaagtt aatggtccca ccatttaccg
     1141 tcgtagtatc ggccagagcc gctgcggaac tgagggacag agccgacaga acaacgattg
     1201 ccagagtttt aattttcatg ctgctttcct ttaaaaaaac tatttctaaa tcgacatggg
     1261 cagtcgttct gtacactttg ttttgtcaac gagtttgact gccaacactg cacagttttc
     1321 ccccaaaaga tgaaacattt ggggccattt tgactcatag aggaaagcat cgcgcacaaa
     1381 ctttttcagt ttatttgttg gcttaatatt ctattgttat ctttatttat agatttttat
     1441 attgcatgag gtggtttttg gagagaagaa tgaggaagat gcgtcgagcc acagaaacgt
     1501 tagctttaca tatagcggag gtgatgtgaa attaatttac aagagaaata atttacatat
     1561 caaatagtta gatgcttttt gtcgtttttt aatattttta tgcttgagaa aaaatacgta
     1621 acttatttat gatatggaca gtttggcccc aattgtcttg tgtttatttg ttttttaact
     1681 ttattatcaa taagttaaat caaacctctt ctctttttaa tttttcgttt atgagattat
     1741 ttctttccca taatccggca aaacgagcag cattactggc ggtataacgc acagtatggc
     1801 gaatatttcg atgcccgaga taatcctgaa ttaaacgggt atctgcacca cgctccgcca
     1861 gttcataacc gcaagcatgc cttaacatat gaggatgagt ctgcgttacg gttccagctt
     1921 caataccggc atcgcgaata atgcgatagg cctgctggcg agaaagccga ctcccgcggc
     1981 gagaaataaa tatagcgtcg gtccggtcag cgcctttcca gttagcacgt tcctgggtcc
     2041 agcgttccac ggcttcacgc tcatcaaaac gtaacgggtg aacggtagaa aatccgttct
     2101 tcagtcggcg aatatttatt cgaccttcat taaggtcaag gtcctgataa tgcagatcaa
     2161 gcagttcact aatacgcatc ccatgccgat atgccaacag aataagacaa taatctctgg
     2221 ctcccgttgc cccgtaacaa accgcctgca tcatggcctg aacttcttta ccggtaagat
     2281 aacgacgttt actcacaata gtagtactcc taactgagat atatttgaat gaatacctat
     2341 aggaaacctc aatcggtcaa aattagccca atagataaaa agataaccac acacgaagtt
     2401 atgtggctat cagtcaatta cattaaacat catacaaaaa taaaatatca ctgatgtgac
     2461 aacatctatt ttcattgatt tgattcaata taacaataga ttacagagga ttaacttcca
     2521 gttacttaaa aaataaagaa aaaagtgtgg aaacaggaga atttaagtga tgtgtaaact
     2581 tatgaatgat tttgtttatt tattttgaag aacataaacc attagatttt gtatgggtat
     2641 ggttaactta atgtttatta tgataattaa gaatcacact tacaataaca tcaattaata
     2701 aatggttaat catttttgat atatcgtaag aataatgtag tttttaacac catccctggt
     2761 atctcaacta tctctataaa acagcatgac gctgtcgtcc tctggctcta tcccagatgc
     2821 cgtaaaaacg ccctgcattg ctggcggtat accagacagt atgacgaata ttgcgatgcc
     2881 caagataatc ctagataagt cgcgtatcta ttcctatatt cgccaaagca aaaccacacg
     2941 aatagcgtaa catgtgcgga tgaatctcca gtgacaaccc ggcattacca ccagaagtcg
     3001 agataatatg gtaaaactgt tgccgagaaa gcggattccc cttacgtgat aaaaataccc
     3061 actcgctctc agcatgcgga taagaagtac ggatactcaa ccagtttttt aaagcctgaa
     3121 cttctttgtt caatagcggg tgcgttgttg aaaagccttt ttttaatcga tggatatata
     3181 tacactttgc tttaagatca atatccgaaa tcctcaatcg acaaatttca ctcgcccgga
     3241 aaccatgaat aaaacaaagc aaaatcagac aataattacg tgttgcatga ggccggtatt
     3301 tgctgcttta aggagtgact cgatttcact atgggtaagg aagttccttt ttttgttatc
     3361 agccttattc ctcatcgttt tcccttataa ttacagacgc gcactagctg tgctgggtta
     3421 gcaataatcc aacattttta tcctgattat gtttataaaa gcgaacgttt gcttaataac
     3481 ttacgtaagt ggaccagttc ttctgagcga acttaaatgg agtagcgata gttacttata
     3541 ataactttgc catacaaacg ccatgctctt gcatgctatg tacctttata tatttatcaa
     3601 tctgatacga atctaataat aaccctcatc attaatgcaa ctaatcttat ctatatatca
     3661 tgtgatatgt tctgtaacaa gtaaaatcaa cataaaaaat tcagcaaaat ttaatatttg
     3721 ttcaactagc cgtctcaaat atgacattat tagttaactg catttgccac aaaatggcta
     3781 tttcatggaa cgcagtaatt tcttagtggc tattatcatg ctaattctta gatccctagc
     3841 gattattcct gcctggttcg tcagcttagg ccatgagatt gttacattcc tttttatcct
     3901 ggagatatgg gggaacgcag gcgacgcttt ttcatctaca tccgttggta aatatagctt
     3961 tggtcactac gacttaagcc ttcctcaatc acagctattc gccgggcgat acggcaaaca
     4021 aactaagtga aacatcatcc tgaaaacacc aacatcaaca agcctctcca gatcgacatc
     4081 aggagtgacc agttacaagc cacaaaccaa gaaacatatt tcatgatgag aattatgctc
     4141 agcctgatgg cgagtaaacc cttattttca ccactctcac tgagttagct tgcaaatctt
     4201 tctttgttta gattctaaat aaatttttta taaaacatat agttaaaaga aaatctttag
     4261 tttttgaaga ccaccgcaag tgttcgtctg gcttcatatg gcatcttctt cttagaaaaa
     4321 gatcgacata ttttgtgacg cgaattgcaa atctggtttt gttgtatgga ttgcgtgatt
     4381 tttgatctgg tataacaggt ataaagatgc accacgatag tcaatgagac tgggcatctc
     4441 gtaatctatg gcaaacatca cttcagttct ttcacatcgg gtgatgaaaa tgcacttcag
     4501 tctgaaagga atatgaaaat gagatcaaca gacactctat tttatgactc tgggtaaaat
     4561 gggttgagta agtgatataa cttacgaata ttcaaatcaa ttaaaaatca gaagagattt
     4621 tatactcagg tatttaatct ggatctctgt ttatttaaat aatgttaaaa gagaattttc
     4681 acaggagacc ttatacaaaa aatataaaat acagttaccc gttgccaaag acactataag
     4741 cctggcaaaa aaatattaca caacataaat gctaattgtt tatgcgggct ttgtattgct
     4801 ttctgtatcc tacaaatgag tgaaatttat gaaaaaggct aaaatacttt ctggcgtatt
     4861 attactgtgc ttttcgtccc cattaatttc tcaggctgcg acactggacg tacgtggtgg
     4921 atatcgtagt ggaagccacg cctatgagac tcgactcaaa gtcagtgagg gatggcaaaa
     4981 tggatggtgg gcaagcatgg aaagtaatac ctggaatacc attcatgata ataaaaagga
     5041 aaatgccgca ctcaatgatg ttcaggttga agttaattac gcaattaaac ttgatgatca
     5101 atggacggtg cgcccgggaa tgttaacgca ttttagcagc aacggcacac gctacggacc
     5161 ctacgtaaaa ctgtcctggg acgcgacaaa agatcttaat tttggcattc gctatcgtta
     5221 cgactggaaa gcttaccgac aacaagactt atccggtgat atgtctcgtg ataacgttca
     5281 tcgttgggat ggatatgtca cttaccatat taatagtgat ttcaccttcg catggcaaac
     5341 gacgctatac agcaaacaga atgattatcg ctatgcaaac cacaagaaat gggcgacgga
     5401 aaatgccttt gttctacaat accatatgac gcccgatatt acgccataca tagaatatga
     5461 ctaccttgac cgccagggtg tttacaacgg cagagataat ttatcggaaa acagttatcg
     5521 cattggtgtg tcatttaaac tgtagtagac aggagacagt cacaatgaat aaaactataa
     5581 tggcgcttgc tatcatgatg gcttcatttg ccgcaaacgc gtctgtatta ccggaaactc
     5641 ctgtgccatt taaaagtggt accggagcaa ttgataacga cactgtctac attggtttag
     5701 gtagcgcagg tacagcatgg tacaagctag atacacaggc caaagataaa aaatggactg
     5761 cgttagctgc attccctggt ggaccgagag agcaagcaac ctctgcattt attgatggca
     5821 atctgtatgt gtttggcggc attggcaaaa acagcgaggg attgactcag gtatttaatg
     5881 acgtacacaa atacaacccc aaaaccaatc gctgggttaa attgatgtcg cacgcgccga
     5941 tgggcatggc gggtcatgtg acttttgtac acaacggcaa ggcttatgtt actggcggtg
     6001 ttaaccagaa tatcttcaat ggctattttg aagatctcaa cgaggctgga aaagattcaa
     6061 ccgctataga taaaatcaac gcccactatt ttgacaaaaa agaagaatat tatttcttca
     6121 ataagtttct gttgtctttt gatccctcaa cacagcaatg gagttacgct ggcgaatcgc
     6181 cctggtacgg aacggctggt gcggcggttg tgaataaagg tgataaaacc tggcttatta
     6241 atggcgaagc caaaccagga ttgcgaacgg atgccgtatt tgaacttgat ttcaccggta
     6301 ataatttaaa atggaataag cttgatcccg tctcatcacc agatggcgtc gctggcggtt
     6361 ttgcggggat aagcaatgat tctcttatat ttgccggagg ggccggattc aaaggttcac
     6421 gagaaaatta ccagaacggt aagaactatg cgcatgaagg cctgaaaaaa tcatatagca
     6481 ctgatattca tctttggcat aacgggaaat gggataaatc gggtgaatta tcgcaaggtc
     6541 gggcctacgg agtatcattg ccctggaata atagtttatt aattattggc ggtgaaactg
     6601 caggcggcaa agcggtgacg gattcagttt taatttctgt gaaggataat aaagtcacag
     6661 tacaaaacta acgcttcagg gccccggtaa aggggccctg ctatcaattt gtcatttgta
     6721 ttacgcgaaa attatatgaa cgcaataata tcgcccgatt attactatgt tcttaccgtt
     6781 gctggtcagt ctaatgccat ggcgtatggc gaaggactgc cattaccgga cagggaagat
     6841 gcgcctcatt ccagaattaa acaattagcg agatttgcgc atacgcatcc cggaggcccg
     6901 ccatgtcact ttaacgacat tattccactg actcactgcc cacacgatgt tcaggatatg
     6961 cagggttatc accatcctct ggcaacgaat catcaaacac agtacggcac cgttggccag
     7021 gcactgcata ttgcacggaa attactgccc ttcattcctg ataatgcagg gattcttatc
     7081 gttccgtgtt gccgtggcgg atcggctttt accgcgggca gcgaagggac atattcagaa
     7141 cggcacggag ccagccatga tgcttgtcgt tggggaacgg atactccgct gtaccaggat
     7201 ttagtcagca gaacgcgagc cgcactggca aaaaatccgc agaacaaatt cctcggcgta
     7261 tgctggatgc aaggcgaatt tgacttaatg accagtgact acgcgtcaca ccctcaacac
     7321 tttaatcata tggttgaagc ctttcgtagg gatctaaaac aataccattc tcagcttaat
     7381 aatattactg acgcgccgtg gttttgcggc gataccacct ggtactggaa agaaaatttc
     7441 ccgcattcgt atgaagctat ttatggcaat tatcaaaata atgttttagc caatattatt
     7501 ttcgtcgact tccagcaaca aggtgaaaga ggactgacga acgcgcctga tgaagacccg
     7561 gacgatttaa gcacgggata ttacggttca gcgtaccggt caccggagaa ctggacgacg
     7621 gcactgcgaa gcagtcattt cagcacggca gcccgtcggg ggattatttc tgacagattt
     7681 gtagaagcaa ttttgcagtt ttggcgcgaa aggtgagcgg ggagtttatt catgggggat
     7741 gtccttgttc gttctcagag tagacaacaa aaaacaaatg agatcaaggt aatatctctt
     7801 cacttgcggg ctcgaacagc aggcaattgc caatataata atctggcaca agggtgaaaa
     7861 acgcgcaccg cataaaccgt tgttattgct tacagattag ccggatgcct taatagacat
     7921 ccgcgccttt tcgattatgg ctcggaaatc tacgaacatc tacacagttt actggaacgt
     7981 tttgggccac agcgttcgca gcaccgtcct gatatgccgt tctggcgatt ataagtcgac
     8041 ggattctggc aaatagacga tgatgatatg cttaatgatg ttcaaagaaa ttaggccatt
     8101 attatattat tgcttgcatt ttctttcatt cctaaaagca cccgccatta atcccaggcg
     8161 cat
//
LOCUS       DAJTNM010000152         8159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTNM010000152 DAJTNM010000000
VERSION     DAJTNM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.152"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..320)
                     /locus_tag="OO172_003634"
     CDS             complement(<1..320)
                     /locus_tag="OO172_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="catalyzes the formation of AMP from
                     adenosine-3',5'-bisphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5513650.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALID"
     gene            510..2453
                     /gene="cpdB"
                     /locus_tag="OO172_003635"
     CDS             510..2453
                     /gene="cpdB"
                     /locus_tag="OO172_003635"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5513651.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(2579..2959)
                     /locus_tag="OO172_003636"
     CDS             complement(2579..2959)
                     /locus_tag="OO172_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5513652.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3048..3908
                     /gene="qorB"
                     /locus_tag="OO172_003637"
     CDS             3048..3908
                     /gene="qorB"
                     /locus_tag="OO172_003637"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5513653.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            4017..4982
                     /locus_tag="OO172_003638"
     CDS             4017..4982
                     /locus_tag="OO172_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5513654.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5090..5752
                     /gene="ytfE"
                     /locus_tag="OO172_003639"
     CDS             5090..5752
                     /gene="ytfE"
                     /locus_tag="OO172_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5513655.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(5797..7209)
                     /gene="cycA"
                     /locus_tag="OO172_003640"
                     /gene_synonym="dagA"
     CDS             complement(5797..7209)
                     /gene="cycA"
                     /locus_tag="OO172_003640"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5513656.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7517..8137)
                     /gene="fklB"
                     /locus_tag="OO172_003641"
     CDS             complement(7517..8137)
                     /gene="fklB"
                     /locus_tag="OO172_003641"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5513657.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALIAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         2076 a         2137 c         2067 g         1879 t
ORIGIN      
        1 tggtcaatga gcgcaatgtt aacggtgaat tcgccattac gtttaataaa ctctttagta
       61 ccatccagcg ggtctaccag ccagtaacgc tgccagtgct gacggacttc ccaaccggga
      121 ggatcttctt cagaaaggac cggaatatcc ggtgccagcg tacgtaaacc gtccatgata
      181 acggtgtgag cggcaatatc cgctgccgtt accggagaat tgtccgcttt gctgacgacg
      241 tccatcggtt tcgtcccgtc gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc
      301 tggcatactt gatctaacat ttctccacct cgtctctgtg agcggtgtta acttattgtt
      361 ttacttatac cctatcgtta atgaatgcgc caactgtgat agtgtcatca ttttcaaagc
      421 gtaaaattgt ggcattcttc actgttctat aagtaaggcg tttattctcc cttttctttc
      481 gtattcccga tgataaaagg atgtccctga tgattaagtt tagcgcaacg ctcctggcca
      541 cgctgattgc cgccagtgtg aatgcagcga cggtcgatct gcgtatcatg gaaaccactg
      601 atctgcatag caacatgatg gatttcgatt attacaaaga caccgccacg gaaaaattcg
      661 gactggtacg tacggcaagc ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac
      721 tggtcgataa cggcgatttg attcagggga gtccgctggc cgattacatg tcggcgaaag
      781 gattaaaagc aggtgatgtt catccggttt ataaggcgct gaatacgctg gattatacgg
      841 tcggtacact cggcaatcat gaatttaact acggtctgga ttacctgaaa aatgcgctgg
      901 cgggagcgaa attcccttat gtaaatgcca acgtcattga cgccagaacc aaacagccaa
      961 tgtttacacc gtatttaatt aaagataccg aagtggtcga taaagacgga aaaaaacaga
     1021 cgctgaagat tggctatatt ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta
     1081 atttatccgg aaaagtgacg gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg
     1141 aaatgcgcga gaaaggtgcc gatgtcattg tcgttctggc gcattccggg ctgtctgccg
     1201 atccgtataa agtaatggcg gaaaactcag tttattacct cagtgaaatt ccgggcgtta
     1261 acgccattat gtttggccat gctcacgccg ttttcccggg taaagatttt gctgatatcg
     1321 aaggggctga tatcaccaaa ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt
     1381 ggggcgatca tcttggggtg gtcgacttac aactcagtaa tgacagcggt aaatggcagg
     1441 tgacgcaggc gaaagcggaa gcacggccga tttacgacat cgccaataaa aaatccctcg
     1501 cggcggaaga cagcaagctg gtagaaacac tcaaagccga tcacgatgcc acacgccagt
     1561 tcgtcagcaa gccaatcggt aaatctgccg acaatatgta tagctatctg gcgctggtgc
     1621 aggacgatcc gaccgtgcaa gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca
     1681 ttcagggcga tccggatctg gcaaaactgc cggtgctttc agctgccgca ccgtttaaag
     1741 ttggtggtcg caaaaatgac ccggcaagct atgtggaggt ggaaaaaggc cagttgacct
     1801 tccgtaatgc cgccgatctt tatctctacc ccaatacgct gattgtagtg aaagccagcg
     1861 gtaaagaggt gaaagagtgg ctggagtgct ccgccggaca gtttaaccag attgctccta
     1921 acagcacgaa accacagtca ctcatcaact gggatggttt ccgcacttat aactttgatg
     1981 ttattgatgg tgtgaattat cagattgatg ttacccaacc cgcccgctat gacggcgagt
     2041 gccagatgat taatgccaat gcagagcgga ttaagaacct gacctttaat ggcaaaccga
     2101 ttgatccgaa cgctatgttc ctggttgcca ccaataacta tcgcgcttac ggcggcaaat
     2161 ttgccggtac gggcgacagc catatcgctt ttgcttcacc ggatgagaac cgctcggtgc
     2221 tggcagcgtg gattgctgat gagtcgaaac gtgcggggga aattcatccg gcggcagata
     2281 acaactggcg tttagcaccg atagctgccg ataagaaact ggatatccgt ttcgaaactt
     2341 ccccgtcaga taaagccgca gcgtttatta aagagaaagg gcagtatccg atgaataaag
     2401 tcgcgaccga tgatatcggg tttgcgattt atcaggtgga tttgagtaag taaaacactt
     2461 ctttttcggc ctataaatca accgcatccg gcatttattg gcggatgcga tgctgccgta
     2521 tcttatccac cctacaagcc atgaaccgta gaccggataa gcgcagcgca tccggcagtt
     2581 atgccgcacg ttcatcccgc accgccaaca cctcgggcaa attcaactca atccagtccg
     2641 ccagtgcagc aaccttttcg ctcacctgct cgcccagcgg cgtgaggcta tattccacat
     2701 gcggcggcac caccggatac gcgatacggt taagaaaacc atcctgttct aacgcctgta
     2761 acgactgcgc aagcatcttt tcactcaccc caccgatttt gcgccgcagg tcgctaaagc
     2821 gatgagtacc ttcgcgtagc gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt
     2881 tcaacacctc gcgcgacggg cactgttccg caaagagatt accctctttc agttgttgtg
     2941 acaggctaac ctgactcatt tcatacttac ctttttgcac gtacttacta aaagtaagtt
     3001 taggtgttag cgtatttaaa cacaagacaa aacgatggag acttcccatg atcgctatta
     3061 ctggtgccac tggccaactt ggtcactata ttattgaatc cttgatgaaa acggttcctg
     3121 ccagccaaat agtggctatc gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag
     3181 gcattaccgt gcgtcaggct gactacagcg atgaagccgc actgacatct gcacttcagg
     3241 gagtggaaaa actactgctg atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc
     3301 gtaatgttat taatgccgca aaggcggctg gcgtgaaatt tatcgcttat accagcctgc
     3361 tacatgcaga tacctccccg ctcggcctcg ccgatgagca catcgagacg gagaaaatgc
     3421 tggctgattc tggcatcgtt tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc
     3481 tcgccagcgc cccggcagca ctggaacacg gcgtatttat cggtgcggcg ggcgatggca
     3541 aaatcgcctc agcaacgcgg gcagattatg cgacagctgc ggcactcgtg attagcgaag
     3601 ccggtcacga aggcaaggtt tacgaactgg cgggcgatag tgcctggacg ttgacacagt
     3661 tagcggcaga gctgaccaaa cagagcggca aacaggttac ctatcaaaat ctgagcgaag
     3721 ccgatttcgc cgcggcactg aaaagcgtcg gactgcccga cggactggcg gatatgctgg
     3781 cggattctga cgttggcgca tcgaaaggtg gtctgtttga taacagcaaa acgcttagca
     3841 aattgattgg ccgcccaacg acaacgttag ccgaaagcgt aagccatctt tttaatgtta
     3901 ataactagtt aattaaagtg gcatcctccc gcatcctctc tgataatgac gggatgccgg
     3961 gagcaatcat gtctgcttcc tgaacgtttc ttctgacaga ccaatggatg ccagtaatga
     4021 ttagcggcgt gctgtacgcc ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc
     4081 cgttgatcgt gccggaatac ccggcgatgt tacagtcgat ggggcgttat ctggcgttag
     4141 ggttaattgc gctacccatt gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc
     4201 gggactggct taccgccttg atgctcacta tgatgggcaa cctcatttat tacttctgcc
     4261 ttgccagtgc cattcaacgt actggcgcgc ctgtttccac gatgattatc ggcaccctgc
     4321 cggtggtgat tcctgtcttt gccaatctgc tttatagcca gcgcgacggc aaactcgcgt
     4381 ggggaaaact cgccccggca ctgatttgta ttggcatcgg cctggcgagt gtgaatattg
     4441 ctgagttaaa ccacggactc cccgattttg actgggcacg ttatacctct ggcatcgtgc
     4501 tagcgttagt ttccgtggtc tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc
     4561 gggaaaatcc cgacaaacat ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc
     4621 cggtttctct catcggctat ctcgtcgcct gttactggct gaatatacaa acgccggact
     4681 tctccttacc ttttggcccc cgtccgctgg tgtttattag tctgatggtt gcgatagccg
     4741 tgctttgctc atgggttggc gcactctgct ggaacgtcgc cagccagcga ttaccgacag
     4801 tgattctcgg gccgctgatt gttttcgaaa cgctggcagg tttgctgtac acctttttac
     4861 tccgccagca aatgccgccg ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg
     4921 tggtcatcgc ggtcagagca aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt
     4981 gacatgctgg cagtgagtta aataagcctc tgctacgtaa gggttatagc ttttgcctta
     5041 aaggtgtatt taaaatacat cttatcttat taagaatgag gtatcagcta tggcttatcg
     5101 cgaccaacct ttaggtgaac tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa
     5161 atatgatatg gattactgct gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa
     5221 agaactggat gttgaggtca ttgaagctga actggcaaag cttgctgaac aaccgattga
     5281 gaaagactgg cgtagcgccc cgctggcaga aatcattgac catatcatcg tgcgctacca
     5341 cgatcgtcac cgcgagcagc tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt
     5401 tcacgccgac aaaccgagcg tgccaaaagg gctgacaaaa tacctgacca tgctgcatga
     5461 agagctttcc agccacatga tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg
     5521 catgggcagc caggcaatgg ggccaatcag cgtaatggaa agcgagcacg atgaagcggg
     5581 cgaactgcta gaagtgatca aacacactac caataacgtc acaccgccgc cagaagcctg
     5641 caccacctgg aaagcgatgt ataacggcat taatgaactg attgatgacc tgatggatca
     5701 catcagtctg gaaaacaatg tactgttccc acgcgcccta gcgggtgagt gatctaaaag
     5761 ctggatggca ttgcgccatc cagcatgata atgcggttat ttccgcagtt cagcagcccg
     5821 cttcttacca ataaacagcc agcccaaccc cagcgcgata aaccacagcg gggtaaccag
     5881 cagcgcctgg cgagtgtcat cttccagtgt cagcaacacg accacgaaca caaagaacgc
     5941 catacatacc cagcacatca gcttgccgag cggcatcttg tagatcgact tttcatgcag
     6001 atgaggacgc tgtttgcggt acaccaggta cgagcaaagg ataatcgtcc agacgaacat
     6061 aaacagaatc gcggaaacgg ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac
     6121 atacaacatc accacgccgc cgagcagaca gatacacgag aaggtcaacc ctttcgcggg
     6181 tactgcgcgc ttagaaagtt tagcgaacgc tttcggcgca acaccttcct gcgccagacc
     6241 aaacagcata cggctggtag agaagacacc gctgttagcg gaagacgccg cagaggtcag
     6301 caccacaaag ttgatcacgc tggcagccgc aggcaaacct accaacacaa acagctcaac
     6361 gaacgggctt ttctccggga ctaccgaact ccacggtgtc acggacataa tcacaatcag
     6421 cgcgaagacg tagaacataa tgatacggat cggaatggag ttaatcgcgc gtggcagtga
     6481 tttctccgga tctttggttt ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc
     6541 gaaaaccgct atctggaatc cggcaaagaa gccacttaaa cctttcggga accagccgcc
     6601 gtcattccac aaatgcgcga atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc
     6661 caccatgacc aggccgacga caatcagcga cacgatggcg acgattttga tcatcgcaaa
     6721 ccagaactcc atctcaccga acattttcac ggtggcaagg ttgagcgtca gcagcagcac
     6781 tatcaccgcc agcgaggcga cccagtcgga gaaatcgggg aaccagaact gagcataagc
     6841 cgtgatcgcc accacgtctg ccataccggt tacaacccag cagaaccagt aagtccagcc
     6901 ggtgaaatat cccgcccacg gccccagtaa atcggaagcg aagtcactaa aagatttgta
     6961 ttccagattc gaaagcagca attcccccat tgcccgcatc acgaaaaaga gcataaaacc
     7021 aatgatcata taaacgaaaa tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc
     7081 cataaacaac ccagtaccaa tggcaccgcc aatggcaata agctgaatat gtcggtttgt
     7141 gagattgcgc cgtagcgact gttcagccgg agcctgatca tcggcaacga cttttacctg
     7201 atctaccatg ttttttcttc ctgtacctgt ctgtgttgtt caggctctac ggcctttagt
     7261 cagtctatga tacgacgaat ctgtatcggg actcatcgat attaggtaac aatacgcggg
     7321 atgaatacta agatttagat ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg
     7381 gtgataacgc gaaacagctc acaaaaatac acgcttacgg ggtctttgca acacaaaata
     7441 gcgccgtttg gcaaactgaa gggtttattg ctgaatgcct gctcccctct ggttcgaggg
     7501 gaacagaatg cgctgcttag aggatttcca gcagttcgac ttcaaacacc agggtgctga
     7561 acggagggat ggatgcacct gcgccgcgct cgccatatgc cagttcctgc gggatagtca
     7621 gttcccattt agaacctacc ggcatcagag tcagtgcttc aatccagcca gggatcacgc
     7681 cattaaccgg gaattcagcg ggttcaccac gagcaacgga gctgtcaaac acggtgccgt
     7741 cgatcagttt accggtgtaa tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt
     7801 caccttggtt gatcacgcgg aattgcaggc cagattcggt gctattcaca ccttcttttt
     7861 tggcgttttc ttccaggtat ttcacacctt cagcagccat cgcctggaaa cgctgacgac
     7921 gaacggcatc ggcgcgttcg tggatttcac gcagcgcgcg atgcaccaca tcaaccggca
     7981 cagccggatg tttgccttcc agcgcatcgg caatacctgc aatcagtgct tctggcagca
     8041 gcccttccag gccagattca ctcagttgtt gccctacctg caaaccaatg ccgtagcttg
     8101 cttgcgcttc gatggtgtca aaagttgggg tggtcatcgt atttcctttc ttagggtat
//
LOCUS       DAJTNM010000153         8144 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTNM010000153 DAJTNM010000000
VERSION     DAJTNM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.153"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..893)
                     /locus_tag="OO172_003642"
     CDS             complement(<1..893)
                     /locus_tag="OO172_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5513658.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            953..1231
                     /locus_tag="OO172_003643"
     CDS             953..1231
                     /locus_tag="OO172_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513659.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1232..1759)
                     /gene="gadE"
                     /locus_tag="OO172_003644"
     CDS             complement(1232..1759)
                     /gene="gadE"
                     /locus_tag="OO172_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5513660.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2558..3130)
                     /gene="hdeD"
                     /locus_tag="OO172_003645"
     CDS             complement(2558..3130)
                     /gene="hdeD"
                     /locus_tag="OO172_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5513661.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3385..3717
                     /gene="hdeA"
                     /locus_tag="OO172_003646"
     CDS             3385..3717
                     /gene="hdeA"
                     /locus_tag="OO172_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5513662.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3833..4159
                     /gene="hdeB"
                     /locus_tag="OO172_003647"
     CDS             3833..4159
                     /gene="hdeB"
                     /locus_tag="OO172_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5513663.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4223..4869
                     /locus_tag="OO172_003648"
                     /pseudo
     CDS             4223..4869
                     /locus_tag="OO172_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4911..5441)
                     /locus_tag="OO172_003649"
     CDS             complement(4911..5441)
                     /locus_tag="OO172_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5513664.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5597..6163)
                     /gene="slp"
                     /locus_tag="OO172_003650"
     CDS             complement(5597..6163)
                     /gene="slp"
                     /locus_tag="OO172_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5513665.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6410..7633)
                     /locus_tag="OO172_003651"
     CDS             complement(6410..7633)
                     /locus_tag="OO172_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513666.1"
                     /translation="MSIDFTLGIINTYHGDIYNCTTNTDNVKTPDTPKWPYDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2366 a         1576 c         1703 g         2499 t
ORIGIN      
        1 cggctacctt catccactaa tgccgtgaca tacatgccag gcagcaagtc accatttggg
       61 ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catcaaccgt cgggtcggag
      121 aatttcagcg tgccggtctg gctgtaacgt ttaccatttt ccagattgag ctgtactggc
      181 gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat gcgtaagaaa
      241 tcttgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc
      301 gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt cccgctgacg
      361 cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc
      421 gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc ggtgtcgtaa
      481 tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg
      541 gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc
      601 tgtaaaggtg caggatcaat ctgatacagc gaatcgctct ggttcacttt atcgccttcg
      661 ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc agcttcataa
      721 ggaacggttc taccgggcaa ttcgctcaac acattgaccg aactagggga gagtgtgacg
      781 acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc gcaggcggtg
      841 agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttagtc
      901 cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga
      961 tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca ggcacagtga
     1021 tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag
     1081 cattcgggca acgaagggtt ctactggtgg atacacatac caggggaatc aagtcgtttt
     1141 agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc
     1201 cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga
     1261 cgatgtcgct catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta
     1321 atgtttgttg cgaaatattg agcgtcgacg tgatattgct ttcattttta tttttcagaa
     1381 tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc ttatggggca
     1441 agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat
     1501 aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct
     1561 cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg
     1621 tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga
     1681 tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt
     1741 tcgtcatgag aaaaatcata acttgctcct tagccgttat tgtttcgaac atgtcatcct
     1801 tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact
     1861 ttgcccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca
     1921 cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa
     1981 acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa ccagtttgtg
     2041 atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg agtctccttg
     2101 tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga aattacctga
     2161 gtaaataacg catttatgtc ttcaggtttt tatatagcgt ctggagacac ggatatttat
     2221 gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat
     2281 agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac
     2341 ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga
     2401 aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt atttcaatga
     2461 tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg atcatagtca
     2521 catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta acgaacaaac
     2581 tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa
     2641 ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca
     2701 gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca
     2761 tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa
     2821 tgcccagctc cggtgcacgg atgaagaaat agccgatcaa taaataggcg actgcgacga
     2881 ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac ccgacaataa
     2941 gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata tcgccagaga
     3001 cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca ataaactgga
     3061 ttgctctgcg atgctttttc agcatctcca gatcaaactt caaaattgtt gccttatcta
     3121 tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat caatctatgc
     3181 caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc agtatctgat
     3241 gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt ccatcaacat
     3301 gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgattcgtg acggctcttt
     3361 cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct tggtggtctg
     3421 cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa
     3481 ccggtcaact catggacctg tgaagatttc ctggctgtgg acgaatcctt ccagccaact
     3541 gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc ggttttagat
     3601 gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca ggataaacaa
     3661 gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc
     3721 cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat cttctttaaa
     3781 agcagctatt cctcctgttc atatataatc tctatattga atgggttaca aaatgaatat
     3841 ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt cactggtgaa
     3901 cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc aggaatttat
     3961 tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg aagaaacagt
     4021 atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt
     4081 gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa atcaagcatc
     4141 taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg agccatcttg
     4201 ctgctccttt ttgcattttt atatgacagc agaattaatt atacgtctta tactcgcggc
     4261 aattgcctgt ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg
     4321 cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga aatatggttt
     4381 tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg ctgctcaggt
     4441 ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc aaaatattgt
     4501 aggtctgacg acggcagcgg atatctgggt gaccgccgca taggtatggt tattggcagc
     4561 ggtatgtacg aactgggtat ttatggttca gtgatgacct tgttggtgct ggaagtcttc
     4621 catcaattaa ccttccgcct gatgaacaaa aattaccatt tacagctgac attagtgaac
     4681 ggtaatacgg tatcaatgct cgactggttt aagcagcaaa aaataaaaac tgatttagtt
     4741 tcgttgcagg aaaatgaaga tcatgaagtg gtcgccattg atatccagct tcatgccaca
     4801 acgttggtag aagacctgtt gcgattatta aaaggtatgg caggggtgaa aggcgtttca
     4861 attagctaat ctgtgtggca gcgtagccag actcaccgta agcctgaaat tcacaccaga
     4921 taatcaatat gctgatggcg taccagctca ttgatccgtt taagattgag gatcatcatg
     4981 atattgtgtt tatggcagta gaacgttttt agcgatattt tcagtttctc cagaatatct
     5041 tcagttgtca tttgattcgc aatgcagctc agaacctgat gttgcttgcg cgaaagtgac
     5101 acattcgctt caggcacaga gcagtaactg ccattgatga gtgcgacaaa atcaatgata
     5161 gtgatagtcc gcggaacaaa ggtcaccggt actttcccca gacagcgttt aatattaaag
     5221 ggggagaaaa caatgacatg gacaggtttt aatcgttcaa tttgagtgag aaaattagaa
     5281 tgaaatacat tattcattaa tgtatcaata atgacgaagg cattctgatg caacattacg
     5341 tcgatctctt cttcgttctg tatatgaacg acattacctt tactcagaat gtttttcatc
     5401 gcggtgaaga acatcgtatc cctggtaatt ataagaaaca taaccgactc ctggtgcgga
     5461 cattaacttt ataatcagtt tgccaaaggt aattaacagg acagggctta taataataat
     5521 agttagctta ctgtatatta attttatgta atacatgatg cagatgctga gaaagcatct
     5581 gcatctttcg gtggtgttat ttgaccagct caggtgttac ctgactcacc gcattggtgt
     5641 agtaaggcgc acccaagccc ggttccggcc agaatgcacc atagccgtaa tcccacaggt
     5701 tataggtggt attaaccact tctctcaaat gccacacctg gatgccctgc atattcactt
     5761 ccaggaagtt atacgggact ttattgataa agcctggttg ttcaccctga atggtaccga
     5821 ggatggtaac aaagtgatta cgatagttca ctggatcaag gaagccgctt tgtctggcga
     5881 gcagtcggcc ctgatagttg gcttcaatat caggcttcgc atagctatcc aacggtaata
     5941 cagcgatttc taacaacgta tccgttttgc cattgataac gttgataacc ttcccaccaa
     6001 agcgcgcttg ttgaccaaca tataaccccg gctggttatg aacagcaaca aaactttttt
     6061 gaatatcagg ttggttattg cctttgatat tttgcggaat tgaactacat gcggcaagca
     6121 aaaatgaaag gctgaggatg agtgcacctt ttgtcatgtt catgttacta tccttatcaa
     6181 caaatattaa aaccataata gatatttctt tatagttttc atctgattct gaggtgatat
     6241 gcctgatagt aacgtaaacc tgtctgatgt ttatatccct tctgtttagt tcaattccgt
     6301 tattttgggg cgtggtatga gtatccatcg cccaataata taaccctttg ttttaaaatt
     6361 acataaaatc tatttttatt caatgattat aaagtgctaa taatcaatat tatggtatta
     6421 tattctgata atgatttgat aagcgaattc ttttgccaga tggtatttct atttccaccc
     6481 cataatttat atctttttca ttaggcagat ctctctttaa ataaaagggg tatgaaatct
     6541 tttcgggttg aagtgtctcg taatgaaatg ctatgaattc atttttagac attgttgccc
     6601 atttttgaat tttattgtat agagagggtg ttgtaaactc ttgaagttgt ttataacata
     6661 tttttttgaa ctcttgctta tattttaata tgtcatcaaa tgaaagattc actacctttg
     6721 cctctaagaa atcaattatt ctcgaagcaa tttccgcttt cgcgcgtaaa gagttgtcat
     6781 taatagttaa caatgtagaa aacaagccat tgatttgttt gtcaagatgt tgttcatatt
     6841 tatgtagttt ggggatggga ttttccttgt gtgaaaaaat agtgttaaat aaatttgcgt
     6901 cagtgaaatt tatattaagt agatcttcaa aataaaattt tgataaggtt aggactagct
     6961 tggacatatc ttgccctgtc atgttgaaat caataaagtc gcatccagca agggcttcca
     7021 tcctattttc atttttactg tgctcaatat acagcttcgc catttttaag tacaagcttt
     7081 gaatgtctct tatatcggtt ggtaagtcag ctcgttgccc agcaaaattt ttattatcgg
     7141 aagaaacggt ttggacactg aatctttcgg gtattccttc ttctacgaga attttagtta
     7201 tgattggatt gttgggttct ggattaacga caacaatgat ttgacataca gaatccttat
     7261 ccttttcgta tctaatgtta tgaatctgat taagtattga ttgtgtctgt tgcgtaagtg
     7321 gtgtcgaact tatcgtttca atgttattaa taagacttac aagttgaacc acaacttttg
     7381 agtagtctgc atttgtatat gtggtgttcg tgtggcatgc attaatttgt tggagttgtt
     7441 gttgcgctaa atcaaattgt tcagggttgt atccttctcc agagaatgtg gaattaatgg
     7501 gttgttgttc ttcccaatta tcataaggcc acttcggtgt gtctggcgtt ttaacattat
     7561 cggtatttgt tgtgcagtta taaatatcgc cgtgatatgt atttattata cctagggtaa
     7621 agtcaattga cataacattc cttattcatc atctgtttta tgctaatgtt tttaaattgg
     7681 ataattagaa caggaatgga ttttcttcta cacacaataa atataatgcg ttatatcttc
     7741 ctgatataat tacctttaaa ttccctttca tttccacccg gtaatatttg tgtataaaaa
     7801 aagggcagtg atagtatcgt aaaggaaaac gttaacaact atcaaaaagc gctcatttta
     7861 aaaagttatt atgttctccg atagtgtgtg aagtaatatt tttgtgatgt tttagatgtg
     7921 taataatgaa ccaggttaaa gtgtatttaa aaatgtgttg tattagcgac tgaaatctgc
     7981 cttttaaaaa ggaggagggt actgagacaa agagtcattg cttctccgat aaaatgtcag
     8041 agaagcatga ttacgtggtt attgtctgtt gatgacttgt tgaccgagcc gtcatcattc
     8101 tcaatgcatt agttaaatca tttgtgattt ttaaggatta tcct
//
LOCUS       DAJTNM010000154         8109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTNM010000154 DAJTNM010000000
VERSION     DAJTNM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.154"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            332..3181
                     /locus_tag="OO172_003652"
     CDS             332..3181
                     /locus_tag="OO172_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5513667.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3281..5200)
                     /gene="dhaR"
                     /locus_tag="OO172_003653"
     CDS             complement(3281..5200)
                     /gene="dhaR"
                     /locus_tag="OO172_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5513668.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5428..6498
                     /gene="dhaK"
                     /locus_tag="OO172_003654"
     CDS             5428..6498
                     /gene="dhaK"
                     /locus_tag="OO172_003654"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5513669.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDVPVHTPALNWGK"
     gene            6509..7141
                     /gene="dhaL"
                     /locus_tag="OO172_003655"
     CDS             6509..7141
                     /gene="dhaL"
                     /locus_tag="OO172_003655"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5513670.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7152..>8109
                     /gene="dhaM"
                     /locus_tag="OO172_003656"
     CDS             7152..>8109
                     /gene="dhaM"
                     /locus_tag="OO172_003656"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5513671.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHH"
BASE COUNT         2111 a         2012 c         2027 g         1959 t
ORIGIN      
        1 cataactaat taggattatt acaaaaaatc ttcaccaaat tttttaaccg ctgtttgttg
       61 taatattttt gccatattca tagtaacgaa ttggaataag taacgacaac ttagggaata
      121 actcgatata aactcacctt tgtaaggggg gtgttaaata tatattaatt cctgtgttcc
      181 tgattttgcc cggtaataga gaattattct cagagaagct catcatgatt gcaccataca
      241 gatatcatga tgataagagg atattattca ttgacttaaa caaaatgaca aaatccatag
      301 acccatagcc tgggtactat ataggagtgg tatgggcatc aaacaacaca atgggaatac
      361 caaagccgat cgtctcgctg aattaaaaat ccgttcgccc tcaattcaac tgataaaatt
      421 tggcgctatt ggtttgaatg caattatctt ttcccccctg ctgatagctg ctgatacagg
      481 aagtcaatat ggcaccaata ttactattaa tgatggtgac agaattacag gagataccgc
      541 cgatccatca ggaaacctct atggtgtaat gaccccagca ggaaacacgc ctggcaatat
      601 caacctgggt aatgatgtca ccgtcaatgt caacgacgcc tctggatatg caaaaggaat
      661 cattattcag ggcaaaaaca gctccctgac agctaaccga ctcacagtag atgttgttgg
      721 tcaaacctct gccatcggca ttaacttaat tggtgactat acccatgctg acttaggcac
      781 aggcagcacc attaagagta acgatgacgg catcattatt gggcatagct caacactaac
      841 agccactcaa ttcaccattg aaaactcgaa cggtataggc ctaaccatca atgactatgg
      901 caccagtgtc gatcttggaa gcggaagtaa aatcaagacc gatggaagta caggtgttta
      961 tatcggtggt ctcaacggca ataacgccaa tggtgctgcg cgttttacgg cgacagacct
     1021 gacaatcgat gttcagggct acagcgccat ggggataaac gtacagaaaa actctgttgt
     1081 cgatctcgga acaaacagta ccattaaaac caatggcgat aatgctcacg gcctctggag
     1141 ctttggccag gttagcgcga atgcactcac tgttgatgta actggagccg cggccaatgg
     1201 cgtcgaagtt cgtggtggta caaccactat cggtgcagat agccatattt cttccgcgca
     1261 gggcggtggc ctcgtcacca gtggttcaga cgcgacaatc aatttttctg gcacggcagc
     1321 gcaacgaaac agcatctttt ccggcggttc ttatggtgcc tcggcccaga cggcaacggc
     1381 tgttatcaac atgcaaaata ccgatattac ggttgatcgt aatggcagtc tggcgctggg
     1441 tttgtgggcg ctcagcggcg gtagaataac cggagacagt ttggctatca ccggcgcggc
     1501 aggagccaga gggatttatg ccatgaccaa cagccagatc gacctcacga gcgatctggt
     1561 cattgatatg agtacacccg accagatggc catcgcaacg caacatgacg atggttatgc
     1621 caccagccgc atcaacgcct cgggtcgtat gcttatcaac ggtagcgttc tttccaaagg
     1681 tgggctaatc aatctggata tgcaccctgg gtcggtttgg acaggttcct ccctcagcga
     1741 taatgtcaat ggcgggaaac tggacgttgc aatgaataac agcgtctgga acgtaacaag
     1801 taattctaat ctcgacacgc tggcgctgag ccattcaact gtcgattttg ccagccacgg
     1861 gtcaactgcc ggcacattta ccacattaaa cgtagagaac ctgagcggta acagtacctt
     1921 tattatgcgt gctgatgttg ttggcgaggg taatggcgtt aataatagag gggatttatt
     1981 gaatatcagc gggagtagtg ctggtaatca cgtattggct atccgcaacc agggcagcga
     2041 ggccacaacg ggaaatgaag ttctgacagt ggtaaaaacc actgacggcg cggcctcgtt
     2101 cagcgcgtct tctcaggttg agttgggggg atatctgtac gatgtgcgta aaaatggcac
     2161 taactgggag ctttacgctt ccgggacagt tccggaaccg actcctaatc ctgaacccac
     2221 accagctccc gctcagcctc ccatagtcaa ccccgatcct acgcctaaac ccaccacgac
     2281 cgcagatgct ggcggcaatt atctcaatgt cggttactta ttgaactatg ttgaaaaccg
     2341 tacgctgatg caacggatgg gtgacttgcg aaatcagagt aaagacggta atatctggtt
     2401 gcgcagttat gggggaagcc tggactcctt tgccagtggc aaactgagcg gctttgacat
     2461 gggttacagc ggtatccagt ttggtgggga taaacgtctc tctgatgtaa tgccgttgta
     2521 tgtcggtctg tatattgact caacacatgc atcgccggac tatagcggag gcgacggtac
     2581 cgcacgttca gactacatgg gaatgtacgc cagttacatg gcacaaaacg gtttttacag
     2641 cgatctcgtt ataaaagcat cgcgccagaa aaatagtttc cacgtactgg acagtcagaa
     2701 caactgcgtt aacgccaacg gcactgcgaa tggaatgagc atctccctgg aagccgggca
     2761 gaggttcaac ctgtccccta ctggttatgg gttctatata gagccgcaaa cccagcttac
     2821 atacagccac cagaatgaga tggctatgaa ggcgagtaat ggcctcaata tacatctgaa
     2881 tcactacgaa tcgctgctgg ggcgtgccag catgatactg gggtatgaca tcaccgcagg
     2941 caacagccag ctgaatgtct atgtgaagac tggcgctatc cgcgagtttt caggggatac
     3001 cgaatatctg ttgaacgact cccgggagaa gtacagtttc aaaggtaatg gctggaataa
     3061 cggcgtggga gtcagtgcac agtataacaa acagcacaca ttctatctcg aagcggatta
     3121 cacgcagggt aacctctttg atcagaagca agtcaacgga ggatatcgct tcagctttta
     3181 agtgcgggat agacaagccc ttaaacgatg ctgaaaattg ttagcgtgcg ccggaatgaa
     3241 atacagatca ctctgatgta tcagaaatcg tttctgtctt tcatacccgg cgcttaaact
     3301 gccctgcatc aatgccatgt tgcttcattt tccgccacag cgtagtgcgg ccgatcccaa
     3361 gtaaagccga catttcctga atgcgaccgc ctgtgacctg ggctgcgtta ataattgcct
     3421 ctttttcaac ttccgcaaat gacagactgg tggaaaggcg ggtggcgctg acatcatctg
     3481 tcgcctgctc ggtaaacaga tgttcaggta aatcactgac gcgaatgcgc ccgttatcac
     3541 tactcagagc aagattctcg atgacgctgt aaagttcaaa atcgttgcct ggccatgcac
     3601 aagaaactag gcgagcgagg gcatcgtcat caattttcag ccgcgtagag aagcgttttt
     3661 caagactgcg taatttgtta ttcaccagcg ccggaatgct gccacgccgc atacgcagag
     3721 gcgggatggt aatttcaaat gcatgcagcg cgtaatacag ctggcgacta aaacgatttt
     3781 gttccaccag cattgcgagg tccgcggtcg ttgttgcaat cactttgaca tcaattggta
     3841 ttaaacgccg cgcatccagt cgcgtgataa ccccctgctt gataacctgc agcaaagcag
     3901 actgtaactc caccgccaga tattcaatct tttcaagaaa cagcgtgccg ccgtgtgcca
     3961 gttccagccg actcagacgg ccattttcat tgtccgtgcg atcgccacca ataaattctt
     4021 ccgccagcgc agcatcacca tataactcac aattgacggc gatataagga cctgcagcac
     4081 gctcgctttc attatgaatt gcctgactta gcagtgcctt gcccacgccc tcttctccac
     4141 aaagcaggac aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc aagcggcggg
     4201 tttgcggatc gtcctgtggc atatgagcga aggtatggct gacttttcct aattgactgg
     4261 tcattaactg ccgcatctgt tccacaggat ggagcaacaa aataaagctg gttccctgcg
     4321 tttctattat cggtttaagg gttatcaccg catcaataaa ctggtgctgg ctttcaaagg
     4381 ttgcttctac gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat acggcgggta
     4441 acgttaagag ttcagtgatt gcccgtccct gacttgccgt cgcgtcaagg cgcaagaccc
     4501 gcgccgcctg ggcattaata aattgcaaat taccctgctc gtcccagcta atcacgccat
     4561 catccatact ttctaacagg gcattaagtt gatttaaatg acggttagtt tcagcgagca
     4621 aactgtccgt cagcagtaaa ttaccgacct cgcgggcgat tgccaacgtc aacggcaaat
     4681 cagctgcggt agtttgctca accggacacg ccagcgctat tgttcccgtc aatcggccct
     4741 tgctgtcaaa caacggcgtt gcacaaaagg cccagttcca gagtgcctgt ttgaaatgtt
     4801 gatcggccat cgttttcacg gcctgaccag agatagccgc taacgatagc gcacaagtac
     4861 caataattcc ctcggcgcaa tacgtgccgt cattgaatcc cagtgcactt agctgctgca
     4921 aggtttgcgg atcgccatta cggctgagaa tgcaggcggt ttcatcgagg ataaacagcg
     4981 cacactctcg cggtgccata tattcccagg catcttccag cgcagcctgc ccgagcgtca
     5041 gcatcgcttt cttacgccga taaatagaag caaatgtcac gccctgggcc tgatgtggta
     5101 cgttccatgt ctcccgtttc atcagcttat tgcatcgctc ccaggaggtt gcaattaagg
     5161 gagatatgcc ccgaccatcg ttgttaaaag cgccactcat atcccgcatc ccttatgaca
     5221 aaaggtgttc cggctcgcaa ttttaagtat taatacacgt aaaagtttca atatggaaca
     5281 tttattaaag catatgtttc actatgaaac ataatttggg ctaattttct tttcccttgc
     5341 ctgatgcaca atggattcat cggcagtcag tggtcgccgt gtcgttgaac atcatccatg
     5401 ccctaccgta attgctggcg caaaataatg aaaaaattga tcaatgatgt gcaagacgta
     5461 ctggacgaac aactggcagg actggcgaaa gcgcatccat cgctgacact gcatcaggat
     5521 ccggtgtatg tcacccgagc tgatgcccct gttgcaggaa aagtcgccct gctgtcgggt
     5581 ggcggcagcg gacacgagcc gatgcactgt ggttatatcg gtcaggggat gctttcgggg
     5641 gcctgtccgg gcgaaatttt cacctcaccg acgcccgata aaatctttga atgctccatg
     5701 caaattgatg gcggcgaagg tgtactgttg attatcaaaa attacaccgg cgatattctt
     5761 aactttgaaa cagcgaccga gttactgcac gatagcggcg taaaagtgac cactgtggtc
     5821 attgatgacg acgttgcggt aaaagacagt ctttataccg ccgggcgacg cggcgttgcc
     5881 aacaccgtat taattgaaaa actcgtaggc gcagcggcgg agcgtggcga ctcactggac
     5941 gcctgtgcgg aactggggcg taagttgaat aatcaaggcc actcaatagg tatcgctctc
     6001 ggtgcctgta ccgttcctgc cgcgggcaaa ccttctttta cccttgcgga taatgagatg
     6061 gagtttggcg tcggcattca tggtgagcca ggtattgacc gccgcccttt ctcttccctt
     6121 gatcaaaccg tcgatgaaat gttcgacacc ctgctggaac atggctcata ccatcgcact
     6181 ttgcgtttct gggattatca acaaggcagc tggcaggaag aaccacaaac caaacaaccg
     6241 ctccagtctg gcgatcgggt gattgcactg gttaacaatc ttggcgcaac tccgctttct
     6301 gagctgtacg gcgtctataa ccgcctgacc acacgttgcc agcaagcggg attgactatc
     6361 gaacgtaatt taattggcgc gtactgcacc tcactggata tgaccggttt ctcaatcacc
     6421 ttactgaaag ttgatgacga aacgctggct ctctgggacg tcccggtcca caccccggcc
     6481 cttaactggg gtaaataagg agaaagcaat gtcactgagc agaactcaaa ttgttaactg
     6541 gctcactcgt tgtggcgata ttttcaacac cgagagcgag tatcttaccg ggctggatcg
     6601 cgagattggc gacgctgacc acgggctaaa tatgaaccga ggctttagca aagtggtgga
     6661 aaaactccct gctatcgcag ataaagatat cggtttcatt ctcaaaaata ccggtatgac
     6721 gctgctttcc agcgtcggtg gtgccagtgg tccgctgttc ggtaccttct ttatccgcgc
     6781 cgcacaggcg acccaggcac ggcaaagcct gacactggaa gagctttatc agatgttccg
     6841 cgatggcgcg gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata aaaccatgtg
     6901 tgatgtgtgg gtgccggtgg tggaatcgtt acgtcagtcc agcgagcaaa atctctctgt
     6961 tccggtggcg ctcgaagctg ccagtagcat cgccgaatcc gcggcacaaa gtacgattac
     7021 gatgcaagcc cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg gtcaccagga
     7081 tcccggcgcg acctcggtga tgtttatgat gcaaatgttg gcgttagccg caaaagagta
     7141 aggaattggt gatggtaaac ctggtcatag tttcacatag cagccgactg ggagaaggtg
     7201 tcggtgaatt agcccgtcag atgttaatga gtgatagttg taaaatcgcc attgccgcgg
     7261 gaattgacga tccacaaaat cccattggta ccgatgccgt caaagtgatg gaggccatcg
     7321 aatctgttgc tgatgccgac catgtgctgg tcatgatgga tatgggtagc gcattattga
     7381 gtgctgaaac tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta cgtttgtgtg
     7441 ctgcgccgtt ggtcgaaggt acactggcag caacggtcag cgcggcctcg ggggcggata
     7501 tcgacaaagt tatctttgac gctatgcatg cgctggaagc caaacgtgaa caactgggtt
     7561 taccgtcctc cgacactgaa atctctgaca catgtcctcc gtacgatgaa gaagcccgtt
     7621 ctctggcggt ggtcataaaa aaccgtaacg gcctgcatgt acgtccggcc tcccggctgg
     7681 tttatacctt atcgacattt aatgccgata tgttgctgga aaaaaacggc aaatgcgtca
     7741 caccagagag tattaaccag attgcgttac tacaagttcg ctataacgat acgctgcgcc
     7801 tgattgcgaa agggccagaa gctgaagagg cactgatcgc tttccgtcag ctggctgaag
     7861 ataactttgg tgaaacggag gaagtcgctc cacctactct gcgtcccgtt ctgcctgttt
     7921 cgggtaaagc cttttattat caaccagttt tatgtacggt acaggcaaaa tcaaccctga
     7981 ccgcggaaga agaacaagat cgattacgcc aggctattga cttcacgtta ttagatctga
     8041 tgacgttaac agcgaaagca gaagccagcg ggcttgacga tattgccgca atcttttctg
     8101 gtcaccata
//
LOCUS       DAJTNM010000155         7933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTNM010000155 DAJTNM010000000
VERSION     DAJTNM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.155"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..1242)
                     /locus_tag="OO172_003657"
     CDS             complement(<1..1242)
                     /locus_tag="OO172_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5513672.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRNAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSL"
     gene            complement(1239..2117)
                     /locus_tag="OO172_003658"
     CDS             complement(1239..2117)
                     /locus_tag="OO172_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5513673.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2128..3035)
                     /locus_tag="OO172_003659"
                     /pseudo
     CDS             complement(2128..3035)
                     /locus_tag="OO172_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3022..3255)
                     /locus_tag="OO172_003660"
     CDS             complement(3022..3255)
                     /locus_tag="OO172_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513674.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3252..4109)
                     /locus_tag="OO172_003661"
     CDS             complement(3252..4109)
                     /locus_tag="OO172_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5513675.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4134..4463
                     /locus_tag="OO172_003662"
     CDS             4134..4463
                     /locus_tag="OO172_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5513676.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4453..4761
                     /locus_tag="OO172_003663"
     CDS             4453..4761
                     /locus_tag="OO172_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5513677.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4803..4979)
                     /locus_tag="OO172_003664"
     CDS             complement(4803..4979)
                     /locus_tag="OO172_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR5513678.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4976..5386)
                     /locus_tag="OO172_003665"
     CDS             complement(4976..5386)
                     /locus_tag="OO172_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR5513679.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5383..5661)
                     /locus_tag="OO172_003666"
     CDS             complement(5383..5661)
                     /locus_tag="OO172_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513680.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5736..>7933)
                     /locus_tag="OO172_003667"
     CDS             complement(5736..>7933)
                     /locus_tag="OO172_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HCR5513681.1"
                     /translation="AKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASRLGEHR
                     CRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQ
                     YLFNPPWESLADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQ
                     VASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVID
                     TFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEP
                     YMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFF
                     SANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTG
                     VTYTYKDDKRHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIG
                     NAECLCIMVDHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLK
                     RLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLF
                     IIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVK
                     GSGAITDLTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGE
                     GWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         1883 a         2147 c         1823 g         2080 t
ORIGIN      
        1 cagagaaccg ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc
       61 cgggttgtac acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc
      121 tccggaattt ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg
      181 ggagagctgg ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc
      241 ctttgagatt tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc
      301 gagataatca accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt
      361 tgcgcatatc tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt
      421 catccgcccc accccttgtg agaaacgcgt ccagtcttca tcttcaaagt gagccacaga
      481 tttcaggcgt gatactggca tccctccagc cgcagacacc atacgttcac caatctggat
      541 gttcgccatc tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat
      601 gatgtccagc gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc
      661 tccgggctcc ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct
      721 ggatttctcc agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc
      781 atcaatatgt accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg
      841 cgcatccttc agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag
      901 cgcctcattc agtgtttttt ctgcattgcg cagtgcggca ttgcgccgca acgctgcaac
      961 atagtgctcc agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc
     1021 aagttccggc atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg
     1081 tctgcagatg cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag
     1141 tgtggccaga atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac
     1201 agcgccttca aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata
     1261 cgagaacggt aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg
     1321 tcggcaattc ggggactgat gaacgcccac aactcttccg gtgagaggtt actgatcaga
     1381 atggtgggca agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca
     1441 gccgcgctgc caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa
     1501 cgctcaataa cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca
     1561 cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt
     1621 tttgccatag ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga
     1681 ctggtaccgt tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga
     1741 tttctggctg cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc
     1801 agggcaattc cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc
     1861 aaatcgttgg cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg
     1921 ccattacgat cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat
     1981 ccgcgacctt cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca
     2041 aatgcaatct ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc
     2101 aggttaaacg tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa
     2161 tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc
     2221 gggagcggct ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc
     2281 agcctgcttc acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg
     2341 tttcacgcca tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc
     2401 tttgaaggct gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg
     2461 ttcaaattcc ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcaga
     2521 tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc
     2581 tgtaagatcg aaattggttt tcccttctcc gcggcgaggg gtttcccgtg tccgcggtga
     2641 aggctttccc tcctccgcga aattgggttt tacagtttcc cgaaaacggg tttccccatt
     2701 tcgggaaact gattgttttc attgataatt tcattaaggc gctcacaatc tatacggtag
     2761 aacattttgt gctcaagacg cttgttggtt tcaaccaaaa tgcctctgga cacaagatgc
     2821 ttacgcgcta cagcctgttg ttcaaatgta agtccggttt cgtgttgtat ctcttcacgc
     2881 gttttatgta cgccttccgc tgcatgtgct ttatcctacc agtaaaaaat ctgaccaaag
     2941 aaaataacag cgtgcggact tcccatgtat ttaacgagcc cagggtaata agcaaccgga
     3001 tgtccaaaat cgagcagaag atcagacgga cgcatagcca cctcccaggc gtttaaacat
     3061 ttttccgggc tgaaacgcca ccagcggata actcagggta tgagtacgtc cctgaacctg
     3121 gcagacaacc ttctggcttt cggtactgac caggcaaacc cgcagaacgt agccgttgct
     3181 ggtggtgaac cactgcccca cacgggggca atggttgtat cggtgataca ggaaattaac
     3241 gatgtggcgg atcatgggag cacctccttg tcagaaccat tcagcctgga atcaacaagt
     3301 gcagcaccaa aaacagcatc acctacacgg tcgtacagtt tgccagccag cggagattca
     3361 acggccttaa gcattgggta aagctggctt gtccagattt gatggatttc acgcaaatgc
     3421 aggtatacgc ctctggcgtt ttgtgcgaca gatgacatgt cagccgcacc agctcctgat
     3481 aagctcctct ccatctggtt aaaggcattg atgtatgcct ctttgaaccg ggcggcacgt
     3541 ttaccagtga agcccatagc aaggaaggcg aagccgtcgc gggtaatttg gtagcaggga
     3601 agtttgcgag taccgccgtt gggctggcgt accaaaattg atgtctccgc aaaattgcgg
     3661 gcacaaaact ctggagaaca atccaaaatg cggatctttt tcagaacatc gtcatgacgt
     3721 ttagagaaga agttggcaac agccagggat gaagtaacag cctgaccatc aacgatggca
     3781 atttcaggtt gagagagggt tgggagagta gtcatggtga cagcccctat gttgaattca
     3841 aagaactcac cacatgggac gccaatcaca gaggtggtga gacgtacagg gttggcgtaa
     3901 ccggtcaaca tagaacccgg cgcatcttgc gatgcccctg cacgccccac cataatttgg
     3961 gcgtagcaat gctcatgaca cgaaaaaacc gcatgagcgc ggttatgctc tatattgaat
     4021 ttcaggacgc caatcccggc acccgcttta taaggtgcag agacagtgta acgtcccgaa
     4081 attgcagaat caatatattg tgcagatata acgattttgt tatgttttgc tgtatgaact
     4141 acactatcga atactacagt gaagaggtcc gactggaggt cgatcagctt ccattgagta
     4201 tgcgtgccag ataccagcat cttgttgaac gcatgaaggt atacggcagc aatctcggag
     4261 aacctcacac cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg
     4321 ggatcgcgcg tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt
     4381 ttgtaaagaa aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga
     4441 aggaggttaa gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca
     4501 gccaaatgga tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc
     4561 gcactgcttg atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt
     4621 gccgagcgaa tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca
     4681 ggtatcagcg gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa
     4741 ctccagatcc gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat
     4801 agtcatttcc tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg
     4861 aaggttttta cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt
     4921 ggaaggtatt tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatt
     4981 catacctcct gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt gtgtactcga
     5041 tcagacttgc catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt
     5101 ctccttcaag gccgggcaaa acatcagctt cctgttttgt tgccactgca tgaccgctga
     5161 tcaacaaaac cttccattgt tctggtttta accatttgcc gcaccattga acctgacgag
     5221 cgatatccgc cagcatcgcg tgaaattttg cgttctggtc aagatttcgc ttgtagtcag
     5281 taatgcggat ggtgactggc ttgtctttat cgagaggagt tgagaggatg gcatttattg
     5341 cggcttgctg ttgttgctta gttcggagga agattgtttg cttcatcgaa aattcttctc
     5401 tttaattcca gcggctctga tagctttcat tactgcaatt accgttttgt ccttcccatc
     5461 ctcatgcccc atcgcataag caccttcttc accatctttc caaaagtcgt cattcgattc
     5521 gggccagtcg atatccagtt caatagctgc tcgcggtgcc tgccatatca cccaggcaaa
     5581 ctcttttaat tcatcgtctc ctgtgaactg gcttttgtct tttgaccacc agttttcaaa
     5641 ctgtcggtag ctatcgttca cttccctctc ccccaaataa aaaggcctgc gattaccagc
     5701 aggcctgcta ttagctcagt gatgtagatg gtcatttaat actccgtcac gttttcctgt
     5761 cgccacgcct cgtcatattc cgatttcggc atattggcga tgtagctata tggcgatcct
     5821 gattcaagtt gcaggaactg gtgcgattgc tcgtcaagga acaacgggac accaccttcc
     5881 caaccttcgc cgttacgttg tttttcaagc atcaaaacag atgccggaga tgccagtagc
     5941 tgttcgtcct tctctgacat cttttcacca ctctgaactc tctgtaacgc tctctcgcga
     6001 gccttgttac gccagatgat gaaaaggttg tctgtcaggt ctgttatcgc tccagagcct
     6061 tttacgtcca ttttcccggt tggtttttct tcgctgtctc cttttcgcga gtgagtaacg
     6121 agaatgacgt gggagtttgt tttgtttttg aagtcgcaaa tcgagtcaac aaacgccttc
     6181 tgcccgttat agtcatcgtc gcctatgcca catttcatca ggctgtcgat gatgaataac
     6241 tggatcccgt atcggcggcg agcgtagtcg aatatttcga tcagcctgtc ggctttcgcc
     6301 gttccagtca ggccaaacac ccaaagtctt tcgtcataaa atttaaatgc agagtcaatt
     6361 tccagcactg gcggcatctt gcagcacgtt gcctgacggg taagtcgctt aaggagaata
     6421 ccaggcttca gctcaagtga cgcgatgcac gtcttcacac cctgacgcat tgcctcaagt
     6481 gccatatgcc cgacaacctc ggtgttatgc gtcgccacat atcccctggt tacatacagg
     6541 tggcgagggt gatcgaccat aatacacaag cattctgcat tgccgatttt ttcaacattc
     6601 ctgacgaata ccccacagcc ctcgaatcga tgcgcagtta atctgccatt gagtcgtggt
     6661 gaacggatgg cctccctgat ttctcttgtc agtctgattc ttgcctcgta tgaatccatc
     6721 ccatgccgct tatcgtcctt gtatgtgtat gtcacgccag ttttaactcg cgtccggcat
     6781 gacccgccga gtgagttaac cagttgaaca accccattgc gcagttcttc acttgctgag
     6841 gaaaaaacaa gcgttccatc cttctcgaca tacccatccg tttcaagcag accacacagc
     6901 atgcctatac gcgttgattt atttgcggaa aaaaacacac gagggatgaa tttgtttttt
     6961 gctgtgcacc ccattagtcc ataacctcgc agggtctcca tgagtggatt tacctgacca
     7021 cgcgccgttg atatcagcca gtccttacca tctccagaga agttgtaatc aggcagttca
     7081 gccttcatac gctcgatcat gtatggctcg acgtttgaaa acttcacgct cccattgcta
     7141 agactaccat ccccgagaag ggagccgata acccatgcta atggctctga gtggtcgcca
     7201 aagtcaccag ttatttcagg aatcctaacg ccatttttgt gcctcttcgt ttcactcaac
     7261 cgcttcaacc cgaaggtatc tatcacgcgg cgtttctcgc ctttcgtgaa tccacgacta
     7321 gtgacctccc ataggtgatc gcctgcgcaa tcaacataac gaccatcttc aaatgtgact
     7381 cggtaaacat ctctaacacc ctgcgggaat atcccagtga cagttgacgg attgccgtct
     7441 actgacgcca cctgatcgcc aatttttaca ttcccgtgag tagtccatgt cccatcagct
     7501 aaaagaatag gctcattcag tgggcatgcc tttccgtgac cgttcacacc attgaccagc
     7561 gtcaactctg cctcacggaa ctggaattta tctgccagag attcccacgg tggattaaac
     7621 agatactgct gcttgccgta gaaagcgttg atagtgtcct ggtaaaactc tcgcgcactg
     7681 tagagttctt caggatcgaa gtaggatgcc gtgccgatgt actgccagat ttcatcctcg
     7741 gtaacaccgt tcatcaggca ttcgttgatg tctttgtacg gcagagtaac aagacggcaa
     7801 cgatgttcac cgagtcggct tgcgatttcc cttgcggctt cacgaccaac atcatcaacg
     7861 tccatcgaga tgaatatttc ctcaaacctg tcgaggttat gatactcaaa ctcaatccac
     7921 tgttgcttag cgc
//
LOCUS       DAJTNM010000156         7852 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTNM010000156 DAJTNM010000000
VERSION     DAJTNM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.156"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="OO172_003668"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="OO172_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5513682.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /gene="dcuS"
                     /locus_tag="OO172_003669"
     CDS             323..1954
                     /gene="dcuS"
                     /locus_tag="OO172_003669"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5513683.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="OO172_003670"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="OO172_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5513684.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="OO172_003671"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="OO172_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5513685.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="OO172_003672"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="OO172_003672"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5513686.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAVIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6422..7051
                     /locus_tag="OO172_003673"
     CDS             6422..7051
                     /locus_tag="OO172_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5513687.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7190..>7852)
                     /locus_tag="OO172_003674"
     CDS             complement(7190..>7852)
                     /locus_tag="OO172_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5513688.1"
                     /translation="AIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRI
                     LWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDY
                     GEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFI
                     MIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDV
                     KA"
BASE COUNT         2030 a         2004 c         1930 g         1888 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgacttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc taggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtaacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctccgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc ggtcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaccaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaattgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc acctggcagc atgctgccag gtgctgggcc acctcttttg
     6361 cgattgtaat ttcatgcttg ctggtgaata gtcagtattt tccctgactt gcgaactcac
     6421 catgacccgc acactcaagc cgttaattct taacaccggc gcactggcgc taacgttaat
     6481 cctgatttat accggcattt cggcccatga caaactcacc tggctgctgg aagtgacacc
     6541 ggtgattatc gtcgtgccgc tactacttgc caccgccaga cgttacccgt taacgctgct
     6601 gctctatacg ctcatcttct ttcacgccat catcctgatg gtaggcgggc agtacaccta
     6661 cgcgaaagtc cccgttggtt ttgaggtgca ggaatggctg gggttgagtc gtaatccgta
     6721 tgacaagctg gggcactttt tccaggggct ggtgcctgca ctggtggcac gagaaattct
     6781 cgttcgcggg atgtacgtgc gcggacgtaa aatggtggcg tttctggtct gttgcgtggc
     6841 gctggcgata agcgcgatgt atgaattaat cgagtggtgg gcggcactgg cgatgggcca
     6901 gggagcggat gattttctcg gtacccaggg cgaccagtgg gacacgcaat ccgatatgtt
     6961 ctgcgcgctg cttggcgcat taacgacagt gatattgctc gctcgctttc attgccgcca
     7021 gctacggcgc tatggcttga taaccggata acaacgtgca ctttgttgat gccggatgcg
     7081 gcgtgaattc cttatccggt ctacaaaatt tgcaaatcca aagcattaca ttaagacgtg
     7141 gcctgataaa catatcgcat caggccattt tcgttagcta ttgtccacgt caggctttca
     7201 catcactcac tgcaccgacc ggaatatcag catgaacagg ctcgggcggt actttgcgat
     7261 atttatccag cagatgaatc tggatcctgc gcagcgtgtc gccattgagg cgatagaaac
     7321 ggaagtagag aatcagcgtt accatgaaaa acagagtcgg tagagcaatc ataataaact
     7381 gcatacctaa tagggcttgc gtagactgtt caacattcgg tacatagcca atcatcccta
     7441 acacaaccgc aatgaaaaaa gccgcaaagg ctgaaccgcc cttcaccacc atagtctgca
     7501 cggagtaagc gatgctttca cagcgtacgt gcagtttata ttcaccgtaa tcaacggtat
     7561 ctgccaccat gatgacctgt aatacccaga aaagcgccgt tcccacattc agcaaaatac
     7621 ccgcaatcac aatgaggacg acgttgtgat agctcattaa tgccattaac aggagaacac
     7681 cacagcttaa caccggaaga atagatgctc cggcccataa aatgcgtcgg gataatgatt
     7741 taactaagcg tgggaagaat actaacgtca ccaggttagc agctcccgca tacgacagat
     7801 aataggggaa caaatccgca tcaccgataa catatgagaa ataatagata gc
//
LOCUS       DAJTNM010000157         7627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTNM010000157 DAJTNM010000000
VERSION     DAJTNM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.157"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..105)
                     /locus_tag="OO172_003675"
     CDS             complement(<1..105)
                     /locus_tag="OO172_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="represses the celABCDF-ydjC operon involved in
                     carbon uptake; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR5513689.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDS"
     gene            complement(113..463)
                     /gene="chbA"
                     /locus_tag="OO172_003676"
                     /pseudo
     CDS             complement(113..463)
                     /gene="chbA"
                     /locus_tag="OO172_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
     gene            complement(522..1870)
                     /gene="chbC"
                     /locus_tag="OO172_003677"
                     /pseudo
     CDS             complement(522..1870)
                     /gene="chbC"
                     /locus_tag="OO172_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(1955..2275)
                     /gene="chbB"
                     /locus_tag="OO172_003678"
     CDS             complement(1955..2275)
                     /gene="chbB"
                     /locus_tag="OO172_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5513690.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2574..2912)
                     /gene="osmE"
                     /locus_tag="OO172_003679"
     CDS             complement(2574..2912)
                     /gene="osmE"
                     /locus_tag="OO172_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5513691.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3114..3941
                     /gene="nadE"
                     /locus_tag="OO172_003680"
     CDS             3114..3941
                     /gene="nadE"
                     /locus_tag="OO172_003680"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5513692.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINLLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4171..5058
                     /gene="cho"
                     /locus_tag="OO172_003681"
     CDS             4171..5058
                     /gene="cho"
                     /locus_tag="OO172_003681"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5513693.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5018..5593)
                     /gene="ves"
                     /locus_tag="OO172_003682"
     CDS             complement(5018..5593)
                     /gene="ves"
                     /locus_tag="OO172_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5513694.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREISTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(5796..6281)
                     /gene="spy"
                     /locus_tag="OO172_003683"
     CDS             complement(5796..6281)
                     /gene="spy"
                     /locus_tag="OO172_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5513695.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(6611..7579)
                     /gene="astE"
                     /locus_tag="OO172_003684"
     CDS             complement(6611..7579)
                     /gene="astE"
                     /locus_tag="OO172_003684"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5513696.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         1981 a         1915 c         1789 g         1942 t
ORIGIN      
        1 actatcagtt ttgttataga taaacacatg gaagtttttg ccattaaaca actgctgttc
       61 tcgggcagtg gcaatttccg gcgcgttaat cactggctgc atcatcgact ccttatgcct
      121 tcagtttttc atgaagctca attaattcag taatcagttc acgcgcaagc atggacgtca
      181 ttaaatgatc ctgagcgtgg accagcacca gactcacttt catcttacct tcgcccgcat
      241 cgccttcaat cagtttcgtc tgtaccagat gcgcttcatt caatgccatc cgtgactgat
      301 ccatcatggc tttcgctgcg gcaaaatcgc cctgcttcgc ctgtttcaat gcggcatacg
      361 ccaggctacg cgcttgtcca gagttgatga taagccccat caccacttct tacagctctt
      421 cagcttctgt ttgcgtatca ggaatattat cgagatccat catacatcgt tcctctctct
      481 tcttaccggc aagattaccc gtaccggcat cgattaaaat ttcaggggtt agcgatatct
      541 tcttcgctct cttctttatc aatcgcattt tgtgctttgt tagccaccac aacaaagggc
      601 agataaatta acgttgcgat gccaaggttg aagagtgcga ccagcaatgc ggcgacgcta
      661 ccgttggtgt taaagaaggc tcccagaccg gttggcatgg tccacggtgc aatattggtc
      721 accggaggaa tgatgcccat gtagtacgct gcgagggtga ttgccgccag aatcggttgt
      781 accagtacaa acgggataaa catcaccggg ttcatgataa ttggcagacc aaacagaatc
      841 ggttcgttaa tctggaagat gccggacggc agcgccagtt ttgccacctg acgataatca
      901 gcacggcgag aggcgataaa gatagccagg atcaggccta aagtcgcacc actgccccca
      961 aggaaaataa aggagtccag catcggcttg gcccagatat ggaaggtctt accggctgcc
     1021 agcgccgctt cgacggaacc atattgctga taggtcgcga tattttccag cgcccacggc
     1081 gtcataatgc cgttgtccag tgcggtcagc gccagcgcgc catgaatacc gaagaaccag
     1141 agcagtggaa caaagatcac ataggcccag cccaccacgc tacccaacga tgccagtggg
     1201 gttgagatgg tatccataat gatctgatgg aagttggtgc cccaggtatt caacgcccag
     1261 gcaataatcc ccatcacgga aagaataata aagccgggaa ttaatgccga gaaagaacgc
     1321 gatactgaag caggtacgct gtcgggcagt ttaatgaccc aattgcggcg gacaataaag
     1381 gtaaacattt ctgccaccac caggccaata ataatcccgg agatgatatt cgccccacct
     1441 aaccagtttg cgccaaccgc ataggcctca ccgacactat atggggtgac ggtcataaat
     1501 gctgcaacgg ataacaaccc agccgccagc gcatcgactt tacgctcttc tgccagcgcc
     1561 atgccaataa agaacggtgc cattaaagac attattccta atgttccgtt atatacgttg
     1621 ccgccaatac ctttcagacc attaagtgtt tcaatggttg aggcgtcgag gcgaatacct
     1681 aaggaataaa aaacgacccc tccccaaagc ttagaaaaac gttgttaatt aatacaaaca
     1741 tggcccccgc aagggttaac ggcattaagc gaataaagcc atttttgatt gcattaacgt
     1801 gtggctgctt tcctatttta actgcaaaag ggaggagtac cttttcaagc gatgcaataa
     1861 cattactcat agaaaaatac ccttaaaaac cgcaatttaa atattgcggt attgatttat
     1921 gaaataactc tttgacggga aaatttaaaa taaattaatt tgctgcggct tttttaatcg
     1981 ctgcaaccgc agccttaagc acgcctaaac catcgacttt gccataaagc agcgagtcaa
     2041 ttacttcaac cggtttgttg ggtaacaaac gctggatttc gggcaacata taagcaatct
     2101 gcggccctaa taacacgaca tcggcattct gacctttttc accagccagt gtttccggaa
     2161 atgcttcaat aatgaccgga acttcatatt tttctgcctg tgcgcgcatt tttgatacca
     2221 gtaaagagat agacatgccc gcagaacaaa acagataaat gtgtttcttt tccataaaac
     2281 tgccctcatc gacgattatc tgtcagccag acactccgca agccttaacc tgcttccatg
     2341 ctctgggtaa cttgcgaaac caacatgact ttttttgtgg ctgaaaggag tatacggtat
     2401 agacctgagc aatagtattt ctttgacctt ataaattgtt tctccttgac ctgtggttat
     2461 gaccctcttc acatttcggg caaataattc gcgaagataa ttaagaactc aggccaaaaa
     2521 aaaccggcgc aatggccggt ttccgttgtt actcaagctt tcagacgaat tgattacttc
     2581 gcagcctgtg gatcagtgtc gtattcagca caggtctgat aaccggagtt gatgacatgt
     2641 ccggtatcat ctaacgcgac aaagtaggtt tctgctttac catcacgttg acccaggatg
     2701 taggtctggc aagtaccgcg agcatggatc atgctcactt cagacgaagg tttacccgca
     2761 atttgtgcaa cctgcgcccg gctcatgcct tttttgacgt ctttcaccac aggctgtaca
     2821 aactggtctt tggtacgatc ataagctgta caacccgcca gcatggttaa taccgccgct
     2881 gcactcagaa ttcctgccat attcttgttc atattccgtc ctcttgttta tcagcgtgtt
     2941 agataagcct ggaatacatt gggcgctttt tcaagcccgt gaacgaaacg gctccgcttt
     3001 cagaggattc ctgtatgacg ttttaaccac cattcagccc gctgtcgctt gtcgtttcag
     3061 tagcaacggg ttagctttaa ggaagttttg tcttttctgt ctggaggggt tcaatgacat
     3121 tgcaacaaca aataataaag gcgctgggcg caaaaccgca gattaatgct gaagaggaaa
     3181 ttcgtcgtag tgtcgatttt ctgaaaagct acctgcaaac ttatccgttc attaaatcac
     3241 tggtgctcgg gatcagcggc ggtcaggact ccacgcttgc cggaaagctg tgccagatgg
     3301 cgattaatga gctgcgccag gaaactggca acgaatcact gcaatttatt gccgtacgcc
     3361 tgccctatgg tgttcaggct gacgaacaag attgccagga tgccattgcc tttattcaac
     3421 cggatcgcgt attaaccgtt aatatcaagg gcgcggtatt ggctagcgag caggcattgc
     3481 gggaagcagg cattgaactg agcgattttg tccgtggcaa tgaaaaagcg cgtgagcgga
     3541 tgaaagcaca atatagcatt gcgggtatga ccagcggtgt cgtggtgggc accgatcatg
     3601 cagcagaagc cattaccgga ttcttcacta aatatggtga cggcggtacg gatattaatc
     3661 tgctgtatcg tctcaacaaa cgccagggta aacagttact gacggcatta ggttgcccgg
     3721 aacaccttta taagaaagcc ccaactgccg atcttgaaga tgatcgccct tctctgccag
     3781 atgaagtggc actcggcgtg acctatgaca atatcgacga ctatctggaa gggaaaaacc
     3841 tacctgaaca ggtcgccaga acaatagaga actggtatct gaaaaccgaa cataaacgcc
     3901 gtccgccaat taacgttttc gatgatttct ggaaaaagta ataatttgca cctattggat
     3961 tgtgcgaaaa agagtaattt gttcacgccg gatgcggcgt gaacgcctta ttcgacctac
     4021 aaaactatgc aaattcaata cattgcagga gtcgaatagg cctgacaggc gtagcacgtc
     4081 agacggtgta acctttgtca tcaacccgcc tcttttttaa tcgcctcccg cctgttacac
     4141 tggatagata accagcattc ggagtcaaca gtggtacggc gtttaacttc tccgcggctc
     4201 gaatttgaag ctgcggcaat ttatgaatat cccgaacatt tacattcatt ccttaatgac
     4261 ttacccaccc gaccaggggt gtatctgttt catggcgaaa gtgacaccat gccgctctat
     4321 atcggcaaaa gcgttaatat ccgcagtcgg gtgctttctc atttacgcac cccggatgaa
     4381 gccgccatgc tacggcaatc ccggcgaatt agctggatct gcaccgcagg cgaaatcggc
     4441 gctctgctcc ttgaagcgcg attaatcaaa gaacaacagc cgctgtttaa taaacggttg
     4501 cgccgcaatc gccagctctg tgccctgcaa ttaaatgaaa agcgcgtcga tgtggtgtat
     4561 gccaaagagg tggatttttc acgagccccc aacctgtttg gcctgtttgc caataggcgc
     4621 gcagctttgc aagcattgca gagcatcgct gatgaacaaa aactttgtta tggcctgttg
     4681 ggactggaac cgttaagtcg cggtcgtgca tgttttcgtt cagcgctaaa acgttgcgcc
     4741 ggagcatgct gcggtaaaga gagccatgag gaacatgcgc tacgcttgcg ccagtctctg
     4801 gagcgtttgc gggtggtgtg ttggccttgg caaggtgcgg tggcactgaa agaacagcac
     4861 ccggaaatga ctcaatatca tattattcaa aactggctgt ggctgggggc ggttaattcg
     4921 ctggaagaag cgacaacgtt aattcggaca cccgccgggt ttgatcacga cggttataaa
     4981 attctttgta agccgctgct ttccggtaac tatgaaatta ctgaacttga tccggcgaat
     5041 gaccagcgag ccagttgatt tcactgaaca acagtttacc ttgcggttgt aaaagccgca
     5101 gggtgtgcct gccatcgaac cagcacgcgc cctggtcggt agtgagcagt ttgtcgccca
     5161 gttgccacgc cccgttaatc acaaacacca ccccgccgcg tgagccaaag gtagtaaacg
     5221 tacgttcggc aattctgact ttcgccttac agacatccag gcgcgtcata atattgaaat
     5281 ccatcgacat ctgccctgcc gtcagtttcg cttttaccac ctggtccgcc gtaaaggcaa
     5341 aaggctgtaa cggttttaag gtatggttaa agcggtctgc gctttcaagg agcatctcgc
     5401 cgccttccag caacgtcact atcctttcca tgccgggaaa caaagaaaac tccccattcg
     5461 ccgcgatgga tgcaatgctg gcacgccagt aaaaatcacg ttttgccggt gggaaagtgg
     5521 aaatttcgcg cgtttcaccg gcagcgtttc gccacagatt caccgacatt ttacgcatat
     5581 caaagtattc catactcgct cccggcccgt tcttttcatt gttatcgttc aggtaaccgt
     5641 ggatggttat cggcaactcg ggtgatttac gttagtggtg atcaggcttt ttttacaact
     5701 gtttgagaag agaaaagaaa accgccgatc ctgtccaccg cattactgca aggtagtgga
     5761 caagaccggc ggtcttaagt tttttggctg aaagattatt cagcagttgc aggcatttta
     5821 ccttttgccg ctggacgttc tgtcagacgc ttctcaaaat tagcattaaa ttgctttttc
     5881 tgttccggcg tcaggatgtt gtaaattttg ttctgggttt ccatgtgcgc cagcatgtta
     5941 gctttgcgct gttcttccat ttttgcgatc tgcgcttcag cttttgcttt atcgaaggta
     6001 tcgctggcaa tgatgtcatg cattgcgcgg cgttcttcca gcggcggacg tttcatctgg
     6061 tcacgctggc ctttcatgat ttcgtggatc tgctgtttct gcgcgtcggt caggttcagg
     6121 tctttgaaca tcatgtcctg atgcggaccg aacttgcctt tgtggtgcat catcggcttc
     6181 gcgtcagccg gtgctgcggt agtggtgtct gcggcatggg ccaggttagc cgcgccaaga
     6241 gccagggtag aggcaacaaa cagtgcagtt aatttacgca tattctatat ccttcctttc
     6301 agttatttat tacggctttc ttaagtagcg tgccgtgttg acgagattaa ctttactgac
     6361 tttagcgtca attaatcaga gcaacggtaa aacaatgaaa gtgtaaaaaa cacttttgcg
     6421 ccaattatgg agaaaaaaag aaaatttgat ggagagtgat gagagaatat tacaaaacga
     6481 tgattttgca gagattatga agaactatac cggatgactg gtgataaata aagcaaataa
     6541 ccaggattaa tctgtattaa tttataagaa agcaacttaa tacccgcaga atgatttctg
     6601 cgggtaagta ttagcttatt ttttcgagca ttaatcccgc gcgtaatccc aacgctacca
     6661 acggattagg gaataacaca tactctacat catgggttac ggtaaaacgt tcctctccgt
     6721 cctgcgccag caatgttcct ttctcaaacg gcataaaatt cagcgtgtca cttgccatat
     6781 gcatttcgaa ggacggcgag tggcgagtaa tttgcgaaac cacccgataa cggagcggcg
     6841 gtgttctcac gataccgaca ctctcaccag atagcagcgc agcaattgcg ctggcagtta
     6901 ctgcaaactg gcgaagatcg ttttgcccaa agggcaacgc tttgccaagt tccaacgtac
     6961 aggccagcgc gccaaaatgt ctgacgctga aatgggtaaa cgtaccacca ggctcctgat
     7021 ggaacaccag cgcctccagc cccgccgcac ccagccacgt cagaaatttc tcgtcccagg
     7081 gaatgtcgcg ttgcggtaat acaccgaact gcggatgcaa ggagccacga attgcggtat
     7141 gtagatcaag gtgccaacgc acagattctt tgccctggtc ataaaaatct tccaggcact
     7201 gttccagttc gcgcgcccga caggtttctc cgctttcagc aaatagctgc caacgaccgc
     7261 caaacattcg gttcatatcg ctatggcaat aacgtttccc ttgcttcagc gcaggaggat
     7321 tcccgaggat caccagcaac cgccaacgta acgggatctc gctgtgagat atcgcgccaa
     7381 gcaacgcgtc cagcatttcc acaggtgccg tctcattacc gtggatcccc gcagaaatca
     7441 ccagcgcgcc ttgcggtggc gttaatggcg tcagttccag cacaccatcg cccagccagc
     7501 gccagcgaac gccgttgatt tctcgctcgg tgataaccgg ttttttaccc gttaaggtca
     7561 gagcaagaaa attatccatt gccccctccc tcgcgctgga acggataaac cgaaccgaga
     7621 ttcagta
//
LOCUS       DAJTNM010000158         7508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTNM010000158 DAJTNM010000000
VERSION     DAJTNM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.158"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="OO172_003685"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="OO172_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCR5513697.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDDLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLCGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            1158..2117
                     /gene="rbsC"
                     /locus_tag="OO172_003686"
     CDS             1158..2117
                     /gene="rbsC"
                     /locus_tag="OO172_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5513698.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIVGT
                     LIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2142..3019
                     /gene="rbsB"
                     /locus_tag="OO172_003687"
                     /pseudo
     CDS             2142..3019
                     /gene="rbsB"
                     /locus_tag="OO172_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3145..4074
                     /gene="rbsK"
                     /locus_tag="OO172_003688"
     CDS             3145..4074
                     /gene="rbsK"
                     /locus_tag="OO172_003688"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5513699.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQLLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4078..5070
                     /gene="rbsR"
                     /locus_tag="OO172_003689"
     CDS             4078..5070
                     /gene="rbsR"
                     /locus_tag="OO172_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5513700.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5036..6463)
                     /gene="mdtD"
                     /locus_tag="OO172_003690"
     CDS             complement(5036..6463)
                     /gene="mdtD"
                     /locus_tag="OO172_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5513701.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVTRLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6486..7178)
                     /locus_tag="OO172_003691"
     CDS             complement(6486..7178)
                     /locus_tag="OO172_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5513702.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1909 a         1816 c         1979 g         1804 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatgacc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctctgcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcatcgtc tattctcttt gtggtctgct ggcatcgctg gccgggatca
     1861 ttgaagtggc gcgtctctcc tctgcacaac ccacggcggg gactggctat gagctggatg
     1921 ctattgctgc ggtggttctg ggcggtacga gtctggcggg cggaaaaggt cgcattgttg
     1981 ggacgttgat cggcgcatta attcttggct tccttaataa tggattgaat ttgttaggtg
     2041 tttcctccta ttaccagatg atcgtcaaag cggtggtgat tttgctggcg gtgctggtag
     2101 acaacaaaaa gcagtaataa cgactacagg acatcttgaa tatgaacatg aaaaaactgg
     2161 ctaccctggt ttccgctgtt gcgctaagcg ccaccgtcag tgcgaatgcg atggcaaaag
     2221 acaccatcgc gctggtggtc tccacgctta acaacccgtt ctttgtatcg ctgaaagatg
     2281 gcgcgcagaa agaggcggat aaacttggct ataacctggt ggtgctggac tcccagaaca
     2341 acccggcgaa agagctggcg aacgtgcagg acttaaccgt tcgcggcacc aaaattctgc
     2401 tgattaaccc gaccgactcc gacgcagtgg gtaatgctgt gaagatggct aaccaggcga
     2461 acattccggt tatcactctt gaccgccagg caacgaaagg tgaagtggtg agccacattg
     2521 catctgataa cgtactgggc ggcaaaatcg ccggtgatta catcgcgaag aaagcgggtg
     2581 aaggtgccaa agttatcgag ctgcaaggca ttgccggtac atccgcagcc cgtgaacgtg
     2641 gcgaaggctt ccagcaggcc gttgctgctc acaagtttaa tgttcttgcc agccagcaga
     2701 ttttgatcgc accaaaggtt tgaacgtaat gcagaacctg ttgaccgctc atccggatgt
     2761 tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcaaa ctgccggtaa
     2821 atcggatgtg atggtcgtcg gatttgacgg tacaccggat agcgaaaaag cggtgaatga
     2881 tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga aaggcgttga
     2941 aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatccgg ttgatctgaa
     3001 actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac ataaatacgt
     3061 catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc taacacggtg
     3121 gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc
     3181 attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga aaccgtaacc
     3241 ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggctgt ggctgctggg
     3301 cgtagcggtg cgaatatcgc gtttattgcc tgtactggcg atgacagcat tggcgagagc
     3361 gttcgccagc tgctcgccac tgataacatc gatattactc cggtcagcgt gatcaaaggc
     3421 gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt catcggtatt
     3481 catgccggcg ctaatgctgc tctttcccca tcgctggtgg aagcgcaacg tgagcgtatt
     3541 gccaacgcgt cagcgttatt aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg
     3601 gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc ggctcgcgaa
     3661 cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac ggaagcagaa
     3721 aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg
     3781 catgaaaagg gtatccgtac tgtactgatt actttaggaa gtcgtggtgt atgggctagc
     3841 gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga taccattgct
     3901 gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa accattgcca
     3961 gaggcgattc ggtttgccca tgctgccgct gcgattgccg taacacgtaa aggcgcacaa
     4021 ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag gtgacgcttg
     4081 gctacaatga aagatgttgc ccgtttggcg ggcgtttcta cctcaacagt ttctcacgtt
     4141 atcaataaag atcgcttcgt cagtgaagcg attaccgcca aagttgaagc ggcgattaaa
     4201 gaacttaatt acgcgccatc agctctggcg cgtagcctca aactcaatca aacacatacc
     4261 attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt gcgtggcgtt
     4321 gaacgcagct gcttcgaacg cggttatagt ctcgtccttt gcaataccga aggcgatgaa
     4381 cagcggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg cttactgtta
     4441 ctgtgtaccg aaacgcatca gccttcgcgt gaaatcatgc aacgttatcc gacagtgcct
     4501 accgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca ggataactcg
     4561 ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac ccgtatcgcc
     4621 tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg ttatcgggcg
     4681 gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg tgattttgaa
     4741 tttaacggcg ggtttgacgc catgcgccaa ctgttatcac atccgctgcg tcctcaggcc
     4801 gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata tcaggcagag
     4861 ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact ggcaagcttt
     4921 atgacgccac cattaaccac tatccaccaa ccgaaagatg aactggggga gctggcgatt
     4981 gatgtactca tccatcggat aacccagccg acccttcagc aacaacgatt acaacttact
     5041 ccgattctga tggaacgcgg ttcggcatag atttacgctg tcttttgatc aaattattac
     5101 catcggttgt tttcagaagc atgaacattg ctgctgaagc aacagtaata atgcccatcg
     5161 tgataaacgt atagtggaat tgttcgacag tcgttgtgcc ttccatccct tcataaatgc
     5221 gaaggacggc cgcacttaca gcaacgccta aactaatcga cagttgctgc gtgaccgcca
     5281 gaacattgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg atggtattca
     5341 tcgcggtaaa ctgcgtcgac atagccatcc ctaatataaa caacggcaag atcagcatcc
     5401 atatcgccat tgccggtgat tgcaaagaga actgagcgat cattagccca ataatcaccg
     5461 tgatccccac taatgtatgg cgatagccca gacgacgtaa gacttgggta accatcgatt
     5521 ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccggcaata aacgcctgat
     5581 aaccaaatcc tacctgtaac atcaatggca taaggaacgg tacacagccg gtccccagac
     5641 gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat aaatctaatg
     5701 aaattaatgg gtttggcgta cgtcgcgcat ggagaatata gagaagcagt aacccgatgc
     5761 tggtgacaat taccgtcaag gcaatccagc tggcgacaat cttttccccg aatagctcta
     5821 ttccgcttga aaagagaaca aggctgaggc caaacagcaa aaagccagtg atatcgaatc
     5881 tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac
     5941 ctatggggat attaattaaa aatatccagt gccaggttgc ccaggtgacc agcacgccgc
     6001 caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa ttcaataccg
     6061 gaagaagttc attacgagga taagcacgca gtaaggccag ccgagtaaca ggcatcatca
     6121 ttgcgtcgcc tatcccttga ataacccgga agacaaccag ctgtggtagc gaattagaaa
     6181 gtgcgcaggc cagagaaccc aacgtgaaca gactcacggc aagggtaaaa atgcgacgcg
     6241 taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc gtcagcgtat
     6301 aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga gcgattgcgg
     6361 gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaaaaaggcc atcgccgcga
     6421 tccacggcaa acccgacata ctacgcttct ttttatcgct cattcaatgt cctgttatcg
     6481 ggttatcact tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca
     6541 tcgctttgga taatcgcatc gacaatcgcc tggtgcagat ccagctttat cactgtgtcg
     6601 ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc gaatgaggtc
     6661 aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggctatatc gacttcgatc
     6721 cagcgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa cgtattgaga
     6781 tgcgccttct gttccgcggt gccaaccgtt gctgccagta ggcaggcttg cggctccaga
     6841 cagatacgca taaccagaaa gtgatcgatg acctgatgaa agttctcttc tgtcatccac
     6901 caagtaagca attcctgatc aagaaaattc cagttcgatt gtggcatgac ccgagtacca
     6961 attcgcggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc ttcgcgtacc
     7021 gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccgggcaa aatggtgccg
     7081 ggttcatatt caccttttaa gatccgttgc gccagcttct cagccagaac atacgaaagg
     7141 tttttctgtg cagctaactg ttgtgcgctt aaaggcatta cttatcttcc tttttctttt
     7201 tattcctcct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg gcaaattgat
     7261 tgttttatgg cacattaacg gggcttttgc tgaaaaaatg cgcggtcaga aaattatttt
     7321 aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca
     7381 acggcaaaca cgccgccggg tcagcgggat tctcctgaga attccgtcag agaaagcaaa
     7441 aataaatgct tgactctgta gcgggaaagc gtattatgca cacctcgcgc cgctgagaaa
     7501 aagcgaag
//
LOCUS       DAJTNM010000159         7460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTNM010000159 DAJTNM010000000
VERSION     DAJTNM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.159"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..233)
                     /locus_tag="OO172_003692"
     CDS             complement(<1..233)
                     /locus_tag="OO172_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR5513703.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAPIFP"
     gene            753..1448
                     /gene="minC"
                     /locus_tag="OO172_003693"
     CDS             753..1448
                     /gene="minC"
                     /locus_tag="OO172_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5513704.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1472..2284
                     /gene="minD"
                     /locus_tag="OO172_003694"
     CDS             1472..2284
                     /gene="minD"
                     /locus_tag="OO172_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5513705.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2288..2554
                     /gene="minE"
                     /locus_tag="OO172_003695"
     CDS             2288..2554
                     /gene="minE"
                     /locus_tag="OO172_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5513706.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2926..3270)
                     /locus_tag="OO172_003696"
                     /pseudo
     CDS             complement(2926..3270)
                     /locus_tag="OO172_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3305..3424)
                     /locus_tag="OO172_003697"
     CDS             complement(3305..3424)
                     /locus_tag="OO172_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5513707.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3385..3570)
                     /locus_tag="OO172_003698"
     CDS             complement(3385..3570)
                     /locus_tag="OO172_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513708.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3671..3844
                     /locus_tag="OO172_003699"
     CDS             3671..3844
                     /locus_tag="OO172_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513709.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3846..4190
                     /locus_tag="OO172_003700"
     CDS             3846..4190
                     /locus_tag="OO172_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5513710.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4194..4529
                     /locus_tag="OO172_003701"
     CDS             4194..4529
                     /locus_tag="OO172_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5513711.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4586..6907)
                     /locus_tag="OO172_003702"
     CDS             complement(4586..6907)
                     /locus_tag="OO172_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5513712.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPIPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7024..7233)
                     /locus_tag="OO172_003703"
     CDS             complement(7024..7233)
                     /locus_tag="OO172_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513713.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2047 a         1753 c         1655 g         2005 t
ORIGIN      
        1 tctggaaata taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga
       61 tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa
      121 acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag
      181 aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt catagcacat
      241 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
      301 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      361 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      421 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      481 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      541 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      601 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtgacttg
      661 cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg
      721 cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag
      781 gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc
      841 aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac
      901 tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg
      961 caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa
     1021 ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc
     1081 ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata
     1141 ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc
     1201 acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc
     1261 gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc
     1321 tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag
     1381 aattttatgg caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt
     1441 taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg
     1501 taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa
     1561 gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat
     1621 gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct
     1681 aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca
     1741 aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa
     1801 agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt
     1861 aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt
     1921 acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg
     1981 cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag
     2041 aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt
     2101 gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga
     2161 cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga
     2221 acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg
     2281 ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc
     2341 aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta
     2401 tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc
     2461 tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa
     2521 cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt
     2581 caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc
     2641 ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag
     2701 gctgttccca cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta
     2761 gtgtggatat gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg
     2821 atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat
     2881 cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt
     2941 cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat accccacccc
     3001 gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg
     3061 tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc
     3121 gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag
     3181 acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga
     3241 tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc
     3301 aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat
     3361 tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag
     3421 gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata
     3481 atactgtcgt acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt
     3541 aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc
     3601 agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata
     3661 ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat
     3721 tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt
     3781 taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat
     3841 ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc
     3901 cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt
     3961 tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg
     4021 gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg
     4081 ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag
     4141 gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga
     4201 tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg
     4261 actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg
     4321 aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca
     4381 gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag
     4441 aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg
     4501 acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga
     4561 cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg
     4621 tgtcattgta gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg
     4681 gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt
     4741 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     4801 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     4861 aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     4921 cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct
     4981 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt
     5041 aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat
     5101 cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt
     5161 ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac
     5221 tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac
     5281 cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc
     5341 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     5401 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta
     5461 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt
     5521 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg gggataggcg
     5581 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     5641 tagtcagata ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt
     5701 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     5761 tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa
     5821 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     5881 catccaattc agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg
     5941 ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat
     6001 caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa
     6061 gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg
     6121 aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg
     6181 atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt
     6241 catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta
     6301 tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg
     6361 tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt
     6421 ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat
     6481 gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac
     6541 cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga
     6601 tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg
     6661 cagtactttc ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg
     6721 caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa
     6781 aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc
     6841 cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac
     6901 cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc
     6961 ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa
     7021 tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat
     7081 aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag catgtttata
     7141 ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc
     7201 caaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     7261 caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa
     7321 aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag
     7381 tcggcattat catttttcac cttattttca tgacattgat cactttgggg tgactatttt
     7441 gtttaacaaa taactcttcg
//
LOCUS       DAJTNM010000160         7360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTNM010000160 DAJTNM010000000
VERSION     DAJTNM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.160"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            45..2225
                     /locus_tag="OO172_003704"
     CDS             45..2225
                     /locus_tag="OO172_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5513714.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2366..3649)
                     /locus_tag="OO172_003705"
     CDS             complement(2366..3649)
                     /locus_tag="OO172_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5513715.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3784..4854)
                     /locus_tag="OO172_003706"
     CDS             complement(3784..4854)
                     /locus_tag="OO172_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5513716.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(4832..5050)
                     /locus_tag="OO172_003707"
     CDS             complement(4832..5050)
                     /locus_tag="OO172_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5513717.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(5135..5758)
                     /locus_tag="OO172_003708"
     CDS             complement(5135..5758)
                     /locus_tag="OO172_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR5513718.1"
                     /translation="MTSQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            complement(6080..6787)
                     /locus_tag="OO172_003709"
     CDS             complement(6080..6787)
                     /locus_tag="OO172_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5513719.1"
                     /translation="MAQLYGTDVVRIRQNHHENKSRFVEGVHFYKVVGDELRALKHKV
                     ALNYFVKSAPNARHLILWTERGAARHAKMLETDQAWDVFEKLEDGYFNQREKVANHPQ
                     PVPQPESSLDRARAMEIALNVADRLVNMFPNLSSESKQAAVAGVFNPIVGRDVVPLPA
                     ISEHYYSATEVGQRYGISANKVGRIANTYMLKTEKYGKWFIDKSAHNDKQVETFRYNE
                     AGVHKIEELIEGERKAA"
     gene            complement(6735..7169)
                     /locus_tag="OO172_003710"
     CDS             complement(6735..7169)
                     /locus_tag="OO172_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5513720.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIGAVVRNGCCAHPAKPS"
     gene            complement(7242..>7360)
                     /locus_tag="OO172_003711"
     CDS             complement(7242..>7360)
                     /locus_tag="OO172_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5513721.1"
                     /translation="VAELEEALRDKQALLEASEKRIAELEAQTVTVKEVGDA"
BASE COUNT         1706 a         1832 c         1817 g         2005 t
ORIGIN      
        1 atctttctgc gcacttcacg gtgcgcagat atctggagca tttgatgatc aagttatctg
       61 aaaaaggcgt gtttctcgcc agtaataacg aaataattgc cgaagaacat ttcaccggcg
      121 aaattaaaaa agaagaagca aaaaaaggca ctattgcctg gtctattctc tcttctcata
      181 atacgtccgg aaatatggat aaacttaaaa ttaagtttga ttcattagcc tctcacgata
      241 ttacctttgt tggtattgta cagaccgcta aagcgtccgg aatggaacgt ttcccgctgc
      301 cgtatgtgct gaccaactgc cataactcac tctgcgccgt cggcggcacc attaacggtg
      361 atgaccatgt ttttggttta tcggcagctc agcgttatgg cggtattttt gtgcctccgc
      421 atattgcggt catccatcaa tatatgcgtg agatgatggc tggcgggggc aaaatgatcc
      481 tcgggtcaga cagccacacc cgttacggtg cattagggac aatggcagtc ggtgagggtg
      541 gcggtgagtt ggtaaaacag ctgcttaatg acacctggga tatcgactat ccgggcgtgg
      601 ttgcggtgca tctgaccgga aaacctgcgc cgtatgtggg gccacaggat gtggcgctgg
      661 ctatcattgg cgcggtgttc aaaaacggtt acgtcaaaaa caaagtcatg gagttcgttg
      721 gaccgggcgt tagcgcgctc tctaccgatt tccgtaacag cgttgacgtg atgaccacgg
      781 aaaccacctg tttaagctcc gtctggcaaa ctgatgaaga agtgcataac tggctggcgc
      841 tgcacggtcg cggccaggat tactgccagc ttaaccctca accgatggcg tactacgatg
      901 gctgcatcag cgttgattta agcgccatca aaccaatgat tgcgctgccg ttccacccga
      961 gcaacgtgta tgaaatcgac acactgaacc agaacctgac cgacattctg cgtgagattg
     1021 aaattgagtc cgaacgcgtg gcgcacggta aagccaaact ctcgctgctg gataaagtgg
     1081 aaaatggtcg cctgaaagtg cagcagggga ttatcgcggg ctgttctggc ggtaactacg
     1141 aaaacgtcat cgcggcggcg aatgcactgc gcggtcaatc ctgtggcaat gacaccttct
     1201 cgctggcggt ttacccgtca tcacagccgg tgtttatgga tctagccaaa aaaggtgtgg
     1261 tagcagattt gattggcgca ggcgcaatca tcagaaccgc attctgtggc ccatgctttg
     1321 gcgcgggcga tacgccaatc aacaacggtt tgagtattcg ccacaccacg cgtaacttcc
     1381 cgaaccgcga aggctctaag ccagccaatg ggcagatgtc agcggtggcg ctgatggacg
     1441 ctcgttctat cgctgcgact gcggcaaacg gtggctattt aacctctgcc agcgaacttg
     1501 attgctggga caacgtgccg gagtacgcct tcgatgtaac gccgtataaa aaccgtgttt
     1561 atcagggctt tgtgaaaggg gcaactcagc aaccgctgat ttacggaccg aacattaaag
     1621 actggccgga attgggtgcg ctgactgaca atatcgtcct gaaagtgtgc tcgaagatcc
     1681 tcgacgaagt caccaccacc gacgaactga ttccttccgg tgaaacctct tcttatcgtt
     1741 caaatccgat tggtctggcg gggaatgtca gcgagctgac agaggtgttt gcgcgcatta
     1801 agcagattgc tggtcaggag catattgatc cgctgcaaac tgaaattggc agcatggtct
     1861 atgcggtgaa accaggcgat ggttctgcgc gtgaacaggc ggcgagctgc cagcgtgtga
     1921 ttggcggtct ggcgaatatt gccgaggagt acgcgactaa acgctaccgt tctaacgtca
     1981 tcaactgggg gatgttaccg ctgcagatgg cggaagtgcc aacctttgaa gtgggggatt
     2041 acatttacat ccctggcatt aaagcggcgc tggataatcc gggtacgacg tttaaaggtt
     2101 atgtgatcca tgaagatgcg ccggtaacgg aaattacgct ttacatggaa agcctgacgg
     2161 ctgaagagcg cgagattatc aaggcgggta gtttgattaa cttcaataaa aaccatcaga
     2221 tgtaaaaagc gccatgtgaa tgtaggtcgc attcggcact tattgtcgga tgcgatgctg
     2281 gcgcatctta tccgacctac aaatcgcatc gaaccgtagg ccggataagg cgtttacgcc
     2341 gcatccggca aatagttaat agctcttact tcttcgcctc tgcaaccact ttgctgccca
     2401 cgccgcggtt attgtattcc cacatgcggt tgtagttagt gtcattcaga ttgcgctgta
     2461 tttcgtcgtt atcatctacg ctgccggtat tacccgcaaa cggacgatta gagatcaccg
     2521 catcggccca cggtttagcc gtgttaaaac cttcgttgat ggcgctatca cggatcacca
     2581 cctgaccgtt ggtattagca tcaacatcca gcgagcggcc cagttgcgcc acgccatcac
     2641 cggaagcatt gaaacggctg tttacggcga ggaaaccgta atagatgtta gacagcgtag
     2701 ccggtgcaaa cacatatgct tcttgctggg tacgtgagtt caccacgcgg aattcggtgt
     2761 tatcgaacac cactgcgccg cgaccagaaa cgatatccac atccccttca atatagctgt
     2821 tggtcaccag cgtacgcggc tgacggttgg tttccagacg gttctgcaca ccgctgttgg
     2881 tgacaaagaa ggtgttctga cgaccgagaa tgttaacgtt gttaatctgt acctggtcac
     2941 cgtcagtacg cagtgctacc gccggatggt tacctgcatc tacactatcg cccagcgtgt
     3001 tttcgatggt cagattttgc agttgcagtc cattgttttg tgaccagaag accgcagaac
     3061 acagaacacc gatactgtcg ctgcgtttgc tctggcagct atcgtacata taccacgctg
     3121 gtttacctgg catatatttg ccgcgcgggt tgacgtcgtg acgccagtcg gcagggctca
     3181 tgcccccatc aagggaaagc ccaatcttca catcaatcgg tttttcacct gtaccgtaca
     3241 gagtgattcc acctggagca gcagggacat acaccgttcc ctgatactca ccaggcatca
     3301 cggcaatata ctggcgcttg ttggtgcgct tgataattgc cgcatctacc gccgcctgaa
     3361 tcgtggtatg cgttacacct tgagtacccg ccgggccgac aacaaagtca ggttgcgcag
     3421 gcagggttat cggggacgga ttccacgctg cagcacctgg tgtcagggat gcaaaatagt
     3481 gttgcgcatc gaaattcagc gcttcttttg ccgacagaat cgggcgagaa gaggtaccag
     3541 gcgcagtttg atcagaagga cgttgatcgg gtggtgttga gctacaggcg gtcagcgtca
     3601 cgccaaaagc caatgccagc gccagacggg aaactgaaaa tgtgttcaca ggttgctccg
     3661 ggctatgaaa tagaaaaatg aatccgttga agcctgcttt tttatactaa gttggcatta
     3721 taaaaaagca ttgcttatca atttgttgca acgaacaggt cattatcagt caaaataaaa
     3781 tcattatttg atttcaattt tgtcccactc cctgcctctg tcatcacgat actgtgatgc
     3841 catggtgtcc gacttgtgcc cgagaagatg ttgagcaaac ttatcgctta tctgcttctc
     3901 atagagtctt gcagacaaac tgcgcaactc gtgaaaggta ggtggatccc cttcgaagga
     3961 aagacctgat gcttttcgtg cgcgcataaa ataccttgat actgtgccgg atgaaagcgg
     4021 ttcacgacga gtagatgcaa ttatggtttc tccgccaaga atctctttgc atttatcaag
     4081 tgtttccttc attgatatcc cgagagcatc aacatgcaat gttgtaggga tggcaatttt
     4141 tacgcctgtt ttgctttgct cgacataaag atatccatct acgatatcag accacttcat
     4201 ttcgcataaa tcaccaactc gttgcccggt aacaacagcc agttccattg caagtctgag
     4261 ccaacatggt gatgattctg ctgcttgata aattttcagg tattcgtcag ccgtaagtct
     4321 tgatctcctt acctctgatt ttgctgcgcg agtggcagcg accgggtttg ttgttatatg
     4381 gccttcagct attgcctctc ggaatgcatc gctcagtgtt gatctgatta acttggctga
     4441 tgccgccttg ccctcgtcta tgtatccatt gagcattgcc gcaatttctt ttgtggtgat
     4501 gtcttcaagt ggagcatcag gcagccccct ccttattgct ttaattttgc tcatgtaatt
     4561 tatgagtgtc ttctgcttga ttcctctgct ggcgaggatt ttttcgtagc gatcaagcca
     4621 tgaatgtaac gtaacagaat tatcactgtt gattctcgct gtcagaggct tgtgtttgtg
     4681 tcctgaaaat aactcaatgt tggcctgtat agcttcagtg attgcgattc gcctgtctcg
     4741 gcctaatcca aactctttac ccgtccttgg gtccctgtag cagtaatatc cattgtttct
     4801 tatataaagg ttagggggta aatcccggcg ctcatgactt cgccttcttc ccatttctga
     4861 tcctcttcaa aaggctacct gttactggtc gatttaagtc aacctttacc gctgattcgt
     4921 ggaacaaata ctctcttcca tccttaaccg gaggagggaa tatcctgcac tcgcgtaccc
     4981 atcgacgaac tgtttcaagg ctccttgggc gtcgctggcg tgcgttccac tcctgaagtg
     5041 tcaagtacat cgcaaagtct ccgcaattac tcgcaaggga gcgagtattg gtttattttc
     5101 tccaacaaaa aaggagccga agctcctttg atgattaaaa ttcgaattgt ctcgcccgaa
     5161 ggcttttcag cattggtttt gcccgttcga aaagggcgct tgcttggtca agtcgtgtcg
     5221 cctcccttag cagtacatct ctgtttttcg tcaccgtgta aaaagtttca aacgcgatgt
     5281 catacagctt gctcgtgtat gatgagttca gttccttcaa tatcgggtac agacgtttgc
     5341 agatgtcctg cgcattctcc atctgtacct gcatatagca aaggaggatg atttcttcgt
     5401 ctgtgaattg ctgcgctggt tgcatgctgc gaagtttctt ttcgcactcg atgaagtatc
     5461 ggcgtatctg gcggcctttt tcgttacgct cgaccatcgc cgtttctttg gctgtatcga
     5521 gggtgaggtg atagtctttg ctacggcgat cgcctccacg tcctttgatc cccctgtttt
     5581 ggtgaaccaa aatgtaatcg acatcaacgg caaagccgta ttctgttatg cgatttttaa
     5641 tccatgtaga gaagtcacga cgtacttcca aaaattcgtg taaatcacgg gcattaacga
     5701 gaagagttgt ttcgttggat atagtgccgt tgaatacggg gatgagttga ctggtcattt
     5761 ttatgtcctt tgcatttgtt cagatagccc tgtgttcagc agggcggtca ggtacttgaa
     5821 caccgtgcaa agtcggcccg tatccttaac ctttcggctg tttttcggta tacgcgctac
     5881 ccgaccatat ctgaaaaatg gacataaaaa atccgcatga ctgacgggtg cggtttccgc
     5941 tttgcaaggt gtgttcagca ccatgaagcg gaatatagcc ccgttaatgc ggtgttgtca
     6001 aatcatgtag gccttatctt gctgtaagcc gcgccattcg ggcttttccc cacatttggg
     6061 gaaaactatc ttgtcaaaat catgcagcct ttcgctctcc ttcgatgagt tcctctattt
     6121 tgtgcactcc agcctcgttg taccgaaatg tttcaacctg cttgtcattg tgcgccgatt
     6181 tgtcgatgaa ccacttcccg tacttttctg ttttgagcat gtaggtgttg gctatgcgtc
     6241 ctaccttgtt ggctgaaata ccgtagcgct ggccgacttc cgtagcagag tagtagtgct
     6301 ctgagatagc tggcagaggt acaacatcgc gaccaacaat cgggttgaac actccagcta
     6361 ctgctgcctg ctttgattct gaggacagat tcgggaacat attcaccagt cgatccgcca
     6421 cgttcaaagc tatttccatg gctcgcgcac ggtctaggct actttctggt tgcggtactg
     6481 gttgaggatg gttggcaacc ttttcgcgct gattgaagta gccgtcctcc agtttttcga
     6541 acacgtccca tgcctgatcc gtttcgagca tcttcgcgtg acgggcggct ccgcgctctg
     6601 tccagaggat gaggtggcgt gcatttgggg cacttttcac aaagtagttt aaagctactt
     6661 tgtgttttag cgcgcgcagc tcgtcaccaa cgaccttgta gaagtgtacc ccctcaacaa
     6721 agcgcgactt gttttcatga tggttttgcc ggatgcgcac aacatccgtt ccgtacaact
     6781 gcgccaatgt ttggtgggta gttgttttta aagtcagctg ctaacacata gcattgtcgc
     6841 tctcccattt ctggcattcg ctcactacag cttatccagg catccggagt taccggcact
     6901 ggctgggcgg tataaagcgg tgttatatct gcccgaaaat tacatgcttt atgcagccgc
     6961 acccaccgtt cgacttctgc tttgtcagaa tacataccag tgaacgtgtt atattcacgg
     7021 tcaatttgcg tgaaggttac cttccacgcc accggctctg ctgtcagcga tgccagagca
     7081 atttcataag cacggcgttc aacattgtct cgcacatcca aactgctgat acgctctttg
     7141 atttctttaa tcagttcttt gtcgttcact tatgcctccg attcccggat gtcacccaaa
     7201 tcaacgctac aatgccaacc agtgcaacga gcgcaaaact atcaagcatc tccaacctct
     7261 ttaacggtaa ctgtctgtgc ctccagttct gctatgcgct tttctgaggc ttcaagtaac
     7321 gcctgcttat cgcgtagcgc ttcttccagt tcagcaacat
//
LOCUS       DAJTNM010000161         7325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTNM010000161 DAJTNM010000000
VERSION     DAJTNM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.161"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            19..1077
                     /locus_tag="OO172_003712"
     CDS             19..1077
                     /locus_tag="OO172_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5513722.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1120..1623
                     /gene="yeaK"
                     /locus_tag="OO172_003713"
     CDS             1120..1623
                     /gene="yeaK"
                     /locus_tag="OO172_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5513723.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVIHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1624..1728)
                     /locus_tag="OO172_003714"
     CDS             complement(1624..1728)
                     /locus_tag="OO172_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513724.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1898..2344
                     /locus_tag="OO172_003715"
     CDS             1898..2344
                     /locus_tag="OO172_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5513725.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2301..3122)
                     /gene="nimR"
                     /locus_tag="OO172_003716"
     CDS             complement(2301..3122)
                     /gene="nimR"
                     /locus_tag="OO172_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR5513726.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3219..4400
                     /gene="nimT"
                     /locus_tag="OO172_003717"
                     /pseudo
     CDS             3219..4400
                     /gene="nimT"
                     /locus_tag="OO172_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4455..4802
                     /locus_tag="OO172_003718"
     CDS             4455..4802
                     /locus_tag="OO172_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5513727.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4824..5078)
                     /gene="yoaF"
                     /locus_tag="OO172_003719"
     CDS             complement(4824..5078)
                     /gene="yoaF"
                     /locus_tag="OO172_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5513728.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5261..6286
                     /gene="dgcP"
                     /locus_tag="OO172_003720"
     CDS             5261..6286
                     /gene="dgcP"
                     /locus_tag="OO172_003720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR5513729.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6319..6417
                     /locus_tag="OO172_003721"
     CDS             6319..6417
                     /locus_tag="OO172_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5513730.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6420..6494
                     /gene="yoaJ"
                     /locus_tag="OO172_003722"
     CDS             6420..6494
                     /gene="yoaJ"
                     /locus_tag="OO172_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5513731.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6546..6977)
                     /locus_tag="OO172_003723"
     CDS             complement(6546..6977)
                     /locus_tag="OO172_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513732.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKALEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(6971..7198)
                     /locus_tag="OO172_003724"
     CDS             complement(6971..7198)
                     /locus_tag="OO172_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5513733.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         1775 a         1773 c         1770 g         2007 t
ORIGIN      
        1 gctgctgagc agtcggaagt gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag
       61 tctgccaata atacccatgg cttaaacctg actgcccata aatacccggg cttacgcggc
      121 acactacaaa ccgcatccac tgactgcgac acaattgtgg aagctgcagc actattaccc
      181 gcttttgatc aggcagtgga aggcaaccgc caccaggatg attacggttc aggtcttggg
      241 atagccgaag agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat
      301 gagccggtta atgttgaata ctttgcgatg aataactggt ccttcctgca gtcaggaagt
      361 attggcatcg atcgcaaaga tattgaaaag gtatttaccg ggcgtaccgt attgtcgagc
      421 atttaccagg atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtatatgtc
      481 gcagggcagc taaaagggat tgtgctgctg gatattaaca aaaacaattt gcggaatatc
      541 ttttacactc atgaccgccc tctcctctgg cgttttctca atgtcacgct aaccgatacc
      601 gattcggggc gcgacattat catcaaccag agcgaagata atctgttcca gtatgtcagt
      661 tacgtccatg acttaccggg cggcattcgt gtctcgttat ccattgatat tctttacttt
      721 atcacgtctt cgtggaaaag cgttctgttc tggattttga cggcgttaat tttgctgaat
      781 atggtccgga tgcacttccg tttataccaa aatgtgtcgc gagaaaatat tagtgatgcg
      841 atgactggac tgtataatcg caaaatttta acccctgaac tggagcagcg gttgcagaaa
      901 ctggtgcaat ccggttcttc ggtgatgttt attgcaattg acatggacaa gttaaagcaa
      961 ataaatgaca acctcggtca tcaggagggg gatttagcga ttacgttaca tgatgcgtat
     1021 aaagcttccg atgagcgttt atatgtcaat aagcagaaca aaaacagccg ttcatgataa
     1081 ccttctgtgg ttgtttgctt gtaatctcag gagcgtgaaa tgactgaaat ggctaaagga
     1141 agcgtgacgc atcagcagtt aatcgcttta ttatcacaag aaggtgctaa cttccgcgtc
     1201 gttatccatg aagcggtagg taaatgtgaa gcggtgtcag aaattcgtgg caccgctcta
     1261 ggccagggtg ctaaggcact ggtatgtaaa gtcaaaggca atggcgtaaa tcaacatgtt
     1321 ctggcgatcc tcgcagccga tcaacaggcc gatctgagcc aactcgccag ccatataggc
     1381 ggattacgcg cttcgttggc cagcccggcg gaagttgatg aactcacagg ctgtgtcttc
     1441 ggcgcgatcc cccctttcag cttccatcca aaactcaaac tggttgccga ccctctactc
     1501 ttcgaacgat ttgatgaaat cgccttcaat gcagggatgc tggataaatc cgttattttg
     1561 aaaaccgcgg attatttgcg cattgcgcaa ccagaactgg tcaatttccg ccgcactgcg
     1621 tagctagcct atccgttcaa taagcagaac ggacaaaacc agcacaaccg caatagcaaa
     1681 aaacgatgat gtgataatca gtgtctcgac gaacatttga tcgttcatag catgcccccg
     1741 ggagatagct gttttcacgt tattgttagc gtgcacaaat ggcagtttga tgacagttcg
     1801 cctgattttt tatgcaaaaa acgtaaagat tttctacttc cttcctgcag caagcgtaaa
     1861 gtaagcaggc ttattatttt ttggcaagga aaccacgatg tttgatgtca ctttgctgat
     1921 cctgctcggg ttagctgcgc tgggctttat cagtcataac accactgtcg ccgtttcaat
     1981 tctggtgttg atcattgtcc gcgtcacacc gttaagcacc tttttcccgt ggattgaaaa
     2041 acaggggcta agtatcggga taatcattct gacgattggc gttatggccc ccatcgccag
     2101 cgggacgcta ccgccctcaa ccttgattca ctcgtttctg aactggaaat cgctggtggc
     2161 aattgcagta ggcgttattg tctcctggct gggtgggcgc ggcgtgacgt taatgggcag
     2221 ccagccgcaa ctggttgctg ggctgctggt gggcactgtt ttaggcgtag cgctgtttcg
     2281 cggcgtaccg gttggcccgc ttattgccgc gggtctggtt tcgctgattg tggggaaaca
     2341 gtagttaatc ccgcaatata tcggcctggc gtttgcccta ggcctttctt aaacatagta
     2401 ataaaggccg ttgttgaatc ataccccagc gtatgagcaa ctttctgtac agtatcgcct
     2461 ttcactaatc cctgaagcgc cataatcagc tgtaactgct gacgccactg gcgaaaactc
     2521 aaccccgtct ctttcactat cagtcgggca agattgcgtt cactcatcgc aaaaaagcca
     2581 gcccattgcc ccaatgaccc ccactcgacg ggccctttcg ccatcatctc caccatcgtg
     2641 cggattttag gatgagaaga aacgggcagg tgcagctgtt gttgtggctg ctgagggagt
     2701 tcatcaaaca ggacctgaat taatctgcgg gtcatcggct cagcacgctg agtcgtcgtc
     2761 ctgtttgcca gggttaaaat taattcccgg cataagggcg atattttcaa tgtgcaacat
     2821 gttgtcggca ttgtcactgc gctgggttcg ataaacagaa aacagagttc agcgttagct
     2881 gtcacttgat tgctatgctt caccccgccc ggtatccaaa ccgcatattg tggcggcacc
     2941 atccacaaag cattttccac cgtacaggta attgcaccat gtaatgctag tatcagctgt
     3001 cctttgcggt gctgatgtac aggactggtt aactcatccg ttccggcgtg aatacaaaat
     3061 gcgaccgcgg cttcatggtg tcggtcgggt tcatagccat tgagattcaa cctgtgcatc
     3121 attttgtccg aacttagcga taatttgtca ttttagcttg attcaacata acaataaaaa
     3181 cggtaaggta cagcctcgtt tgtaacaatg agaagcatat gacctgttca acttcattaa
     3241 gcggcaaaaa caggattgtc cttatcgctg gcattctgat gattgccaca acattacgcg
     3301 tcacctttac cggcgcagcg ccgttactgg atacgattcg ttccgcttac tcgctgacga
     3361 cagcgcaaac cggcttatta accaccctgc cattattggc ctttgcgcta atctcacctc
     3421 tggctgcccc ggtggcgcga cgttttggta tggaacgtag cctgtttgcc gcgttacttt
     3481 tgatctgtgc aggtatcgca attcgttctc tcccttcgcc ttacttatta tttggcggta
     3541 cagcggtcat tggcggtggg attgcattag gcaatgtctt actgccagga ttaattaaac
     3601 gcgatttccc tcattccgtc gccagactta ccggcgcata ttccctgaca atgggagctg
     3661 cagcggcact gggatcggct atggtcgtgc cgctggcttt gaacggtttt ggctggcaag
     3721 gcgcgttgtt catgctgatg tgttttcctc tgctggctct ttttttatgg ctgccacagt
     3781 ggcgaagtca gcaacatgca aatttgagta cctcacgcgc cttacatact cggggtatct
     3841 ggcgttcgcc acttgcctag caggtaacat tgtttcttgg gatcaactca ctggtctatt
     3901 acgtgattat tggctggctt ccggcgatcc tcatcagtca cggctatagc gaagcacagg
     3961 cgggttcact gcatggtttg ctgcaactag ccacagcagc tcccggtttg ctgatcccac
     4021 ttttcttaca tcatgtgaaa gaccagcgtg gtattgcagc gttcgttgct ttgatgtgcg
     4081 cagtgggcgc ggttgggctc tgctttatgc cagcgcacgc gatcacctgg actctgcttt
     4141 tcggttttgg ttccggcgca acaatgatac tggggttgac gttcattggt cttcgggcaa
     4201 gttctgcgca tcaggcggcg gcactctcgg ggatggcaca atccgtcggg tatttgctgg
     4261 cagcctgtgg gccgccgcta atgggtaaaa tacacgatgc taacggtaac tggtccgtac
     4321 cactgatggg tgttgcaata ctttcactac tgatggcgat tttcggactt tgcgccggga
     4381 gagacaaaga aattcgctaa tatccggtgc tatagtgacg tgacaaatct tgcgtgaaag
     4441 ggagaacaaa cacgatgaat attcagtgca aacgcgttta tgatccggct gaacagagcg
     4501 atggttatcg cgtactggtc gaccgcctct ggccgcgcgg tatcaaaaaa accgatttag
     4561 cccttgatga gtgggataaa gaaatcacgc cgtcaacgga actgcgcaaa gcctttcacg
     4621 gcgaagtcgt cgattttgca acctttcgcg agcaatatcg cgcagaactg gcgcaacacg
     4681 agcaagaagg aaagcggctg gcggacatcg ccaaaaaaca gccgctgacc ctgctctacg
     4741 cagcaaaaaa caccacgcag aaccatgcgc tggtgctggc cgactggcta cgtagcttgt
     4801 gattttagta cggcatccgg cggttatttt tcaccagccg gatggtcccg tcgccacaat
     4861 gcccattcat caatcgtttc accgcccggt aatttgcaat tgttgctgac cccttgcgct
     4921 gtctgcactg gaatgagcgt cccgcccttc tgctggcaat agaccgacgc cggatttgcc
     4981 ataccaatct gcggcggttt aggtgcttct ggctgagaag gggttgaaca accagccagg
     5041 accagcaagc aaggcagaac aaaactgata attttcattt attgatctca catatttatc
     5101 caagcttaga gtatcgcggt atcgttttgt tttgcagcac tatttttatt acattcactc
     5161 aaaacatatt acgtcttgtt tcatctttgt tgatgatgtt ttatcatgcc tgcaaagatt
     5221 aaataatcag cacttacccg ccgtatcctg gagttgttcc gtgtcagatc agattatcgc
     5281 ccgcgtctcg caatcccttg ccaaagaaca gtcactggaa agtctggtcc gacagcttct
     5341 ggagatgctg gaaatggtca ctgatatgga atcaacctac ctgaccaaag tggatgtcga
     5401 agcgcgcctg caacatatta tgtttgcccg taacagccag aaaatgcaca tcccggagaa
     5461 ttttaccgtc tcgtgggatt actcgttatg caaacgcgcc attgatgaaa actgcttttt
     5521 cagcgatgaa gtccctgacc gttggggcga ctgtattgcg gcacgcaatc ttggcatcac
     5581 cacatttctg agcacgccaa ttcacttacc ggatggatca ttctatggca cgctttgcgc
     5641 cgccagcagt gagaagcgcc agtggagtga acgcgcggaa caggttttac agttattcgc
     5701 cggactgatt gcacaatata ttcaaaaaga ggcactggtt gaacagctgc acgaagccaa
     5761 tgctgcgctg attgcgcaat cgtataccga ctcgttaacc gggctaccga atcggcgggc
     5821 gatttttgaa aatctgacga cgctgttttc cctcgcccgg catcttaacc ataagataat
     5881 gatcgcgttt atcgatctgg ataacttcaa attaatcaat gatcgttttg gtcataatag
     5941 tggcgatctg tttctcattc aggttggcga gcgccttaat acgctccagc aaaatggcga
     6001 agttattggt cgtctcggtg gtgatgagtt tttggttgtt tcactaaaca acgagaatgc
     6061 ggatatttcg tcgctgcgag aacgtattca acagcaaata cgtggagaat atcacttagg
     6121 tgatgttgat ttgtattatc ccggtgccag tcttggcata gtagaagtcg atcctgaaac
     6181 aaccgatgca gacagtgccc tgcatgctgc cgatattgcg atgtatcagg agaaaaaaca
     6241 caaacagaaa acaccttttg tcgcgcatcc agcgctacat tcctgaggcg tattcacatc
     6301 cttttgattg gtgataacat gcgaatcggt attatttttc cggttgtaat cttcattaca
     6361 gcggtcgtat ttttagcatg gttttttatt ggcggctatg ctgccccggg agcataaaga
     6421 tgaaaaaaac aacgattatt atgatgggtg tggcgattat tgtcgtactc ggcactgagc
     6481 tgggatggtg gtaacgtcac ctctaaaaaa tagcaaaggc tgcctgtgtg cagcctttgt
     6541 gcaatttaag cgttaacttt taatcttcct gtagataaat agcacgacaa tcgcaccaat
     6601 aacggctgaa gcatttcgtc tgcagtctga accgcaggca accatttttc agcaagtctg
     6661 cgctgggaat cgctggcttt atcccatttg ctccacagtg cctcacgatc gtaatcgtgc
     6721 gcttccagcg tggggcgggc gatttcaaaa tacccctggc ttgtttctat ttgctcagat
     6781 tttaataaca actcaacacg tgctgccgct ggtagaactg atatatgaaa ttccaaagcc
     6841 tttgaacccg tacgcttgcg acgtagttct ggatgattgg ctgcaagctt ttcaagattg
     6901 cggcgaatac tcacctcatg tttaggaaag catgacattc ctacgcattc ttgcgctgtt
     6961 acccaatggg ttaacatgat gttttctcca tataccgaga aggccaaatc tgggctggtt
     7021 ccatatccaa atatctagca atgattgctt cgtatcgtgg tatatggcag cgaaatacgt
     7081 tacgcatagt gtcaggcttt aagcctgcct ctctggataa ttgagatagt gtcttcccct
     7141 tcatacgaac agcagccttt atctgctctg cgtgccagtc ttggtttttt gctgacattc
     7201 tgcgatcctt actctcttaa cgttttcggt aactgatacc ggctaccgct atcgggaaaa
     7261 acgatatcgc agaaaaatgc gcatttcaag agctatgcac atttttattt taaccaatgc
     7321 aaatc
//
LOCUS       DAJTNM010000162         7293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTNM010000162 DAJTNM010000000
VERSION     DAJTNM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.162"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            94..1205
                     /gene="yneK"
                     /locus_tag="OO172_003725"
                     /pseudo
     CDS             94..1205
                     /gene="yneK"
                     /locus_tag="OO172_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1355..2545
                     /gene="ydeA"
                     /locus_tag="OO172_003726"
     CDS             1355..2545
                     /gene="ydeA"
                     /locus_tag="OO172_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5513734.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2570..3235)
                     /gene="marC"
                     /locus_tag="OO172_003727"
     CDS             complement(2570..3235)
                     /gene="marC"
                     /locus_tag="OO172_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5513735.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3447..3881
                     /gene="marR"
                     /locus_tag="OO172_003728"
     CDS             3447..3881
                     /gene="marR"
                     /locus_tag="OO172_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5513736.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3901..4306
                     /gene="marA"
                     /locus_tag="OO172_003729"
                     /pseudo
     CDS             3901..4306
                     /gene="marA"
                     /locus_tag="OO172_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
     gene            4338..4556
                     /gene="marB"
                     /locus_tag="OO172_003730"
     CDS             4338..4556
                     /gene="marB"
                     /locus_tag="OO172_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5513737.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4587..5486)
                     /gene="eamA"
                     /locus_tag="OO172_003731"
     CDS             complement(4587..5486)
                     /gene="eamA"
                     /locus_tag="OO172_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5513738.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5750..6868
                     /gene="ydeE"
                     /locus_tag="OO172_003732"
     CDS             5750..6868
                     /gene="ydeE"
                     /locus_tag="OO172_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5513739.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            6995..7090
                     /gene="mgtS"
                     /locus_tag="OO172_003733"
     CDS             6995..7090
                     /gene="mgtS"
                     /locus_tag="OO172_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5513740.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1904 a         1775 c         1659 g         1955 t
ORIGIN      
        1 acagcgccac gcatcacctg cctttgatag ttcttgcagc tcaaatgccc tatatagtga
       61 tgttccgatg acttatgact atatggggca aatatggtta cgccagtaag catcagcaat
      121 tacatatctc ttcctgatga ttttcccgct cgtaatattg cacctcaagt aaaagaagtt
      181 ttaaaggatt ttattgatgc actcagtacc ataatatgtg atgaagagtg gcgcacgtct
      241 ttaaacacca actcagccac aaaaaagata tttaacaacc ttgataactt atcatatatt
      301 cagaggactt cttttcgggg taacgacaca ctatacaatg aaaaggttca gtttaaactc
      361 acttatcccg tcaagaatgg aagacacaaa gaaaatattg aatttcaggt agtaataaat
      421 ttaagcccta tttagataat tttcgccatg atggagaaat taatattttt tgcgctccca
      481 acccaaagcc tgtcactatg gggcgcgtat ttcagaccgg cgtcgagcgt gtgctctttc
      541 tgtttatgaa tgattttatt gaacaatttc caatgatcaa ccttggcgcc cccatcaaaa
      601 gagcgcatac accacatatt gaacccctgc ctccggatca ccataccgct gcagattact
      661 tacgccagtt tgatttgctt gtcctgaatt ttatctctcg cggtaatttt gtcatactcc
      721 cccgattatg gaataactct gaggttcaca gatggttcgt caataaagat cctaacttga
      781 tcaccgccat tctcgacata acggacagcg aattaaaaga ggatttattg caaagcctga
      841 tggattcatt aggttctaac aaacatgtac aacccgaagt ctgcatctgc ttcttatccc
      901 ttttagcaga acaagaatct cctcattttc aggacttgtt tttatttttc gccaatatgt
      961 tattgcacta tcaccaattt atgaatccca atgaaagtga tctgaatgac gtgttgatgc
     1021 cagcatcatt aagtgatgat aaaattatca aacatatggc gcgcaggacc ctcaaactat
     1081 ttgtaaaaaa tgaaacgccg ccaaaagtta ctcacgaaga cctggtgaaa aacaggcctc
     1141 gctcccctgt cagaccacct atacccgcaa ccgccaaaac gccagacctc cctgaacgtc
     1201 attaaaccgt gatgttaccg actctctgac gcggaacaga atcagcgtca gagaaacgga
     1261 aaacgcgatc cagatcacaa atgcattgta ttcacatcat taaccgtttt aagatcattt
     1321 catcactttt tcgcaactca cccgataatc tgttatgaca acaaacactg tttcccgcaa
     1381 agtggcgtgg ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca acaccaccga
     1441 atttgtccct gttggcctgc tctctgacat tgcgcaaagt tttcacatgc aaaccgctca
     1501 ggtcggcatc atgttgacca tttacgcatg ggtagtagcg ctaatgtcat tgccttttat
     1561 gttaatgacc agtcaggttg aacggcgcaa attactgatc tgcctgtttg tggtgtttat
     1621 tgccagccac gtactgtcgt ttttatcgtg gagctttacc gttctggtga tcagtcgcat
     1681 tggtgtggct tttgcacatg cgattttctg gtcgattacg gcgtctctgg cgatccgtat
     1741 ggctccggcc gggaagcggg cacaggcctt gagtttaatt gccaccggta cagcactggc
     1801 gatggtctta ggtttaccgc tcgggcgcat tgtggggcag tatttcggct ggcgaatgac
     1861 cttcttcgcg attggtattg gggcgcttgt tacccttttg tgcctgatta agttacttcc
     1921 cttactgcct agtgagcatt ccggttcatt gaaaagcctc ccgctattgt tccgccgccc
     1981 ggcattgatg agcatttatt tgttaactgt ggtggttgtc accgcccatt acacggcata
     2041 cagctatatc gagccttttg tgcaaaacat tgcgggattc agcgccaact ttgccacggc
     2101 attactgtta ttactcggtg gtgcgggcat tattggcagc gtgattttcg gtaaactggg
     2161 taatcaatat gcgtctgcgt tggtaagtac ggcgattgcg ctgttgctgg tgtgtctggc
     2221 actgctgcta cctgcggcga acagtgaaat acacctcggg gtgctgagta ttttctgggg
     2281 gatcgcgatg atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc tggcaccaga
     2341 tgctaccgac gtcgcgatgg cgctattctc cggcatattt aatattggaa tcggggcggg
     2401 tgcgttggta ggtaatcagg tgagtttgca ctggtcaatg tcgatgattg gttatgtggg
     2461 cgcggtgcct gcttttgccg cgttaatatg gtcaatcatt atatttcgcc gctggccagt
     2521 gacactcgaa gaacagacgc aatagttgaa aggcccattc gggccttttt taatggtacg
     2581 ttttaatgat ttccaggatg ccgttaataa taaactgcac acccatacat accagcagga
     2641 accccatcag gcgggagatc gcttcaatgc cacccttgcc caccagccgc ataattgcgc
     2701 cggagctgcg taggcttccc cacaaaataa ccgccaccag gaaaaagatc agcggcggcg
     2761 caaccatcag tacccaatca gcgaaggttg aactctgacg cactgtggac gccgagctaa
     2821 taatcatcgc tatggttccc ggaccggcgg tacttggcat tgccagtggt acaaaggcaa
     2881 tattggcact gggttcatct tccagctctt ccgacttgct tttcgcctcc ggtgaatcaa
     2941 tcgctttctg ttgcggaaaa agcatccgaa aaccgataaa cgcgacgatt aagccgcctg
     3001 caattcgcag accgggaatc gaaatgccaa atgtatccat caccagttgc ccagcgtaat
     3061 acgccaccat catgatggca aatacgtaca ccgaggccat caacgactga cgattacgtt
     3121 cggcactgtt catgttgcct gccaggccaa gaaatagcgc aacagttgtt aatgggttag
     3181 ctaacggcag caacaccacc agccccaggc caattgcttt aaacaaatct aacattggtg
     3241 gttgttatcc tgtgtatctg ggttatcagc gaaaagtata aggggtaaac aaggataaag
     3301 tgtcactctt tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat ttagcaaaac
     3361 gtggcatcgg tcaattcatt catttgactt atacttgcct aggcaatatt atcccctgca
     3421 actaattact tgccagggca actaatgtga aaagtaccag cgatctgttc aatgaaatta
     3481 ttccattggg tcgcttaatc catatggtta atcagaagaa agatcgtctg cttaacgagt
     3541 atctgtctcc gctggatatt accgcggcac agtttaaggt gctctgctct atccgctgcg
     3601 cggcgtgtat tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg ggagcactga
     3661 cccgtatgct ggatcgcctg gtctgtaaag gctgggttga aaggttgccg aacccgaatg
     3721 ataagcgcgg cgtactggta aaacttacca ccagcggcgc ggcaatatgc gaacaatgcc
     3781 atcaattagt tggccaggac ctgcatcaag aattaacaaa aaacctgacg gcggacgaag
     3841 ttgcaacact tgagcatttg cttaagaaag tcctgccgta aacaaaaaag aggtatgacg
     3901 atgtccagac gcaatactga cgctattacc attcatagca ttttggactg gatcgaggac
     3961 aacctggaat cgccactgtc actggagaaa gtgtcagagc gttcgggtta ctccaaatga
     4021 gcgttcgggt tactccaaat ggcacctgca acggatgttt aaaaaagaaa ccggtcattc
     4081 attaggccaa tacatccgca gccgtaagat gacggaaatc gcgcaaaagc tgaaggaaag
     4141 taacgagccg atactctatc tggcagaacg atatggcttc gagtcgcaac aaactctgac
     4201 ccgaaccttc aaaaattact ttgatgttcc gccgcataaa taccggatga ccaatatgca
     4261 gggcgaatcg cgctttttac atccattaaa tcattacaac agctagttga taacgtgaca
     4321 acgtcactga ggcaatcatg aaaccacttt catccgcaat agcagctgcg cttattctct
     4381 tttccgcgca gggcgttgcg gaacaaacca cgcagccagt tgttacttct tgtgccaatg
     4441 tcgtggttgt tcccccatcg caggaacaac caccgtttga tttaaatcac atgggtactg
     4501 gcagtgataa gtcggatgcg ctcggcgtgc cctattataa tcaacacgct atgtagtttg
     4561 ttctggcccc gacatctcgg ggcttattaa cttcccacct ttaccgcttt acgccaccgc
     4621 aagccaaata cattgatata cagcccggtc ataatgagca ccgcacctaa aaattgcaga
     4681 cccgttaagc gttcatccaa caatagtgcc gcacttgcca gtccgactac gggcaccagt
     4741 aacgataacg gtgcaacccg ccaggtttca tagcgtccca gtaacgtccc ccagatccca
     4801 taaccaacaa ttgtcgccac aaacgccaga tacatcagcg acaagatggt ggtcatatca
     4861 atagtaacca gactgtgaat catggatgcg gaaccatcga gaatcagcga ggcaacaaag
     4921 aagggaatga ttgggattaa agcgctccag attaccagcg acatcaccgc cggacgcgtt
     4981 gagtgcgaca tgatcttttt attgaagatg ttgccacacg cccaactaaa tgctgcagcc
     5041 agggtcaaca taaagccgag catcgccaca tgctgaccgt tcagactatc ttcgattaac
     5101 accagtacgc caaaaatcgc taaggcgatc cccgccaatt gtttgccatg cagtcgctcc
     5161 ccgaaagtaa acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa
     5221 gccagtccag caggcatacc aaagttaatg gcgcagaaca gaaaggcaaa ctgcgcaaaa
     5281 ctgatggtta atccataccc caagagcaaa ttcagtggta ctttcggtcg tgcaacaaaa
     5341 aagatggccg gaaaagcgac cagcataaag cgcaaaccgg ccagcatcag cggtggcatg
     5401 ttatgaagcc ccactttgat gaccacaaaa tttagccccc atacgaccac taccagtagc
     5461 gccaacaccc catcttttcg cgacattcta ccgcctctga atttcatctt ttgtaagcaa
     5521 tcaacttagc tgaatttact tttctttaac agttgattcg ttagtcgccg gttacgacgg
     5581 cattaatgcg caaataagtc gctatacttc ggatttttgc catgcgtccg atggttaata
     5641 acaacgacga ggcaagaagg gcgctggtag agcgtcgtag ggataagttc atttgtctgt
     5701 ccggcagtgc gtttcgttat gttttatttt agagatgtaa agaaatagca tggctacgct
     5761 gccatttatg accatttact tgagtcgcca gtacagcctg agtgtcgatc taatcggtta
     5821 tgcgatgaca attgcgctca ctattggcgt cgtttttagc ctcggttttg gtatcctggc
     5881 ggataagttc gacaagaaac gctatatgtt actggcaatt accgccttcg ccagcggttt
     5941 tattgccatt cctttagtga ataacgtgac gctggttgtg ctcttttttg ccctcattaa
     6001 ctgcgcctat tctgtttttg ctaccgtgct gaaagcctgg tttgccgaca atctttcgtc
     6061 caccagcaaa acgaaaatct tctcaatcaa ctacaccatg ctaaacattg gctggaccat
     6121 cggtccgccg ctcggcacgc tgttggtgat gcagagcatc aatctgccct tttggctggc
     6181 agctatctgt tccgcgtttc ccatgctttt cattcaaata tgggtaaagc gcagcgagaa
     6241 aatcatcgcc acggaaacag gcagtgtctg gtcgccgaaa gttttattac aagataaagc
     6301 actgttgtgg tttacctgct ctggttttct ggcttctttt gtaagcggcg catttgcttc
     6361 atgcatttca caatatgtga tggtgattgc tgatggggat tttgccgaaa aggtggtcgc
     6421 ggttgttctt ccggtgaatg ctgccatggt ggttacgttg caatattccg tgggccgccg
     6481 acttaaccca gctaacatcc gcgcgctgat gacagcaggc accctctgtt tcgtcatcgg
     6541 actggtcggt tttatttttt ccggcaacaa cctgctattg tggggtatgt cagctgcggt
     6601 atttactgtc ggtgaaatca tttatgcgcc gggcgagtat atgttgattg accatattgc
     6661 gccgccagga atgaaagcca gctatttttc cgcccagtct ttaggctggc ttggtgccgc
     6721 gattaaccca ttagtgagtg gcgtagtgct aaccagcctg ccgccttcct cgctgtttgt
     6781 catcttagcg ttggtgatca ttgctgcgtg ggtgctgatg ttaaaaggga ttcgtgcaag
     6841 accgtggggg cagcccgcgc tttgttgatt taagtcgaac acaataaaaa tttaattcag
     6901 ccttcgttta ggttatctct gctaatatct ttctcattga gatgaaaatt aaggtaagcg
     6961 aggaaacaca ccacaccata aacggaggca aataatgctg ggtaatatga atgtttttat
     7021 ggccgtactg ggaataattt tattttctgg ttttctggcc gcgtatttca gccacaaatg
     7081 ggatgactaa tgaacggaga taatccctca cctaaccggc cccttgttac agttgtgtac
     7141 aaggggcctg atttttatga cggcgaaaaa aaaccgccag taaaccggcg gtgaatgctt
     7201 gcatggatag atttgtgttt tgcttttacg ctaacaggca ttttcctgca ctgataacgg
     7261 atcgttgaca cagtagcatc agttttctca atg
//
LOCUS       DAJTNM010000163         6917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTNM010000163 DAJTNM010000000
VERSION     DAJTNM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.163"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..607
                     /gene="hchA"
                     /locus_tag="OO172_003734"
     CDS             <1..607
                     /gene="hchA"
                     /locus_tag="OO172_003734"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5513741.1"
                     /translation="YHLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSL
                     FRNPKKLADVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISL
                     CHGPAAFLALRHSDNPLNGYSICAFPDAADKQTPDIGYMPGHLTWYFGEELKKMGMNI
                     INDDITGRVHKDRKRLTGDSPFAANALGKLAAQEMLAAYAS"
     gene            complement(715..2073)
                     /gene="hprS"
                     /locus_tag="OO172_003735"
     CDS             complement(715..2073)
                     /gene="hprS"
                     /locus_tag="OO172_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5513742.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2073..2744)
                     /gene="hprR"
                     /locus_tag="OO172_003736"
     CDS             complement(2073..2744)
                     /gene="hprR"
                     /locus_tag="OO172_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5513743.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2877..3290
                     /gene="hiuH"
                     /locus_tag="OO172_003737"
     CDS             2877..3290
                     /gene="hiuH"
                     /locus_tag="OO172_003737"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5513744.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3399..4403
                     /gene="msrP"
                     /locus_tag="OO172_003738"
                     /pseudo
     CDS             3399..4403
                     /gene="msrP"
                     /locus_tag="OO172_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4404..5038
                     /gene="msrQ"
                     /locus_tag="OO172_003739"
                     /pseudo
     CDS             4404..5038
                     /gene="msrQ"
                     /locus_tag="OO172_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5296..5946
                     /gene="zinT"
                     /locus_tag="OO172_003740"
     CDS             5296..5946
                     /gene="zinT"
                     /locus_tag="OO172_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5513745.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMISIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1951 a         1563 c         1451 g         1952 t
ORIGIN      
        1 gtatcatctc catgctgcag gtttcgaatt cgaagtggcg accatttccg gtctgatgac
       61 caagtttgaa tactgggcta tgccgcacaa agatgaaaaa gtaatgccat tctttgaaca
      121 gcataaatcg ttattccgta atccgaagaa actcgcggat gttgtcgcca gcctcaacgc
      181 tgatagcgaa tatgcagcaa tctttgttcc tggtgggcat ggcgcactta ttggtttacc
      241 agaaagccag gacgtggctg ccgctttaca gtgggcaatc aaaaatgacc gttttgttat
      301 ctccctttgc cacggtccgg cggcttttct ggcacttcgc cacagcgata acccactgaa
      361 tggttattct atttgcgcat tcccagacgc cgcagacaaa caaacgccag atattggcta
      421 tatgccgggt catctcacct ggtacttcgg cgaagagttg aagaaaatgg gcatgaacat
      481 cattaatgac gacatcaccg ggcgagtaca taaggaccgt aaacgtctca ccggcgacag
      541 tccttttgca gcgaatgcgt tgggtaaact ggcggcgcag gaaatgctgg cagcttacgc
      601 gagttaatcc ctgttacatt gtgatgtaag cgcataatca atcgcagcca cgttaatact
      661 cacgacgtgg ctgcgttttt tcacggttaa cttatggcgt actgaagccc gatgttaatt
      721 tctttgcggt aacgtaattc ggaacacgtt atgcttactg agatagtgat acgtagcact
      781 tcctccatgt aattcggcaa tcgctttgac taaagaaagg ccaagtccct gacctacgga
      841 atgacgcgaa ttatctcccc gccaaaatct acggaagagt ttttcaggct cattaatttt
      901 cgttccagga ctggcgatat caatattaag agagccgttg gcatcaagaa aactggttat
      961 atgaatacgc gatttttctg gtgaatatcg aatggcgtta acaataagat tcgataacat
     1021 tcgttgcagt aaaattttat ccgcaaagat ttgttgattg cacttgacct taaagcaaat
     1081 ctctttctcg tctgaaagag attcaagata gtccaaaaga ttttcaactt ccttattgag
     1141 agaaagcgaa tccagtttca ccaaaacatt gtttttgtct gccctggcaa gaaacagtat
     1201 attctctgtt aaccgcgaaa tattttccag ctcttcaatg tttccggcaa ttgttttttg
     1261 atattcagcg atacttctga tttgactgag cgtaacctga ttctgaccca gtaatgcatt
     1321 aattggcgtt ctaagttcat gagcgagatc gtcagcaaat tgactaaggc gctcaaaatc
     1381 tttgactaaa gcatgatgca ttttattcaa cgcctgccca agaggtttta gttctctcgg
     1441 taatgcgtta acctcaaccg gcactcggct atcgttataa ttcagcgctt ccgttacgcc
     1501 actcaacttt ttgatctctc gcaaccccgt cctgattaac aacggactta atactgagca
     1561 cagtataatg gcgacaatgc aaattataat gctattaatt ttatactgtt caagcatatt
     1621 atgtctggct gaagccaatt tagccacagt aaccgttaat gatggcgaaa cttcatcaat
     1681 gttaattcgt aaagcatcta tctctgtatc attaataatg cttctgtaaa tgcctgctgc
     1741 gctgattgtc tcactagcag gtatgttatt taacatgtca tcactgacat ttgtccggtt
     1801 aacaatttta ttgatgccat cactatgaat gatcaaaata tcctgactaa catccatcat
     1861 ccggttaaag tacacaggta acgtatctgg atttacccca tcaattaaca actgcttgat
     1921 ctgcgctgtc cggttaatga gtgttgtatc atcgcgccat ttcaactcac ttgccaggcc
     1981 attatagaga gtccatacaa ttccagcgcc agcaacagac tgcagaaata taaaaagcaa
     2041 ggttaaacgg acggttatag ataatctttt cattattttt ttaccgctac gaatgaatag
     2101 cccatccccc ggattgtggc gattagcttt tcaggaaaag gatcatcaac ttttgcgcgg
     2161 agcctgcgaa tggcgacatc caccgtattg gtatcactat caaagttgat tccccaaatt
     2221 tcactcgcaa taaccgttct gggtataatc tcgccagctc tggaggccag tagccaaagt
     2281 aactgaaact ccttgcgcgt cagtgtaata ctgatattgt ccctgctgac actttgactg
     2341 acagagtcca tttttaagcc actgatttct aatgttgaat tcagagcgtg atgttgcctt
     2401 aattgtgccc gaacccttgc taacaactca gaaaatgaaa aaggttttac cagataatca
     2461 tttgccccac tgtccaaccc tctgactctg tcatcgacag aatcccttgc agtaaggcaa
     2521 ataacagggg tttgctttgc cgttcttaac gtttgtaaga tctgccagcc atccataccc
     2581 ggaagcataa tatccagaat gatcaatgca taatcatcct tcagcgcaag ataaagccca
     2641 tctctgccat cagaaacggc atcaatgaca taacccgctt cggaaagccc ctgcgttacc
     2701 cattcctggg tcctttgatt atcttcaata agtagaatct tcatgaaaat aatatgccat
     2761 aaatcatatg ttatagaaac aacctggttc attacaaaat tgtaatgctg ctgtaaggtt
     2821 accctggacg ttttttcgct atcctcaaaa ctcattcaca tgacaaggat ataaacatgt
     2881 taaagcgtta tttagtactc tcggtagcaa cggcagcatt ttcattacct tctttggttt
     2941 atgccgcaca acaaaacatt cttagcgtgc acattttgaa ccagcaaaca ggaaaacccg
     3001 ctgccgacgt gacagtcact cttgaaaaga aagcggataa cggctggtta caacttaata
     3061 ccgccaaaac agataaggat ggacgaatta aggcactgtg gcccgagcaa actgcgacta
     3121 ctggcgatta ccgtgtcgta tttaaaaccg gggactattt caagaaacaa aatcttgaaa
     3181 gtttcttccc tgagattccc gttgaatttc atattaataa agtgaacgag cattatcatg
     3241 tgcctttgtt actcagccaa tatgggtatt caacctatcg tggcagttaa tgcgtaaata
     3301 tgtaaaagct gatctctgct actgttaggg aggtgactcc ctgacagatt aacagtaaac
     3361 agctcttgtt ggttaacgac aaaaaagtgt gatggcttat gaaaaagaat caatttttaa
     3421 aagaatcaga tgttacggcc gagtcggtat tctttatgaa gcgtcggcag gtgttaaaag
     3481 cactgggcat cagcgcagct gcattttctt tgcctcacgc tgcgcatgcc gatctgctta
     3541 gctggtttaa agggaacgat cgtccgcccg cccccgccgg aaaaccgctg gagttcagca
     3601 agcctgccgc ctagcaaaat aacctgccac tgacgccagc agataaagtc tccggttata
     3661 acaacttcta tgaattcggg ctggataaag acgatcccgc ggctaatgct ggtagcctga
     3721 aaaccgatcc atggacactg aaaatcagcg gcgaagtggc aaaaccattg accctcgatc
     3781 atgatgattt aacccgtcgc ttcccgctgg aagagcgtat ttatcgtatg cgctgcgtgg
     3841 aagcgtggtc gatggtggtg ccgtggattg gttttccgct acacaaattg ctggcgcttg
     3901 ccgaacccac cagcaatgcg aagtatgtcg ctttcgaaac gatttatgcg ccggaacaga
     3961 tgcctggcca gcaggaccgc tttatcggcg gcgggctgaa atatccttat gtcgaaggat
     4021 tgcgtctcga cgaagcaatg catccgctca cactgatgac cgtaggtgtt tatggcaagg
     4081 cgttaccacc gcaaaatggc gcgccggtgc gactgattgt gccgtggaaa tatggcttta
     4141 aagggattaa atcgatcgtc agtattaagc tgacccgcga gcgtccgcca accacctgga
     4201 atctggcagc gcctgacgaa tacggttttt acgccaacgt taatccgcat gttgatcacc
     4261 cgcgctggtc acaggctacc gaacgattta ttggttcagg cggcattctc gatgttcagc
     4321 gccagccaac actactgttt aatggttacg ccgaccaggt ggcatcgctg tatcgtggcc
     4381 tggatttgcg ggagaatttc taaatgcgtc tgacagcaaa acaggtgata tggctgaaag
     4441 tttgcctgca tcttgccgga ttgttgccgt ttctctggct ggtctgggcg atcaatcacg
     4501 gtggactggg tgccgatccg gtgaaagata ttcagcattt taccggtcgc actgcactga
     4561 aatttttgct tgcggccttg ttaatcacac ctctggcacg ctacgcaaaa cagccgttat
     4621 tgatacgcac tcgccgcctg ttaggcttat ggtgctttgc ctgggcgaca ctgcatttaa
     4681 ccagttacgc attgctggag ttgggcgtga ataatctggc gttattaggt aaggagttaa
     4741 ttacccggcc ttatttaacg ttaggaatta tcagctgggt tattttgctt gctttagcgt
     4801 tcacttcaac ccagtcgatg cagcgaaaac tcggcaaaca ctggcaacag ttgcataact
     4861 tcgtctatct tgtcgcgatc ctggccccaa tacattatct gtggtcagtg aagattattt
     4921 caccgcagcc actcatctac gctgggctgg ctgtactgct ttagccttac ggtataagaa
     4981 gttgctttcc cttttcaacc ggttacgcaa gcaggcccac aacaaattat ctctataact
     5041 tcatattgcc gacaaacttc gcgttatccc ggccggatgc ggcgcgagcg ccctatccgg
     5101 cccacacaga tcgcatcaag ttatgtttcg tttgtcatca acctttcatc accagaataa
     5161 tttattttat ttcaataaga tagttcaata cacctgagaa taccatgctc tcatttccta
     5221 atgagttgtt gcattttttc atatgttaca atataacatt acacataatg tacattaact
     5281 ctggaggaaa ttgttttggc gattcgtctt cataaactgg ctgttgcttt aggtgtcttt
     5341 attgttagcg ctcctgcctt ttcgcatggt catcattctc atggcaaacc cttaacagag
     5401 gtcgaacaaa aagctgctaa tggtgttttt gatgatacca atgtacaaaa ccgaacgctc
     5461 agtgactggg atggagtctg gcaatccgtt taccctttac tgcaaagtgg caaacttgat
     5521 cccgtctttc agaagaaagc ggatgcagat aaaaccaaaa catttgctga aattaaagat
     5581 tattatcgca aaggttatcc gacagatatc gaaatgatta gcattgagga cggcattgtc
     5641 gaattccata gaaataatga aacaacatcc tgtaaatatg attacgatgg atacaaaata
     5701 ctcacctata aatcaggcaa gaaaggcgtt cgttacttat tcgaatgtaa agatcctgaa
     5761 agcaaagccc ctaaatatat acaatttagc gatcatataa ttgcaccacg aaaatcatct
     5821 cattttcaca tttttatggg taatgattcc cagcaatctt tattgaatga aatggaaaac
     5881 tggccaacgt attatcccta tcagttgagt agcgaagaag tggtcgagga gatgatgtct
     5941 cattgatttt tttgcgctga cttcagttat ttaacagaac agtgcctggt agtaacaaca
     6001 ataatttacc aagacaaata agtaagacaa actcagacac gctcatactt gagtcgtgtc
     6061 ttttttatta tgtctgtaga aaaaaactga gaaaaaggcg aacaataaat acacaactca
     6121 gccatacaga ctggcagcgc aaatgtttcc aggagcaagt catctgtcat ggtaaagcta
     6181 accagaaata taggttaatt ttgttgttta aacctttttt acttgttagt ataatacaaa
     6241 ggctttcaaa aaagctggga aattcaacaa ctcttggaag ttatggactt tgtcccgcgg
     6301 ctttaatctg acggccgcgt tccttttttg gctcaattat tgaccccttc tctacgcaac
     6361 ttcagttccc gccagcaact ttgcggcagt ttcataggat caatgtctaa aagaataatg
     6421 gtgaccgata agaaaacgac tgaataactg tagattttcg ctcgaaacct tcctgtcagc
     6481 tccatagcga atcaagcgct gaatgtcaca gtatcgaaca gaaaacagtg acgatctaac
     6541 ccttcaagaa tattctacga ttgttctgtt taggaaaagc aaggcgggaa gtcgggagat
     6601 aagtcattga taaagtggcg gagagagggg gatttgaccc cccggtagag ttgcccctac
     6661 tccggttttc gagaccgatg atacagagtc gaaaaatcaa agagttaaaa atttttcttg
     6721 gaaaaaatgt cttaaaatcg tacacgaaaa atcaatgaat tacataacat taaagaagaa
     6781 tattccaagc cttcttgagt ccaccacctg ttttcaggtg tcattcagac gtgatttgcc
     6841 ccagattggg aaagcacgct tttttctcag cttattccgt tttgcctgtg tgttgaataa
     6901 ttccactgca gttatat
//
LOCUS       DAJTNM010000164         6914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTNM010000164 DAJTNM010000000
VERSION     DAJTNM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.164"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..210)
                     /locus_tag="OO172_003741"
     CDS             complement(<1..210)
                     /locus_tag="OO172_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR5513746.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINAS"
     gene            complement(207..2591)
                     /locus_tag="OO172_003742"
     CDS             complement(207..2591)
                     /locus_tag="OO172_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCR5513747.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            complement(2809..3681)
                     /locus_tag="OO172_003743"
     CDS             complement(2809..3681)
                     /locus_tag="OO172_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5513748.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHWGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            4012..4194
                     /locus_tag="OO172_003744"
     CDS             4012..4194
                     /locus_tag="OO172_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513749.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNSEDN
                     DEAASDYALKINESSD"
     gene            complement(4494..4919)
                     /locus_tag="OO172_003745"
     CDS             complement(4494..4919)
                     /locus_tag="OO172_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5513750.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPGEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMMLENPSPELLTVLIRELSGRGR"
     gene            complement(6037..6549)
                     /gene="clbS"
                     /locus_tag="OO172_003746"
     CDS             complement(6037..6549)
                     /gene="clbS"
                     /locus_tag="OO172_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin self-protection protein ClbS"
                     /protein_id="HCR5513751.1"
                     /translation="MAVPSSKEELIKAINSNFSLLNKKLESITPQLAFEPLLEGHAKG
                     TTISVANLVSYLIGWGELVLHWHDQEAKGKTIIFPEEGFKWNELGRLAQKFYRDYEDI
                     TEYEVLLARLKENKQQLVALIERFSNDKLYGKPWYNKWTRGRMIQFNTASPYKNASGR
                     LNKLQKCLAE"
     gene            complement(6584..6868)
                     /gene="clbQ"
                     /locus_tag="OO172_003747"
                     /pseudo
     CDS             complement(6584..6868)
                     /gene="clbQ"
                     /locus_tag="OO172_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis thioesterase ClbQ"
BASE COUNT         1728 a         1738 c         1576 g         1872 t
ORIGIN      
        1 cgaagcattg atggcattct gccgtgcagt accttcgccg ctcagcccct cttttaaccg
       61 ccggaagaac gtgcgctctc cggccgtacg gatatgctcc tgtgcaacat caataccgtt
      121 tgcgaaatca accacaaatt tttcaggaag acaggtgagt acctgacata cccggttatc
      181 agcaatttgt tgaataaatg gtgaagtcac agcattgtct ccagttcttc ccttaacgcc
      241 ttactgtcag taatgagctc aggaaccctt tcatttaacg cctgaagacg cccggtcagc
      301 cgttcctgcg cctgctgatc gcgtttatga tcttcagagc tctgaatcag cgtgttacgc
      361 agctgttcaa ctttctccct gaatgtacag aaaaaggcat cgatttcctg gcggacgggc
      421 tggttaatat cctgctcaat ggatgcctgc agctccccga aatatgcccg ggtcagtgac
      481 atcacttcct ctctgacctt gttgatattg atcacgctgc ggctcacatc cacactgtac
      541 tctttccagc cccagtcact ggtgtttgcc catcgacaaa gcgtacccca gacaccatcc
      601 tgtcgacagc gacgggttac tgtctccgtt ctttcctcca taagcgcgct cagatttgtt
      661 tttactgcga gccgggtctg taactggctg actgccggaa aactgatttt tacagtgaaa
      721 ccttcatcct ccagacgggc attgatgcgt ccggcaatat tttctgtggc gtacagggcc
      781 gtaccacgaa aacctgtttc aatacccctg acgatttttt ccagttcagg acggaactgt
      841 gcttcagcct cacggtgcag gctggtcacg gtcgattcga tttgcgtcat cagttcaagg
      901 gcttccctgc gatcactgaa ttttatttcg gggctgtccg gatcaaagtc ccgcatcctg
      961 tcatgctgat ttccggttcc gaaaatatcg tgaaagaaag cgaatgcgtt tctctcccgg
     1021 ggctgcgctg aattttcttc ctccagcatt tgctgttctt tgcgtttacc gcttctgaaa
     1081 tagtcattca gctcctcttc cagtgaggtg cagacccccg tcagtaattc ccgggttctg
     1141 gcatcgatct cctccatggc cattctgaca tcagcggtca cctgctcctg acagtccgcg
     1201 atttcccgga tatccgcaag caggctggtg atatgcgact gcagtgactg aatgctttgc
     1261 tgtagcccct gatggcgtaa tgacagatat tcgctgacat tttccgcatt ctgcatcagt
     1321 tttgcggcgg cagaatcaac cgccagtgcc gccgctctgg aatgggagct ctgtatgact
     1381 tccgttatca gttgatcgat aagtgagcct tcccacaggt ctgtcgcccc ttcggttgcc
     1441 atttcactgt ctttacagac gtattctttt ttcatgcgac cgaaggcttc cctgacaaaa
     1501 tcatcgaccc aggcttcatc aacaggaagg gttctgtttt tccgtaactc atgaagcgca
     1561 cggttagcca ggtacgcctg acgtgatgag cctggataaa cgcgggaggc gggcagcaca
     1621 tcgctgttca gcattgccgg aactttctgg cgtacggcat ctgccccatc gccattgcgg
     1681 tctttctcat caaatttatt gaccagcaca aacaggcggc cggctgatac gtcagcaatg
     1741 gcattaagct ctttacggac gtcttcatcc gcttttgagt tcatctgggt gtaatccatc
     1801 accgccagca ccgcagaggc tttctgcagt tgatcccgca tcatcacttc catctgcggt
     1861 tgtccggcct cattagggcc gggggtatcc agcagtgtga gtgttccctg gcatgcatcc
     1921 atccccacca gatggctgaa ttccacgtcg atcaccggca gtttctgcac ttctgcgtat
     1981 tcatcaaaag gaaactcact ccccattgcc gcagcaaggc gaaccagatc gtttaacgat
     2041 gccagtccgg taaagatttc gtcttccccg tggtattcat ttttgagcca gccatcattt
     2101 gccagaatat ccagcagttc gcaggtatcc ccggtttgct gcagggtctg tgcgacttgc
     2161 tgtcctgccg gggtgtcgag tttttgttgc agtgtgctta ataaattgcg gacgggctga
     2221 atatgttcca gatggaggac cggctcagtt tttccgggaa cgtggcggat gagcgtcggt
     2281 acagaggtca tggggcggtt acggttaggc agaatttcct gccccacaat ggcgttgatg
     2341 gtagttgatt tacctgcttt catcgtgccg acaacagcca gcaccatctc cctgcgggcg
     2401 gttttgattt gttcgccctc cagctcctca attcttttca gtgctttctg tttatcaaaa
     2461 agcagtctgt tttcgttctt actgtcagat aacacatccg gttcatcaac catttgccgg
     2521 agcagattaa tgttcagttg caaaagtcgg tcggcttcat cacaaagcag ggcgatgttc
     2581 ttttcgtgca ttatttaacc ttcctgagac gcattttcat gacaaccacg gaggaggtca
     2641 tttaccacaa acgatcgttt atagcgatcg atatgatagt aacgatcgat gcattctatt
     2701 aacttgagaa acacaacgca aggggacaac ccgggtatgg ctgtgtttgc attgtgtttt
     2761 catggggtta aaaatctgta acaagagtct ggtgcatcac caccgggttc agaagaaaat
     2821 ccagttccat accgcgcggg caacagagac cacggtgtca cgaacagcgc gcagaacctt
     2881 acgggcaaca gaggcaacac agacgctctc cgccgtgtca aatatccggt ccaccgcacc
     2941 ggtaaactgt tcacgggcct gaccccggac agattccgtg cgcagcccgt cctgcaggcg
     3001 ggtcatcagg ggactggcgg catggtcggg aagcgctgtc atgagcgcac tgaccagcgt
     3061 atccagttcc cagccagtgc gggccgatac ggccacaacc ggatgtacgg gccggaacag
     3121 acggaatacc gcctccgttt tttcgcgaat attctgtgcc tgtgcaggag aaggctgaat
     3181 accggccata tcccattcat ggcagggctc cgttttgtcg gcctgcgtca ccacaaacag
     3241 tacccgctga tgtccccagt gcaggatgtg tcgccagaaa tactcatcca cagacagggc
     3301 acggtcatcg gctttaatca gccacagtac caggtccagt tcaggcagaa tgtcacggta
     3361 cagggcctca tactctgcat ccctgtcccg gctctcgccc accccgggca ggtcagtgat
     3421 aaccatgctg tgaccatggc cactcagacg gaagcgccgt acttcccggg tgccggcgtg
     3481 aacatcactg accggggtga cctccccctg aaacagtgca ttacagagtg aggatttacc
     3541 ggccccgctt ttacccataa tgccaatcac gggttcgtga ctggtgagtt tgcgcagatg
     3601 ttccaggatg tgacgggaaa gtgagtaagg cagggaggag agcggttttt caattgcctc
     3661 aatggcatca gacggattca tacatgtatt cccggtgaaa aaagacaaaa ccccgcgcac
     3721 cggaaaggtg gcgggggact cacaacgata atatctgtct gaaattattt aggatacagg
     3781 tatcttcaga taatcatacc tccagaattt tgttcttaat agttgagtag tggtatttgg
     3841 tttccctgag agaatataaa ggtgaatcat gctgcactga gtttgattct atttgtactg
     3901 agacaagtat cttcatgatt ataatcaaaa gttgggtggg ttggtctggc gctcatcatt
     3961 ttttcgaata aactcactaa ttattgtaac caccccaaag gagatgattt catgtataat
     4021 tgtggtgaat catattcaga atacgacata tcattatctg acgatgaaaa ctatgacgat
     4081 gactattatc gtgaacgccc gtgtccgggg gaagttttcg gctatgataa tagtgaggat
     4141 aatgatgagg cggcatctga ttatgcacta aaaataaatg aaagctctga ttaatacggg
     4201 ggaagctgat agtaagccaa tcaataacag atattaatta atgaatgaat gaatgaatga
     4261 ataaatataa tcaggcgctc aatattttat ttaatcttgg cgcctgatta aaatccgagg
     4321 taacatataa taattctcat caaaatagtg gatttgcccc tatatttctg agtatctgtt
     4381 atcacttaat ccattacaga tcctccttca gcatatgtcg tatccgttga aagatttaca
     4441 catatcggta acaccggcaa cgagccagat acgggtacct gatgggagtg aaatcatctt
     4501 cccctcccgc tcagttcgcg gatcaacacc gtgagcagct ctggcgatgg attttccagc
     4561 atcatgttac cgtgacggaa ctccaccttg caggaactgg cactgactct ggtctgagtg
     4621 gaagtggata aagacggcgc aatggccgcc acaggttctt tctgctcacc cggcgttatt
     4681 tctacaggta ataattcaac gccagtgtca gaagaggtcg ttaccggaag acgccgcgaa
     4741 acacgccctt cgttctgcca gagcctgagc catttgaaaa taacattatc attgacgcca
     4801 ttttcacgtg caatctgtgc aacacaagct ccaggttgtg atgccagttc caccatacga
     4861 agtttgaatt cattcgaata gtttttacga ggttcttttc gccagtcctg taattccata
     4921 cttagatgtc cgtctatatc agatgggcgt ctaagttacc aattctcgtc tgatggctac
     4981 atacggcggt cagtttacgc ttactacaat gatgataatg cgattatata aaggtcaaac
     5041 catatggttg ttagcggatt tcaacaacgg tgctgtactc gctgagcggc acatgaagtt
     5101 tgaataatgt ggaaacaaca aatggctaca ttaagtcatt ggacatatac agagatggtt
     5161 ggcaaatcga gtacacgata tgttgcctga aaagtcgacc gaacagaact ataaatatca
     5221 agtcggcata acacatctaa atttcataag ggaagatgtg ccaccgggga cgtttttttc
     5281 accacgggga cttcgggtat aacgatgcaa tccgaagaaa gaaatccaat gttggcgata
     5341 ttcatcatcg ctttattcgt ccactcatgc gaatggcaat aaagcgactc accaaaacag
     5401 tttctttagc ttccctctac caaaattccc tatgatcgca tgttagaact ctgcgtgctg
     5461 aacttcgggc catatggacg gtatcattca tgcacttgct cctttgaaat tgctggatat
     5521 cattcgtctt tatctgcacc ggccggcaca aatagctgta ggggaacccg gcgcaggcaa
     5581 aaagagcgac tttaacgcgt tttgcaggac tccccgttgc cttgcaggga gacactgtat
     5641 catcaaacac catcttaatc ccagtacatt accctccatc ctggctcgta ccaaatcaga
     5701 ggaaaggcct cgcggcaata gattttcccg gcgaagctcg gagtgaaata cagtgcagca
     5761 atatccgaca tcagaatttg atcctcctgc gtcaggatac gaaaaaacat cccgatttga
     5821 gcacccttgt cgaaaaaaaa tcaccttatt atgaagattg gttcttaata cacatgcgtt
     5881 cgcatgtgct tatgttaagt agacttattg tcttacacat tttatccgta tcattaacca
     5941 gaatatgttc cggagcagcg ggtgatagtg ccgccagtcc acactcatcg ctgcccggaa
     6001 aaatcatcag tggggaggca aacggtaagc accccgctat tctgcaagac atttctgcag
     6061 tttatttaac ctccccgaag catttttata aggcgaggcg gtattaaatt gaatcatacg
     6121 acctcgggtc catttattat accaaggttt accgtaaagc ttgtcgttac tgaatcgttc
     6181 aatcaaagcc acgagttgct gcttattttc ctttaaccgt gccaataaaa cttcgtactc
     6241 tgtaatatcc tcatagtcac ggtagaattt ctgtgctaaa cgccccaatt cattccattt
     6301 aaatccttcc tcaggaaaaa taatagtttt tccttttgcc tcttggtcat gccagtgtaa
     6361 caccagctct ccccagccaa tcagatagga aaccagattc gctacgctaa tcgtagtccc
     6421 cttcgcgtgc ccttccaata gaggttcaaa ggcgagttgg ggcgtaatag attctagctt
     6481 cttatttaat aaagaaaaat tactattaat agctttaatt aactcttctt ttgatgatgg
     6541 aacagccata tctattgctc cttgtatagt tacacaacta tttctaccct actatttcga
     6601 gtgattcaat cgtctggttc acataaccta ccacctgttc aaaatgctta tcgacaaaaa
     6661 aatgatcggc agcaggaaat ataatagtcc gcgtctttcg tgtggtgaat ttttcccatg
     6721 caagtaattc atcctgcatt accagattgt cagcatcgcc atgaaatagc acgatcggac
     6781 aggttaatgt gcgcgccttg gcctgaaata catactgctc atagagccga taatcgtttt
     6841 taatgatggg ggtgaaaatt gtcattaatg aaccgccccg ggtttcctgg agagtgtttt
     6901 atctgtgaac tcag
//
LOCUS       DAJTNM010000165         6792 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTNM010000165 DAJTNM010000000
VERSION     DAJTNM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.165"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            84..1508
                     /gene="sbcB"
                     /locus_tag="OO172_003748"
     CDS             84..1508
                     /gene="sbcB"
                     /locus_tag="OO172_003748"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5513752.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1551..1778)
                     /locus_tag="OO172_003749"
     CDS             complement(1551..1778)
                     /locus_tag="OO172_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5513753.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1792..2850)
                     /gene="yeeE"
                     /locus_tag="OO172_003750"
     CDS             complement(1792..2850)
                     /gene="yeeE"
                     /locus_tag="OO172_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5513754.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALILAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3029..4387)
                     /gene="plaP"
                     /locus_tag="OO172_003751"
     CDS             complement(3029..4387)
                     /gene="plaP"
                     /locus_tag="OO172_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5513755.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4377..4457)
                     /gene="yoeI"
                     /locus_tag="OO172_003752"
     CDS             complement(4377..4457)
                     /gene="yoeI"
                     /locus_tag="OO172_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5513756.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(4655..5584)
                     /locus_tag="OO172_003753"
     CDS             complement(4655..5584)
                     /locus_tag="OO172_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5513757.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(5630..6454)
                     /locus_tag="OO172_003754"
     CDS             complement(5630..6454)
                     /locus_tag="OO172_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5513758.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6537..6791)
                     /gene="yoeB"
                     /locus_tag="OO172_003755"
     CDS             complement(6537..6791)
                     /gene="yoeB"
                     /locus_tag="OO172_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5513759.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1837 a         1781 c         1593 g         1581 t
ORIGIN      
        1 tcgtagacat actcaggtga aaaaagggtt gcttttcgag ttataacaga cagttaatta
       61 actacagtgg acaatgctag acaatgacag acacagataa acaacctacc ttcctctttc
      121 acgattacga aacctttggc acgcaccccg cgttagatcg ccctgcacag ttcgcagcca
      181 ttcgcaccga tgacgaattc aatgtcatcg gcgaacccga agtcttttac tgcaagcccg
      241 cggatgacta tttaccccgg cctggagcag tattaattac cggtattacc ccgcaggagg
      301 cacgggcgaa aggagaaaac gaagccgcgt ttgccgcccg tattcactcg ctttttaccg
      361 taccgaagac ctgtattctg ggctacaaca atgtgcgttt cgacgacgaa gtcacacgca
      421 acgtttttta tcgtaatttc tacgatcctt acgcctggag ctggcagcat gataactcgc
      481 gctgggattt actggatgtt atgcgtgcct gttatgccct gcgcccggaa ggaataaact
      541 ggcctgaaaa tgatgacggt ctaccgagct ttcgccttga gcatttaacc aaagcgaatg
      601 gtattgaaca tagcaacgcc cacgatgcga tggctgatgt gtacgccact attgcgatgg
      661 cgaaactggt aaaaacgcgt cagccacgcc tgtttgatta tctctttacc catcgtaata
      721 aacacaaact gatggcgttg attgatgttc cgcagatgaa acccctggct cacgtttccg
      781 gaatgtttgg ggcatggcgt ggcaatacca gctgggtggc accgctggcg tggcatcctg
      841 aaaatcgcaa tgccgtaatt atggcggatt tggcaggaga catttcgcca ttactggagc
      901 tggatatcga cacattgcgc gagcgtttat ataccgcaaa agccgatctt ggcgataacg
      961 ccgccgttcc ggttaagctg gtgcatatca ataaatgtcc ggtactggcc caggcgaata
     1021 cgctacgccc ggaagatgcc gaccgactgg gaattaatcg tcagcattgc ctcgataacc
     1081 taaaaattct gcgtgaaaat ccgcaagtgc gtgaaaaagt ggtagcgata ttcgcggaag
     1141 ccgaaccgtt tacaccttca gataatgtgg atgcacagct ttataatggc tttttcagtg
     1201 acgccgatcg tgcagcaatg aaaattgtgc tggaaaccga gccgcgtaat ttaccggcac
     1261 tggatatcac ttttgttgat aaacgaattg aaaagttgtt gttcaattat cgggcacgca
     1321 acttcccggg gacgctggat tatgccgagc agcaacgctg gcaggagcac cgccaccagg
     1381 tcttcacgcc agagtttttg cagggttatg ctgatgaatt gcagatgctg gtacaacaat
     1441 atgccgatga caaagagaaa gtggcgctgt taaaagcact ttggcagtac gcggaagaga
     1501 tcgtctaatt tgaagccgcc gccgctggta cggcggagga caatgttgag tcaggctttt
     1561 tgaacggtga tgctccacgc tgcatcgcca atctgctgat aatcggtgat ggcatgtcct
     1621 tcttctgccg cccactgtgg gattgcttca gttgcctggg tacaatcaaa ctcaattacc
     1681 aactcatcgc cgctggccat ttctgccagt gctgcttttg cttctatgag tggaaacgga
     1741 cagacctgcg tcaccacatc cagcttttta atcgccatct ttacacctta cttaatttgc
     1801 cgcagcagtt gccagtcgcg ccttacgctg cggtcgaaca tacacaagcc aggacgcagt
     1861 ccagactccg agaatcataa atacaaggcc aatccagccc tgccaggtca tcattgcagt
     1921 cataaccagt ccattaccga tagagcaacc acctgcaata ctggcaccga agcccatcag
     1981 tacaccacct aacccgctac gtaatgttgt ttgtgcatca gctgcgcgaa cgcggaactc
     2041 acggctcgct ttggctgcaa taaatgcccc cacgaagatc cctaacacca ggaaaacgcc
     2101 ccagttaatg tatttcacgt cacccgcgac cagaaattgc agaatattgg ccgttgggga
     2161 agtgactcct aacccaaaca tgcgtccggt tgcttcactc aggggccagg ctaaaagcgc
     2221 aatcaaaccg atgagtacag cagtgacaaa gggatgccag cgtttttcaa acagaatatg
     2281 agcgatcccg gttcggcgcg gcggtaaggt cgcgactttg agttttggct tcttcaactc
     2341 tttcatcacc acccagagcg ttatcaccag caaaacggta accaacggcc acacagacaa
     2401 attaaaagtc tcagcaatag agttatgttc agtagtgtag tgctgcaagg tttgatttaa
     2461 accactggcg tgtggagaac gcatcaccgc actcatcacc atataagtga aaagcgcgat
     2521 ccaactgccg atcaatccct cacccgcgcg ataccaggtc ccggtggcac atccacccgc
     2581 cagaacaatt cccagcccga aaagataccc acctataaca gtaccaagcc acgggaacgc
     2641 cccggcttcg taagtcagta ggcccgccag aattaacgca aaaaccccca cgctttgtgc
     2701 cgaaattgca atcagcaagg cgtaaaacat gcgattattt ttcgcgatat acatatcgcg
     2761 aaaaccacca gtcaggcaaa aacgcccacg ctgcatgaca aatcccagca gagcaccaca
     2821 aattagcccg cttaatatca ttgaaaacat aaattaatta accagatgaa tgttaatggg
     2881 gaaatttttc atgactggtg gaatgcagac caataaccaa attctctaaa ttagaacaaa
     2941 tggttattaa tgaggatttc gataaaaaag aataaaaaaa ccggagccaa atgttcattc
     3001 gactccggtt tattattaga aaagatgatt atgctacgtc ttcgtactgc ggaaccggat
     3061 tacggaagct cttggtgacg caagccaggt aaatcagacc gatagcagcc cagatcagac
     3121 ccagaaccat tgagctttct tccaggttaa cccacagcgc accaacggtc agcgcaccac
     3181 acatcggcag gaacagatac tggaagtgat ctttcagcgt cttgttacgc ttctcacgga
     3241 tccagaactg cgagatgacc gacaggttaa caaaggtgaa cgccaccagc gcaccaaagt
     3301 taatcagcgc cgttgccatt accaggtcga agttgattgc cagcagggca atcgcgccaa
     3361 ccaggatgat gttcatcgcc ggagtacgcc atttcgggtg tacataaccg aagaagcttt
     3421 tcgggaacac gccgtcacga cccattacgt acatcagacg cgctacgcct gcgtgcgccg
     3481 ccataccgga cgccagtacg gtaatggtgg agaagatcag cgcgccaacc tggaacgctt
     3541 tacccgcaac gtacagcatg atttcaggct gtgacgcatc cggatcttta aagcgagaga
     3601 tatccgggaa gtacagctgc aggaagtaag ttgcaaagat gaagatcatg ccgccaatca
     3661 gcgcggtcag gaaaatcgca cgcgggatca cgcgctctgc atctttggtt tcttccgaca
     3721 gattgctgat gccgtcaaag ccggtaaagg agaagcacag gattgtcgcc ccggtaatca
     3781 tcgggataac atgcgcatcg ccagaccaga acggacgcgt gctcgccagc gtaccagcgc
     3841 cttcaccttc aaatacgcca taaacaacca tgcccagaat caccgcgatc agcactacct
     3901 gcaacacgac gattacagtg ttgaagttcg ctacggattt cagactacgc aggttaaagg
     3961 cggtcataaa ggccaccagc gccaccacaa acatccacga tggaatggaa ggcaccagag
     4021 cttcaaaata gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca
     4081 gagaagacca acccaccata aagccgacag tcgggctaat ggatttctgg gcgtaagtgt
     4141 atgcagagcc agcagaagga tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca
     4201 ggatcgcaat caatgcgaac gcataggctg tcggcacatg accatccgtg aggcctgaaa
     4261 cgataccaaa tgtatcaaac agcgtcatcg gctgcatata ggctagaccc atcattacaa
     4321 ccggaactaa cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat
     4381 gcgacatggt tattctcctt tacggtgata accgtcgcgt aagcaaaaaa tttgccccat
     4441 ttttttggat tcctcagcga caacaactgt cgatttttag taaatatcta tccggtacga
     4501 agcccggcct cttggtatga atgattggtt tgaagcaaaa aataaccgac gctaattaaa
     4561 cgtcggtttt tagtcatttt ttgacagcgg cgcattgtgc ctaaatgggg gggaaatgac
     4621 aagagaatga gaggcttgtc ataataattt ttctttaaat ggctgatttt ccgtcatcag
     4681 attttgcata aacaccggcg atcgcactat ttgctaaaat ttcatctcgc caccagccgc
     4741 tggcaagccc tgcggtctgt tcattccagc caatccagac aggttcaaag agagtttggg
     4801 cgaccacttt cttctcgatt aacgcgccac tatcgagaaa acgttgcgcc agataccgtg
     4861 gtaaatagcc gcagccaagg ccgctaattt gcagctccag tttggtttta aaatcgaaaa
     4921 cggtaatggc ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct gcctgcgcag
     4981 tgtcccccac cacaatcgcc cgatatcgtt taatgatacg acggtttaac ggttcttctt
     5041 cctgggctaa cggatgatga ggcgcgaccg caaagacttg ttcaagatcg cccaaccgcg
     5101 agaaaccaaa ctcactggaa gatggtggct catgcatcgc gccaacgata atatccgctc
     5161 gcccctgagt cagggcatcc caggaaccac cgagtacgcc attgataaac ttcaggcgcg
     5221 tcacgctatg atgttgataa aaagcttcaa tgagaggcgc aagcagagaa aaaggaaacg
     5281 tgtcgtccac gccaatcacc agctcgtttt cccagccttc atgcagtttg atcgcctgct
     5341 tttccagttc tcgcacggta tgcagaactt cccgcccttt ctctaataac atttttccgg
     5401 tgcgggtgaa tttagcgcgg tgtccgctac gatcaagcaa ttgaatattt aggtcgcttt
     5461 ccagcttgtg aacggtgtaa ctcagagcgg acggcgtctt gtagagtttg gctgacgccg
     5521 ccgcaaaact gccctctttt tctatggcat cgaggatcat caacacatcc agcagtggtt
     5581 tcatacacgc ccccttgcag tgcatgcggc gtcctgctgg cgtgatcgtt tactccagcg
     5641 gcattaccag cggatcggga tactggtatt caaatcccag ttcattacaa atccgactgc
     5701 catcaataat cttacctttg ccgctgtcca gactatttct gaactgcgga ggttgcagcc
     5761 ccagtaaacg ggccatctgc ggatagaaaa cattacgcgc agggtgagct ggcgcacata
     5821 tattatagat gtgtccgcct ttaggtgcct gtaacaacag agtgatagcg ccaataacat
     5881 cttctaaatg gactaaatta acaccatgtt caccatcagg ggcggttttt ccggcaaaga
     5941 agcgtcccgg atgacgtccc ggtccgacca ggcccgcaag acgcagaata tcgaccgaag
     6001 taccgggtaa attgtgcagc cagtcttcga gttcttctaa tactcgtcca ctgttggtta
     6061 ccggattacg cggggtagtt tctttaaccg tgccttgcgc gtcgccatag acagatgttg
     6121 agctggtaaa aataattcgg ggaatacgat gggccagcgc gctatccact aactcttgta
     6181 ccgcttgtaa atagaactca tcgccggggc cgctacgacg tgccggaagc gtaatgacca
     6241 gcgcatcggc atccataagg gcatccagat cgtcagaatc gcaaactaac tcaggctcca
     6301 tgcgaagcag atagctatca atgccactca ttcgggccgc ttcgacacca tcttgtgtgg
     6361 ttttactccc ggtgacttgc cagcctcgcg ctgaaagtga catcgccagc ggcataccta
     6421 accaccctaa cccgacaatt gcgacctttt tcatccgtta tctcctgata caccttagat
     6481 ctataaggct acgctagcgt atcaaaactg acaattcatt ctatgaatga atctgttcaa
     6541 taatgataac gacatgctgc aatgagcagt gaatcatcag taaccgcgta taccagacgg
     6601 tgctcctctg taatgcgtcg ggaccagaaa cctgacaaat tatgtttcag gggttctggt
     6661 ttccccttac cttcaaatgg tgttctgcgg gtatctttga taagttcatt gatcttttta
     6721 acaattcgct tatctgtttc ctgccagtac agataatcat cccatgattc ctcagaccag
     6781 attagtttca ct
//
LOCUS       DAJTNM010000166         6761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTNM010000166 DAJTNM010000000
VERSION     DAJTNM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.166"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..3513
                     /locus_tag="OO172_003756"
                     /pseudo
     CDS             1..3513
                     /locus_tag="OO172_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3523..3766
                     /locus_tag="OO172_003757"
                     /pseudo
     CDS             3523..3766
                     /locus_tag="OO172_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            5305..6761
                     /locus_tag="OO172_003758"
                     /pseudo
     CDS             5305..6761
                     /locus_tag="OO172_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1790 a         1777 c         1627 g         1567 t
ORIGIN      
        1 cgaaagataa ttctttgcaa ccttcccaaa cggtaacgga ctctctcaag gtagcggact
       61 tatccaccat tattcaggaa caaccctctt tcgtagcaga aacccgtgcg gctgacaaaa
      121 atacagatgc tgttttacct tggctggcga aagatattgc tgtcgttttt ccgcccgaag
      181 ttgtacacac cacgttaagt catcgccgct ttcccggagt tcctgttcag caagcagaca
      241 aattgaccca attacgtcgc ttagcctgta gcgtgtcgca acgggataat aaaacagcca
      301 catttgactt cagcacctgt tctttagaat ggcaaaacac cgtcgcccaa gcgatcagtc
      361 agatcgacgg cctgaaaaca acacagctac catcaccagt aatggctgta ctcacggcac
      421 ttgaaatgaa atgcacaaga tataaagtgc gtgaggatgt tatggatcag atcgtccagg
      481 aaggcggtct ggaatatgct actgatgtaa taatacacct tcaacagatt gatattaaat
      541 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
      601 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      661 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      721 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      781 gtctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      841 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      901 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      961 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
     1021 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
     1081 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agaacaggtc attccctggc
     1141 tttcgacacc cgcagttgca gtgctgaaat catgccagca ccaactgaca cagccctcaa
     1201 accatgccag cgccgatcta ctgccagcca tagtagtctc cccaccatgg atttcgaaaa
     1261 agaaaaaatc gccgattccg gtgctggatt tagcgccatt aggcatcgag ccaatctgtt
     1321 atctgacaga agaaatcagt aatcaacttt tggcgaaata tatctggtat tcaaaacaca
     1381 tcacggttag ccatgaagaa agtactacca acctgttggc aaggatgggt tttcaacgac
     1441 ggatcgctgg tacatatatt aaagctcccg aagcggtagt tgaggcatgg ctaaatgaag
     1501 attattcaac cttactaagt gaatttaagg tgtttcattc acctaccggg cattattggc
     1561 agttggggat tttgacaaca ttgccgctgg aggctggaga aagcagtaaa agcatggaat
     1621 gcccttactc tatctccaca taccgatacc gaatacgcca tgttacattt tggactcaaa
     1681 ggtttacctg gtttagttaa ctcaattaca cgctatccgc aggaagcctt gcccatcacg
     1741 aattacttcg cagcgagtga gctggctcct gccgtcgccc gtgccttcaa caaactcaaa
     1801 acgctacgcg aaaacgcccg cacctggctg ttgaaatacc cggaacatgc ccttaccggc
     1861 ctgctgcctg cggcgctcgg caaagccggt gaagcacaag ataacgcccg cgctgccttg
     1921 cgtatgctta ccgaaaacgg tcatcagcca ttactgcaag aaatcgcccg gcgttataac
     1981 cagtcggaag taacagacgc ggtgaacgct ctgcttgcgc tcgatccctt agataatcac
     2041 ccgacaaaaa tccccactct tccgaccttt tatcagccat cgatctggac gcgcccggta
     2101 ttaaaagcaa acgcccaatc actgccagat aacgccctcc tccacctcgg cgaaatgctc
     2161 cgcttccctc aggaagaggc tctgtatccg ggattattgc aggtgaaaga cgcctgtacc
     2221 gctgactctc tggctgaatt tgcctgggat ctgtttaccg cctggcagac cgctggcgcg
     2281 ccgtcgaaag agagttgggc gttcactgcg ttaggcgttc tcggtaacga tgacaccgcc
     2341 cgcaaactga cgccattaat acgcgcctgg cctggtgaat cccagcataa acgcgccacc
     2401 gttgggttgg atattctcgc cgctatcggt agtgatatcg ccctgatgca gcttaacggc
     2461 attgctcaga aactgaaatt caaagcatta caggagcggg caaaagaaaa gattgccgac
     2521 attgccgaga gccgcgaact cacggtagcg gagcttgaag atcggttagc accggatctc
     2581 ggtctggatg ataacggttc gttgctgctg gattttggcc cacgccagtt caccgtcagc
     2641 tttgatgaaa ccttaaaacc gtttgtgcgt gatgtttccg gcagccgcct gaaagacctg
     2701 ccaaaaccaa acaaaagcga tgatgagtca caggcgaacg atgctgttaa ccgctacaaa
     2761 ttgctgaaaa aagatgcgcg taccgtcgcc gcccagcagg tagcaaggct tgaatccgcc
     2821 atgtgcctgc gtcgccgctg gtcgccggaa aacttccaac tcttcctggt tgtgcatccg
     2881 ctggttcgcc acttaacccg ccgtctgatt tggggcgttt atagcgccga caaccagcta
     2941 ctggcctgtt tccgggtagc ggaagataac agctacagca ccgctgacga tgatcttttc
     3001 accctgccgg aaggcgatat ctctatcggc attcctcacg ttctggaaat atcaccgacg
     3061 gatgctgtcg cctttggtca gctttttgcc gactacgaac tgctaccacc gttccgtcag
     3121 cttgaccgta acagttacgc cctgacagaa gccgagcgca acgccagcga actgacccgc
     3181 tgggcaggca gaaaatgccc gagcggccgg gtcatggggc tggcaaataa aggctggata
     3241 aagggcgaac cacaggatgg aggctggatc ggctggatga tcaaacctct cggtcgctgg
     3301 tcgttaatca tggaaatcga cgaaggtttt gcggtgggta tgtcaccagc cgaacttagc
     3361 gctgagcagc tcttaagcaa gctgtggcta tgggaaggca aagcggaaag ctatggctgg
     3421 gggagtaatt caacacagga agcgcagttc tccgtactcg atgccatcac cgccagcgag
     3481 ctaattaacg atattgaagc cctgtttgaa taaggaaaga gcatggacaa ggaattaccg
     3541 tggctggcgg ataacgccca actggaactg aaatataaaa aaggcaaaac accactcagt
     3601 catcgcaatt ggccgggcga accagtgcct gttatcactg aaagcatcat tcagacactg
     3661 ggtgatgaat tgctacaaaa agctgagaag aaaaaaaaca ttgtctggcg ttatgaaaat
     3721 ttttcaccgg agtggcagtc cgccattacg caggccatca acttgatcgg cgaacacaaa
     3781 ccctcagtcc cggcccgcac aatggcggca ctagccttta tcgcgcaaaa tgacaaccaa
     3841 tagttgctcg acgaaatcgt ccaacaagag gggctggaat atgcgactga ggtggtgatt
     3901 gcacgccagt ttattgcacg gtgttatgag agtgatcctc tggtagtcac attgcagtat
     3961 cagaacgagg actatggcta tggttatcgc tcagaaacct ataacgaatt cgatctccga
     4021 ctgcgtaaac acctctctct ggcagaggaa agctgctggc agcgttgcgc cgacaaactc
     4081 attgccgcac tgccaggaat aaccaaagtt cgccgccctt ttattgcgct gatcctcccc
     4141 gaaaaaccag aaatcgccaa tgagttggta agccttgaat gcccgcggac tcattttcat
     4201 tctaaggagt ggttaaaagt tgttgctact gaccccaaag cggtgagaaa actcgaacgc
     4261 tactggagcc aggatatatt tagcgatcga gaagccagct acatgtcgca tgaaaaccac
     4321 ttcggctacg cggcctgcgc tgcccttttg cgcgaacaag gactggcagt cgttccgcgc
     4381 ctcgcgatat atgcccataa agaagattgc ggcagtctgc tggtacaaat taaccatccg
     4441 caagtcatcc gcaccttgct gttagttgct gataaaaaca aacccagcct gcaacgtgtg
     4501 gctaaataca gtaaaaactt cccccatgcg acactcgccg cactggcaga actgctggcg
     4561 ttaaaagaac caccagcccg cccaagttat ccaatcatcg aagacaaaaa gctgcctgca
     4621 cagcaaaaag ctcgagatga atactggcgt acgctgttac aaacgctgat ggcatcgcag
     4681 ccacaactgg cagcagaagt gatgccgtgg ttaagtactc aagcccgggc agtgctgaat
     4741 agttatttat cggcaccgcc caaaccagtt attgatagca ccgataacag ccaaatgcct
     4801 gaaatcctcg tttcaccacc atggcgtggg agaaagaaaa agacttccat acgtcacttt
     4861 tcgctgcctg atcttacgct tgcaccgcaa acgtacaggt cgccgaataa acactggacg
     4921 ccggaacagc aaactgcaat tgaccatttt agcgccatgt cttttgacga acgtcttgct
     4981 acgagaggag cggatatctt cttgcgggaa ttaggttttg acgttcatag cctggaattt
     5041 aataagcgtc ttcttaatgg acagttggat gcctttgatg ctcagcaact gcaagaatac
     5101 acatcaatag gactattgcg tacttatcac gcgcatcagg ccaaatctaa acagtaccgg
     5161 gaagacgcca ccaatgcatt actggcacaa gatagcattg cgttgatggg agggtggcga
     5221 acttttcagc acggatttta ctatggtaat gaacagcgtt gggggatttg gaatctgtac
     5281 cttatcgcgc agatgccgcg cgaaatggcg gtatcgtgct ggcaacaaat tgtcgaggct
     5341 gattttcatt acaccggggt ggaatatttg ttaagtgtgc tgggtaccga tgctttgcct
     5401 ggattaaaca ccgcctttgc ccgacatcca aaagagatat ttccgctgct gattcatttc
     5461 ggcgcaacag aactggccct gcccattgcc cgcgtctggc acctttttgc gggccagcgt
     5521 aatctggctc gccagtggat tttacaatgg ccggaacata cggctactgc acttatccct
     5581 cttgtcttca ccaaatctag cgataaaagc gaagctgcat tacttgccct gcgtttactt
     5641 tacgaacatg ggcatggcga attgctacaa actgtagcca atcgttggca gcgtacagat
     5701 ctatggcctg ccctggaaca attacttaaa cagagtccaa tagagattta cccggcacgc
     5761 attccaaaag cccctgattt ctggcatccg caaatgtggt ccaggccgcg ccttatcact
     5821 aataatcaac ctgttaccga tgacgctctg gaaattatcg gcgaaatgct gcgctttacc
     5881 caggggggac gtttttatag cgggctggaa caactgaaaa cgttctgcca gccacaaacg
     5941 ctggcagctt ttgcctggga tctcttcact gcgtggcaac aagctggtgc ccccgcaaaa
     6001 gacaactggg catttctggc gttaagtctc tttggtgacg aaagcacggc acgggatcta
     6061 acgacacata tcctcgcctg gccacaagaa ggcaaatctg cccgtgcggt cagtggcctg
     6121 aacatcctta ccctgatgaa taatgatatg gcgctgatgc agctgcatca tatatcgcaa
     6181 cgggcgaaat cccgcccctt acgtgataac gcggcggaat ttcttcaggt agtcgcagaa
     6241 aatcgcgggc taagccagga agagttagcg gacagattag tcccaacact gggccttaat
     6301 gatccgcagg cgttgagttt tgattttggt ccccggcagt ttaccgttcg cttcgatgaa
     6361 aacctcaacc cggttatctt tgatcagcaa aacgttcgcc agaaaagtgt tccccggttg
     6421 cgcgccgatg acgatcaact gaaagcgccc gaggcactgg cccgactaaa agggttaaaa
     6481 aaagatgcta ctcaggtgag caaaaacctg ctcccgcgtc ttgaagctgc cctacgtacc
     6541 acccgacgct ggtcgctggc agattttcat tctctgtttg ttaatcatcc ctttacccgt
     6601 ctggttaccc agcgattaat atggggggtt atccggcaaa tgaaccgcgt cgtttactca
     6661 acgcctttcg tgtggccgca gagggggagt tctgcaatgc gcaagatgag ccaattgacc
     6721 tgcctgcgga cgctctgatt ggcattgccc acccgttaga a
//
LOCUS       DAJTNM010000167         6744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTNM010000167 DAJTNM010000000
VERSION     DAJTNM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.167"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            205..281
                     /locus_tag="OO172_003759"
     tRNA            205..281
                     /locus_tag="OO172_003759"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:239..241,aa:Arg,seq:tct)
     gene            complement(327..1088)
                     /gene="envY"
                     /locus_tag="OO172_003760"
     CDS             complement(327..1088)
                     /gene="envY"
                     /locus_tag="OO172_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5513760.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINYYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1271..2161)
                     /locus_tag="OO172_003761"
     CDS             complement(1271..2161)
                     /locus_tag="OO172_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5513761.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2162..5140)
                     /gene="nfrA"
                     /locus_tag="OO172_003762"
     CDS             complement(2162..5140)
                     /gene="nfrA"
                     /locus_tag="OO172_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCR5513762.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDP
                     ALIRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVG
                     RRWTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLS
                     VYSRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTML
                     RASASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANG
                     QTIEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFK
                     AINQRNGERNNAFLTIGVHW"
     gene            complement(5127..>6744)
                     /gene="nrfB"
                     /locus_tag="OO172_003763"
     CDS             complement(5127..>6744)
                     /gene="nrfB"
                     /locus_tag="OO172_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5513763.1"
                     /translation="SMTYIDEFSELHGKDVPVREALAGQVPSAGVGTCFSRRAVTALL
                     ADGNGIAFDVQSLTEDYDIGFRLKEKGMTEIFVRFPVVDEAKEREQRKFLQHARTSNM
                     ICVREYFPDTFSTAVRQKSRWIIGIVFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVS
                     FLAMLVMIQLLLLLAYESLWPDAWHFLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFV
                     TGYYGLTQGLLSVLRLFWGNLINFMANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGD
                     TRSLRPLGQILLENQVITEEQLDTALRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQN
                     GVAWESIDAWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTR
                     KVGRKVRYVIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYV
                     PHQFLFAEILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQR
                     ELQVSMQSLLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1546 a         1769 c         1777 g         1652 t
ORIGIN      
        1 ggcggtaaat tgtactttga tacatgaaaa tacgggtttt cttgattcag acgcgcagcg
       61 gtgtgcgttt gtttgccgct atagcgaaat aaatcagaaa atcagacgcg gtcgttcact
      121 tgttcagcaa ccagaccaaa agccattgac tcagcaaggg ttgaccgtat aattcacgcg
      181 attacaccgc attgcggtat caacgcgccc ttagctcagt tggatagagc aacgaccttc
      241 taagtcgtgg gccgcaggtt cgaatcctgc agggcgcgcc atttcttctt catttatgcc
      301 cgtcttatcc gtttccgctt tgcccttcac cacatcactt tttgtcgctg tttggcgaga
      361 taattcaacg gtgtcaggct gtaaaacgcc ttaaaaacag agataaagta cgacgtgctg
      421 ctatagccac ataattgcgc cacctgagtg atatttttgt tatccatcaa taacatctgt
      481 acggcgtaac gcatacgaca ctctgtgaca atctggctat agctggtatt ttcgtttttt
      541 aatttctttt tgagcaggct ggggcttaaa catagcgaac tggcgacaat tcgcagattc
      601 cagtaatgct gaatatcgct ttgaataatg cggcagacgc tgtcgcggac gctgctgcgt
      661 aagatataca tcagtagggc aataaaccgc gattgctcaa gaaagttaga caatacggta
      721 aaaagtaatg cgcgcgtcaa ctccgcttcg cagggtttgc tgggttgctg cttgctgtgg
      781 ttggcggcta gccggaacac ttccggtgta cggctatgac aagcgataac cggggttgcc
      841 agccgcgacc agggctttac acaggttaat ttcttattta aaaacaacag ataatagttg
      901 atgatgttac ggttgatgtg ggtgattaat acgtgattta acgttgagag ttcaatgacg
      961 ttattgttgc acgcgaaggc ggccaggtag tttttcggaa gggtaaacgt agcatggtta
     1021 ttgacgctta cctctacctc tttttcggtc aggatcacca cgcaaggttc actgctgctc
     1081 aattgcattt cgcactcctc agatatcaga aactccgctc aaaggatcta tgcttcctgc
     1141 atgagtgatc ggcccgttcg ccgataacga tcttctttct ttagcatgct ttttagcaat
     1201 taatcttgat ggaattctga cgagagcgaa agaggtaagc caggtcgtac ccgacttacc
     1261 tggaggggat ttaatactcg agaatgccgt gcgcgacggg caaatagcgc agagagaaat
     1321 agagcatgtc tttaccgcaa gaggaacgtt tcgctaacag cgaggcaatt tctgcgtccg
     1381 gtttcggttt cgctgtaaag gttttgcctt tgccggcgac aaacagttca taaacaatgc
     1441 cgcttgctgg ggcggagctt tggcaatcgg cgctggcctg tagataacgt tcacgctgtt
     1501 cagcggtaag tttatccacg ccgctgccat cctgtaccca gacattaact ccggttgctt
     1561 taacttgttc cagcagttgg cgatagccat cgggcgacat gtttccggcg aaaaaactac
     1621 tgatataaac cggttttgct gaaacatcgc taatcagccg ctgcgcgttg tttaaccatg
     1681 ttagcaaagg ttgacgggcg gcttcgctgc gccagttcag gtcgtcaatt tccgcgctga
     1741 tgtaccagcc atccggcgtt atgccaggcg cggcgctcca taatctggct tgctggagat
     1801 cggcagccag caggcgatta agatagcttt ccagcgctgc agacgactgt ttctggtgca
     1861 taaaaaattc cggatcggcg ttcagcccga caatcagctt caggccagtc tgttgcgcag
     1921 ctgcggcacg cttaaacaat aacgtgcgct gttctggctg ggtaaatgca tcgccgtaac
     1981 gggtccattg caaaaccagg gtatcgaagc cttgaaaacg taactgactc atcagcccct
     2041 gccactgggt atcggtaacc tgactatctc ggttttgtgg ttgccagata atacctttca
     2101 tcgcaaagga aaaagggctg accaaaagca gtgtcagcaa tacgaaaatg aacttacgca
     2161 tttaccagtg cactccaatg gtgagaaacg cgttgttgcg ctctccgtta cgttgattaa
     2221 tcgccttaaa ggtatgttga tactcgacgc cgagactgac tttgtgcggc caggcgtcgt
     2281 agtgcgtctc gccggtccaa atattccagc ggaccccgac accgccaagc tgcgcgccct
     2341 gagtgccttt atcacgatag ccgttgtcct gaacgtgagc gtaaggctca atagtctgtc
     2401 cgttagctac cttctgatgc cagctgacgc gataatctgc cgtccacgcc tgaatatcct
     2461 ggcggatata ttgcgccgca tcgaggtaca ggttttgggc aaaccagcct gaaccgttcg
     2521 ggtgccattc gtcgctgtat ttgccgccat taaagaatga ggcgctggcg cgcagcatgg
     2581 tatcggatgc gccattttgc ccgttcagcg gcaactgctg ttcgacggcg aggaaaaaga
     2641 tctgatcgcg cagcggcttc cagcgcagac cggtgccgga catcggattt ttcaccggca
     2701 tcatcacccc gttttctccg gtatcggcaa agacgcggct ataaactgag agcaggtcgc
     2761 cttccagcag catattgcgt ccgatgcggt actcggcttc cagttgtccg tagctacgat
     2821 agcttttccc aggcgctgca ccgccgacat tattgttagc ggtactcatt gcgccggaac
     2881 gcaagccgat ggaagaatcg aaactgaacg tccagcggcg accgacctcc tcatgcaaac
     2941 ggcggaaatt gaagcgttgc tgattttgct ctggggtcag tggggttatc agcgcctgat
     3001 tatcaatgtc atcaatcacc agccgggcgt agtgctgcgt cgcaggcatg tcatccagac
     3061 gctggttcac gtaggccagt tgtcggatca gtgccggatc gtccggaagc cctttatgcg
     3121 cctgttcgag catttcccgc gactgtgcga tatcaccgct atcccacaag gcgtaaccaa
     3181 gcgctgcctg ggtgttgcta ttattcggtt ccagttccag ttccagcgcg gcgcgcaaat
     3241 cactcaccgc ggccggaaca ttatgacgtt ggcgataaat tgtcgcccgc gcaacgtaag
     3301 cgttggcaga aggggcaata ttgattgagc gcgtgagatc gttcagtgcg agttctggct
     3361 gagctgggat ataacgttgc gcatgcagcc accagtattg agcattgttt cccagctcac
     3421 gttgttctgc ctgttgaagc cagcgatcgc gagccgcgcc atttcccgct gcctgagcgg
     3481 tgttggcggc ggcgagcaga tcctcattgc tcatgtcgtg aagactgatt ttctgccagg
     3541 ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg ataggctacc gcacgatgtt
     3601 gccaggcgct cggttgtcgt tgttcggcct gaagccatgc atacaacgcc acaccgggta
     3661 gcgtgtcccg ataacacttt gccagacggt tccaggcggc agcatcgtag gaaggcgaca
     3721 tatcgcccag caagcgaact attgccgggc aattatctgc aatacccggc aactgacttt
     3781 gccactgacg ttgctccgcc agcggtaagg gtttcgataa aatcgccacc ttcgccggcg
     3841 ttgctaggta aggatgactt tccagcagag acgccagtcg cgccattaaa gtctggctga
     3901 cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa atcggcagct tcgcgtgact
     3961 gctcgttctg catcagttgc caggtcagtt gatccaggcg gggaagattt gtcggttctt
     4021 gctgatacag caatcgtgcc agacgcagag attcagcctt gttacgggtc gccacgctga
     4081 cagcataacg ctcctcaagc atttcattgg cggggagggt ggcgagcagt ttttgcgctg
     4141 cgtcgtactg accctctttt aacagcaccg gtagcgtcgc gccaacaaca tactggcggt
     4201 tatcggcaaa ctgcaccgta taattcgcca atgcctgaac ggggttggcg ctgtatttag
     4261 ataacagata gagccaactt ttctcttgtg cgcccgtggt aaacagcggc ttattttcaa
     4321 tgagataatg ctggaggcgt gctttttcgc cacgataagc cagcgcggtc gcgtaagtaa
     4381 tatatgactg aggatcggtg aagatcccct gtgattgcag tgccaggatc cgatcgtcca
     4441 gctgcccggc aagaagcacg tcaaaccact gacggcgttc tgccgcgctt aatgtgttct
     4501 gctggcgtgc ttcattgtat agcgtatctg cctgggacca ttgtttcagg tagattgccc
     4561 gttgcagcag atcggttcgc agcgtttttc cttccggcga tgcagcaaac gtcgcatcgt
     4621 tcagttgcgc tctggcgaca ggtaattgtg ccagccgcag ggcattctgc ccgacttcac
     4681 tgcgacaacg cagggtcggc gcagcgtcgc ccgctttttg ctgggcaagc agttcttcaa
     4741 cagtcgtaac gcttttcact tcaactggaa tagccgccag actgcgctca agtcgggcat
     4801 ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg cgcccggtca tcatgaccaa
     4861 aatgacgata ggcttccgca aggtataaag tcagcggaat attatccggc acctgttggt
     4921 gtatatattc aaattcgcgg atggcggttg cttcgtcgtt atttttctgt gccttcagcg
     4981 ccttatcgag acggggataa ataacaaaat ggcgataatc gctcagcccc agctcttctg
     5041 cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa taaagacgtc agcagtaaac
     5101 cagaccatcc gatgacgcga ttaaggttat tctccttcat tttcggactc cagttgcgca
     5161 acctgttctg tgtttaaacc tgctttgagt aatagtgatt gcatcgaaac ttgtaattcg
     5221 cgttgaattg tcaggacgcg atccaacgtt tcctggctga taacgccttc ggtgaccaaa
     5281 aacttgccga gcggcagaga actgcgttca tggcgcaata acaacacgtt aattgctgaa
     5341 cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca ggaactgatg cggcacatat
     5401 tgccgccaga tttcaccggt ctgctgttcc gtgagccact gatgctgaac cgcattgtac
     5461 aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc agtggcgtaa ccctgtgaca
     5521 atttgtcccc gcagaacaat gacgtaacgc actttgcgtc cgactttacg cgtcagggcc
     5581 gccagcgaaa ccgggtcaat accatcttca ctgccgacaa ttaactcatc attttccaga
     5641 cgcagcggca gtactgcata atgcagcgct acggaggctg gcatttcggc aatcagcgag
     5701 gacgggatct gccaggcatc gatggattcc cacgccacgc cgttttgctc tgcctgcgcc
     5761 tgtgccagct gctcggcgct aatcagcccc tgcatcagca ttgaaccgcc caggcgtaga
     5821 ccttcgacgc gattacgcag tgctgtatcg agttgttctt cagtgatgac ctgattttcc
     5881 agcagaattt gacctaacgg gcgcaacgag cgggtatcgc cagtgacgct ggggaagtca
     5941 tgcgttgttt tatcccacgc cacgcgacgc ggatcgccgt gctgaagaac ctgtttcagc
     6001 acgcgccagt tagccataaa gttaatcagg ttgccccaga aaagacgcag gacggaaagc
     6061 aggccctgcg tgaggccgta gtagccagtg acgaaaatca ctcgctgcac gatgcggtta
     6121 accatcaagc caaagtttag ccacagcagg gtcattaacc atgcgctgcc gctaaaaata
     6181 gaaaggaaat gccaggcatc gggccacaaa ctttcatacg ccagcaacag caaaagctgg
     6241 atcatcacca gcatcgcgag gaagctgaca aagttactga ttgccccttt gcggtcgcgc
     6301 cagagaaagt agttcagcgt caggctggag gtccatttat gggttttaaa gccctggaaa
     6361 acaatgccga tgatccagcg agatttttgt cgaaccgcag tcgaaaaggt atcggggaaa
     6421 tattcgcgca cgcagatcat gtttgacgtc cgtgcgtgct gtaaaaattt acgctgctcg
     6481 cgttctttgg cttcgtccac caccggaaaa cggacaaaaa tttccgtcat acctttttct
     6541 ttcaggcgga agccaatgtc gtaatcttca gtaagactct gcacgtcgaa agcaataccg
     6601 ttaccgtcag ctaacagtgc ggtcacggcg cggcggctga aacaggtgcc gacgcctgcg
     6661 ctgggcactt gtccggcgag ggcttcacgc accggaacat ctttgccatg cagttctgaa
     6721 aactcatcaa tgtaagtcat gctg
//
LOCUS       DAJTNM010000168         6646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTNM010000168 DAJTNM010000000
VERSION     DAJTNM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.168"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..346
                     /locus_tag="OO172_003764"
     CDS             <1..346
                     /locus_tag="OO172_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5513764.1"
                     /translation="ITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGI
                     SKRGDKNLRRLLVQCARSFMIQLERQHGKLAEWVREQLNKKHSNVVACALANKLARIA
                     WAITTQQKEYQA"
     gene            complement(649..1419)
                     /locus_tag="OO172_003765"
     CDS             complement(649..1419)
                     /locus_tag="OO172_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513765.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1464..1898)
                     /locus_tag="OO172_003766"
                     /pseudo
     CDS             complement(1464..1898)
                     /locus_tag="OO172_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1910..2131)
                     /locus_tag="OO172_003767"
     CDS             complement(1910..2131)
                     /locus_tag="OO172_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513766.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2132..2815)
                     /locus_tag="OO172_003768"
     CDS             complement(2132..2815)
                     /locus_tag="OO172_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5513767.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2879..3190)
                     /locus_tag="OO172_003769"
     CDS             complement(2879..3190)
                     /locus_tag="OO172_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513768.1"
                     /translation="MKYDGDGRATARFFSDKGCRRVPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3187..4089)
                     /locus_tag="OO172_003770"
     CDS             complement(3187..4089)
                     /locus_tag="OO172_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5513769.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4529..5488
                     /locus_tag="OO172_003771"
     CDS             4529..5488
                     /locus_tag="OO172_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5513770.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5488..5883
                     /locus_tag="OO172_003772"
     CDS             5488..5883
                     /locus_tag="OO172_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5513771.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6066..6446
                     /locus_tag="OO172_003773"
     CDS             6066..6446
                     /locus_tag="OO172_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5513772.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6492..>6646
                     /locus_tag="OO172_003774"
     CDS             6492..>6646
                     /locus_tag="OO172_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5513773.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWR"
BASE COUNT         1802 a         1729 c         1618 g         1497 t
ORIGIN      
        1 gattactgcc agcttactat cgtcgcaact tggtgacggg aaacagtttt catgcagcag
       61 agattttgcg gcttccacag ggctcgttcc ccggcagtat agcactggag gaaaatcgac
      121 tctgttgggt atcagtaagc gaggtgataa aaatttacgc cggttactgg ttcagtgtgc
      181 ccgctcattc atgatacagc ttgaacgcca gcatggaaaa cttgctgagt gggttcggga
      241 gcaactcaac aagaagcatt cgaatgtggt tgcctgcgct ctggcaaata aactggcccg
      301 aattgcctgg gcaatcacta ctcagcaaaa agaatatcag gcctgatata tagatgctga
      361 caaaactcaa ttagcttatt gcaaacaata agatacaagt taccgtctgg ttttgcgaag
      421 attgattatt gatgacatga acggcaaatc gacctgatta aaaacctaac aggggaaaat
      481 ggctcactga agccgagggg atttttagga ttatcaggtg cggaactcat cgaggcgcgg
      541 gcatcccttg cccacagaga cgccggatag atttaagcaa gcccaccatt aatcaaaaat
      601 cagtgttgca aaaacggggg tgaccataga ttttccccgc catcgtcatc atttccggat
      661 ttttgccagg gcatccgcca tgctgcggat ttcggcccgg gcttcccgga caacttcccg
      721 cagaacacca cggtcaaacc accggcgggg cggttcatcc ggtatacgca gcacatcgca
      781 ggccagccca ctggacagga tatgctcaat acaatcgctg accagataca tttccaccgc
      841 cgaaaaacgt gcgccccggt tcaggatggc atcgagcacc acgggcaggt tttcatcgtc
      901 ttcatcgagc cagcaggggt gatcgctttc cttcacgacc aggcgaaagc ggcgcgtctg
      961 gcgggtcagc aacgtctgac cgcttgtggt cagcgccgcc cggatacgcc gcgcatcttc
     1021 tgccccccgg caactgacac tgcatactgc atcgcgcacc gcatcaagaa ctgccctgat
     1081 ttcgtcttcg gtcagccatt cacaggcata cgggaaagac acctgcgttc cgtccgccgg
     1141 aagtagcggc agcgactgcc actgactgcc ggacagaaca agttccgctc cggttccggt
     1201 cagtttctga atggcggagg ccaggtgcgg cggcagcacg ccatcgtggc ggttaacgag
     1261 taacgtcgcc agccggggcg catccagccg cacgtaaatg ctgatccagc ccggatagtt
     1321 cgggttacat tggtcaacat ccgtctcttc cggccagccc tgcgccagca gcaggttacg
     1381 ggcgtgacga caaatcagag cattcagcgt ttcattcata attcatgaac tccctgtatc
     1441 gccgcccgca ggcggcgata acatcagtcg ttttcgtgtg tgttgttttt cagcaggctg
     1501 cgggcctgat caaccatcgc ctgtcggcgg gtaacgtatt ccgggagggg gaaaccgtga
     1561 tcccgggcag cccattcacg tttccacagt gcctgttcag cgatctgagt cagggtctcc
     1621 agcagttcag cagggacggt cacctggcgg ttttcgcgca cgttgctgat aatctccatc
     1681 gccgcggcgg aagaaatacc ggactcaatc cgctagtctt ccgggatgcc ctccaggtgc
     1741 gccaggacgg tcctgatttc atgatcggac agggaaatat ccattccgtc agcgagggcc
     1801 tgaatctctt ccggggacca gataaccagc atcagcggcg tgtctcccga atactttgct
     1861 gccagcgccc ggcatagcgt ttcgcatgtt ccgtacatac tctctcccct caatctttga
     1921 agcaggaatc cagttccaga atttcggtat atgcctccat caagaggcgg tttgcgcgaa
     1981 acaacgtctg acgcaccaga cgttccggca gggtgtcagc attaaagctg taccgccgct
     2041 ccatctcatt catggttgta cgcaggtcgc acatggcatt ggccacctcc gccacttcgg
     2101 cgcgaagttt ttcgtgtccg gcatagttca tttacgcagc ctccggcata aaccagtcat
     2161 cattcgcggc cccctgctgc atggcccgct gtacggcagc cagtcgttgc tgtccggcac
     2221 ggtgatactg ctcaagcagc tcgataccga tataccggcg tccggactgg agggcggcga
     2281 cgcaggttga accgctgcct gcaaacgggt ccagcactat cgcattcggg tgtgtgaagc
     2341 tctcaatcag cggttgcagg ctggtaacgg gcttttccgt cgggtgatgg cggttgcccg
     2401 aatatttcca gcccaacacg tccggcagcg ggttttgtgg cagacgtgga cggccttttg
     2461 ccaggatgta ggcgcattcg tggcgatagc ccacatatgc ggccttcgat gtgtagtttt
     2521 tggtgaagac caggtgacca acaacgctga atcccgcatt tttccaggca gacataaagc
     2581 gatcgacgcg gttccagcca tagaagctca ccatcagcgc gtcttttttg agtacgcggt
     2641 acatttcatt acaggccggt tgcagccatt catcagtttt atcaccagca atggtacgcc
     2701 cctgacggtc acgaaaaccg acgagatatg gcggatcggt gaggatgaaa tcaatggcgt
     2761 tatcaggaat acgggccata acatcgatgc agttaccgag gacaaaacgg gacataacag
     2821 atccttctct tacagagggc acccctgtgg tgcccgttcc ccgctgcctg tgggctgttc
     2881 atcgcgtccg gtaacgggtc aggccgaccg tggaataccg cagccgtcgt ctttttgaca
     2941 gcaaggttat gccgcgaaag cggcttgaag ggcgttcatc agaaaagggc gacgggctgg
     3001 cgtacactgt caccgacgcc agccgggagc ggtaaaagcg cccgtcccgt gcccgccgga
     3061 cgcgggacac tgaccacagg cagcgtttat gccgcgccgc gtcagccggc gcagtaaaaa
     3121 gaggaacacg gcgacatcct ttgtcggaaa agaaacgggc ggttgcccgc ccgtctccgt
     3181 catacttcat ccgccataat gcgccacatt gtcgactatc cacgcaggtc cggtgacttc
     3241 cggctgctca tcgtcatctg tcggggaaga ccattcaagt tccccccaca gcacatcgac
     3301 gagctctcca cgctcataca actgccagcc acagaaatca cagccctgtt cggcatacca
     3361 gtgctccagc gtgcagctga aacggcggct taattctgca ataacgtcgc tttccggctg
     3421 acaccacggc gtgtcgaaat ccacctgaat gaagttttct ccctgactgc tgatattcag
     3481 gtcatatcca caaggccact tcacgccata ccgttccgta taccagtgat atgcagaagg
     3541 aacaccgttc agaacaccac cattaaagcc gttcacttca acatccaggc gggtcggcag
     3601 aaccgtcagc aggtcaaacg gcatggtttt ttccggcact acgttgtcac acagacggtt
     3661 ccaccacaca gatacatcct cgttacccca tatgtcacac cagtcacctc tttttgcggt
     3721 gaaatgcaca gtgatggttt ccctggcatc atccggtaat ccttcccagc gacgctggcc
     3781 agtaccgctc tgtaaccaga gttcatgcag catcaggcag ttttgttcat ccagcaacac
     3841 accgttctgc aggtgtgtca gccagcgggt gaacgcgata ttttccgtcg atacagcacc
     3901 acgtccggca gccgtcactc cggggcactg ttcagagcgg atattttcag tgatctgcag
     3961 aagtccggca cttccggcca gaaacagctg aataccttca tttgtggcgc ggcgataaag
     4021 cggtgtgact gcaccgctgg ccagtcgttt aatctcagcg atctgtgccg gttcaccagg
     4081 aaaatacata cgattcgagc accagtttgg catgggaatt ctcctctcac atgcggcctc
     4141 catcaggccg tgccgcacct gtaaccgggc gaccgcatac gaatgcaggt gcggccccga
     4201 agccgcagcg cgtggaggag gaatgacgaa acgtgaccgg gtgaggaggc cggcgacagc
     4261 gacacacttg catcggatgc agcccggtta acgtgccggc acggcctggt taagtagtat
     4321 ccgtctcagt tcgtggcgca aaatgagaaa ccaataagaa ctcaaaatga accacaacaa
     4381 gaacataaag agactctaaa agagcaacac cgcttgacat agactcaaaa tgagtcacaa
     4441 taagaaacaa taaaaaactc aaaaagatgc accgatataa aggatgctca atttgagata
     4501 aacacaaact caaaacgagg caaaaagaat gctaaaagta tcatgcgatg atggttcaac
     4561 taacgtaaaa ctggcctggc ttgaagatgg cgaggtaaga accagtctgt caggaaacag
     4621 ctttaaagaa ggctggaacc cgggactgtt taatgccggc aaagtttaca attacgtcgt
     4681 ggatgaaaaa aaatacacct atgatttggg aagcactgct gtcataggta caacacatgt
     4741 cagttatcaa tacagcacca ccaatctgct tgccatacat catgcactgc tgacatccgg
     4801 ccttcagcca caggatgtag aactgacagt aacattacct gtgaccgaat tctttgataa
     4861 tgacaatcaa ccaaatgaag aaagaataga aagaaaaaag gcaaatgttc tccgtgagat
     4921 atctctgaat aaaggggaaa catttaaaat taaaaaagtt aatgtaatgc ctgaatcttt
     4981 gccagctgca tttgaatcac taaaaaaaga taaagtaaac aaacttgaac gcagcctgat
     5041 tatcgatctt ggaggaacca ctctggattg tggtttaatt ctcggtgcat ttgaaggcat
     5101 ttctgaaatc cgtggctatt cagaaatcgg gacatcccgc atcactcata ccgtcatgaa
     5161 tgcgctgacg aaagcctcca caccatgtaa ttattttatt gctgatgagt taataaaaaa
     5221 cagacacgat aatgaatatc tccagacatt aataaatgat gtagctgaaa taaaaaacat
     5281 ttctcatgta atagacagag aagtgaaatc tctggcagaa agcatcaggc aggaaatttc
     5341 aacattctca ggaatgaacc gaatctatct cactggcgga ggagccgagc ttatttaccc
     5401 tcacataaag caatatttcc cgaatctgaa agtgaacaag gtggatgaac ctcagtttgc
     5461 attggtgaaa gcaatggtgc atgcgtaatg gaaagcagtg atccaaaaaa aagaaagaaa
     5521 gttgtggcat acctgcaccc ggcactttat ccgcaggaca acctgacaca acaaacaata
     5581 gactctctcc cagtacaaat gcggggagat ttctacagac aatcccttat ttgcggagca
     5641 gctctttatt ctgttgaccc ccgactgtta acgctaatca gcggtttttt cagtgagaaa
     5701 atcactgcag aaaatctggt gaaacttata gaacaaacaa caggatacac atccacatca
     5761 attgatataa gtgtgttaaa aaacatcata gaggcatctt cagaaaacaa atcagagagc
     5821 atcacttcaa aagatgattt cgaagaacaa accagacgca atctttctat gttaaaaaaa
     5881 taataaaaga agagcaggtt cagtcaatta cctgctcttc ttttatacat actaaaaaat
     5941 cagattttta cgaatattca gattatcata aaaaatgcaa cagacaggaa tgcaataatt
     6001 ttgcattatc tcgttgcttg taatgcaaat atgcattatt gtcagtgtgt gacaataaca
     6061 gagagatgac gatggcgacc attaacgcac gtattgatga tgatataaat aaccaagcag
     6121 atgaagtgct gaagctgatg aatatcagtc aaactcaggc tattgcagct ttttatcagt
     6181 atattactga acaaaagaaa ctgccatttg tgatcacctc aattgtaaaa acgccgcatg
     6241 atttattacg tgaaagtaca gacatgttag ctgaagctct tgcagtgatt tccaatctac
     6301 aggtgtggac tgaacagcag gatgggattg gaaaagcaaa gctaatggag tattaccgcc
     6361 gcctcgacgc actgtactgt tgtgcaaaag agaaaatagg tcttctttca gataaccggg
     6421 atgctgaact aggctgtgtc ccttaatcat ctgagttata gtaactgtct gtttacacaa
     6481 catattgaac tatggctcgc tacgaccttc ccgatgaggc atggaccatc atcaagcctc
     6541 tgttacctcc tgaacccgcc acaccacggg ccggacgccc atgggctgag caccgtaaaa
     6601 tcatcaatgg catgttctgg gtgttatgtt ccggtgcacc atggcg
//
LOCUS       DAJTNM010000169         6488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTNM010000169 DAJTNM010000000
VERSION     DAJTNM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.169"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..365)
                     /locus_tag="OO172_003775"
     CDS             complement(<1..365)
                     /locus_tag="OO172_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5513774.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            889..1863
                     /gene="hemB"
                     /locus_tag="OO172_003776"
     CDS             889..1863
                     /gene="hemB"
                     /locus_tag="OO172_003776"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5513775.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1969..2820)
                     /gene="tauD"
                     /locus_tag="OO172_003777"
                     /pseudo
     CDS             complement(1969..2820)
                     /gene="tauD"
                     /locus_tag="OO172_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2805..3644)
                     /gene="tauC"
                     /locus_tag="OO172_003778"
     CDS             complement(2805..3644)
                     /gene="tauC"
                     /locus_tag="OO172_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5513776.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAVTRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQW
                     VNV"
     gene            complement(3641..4408)
                     /gene="tauB"
                     /locus_tag="OO172_003779"
     CDS             complement(3641..4408)
                     /gene="tauB"
                     /locus_tag="OO172_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5513777.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4421..5347)
                     /gene="tauA"
                     /locus_tag="OO172_003780"
     CDS             complement(4421..5347)
                     /gene="tauA"
                     /locus_tag="OO172_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5513778.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDDKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(5614..6129)
                     /locus_tag="OO172_003781"
     CDS             complement(5614..6129)
                     /locus_tag="OO172_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513779.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            6322..>6488
                     /locus_tag="OO172_003782"
     CDS             6322..>6488
                     /locus_tag="OO172_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5513780.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERV"
BASE COUNT         1600 a         1719 c         1572 g         1597 t
ORIGIN      
        1 gttgtcatgc actcggaata aatcataccg tgaatagtgc tgtttttgat tatcagacta
       61 ttggcgttgg tattggtaga aataccgtcg tccagatagt aggtagaaat cacgccgtta
      121 acggtagcgt tattaatgac cgggtagata tcgccgttgt aatagctatc agcggcatag
      181 ttgttccagc ctacataacc atcatagtaa acgtcatcag cataggtggc gtcgttgtag
      241 tgatggaaag tattgtaagt ttgaccagaa atatcgttgg tcgctgcatt agcctgagag
      301 gtgatagcca gagtgcaagc caatgctaat tgtgatacta caagtttctt tttccaggag
      361 tgcatttgtc atccccctca gggacgtaat aagttgatcc atcaattatc aatgcataga
      421 aaacatatta tgtttcgcgc taacgattat gctgcgttac agacgaatgg ttcaattatt
      481 ctttccccta agtccgaatg tggacatata taatttatgg agaaaaacac gcatcaaatt
      541 gataaaagga cgatatttca gcatgttgca gataatgtta tttttgatgc atacgcgtta
      601 taaatatcaa aaacacaaac cagataaata tcttatcaat atacaacaaa gtttgaatta
      661 ttcaatttat agcagcacaa ccaaacgcca aaaaaataat aaccaagagc tataaataaa
      721 gattaaaaat attttcagtg aatatgaaat tattgctatt tctattacaa agatgtcctg
      781 gtctctcttt cattctcttc ttgtcaacat cgcgacaact ttcgtaaaac actcctgccc
      841 cgcttcaggt atactgtgca ccttaattcc acaaacatca ggcagaccat gacagactta
      901 atccaacgcc ctcgtcgcct gcgcaaatct cctgcgctgc gcgctatgtt tgaagagaca
      961 acacttagcc ttaacgacct ggtgttgccg atctttgttg aagaagaaat tgacgactac
     1021 aaagccgttg aagccatgcc aggcgtgatg cgcattccag agaaacatct ggcacgcgaa
     1081 attgaacgca tcgccaacgc cggtatccgt tccgtaatga cttttggcat ctctcaccat
     1141 accgatgaaa ccggcagcga cgcctggcgg gaagatggac tggtggcgcg tatgtcgcgc
     1201 atctgcaagc agactgtgcc agaaatgatc gtcatgtcag acacttgttt ctgtgaatac
     1261 acttctcacg gtcactgcgg tgtgctgtgc gagcatggcg tcgacaacga cgcgactctg
     1321 gaaaatttag gcaagcaagc cgtggttgca gctgctgcag gcgcagactt catcgcccct
     1381 tccgccgcga tggacggcca ggtacaggcg attcgtcagg cgctggacgc tgcaggcttt
     1441 aaagatacgg cgattatgtc gtattcgacc aagttcgcct cttcctttta tggcccgttc
     1501 cgtgaagctg ccggaagcgc attaaaaggc gaccgcaaaa gctatcagat gaacccaatg
     1561 aaccgtcgcg aggcgattcg tgagtcactg ctggatgaag cccagggcgc agactgcctg
     1621 atggttaaac ctgctggagc gtacctcgac atcgtgcgtg agctgcgtga acgtactgaa
     1681 ttgccgattg gcgcgtatca ggtgagcggt gagtacgcga tgattaagtt cgccgcgctg
     1741 gcgggtgcta tagatgaaga gaaagtcgtg ctcgaaagct taggttcaat taagcgtgcg
     1801 ggtgcggatc tgattttcag ctactttgcg ctggatttgg ctgagaagaa gattctgcgt
     1861 taattttttt cctctcagaa agggggagtt aacctgttga aagtacggtg aacgccccct
     1921 caccctaacc ctctccccaa gggggcgagg gaaccgtcca cactcgtatc accccgcccg
     1981 ataaaacggt ttatccccaa ggatcgtcgc ccgatgcata atccgccgct gtggcaggta
     2041 atcggcattg gcatagtgct gagtcacgcg gttatcccaa atcgcaatat cattcggttg
     2101 ccagcgccaa cgcacctgaa actccggttt agtgatatgg gcgaataaaa agcctaacaa
     2161 ggcttcgctc tctttctcgc tgacatcgac aattcgcgta gtaaagcctt cattcacaaa
     2221 cagcgcctgt ttaccgctca ccggatgcgt tcgcaccacc ggatgtagca acggaggatt
     2281 tttcgcgacc gcctcgcacc agcgttgatg ttcctcctcg gttttgcggt atttgtattc
     2341 cgggaatgat ttacggaaat catgctccgc acgcagccag ctcagcagct ggcggaaggg
     2401 aacagagagc gcctcatagg ccgcaatacc gctggttcag agcatatcac cgccggtcga
     2461 aggtaactct ttagctgcca gaatcgcccc tgcgggtggc gtttcaataa atgtcacatc
     2521 ggtatgccag ttgtcgttat ccggcggatt atcgttatgg gtatccagca cgatgatctc
     2581 gtcaacccct tcggcatgag ggtaaacagg gtgaatatgc agttcgccaa aacgctgggc
     2641 cagcgcgcgt tgctgctgcg gcgtaatagc ttgatcgcgc agaaatacca cctgatggcg
     2701 cagcaccgca tggtaaagct gttcaaactg attatcgctt aacgggcgcg tcaggtcggc
     2761 acccgaaatt tgtgcgccga tatgcggccc cagcggggta atgctcagac gttcacccat
     2821 tgtacttctc catgccaggg cgtcagacgg cgctgtaacg cgcgcagacc cagttctaaa
     2881 agaaaggcga taatcgcaat caccgcgatc cccgccagta ccacgtcagt cgcgagaaat
     2941 tcacccgctg actgaaccat aaatcctaaa ccgcgtgtca cggcaatcag ctccgccgct
     3001 accagcgtag accagcccac acccagacca atgcgtaatc cggtgaggat ttccggcagc
     3061 gcgccgggca aaatgacaaa ccacagcacc tgcgcacggc tggcacccag cgactgggcg
     3121 gcacgaatgc gaacctgctg cgcgcttttc acccctgcca gcgccgacat cgccaccggc
     3181 gcaaaaatcg ctaaatagat cagtaagatc ttcgaggttt caccaatacc aaaccagatc
     3241 accatcagcg gcaaataagc cagcggtggc accggacgat aaagttcgat taccggatcc
     3301 agaatgccgc gcaccgtcgg gcttagcccc atggcgatcc cgaccggaat accgatcacc
     3361 accgccgcga gtagcgcctg cacaatgcgc gtcaggctgg cagccagatg ctgccacagc
     3421 gtggcgtcca taaatccctg cggtccggca atggtgagca gttttgccag tacttgttgc
     3481 ggcggcggca aaaacagcgg gctaatcagt tgcagcgccg ccaccgtcca ccatacagtg
     3541 agtaaaaccg ctaacgtgcc aatgcttaag gtcacctgac gcgagagcgg ccagcgccat
     3601 ttcagccgcc gcgaatgcag tttttcatta atgagcacac tcatgagaac gcctcccgtt
     3661 gctcaaatac gcggcttaaa acatattcgc gcatggcgat gaattgtgga tcggacttga
     3721 tgctgcggct cgactctccc gcaacaaagc ggcgagcaaa gttgagcggc agccgctcca
     3781 gcacacggcc agggccggat gaaagcagaa ccagttcagt cgccataaac accgcttctt
     3841 ctatatcgtg ggtaatcaac agcacctgct tgcccgtctc ctgccagagt ttcagcagca
     3901 gagtttgcat ctggtcgcgg gtgaaggcgt ccagcgcacc aaacggttca tcgagtaata
     3961 acagctgggg attcgccgcc agcgcgcggg caatccccac ccgctgacgt tgaccatcgg
     4021 aaagctgcca gatatagcgt ttttctgcgc cttccagccc cacttttttc agcatctggt
     4081 gcgcgatttc cagtcgctgc attttctcta tacctgccaa ttgcaggccg aacgccacgt
     4141 tgtcctgtac attgcgccac ggtagcagcc cttcattctg aaaaactacg ccacgctctg
     4201 ctcccggtcc ctcaatacgc ttacccgcca gttgaatgct gccatgctga taaggcacaa
     4261 aaccggcaat cagattcagc agggtggttt taccgcagcc ggacggcccc agcaccacca
     4321 gtagctcgcc gctttccagc gtcaggttga tatcttccag tgccggtttg ccgccataat
     4381 cggcatagag atgagagatt tgcagcatct gcgcctcctt ttattgcacg aagcgcgagg
     4441 taacgtactg gctgtaatca ttcgctacag ccgggacctt gccctgctct ttcaaaaact
     4501 gcgcggtgtc gatgattgct ttgttcaccg gtccggtcag ttctgccgtt tgttgctgcg
     4561 gcgtcagata ggtatttccc ttcaccagcc ccggaacgtc accttcaggc acgccgctta
     4621 aacgcgccag tttgctgatg ttttccggct gtttcagcca cgcgtcaggg ttagcaatgt
     4681 acggttgctg tgcatcgatg gcgcttttgg cgaacgcttt cacgacctca ggatgtttct
     4741 cggcaaaatc tttgcgcacc acccagacgt ccagcgttgg cgcaccccac tgcccgacct
     4801 gttcagaatc ggtcagcacc ttgtcgtctt tttccagggc gttaaccgcc ggtgcccaga
     4861 cataagcacc atcaatatca ccccgctgcc atgcggcgat aatcgcgggc ggctgcaggt
     4921 tcacaatctc cactagccct ggtttaatcc cccagtgttt cagcgccgcc agcaggctgt
     4981 agtgggtggt ggagataaac ggtacggcaa tgcgtttacc aatcaaatct tcgggtttgc
     5041 tgatagtttt ctttaccacc agcgcttcgg agttacccag ttttgacgcc agcaagaaga
     5101 cttcaatcgg cacctgttgg ctggctgcaa ccgctaatgg gctggatccg aggttgccga
     5161 tttgcacgtc gccagaagcc agcgcccgca cgatgctggc tccgctgtca aacttacgcc
     5221 agtccacgct cgccccgctc tctttagcaa aggtgttgtc ggcctgagcc actttcgccg
     5281 gttcggctga ggtttgatac gccacggtga cgttcaccgc ctgtgcctga aaagcgatga
     5341 atgccagtgc ggcaagaagt gtgttatatg actaggtaat ttattcaaga taaccgggaa
     5401 tgggtaaaaa aagacgggat ttgatgggat tcaataagtt atgcgccgat tgcattcaag
     5461 ataaaacgag ttaaaaatgc tcaatgcttc gatatcgata aaacattcaa gataggcgcg
     5521 ccgtgtattt taattctttt aagactcttc aaaaatgtca tcgtagcctt taaaaacgaa
     5581 ttttaaaggc accctaaagg caggtcaatg aagttaaccc gccttcgcgt ttacactctc
     5641 tgagagaata ctagcttcaa tctcttccaa aatctctctg acttgctcgt cgggcatatc
     5701 aacgagcatc aacgctgcca ggcgtttacg gccctctagt tgcagtagct cttgagtttc
     5761 cttatctaac aagcgtagca cagtctcaac acctttcctt gcgagcatgt ttgtaagggc
     5821 ttctcgttcc tcactggtaa gaaacgacaa catttctgtt agctgatccc cttggcttaa
     5881 atcactttgt gttttttgtg tttttggtgg attcccgtta ccggttttta gccattcaag
     5941 actaacacct tcactttctg caatgcgtac caatatgctt tctcgaggtt ctggtgtatt
     6001 tcccttttta aaatagcttt gtaacgtatt gatatttatg ccacaggctc tggcggcagc
     6061 acttcttgat ggataccgcc taataagata agccatgcgc tccacgatag gttcttttgc
     6121 ctgctccata aaagtacctc taggtatttt tctgtataaa ggttcttttc catctatcta
     6181 attgaatgga aaggtaattt taaaaatcca aataaaatac aaaaaaatac cttgtattgg
     6241 tgctttttgt gatctgcgat tttatttact cggatatcca cgcgaatatc cgcgcgggta
     6301 attttgtagg gtaaaggaaa gatgagacaa agagaagata gtggccgcga ttggccttcg
     6361 ctgatgattc agcaagcact caaagataat gggtggtctt cctttcggga gctttcactc
     6421 catgccggtt atgctcagga ttcactgaaa gcggtagttc gtacgccaaa caaaaaatac
     6481 gaacgtgt
//
LOCUS       DAJTNM010000170         6434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTNM010000170 DAJTNM010000000
VERSION     DAJTNM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.170"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..433)
                     /locus_tag="OO172_003783"
     CDS             complement(<1..433)
                     /locus_tag="OO172_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR5513781.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGAPIK"
     gene            751..1392
                     /gene="udk"
                     /locus_tag="OO172_003784"
     CDS             751..1392
                     /gene="udk"
                     /locus_tag="OO172_003784"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5513782.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            1484..2065
                     /gene="dcd"
                     /locus_tag="OO172_003785"
     CDS             1484..2065
                     /gene="dcd"
                     /locus_tag="OO172_003785"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5513783.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            2087..3940
                     /gene="asmA"
                     /locus_tag="OO172_003786"
     CDS             2087..3940
                     /gene="asmA"
                     /locus_tag="OO172_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5513784.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4392..5975)
                     /locus_tag="OO172_003787"
     CDS             complement(4392..5975)
                     /locus_tag="OO172_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5513785.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(6176..6325)
                     /locus_tag="OO172_003788"
     CDS             complement(6176..6325)
                     /locus_tag="OO172_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513786.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
BASE COUNT         1630 a         1631 c         1678 g         1495 t
ORIGIN      
        1 acttaatagg ggcacccccc aacagaggcg gaacaatggc gctgccgaat gccagacgca
       61 gccagtcagc cagattttgc aaggggttat accacggcag caatttacgt agcagcactg
      121 ccccgaccac ggcttcaaca atattgatgg tcgtccaggt catgttcagc gagctggtgg
      181 aaaaaagcag gatggatgcg gcgatatttc ccagcgaaca gctcagcgca attcccggcc
      241 acatgcgccc ggcatggcga taaaacgcca ccatcatgat ggacgtcggg aaccacagtg
      301 gggcgagttg ggtgccaaac tgcgaaagct caagcgagac aagagtaaag ataaacgaga
      361 ctaaacctaa actgaccagg tgaagcagcg ggtggggcag ggcaattaat acatgctgtg
      421 attgtttgct tattaccgct ttatccgcaa ggccaggtcg ccagtattcg tttctgtgtt
      481 tatgctagta caaaagtttt acattttata tgcgaattgc tcataaagtg acgtaaaggc
      541 ggataatttg cgcaactgcg tataacattt tttaccttac ataaaactga tcaacgtaat
      601 ttgccaccgg gaattctttg tcaacaatta actctgagcg cgcggcaacg ctattcgact
      661 ggtatcagac ggatgaaatc cctataattg ccgcgtttgg cgcttcgtcg cccccttcct
      721 aacatccagg ttaatcaggt cgctaaatat atgactgatc agtctcatca gtgcgtcatt
      781 atcggtatcg ctggcgcatc ggcttccggc aagagtctta ttgccagtac cctttatcgt
      841 gaattacgtg agcaagtcgg tgatgaacac atcggcgtaa ttcccgaaga ctgctattac
      901 aaagatcaaa gccatctgtc gatggaagaa cgcgttaaga ccaactacga ccatcccagc
      961 gcgatggatc acagcctgtt gcttgagcat ttacaagcgt tgaaacgcgg ctcggcaatt
     1021 gacctgccgg tttacagcta tgttgaacat acgcgtatga aagaaacggt gaaggttgag
     1081 ccgaagaagg tcatcattct cgaaggcatt ttgttgctga cggatgcgcg tttgcgtgac
     1141 gaacttaact tctccatttt cgttgatacc ccgctggata tctgcctgat gcgccgcatc
     1201 aagcgtgacg ttaacgagcg tgggcgttca atggattccg tgatggcgca atatcaaaaa
     1261 accgtgcgcc cgatgttcct gcaattcatt gagccttcta aacaatatgc ggacattatc
     1321 gtgccgcgcg gcgggaaaaa ccgcatcgcg atcgatatat tgaaagcgaa aataagtcag
     1381 ttctttgaat aagcttgata aattgtgtac cgttcagtga taagctggta tgcccttgac
     1441 gtaattggcg ttaagggagt gatgcgcgaa aggagaaaat gccatgcgtc tgtgtgaccg
     1501 agatattgaa gcctggcttg atgaaggccg tttgtcgatc aacccacgtc cgccagtgga
     1561 gcgtattaac ggcgcgacgg tggatgtacg cctgggcaat aaatttcgta ccttccgtgg
     1621 tcacacggca gcgtttatcg atctgagcgg tcccaaagat gaagtgagcg ccgcgcttga
     1681 ccgcgtgatg agcgatgaga tcgttctcga cgagggcgag gcgttctatc ttcacccagg
     1741 agagctggcg ctggcggtga cgctggagtc ggtgacgctg ccagccgatc tggtgggctg
     1801 gctggacggg cgttcctctt tggcgcgtct ggggctgatg gtgcacgtca ccgcgcaccg
     1861 tatcgatccg ggctggtctg gttgcattgt gctggagttc tacaactccg gtaagctgcc
     1921 gctggcgctg cgtccgggca tgttaattgg tgcgctgagc tttgagccgc tttccggccc
     1981 ggcggcgcga ccttacaacc gccgtgaaga tgcgaaatat cgcaaccagc agggcgcggt
     2041 agccagccga atcgataaag actaattcag gcccattgag gataccatga gacgatttct
     2101 gacgacgctg atgatactcc tggtcgtgct ggtggccggg ttatctgcgt tagtgttgct
     2161 ggtgaatccg aatgatttcc gcgactatat ggtcaagcaa gttgctgcac gtagcggtta
     2221 tcaattgcag ctcgacgggc cactgcgttg gcacgtctgg ccgcagctta gtatcctctc
     2281 cgggcgaatg tctctcaccg cccagggcgc aagccagcca ctggttcgcg ccgacaacat
     2341 gcgtctggac gtggcgcttt taccactact gagtcatcaa ctgagcgtta agcaggtgat
     2401 gctaaaaggg gcagtgatcc aactgacgcc gcagacggaa gcggtgcgca gtgaagacgc
     2461 tccggttgca ccgcgcgaca ataccttgcc ggatctgtca gacgatcgcg gatggtcgtt
     2521 tgatatatcc agtcttaagg tggcggacag cgttctggtg ttccagcatg aagatgacga
     2581 gcaggtgaca atccgcaata tccgcctgca aatggaacaa gatccccaac atcgtggctc
     2641 atttgagttc tccgggcggg ttaatcgcga tcagcgcgat ctcacgatat cccttaacgg
     2701 tacggtagat gcttctgatt atccgcatga tttaacagcg gctattgaac aaattaactg
     2761 gcagttgcag ggtgccgatt taccaaaaca aggtattcag ggacagggga gtttccagtc
     2821 ccagtggcag gagtcacata aacgcctttc atttaaccaa attagtttga ccgccaatga
     2881 cagtacgctg agcgggcaag cacaggtcac gctgacagag aaaccggaat ggcagctgag
     2941 actgcaattc ccgcaactga atcttgacaa cctcatcccg cttaatgaaa ccgcgaatgg
     3001 tgaaaacggt gccgcgcagc aggggcagag ccaatcaacg ttgccgcgcc cggtcatttc
     3061 ttcgcgtatt gatgaaccgg cctatcaggg actgcaaggc tttacagctg atattttgtt
     3121 gcaggccagt aacgtgcgct ggcgcggaat gaattttaca gatgttgcca cgcaaatgac
     3181 caacaagtcg ggtttgctgg aaattactca actgcagggc aagcttaacg gtggaaaggt
     3241 ttcactgccg ggcacgctgg acgcgacatc aataaatccg cggataaact tccagccacg
     3301 gctggagaac gttgagattg gtaccattct gaaggcgttt aactatccga tttcgttgac
     3361 cggaaaaatg tcactggctg gtgatttctc cggtgctgac atagatgccg acgcattccg
     3421 ccacaactgg caagggcagg cgcatgttga gatgaccgac acacgtatgg aagggatgaa
     3481 cttccagcag atgattcagc aagcggtaga gcgtaatggc ggtgatgtga aggccgctga
     3541 aaacttcgat aacgtgacgc ggcttgaccg ctttaccacc gatttgacgt tgaaggatgg
     3601 cgtcgtgacg ttaaacgaca tgcaaggtca atcgcctgtg ctggcgctga caggggaagg
     3661 catgttgaat ctggcagatc aaacctgcga cacccagttt gatattcggg tcgtgggtgg
     3721 ctggaacggg gaaagcaaac tgattgattt cctcaaagaa acgccagtac cgctgcgggt
     3781 ttatggcaac tggcagcaac tcaattacag cctgcaagtg gatcagttac tgcgcaaaca
     3841 tctacaggac gaagcgaaac gtcgcctgaa tgactgggcc gagcggaata aagattcccg
     3901 caatggcaaa gatgtgaaga agttgctgga gaagatgtag gttaccaggt gacctgtcct
     3961 gtaggcctga taagacgcgt aaagcgtcgc atcaggcgtt cgcggcggca ctattatatt
     4021 ctgaatatgt aagatcgaag gctgttccgt tcacttgatg ttcctcgcta ctatggaacg
     4081 ctcaagccgg tgaacgtgcc agggtggctc aatcgccacc accctggcaa cccgggctcc
     4141 cggcggtaaa tcgccgctgg cgcggttccc tcatcttatt cctttcggct atcgggtacg
     4201 ggcgcgaggt aacatccctg taaaacgcgc cctcagccca catccatgtg ggctgcccca
     4261 gcctacagga aacgattcgg cgatttacag ccggacccac acccccgctg tgatgtccgt
     4321 ttaccacaga caacgcttca ttgctgaagt tataaggcgt tacgcagcat tgggtaaaat
     4381 ggccgatacc atcacacctc gtactccatc tcgccatcct tacgcagcgg tataatctgc
     4441 accttctgca cgcgatggct ttccacctgc aacgttttaa gcaaataatc ccccacctga
     4501 acttcttcgc caggttttgg aatacgctgc aaatactcca tcaacagccc ggcaatggtg
     4561 tgatattcac gtttttcatc cagcggcagc ggcacatatt gcaccagatc ctccagcggc
     4621 atatgaccat tcgccgtcca ggaaccgtcc gcattcttct gaatatcatg gcgggcgtcg
     4681 atctcttcca cttcgttcgg taagttaccg gcaatggttt cagtgacgtc acttaatgtc
     4741 acaatccctt ccaccgagcc aaactcatcc accacaaaag caaagtgcgt gcgggcatta
     4801 cggaactgct ccagggctgg taacaacggc aaggtttccg ggaacaccag cggctggcga
     4861 atcaacaccc gcaggttgag cggttcgccg cgcagtgact gttgtagcag gtcgataacg
     4921 tgaacaacac cgagcaaatc ttctgcgtca tcaccgtcgg taaccaccag ccgcgtatgc
     4981 tgatttcgct ccagcaactg gcggatttct tcttccggcg cgttgagatc gatatgctca
     5041 atatcgtggc gcgacgtcat aatgctgctg acggtacgct ggttaagatt aagtacccgc
     5101 tcaatcatcc gccgttcctg cggattaaag atctgctggt ttccatgatc caccagcatg
     5161 gacgcggttt cggcgtccag ttccgcatct tctttttgcc cgctaagcag gcgcattacc
     5221 gcttcggtag tccgctggcg tagcgtctga ttagcggaaa gaaaacgtcg ccggttaaag
     5281 atagccaact gattgagtgc ctcgatcatc accgagaaac caatggcagc gtacaggtag
     5341 cctttcggaa tgacgaagcc gaaaccttct gccaccaggc taaagccaat catcaacagg
     5401 aagcttaagc agagaataac gatggtcggg tgactgttaa caaattgcgt taacggcttg
     5461 ctggccatca acatcaggct gatagcgata accacggcgg ccatcatgac cagtaaatgg
     5521 tcgaccatcc cgacggcggt aatcactgag tcgagtgaga aaatggcgtc cagtaccaca
     5581 atttgcgtca ccacgcccca gaacttcgca cctttgcgtt gtgtgggatt attgctgtct
     5641 ttcccttcca gccgttcgtt cagctccatc gtggctttga acaacaggaa gaaaccacca
     5701 aacagcatga ttaagtcgcg ggcgctaaag gtaaacgagc ggaagctgaa cagcggttga
     5761 gtcagggtga ccagccagga gattgacgct agcagtaaca ggcgcattaa catcgccagc
     5821 agcagcccgg taacccgtgc gcggtcgcgc tgcttcggcg gtagtttttc ggcgaggatg
     5881 gcaataaaga ccaggttatc aatgccgagg accagttcga tcacaatcag cgtgattaac
     5941 ccggcccaga tagacggatc ggcaatccat tccatagtaa gtacaaaacc tattcgttgt
     6001 atgacaaatg tcacaattcg atcatggata aatcggggca aaaatgcaat gttgcagcac
     6061 tatttatctc atattatttg agggtaataa cctcacatta tccctgaatt aaaagcggta
     6121 ataataaaac attatttaag aaatatcgca tgtggagacg caatattaaa tattgtcaac
     6181 ctaaagaaac tcctaaaaac catattgaat gacacttaat ataattctta aaaatagcca
     6241 attgccgaat tgttaccttg cctgctattc cgttagctgt aacacttcct cctgcattat
     6301 tggcgagcca atattcaatt accataatgt caccttatat tcggaagtta atttccgcaa
     6361 taaccctttc tcatcaacga ttgtatgcag agagggtttg aaaaattaag ctcaatcagt
     6421 gtattggtag ctaa
//
LOCUS       DAJTNM010000171         6415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTNM010000171 DAJTNM010000000
VERSION     DAJTNM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.171"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(206..367)
                     /locus_tag="OO172_003789"
     CDS             complement(206..367)
                     /locus_tag="OO172_003789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513787.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(364..552)
                     /locus_tag="OO172_003790"
     CDS             complement(364..552)
                     /locus_tag="OO172_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5513788.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            812..1147
                     /gene="iraM"
                     /locus_tag="OO172_003791"
     CDS             812..1147
                     /gene="iraM"
                     /locus_tag="OO172_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5513789.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1428..1559)
                     /locus_tag="OO172_003792"
     CDS             complement(1428..1559)
                     /locus_tag="OO172_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR5513790.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2059..2135)
                     /locus_tag="OO172_003793"
     tRNA            complement(2059..2135)
                     /locus_tag="OO172_003793"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2099..2101),aa:Arg,seq:tct)
     gene            complement(2237..2312)
                     /locus_tag="OO172_003794"
     tRNA            complement(2237..2312)
                     /locus_tag="OO172_003794"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2277..2279),aa:Ile,seq:cat)
     gene            complement(2456..3277)
                     /locus_tag="OO172_003795"
     CDS             complement(2456..3277)
                     /locus_tag="OO172_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5513791.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3292..3648)
                     /locus_tag="OO172_003796"
     CDS             complement(3292..3648)
                     /locus_tag="OO172_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5513792.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3661..4710)
                     /locus_tag="OO172_003797"
     CDS             complement(3661..4710)
                     /locus_tag="OO172_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5513793.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRSWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(4712..4990)
                     /locus_tag="OO172_003798"
     CDS             complement(4712..4990)
                     /locus_tag="OO172_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513794.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            complement(5057..5307)
                     /locus_tag="OO172_003799"
                     /pseudo
     CDS             complement(5057..5307)
                     /locus_tag="OO172_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5525..5680)
                     /gene="hokD"
                     /locus_tag="OO172_003800"
     CDS             complement(5525..5680)
                     /gene="hokD"
                     /locus_tag="OO172_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5513795.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(5752..6039)
                     /gene="relE"
                     /locus_tag="OO172_003801"
     CDS             complement(5752..6039)
                     /gene="relE"
                     /locus_tag="OO172_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5513796.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            complement(6039..6278)
                     /gene="relB"
                     /locus_tag="OO172_003802"
     CDS             complement(6039..6278)
                     /gene="relB"
                     /locus_tag="OO172_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5513797.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
BASE COUNT         1704 a         1713 c         1351 g         1647 t
ORIGIN      
        1 cccaccgctg acgtggtgta tgacacacct gttgtgattt tttccatctg gtccataccc
       61 cgtctcccgt tatccggaag ctgacaacaa taaaaaagcc accagttaac tactgatggc
      121 tctgataact catgcaagcg tctcagacga cccactgaca ctaccggtga gtttaacgat
      181 accttccatt tgactggctc actttttatg atgatgccgg tgcatttatc tccagcacca
      241 gactttctat ctcaacgcca tacgttgcat ttttggtaat atccgtcagc gtcagtgcat
      301 ttagtcccac tgccagactg tcttttatgg cctggaatgc cgggccagta cgatgacgta
      361 gtatcactcc ggctcagttg caccactgac caccacatca ccttctgctg caatcgcctg
      421 catcagggta taaggggtta tggccaccgg actaccaaac ggctgccagc cctctttcag
      481 tttatgtgtc agcttttccg caagatctga cggcggcgcc gccctgacaa catcatagtg
      541 tttaaatgcc atggttcttt ccactatctg aaaaataatt ctttaaaata cctgacatgt
      601 aatacagaaa aaacacaaaa ccatacctta aataaaaacc tgattatcaa gcagatatgc
      661 atggataaac tacaagacga gatataaacc accctgcatt taaataaaca ataaacaaca
      721 tcagaaaaat aattctgctc tatggtttaa ttcaaaaata tcatttatac ttttcagaac
      781 atcaccagca aggcataaac aaggaaacta aatgaagtgg attgtgattg atacagttat
      841 ccagccatca tgcggaatat ctttttcagt catatggagt aaaataaaat taataatctg
      901 gtatcaatcg gatgctttct tacctcctga aagtatattt acactgactc acacaggcat
      961 catgctcaat aacaaagtgc tacctgtaac catttacaac gtagtaccat tcaataaaac
     1021 attctggaat ttaatcaaaa acagccagga atgccctaca aatacagata acgtattgaa
     1081 tgaatgcttt aataaccgtt gcactctgca aatatgtcct tatggactaa aacaacaaag
     1141 tccataagga gtttactcac atctgacaaa atcaatataa acagcccctc cggagagggg
     1201 ctggagagtg gcgctatgtg ccattgcatg gtgccgggtg cctcccggtg aattcagtac
     1261 cagcacctga atccgcgatt atcccatata cctactcgct gattgcccct ccgcacaggg
     1321 ggattcacca tgccagtttc ttttaacaaa ctccccgcaa accagacaac agtcaaccgc
     1381 ctgaattgtg aagtatttaa aaatttctcc cgctaactga tacccggcta acagtctggc
     1441 gttttctttt tcagcaacgg gaaagcaaca accaccacac ccgccaccag cacaccgtca
     1501 gccggcactg acattatccg gctgctgcaa tgccattcac aaaaacagta agcaatcact
     1561 ttttaccgta acaggtgata atccagatat gtatctaccc cagatgagta atccgaagtt
     1621 catccatacc acaggtcctg gctattctgt tgtactcctg aacaagagca aataattctg
     1681 aattagcaac catgaactca tcgcaaaccc tctgtatagc atcactattc agaataataa
     1741 cgtctcttcc cgaaagacga tcaggagtac agaacaaaac tgtcaaacgg ctgaaggcct
     1801 ttgctcgtgc tgcattgact atatcaatac gctgcctaag gatgaaacac cccgacgcct
     1861 catcaatatt cactctaccc acaccatatg aatgataaat acttaatgct gaaaaaacca
     1921 ttagaccgta taacaaatac tcaatcaata cttaacagaa cttttatttt tgacaaatat
     1981 ataatatttt caacaatatc ctgagccagg tatatttcag tataaggctc tgccggaagg
     2041 aatctggaag aatgaatatg gcgcgctgta ctggattcga accagtgacc gattgcttag
     2101 aaggcaattg ctctgtccgg ctgagctaac aacgctgaat accgataatg gaccgccatc
     2161 ggggacccgc cccccgcacc aacaaccctg ttatcgtgtc gtctgctctt cctgataagc
     2221 taatggcggt ttgtgatggt ggcccttgct ggatttgaac cagcgacctg gcgattatga
     2281 gtcgctcgct ctcaccactg agctaaaggg ccgggagcag aataataacg gtccgtaatt
     2341 aatttcgcaa taaaaaaccc gctcagcggc gggttgtaga aactcttcta acgtcaggca
     2401 taaaacgccc atcattatga cgaatttacc acagattccg gaaaaatcaa ccttgttacc
     2461 tagttacctt ttttaactgc cgctcagccc atgcttcttc aatatcaaac cgggtcacca
     2521 gcgcatcata aaatttctta actgtttttt cccatgacgc gcgtgttatc tggtttgtca
     2581 cctcgcatat agcattaaat gcctccgttg atggtagtct ttcatagcca cgaccaccac
     2641 aacgctggca gtctctgata acaggcatac cacgttttac cgactcttca cgatgaatgg
     2701 cgacaccacg cccacggcaa tccttacagg cggtggaaac ctcacccttt ccgccacact
     2761 ccggacaggc aacttttacc acctccctga cttttttcca ttcttcccag taagacggat
     2821 acacaccttt cgtacacttt gcccataccg gcggcttacc atccggatac tggatcttgt
     2881 ttgtaaaaac tatgctttca ataaattttt ccccatagca acaagggcac tgctttttac
     2941 tcgctgcgct gcgggcataa tcctcaaaag cgtacgaagc cataatgcgc atcactaccg
     3001 gttttatttc tgccggaagt tttctcaacg ccgccacacg atcgcaccga ctgagtgcat
     3061 aatctgccag taattctgtt gcccgcgccc tgtcattcat actgatgccc attttcccca
     3121 ggaacgcaga aaaacccatc tcagcccgat tctgtgtcat gccctgcgcg gccatcacat
     3181 cagtgatact cagcgcatct tttgacgttg aggccgatgc atcggtcagg ccaggggatt
     3241 ttggggagta gtatttcggt aaatcttcca gtttcatttt ttgacctgct cttaatgcat
     3301 tatggggtaa atcttcaccc ccagacgtcc accagatact ggctgaccac gaacgatatt
     3361 gatttcatca aactgctcat cgtccattaa cactcccgca tgcgtcagcg catccagcgg
     3421 tgctttcagg atattgtcca ggtcgcgacg acgcttatcc ggtggctctg caatcacctt
     3481 tatcgccagc cttccggaca ggcttaattt cagccgctgc tggcgaacaa taagcgccac
     3541 agcccggcga taacgctttc cctcctccga gataaaatat gtgctgccac ggcgtcgcca
     3601 gtaagtgttc accgtcggcg ggtaaggtaa aaccaaatct atgagcatca gtcacctctt
     3661 ttacccaagc acgccagttg caaaggcgtg atcaagaaaa cgaaaaatta aatcaacctg
     3721 agaaccatgc ttttcttcga acgccagagg atccgcatga agctcgttgt gatgctcccg
     3781 acacagcggt agcgtgaaaa tatcgtgaga ttttgtcccc attccgccct gaccatgacc
     3841 aatcaggtga tggggatcgt cggctggctt accacaacac gcacacggct gtgtcttcac
     3901 ccagcgtttg tatttctcgt taacccagct gcgacgttta ggtcgtttca tgaaagattc
     3961 cggagactca ggatcaacgg caatgctgac caccgtcttt tcctgttgtg ggttctgttg
     4021 ctggtgggcg tgaggcagcg gcgcaagatt ttttgtgcgc tgtttcagta tgctggtggc
     4081 ggtctgctct cccggtacga tgtcgctttc acggtacatt gagcggattt tttccgcacg
     4141 caaccccagc gaacgacgta ataccgcttc cggtagcgcg tccgccacct gattgcggac
     4201 cgcccaccag gataattcag ccagagataa ttcacgctcc tgcgtaccgc ttattgcgtg
     4261 accgatgacg tcaatcatcc atgctgacag gttttgatga gcaagttgct cgagtgattc
     4321 ggatgtctgg tcacgcagct ggttgtcgca gtgccagcac aacaccattg cgccggtacc
     4381 ataacggtga atgacggttt cgctgtgatg ataatcgccg tgtggccact ggcaggattt
     4441 aatatggcgc aacagccagt cagacaatgc accagcacca ccagcagcac gaatcacccg
     4501 tgcgttactg aaaaacggca gcaatgtttt gtcttccacc agcggctggc gagcggcagg
     4561 aacgaccccg gacggcagat tacgcatgct tttcggttcc ggctccacca gtaaccgggt
     4621 attgtggaat accggcatgg attcacgacc gggcttaagg accaccagcc cgagttccgg
     4681 aaccagaaca ggtcgaagta atacccgcac gttacctcca gatccgttgc tggaatgtgc
     4741 gggacggacg cggtggctgt tcggagtaag ggagcctgac ggagattatc cagtgacggt
     4801 agtcgaggct aagggctttt ttaacctcgc atccgcgcct gcggtaacac tgaatgagcc
     4861 attcggcctg ttcttcagtg catgagggat gctggtacca gtctgactta aatgcgtgag
     4921 aataccgctc gtgcgtgtgg gcaagaacgg tcgaattatc atgattgtaa tattttgcgt
     4981 tgcgtgccat cggttttctc cggtggcacg gtgttactca gcgggagttc agccccgcgc
     5041 aagattgtag atgagtttat tcttctgaaa aagcagaaaa gccagctttt attccgatct
     5101 ctttcaatgc ctgtaatgaa gtgacaaact caccgtcgcg caagataaat ccgtccgtca
     5161 ctcgggcatc cacaaaatta attaacgcag ccccattttt tgcaaacaca caatgcggta
     5221 atgactaaca atatttccat tttcaacgca cacagcatag aggccatctt cacaaaaaat
     5281 tttacgcagt tcttcgatgt tcatcatcag aatccttcca gataagtagc actcccctgt
     5341 tcggggtcca tcccttttat ccctgcgcgc tacttaagta tttttgattc tattccggca
     5401 ccatccagaa cttcaaatgc gttgaaaata aaaacaaaaa cccgccgaag cgggttaagt
     5461 gcgggtgcgt tgaggatgcc tgccacatca gaggtggcga gggatttctc actcgccggg
     5521 tctcttactc ctcaggttcg taagctgtga agacagcgac ctccgtctgg ccggttcgga
     5581 ttcgtacctc gcagaggtct ttcctcgtta ccagtgccgt cactatgacg gttaaacaga
     5641 tgacgatcag ggcgattaac atcgcctttt gctgcttcat agcctgcttc tcctgtcaac
     5701 gcaaagcaga agtgtcacct tcggtgcgaa acagagatgt catgctttgg ttcagagaat
     5761 gcgtttgacc gcctcgctat atacttccga gcgttctctt ttcccaacag aaatcacgaa
     5821 aacgacaact ttctcgtcta taacctggta tacaaggcga tagcctgaag accggagctt
     5881 aatcttgtaa caatcaggca taccacggag cttgtttgct tcaatccggg gtgactcaag
     5941 tacttcaacc agcttctttt tcaactgttc acgtaccgtc gagcccagct ttcgccattc
     6001 ctttagtgcc cgctcgtcaa aatccagaaa atacgccatc agagttcatc cagcgtcaca
     6061 cgtactggct taggattacg aagccgttct ttcactatct ccacaagttc agcatcttca
     6121 tcactcagga gtgtctgttt gaacggcaag cgttcattgt cagcgatata ctcgagcatg
     6181 agacgaagcg cttcagaagg agttacaccc attttttcaa gcgcggcgta agaacgcgct
     6241 ttaagttcat cgtcaatacg caggttaatg ctacccatgt cttacacctc ttgtaattac
     6301 aaatgtcatt acaagtatcg cactacaaca tgcttagggc aagtcacgaa ggaagtcaga
     6361 aagtagtcgt aagaacggtg atcactgtcc gctttgtgcc aggagcagcc attgc
//
LOCUS       DAJTNM010000172         6372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTNM010000172 DAJTNM010000000
VERSION     DAJTNM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.172"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..423
                     /locus_tag="OO172_003803"
     CDS             <1..423
                     /locus_tag="OO172_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5513798.1"
                     /translation="GYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAV
                     VKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEK
                     GQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            434..1507
                     /locus_tag="OO172_003804"
     CDS             434..1507
                     /locus_tag="OO172_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5513799.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSSLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            1755..2795
                     /gene="pstS"
                     /locus_tag="OO172_003805"
     CDS             1755..2795
                     /gene="pstS"
                     /locus_tag="OO172_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5513800.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLEKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2882..3841
                     /gene="pstC"
                     /locus_tag="OO172_003806"
     CDS             2882..3841
                     /gene="pstC"
                     /locus_tag="OO172_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5513801.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3841..4731
                     /gene="pstA"
                     /locus_tag="OO172_003807"
     CDS             3841..4731
                     /gene="pstA"
                     /locus_tag="OO172_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5513802.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4823..5596
                     /gene="pstB"
                     /locus_tag="OO172_003808"
     CDS             4823..5596
                     /gene="pstB"
                     /locus_tag="OO172_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5513803.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPELRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5611..6336
                     /gene="phoU"
                     /locus_tag="OO172_003809"
     CDS             5611..6336
                     /gene="phoU"
                     /locus_tag="OO172_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5513804.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
BASE COUNT         1600 a         1502 c         1650 g         1620 t
ORIGIN      
        1 ggctatacgg tcaagacaca gctaaaccct tatgatgaaa atcgggtagc aatcagcgat
       61 aactatttct cgaagtcgaa tatagaactt gataataccg tcgttacgat ggttcccacg
      121 cgtggtgcag tggttaaagc ggagtttgtg actcatgtgg gttatcgcgt tctcttcaga
      181 gtgttaaatg caaatggtaa accggtacct tttggagcca ttgctgcgat acaagatgca
      241 agtttggcag attcaggaat tgtcggtgac cgtggcgaac tttatctttc tggtctacca
      301 gaaaaaggac aggttacgtt atcctgggga gaaaacgcct caacaaaatg catcttcaat
      361 tattcacttt cgacaccaga aagtgagagc ggattaattg aacagggtgt gacatgtcat
      421 taaaaggatt tttatgaaca agtatatcaa acagtggtgc ttttctgtgt ttatgctctc
      481 gttaagtagt gtagcacttg cagctcctaa aggtatctgt acccctgata atggcgtatt
      541 tcacagttcg cttgatttct cgggatatct tattaccgcg aacgaaaata aggttggaac
      601 gacatttaac accactgtaa ctaatggatc ctcatatctt ggacgctgtc attgtgatac
      661 tggtaacgta ggagaattcc cctatatcta ttatacgtca aaaataaacc aggcattaac
      721 ttatgctggg gttcactcta atattaatta ttatgatctt aatccgaacc ttgatgttgg
      781 gattgctata gatattcttg gggttgggta tgtgaatgca ccttttgaat atcatgctaa
      841 caacccgtct ggtaatacaa agtacaattg caaccgcatt gaacctttaa gtatatccag
      901 tggggctaaa gcgatagtat atttttatat caagaagaca tttgcaggaa aattgattat
      961 tcctgaaaca aagatagtga cattgtatgg aacaattagc cgtgacaccc cggtggatta
     1021 ctcacagccg atggctgatg tttatattcg gggcgatatt acagctccgc aaagttgcga
     1081 aataaataat ttaaagccag tttgttttga ttttaaagaa ataccagctg cagatttttc
     1141 atctgttgtt ggaagtgcgg taacaacaca taaaattact aaaacagtca ctattgagtg
     1201 tgaaaaccta gggatactga ataccgatga tatcagtacg tctttttatg ctaccgaacc
     1261 caatacggac aattcaatgg tcgtaacttc aaactctaac gtggggataa aaatttacga
     1321 caaaaataat aaggaaatca aagtgaacgg tggtgagttg ccaacagaca tggataaatc
     1381 aacagtgtat ggtgaaaaat caggtagtgt aactttttca gctgctcctg cgagccttac
     1441 tggagctcgc ccagccccgg ggcaatttac tgcaacagcg acgataactg ttgagattgt
     1501 gcgctaatag gtctgacaac gattgttcat cacatcgttg tcatctcacc catttcttta
     1561 tcacttttaa atgcatcatg acaaaaagat ggcatgtggg attttcatct tcacatttaa
     1621 aatcagtaag ttatttggta attcctttct gtcataaaaa tgtcatattc cttatatata
     1681 actgtcacct gtttgtccta ttttgcttct cgtagccaac aaacaatgct ttatgaatcc
     1741 tcccaggaga cattatgaaa gttatgcgta ccaccgtcgc aactgttgtc gccgcgacct
     1801 tatcgatgag tgctttctct gtgtttgcag aagcaagcct gacaggtgca ggtgcaacct
     1861 tccctgcgcc ggtgtatgcc aaatgggctg acacttacca gaaagaaacc ggtaataaag
     1921 ttaactacca gggtatcggt tcttccggtg gcgtaaaaca gattatcgct aataccgttg
     1981 attttggtgc ctctgacgcg ccgctgtctg acgaaaaact ggctcaggaa ggtctgttcc
     2041 agttcccgac cgtgattggc ggcgtggtgc tggcggttaa cattccaggg ctgaagtctg
     2101 gcgaactggt gctggatggt aaaaccctcg gcgacatcta cctgggcaaa atcaagaagt
     2161 gggatgatga agccatcgcc aaactgaatc cgggtctgaa actgccttca caaaacattg
     2221 ctgtagtacg ccgcgcagat ggctccggga cttccttcgt cttcaccagc tacctggaga
     2281 aagtgaacga agagtggaaa aacaacgttg gtactggctc taccgtaaaa tggccgatcg
     2341 gtctgggcgg taaaggtaac gacggtatcg ccgcgttcgt tcagcgtctg ccgggtgcaa
     2401 ttggttatgt tgaatatgct tacgcgaagc agaacaacct ggcgtacacc aaactgatct
     2461 ccgctgatgg taaaccggtt agtccgaccg aagaaaactt cgctaatgca gcaaaaggtg
     2521 cagactggag caaaaccttc gctcaggatc tgaccaacca gaaaggcgaa gatgcatggc
     2581 ctattacctc taccacgttc attctgatcc acaaagatca gaagaaacca gaacaaggca
     2641 cagaggtgct gaaattcttc gactgggcgt acaaaaccgg ggctaaacag gcgaacgacc
     2701 tggattacgc tagcctgccg gatagtgtag ttgaacaggt tcgcgctgcg tggaagacca
     2761 atattaaaga cagtagcggt aagccgctgt actaataaaa ctccaggccg ggtacggtgt
     2821 tttacgccgc atccggcatt acaaaatgac tttgtaaacg cgtttaactg aagagtaact
     2881 tatggctgca accaagcccg cttttaaccc accgggtaaa aagggcgaca taattttcag
     2941 cgtgctggta aaactggcgg cgctgattgt gctattgatg ttgggtggca ttattgtctc
     3001 tctgatcatc tcctcctggc cgagcattca gaaatttggt ctggctttcc tatggaccaa
     3061 agagtgggat gcaccgaacg atatctacgg ggcgctggtg ccgatctacg gtacgttggt
     3121 gacttcgttt atcgcgctgc tgatcgccgt cccggtgagt ttcggtatcg ccctgttcct
     3181 gactgagctt gcgcctggct ggctgaaacg cccgctgggt atcgccattg agctgctggc
     3241 agccattcca agtatcgttt acggcatgtg gggcctgttt atctttgcgc cgctgttcgc
     3301 cgtttacttt caggagccgg tcggcaatat catgtcgaat atcccgattg ttggcgcgct
     3361 gttctctggc cccgcatttg gtatcggtat cctcgcggca ggcgtgatcc tcgccatcat
     3421 gattattccg tacattgcgg cggtaatgcg tgatgtgttc gaacaaaccc cggtgatgat
     3481 gaaagagtcg gcctacggta ttggctgcac cacctgggaa gttatctggc gtatcgttct
     3541 tccgttcacc aaaaatggtg ttatcggcgg catcatgctg gggctgggcc gcgcgctcgg
     3601 tgaaaccatg gcggtgacct ttatcatcgg taacacctac cagctcgaca gcgcctcgct
     3661 gtacatgccg ggcaatagta tcacctctgc gctggcgaac gaatttgcgg aagcggaatc
     3721 cggtctgcac gttgccgcac tgatggaact gggcctgatc ctgtttgtga ttaccttcat
     3781 cgtcctcgcc gcatcgaagt ttatgattat gcgcctggct aagaatgagg gggcacgcta
     3841 atggctatgg ttgaaatgca aaccactgcg gcgctggctg aatctcgccg caaaatgcag
     3901 gcgcgtcgcc gcctcaaaaa ccgtattgcg ctgacgctct cgatggcgac gatggccttc
     3961 ggcctgttct ggctgatctg gattttaatg tccaccatca ctcgcggtat cgacggtatg
     4021 tcgctggcgc tgttcactga aatgacgccg ccgcccaata cggaaggtgg tggtctggcg
     4081 aacgccctgg cgggtagcgg gctgttaatt ttgtgggcca cggtattcgg tacgccgctg
     4141 ggcattatgg cggggattta tctggcggaa tatggtcgaa aatcctggct ggcagaagtg
     4201 attcgcttca ttaacgacat tctgctctcc gcgccgtcga ttgtggttgg tctgtttgtc
     4261 tacaccatcg tggtggcaca gatggagcac ttctccggct gggcgggcgt aattgcgctg
     4321 gcgctgttgc aggtgccgat cgttatccgc accaccgaga acatgctgaa actggtgccg
     4381 gacagcctgc gtgaagcggc ttatgcgctg ggtacaccga agtggaagat gatctccgcg
     4441 attacgctga aagcgtcggt gtccgggatt atgaccggta tcctgctggc gattgcccgt
     4501 attgccggtg aaaccgcgcc gctgctgttt accgcgctct ccaaccagtt ctggagcacg
     4561 gacatgatgc agccgatcgc caacctgccg gtgacgatct ttaagtttgc gatgagcccg
     4621 tttgcggaat ggcaacaact ggcctgggcc ggggtattga tcattaccct gtgcgtcctg
     4681 ctgctgaaca ttctggcgcg cgttgttttt gcgaagaata aacacggtta acactttgcc
     4741 ggatgcggcg tgaacgcctt acccggccct acggtaagcc tgattagcga aaagcatcag
     4801 gtacgatgag gaaaagattg caatgagtat ggttgaaact gccccgagta aaattcaggt
     4861 tcgtaatttg aacttctact acggcaaatt ccatgccctg aaaaacatca acctggatat
     4921 cgccaaaaac caggttacgg cgtttatcgg gccgtccggc tgcggtaaat cgacgctgct
     4981 gcgtaccttc aacaaaatgt ttgaactgta cccggagctg cgtgcggaag gtgaaattct
     5041 gcttgatggc gacaacatcc tgaccaactc tcaggatatc gccctgctac gtgcgaaagt
     5101 gggcatggtg ttccagaaac cgacgccgtt cccgatgtcc atctacgaca acatcgcctt
     5161 tggcgttcgt ctgtttgaga agctctcccg tgccgacatg gacgagcgcg tgcagtgggc
     5221 attgaccaaa gccgcattgt ggaacgaaac caaagataaa ttgcaccaga gcggttactc
     5281 tctctctggt ggtcagcaac agcgtctgtg tattgcgcgt ggtatcgcca ttcgcccgga
     5341 agtgctgctg ctcgacgaac cgtgttcggc gctcgacccc atctctaccg ggcgtattga
     5401 agagctgatc accgaactga agcaggatta caccgtggtg atcgttaccc acaacatgca
     5461 gcaggctgcg cgttgttccg accacacggc atttatgtac ctgggcgaat tgattgagtt
     5521 cagcaacacg gacgatctgt tcaccaagcc agcgaagaaa caaacagaag actacatcac
     5581 cggtcgttac ggttgattca ggagtgcgtt atggacagtc tcaatcttaa taaacatatt
     5641 tccggccagt tcaacgccga actggaaagt atccgcacgc aggtgatgac catgggcggc
     5701 atggtggagc agcagctttc tgatgcaatc accgcgatgc ataaccagga cagcgatctg
     5761 gcgaagcgcg tgatcgaagg cgacaagaac gtcaacatga tggaagtggc gatcgatgaa
     5821 gcctgcgtgc gcattatcgc caaacgtcag ccgacggcga gcgacctgcg actggttatg
     5881 gtgatcagta aaaccattgc cgagctggag cgtattggcg acgtggcgga caaaatctgc
     5941 cgtactgcgc tggagaaatt ctcccagcag catcagccgt tgctggtaag tctggagtcg
     6001 ctgggccgtc ataccatcca gatgctgcac gacgtgctgg acgcgttcgc gcggatggac
     6061 attgacgaag cggtacgtat ttatcgtgaa gataaaaaag tcgatcagga atacgaaggt
     6121 attgttcgtc aactgatgac ctacatgatg gaagattcgc gcactattcc gagcgtactt
     6181 accgcgctgt tctgtgcgcg ttcaatcgaa cgtatcggcg accgctgcca gaatatttgt
     6241 gagtttatct tctactacgt gaagggtcag gatttccgtc atgtaggcag tgatgagctg
     6301 gataaactgc tggcgggaaa agatagcgac aaataattca ccagacaaat cccaataact
     6361 taattattgg ga
//
LOCUS       DAJTNM010000173         6355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTNM010000173 DAJTNM010000000
VERSION     DAJTNM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.173"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            239..2671
                     /locus_tag="OO172_003810"
     CDS             239..2671
                     /locus_tag="OO172_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5513805.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDILCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2738..4198
                     /locus_tag="OO172_003811"
     CDS             2738..4198
                     /locus_tag="OO172_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5513806.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGK"
     gene            4207..5868
                     /locus_tag="OO172_003812"
     CDS             4207..5868
                     /locus_tag="OO172_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5513807.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6089..>6355
                     /gene="symE"
                     /locus_tag="OO172_003813"
     CDS             6089..>6355
                     /gene="symE"
                     /locus_tag="OO172_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5513808.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVC"
BASE COUNT         1882 a         1502 c         1459 g         1512 t
ORIGIN      
        1 ttcttgttcg aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgt
       61 ttcctgtaac ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa
      121 cagagatatc aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata
      181 gccaggctat tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat
      241 ggcggaactg aacctaagta acctgacgga agcagacatc attaccaaat gcgttatgcc
      301 agccattctc aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg
      361 ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga
      421 catcgtgctg taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa
      481 acatgaaatt ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc
      541 ttttgttttt gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg
      601 tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca
      661 aaaattctgc ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta
      721 ttacgacgat ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac
      781 cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg
      841 gaaaacctat accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg
      901 cattttgttc cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca
      961 gccatttggt acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat
     1021 tcacctcgcg ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca
     1081 agtcgcacca gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc
     1141 cgaagacagc gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt
     1201 aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt
     1261 ttacgtctac tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt
     1321 ccgtgttgat attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt
     1381 aaacggcgaa gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt
     1441 gatcgacgaa cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa
     1501 tccgatggat aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg
     1561 cgccctggtt aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat
     1621 caccggcgat gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc
     1681 gtacccggtt atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg
     1741 caaactggta gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg
     1801 tcgtggtaca cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa
     1861 aaaagccacc gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga
     1921 taccacacca gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat
     1981 cagcgaacat gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca
     2041 ggttaccgat accgatgatg tcggcccatt tccggaagaa gacgagaaga aaatccgcaa
     2101 gtttcacgtc aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc
     2161 cgacggtaaa ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga
     2221 atatgcctcg ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct
     2281 cattcacgag ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa
     2341 agatctcgac ccgttcgaca ttctttgcca cgtagtgtat ggtcagccgc cgttaacccg
     2401 caaagagcgc gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc
     2461 gcaagccgtg ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga
     2521 aagtattcag gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat
     2581 taaaaccgga tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga
     2641 aatctaccaa ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat
     2701 catgttgcag aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa
     2761 atcattacaa gatattatgc gcaaagatgc cggtgtggac ggcgatgcgc agcgtctcgg
     2821 tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact
     2881 ggagcaggat aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc
     2941 caacgcgcag ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc
     3001 ggcgttgaaa aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca
     3061 ggcgttcagc gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa
     3121 caagctgaac gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta
     3181 cgaacagatc cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg
     3241 cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga
     3301 cccggcctgc ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata
     3361 cgtgaagagc gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa
     3421 acagcttccg cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt
     3481 acaaattcgt cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga
     3541 tgtcattgtt accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt
     3601 tccggcagag atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt
     3661 actggcgaaa aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg
     3721 tgttaaaacc aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg
     3781 tttaccgaat ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac
     3841 caaaggtcag ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa
     3901 aaactacagc aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg
     3961 gggtaacgag gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag
     4021 cattgatgac gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga
     4081 aaccgtcagc catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat
     4141 ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaagtaggt
     4201 taactaatga gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg
     4261 caaacccgtt ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag
     4321 aaattgcgtg agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg
     4381 aatgatgaac cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg
     4441 aagcaaggca aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg
     4501 tttgaattgc cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct
     4561 gagtcatcta gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc
     4621 gagcaaagcc aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta
     4681 aataatccag ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat
     4741 tttgttgtcg gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc
     4801 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
     4861 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
     4921 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
     4981 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
     5041 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
     5101 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
     5161 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
     5221 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
     5281 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
     5341 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
     5401 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
     5461 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
     5521 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
     5581 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
     5641 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt
     5701 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
     5761 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     5821 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     5881 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     5941 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     6001 gatatatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     6061 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     6121 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     6181 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     6241 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     6301 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgt
//
LOCUS       DAJTNM010000174         6349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTNM010000174 DAJTNM010000000
VERSION     DAJTNM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.174"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            54..1201
                     /locus_tag="OO172_003814"
     CDS             join(54..291,291..1201)
                     /locus_tag="OO172_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5513809.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
     gene            1202..1459
                     /locus_tag="OO172_003815"
     CDS             1202..1459
                     /locus_tag="OO172_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513810.1"
                     /translation="MIRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLG
                     STAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            1499..2039
                     /locus_tag="OO172_003816"
                     /pseudo
     CDS             1499..2039
                     /locus_tag="OO172_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            2106..3524
                     /gene="dcm"
                     /locus_tag="OO172_003817"
     CDS             2106..3524
                     /gene="dcm"
                     /locus_tag="OO172_003817"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5513811.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3505..3975
                     /gene="vsr"
                     /locus_tag="OO172_003818"
     CDS             3505..3975
                     /gene="vsr"
                     /locus_tag="OO172_003818"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5513812.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3964..4884)
                     /gene="yedA"
                     /locus_tag="OO172_003819"
     CDS             complement(3964..4884)
                     /gene="yedA"
                     /locus_tag="OO172_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5513813.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            5057..5974
                     /locus_tag="OO172_003820"
     CDS             5057..5974
                     /locus_tag="OO172_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5513814.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGTIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6053..6235
                     /locus_tag="OO172_003821"
     CDS             6053..6235
                     /locus_tag="OO172_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5513815.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1578 a         1515 c         1790 g         1466 t
ORIGIN      
        1 cgggtttaac agacacctca gagtcattta agatggctta aagagaggtg cccatgagcg
       61 gtaagcgtta tcccgaagag tttaaaactg aagcagtcaa acaggttgtt gatcgcggtt
      121 attctgttgc cagcgttgca acacgtctcg atatcaccac ccacagcctt tatgcctgga
      181 taaagaagta cggtccggat tcttccacta ataaagaaca gtcagatgct caggccgaga
      241 tccgccgtct ccagaaagag ctgaaacggg ttaccgacga acgggacata ttaaaaaaag
      301 ccgcggcgta cttcgcaaag ctgtccgact gaggtacgcc tttatccgtg acaactcctg
      361 ttgctggcct gttcgcctgc tctgtcgggt gctggatgtt catcccagtg gtttttacgc
      421 ctggcttcag cagccgcatt cacaacgcca tcaggcagac ctgagactga caggacagat
      481 taaacagttc tggctggaat cgggatgcgt ctatggttat cgcaaaatcc atctggatct
      541 gcgtgacagc gggcaacagt gcggagtaaa cagagtctgg agactgatga aacgtgtcgg
      601 aataaaggct caggtcggat accgaagccc gcgggcacgt aaaggcgagg ccagtatcgt
      661 gtcacccaac aggctccagc gacagttcaa tccggatgct cctgatgagc gttgggtaac
      721 ggacataacc tacatcagga cccacgaagg ctggctgtat cttgccgttg ttgttgatct
      781 gttctcacgc aaaattatcg gctggtccat gcaatcccgg atgacaaagg acattgtcct
      841 gaacgcactg ctgatggctg tatggcggcg taatcccgaa aaacaggtgc tggttcattc
      901 ggatcagggc agtcagtaca caagccatga gtggcagtcg ttcctgaaat cacacggcct
      961 ggagggtagc atgagccgtc gcggtaactg ccatgataat gcggttgcag aaagtttttt
     1021 ccagttgttg aaacgtgaac ggataaagaa aaagatctac ggaacgcggg aagaagcccg
     1081 cagtgatatt tttgattaca tcgaaatgtt ttataacagt aagcgtcggc atggttctag
     1141 cgaacagatg tcaccgacag aatatgaaaa ccagtattat caacggctcg gaagtgtcta
     1201 gattatccgt ggcgattcat tgtgggcttt ctatacatgg tgtggctatt tcgtctgggc
     1261 aatggcgcga tacatctggg tgatgagccg gatccccgat gcccccgtgt cgggatttga
     1321 aagtgattta ggatctaccg cggggaagtg gttaggagca ttggtcggat ttctgtttat
     1381 ggccctggtt ggtgccttgt taggcggcat tgcatggtac acccgacctc gtcccgcacg
     1441 ttgcagacgc tatgaataaa aactgctaag gttgcgactc tttggcaacg gagcgcgtat
     1501 ggagttacaa cactggcagg cacagttcga gaactggttg aaaaaccatc atcaacatca
     1561 ggacgcggcg catgacctgt gtcattttcg ccgtgtctgg gctacggcac agaaactggc
     1621 ggcagatgac gatgtcgata tgctggtgat tttaaccgcc tgttattttc acgatatcgt
     1681 gagcctagcg aaaaatcatc cgcaacggca gcgttcttca atcctggcgg cagaagagac
     1741 gcgtcgcctg ctccgtgaag agttcgtgca atttccggcg gagaaaattg aggccgtttg
     1801 tcatgccatt gccgctcaca gtttcagcgc gcaaatcgcc cccttaacaa cggaagctaa
     1861 aatcgtccag gatgcggatc ggctggaagc cctgggggcg attggcctgg cgcgcgtatt
     1921 tgccgtttca ggggcattga gcttggcgct gtttgatggc gaagctccgt gccgaactgg
     1981 cgggggagaa tgaaggtatc gatcacaagg tgatagatgc gttttcaccc gctggctgag
     2041 cgcgtggccc taaacggcag taattatgtt aacctgtcgg ccatctcaga tggccggtta
     2101 aatctatgca ggaaaatata tcagtaaccg attcatacag caccgggaat gccgcacagg
     2161 caatgctgga gaaactgctg caaatttatg atgttaaaat gctggtggcg cagcttaatg
     2221 gtgtgggtga gaatcactgg agcgcggcaa ttttaaaacg tgcgctggcg aatgactcgg
     2281 catggcaccg tttaagtgag aaagagtttg cccatctgca aacgttgtta cccaaaccac
     2341 cggaacatca tccgcattat gcgtttcgct ttatcgatct atttgccgga attggcggca
     2401 tccgtcgtgg ttttgaatcg attggtggac agtgcgtgtt taccagcgaa tggaacaaac
     2461 atgcggtacg cacttataaa gccaaccatt attgcgaccc ggcgacgcat cattttaatg
     2521 aagatatccg cgacatcacc ctcagtcatc aagaaggcgt gagtgatgag gcggcggcgg
     2581 aacatattcg tcaacacatt cctgaacacg atgttttact ggccggtttc ccttgtcagc
     2641 cattttcgct ggctggcgta tcgaaaaaga actcgctcgg gcgggcgcac ggttttgcct
     2701 gcgataccca gggcacgctg ttctttgatg tggtgcgcat tatcgacgcg cgtcgtccgg
     2761 cgatgtttgt gctcgaaaac gtcaaaaacc tgaagagtca cgataaggga aaaacgttcc
     2821 gcatcatcat gcagacgctg gacgaactgg gctatgacgt tgccgatgca gaagataacg
     2881 ggccggacga tccgaaaatc atcgatggta aacatttcct gccgcagcac cgtgaacgca
     2941 tcgtgctggt gggttttcgt cgcgatctga atctgaaagc cgattttact ctgcgtgata
     3001 tcagcgaatg tttccctgca cagcgagtga cgctggcgca gctgctggac ccgatggttg
     3061 aggcgaaata tatcctgacg ccggtgctgt ggaagtacct ctatcgatat gcgaaaaaac
     3121 atcaggcgcg cggtaacggc ttcggttatg gaatggttta tccgaacaat ccgcaaagcg
     3181 tcacccgtac gctgtctgct cgttattaca aagatggcgc ggaaatttta atcgatcgcg
     3241 gttgggatat ggccacgggt gagaaagact ttgacgatcc gctgaatcag caacatcgtc
     3301 cacgtcggtt aacgcctcgt gaatgcgcgc gcttaatggg ttttgaagcg ccgggagaag
     3361 cgaaattccg cattccggtt tcggacactc aggcctatcg ccagttcggc aactcggtgg
     3421 tcgtgccggt ctttgccgcg gtggcaaaac tgcttgagcc aaaaatcaaa caggcggtgg
     3481 cgttgcgtca gcaagaggca caacatggtc gacgttcacg ataaggccac ccgcagcaaa
     3541 aatatgcgcg cgattgccac gcgtgatacg gcgattgaga agcgcctcgc cagtttgtta
     3601 accgggcagg gccttgcgtt tcgcgttcag gacgccagtc tgcccggacg cccggatttt
     3661 gtcgttgatg aatatcgctg cgtgatattt acccacggct gcttctggca tcatcatcac
     3721 tgctatctgt ttaaagtgcc tgcgactcga actgagttct ggctggagaa gataggtaaa
     3781 aatgttgaac gcgatcgccg cgatatcagt cgcttgcagg agctcggctg gcgcgtactg
     3841 attgtctggg agtgtgcgtt acgtaggcgc gagaagctga cggatgcggc gcttaccgag
     3901 cgtctggaag agtggatctg cggcgaaggt gccagcgcgc agatcgacac gcaggggatt
     3961 catttactcg cttgatgcat cctgaataac tggcgcgaca acgggtttgg ccgggaagag
     4021 atattttccc aacgtgacca gtaccaccgc gaaaacaatt acgccgagcg ccagccattc
     4081 aatcttcgac agcgtttcgc cacccagtcc cgtacccagc aagaccgcga ccaccgggtt
     4141 aacgtaggcg tagctggtgg cgagagccgg actgacatta cggattaaat acatataagc
     4201 gttaatggcg ataatcgaac caaacagcgc cagatagccg accgcaagga agcctgacag
     4261 ggaagggagc gccgtcagtt tttcacccgc aatcatcgat gcgatcatta acaccacgcc
     4321 tgccgccagc atctcaatcg cacccgccat catccctaca ggtaaggtaa tgcgcgagcc
     4381 ataaactgag ccaaacgccc agctaatcga gccgattaaa atcagaatcg cgccccacgg
     4441 attgccgctt aaatttccac cgctattgag catgatgatt ccggcaagcc caatggcaat
     4501 acccacccat tccagtttgc gcgttttaat gccaaacagg cgactgaagc acagggtaaa
     4561 gaggggcacg gttgcaatca ctacggcggc gatgccggaa ggaacatttt gatgttcggc
     4621 aaccgtcacc atgccattac cgacagccag caataacagg ccaatcagcg cggcattgag
     4681 cagcggacgt agcgggggaa gtttgtgtcc gcgcagtagc aaaaatgcca gcaataaaat
     4741 accggctgcc aggaatcgaa cgcccgccat cattaacgga ggccagcttc ccacgccaat
     4801 ccgaatgaca aaataggttg agccccaaat gatatacaac gcaaacagcg cgccaaaaag
     4861 cggtaacaac tggcggaaac gcataatccc tcacggcgga aataaaaagg tggttcatag
     4921 taaacgtgaa aatcattctg ctggctagag atataattgc acttgattgt taaataaatg
     4981 ttgacctgtg aagcaagtat cagagctgcc gtttttgctt catacttaca cccttcagca
     5041 ataaaaatga gagggaatgc ttttggccgg gagtagttta ctgacgttgc tcgatgatat
     5101 cgccacactg ctggacgata tctctgtgat gggcaaactg gcggcgaaga aaaccgccgg
     5161 tgtattaggg gatgatttat cgctcaatgc gcaacaagtt tcaggcgtgc gggccaaccg
     5221 ggaacttccc gtggtctggg gcgtggcgaa aggatcgctg atcaataaag tgattctggt
     5281 gccgctggcg ctgatcatca gtgcgtttat cccgtgggcg attacgccac tgttgatgat
     5341 tggtggcgcg tttctctgct ttgaaggggt agagaaagtg ctgcatatgc tggaggcgcg
     5401 taaacataaa gaagatccag cgcagagcca gcagcgtctg gagaagctgg cggcgcagga
     5461 tccgctgaag tttgaaaagg acaaaattaa agggacgatt cgtaccgatt ttattttgtc
     5521 tgcggaaatc gtcgccatca cgctggggat tgtggcagag gcgccgttgc ttaatcaggt
     5581 gctggtgctt tcaggcatcg cgctggtagt gaccgtgggc gtctacggtc tggtaggggt
     5641 tatcgttaag attgatgacc tagggtattg gctggcggaa aaatccagcg cgctgatgca
     5701 ggcattaggt aaagggttat tgattatcgc gccttggctg atgaaagcat tatcgattgt
     5761 cggcacgctg gcgatgttcc tcgtcggcgg cgggattgtg gtacatggta ttgcgccgct
     5821 gcatcacgct attgaacata tcgccgggca gcaaagtgca gtggtggcga tgatattacc
     5881 gactgtttta aatctgattc ttggatttat catcggcggc atcgtggtgc tgggagtgaa
     5941 agcagtagcg aaaatgcgcg gtcaggcaca ttaatactcg cactggctat tatgcaaaat
     6001 tcgccatctt caactaaggt gaacaagttt cactcgaaaa aaggaggcga gtatgagctt
     6061 tatggttagt gaggaagtta cggttaaaga gggcggcccg cgaatgattg tcactggata
     6121 ctccagcggt atggttgagt gtcgttggta tgacggttac ggggtcaagc gggaagcttt
     6181 tcatgaaacc gatcttgttc cgggggaggg gagtcgttct gcggaagaag tttgaaaggg
     6241 tatcattaac accaggccgg atcacatccg gcctggtgtc ccaatgcctg caatgaatga
     6301 tagtcgcgtc ttatcatgcc accattataa aattatcgct gttttgcgg
//
LOCUS       DAJTNM010000175         6306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTNM010000175 DAJTNM010000000
VERSION     DAJTNM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.175"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1208..2155
                     /gene="icsP"
                     /locus_tag="OO172_003822"
     CDS             1208..2155
                     /gene="icsP"
                     /locus_tag="OO172_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5513816.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2717..3089)
                     /locus_tag="OO172_003823"
                     /pseudo
     CDS             complement(2717..3089)
                     /locus_tag="OO172_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3333..3452
                     /locus_tag="OO172_003824"
                     /pseudo
     CDS             3333..3452
                     /locus_tag="OO172_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            3702..4568
                     /gene="ospB"
                     /locus_tag="OO172_003825"
     CDS             3702..4568
                     /gene="ospB"
                     /locus_tag="OO172_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5513817.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            4897..5637
                     /gene="phoN2"
                     /locus_tag="OO172_003826"
     CDS             4897..5637
                     /gene="phoN2"
                     /locus_tag="OO172_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5513818.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(5990..>6306)
                     /locus_tag="OO172_003827"
     CDS             complement(5990..>6306)
                     /locus_tag="OO172_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5513819.1"
                     /translation="KKADIALYLLSKFNFTKQDIAEMEKMNNNIYCNLYDVEYLLSKD
                     GANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRF
                     ER"
BASE COUNT         1996 a         1170 c         1222 g         1918 t
ORIGIN      
        1 tttatggtgt aattgtttga ttgaatactt ccggggattt cagtatgaaa tgaagtatat
       61 ttaatatact ttacaaaaag tttgtattag tggaataagg acaatagctt atccaaactg
      121 ccaagcctct ttattataag taagatctgg cactggaata ttctcaaaag gactaacatc
      181 atcaccacga ccttcaacca accattttat tgcatttaaa tcccagaata gttcagtagt
      241 ggtcaatttg ggaaacgcac aatattgtac ggttacgtta tcgcaataga gtgaactccc
      301 gccctatacg gcgttttcaa ggattagtcc ttaatcggac aaccacatta aagcataata
      361 aataattaag ctttaataat atactataga tacagttcta ttataaaaaa tactatttta
      421 ggtgatgtat taaattagta gaatatttat tcttttataa aagcaccaat gaattgtcca
      481 agtaaacatg aggtaaaatg tgtagaggat aactgagttt ggtcacttta actgtattag
      541 tcgcagtctg actctcgact ttaaaaggat gggaattacc tgtactgata gtgagaaagc
      601 tttccattga aataagtcaa ataaaagttg atggcttcag tgctaaagtg aggtgcccgc
      661 tagccgtatt cactcgaatc ggaattcaat gtattgcttc tgccatttcc ataatatttc
      721 acgggaaaaa caaaatctga aaaactatac ttaagtgttg tattaagtca gttgagatac
      781 aaatacatat ttatcagcac acgttacgtc aaatacagga ggttgtcatg ttggtactga
      841 aaggcacgtt ggctattgca gccgtgatga caatttattg tcttatcgtt gttcttatgg
      901 aacgcttttc tgattgattt catattggcg agagaacaag aaggtgagta gaattgtcgc
      961 ggaatcctga aactatcagc ctcaggtatg aacaccaaaa ggtaggtaga gaaatctatg
     1021 gcccccgtta ctttattcgc acaatgaggt atttttttat ttttacaaaa gttcaataaa
     1081 tattataata aaagatatgt tcttggtaac ataaacaaat atttaattaa aataatgctt
     1141 ggcattgacg tttatcttat aaagtaagaa gatcatggac atttcaacca aaaaagtaga
     1201 gttctcgatg aaatcaaaat tccttgtact tgcactttgt gtacctgcga tctttactac
     1261 acatgctacc actaactatc cacttttcat accggacaac atcagtacgg atattagtct
     1321 aggatctctg agtggcaaaa caaaagaacg cgtttatcat cccaaggaag gggggagaaa
     1381 aattagtcaa ctggactgga aatacagtaa tgcgactatt gttagaggtg gcatcgattg
     1441 gaagctaatt ccaaaagtgt ctttcggagt ttccggttgg actactttag gtaaccagaa
     1501 agcaagcatg gttgataaag actggaacaa ttccaatacc cctcaggtat ggaccgacca
     1561 gagctggcat cccaatacgc atctccgtga tgctaacgaa ttcgagctga atcttaaagg
     1621 ttggttatta aataatttgg attatcgact tggactaata gcaggttacc aagagagtcg
     1681 ttacagtttt aatgcaatgg gagggagtta tatttatagt gaaaacgggg ggaacaggaa
     1741 taaaaaaggg gcacatccta gtggtgaaag aacaataggt tacaaacagc tcttcaaaat
     1801 accttatatt ggattaactg ctaattaccg ccatgagaat tttgagtttg gagcagaact
     1861 gaaatatagt ggttgggttc tttcatctga tacagataaa cactatcaga ctgagacaat
     1921 ttttaaagat gaaataaaaa accaaaatta ctgctctgtt gctgcgaata ttggatacta
     1981 tgtcaccccc agtgcaaaat tttatataga aggctccaga aattacattt ctaataaaaa
     2041 aggtgataca tctctttatg agcaaagtac caatatatct ggcaccatta aaaatagtgc
     2101 aagtattgaa tatattggtt ttctcacttc cgcaggtata aagtatattt tttgaattgg
     2161 ttcaccgagt tgattacgtt ttcatggcta aaaattgccg cccattgtga gtcatattcg
     2221 ccctgacttt ccagaaccat acgaactgcc cgttgacgga cttcggggga aaaacgtgta
     2281 tttttagtca ttctgtttac ctctttctca gggcgtttag tctccaggat ttccggggcg
     2341 gttcaaaaaa ctcttcgttg attactgtgg acttactgtt ggcattaccg accctgagac
     2401 cggagaaata agaaccgctt agatcatcgt ggctgttctc ggggtatcaa gttacacatg
     2461 ggcagaggct acccaaggtc tactattatc gcctgaaagc gctgatggaa cagtgctatc
     2521 agggacatgc tgatgaaaat acctaaataa tcagccagca ctctatcttt ccaaatccac
     2581 agcatagcaa agagagcaaa agagcctgta aattcagaaa ttaaggaaac gcctcttggt
     2641 gcacataatc tatcttatgt taaatgaaat ctaatttctt gattatatgc tggatcgttc
     2701 gtgggtggct ttgcccctat atttccagac acctgttatc attggcccgc tgccgcagat
     2761 attcccgtgg cgagcgataa tccagcgcac tatgcggatg ccattcgtta taatgctcga
     2821 atgcctctgc aaggttcttt gctgccgtta acccgtcagg tttgggcatg atactgatgt
     2881 agtcacactt tattgttttc acgaagctct ttgctattct gttactctcc ggactccgca
     2941 ccgccgtgtt cttcggttca acaaagtccc aacatccggg tgaactggcg tgtttcatta
     3001 gcccggtagc atgaaccatt atccgtcagt cactccactg gagacgacgg aagatcgttg
     3061 ccgaagcggt gttccaccgc tcccagcatg tcgccaatgg gagagcatct acggtctgat
     3121 tgaactgttt attcaaattg tccaccgtct gtcagtacgc tctgaacgtc ggctggttaa
     3181 aacactgctc gctgattttc agagggttca cggtaaaaca gcacttttat tccggattgc
     3241 ggaagcggct ctgaataatc cggatgggct ggttaaagag gttgtgtacc atctgcatat
     3301 tgttgaacca gatcgcagcg gtaagagaag ttcatcatac ttggcgcagt tacgcgatgt
     3361 ttctgctaga ggtgatgccg taaagaacgg cagaacatta ccggaacaag atagcggtct
     3421 acctgcactg gtatcagacc cggggcttcc ccggatgatc tccacggtac tttaggtact
     3481 gtgtctatat tatgtaggta ggatcaatta actttgtagt tgaaattctt tcattgaaat
     3541 aatgaataaa aaagacaaac cgcattaaac aatgccagaa tataattgca tttttgttcc
     3601 gtttttttaa atcatagtat acaaagaagc ttgcatacta tatgaaaata taacaccctt
     3661 tactatgatg tttataaaac aatatatgga gtcatgtagg tatgaattta gatggtgtta
     3721 gaccatactg tagaatagtc aataaaaaga atgaaagcat atcagatatt gcatttgcac
     3781 atataataaa aagggtaaaa aattcatcat gtactcaccc aaaagcagca ttggtttttt
     3841 taggagagaa aggtttttgt gatagcaatg atgttctatc tattatggga caacaaatac
     3901 caagagtatt taagaacaag atgttatatg attatgtttt taaaaatgaa aaaagtaaaa
     3961 atgattttct aaaaatggct gaatcatggc taccacagag tgaaccaata gtaataaata
     4021 atgatgatga cgcattgaat gctgctgctt atttttctgt aaaaaaagcg aaaataaaaa
     4081 cagtaaacga tactgatttt aaagagtata ataaggttta tattcttggg cacggtagtc
     4141 ctggttctca tcaattaggc cttggttcgg aacttattga tgtacaaaca atcatttcaa
     4201 gaatgaaaga ctgtggtatt ctaaatgtga aagatatccg ttttacttca tgcggctccg
     4261 ctgataaagt ggctcctaaa aattttaaca atgcccctgc tgaaagtctt tcttgtatcc
     4321 ttaactctct gccttttttt aaggaaaaag aatctttgct agagcagata aaaaaacacc
     4381 ttgaaaacga tgagtcattg agtgatggtc taaaaatatc cggctatcat ggatatggag
     4441 ttcactatgg tcaagagctt tttccctact cacattatcg ttcaacttca attcctgctg
     4501 atccggagca tacagtaaaa agaagctctc agaaaaagac ttttattatt aataaagaac
     4561 tggattagta taaaattttt aacctatagt taattgtttg catttaagat gaagttattt
     4621 tatctttagt agaaaagtgc ttccctatga gggaaaatca caaatcatca taatacagag
     4681 acaaaacgat acgaacaaat agataaaaaa catcattctt ttaccacatt atttttcgtg
     4741 aatatgaaaa ataatgttat tactttaata taagactatt ttttgttttt ccatcactct
     4801 gttcaaattt ttccgcatga cttgtgtttt ttgtaacaca gctcgttttt tacagctgac
     4861 caaaatcatc aattaattat gctaaggaaa taaattatga aaaccaaaaa ctttcttctt
     4921 ttttgtattg ctacaaatat gatttttatc ccctcagcaa atgctctgaa ggcagaaggt
     4981 tttctcactc aacaaacttc accagacagt ttgtcaatac ttccgccgcc tccggcagag
     5041 gattcagtag tatttcaggc tgacaaagct cattatgaat tcggccgctc gctccgggat
     5101 gctaatcgtg tacgtctcgc tagcgaagat gcatactacg agaattttgg tcttgcattt
     5161 tcagatgctt atggcatgga tatttcaagg gaaaataccc caatcttata tcagttgtta
     5221 acacaagtac tacaggatag ccatgattac gccgtgcgta acgccaaaga atattataaa
     5281 agagttcgtc cattcgttat ttataaagac gcaacctgta cacctgataa agatgagaaa
     5341 atggctatca ctggctctta tccctctggt catgcatcct ttggttgggc agtagcactg
     5401 atacttgcgg agattaatcc tcaacgtaaa gcggaaatac ttcgacgtgg atatgagttt
     5461 ggagaaagtc gggtcatctg cggtgcgcat tggcaaagcg atgtagaggc tgggcgttta
     5521 atgggagcat cggttgttgc agtacttcat aatacacctg aatttaccaa aagccttagc
     5581 gaagccaaaa aagagtttga agaattaaat actcctacca atgaactgac cccataaagc
     5641 tggacagcct gtatcaggct atggagggcc catagacaaa tctaccctat atgagcatag
     5701 gaggagtcta tgggcacacc acgttttact cctgaattta agggattact ggaaaggctg
     5761 ggacatatcc ctccggcaga agcagaaaaa gcttattatg ctgccatcgg aaacgatgat
     5821 ctggcaacct gagttcacag ataaaacatt ctctaggaaa ctcggggcgg ttccgttcac
     5881 cacatgcaat gtggtgttgc aggggaacgg tctgcaccat cccctatatc gatcaatata
     5941 acaaaaatga caacttcaga ttcagggcac aacctaaata tattttaggt cacctctcaa
     6001 atcgtttgcc tgataccgct ccatttttta ataaaaaatc aatcattttt gaatctttgc
     6061 ttttcattgc attgtcaagc atagtgtccc cactatttgc tttttgaaat tttttgttta
     6121 catcaaccaa tccattattg ataaaatatt ctagcacttt atagttggca ccatctttac
     6181 taagtaagta ttcaacatca tacagattac aatatatatt gttgttcatt ttctccatct
     6241 ctgcgatatc ttgttttgta aaattaaatt tagatagtaa ataaagtgct atatcagctt
     6301 ttttat
//
LOCUS       DAJTNM010000176         6301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTNM010000176 DAJTNM010000000
VERSION     DAJTNM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.176"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            93..272
                     /locus_tag="OO172_003828"
                     /pseudo
     CDS             93..272
                     /locus_tag="OO172_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            798..1976
                     /gene="ltrA"
                     /locus_tag="OO172_003829"
     CDS             798..1976
                     /gene="ltrA"
                     /locus_tag="OO172_003829"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5513820.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDVTRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2059..2238
                     /locus_tag="OO172_003830"
                     /pseudo
     CDS             2059..2238
                     /locus_tag="OO172_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2268..2750)
                     /locus_tag="OO172_003831"
                     /pseudo
     CDS             complement(2268..2750)
                     /locus_tag="OO172_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3006..3986)
                     /locus_tag="OO172_003832"
     CDS             complement(3006..3986)
                     /locus_tag="OO172_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5513821.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3986..5185)
                     /locus_tag="OO172_003833"
     CDS             complement(3986..5185)
                     /locus_tag="OO172_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5513822.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5507..5845)
                     /locus_tag="OO172_003834"
                     /pseudo
     CDS             complement(5507..5845)
                     /locus_tag="OO172_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5912..6301)
                     /gene="istB"
                     /locus_tag="OO172_003835"
                     /pseudo
     CDS             complement(5912..6301)
                     /gene="istB"
                     /locus_tag="OO172_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT         1709 a         1449 c         1505 g         1638 t
ORIGIN      
        1 cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca gcctgagttc
       61 acagataaaa cactctccag gaaacccggg gcggttcagt tgctgaactg ctggaaccgc
      121 tgtatgacat cctgcgccag tatgtgctga tgcccggtaa agtccatgcc gatgatatcc
      181 ccgttccggt ccaggagccg ggcagcggta aaacaaggac tgcccggctg tgggtctacg
      241 ttcgtgatga ccgtaacgcc ggttcggaaa ttgcagcata agttatccat gctctgaggt
      301 gggtggaggt gggccctccg gtcagggttt acaggagcac tgaccaaacg acccggtgct
      361 gcttcattct aaggaatgag gtggtgagcg actcgggaaa aggcagagat ctactgtcac
      421 gtggggttcg ggaagctgaa cgaaagtaaa ctgtccgatg aagcatcgaa atcttagatg
      481 atgccaaaat ggggccgtac agccgtcccc tgataagcgc agaagacacc tgcttactga
      541 ctgcgcggca tccggtgtga aggcagcagg atcccgggcc agaccatctc agggaacgtg
      601 agaacctgtt tccggcacgt atcgggaaac aggggcggac ccattcgtag tagtgatgaa
      661 agatctgtaa tgggtctgga gcgaagggat ggggatcaca ttcattgcag aagacacaac
      721 ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt gaaataccaa aagcactggt
      781 ctgggcatca tatctggatg tgcgtcgcaa caagggagcc ccccggatgc gacgggcaga
      841 ctttgaaaat gttcgaccag caacgtgatg gcaatctata caagatctgg aaccggctat
      901 gttcgggaac atggtttccg ccaccagtgc tggaaaaacg gatcccgaag tcgaatggca
      961 aggaacgcat tctggggatc ccaacagtat ccgatcgaat tgctcaggga gcgataaaac
     1021 ttttcatgga agaaaagctt gacccgattt tccatgctga ttcatatggt taccgaccag
     1081 gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg gtgctggcgt tatagctgga
     1141 tactggaagt ggacatcagt gcgtttttcg accatgtgag acatgatctg gtgctcaaag
     1201 ctctggaaca ccacgggatg cctaaatggg tcatcctgta ctgtcgacgc tggatggaag
     1261 cgccaatgca gagttgtgaa aatggagaat taataacccg aacacgaggc actccgcagg
     1321 gcggggtcat tagtccgcta cttgctaact tgttccttca ctatgcattt gatttgtgga
     1381 tggaaagaga atatcggggg gtaccgtttg agaggtacgc tgacgatatt gtagtacatt
     1441 gttcacgaat gagtgatgta acaaggctaa agaacaggtt atcggagcgc ttctcggaag
     1501 tcgggctggt cctgaatgca gggaaaacga atatcgccta cattgacacg tttaaaagga
     1561 gaaacgtcgc aacgagtttc acctttctcg gatatgactt caaagtgcgt acgctgaaga
     1621 atttcaaagg cgaacggtac cgaaaatgca tgccgggtgc gtcaaatgca gcaatgcgca
     1681 aaataacaga aacaatcaag aagtggcgta tacatcgctc aacagctgag agtttgctgg
     1741 attttgcgag gcgctacaat gcgatagtga gaggctggat cgagtactac ggaaagttct
     1801 ggtccagaaa tttcaactat cgactgtgga gtgcaatgca gtcacgtctg ctcaagtgga
     1861 tgcagtctaa atacagactt tcgaaccgga aggctcagcg aaagctgacg ctggtaagga
     1921 aggagtatcc gaagctattt gttcactggt atttactacg tgcatcaaat gagtgatcaa
     1981 gagccgtatg acgcgagagt gtcacgtacg gttccgtgag cagccgggga gtgaaattct
     2041 ctccggctga ctcgactcgc gggttccgac agcgatgacg aacatgcggc ggtgttgtac
     2101 tcgctgatcg gcacatgccg tctgaacaat gtggaaccag aaaaatggct gcgttacgtc
     2161 attgagcata tccaggactg gccggcaaac cgggtacgtg atctgttgcc ctggaaagtt
     2221 gatctgagct ctcagtaaat atcaatacgg ttctgacgag ccgcttactt ttggcgtaat
     2281 tctgctgcgc ctttactgaa cacaatgccg tattcagcaa gaagtccacg aatgtgattt
     2341 atcagttgtg tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt ctggacgtcc
     2401 tgttgctcca gagtttttat tggtacgggt cgtatagttc catgttggca ggcctcagct
     2461 attgcctgtg cgtcgttata atcatttttc tggcaccgct gataagcttt aacatgttgt
     2521 gccggaagca atattacatc atggtcgagt ttcctgattt cccgggccca gtaatgagat
     2581 gccccgccag cctccatcgc aacagtcatt ttaggctgtt gcacctgcca gttaagcagt
     2641 tgattgcgag aaagtttggt ctttcctgcg ggtttgccat gagcattgat actgaaaaga
     2701 taaaagttcg ttttagccag atcaatgccg gtgattttct gttcagacat ggtatcccgc
     2761 ttacttttgt ggttaaagtt tatccatcat ggcgcaattg aaggctgccg tgaaagaggg
     2821 ggaagactat ctcatcggtt actgttaaac ggctaagaga aactatcttt ttcaagcaaa
     2881 gaagatacgg tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa acgctaataa
     2941 aagcaaaatt tcttttaaaa cagccaatta aaaactggtg agttgctggc gaaaaacttg
     3001 aattttcaca gcgaattcag attatctttt agtacaagag caatcatacg atctaactta
     3061 tcctgaactt ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc ctttactcgt
     3121 tttcgagcaa atttatcttt actatcaaat ttccataaca aagttacaac cgctttatcc
     3181 tttactcctg aatcaagcaa tgaagacatt tccttggtta taagccgcat aatactgttt
     3241 ttaacctcgt cttcagaaag attgtcatca gcagtaataa gctcaatact cggcgagata
     3301 ttcttcacaa cttcatcaat agatatatca cccagcctca gtcgttcact taattcagcc
     3361 agttgcctgt aatcggcgta tgtcagctca gactgaacag gaaaaagaga cacaaaatct
     3421 ttaggtacgg acgctgcttg gagggcacga gtcaccttag ctgccgacaa tccctctctt
     3481 tccgcaattt gcttctggtt cataccggcc tctttgagac gtactaaacg caacccaatt
     3541 tcacgaatat tatgttcaag tgacgtctgc aaatctttcg ccagatgctg agcttcagaa
     3601 acagtaagct catcatccgt tacaagtaca cgtaatccaa ccttgcatag caaagctgct
     3661 gcccgacgac gggaaccatc caaaatctca ataagatcgc cagtccgaat cccaatacat
     3721 ggatagaatt gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc cggcgtcagg
     3781 gctgtctgtt cgcggccatt cacctcttca accacaaaag tttgagtgtc cactttgtca
     3841 tgatcaacct tgatctcagt aaattttgcc tttcggccag tattgagggt aaatacatga
     3901 actggagccg atatttcttc agtattattc aggatatttg tattcagcgt acggccaata
     3961 gtcggtcggt gctttctgtt ttccatcagt agttggccct tataaattca atacgatcaa
     4021 aaactgcttt agcgaaatct tctgctgcca tacgtgcatt tttcaaagct tcaccactgc
     4081 caacataagt aacaggattc gcagatatca cggtatcaaa cgactcgccg cttctctcaa
     4141 aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc tcttttgtca
     4201 ggctatggca gtatttatga tctgacttat tcgccagttt cgacatgaag ccaatatttg
     4261 cctgcaaacg gcatgaacat ccatcctgtt caataatctg tacaagttca ggtaaacgag
     4321 ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt gtaaacatga
     4381 tatctgcggc agcaatggca tttttcaaaa atgcgtccag atgcgggcct gtatcaataa
     4441 ggatgaagtc gaaatcatgc ttcagcttat caataatatt ctcacgcaat atcgcgtgtt
     4501 tgttttgtcc taatagatgc tcctcgcaca gagtatccca gtttgatgca ataaaggcgt
     4561 cgtcaataga agccggcata acataaacgc cagggataac tgacggaacg ataaaatctt
     4621 ccagtaattc ctcacgactc acgttctgca acatagcctg cggtgcggtt gtatcaacca
     4681 gccccaccgc atgcaagtag ttcagaaaca tagtggctga tgactgggga tctaaatcaa
     4741 ggagcagaat acgtaaatct tctgacagca ggtgaggatg agcacgcagg gcgtgagcta
     4801 cggaaacact actcacggtt ttagacacac cgccttttag actaccgatg aatatggaat
     4861 atgcctcact ataacgatcg cgatatttag gaatgccacg atgtgcatag atatcgataa
     4921 tgttctggat ggttaatgca tattttgtcg cggttcctgc tgggcgtttt tcaaactgat
     4981 agccctgagc ctccatctca ccgactgcac catccacgct tgcgcgcgtt aattttggaa
     5041 gcttagctac agcagacttt gaatagactt gataaaaaac atcagcctga aactcctgct
     5101 tttgctcctg aatctgtttt gttagggcga gcaacatttt atctgcacgc tgcgcaacct
     5161 tacttaactg ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt taacttatta
     5221 caaaaaaaac tgcattagca gggtaatgca tgcaagtaca tattgtacat gcttatatta
     5281 aaaatgcgcg cattgctgat ttattaatca tcggaatgaa tattactgta agatagtatg
     5341 cctttccggg agaaagacgc tgatttcccc cgaataatcc agttcaccac cacactccgg
     5401 acaacagctt tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt ccggtaacgg
     5461 acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag tagaacatgt
     5521 gcttttcaat gaaggagccg atgacttttt cgtggatttc cacggaacgc gagaagcaga
     5581 ttgttttacg tgccagccgc ttgatgcggg tgcagagggt ccggttgtta cgttcaatac
     5641 gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg cacttctctg gcgtagctgc
     5701 cccagtcatc gctggtcacc atgccgatac agaacggagt caacagggct aacagttccc
     5761 gacaggtctc atcattccgt ggcccgaaag tgtaagccaa cacaccgcca gttttagtgt
     5821 tgtacgcata ccagagccag tgttgtgtca acgacggatg aaaagtgctc cacttatatc
     5881 tccaccaacg gcccaatatt gatccaccgt tttactcagg attagcttct gctataaccc
     5941 cggcctttcg tttctgtctg agtcgatagc tttctccttt gatttgaacg acatgtgagt
     6001 ggtgtaagat acggcccagc atcgctgagg tcagtgctgc atcaccggcg aacgtttgat
     6061 cccactgccc gaacggtaga ttggatgtca ggatcattgc gctcttttcg taacgtttag
     6121 cgatgacctg gaagaacagt tttgcttctt cctgactgaa cggcagatag cctatttcat
     6181 caatgatgag caggcggggg ccattactcc acgctgaagc gtcgttttat aacggccctg
     6241 acgttgtgcc gtagataact gaagtaacag atctgctgct gttgtgaagc gaactttgat
     6301 a
//
LOCUS       DAJTNM010000177         6242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTNM010000177 DAJTNM010000000
VERSION     DAJTNM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.177"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..312
                     /locus_tag="OO172_003836"
     CDS             <1..312
                     /locus_tag="OO172_003836"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5513823.1"
                     /translation="VAMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKT
                     HDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNV
                     R"
     gene            complement(388..852)
                     /locus_tag="OO172_003837"
     CDS             complement(388..852)
                     /locus_tag="OO172_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5513824.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OO172_003838"
     CDS             complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OO172_003838"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5513825.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="OO172_003839"
     CDS             complement(1721..2410)
                     /locus_tag="OO172_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5513826.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OO172_003840"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OO172_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5513827.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OO172_003841"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OO172_003841"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5513828.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="OO172_003842"
     CDS             complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="OO172_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5513829.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1581 a         1693 c         1548 g         1420 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag gatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcaattgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgtggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcagcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatgg tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
     5881 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcaggttt
     5941 ttctctttgt acctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
     6001 atgtaacgca gataaacaca tcccgattga attattcata agactattca caagcattat
     6061 gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta atgaaaaatc
     6121 acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca aaaaatataa
     6181 catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac acggagttca
     6241 ac
//
LOCUS       DAJTNM010000178         6143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTNM010000178 DAJTNM010000000
VERSION     DAJTNM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.178"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(1..367)
                     /locus_tag="OO172_003843"
                     /pseudo
     CDS             complement(1..367)
                     /locus_tag="OO172_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(707..1261)
                     /locus_tag="OO172_003844"
                     /pseudo
     CDS             complement(707..1261)
                     /locus_tag="OO172_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1404..1526
                     /locus_tag="OO172_003845"
                     /pseudo
     CDS             1404..1526
                     /locus_tag="OO172_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1788..2354)
                     /gene="ipgB2"
                     /locus_tag="OO172_003846"
     CDS             complement(1788..2354)
                     /gene="ipgB2"
                     /locus_tag="OO172_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5513830.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2636..2950)
                     /locus_tag="OO172_003847"
     CDS             complement(2636..2950)
                     /locus_tag="OO172_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513831.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3028..3138)
                     /locus_tag="OO172_003848"
                     /pseudo
     CDS             complement(3028..3138)
                     /locus_tag="OO172_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3462..4139)
                     /gene="ospD1"
                     /locus_tag="OO172_003849"
     CDS             complement(3462..4139)
                     /gene="ospD1"
                     /locus_tag="OO172_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5513832.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4510..4647)
                     /locus_tag="OO172_003850"
                     /pseudo
     CDS             complement(4510..4647)
                     /locus_tag="OO172_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4729..5027)
                     /locus_tag="OO172_003851"
                     /pseudo
     CDS             complement(4729..5027)
                     /locus_tag="OO172_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5616..5775)
                     /locus_tag="OO172_003852"
                     /pseudo
     CDS             complement(5616..5775)
                     /locus_tag="OO172_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5779..6143)
                     /locus_tag="OO172_003853"
                     /pseudo
     CDS             complement(5779..6143)
                     /locus_tag="OO172_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1652 a         1356 c         1248 g         1887 t
ORIGIN      
        1 tgacagtgtt gttgcgcctt gctgccaacg aagctccact gcccatcaag ttcacagata
       61 agggcaacat cagcgtgagc gactggcgca gatgttatcc gccggggcgg gagcttttta
      121 atgtccgtat gacggtattg atgccgattt tcagcgtacg ggcagtatcg cgaactccgg
      181 caccgttgaa agccatttca gtgatttggt ttttaactcc cggtttacgg gcctcgtaac
      241 agtaagtgag ctgaaacacc cggtgacagt cacggcaacg gaatcgatca tgtccttttg
      301 ggttctgccc gtgacggtaa acctgaacgg actggcaacg ggggcaatga aagttaactg
      361 tgaccatgaa agaaccttaa aagcgggtat tataacccct aattcaacta attggaggca
      421 ttgtcatttt taatctatgg atacacttaa aaatcctctc accttacgtt aaaaaccttc
      481 tcatgtttag agttttttat tatgtttatc ataatgatat gacgatctga gtttttaaaa
      541 tcaagaagaa aagataagtt ttttaaatgc acagtgcctg tggataactt tgcgtaaatc
      601 cggaggacag atcactctga agcaagacaa cagcatctga aaacaatatg ctcacgtttc
      661 aaccacaggg cgtaagtgat cctacccacg taatatggac acaggcctaa gcgaggttct
      721 ggttttcaaa ttgttccgga ctgaggccgc cacaccaact gtgccgtcgc cagcgattgt
      781 aatcacattc gatataatta aacactgttg cccgcattat ttcccggctg ataaagtgtt
      841 ctccgtggag acattccact ttcagcgaat gaaagaagct ttccacgcag gcattatcgt
      901 agcagcagcc ctttgcgctc atactgccac gcagattatg ccacttcagc agcgcctgat
      961 aatctgctga acagtactga cttcctcggt ccgagtgaac gataacgttc cggggtctct
     1021 tacgccgcca cagcgccatc tgcagggcat cgcaggccag ttgcgccgtc aggcgtggtg
     1081 acatggacca gccaataacg gcacgtgacc acaggtcaat gaccacagcc agatacagcc
     1141 agccttcatc tgtacgtaag tacgtgatgt ctcccgccca cttctggttc ggaccactgg
     1201 cgtaaaaatc ctgctccagc agattttctg acacaggcag gccatgtgcg cggtaactga
     1261 ccgtattgct gggtaagatc aatttgctca ctggtattta ctacgtgcat cgaatgagtg
     1321 atcaagagcc gtatgacgcg agagtgtcac gtacggttcc gtaagcagcc ggagagtgaa
     1381 actctctccg gctgactcga ctcgcaggtt ccgacagcgg tgacgaacat gcagcggtgt
     1441 tgttctcgct gatcggcaca tgccgtctga acaatgtgga gccagaaaaa tggctgcgtt
     1501 acgttattga acatatccag gactgaccgg caaaccgggt acgcgatctg ttgccttgga
     1561 aagttgatct gacttctcag taaatatcaa tatgattctg acgagccgct tacctttgag
     1621 cagagaaagg ggagagggat cccacatcca tgagctagga aaatacaaaa actggctata
     1681 caacaattca atatattagg acttacttgc gaggagaaat atagcaccgc tcaggcaatt
     1741 cctgggtatt tacttttctt agtttcttta ctcgggatgt tctgtcatca gaaaggcgat
     1801 tctaaatttg taatatagtc actcatattc ccacgtaatt tctttagatc aatatattgc
     1861 acatttttct caaaaaactg atcggaaata agatttgcaa cattgcttgt tgtatcattt
     1921 ttaacgcttc tattcatccc ttcacaaagt tcgtccattt tctttttgaa ataactgctt
     1981 cctgatacaa gatgatttat ggaactttgt gcagcattga tgtctagaga aatgcgataa
     2041 tctttctcaa cctgtttaaa tatctgttta cggagattat cgccgatttt tcggctgtgt
     2101 tctgcacaaa acttatcaat acattggttc accacccggg atacgtagac ttttttttca
     2161 tcatttttcc aatctgtaat attttcaccg atttgttctg aaacagagct caaaatacta
     2221 gtatttattt tggttgatga tatttttact atccttttcc ccattgtaca gcggataatc
     2281 tcatccacct ttcctgaaaa atatcttttg tgagataaac tgattccaaa attattaaaa
     2341 gatgttccaa gcattataat cactttgtca tgtaaaatag taacattgtc attattttag
     2401 ctgattcaaa caattctgta aatgctttga gttctttact cccttggctc aatgatggac
     2461 cgtctggaat cccagagact aaactctctg agaaagagag tatgaaaaaa cttggggcag
     2521 tccctaaatg aagggaccaa cagcggtaaa ataaccggca tcattcataa tgatgccctg
     2581 ttagttgtag ttatgaagat ttacgtcatt aaaattgctg ttcgtagtgt cagtcctatg
     2641 gtctggcatc agctgaggat cgtagccgat acatcactgg cttcacgaca ttcgtgttga
     2701 ggctattcat gaaaactcca cgctgaaaac gccgttttgt ataaacggtc acggtatgcc
     2761 cggagccact gttgctgatg aattcgataa aaccttggcg tttcttgaag ctatcgttaa
     2821 tgcggataat gaaacaacga ttggtgagat tcgctctttt gccgacgccc tggacgctgt
     2881 ccggttcaat cgcaacaaaa tcaaccggca gctgagcaaa cccaaccttg cctctctggc
     2941 gctggagcat gaagtgattt ggctgggacg tagccgctga cagcttctat aaaagtatga
     3001 tcctacccac gtaatatgaa cacaggctta agtgaggttc tggttttcaa attgttcagg
     3061 actgagacct ccacaccaac tgtgccgtcg tcagcgattg taatcgcact tgatataatt
     3121 aaacactgta tatttcattt gtggagtctc cagtctgagg gggctttatg cattccgtat
     3181 tgggcactat gatgccagaa atctgcaagg ctccacacct tcctgtattt cccttcctct
     3241 atcacagtgc actttactta atagggtggg atgtgttcat tacatttatt gtgtttttta
     3301 tgattgtttt tgcgtccagt gtcgttcgtc atgggaaact ggttctatgg gtagtatcgc
     3361 tcagtctagt taactgtttg tttcttttta gaaattaagc agatcacaca attcggggag
     3421 agatccttct aagtgcaatt ctttgcagct atttagcaca cttaaaacaa ctcttgtatt
     3481 ttgtcagaaa taatattaaa catgtcttgt tcattattgt taagtctgtc tgctatttct
     3541 gtaagtttta tcccactatg atggcatata tttatatatg ccatcatagc ctcgatattc
     3601 cctttccctg cggccagaaa taatcctggt aacccctcat tatctcttac atccaatagt
     3661 ttaatcgttt cttttggtga taaatcaaaa gttttaataa aatctccata agcttgaatc
     3721 gcagcggaat gaccttcttg aagagctatg aacaaagcag attccccagc attatttcgt
     3781 gctgcaagaa cttctagttt ttcctctttg ggtatttcgg ctgccaacag cgattcaccg
     3841 tatttacgcg ctgcatcggc atttccatgc caaatcaatg aaaacaaatt atggcggtta
     3901 tactccttat tattctttgt cggaatggat atgctctgta ttttggccaa ttttctgaaa
     3961 gaaacaccac tatatccccc ccctgatttt tcttcattga tggcgtggga tatagcggta
     4021 tttgtcacgt taatgatatt atttttcatt cctttatttt cattagcagt attgctgcct
     4081 attattaggt gcatattttt gttgtttgct ggatgtaatc catagttatt tattgacatt
     4141 tttgttcatc ctttttgcta aaaaaaacaa taacctattt ttgtaatgta atagatatta
     4201 gtctattgct aatctaaaaa aaccattgcc acattgaatg gctatattca ccaataaatt
     4261 ctttcgattt cgtatattga catatcgtag atatatctta tccctaagat tcaataatcg
     4321 agtccgttta ccattctgtg caagcgcctt cactcagaag tgaccgtcaa ggcgatcacc
     4381 aaacttgata ataaagcagc tcattgccat acgccagtcc ttaaggggcg ttgtccattt
     4441 ctgtgatgct gcctagattt aactgccagc tccggcttat ctacatctaa atatgtgagc
     4501 ctaataccgc tacactttgt ccagactatg tttttctccg ggaagtgggc taaccgtctc
     4561 cataaagtga cgaacttttt tcaacagtgg ccatattgaa cggcactgat gattacgggt
     4621 tattgtctca tgaagagccg gccacatcac tgacagcagt gacttcaggc cttcagcacc
     4681 agacagcctg aaccagttaa tccatcgccc gacagattta gtaatctagg gctggttttt
     4741 cttcagctgt tgcggttcga aagtgccatt acgatcacgc agagtacgga tttccagagg
     4801 ttcatctcca gtagtgataa tcttcgttaa gcaaccattg cgggaattgc tgccagatct
     4861 aagctgattt ttctcgcacc caagatgatg agtcatatta gcattgagta cggtttcatt
     4921 ttcagcatcc aatcaaactg actgagatct tcaggatttt tgagactttt ggctagttca
     4981 ttagctaaag cccgcaactt attagatgtc aactgttttt cattcataaa ttcaccttca
     5041 tttgatatca aattaaatag ttcaacatca gagaattata caaacttatg tacaggctcc
     5101 tatgatcttc gaatcctgaa gattatagtg aaaataagtt ttcacgttga aatcccacat
     5161 agttagtata tgataaataa tcatggatga cctgattaac ctcttggggt agtgctttta
     5221 tagaagaata aatattctac taatttaata catcacctaa aatagtattg ttttttatgg
     5281 ttggttgaag gtcgtggtga tgatgttagt ccttttgaga atattccagt gccagatctt
     5341 acttataata aaggggcttg gcagtttgga taaactattg ttcttattcc actaatacaa
     5401 actttttgta aagtatatta aatatacttc atttcatact gaaatccccg gaagtattca
     5461 atcaaacaat tacaccataa aaggaagagg gggccctaat taagttgtca agcatgttta
     5521 tgatacctgc agggtataaa ttttctggct gcaagcagac acatataaaa cgtctttatt
     5581 gatcctaccc acataatatg gacacagtcg taagtgaggc tctggttttc aaattgttcc
     5641 ggactgagac caccacacca acggtgccgt cgccagcgat tgtaatcgca ctcgatataa
     5701 ctaaacactg ttgcccgcac tatttcccga tggataatag tgttctccgt ggagacattc
     5761 cattttcagc gaatgaattt tcatctgttg ctgtatccag tcatacagtg actgcatcag
     5821 tggcgcggct ctggcttttc ttgccgccag acgctgttct gctgaacatc cccggacttc
     5881 tgcctcgatg gcatacagtt caccgatacg ctgcagggct tccgtggtga tgtcggttgg
     5941 cactcttgca tgcacatcgt ggatttttct ccggacatga gccatacatg cggcttccgt
     6001 tattctgccg gattcgtaca acgcccggta accaccgtaa gcatcggcct gaagcactcc
     6061 gctgtaaccg gccagatgat tctgtggatg gatacctttc cggtccggtg agtacgcgaa
     6121 ccagaccgcc ggaggcattt ccg
//
LOCUS       DAJTNM010000179         6069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTNM010000179 DAJTNM010000000
VERSION     DAJTNM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.179"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(127..276)
                     /locus_tag="OO172_003854"
     CDS             complement(127..276)
                     /locus_tag="OO172_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513833.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            1465..2280
                     /gene="sul2"
                     /locus_tag="OO172_003855"
     CDS             1465..2280
                     /gene="sul2"
                     /locus_tag="OO172_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5513834.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2367..2669
                     /locus_tag="OO172_003856"
     CDS             2367..2669
                     /locus_tag="OO172_003856"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513835.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2845..4338)
                     /locus_tag="OO172_003857"
     CDS             complement(2845..4338)
                     /locus_tag="OO172_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCR5513836.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(4382..4771)
                     /locus_tag="OO172_003858"
     CDS             complement(4382..4771)
                     /locus_tag="OO172_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513837.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4989..5429
                     /locus_tag="OO172_003859"
     CDS             4989..5429
                     /locus_tag="OO172_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5513838.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1447 a         1664 c         1592 g         1366 t
ORIGIN      
        1 aaaaaaaagg aatatttcgt tttcacgagt aatacccggg cattaaaaat ccactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt
      181 ctcttcagta acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaatcc agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag
      301 gttgttagcg ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat
      361 actggcttac tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga
      421 aaaaaggcag caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg
      481 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg
      541 ggacggaaat ttcctagaag ataccaggaa gacacttagc aaagaagaga taaggccacg
      601 gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca
      661 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
      721 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      781 ccgcggtttc tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg
      841 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa
      901 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg
      961 agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga
     1021 aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca
     1081 actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga
     1141 gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc
     1201 ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc
     1261 tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag
     1321 agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa
     1381 acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg
     1441 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1501 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1561 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1621 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1681 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1741 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     1801 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     1861 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     1921 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     1981 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2041 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2101 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2161 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2221 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2281 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
     2341 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
     2401 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     2461 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     2521 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     2581 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     2641 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     2701 ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca ctagcggctc
     2761 cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca gcattttgat
     2821 tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct cagccggtgg
     2881 cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct tcaggtgatc
     2941 aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca tggcgccgcc
     3001 gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt gcgcccatgt
     3061 catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga ccttgttgcc
     3121 cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg cgaacacccc
     3181 gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc ttgcaatgaa
     3241 atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct tgagctggta
     3301 gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg ggcggctgat
     3361 gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca cgccggcgtg
     3421 caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac tggtcggcag
     3481 agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac ggtaagtgat
     3541 cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg ccagatagct
     3601 gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca cccggtgggc
     3661 gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc agcggaaacg
     3721 cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt ggaagtgaac
     3781 attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg tcttggccac
     3841 ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt aaacgatgcc
     3901 cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct gaaacgggaa
     3961 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     4021 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     4081 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     4141 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     4201 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     4261 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     4321 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     4381 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
     4441 cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg ttattgcgtg
     4501 caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa ttaatattct
     4561 tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat gcagtataag
     4621 atgaaccact actattatca tggccaccat taccaccacc gttaaaacta cctcccgaac
     4681 ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac
     4741 cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact tttaaaaaca
     4801 gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact acgcccgtca
     4861 aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca gcctcagacc
     4921 ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca ggagacactg
     4981 tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga aagtcgcgct
     5041 cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc
     5101 gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc acttctgaga
     5161 caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg gaaaatgctc
     5221 agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc ctaatgcttg
     5281 aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac
     5341 aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg ggattctggt
     5401 cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt accataacat
     5461 ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac cggtaaaaca
     5521 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5581 gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg taagtcctgt
     5641 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5701 aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta
     5761 caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa aagtgatgta
     5821 taagtgatgt aaaagtgatg tataagtgat gtaaaagtga tgtatgaact gaaggagcga
     5881 tgtaattctg tagtcaggaa gaataacccg ttcatgcagg catgaacgat acagtgagga
     5941 acgctttttt tgtcacgatc tccccttaac ctgcgtaaat cacccgccat ggttgattat
     6001 ttttcaacct caaaatggtc atacacctaa cgcagccgcg attttcatcg caactgcatg
     6061 aattaaaag
//
LOCUS       DAJTNM010000180         6049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTNM010000180 DAJTNM010000000
VERSION     DAJTNM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.180"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            172..819
                     /gene="fucA"
                     /locus_tag="OO172_003860"
     CDS             172..819
                     /gene="fucA"
                     /locus_tag="OO172_003860"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5513839.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            847..1995
                     /gene="fucO"
                     /locus_tag="OO172_003861"
     CDS             847..1995
                     /gene="fucO"
                     /locus_tag="OO172_003861"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5513840.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2048..2803)
                     /gene="xni"
                     /locus_tag="OO172_003862"
     CDS             complement(2048..2803)
                     /gene="xni"
                     /locus_tag="OO172_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5513841.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTLAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2914..4281)
                     /gene="sdaB"
                     /locus_tag="OO172_003863"
     CDS             complement(2914..4281)
                     /gene="sdaB"
                     /locus_tag="OO172_003863"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5513842.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4339..5628)
                     /gene="sdaC"
                     /locus_tag="OO172_003864"
     CDS             complement(4339..5628)
                     /gene="sdaC"
                     /locus_tag="OO172_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5513843.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1524 a         1488 c         1615 g         1422 t
ORIGIN      
        1 aagagaactt gcagttattt actgtgatta gttgagctag gtcacaaaat ataagcttgt
       61 gaatttcttt cttgttaatc aggaagaaga tggtgggtaa ttaaacggct aattcaatag
      121 tgtgaaagga acaacattat tgccctgttt tgaatcagag agaggtagct aatggaacga
      181 aataaacttg ctcgtcagat tattgacact tgcctggaaa tgacccgcct gggactgaac
      241 caggggacag cggggaacgt cagtgtacgt tatcaggatg ggatgctgat tacgcctaca
      301 ggcattccat atgaaaaact gacggagtcg catattgtct ttattgatgg caacggtaaa
      361 catgaggaag gaaagctccc ctcaagcgaa tggcgtttcc atatggcagc ctatcaaagc
      421 agaccggatg ccaacgcggt tgttcacaat catgccgttc attgcacggc agtttccatt
      481 cttaaccgat cgatccccgc tattcactac atgattgcgg cggctggcgg taattctatt
      541 ccttgcgcgc cttatgcgac ctttggaaca cgcgaacttt ctgaacatgt tgcgctggct
      601 ctcaaaaatc gtaaggcaac tttgttacaa catcatgggc ttatcgcttg tgaggtgaat
      661 ctggaaaaag cgttatggct ggcgcatgaa gttgaagtgc tggcgcaact ttacctgacg
      721 accctggcga ttacggaccc ggtgccagtg ctgagcgatg aagagattgc cgtagtgctg
      781 gagaaattca aaacctatgg gttacggatt gaagagtaat ttcgtaaagc aacaaggaga
      841 aggatgatgg ctaacagaat gattctgaac gaaacggcat ggtttggtcg gggtgctgtt
      901 ggggctttaa ccgatgaggt gaaacgccgt ggttatcaga aggcgctgat cgtcaccgat
      961 aaaacgctgg tgcaatgcgg cgtggtggcg aaagtgaccg ataagatgga tgctgcaggg
     1021 ctggcatggg cgatttacga cggcgtagtg cccaacccaa caattactgt cgtcaaagaa
     1081 gggctcggtg tattccagaa tagcggcgcg gattacctga tcgctattgg tggtggttct
     1141 ccacaggata cttgtaaagc aattggcatt atcagcaaca acccggagtt tgccgatgtg
     1201 cgtagcctgg aagggctttc cccgaccaat aaacccagtg taccgattct ggcaatcccc
     1261 accacagcag gtactgcggc agaagtgacc attaactacg tgatcactga cgaagaaaaa
     1321 cggcgcaagt ttgtttgcgt tgatccgcat gatatcccgc aggtggcgtt tattgatgct
     1381 gacatgatgg atggtatgcc tccggcgctg aaagccgcga cgggtgtcga tgcgctcact
     1441 catgctattg aggggtatat tacccgtggc gcgtgggcac taaccgatgc actgcacatt
     1501 aaagcgattg aaatcattgc tggggcgctg cgaggatcgg ttgctggtga taaggatgcc
     1561 ggagaagaaa tggcgctcgg gcagtatgtt gcgggtatgg gcttctcgaa tgttgggtta
     1621 gggttggtgc atggtatggc gcatccactg ggcgcgtttt ataacactcc acacggtgtt
     1681 gcgaacgcca tcctgttacc gcatgtcatg cgctataacg ctgactttac cggtgagaag
     1741 taccgcgata tcgcgcgcgt tatgggcgtg aaagtggaag gtatgagcct ggaaggggcg
     1801 cgtaatgccg ctgttgaagc ggtgtttgcc ctcaaccgtg atgtcggtat tccgccacat
     1861 ttgcgtgatg ttggtgtacg caaggaagac atttcggcac tggctcaggc ggcactggat
     1921 gatgtttgta ccggtggcaa cccgcgtgaa gcaacgcttg aggatattgt agagctttac
     1981 cataccgcct ggtaaatgcg ctgatgtgat aatgccggat acgatttgcg gcgtatccgg
     2041 ctcgccgtta ccgtaccaac cgcaattgct gtaaattgcc gtcgatatgc aaatcggttt
     2101 gtaagcgggc aatatcgcgg cacagaaacg ccatctcttt atgggtttct aattttttgc
     2161 gccacttttc ggcaaccgca tccagattct catatatccc ttccagactc tgaaactcga
     2221 ccagcagctg cgtggcgctt tttggtccga ttcccgcaac acccggtacc tttgaactgc
     2281 tgatccccgc aagtccccag taatcgggca actgctgcgg ttgaacgcca aattctttat
     2341 cgataaatgg cgcatccagc caacgtttct ggaagtaatc acgaatacgt aatgtcggtg
     2401 aaagtaactg acagtagcct ttatctgtcg aaacaatcgt tgcctgatgc ccggccagtg
     2461 tcactttgac cgccagtgtg gcggctaagt catcggcttc gttgccgctg gctgaccagc
     2521 acgggacgcc gcgttgctca aaggcggcgc gtaatgcagg catctcgtcg tgcaactctt
     2581 ccggcattgg cggtcgatcc gctttgtaat ctggtaaacg ctgatgacgc cagccgctac
     2641 tgcggttttc atcatcaaaa acggcgaccg cgtgggttgg ctggctgtgc ataatgagct
     2701 gatcgagcgc atgctggcag gtttcgacac agggcgaacc ctgaacggca tgaatgcgac
     2761 ggataagatt cagtgcatcg acaataagca aatgaacagc cacgataacc cccttaaaga
     2821 tcaggcgata agggtaacat tcgatgccgg aacaggctat ggctggttga gaaaacagga
     2881 agaggcttcg caaaacgagg cctctgggag agattaatcg caggcaacga tcttcatcgc
     2941 caggccgccg cgagaggttt cgcggtactt ggcgttcatg tctttacctg tttcgtacat
     3001 ggtttcgata actttatcga ggcagacgcg cggttcgctg gtacggcgca gtgccatacg
     3061 cgcggcgttc accgctttca ctgctgcaat ggcgttacgc tcaatgcacg gtacctgaac
     3121 ctgccccgcg accgggtcac aagtcagacc gaggttgtgc tccatcgcga tttccgctgc
     3181 gatgcaaacc tgcgccgggc ttgcgcctaa cagttctgcc agaccagctg ccgccataga
     3241 gcacgccacg ccaacttcac cctggcaacc cacttcggca ccagaaatcg acgcgttcat
     3301 cttataaaga gaaccaatgg cgctggctac cagcaggtaa cgagccagtg agttagcgtt
     3361 cacttcgcgg ataaacttat cgtagtacgc cagcacagcc gggataatcc cgcacgcacc
     3421 gttagtcggc gcagtcacca cgcgaccgcc agcagcgttc tcttcgttca ctgccagtgc
     3481 aaacatgttg atccagtcaa caaccgccat cgggtcagtg gtggttttat cctggctgac
     3541 cagcatccgg cgtagtgccg cagcacggcg tggaacgcgc agtttgccag gcaacacgcc
     3601 ttcggtggaa ataccgcgct caataccgcc acgcatgact tcccagacgt tcgccaggtg
     3661 ctgttccagc tcttctttac tgtgcagcgc cagctcgttt ttcatcatca ggccagagag
     3721 tgacagcccg gtttcctgac aatgtttttg cagatcggct gctgaactgt acggataagg
     3781 aacttcaacc ggtgcgctat cctgctggcc aaaatgctct tcatcaacga taaagccacc
     3841 gccaatagag tagtaagtct ggctgtaaac gactttatcg cccgccagcg cggtaatgcg
     3901 cataccgttt tcatgcagag aaaggttgtc tgcatggaag ttcatgcact gatcaaccgg
     3961 gaactccact tcatgctgac cgtttgccag catcaggcga ccatgagtat tcacatcctg
     4021 aataaaaccg gggatggaat cgatatccac ggtatccggc aggttacccg ccaggcccat
     4081 aataatggag atatctgtgt ggtggccttt accagtcaga gagagcgagc cgtacacgtc
     4141 aaccaccacg cgggtcacgt ctttaagcag gttacgggca atcagatcgt cggtaaattg
     4201 tttacccgct ttcattggtc caacggtatg agaactggaa gggccaatgc cgattttgaa
     4261 aatatcgaat acgctaatca taggaaatac atcgcgttaa aacggaggaa gcgccgcccg
     4321 aaagcggcgc gaaaggactt agctgaacag agagtagaag attgcggaga ttgcaatcag
     4381 ccccatcacg acaacgaata cgttgctgat gtgaccgctg tacttacgca ttgccggtac
     4441 tttctgaatt gcgtacatcg gcatcaggaa caggatcatc gcgatgattg ggccgcccag
     4501 ggtttcaatc atacccagga tgctcgggtt cagggtggca acaatccagg tcgttaccag
     4561 catgaacagc gcagtgatac ggttcagctt gttgatttcg atagacttac ctttaccacg
     4621 cagagattta atcaccatac cgttgaagcc ttcacgtgcg cccaggtagt ggccgaggaa
     4681 ggatttggtg atagcgataa tcgcgataat cggagccatc cacgcgataa ccggtgcgtt
     4741 aaagtggtta gccaggtaag acagaatcga gatgttctgc tctttagccg cagccaggtc
     4801 tgccggagtc aggctcagta cgcagctgaa gacgaagaac attacggtca gcaccatcat
     4861 gatgtgtgcg aatgccagga tcttcgagca tttctgttct gccatatcgc cgtactcttc
     4921 acgcttcgca acggcgaaag aagagatgat cggagagtgg ttgaacgaga acaccattac
     4981 cggaattgcc agccacaggg tcatccacag accgtttccg gttgcagatg cagtgtccag
     5041 agacagcgtt tccagtgctg cgccgttcca ctgcgggatc aggtacagag ccagcagcat
     5101 cagtacgcca acaaacggga ataccagaat actcatcgct ttaacgatca tctgctcacc
     5161 aaagcgaacg atggtcatca tacccacgat caggatcagc gacagaatcg cacgcggcgg
     5221 tggcgtcata cccagctggt gagacatgaa gctttcaacg gtattggtga ttgccacgct
     5281 ataaaccagc aggatcgggt agatagcgaa gaagtagagc agggtaatca gtttacctgc
     5341 gccaatacca aagtgttctt ctacaacctc ggtgatgtct tcgcccgggt ttttaccaga
     5401 cagtacgaag cgagtcaggc cgcggtgagc aaaaaacgtc atcgggaacg caaggatagc
     5461 catgatgatc agcgggatca taccgccaac accggcgttg attggcagga acagcacgcc
     5521 cgcgccgatt gccgtgccgt aaaggcccag catccacatg gtgtctgtct tgcgccaggc
     5581 actacgagag tctttcgacg caatcgtgct ggtttgagtc gtttccatct atttctcctg
     5641 gaggaatgca aataattcag cttttagcca gttcagcccg ggaaggtgcc tgaccgtaaa
     5701 aatgaaatct gtctatcggc gatggcttaa tcatttaccg ctgtaataat ttgagggcgg
     5761 aaagatacac acaagttatg tatctatcag tgatcttgat cccaaataca ataatctgct
     5821 tggaagatag attatcccaa cgctttactc tgtaaatcaa aagtaaaaga gaatttaagg
     5881 gcaatatttg cccattaaac gacatctgac ttcttccggg gcgcgcaata tatgtaggtt
     5941 gaactgaaaa aatcaatact tttagttaat catggcgata atttcttttt catagcgata
     6001 aaatacggtt tatgaaaata gggaaacgtt tgcgttgcaa cataaattg
//
LOCUS       DAJTNM010000181         6026 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTNM010000181 DAJTNM010000000
VERSION     DAJTNM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.181"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..545)
                     /locus_tag="OO172_003865"
     CDS             complement(<1..545)
                     /locus_tag="OO172_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513844.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AVTDKSYLSLNPDKYPNYRFLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2193)
                     /locus_tag="OO172_003866"
     CDS             complement(1207..2193)
                     /locus_tag="OO172_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513845.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTRGTLQQDALNICDNVVRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGADRGVFMMAKRFTALSFGCFRHDGENITV
                     VLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
     gene            complement(2275..2766)
                     /locus_tag="OO172_003867"
     CDS             complement(2275..2766)
                     /locus_tag="OO172_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513846.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRVEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(3087..3815)
                     /locus_tag="OO172_003868"
     CDS             complement(3087..3815)
                     /locus_tag="OO172_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5513847.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKMRGILIA
                     FTGTDQQKQEHLLKLAQSGKVEGI"
     gene            complement(4778..5431)
                     /locus_tag="OO172_003869"
     CDS             complement(4778..5431)
                     /locus_tag="OO172_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HCR5513848.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
     gene            complement(5424..>6026)
                     /locus_tag="OO172_003870"
     CDS             complement(5424..>6026)
                     /locus_tag="OO172_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5513849.1"
                     /translation="AKAYSQKNAGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFISLVEDRRKIEGDNAM
                     VFDGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVDRLVNVMAGVYKETGVNG"
BASE COUNT         1517 a         1473 c         1481 g         1555 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgaatttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaaaacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttac tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg ggttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagccgagg acaactagct aacttcttgt ttcttaaaga ctgatgaatc cagtggttac
      841 agtgatggtt acacatggag aggctaaagg tcaagacttg aagtacgtaa cattcccaca
      901 aaaggaatag aacacagtca cggactaccc ggccctttgg tctgactgag ggtcgggcta
      961 cggggtaaat cgtcaggctt attgtattag atggtctctc acatttttca gtcaaaaact
     1021 catcgtactt taggccagac catcagcatg actatcagag ccagctttag caggaagacc
     1081 acgagataga ctttgtgtag gtccattagg tcctcctctt tccgtaccca tgaactagat
     1141 tactagtaca aaataaaggc cagacatcca gttacggaag tctgaccaat aagtcgttat
     1201 tgttagttag cttgtcttag taaagctcag gcgtttgact acaggcagga cagcagcagc
     1261 gctgccagtc cccatcttaa cagtcgttgc agcctcagcg accacgaaga ccttaccgag
     1321 aatctcaagg tcgtgagact gggtgttgcc atctcgggat aagaccacag taatattctc
     1381 gccatcgtga cggaagcaac cgaagctcag agccgtaaag cgcttagcca tcatgaagac
     1441 gccacggtct gcaccgttgt agtaggcgac gtcagtcccc agcatatcga cgatagcgtc
     1501 ttcaccaccg aagccctgct tagcagccag acgggagaga gccttgaggt gtgactcatg
     1561 ggcgataatg ccgtagtcag aaacctcaga accgaaagaa gactccgtgt tctcgtacag
     1621 ggcatccatg atagcgtcag ccttagcggt caggctacca gtcagggccg gaagctctac
     1681 ggtcttcagc agcttagagg actccagaac gtcaaccatc ttagagttaa tggtacggac
     1741 cacgttatcg cagatgttca gagcatcctg ctggagcgtc ccccgagtga aaccagtaga
     1801 ggacttctgg tcccagagct ttgtctgatg agacacgagg atagcctgtt caatcggctc
     1861 aacggtgatt tcagtaccag acggagcggt cacgacttca ggtcgcttac caccacggac
     1921 aggaacgacc acagagcggt cagccagagt gtgagcaatg attggcgtcg gtaagctgtt
     1981 acgggtcggc aggacttcta cagggaacgg aatgttttcc tgagcgtctt taagtacacc
     2041 gttcagcata gccttaaccg tcttagctaa ctggagggca tagacctgtg ggtcagcgtt
     2101 agtaggcaga ccttcattga cctgaatgtt gtcggtgtcg gtgatacgta ctggctcaat
     2161 accgaactgg aaagcgtctt taatcgtcat aataatcaat ctcctaaatt agtttaaata
     2221 ctgtgagtcc tcaaaactga ggagtcccta gcatcgggtc agggactgtg tgttttacag
     2281 gctgtccatc cagttaccgc ctgactcaag gtcagcgacg atagcggcac gggagcggcc
     2341 agcggaatgg gtagcactgc ggaggttttc accataggtc cggcgttcag ctttgacctg
     2401 ttcgcggcgc tgtagctctt tctgccattc gtcttcggtc atgaacattt cgcgggtacg
     2461 ctctttctga gacgccagct ctttagcgat acgctcagcc tcgacacgct tattcatctg
     2521 gcggcgttgg taggcggtca tcaggtcatt actgaagaag ttaccaatgg tcacgccagc
     2581 ttcagccaga gcttccagta actccattgc ctcgcggctg gtagacggca ggcctgacag
     2641 gtagcccgga atgagggtct tgatagcgag cgacagcgga tagggctgac cgtccagact
     2701 cacagtattc tcgtagagat tgatgtcggt gactgacagt ttccagtctt ggttattgaa
     2761 taacatagta attactcctc ttgagagttg agactatcgg tcgaccacag gcgtgttcag
     2821 tcctgcggcg tctccccgca tcggtcatct caaaaggttt aaaattaggt attgagtaga
     2881 cgcgctacta acggtgtgct gacgacagct agcaagctag cgacagtttc atagcgacca
     2941 gttgggctat tagctcgtct cgggattggt gctggcgggt ctcatcttcc gggtctagac
     3001 caagctctct gaggaggact tcagtccggt agccacggta acggagagtc atgcgcccct
     3061 caggtgtgcg cgggtcaatc atcatcttag attccctcca ccttaccgga ttgtgccagc
     3121 ttcaggaggt gctcttgctt ctgctggtcg gtgccagtga atgcaataag gatgcctcgc
     3181 atcttgtcaa ccatctcagg cttaccgaag acctccagcc ccttagcgtt gaaccggaag
     3241 ttatacccac ctgctgagaa cttctggtct gacggcttaa tgatacgaaa gcagtcggaa
     3301 tacttcggat ttacagacca ctcgttactg attgggttct tacgaaagag gccacgatgc
     3361 tggagaagtg cggtcattag cttctgagcg ggcagaccat aggttttgca gtagtcgccc
     3421 caagtcacgc ctcggcggtt agcggactga atggctgctc gcttcttctg gtcttccagt
     3481 tgaaggactc gacgctctaa cgtctgaata tggtcgtaac acttggcgcg gaggtgaacg
     3541 gaatagccag cgaccagaat gaaagtatgc ttacggtcga gacgatacag cggctgctgt
     3601 ttcccctgcg cgtttagata gacatcttca gtcaccccga tatgactgct cagttttgag
     3661 cactgctcag ttttgagcgc gttaagctcc tcagccatat tgcgaatatc acgcaggaca
     3721 tttcggtgct ccttgcctgt cagctcagca atttcaactg aggtcatgga gacaagctgg
     3781 tcgaggttaa tgacgctgat ttcttgttgg gacatttacg tgtactcctt ttgtttgcac
     3841 cctgaagtcg caccctactg acctactcaa gcccccacgt aaagggagac cagcaggcaa
     3901 caggacgcaa acagaatgac ttgaggtaat tgtgatgtgg tctgcgatga ccagtagtga
     3961 taaaggtgca agacagcatg tccgacgtcc agatggtgag cggcgataga cccaagtcaa
     4021 aatgaagaca cgagaacgct aacgggctgg gagtctgaca gaggctgtct tgctagcctt
     4081 ggtatggaga aagacttaat gtcggggggc ttccgcataa cttgacgcat caagaccaga
     4141 ggcggaatgt atggccccta tagtgatagc taagggtcca ttccctatag tgagggtata
     4201 agatttgcta cctgataagg ccataatctg acctccagtt agacccgaag tatggagacc
     4261 acatcatagc gaccatagtt tacttcggga tgacaggagg ctggacttaa agttcttctt
     4321 attggaattg tgataaacac aatccataac aagataaact catggtctcc tccagcctgc
     4381 tggtcaggac tcaaggttca acctcagaca ccactggtct aacctccttg agtttccgcc
     4441 tgtccacaat ggatggcaga cctcagcaat gatgtcttgg ttcttcacaa tctcgcacac
     4501 cgatggtctc atagacggaa gttccgctga ggtctgatgt tctccgcctg tcaaaggaga
     4561 gagattgcta cgttaaaggg aatggtctca ggccaaggtg atttaacact aggcgtctgc
     4621 ggatggtttc caatcggtcc gctaccggaa gcctgagact taatgaatgt gtgtgggtcg
     4681 tgaatgttat taaggtgggg acttagggtc tctcccctat agtgatagtt aagccccctt
     4741 ccctatagtg agggtatatt caatctgacc taaagcttta ctcgctagct gcctgctgct
     4801 gttccctcgc cttcctgtcc cggtcctcaa tgcccttgag cgccctttcc ggcatgctgt
     4861 taaacatggc ttcgacttct ttcggtctgg taatctgctc agtgacaata atgttcagca
     4921 gcttaaaaag accatgcgct aactgagggt cgtcatcaat attaatttca cccggatgga
     4981 ccgcctgatt gccaacgata cgacaaatat cagcagctcg ctggaccaat ggagacaatc
     5041 ctttggctac aagcgactta atgtccgtgt cgatatgctt ccccggctca ccgagatgga
     5101 ccatcagctt ctgaagactt aagcgcagga gtgcggcagc gcctttagga gaaaggttaa
     5161 caatgcttct ggcctccatg tagtcggact tacagtcgtc tggcatgtct gggttaggca
     5221 tctcaacgtt tgctgacgct ggtataatca tggtttcgtc ataccaataa gctttctcat
     5281 tgcatcgttc acatttagaa aaggcgaaag gtgtctcata aatcccgtta gcgccatatt
     5341 ttaagcggac ccagcgctgt tgagaataag cgccacaatt cgggcagtta aaagcagtgc
     5401 caccaaactc tgccggataa tactcagcca tttactccag tctccttata aacacccgcc
     5461 atcacattaa ccaatctatc tacctcgacc tctttcgcgt aacggtcgta agttatagac
     5521 ccagagctat gacccataat cgcctgagca tacgccaatt cgaccccatg ttgttttagc
     5581 tgggtcgcaa ctgtatgtct aaacgagtga aaagctaagc cgctacggtc agcaaggact
     5641 ttaggtagca acgttcggtt aaaccatttg ctcacctgct caccgtagcc gttcttcatc
     5701 agcttgaggc catcgaatac catagcatta tcaccctcaa tctttctacg gtcttctacg
     5761 agtgatataa agtcagccaa tacaaaacca taagcaccat cgaccagcgg gacacagcgg
     5821 tcactatggg cgttcttgat actcttaccc ggcaggctgc tatcgtcttc gttgatatgg
     5881 ataaacacgg tcccggcctc agtggtcctg acatctttga cctgaagttg ggccacctcg
     5941 ttaagtcttg ctccagtgat tgcagccaga gcggtgacgt aataatggta tggcttacct
     6001 gcgttcttct gagagtacgc tttagc
//
LOCUS       DAJTNM010000182         5979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTNM010000182 DAJTNM010000000
VERSION     DAJTNM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.182"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..70)
                     /gene="yjbD"
                     /locus_tag="OO172_003871"
     CDS             complement(<1..70)
                     /gene="yjbD"
                     /locus_tag="OO172_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5513850.1"
                     /translation="MALPRITQKEMTEREQRELKTLL"
     gene            complement(323..1672)
                     /gene="lysC"
                     /locus_tag="OO172_003872"
     CDS             complement(323..1672)
                     /gene="lysC"
                     /locus_tag="OO172_003872"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5513851.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2197..3846
                     /gene="pgi"
                     /locus_tag="OO172_003873"
     CDS             2197..3846
                     /gene="pgi"
                     /locus_tag="OO172_003873"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5513852.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4308..4550
                     /locus_tag="OO172_003874"
     CDS             4308..4550
                     /locus_tag="OO172_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513853.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            4664..5302
                     /locus_tag="OO172_003875"
     CDS             4664..5302
                     /locus_tag="OO172_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5513854.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5299..>5979
                     /locus_tag="OO172_003876"
     CDS             5299..>5979
                     /locus_tag="OO172_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5513855.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSET"
BASE COUNT         1496 a         1555 c         1537 g         1391 t
ORIGIN      
        1 ccagcagtgt tttaagttca cgctgctcgc gttcagtcat ctctttttgg gtaatacggg
       61 ggagtgccat gatggtgccc tcagtgagcc agagagttca gtgtacggga attcgtttgc
      121 gagcagaact gataatattc attttgcctt ctcttaacta tttatctctt tcgataatta
      181 attagagatg cagataaaaa aattaaaggc aattattctc cgataaagac gttggttaac
      241 agagtacagg ctcgtttatt gatgagcata gtgacaagaa aatcaatacg gcccgaaata
      301 tagcttccag gccatacagt atttactcaa acaaattact atgcagtttt tgcaccacct
      361 gctcggcatc ttcgccgggc accaggaagc acaggttatg gctggatgcg ccgtaacaaa
      421 tcatgcgaat gttgaacggt tccagtacgc cgaatacctc tttgccaacg ccgcaggctt
      481 ttgacaggtc attgccaatc aacgcgacca gcgccagacc ttcttccacc tccacccggc
      541 acagtgcgga aagctccatc agcagagatt gcgtcagcaa cgtatcgcca gtggaggttg
      601 aaccggtggt atcaagggtt aatgccacgc tcacttctga cgtggtgatt aagtctaccg
      661 aaatattatg ccgcgcgagg atgccgaaaa cttccgcgag gaagccgcga gaatgcagca
      721 tattcaggct gtgcaaagtg agcagagtct gattgcgacg aagcgccagc gcgcggaaca
      781 gcggcggatt ttcagtttta ttgcacacca gcgtaccacc tgcgcgtggg tctttgctgg
      841 agccgacaaa gaccgggata tcgctgcgta ctgcgggtag caacgttgcc ggatgcagta
      901 cttttgcacc aaaagttgcc atctctgccg cttctgcaaa cgcgatttca tcaatgcgtt
      961 ttgctgcgga aactacgcgt ggatcggtgg tgtagatgcc cgggacgtcg gtccagatat
     1021 caacacgaga tgcgtgtaaa gcctccgcca gcaaggctgc cgtataatcg ctgcctccac
     1081 ggccaagcgt cgttgtacga cctttatttt cgctaccgat aaatccctgg gtgatcacta
     1141 agccttcatt gaggcgtggg agcagctgca gcgcggccag ttccgccagc gcggctacat
     1201 ctggctctgc acgaccaaat cggtcgttgg tacgcatcac tttacgtaca tcaaaccact
     1261 gtgcctgaac atcgcgttcg cgcaggattt cgacaaacag cagggtcgac atcagctcgc
     1321 cgtggctgac cagctcatct gtcagcgccg gagacgttgc cagcgccgcc gcttctgcca
     1381 gaacagtaat gttctccagc agacgttcaa tctcttcacg gataacgttc gggtaacgca
     1441 gacgttccag aatggcaaac tggatgttgc gaatagcgtc gagtttttcg aatcgctcgc
     1501 caggttccag cccttccgct aaagcgacca gcagattagt gataccagca gaagccgaga
     1561 ggactactaa acgcacgttg gcatcagaaa gcacaatatc agcgctgcgg ttcatggcgt
     1621 caaaatcagc tacgctggta ccgccaaatt tggagacaac aatttcagac ataactacct
     1681 catgtcaggg gatccatttt cagccttggc acaagggaag agcggaagac gggtgggcgc
     1741 agagcgatac ttcgctacta ttttcaccca gaagtgctcc accacttgcg aaacgcccga
     1801 ctgcgaacgc ttctggtgac aacccagggg attcagcccc tgtagccgat gatgaacgtg
     1861 gccagccgtt caatcacctc ggcgatgcac cccctcaggt gttatcacag gactggctcc
     1921 tccaacaccg ttacttgggc aacgcgcctc ttctggcctg cgctagcgca ggtagtacat
     1981 ttataaataa agggtgagcg gggcggttgt caacgacggg gtcatgcgga tttttcatcc
     2041 actcctggcg gtcagtagtt cagctaataa atgcttcact gcgctaaggg tttacactca
     2101 acattacact aacggcacta aaaccatcac atttttctgt gactggcgct acaatcttcc
     2161 aaagtcacaa ttctcaaaat cagaagagta ttgctaatga aaaacatcaa tccaacgcag
     2221 accgctgcct ggcaggcact acagaaacac ttcgatgaaa tgaaagacgt tacgatcgcc
     2281 gatctttttg ctaaagacgg cgatcgtttt tctaagttct ccgcaacctt cgacgatcag
     2341 atgctggtgg attactccaa aaaccgcatc actgaagaga cgctggcgaa attacaggat
     2401 ctggcgaaag agtgcgatct ggcgggcgcg attaagtcga tgttctctgg cgagaagatc
     2461 aaccgcactg aaaaccgcgc cgtgctgcac gtagcgctgc gtaatcgtag caataccccg
     2521 attctggttg atggcaaaga tgttatgcca gaagtcaacg cggtgctgga gaagatgaaa
     2581 accttctcgg aagcgattat ctccggtgag tggaagggtt ataccggcaa agcaatcact
     2641 gacgtagtga acatcgggat cggcggttct gacctcggcc cgtacatggt gaccgaagca
     2701 ctgcgtccgt acaaaaacca cctgaacatg cactttgttt ctaacgtcga tgggactcac
     2761 atcgcggaag tgctgaaaaa agtaaacccg gaaaccacgc tgttcttggt agcatctaaa
     2821 accttcacca ctcaggaaac catgaccaac gcccatagcg cgcgtgactg gttcctgaaa
     2881 gcggcaggtg atgaaaaaca cgttgcgaaa cattttgcgg cgctctcaac caatgccaaa
     2941 gccgttggcg agtttggtat tgatactgcc aacatgttcg agttctggga ctgggtcggc
     3001 ggccgttact ctttgtggtc agcgattggc ctgtcgattg ttctctccat cggctttgat
     3061 aacttcgttg aactgctttc tggcgcacac gcaatggaca agcatttctc caccacgcct
     3121 gccgagaaaa acctgcctgt cctgctggcg ctgattggca tctggtacaa caatttcttt
     3181 ggtgcggaaa ctgaagcgat tctgccgtat gaccagtata tgcaccgttt cgcggcgtac
     3241 ttccagcagg gcaatatgga gtccaacggt aagtatgttg accgtaacgg taacgttgtg
     3301 gattaccaga ctggcccgat tatctggggt gaaccaggca ctaacggtca gcacgcgttc
     3361 taccagctga tccaccaggg aaccaaaatg gtaccgtgcg atttcatcgc cccggctatc
     3421 acccataacc cgctctctga tcaccaccag aaactgctgt ctaacttctt cgcccagacc
     3481 gaagcgctgg cgtttggtaa atcccgcgaa gtggttgagc aggaatatcg cgatcagggt
     3541 aaagatccgg caacgcttga ctacgtggtg ccgttcaaag tgttcgaagg taaccgcccg
     3601 accaactcca tcctgctgcg cgaaatcact ccgttcagcc tgggtgcgtt gattgcgctg
     3661 tatgagcaca aaatctttac tcagggcgtg atcctgaaca tcttcacctt cgaccagtgg
     3721 ggcgtggagc tgggtaaaca actggcaaac cgtattctgc cagagctgaa agatgataaa
     3781 gaaatcagca gccacgatag ctcgaccaat ggtctgatta accgctataa agcgtggcgc
     3841 ggttaatcat catgcgtgcc tgatgccgat atgtaggccg gataaggcgt ttacgccgca
     3901 tccggcaacc ggagcctgat gccaatatcg acattaatgc atagtatgag gagttaaata
     3961 accttaaaaa caatgccgac aataagtcgg cattgtttta tcagataaat ccccttgtct
     4021 gtgatttaac ggaaatcata ccgtgaggtt aatcctaaaa tagattttta atcgttgttt
     4081 atttcggaga atacgcagat taattacttt tgtttttatt ttaagtttat gatttttatt
     4141 gttatttaaa tataagttga aacttatatt tgatattcat tccaattatc ctaaaactcc
     4201 atcgctaatt ccccgcgccg taattcgcat gctttagttg tgtatactcg atcccgcccg
     4261 aaatgttttt gggtaaatct ccattcattc aatgaaggga aattgttatg aaaaaagttc
     4321 tgtatggcat ttttgccata tctgcgcttg cggcgacttc tgcgtgggct gcacctgtac
     4381 aggtgggcga agcggcagag tcggcagcaa cgtcggtttc ggcggggagt tcctccgcga
     4441 ccagcgtcag caccgtaagc tcggcggtgg gtgtcgcgct cgcggtaacc ggtggcggtg
     4501 atggttctaa taccgggacc accacaacca cgaccaccag tacccagtaa taaagtatgt
     4561 atccccaaaa taatttgagt cattgcatct gtggcttgaa gtatgaaggg atttaccata
     4621 accacactcc ggtgtggtta ttctgcccct ctggagaaga gttgtgaagc gacctgcact
     4681 cattcttatc tgcctgctat tacaggcctg ttcagccacg actaaagagc tgggcaattc
     4741 actgtgggac agtctgttcg gcacgccagg cgtacagctg acggacgatg atattcaaaa
     4801 tatgccctac gccagccagt acatgcagct taatggcggg ccgcaattgt ttgtggtgct
     4861 ggccttcgct gaagacggac aacaaaaatg ggtcactcag gatcaagcca ctcttgtcac
     4921 acaacatggt cgtctggtga agactttgct tggcggcgac aacctgattg aagtgaataa
     4981 ccttgccgcc gacccgctga ttaaacccgc acaaattgtt gatggtgcaa catggacgcg
     5041 cacgatgggc tggaccgagt accagcaggt acgctacgcc accgcacgct cagtcttcaa
     5101 atgggatggc accgataccg tcaaggtcgg cagcgatgaa accctggttc gcgtgctgga
     5161 cgaagaagtc tccaccgacc aggcgcgctg gcataaccgc tactggatcg acagcgaagg
     5221 gcaaattcgc cagtcggaac agtatctcgg cgcggattat ttcccggtga aaaccactct
     5281 catcaaggcg gcaaaacaat gattaaacaa actattgtcg cgttgctttt gagtgtggga
     5341 gcgtcatcgg tcttcgcggc aggaaccgtc aaggtgttca gcaatggcag cagtgaggcc
     5401 aaaacgctaa cgggcgcaga gcatttaatc gatctggtag ggcaacctcg actggcaaac
     5461 agctggtggc ctggtgcggt gattagcgaa gagctggcaa cggcggcagc attgcgtcag
     5521 cagcaggcgt tgctgacacg gctggcagag cagggcgcag attccagcac cgacgatgcc
     5581 gccgccatta acgccttacg ccagcaaatc caggcattga aagtgacagg caggcaaaaa
     5641 atcaatcttg atcccgatat cgtccgcgtt gccgaacgcg gtaacccgcc gttgcagggc
     5701 aactacacgc tgtgggtcgg accaccgccg tccacggtca cgttgttcgg gcttatcagc
     5761 catcctggca atcagccatt cactccaggt cgcgacgtgg cgagctatct ctctgaccag
     5821 agcctgctca gcggtgcgga tcgcagctac gcgtgggtcg tttacccgga cggacgcacg
     5881 caaaaagcgc cggtggctta ctggaacaag cgtcacgtag agccgatgcc cggcagcatt
     5941 atttatgttg gcctcgcaga ttccgtctgg agtgagact
//
LOCUS       DAJTNM010000183         5975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTNM010000183 DAJTNM010000000
VERSION     DAJTNM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.183"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            85..315
                     /locus_tag="OO172_003877"
                     /pseudo
     CDS             85..315
                     /locus_tag="OO172_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
     gene            complement(584..2089)
                     /gene="glpD"
                     /locus_tag="OO172_003878"
     CDS             complement(584..2089)
                     /gene="glpD"
                     /locus_tag="OO172_003878"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5513856.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            2279..2605
                     /gene="glpE"
                     /locus_tag="OO172_003879"
     CDS             2279..2605
                     /gene="glpE"
                     /locus_tag="OO172_003879"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5513857.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2650..3480
                     /gene="glpG"
                     /locus_tag="OO172_003880"
     CDS             2650..3480
                     /gene="glpG"
                     /locus_tag="OO172_003880"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5513858.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            3497..4255
                     /locus_tag="OO172_003881"
     CDS             3497..4255
                     /locus_tag="OO172_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5513859.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(4237..5835)
                     /gene="rtcR"
                     /locus_tag="OO172_003882"
     CDS             complement(4237..5835)
                     /gene="rtcR"
                     /locus_tag="OO172_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5513860.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPCGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         1356 a         1557 c         1594 g         1468 t
ORIGIN      
        1 ccaaagggtc ccgttttttt cgccatcatt tcggaaaagt gtccagaagg ggcaagggga
       61 gtataatttc gccattcaga aagaatggaa agaataatgc aaaatagaaa atggattttg
      121 acctcgctgg taatgacttt tttcggcatc ccaatactga cgcaattttt tgcagcggtt
      181 attgccatgc tgggggctgg gcttgccgct attcctgagg tttgcaattt actctttacg
      241 ccaacaattt accttctgct caatattttt atgctgacgc tgggcgcatt aatgctattt
      301 ttctcggggc gagtggggaa cgtgcctgag ttttgttacg ttgggtatga tggcgtgggc
      361 tttgcggtaa ttccctgaat taaaaagtag gttagatttt tgacaaagtg cgctttgttc
      421 atgccggatg cgacgtgaac gtcttatctg gcctacaaga ccatgcaaat tcaatatatt
      481 gcataaacca cgtaggcctg ataagcgtag cgcatcaggc agttttgtgt ttgtcatcag
      541 tctcaggcca gattgaaatc tgacctgatc accttacgtt aatttacgac gccagcgaca
      601 acttctgctg cgtatattcc accagccact gactcacacg agattgttga tccgcattca
      661 gccacatgcc ttgttttgtg cgacgccaca gggcatcgtc ggtgcggcgg acccattcgt
      721 gatccaccag gtatttcagt tccgcttcgt agaactcatg accgaaatct tccccgagat
      781 cgcttaccgt tcccgcattg ccgagcagca gctcgctgtt gctgccgtaa gtgcgcgcgt
      841 agtgacgtgc cagcgattca gtcaggaacg ggtaacggcg gcgcaggcga gcggcgtaat
      901 cgtcgcggtc gccttcaatg gcaccacccg gcagcacgct ctctttcgtc cacgccgggc
      961 caataccctg ataatacggc gtcagttttt ccagcgcatg ttctgccagt tttcggtagg
     1021 tggtcagctt accgccgaat accgacagca gcggtgcttt gccgttttca tcatggatat
     1081 caagggtgta atcacgggta atggcttgcg gcgagtcgga ctcatcatca cacagcggac
     1141 gcacaccgga gtaggtccag acgatatcgt cacggcttag ctgcttttta aagtgcgcgt
     1201 tatacacttt cagcaggtaa ttgatttcac tctcttcaat cttcactgct ttcggatcac
     1261 ctttgtattc cacatcggtg gtgccgatga tggaaaactc gtccatccac gggatcacga
     1321 acacaatgcg tttatcttcg ttttgcagaa tgtaggcctg cttttgggta tgcacgcgcg
     1381 gcaccacaat atggctgcct ttgatcaggc gaatgccata aggcgaaggc agatgcatcc
     1441 cctcgtcgaa gaactgtttc acccacgggc cggtggcgtt aaccaggccg cgcgcttgcc
     1501 agctatattt tttgccggta tcgatatctt ccgcttccac aatccacagg ccgttttcgc
     1561 ggcgagcaga ggtggcgcga gtccgagtaa gcacttcgcc gcctttacgc accaccatct
     1621 gggcgttggc gagtaccaga cgggcgtcgt ctacccaaca gtcagaatat tcgaatccgc
     1681 gcttaatttc cggttttaac actgaatttg cgccaaaacg caaaccaatt gatcccggca
     1741 ggctggtgcg tttacccaga tgatcgtaca taaacagacc aatgcgaatc atccacgccg
     1801 ggcgcagatg cggacgatgt ggcaggcgaa aacgcatcgg gaaggcgata tgcggagcca
     1861 ttttcagcag cacttcacgt tcagccagcg cctcgctgac caggcggaat tcatagtgct
     1921 caaggtagcg caggccaccg tgaatgagtt ttgaactggc ggaagaggtc gcgcaagcga
     1981 gatcctgcgc ctccagcatc agcacggata aaccgcgtcc agcggcgtct gccgcgatac
     2041 cagcaccatt gatgccgcca cctatcacaa tcagatcttt ggtttccatg ctgccctcat
     2101 tcactttcgt taaagctcat aaatgttcgt tatcgaacat attagcaaag aatcgcgctt
     2161 tgggtaacat tgaaaaaaca ttttagagtg atatgtataa cattatggcg tttatctgcc
     2221 gcttcgacgt aaactgtgcg gtaaatttgc ccacttgttt gtaaagaaag agagacgcat
     2281 ggatcagttc gaatgtatta acgttgccga cgcgcaccag aagttgcagg aaaaagaggc
     2341 ggtgctggtc gatattcgcg atccacagag tttcgctatg gggcatgcgg tgcaggcttt
     2401 ccatttaacc aacgacacgc tgggcgcgtt tatgcgtgat aacgactttg acactccggt
     2461 gatggtgatg tgttatcacg gcaatagcag caaaggggcg gcgcagtatc tgctgcaaca
     2521 gggctacgat gtggtctata gcattgacgg cggctttgaa gcctggcagc gacagtttcc
     2581 cgcagaggtg gcgtacggcg cgtaacgctt tatactgtcc ccttttgtgt ggaataagcg
     2641 acagcaacga tgttgatgat tacctctttt gctaaccccc gcgtggcgca ggcgtttgtt
     2701 gattacatgg cgacgcaggg tgttatcctc acgattcaac aacataacca aagcgatgtc
     2761 tggctggcgg atgagtccca ggccgagcgc gtgcgggcgg agctggcgcg ttttctcgaa
     2821 aacccggcag atccgcgtta tctggcggcc agctggctgg caggccatac cggcagtggc
     2881 ctgcattatc gccgttatcc tttctttgct gccttgcgtg aacgcgcagg tccggtgacc
     2941 tgggtgatga tgatcgcctg cgtggtggtg tttattgcca tgcaaattct cggcgatcag
     3001 gaagtgatgt tatggctggc ctggccattc gatccaacac tgaaatttga gttctggcgt
     3061 tacttcaccc acgcgttaat gcacttctcg ctgatgcata tcctctttaa cctgctttgg
     3121 tggtggtatc tcggcggcgc ggtggaaaaa cgcctcggta gcggtaagct aattgtcatt
     3181 acgcttatca gcgccctgtt aagcggctat gtgcagcaaa aattcagcgg gccgtggttt
     3241 ggcgggcttt ctggcgtggt gtatgcgctg atgggctacg tctggctacg tggcgaacgc
     3301 gatccgcaaa gtggcattta cctgcaacgt gggttaatta tctttgcgct gatctggatt
     3361 gtcgccggat ggtttgattt gtttgggatg tcgatggcga acggagcaca catcgccgga
     3421 ttagccgtgg gtttagcgat ggcttttgtt gattcgctca atgcgcgaaa acgaaaataa
     3481 ttccagggat ttataaatga aacaaacaca acgtcacaac ggtattatcg aactggttaa
     3541 acagcagggt tatgtcagta ccgaggaact ggtggagcac ttctccgtca gcccgcagac
     3601 tattcgccgc gacctcaacg agctggcaga gcaaaacctg atcctgcgcc atcatggcgg
     3661 cgcggcgctg ccttccagtt cggttaacac gccgtggcac gatcgtaaag ccacccagac
     3721 cgaagaaaaa gagcgcatcg cccgcaaagt ggcggagcaa atccccaatg gctcgacgct
     3781 gtttatcgat atcggcacca cgccggaagc ggtggcgcac gcgctgctca atcacagcaa
     3841 tttgcgcatt gtcaccaaca atctcaacgt tgctaacacg ttgatggtaa aagaagattt
     3901 ccgcattatt ctcgccggtg gcgaattacg tagccgcgat ggcgggatca ttggcgaagc
     3961 gacgctcgat tttatctccc agttccgcct tgatttcggc attctgggga tcagcggcat
     4021 cgacagcgac ggctcgctgc tggagttcga ttaccacgaa gttcgtacca agcgcgccat
     4081 tattgagaac tcgcgccacg ttatgctggt ggttgaccat tcaaaatttg gccgtaatgc
     4141 gatggtcaat atggggagca tcagcatggt tgatgccgtc tacaccgacg ccccgccgcc
     4201 agtaagcgtg atgcaggtgc tgacggacca ccatattcaa ctggagctgt gctgatcctg
     4261 cacggcctcc cacgtcagac caaaacgcgc caggtatttg cgtagccgat ccgcgtcatt
     4321 gacgctggct ttgccctggc gcgaaacatc aaaaagctgt cgtccggcag cagaaagcga
     4381 ctttgcctgg cggcagatag cgataacgtg ttccagttgc atacggtcga agagatcgat
     4441 gttttctgcc tctgcgccca gcaacgccgt aagcgcggag gggcggctct cctgccagtt
     4501 atagcgcaga cggtttatct catcttccac cacgtccaga gtgatgcgtc cgctagtggc
     4561 aaaggtggcc atccgcgtga cgctggcaga aagttcgcga aagttaccgc gccatgtagc
     4621 ctggggagat gttgcaaagg ccagccaggc acgccgcgct tcggtgttaa aacgcacgct
     4681 gtcaccagtg agtgtggcgt ggcgctccac ttcataatcc aggttcggtt caatatcttc
     4741 ctggcgctgg cgtagacccg gcagggtgaa ggtccagaga ttgatccgcg cgtacagatc
     4801 ttcgcggaat ttgccttcgg caaccagctg gcgcaaatcg cgcaccgttc cggcgataag
     4861 ctgaaaatca ctgctcacct ggcgatcgct gccaaacggg taaaaggttt tctcttcaat
     4921 agctttcagc agcattgcct gttcgtctgc gcccagttcg ccaatctcat caagaaacaa
     4981 cattccgccg ttggcgctgc gtaataatcc ttcacgagat tcccgcgccc cggtaaacgc
     5041 gcctttcaca tgaccaaaca gcgtcgacat cgcggtatcg ccgcgcaggg tggcgcagtt
     5101 cacttccaca aacgcgccgc taaactgatg ccgtgcctgt tttaactcga agatgcgtcg
     5161 cgccagaaat gacttacctg cgccggtcgg gccgttaagc agaatcggcg cgcgggattt
     5221 aatcgccact ttttcgatct gctcaatcat gcggttgaag tgggggttac gcgtggcaat
     5281 accggatttg aggaaatcga gcgtttgctg ccgttcctcg gcaaagcggc tggcgatggc
     5341 gttataacgg cttaaatcga gatcgataat cgtcacttca cctgggccgc acggctgttc
     5401 ttttttgcgc ggagttgaag attgtatcaa ccgggcgggt agataacgcg cttccgccag
     5461 cagaaaccag caaatctgcg cgacgtgggt gccggtggtg atgtgaatta aatagtcttc
     5521 tttttctggc tgaaactcgt aaccacgggc gaaatcatgc aggcaggcgt aaacctcttc
     5581 gaaatcccac gggttatgca gttcaatctc aacgcccacc acttctgttt ctggcgaaac
     5641 gctggcgata tcgcgtttaa gcgtttcaaa tagcgagcgc gagcgggcat cgtgcaataa
     5701 ttccagtcga tcgatgacca acgattcttg ctggcatata cagagtgacg gacgccattt
     5761 tgaccagcgc tgactgccgc gtccggcata atccagcacg gtaccgacaa agccaaaagc
     5821 cactgtttta cgcatcttag atatccttat aaaaggcgat atttcattat cggtaaatcg
     5881 gctttgcggg aaatctccgt ttacggacat aaagatataa tctttttaga aacaataaat
     5941 taaaaaatta atctgcttat ttaattttct ggcac
//
LOCUS       DAJTNM010000184         5933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTNM010000184 DAJTNM010000000
VERSION     DAJTNM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.184"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..112)
                     /locus_tag="OO172_003883"
     CDS             complement(<1..112)
                     /locus_tag="OO172_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HCR5513861.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGT"
     gene            complement(369..650)
                     /locus_tag="OO172_003884"
     CDS             complement(369..650)
                     /locus_tag="OO172_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5513862.1"
                     /translation="MWGVELLAIRYAAWIKPEFEIEVYEVFKTVVRLGVGAMSRLNRI
                     DHIINTETKAISQCASQMAKWGVGGRKRLLHVARERAANEVQMYLPGMV"
     gene            complement(674..874)
                     /locus_tag="OO172_003885"
     CDS             complement(674..874)
                     /locus_tag="OO172_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR5513863.1"
                     /translation="MKYPTVIVNGVSVRVDEDGRYNLNDLHAAAVANGEATEQQRPSQ
                     FLRSAQIKRFIKALWNKFNHLK"
     gene            complement(1080..1232)
                     /locus_tag="OO172_003886"
     CDS             complement(1080..1232)
                     /locus_tag="OO172_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513864.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSKSRLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(1220..1687)
                     /locus_tag="OO172_003887"
     CDS             complement(1220..1687)
                     /locus_tag="OO172_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5513865.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(1684..2160)
                     /locus_tag="OO172_003888"
     CDS             complement(1684..2160)
                     /locus_tag="OO172_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5513866.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
     gene            complement(2144..2467)
                     /locus_tag="OO172_003889"
     CDS             complement(2144..2467)
                     /locus_tag="OO172_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR5513867.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            2692..2910
                     /locus_tag="OO172_003890"
     CDS             2692..2910
                     /locus_tag="OO172_003890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513868.1"
                     /translation="MATAQFGLWRPVLISGLLQLPTAGYVATPNPAKDSCPYTSACAF
                     TTTERALANQARRLFTIIGSMLLPVVQI"
     gene            complement(3179..3802)
                     /locus_tag="OO172_003891"
     CDS             complement(3179..3802)
                     /locus_tag="OO172_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5513869.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTELWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(3799..3987)
                     /locus_tag="OO172_003892"
     CDS             complement(3799..3987)
                     /locus_tag="OO172_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5513870.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(3984..4346)
                     /locus_tag="OO172_003893"
     CDS             complement(3984..4346)
                     /locus_tag="OO172_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5513871.1"
                     /translation="MSEYQFVLPYPPSVNTYWRRRGNQYYISDKGQKYRKEVQQIILQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(4343..4633)
                     /locus_tag="OO172_003894"
     CDS             complement(4343..4633)
                     /locus_tag="OO172_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5513872.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(4633..5355)
                     /locus_tag="OO172_003895"
     CDS             complement(4633..5355)
                     /locus_tag="OO172_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCR5513873.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEDCGRINGLGLNQSFCVYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(5348..5557)
                     /locus_tag="OO172_003896"
     CDS             complement(5348..5557)
                     /locus_tag="OO172_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCR5513874.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDE"
     gene            complement(5517..5918)
                     /locus_tag="OO172_003897"
     CDS             complement(5517..5918)
                     /locus_tag="OO172_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513875.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIQLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANTTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
BASE COUNT         1347 a         1522 c         1346 g         1718 t
ORIGIN      
        1 cagtgccaat gaactgaaat gcttcttctg cggctgcgtt ctggttcata accagtttgt
       61 aaatctctaa ctggaatttc tgttcttcag tcatgggaat aatctctgcc attgttggct
      121 ccgtttatcc gttaaaaggg atatcagtta agttatcccg tgtagggtat aagccattat
      181 cgagaccact cattgaatgg cctctgcaat aaccgatgtc tttccatcag tccgccacca
      241 caaagaatct tttttgccat aaggctggag gttcatcttt cagtggctgc cagtgttatt
      301 tccccactta ctggcttggg ttgtttcgcg gtactgccgt aatgcaaaaa ctggattaac
      361 ctgcgaaatc acaccattcc gggcaaatac atttgcactt catttgccgc tctctcacgt
      421 gcaacatgaa gcaatctttt tcgcccacca acgccccact tagccatttg gcttgcgcac
      481 tggcttatcg ctttggtttc agtattgatg atgtgatcga ttctattcag acgggacatt
      541 gcgccaacgc cgagacggac aaccgttttg aaaacttcat aaacttcgat ttcaaattcc
      601 ggcttaatcc atgctgcata tctgattgcc agaagttcaa caccccacac acctggttct
      661 gcaccacctt tgattatttt aagtggttga atttgttcca aagtgctttt atgaagcgtt
      721 ttatctgcgc gctacgcaaa aactggcttg ggcgctgttg ctctgtagcc tctccatttg
      781 caactgctgc tgcatggaga tcgtttaagt tgtagcgtcc atcctcatca acacgaacgg
      841 acacaccatt gacaataact gttgggtact tcatcagtga ttacctttta gtgatgaacc
      901 ttgtcacaca ggattccggc ccacagaaag gcaccgatta ccaaaccggc atcctcaagg
      961 gtcatcctga aaggctctgt gttcataagt cgcgcgtgtg aagcgcgttt gttgcagata
     1021 taaaaaagcc ccgcgaatgc gaggctaaat cctggtgttt gtgatgactg gctcttatct
     1081 caacgcagcc ccttaccgcg cgccagatgc tcaatatcaa gcatcagcaa tgagatgttt
     1141 aatctggatt tactccagaa gtgatcaccc ccctgtctac agagccagat gtgaaggatg
     1201 atgagtaaaa ttatcgctat catcgaaggc attgcgtcct gatgtattcc tgaagcgttc
     1261 tcagtgctgt ttggtcgcgg ataattccgt cccggatacc gagaacgttt cgtccagcaa
     1321 ctggagagag ttcgacggtg gcatcattgc ccatgccgga ggcgctggag gtttcggctg
     1381 aggatggcac agggcatttt cctttgacga gcaccctgcc accattatca agcttgcgcc
     1441 gaagagcatc attttcagct ttcgcatcag ctaactcctt cgtgtattta gcatcgagtg
     1501 catcagcagc acgctggcgt tgctgcatgt cagtaatggt ggcggtcgcc tgcttcagct
     1561 cactgacttt tttatctcgc tgctctttgt aggtcatggc gttatcacgg taatgattaa
     1621 ccgcccatga caggcagacg atgatgcaga taaccagagc ggagataatc gcggtgactc
     1681 tgctcataca tcaatctctc tgaccgttcc gcctgcttct ttgaattttg caatcaggct
     1741 gtcagcctta tgctcgaact gaccataacc agcgcccgga agtgaagccc agatattgct
     1801 gcaacggtca attgcctgac gaatatcacc gcgatcaatc atcggtaaag cgccacgttc
     1861 tttaatctgt tgcagtgcca cagcgtcctg gcttttcgga gagaagtctt tcaggccaag
     1921 ctgcttacgg taagcatccc accaacggga aagaagctga taacgtccgg cggctgttga
     1981 ttttagtttt gggtttagtg tgacaagttt gcgaggatga tcggagtaat cagtaaatag
     2041 ctctccgcct acaatgacgt cataaccatg atttctggtt ttctgacgtc cgttatcagt
     2101 tccctctgac cacgccagca tatcgaggaa cgccttacgt tgattattga tttccaccat
     2161 cttctactcc ggctttttta gcagcgaagc gtttgataag cgaaccaatc gagtcagtac
     2221 cgatgtagcc gatgaacacg ctcgttatat aagcgagatt gctacttagt ccggcgaagt
     2281 cgagaaggtc acgaatgaac caggcgatga tggcgcacat cgttgcgtcg attactgttt
     2341 ttgtaaacgc accgccatta tatctgccgc gaaggtacgc cattgcaaac gcaaggattg
     2401 ccccgatgcc ttgttccttt gccgcgagaa tggcggctaa caggtcatgt ttttctggca
     2461 tcttcatgtc ttacccccaa taaggggatt tgctctattt aattaggaat aaggtcgatt
     2521 actgatagaa caaatccagg ctactgtgtt tagtaatcag atttgttcgt gaccgatatg
     2581 cacgggcaaa acggcattag gttgttagcg caacctcatg ccgcccactt tcacgaagat
     2641 catgtgtaga aggccgcagc gtaactatca ctgatgaatt caggatagtc agtggctacg
     2701 gctcagtttg gattgtggcg accggtgctg atctccggtt tgctgcaact gcctacagct
     2761 ggctacgtgg ccacaccgaa tccagcgaaa gattcttgcc cttacacatc agcctgtgca
     2821 ttcaccacaa cggaaagagc actggctaac caggctcgcc gactcttcac gattatcggc
     2881 tcaatgctct tacctgttgt gcagatataa aaaatcccga aaccgttatg caggctctaa
     2941 ctattacctg cgaactgttt cgggattgca ttttgcagac ctctcagcct gcgatggttg
     3001 gagttccaga cgatacgtcg aagtgaccaa ctaggcggaa tcggtagtaa gcgccgcctc
     3061 ttttcatctc actaccacaa cgagcgaatt aacccatcgt tgggtcaaat ttacccaact
     3121 ttattcaata agtcaatatc atgccgttaa tatgttgcca tccgtggcaa tcatgctgct
     3181 aacgtgtgac cgcattcaaa atattgtctg cgattgactc ttccttgtgg cattgcacca
     3241 ccagagcgtc atacagcggc ttaacagtgc gtgaccatgt gggttgggta aggttgggga
     3301 ttagcatcgt tacagcgcga tatgcggcgc ttgctggcat ccttgaatag ccgacgcctt
     3361 tgcatcttcc acactctttc tcaacaactc tcccccacag ctctgttttg gcaatatcaa
     3421 ccgcacggcc tgtaccgtgg caatctctgc atcttgcgcc cggcgtcgcg gcactacggc
     3481 aataatccgc ataagcgaat gttgcgagca cttgcagtac ctttgcctta gtatttcctt
     3541 caagctttgc aacgccacgg tatttccccg ataccttgtg tgcaaattgc atcagatagt
     3601 tgatagcctt ttgtttgtcg ttctggctga gttcgtgctt accacagaat gcagccattc
     3661 cgaatccggc ttgtgattgc gccatcccca tagcagccat cacatcagta ccggaaagag
     3721 agtcagaagc cgtggcccgt ggtgagtcgc tcatcatcgg gctttttggc gaatgaaatt
     3781 tagctacgct ttcgagtctc atcgtcttcc cctcttgccc tgtttgacca tcaggacgcc
     3841 gttaactatt acgtgacgct cgcctttgct gtctcggttg tacttgagca ctgttcctct
     3901 tgcgcaggaa agcatcctcg ccacttcggt ctgattgcct cgtgtctgga taagaagctc
     3961 tggtatcgtt tgaattgtgg cgttcatacg ttctccagtt cggtgatttt tattccaagc
     4021 cttccgcctg gtactttcac accacgaatt acgcgaatgt catcgaattg ctcgtcgtct
     4081 tccgcaaatc cggcgtggat aagggagtcg agtaaacctt tcaggatgtt atcgaggtcg
     4141 cggcggcggg agtctggaac gtctgcgatg actttgatgc ggagtcgtga tttggtgaaa
     4201 atgtctaact tgagttggag gattatttgc tgaacttctt ttcggtattt ctggccttta
     4261 tcgctgatgt agtattggtt tccccgtctt cgccagtagg tgttcaccga cggcgggtat
     4321 ggaagcacaa actgatattc gctcatgact taatcttccc ctccttcagc agtatcgcct
     4381 gcgtcctgat cacgccttcg aggtggtaaa gtctggcgtc tttgttgtcg agattatggg
     4441 tgcgtcggtc gatttcatcg tgacacgcgc tacaagccca tgcgccgatc aggtcgtcag
     4501 gcttcattcc cgttccgcaa attccagcca tccggtaatg tgccagaact gtagtttcag
     4561 gattaccatt gcatacgccg taaatacgta cctggcattc tctgccgcgt gcttctttgc
     4621 gtagattagc cattaagcag cctcccctgt tactttcagc attccattat cgagcagctt
     4681 tctggtcagc cactgttgac cacgcccggt gatttttgtg gtgaacgata tctgtattcc
     4741 gtgatttgtg ttgaccgctg tttctttcac tgtgaaatag ccgcgatcca tatattcctg
     4801 cattggcaca ttgcgccggg aacctgaagc aataaggatt ttgtgatcgc gcatccacgc
     4861 aaacagtttg tttggaccaa ttccaacaac ctttgcaaag tttccaatca aaattccgct
     4921 ggcctcgcca acgcgatcgg caaactcaac tttaggtgcg gcaattgcga gctggttttc
     4981 cagttgcatt ttctgctcag caagatcagc agcaaggcgc aacgcttccg gtagcgtttt
     5041 ggggatatta accgcagctt cttcaagctc tcgccagcgg tcaacaaggc gagcggtgaa
     5101 ttccggcgac aactgggcta caacgacaat actgtctcgc tttccttgtt cgccttcgaa
     5161 cacatacaca caaaaacttt gatttaagcc taacccattg attcttccac aatcctcaat
     5221 ttgaggaagc cggataacac catttttagc cagcgtttcg atggtacgtt tcacattgtc
     5281 atgacgctta ccaaccaact cagcgatttc aatgcttgtc attttgatgg cattgccatt
     5341 tattaactca ttcatcgtct tcttcctcgt acattgagct attcggatcg ctcatcagtt
     5401 ctgcgcagca gtgctcacac acgtgaactt ccagcacatg cagcttctga ccgcaattag
     5461 cgcacgttaa agctcgctcg acgctttctt tctggtattg aagggattgg gatgggctaa
     5521 gcatggcttt caccattaaa aagtcgcttg taagcatcga tgtctcgttt tgcttcaccg
     5581 agctttcgtc ttaattccat gttttctgat tcaagctttt ccatgtcttg ttggtatcga
     5641 tcgcggtgtt ctttccatgc ttttcgatac gccttcatgt atgtcgtatt ggcctttctc
     5701 tttgcctgac gaactgcgtg gtggtttttc acaaaccagt cagggtcgtt aaatgctgct
     5761 ctggcgcagg tataccaata atttgttgcc tccctgttta gccaataaat actgataaat
     5821 ggcaactgta tcgacaccat ttttcgttga gactctttct cgccaaacat gtgccctttt
     5881 ttgatgctaa ggccaaatcc aggttgaatt aaaagcattg tcatttcctc gca
//
LOCUS       DAJTNM010000185         5893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTNM010000185 DAJTNM010000000
VERSION     DAJTNM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.185"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..422
                     /locus_tag="OO172_003898"
     CDS             <1..422
                     /locus_tag="OO172_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5513876.1"
                     /translation="LQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDINDNL
                     LFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKKTVAAIAPSLSTSTQTR
                     VSASSCKVEFRHGNMTLENPSPELLTVLIRELSGRGR"
     gene            419..709
                     /gene="tnpB"
                     /locus_tag="OO172_003899"
     CDS             419..709
                     /gene="tnpB"
                     /locus_tag="OO172_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5513877.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAML"
     gene            complement(961..1704)
                     /locus_tag="OO172_003900"
     CDS             complement(961..1704)
                     /locus_tag="OO172_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR5513878.1"
                     /translation="MVKILAVAQSHESIVSMDEINSILLSSSWFRALPDYLIEQMLSR
                     TSLYQYNDGEIIHYRGAEPLGLYAVITGSVKVSSISADGKECIFRYLSPGNWFGEIGM
                     IDKCPRTHDARATGKTTLLTIKPHDVEFLLKNYPILHRFLALLLCKVVRNAFTMLHDC
                     TLLSVSARLAKRLVSLSEGYGEPHERGTLINLYLTQDDLATIINTSRQTINKRLVEWE
                     KLGWIDARYGKIVIININALRQLYADEDE"
     gene            complement(1754..3121)
                     /locus_tag="OO172_003901"
     CDS             complement(1754..3121)
                     /locus_tag="OO172_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5513879.1"
                     /translation="MPSHTDQYLSLAIQKSIRRIIPMVILMFILAYLDRSNIGFAKQE
                     FQLDTSLSDTAYAFGAGIFFIGYALFEVPSNILLYRIGARVWLARIMVTWGLVSAAMM
                     FAHTETVFIFLRFLLGISEAGFFPGVILYLTFWFPQHIRARVTGYFLFGAPLAFIIGG
                     PLSGSLLSLEGSVLSFGLHGWQLMFLVEGLLASAVGIWVFFYLDDHPEKAKWLTPQEK
                     AALSQTLNKEEQVKLAHGPKGAWQALLDVRVLYLCLIWFTVQVCGYGIYFFLPTQIAT
                     LIGTKVGLLVGFVTAIPPLCAAIAVYYVPRISEKLQERRYVASITFMSGAIGIAVSGL
                     ADSIPLVAIVALCVAAAGHLAMQPLYWTFPSSYLGGAAAASGIALINSIGNLGGFVAP
                     NLRVWAEHTFNSAQAGLYLLAMVTATGSLMILTLRHFGIEKTPRTSSTSPNLPASGDN
                     LHHSD"
     gene            complement(3193..4305)
                     /locus_tag="OO172_003902"
     CDS             complement(3193..4305)
                     /locus_tag="OO172_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HCR5513880.1"
                     /translation="MKITKLTTFIMHVPVTKNIIGDSTHSITHWGMPGVMIETDTGLV
                     GYGYTGTHADITTDRLITTIIKDAFGPMLIGEDASEVRYLHRKMSRSSTNIWVGRGGL
                     MQMALSAIDIALWDLKAKMVEQPLWKVFGGSQHSRVLAYNTDCGWLVRNTAELVDDCK
                     KMIFEEGFSAIKMKIGKPDPREDLKRIEAVRKAIGDDVNLMVDANAKWDISTAKQYGA
                     KLADFDITWFEEPMWHDDIASHRTLSGHISTPIALGELLYQFDSFKEFVLAGAVDYLQ
                     PDATRCGGLTAVWEIADLGQAFNLPVTPHHGDMMQAQLHLVMAHPSCSLLEFIPWTLG
                     CFVDPVEVKEGYYSLPTAPGAGTTLRPEALEHFSVR"
     gene            4471..4952
                     /locus_tag="OO172_003903"
                     /pseudo
     CDS             4471..4952
                     /locus_tag="OO172_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
     gene            5013..5446
                     /locus_tag="OO172_003904"
                     /pseudo
     CDS             5013..5446
                     /locus_tag="OO172_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5502..5893
                     /locus_tag="OO172_003905"
                     /pseudo
     CDS             5502..5893
                     /locus_tag="OO172_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    5782..>5893
                     /locus_tag="OO172_003905"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1640 a         1415 c         1448 g         1390 t
ORIGIN      
        1 aattacagga ctggcgaaaa gaacctcgta aaaactattc gaatgaattc aaacttcgta
       61 tggtggaact ggcatcacaa ccaggagcct ctgttgcccg tattgcccgg gaacacgata
      121 tcaatgataa cctcctgttc aaatggctca ggctctggca gaacgaaggg cgtatatcac
      181 ggcgtcttca ggtaacgacc tcttctgaca ctggcgttga attattacct gtagaaataa
      241 cgccggatga gcagaaaaaa actgtggcgg ccattgcgcc gtctttatcc acttccactc
      301 agaccagagt cagtgccagt tcctgcaagg tggagttccg tcacggtaac atgacgctgg
      361 aaaatccatc gccagagctg ctcacggtgt tgatccgcga actgagcggg aggggaagat
      421 gatttcactc ccatcaggta cccgtatctg gctcgttgcc ggcgttaccg atatgcgtaa
      481 atccttcaac ggactgggag aacaggtaca acatgtgctg aatgataatc ccttctccgg
      541 ccacctgttt atcttccgtg gccgacgggg tgacaccgtt aaaattcttt gggctgatgc
      601 tgatggtctg tgcctgttca ccaaacgcct tgaggaaggt cagtttatct ggcctgcggt
      661 gcgtgacggt aagatatcca ttacccgctc gcaactggca atgctgtaaa cgttaacgga
      721 gcgccgtatt gacgcttacg aactgtgtga tccgacatgc cattaggaaa cgtaaggcgt
      781 tcccgatggg cgagtcagta aaagtgaaat ggctggcatc ctggtcgcgt cgcagaaatg
      841 gacaatgccg cagagggact ggcgaatagc aatttattat cgaatttgat gaccgcctgg
      901 acagtcactt ttaagaaaaa gcatgtatac agaatggtaa acaggctcat ccacagccaa
      961 ttactcatct tcatcagcat ataactgcct gagtgcattt atattgatga tcacgatttt
     1021 gccatatctg gcgtctatcc atcccaattt ttcccattca accagccgtt tgttgatcgt
     1081 ttgtcggctc gtattgatta tcgttgccag atcgtcctgc gtaagataaa gattgattaa
     1141 tgtccctcgc tcgtgtggtt cgccgtaccc ttccgacaga ctcactaaac gcttcgccaa
     1201 gcgtgcagaa acagataata gcgtacagtc atgtaacatc gtaaaggcat ttcgaacgac
     1261 tttgcaaagc agcaacgcca gaaaacgatg gagaattgga taatttttca gaagaaattc
     1321 aacatcatga ggcttgatcg ttaacagtgt agttttaccc gtcgctctgg catcgtgtgt
     1381 gcgcggacat ttatcaatca tacctatttc accaaaccag ttccccggcg aaagatagcg
     1441 gaagatgcac tctttaccgt cagcagaaat tgagctcact ttaacggagc cagtaatgac
     1501 tgcatataat ccgagcggct cagcaccacg atagtgaata atctctccat cgttgtattg
     1561 atacaggctg gtcctggaca acatctgttc aataagataa tcaggtagcg cccgaaacca
     1621 actactacta agaaggatac tattgatctc gtccatagaa actatcgatt catgtgattg
     1681 tgctacggcc aatatcttaa ccattgttgt tatcctgatc ttaatcatga ccgtagcaat
     1741 gtagcatttc ctgtcaatca gagtgatgca gattatcccc gctggcaggg agattcggag
     1801 atgtggatga tgtacggggc gttttttcaa tcccgaagtg acgtaatgtc agaatcatca
     1861 ggctaccagt agctgttacc attgccagta agtaaagtcc tgcctgtgcg gaattaaaag
     1921 tatgttccgc ccagacgcga agattcggcg ctacaaaccc ccccagattg cctatcgagt
     1981 taattaacgc aatgccggaa gcagctgctg caccgccgag ataagacgat ggaaatgtcc
     2041 agtacagtgg ctgcatcgcc agatgtcctg cagcggcaac gcacagcgcg acaatagcga
     2101 ccagtggaat actatcggca aggccagata cggcaatgcc tattgcaccg gacataaatg
     2161 tgattgatgc aacgtaacga cgttcctgca gtttttccga tatacgtggt acgtaataca
     2221 ctgcgatggc agcgcacaaa ggtggaatag cggtgacgaa gccaactaat aaaccaactt
     2281 ttgtccctat taaggtggcg atttgtgttg gcagaaagaa gtaaattcca taaccacata
     2341 cctgcaccgt aaaccagatc aggcataaat agaggacgcg tacatccagc agtgcttgcc
     2401 atgcgccttt tggaccatgt gcaagtttca cctgctcttc cttatttaga gtctggctaa
     2461 gcgccgcttt ctcttgtgga gttaaccatt tggctttctc tgggtgatcg tcaagataga
     2521 agaacaccca gatcccaacg gcagaggcca gtaacccctc aacgaggaac atgagttgcc
     2581 agccatggag accaaacgac agtaccgatc cttcaagcga gagtagcgag ccggaaagcg
     2641 gaccgccgat gataaaagct agtggggcac caaacaggaa atagccggtg acgcgtgcgc
     2701 ggatgtgctg ggggaaccag aatgtaagat aaagaataac tccaggaaag aaacctgctt
     2761 cactgatgcc aagcaagaag cgcaggaaaa taaatacggt ctcagtgtgg gcgaacatca
     2821 ttgcagcgga aaccagcccc caggtcacca tgatacgggc cagccagaca cgggcgccga
     2881 tgcgataaag cagaatatta ctgggcacct cgaacagggc ataaccaata aagaagatcc
     2941 cggcgccgaa agcataggcg gtgtcactca gactggtatc cagttgaaat tcttgtttag
     3001 caaagccgat attggagcga tccagatagg ccagaataaa cattagtatg accatcggaa
     3061 tgatgcgacg tatggatttt tgaattgcaa gtgataaata ctgatcggta tggctgggca
     3121 taaatcacct cttagaattt tgtcattatt attttgcccc ggtcattgca taaccgggcg
     3181 atacaagtgt tctcatctaa cgctaaagtg ctcaagagct tccgggcgta aggtagtgcc
     3241 ggcacccggc gcggtgggaa gagagtaata tccctctttg acctcaaccg gatcgacaaa
     3301 gcagcccagc gtccagggaa taaactccaa cagagagcaa gagggatgtg ccattaccag
     3361 atgtaattgt gcctgcatca tatcaccatg atgtggtgtg accggcagat tgaacgcctg
     3421 accgagatcg gctatttccc atacggcggt cagtccaccg cagcgagtgg catcgggctg
     3481 gagataatcc accgcaccgg ccagtacaaa ctctttaaag gaatcaaact gatagagtaa
     3541 ctcacccaac gcaatcggcg tactgatatg cccggacagc gtgcggtgac ttgcgatatc
     3601 atcatgccac atcggctctt caaaccaggt aatatcgaaa tcagccagct tggcaccgta
     3661 ctgcttggca gtgctgatgt cccattttgc attcgcgtcg accatcagat tgacgtcatc
     3721 gcctatcgct tttctgacgg cttcgattcg tttgagatct tctcgcggat cgggcttgcc
     3781 tatcttcatc ttgatggcgg aaaatccctc ctcaaaaatc atcttcttgc agtcatccac
     3841 taactccgcc gtgttacgaa ccagccagcc gcagtcggtg ttgtaggcaa gaacacggga
     3901 atgttgagaa ccgccaaaga ctttccacaa aggttgctcc accattttgg ctttcaggtc
     3961 ccacagggcg atatcgattg ctgaaagcgc catttgcatt aacccaccgc ggcccaccca
     4021 gatattggtc gagctgcgtg acatcttacg gtggagatag cgaacttcac tggcgtcttc
     4081 tccaatcaac atcgggccga aggcatcttt aataatggtg gtgataagac ggtcggtagt
     4141 aatgtctgca tgggttccgg tataaccgta acctacaagg ccggtatctg tctcgatcat
     4201 tacgcccggc attccccaat gagtaatgct gtgtgtggag tcaccgataa tatttttagt
     4261 taccggtaca tgcataataa aggtagtgag tttagtgatt ttcattgcag tttcctgtgt
     4321 ctgtatatct tgattgatgg gcgcaatgta gcaaccggcc ttcagcaggg ctgtcaaatg
     4381 gttgacacat catcagaaga gagaaggaat aattgtattt ttttgaagta gatctcgaat
     4441 ttggaccaca cccagtcgac aagttttcat atagggaact gggccatgac cggagctttt
     4501 caggatgaga tctctgtacg catcatgaag accatgcggc taaagtgatc ctggagtaca
     4561 gcaaactgga aaaaatcgaa aagtattacg aaaaatacgc aaacgcaggc accaatgagt
     4621 ggcacatcga tgaaagtaaa aaagctaccg caaccatgaa tgtagttctg ggtgatgaag
     4681 ataggttaaa atccctcgcg gaagattttg ccagacatta tgaaaaacgc gtagccgaag
     4741 gtttcaccgt aaaaggcaag accatgtttg tgccagccgt gaaattgcct agggttctac
     4801 cgtcaccttg aagctattcg cactgcctgg ttttaagtga aacaagtacc cgatggcatc
     4861 gtcctgactg aacgggagca gaaaaattac cgccttctga aatggtgaaa atggtcatga
     4921 cgcgtggtaa agatgatgac gaggcgcttt atagatttgt gtaattgcct gattttgata
     4981 tgttcaattc aacatcaaat gaaggttaat ttatggacga aaaatagtta cagactctgg
     5041 ctaacgaact ggccaaaaac ctcaaaaccc ctgaagacct cagccagttt gaccgtctac
     5101 tgaaaaagat cagcgttgaa gccgctctca acgcagagat gacacaccat cctgggtatg
     5161 gaaaaatcag tccagaccag gagctaactc ccgcaacggt tattccacaa agaccgttat
     5221 cacaggcgac ggtccactgg aactgcgtac tccgcgcgat cgtgacggta gcttcgaacc
     5281 acaacaggtg aagaaaaatc agccccgtat taccaggatg gataaccaga tcctctcgtt
     5341 gtatgccaaa gggataacca cccgtgagat agccgccgcg ttcaaagacc tgtatgacgc
     5401 tgatgtttca ccggcactga tatcaaaggt cactgatgcc gtgatggtga accgccccgg
     5461 aaatcctgga gactaaactc cctgagaaag aggtaaacag gatgactaaa aatacacgtt
     5521 ttcccccgaa atccgtcaac gggcagttcg tatggttctg gaaagtcagg gcgaatatga
     5581 ctcacaatgg gcagcaattt gctccattgc tccaaagact ggctgtacgc cggagactct
     5641 gcgtgtctgg gttcgccagt atgagcggga taccgggggc ggagatggag ggctcaccag
     5701 tgctgaacgt cagcgtctga aagagctgga acgtgaaaat cgtgaactgc gccgcagcaa
     5761 caatatcctt cgccaggctt ccgcttattt tgtgaaggag gagttcgacc gcctctggaa
     5821 aaaatgatgc cactgctgga taagctgcgt gagcagtacg gagtcagacc ggtatgcagc
     5881 gaactgcata ttg
//
LOCUS       DAJTNM010000186         5775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTNM010000186 DAJTNM010000000
VERSION     DAJTNM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.186"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            121..639
                     /gene="mddA"
                     /locus_tag="OO172_003906"
     CDS             121..639
                     /gene="mddA"
                     /locus_tag="OO172_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5513881.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            636..1085
                     /locus_tag="OO172_003907"
     CDS             636..1085
                     /locus_tag="OO172_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5513882.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1086..1318)
                     /locus_tag="OO172_003908"
                     /pseudo
     CDS             complement(1086..1318)
                     /locus_tag="OO172_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1404..1577)
                     /gene="ortT"
                     /locus_tag="OO172_003909"
     CDS             complement(1404..1577)
                     /gene="ortT"
                     /locus_tag="OO172_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5513883.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1772..1867
                     /gene="yncL"
                     /locus_tag="OO172_003910"
     CDS             1772..1867
                     /gene="yncL"
                     /locus_tag="OO172_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5513884.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2269..3078)
                     /locus_tag="OO172_003911"
     CDS             complement(2269..3078)
                     /locus_tag="OO172_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5513885.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3288..4713)
                     /gene="patD"
                     /locus_tag="OO172_003912"
                     /pseudo
     CDS             complement(3288..4713)
                     /gene="patD"
                     /locus_tag="OO172_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4735..5441)
                     /locus_tag="OO172_003913"
                     /pseudo
     CDS             complement(4735..5441)
                     /locus_tag="OO172_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5431..>5775)
                     /locus_tag="OO172_003914"
     CDS             complement(5431..>5775)
                     /locus_tag="OO172_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5513886.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1511 a         1455 c         1285 g         1524 t
ORIGIN      
        1 ctatcactta aataagtgat actggttgtc tggagattca gggggccagt ctatccgttg
       61 cttcgggctg ttttcgatag tattgataac atcatcatca ttattttatt gaggccagac
      121 atgtccatcc gttttgcccg caaagccgac tgtgctgcca ttgcggaaat ttataaccac
      181 gccgtgttgt atacggcggc tatctggaat gaccaaacgg tggatgcaga taaccgcatt
      241 gcctggtttg aagcgcggac catagcaggt tatccggtgc tggtaagtga agaagatggc
      301 gtagttacgg gatatgcctc gtttggcgac tggcgtagtt tcgatggttt tcgccatacc
      361 gtggaacatt cggtttatgt ccatcccgat catcagggca aaggtctggg gcgtaaattg
      421 ttaagccgat tgattgatga agcgcgggat tgcgggaagc atgtcatggt cgccgggatc
      481 gaatcgcaaa atcaggcttc gctgcatctg caccagtcgc tgggatttgt cgtcaccgca
      541 caaatgccgc aggtaggcac taaatttggt cgttggctgg atctgacatt tatgcagttg
      601 caactcgacg agcgcactga accggacgcg attggatgaa tcagtcgctc acccttgctt
      661 ttttgattgc cgccggaatt ggtctggtag tccagaacac gctgatggtg cgcatcaccc
      721 agacctcctc taccattctc atcgccatgt tgctgaactc actggtgggg attgtgctct
      781 ttgtttctat tttatggttt aaacaaggca tggcggggtt tggcgaactg gtgtccagcg
      841 tgcgctggtg gacacttata cctggcttgc tgggttcgtt ttttgtcttc gccagtatca
      901 gcggatatca gaatgtggga gcggcgacaa ctattgcggt actggtcgcc agccagctga
      961 ttggcggtct ggtgctggat atcttccgca gtcatggcgt accactacgg gcgttgttcg
     1021 gcccaatatg tggcgcaatt ttacttgtgg ttggcgcctg gttggtggcc agacgctcat
     1081 tttaatcaga ggatggtgcc gcctttggtt aactgttcgt ggccgcttcc atatcttctt
     1141 tatgcttgcg accgtgatga gcaatagcgg tgcgcaaacg ctgctgctgg gtgtagcgat
     1201 cttcccgact taactccgcg tcatcgctca gggcgattaa taattcgttc atatgggcaa
     1261 tgacgctctc ttcaatcgcg gcatccacgc gcgcgatgac ttcatccaga tgcgacatac
     1321 tttctcctta gccacttgtg aaatttatcg attaatccaa atgatataag tctgcgtggt
     1381 gccgtcaagc cagtgagtaa gatttaaaac agtgaaaaca acagcgccac cgggaaactc
     1441 atcggccatg tggcccccac cagaaaggcg cttaagaaac ggatgcgttt cttatcgtga
     1501 gaaagaaacc aggtgataag aaatgcgatt gctgccataa ccgcataaaa aacaagcata
     1561 tgttgataga gagacatgta cgggcgtcca gccatatgaa taaaacgcgc gcaaatatgc
     1621 gccatttttt gacgtgcatc aagttttttc accagtttga gaaaaaatca tcaaacagca
     1681 acttgccctt atccatcttc agaatgagta ttattgcgga ttttcttaac ccgtactata
     1741 cacatagggg caatgataaa aggtggcaaa aatgaatgtt tccagtagaa ctgtagtact
     1801 gataaatgtc tttgctgctg ttggtttgtt tactcttatc tctatgagat ttggctggtt
     1861 tatttgatgt agtgattttg cacgcagcac gtactgtcag agaaatcaac tctctgacag
     1921 tacgcaagat cttctcatac tctccaagtt tgcaacatta tccttaatat ttacttcttt
     1981 ttgctggcat ctacattgaa atgagtagaa aacatccccc tactgtcgtc tatacaaaca
     2041 gcttactgat tcaaacggaa tcttgttttc acatttttct atcacgaata ctttatacgc
     2101 gcgggagtct ataaatgctt tatactttca aggaataacg taaatataaa aagatggcgt
     2161 aaagtatatg catggcagga tgtttgcaac ctccttgatg acgctgcccc attgaaattg
     2221 caaaccccgc cctggttatc ttgcccattt atcatgcaaa acagataatt ataggcctct
     2281 gaatacctca gaaagattga attttttata gtccctctct tgaatatttc catttgatat
     2341 caatcgattc atctcttcaa caaaataatt tatatcatca cacttgttat tatccggaag
     2401 atctgagtag ttataattcc accattttat tctcaacagt tcatcaataa catttgaatc
     2461 aaacctaaat ctgattattt ttgcaggaac tcctgcaact atggcatatg ggggcacatc
     2521 tttggtaaca acggaattgg cagcaataac agcaccatca cctattgcga tgccaccttt
     2581 caatacaaca tcatttccta tccagacatc atttcctata atcggatttt caggtttggt
     2641 aatgaatggt ttcaatgtcc agctacgttc aaattcactc atagcaaact tatcaaattc
     2701 accgttataa gtcatcatgt gagtagtaaa acgattcaaa ggatgatttc cacccattat
     2761 ttttacattt gatgcaatac tacaaaatcg acctatacgt atattccctg aaaaatggca
     2821 ggtcggaaat gaaaaagcgc cgacactgga gaaatttttc acaggcattg ttgaatattc
     2881 ttcagcaata acattatctt caattgttat cttgtcacct tttgagaaaa gaaataaatt
     2941 cctttcttta tacgacttct tccccttatg atttaaaaag attttattat ccatacaaaa
     3001 ctcaaagtgt tttttcgtcc atctgaaggt aagggtttta tttttatctt ccgtgaattt
     3061 gtacaattga actttcattg ttaatacttc tacttataaa ttattagttt aataacgata
     3121 ctctaaagcc ttatgctaat caactgtgac aggatgaaaa atcgccatca aacatttagc
     3181 ctaattttta cgccagttat taatgtaatc attctaatac ttaaccgcca ttaaactcag
     3241 aacctaaaac gatagtggcc gtcctataac agccactaac atctaactta atgtttaacc
     3301 atgacgtggc ggacgacggt gtaatcctcc agcccataaa gtgacatatc cttgccgtaa
     3361 ccagaaagtt tctgcccacc gtgcggcatt tcacttacca gcatgaaatg ggtattgacc
     3421 caggtacaac catattgcag ccgtgcgctg acgcgatgcg ccctgcccac atctttcgtc
     3481 cataccgaag atgcaagtcc gtactggctg tcattcgccc agttcaccac ctgttcttcg
     3541 ttgtcgaagg gcgtaacact cactactgga ccaaatacct ctttttgcag gatggcatcg
     3601 tcctgtaatg cgccagccag cagcgtcggc gcatagtaat agccattacc cttgcgcttt
     3661 tcaccgccag tgatcacttt gatgtgccct gtcgctttcg cctcttctac tgccttgctg
     3721 acgcgttcga gatgcgccag cgagcttaaa ggtccaagct ccgtagactc gtcatccggc
     3781 gcaccagatt ttaacgttgc caccgcaggc acccagtttt tccaccagcg tatcgtaaat
     3841 gcctttttgc gcgtagatcc gacaagccgc agtacaatcc tgtccagcat tgtaatagcc
     3901 aaatgtacgt acaccttcga ccactgcttc aatatccgca tcatcaaaaa caatcactgg
     3961 cgctttgcca ccaagttcca tatgagtacg cttaatggac gacgcggtat ggctgatgat
     4021 gtgctcgccg gtggcgatag agcccgtcag cgacaccatc cgcactttgg gatgaccggt
     4081 cagcggatca cccaccgttt tgcctctgcc aaacagtatg ttaatcacgc ctgccgggaa
     4141 gatatctttc gccagctctg ccaacttcaa cgcggtcagc ggggtaattt ctgatggttt
     4201 aagcactacg cagttccctg ccgccagcgc cggagcaagt ttccacgcgg ccatcatcag
     4261 cggataattc cacggtgcga tagaagccac gccccccaac ggatcgcgac ggatcatcga
     4321 agtatgacct tcaaaatatt cacctgccgc cagaccattc agacagcgcg ccgcacccgc
     4381 gaaaaagcga aaaacatcga caatcgccgg gatttcatca ttgaacgcac tatgcagcgg
     4441 tttgccacaa ttacgggatt ccagttcggc aaaaacctga ccattttctt cgataacatc
     4501 cgccagtttc agcaggcatt ccgcacgcgc tttcggtgtg gtttgccccc attcagcaaa
     4561 tgctgcatct gccgcacaca cagcagcatc gacctgctct gcggatgcct cggcaatctc
     4621 cagtaaaacg tcccccgttg ccggattata gacaggctgt ttttcccctt cgccgctaac
     4681 cagttctccg ttaatcagta acttatgttg catagattat tcctgtatca gtggttattg
     4741 accattgtcg ccttcgcggg ttagccacca ggcccccagg atcggcaagg ttgttaccaa
     4801 cataaccagc agtgccacca cgttggttac cggtacatca cgcggtcgcc caagctgatt
     4861 gagcaaccac aacggtaacg ttcgttcatg acctgccgta aaggtcgtaa cgatgatttc
     4921 atcgaacgac aaggcaaacg ccagcattcc tcctgccagt aacgccgaac tgagattcgg
     4981 caacactacg tagcggaagg tttgccagcc attggcccca agatccattg acgcctcaac
     5041 cagactccac gaggtgcggc gaaaacgggc gatgacattg ttaaacacca ccactacgca
     5101 aaaagtcgca tgaccgacca cgatggtgaa aaatcccggc tccagattga tggttttaaa
     5161 ggcggttaat agcgccagac cagtgacaat gcccggcagc gcaatgggca gcagcaataa
     5221 cagcgaaatg gcgtttttgc cgaaaaagtc tcgtctccac agcgcggcag ctgccagcgt
     5281 ccctaacacc agcgcagcgt caggccctgc ggtggaaaac taaacgccgc atcttcagtg
     5341 ttaaacgcat aggcggcgat tatcaggatg ggaaaatgta ggaaaacaac gccgccccag
     5401 gccgccagtt tgaggaaaaa cggtgcgcgt tcagagtgca tcgaacgctc ccagacgttt
     5461 cacgaacgcc aggtacagtg cgatgagaat aatcggcacc agggtgaatg ccgccgccat
     5521 cggcatattg ccaatcgccc cctgctggga ataaaccata ttgccgataa aatatcctgg
     5581 aggaccaacc agctgcggga cgataaaatc gcccagtgtg agtgagaagg taaagataga
     5641 gccagcggca atacccggga ttgccagcgg cagcaccaca tagcgaaagg tttgtcgtgg
     5701 acgtgcgccg agatcagccg acgcctgcaa caatgacggc ggcaaacgct caagcgccgc
     5761 ctgaacgggc aggat
//
LOCUS       DAJTNM010000187         5648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTNM010000187 DAJTNM010000000
VERSION     DAJTNM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.187"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..1113
                     /locus_tag="OO172_003915"
     CDS             <1..1113
                     /locus_tag="OO172_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR5513887.1"
                     /translation="VWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAM
                     AVAIQHGSFIEDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTD
                     QALNAEIQKGEEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQR
                     DPFDWAEKNLQEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARV
                     FIDNYAAIPSESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFI
                     DARGQRPLKVKDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMH
                     DQPFVQGLTACAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(1125..1937)
                     /locus_tag="OO172_003916"
     CDS             complement(1125..1937)
                     /locus_tag="OO172_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513888.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(1941..2726)
                     /locus_tag="OO172_003917"
                     /pseudo
     CDS             complement(1941..2726)
                     /locus_tag="OO172_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            complement(2723..3391)
                     /locus_tag="OO172_003918"
     CDS             complement(2723..3391)
                     /locus_tag="OO172_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5513889.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(3455..4093)
                     /locus_tag="OO172_003919"
                     /pseudo
     CDS             complement(3455..4093)
                     /locus_tag="OO172_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
     gene            complement(4106..>5648)
                     /locus_tag="OO172_003920"
     CDS             complement(4106..>5648)
                     /locus_tag="OO172_003920"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR5513890.1"
                     /translation="TGAIMGSKNLKAIAVEGTKGVNIADRQEMKRLNDYMMTELIGAN
                     NNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPPGNQNTVGFRTYKS
                     VFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGNTCVANFVHTTIFP
                     NGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYSKGVFKRVLPAEEY
                     AEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLAYGSYAIAERWNLGKEYWGYAKNKL
                     WSPFGYPVHHANEASAQVGFIVNCMFNRDCMTHTHINFIGSGLPLKLQREVAKELFGS
                     EDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTVSPLKSRNYRGDLA
                     LEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKPMQTKDMRNEHDLICSWVFDKD
                     PQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQRLGLEDIAADLAAH
                     NLLPA"
BASE COUNT         1439 a         1507 c         1310 g         1392 t
ORIGIN      
        1 gtctggcctg atgaagaaga agcaaccaga acgtattttc ccagcccgtg gtcaggcctg
       61 atggaagcaa aggtcgatgc gtgtaacgtg ggtattatgg gaacatcctt gagcggactg
      121 gatgcggcaa tggcagtggc tattcagcat ggttcgttca ttgaagatga taaacaacac
      181 gtcgtttttc accgcgataa cgcaagtgaa aagctaaata ttacgttgat gtcgcgcacg
      241 ggtattttac ccgaagccga tttctattgc cctattccct gcgagccctt acacatcgtc
      301 accgatcagg cattaaatgc tgagattcaa aaaggtgaag agggcctttt ggatcgggta
      361 tttagattga tagtagagga aatcaagttt gctgatccag actggagcca acgcatagcc
      421 ttagagagcc tgaatgtcga ttcctttgct caagcctggt ttgccgagcg caaacaacgc
      481 gacccatttg actgggcaga aaaaaatctc caggaagtcg aacgcaataa acgagaaaaa
      541 catactgttc cctggcgtta tgtcattctg cgcctgcatg aagccgtaca ggaaattgtt
      601 cctcatctga atgaacacga ccataaacgg ttcagtaaag gccttgccag ggttttcatc
      661 gataattatg cggcaatccc ttcagagtct attcgtcgac tactggcctt acgtgaagcg
      721 gggatcattc atattctcgc cctcggtgaa gactacgaaa tggaaattaa tgagtcgcgc
      781 accgtcctga aaacggaaga caacagctac tcgtttgacg tttttattga tgcccgcgga
      841 cagcgtccgc ttaaagtgaa agatattcct ttccctgggc tacgcgagca attacagaaa
      901 acaggggatg aaatccctga tgttggcgaa gattatacgt tacagcaacc cgaagatatt
      961 cgtggacgcg tagcgttcgg cgcgttgccc tggctgatgc acgaccagcc tttcgttcag
     1021 ggacttacgg catgtgcaga aattggtgag gcgatggctc gggcggtcgt aaagcctgca
     1081 tcccgtgctc gtcggcgtct ttcgtttgat taaaggtaaa gctatcagca ctcaacaaac
     1141 tgatgctgtt cattaagacg atagcggact ccggttcgaa tattgttttc accttcaatg
     1201 gcgacggcga agcgaactct ttcgccgtca tgatatgcca gtgcggcgct tccccctagc
     1261 ccgagaataa ttgagtcgac cacgcccgtg ctcactaccg ttgaattttc accgctggca
     1321 atgatgtgta cattgtcgcc gctgttggca attctggcgt ttgcgccaaa gctggcgatt
     1381 tgcaccaggt caccattact ggcgacatga caccgttcac ccaatgtaca aacgcgtact
     1441 cgcattccgg tattcgcaat ccgactacta tctccagcgc tgcttatccg cgaagaatta
     1501 ccggttacag caacccgtgc gcgttcaccg gagctgccaa tgtgactgtt aaagccgaca
     1561 cttccaatcc gcacgctata acccatactg gcaatttgcg cggcataccc agcggttgcg
     1621 attcgggcgt tatcctcaca acagccgatg cgagcatgat cgtcgctgcg ggcaaactga
     1681 ctgcaaaatg gtatctctcc ggggtctgtg gccagttttt gcatagagga aacttcctga
     1741 tgcgcgaagt tttcatccgc cagccatttt ttccacgcat actcaaccag actctccgcc
     1801 cagtccgtat atccttcctc aatcagtgca tgacgaatat cggcataact accgccgcgt
     1861 gggaaatggc gcaggaacca gcgatacatc accgctccga tacgccattc tcgcaggtct
     1921 gctcgtgtaa tgatcattcg ttagtgccgg tgatagccat cgaccatgct tttaaaggtt
     1981 tcgtgtggcg tacgccccgt ggtgcaaaga taaagatgac cgaagataaa aaagaggctt
     2041 ataaatgcca gagcaaaatg cgcctgcaat aaccagtatc ttacgccagg aaacacatct
     2101 cccacggctt gcggatagag acacaacagc cccgtcaata gtaacaacgg cagcaatcca
     2161 tacatgacac caacataggc gacctgctgt aagggattaa atttagactg tgttgttgcc
     2221 gggaaaggat gttcttcccc ctgcataatg ccaaacaaat aaaatcgcgt ttgttttgcc
     2281 gctcgttcca gccacccctg acgacgaatg cgatagtggt gaccattatc cccaacggca
     2341 ttgatcagca caaagccgag ccagcatgcc agtaacaaaa atcagcaaac ttcatgcacc
     2401 gcaaccagac ttttaaccgc agttgcgccc accatcgcaa agtgatttat cagcccactt
     2461 gccagcaaca atacaaagag taacgcattc gaccagtgcc ataatctgac cgctttgctg
     2521 taaagataaa ccttctcacc atgatcggtg gctgatttct tcacgccacg agcacgaagc
     2581 aaggcgtgca gccccagcac caaccacatc ccaaccagca atactccggc aatgatcaac
     2641 cacaccggcc aaaattcggg agtgaactgt ggcacatagt tcgctaactg gctctgaaac
     2701 tgttcagcat gttgcgacgg gttcatacat tttccttttt aatcaaatgt tgaccgaacc
     2761 ggcgatacag atgcggtttt ccggcaccag gtagctgata ttgatagtat ttattatcct
     2821 gcaaccacgc ctgaatttct gggctatctt cgcgaccaaa gatcaatgcg tgttccggac
     2881 aggcgctgac gcaaatgggc ggaaagcctt ttgccagtcg ggactcagcg cagaaatcac
     2941 atttatctgc cactttagtg acagggttaa ggtagcgaac ctggtacgga caagcgccaa
     3001 tgcaataact gcagccgatg cactgcgatt tttctacccg cacgatcccc tgctcatcac
     3061 gccacgatgc ccccgtcggg catacgtcga tgcacggagc atcttcgcag tgctgacatg
     3121 actggcgaaa aaagtgatac tgcgtttcgt tgtcgttatc ggtgactgga atgtgtgcta
     3181 tcgataaacg gcttccctga gcggggacgt ggttagtttt acggcatgcg cgggcgcaaa
     3241 tattacagcc attgcaccgt gactcgtcat gaatcatggc ataccgaact tccttttctt
     3301 gcctcgtgtt cgctaacaga gaagacgcag aaccagtaaa aaagattact gttcccatcc
     3361 ccagaacaaa tttgcgtcga gtgaaggaca tctgattatc ccctaatgca gtggctgcaa
     3421 ctgtccaccc tatccatgac atctccctga aatttcaggg gttctgaaac tgctctacct
     3481 cgaaccattc gcataagcca taagccttgc cataggtcat aaatcgctcg ctaaacaagt
     3541 acaacaccgc cgtcgatgac tccacctgcc gtatatcggc atattcaggt gcaacatcca
     3601 tcgcggcggt cgcggcttca atctgcgctt cctagaagta atatggcgct tgcatcagca
     3661 tggcgacttt gtaaggacga ggatacgtct ggcactcaaa acgaacggcg tgtgcaatgg
     3721 cgcgacaaat gtcctgctcc acaacttgca aagacatcgc cgcgtagttt tcactcatgg
     3781 cctgcgttga gtaatagtaa tcatcctgac ttcccgtcat acaagcgata tccgcgtagt
     3841 ctccgttctg cttaatgctt tccagcaaaa ctgacagttc ctcttctgca accgaatagg
     3901 gtggcgagag aaacacggtg cgcgccacca gctgcccagc cgcagaatgc tggcgaatga
     3961 actcagccag gatttcagca ggttctggtt cggttaccgt atcaacgggg tgacgcatcc
     4021 cctgcaacca ttgccgcttc gcctcgtcaa cttcgctgac tttcgctaac ggcaattcat
     4081 cctgatggtt catcttaccc attccttacg caggcagtag attgtgtgct gccagatcgg
     4141 cggcaatatc ttccagcccc agacgctgca atgtttcgcg ggttggacaa ccaagctgtg
     4201 ggtcccagcc catttctttg tagaacatcg tgagcgaagc atgcatatcg tcacgatcca
     4261 ttttgtcagt accttcagta aagaccggga tctgcggatc cttgtcgaaa acccaggaac
     4321 agataagatc gtgttcatta cgcatatctt tggtttgcat cggttttacc gtgtaggcac
     4381 gatgcaacgt aaagatacgc tctgcagcta aatctaattt ttcctgagtc atctcttcgc
     4441 cggtgatcgc tttgaagaat ttggcttcaa gcgccagatc gccccggtaa ttacggcttt
     4501 tcagcggcga aacggtcatt ggccagaccc agttgcacag ggtgacggcg ttatgcaaac
     4561 agacccgcaa cagcgaccac tttgcatatt taatttttgc gtcattgatt ggcgtgtagt
     4621 ttttggtttc atcgtaagca tcttcagaac caaaaagttc tttcgccact tcacgttgca
     4681 gtttcaatgg caagccggaa ccaataaagt tgatatgggt atgcgtcatg cagtcacggt
     4741 tgaacataca gttaacaatg aaaccgacct gcgctgacgc ttcattggca tggtgaaccg
     4801 gatagccaaa cggcgaccag agtttatttt tcgcgtagcc ccagtactct ttacccaaat
     4861 tccagcgttc tgcgatggca tatgaaccat aagccaggtg actcagctca cccacacgat
     4921 gcgccagacg gtagtaaaaa tctttaatga agttaacgtc acccgcttcc agttgatccc
     4981 agcgaatttc tgcatactct tcagctggca gaacacgctt gaacacacct ttgctgtaac
     5041 aataagtaaa gtcgcgatgc aactgcccgt agttacacca taggccgtag tcatcgaaca
     5101 aattcagacc aaccaggtta ccaatcacac gaccatcgtc tttatcttca aaatctttcg
     5161 gcccgttcgg gaagatggtg gtatggacaa agtttgctac acaagtgtta ccacctgtgc
     5221 tgggcacgcc aaactctttc acccgaggaa tattcatttg ggtcatacaa cggatcgggc
     5281 aagagtggca gccgctcatt tttactgtgt atttctctgc cgccggtcct aagtcaaaaa
     5341 cggatttata ggtacgaaag ccgaccgtat tttgattgcc tggcggaatt tcacccgttt
     5401 caatcggacc accttcagcc gcgccccaaa acagcccttt acgtgctgtc cagcgtgact
     5461 tgggatctga atactctgcc cacgattgtg gcgtacttgg cacgacatgg ttgttattcg
     5521 caccaataag ttcagtcatc atgtaatcat tgagacgctt catctcctga cgatcggcaa
     5581 tgttgacccc tttcgtccct tcaaccgcaa tcgctttcag gtttttcgaa cccattattg
     5641 cgccagtt
//
LOCUS       DAJTNM010000188         5599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTNM010000188 DAJTNM010000000
VERSION     DAJTNM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.188"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..410
                     /locus_tag="OO172_003921"
     CDS             <1..410
                     /locus_tag="OO172_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5513891.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="OO172_003922"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="OO172_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OO172_003923"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OO172_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5513892.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWFSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1380 a         1402 c         1329 g         1488 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggacagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagaa
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aagggccagt ttggatatc
//
LOCUS       DAJTNM010000189         5543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTNM010000189 DAJTNM010000000
VERSION     DAJTNM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.189"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..638
                     /locus_tag="OO172_003924"
     CDS             <1..638
                     /locus_tag="OO172_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5513893.1"
                     /translation="GACIVLMELMMMVQLWWSVWLCWPVLSLLIVGRGCGGLGAITTG
                     KDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAV
                     LDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALG
                     LSRSALVVAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(692..1297)
                     /gene="azoR"
                     /locus_tag="OO172_003925"
     CDS             complement(692..1297)
                     /gene="azoR"
                     /locus_tag="OO172_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5513894.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1498..5400
                     /gene="hrpA"
                     /locus_tag="OO172_003926"
     CDS             1498..5400
                     /gene="hrpA"
                     /locus_tag="OO172_003926"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5513895.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLSFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1374 a         1353 c         1540 g         1276 t
ORIGIN      
        1 taggcgcgtg cattgtgttg atggagctaa tgatgatggt tcagttatgg tggtcagtct
       61 ggttatgttg gccagtatta tcgctactca ttgttggccg tgggtgcggt gggcttggcg
      121 cgataacaac agggaaagat agtcagggga aactcccgcc cgccgtttac tggctgacat
      181 tgccctggcg catcgggatg tggctatcta tgcgttggtt ttgtcgtcgc ctggagccgg
      241 tgagcaaaat gactgctggt gtttatttaa gggcgtttcc acgacatatt ccggcacaga
      301 atgcggttct ggacgtcacc tttgaattcc ctcgtggacg agcgacaaaa gatcgactct
      361 atttctgtgt accgatgttg gatctggtgg ttccggaaga gggggagctc cgacaggccg
      421 tggcgatgct ggaaacatta cgcgaagagc aaggcagcgt tctggtccat tgcgcattgg
      481 gattatcgcg cagtgcgctg gtagtggcgg catggctgtt atgttacgga cactgtaaaa
      541 cagttgatga agcgattagc tttattcgag ccagacgctc gcatattgtg cttaaggaag
      601 agcacaaagc gatgttgaaa ttatgggaaa acaggtaagt ggattgagat gtggactgaa
      661 tatctacagt ccacatcaag accgtgtccg gttatgcagc aacaatgctg tcgatggctg
      721 cttttgcgtc agactgtgct ttcgctgcca tttccggacc gtatgcgatc ccttcggcga
      781 agacaaattt cacatcggta atgccgataa agccgaggaa cgtggacaga tacggcgtca
      841 ccaggtccgt tggtccatct ttgtggatcc caccgcggct ggtaataacg atggcttttt
      901 tacccgttac cagaccttcc ggaccgttct cggtatagcg gaaagtaacg cctgcgcgtg
      961 caaccaggtc aaaatagttt ttcaactgag ttgagatgtt gaagttatac attggtgccg
     1021 caataacgat aacgtcgtgg gctttcagct cggcaatcag ctcatcggaa agcgccagag
     1081 cttcctgctg acgcggagtc agcggcgcat cgctcggacg cagagcgcca accagttcgc
     1141 catccagtac cggaatcgga tttgcagcta ggtcgcgaac ggtgatttca tcagcggagt
     1201 gcttttcgcg ccattgttca acaaaataat cggacaactg attagactga gagtaccctg
     1261 ccaggatgct ggatttaaga actaatacct tgctcatggt gtttccttat agatgtttga
     1321 atggacgatg ccccgttgct tgctgacact ttattcacaa tcctgccaca gagatagcgc
     1381 aataaatcga agtctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta
     1441 ctctatatct atcatttaaa agaaaattaa tcaggcagac tactgcccac taacgttatg
     1501 acagaacaac aaaaattgac ctttactgcc ttgcaacagc ggctggattc gctgatgctg
     1561 cgcgacagac tgagtttttc tcgccgtctg cattgtgtta agaaggttaa aaatcctgat
     1621 gcacaacagg ccattttcca ggagatggcg aaagagattg accaggcggc agggaaagtc
     1681 ctgctgcgtg aagcggcacg accggaaatc acttatcctg acaatttacc ggttagtcag
     1741 aaaaaacagg acatactcga agcgattcgt gatcaccagg tggtgatcgt cgccggggaa
     1801 accggctccg gtaaaacgac tcagctaccg aaaatctgta tggagctggg gcgcggtatt
     1861 aaagggctga tcggtcatac ccagccgcgt cgcctggcgg cacgaacggt ggccaaccgt
     1921 attgcggaag agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc
     1981 gatcacgtaa gtgataacac gatggtcaag ctgatgaccg acgggatcct gctggcggag
     2041 atccagcaag accgcctgct gatgcagtac gacaccatca ttattgacga agcgcacgaa
     2101 cgcagcctga atatcgattt tctgctcggc tatttgaaag agttgctgcc gcggcgtcct
     2161 gacctaaaaa tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt
     2221 aataatgcgc cgattattga agtctccggt cggacctatc cggtggaagt gcgctatcgc
     2281 ccgattgttg aagaagccga tgacaccgag cgcgatcagt tgcaggcgat ttttgacgcc
     2341 gtagacgaac taagccagga aagccccggc gacattctga tctttatgag cggcgagcgg
     2401 gaaattcgcg ataccgccga tgcgctgaac aagctgaact tacgccatac cgaaatcttg
     2461 ccgctttatg cgcggctttc gaacagcgaa cagaaccgcg tgttccagtc gcacagcgga
     2521 cggtgcattg tgctggcgac caacgtcgcg gaaacctcgc tgaccgtacc ggggattaaa
     2581 tacgtcatcg accccggtac agcgcgtatc agccgctaca gctatcgcac caaagtgcag
     2641 cgtttgccga ttgaacccat ttcgcaggcg tctgccaatc agcgtaaagg ccgctgtggt
     2701 cgtgtgtccg aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg
     2761 gagtttaccg atccggagat tctgcgtacc aacctggcct cggttatttt gcagatgacc
     2821 gcgctggggc tgggcgatat cgctgcgttc ccgtttgtcg aagcaccgga taaacgcaat
     2881 atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga tcaccacgga tgaacaggcc
     2941 agcgcctata aactgacgcc gctcggtcgc cagctctcgc agttgcctgt cgacccacgt
     3001 ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc
     3061 acgtccgcgc tctccatcca ggatccgcgc gaacgtccga tggacaaaca gcaggcatcg
     3121 gacgaaaaac atcgtcgctt ccacgacaaa gaatccgact tcctcgcgtt tgtaaatctg
     3181 tggaattatc ttggcgagca gcaaaaggcg ctttcttcca acgccttccg tcgcctgtgt
     3241 cgtaccgatt acctcaacta tctgcgcgtg cgcgaatggc aggatatcta cacccagttg
     3301 cgccaggtgg tgaaagaact tggcattccg gtcaacagcg aaccggcgga gtatcgtgaa
     3361 attcacatcg ccttactgac cggtttgctt tcccatatcg gcatgaaaga tgccgataaa
     3421 caggaatata ccggtgcacg taacgcgcgt ttctccatct tccccggttc tggtttattc
     3481 aaaaagccgc cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtggggg
     3541 cgcattgctg cgcgtatcga cccggaatgg gtagaacccg ttgctcagca tttgattaaa
     3601 cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa
     3661 gtcactgttt atggtttgcc gattgttgct gcgcgcaagg tcaactacag ccagatcgat
     3721 tcggcgttat gtcgtgaact ctttattcgc cacgctctgg tggaaggtga ctggcagacg
     3781 cgtcacgcat tcttccgtga aaacctgaaa ctgcgggccg aagtggaaga gctggaacac
     3841 aaatcacgtc gccgcgatat tctggttgat gacgaaacgc tgtttgagtt ctacgaccag
     3901 cgcatcagcc acgatgtaat ctccgctcgt cacttcgata gctggtggaa aaaagtcagc
     3961 cgcgaaacgc ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa
     4021 aaaatcagca agctggatta cccgaacttc tggcatcagg gcaatctcaa gctgcgtttg
     4081 agctatcagt ttgagccagg cgcggacgcg gacggtgtga ccgtacatat tccgctgccg
     4141 ttacttaacc aggttgagga aagcgggttt gaatggcaga tccccggtct gcgccgcgaa
     4201 ttggtgattg ctctgattaa atcgttgccg aaaccggtac gccgtaattt tgtgcctgca
     4261 ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc tggagttacc gttgctcgac
     4321 agccttgagc gcgagttacg gcggatgacc ggcgttaccg ttgaccgcga agactggcac
     4381 tgggatcagg tgcccgatca cctgaaaatc accttccgcg tggtggatga caaaaacaag
     4441 aagctaaaag aagggcgctc gctacaagat ctgaaagatg cgctgaaagg caaagtgcag
     4501 gaaacgctgt ccgcggtggc ggatgacggt atcgagcaga gcggcttaca tatctggagt
     4561 tttggtcagc tgccggaaag ttacgaacag aagcgtggca actataaagt gaaggcgtgg
     4621 ccggcgctgg tggatgaacg cgacagcgtg gcgatcaaac tgtttgataa cccgctggag
     4681 caaaaacagg caatgtggaa tggtcttcgc cgtctactgc tgctaaatat ttcctcgccg
     4741 atcaagtatt tacatgagaa gttaccaaac aaagccaagc tggggctgta ctttaacccg
     4801 tatggcaaag tgctggagct gatcgacgac tgtatctcct gcggtgtgga tcaactgatc
     4861 gacgccaatg atggcccggt ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtg
     4921 cgcgccgaac tgaatgacac ggtggtggat attgcgaagc aggtcgagca aatcctcact
     4981 gcggtgttca atatcaacaa acgtctgaaa gggcgggtgg atatgaccat ggcactgggg
     5041 ctttctgaca ttaaagcgca gatgggcggg ttggtatatc gcggttttgt cactggtaac
     5101 ggcttcaaac gtctgggcga cacgctgcga tatttgcagg cgattgaaaa acgactggaa
     5161 aaactggcgg ttgatccaca tcgcgatcgt gcacagatgc tgaaagtcga aaacgtccag
     5221 caggcgtggc agcaatggat caacaaactg ccgcctgcac gtcgtgagga tgaagacgtg
     5281 aaagagatcc gttggatgat agaagagctg cgcgttagtt acttcgctca acaacttggt
     5341 acgccttatc cgatttcaga taagcgtatt ttgcaggcga tggagcagat tagcggttaa
     5401 ccctgctatt tgcctaataa atctaaaacc cggtaagcat ttagcgccgg gttttttatt
     5461 aattctaaaa cggtaaggac aaaaattcag gaattcagaa aaatacaatt ctcagctgca
     5521 agatgaataa tgtttatcta cag
//
LOCUS       DAJTNM010000190         5538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTNM010000190 DAJTNM010000000
VERSION     DAJTNM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.190"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO172_003927"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO172_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5513896.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO172_003928"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO172_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5513897.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>5538)
                     /gene="mdtF"
                     /locus_tag="OO172_003929"
     CDS             complement(2659..>5538)
                     /gene="mdtF"
                     /locus_tag="OO172_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HCR5513898.1"
                     /translation="SSTSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAV
                     QQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGS
                     EYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQT
                     RLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAG
                     ANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLV
                     MYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVV
                     VENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQF
                     SITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDS
                     TRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTT
                     KVLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTA
                     IIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLS
                     LAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGSSYVNDF
                     LNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYN
                     GIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYA
                     ISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIG
                     LSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHG
                     AGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1464 a         1288 c         1302 g         1484 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atatcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaaaaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaaga
//
LOCUS       DAJTNM010000191         5514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTNM010000191 DAJTNM010000000
VERSION     DAJTNM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.191"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..795)
                     /locus_tag="OO172_003930"
     CDS             complement(<1..795)
                     /locus_tag="OO172_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5513899.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAK"
     gene            1041..1667
                     /gene="gstB"
                     /locus_tag="OO172_003931"
                     /pseudo
     CDS             1041..1667
                     /gene="gstB"
                     /locus_tag="OO172_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(1664..2778)
                     /gene="yliI"
                     /locus_tag="OO172_003932"
                     /pseudo
     CDS             complement(1664..2778)
                     /gene="yliI"
                     /locus_tag="OO172_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2889..3272)
                     /gene="bssR"
                     /locus_tag="OO172_003933"
     CDS             complement(2889..3272)
                     /gene="bssR"
                     /locus_tag="OO172_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5513900.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3485..4810
                     /gene="rimO"
                     /locus_tag="OO172_003934"
     CDS             3485..4810
                     /gene="rimO"
                     /locus_tag="OO172_003934"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5513901.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4857..>5514)
                     /locus_tag="OO172_003935"
     CDS             complement(4857..>5514)
                     /locus_tag="OO172_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5513902.1"
                     /translation="YLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINN
                     HFERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLF
                     DVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEE
                     LLSILKEIVHFQVGSINLSTSIGVAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ
                     "
BASE COUNT         1393 a         1431 c         1314 g         1376 t
ORIGIN      
        1 tttcgccccc agcactacgg agattaaacg catatcaccc tgggtagccg aagcaaccag
       61 attatatccc gcacctgccg tggttcctgt cttcatgcca tcaacattca gattgctgct
      121 ccacagcaga cggttacggt taggctgacg aattttgttg aaggtgaact ctttctcttt
      181 atgaatggcg tactcttccg gcacatcgtg gatcaatgct ttacccagca atgccatatc
      241 gcgcgcggta ctgaactgcc ccggcgcatc caggccgtgc accgtctgga aggtagtgtt
      301 ggtcagcccc agttttttgg cataaccatt catcagacca ataaaggact cctggctccc
      361 ggcaacgtaa tcggccagcg caatacaggc gtcattaccg gactggataa tcacaccttt
      421 gttcaagtct gccaccgaaa cctgatcgcc cggtttgagg aacattaccg atgaaccacg
      481 cagtgccgga tttcccgtcg cccaggcatc tttaccgacc gtcaccatat cggtgagttt
      541 aatcttatcg gccttaagca cctgcccaac cacatagctg gtcatgattt tagtcaggct
      601 cgcgggatcc agtttctcat ccgcattgcc ttctgccagc actttaccgc tggcgtaatc
      661 cattaaaatc catgcacgcg catccacgct cggcgcttca acggtttgtt cggccgcgaa
      721 tgccgttggg gcaaaaagga ataaaaatgc agaacccgct gcaagagcac gaaggagaga
      781 ggagtattgc gtcataaatg ccacccgagt atccattcaa aaaaatacgt tatatcgccg
      841 tatgataagt tcggtgatta ataacgcaca cacaaactta aagaaaccgt aagttggtaa
      901 agtttttaaa gtttatgcaa tatcctgccg cttcgctgct tctggtgtca tttttttgcc
      961 agctgttgct taaaagttaa ctttacttca ccatcggagt cctgttcagt ctgtggctgc
     1021 ctgtcggaaa aggggtgagt atgattacgc tgtggggtcg gaataattca actaacgtaa
     1081 aaaaagtatt gctgacgctc gaataactgg aactacctta tgagcaaatt ctcgcgggcc
     1141 gtgagtttgg gataaatcac gatgctgatt ttctggcgat gaaccctaac gggctggtgc
     1201 cgttgttgcg tgacgacgaa agtgatctca ttctttggga atcaaacgcc attgtccgct
     1261 atctggcggc acagtacggg caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg
     1321 aagcggaaaa atggatggac tgggcaaacc agacgctcag taatgctcat cgcgggatcc
     1381 tgatgggatt agtcagaaca ccaccggaag agcgcgatca ggccgccatt gatgccagtt
     1441 gcaaagagtg cgacgccctg tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt
     1501 tctctggcga cgagtttggt gtgggcgata tcgctatcgc accgttcatc tacaatttgt
     1561 ttaacgttgg cctgacctgg acaccgcgtc caaatctgca acgctggtat cagcaactca
     1621 ctgaacgccc cgcagtgcgc aaagtggtga tgattcccgt tagctaattg cgtgggctaa
     1681 ctttaagtaa ttccccactg gactcgtcgg tgagaacgta taaataaccg tcgggtccag
     1741 tgcgaacatc acgaattcgc tgccctctgt ccgttaaaat acggccatct tctgtcactt
     1801 tgtcgccgtt gacgctcatc acaatgacat ctttatcttt cagcgcccaa taaataattt
     1861 ttgctgccac tgggggaatt tatcgctgtt atagaaggcc atgccgctca cagcgggcga
     1921 atctttccag taaaaaacag gttgctcggt cccggcgacg atctcccctt tcgcttccgg
     1981 tatcttaaag cctgaatagt tgattcccca ggttgccagc ggccagccgt agtttttgcc
     2041 tttttgcggg atattaattt catcaccacc gcgcgggcca tgttcattca gccacagtgc
     2101 attactccac ggattcatcg ccattccttg cggattacga atgccataag accagatctc
     2161 gccgcgcgca ccggattcct ttataaaagg attatcatcc gggatttcgc cctggtcggt
     2221 cagacgcacc agtttgccct gtaatttatc cagatcctgc gccgtcgggc gctgattgtt
     2281 ttcgcccaga gcaataaaaa gataaccttt accgtcgaat accagccgcc cgccaaaatg
     2341 gttgccggta gacagttttg gcatctggcg gaagacggtg cggaagtcgg tcacttttga
     2401 gagatcatca cttaagcggc cataacccac agcagttccg gctttgccat catcgccaac
     2461 ttcggaataa cttaaccaga tgcggcgaga ctgagcaaaa tcaggcgcta aaaccacgtc
     2521 cagcaggccg ccctgcccgt gcgcccaaac gtccggaact ccggaaagcg gcgcagataa
     2581 tccttttcct gcttgccagt gacgcaactc gccgccgcgc agagtgatta acataccgtg
     2641 attatcgggt aaaaaggcca gtgcccaggg atggtcgagt ttgtcttgca gtacttcgac
     2701 atttaccgtt gcaggagccg cccagagagc ggaagaaaga caaataaggg gcacaaggaa
     2761 aaaggattgt cgatgcataa cgcctccgga ttgactctgg cttaaagcgt agtcagtgga
     2821 ggagataaca aattcatttt tacaaaaact taaacatgaa gggggagacg ctttctcccc
     2881 cttagttttc aggccttctc aagcatggcg tgcttctgca ggctctggat actcagcgtt
     2941 aagctcatca gacaattttc aagcttatcg gcgttgacgg taataacagt cgggcaatca
     3001 tggtgcccac tcatcaaaca tactgcggct gtcgctaatg cttcttcagc atgatgaaga
     3061 gcactccact cttcctgatc cagatgaaga ttcaaccgca gcgatttatc gtgcagttcg
     3121 cgattcagtt taaaaaagtt atctcgtaga tgattgcttt cgctgacgga catgtatcct
     3181 tttgccttcc tcagttgcac gtatgcagcg aggtcaacgc gtgcgtcgat caaccggttc
     3241 agcagatcga ttcgctgtct gtcaacgaac atacttcgat cctcctcttt ccagccttgt
     3301 tgcaattaaa tagtatggct attttttgag caaaatgctg tctaggatca attcttcacg
     3361 ccgaattaac aaaatttaca tttccttcgc ctccgccagc gaatgagatt gcgatacaaa
     3421 ttttatccag aactctgtag aatccctgcc ctgacctttc ctgtaactga atatttttta
     3481 agctatgagc aaagtaactc cccagccgaa aatcggcttt gtttcccttg gctgtccgaa
     3541 aaaccttgtc gattcagagc gtattctcac cgaactccgc actgaaggtt atgacgtggt
     3601 accgagctat gacgatgcgg acatggtgat cgtcaacacc tgcggcttta ttgacagcgc
     3661 ggtacaagaa tcactggaag ccattggtga agcgttgaat gaaaacggca aggtaattgt
     3721 gaccggttgt ctgggggcaa aagaagatca gatccgcgaa gtccacccga aagtgctgga
     3781 aatcaccggg cctcatagct atgagcaggt tctggagcac gttcatcact acgtgccaaa
     3841 accgaaacac aacccattcc tgagcctggt gccagaacaa ggtgtgaaac tgacgccgcg
     3901 tcattatgcc tatctgaaaa tttcggaagg ctgtaatcac cgctgcacct tctgcattat
     3961 tccgtctatg cgcggcgacc tggtgagccg tccgattggc gaagtgttaa gtgaagcgaa
     4021 acgtctggta gatgcgggcg ttaaagagat tctggtgatc tcgcaggata cttccgccta
     4081 tggcgttgat gttaaacatc gtactggctt ccacaacggc gagccggtaa aaaccagcat
     4141 ggtcagcctg tgcgaacagt tatcgaaact ggggatctgg acacgtctgc actacgttta
     4201 cccttatccg catgtggacg acgtcatccc actgatggca gaaggcaaaa tcctgccgta
     4261 tctggacatt ccgttgcagc acgccagccc gcgcattctc aaactgatga agcgtccggg
     4321 ttctgtagat cgccaactgg cgcgcatcaa acagtggcgc gaaatctgcc cggaactgac
     4381 cctacgctca acctttattg tcggcttccc tggcgagacg gaagaagatt tccagatgct
     4441 actcgacttc ctgaaagaag cacgtctgga tcgcgttggc tgctttaaat acagcccggt
     4501 tgaaggtgca gacgccaatg ccctgcctga ccaggttccg gaagaagtga aagaagaacg
     4561 ctggaaccgt ttcatgcagc tgcagcagca gatttccgcc gagcgcctgc aagagaaagt
     4621 gggccgtgaa attctggtga ttatcgacga agtggacgaa gaaggcgcga ttggtcgcag
     4681 catggcagat gcaccggaaa tcgacggcgc ggtttatctc aatggtgaaa ccaacattaa
     4741 gccgggtgat atcctgcgtg tgaaagtcga gcacgccgat gagtacgatt tgtggggtag
     4801 ccgggtttaa ttaattcagg ccgggcaaaa tcatttgtcc ggcctgcgaa taatgattac
     4861 tgccagctaa tctgtgccct gccgtttttc ttactcttat acagccgctc atccgccagc
     4921 attttcaagc gttcgacggt agtacattta tttgaatgcg ctacaccgat actggtgctt
     4981 aaattaatgc ttcccacctg gaaatgaacg atttctttta gtatcgatag caactcttcc
     5041 agcgtgccgc cagtataaat caccgcaaat tcgtcacctc cgaagcggta gactttacct
     5101 ttatctaccg ggatctgtga tttaaggatt ttcaccactt tcatcaacac ttcatcgccc
     5161 agcaaatgac cgaaggtgtc gttaatgttt ttgaatttat cgacgtcaaa cagacacaaa
     5221 taaccgcctt tttccggcag cgcttttaaa tcttcatcaa aggattttct gcccattgct
     5281 ttggtcaggt cgtccaggaa cgccgagtta tacatactgt agagtttttt gacatcggca
     5341 gccgaaaaga aaagcccgcc atccagtacg cgttcaaaat ggttattaat gatggcttct
     5401 ttctccacaa taccacgatt aatcactctg cgtaccagat agaggcaact ggcacccgat
     5461 atcactgcca ccaatgcggc aaagaaaata atcaatgtac aaacccgcaa ataa
//
LOCUS       DAJTNM010000192         5455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTNM010000192 DAJTNM010000000
VERSION     DAJTNM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.192"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(286..570)
                     /locus_tag="OO172_003936"
     CDS             complement(286..570)
                     /locus_tag="OO172_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5513903.1"
                     /translation="MLDMAGLRADILSPVKETGTLLGVVSSQAAELCGLKAGTPVVVG
                     GGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQLPITPFRMPS"
     gene            604..1410
                     /locus_tag="OO172_003937"
                     /pseudo
     CDS             604..1410
                     /locus_tag="OO172_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            1422..2445
                     /gene="lsrB"
                     /locus_tag="OO172_003938"
                     /pseudo
     CDS             1422..2445
                     /gene="lsrB"
                     /locus_tag="OO172_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            2472..3347
                     /gene="lsrF"
                     /locus_tag="OO172_003939"
     CDS             2472..3347
                     /gene="lsrF"
                     /locus_tag="OO172_003939"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5513904.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PGVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            3371..3661
                     /gene="lsrG"
                     /locus_tag="OO172_003940"
     CDS             3371..3661
                     /gene="lsrG"
                     /locus_tag="OO172_003940"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5513905.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3700..4458
                     /gene="tam"
                     /locus_tag="OO172_003941"
     CDS             3700..4458
                     /gene="tam"
                     /locus_tag="OO172_003941"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5513906.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(4462..5376)
                     /locus_tag="OO172_003942"
     CDS             complement(4462..5376)
                     /locus_tag="OO172_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5513907.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNELPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         1378 a         1304 c         1455 g         1318 t
ORIGIN      
        1 agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc aggctatttc
       61 tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc tgaacgaata
      121 acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat atacattatg
      181 cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata gacatgtccg
      241 cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg acggcatacg
      301 gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct tattatccgt
      361 gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc caaggcaacc
      421 aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac cgcagagttc
      481 cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag gagaaagaat
      541 atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg tggttagatc
      601 aagatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt actgtttcaa
      661 agtggtgtgc cgatgccgct ggcgatactc ctgaccttac tgctcggcgc attgtgcggg
      721 ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat tacgcttggc
      781 acgctgtatc tgtttgccgg aagcgctctg ctgctttccg gtatggccgg agcgacgggg
      841 tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct ggatgtgctg
      901 ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg gctctggctg
      961 cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg cgtggcgctt
     1021 tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac ggggctggcg
     1081 tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc cgatctcggt
     1141 gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa tatttatggt
     1201 ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata tttgcagcaa
     1261 ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg tgcgctgctt
     1321 atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa agagtggctg
     1381 gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt catcgcttta
     1441 agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat gtgcaggccg
     1501 cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc agcggtggca
     1561 acggcgcaca acaagcgggt aaagagctga gcgttgatgt gacctacgac gggccgacag
     1621 aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa ggttataacg
     1681 ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa acgcgccatg
     1741 caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg ccgctcttac
     1801 tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat ggcggcgcgt
     1861 caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac cgttacggac
     1921 caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc aggctgggaa
     1981 attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac cgcagaagga
     2041 atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa cgccctgccc
     2101 gctgccgcac aagccgcaga aaacctgaaa aatgacaaag tagcgattgt cggattcagt
     2161 acgccaaatg tgatgcgccc gtatgtagag cgcggcacgg tgaaagaatt tggactgtgg
     2221 gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgcattatt gaaaaaagga
     2281 tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggttga agtctcgcca
     2341 aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact gttaccggag
     2401 cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc attacttaac
     2461 cggagtaagt tatggcagat ttagacgata ttaaagatgg taaagatttt cgtaccgatc
     2521 aaccgcaaca aaatatccct tttaccctga aaggttgcgg tgcgctggat tggggaatgc
     2581 agtcacgctt atcgtggata tttaatccga aaacgggtaa aactgtgatg ctggcctttg
     2641 accatggtta ttttcagggg ccgactaccg gacttgaacg cattgatata aatatcgccc
     2701 cgctgtttga acatgccgat gtattaatgt gtacgcgcgg cattttgcgc agcgtagttc
     2761 ccccggcgac caataagccg ggggtactgc gggcgtcagg tgcgaactct attctggcgg
     2821 aattaagtaa tgaagccgtg gcgttatcga tggatgatgc cgtgcgcctg aacagttgcg
     2881 cggtggcggc gcaggtttat atcggcagcg aatatgaaca tcagtcgatc aaaaatatta
     2941 ttcagctggt cgatgccgga atgaaagtgg gaatgccgac catggccgtg actggcgtgg
     3001 gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc gccgctgaaa
     3061 tgggggcgca aattatcaaa acctattatg tcgaaaaagg ttttgaacgg attgttgccg
     3121 gatgtccggt acccattgtt attgctggcg gtaaaaaatt accggagcgc gaggcgctgg
     3181 aaatgtgctg gcaggctatc gatcagggcg cttctggtgt ggatatgggg cgtaatattt
     3241 tccagtctga ccatccggtg gcgatgatga aagccgtaca ggcggtggtt caccataacg
     3301 aaacggctga tcgggcatat gaactctatc tgagtgaaaa acagtaactg cggatttaag
     3361 gagaagaatt atgcacgtca cactggttga aattaacgtt catgaagaca aggttgacga
     3421 gtttatcgaa gtttttcgcc agaaccacct gggctctgta caggaagaag gtaatttgcg
     3481 cttcgatgtc ttacaggacc cggaagtgaa ttcgcgcttt tatatttacg aagcctataa
     3541 agatgaagac acagtggcat tccataaaac cacgcctcac tacaaaacct gtgtcgcgaa
     3601 actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt tgatgccgtg
     3661 aggctacaat tggggtaacg cgctgacggg agtaaaaaaa tgtctgactg gaacccctct
     3721 ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct tgccagagtg
     3781 ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa cagcaccgcc
     3841 ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc tccggcgatg
     3901 attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga catccgcaac
     3961 tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca atggctgccc
     4021 gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg cgtgctggca
     4081 gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga agttgcttgg
     4141 gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc ttactacgat
     4201 attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta tcaccagatg
     4261 ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc gtggttacag
     4321 gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat gctggaagag
     4381 cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct gtttattgtt
     4441 gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa gaatttttgc
     4501 tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt tgcagatggc
     4561 atccagcggt aactcattgt tttcagtacc gaacggatct tccagttctt ccgccagaca
     4621 atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca tgtaatgcag
     4681 gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac gatgcaaaat
     4741 cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg ccagaaccgc
     4801 tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa gctgtccatt
     4861 gcggcgctga accgccaacc attctcccat tattaacaag atacggttag ctggagagtt
     4921 cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca cttccgcctg
     4981 tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca gcctggcaaa
     5041 ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta acgaccgtga
     5101 ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt acccggcatt
     5161 attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg gtgcgagggt
     5221 gaatttgatg cccagatgcg tgtaccaggg cagcatgaat ataacagcaa tagaaaagag
     5281 aaaattgagt agtaagcgcg aggatatctt ggataatact gagccgtgcc agacaaaaat
     5341 acggcgcagc cagtgttgtt gtggacgaac aatcatggtt atcttcaggc gtggaaaagt
     5401 cgccctatta aacgtgatta cgatcacatt ctcaagacgc tacttacaaa ttacc
//
LOCUS       DAJTNM010000193         5428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTNM010000193 DAJTNM010000000
VERSION     DAJTNM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.193"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..90)
                     /locus_tag="OO172_003943"
     CDS             complement(<1..90)
                     /locus_tag="OO172_003943"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5513908.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNAR"
     gene            complement(252..3560)
                     /gene="icsA"
                     /locus_tag="OO172_003944"
                     /gene_synonym="virG"
     CDS             complement(252..3560)
                     /gene="icsA"
                     /locus_tag="OO172_003944"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5513909.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGSS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4089..5291
                     /gene="virA"
                     /locus_tag="OO172_003945"
     CDS             4089..5291
                     /gene="virA"
                     /locus_tag="OO172_003945"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5513910.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1684 a         1224 c          900 g         1620 t
ORIGIN      
        1 acgtgcatta gtttctgcaa taccagtaat ctgaggcgca taaccacgaa tatttggcgg
       61 tagcattcgt tcatcgcttt ctaatgcaat tcctgtgtaa cgccaagcaa cagcgcattc
      121 tggcgcatga tctggtaaac acgtttggca ttgatcgcag gcataccatc atgtgcacaa
      181 aaacccaccg agcttttaca cggtgggtcc cagagaaatg caggacatca acacgccctg
      241 catttttatt atcagaaggt atatttcaca cccaaaatac cttgggtatc tctgtaactg
      301 ttattaccca attgctgaga tacgtttccc cagatgctta ggttatctgc aagacgcccc
      361 cccacaccag ttttgagttc aatcacatta cggttgccta tctggtgatt tgacatgcca
      421 ttcataatta caccatattg ctttgagctc catttccaat tcacttccaa aaatggttcg
      481 aacttacgaa gagtctcctt atcgatacaa ctctgactaa ttaaggatgt acgcaatccc
      541 aattttgctt gaatattatt gaccccagag gatgtgacca tagtgccatt gtgttcaata
      601 tagtcgtttg cttttacccc atgcctagtt atctggactt gtggattgag atacaatgcg
      661 ttgtcaatat tattatgagc agtccagcgt atggttgggt agatatagcc agcttccaaa
      721 gctcctgtta ttcctttgct ggaatatttc tcaccagtta acccatctcc tttgactgta
      781 ttattaaacc aactgtactg caaccaagca tccatatata gacctgttcg ttctttttca
      841 tcctgaaacc aagaactgta caaaccggca gtataaccgc tcacagttcc tcgtgaacta
      901 taactagtat acttattatg agttttgcta tcctgatatc cataagctcc cataataccc
      961 acatgcaagc catcatgatc agtgaagttt gtcttcaaaa tatctccacc aatctgataa
     1021 accatcctgt tagctgtggt ttttaattgt ctatcagcta aattatgctc attatgtcca
     1081 ccaacagtac gaatccacat ggtggtttcg ctgagctgac ctgtagtagg atcaacaata
     1141 gtgctaccgt gtcggtcata gagagaagga ctaaacatcg tgttcattgc tatcaggtta
     1201 gcaatataac tgccagcttc agggcgccat gtgtgaatac taccttcacc caaattaagt
     1261 gaagatacaa gctgtgaact agctcggcga ctactcattt gagtagactc ttgattgctc
     1321 atctgttttg attcttgatt atccatctga ctagttagat accacttatt ggtattcagt
     1381 ccagatacag tgccctgttt caggcgatag tcataactac cagcaacaat acggcctttc
     1441 tgaataaaag cattcttatc agaagaatct gtcgaaataa tttgaacacc ttcaagagtt
     1501 ttatctccag agcctccctc atttgtcact gcaacatagg taataccaga cgcatggcct
     1561 gcaatttcca gccggtcagt tttactgttg tcattgccga gaaccgcgcc caaagataat
     1621 attccgccct ttccatgcca attacctttc tgaacatagg tctgaccgac atttgacgaa
     1681 ctattattga gaataacatg accgtttttc tccagagtca tgttacctgt gacaatgacg
     1741 gggtcaggca aaaaaggggc attaatatta ttgattagca cagtcccact atttaataaa
     1801 gtgttaacag tttggttcat gcctgaaaaa ttcaaggtag cacctttatt aacaataaca
     1861 ccagcggtac gtgtcatagc ttcaactgtc cccattttta gaatgcctgc attgatgttg
     1921 gtgaaaccag agtaggtatt tttttccgcc aaaatgagag ttccctcccc tgattttgtt
     1981 agggattttc cgtcccagct atttatatta tgatggctga gattatctgc cagaatagta
     2041 ctaacagtaa gttcagcgtt ttctttcaga ttgaactctc catgactgtc gccatctgta
     2101 tcattccagc tcaaaccgta gatcaattta ttctggtcac tgatatagct ttttgtatag
     2161 tcaatagcac cagtagtgag taacggattg atgatagata tattagaaac actggaacct
     2221 gttatccctt tatctgcact tagcagttta tagtccttct gcgcccagtt atttataata
     2281 gcttccactt ttacattgtt gccaaatgac atatttccag aagcttccag actactgatt
     2341 ccagctaaat ttaaagaact gttctcacca aaagaaacgt cacctgaaac agtgaatttg
     2401 ttacctgata gtgaaacagt tgtacaatca ccagcacata aatcaccttc aatagttggt
     2461 gtggtcccag tgtaatcatt gttgataatt ttcagaatgg atgaattgtt aacctgtaca
     2521 tcaccagtaa tgacagaacc ttcatggagt attaatgaat tgtttccacc agtgatgtgg
     2581 atagcattac ctgctacatt ttgtgttcca tcatcttctt tacctgaaat tatatatccg
     2641 ctattagcaa tggtcatatt gctaccattt atagcatccc cccaatgtcc accattaccg
     2701 cctaaaatag ctccattgtt gattacagac aggttatctc ctgtgatagc attaccaccg
     2761 tagccatcat aaccataacc gtaagcagca cctccgttgc ctccagcgaa cgtgccctta
     2821 ttgattacag agaggctgct tcctgtgatg gcatcacctc cattaccgcc atcaccctca
     2881 ccataattgt tacctccatt accaccggaa atagtccctt tgttgattat gggtagattg
     2941 actcctgtga cggcatcacc tccattcccc ccatcactat cactataact atcgccacca
     3001 tgtccacctg aaataatttc tccatttatt ataaacagat tgtctcctgt aacagcctca
     3061 ccaccatcac cgttatggtc agcaccgcta ccgccgctac caccaagaat catgccttga
     3121 ttgattatag acaagtcaga tccggtaata gagtcaccac cattaccgcc acaactatta
     3181 ccatcattat tatcaccagc accaccatga cctgcgctaa tcataatatc agagttaatc
     3241 cgtagcttag ttctagatgc atgagagggg ttggaagaag ttatatacca cgcatccatt
     3301 ccatcttcac cagctcccaa gggagaaagt ccatcaacag gtgttaataa tttttcataa
     3361 ttgtcctctg aaaaatgaag ttcttgagta cccgaaagag gagtagcaaa agctattggc
     3421 cccccgagca acagggatgc accaacaaca aaaggagaaa aagacaattt gcatgttttt
     3481 tcctttaccg taggtaattc tccggccccc ccctgtgaac attgggtcat attacaaaaa
     3541 aatttgtgaa tttgattcat gcactatatt atcagtaagt ggttgataaa cccctgaaaa
     3601 gattccgttt tatccggaat aaagggacga tatatgcaaa aacatattaa acaaagcctc
     3661 aaccaaaaca tgagagctgt gttatatatc aatgcaacgg gagaaattta cctgtgtctt
     3721 gctttgaaaa aaagagggga aaagctatat gttcgataat aaattaataa ttttactatg
     3781 atagtctgct tcagaccgca acttttcagt cctttctgta cgcttgccca aagtttaacc
     3841 actttgtttt tctatacacc caatggttct atctgcccct tgatgttaat ccccaaagcc
     3901 aataacatga atgtattcat atcacaactt tcctaccaga ctctccaatg caataactgc
     3961 ataattgttt ttagtggaaa tgtatcgttt tcttaaagag aagaataaca ttccatttta
     4021 ttatgttccc aatttaatta tattaatata tattggcatt aataggaaaa tacatcagga
     4081 gaaatcaaat gcagacatca aacataacta accatgaaag aaatgactca tcatggatgt
     4141 caactgtcaa atcaactaca gaagtaagtt ggaataaatt gagtttttgt gacgttttac
     4201 tgaaaataat aactttcggc atatattctc cacacgaaac tctagccgaa aaatatagcg
     4261 aaaaaaaact aatggatagt ttctcaccat cactatcaca agataaaatg gacggagagt
     4321 ttgcccacgc aaacatagat ggcatttcaa tacggctatg ccttaacaaa ggcatatgtt
     4381 cggtttttta tcttgatgga gataaaatac aatccactca actttcatca aaagaatata
     4441 ataacttgtt aagttcactc ccccccaaac aattcaactt aggaaaagta catactatta
     4501 cagctccggt atcagggaat tttaaaactc acaagcctgc accagaagtt attgaaacag
     4561 ccattaattg ctgtacatcg ataataccaa acgatgatta cttccacgtt aaagacactg
     4621 attttaatag tgtttggcat gatatttaca gagatattcg agcatctgac agcaatagta
     4681 caaaaatata tttcaacaat attgaaatac cactaaaatt aattgcagat ctaattaatg
     4741 agttaggaat taatgaattc attgatagca aaaaagagct tcaaatgcta agttacaacc
     4801 aagtaaataa aataatcaat tcaaacttcc cccaacaaga cctatgtttt caaacagaaa
     4861 aacttttgtt tacttcactc tttcaagacc ctgcatttat atctgcacta acatctgcat
     4921 tctggcaatc tcttcacatc acgtcttcct ctgtggaaca catatatgcc caaattatgt
     4981 ccgaaaacat agaaaacagg cttaatttca tgcctgaaca acgagttatt aacaattgtg
     5041 gacatattat aaaaatcaac gcagttgtcc ctaaaaacga cactgcaatc tctgcctctg
     5101 gggggagggc ttacgaagtt agctcatcaa tattaccatc gcacataaca tgtaatggtg
     5161 ttggtattaa taaaatagaa acaagctatc tcgttcatgc tggaacactt ccaagcagtg
     5221 aagggcttcg caatgctatc cctccagaaa gtcgtcaagt atcatttgcc atcatatctc
     5281 ctgatgttta agccaatatt atattggctg ccagactgta aaaaaacgtt cttcagaaca
     5341 gctctataaa ctattaaacc aaagctaaaa ggatggggac caatagaatc tcagcccatc
     5401 ctttttatat cattatgaac cgccccgg
//
LOCUS       DAJTNM010000194         5403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTNM010000194 DAJTNM010000000
VERSION     DAJTNM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.194"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..1220
                     /locus_tag="OO172_003946"
                     /pseudo
     CDS             1..1220
                     /locus_tag="OO172_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(1256..1459)
                     /gene="ydfZ"
                     /locus_tag="OO172_003947"
     CDS             complement(1256..1459)
                     /gene="ydfZ"
                     /locus_tag="OO172_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5513911.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1637..2323)
                     /gene="ydfH"
                     /locus_tag="OO172_003948"
     CDS             complement(1637..2323)
                     /gene="ydfH"
                     /locus_tag="OO172_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5513912.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSNWFSEE"
     gene            complement(2412..3158)
                     /gene="ydfG"
                     /locus_tag="OO172_003949"
     CDS             complement(2412..3158)
                     /gene="ydfG"
                     /locus_tag="OO172_003949"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5513913.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3295..5340
                     /gene="dcp"
                     /locus_tag="OO172_003950"
     CDS             3295..5340
                     /gene="dcp"
                     /locus_tag="OO172_003950"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5513914.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1337 a         1299 c         1413 g         1354 t
ORIGIN      
        1 gatgcgtgga cggctcgcgt cgttggcgtc gttaaaaaag ccttgcacgt acagatggat
       61 ggcttagaaa ccgtgttggc tgcgatgtgt gaaccgcaaa tcgcgattgt ctctctgaca
      121 atcaccgaaa aagggtattt ccactctccg gcgaccggac agttaatgct cgatcacccg
      181 atggtcgctg ccgacgtgca aaatccccac cagccgaaaa cagcaacatg ggtgattgtt
      241 gaggcgctgg ctcgccgtaa agcggcagga cttcccgcat tcccgcattt accgtcatgt
      301 catgtgacaa catgccagaa aacggtcatg ttatgcgtga cgttgtcact tcctacgcgc
      361 aagccgttga tgtaaaactg gcacaatgga tcgaagataa cgtgactttc ccatcaacaa
      421 tggtggaccg tattgtgccc gcagtggcag aggatacgct ggcgaaaatc gaacaactta
      481 ccggtgtgcg cgatcctgct ggcgttgcct gtgaaccttt ccgccagtgg gttatcgaag
      541 ataactttgt agccggacgt ccggaatggg aaaaagcggg agccgaactg gttagtgatg
      601 tgctgcctta tgaagagatg aagttgcgca tgctcaacgg cagtcattca ttcctggcat
      661 atctgggtta tcttgctgga tatcagcaca ttaatgactg tatggaagat gaacattatc
      721 gtcatgcggc gtatggcatg atgttgcagg aacaagcgcc gacgctgaaa gtgcagggcg
      781 ttgatttgca agattacgct aaccgattaa ttgcacgcta tagcaacccg gcgttacgtc
      841 atcgaacctg gcagattgcg atggatggca gccagaaatt gccacagcgg atgttggatt
      901 ctgttcgctg gcatctggcg catgacagca agttcgatct gctggcgctg ggcgtcgcgg
      961 gttggatgcg ttatgtcggt ggtgttgatg aacagggaaa tccgatagaa atcagtgacc
     1021 cactgttacc tgttattcag aaggctgtac aaagtagtgc cgaagggaaa gcgcgcgtcc
     1081 agtcattgct ggcgattaag gcaatctttg gtgatgattt gccagacaat agcttgttta
     1141 ctgcaaaagt gacggaagcg tacttgtctt tattagcgca tggcgcgaaa gcgaccgtgg
     1201 cgaaatattc cgtgaagtaa aaaaagcaat gccgccatcg gcggcattca cacacttagt
     1261 tcatgccgag acgaatcagg tccagaggtg ccagtttctc ttcacaacct tcaacaacta
     1321 cggtttttcc gcggcggatt tgctcagcgg tcagaccttc agtatcaatc gacaggatct
     1381 tgccagtgtg accggtgccg ctaaccataa cacggctgcc agtggtgatt gcgttacggt
     1441 tacgatcgta ggtcgtcata ggtgttttct cctttctgat ttacagtcga agcccacgta
     1501 acgggcgcaa tataaatact cctgtcgagt gaaatatttt ttgtttttga tcaagctcac
     1561 atcttttttg atgcatgcat gagccgtaag gcggggggaa aagcctctca tcccggatgg
     1621 gatgagagta aatgaattac tcttcgctaa accagttgct gttttcctgg cggatctggc
     1681 gcacggattc gctgatttcc tgcaaatgtt gcgtcattgc acgttctacc gcatcgccat
     1741 cacgtttttg cagggcagag aaaatatcaa gatgctggcg taacagcatt tctggtggag
     1801 aaacatggtc gaaactcata tagcgcacgc gatcaacggt cgctttcagg ttctcaatgg
     1861 tatcccacgc cagttgacag tcggcaatct gcgtcaggag ttgatggaag ttgtcatcaa
     1921 gttcaaaaaa atcatccagt tgcttgcgct caatggcaat gcgttgctgg tgaagatttt
     1981 gttccagttg gtagcactgg ctttcggtaa tcatgctcgc cgcccgacgc gccaccgcgc
     2041 actcaatggc ctgacggata aaactgccgt tgcgcacctg ggccatggaa attttgttga
     2101 cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt ttccgccagt ttaataaagg
     2161 cttcacgaac cggctggcgt gacacattga aacgaacaga aacttctttt tccgacaacg
     2221 gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg aagaatacga taaatctgct
     2281 gattgacagg ctgtgtggga ttaagttgcg tttcgacggt cattcgttct tgcttgtgag
     2341 tgagttaact gcatgagtct accacttttt tgtgacaagc aataacccgg cagttacgcc
     2401 gggtataaaa attactgacg gtggacattc agtccggcat agctttgggt aaccggcatc
     2461 atttccaggg tattgatatt gacgtgagca ggcagcgttg acacccacca gacggcttcg
     2521 ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg ttttttccgc tttaccgtca
     2581 tcgcctttaa agcggacatt ggaaaactcg gtgccaccca ccagacccgg ttcgatgtcg
     2641 gtgacgcgca ccgccgtacc atgcagatcc gtacgcagat tcaggctaaa ctgacgaaca
     2701 aacgctttcg tcgcaccgta aacgttacca ccggcatacg gccagctgcc tgccgttgag
     2761 ccaatgttaa taatatgacc atgattacgt tcaaccatac ccggtaagac ggcgcgcgtc
     2821 atatatacta ggcctttgtt gttggtatca atcatcgttt cccagtcttc aacgctggct
     2881 ttatgcgcag gctccatgcc caacgccagg ccggcattat ttaccaggat atcaatattg
     2941 ctccactcgg cagggagcga tgccagcatc tcttcaatag cggcgcggtt gcgaacgtcc
     3001 agttgggcga tatacagatt atctcccagt tcgtctttta actcctgcaa ccgctcctgg
     3061 cgacggccag tggcgataac tttatgccct tgttgaataa aacgacgagt aatgcattca
     3121 ccaaaacctg ccgttgctcc agttactaaa acgatcatct cactgttcct caacgctttt
     3181 gtgtgtcaat aacatagcac gtgatgctta agagcgggta atactggttt gcaagattgc
     3241 actccgttat cagtaagcct acgctatgga acacctcgcg ttcaggagaa gaaaatgaca
     3301 acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc tggctcctca ttttgatcaa
     3361 attgccaatc atcactatcg cccggcattc gatgagggaa tgcaacaaaa gcgggcagaa
     3421 attgctgcca tcgcgcttaa cccgcaaacg cctgatttca acaatactat tctggcactg
     3481 gaacaaagcg gagaattaat tacccgcgtt accagcgtct tttttgcgat gactgcggcg
     3541 cataccaatg atgaattaca gcgtcttgat gaacagtttt ccgctgaact ggcggaactg
     3601 gctaatgata tctatctgaa cggtgaatta ttcgcgcggg tagatgctgt ctggcagcgc
     3661 cgtgaatccc tggggcttga tagtgaatcc atccgcctgg tggaggttat tcatcaacgt
     3721 tttgtccttg ccggagccaa acttgcacaa gctgataaag caaaattaaa agtactgaat
     3781 acagaagctg cgaccctgac cagccagttt aaccagcggt tacttgcagc aaataaatcc
     3841 ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa tgagtgagca agagattgcg
     3901 ctggcggcag aggcggctcg cgagaaaggt ctggataaca aatggctgat tccgctgctg
     3961 aataccaccc aacaaccggc gcttgccgaa atgcgcgatc gtgcgacgcg tgaaaaactg
     4021 tttattgcgg gctggacgcg agcggaaaaa aatgatgcca atgatacccg cgctatcatt
     4081 caacgtctgg tagagattcg cgcacagcag gcgaaactgc ttggttttcc tcattatgcc
     4141 gcatggaaaa tcgccgatca gatggcaaaa acgccagaag cagcactcaa ctttatgcgg
     4201 gaaattgttc cagcggcgcg tcaacgtgcg agcgatgaat tagcctccat acaggcggtt
     4261 atcgataagc agcagggcgg gtttagcgcg cagccgtggg actgggcatt ttatgcggaa
     4321 caggtacggc gggagaaatt tgatcttgat gaggcgcagc tcaagccata ttttgaatta
     4381 aacacggtgt taaatgaagg tgtattctgg accgcgaatc agctcttcgg tattaagttt
     4441 gtcgaacgtt ttgatattcc ggtctaccat cctgacgttc gtgtgtggga aatttttgat
     4501 cataatggcg tgggactggc gttattttac ggtgatttct tcgcccgtga ttcaaaaagc
     4561 ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc ttaatgaaac gcatccggta
     4621 atttataacg tctgcaatta tcagaaaccc gctgccggtg agcctgcgtt gttactctgg
     4681 gatgatgtaa taaccttatt ccatgaattt ggtcatacgc tgcacggcct ttttgcccgc
     4741 cagcgttatg ccacgctttc cggcaccaac acgccgcgtg attttgtcga atttccgtcg
     4801 caaatcaacg aacactgggc aacgcatccg caggtattcg ctcgctacgc ccggcattat
     4861 cagagcgggg cagcaatgcc tgacgaactg caacagaaaa tgcgtaatgc cagcctgttc
     4921 aacaaagggt atgagatgag cgaactgctt agcgccgcac ttctcgatat gcgctggcat
     4981 tgcctggaag aaaacgaagc aatgcaggat gtcgatgatt ttgaattgcg ggcgctggtg
     5041 gcggaaaata tggatcttcc tgctataccg ccacgctatc gcagcagtta tttcgcccat
     5101 atttttggtg gcggatatgc tgcaggttat tacgcttatc tgtggacgca aatgttggcc
     5161 gatgatggtt accagtggtt tgttgagcag ggcggattaa cgcgtgaaaa tgggcagcgt
     5221 tttcgcgagg cgatcctttc cagaggtaac agcgaagatc tggaacgcct gcatcgacaa
     5281 tggcgcggta aggctcctca gattatgccg atgctgcaac atcgtggctt gaacatataa
     5341 acaccaccaa tggctggcct ggagatatcg ccaggccagc ctggttatag cagtaattgc
     5401 agc
//
LOCUS       DAJTNM010000195         5337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTNM010000195 DAJTNM010000000
VERSION     DAJTNM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.195"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO172_003951"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO172_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5513915.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO172_003952"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO172_003952"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5513916.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO172_003953"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO172_003953"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5513917.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO172_003954"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO172_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5513918.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1289 c         1456 g         1283 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaactc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgactttc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaaattaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DAJTNM010000196         5318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTNM010000196 DAJTNM010000000
VERSION     DAJTNM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.196"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..607
                     /locus_tag="OO172_003955"
                     /pseudo
     CDS             1..607
                     /locus_tag="OO172_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            671..1597
                     /gene="glsB"
                     /locus_tag="OO172_003956"
     CDS             671..1597
                     /gene="glsB"
                     /locus_tag="OO172_003956"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5513919.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1564..1956
                     /locus_tag="OO172_003957"
     CDS             1564..1956
                     /locus_tag="OO172_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5513920.1"
                     /translation="MDETVRAFGLLRQSLDPLFARLSRSKFRSRFRLGMKERQYCLEK
                     GAPVIEQHAADFVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIP
                     QGVSLSEEQQRYIVAVIYHWLVVQMNQP"
     gene            2095..3513
                     /locus_tag="OO172_003958"
     CDS             2095..3513
                     /locus_tag="OO172_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5513921.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3740..5191
                     /gene="uxaB"
                     /locus_tag="OO172_003959"
     CDS             3740..5191
                     /gene="uxaB"
                     /locus_tag="OO172_003959"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5513922.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1228 a         1238 c         1433 g         1419 t
ORIGIN      
        1 cccatcattc ttgcaggtaa cggctactta cttaaacatg cgccgaatgt gatgggctgt
       61 gctcagctca ttgcccaggt gtttaaagat gcgggaatcc cgcaaggcgt atatggctgg
      121 ctgaatgccg acaacgacgg tgtcagtcag atgattaaag actcgcgcat tgctgctgtc
      181 acggtgaccg gaagtgttcg tgctggagcg gctattggcg cacaggctgg agcggcactg
      241 aaaaaatgcg tactactatc cgccaacggt tctggcgaat gttaccccag aaatgaccgc
      301 gtttcgggaa gaaatgtttg gccccgttgc tgcaatcacc attgcgaaag atgcagaaca
      361 tgcgctggaa ctggctaatg atagtgagtt cggcctttca gcgaccattt ttaccaccga
      421 cgaaacacag gccagacaga tggcggcacg tctggaatgc ggtggggtgt ttatcaatgg
      481 ttattgtgcc agcgacgcgt gagtggcctt tggtggcgtg aaaaagagtg gctttggtcg
      541 tgagctttcc catttcggct tacacgaatt ctgtaatatc cagacagtgt ggaaagaccg
      601 gatctgacgc gacatattca gctctgatat actcgcaggt cttttcagac ctgcggtcca
      661 ggagtagaaa gtggcagtcg ccatggataa tgcaatttta gaaaacatct tgcggcaagt
      721 gcggccgctc attggtcagg gtaaagtcgc ggattatatt ccggcgctgg ctacagtaga
      781 cggttcccga ttggggattg ctatctgtac cgttgacgga cagctttttc aggccggaga
      841 cgcgcaagaa cgtttttcca ttcagtctat ttccaaagtg ctgagtctcg ttgtcgccat
      901 gcgtcattac tccgaagagg aaatctggca acgcgtcggc aaagatccgt ctggatcacc
      961 gttcaattct ttagtgcaac tggaaatgga acagggtatt ccgcgtaatc cgttcattaa
     1021 tgccggtgcg ctggtggtct gcgatatgtt gcaagggcga ctaagcgcac cacggcaacg
     1081 tatgctggaa gtcgtgcgcg gcttaagcgg tgtgtctgat atttcatacg atacggtggt
     1141 agcgcgttcc gaatttgaac attccgcgcg aaatgcggct atcgcttggc tgatgaagtc
     1201 gtttggcaat ttccatcatg acgtgacaac cgttctgcaa aactactttc attactgcgc
     1261 tctgaaaatg agctgtgtag agctggcccg gacgtttgtc tttctggcta atcaggggaa
     1321 agctattcat attgatgaac ctgtggtgac gccaatgcag gcgcggcaaa ttaacgcgct
     1381 gatggcgacc agtggtatgt accagaacgc gggggagttt gcctggcggg tgggactacc
     1441 ggcgaaatct ggcgttggtg gcggtattgt ggcgattgtt ccgcatgaaa tggccatcgc
     1501 agtctggagt ccggaactgg atgatgcagg taactcgctt gcgggtatcg ccgtccttga
     1561 acaattgacg aaacagttag ggcgttcggt ttattaaggc agtctctcga tccactcttc
     1621 gcgcgtttat cccgttcaaa atttcgctct cgctttcgtc tgggcatgaa agagcgtcag
     1681 tattgcctgg agaaaggcgc gccagtcatc gaacaacatg cggcggattt tgtcgctaaa
     1741 cgtcttgctc cggcattacc ggctaatgac ggtaagcaac cccccatgcg cgggcatccg
     1801 gtgtttatcg ctcagcacgc caccgcgacc tgctgtcgcg gttgtctggc taagtggcat
     1861 aacattccgc agggtgtttc gttaagcgaa gaacaacaac gttatatcgt ggcggtaatc
     1921 tatcactggc tggttgtaca gatgaatcag ccatgagaat tgcggaaaac gataaaatag
     1981 cattcccagg gtacctttat ttgccgccgc tgttagcgaa catgctatcc tttatgagaa
     2041 tttacttatc acgttgagat aattgccacc cgcaattgta tggacagata attaatgcat
     2101 gtacacccca tttcaacttt ccgtttgttc caggaaggtc atctgctacg taatagcatc
     2161 gctatttttg cgctaaccac gctgttctat tttattggtg cagagttacg gctggttcac
     2221 gaactttctc ttttctggcc gctgaatggc gtaatggcgg gggtgtttgc ccgctatgtc
     2281 tggcttaatc gactgcatta ctatgcgatc agttatgtgg cgatgctggt ttatgatgcc
     2341 ataaccaccg aatggggact ggtttcactg gttatcaatt tctccaatat gatgtttatt
     2401 gttaccgtcg ccttactggt cgtgcgggat aagcgtctgg ggaaaaataa gtatgagcca
     2461 gtaagtgcct tacggctatt taattactgt ctgattgccg cattattatg cgctattgtc
     2521 ggggcgattg gttcggtcag tattgatagt ctggatttct ggcctttgct tgcggactgg
     2581 ttcagtgagc aattctcaac gggcgtgttg atcgtgcctt gtatgctgac gttggctatt
     2641 cctggagtgc tgccgcgctt taaagcagaa cagatgatgc ccgccatcgc gcttattgtg
     2701 tcggttattg cctcggtagt cattggcgga gcggggagtc tggcgttccc gctgcctgca
     2761 ttaatctggt gtgcagtgcg ctatacgccg caggtaacat gtctgttgac ctttgtcacc
     2821 ggtgcggtgg aaatcgtact ggtggcaaat tcggtgattg atatctcggt cggttcgccg
     2881 ttctccattc cggaaatgtt ctccgcacgt ctcggtattg ccacgatggc gatatgccca
     2941 attatggttt cttttagcgt ggcagcgatc aatttgctaa tgaagcaagt tgcgctgcga
     3001 gccgactttg attttctgac tcaggtttac tcacggtccg gtctttatga ggcgctgaaa
     3061 agtccatcgc tgaaacagac acaacatctg acggtcatgc tgcttgatat cgactatttc
     3121 aaaagcatta acgataacta tggacatgaa tgtggcgata aagtgttaag cgtgtttgcc
     3181 cggcatattc agaagattgt cggtgataag gggctggtgg cgcgcatggg cggcgaagaa
     3241 tttgctgttg cagtgccgtc ggtgaatcct gtcgatggtc tgctaatggc ggaaaaaatc
     3301 cgtaaaggcg ttgaactgca accattcacc tggcaacaaa aaacgcttta tctcacggta
     3361 agtattggcg tcggtagtgg tcgcgcatcg taccgaacgc tgaccgatga ttttaataaa
     3421 ttgatggtcg aagccgatac gtgtctgtat cgctcgaaga aagatgggcg caaccgtacc
     3481 agcactatgc gttacggtga ggaagttgtc tgacatcttt attcattccg cgcgatttga
     3541 atgcaatgtg ttctaccact ttatgatcca ggaaaaggcg tatttcctcg attaaaccat
     3601 gatccgcgcc acacttttct cataaaactg ctcattaatc atcaggagtt gatcaaaaca
     3661 ggggttcggc gtattaaagt ttgctaacct gtcgcaccaa ttcaaacagg ttgtatgact
     3721 aatcagaagg gaacccattg tgaaaacact aaatcgtcgc gattttcccg gtgcacagta
     3781 tccagaacgt atcattcagt ttggtgaagg taacttcctg cgcgcctttg ttgactggca
     3841 aatcgatctc ctgaatgagc acaccgatct gaattctggc gtggtcgttg ttcgtccgat
     3901 tgaaacttca ttcccgccgt cactgagcac gcaggatggt ctgtacacca ccattatccg
     3961 tggcctgaat gagaaggggg aagcggtcag cgacgcgcgt ctgattcgct cggttaatcg
     4021 tgaaatcagc gtctacagtg aatacgatga attcctgaaa ctggcgcaca acccggaaat
     4081 gcgctttgtt ttctctaaca ccaccgaagc gggtatcagc taccatgcgg gcgataaatt
     4141 cgatgacgcg ccagcggtaa gctatccggc aaaactgacc cgtctgctgt tcgaacgctt
     4201 tagccatttc aacggtgcgc tggataaagg ttggatcatc attccgtgtg agttgattga
     4261 ctacaatggc gacgcgttgc gtgaactggt gctgcgctat gcgcaagagt gggcactgcc
     4321 agaagcattt attcaatggc tggatcaggc taacagcttc tgttctacgc tggtggaccg
     4381 tatcgttacc ggttatccgc gcgatgaagt ggctaaactg gaagaagaac tgggttatca
     4441 cgatggtttc ctcgacaccg ctgaacattt ttacctgttt gtgattcagg ggccgaaatc
     4501 cttagcgact gagctgcgcc tggacaaata tccgctcaac gtgctgattg ttgacgatat
     4561 taaaccgtat aaagaacgca aagtggcaat cctcaacggt gcgcacaccg cgctggtgcc
     4621 ggtggcgttc caggcagggc tggataccgt aggtgaagcg atgaacgatg ctgaaatttg
     4681 cgcattcgtt gaaaaagcca tctacgaaga aattattccg gtactggatt tgcctcgtga
     4741 tgaactggaa tctttcgcca gtgcagtcac cgggcgtttc cgtaacccgt acattaagca
     4801 tcagctgctg tctatcgcgc tcaacggtat gaccaagttc cgcacccgca tcctgccgca
     4861 gctgctggca gggcagaagg caaacggcac acttccggcg cgccttactt tcgcattagc
     4921 ggcattgatt gcgttctatc gtggtgagcg taacggagaa acatatccgg tacaggacga
     4981 tgcacactgg ctggaacgtt atcagcaact ctggagccag catcgtgacc gtgttatcgg
     5041 tacccaggaa ctggtagcga ttgtgttggc agaaaaagac cactgggagc aggacctgac
     5101 gcaagtgcct ggtctggtcg agcaggttgc taatgacctg gatgcgattc ttgaaaaagg
     5161 gatgcgcgaa gctgtacgtc cgttgtgcta ataacaccgt tacttctgaa cccggtttcg
     5221 accgggtttc ttatttcact atattagtta caacatccta tatgccttta tcattaacag
     5281 gtgtcacatg cagcgcctgt taatgttgta agcttgac
//
LOCUS       DAJTNM010000197         5243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTNM010000197 DAJTNM010000000
VERSION     DAJTNM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.197"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..542
                     /locus_tag="OO172_003960"
     CDS             <1..542
                     /locus_tag="OO172_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5513923.1"
                     /translation="LPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGSGNPRNFT
                     CNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTD
                     SSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTG
                     NKVSAGTVQSVIGVTFIYE"
     gene            876..3155
                     /gene="ydeP"
                     /locus_tag="OO172_003961"
     CDS             876..3155
                     /gene="ydeP"
                     /locus_tag="OO172_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5513924.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3403..3600
                     /gene="safA"
                     /locus_tag="OO172_003962"
     CDS             3403..3600
                     /gene="safA"
                     /locus_tag="OO172_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5513925.1"
                     /translation="MHATTVKNKIMQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3674..4435
                     /gene="ydeO"
                     /locus_tag="OO172_003963"
     CDS             3674..4435
                     /gene="ydeO"
                     /locus_tag="OO172_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5513926.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4837..>5243
                     /locus_tag="OO172_003964"
     CDS             4837..>5243
                     /locus_tag="OO172_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR5513927.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSR"
BASE COUNT         1512 a         1117 c         1160 g         1454 t
ORIGIN      
        1 cattaccgtt aaaactctat attaccccag ttggggcggc cggtggagtt gtcataaaag
       61 cgggggaagt cattgcccgt attcatatgt ataaaattgc aacattagga agcggaaatc
      121 cgcgtaattt tacgtgtaat attatttcaa ataacagtgt tgttatgcca acaggcggct
      181 gcaccgttga tagtcgtaat gtcaccgtgg atctcccgga ctttcctgga agcgcagaaa
      241 taccccttgg cgtatattgc tcaagcgaac aaaagctctc attttatctt tcgggtgcca
      301 ccactgacag tagccgacaa gtgtttgcta acacagcacc tgatgcaaca aaagcatcgg
      361 gagtgggtgt cactctaatg cgtaatggaa aaatactggc tacgggagaa aatgtctccc
      421 tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag
      481 gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat
      541 aattgtgatt ttgaccttag tctatgcgct ccattcacga aaattgcgcg gataattatt
      601 ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa gccagagtct
      661 tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg cttaacttat
      721 cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt agtcccctac
      781 tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat cgctattaca
      841 aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt gaatcctacc
      901 agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga
      961 tggatatacg ccatgatgtt attgccatgt ttgacatgaa taagccagag ggctttgact
     1021 gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg
     1081 gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct ttctttgctg
     1141 agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct gcggggcgtc
     1201 tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta agctggcaac
     1261 aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat caggttgaat
     1321 tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt tttgcccgtg
     1381 aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg acaagcgtgg
     1441 gtttggcagc gagtatcggt gtaggtaaag ggaccgtgtt gctggaagac tttgagaagt
     1501 gcgatttagt catttgcatt gggcataacc ctggtacaaa ccaccctcgc atgctgactt
     1561 cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct ctacaggaac
     1621 gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg aactctgaga
     1681 ctcagttggc cagtgcctac tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg
     1741 ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt cggccctcat
     1801 tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta cgccgtgacg
     1861 ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag acacaaatcg
     1921 ccgaactggc tgacgcatat gccgctgccg aacgcaccat tatctgttac ggaatgggga
     1981 tcactcagca cgaacatggt acccagaacg tgcagcaact ggtcaatttg ctgttgatga
     2041 aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgtggtcac tctaatgtac
     2101 agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg gctcgcctgg
     2161 gtgagcgcta tggcttcccc ccacctcatg cacctggaca tgctgcaatt gccagcatgc
     2221 aagcaatatg tacggggcag gctcgagcat tgatctgcat ggggggcaac tttgcactgg
     2281 caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg gcggtacacg
     2341 tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat attctgccgg
     2401 ttctggggcg tagcgagatt gatatgcaaa aaagcggtgc gcaggcggta accgttgagg
     2461 attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga
     2521 aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccag agcgtggtag
     2581 cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa gctgtgctgc
     2641 cagagttcgc cgactataac cagcgcatcc gtcatcccgg tggttttcac ctgataaatg
     2701 cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt accagcaaag
     2761 ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg acagtacgca
     2821 gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga ggggtattcg
     2881 gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg
     2941 gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg
     3001 atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc tattttccag
     3061 aatcgaatca catgctaaca cttgataacc acgatccatt aagtggcatt cctggctata
     3121 aaagtattcc ggttgaatta gaaccatcaa attaatgcct cttctcattt cttctgctgt
     3181 catccgcaca gcagaagaat tcctcattga ctattattgc gcaatttact cacatggatt
     3241 aaattaaact acatactata agatataaac ttctgcctac agctgtaaga aactccgctc
     3301 agtactgaag caccagtcct atttcttctt ttctccagcc tgttatatta agcatactga
     3361 ttaacgactt ttaacgttat ccgctaaata aacatatttg aaatgcatgc gaccacagtg
     3421 aaaaacaaaa tcatgcaaag agataactat aaagaaatca tgtctgcaat tgtggttgtc
     3481 ttattactga cacttacgtt gatagccatt ttttcggcaa ttgatcagct gagtatttca
     3541 gaaatgggtc gcattgcaag agatcttaca catttcatta tcaatagttt gcaagactga
     3601 aaataaacag caaattataa atgtgaaatg tttaaaaggt atcgataaaa ctttattgtt
     3661 ttaaggagat aaaatgtcgc tcgtttgttc tgttatattt attcatcatg ctttcaacgc
     3721 taacatttta gataaagatt acgccttctc tgacggcgag atcctgatgg tagataacgc
     3781 tgttcgtaca cattttgaac cttatgagcg gcattttaaa gaaatcggat ttaatgaaaa
     3841 taccattaaa aaatatctac aatgcactaa catccagaca gtgacggtgc ctgttcctgc
     3901 gaagttttta cgtgcttcaa atgtaccgac tggattgctt aatgaaatga ttgcttatct
     3961 caactcggaa gaacgcaatc atcataattt ttcagaactt ttgctttttt cttgcctgtc
     4021 tatttttgcc gcatgcaaag gtttcattac actattaact aacggtgtgc tatccgtttc
     4081 cgggaaagtg agaaatattg tcaacatgaa gctggcgcac ccatggaagc tgaaagatat
     4141 ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac
     4201 gacattctca cagattctat tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt
     4261 agaaggttca gtcaataaaa ttgccgaaca atgtggttat gccagtacat cttattttat
     4321 ttatgcgttc cgcaaacatt tcggcaacag tccgaagaga gtttctaagg agtaccgttg
     4381 tcaaaggcac acgggtatga atacgggcaa cacgatgagt gctttagcta tttgattatt
     4441 tgctaacgag tagtcaacca cgcacgctac gtaagaatta tatggggcag ccattccctg
     4501 ccccgcgttg tttttaggcg atatatttat tgaaataaat aagtgacatc catcacatat
     4561 ttatgcactt gcataacctg ttgcatgatt atttataatc tcaattcggc attttgtcag
     4621 taaaatgcaa taatttatta aatatcaata aattagttat ttatcggcga gaaattactt
     4681 aatagaacag aaagtaatgt caacgcttta tggactgtta tttccctttt tttagctaaa
     4741 tctgctatct ctttatgtga ctaacttcac ttacatccac ttatttctct tcgtaaaatt
     4801 acttcggaat taagtacaat aagaagagga acatttatga agtctgcatt aaagaaaagt
     4861 gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt tgctgctcat
     4921 gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc agactttacg
     4981 ccgactgaat acagtaccaa aggaaagcca aatattatcg tactaaccat ggatgatctt
     5041 ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat ggaaaatcgt
     5101 gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc acaaaaatca
     5161 acgccgacgc tcctttcatt aatggatgaa ggcgtacgtt ttactaacgg ctatgtggca
     5221 cacggtgttt ccggcccctc ccg
//
LOCUS       DAJTNM010000198         5137 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTNM010000198 DAJTNM010000000
VERSION     DAJTNM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.198"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..243
                     /locus_tag="OO172_003965"
     CDS             <1..243
                     /locus_tag="OO172_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5513928.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            430..687
                     /locus_tag="OO172_003966"
     CDS             430..687
                     /locus_tag="OO172_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5513929.1"
                     /translation="MTLPVFITVIADHDKPQPSGCLLESQGSLCPICRQRITHETGWN
                     VHHKVKKVMGGGEELSNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            830..1051
                     /locus_tag="OO172_003967"
                     /pseudo
     CDS             830..1051
                     /locus_tag="OO172_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1072..1134)
                     /locus_tag="OO172_003968"
                     /pseudo
     CDS             complement(1072..1134)
                     /locus_tag="OO172_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1584..2996
                     /gene="ospC1"
                     /locus_tag="OO172_003969"
     CDS             1584..2996
                     /gene="ospC1"
                     /locus_tag="OO172_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5513930.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(3100..3249)
                     /locus_tag="OO172_003970"
                     /pseudo
     CDS             complement(3100..3249)
                     /locus_tag="OO172_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3325..5022
                     /gene="ospD3"
                     /locus_tag="OO172_003971"
     CDS             3325..5022
                     /gene="ospD3"
                     /locus_tag="OO172_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5513931.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1761 a          940 c          988 g         1448 t
ORIGIN      
        1 gagctgaaaa ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca
       61 tcggatggac gtgcacagct tgatcgtgat gcgtactgca accgtaatgt cgttgaacgg
      121 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
      181 aattacctgg cgatggtgaa actgggctgc attcgactct tttatcaacg cttacgtaat
      241 taagggacac agcctagtct ccaggattcc cggggcggtt caatcaaagc gcagaacttc
      301 tgcgtcttct tcgtcaaaaa aatcatcttc gtgaagcttc acgacatagc ggggaagttt
      361 gcttctttga gaggcgggtt tacgtttacg gggtttagct gaacgggcca tataaccacc
      421 tgaaagacaa tgacattgcc tgtttttata acggtaattg cagaccatga caaaccgcag
      481 ccgtcaggct gcctactcga gtcacaaggc tctttgtgtc cgatatgccg gcagcgcatt
      541 acccatgaaa ctggctggaa cgtacaccat aaggtgaaga aagttatggg gggcggtgaa
      601 gaattatcta accttgtctt gttacatcca aattgccatc gacagttaca cagttgtgaa
      661 gccggctctc tcaaaaaggg cttataaagg cttgagcggt gtaccgggaa accggtatgc
      721 accgttctta ggggagggca gtccagcaat gaactgtcct tacccgacaa acatccaaaa
      781 cgaacggaac gtgctggaat ccgcatataa cccgttgtaa agtgaggaaa tggacacctc
      841 acttgctcat gagaacgccc gcctgcgggc actgttgcag acgcaacagg acactatcca
      901 ccagatggct gaatacaacc gcctgctctc acagcggatg gcggcttatg cttccgaaat
      961 caaccgactg aaggcgctgg ttgcgaaact gcagcgtatg cagttcggta aaagctcaga
     1021 aaaacttcgc gcaaaaaccg aacggcagat tccattttca cgggtgatct acgccacaca
     1081 ggctctgggt tgcagtgact gctccattaa ggcgattttg aactcataag ggaagtaaca
     1141 aaaaaactgc aaaagtacaa taattatata tggccaaact aacctctatt aaaaaacatc
     1201 agtttttcac acttagttga gcttcatgaa aaaatcatcg aggatttcat tgcagaatac
     1261 acattccatt aactggaagc actccataag aggaatcaga gaaagtatcg tttttttata
     1321 gtaaaattca ttgctgttca ataaaattga tatagttatt aaattatgaa aataatttca
     1381 caaaaaagat taacttgagt tcttaagcat cggattttag atattttgaa atatctggca
     1441 tcacataaca tcaaaagtat ataataccct tatctaatta catgcagtat attgtatata
     1501 tactcaaaaa ttgtaattca taccctgcta tatattgtag ggaaagatat taattaaaac
     1561 tgttttcata taaggttcat tttatgaata tatcagaaac actgaactca gcaaataccc
     1621 aatgcaatat agattctatg gataacagat tacatacatt gtttccaaaa gtgacatcag
     1681 tgcgaaacgc tgcacaacaa actatgccag atgaaaaaaa tttaaaagat agtgcaaata
     1741 ttattaaaga tttctttagg aaaactatag cagcacagag ttatagtaga atgttctctc
     1801 aaggctctaa ctttaaatct ttaaatatag caattgatgc accatcagac gctaaagcct
     1861 catttaaggc tattgagcac cttgacagat tatcgaagca ttatatatct gaaataaggg
     1921 aaaaacttca tcctctttct gcagaggaac tcaatttgct ttcgctaatt attaattctg
     1981 atttaatctt cagacatcaa agtaattctg atttgtctga taaaatttta aacattaagt
     2041 cattcaataa aattcagtct gaaggaatat gcacaaaacg aaacacatac gctgatgata
     2101 taaaaaaaat agctaatcat gactttgtgt tttttggcgt tgaaatctct aaccatcaga
     2161 aaaaacaccc cctgaataca aaacatcaca ctgttgattt tggtgcaaat gcgtatatca
     2221 ttgatcatga ctctccatat ggatatatga cattaaccga tcactttgat aatgctattc
     2281 cacctgtttt ttaccatgag caccaatcat ttttagataa attttcagag gttaataaag
     2341 aagttagtcg atacgtacat ggaagtaaag gaattataga tgtaccaata ttcaatacta
     2401 aagatatgaa gttagggctc ggattatacc tgattgactt tattagaaaa agtgaagacc
     2461 aaagcttcaa ggagttttgc tatggaaaaa atcttgcccc tgtggatctg gatagaatca
     2521 taaactttgt ttttcagcca gagtaccata tacctaggat ggtaagtaca gaaaacttca
     2581 aaaaagttaa gattagagaa atatccttag aggaggctgt tacagcatct aattacgaag
     2641 aaattaacaa gcaggtcact aacaaaaaaa ttgctctcca ggctcttttt ctttcgatta
     2701 ctaatcaaaa agaggatgtc gccttatata tattatctaa ttttgagata actagacaag
     2761 atgttatttc cataaagcat gagttgtatg atattgagta tctacttagc gctcataatt
     2821 caagctgtaa agtacttgag tattttatca ataagggatt ggttgatgta aacacaaagt
     2881 tcaaaaaaac taatagtggg gattgtatgt tggataacgc aataaaatat gagaatgcag
     2941 aaatgataaa actattattg aaatatggtg caacatctga caataaatat atttaatcaa
     3001 aattgaatat cgtttagcaa aaggcagtat ttgcacaaaa tatcgatata ttgtttattg
     3061 tcagtatggc tcaatgtgat aatagttgga aagtttgatg gggttcgccc cgttgtagcg
     3121 gtagtcgaac tggaaggtct tcaggcactg cttacagcga tagagcagcc cccctgaact
     3181 ggaatggccg ttccggatac ccccctgagt ttcagagtaa cggggacaaa ccacatcaat
     3241 ctttgccatc aatcatccaa agggcaaaga gtacaacaac actaagtctg cgtcacaacc
     3301 catcaatgaa aggaatatat acatatgcca tcagtaaatt taatcccatc aaggaaaata
     3361 tgtttgcaaa atatgataaa taaagacaac gtctctgttg agacaatcca gtctctattg
     3421 cactcaaaac aattgccata tttttctgac aagaggagtt ttttattaaa tctaaattgc
     3481 caagttaccg atcactctgg aagacttatt gtctgtcgac atttagcttc ctactggata
     3541 gcacagttta acaaaagtag tggtcacgtg gattatcatc actttgcttt tccggatgaa
     3601 attaaaaatt atgtttcagt gagtgaagaa gaaaaggcta ttaatgtgcc tgctattatt
     3661 tattttgttg aaaacggttc atggggagat attatttttt atattttcaa tgaaatgatt
     3721 tttcattccg aaaaaagcag agcactagaa ataagtacat caaatcacaa tatggcatta
     3781 ggcttgaaga ttaaagaaac taaaaatggg ggggattttg tcattcagct ttatgatccc
     3841 aaccatacag caactcattt acgagcagag tttaacaaat ttaacttagc taaaataaaa
     3901 aaactgactg tagataattt tcttgatgaa aaacatcaga aatgttatgg tcttatatcc
     3961 gacggtatgt ctatatttgt ggacagacat actccaacaa gcatgtcctc cataatcaga
     4021 tggcctaata atttacttca ccccaaagtt atttatcacg cgatgcgtat gggattgact
     4081 gagctaatcc aaaaagtaac aagagtcgta caactatctg acctttcaga caatacgtta
     4141 gaattacttt tggcagccaa aaatgacgat ggtttttcag gattgctttt agctttacaa
     4201 aatgggcatt cagatacaat cttagcatac ggagaactct tggaaacttc tggacttaac
     4261 cttgataaaa cggtagaact actaactgcg gaaggaatgg gaggacgaat atcgggttta
     4321 tcccaagcac ttcaaaatgg gcatgcagaa actatcaaaa catacggaag gcttctcaag
     4381 aagagagcaa taaatatcga atacaataag ctgaaaaatt tgctgaccgc ttattattat
     4441 gatgaagtac acagacagat acccggacta atgtttgctc ttcaaaatgg acatgcagat
     4501 gctatacgcg catacggtga gctcattctt agcccccctc tcctcaactc agaggatatt
     4561 gtaaatttgc tggcctcaag gagatatgac aatgttcccg gacttctgtt agcattgaat
     4621 aatggacagg ctgatgcaat cttagcttat ggtgatatct tgaatgaggc aaaacttaac
     4681 ttggataaaa aagcagagct gttagaagcg aaagattcta atggtttatc tggattgttt
     4741 gtagccttgc ataatggatg tgtagaaaca attattgctt atgggaaaat acttcacact
     4801 gcagacctta ctccacatca ggcatcaaaa ttactggcag cagaaggccc aaatggggta
     4861 tctggattaa ttatagcttt tcaaaatagg aattttgagg caataaaaac ttatatggga
     4921 ataataaaaa atgaaaatat tacacctgaa gaaatagcag aacacttgga caaaaaaaat
     4981 ggaagtgatt ttctagaaat tatgaagaat ataaaaagct gaatattatc tcgtggtgat
     5041 acactgaatt tgaggtaacc ggctcattta aaccgtctgg tctgttacct ccggttccac
     5101 aaaaataatg tccatcattt tgaagtggac actatcg
//
LOCUS       DAJTNM010000199         5086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTNM010000199 DAJTNM010000000
VERSION     DAJTNM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.199"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            157..1272
                     /gene="adrA"
                     /locus_tag="OO172_003972"
                     /gene_synonym="dgcC"
     CDS             157..1272
                     /gene="adrA"
                     /locus_tag="OO172_003972"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5513932.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1289..2098)
                     /gene="proC"
                     /locus_tag="OO172_003973"
     CDS             complement(1289..2098)
                     /gene="proC"
                     /locus_tag="OO172_003973"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5513933.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2218..2676
                     /locus_tag="OO172_003974"
     CDS             2218..2676
                     /locus_tag="OO172_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5513934.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2859..3383
                     /gene="aroL"
                     /locus_tag="OO172_003975"
     CDS             2859..3383
                     /gene="aroL"
                     /locus_tag="OO172_003975"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5513935.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3433..3624
                     /gene="yaiA"
                     /locus_tag="OO172_003976"
     CDS             3433..3624
                     /gene="yaiA"
                     /locus_tag="OO172_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5513936.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3882..4559
                     /locus_tag="OO172_003977"
     CDS             3882..4559
                     /locus_tag="OO172_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5513937.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLEKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4631..4915
                     /gene="ppnP"
                     /locus_tag="OO172_003978"
     CDS             4631..4915
                     /gene="ppnP"
                     /locus_tag="OO172_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5513938.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1233 a         1209 c         1337 g         1307 t
ORIGIN      
        1 agtcatcctg tttacctctt tctcaggaag tttagtctcc aggattcccg gggcggttca
       61 gatagtgcta acgggtgagc tacgaaagtg gctcacccga aatttcatac ttctgccttt
      121 agccccgtct ctataatttg ggaaaattgt ttctgagtgt tcccaaaaat aatgaatgat
      181 gaaaactttt tcaaaaaagc ggcggcgcat ggggaggaac ctcctttaac tcctcaaaac
      241 gaacatcagc ggtccgggct gcgcttcgcc cgtcgcgtca gactaccccg tgcggttggc
      301 ctggctggca tgttcttacc gattgcttca acgctggttt cgcacccgcc gccgggctgg
      361 tggtggctgg tgttggtcgg ctgggcgttc gtctggccgc atttagcctg gcagatagcg
      421 agcagggccg tcgatccgct tagccgggaa atttacaact taaaaaccga tgcagtatta
      481 gcgggaatgt gggtaggcgt aatgggcgta aacgtgctgc cttccaccgc tatgttgatg
      541 attctgtgtc tgaatttgat gggggcaggc ggcccccgtc tgtttgtcgc gggtctggtg
      601 ttgatggtgg tttcctgcct tgtcaccctc gagcttacgg gcattaccgt gtcgttcaat
      661 agtgcgccgc tggaatggtg gctctccctt cccattattg tcgtttatcc tctgctgttt
      721 ggctgggtca gctaccagac ggcaaccaaa ctggcggaac ataaacgcag gttgcaggtc
      781 atgagtactc gcgacggcat gacgggcgtg tataaccgac gtcattggga aactatgtta
      841 cgcaatgaat ttgataactg tcggcggcat aatcgcgatg caacgttact gattatcgat
      901 atcgaccatt tcaagagcat caacgatacc tggggtcatg atgtgggcga tgaagcgatt
      961 gtggcgctta cccgacagtt acaaattacc ctgcgcggta gcgatgtgat tggtcggttt
     1021 ggcggcgatg agtttgcagt aatcatgtcc ggtacgccag ctgagagcgc cattaccgcc
     1081 atgttacggg tgcgtgaagg gctaaacacg ttacgcttgc cgaatacgcc acaggtaact
     1141 ttacggatta gtgtgggggt tgcgccgctg aacccacaaa tgagtcacta tcgtgagtgg
     1201 ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag ccggacgtaa ccgcaccgaa
     1261 gtggcggcct gacgtccggc gaaagtcatc aggatttgct gagtttttct gatttttcca
     1321 tacacttcgt catcgcttcg atcactgcgg cacggaagcc tttctcttcc agtacgcgta
     1381 ccgcttcaat ggtggtacct cccggtgagc agaccatatc tttcagtgcc cccggatgtt
     1441 ctcccgtttc cagcaccatt tttgcggaac ccattaccgc ctgagcggca aatttatacg
     1501 cctggacgcg tggcatcccg cccagcacgg cggcgtcggc catcgcttcg ataaacataa
     1561 atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg gtggatcatc ggctcagcaa
     1621 ttacttccgc ttcgccaaag cagcggaaaa tattcagcac atcagcggta tcttctgggg
     1681 ttaccagcgc gtttggcgtt acggaggtca tcccggcatt aaccagcgcg ggagtgttcg
     1741 gcatggcgcg gataattttc cggtcatggc ccagcgcgcg ggcaagctgg tcgagcgtga
     1801 cacctgcagc aatagaaacg accagagagt ctttattcag gctggaggtg atttcgctaa
     1861 gcactttaat catgatgcca ggtttaacgg cagcaaaaat gatgtcggcg atttgcgcca
     1921 cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc atgcagggcg gcgactttat
     1981 ccggggaggg ggtgtatacc cagatttgcc ctggaagcac ctgaccgctg gcaattagac
     2041 cgccgagaat ggcttttccc atattgccgc agccaataaa accgattttc ttttccattg
     2101 cctcactcct gccgtgaaat tcattgtttt gataatcgct ggcagaagca taaacagaac
     2161 tatgccggaa ggcaaaagcg cgacacaata gaggattacc caacaaagga tgactttatg
     2221 acaatttggg tggatgccga cgcgtgtccc aatgtaatta aagagatttt gtatcgcgcg
     2281 gcggaacgta tgcagatgcc gctggtactg gtagcaaacc agagtttacg cgtgccgcca
     2341 tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg acgttgccga taacgaaatt
     2401 gtccggcagt gtgaagcggg cgatttggtg atcaccgcag atataccttt ggctgctgaa
     2461 gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg aacgttacac gccagcgacc
     2521 attcgtgagc gcctgacgat gcgcgatttt atggatacct tacgtgccag tgggatccag
     2581 accggcggac cagatagcct ttcacaacgt gaccgccagg cctttgccgc ggagctggag
     2641 aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt aatttattat ttacacttca
     2701 ttcttgaata tttattggta tagtaaggga tgtattgaga ttttcacttt aagtggaatt
     2761 ttttctttac aatcgaaatt gtactagttt gatggtatga tcgctattct catgacaccg
     2821 gctttcgccg cattgcgacc tattggggaa aacccacgat gacacaacct ctttttctga
     2881 tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc ccttgccgat tcgcttaacc
     2941 gtcggtttgt cgataccgat cagtggttgc aatcacagct caatatgacg gtcgcggaga
     3001 tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga aacggcggcg ctggaagcgg
     3061 taactgcgcc atccaccgtt atcgctacag gcggcggcat tattctgacg gaatttaatc
     3121 gtcacttcat gcaaaataac gggatcgtgg tttatttgtg tgcgccagta tcagtcctgg
     3181 ttaaccgact gcaagctgca ccggaagaag atttacggcc aaccttaacg ggaaaaccgc
     3241 tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc gctatatcgc gaagttgcgc
     3301 atattatcat cgacgcaaca aacgaaccca accaggtgat ttctgaaatt cgtagcgccc
     3361 tggcacagac gatcaattgt tgattttaga gcgcctatac ttaacgttca tcccgtgaaa
     3421 taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg aagcatatat agtgacgatt
     3481 gaaaaaggaa agccaggaca gacggtaacc tggtaccaac tcagagccga tcatcctaaa
     3541 ccagactcgt tgatcagtga acatccgacg gctcaggaag cgatggatgc gaaaaaacgc
     3601 tatgaggacc ctgacaaaga gtgaccgcat cagactgctc ggaagggatt ctgagtgcca
     3661 ctacaaggga tctgcgtcac atttttcata attcatgttt ttctaataat tagaatatta
     3721 aacaataaca ttccattact ggaatcattt ggaatcttta cattatgccg tgcacgtctg
     3781 ctgctacgct ttttgtcatt tgtagcacaa gtaagtgtca gcagtggtgc ttcacacttg
     3841 cccggtaatt aacgacgaaa gaaaagtaag gtggatgaac aatgagtgcg tcgttggcga
     3901 tcctgaccat cggcattgta cctatgcagg aggttttgcc gctcctgacg gaatacattg
     3961 acgaagataa tatttcccat catagcctgc tggagaagtt aagtcgtgaa gaagtgatgg
     4021 cggagtacgc gccagaagca ggcgaagaca ccattctcac attattaaat gacaaccagc
     4081 tggcccatgt ttcgcgtcgt aaagtggagc gagatctgca aggtgtggtt gaagtgctcg
     4141 ataatcaggg ttatgacgtc attttattaa tgagtacagc aaacattagt agtatgactg
     4201 cgcgtaatac gatctttctt gagccgtcgc gaatattgcc tccactggtt tcctctattg
     4261 ttgaagatca tcaggtgggg gttatcgttc cggttgagga gatgctgccc gttcaggcgc
     4321 aaaaatggca aattttgcag aaatcgccgg tattttcatt gggtaacccc attcatgatt
     4381 cagaacaaaa aatcattgat gctgggaaag aattactggc aaaaggggct gatgtcatca
     4441 tgctggattg tttaggattc caccaacgtc atcgcgattt actgcaaaaa cagctcgatg
     4501 ttcctgtctt gctctctaac gtattgattg cacggctggc tgcggaatta ctggtgtaat
     4561 tttgcgtgac agccagcgcc tctggcccct atagtgaagt agatgttcaa ctaccagaca
     4621 gggccagttt atgcttcaaa gtaatgagta cttttccggc aaagtgaaat cgatcggctt
     4681 ttccagcagc agcactggtc gcgccagcgt gggtgttatg gttgaaggcg aatacacctt
     4741 cagcaccgct gagccggaag agatgacggt aatcagtggc gcgctgaatg tgttactgcc
     4801 tgacgcgacc gactggcaag tgtatgaagc cggttcggtg tttaatgttc ccggtcacag
     4861 tgagtttcat cttcaagttg ccgaacctac ctcttatctg tgccgctatc tgtaattcct
     4921 cgccttcccc ttgaacggga gggcattttt ctgaaatatc ctctctttaa cccataataa
     4981 tatttccttt gctgcgattt tttcaatttc cgatatattc ataattcatc aaggttgata
     5041 taaatatcag tgaagatctc cagggtaatg actccaactt actgat
//
LOCUS       DAJTNM010000200         5077 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTNM010000200 DAJTNM010000000
VERSION     DAJTNM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.200"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(301..390)
                     /locus_tag="OO172_003979"
     CDS             complement(301..390)
                     /locus_tag="OO172_003979"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513939.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(826..987)
                     /locus_tag="OO172_003980"
     CDS             complement(826..987)
                     /locus_tag="OO172_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR5513940.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
     gene            complement(1639..1830)
                     /locus_tag="OO172_003981"
     CDS             complement(1639..1830)
                     /locus_tag="OO172_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5513941.1"
                     /translation="MTKQEKTALNMARFIRRQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            1879..2124
                     /locus_tag="OO172_003982"
     CDS             1879..2124
                     /locus_tag="OO172_003982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR5513942.1"
                     /translation="MKRERMLTIRVTDDEHVRLLERCEGKQLAVWMRRVCLGEPVARS
                     GKLPTLAPPLLRQLAAIGDELRRLRLAVREQGTRDDS"
     gene            2114..2710
                     /locus_tag="OO172_003983"
     CDS             2114..2710
                     /locus_tag="OO172_003983"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR5513943.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELTPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGR
                     LELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLP
                     DNLPETKQAIAEGVTRGLPCRRDKRPSGCDSGAHRGRD"
     gene            2791..3321
                     /locus_tag="OO172_003984"
     CDS             2791..3321
                     /locus_tag="OO172_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5513944.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELISN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWCQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            3328..3561
                     /locus_tag="OO172_003985"
     CDS             3328..3561
                     /locus_tag="OO172_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5513945.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(4142..>5077)
                     /locus_tag="OO172_003986"
     CDS             complement(4142..>5077)
                     /locus_tag="OO172_003986"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5513946.1"
                     /translation="ISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLLILL
                     SYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIGCVI
                     MLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLPEFM
                     VGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCISIII
                     YKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFILLMV
                     FFLTFYLSNKILIPTSKIESILIKKMNIINAKKAN"
BASE COUNT         1412 a         1061 c         1302 g         1302 t
ORIGIN      
        1 aaaaaaaaac aagctcacac ctacctggcc aagaacccct ccataaaatg atggtatgaa
       61 agatagcctg gtttggctaa atatgtagcc ataatgagat ataataacaa aaatgcaggc
      121 tgttactcgc aatgtatcaa ttgacggatc tctattagac ataaccttta tttcgctgtt
      181 caaattatcg cctttagttg atgatgtaaa attaacgcta taaataagct acacattcta
      241 gagcagcgaa aggatgattc aagtattttt aataataaaa cgcattgata atgtttatgt
      301 tcagatggtg ctgatagctt tgtcatgctg ttccactgat gttgataacg acagagactt
      361 ttgtcctttt cttgttagat gctgcctcag attcggatta tggcgttaaa ggggtttgac
      421 gctcaaccga acggtttcac acgttaagag ctttttgtct gataaatcaa aatataatgg
      481 acattgaaaa acgccctcaa aacggtgtct ttttaatcga agattggcac aaaacggaca
      541 ccaccgtcca ttagtcgatt ttttagacgg cttttctcgc ctaacgtgag ttttcgttcg
      601 gttgagcatc agaccccttt tatattgtag atttctattt ttagacagtt ggaacaggac
      661 aattaacaac agatctactt gagatcattt gcttgtcgtc gtagtctctt tcccagaaac
      721 gaaaaaaccg ccctgcaagg cggtttctcg aaggttctca gagcgaccaa ctctttgaac
      781 caaggtaact ggcttatgag gagcgcagta accaaatctg tcctttcagt ttagccttaa
      841 caggcgcata acttcaagac taactcctct aaatccgtta ccaatggcta ccgccactgg
      901 cgatttgtcg tgtctttccg ggttggactc aagttgatag ttaccggaac gggcgtggta
      961 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
     1021 actgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg acaccggtaa
     1081 accgaaaggc aggaacagga gagcgcacga gggagccacc agggggaaac gcctggtatc
     1141 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcggtttttg tgatgctcgt
     1201 caggggggcg gagcctatgg aaaaacgcca tcggcgcggc cttcttcctg tttctggtct
     1261 tttgctcaca tgttctttcc ggcgttatcc cctgattctg tggataacag catttcgttt
     1321 ttgagtgagt agataccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg
     1381 aggaagcgga aaagcgcctg gacgtgcatt ttctccttac gcatctgtgc ggcatttcac
     1441 acccggtatg gcgcactttt catacaatct gcactgatgc cgcatggtta agcccgtata
     1501 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc taaaacctgc
     1561 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct cacagacaag ctgtgaccgt
     1621 ctccgggagc tgcatatgtc agaggttttc accgtcatcc ccgaaacgtg cgaggcagct
     1681 gcggtaaagc tcatcggcgt ggtcgtgaag cgattcacag atatcagcct gttcatccgc
     1741 gtccagttcg ttgagcttct ccagcagcgt taatgtctgc cttctgataa agcgggccat
     1801 gttaagggcg gttttttcct gtttagtcac tgatacctcc gtgtaagggg gatttctgtt
     1861 catgaggtaa tgataccgat gaaacgcgag aggatgctca cgatacgggt tactgatgat
     1921 gaacatgtcc ggttactgga acgctgtgag ggtaaacagc tggcggtatg gatgcggcgg
     1981 gtctgcctgg gtgagccggt tgcccgttcc gggaaactgc cgacactggc accgccgtta
     2041 ctgcgtcagc tggctgccat cggggacgag ctgcgccggc tgcgtctggc cgtcagggaa
     2101 caggggacgc gggatgatag ttaagtttca tgcccgggga aaaggtggtg gcagtggtcc
     2161 ggttgattac ctgctgggaa gggagcgtaa ccgggaaggg gcaacggtgc ttcagggtaa
     2221 tccggaagaa gtccgggaac tcatcgatgc cacgccattt gcgaagaaat acacgtccgg
     2281 tgttctgtcg ttcgcggaga aggagctgac gccgggagga cgtgaaaaag tgatggcgag
     2341 ctttgagcgt gtactgatgc ccggtctcga aaaaaatcag tacagcatcc tgtgggtgga
     2401 gcaccaggac aagggacggc ttgagctgaa ttttgtcatt ccgaacatgg agctacagac
     2461 cggaaaacgc ctccagccgt actacgaccg cgcagacagg cctagaattg atgcctggca
     2521 gacgctggtt aatcaccatt acgggctgca tgacccgaac gccccggaga accgcaggac
     2581 gctgacactc cctgataatc tgccggaaac gaaacaggcg attgctgagg gcgttacacg
     2641 cggtttacca tgccggagag ataaaaggcc gtcaggatgt gattcaggcg ctcacagagg
     2701 cagggattga cgtggtcagg gtgacgcgaa gcagtatcag cattgcagac ccgaacggcg
     2761 ggaagaatgt cagactgaaa ggagcgtttt atgagcaatc ttttacagac gggtgcggag
     2821 ttcgagaaaa agctgaaaga gagagccgaa tctacagaga aaatgctgaa cgacgagttc
     2881 aggaagctcg aagaatctgt aaacagggaa ctgatctcaa acgaatcttt aatcaggaac
     2941 gctatcaacg accatacatc agccctgaaa gaacttctgg agaggtatca gaaaacgacc
     3001 gtcgatacga tggacgcaca ctggaaaacc gtactgaaaa tgagcgtgaa acgctggctg
     3061 tggctgatta tcgtctcagt actgatgttt gccacgaccg ggagcctgct gtggtgccag
     3121 ggcatgaaga taaacgcgaa catgaatatt ctccgcgagc agaaagagag tctggagaag
     3181 ctgaacgcga agacatgggg cgtgcgttat cacgaggaca gcaacggacg ttttctggtg
     3241 ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg aaaacgggaa acagaatgcc
     3301 gtaaggctgg tacaggagta acagagcatg acggagctgg aaagacattt gctgaacgca
     3361 ttagagcaac tgcaacagga ctatatgcag cggctgaacg aatgggagag cgccttcgtg
     3421 gaattgcaga aaatgttttc gcttacgcaa cgggacaacg cgatgctgaa cgagcgggtc
     3481 atgcagttga gtcagcaggt gcagcacttg agcgagcaga cagaacgctt gagccagtta
     3541 tacagcgaga acaggagata agggacgaac ggctgataca ggaatgcgaa catgcgttat
     3601 ccctggagcg tgagcgacag ccggaaatac aggaacggac actggatggc ccttcgctgg
     3661 gatggtaatc agagacaggc taaaatgttg tcagcgcgtt tccggtgcat tttttgggat
     3721 aatggttggt ttgggggggc accgggaacg cgcttcagtc agaccttgct gaaaacatag
     3781 gaacgcgtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgttgct gtgttttgcc
     3841 tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag gtccccgtct
     3901 tcttcgcttc cgaaagcatg aaaaaaatcc gtttcagctg tctctggctg gcaattcctg
     3961 cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt gcgatcgcgg
     4021 cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc gcgccgaacg
     4081 cgtttcagcg gtgcgtacaa tgagatgtta tggtaaattc aagtcttaga ttttatgatg
     4141 tttaattagc tttttttgcg tttataatgt tcatcttttt tattaaaatt gattcaatct
     4201 ttgatgtggg tattaatatt ttatttgaca aatagaaagt taaaaaaaac accattaata
     4261 gtataaataa atacccaaaa acagaagcgt tattaatttc aattattgat gttaatctgt
     4321 atattactac atgctgaatc aacatggata tgtagatgca tttagcatat gagttgaaaa
     4381 aatagaggtt aaatttctta ttaattatta atgctgcttt gtaaatgata atgcttatgc
     4441 acattaaagg tattattgat aactggtgat tgactatgtt tgttttgaac tcattaggcc
     4501 ctattaataa tactgtcaat atacatataa caaccgatag aataactgtg gtgttttttt
     4561 gtaatttatt tttgtattta aatgcaagca tcccaaccat aaactcagga agtcggcaag
     4621 caaaaaacca aaatgggaag tttaaaaatg atgatgagat atttgatatc gggaatgttg
     4681 taattgttat cacaaataaa aaaaatatgt tacgtttaaa tgtaaaggta ttcataacgt
     4741 gaaaaactat cggtgatatc acgtacagca taataacgca cccaataaac cactcgcctg
     4801 taaggaaaag aaaatcataa gagaaatggg cgtttaatat accatcaagg gcaaaaaata
     4861 ctggaattat tctagtgtac tggcctcctg ttgtcattat tgatacgaga gggactgtat
     4921 taattatctc tttatttaaa taagacaata aaattaagag taaagagata aaaaagtaag
     4981 tgataatata aggagtaagg atccttactc ctttgtcaag gaaataatca atggtattct
     5041 ttctatctag tgaaataaat gccaaatatc cagatat
//
LOCUS       DAJTNM010000201         5062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTNM010000201 DAJTNM010000000
VERSION     DAJTNM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.201"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..539
                     /locus_tag="OO172_003987"
     CDS             <1..539
                     /locus_tag="OO172_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5513947.1"
                     /translation="LIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNAMRKAVVGP
                     VVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVTQAQAEADS
                     KLAADKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVALTTAERWDG
                     KLPETMIPGSTVPFISTK"
     gene            complement(585..2576)
                     /gene="tkt"
                     /locus_tag="OO172_003988"
     CDS             complement(585..2576)
                     /gene="tkt"
                     /locus_tag="OO172_003988"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5513948.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            2854..3612
                     /gene="loiP"
                     /locus_tag="OO172_003989"
                     /gene_synonym="yggG"
     CDS             2854..3612
                     /gene="loiP"
                     /locus_tag="OO172_003989"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5513949.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(3818..4738)
                     /gene="speB"
                     /locus_tag="OO172_003990"
     CDS             complement(3818..4738)
                     /gene="speB"
                     /locus_tag="OO172_003990"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5513950.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4874..>5062)
                     /locus_tag="OO172_003991"
     CDS             complement(4874..>5062)
                     /locus_tag="OO172_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513951.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1203 a         1364 c         1383 g         1112 t
ORIGIN      
        1 gtctaattgt ccgtcagttg ccaactcagc tggagaacat tttcggccaa tataccgcca
       61 tcagcgccgt acaggatcgt acaaaattgg ttcaggatct gcaaaatgcc atgcgcaaag
      121 ccgttgttgg gccagtggta attgacgggg tccagattga aaatattgat ttttctgacg
      181 cttatgaaaa gtcgattgaa aaccgaatga aagcagaagt cgccattgcc acgcgtaagc
      241 aaaatctgga aacagagaaa atccaggcgc aaatagcggt aactcaggcc caggcagaag
      301 cagacagtaa actggcggca gacaaagcag aagcggaaac tattcgcgta agaggcgcgg
      361 cagaggctga aactattcgt ttaaaaagtg cggctgaagc agaagcaatc cgcctgcgcg
      421 gtgaagctct gcgggataat cccggacttg tagccctcac cactgccgaa cgttgggacg
      481 gtaaactgcc cgaaaccatg atccccggca gcacagttcc gtttatctct actaagtaaa
      541 aaaagggccg ctttcgcggc ccttctcaca aaacgtctta ctgattacag cagcgctttt
      601 gctttcgcaa caacgttatc aacagtgaag ccgaactctt caaacagcag ctctgccgga
      661 gcagattcac cgaaggtggt catgccgacg atagcgccgt tcaggccaac atacttgtac
      721 cagtagtcag caatacccgc ttctaccgca acgcgtgcag taaccgcttt cggcagtacg
      781 gattcacggt aagccgcatc ctgcttgtca aatgcgtcgg tagacggcat ggacaccacg
      841 cgcgctttca cgccttcggc agtcagtttt tcgtaggcag caaccgccag ttcaacttca
      901 gaaccggtgg cgatgaagat cagttccggc tgaccagcac agtctttcag cacataacca
      961 ccgcgcgcga tgtttgccag ctgctcttca gtacgttcct gctgcgccag gttctgacgg
     1021 gagaggataa gcgcagtcgg gccgtcctga cgctcaacgc cgtatttcca cgcgaccgcg
     1081 gattcaacct ggtcacacgg acgccatgta gacatgttcg gggtcacgcg cagagaagcg
     1141 acctgctcta ccggctggtg agtcgggcca tcttcgccca gaccgatgga gtcgtgggtg
     1201 taaaccatca cctggcgctg tttcatcagc gcagccatac gtacggcgtt acgtgcgtat
     1261 tccacgaaca tcaggaaggt ggaggtgtac ggcaggaagc caccgtgcag ggagataccg
     1321 ttagcaatcg cggtcatacc gaactcgcga acaccgtagt ggatgtagtt acctgcagca
     1381 tcttcgttga ttgctttaga accagaccac agggtcaggt tagacggtgc caggtcagca
     1441 gagccgccga ggaattcagg caacagcggg ccgaacgctt cgatagcatt ctgcgacgct
     1501 ttacggctgg cgattttcgc cggattagcc tgcagtttag cgataaactc tttcgctttg
     1561 gcgtcgaagt cagacggcat ttcgcctttc atacggcggg taaattcagc cgcttcctgc
     1621 ggataagctt tcgcgtaagc cgcaaacttc tcattccatg cagattcttt cgcctggcct
     1681 gcttctttcg catcccactg agcatagatt tcagacggga tttcgaacgg tgcatatttc
     1741 cagcccagct gttcgcgggt cagggcaatt tcagcgtcgc ccagcggcgc accgtgggag
     1801 tcgtgagtac cggctttgtt cggggaaccg aaaccgatga tggttttgca catcagcagg
     1861 gacggtttgt cagtcactgc gcgcgcttct tcaactgcgc gtttgatgga tgccgcgtca
     1921 tgaccgtcga tgtcgcgaat aacgtgccag ccgtaagctt cgaaacgcat tgctgtgtcg
     1981 tcggtgaacc agccttcaac gtgaccatcg atagaaatac cgttgtcatc gtagaacgca
     2041 atcagtttac ccagcttcag cgtacccgcc agagagcaaa cttcgtggga gatgccttcc
     2101 atcatgcagc cgtcgcccat gaaggcgtag gtgtagtggt cgacaatgtc gtggcccgga
     2161 cggttaaact gcgccgccag cgttttttct gcaatcgcca taccgactgc gttggcaata
     2221 ccctgaccca gcggaccggt ggtggtttcc acaccagcgg tataacctac ttccgggtgg
     2281 cccggagttt tagagtgcag ctgacggaag tttttcagtt cttccatcgg cagatcgtaa
     2341 ccggtgaggt gcagcaggct gtagatcagc atggagccgt ggccgttgga cagcacgaag
     2401 cggtcacggt cagcccagga cggattctgc gggttgtgtt tcaggaaatc acgccacagg
     2461 acttcggcaa tgtcagccat acccataggg gcacccgggt gaccggattt ggctttctgt
     2521 actgcgtcca tgctcagcgc acgaatagca ttggcaagct ctttacgtga ggacattttg
     2581 actccagatc ggatgatgaa gggcacgccc ttaacgactt gacgacagcg cgttttgggc
     2641 tacgccggaa aatttgccaa caatttaccg caagccgcgc gtcatgtaca tggaacatcc
     2701 ttttgccgct tcagaaatct ctggatcatg ctcgcatgtt gcgcaatctg ctcgcccgtc
     2761 cgctgcgctt ttccttatac tgagactgag cgtcgattca cctgcaaacg gcgcattttt
     2821 agaataatcc tgaccttgtg cggaagagaa aacatgaaaa ttcgcgcctt attggtagca
     2881 atgagcgtgg caacggtact gaccggttgc cagaatatgg actccaacgg actgctctca
     2941 tcaggagcgg aagcttttca ggcttacagt ttgagtgatg cgcaggtgaa aaccctgagc
     3001 gatcaggcat gtcaggagat ggacagcaag gcgacgattg cgccagccaa tagcgaatac
     3061 gctaaacgtc tgacaactat tgccaatgcg ctaggcaaca atatcaacgg tcagccggtg
     3121 aattacaaag tgtatatggc gaaggatgtg aacgcctttg caatggctaa cggctgtatc
     3181 cgcgtctata gcgggctgat ggatatgatg acagataacg aagtcgaagc ggtgatcggt
     3241 cacgagatgg ggcacgtggc gttaggccat gtgaagaaag ggatgcaggt ggcgcttggt
     3301 acaaatgccg tgcgagtagc tgcggcctct gcgggcggga ttgtcggcag tttatctcaa
     3361 tcacaacttg gtgatctggg cgagaaatta gtcaattcgc aattctccca gcgccaggaa
     3421 gcagaagccg atgattattc ttacgatctt ctgcaccaac gcggcatcag cccggcaggt
     3481 cttgccacca gctttgaaaa actggcgaag ctggaagaag gccgtcaaag ctcaatgttt
     3541 gacgaccatc ctgcatccgc cgaacgtgcc cagcatattc gcgatcgcat cagcgcggat
     3601 gggattaagt aaagcctggt ggtgtcgaac gtaggtcaga taaggcgttc acgccgcatc
     3661 tgacattaat ggcacatttg ttaccttgtg cgcattgccg gatgcgatgc tggcgcatct
     3721 tatccggcct acgggtgccg aacgtaggtc ggataaggcg ttcacgccgc atccgacatt
     3781 aatggcacgt tttacccgtg cgcatcgcat ctggtgctta ctcgcccttt ttcgccgcct
     3841 gaatatacag catctccagc gccagcgtcg ccgcagccag agcggtgatt tccgactgat
     3901 cgtatgccgg agccacttcc actacgtcca tcccaacgat gttgagatct ttcaggccgc
     3961 gtaccagttt aatggcgcga tcggaggtca ggccgccaat cactggcgta ccggttcccg
     4021 gcgcgaaagc aggatccagg cagtcgatat caaaagtcag gtaaaccggc atatcaccca
     4081 caatctgttt cacctgggcg ataacgtcat ccacgctgcg atcgttcacc tggcaggcgt
     4141 ccagcacggt aaagccgttg tctttatcaa actcggtacg aataccaatc tgcacggaat
     4201 gattcgggtc gatcagacct tctttcggcg cggtatagaa catagtgccg tggtcaaatt
     4261 cacaaccgtt cgcataggta tcggtgtggg cgtcaaagtg taccagcgcc attttgccga
     4321 aatgcttcgc atgagcacgc agcagcggca gcgtaacaaa gtggtcacca ccgaaggaga
     4381 gcatacgctt accggcagcc agcagcttct cggcgtgcgc ctgcagcttt tcgctcatct
     4441 cacgggcatc gccaaaggca tataccagat cgccgcagtc cacgacgttc agacgctcac
     4501 gcatgtcgaa attccacggg aagcggttgt gttcccaggc cagattcgtc gaaacctgac
     4561 ggatcgctgc cggaccgtgg cgaccacccg cacgaccaga agtggccata tcgaacggca
     4621 cgccagtaat cacccagtct gcatcgctgt catacggctg gaagttcatc ggcaggcgta
     4681 aaaaaccaaa ggcattggaa accagtgagt tatcgtattg atgacctaag gtgctcatgg
     4741 atggactcct attacaaagt cgatataaaa aaacccttcc gcgtcgttag gcccgacgag
     4801 gaagggttgg atttgcgaaa cacaagtttg tgctaattat cgccttaaat gggcgaggca
     4861 tcaagcgagg ttgctatttc cgacacggtg gcggcgcata accattgtta tctgccggaa
     4921 aggcttgtcc agagcacggc ggcagcgcgt tttgcgccgg gccttgtaat tcaaaggcgg
     4981 caaagccgat taccccggta ttacgcacat cgccttgcgc cgaattggct gcgtaggaat
     5041 cagcaacgtt ggcgaactga aa
//
LOCUS       DAJTNM010000202         4946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTNM010000202 DAJTNM010000000
VERSION     DAJTNM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.203"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..1655
                     /gene="mtlA"
                     /locus_tag="OO172_003992"
     CDS             <1..1655
                     /gene="mtlA"
                     /locus_tag="OO172_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5513952.1"
                     /translation="TTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDGKIKSGFE
                     MLVNNFSAGIIGMILAILAFLGIGPIVEALSKMLAAGVNFMVVHDMLPLASIFVEPAK
                     ILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAKQSA
                     GGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSIL
                     AVLAMTPKGAYFANIAGVCAAMAVSFVVSAILLKTSKVKEEDDIEAATRRMQDMKAES
                     KGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSAMGAGVLRKKIQDAGLSQISVTNSAI
                     NNLPPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERLVAAQRHTENE
                     VKVKDSFDDSSANLFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLD
                     REKLTPTYLGESIAVPHGTVEAKDRVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAA
                     RNNEHIQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK"
     gene            1750..2898
                     /gene="mtlD"
                     /locus_tag="OO172_003993"
     CDS             1750..2898
                     /gene="mtlD"
                     /locus_tag="OO172_003993"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5513953.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            2898..3485
                     /gene="mtlR"
                     /locus_tag="OO172_003994"
     CDS             2898..3485
                     /gene="mtlR"
                     /locus_tag="OO172_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5513954.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(3497..3706)
                     /locus_tag="OO172_003995"
     CDS             complement(3497..3706)
                     /locus_tag="OO172_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513955.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            3990..4352
                     /locus_tag="OO172_003996"
     CDS             3990..4352
                     /locus_tag="OO172_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5513956.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1276 a         1204 c         1246 g         1220 t
ORIGIN      
        1 ttaccaccat gggcgttatc gtcggcgcag acatgccgat gttcctcggt tctatgattg
       61 caggtccgct gggcggctgg tgcattaagc acttcgaccg ctgggtagac ggtaagatca
      121 aatccggttt tgagatgctg gtgaataact tctccgcagg catcatcggg atgatcctcg
      181 ctattctggc attcctcggc attggcccga ttgttgaagc cctgtccaaa atgctggctg
      241 cgggcgttaa cttcatggtt gtccatgaca tgctgccgct ggcgtctatc tttgttgaac
      301 cggcgaaaat cctgttcctc aacaacgcca ttaaccacgg tatcttctcg ccgctgggta
      361 ttcagcagtc ccatgaactg ggtaaatcaa tcttcttcct gattgaagct aacccaggtc
      421 caggtatggg cgtgctgctg gcgtacatgt tctttggtcg tggtagcgct aaacagtctg
      481 cgggcggtgc ggcaatcatc cacttcctgg gtggtatcca cgaaatctac ttcccgtatg
      541 tgctgatgaa tccgcgtctg atcctcgcag tcatcctcgg cggtatgact ggcgtgttca
      601 cgctgactat cctgggcggt ggtctggttt ctccggcatc tccgggttct atccttgctg
      661 tactggcgat gacaccaaaa ggtgcttatt tcgctaacat cgcgggtgtg tgtgcggcga
      721 tggctgtctc cttcgttgtc tctgctattt tgctgaaaac cagcaaagtg aaagaagaag
      781 atgatattga agcagcaact cgtcgtatgc aggacatgaa agctgagtct aaaggcacat
      841 ctccgctatc tgcgggcaat gtgactaacg acctgagcca cgtacgtaaa atcatcgttg
      901 cctgtgacgc cggtatgggt tcaagtgcga tgggcgcagg cgtgctgcgt aagaaaattc
      961 aggatgcagg tctgtcgcag atttcggtca ctaacagcgc gatcaacaac ctgccgccag
     1021 atgtggacct cgtcatcact caccgtgacc tgaccgaacg cgctatgcgc caggttccgc
     1081 aggcacagca tatttcgctg accaacttcc tcgacagcgg cctgtacacc agcctaaccg
     1141 aacgtctcgt tgctgcccag cgccatactg aaaacgaagt gaaagtgaaa gacagctttg
     1201 acgattccag tgctaacctg ttcaaactgg gcgcggagaa catcttcctc ggtcgcaaag
     1261 cagcaaccaa agaagaagcg attcgttttg ctggcgagca gctggtgaaa ggcggttacg
     1321 ttgagccgga atacgttcag gcgatgctgg atcgtgaaaa actgaccccg acttatctgg
     1381 gtgagtctat cgcggtgcca cacggtacgg ttgaagcgaa agatcgcgta ctgaaaacgg
     1441 gcgtggtgtt ctgccagtac ccggaaggcg tgcgcttcgg tgaagaagaa gatgacattg
     1501 cccgtctggt gattggtatt gcagcccgta acaacgagca cattcaggtt atcaccagcc
     1561 tgaccaatgc actggatgat gagtccgtca tcgagcgtct ggcacacacc accagcgtgg
     1621 atgaagtgct ggaactgctg gcaggtcgta agtaatccaa tcctaccctc tcctcacgga
     1681 gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac attgatgaag
     1741 gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg ctttatcggt
     1801 aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca ggtggtactt
     1861 gatgccctga atgcccgtca tagctatcag gttcatgtgg tcggtgaaac cgagcaggta
     1921 gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt tgatctgatt
     1981 gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg tattgctccg
     2041 gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc gctgaacatc
     2101 atcgcctgtg aaaacatggt acgcggcacc acgcagctga aaggtcatgt gatgaacgcc
     2161 ctgccggaag acgccaaagc gtgggtagaa gagcacgttg gctttgtcga ttccgccgtt
     2221 gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt gacggtagaa
     2281 actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc gaacatccca
     2341 ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt caccctgaac
     2401 acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac cattcgtgac
     2461 gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga aagtggtgcg
     2521 gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat ccagaaaatc
     2581 ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg ccgtcagccg
     2641 ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccac tgctcggtac gctggaatac
     2701 agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt ccgcagtgaa
     2761 gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc gcaggcggcg
     2821 ctggcacaga tttccggtct tgatgccaac agcgaggttg tatccgaggc ggtaaccgct
     2881 tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg aataacagat
     2941 tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt gtgcttgagc
     3001 gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag cttttgaccg
     3061 aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg gtgaagtatg
     3121 ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg cgtttaaaac
     3181 tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggaa ctgctgatgg
     3241 cattgcgtga agagctaaat cacgacggca acgagtacgc ctttaccgac gacgaaatcc
     3301 ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca cagtttgaac
     3361 cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct gtgcgatcaa
     3421 caatggtcct ttcactgact gagctgattt ccaaaatcag cttaaaaaaa gcctttcaaa
     3481 agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc cgacgataat
     3541 actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac ggaatcgcgt
     3601 tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc atgaaatcta
     3661 cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg atatctccgt
     3721 attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg gtaaagatga
     3781 cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt atctttttct
     3841 atagatttct taattaatca acgaattgcg tagtttttga gcatgttaat gattacgttg
     3901 cagaactatt acaatgtcat cagttgtgaa caaagcacct ggtcgcgcat actaggggct
     3961 ataaatttat ctttatcaga agccatcaca tgaaagaagt cgaaaaaaac gaaatcaaac
     4021 gtctcagcga tcgcctggac gccatccgcc accagcaggc cgatctgtcg ctggttgaag
     4081 ccgcagacaa atatgccgag ctggaaaaag agaaagccac gctggaagca gaaattgctc
     4141 gcctgcgtga ggttcatagc caaaaactga gtaaagaagc acagaagctg atgaagatgc
     4201 cgttccagcg agcaattacc aaaaaagagc aggctgatat gggcaagctg aagaaaagtg
     4261 ttcgcggact ggtggttgtg cacccaatga ccgcgctggg gcgcgaaatg ggcctggaag
     4321 agatgactgg attttctaag actacgtttt aagcccctca gacgactggt tcgatcgtat
     4381 caacccgcat ctggcttgcc ttagctggtc aatgcgggga ctctcctgtc tactcgccta
     4441 attaccaaac aattcactcc ctttacttcg atttaccgct ggttgatatt ctgaaaatgc
     4501 atttttaccg aaatagccat ctataaacaa taaaaatata tagaagatat catttgttaa
     4561 ttaacaagca tttacgtctc tttttttcgg atttaattgt gaaattttaa tcatcatatt
     4621 cacaaaatca attatctgta aaatttttcc cagacatggt ttctttcata tttgacattt
     4681 attaataatc atcattagaa tccacgaaaa ataatatatt catacaaaca cagacatctt
     4741 ttgaaacgaa tgatacattt ttatgatgaa tgcttaaaac aatatttaat cgacacacat
     4801 cactgtcatt aatgtaaagc aacatagctc accaataata caatggacaa ctctaccggc
     4861 attgatcgct ggtcggagga aataaggatt tttacatgaa cttgaagaag atttttttca
     4921 gtgctgtcac tgtttcagtt ctctgc
//
LOCUS       DAJTNM010000203         4653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTNM010000203 DAJTNM010000000
VERSION     DAJTNM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.204"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..646)
                     /locus_tag="OO172_003997"
     CDS             complement(<1..646)
                     /locus_tag="OO172_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR5513957.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPI"
     gene            complement(761..836)
                     /locus_tag="OO172_003998"
     tRNA            complement(761..836)
                     /locus_tag="OO172_003998"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Thr,seq:ggt)
     gene            complement(843..917)
                     /locus_tag="OO172_003999"
     tRNA            complement(843..917)
                     /locus_tag="OO172_003999"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(882..884),aa:Gly,seq:tcc)
     gene            complement(1034..1118)
                     /locus_tag="OO172_004000"
     tRNA            complement(1034..1118)
                     /locus_tag="OO172_004000"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1082..1084),aa:Tyr,seq:gta)
     gene            complement(1127..1202)
                     /locus_tag="OO172_004001"
     tRNA            complement(1127..1202)
                     /locus_tag="OO172_004001"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1167..1169),aa:Thr,seq:tgt)
     gene            1564..2514
                     /gene="coaA"
                     /locus_tag="OO172_004002"
                     /gene_synonym="panK"
     CDS             1564..2514
                     /gene="coaA"
                     /locus_tag="OO172_004002"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5513958.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2543..3508)
                     /gene="birA"
                     /locus_tag="OO172_004003"
     CDS             complement(2543..3508)
                     /gene="birA"
                     /locus_tag="OO172_004003"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5513959.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3505..4533)
                     /gene="murB"
                     /locus_tag="OO172_004004"
     CDS             complement(3505..4533)
                     /gene="murB"
                     /locus_tag="OO172_004004"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5513960.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1188 a         1176 c         1056 g         1233 t
ORIGIN      
        1 cgatcggcag caggaacggc ttgtcaatcg cacgctctgg ttccggaatg taagaatcca
       61 ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc agcgctttca
      121 gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga gacagaagtt
      181 cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg tcgcatttgt
      241 tcaggaacac gatgatgtac ggaacgccta cctgacgccc cagcaggatg tgctcacgag
      301 tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc atctgagcag
      361 caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg tgtgcgtagt
      421 gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca cgagcttttt
      481 cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag gttttagcca
      541 gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg ccgatagtac
      601 cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat tgtccctcta
      661 agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg acttgttgaa
      721 tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat aggcagattc
      781 gaactgccga cctcaccctt accaagggta cgctctacca actgagctat atcagcacat
      841 cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg aggtaatagc
      901 cattatacga tgcccgcatc ctagaactcg gctacctgat tttcattctg cactgaatat
      961 cgagagaagc tctctttatt cgaaccggta agtgaactta tcgtctcggg ctacgccatc
     1021 gcgtggccga aattggtggt gggggaagga ttcgaacctt cgaagtctgt gacggcagat
     1081 ttacagtctg ctccctttgg ccgctcggga accccaccgg acttgatggt gccgactacc
     1141 ggaatcgaac tggtgaccta ctgattacaa gtcagttgct ctacctactg agctaagtcg
     1201 gcatcaagta gcgcgcattc tatggagaca tgcgagttca tgcaactaaa aaattgcata
     1261 atttgtttta ttggtcacat tttatgctac acgatgaaga aacagccgaa cggagggcag
     1321 attagcacac tttttcaaca tcattgtgct caacaatgcg ctcctgctaa accataattc
     1381 tttttatcag atggaatatc tgtcacattg cttttcaacg atagcttcct ggcagagatt
     1441 ttttcttatt attcctcccc atctggtgtt accctcctgc ccattaaccc attcaacaga
     1501 actgtgacgc gccatggcaa atatcgcttt gccgatagag ctatgaccgc cagaaacatg
     1561 cttatgagta taaaagagca aacgttaatg acgccttacc tacagtttga ccgcaaccag
     1621 tgggcagctc tgcgtgattc cgtacctatg acgttatcgg aagatgagat cgcccgtctc
     1681 aaaggtatta atgaagatct ctcgttagaa gaagttgccg agatctattt acctttgtca
     1741 cgtttgctga acttctatat aagctcgaat ctgcgccgtc aggcagttct ggaacagttt
     1801 cttggtacca acgggcaacg cattccttac attatcagta ttgctggcag tgtcgcggtg
     1861 gggaaaagta caaccgcccg tgtattgcag gcgctattaa gccgttggcc ggaacatcgt
     1921 cgtgttgaac tgatcactac agatggcttc cttcacccta atcaggttct gaaagaacgt
     1981 ggtctgatga agaagaaagg cttcccggaa tcgtatgata tgcatcgcct ggtgaagttt
     2041 gtttccgatc tcaaatccgg cgtgccaaac gttacagccc ctgtttactc gcatcttatt
     2101 tatgatgtga tcccggatgg agataaaact gttgttcagc ctgatatttt aattcttgaa
     2161 gggttaaatg tcttacagag cgggatggat tatccacacg atccacatca tgtatttgtt
     2221 tccgattttg tcgatttttc gatatatgtt gatgcaccgg aagacttact tcagacgtgg
     2281 tatatcaacc gttttctgaa attccgcgaa ggggctttta cagacccgga ttcctatttt
     2341 cataactacg cgaaattaac taaagaagaa gcgattaaga ctgccatgac attgtggaaa
     2401 gagatcaact ggctgaactt aaagcaaaat attctaccta ctcgtgagcg cgccagttta
     2461 atcctgacga aaagtgctaa tcatgtggta gaagaggtca gactacgcaa ataatttgca
     2521 ggggagcgaa tactcccctt tcttattttt ctgcactacg cagggatatt tcaccgccca
     2581 tccagggttt tattattcca tcctgctcaa gtaataaagc cccctgtttg tctattccgc
     2641 gtgaaatgcc aaatatttct ttatcaccaa tgataagttt cactgggcga ttaataaaat
     2701 tatccagctt ttcccagcgc gacagataag gtgccaatcc ttcttgttcg aagagttcca
     2761 acgcagcacg taattcacgt attagcatgg ccgccaacgt attacgatca agattgatcc
     2821 ccgcttcctg cagcgtgatc cacccctgat taacgacact ctcttcaaca cggcgcattg
     2881 ccatgttgat cccggatcca atgactattt gcgccgcatc gccagttttg ccagtcagct
     2941 ccacaagaat gcctgccagc ttgcgatcct gcagatagag gtcattaggc catttgacac
     3001 gaactttatc tgctcccagc tttcgtaata cttccgccat cacgataccg ataaccagac
     3061 ttaaaccaat cgccgccgcc gggccttgtt ccagacgcca gaacatcgac aaatataagt
     3121 ttgcgccaaa aggcgaaaac catttccgcc cccggcgacc acggccagcc tgctggtatt
     3181 ctgcaacaca ggcatcgccc gatttaagct ctccgatacg atcaagaagg aactgattcg
     3241 tggagtcaat aactggcagc acggctacac taccgccatc cagctgaccc aatatctgtt
     3301 cagcattaag taactggatg ggctcaggca ggctgtatcc tttacccgga acggtaaaga
     3361 catcaacgcc ccagtcacgc agtgtctgaa tgtgtttatt aatagccgcc cggctcattc
     3421 ccagcgtttc acccaactgc tcgccagagt gaaattcacc gttcgctaac agggcaatca
     3481 atttcagtgg cacggtgtta tccttcatga aattgtctcc actgcgctca cttcacctga
     3541 tgcaccaata aagcggactt caggctcaag ccagacatta aatttttcac ccactttttg
     3601 tcttacatgg tgtgccagtt gcaccacatc ttcgcttttt gcattgtctt cattaatgag
     3661 aactaacgcc tgttgacggt gcaccgcagc cccacccatt tgcatccctt ttagctggca
     3721 ctgatcaata agccaacctg ctgccagttt tactgaacca cccgcctggg ggtaatttgg
     3781 tgctgttgga aattgcgcca gtaatgcttt agccgtttcg gcagatacaa cagggttttt
     3841 gaagaaacta ccggcattgc cattcacttt tggatcaggg agtttggtgg tgcgcatatg
     3901 acacaccgca ttaaatactt gctgtggcgt tactgttgta ggatccagac gagttaagtc
     3961 accatacgtt agtacaggtt gccactcttt tggcagacgc agacctacgg ctacaatggc
     4021 gaagcgatcc tggtattcat gtttaaaaat actgtcgcga tagccaaaac ggcactcttt
     4081 ggcagttaag cgcacttgct tgcctgtcgc cagttcaaca caatcaacat aagcgcaaac
     4141 tcgctgtaat tctacgccat aagcaccaat attctggata ggtgatgagc cgacacaacc
     4201 aggaattaat gccagatttt ccagaccagg cataccttcc tgcaaagtgt attttaccag
     4261 gcgatgccag ttttctccgg ctcctacatg taaataccac gcatcaggtt catcatgaat
     4321 ttcgatacct ttgatccggt tgatgatcac cgtgccgcga tagtcttcca gaaaaagtac
     4381 attacttcct tcacccagaa taagaacggg ttgtccttct gcggttgcat gctgccaggc
     4441 attgagtaat tgttgttcgt cttcggcaca tacaatgtgc tgagcattat gatcaatgcc
     4501 aaatgtgttc cagggtttta aggagtggtt catagctgct ttcctgatgc aaaaacgagg
     4561 ctagtttacc gtatctgtgg ggggatggct tgtagatatg acgacaggaa gagtttgtag
     4621 aaacgcaaaa aggccatcct gacggatagc ctt
//
LOCUS       DAJTNM010000204         4508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTNM010000204 DAJTNM010000000
VERSION     DAJTNM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.205"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            368..2020
                     /locus_tag="OO172_004005"
     CDS             368..2020
                     /locus_tag="OO172_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5513961.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="OO172_004006"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="OO172_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2334..2453)
                     /locus_tag="OO172_004007"
                     /pseudo
     CDS             complement(2334..2453)
                     /locus_tag="OO172_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..2961)
                     /locus_tag="OO172_004008"
     CDS             complement(2527..2961)
                     /locus_tag="OO172_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HCR5513962.1"
                     /translation="MDTFKRRNVATSFSFLGYDFKVRTLKNFKGELYRKCMPGASNAA
                     MCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSR
                     LLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            complement(3138..4447)
                     /gene="ltrA"
                     /locus_tag="OO172_004009"
                     /pseudo
     CDS             complement(3138..4447)
                     /gene="ltrA"
                     /locus_tag="OO172_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1197 a         1092 c         1038 g         1181 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagaaag gagaaactcg tagcgacgtt
     2941 tctcctttta aacgtgtcaa tgtaggcgat attcgtcttc cctgcgttca ggaccaaccc
     3001 gacttccgag aagcgcaccg ataacctgtt cttcagcctt gttgcatcac tcattcgtga
     3061 acaatgtact acaagtcgag tcacccgagg gaacctcccc ctctggtgct cacggaaccg
     3121 gacgtgaacc tctcagctca tccggctctt ctcattgagg cctgtcccca tagtaacggc
     3181 cagtgtgcaa ataacctcgt ttcactccgc gcaacacggg ccagccagtg gctggcccgt
     3241 cgtcggtggc ccctaagtcg cttgtgttta cggggcagcc ataacacgag tttgcgatcg
     3301 atttgcctca gcgtcagata cagtgctgat ttataaaaag caccgtagta ctttatccag
     3361 cctttgattt tcgcattgaa catatgcgcc agctcgtaca aggacttgtc actgcgattc
     3421 tcctcacctc ctgacggatt gcttttcctg ctctggcact catcgccgga ctgaagttaa
     3481 cgaaggtctt accccagcga ttcatcgaca gacgcggccg gaatgtatag cccagaaagt
     3541 tgaagctcgt ttccgggtag tccccccgtc ggtcagcatc cttacagtaa actattcgtg
     3601 ttttttgcgg atgcagctgc agcccgcatt gcgcaagccg ctgcctcagt ccacttatca
     3661 gagcgtcagc ctgccactgg ctgtgacagt ggcatacaac atcatcggcg tacctttcga
     3721 agggaacgcc gggaaactga cgttgcatcc acatatcaaa ggcatagtgc aggaacagat
     3781 ttgagagcag gggggagatt acgccgccct gcggcgttcc tcgaccccgt gccagcattg
     3841 ctccatccgg catttgcacc ggtgccttca gcctgcgctc aatgtacatc accacccagc
     3901 gtgcctgagt atgatgctgc actgtcttca gtagcaggtc atgatcgata tcatcaaaga
     3961 agcctttaat atcgacttcc accacccagt caaacttcca gcagcgtttc ctcgcctgcg
     4021 caatcgcctg gtgtgcggat ttacccggtc gataaccata cgagtctgca tggaacaggg
     4081 gttccagtac cggctcgagt atctgcttaa cgaccatctg ggcgatccgg tccgacacgg
     4141 ccggaatacc gagcggacgt acaccaccgt cagctttggg aatgtccaca cgccttaccg
     4201 cctgcggcat ataactgccg gaagccattc tgttccacag tttgtagaga ttgtctgtaa
     4261 cattctcgtc aaacccggcc agtgtctgcc cgtcaatgcc tgccgcccca cggttggctt
     4321 ttactcgttt ataagcttcc catacctgtc gtttacttac aggaaatggc tttgccgatt
     4381 ttacagcagt cctcccatcg gcattgtcgc ccgtggttgc cgccgcacca tcagcacaat
     4441 ggggcaagcc ctttgctccg gacccattac ggtccttcac cgctactacg gtttactccg
     4501 tccctgta
//
LOCUS       DAJTNM010000205         4459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTNM010000205 DAJTNM010000000
VERSION     DAJTNM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.206"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(1..394)
                     /locus_tag="OO172_004010"
                     /pseudo
     CDS             complement(1..394)
                     /locus_tag="OO172_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1084..2793)
                     /locus_tag="OO172_004011"
     CDS             complement(1084..2793)
                     /locus_tag="OO172_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5513963.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(3225..3944)
                     /locus_tag="OO172_004012"
     CDS             complement(3225..3944)
                     /locus_tag="OO172_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5513964.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(4069..4459)
                     /locus_tag="OO172_004013"
                     /pseudo
     CDS             complement(4069..4459)
                     /locus_tag="OO172_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1238 a          962 c          832 g         1427 t
ORIGIN      
        1 gcatgctgta cccggtcgta ataaccgctc ctggatacac caggtacccg acacatcgct
       61 tcaacaggga attgttgtcg ccattgttcg actaacgcgt atttttcagc gactcctggg
      121 caaaatacgc tgttgctttt tttaatatat ctcgctcaag tcgtgcttca tttaaagcct
      181 tacgcagttg cataacttca gattccagct cagccactgt gcgggagcct ggtgtattga
      241 gcccttttct tgcggcggta acccattgtc ctaaagtgcc ttcaggaaga gataatcggg
      301 aagcgccttc actgatcgaa agctgatttt caggaaccgt tttgacggct tcggctttaa
      361 actccgtaga gtaacgttgg ttttttctgc tcattattgg ctccttctga tgccattcta
      421 tttcaggaag gagtgtccgt taaactcagg ctacctcaga taagcttgat acaactggtc
      481 tggtatatac agtttctttg agtgcggtta cgtccacaga ggtaacggta aaagtaaccc
      541 cttgtcggga ccactccgac cgcgcacaga ggcattttca tcgctgacag gccgcattgc
      601 ctcgcagggc tggaggagga tcggtgatat ccggacgacc gtaccggctt acgccgggct
      661 tcggcggttt tactcctctg ccatgtgcaa caacggagtg ccgccttaca tgccgctggc
      721 gcggcatatc ctggtaacat ttttgttgat accaccttcc ggccttatcc tgaccagtgg
      781 gctttcctgg catcgatgat accgatgaac gcgcatgata ttgagccgac gatattacgg
      841 gcaactggaa atactcactc tctggatgtg acctttatcc atgaagaaga tttggccaca
      901 ccatggaaac ctgaacaatc atcagtttac gcccatgtaa acccctacgg gatatttgaa
      961 ctcgatatgg aaacaaggtt gccaatcgag gtggttgcct gaaagttggc agttacccgg
     1021 tcactacacc ttgttggagt gaacatatca aaaacaggta attacacaat ttaaattaca
     1081 gtctcataaa aaatgattaa atctatttat tatttcaata aacgtttttt tcatcttctt
     1141 tgaaaattgt tctgctatag tatccggttc cagattgttc tcttttatta ttctacaata
     1201 ctctaatatt gtttctttgt ctctatttac tacagataca aatacaccag gaataccatc
     1261 tttattagtt gcggacagca tttcgaccaa tcgtcttgtg gtaaaactat agttttttag
     1321 aatattacaa aaggcacgta ttgtttcggt atggccatta taaaatgcca taaaaagtcc
     1381 tgatgtaccg ttgggatttt ttatccataa taattcatct atatattcct ggtgaggggg
     1441 gagtattttt agtatatctc catacgccat aatggccttt gtgtgcccat tttgaagtgc
     1501 cataaacaac ccatgcttta ctttcaataa ttctttaatt tgttcactac taagcatttt
     1561 cttattaagc agacttccga acatacgaat actatctgca tgtccattct gcaatgcaaa
     1621 aaataaccca ggtaaatcat ggctgtcttt cgcagctaat agatataatc tcttatcgga
     1681 agttaacggg gttttaaaga ctatttcccc ataggctttt attgtgtcta catgtccgtt
     1741 cataaaagac atgcataaac ccggcacatt gtttgcccct accgcagcaa gcaatgtaag
     1801 tattgtttct tgactaagat tgcacatatt taatatctta ccatatactt gaataatgtc
     1861 tgaatatccg ttttgaagtg ctatataaaa tgcaggagta ccatcagagc ttctcgtggc
     1921 cagaaggttc accagtttag attgagaaat ttcaccattt ttgtattttt ccatcagtat
     1981 aaataatact tctgtcagat tctttgttat cgcatgatat ataactctgg aattgatcaa
     2041 ttcatctggt aaaagtttca ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat
     2101 tacctgcccg ggttgctcca gaccataaat tttccaataa aaatcatcaa aaaaatcctt
     2161 agcggataat tgactcagtt cgaaatggct atcacaagta aatacagtac gtttatgagt
     2221 aacagttcga ttaggatcga aaaactgaac aacccatttt tcactttctt gtacgcgctt
     2281 tattttcaac cctaacgcca tttggtggtt taatgtgcat gctgataatg ttctgacaaa
     2341 gtctccattt tttttcatgt tataaaaaaa attagtaata actgttcccc actttttatt
     2401 ttccacaaaa taaatataag ctggtgattt tgaaaaatct tccgtttttt ccaaacatac
     2461 attttttgca atagcatcag gggtgctaag aagctgatag tttaccttcc catttgtttc
     2521 cataaaattc atttcccaat aggaagctag gtgacggcaa actatttgtc ttccatatat
     2581 atcttttgcc actccattta tatttacctt cacatttctc ttttctgaaa aataaggtgc
     2641 tgttttattc ttaaactgaa ttagttcact ggagtttgcg accctcataa tagaacttgt
     2701 tattgttcta ttatctctgt taacagacat atctgttgat aacgaatttt ttgttgaaaa
     2761 tatactagat gaaaatgttt tatttaacgg catagaagac aaccatagag atgttataat
     2821 actattagat tgcagttact aatagtaaaa gcctccttac aaggaggtag cttattcaga
     2881 atgctcctgt aagatagcga gctcgtcaac gatacagatg atctgattat attgtgcaaa
     2941 aggagaacag gaaaataggc actggaaaag atgaaggcct gatgggagca ttaactaatg
     3001 caaaatccga caggttaata tcccgaaaag caatcaaacg actcaaacag gagatgcacg
     3061 ctgaagtggt ggacaactgt tcggatttca gatgagtagc tcagcagttt atgagccgaa
     3121 tgctctgtca cgggcaggaa tcaggctgca tacgcagtgc caaatctaat cgtagttatt
     3181 ctaaccagtt ttcaagctac aaggtggtgt agctggcatc ttctctactc tatcatcaaa
     3241 cgataaaatg gttcctctcg taacatttgc tcttgccttt cacttccatc tcgatcgctt
     3301 cgtagttcat tacgatagct gacatatttc cagtcttcag gtctaacatc tgacgccgga
     3361 tactccccgg gagcgatttt gcttcttaat agattagact cgagacacat tataaattgt
     3421 cgtattttat gaagtaataa agcactatat tgcgagcatt cctgatctga ttttacatat
     3481 agcgtaaact gagctcctat ccccacacga gattgctgag aaactcgatt catatcagtt
     3541 atcttccatt tatctatagg actgtcctct gaaaataata aaccagacaa aatttggaat
     3601 gccagaggaa cttgctccct agctatacta atatgaaact tgtccccaac aaaatccccc
     3661 ttactctgag gattttcacg acatgcatga ataaacacat ctccactatg gttcacaatc
     3721 tggaatcctt ggtacatgac agaaaaaaat tgacatatat cagagtaagt tggcacgttc
     3781 gatgctactt tgaaatagta cgctgggtat tgccgtattt ggttgtacaa tatattgaag
     3841 ttatttttca atctttcatt cgcagaaagc atctgcggta tttcagacct cacaacattc
     3901 aaagaatcta aatttagttt tagacagggc ttttttatgg gcatagaaaa cgtcctctat
     3961 aaaatagata tattatctta tattgattgt ttatcatctt ttataaaacg atacattata
     4021 tgtaatgtat gtaagcgtaa gggtcttctc cgatcatgat gggaaggctt agaacgccat
     4081 attcagtttc ccatagtgga acatcactcc cagcttaaag cgctcccggt tgcggtagcc
     4141 cttggctttt attctcagca gcctgatctt gctgttcagt gcttccgcat ttccgtttga
     4201 tacgccatgg cgcatggcgt tcaggatacc gtacaacctt ttccctatcg ttttagccac
     4261 attcttcatc atggggacgt cactgttggc cgcagccatc tctgccagtc gttgcgtctt
     4321 tcctcgctcc atggtctgct ccagatatcc tttgccagtt ctttcagcgc ccagcacagg
     4381 ctcgtcagct tcatctgcgc acgcagccat atcagctttt cctgcctgga ttcggtcatc
     4441 catttatcgc tgtgctgcc
//
LOCUS       DAJTNM010000206         4453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTNM010000206 DAJTNM010000000
VERSION     DAJTNM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.207"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            261..854
                     /gene="tetC"
                     /locus_tag="OO172_004014"
     CDS             261..854
                     /gene="tetC"
                     /locus_tag="OO172_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5513965.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(967..2172)
                     /gene="tet(B)"
                     /locus_tag="OO172_004015"
     CDS             complement(967..2172)
                     /gene="tet(B)"
                     /locus_tag="OO172_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5513966.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            2254..2877
                     /gene="tetR(B)"
                     /locus_tag="OO172_004016"
     CDS             2254..2877
                     /gene="tetR(B)"
                     /locus_tag="OO172_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5513967.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2855..3541)
                     /locus_tag="OO172_004017"
     CDS             complement(2855..3541)
                     /locus_tag="OO172_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5513968.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(3549..3935)
                     /locus_tag="OO172_004018"
     CDS             complement(3549..3935)
                     /locus_tag="OO172_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5513969.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            complement(3928..4191)
                     /locus_tag="OO172_004019"
     CDS             complement(3928..4191)
                     /locus_tag="OO172_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5513970.1"
                     /translation="MSWSHYRPLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVL
                     QWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            complement(4188..>4453)
                     /locus_tag="OO172_004020"
     CDS             complement(4188..>4453)
                     /locus_tag="OO172_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5513971.1"
                     /translation="ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQR
                     IERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1433 a          961 c          811 g         1248 t
ORIGIN      
        1 ttggtataac ccgctttatt cgccacatca tcaagcagta aagactgatc taaattatgt
       61 tcaatccata atagaacatc tttgataacc gttgtatgaa actgcttatc atcatatctt
      121 aattggatgt tattagcttt attttgatag cgagaatgct gttcaatata cataaaataa
      181 cctaaatgtt cttaagattg tcacgaccac atcatcatga taccataaac atactgacgg
      241 tatgttattt taaatctatc atggaaaata aaaatcatca acaagaaaat tttaagagta
      301 cctatcaatc actggttaac tcagcacgaa tattgtttgt tgaaaaaggc tatcaagctg
      361 tttcaataga tgagatctcg ggaaaagcgt tggtgaccaa aggtgccttt tatcatcact
      421 ttaaaaataa aaaacaatta ctcagtgcct gttataagca gcaattaatt atgattgatg
      481 cctacatcac aacaaaaact gatttaacaa atggttggtc tgccttagaa agtatatttg
      541 aacattatct tgattatatt attgataata ataaaaacct tatccctatc caagaagtga
      601 tgcctatcat tggttggaat gaacttgaaa aaattagcct tgaatacatt actggtaagg
      661 taaacgccat tgtcagcaaa ttgatccaag agaaccaact taaagcttat gatgatgatg
      721 tgcttaaaaa cttactcaat ggctggttta tgcatatcgc aatacatgcg aaaaacctaa
      781 aagagcttgc cgataaaaaa ggccaattta ttgctattta ccgcggcttt ttattgagct
      841 tgaaagataa ataaaataga taggttttat ttgaagctaa atcttcttta tcgtaaaaaa
      901 tgccctcttg ggttatcaag agggtcatta tatttcgcgg aataacatca tttggtgacg
      961 aaataactaa gcacttgtct cctgtttact cccctgagct tgaggggtta acatgaaggt
     1021 catcgatagc aggataataa tacagtaaaa cgctaaacca ataatccaaa tccagccatc
     1081 ccaaattggt agtgaatgat tataaataac agcaaacagt aatgggccaa taacaccggt
     1141 tgcattggta aggctcacca ataatccctg taaagcacct tgctgatgac tctttgtttg
     1201 gatagacatc actccctgta atgcaggtaa agcgatccca ccaccagcca ataaaattaa
     1261 aacagggaaa actaaccaac cttcagatat aaacgctaaa aaggcaaatg cactactatc
     1321 tgcaataaat ccgagcagta ctgccgtttt ttcgccccat ttagtggcta ttcttcctgc
     1381 cacaaaggct tggaatactg agtgtaaaag accaagaccc gctaatgaaa agccaaccat
     1441 catgctattc catccaaaac gattttcggt aaatagcacc cacaccgttg cgggaatttg
     1501 gcctatcaat tgcgctgaaa aataaataat caacaaaatg ggcatcgttt taaataaagt
     1561 gatgtatacc gaattcgatt gcgtctcaac ccctacttcg gtatctgtat tatcacgtgt
     1621 atttttggtt tcacggaacc aaaacataac cacaaggaaa gcgacaatat ttagcaacgc
     1681 agcgataaaa aagggactat gcggtgaaat ctctcctgca aaaccaccaa taataggccc
     1741 cgctattaaa ccaagcccaa aacttgcccc taaccaaccg aaccacttca cgcgttgaga
     1801 agctgaggtg gtatcggcaa tgaccgatgc cgcgacagcc ccagtagctc ctgtgatccc
     1861 tgaaagcaaa cggcctaaat acagcatcca aagcgcactt gaaaaagcca gcaataagta
     1921 atccagcgat gcgcctatta atgacaacaa cagcactggg cgccgaccaa atcggtcaga
     1981 catttttcca agccaaggag caaagataac ctgcattaac gcataaagtg caagcaatac
     2041 gccaaagtgg ttagcgatat cttccgaagc aataaattca cgtaataacg ttggcaagac
     2101 tggcatgata aggccaatcc ccatggcatc gagtaacgta attaccaatg cgatctttgt
     2161 cgaactattc atttcacttt tctctatcac tgatagggag tggtaaaata actctatcaa
     2221 tgatagagtg tcaacaaaaa ttaggaatta atgatgtcta gattagataa aagtaaagtg
     2281 attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa
     2341 ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg
     2401 gctttgctcg acgccttagc cattgagatg ttagataggc accatactca cttttgccct
     2461 ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct
     2521 ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag
     2581 tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat
     2641 gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa
     2701 gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta
     2761 ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc
     2821 cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtcttaaaag
     2881 cagcataacc tttttccgtg atggtaactt cacggtaacc aagatgtcga gttaaccacc
     2941 ctttagattc ataaagcgaa aataatgcgg ctccaacgta cccacctaaa tggaaacggc
     3001 gttcactcca atctaaacac gcacaacaga ttttacgtga atgtttggaa ggaacgtcaa
     3061 ttcccatttc atgaaaatat tgaataccac ttaatgtgat cattgaacca ttttcagtga
     3121 tccattgctg ttgacaaagg gaatcataga tcttaacggc aacttcgcca gctaaatgat
     3181 catagcaagt acgtgctttt cgtaaatgca ctggcgtgga aactttggca tgtacgccat
     3241 ggtttaagga gatccccatc atactttcca tcaattcagc aatatctttt cctgctagcc
     3301 gaaaataacg atgcttgcct tgagctacta ctgtgattag ctggcaatct aataatttag
     3361 ataaatgact gctcgccgtt gaagctgata tattcgccac agaacttagc tcagtggccg
     3421 tccaagctcg cccatccatc aaagcactga gtattttaac tcgtgaaatg tcagacatag
     3481 ccgcccctat cgcggctatt gaggactcaa aggtaacctc ttttcgtatt aaattagcca
     3541 tcgcaagttc actttattgc ccaagggagc gtaacagatg cagccatact atcattgtcc
     3601 gttattaata tcagttggtt agcatggtca ctgtattgca ctaaaatatt aatgttattc
     3661 tccgccaata ctcgtgctat ttcgccaagt tcccccggtt tttcctgttt taacttacga
     3721 attaatggtg tccggatcgc aagtactaac agtccagctt gctctagcgc tattttagct
     3781 tcctttcctt gttcaacaag aaaatgagca tggcattcat caccaaccgt aaatatccct
     3841 cccccttcca atccaatacc tttattacct aatgtttttc ctagtaatgc taattgtcct
     3901 atttgattat ctaaaacaac gtgaacatca aacattatcc ttgtcctttt taaatgaata
     3961 ttcgcgagta atatgagcaa tactaatttt gtaaaaatca aatattgact ctcgcccttc
     4021 ttgttgagct ttcgcatgta aaacatgatt tttccattgc agaactgcgt attcgttttc
     4081 ccaccaagat agcgataaca tttttccttc tgtagctaga ctttgaaaac gttcaattga
     4141 aataaaacca gctacatgac ttaatagtgg tcggtaatga ctccaactta ttgatagtgt
     4201 tttatgttca gataatgccc gatgactttg tcatgcagct ccaccgattt tgagaacgac
     4261 agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt
     4321 cgctgcgtat atcgcttgct gattacgtgc agctttccct tcaggcggga ttcatacagc
     4381 ggccagccat ccgtcatcca tatcaccacg tcaaagggtg acagcaggct cataagacgc
     4441 cccagcgtcg cca
//
LOCUS       DAJTNM010000207         4445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTNM010000207 DAJTNM010000000
VERSION     DAJTNM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.208"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..252)
                     /locus_tag="OO172_004021"
     CDS             complement(<1..252)
                     /locus_tag="OO172_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5513972.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPVTLFVPLIMGC"
     gene            562..2334
                     /gene="aspS"
                     /locus_tag="OO172_004022"
     CDS             562..2334
                     /gene="aspS"
                     /locus_tag="OO172_004022"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5513973.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGVSLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2452..2904
                     /gene="nudB"
                     /locus_tag="OO172_004023"
     CDS             2452..2904
                     /gene="nudB"
                     /locus_tag="OO172_004023"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5513974.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2933..3673
                     /locus_tag="OO172_004024"
     CDS             2933..3673
                     /locus_tag="OO172_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5513975.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3708..4229
                     /gene="ruvC"
                     /locus_tag="OO172_004025"
     CDS             3708..4229
                     /gene="ruvC"
                     /locus_tag="OO172_004025"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5513976.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4231..>4445)
                     /locus_tag="OO172_004026"
     CDS             complement(4231..>4445)
                     /locus_tag="OO172_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5513977.1"
                     /translation="CIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSIPWERKTVTPAS
                     LWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1102 a         1107 c         1239 g          997 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacgggggca tcaaccggct gttttaatgc
       61 ttcggcgtaa tcggcagacc agccaactcg caccagaaac acgggctgac cgctggcgcg
      121 aaatttcgcc gccagcttcc cggcgcgatt aactacctca tcggcagtat gtggaccacc
      181 ggcaaaaggt aagatgcctt cttgtaaatc gatcaccacc aacgcagtgg ttttagcatt
      241 aagttcaagc atgctgactc ctgaaaaaac aaaatcgtgc ctcacacctt aatgcgatta
      301 gccgatggcg gaacgacaaa ttttgttaat ttttatgagt tcgggcaaca cctttatagc
      361 aaactggcgt agagtctgat ttcaggcccg atataaagtg gtaacgaggc gttccagcgc
      421 gggccaggct cttacggtgc ggcagaattt ccagtataat agccgccttt tttcattcag
      481 ttgtgacata cagctaacgc tgcgacttgg tcacctgcgc agcagggggc atccgccggc
      541 agctaagtta agggatatct catgcgtaca gaatattgtg gacagctccg tttgtcccac
      601 gtggggcagc aggtgactct gtgtggttgg gtcaaccgtc gtcgtgatct tggtagcctg
      661 atcttcatcg atatgcgcga ccgcgaaggt atcgtgcagg tatttttcga tccggatcgt
      721 gcggacgcgt taaagctggc ctctgaactg cgtaatgagt tctgcattca ggttacgggc
      781 accgtacgtg cgcgtgacga aaaaaatatt aaccgcgata tggcgaccgg cgaaattgaa
      841 gtgctggcgt cctcgctgac tatcatcaac cgcgcagatg ttctgccgct tgactctaac
      901 cacgtcaaca ccgaagaagc gcgtctgaaa taccgctacc tcgacctgcg tcgcccggaa
      961 atggcgcagc gcctgaaaac ccgcgctaaa atcaccagcc tggtgcgccg ttttatggat
     1021 gaccacggct tcctcgacat cgaaactccg atgctgacca aagccacgcc ggaaggcgcg
     1081 cgtgactacc tggtgccttc tcgtgtgcac aaaggtaaat tctacgcact gccgcaatcc
     1141 ccacagttgt tcaaacagct gctgatgatg tccggttttg accgttacta tcagatcgtt
     1201 aaatgcttcc gtgacgaaga cctgcgcgct gatcgtcagc ctgaatttac tcagatcgat
     1261 gtggaaactt ctttcatgac cgcgccgcaa gtgcgtgaag tgatggaagc gctggtgcgt
     1321 catttgtggc tggaagtgaa gggcgtggat ctgggcgatt tcccggtaat gacctttgcg
     1381 gaagcagaac gccgttatgg ttctgataaa ccggatctgc gtaacccgat ggaactgacc
     1441 gacgttgccg atctgctgaa atctgttgag tttgcagtat ttgcggggcc ggcgaacgat
     1501 ccgaaaggcc gtgttgccgc tctacgtgtt ccgggtggcg tatcgctgac ccgtaagcag
     1561 atcgacgaat acggtaactt cgttaaaatc tacggcgcga aaggtctggc ttacatcaaa
     1621 gttaacgaac gcgcgaaagg tctggaaggt atcaacagcc cggtagcgaa gttccttaat
     1681 gcagaaatca tcgaagccat cctggatcgt actgccgcgc aagatggcga tatgattttc
     1741 ttcggtgccg acaacaagaa aattgttgcc gacgggatgg gcgcactgct cctgaaagtg
     1801 ggtaaagacc ttggtctgac cgacgaaagc aaatgggcac cgctgtgggt tatcgacttc
     1861 ccgatgtttg aagacgacgg tgaaggcggc ctgacggcaa tgcaccatcc gttcacctca
     1921 ccgaaagaca tgactgctgc agaactgaaa gctgcaccgg aaaatgcggt ggcgaacgct
     1981 tacgatatgg tcatcaatgg ttacgaagtg ggcggtggtt cagtacgtat ccataatggt
     2041 gatatgcagc agacggtgtt tggtattctg ggtatcaacg aagaggaaca gcgcgagaaa
     2101 ttcggcttcc tgctcgacgc cctgaaatac ggtactccgc cgcacgcagg tctggcattc
     2161 ggtcttgacc gtctgaccat gctgctgact ggcaccgaca atatccgtga cgttatcgcc
     2221 ttcccgaaaa ccacggcggc agcgtgtctg atgactgaag caccgagctt tgctaacccg
     2281 actgcactgg ctgagctgag cattcaggtt gtgaagaagg ctgagaataa ctgatatgac
     2341 tcaaatacac gaaatcattc gcgttgcatc aaggcggcaa ctgagtgaac tcccatgagc
     2401 atagataact atgtgaatgg gatgagcgaa ggcagtcaac gaagaggcag cgtgaaggat
     2461 aaagtgtata agcgtcccgt ttcgatctta gtggtcatct atgcacaaga tacgaaacgg
     2521 gtgctgatgt tgcagcggcg tgacgatccc gatttctggc agtcggtaac cggcagcgtg
     2581 gaagagggtg aaaccgcgcc gcaagctgcc atgcgcgaag taaaggaaga ggtcaccatt
     2641 gatgttgtcg ctgaacaact gaccttaatt gactgtcagc gcacggtaga gtttgaaatt
     2701 ttttcacatt tacgtcatcg ctatgcgccg ggcgtgacgc gtaatacgga atcatggttc
     2761 tgtcttgcgc ttccgcacga gcggcagatc gttttcactg aacatctggc ttacaagtgg
     2821 cttgatgcgc ctgctgcggc ggcgctcact aagtcctgga gcaaccggca ggcgattgaa
     2881 cagtttgtaa ttaacgctgc ctgaacaggc agggctatct ggagatattt ttatggcagg
     2941 tcatagtaaa tgggccaaca ccagacatcg taaagctgcg caggatgcta agcgcggtaa
     3001 aatcttcact aaaatcattc gtgagctggt aaccgcggct aagctgggcg gtggcgatcc
     3061 ggacgctaac ccgcgtctgc gtgcggcggt ggataaagca ctgtctaaca acatgacccg
     3121 tgacacactg aaccgcgcaa ttgcacgtgg tgtgggcggt gatgatgatg caaacatgga
     3181 aaccatcatc tacgaaggtt acggtcctgg cggcacggca atcatgattg aatgtctgtc
     3241 tgacaaccgc aaccgtaccg ttgctgaagt gcgtcacgca ttcagcaaat gtggcggtaa
     3301 cctcggcact gacggttccg tagcttatct gttcagcaaa aaaggcgtga tctccttcga
     3361 gaaaggcgac gaagacacca tcatggaagc agctctggaa gcaggtgctg aagacgttgt
     3421 gacatatgat gacggcgcga ttgatgtcta cactgcatgg gaagaaatgg gtaaagtgcg
     3481 cgacgctctg gaagcggcag gtctgaaagc agacagcgcg gaagtttcca tgatcccgtc
     3541 caccaaagct gatatggatg cagaaaccgc gccgaaactg atgcgtctga tcgatatgct
     3601 ggaagattgc gacgacgtgc aggaagttta ccataacggt gaaatctctg atgaggttgc
     3661 agcgactctc tgatgatgcc tgctaaacag caaaacggag acgcgtgatg gctattattc
     3721 tcggcattga cccgggttcg cgcgtgaccg gctacggcgt catccgccag gtaggtaggc
     3781 aactgtctta cctgggtagc ggatgcatac gcaccaaagt ggatgattta ccgtctcgtc
     3841 tgaagctcat ctatgcgggc gtgacggaaa tcatcaccca gttccagcct gattatttcg
     3901 ccattgaaca agtctttatg gcgaagaacg ctgactcagc cctgaaactg ggccaggcgc
     3961 gcggcgtggc gattgttgcg gcggtgaatc aggaattgcc ggtatttgaa tacgcggcac
     4021 gtcaggtaaa gcaaacggtg gtaggtatgg gtagtgccga aaaaagccag gtgcagcata
     4081 tggtccgcac cttgctgaaa ctgcccgcta atccacaggc ggatgccgcc gatgcgctgg
     4141 cgattgccat cacccactgc cacgttagtc agaatgcgat gcagatgagc gaatcgcggc
     4201 tgaacctggc gagagggcga ctgcgttaag ttataccgcc tcggtcagtt ctggctgagg
     4261 cgtttcaact ccctccgcgt gaatcaacac caaacccgga tgatgccaca aactggctgg
     4321 cgtgacggtt ttacgctccc acggaataga acctaagaac cagaatttcc agaaagtgag
     4381 ttttgcattc ggattgctat ttaacagttg tccaaacgtc tctatttcac ccaccggaat
     4441 acata
//
LOCUS       DAJTNM010000208         4406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTNM010000208 DAJTNM010000000
VERSION     DAJTNM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.209"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..193)
                     /locus_tag="OO172_004027"
     CDS             complement(<1..193)
                     /locus_tag="OO172_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HCR5513978.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLA"
     gene            complement(204..2501)
                     /gene="xdhA"
                     /locus_tag="OO172_004028"
     CDS             complement(204..2501)
                     /gene="xdhA"
                     /locus_tag="OO172_004028"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5513979.1"
                     /translation="MEAREATATGESCMRVDSIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATHTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASTGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAFESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            2916..3671
                     /locus_tag="OO172_004029"
     CDS             2916..3671
                     /locus_tag="OO172_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5513980.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3750..3823
                     /locus_tag="OO172_004030"
     tRNA            3750..3823
                     /locus_tag="OO172_004030"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3782..3784,aa:Gly,seq:ccc)
     gene            3958..>4406
                     /locus_tag="OO172_004031"
     CDS             3958..>4406
                     /locus_tag="OO172_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5513981.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKG"
BASE COUNT         1113 a         1098 c         1128 g         1067 t
ORIGIN      
        1 ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata tggcgataac
       61 ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc agtttggcct
      121 gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga tggtaagaag
      181 caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc ttcatataac
      241 cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt caccgcgtta
      301 cgaatagcag cggcaacagg aattatgggt ggctcaccca gtgacttatg tccgtatgcg
      361 gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc cggcatggtc
      421 ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttagc atcgatgatc
      481 atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac ctgaccttct
      541 gccagcagtg gattaagaat atgccctgaa tcatgaacgt tgaggatgcg gttgatggtg
      601 actttgcaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa cgccggtggg
      661 ttagtggtgg ttttgatgga gctttcagca gagagctgcc cgccgcgttc agggtggtag
      721 aaagcgtcca tcgccaaatc ttttaacgac attaacggct cttccggtcg ttcaaccagc
      781 acgatatggc cttttatcag ggtcaaattc atcgctgact gatgtagcat gactgcggcg
      841 tgagcgatga ttttctcttt taataatagt gccgcactgc gcagcgcagg cgcggcaaca
      901 tagctctggc gtgaggcaaa tgcgccggga tcgaacggcg taacgtcggt atcttgtgtt
      961 gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgccaccat ttgcgagaag
     1021 acggtgtcgg caccctgacc gatttccgtc gcgccgcttt gcacgttgat ggttccatcc
     1081 tgattcatca gaaggcgcgc gcctgctatt tctacgccga caggccaggt gttagaggtg
     1141 tagctaaaac aggcgacgcc aacgccgcgg cgcaaattgc cttgctggtt ctggcattct
     1201 gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc cggcaaccct
     1261 gcgctgcaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc ggcgttgcgt
     1321 aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag catagactca
     1381 aaggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga ggggaggttg
     1441 gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta agcgacttta
     1501 ttcccccccg tagaagcgat ggagtgcccg tgagatgcat aagcgccggt gttagacaga
     1561 acatccagac tataaccttt caaggttccg tcgcggttca cgcccatttg cccgtcaata
     1621 gtaaaagcgt ggcgggtatg ggttgcgagg aaacactctt cacggctaag ggaaactttc
     1681 accggaatgc cgccaagctt gctggtcagg aatgccgcca ttggctcttc cagtacatcc
     1741 tgtttattac caaaaccgcc accgacaaac ggtttgatga ctcgtacgca tgaccaggga
     1801 atatccagcg cctgaccaac cacgcggcga acaatgtgcg ggatctgggt gctggaaacg
     1861 atggtaattc gcgagtcatc ctccatccac gccagcgatg ttacgctttc catgtgacaa
     1921 tgttgaataa cgggagtctg atagtgcccc tgtacctggt agtcggcggc atcgattgtt
     1981 tgttggacat tgcccgtcga catcgtgctt tgtttcagta aattgccgcc gttatggatt
     2041 ggtgcagcgt cttctgtcag cgccgcttct ggcgtggtga taacgggtaa ttcttgccac
     2101 tcaatgctga ccaattgcgc cgctttttct gccgtgagtt catcgcgggc cacgacgatg
     2161 gcaacggcgt caccatgatg acgaacatgg cgagttagca gtgcgcgatc ggcggtatcg
     2221 cgcttgtttt cgtcaagtgt ccaggcatgc ccagctgtag cgaatggaat atcaggcaca
     2281 tcttcccaag taaaaatcgc cagtacgccc ggtaaacttc tggcttgttc atcattaata
     2341 cttacggcat aaccatgtgc gatagggcta cgtacatatt tcgcgtaaca catgcccgcc
     2401 ataacataat cgtcagtata tcgtgcccgc ccggtgacct tagcaatgga atcgacgcgc
     2461 atgcatgatt cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc ctcgatttga
     2521 acatttggtt cttatggcaa agatttagtc taagcaggca agatttacgc cagaaacgac
     2581 gataagcgtt aggggaggaa ggaagtgtga tgacgttaaa aaaacacaga tatgatacga
     2641 aacaggactc tgaataggga cagtgatacg tatcaatatg agtggcgata aaatcaatgt
     2701 gataaatcag gattattacg catcaggatg ggaaataggg taaaaagtca ggaacttcga
     2761 tacctctaca gcgaatattt tgctgatgat acttcccttt gccaaatgaa attaacctct
     2821 ttctctatta ccacgttttt ccagaagcaa gagattgggt atcaagaggc tggctgctat
     2881 gatatggcgt cttgtttttt aagcgaggaa agattttgag tgcgggacgc ctgaataaaa
     2941 aatctctggg tatcgtgatg tttttatcgg ttggactgct attggcgggc tgttcgggta
     3001 gcaaatcatc cgatacagga acgtattccg gctccgttta caccgtgaaa cggggggata
     3061 cgctatatcg tatttcgcgc accacgggaa ccagcgtaaa agagctggcg cgactgaacg
     3121 gcatttcccc cccttacacc attgaagttg gtcagaaact aaaactgggt ggggcgaaaa
     3181 gtagcagtag tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca tcggttacac
     3241 cgtcatcagc ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt tggttatggc
     3301 caacgacagg gaaagttatc atgccgtatt cgacagcaga tggcggcaat aaagggattg
     3361 atatctcagc tccacggggt acacctattt acgccgcggg tgcaggaaag gtggtgtatg
     3421 tgggcaacca gctgcgtggc tacggtaatc tcatcatgat taaacacagt gaagattaca
     3481 ttacggctta cgcccataat gacacgatgc tggtaaataa tgggcaaagc gtgaaggctg
     3541 ggcaaaaaat cgccactatg gggagcacgg atgcggcatc tgttcgcctg catttccaga
     3601 ttcgttaccg tgcaacggca attgatccgc tacgttactt gccgccgcag ggcagcaagc
     3661 caaaatgctg atggcgaatt aatcagcagt cagcagcgcg gcacttgcta aggagagcgt
     3721 aaggtttata atgccttacg catctcgaag cgggcgtagt ttaatggtag aacgagagct
     3781 tcccaagctc tatacgaggg ttcgattccc ttcgcccgct ccatgacact tcttaaagtt
     3841 caataactta ctgaatcata agggatatgg cttaacgcct tccgatagtt ttactcactg
     3901 taaccaacac tgtgactaac tttgttgaag tagtgagcag acctaaggtc tcagtacatg
     3961 ctcaactcaa gaacctacct ttatcagcgt aatggtgtct tttacatccg tcttaggatg
     4021 aagaccactg gtcgccttac cgcttcgctt ccttctcaca accgttataa actagcgtca
     4081 gtatctttac ggaccaagga tagacgtacc gctatggctc actcccggca catcaagtca
     4141 gcacttaaag caatccatgc agacaaccct aacgcctctt atgaggagct acgggagcac
     4201 ctgaagacca ttgtcgagtg ggaactcagt gtcagccgcg atgacctgaa cgaccctgag
     4261 tcctatcagc tttacgttga ccagtacgac gatattaagt ccaaccttcg ggaagctgta
     4321 gcgactgagc acctgactgt agaccagcat cgctatatca atgacgttat cggtgtgctt
     4381 aaagcctgtc aggatagact caaagg
//
LOCUS       DAJTNM010000209         4394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTNM010000209 DAJTNM010000000
VERSION     DAJTNM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.210"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..809)
                     /gene="rarD"
                     /locus_tag="OO172_004032"
     CDS             complement(<1..809)
                     /gene="rarD"
                     /locus_tag="OO172_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5513982.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADK"
     gene            complement(861..1328)
                     /locus_tag="OO172_004033"
     CDS             complement(861..1328)
                     /locus_tag="OO172_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5513983.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1493..2362
                     /gene="pldA"
                     /locus_tag="OO172_004034"
     CDS             1493..2362
                     /gene="pldA"
                     /locus_tag="OO172_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5513984.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2495..4324
                     /gene="recQ"
                     /locus_tag="OO172_004035"
     CDS             2495..4324
                     /gene="recQ"
                     /locus_tag="OO172_004035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5513985.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLLDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
BASE COUNT         1098 a         1123 c         1161 g         1012 t
ORIGIN      
        1 atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa catcagcgtc
       61 gggccaatgt actgaaaaaa gcctaacgtt gagagacgca ggcgcgtggc agcggcagta
      121 aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa attcagcgac
      181 atcgggtttt gccccatatg gctggttgaa ctgtcggcaa tagcaaacag gtaaatcgcc
      241 gccacgggca gcagccacat ggtttcaatt aacatgccgg tttgtgcttc taccgcaatc
      301 ttcttgcgca ccaggccgta gaaggcaaaa ctaaatgcca gccccagcgc gataataggt
      361 agcgaaccaa aagtccacag ctggaccaac acgccacata tcgccagaat caccgccagc
      421 cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat gttcaccagc
      481 gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac cgcccagata
      541 aacagtagcc agttgccacc aatcagcacg gcagagactg ccagcataaa aattttctgt
      601 ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat cagcaccacc
      661 ataaagaaaa acgaccagat cacgcgatgc gtcaggatct catcggcggg cacgtagtaa
      721 atcagcttga agtatgctgg cgcaataccc caaataaaat aagcggcaag agcgagtaat
      781 acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt ggtaacagca
      841 gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact ggcaatataa
      901 agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc tgcacgcaac
      961 aggctactgg tagcagtaaa acgctcgcct ctgcctgggc gcagataatc aacgcgaaga
     1021 tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat ggtttcgtgg
     1081 cgggttaagg tacttcccac gcacaccaga ccggcggcga catccagcgc cgacgcaatg
     1141 actccgccgt gcaaaatgct ttgcgcccag ttacccacca tcatcggctg atttttaaag
     1201 gccagctgtg cgaactcttt ttcgtaacgc tccagttcca tccccaatgc gcggttaaat
     1261 ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc ggtcagtacg
     1321 gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat gcttctttgt
     1381 tgttggtttc tactttagga agggataact aacggcaatg gagttaagta tgtaaaatag
     1441 cccgcagaaa aacattcacc ttatcaataa ttcgttacgg agaacacgac cgatgcggac
     1501 tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg cacaagaggc
     1561 aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg ccaatatgct
     1621 gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc tcatctacac
     1681 ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg aaaatgcgcg
     1741 taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg ggattttagg
     1801 cccaaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac tgtccaatag
     1861 cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc tcggttttgc
     1921 caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt ataaccacga
     1981 ctctaacggg cgttcagacc cgacctcccg cagctggaac cgcctttata ctcgcctgat
     2041 ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg gtaatactga
     2101 cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg gctatcacct
     2161 cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct acggcggcgc
     2221 ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc aggtttacag
     2281 cggctatgga gaatcgctca tcgactataa cttcaaccag acccgtgtcg gtgtaggggt
     2341 tatgctaaac gacttgtttt gatgaacggt tgagtggttg gcaaatctgg aatccagcat
     2401 ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata
     2461 gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag tgttgaatct
     2521 ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg
     2581 ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggttg ttatgcccac
     2641 tggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg gccttaccgt
     2701 ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactac aggccaacgg
     2761 cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg aagtgatgac
     2821 aggctgccgc accgggcaaa ttcgcttact gtatatcgcc ccggaacgcc tgatgctgga
     2881 taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgtcg atgaagcgca
     2941 ctgtatctcc caatggggcc acgatttccg cccggaatat gccgcgctcg gtcagttgcg
     3001 ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg acaccacgcg
     3061 ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca gcagttttga
     3121 ccgtccgaat attcgctaca tgctgatgga gaaattcaaa ccgctcgatc agttgatgcg
     3181 ctacgtgcag gaacagcgcg gtaagtctgg cattatctac tgcaacagcc gggcgaaagt
     3241 agaagacacc gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct atcatgccgg
     3301 gctggaaaat aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg atctgcaaat
     3361 tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc gcttcgtggt
     3421 ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggtc gcgccgggcg
     3481 tgatggcctg cctgcggaag cgatgctgtt ttacgatccg gctgatatgg cgtggctgcg
     3541 ccgttgtctg gaagagaagc cgcaggggca gttgctggat atcgagcgcc acaaactcaa
     3601 tgcgatgggc gcgtttgccg aagcgcaaac ttgccgtcgt ctggtattgc tgaactattt
     3661 tggcgaaggg cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca
     3721 gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg tgaatcagcg
     3781 gtttgggatg ggttatgtgg tggaagtgat tcgtggtgct aataaccagc gtatccgcga
     3841 ctatggtcat gacaaactga aagtctatgg tatgggccgt gataaaagcc atgaacattg
     3901 ggtgagcgtg atccgccagc tgattcatct cggcctggtg acgcaaaata ttgcccagca
     3961 ttctgcccta caactgacag aggccgcgcg cccggtgctg cgcggcgaat cctctttgca
     4021 acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg
     4081 caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga ttgccgatga
     4141 aagtaatgtc ccgccgtacg tggtgtttaa cgacgcaacc ttgattgaga tggctgaaca
     4201 gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc gcaagctgga
     4261 acgctttggt aaaccgttta tggcgctgat tcgtgcgcat gtcgatggcg acgacgaaga
     4321 gtagtcagca gcataaaaaa gtgccagtat gaagactccg taaacgtttc ccccgcgagt
     4381 caaatgtatg ttga
//
LOCUS       DAJTNM010000210         4381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTNM010000210 DAJTNM010000000
VERSION     DAJTNM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.211"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(64..822)
                     /locus_tag="OO172_004036"
     CDS             complement(64..822)
                     /locus_tag="OO172_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5513986.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO172_004037"
     CDS             complement(830..1933)
                     /locus_tag="OO172_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5513987.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3113)
                     /locus_tag="OO172_004038"
                     /pseudo
     CDS             complement(1943..3113)
                     /locus_tag="OO172_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3181..4206)
                     /locus_tag="OO172_004039"
     CDS             complement(3181..4206)
                     /locus_tag="OO172_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5513988.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1215 a         1148 c         1024 g          994 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 gaattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg caatattacg gaagatctca atataaatcg tggaaagctt tcgtagcagc
     2761 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     2821 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     2881 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     2941 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac
     3001 caaccaacaa tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc
     3061 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3121 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3181 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3241 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3301 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3361 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3421 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3481 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3541 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3601 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3661 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3721 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3781 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     3841 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     3901 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     3961 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4021 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4081 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4141 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4201 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4261 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4321 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg
     4381 a
//
LOCUS       DAJTNM010000211         4069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTNM010000211 DAJTNM010000000
VERSION     DAJTNM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.212"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..352)
                     /locus_tag="OO172_004040"
     CDS             complement(<1..352)
                     /locus_tag="OO172_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5513989.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQ"
     gene            complement(563..1960)
                     /gene="ccp"
                     /locus_tag="OO172_004041"
     CDS             complement(563..1960)
                     /gene="ccp"
                     /locus_tag="OO172_004041"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5513990.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2365..4014
                     /locus_tag="OO172_004042"
     CDS             2365..4014
                     /locus_tag="OO172_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5513991.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDLYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVSREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1052 a         1026 c          949 g         1042 t
ORIGIN      
        1 cctgaccatt tttcggcgca ggcgcatgcc acagatcggc aaccatattt acgcaacgca
       61 ggtcgatggc tgcggattgc ggatattctt ctttgtcgat ccagttttta ttgatcgaca
      121 aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg gctaggttct
      181 gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg acatcatcgc
      241 gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc tttgcgccaa
      301 aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc atttcgaact
      361 ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga agcaaggcat
      421 tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta taatataaac
      481 atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg cccaaacatt
      541 cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg tacaccccgt
      601 tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc tctttgccta
      661 cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca ccgcgatgga
      721 agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc aatttatcac
      781 gctcttcttt agtcacattc atacgaccaa tatccgccgc cgtaatttcc ccaaagttaa
      841 agtctttttt cagcaccaac ggttcaaagg aacgtccgcc gagaataata ccaccatgac
      901 aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc gccgtcagcg
      961 cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt aatgttttct
     1021 caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga taaacttcga
     1081 ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg tcccaggatt
     1141 tcgacgccat ttcaatcggg ttcaacggcg gtccacctgc ctgatcctgc aatgttgccg
     1201 cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc ggcgcattaa
     1261 tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg ccccccgcat
     1321 tcaacgtatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg ggatcgtgat
     1381 acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc gggatgggct
     1441 gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat tccgcgcgct
     1501 gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta cccgcccagt
     1561 gtagcgcggt ataacgcgtt ggtggcatag tttcatactg catcacccat tcaatcttat
     1621 tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg gcctcgaggt
     1681 taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg ccaggaatat
     1741 aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct ttttcgcgga
     1801 gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg gcctgtttac
     1861 tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa atcgcgacgc
     1921 cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc tcacggtggt
     1981 gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg agacaaagcc
     2041 atcataaaaa acagggagat acgctttttg ataggaatca atcaattaca tgaatataaa
     2101 ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc atgattgcgg
     2161 cgagagcagg attgaatgtt ctgaaaatga aaaacaacac caaatccgta tttttataaa
     2221 ttccattgat attagtgcgt tatatttttt gacaagttaa taaacgtgat ccaccgcacg
     2281 ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg ataattagcg
     2341 acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc taatccagac
     2401 gaactgatga tcgaggtcga tctctgctat gaactggacc tgtatgaatt aaaactggat
     2461 gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc ctctgatgcg
     2521 ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc gaaaattttc
     2581 cccgacagta aaacctttcc cgactgcgca cccaaaatgg acccgctgga tattttaatc
     2641 cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt tgaaaatcac
     2701 ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa ttccctgaaa
     2761 gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca cattccgtgg
     2821 tcttctctgc tggcgctacc gcagtcatat attgtcccgg gcggccgttt tagcgaaacc
     2881 tactattggg attcctattt caccatgctg ggactggcgg aaagtggtcg ggaagatttg
     2941 ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actacggtca catccccaac
     3001 ggcaaccgca cctattattt gagccgctcg caaccaccgg tttttgcgct gatggtggag
     3061 ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct taaaatggaa
     3121 tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta tcgccatgtt
     3181 gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga cacgccgcgt
     3241 gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc gcccaacgag
     3301 gtgtaccgcg atttacgcgc gggagcggcc tccggttggg attactcttc ccgttggctg
     3361 cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat cgatctgaat
     3421 gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa aggcgagaaa
     3481 gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt aaaccgttac
     3541 ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga acaactggcg
     3601 ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca tgaacaggcc
     3661 gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat tttggcaagc
     3721 gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt acaatggatg
     3781 gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat cgcgcgcagt
     3841 tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga gaaataccat
     3901 attgccgatg gtgtttcccg cgaaggcggc ggtggcgagt atccgttgca ggatgggttt
     3961 ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc ataatatttt
     4021 tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAJTNM010000212         4038 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTNM010000212 DAJTNM010000000
VERSION     DAJTNM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.214"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            82..285
                     /locus_tag="OO172_004043"
     CDS             82..285
                     /locus_tag="OO172_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCR5513992.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            285..1310
                     /locus_tag="OO172_004044"
     CDS             285..1310
                     /locus_tag="OO172_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5513993.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(1363..1452)
                     /locus_tag="OO172_004045"
     tRNA            complement(1363..1452)
                     /locus_tag="OO172_004045"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1416..1418),aa:Ser,seq:cga)
     gene            1546..2343
                     /gene="mtfA"
                     /locus_tag="OO172_004046"
     CDS             1546..2343
                     /gene="mtfA"
                     /locus_tag="OO172_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5513994.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2444..2519
                     /locus_tag="OO172_004047"
     tRNA            2444..2519
                     /locus_tag="OO172_004047"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2477..2479,aa:Asn,seq:gtt)
     gene            2681..3943
                     /locus_tag="OO172_004048"
     CDS             2681..3943
                     /locus_tag="OO172_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5513995.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1130 a          928 c          959 g         1021 t
ORIGIN      
        1 tcccgagtca ggctgatttt taatgttcaa ccctaattgc cgctaaccgt atatagttag
       61 cggcggttat gagatatagc tatgagcagc ttatttttaa ccgaagatga attgctaata
      121 ttaacgggct gcaaatatgc aagccaccag cgaaaatggt taatggaaaa cgggcttccg
      181 ttctatacca atcgtagtgg caaaccgatt gtcagccggg atctatttac ctgcaataaa
      241 actttaccac cacgcgaggt agagccgaat tttggtgcga tctgatggga agacgaagga
      301 aaaatcctga acacgaaaaa ttacctccaa atgtataccc aaataaatat agttatgtat
      361 ggaaaccaac atccagagaa tctgtaacac taaccgccat caaggatggt ttagctgctt
      421 tatggaaaaa gtatgaggaa actgtaaata atcgcgatcg tgcaatgaca ttcggtcgct
      481 tgtgggaaaa attcctcgcc agcgcctatt acagtgacct cagtccaaga acacaaaaag
      541 attatctgca acatcaaaaa aagttgcttg ccgtattcgg taaggtgccg gcagattcca
      601 taaaaccaga acacatccgt cgatacatgg acaagagagg ggaacagagt aaaacgcaag
      661 ccaaccatga aaaaagcagt atgtcccgcg tttacagttg ggggtatgag cgagggtacg
      721 tgaaggctaa cccatgtgca ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg
      781 taaccgacaa agaataccag gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa
      841 tggaaattgc ctatctgtgt gcagcgaggg tttccgatgt gttatcgctg aaatgggagc
      901 agattggaaa cgacgggatc tttatccagc aagggaaaac aggaaaaaaa cagataaaag
      961 catggagtcc acgattacag gcggcgatcg aaaaagcaaa acagttacca acatccgcct
     1021 atgtaatcag caatcaatac ggcaaccgat atatgtacaa aggctttaac gaaatgtggg
     1081 tagaagcaag aaatcgcgca ggcaaaattt caggtatttt aaccgacttc acctttcatg
     1141 atctgaaggc gaaaggaatt tcagactatg aaggaagcag tcgggataag caacttttct
     1201 ctggtcacaa aaccgagggg caagtgctaa tctatgacag gaaggttaaa gtttcaccga
     1261 cacttgatgt cccgttacct gaaaatattc caagaaaata ttccaagtaa ttccaagtgt
     1321 gatttttgtc actgacttaa tgatgtataa gtgattgaat tttggcggag agagggggat
     1381 ttgaaccccc ggtggagttg cccccactcc ggttttcgag accggtccgt tcagccgctc
     1441 cggcatctct ccgctcagat ggttgccatg atgccaggaa atttggcatt ttaacagtcc
     1501 ctgtccgtgc aattttgttc aagtgacgag tttgcgagca aaacgatgat taagtggccc
     1561 tggaaagtac aagaatcagc acatcaaact gcccttccct ggcaggaagc actatcgatc
     1621 ccccttttaa cgtgtctgac agaacaggaa caaagcaaat tggtcgctct tgccgaacgt
     1681 tttttacagc aaaaacggct tgttccttta cagggctttg agctgaattc attaagaagc
     1741 tgccggatag cacttctatt ttgcctgccc gttctggagt taggactgga atggctggat
     1801 ggttttcatg aagtcttaat ttatcctgcg ccatttgtgg tcgatgatga atgggaagac
     1861 gatatcggtc tggtgcataa ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg
     1921 cctatcgttt tgaactggtt ggatatacaa gattcttttg atgcttctgg ttttaacctg
     1981 attattcatg aagtcgctca taagctggac acccgtaacg gcgatcgcgc cagcggagtt
     2041 ccctttattt cgttgcgtga ggttgctggc tgggaacacg atcttcatgc tgcaatgaac
     2101 aacattcagg aagaaatcga attagttggt gagaatgcgg cgagcattga tgcttatgct
     2161 gccagtgatc ctgctgaatg ttttgccgta ctttctgaat atttctttag cgccccagaa
     2221 ctttttgctc ctcgtttccc ttcattgtgg caacgtttct gtcaatttta tcaacaagat
     2281 cctttgcaga gactgcatca cgctaatgat acagactcgt tttcggcgac gaatgttcat
     2341 taattaacaa ctttgcagat taattaacca attgaaatgg cttatgaaat ttagtgttga
     2401 cagacaaggt accgctaagt aatatgcgcc ccgttctcac gattcctctg tagttcagtc
     2461 ggtagaacgg cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa
     2521 ttttctgttt tcatgcatcc ttgcgaatct ttatctgatg ttgattcaac aggttagtgt
     2581 gaaaactctt ctcggtgcat tttgatttta ccctacgcat cgggaaaaat tggtggtcaa
     2641 atctgggatc aggttagttc gataatggag tgacccccat atatccctta ccgacgcaaa
     2701 aatccgcacc ctcaagcctt ctgataaacc ctttaaagtc tccgattctc acggtctgta
     2761 tctgctggtc aagccgggtg gctcccgcca ctggtatctc aaataccgta ttagcggtaa
     2821 agaatcccgc attgcgctgg gtgcctatcc agccatctcc ctgtctgatg cgcgacagca
     2881 acgtgaaggt atccgtaaaa tgctggcgct gaatatcaac ccggtacagc agcgggctgc
     2941 tgaacgtggc tcacgaacac cggagaaagt ttttaaaaac gtggcgctgg cgtggcataa
     3001 aagtaacagg aaatggtcgc agaacaccgc cgaccgtctg cttgccagcc tgaacaatca
     3061 catctttccg gtcatcggga acctacctgt atcagaactt aaaccccgtc atttcattga
     3121 cctgctgaaa gggatcgagg aaaaaggtct gctggaggtt gcgtcccgca cacggcagca
     3181 cctgagtaac ataatgcgcc atgcggtcca tctggagtta atcgatacga accctgcagc
     3241 aaaccttggc ggcgtgacca cacctcctgt cagacggcac tatcctgccc tgccgctgga
     3301 gcggctgcct gaactgcttg aacgtattgg ggcatatcat cagggccgtg aactgacccg
     3361 gcatgccgtt ctgctgatgc tgcatgtgtt cattcgctcc agtgaactgc gtttcgcccg
     3421 ctggtcagag attgatttca caaaccgagt ctggacgata cccgcgacgc gagaacccat
     3481 tattggcgtg cgttattccg gccgcggggc aaaaatgcga atgccgcata tcgtccccct
     3541 ctcagaacag tccatcgcca ttctgaaaca gattaaggat atcaccggta ataatgaact
     3601 gatcttcccc ggcgaccata acccgtataa gccaatgtgt gaaaacacgg tcaataaggc
     3661 actgcgggtg atgggttacg acacgaaaaa ggatatctgc ggtcacggct tccgggcaat
     3721 ggcatgcagt gcgctgatgg aatcgggttt atgggcaaag gacgcagtag aacgccagat
     3781 gagtcatcag gagcgcaata ccgtgcgcat ggcttatatt cataaggcag agcacctgga
     3841 agcccgcaaa gcgatgatgc agtggtggtc ggattatctg gaagcatgcc gagaatctta
     3901 tgcaccgcct tatacaattg gtaaaaataa gtttatccca tagtatacta acgaatccca
     3961 cactctggca aattaccacc acctctacag gaggtggttc tatatagact tttatcaact
     4021 ttatgacgaa aaacacct
//
LOCUS       DAJTNM010000213         4016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTNM010000213 DAJTNM010000000
VERSION     DAJTNM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.215"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            102..821
                     /locus_tag="OO172_004049"
     CDS             102..821
                     /locus_tag="OO172_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5513996.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            832..2259
                     /locus_tag="OO172_004050"
     CDS             832..2259
                     /locus_tag="OO172_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5513997.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2252..2947
                     /locus_tag="OO172_004051"
     CDS             2252..2947
                     /locus_tag="OO172_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5513998.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(3190..3859)
                     /locus_tag="OO172_004052"
                     /pseudo
     CDS             complement(3190..3859)
                     /locus_tag="OO172_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1067 a          844 c         1048 g         1057 t
ORIGIN      
        1 gctagccaat ttaaaaagaa cgccatattt attgatgatt gatcccccgg tgggggggaa
       61 acatcgggca taaatgggca tgaagtaatg gagtattagt tgtgaatgtc aatttctttg
      121 tcacctgtat tggtgacgcc ctgaaatcaa gaatggcacg agactccgtg ctgctactgg
      181 aaaaactcgg ctgtcgcgta aatttcccgg agaaacaggg atgctgcggt cagcctgcga
      241 tcaatagcgg ttatatcaaa gaagcgattc cagggatgaa aaatctgatc gccgcactgg
      301 aggataacga cgatcccatt atttcaccgg ctggctcttg cacctatgcc gtaaaaagtt
      361 acccgacgta tctggcggat gaacctgaat gggcatcacg tgccgaaaag gttgccgcgc
      421 gtatgcagga tctcacctct tttattgtta ataaattagg ggtagtcgat gtaggtgcca
      481 gtttgcaagg gagagctgtg tatcacccat cttgtagcct ggcccgtaag ctgggagtga
      541 aggacgagcc acttacgctg ctgaaaaatg tgcgtggact ggagctgttg acctttgctg
      601 aacaggatac ctgctgcgga tttggcggca cgttctcggt caaaatggcc gaaatatccg
      661 gcgagatggt gaaagaaaag gttgtgcacc tgatggaagt ccgccctgag tatttaattg
      721 gtgctgacgt gagttgcctg ctgaacatca gtgggcgatt acaacgggaa gggcagaaag
      781 tcaaagtgat gcatattgct gaagtgttga tgagccgctg aggatataaa gatgtcgatc
      841 aaaaccagta atacagattt taagacacgc atccgtcagc aaattgaaga tccgatcatg
      901 cgcaaagcgg tggcaaacgc gcagcagcgt attggggcaa atcggcaaaa aatggtcgat
      961 gaattggggc actgggagga gtggcgcgat cgggccgccc agatacgtga tcatgttttg
     1021 agtaatctcg acgcttatct gtaccagctc tcagaaaaag tgacgcaaaa cggcggtcac
     1081 gtctattttg caaaaaccaa agaagacgct acccgctaca ttttacaggt tgcccaacgc
     1141 aaaaatgccc ggaaggtggt gaaatctaaa tcgatggtga ccgaagagat tggtgtcaat
     1201 catgtgttgc aggatgctgg cattcaggtg attgaaaccg atctgggtga atatattctc
     1261 cagctggatc aagatccgcc atctcatgtt gtggtcccgg caattcataa agatcgccat
     1321 cagatccgtc gagtgctaca cgaacgtctg ggctatgagg ggccggaaac gcctgaagcg
     1381 atgaccttat tcatccggca aaaaatccgt gaagatttcc tcagtgctga aataggtatt
     1441 accggctgta atttcgcggt ggcagagacc ggttcggtat gcctggtgac caatgaaggt
     1501 aatgcgcgaa tgtgtaccac gctgcctaaa acgcatattg cagtgatggg aatggagcgt
     1561 attgccccca cgtttgccga ggtagatgta ttgatcacca tgctggcgcg cagtgccgtt
     1621 ggtgcacgtt tgacgggata caacacctgg ctgacagggc cgcgcgaagc ggggcacgtt
     1681 gatggtcctg aagagtttca tctggttatt gtcgataacg ggcgttctga ggtgctggcc
     1741 tctgaatttc gggatgtgct gcgctgtatt cgctgcgggg cttgtatgaa tacttgtccg
     1801 gcatatcgcc atattggcgg tcatggatat ggctctattt atccagggcc aattggtgcg
     1861 gtgatttctc cgctacttgg cggctataaa gattttaaag atttacccta cgcctgctct
     1921 ttatgcactg cttgtgacag cgtgtgtccg gtgcgtattc cgctgtcaaa actgattttg
     1981 cgtcatcgtc gggtgatggc tgaaaaaggg atcaccgcaa aagcagagca acgggcgata
     2041 aaaatgttcg cttatgccaa tagtcatcca ggattgtgga aagtcgggat gatggccggc
     2101 gctcatgcgg caagctggtt tatcaatggc ggtaaaacac cactcaaatt tggcgcgatt
     2161 agcgactgga tggaagcacg cgatcttcct gaagctgacg gagagagttt ccgtagttgg
     2221 tttaagaaac atcaggcgca ggagaaaaag aatggataat cggagcgaat ttttgaataa
     2281 cgttgctcag gcactgggtc gcccgctgcg acttgaaccg caagcagaag atgcgccgct
     2341 taacaactat gctaacgagc ggcttaccca acttaaccaa cagcagcgct gtgacgcgtt
     2401 tattcagttt gccagcgatg ttatgttgac gcgctgtgag ctgaccagcg aggcgaaggc
     2461 ggcagaagct gcaatacgtc tgtgtaaaga gctgggagat cagtcggtcg tgattagcgg
     2521 tgacacgagg ctggaggaat tggggattag tgaacgtttg cagcaggaat gcaatgccgt
     2581 tgtttgggat ccggcgaaag gtgccgagaa tatctcgcag gcagagcagg ctaaagtggg
     2641 tgtcgtgtat gctgaatatg gtttaaccga atcgggaggc gtggttcttt tttccgccgc
     2701 cgagcgcggg cgttcattga gcctgctccc ggaatcttct ctttttatcc tgcgtaaaag
     2761 cacaatcctg ccgcgtgtag cgcaactcgc agaaaaattg catcagaaag cgcaggccgg
     2821 tgaacgaatg ccgtcatgta ttaacatcat cagtggtccc agctcaacgg cggatattga
     2881 gcttatcaaa gtcgtcgggg ttcatggtcc ggtgaaagcg gtgtatctga ttattgagga
     2941 ttgctgagag agatatgaga tctgaacata aacctgaaga aggcatacga ggttgaaaat
     3001 agcgttaagg acaacctgac ttaaccccgt tccgtcatgg ttatgctgct cttctgtaaa
     3061 tatccaggca ttagtaatgc caggatattt acagtgaaaa agacaggcat ccattcctga
     3121 aaacgggttc ccttcccggt cattgtcact tcagctttat acaggcactc tctatcagaa
     3181 tgctgtttat tactgcccag agttttgttg gtattcatct accatttttt tgacaaggca
     3241 aaacattaca gaaattacaa tgcttagaaa aaaatcccag agctaggcaa atggcagcag
     3301 ggtatttatt ttttatttct ggtgagctta tagttacaca gccatcaccg ttcggataca
     3361 atgaccagtt cctggtcagg tatatcattt ttgtataaag ctcactgtta attgctgaag
     3421 gcgtttgtat ctcatcgaga acttgcctgc cattatggta ttctgacgca taccatttga
     3481 taatagacgc ctgcacgcga gatatttcct cttcagcagt tggtaaatct ctcttatctc
     3541 ctgaccattc cagccttatt tctctgttct tgatatctgg gctggtctga aaaaatgtag
     3601 taattcgctg aataacatct tcagtcattg tggtctgaca ggcaaaatta ttattgctat
     3661 cacagctaac aagtatacct ggcgttgtct ggtttatttt aaggctgtaa atcacttttg
     3721 tattgtcttt attgatatac agaaacaaca gcaccattgc catacagata gccataataa
     3781 aaaggataac acgaatcttt ttcttcagat gaaataaaat ctcaatcaga tcgatatcct
     3841 gtttgatttg ttcactcata atatatctaa aatctgtcga agataatgtt gtattaatag
     3901 tctgtatcag gaatgttcgg gttaaatatc agcaaaaaac cagcatcatg aatactggat
     3961 atgaagcatg agagttacct cagtgtttta tataacgatt cggtccccat atctgg
//
LOCUS       DAJTNM010000214         3784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTNM010000214 DAJTNM010000000
VERSION     DAJTNM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.216"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..224
                     /locus_tag="OO172_004053"
     CDS             <1..224
                     /locus_tag="OO172_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5513999.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="OO172_004054"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="OO172_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5514000.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="OO172_004055"
     CDS             518..1108
                     /locus_tag="OO172_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5514001.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="OO172_004056"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="OO172_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5514002.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="OO172_004057"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="OO172_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5514003.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="OO172_004058"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="OO172_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5514004.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3456..3647
                     /locus_tag="OO172_004059"
     CDS             3456..3647
                     /locus_tag="OO172_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514005.1"
                     /translation="MTENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1033 a          924 c          946 g          881 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcaa cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtcgcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc tggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccgtaaggtt acaaccgttc acgtcataaa gcgccagccg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtg tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacagatat
     3121 gcacctccca ccgcaagcgg cgggccccaa ccgaagccgg tttagttaca acatgcaaac
     3181 ataaacccct aaaaaaccca agtccaacgc agaactgaaa ccacaacgcc cctcctcata
     3241 actgaaaagc gccgccgccc cgcccgaagg gcgggaacag agtcgctttt aattatgaat
     3301 gttgtaacta catcatcatc gctgtcagtc ttctggctgg acgtatcgag tacacgctcg
     3361 taagcggccc tcacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaaccctcg
     3421 tcgggaccac tccgaccgcg cacagaagct ctatcatgac tgaaaacggg tatggcttag
     3481 cagggctggg gatgggtaag gtgaaatctg tcaatcagta ccggctgacg ccgggcttcg
     3541 gcggttttac tccggtatca catgtaacca cagcgtgccg ccttccatgc cgctggcgcg
     3601 gcatcagaat aatacaagca gcatttaatg catttgcaaa agtgtgagta atattaagtg
     3661 gtcgtttcag ggatatcgtt aagacttgag cgataggaat taaaacccca aaaagattaa
     3721 aaaaacacca caaaacggat gtttcttcaa caccactttt gctccatatg aacggaaccg
     3781 acga
//
LOCUS       DAJTNM010000215         3726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTNM010000215 DAJTNM010000000
VERSION     DAJTNM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.217"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            180..437
                     /locus_tag="OO172_004060"
                     /pseudo
     CDS             180..437
                     /locus_tag="OO172_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO172_004061"
     CDS             complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO172_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5514006.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHKQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO172_004062"
     CDS             complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO172_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5514007.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO172_004063"
     CDS             complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO172_004063"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5514008.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3062..3205)
                     /locus_tag="OO172_004064"
                     /pseudo
     CDS             complement(3062..3205)
                     /locus_tag="OO172_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3595..>3726)
                     /locus_tag="OO172_004065"
     CDS             complement(3595..>3726)
                     /locus_tag="OO172_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5514009.1"
                     /translation="AHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
BASE COUNT          963 a          884 c          888 g          991 t
ORIGIN      
        1 cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat tagctaacta
       61 atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag acaacggtga
      121 aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag gattacatca
      181 tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg
      241 ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt atagaacagt
      301 tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca tcgatgattt
      361 ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt tatttccttg
      421 gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc agttattgaa
      481 gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact ggtcaggttt
      541 aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa
      601 tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg cctgtaaatg
      661 ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg tcatgttttc
      721 attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc cacgaatggc
      781 atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa ttggcgttac
      841 cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca gttcgatcgt
      901 ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg cgagattcac
      961 cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc acaccgggct
     1021 tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc
     1081 atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt cctgaatgat
     1141 ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc cagcattgag
     1201 ataatatttt gcctgcttgt ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat
     1261 agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt catagatcgg
     1321 caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc gccgcaatgc
     1381 tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca tagccataca
     1441 gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc catatctggt
     1501 gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt aatgatatga
     1561 cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga ccagcaagct
     1621 gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc agcataccgc
     1681 ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc agatagttac
     1741 caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg cgttgggcaa
     1801 aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt ttcttcgaca
     1861 acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc atcatctgct
     1921 gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga atttcacata
     1981 ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg atggcgtcaa
     2041 atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga
     2101 ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt tcatcaccag
     2161 ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata ttatcaagct
     2221 gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct
     2281 ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt tccgggatca
     2341 taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta gggagcaagt
     2401 aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata tatgtctatc
     2461 tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa caagctggca
     2521 caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc actcttttcg
     2581 ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc gatggcaagg
     2641 tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg
     2701 gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg ggatagtgcg
     2761 tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg gaagcgtatc
     2821 aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac cacctgggtt
     2881 ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca gctgcaccat
     2941 gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat cgcaaatcac
     3001 ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca tccgttctct
     3061 cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga taccacgcca
     3121 gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact aacgaatagt
     3181 aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga ttttttaatt
     3241 tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga taattccagt
     3301 cagtgaaatt ttagcgtagg ctatctgtta tttgattctg tggcgtgata gccgccagac
     3361 accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt tacagagcta
     3421 aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt acacacagca
     3481 gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt gttataacgg
     3541 ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata taagttaaat
     3601 catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt taccgggcga
     3661 gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat agcccagacg
     3721 atgggc
//
LOCUS       DAJTNM010000216         3722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTNM010000216 DAJTNM010000000
VERSION     DAJTNM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.218"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..737
                     /locus_tag="OO172_004066"
     CDS             <1..737
                     /locus_tag="OO172_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5514010.1"
                     /translation="ATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCEEINR
                     LNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTNFVF
                     SGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQELCGV
                     DLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERVMDRM
                     VMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            752..1174
                     /locus_tag="OO172_004067"
     CDS             752..1174
                     /locus_tag="OO172_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5514011.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1232..1588
                     /locus_tag="OO172_004068"
     CDS             1232..1588
                     /locus_tag="OO172_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514012.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            1681..1899
                     /locus_tag="OO172_004069"
     CDS             1681..1899
                     /locus_tag="OO172_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5514013.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            1901..2263
                     /locus_tag="OO172_004070"
                     /pseudo
     CDS             1901..2263
                     /locus_tag="OO172_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            2263..2499
                     /locus_tag="OO172_004071"
                     /pseudo
     CDS             2263..2499
                     /locus_tag="OO172_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2692..2928
                     /locus_tag="OO172_004072"
                     /pseudo
     CDS             2692..2928
                     /locus_tag="OO172_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2928..3293
                     /locus_tag="OO172_004073"
     CDS             2928..3293
                     /locus_tag="OO172_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5514014.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT         1013 a          780 c         1038 g          891 t
ORIGIN      
        1 ttgcgacagg aggcgttctg gagcgtatcc gcagactgac cccaccacat gtaaccgccc
       61 cattcagaac ggttgcggag tggcgcgagt ggcaacttgc tgaaggccag aaacgttgcg
      121 aggagatcaa ccgcctgaat cgtcagttgc gggtggaaaa aattctgaac cgctccggca
      181 tccagccgtt gcaccggaag tgttcgtttg cgaattaccg ggcgcagaac gacggtcagc
      241 gacatgcact gagtcaggcg aaatccatag ctgacgaact gatgacaggc tgtacgaatt
      301 ttgtgttcag cggtaagccc ggcaccggaa aaaatcatct tgcagccgcg attggcaacc
      361 ggctgatggc gaaggggcgt agcgtgatta tcgtcaccgt gtccgatgtc atgagcgtgt
      421 tgcatgagag ctacgacaac ggcaaatccg gtgaaaaatt tttacaggag ctttgtggtg
      481 ttgacctgct ggtcctggat gaaattggca tgcagcggga tacgaaaaac gagcaggtgg
      541 tactgaacca gattgttgat cgccggacgg catcgttacg cggtgtgggg atgctgacaa
      601 atattaacca tgcagcgatg aatacacttc tcggcgagcg ggtgatggat cgcatggtca
      661 tgaacggcgg gcgctgggtg aattttaact gggagagctg gcgtccgaat gttagccatt
      721 cgagggttgt taagtagttt caggaggatt tatggcgaaa ccttttactc ccgaacagcg
      781 ggaagaactg aagacgcgaa ttgtggaact cgtgcatcag gacggtcggg tcacgattcg
      841 gcagttgtca gatgaaacag gtatcagtcg tgcgtctgtc ggtcgcttat gcatggaact
      901 ggtcgcaagt ggtgatgtat ataattctgg ctacggggta tttccctctg aacaggctca
      961 taaagactgg caaaacgccc gcaaaaagct atcgagggta aaggtgaaga aaccggttgt
     1021 ggttgatcct gaccttatct ggtcattacc agacggagaa atacgccgct acgacaggcg
     1081 cctgaatata atctgtcgcg agtgccggaa gagcgaagct atgcagcgcg tgctggcgtt
     1141 ttatcagggg aaatttcagg aggcggtact gtgagtaaaa ttagctatca ggcttcaatt
     1201 accgctggta ttcgcatcaa aggagaggag catggaaata aaaccagaag atgagttaag
     1261 caatatcgtt ttatttccgg taaaagagga tgacccacgt aatcaggtta attttcttta
     1321 tgaaccatcg gaaagaccat attgccatca cgcctctgtc cgggttgacg aaaaagagcg
     1381 tcaggtccgc tgtaaaatct gcggtgcggt tgtggagccg tttgactgga tgctctctgt
     1441 ggcaaaaaga gaaaccagac tggcagatga tgtaaggcta ttgcgccagg aggaacagga
     1501 aaggcggaga aatatagaaa agctgataca gattgagcgt aacgcgaaag cgcggatacg
     1561 cagggcgaca aaatccagaa ctgaataatt aaatttagca ctgttaaaaa tttaatcctt
     1621 aaccggaggg atttctgcac cctcaaatca tcaggagacc gcccgaaagg gcggtaatga
     1681 atggttacat tatttagaaa aaaatatccg cgaaagagta gaacaacaga attcctgttt
     1741 ctcattctgt ttatcgtgtt gatgacaccg atatccccgc taatttttgt ctggataatc
     1801 ggaaaaataa ttgagccagt tattgaattg tataacgacg tggtatgggc gtcattcaac
     1861 acactgcaca ataaaattaa tccgtataag gaaagctgat atggcactga cgaaaaaaca
     1921 acgtgcagaa ctgcgcatga agttcggtgg tcgctgcgct tattgcggct gcgaacttgg
     1981 cgaaaagtgg catgcagacc atgtaaaacc ggtcattcgt tttgatggaa atatgcttca
     2041 ccaggaacgt gacgatatat ccaacatggt accagaatgc cacccatgca atctgcacaa
     2101 gcattgcagt agcctagaag attatcgacg aattatcagt gatggtcgtc gtgaattcct
     2161 tgcgtccggg aaaggcaaag cgctggttcg tatgggattg gttgaaatga aatctgaccc
     2221 ggttgtgttc tggtttgaaa aatatcaaga aggggctacg gcatggccac tattaccaaa
     2281 gagcgactgc aatggctggc taacatttct ggccgcgatg atattgacga tatagacggc
     2341 ggtgaaattc gtgaactggc gcttatcgct ctggcatcac tggaagcaga gcctgtagcg
     2401 gaatgcattg ttgaagatgg gggcatgtgt attgacgggt tcggtgagta tgtgggtcac
     2461 tcgctgcctg atggaatgca tgagctttat gctgccccgc gaatgcgaca acctgatgga
     2521 tggaaagccg tagctgtagc gtggaaggtg acgtttactc aagttgacca ggaatctaat
     2581 acgttcactg ctatatattt tgacaaagcg gaagttgagc gttgggtacg actgcataaa
     2641 gcatgtgatt ttcgggcaga tataacaccg ctttacgcag cgccggcagt gccggttgca
     2701 gtaaacgacg acatggctta cgcattccat cacgcactgt cagattcatc gcttggctct
     2761 ggtgaaatcg aagaaattaa aaccggtttg cgtgctgcct ttgccaacgt cactatccaa
     2821 ccagagccga tagtgccgga tgaaatcggg ccaaacgata gtaatacgtt tgattatgtt
     2881 gatggctgga acgcctgccg cgctgccatg cttaagggag ataaataatg attaatcgaa
     2941 ccaaactgga gcacattctc gaatatgcca ggcagcagaa atgtattggg caactttgta
     3001 aaattccacc aggagatatg gttgaaatcg tggaaatggc catgcgtaag gctggcaact
     3061 ctccggtaac tctggctggc tggataagct gtagtgatgc agttcctgct gaatattgcg
     3121 atgtgattct tctcgatgat ctcgggaatg tattccccgg ttcctgggat aaggtttttt
     3181 gccccactcg tggcgggaat aagatggctt ttgtggacaa agacggcgtt gaagtagaga
     3241 gctcaactca ctggatgccg ctaccggaac caccgcagga ggtgaatcaa tgacctggcc
     3301 tgaggcattc accacggtag gaattgcgat ggcggtggcg ctggtggtgt attcgatttg
     3361 ccgttggggt taacaaacaa aaaccccgga ttgatggtcc ggggtttttg aaggaaacaa
     3421 aacagaaaca gcaattgccg ttacctgttg ttaccatggc aagtaaacgt atctcaggcg
     3481 agcgcattgc gccgttctga cgcagataaa ttagcctgga tagaaggtgc tggcaataaa
     3541 aaatagcgtt ttcttatcgg tatcggtaag attgctgcgg gtgcttgagg ctgtctgcct
     3601 cgggcatgcc actgtaaggc agacagagaa aagccccagt taacattacg cgtcctgcaa
     3661 gacgcttaac attaatctga ggcccaatct atgtctcaca aatgtaggtt agcctcttac
     3721 gt
//
LOCUS       DAJTNM010000217         3699 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTNM010000217 DAJTNM010000000
VERSION     DAJTNM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.219"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            913..1353
                     /locus_tag="OO172_004074"
     CDS             913..1353
                     /locus_tag="OO172_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514015.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(1432..3129)
                     /gene="treA"
                     /locus_tag="OO172_004075"
     CDS             complement(1432..3129)
                     /gene="treA"
                     /locus_tag="OO172_004075"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5514016.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(3449..>3699)
                     /locus_tag="OO172_004076"
     CDS             complement(3449..>3699)
                     /locus_tag="OO172_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5514017.1"
                     /translation="PQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALI
                     ARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT          951 a          828 c          895 g         1025 t
ORIGIN      
        1 gtggctaata ttatttaaat catttaaggc taaatcattt aaatatgaat gacattattg
       61 ttttttatcg tccacggtta cgcgacgttg aagttacttg ttttacaatg agcgattttt
      121 gatatctcgc cgagaaaatt atcagcattc tctcgtttca gtgtcgtgaa tatactgtta
      181 acggaggtga aaagaatgct gggggttata caaaacctat gttgttgtcg tccggattca
      241 ccgcttgctg atagtaagca gcgtaaccag aagttaccgc aacaagactt tgaggatttt
      301 aagaacgacg ttattaatgt cattaacaac aatgaagata tttattttct tagagagagt
      361 aatttactga atgcgtttat tcgaacgtgc aaaaaaagag atgataacag ttacggaaat
      421 atgatattta cgctttattt tgataacgat atctatggga acagaattat caaagcatcg
      481 ctgaacggcg gagagttgca gttattaagc acagggtgta gcaatgataa cattctccat
      541 tttattaatg catttctcat tcgggtgaaa tttccgctac ctggacgcat aaaaccggaa
      601 ctctcgccaa aagccgggca ctccgaagga attgacgcgt tgcgctgctc tgtaacggca
      661 tttaccgcaa gccatttatt tcgccagcga caatccgcag agaaccaaac gcatcaggaa
      721 acgactgaac acaataaata ctgtattgat caacatatta tactggcacc tccagcacgc
      781 agtgaggata cactgtatca tcagcgaatc ttatcactgc ggagctggtt gaacgacttt
      841 ctgagcgaaa taagtcggtt cccggtaaca acaacagaat ccttttttaa ttattgtttc
      901 gttgtttttg gtgtgattca gaaaataaat attttagaca gtagccagtc aggcgttaaa
      961 aatggggcgg atagtgaggc ggttttggct gcgcttattg aacatttaga attgatcaat
     1021 ccttctggca gattaagcca aaatacccgt tcggcaatgc ttcagttacg cgaagaggaa
     1081 tggtctgaat tctttttttg gttgctaaat tctctggagt gtctggatta tgtgattatt
     1141 aatttaactc cagaaagcaa aaaaacgttg atgagtgaac accgtaacaa tattcaggta
     1201 gcaattgatg ctttatatag ccaacgtcgg cgtaaatcgc cgggtgatga aagtgaaaca
     1261 cttacccgta gaaatgacgc tatcttcggc aaccacgtat ggcaaacatt tgcgcaatac
     1321 ttcccgccag gacttgaaaa accgtctgtg tagaaaaaac catagatatg gtgttatctg
     1381 ccgacaacgc catccggaga agccgaatga cgttgtcgga gtaagcgctg gttaaggtgt
     1441 gggttgtgcc tctttggttg agggttgcgt cgttgctgac ttaacggtcg gacgcgtcgc
     1501 cggaacattg tcacacggtt gctctttcgg gcagatcaaa tccagcattt tcagcgtcac
     1561 gccattggtc cagccaaagc catcctgtaa tggatattcg ccaccgccgc cccccgttcc
     1621 ggtggtgctg acatcatatt tttccaccag ctttttctcc cggtcatagg tgtgctgaac
     1681 attggtcagg aagtgccagc taatgtccat cgccacctct ttttgcccgt agttttgtaa
     1741 tccttctgtc gcgacccact gtaacggtgc ccagccattt ggcgcatccc attgttgccc
     1801 acttttcacc gacgtggtgt tcaggccgcc gggttgcagc agatgtgttt tcgtcgccgt
     1861 cgccattttg ttggcgcgat ctttcgctgc cgcattgacg tacagcggga acagggcggc
     1921 cgcggttaac tgattgcgca ctttatgact tttcaggtcg taatcggcat accagccttg
     1981 ttgatcgttc cacaggtatt tttcgatccc tttttgacgg gcattcgcca gcgtttcgta
     2041 ctggtttgcc atcgcgttat ctcctgcagc tttgctggcg cgggcgagga ttttttccat
     2101 tttaaacatc aggctgttca gatcgaccgg tacgatgctg gtggtgcgta aggtatttaa
     2161 ctgctgcggg ttgtccatcc agcgcgagct gaaatcccag ccagacgcag cggcagagcg
     2221 caggtcgcgg taaatttcag tggcaggtcg attcggattg cttttggcgg tggcaatatc
     2281 ttccacccat gactctggtc gtggcgtatc gcgatcgtcc cagtagcggt tgagaagggt
     2341 accatcctga agttttacaa cgcgtttttc ctgttgtccg gcttgcaggt tttcaacacc
     2401 gtccatccag taagcatatt ctttttgcat ttgcggcagg tattgcttca acgcggcatc
     2461 gccttcatgc tgcgccagta actctaccat cagggcaaag aagggcggtt gcgagcggct
     2521 taaatagtaa ctgcggttgc cgttgggaat atgaccgtaa gtgtctattt catgagcaaa
     2581 attggccacc atatccgcga ctttatccca gtgaccgctt tcggcaagtc ctaacatggt
     2641 gaagtaactg tcccagtaat atacctcgcg aaaacgtccg cccggcacga cataaggttc
     2701 cggcagcggt aacagagaat cccatttttc ggtgttttcg gtagaacgcg ttaataccgg
     2761 ccaaagtccg tcaatatgtt cgcgcagtga ctgcccctct ggcggaacat atttctcgcc
     2821 ttctttcggc agggtgaaat tgacgttaac gaaatggcgc agatcaaatc cgctctggtt
     2881 ttgctgcatc cgataatcag caaggatcat cagcggatcg ctgttcggca cggcatcggc
     2941 aaaggttttt tggtccggaa aaagtttggc gttttgcaca tcattaaaca gcggccctaa
     3001 taaaatatca ggcggctgtg gtgttaccgg tgtttcttct gcctgcaccg atagcgcagc
     3061 gaaacacaaa aagatacagg ctggaattaa cgccattttt tgcgggcgag aaggtgcggg
     3121 ggatttcatc aatcattctc ctttggcgaa accgaataaa acggtattca agtatcagaa
     3181 aaccttagtt caggatcgca ctagctgcat gatcgaaatc tcattctgcg aacggtcagg
     3241 cattttagaa aagggaatta aagggataaa taacggcgaa atagagtgat caaataacgt
     3301 ctgtttatta gccagccagc gacatttggt tcgcgattag cgaagaaaaa tcatcataaa
     3361 ttggtaaaaa atataacagg tagttaaaaa acatcagtgc tgggtaaatt gccggatgac
     3421 atcagaacga tgccatccga acggtggctt aaccctgacg gttgaaacgt tgcgttttaa
     3481 cgtccagcgt tagcgtttct tctggttgta tcgcatacag tttctcaccc tgctggcaaa
     3541 tccagccaat ccccagttca cgggcgatta gggcgctgtg ggataccgga cttccggcgc
     3601 taaggcagat accttttaca accgccggat ccagttgcag tactgtggaa ggataaatgt
     3661 tctccgccag tagaatagtt ggcgagttaa actgcggga
//
LOCUS       DAJTNM010000218         3660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTNM010000218 DAJTNM010000000
VERSION     DAJTNM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.220"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            33..140
                     /gene="ldrD"
                     /locus_tag="OO172_004077"
     CDS             33..140
                     /gene="ldrD"
                     /locus_tag="OO172_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR5514018.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            516..623
                     /gene="ldrD"
                     /locus_tag="OO172_004078"
     CDS             516..623
                     /gene="ldrD"
                     /locus_tag="OO172_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR5514019.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(710..2389)
                     /gene="bcsG"
                     /locus_tag="OO172_004079"
     CDS             complement(710..2389)
                     /gene="bcsG"
                     /locus_tag="OO172_004079"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5514020.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2386..2577)
                     /gene="bcsF"
                     /locus_tag="OO172_004080"
     CDS             complement(2386..2577)
                     /gene="bcsF"
                     /locus_tag="OO172_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5514021.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2574..3660)
                     /gene="bcsE"
                     /locus_tag="OO172_004081"
                     /pseudo
     CDS             complement(2574..3660)
                     /gene="bcsE"
                     /locus_tag="OO172_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
BASE COUNT          916 a          930 c          985 g          829 t
ORIGIN      
        1 cgaaacgttg tcattacaac acaggcggct atatgacgtt cgcagaactg ggcatggcct
       61 tctggcatga tttagcggct ccggtcattg ctggcattct tgccagtatg atcgtgaact
      121 ggctgaacaa gcggaagtaa cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc
      181 gcccggacca ggccgcaggg gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga
      241 ttgcacgcgg ataatattct gcggaatgcg cagcacagag tgaagacgtg acggattccg
      301 gaacagaaaa acagtcgtag aatcagcggc aagccggaaa ggttccggtg aggcgcaatg
      361 ttgcgggggc tttatccctg gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg
      421 caggtatgca cctgacaaca tcacgggggc taatcttgaa cccaaacaac tcaagaatag
      481 ccgcgaaacg ttggcattac aatacaggcg gctatatgac gttcgcagaa ctgggcatgg
      541 ccttctggca tgatttagcg gctccggtca ttgctggcat tcttgccagt atgatcgtga
      601 actggctgaa taagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt
      661 tgcgcccgga tcaggccgca gaggttaaac tctgcggcct ttttcgtgct tactgcgggt
      721 aaggcaccca gtcgccgccg ttcaggcgaa cgtacggttt atcttggtat tgaataacta
      781 ctgcatttga gttctcggag accggtgctg tttgtggcaa cccactggtg agttttttcc
      841 agtcaacatt gtcttcggtg aaaatcttgc catcaagaac gcgaaccacc agatcggaga
      901 tagccaggaa gctgctcggt tggtcaatga caatcggtgc cccctgatgt ggtgccttca
      961 tgccgaagaa tttcacccca acggggacgt ctgtgataga cgggctaggg atatcacgta
     1021 ggccagatac ctgcattctg tcgcccttca gcgcgccgcc gtgttccggc accacgacca
     1081 ccatcacttt acgacccgat ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga
     1141 atttctgcgc ccgcgctttg taatccgctg ttttgctgac ccccggataa tggttgccgt
     1201 catgcagtgg aagcgtgttg tagaacgtgg cactacggct gtttttatct ttttcggtaa
     1261 cgtccagcca gcggttaagc acggcggtat cgtcataaac cggcgaacca tcaaagccca
     1321 gcaaaataac cggcagattt gtttgatcca tcaattcagt ctgcatgccg ccattttcgc
     1381 gaacttcttt caaaaaaccg ccgaactggc cgttatgtcc catcatcagg tgctgggtaa
     1441 agcccagttt cgaaaggtta tcaaacagat agcagtcgtt atttgccggt tgatacagat
     1501 tagtgtgcga agtctgcccg cagctggcgc gcagtaaacg gatcgccgcc gggccactgt
     1561 aggaggtggc ggagttaaag ttcttgaact caatatcgaa atgcgaccac agtggatgcg
     1621 acatcaaccc ggcggcttct atatccgacc aggaaagcga acagatgtta atcaccagta
     1681 gttcaaatgg ctgagcatca gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct
     1741 ccgcgttata gaaattattc agccaggcgt taaggttcgc cgttgttggc ggtgcagttt
     1801 gtgcgggcat atcacccact accggtgcgc cacccgtcgc cgcaacggtt gccgctgcgt
     1861 taccacccgt cgttgttaca gtggtcgtcg gttgtccggc tggccacaag gagaaacttg
     1921 gtcccgccag ggtaagtacg ttcagccata gcagtatggc aaccacaaaa acggtaatgc
     1981 gaatccattg tgacaggaat aaccaggcca ctaataaaac aaaaatggcc ccaatcatct
     2041 gccagttaat aaagcgtgtg acaaggtcga ttaaataatc ggtactgaac cccgccacct
     2101 gcgaaccctg gctcattatg ctttccgggc caagcaacca ggtgtcatgc cagaacaaag
     2161 caaagccgat cggcagggca atccagtggc gcaagcgatg caggctgtaa cgcggaatgg
     2221 gcatcagcag aaacgcggca aacaccaaat tgaggagcgg gtggaagtta agatatcccg
     2281 cccacaacag gccaaactta accagaaaat agaagttcca gccggaaagg ccgcgccagt
     2341 attgccagag ggaagaaggc atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc
     2401 cctggctttt tccgtgcggc gtaacgtccc ggccggttta acataacgag gtttagcaaa
     2461 gaacaaacgt aaggtgtcgc gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg
     2521 gaaaaatatc agtgcgcaaa cgataataat ttcaatgata tcgctgatgg tcatcatgat
     2581 gagcgctcca cagcatcatc taacagtcgc atgggttctg gtattcgtcg ccagtggcga
     2641 ccatcgtgct cggcattgat gaccggttta gaactttgcg ttaaaggcag cggcattccc
     2701 cattgttctg gggcaagcag gcgcatctgc accagctcgg cactgatttg atcatcttca
     2761 aaccagacca tacggtttga gaaaatgtcg ccagtaggca atgggaaaat atgattcaac
     2821 gcggtatcca gatcgttaat ccgacagaat gagagaaaca gcaccagccg attaccgcca
     2881 atggtcatga tatcgccggt acggttaggg cgacatagcg tcagggcttg ctcaacgcgg
     2941 atacccggta ccggacgtag ggcaaccaga acgcctttac cgtgggcagg caagagaggg
     3001 ttattcatca tgttgttgac ggcattacag aacacatccc acttctggaa accacgcagt
     3061 ttgagcggct gggtcattga cagcaaggta gtgatatctt ccggtacata gcgactaaac
     3121 ttctgccctt acacgctttc gatcatcgtc agacaacggg agagtggcgc attccacgga
     3181 ataaccatat ttgcaccgca ggccaataac aaacgttcat cggtggcgcg caggctagcg
     3241 gtattttccc gcacgaggat tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg
     3301 ctgcgggcca gtggctcgat ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc
     3361 tgagcggtac gggcttcatt gaacaggact tcgttattgt tgaacagttg ccagtgttcc
     3421 gatagcggcg gcgcaccttc cagtacagca acattactca gaatgcgttt ttcgtcgctg
     3481 cgtggttgga tctccgcctc ttcgctttga actaatgtcc agataccatt ttgttgctga
     3541 acgctaagct gctgacgggc gctgacccct ttttcgttgc accagaaggc aatatccagc
     3601 aaatgttggt caccctgaaa acgcaaactg gcaagaccaa aaagtgaacg gtattcctca
//
LOCUS       DAJTNM010000219         3614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTNM010000219 DAJTNM010000000
VERSION     DAJTNM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.221"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..261)
                     /locus_tag="OO172_004082"
     CDS             complement(<1..261)
                     /locus_tag="OO172_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5514022.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYE"
     gene            1013..1561
                     /locus_tag="OO172_004083"
     CDS             1013..1561
                     /locus_tag="OO172_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5514023.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRNMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVEALQTKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISRATYYRLAK"
BASE COUNT         1120 a          682 c          809 g         1003 t
ORIGIN      
        1 ttcatagact cctgccgcta cgtacgggtc agcatcggcc caggcctgag ctgcttccag
       61 cgattcaaat tcagcaataa cggttgagcc agtaaatccc gcagcccctg gatcgttact
      121 gtctaccgct ggcattggac cagctgtcaa caaacgacct tcatcatgca gtaactgtaa
      181 acgtgctaaa tgtgccggac gaacggaaag gcgtttttcg aggctatcag ctttgtcttg
      241 agcgtaaata acatacaaca cgggcacaac tccttgttcg ggaaagttgt aaagtacgtt
      301 atttgaaagg gcgaagatct gcaacggaaa gatgatgtct ttgttaaggt catgcataaa
      361 gtaagggtaa ttacgccaaa aatgacattt tcactgatcc tgatcgtctt gccttattga
      421 atatgattgc tatttgcatt taaaatcgag gcctggtttt tctactgaaa tgattatgac
      481 ttcaataacc cttgaatgaa gtctattcgg ttttctagga gacctgttaa aaacaggaca
      541 atatcaggtg aatgactatg caatttaata gtagatttcc aagtatctaa gcagttttac
      601 acaaattcta tgcagtatcg gtatagtctc tttttagcat aacttattga aatatataga
      661 gaatctgtga agtctctttt cagggaacta tgcagggtgt tagtcgttcc tctctcgact
      721 cctagaaaag tgctatatac aggactatat aacgatatat agatgtttgg tcacatggtg
      781 taactatcaa gaaacttaac gatttcctac gtgaaagtta gaaagtgatg gttatagtta
      841 ccctcctaaa atcttcatag ctccttcata accagtctta aaaggacttc cttttgagac
      901 actttcaacg taacggtaga atgcaccctc aagtagtctc aaaaggttgt ttttggatta
      961 tgagacagct aaacttatga gactaacttt tgagacttct aaggggaaca ttatggcggt
     1021 gattggttat tgcagggtat caacacaaga ccagactgta gacaaccaga aactacagat
     1081 agagaaggtt cacaaggtcg ataaatggtt tattgatgag gctgtctcag gagctacaaa
     1141 ggccaccagc agagatggtt ttagaaacat gatgaactat gtaaggtgtg acgatacgtt
     1201 agttgtggta gctattgacc gtttagggcg taataccgtt gatgtgctct ctaacgtgga
     1261 ggcactacag actaaaggtg tgagggttgt ttctctacgt gaaggttttg acttattaac
     1321 accaatcggt agagcaatgc ttactatgat ggctggttta gctgaccttg aaaaagacct
     1381 tatcagtgaa cgtagaaagg ctggtattga acgtgctaaa gctgaagggg tacatatggg
     1441 gaggcctgta aaagcttctt cagaggctgt acagacgctt atttcacaag gtaagacgag
     1501 gttgcagatt caggaagaat taggaatctc tagagcaact tattacagac ttgctaaata
     1561 agttactgaa tagttacatg gatgtagctg atatttggct tttttgcaaa ggggatcaca
     1621 tagctggtgt aacaggtact tagcagctac agacatctgt cagatatttt tgaagattct
     1681 cagagaggga atttgaagag gggctactat gattttataa aaattttcag agtctgaaaa
     1741 gttactgtac agattttata caaactaaaa aggactcccg taggtgattc agtaatgaat
     1801 tacttcacga agagtccctt tacaagtcga tttttaatcg tggctacgac aatccaccca
     1861 taccagacct aaccgtaccc caccttacca agccataccc taccccgcca gactataagg
     1921 aagcgtagag aaccgatgaa ggtgtctgca cagttgcatc ttaaacaacc atcacagtga
     1981 tgtcaaatag ttcttttaag taggaggggt tcccctatgg tcacatatcc cctctctccc
     2041 gcttagtgtc ccttgatagt atctagagag tatgcctgta atgttagaga gtatgcctgt
     2101 aatgtttctg tatcaggtca gctataagag gtctggatat taccttgtaa gattcttggt
     2161 agtgattatg cagagggctt gataagagtg tttaacggga ggaagagaac catgaaaccg
     2221 aaggtaggtt ctatcttaac agtgactgta agagatacct gaaaaaatac tttaacagaa
     2281 ctaccaagaa gagagggaac ggaagagagg gaacgataag accagaggta gtgacctgtt
     2341 aaacaggctg tttcaaagtc tcttaaaagt ctgttaagag ggctaggtga acaatcctgc
     2401 cattttgtca agtgttttgt ctttagcaaa aagtgctaaa cttaacaaaa agcactaaat
     2461 gctaaagagg cgattaagaa atatactaag aggggcgctt cgcttggggt cactatgttc
     2521 tttctctccc gttaagagga tttacaggga catttcaaag ccctgataag gtaagcccga
     2581 tgataacaga aacagagggc aaaaccactt atcaacactg tgaacgaata acgtaaaggg
     2641 ctggttaaga aagtcggttt agggattggt aacaatacca attagaaggg agcttccctt
     2701 tctggggtgt cgctctatgg gtgaagttaa cctttgaagt tctctgtgaa gaaactgaac
     2761 agctattata ttggctaatt acaacccttt gaaagataag catttttaga agttactgtt
     2821 aaggttgcag agttgtctgt gaagagactt gtcagttctc tatattgggt ggttacaact
     2881 cattgaaaaa taagagcttt tagaggttac tgtcagagag gtgaatactt gatagataca
     2941 gtcttccggt tctgtcaggc tattttttaa cagatagcac aaaatgctaa agatgcccct
     3001 atgccatctc gtacaggggg gttttgaagg gagtttaacg ggggtctgtg tggtcattat
     3061 ccttaaggtc aagcgtccat gtgcgaccga acagtttaaa gacaacaagc actctgacga
     3121 ggacgtaacg aagtagcaga tctgcaataa gtccaatacc tccaccagcc catacatcag
     3181 gtgaccccag ctcaggaggg agatttacca ttagttttcc ttgtaaggcg ctctcaatcg
     3241 cattctgaca caaaaaagta cctgacctta tgagttgacc tgaagaggtc atttaactcg
     3301 ttacagagcg attgaagagg tctgtgtgca atgcaataac catgtgacca gatacatagc
     3361 cacgcctcat attttactct ccccataaac ccattacacc gcagatgtta cgaaattgtt
     3421 aaaaatactt ggtattagtc tttgaaaatg ctagcattgt atatataagt taacagaata
     3481 ataacatgtt atgaggggca gaatatgaat gagagacgca gaatttagag cgatgcttca
     3541 ggcatcaaga gaacgcaaca agcacaactc ctacgcttac acaaacaacc ccaccagcta
     3601 cgaagtccca acct
//
LOCUS       DAJTNM010000220         3558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTNM010000220 DAJTNM010000000
VERSION     DAJTNM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.222"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..346)
                     /locus_tag="OO172_004084"
     CDS             complement(<1..346)
                     /locus_tag="OO172_004084"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514024.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRD
                     YLHKVTTTVSKNH"
     gene            complement(479..784)
                     /locus_tag="OO172_004085"
                     /pseudo
     CDS             complement(479..784)
                     /locus_tag="OO172_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            852..1467
                     /gene="tnpA"
                     /locus_tag="OO172_004086"
                     /pseudo
     CDS             852..1467
                     /gene="tnpA"
                     /locus_tag="OO172_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1795..2877
                     /gene="ompC"
                     /locus_tag="OO172_004087"
     CDS             1795..2877
                     /gene="ompC"
                     /locus_tag="OO172_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5514025.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKVNL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3366..>3558)
                     /locus_tag="OO172_004088"
     CDS             complement(3366..>3558)
                     /locus_tag="OO172_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5514026.1"
                     /translation="GISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRFTE
                     RFHNRFKITPRELRKASRE"
BASE COUNT         1003 a          790 c          735 g         1030 t
ORIGIN      
        1 cgtggttttt gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg atatttgcga
       61 tacaggaatg cagtcgctgt attttgcgtt tctgcttctg ccagttgttg ctgaatttga
      121 ccttgcggct taactgtcgc tgaagtctcg ccagcgtctt ctggtttttc tgaaaactgt
      181 ttacaggctc aaagactgtg ccatctgaca gcgaggcaag tttagccacg ccagcatcca
      241 gcccgaccat tgatgctgaa gggtgaaccg gagttgatac ttcactttct gcctgaatac
      301 tgatgtacca cttaccgcag gactggctga cagtgacatt tttcacaaca cccgtgacct
      361 gacggctgtt ccggtagcgc atccagccaa gttttggcag aaaaatacgg ctgttttcct
      421 gatcgagctt aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc tttttgaatc
      481 aggggaacgc cgaccgcttc tggaaaaagt ttttgcaggc ctgctcaggg tctttcagtg
      541 actgttgcaa tggctgtgac ggagcatctt taatccattg cgtttcagtg gcatttttcc
      601 actcaaccaa tcaggaagcc attttcgcgt aagggatgtc tttgttaccg gccttatgat
      661 tctcattctg acgtgccaga gtacgactga aaacgaaacg acatgcgcct gcgaagcgcc
      721 tcatttcacg cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc tgtagtcgtt
      781 tcacagggca aattatactt tggtctgtga aaaaaactga tatccgacgc ggaaggcact
      841 gtgttttcct gatgcatgct caccgggtct ttgttaccaa gtaccgacgc cagatttttg
      901 accatgacgc gacagaaaaa ctacgtactt acttttcaaa tgtatgtgct gattttgaag
      961 ctgaactggt tgaaatggat ggcgaaccag agcacgtcca tctgttaatt aactatcccc
     1021 ccaaactggc gatatccagt ctggtaaaca aacagcctca aaggcgtatc gggtaggtta
     1081 ctgcgacggg atcggccaga tattgctgcc aggtattacc gcaaagatgt tttgtggcgt
     1141 cccggctatt ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg ccaatacatt
     1201 gaacaacagc aaacacctgg tcaggtgaaa aaacgagcct tatatccccg ccctgaagga
     1261 cgggctttta cggtgcactg gataagcctg gagattatct acacctgcat ggacatcatt
     1321 gtactatgcc ccgtacatgc cccatcacat cctcaacatc aaagtattag agccttgcaa
     1381 accattatgc gtctcagttt gtctcatcta tcttacgcaa aaaaaataga actaatatca
     1441 agcggcgcta tattgcgtat taaataaaac cacctcaatt acattaataa atctaaataa
     1501 atatcataca aataatttcc atggttattt ttaggctatt taaattaaaa aaataaatta
     1561 tgagtcaatc ataaataaca acctttaata taatttgact accaaattta tattcgttca
     1621 caaaatagtc atgcattgtg tgcaatagaa acagttcaga taaaaatagg gatctacttc
     1681 acaatttaaa tgagaaacta aaacttacat cttgaaataa tctcattgat tggatgaata
     1741 tttgtagtgc agtgacatca tttttaaata atagttcaaa taaaagggct cacgatgaaa
     1801 aaattaacag tggcaatttc tgctgtagct gcatcagtac tgatggcgat gtctgctcag
     1861 gcagcagaga tttacaataa agacagtaac aagctggatc tgtacggaaa agttaatgct
     1921 aagcactact tctcttctaa cgatgcagat gatggcgaca caacttatgt tcgtctgggc
     1981 ttcaaaggcg aaactcaaat caacgatcaa ctgactggtt tcggtcagtg ggaatatgaa
     2041 ttcaaaggca accgtgctga atctcaaggt tcttccaaag ataaaacccg tcttgccttc
     2101 gctggcctga aattcggtga ctacggctcc atcgattatg gccgtaacta cggtgtagca
     2161 tacgatatcg gtgcatggac cgacgttctg ccagaattcg gtggcgatac ctggacccaa
     2221 acagatgtgt tcatgactgg tcgcaccaca ggtgttgcaa cctatcgtaa caacgacttc
     2281 tttggtctgg ttgatggtct gaactttgct gctcagtatc agggtaaaaa tgaccgcact
     2341 gacgtaactg aagctaacgg tgatggtttc ggtttctcca ctacttatga gtatgaagga
     2401 ttcggtgtag gtgcaaccta tgctaaatct gaccgcacta atgatcaggt tatctacggt
     2461 aacaacagcc tgaatgcatc tggtcaaaat gctgaagtat gggcagctgg tctgaaatat
     2521 gatgcgaaca acatctatct ggctaccacc tattctgaaa cccagaacat gactgttttt
     2581 ggtaataacc atattgccaa caaagcacaa aacttcgaag tagttgcaca atatcagttc
     2641 gacttcggtc tgcgtccgtc cgttgcttac ctgcaatcta aaggaaaaga cttgggtgcg
     2701 tggggtgatc aggacctgat tgaatatatt gatgtaggtg caacctatta cttcaacaaa
     2761 aatatgtcca cttttgttga ttacaaagtc aacctgattg ataagagcga tttcacgaaa
     2821 gcatctggcg ttgctaccga tgatatcgtt gctgtaggat tggtttacca gttctaatct
     2881 gattactcaa agatatgttg tgggaggctt tgcctcccca acatataagt ggcgccctca
     2941 agccactttc cttagaagca ctatcttgct tctgactata taaattaccc tttattttct
     3001 gggacgtttc aacgccccat ttttaataat actcagtgaa caattaacgt atcaattaga
     3061 tttaataaca acgatatccg tacctaacct gatatctaat gtcataagca tccctttaat
     3121 tatcccctca acctcttgta aatttcccac tcttgagctc cacatagtcc aaacgccgga
     3181 tgcaaaaaaa atcacacgtg catctctgaa attgtgatgg aggaagtgag tatcaattat
     3241 atgtgcttat aaacgttccc gattggcaca gaagtcaccg gattgttcag gctccaatag
     3301 gttgagtatg tcgtatttat tttccaaaat aaaaatcgat gctgttgctc cttaataaaa
     3361 tacttttact ctctggacgc ttttcttagc tctcttggtg ttatcttaaa acgattatgg
     3421 aacctttcag taaaacgaga aggacacttg taaccatttt ccctggcaat ctcgctgatg
     3481 ggttttactg tcgtttgtat agcagacaac gcattattta acctcacgtc gtccaatata
     3541 ctttgaaaac ttattccc
//
LOCUS       DAJTNM010000221         3541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTNM010000221 DAJTNM010000000
VERSION     DAJTNM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.223"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..646)
                     /gene="sitD"
                     /locus_tag="OO172_004089"
     CDS             complement(<1..646)
                     /gene="sitD"
                     /locus_tag="OO172_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5514027.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLT"
     gene            complement(643..1500)
                     /gene="sitC"
                     /locus_tag="OO172_004090"
     CDS             complement(643..1500)
                     /gene="sitC"
                     /locus_tag="OO172_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5514028.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1497..2324)
                     /gene="sitB"
                     /locus_tag="OO172_004091"
     CDS             complement(1497..2324)
                     /gene="sitB"
                     /locus_tag="OO172_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5514029.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2324..3238)
                     /gene="sitA"
                     /locus_tag="OO172_004092"
     CDS             complement(2324..3238)
                     /gene="sitA"
                     /locus_tag="OO172_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5514030.1"
                     /translation="MHSIKKVTMLLGCLALTCSITFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT          974 a          889 c          847 g          831 t
ORIGIN      
        1 gtgtcaggag tactgcaatg gcgccaggcg caatgagcag agaaatagag agaatgatac
       61 ctactgcttt cagcgtcgcc acaatggtca gggaaaccat gcacagcagc ccataatgca
      121 gccatctcgt atgcaaaccg cttacctgcg cctgttgata atcaaagctg aacagcaaaa
      181 aatcccgcca ttttacgcta ataacaagtg taaccagccc ggcaataatc accgtctgga
      241 ttatatcgcc gatggttatc ccgagcatgt cgccgaaaag aatatggtca agatgtacgt
      301 ctggtttaat tgcaatatac aagatcagac ctgcggcaaa catgccagaa aaaacaatcc
      361 ccataactgt atcttgcttg attcggctgt tgtctttcag gtatcccgtc gccaccgcgc
      421 agaatactcc ggcgacgaat gcccctgtag ccagaggtaa ccccagaatc caggtaagca
      481 cgatgccagg aaaaaccgca tgactcatcg cgtcacccat cagtgcccaa cctttcagga
      541 ccagaaagac cgacagcagc gcacagggta tggaaactac cagtgaaatc agcagtgcgt
      601 gactcataaa ggtgaactgc agtggctcca gtaataatgc catcatgagc gatcctccag
      661 cgctttatgc gcacgccgac gatttgccaa aagtccatgg gtgggcgcaa atacaaatgc
      721 taaaagaaac agcagtgtct gcgccaccac gatgattcca cctgtcgcgc cgtccagaaa
      781 gtaactgacc cacgcgccga ggaaactggt tacactgcca atagttacag caattatgag
      841 caggcgggga aaacgatcgg ttaacaacca tgctgttgca ccgggagtca ccaccagaca
      901 gatcaccaga aaggcaccaa ccgtttgcag cgccgcaacg gtcgaaaccg acagcagggt
      961 gaagaagagg atttttaatc tgcccggatg taatccaata gcacgggcgt gattctcgtc
     1021 gaaaaaggtc accataagat ctttccattt gaaaaaaagc acgatgatcg agagaatgcc
     1081 aatgatagtg agttgcagga tatccgccgg atcgattgcc agaatattgc ccaggacgat
     1141 ggtctgaatg ttcacggatg tcgggttcaa tgacaccata aacagcccca ggccaaaaaa
     1201 ggacgagaag atcaggccga tgatggcatc ttcttttaag cgggtgcgtt ggttaagaaa
     1261 cagcatgctg cccgctgcca gtccgccgga aaagaaggct cccagagaaa acggaagccc
     1321 cagcatatat gcccccgcaa cacccgggac aatagagtgc gacagcgcat cgccaatcag
     1381 cgaccagcct tttagcatca gatagcatga caggaaagcg cagagaccgc cgaccattgc
     1441 cgagacccac atcgcattga gcatatattc atagctgaag ggttccagta gcacgttcat
     1501 cttgcttccc tctgcaccgc tgacggtcga tgtgccacaa aagggcgttc atcgtcagta
     1561 ataatgcttt cttccgagcc gttaagtgtg acatggcgca gtacgccgct aaaagccagc
     1621 tccaggttct cggctgtaaa agtggtgtct gtcggcccgc ttgccaggac ggtgcctttg
     1681 accatgaccg tataatcgca aaatgtcgtg actgagccga gattgtgcgt tgagacaagc
     1741 atcgttttgc cttccgcgcg taactccctc aacaggctga tgattttcgc ttcggttttc
     1801 acatcaacgc cggtaaaagg ttcatccagc aggatcacat caccctgctg tgcaatcgct
     1861 ctcgccagaa agacgcgttt cttttgtcca ccggaaagct cgccgatttg acgatggcga
     1921 aaatccacca tatcgacacg ttccagcgca tcggtgacaa tctgacgatc cctctttttg
     1981 gctatacgca acatacccat atgcccatag cggcccatca tcaccacatc ttccaccagt
     2041 acaggaaatg accagtcaac ctcttctgac tgagggacgt aggcaaccag gtttttctgt
     2101 agcgcctgtc gcgtgggaat acccagaaca gatatctttc cgctggtcag acgcacaaat
     2161 cccataatcg ctttaaacaa cgtcgatttt ccggaaccgt ttaccccaac cagagcggca
     2221 atagatccac ttggtactgt gaaggatgca tcgcgcagtg ctgtgtgccc gttacgccag
     2281 gtgacagtaa catcattaac gacaatgcct gcagattgca tcattatttc tcccttttcc
     2341 cggctttaat tccctgtacc agcgtactgg tagtaacctt aagaaggtcg atatacgtag
     2401 gtaccgggcc gttttctgtg ctcagggaat cgacatagag cacaccgccg tagtgtgcgc
     2461 cggtttcacg cgcaacctga cgcgctggtt tatcggaaat cgtactctcg ctaaagactg
     2521 ccgggatatg atttttctta actatatcaa caaccttacg tacctgctgc ggtgttcctt
     2581 gttgatcggc attaatcggc cacagataaa gctcttttag ccccaaatcg cgtgcgagat
     2641 aagaaaaagc cccttcactg gtgaccatcc atcgctgatt ctcagggagt tccgtaatct
     2701 gcttacgcag gggggcaagg gtttgggtaa tcttggcttt ataagtatcg gcattacgtt
     2761 ggtaggtttg tgcatttgcc ggatcgtatt ttatcagcgc atcacgaata ttatcgacgt
     2821 aaatcagagc attatctggc gacatccatg catggggatt aggtttgccc tcatagggtc
     2881 cttcggtgat ccctactggc gtcacacccg aagagacaat tacttctgga accccattga
     2941 gatgctggta aaagcgttgg aaccacaatt ccagattcat accattggcg agaatcagtt
     3001 gtgccccctg cgcacgttta atatcgccag gggtaggctg atactcatga atttctgcac
     3061 caggcttggt tatggatgag acttctgcag catctccagc cacgtttttg gccatatccg
     3121 cgatgatggt aaatgttgta atgaccttga atttttcagt tgcacttgcc tgaaatgtga
     3181 tcgagcaggt gagggcgagg caccccaaga gcatggttac tttttttatt gagtgcataa
     3241 tttagtacct atagtgagca aaattagaac aaacatagca acggctatgt tttatagcat
     3301 aacctataca aaatgaaaat aattatcatt aacagctttg aggcctaaaa cagggattag
     3361 gttgtttcct gctggcgaca tgatattgag gaaaaaagta gagcagctat taggctttga
     3421 aatgacttta tgaggaaaat tgttgatggc aaaaatgggg caaaacgctg tataaggggc
     3481 aaaaaagggg caaaaaagag tgggttatcg tagcttattg ttgtcgctga taatgtttaa
     3541 c
//
LOCUS       DAJTNM010000222         3370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTNM010000222 DAJTNM010000000
VERSION     DAJTNM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.224"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..924)
                     /locus_tag="OO172_004093"
     CDS             complement(<1..924)
                     /locus_tag="OO172_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HCR5514031.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFIVYSFACFFIGKFIAN"
     gene            complement(952..1872)
                     /gene="rbsK"
                     /locus_tag="OO172_004094"
     CDS             complement(952..1872)
                     /gene="rbsK"
                     /locus_tag="OO172_004094"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5514032.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGMQSSYPSIEQFNEFLTLNE"
     gene            2178..2960
                     /locus_tag="OO172_004095"
     CDS             2178..2960
                     /locus_tag="OO172_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5514033.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPALSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(2962..3188)
                     /locus_tag="OO172_004096"
                     /pseudo
     CDS             complement(2962..3188)
                     /locus_tag="OO172_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          998 a          771 c          669 g          932 t
ORIGIN      
        1 gttggctata aacttgccaa taaagaagca ggcaaagctg tagactatga agtttgaagc
       61 atcacgttcg ttgatatcgc ccaactccag tgccagacgg atggtaaatg accatactgc
      121 gacctgcata cccacataaa ggaattgagc cacaatacca cgacgaaagc gcgaattttt
      181 agccagatag cgcagcgtat ccattgctga cgggcgttta tggtgacttg tctgtgccac
      241 tttacaggtt gggaagcggg ttaaaaggaa caacaccatg accacaacca gaatcataat
      301 catatactta tacggttcaa gggtgttctc taacatcagc accttaaagt tgtgaatttg
      361 ctcggcgttc attccggaca tctgcttctc aaggctttcc ccctcggaga aaaccagata
      421 tttgcccaat aaaataccag acgcagcacc aatcggataa aaggtctggc tgatattgag
      481 ccgcaatgtg gcataggctt ttggaccgat cattgaactg tatgtgttcg ctgcagtttc
      541 aaggaaactc aggccaatcg caatcgcaaa aatagctgca aggaacatag tgtaggttgc
      601 catatgcgag gcagggaaaa aaagtgtaca accaccaata tacagcgtca ggccaattaa
      661 aattgccacc ttataactgg tctttttaat cacaagggat gctggtattg caattaaaaa
      721 ataacctcca taaaatgcgc tctgcaccaa tgctgaagca aagttactta gcgaaaatac
      781 acttttgaat tgagtgatta atatgtcatt taatgcagct gcgcatcccc atagcgggaa
      841 taaacacgat aacaaaataa actggaacaa gggagtctta ttcagatacc catcaggcat
      901 ctgaatgatg tttttatcgt tcatagtgct acctttaact gtgcaggatg attattcgtt
      961 taaggttaaa aattcattaa attgttcaat actcggataa gatgattgca tacctttccc
     1021 tgtgacgctg aaagcggcaa agagagcggc ttttttcaaa gcggcttcaa catcaccgct
     1081 ttgaacataa taatgggaaa agcaaccaat aaatgcgtca ccagcgccac tagtatcaac
     1141 agcatttact ttgaatgcag gaacatggac ttcctgatcg cgggtcatcc ataatgcgcc
     1201 tttttcgctc atggtaacaa taatattgtt cagcccttta tcaactaacg aacgtgcggc
     1261 caaacgaata tgatcataag tatcaaccga cataccagtt aatatttcca gttctgtttc
     1321 attcgggata aagaaatcac ctttgcaggc ataagacata tctaactcac gcaatgccgg
     1381 agccggattt aataacactt caataccatt tttcttacca aactcaatcg cgtggtaaac
     1441 tgtttccagt tgaacttcca gttgtaaaac gatcaatttg cattttttca gatcttctgc
     1501 agctcgatcg atatcttccg gggaaagaaa tttattcgct cccttaatta ttaatatact
     1561 attgctcgag ttggcattaa caaagatcgg tgcaacacca ctgctggtac aggggacctt
     1621 ctcaacataa gtggtattaa ttccccatga ttcaagatta cgaatagtat tatccgcaaa
     1681 aatatcatca cctactttag tcagcatcag gacttttgaa ttcaatttag ccgccgccac
     1741 cgcttgatta gcacctttcc caccacatcc gattttgaag gcaggtgctt ccagagtttc
     1801 tccttcttta ggcatctgat tagtgtaagt aatgagatcc accatattgg aaccaataac
     1861 tgcaatgtcc atttcactac ctcttataaa ctttcgcata acaatggtat ttaaataaca
     1921 ttagcatgtt acttttgcat catttgtgac tgagatcgcg attagcacat caacccgatg
     1981 tttatttaat agacttccag tctcatcact caggccaaca ctatctaatc ataagcaacc
     2041 taacaagatt agtgcccaaa attcagcagc ctataccctt ttcatttcaa aggggcggtc
     2101 gtatagtatg gtaatgaaaa caatgtttac taacgccaaa atgttatttt tataacattc
     2161 ttacggagag agagttgatg gaaacgaagc aaaaagagcg tatccgacgt ttgatggaac
     2221 tgcttaagaa aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg
     2281 taatgactat tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg
     2341 gtggctatat tgtaatggtg aataaacccg cgctatccat gccagtaatc catgacgttc
     2401 caaaaaatca tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa
     2461 atgatctgat cttctttgat aatggccagg agataccact cgttataagc atgatcccgg
     2521 atgcaatcac cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa
     2581 agcctaatgt aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt
     2641 acgatgccag taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg
     2701 ccagcggtgt gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta
     2761 agcgtaaagc gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg
     2821 atgaagtggc ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc
     2881 gtccgttacc ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac
     2941 ctgattcaga agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg
     3001 gttggactga ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac
     3061 ctactccaga cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg
     3121 aatcggcagg cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg
     3181 tgagtcatta cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt
     3241 ccataatgtc tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca
     3301 ctggcgctgc cagccggttt gaattcatct cacaaccctg catagagcga atctcttgcc
     3361 tgcacgtcac
//
LOCUS       DAJTNM010000223         3230 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTNM010000223 DAJTNM010000000
VERSION     DAJTNM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.225"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            99..2096
                     /gene="betT"
                     /locus_tag="OO172_004097"
     CDS             99..2096
                     /gene="betT"
                     /locus_tag="OO172_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR5514034.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2159..>3230
                     /locus_tag="OO172_004098"
     CDS             2159..>3230
                     /locus_tag="OO172_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5514035.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGAS"
BASE COUNT          749 a          751 c          822 g          908 t
ORIGIN      
        1 gcattacgat taacgccgca tagccagttg aactttgcta ctttgtgaga ggtagtacct
       61 tctatccagt gcgaatttaa ttaatggaat aaatgattat gagtgaaaat gatacaatcc
      121 caaagaagtc tacaagtcag attaacaaag cggtattctt tacatctgct ttgctaattt
      181 tccttcttgt cgcctttgcc gccgtattcc cggatgtcgc cgacaaaaat tttaaactac
      241 ttcagcaaca aatcttcacg aatgccagct ggttctacat ccttgctgtg gccctgattt
      301 tactgagtgt cacgttcctt ggactctcac gctacggtga tatcaagctg ggcccggacc
      361 atgcgcagcc tgatttcagc taccactcct ggtttgcgat gcttttttcg gcagggatgg
      421 ggatcggcct gatgttcttt ggcgttgccg aacctgtaat gcattatctt tcgccacccg
      481 ttggcactcc agaaaccgtt gcggcagcta aggaagcaat gcgtctgacc tttttccact
      541 ggggactgca cgcatgggca atttatgcca ttgtggcgct gattctggcg ttcttcagtt
      601 accgtcacgg tctgccttta actctgcgct ccgcactcta tcccattatt ggcgatcgca
      661 tatacggacc tgtaggacat gcggttgata ttttcgctgt tataggcacg gtctttggcg
      721 ttgcgacatc actgggttac ggtgttttgc aggtgaatgc cggtttgaac catcttttcg
      781 gggtgcccat caatgaaacg gtgcaggtaa ttctgatcgt ggtcatcacg gggttagcga
      841 cgatttcagt ggtgtccggt ctggataagg gaatacgtat cctgtctgaa ctcaatctgg
      901 gtctggcttt gttgctcctg gcgctggtcc tgtgtctggg accaaccgtg cttctgctga
      961 agtcatttgt ggaaaatacg ggcggttatc tttcggaact ggtgagtaaa acgttcaacc
     1021 tttacgcgta tgagcccaag tcgagcaact ggctgggggg ctggacatta ctgtactggg
     1081 gatggtggct ttcatggtcg ccgtttgtgg ggatgttcat cgcacgggtc tcccgcgggc
     1141 gaaccattcg cgagtttgtc accggcgtgc tgtttgttcc cgcgggtttt acgctaatgt
     1201 ggatgacggt gtttggtaac agcgcgatct atctcattat gaaccagggg gccacagacc
     1261 tcgccaatac tgttcagcag gatgtggcgc tggccctgtt taatttcctg gagcatttcc
     1321 cgttctcttc tgtgctgtca ttcattgcaa tggcgatggt catcgtcttc tttgtaacgt
     1381 ctgctgattc gggggcaatg gttgtggata ctctggcatc aggtggagtg gcaaacacac
     1441 ccgtctggca gcgaatattc tgggcctcgc tcatgggcat tgttgcaatt gcgcttctcc
     1501 ttgccggagg gctaagtgcg ctgcaaacgg tgacaatagc gagtgcattg cccttctcag
     1561 tgatcttact aatatctata tacggacttt taaaagcttt gcgccgggat ttgaccaagc
     1621 gtgaaagcct gagcatggcg acaattgctc ctacggctgc acgtaaccca attccttggc
     1681 agagaaggtt acgcaatatc gcgtatctgc cgaagcgatc tcttgtgaaa cgttttatgg
     1741 acgacgttat ccagcctgcc atgacgctgg ttcaggagga actgaacaag caggggacga
     1801 taagccacat tagtgatgca gtcgacgatc gtattcgtct tgaagtcgat ttgggcaacg
     1861 agctgaattt tatatatgaa gtgaggcttc gcgggtatat ctcaccgacc ttcgcgctcg
     1921 ccgcaatgga taatgatgag cagcagactg aacaacatcg atattatcgc gctgaggttt
     1981 atctcaaaga aggcggtcaa aattatgatg tgatgggctg gaaccaggaa cagctgatta
     2041 atgacatact ggaccagtac gaaaaacacc tgcacttcct gcacctggtt cgttaatagc
     2101 aacatgccgt cctgggggcg gcaattatta tcccggccgc aatatgaggg aatgcagaat
     2161 gatttcacgc tggaaatgga tgctgaagca gacaattaaa aaactatggt tcagggcaac
     2221 gttattcgca attgtcgcga taataacggc ccttttatca attcttttta aatcaatgat
     2281 acctgagtcg gtttccgtga aggttggtgc ggaagcagtc gataacattc tgaacatact
     2341 ggcatcgagt atgctggcag tgaccacatt ttcgctgagt atcatggtca cagcctacgg
     2401 ttcagccact actaatgtga ctcccagagc tacgcgttta gttgttgaag acgtcaccac
     2461 acaaaatgta ctggccacct tcatcggttc ttttctcttc agtctggtag ggattattgc
     2521 cctcaatatg ggagcttatg gagaaagggg gagagtcatt ttattcattg tcacactggt
     2581 tgtcattgcc ttaatcctca tcacattgct tcgctggata cagcatttga cctctctggg
     2641 gagggttggt gagacaacgg cacaagtaga acaggcggcc atcgaaacat ttattgcgag
     2701 agcaagaaat ccctgtctcg gtggatatcc atggcttgag aacaatgaac agccgaaagg
     2761 aacggttgca gtttatccga agaagattgg ctatgttgaa tatattgata tggtgaaact
     2821 tagcaagctg ctgaccaatg atccccgtca tgtatacctc gtggcgcagc caggcagttt
     2881 catacatccg tccatgccag ttttgtacct gagtcagggc caggagtcgt caatcaacac
     2941 cgatttactt gagacgatta ttgtctcgga tgcacgttca tttgctcagg atcctcgatt
     3001 ttgtcttagc gtcatggccg aaatagcctg ccgggccctt tcccctgcag tgaacgatcc
     3061 tggaaccgcc attgatgtca ttggcagagg tgttcgtata ctttccactt acgcgcagaa
     3121 taaatctgat gaaatagaag tgaaatatcc ttcagtacat gttgcaccac ttcagaataa
     3181 cgatctactg gaagactttt tctcacctgt cgcgcgcgat ggtgccagta
//
LOCUS       DAJTNM010000224         3218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTNM010000224 DAJTNM010000000
VERSION     DAJTNM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.226"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..1216)
                     /gene="cusS"
                     /locus_tag="OO172_004099"
     CDS             complement(<1..1216)
                     /gene="cusS"
                     /locus_tag="OO172_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5514036.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVD"
     gene            complement(1206..1889)
                     /gene="cusR"
                     /locus_tag="OO172_004100"
     CDS             complement(1206..1889)
                     /gene="cusR"
                     /locus_tag="OO172_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5514037.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2046..>3218
                     /gene="cusC"
                     /locus_tag="OO172_004101"
     CDS             2046..>3218
                     /gene="cusC"
                     /locus_tag="OO172_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5514038.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKMEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQI"
BASE COUNT          791 a          832 c          846 g          749 t
ORIGIN      
        1 gatcgaccgt ctgacagcgc actacaattg tctctctggt tggcgtataa cgcagggcat
       61 tagaaagcag gttgcttagc gcccgacgca gcatcaacgg atcgcccgca acctgacgtt
      121 tgtcgccaac aaaccgcaac tccacgccgc gatcttccgc tagcgcctcg aaaaaatcga
      181 acactttgcc gacttcatcc gccaggttga gcattttctt ttcggggatt agctggttgt
      241 tatcggcctg agcgagaaac agcatatcgc tgaccatttt cgccattcgc gtcagctctt
      301 cgagattaga gtagagcaca tcttccagct ccttctggct gcgcgactgg ctgagggcga
      361 tttccgtttg cgtaatgaga ttagtaattg gtgtgcgaat ttcgtgggcg atatccgctg
      421 agaaattgga ctggcgagta aagacatcct caatacgctc gatcatatgg ttgaacgaca
      481 gtaccagctg ttccagctca atcggcacgg tctgcgggtc aaggcgaacg tcgagatctt
      541 tcgaggtgat attctggatt tgacggctga cgctgcggat cggcgcgtga cctttatgta
      601 ccgccaacag cacgataaag acgatcagga tgctgattac cgatgcggtc ataataagtt
      661 tattcatcaa atcatttatg taatgaagat gaaaatcgat cgacagcgcg atgtagagcg
      721 tataaatcgg tttgccgtcc accaacgggc caaccggcaa gttaatcatc cgccagttgc
      781 tgtgttccat atgcccgtga ccgtggcctg gcatcataat cgtcgggccg gaaaggagat
      841 acacctcgcc accccgcgcg tctttatcgg gtatggcatc acgtgcaaac tcgcggatat
      901 caggcgcacc gggggagtga tacaccgttt tgccgtggct atctgccagg gaaatcaaca
      961 cgttggaata accactgacg atatcttcca gcgtcattaa gcgtcgggct tgcgtttcgt
     1021 cagggtgatt tagtacccgt tcaagggtgg cgctaatctc ttttaaatca ttaatgtcct
     1081 gctcggcaaa atgcactttt actgagtgga tcatgatcca tgcaaaggcg aaaaacgccg
     1141 cgatggtggc caggctgata aaaaaggtca ggcgggttgc cagcgaaaac gggcgctgaa
     1201 atggcttact gaccatccgg cacctcaagc atgtaaccca cgccgcgcac ggtttgaatc
     1261 agcttcggct caaagtcgtt gtcgattttg ccgcgtagcc gcttcaccgc cacatcaatg
     1321 gcgttagtgt cgctgtcgaa attcatgtcc catacctgcg aggcgataag cgagcggggc
     1381 agcacttcgc cctgatggcg gagaaaaaac tccagcagag taaattcttt actggtcaga
     1441 gtgatgcgcg tgccgctgcg ggtgactttg cggctgacga gatcgaccat cagatcggca
     1501 acctgaaact gactttcgat aatcaccgcc gccccgcgcc gcaggagagt gcgcacccgc
     1561 gccagcagtt cagcaaaagc gaacggtttc accaggtagt catctgcccc caactccagc
     1621 cccttgacgc gatgttcaat ggtgccaagc gcggtaagca acagaatcgg catcccttta
     1681 ttggcggagc gcaacatgcg cacgatatcc cagccgttca cgtctggcag cataatatcg
     1741 aggattatca ggtcataatc accggtcatc gccagatggt agccattcag cccgttgtcg
     1801 gccaaatcga ccacaaaacc ggcttcggtt aaccctttgg tcaagtattc tccggttttc
     1861 ttttcatctt cgacaatcaa cagtttcata tttcctccgc atgttgcccg ggcaattcta
     1921 gagtagcggg atcagatggc aatcgcttat tggcaaaatg acaattttgt cattttcctg
     1981 tcaccggaaa atcagagcct ggcgagtaaa gttggcgaca taaaatcacc agaaattatg
     2041 agcctatgtc tccttgtaaa cttctgccat tttgtgtggc ccttgcgcta actggttgtt
     2101 cactggcacc ggattatcag cgtccggtaa tgcccgtacc gcagcagttc tcactcagcc
     2161 agaacggcct ggttaacgca gcggataact accagaacgc gggctggcgc accttttttg
     2221 ttgataatca ggtgaagacg ctgattagcg aggcgctggt gaataaccgg gatttgcgca
     2281 tggcgacgct gaaagtgcag gaggcacggg cgcaatatcg tctgaccgat gccgaccgct
     2341 acccacagct caatggcgag ggcagcggca gctggagcgg caatcttaaa ggcgattcag
     2401 ccacgacgcg ggagttctcg actggcctta acgccagctt tgatctcgat tttttcggtc
     2461 gcttaaagaa catgagcgaa gccgagcgcc aaaattattt agccactgag gaagcccagc
     2521 gcgcggtgca tattttgctg gtttctaatg tcgcgcaaag ctatttcaat cagcagctgg
     2581 cgtatgcgca attgcaaata gccgaagaaa cgctgcgtaa ttatcagcag tcatatgcgt
     2641 ttgtcgaaaa acaactgttg accggtagca gcaatgttct ggcgctggaa caggctcgcg
     2701 gggtgataga aagtacccgc agtgacatcg ctaaacgtca gggggaactg gcgcaggcga
     2761 ataatgcatt gcaactgtta ttgggaagct acggcaagct accgcaagcg cagacagtaa
     2821 acagcggcag cctgcaaagc gttaaattac cggcgggctt gtcgtcgcaa atcttattgc
     2881 agcgcccgga tattatggaa gctgaacacg cgttaatggc ggctaatgct aatattggtg
     2941 ctgcgcgtgc ggcatttttc ccgtctatca gcctgaccag cggaatatca accgccagca
     3001 gcgatctgtc gtcattattt aacgctagca gcgggatgtg gaattttatt cctaaaatgg
     3061 agatccccat ttttaatgcc ggacgcaacc aggccaatct ggatatcgcc gaaattcgcc
     3121 agcagcagtc ggtggtgaat tatgaacaga aaatccagaa cgcttttaaa gaggtggcag
     3181 atgcgctggc attacgtcaa agcctggacg atcaaatc
//
LOCUS       DAJTNM010000225         3176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTNM010000225 DAJTNM010000000
VERSION     DAJTNM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.227"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            846..1040
                     /locus_tag="OO172_004102"
     CDS             846..1040
                     /locus_tag="OO172_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5514039.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESEIGT"
     gene            complement(2065..3174)
                     /gene="fepE"
                     /locus_tag="OO172_004103"
     CDS             complement(2065..3174)
                     /gene="fepE"
                     /locus_tag="OO172_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5514040.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          941 a          781 c          657 g          797 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagata tttaacataa tatcttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc ccctaatata agatgatgtt tttttatgaa
      421 aatagtcagc ccccccccct aaaaagcggt gttggcgcag ctacaacacc gcgccgacac
      481 cgctttttta aatatcacaa agagagcaag aagaactaat ttgtcataac tctctattta
      541 taaagaaaaa tcagcaaaaa cttgtttttg cgtggggtgt gggcatttca ggcatgagaa
      601 cagactcgcg tttttccgga gtatcagcgg aattattgat taaggaacac tcgacgcaga
      661 gcgaaatttt atgcaacagc ggctgcactg cacagggatt atttatcagt tgatatgctg
      721 tgccatgata cgtcctaaag aaacaggaca ggatgtgcaa ttgtaatacc gtcgcccgca
      781 acgtcttaca attaccacct gctcagcccc ccccgacacc cggaactgac ggagaacaac
      841 ggaaggtgaa caaacaacaa caaactgcgc tgaatatggc gcgatgtatc aaaagccaga
      901 gcctgacact gcttgaaaaa ctggatgctc tggatgccga cgaacaggcg gccatgtgca
      961 aacgactgca cgaactcgca gaagaactcc agaacagcat ccagattcgc tttgaggccg
     1021 aaagtgaaat aggaacataa cgaagctgac ggaggcggtc acggctttcc cgtgggcgtt
     1081 ttcgtcagca gacaagcccg tcagggcgcg tcagggggtt ttagcaggtg tcggggcgca
     1141 gccatgaccc agtcacgtag cgatagcgga gtatatactg gcttaatcat gcggcggcag
     1201 agcaaatggt gagagaacag cgacatatga ggtgtgaaat gctgcacaga tacgtaagga
     1261 gaaaatgcag cttccggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1321 ttcggctgcg gcgagcggtg tctgctcacg caaagacgga tatacggtta tccacagaat
     1381 caggggataa caccagaaaa aacatgtgag caaaaaagag gccccgaaaa aaggccgcgc
     1441 cggtggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaac cgacgctcaa
     1501 gtcagaggtg gcgaaacccg acaggactta aagataccag gcgtttcccc ctggtggctc
     1561 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
     1621 gcgttcgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
     1681 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1741 ttgagtccaa cccggtaaga cacgacttaa cgccattggc agcggccact ggtaaacagg
     1801 atgtgcgaga gatgtattga gagttcttga agtggcagcc ttactgaggc tacactagaa
     1861 ggacagattt ggtgacatgt ctcgcacaag actgttacca cggttcagaa gttccccaac
     1921 ttactgaacc ttcgaaaaac cacctcccca ggtggttttt tcgttttcta gagtagaaga
     1981 ctacggctgt aagggaatga tctcaagaag atcctttgaa ctttttataa aagttgctta
     2041 taaaatgatg catcagcaca acttttactc aatcacactc atctttctgg cctccatggc
     2101 atgacgtaaa agtatatagc cacatgcaag catccctcca atgacagaag aaagaattac
     2161 aattatggca ttacttggcc catcctttct gaccggtaac gttgggctca attgatactg
     2221 aaatggcata aaatcaacat cccccacttt cgccacaacc aattgattca caacatattg
     2281 acgatttcgc aactccccac tcaattccga aacatccttg attgattttt tgatgttcaa
     2341 tttagttgct ataccatcag aaccgagagc cacaggaaaa tctgggtcat ccttcataat
     2401 ctgaccattg ctgtatacag gtttttttat tccagccgca ttagcaacct ccagtgaata
     2461 attgaggcgt cgaatatctg cattcagctg gtttctaatt tttatctcat ccagtgcaag
     2521 aatttctttt tcaacattag ttttaacttc tagtttattt cttatatctt ccatcaaatc
     2581 ccttacaaca agggaagaaa cataattaat atacccagac aacacatcgt gagcttcttt
     2641 acttgttgga gcagtaaaac tcagagtcca tgatacatac aatgatgact tgtcgttttt
     2701 cttactggca ttatcatcaa tcgacttcat atttttactc aactcaacaa ttgcacgatg
     2761 cagttcgaga gcgctaatgt tattcttttt aataatctcc ataacataag gagatgatct
     2821 catatactca ttcaacgaat taacggactg aaatttctta ataaacagag caaatgcgct
     2881 atcacgattg atatcaaaat ccactcccag aacatgaagc ttggaaagct ccttatttaa
     2941 ttcactccat tgaacagact cagcaggagt aaccacagca gaactagtcc atttttgtgg
     3001 caaaacaaaa gaaacaagaa agccaataca agcaaaaaat agaatcaccc caacaatttt
     3061 tatttttgct gcccataaaa cgctaatcaa gccaaaaagg tcaatttctt cattaccatt
     3121 cggcaacatg ttgtaattag aaaaatgttc gtcagagacc tttgagattt tcattg
//
LOCUS       DAJTNM010000226         3151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTNM010000226 DAJTNM010000000
VERSION     DAJTNM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.228"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..475)
                     /gene="cybB"
                     /locus_tag="OO172_004104"
     CDS             complement(<1..475)
                     /gene="cybB"
                     /locus_tag="OO172_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5514041.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAH"
     gene            664..1665
                     /gene="gap"
                     /locus_tag="OO172_004105"
     CDS             664..1665
                     /gene="gap"
                     /locus_tag="OO172_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5514042.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDNTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVMQLIRTLEKFAKL"
     gene            complement(1707..3146)
                     /gene="aldA"
                     /locus_tag="OO172_004106"
     CDS             complement(1707..3146)
                     /gene="aldA"
                     /locus_tag="OO172_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5514043.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
BASE COUNT          825 a          829 c          715 g          782 t
ORIGIN      
        1 ggtgtgccag tgcagccgca gcgtgcaacc cgatgacaaa atatcccaga ttcgccagcg
       61 tctcatgcca cgactttaag ctatcaaccc gttcgaaatt ggcctctgaa gcgtaaggca
      121 tcgtcaaacc aaacgcaaac cacgggttgc cccggttata catcatcacc aaaccaatca
      181 ctggcagcgc aataaacagg agataaatca ccaaatgtcc caaatgcgcc agtcccgtca
      241 tcatcggttt cggcttaggt ataatcggcg gggttgggta tttcagtctt aacaaaagac
      301 gaaccaccat cagcacgaga attgagatgc cacaggaaac atgaatcatg ttgatgagcg
      361 gccgatcgct acgtgggaag aaaccacgaa actccattgc gcaatacgct gcgataacca
      421 gcaaaaagac cagccagtga atgctgattt gtaaccttga gtatttattt tccataacat
      481 ttcctgcttt aacataattt tccgttaaca taacgggctt ttctcaaaat ttcattaaat
      541 attgttcacc cgttttcagt acgttacgtt tcactgtaag aatggagatt gcccatccca
      601 caatcctggt ctaagcctgg aaaggatcaa ttttcatccg aacgttcctg acaggagaaa
      661 accatgagta aagttggtat taacggtttt ggtcgtatcg gtcgactggt gttgcgtcgt
      721 ttacttgaag tcaaaagcaa catagacgtt gtcgctatta atgatcttac ttccccaaaa
      781 attctcgcct atctgctgaa acatgattca aactatggtc cgttcccctg gagcgttgat
      841 tttacggaag attcacttat cgttgatggg aaaagtatcg cggtttacgc cgaaaaagag
      901 gctaaaaata ttccgtggaa agcgaaaggg gcagaaatca ttgtcgaatg tactggcttt
      961 tatacctccg ccgataaatc gcaggcgcat cttgatgctg gtgcgaagaa ggtgttgatt
     1021 tccgctcctg ccggtgaaat gaaaaccatc gtttataacg ttaatgacaa cactctggat
     1081 ggcaacgaca ccattgtttc cgtggcgtca tgcaccacta actgccttgc gccgatggcc
     1141 aaagccttgc atgacagttt cgggatagaa gtcggcacga tgacgaccat tcatgcctat
     1201 actggcaccc agtcactggt ggatggcccg cgtggtaaag atttacgtgc ttcacgcgca
     1261 gcggcagaaa atatcattcc ccacactacg ggggcggcaa aagccattgg tctggtgatc
     1321 ccggaactga gcggcaaact gaaaggtcat gcgcaacgcg tgccggtgaa aacaggttcg
     1381 gtcactgagc tggtgtccat tctcggaaaa aaagtgactg ccgaagaggt gaataacgca
     1441 cttaaacaag caaccaccaa taacgagtca tttggttata ccgatgaaga aatagtctct
     1501 tccgatatca ttggcagcca tttcggttca gtgtttgatg ccacgcaaac ggaaattacc
     1561 gccgtgggcg atttacaact ggtgaaaacg gtcgcctggt acgataacga atatggcttc
     1621 gtcatgcagc ttattcgcac cctcgaaaaa ttcgctaaac tctgacgcgc acaggcggag
     1681 gaaaaaacct ccgcctcttt cactcattaa gactgtaaat aaaccacctg ggtctgcaga
     1741 tattcatgca ggccatgttt accatctgcg ccgccaatac cggatttacg ccatccggcg
     1801 tggaaacctt gcatagcttc gaagttttca cggttgatgt aagtttcacc aaacttcagc
     1861 cctttaatgg ctttcatcgc gacgttcaga ttttgggtat agattgatga ggtcaggccg
     1921 taatcactgt cattagccat tgagatagca tcttccagcg tgtcaaatgc gacaactggc
     1981 agcaccgggc caaaggtttc ctcatgcata atcgacattt cctggcgaac atccagcagc
     2041 aatgtcggcg gataataata tcctttcccc tctaccgctt tgccacccaa cgccactctc
     2101 gccccttctt ctactgcgcg cgccactttt tgctcaaccc tttccagcgc cgcggcgtta
     2161 atcaacggcc ccatcgcaat gtcgttgcgt tcagcggggt taccaaattg aaccgcctgc
     2221 aacgcttcac ccagccgatt gacgaactga tcataaatgc ctttctgtac ataaacacgt
     2281 tctgcacagt tacacacttg cccactatta atgacgcgtg aatcaacgat ggctttgact
     2341 gccagttcaa gatcggcatc gtccattacg atagccggtg ctttaccacc caattccaga
     2401 cacactttgg tgatgttttt cgccgcagtc gccatgatct tctcacctgc agagacgctg
     2461 cctgtcatac tgaccattgc gacctttggg ttacccgcca gttcttgccc aacggtttca
     2521 ccacgcccca gtacaaggtt aaacacgccg cgcggaaggc ctatttcatc gacgattttg
     2581 gcgaatgcaa tcgcattgtt tggcgtaaat tcactgggtt taatgacgat ggtattaccg
     2641 gtcaaaagag cgggagccat tttgcgggca atgaggaaga acggaaagtt ccacggcaga
     2701 atgccggtag tcacaccaag cgcacgttta aacagaagaa tattttctcc tggacgatcg
     2761 ctttgaataa tctcgccctc gtaacgccgt gcccactccg ccatgtaatc gatatagtcg
     2821 gcagtaaaag ccacttcgac ttcagctagc tgctggatct tgcccccttc ttcaacaatc
     2881 agcgcactga tttcactggc gcgttcgcgg atcccggcgg agattttgca caaccaactg
     2941 gcgcgttcaa tagcaggcaa cgcttcccat tctggttgtg cacgttctgc tgcatcgatt
     3001 gccttacggg catcctcggc ctgaccatcg ggtatgcggg aaatgacagc ctctgtagca
     3061 gggtttacca catcaatcca tgcgtctcca cgccaggtaa caaactgtcc atcgatatac
     3121 ataggatgtt gaacgggtac tgacatgggc g
//
LOCUS       DAJTNM010000227         3132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTNM010000227 DAJTNM010000000
VERSION     DAJTNM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.229"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..144
                     /locus_tag="OO172_004107"
     CDS             <1..144
                     /locus_tag="OO172_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCR5514044.1"
                     /translation="TIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFK
                     EVK"
     gene            complement(185..655)
                     /locus_tag="OO172_004108"
     CDS             complement(185..655)
                     /locus_tag="OO172_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5514045.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(702..1421)
                     /gene="yehT"
                     /locus_tag="OO172_004109"
     CDS             complement(702..1421)
                     /gene="yehT"
                     /locus_tag="OO172_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5514046.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1418..3103)
                     /gene="btsS"
                     /locus_tag="OO172_004110"
     CDS             complement(1418..3103)
                     /gene="btsS"
                     /locus_tag="OO172_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5514047.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          805 a          825 c          731 g          771 t
ORIGIN      
        1 actatcgata tggaaaaagt ggattttaaa gccctgcagg gtatttcagg aatcaacgtt
       61 tctgctgaag atgccaaaaa aggtatcact atggcgcaaa tggaactggt gatgaaagcc
      121 gctggtttta aagaagtgaa ataatctgtc ggcggccatg tttcgcatgg ccgccatacc
      181 cgtcttagct tttcttcaca tgctggcgcg ctgccagtcc acgcagaaaa taacgtaaaa
      241 attgatcgcc gcattcgcgg aagtttttat gatccggtgc gcgcatcatc gctgtaattt
      301 ccggcatcga aacgcggaac tgctgttcgg tgaggatagc cagaatgtca tcggttttca
      361 gcgaaaacgc gatgcgtaat tttttcagca cgatgttgtt attaatgcga cgttccggct
      421 ccagtgccgg agcagactca tccttgccgc gtttttcata aatcaggcca ttgaggaatg
      481 acgacaaaac aatgtccgga caacgctgaa aaccctcttc gtcttcttta cgtagccaga
      541 cggcgatctg ttccgcggtg gcttcgacat tatccagcgc cagaatacgc accaggtcat
      601 tattattggc tttcaaaatg tagcgcacgc tgcgcagaat atcgttactt atcatgaggc
      661 cttcaggtgt tgatgaggca aaaagccatt ttagcagtct tttacaggcc aatcgcctct
      721 tttaagcttt tcagataacg gcggctgacc ggcacggtta agccattacg caaaatcaac
      781 tcggcctggc cgttatcttc cagacgaatc tcctgtaaat gcgcgaggtt aaccagatac
      841 tgacgatggc agcgcagtag tggtgtacga ctttccaggg tacgtaatgt caattcggta
      901 aagccctctt tcccttcgtg gctggtaacg tagacaccgc tcatccgact gctgacaaat
      961 gccacatctt tcatttgcag caaataaatc cgactatgcc ccgtacaagg gataaatttc
     1021 agcgcctgtt gattttccgg taacagcgaa acatcctgct tgctgcgctc ctgacgcaat
     1081 cgcgccagcg ttttctccag tcgcgcttca tcaattggct tcagcagata atcaaaggca
     1141 tgttcttcaa aggctttaat agcgtattcg tcaaacgcag tgagaaaaac aatatacggg
     1201 cgatgttccg ggtcaagcat ccccaccatt tccagaccac tgatgcgcgg catctggata
     1261 tcgagaaaca gcacatccgg gcgcagttta tgcaccgcgc cgatcccttc cacggcgttt
     1321 gaacactctc caacgatttc aatatcgctc tgctcctgca aaaatatacg caggttctcc
     1381 cgtgctaacg gttcatcatc gacaattaag actttaatca tgcctcgtcc ctccatggta
     1441 gtcgtaacgt tattcgggtg taactatcag gctcacaggc gacgcttatt ccatagtcat
     1501 cgccaaaccg ttcacgtaaa cgcttatcca ccagattcat ccccagccca ctggcattgg
     1561 ttaccggttg atacaaaccg gcattgtctt cgatctccag catcaaatgt tgcccctcac
     1621 gtcgggcgct gattgccact cgccctgtat ccagcagttg tgatgtccca tgtttaatgg
     1681 cgttttccac tatcggttgc agggtaaacg cgggcaattg ctgctgggat aattcttgcg
     1741 gaatagcaat gttgacctgc aaccgcgact ggaagcgcgc cttttcaatt tgcagataag
     1801 cgttcacatg ttcaatttcg tcggcgagag taacaaactc cgaaggccgc tttaagtttt
     1861 tgcggaaaaa agtggaaaga tactgcacca gctggctggc ctgttcgctg tcgcggcgga
     1921 tcaccgcttt aatggtgtta agcgcattaa acaaaaaatg aggattcacc tgggcgtgaa
     1981 gcagtttgat ttctgactgg gtgagcatcg ctttttgccg ctcatattgc ccggcaagaa
     2041 tctgcgccga aagcagttgc gcaatcccct cgcccagcgt gcggtttatt gaactgaata
     2101 aacggttttt ggcttcatac aatttgatgg tgcccatcac gcgctgattt tcgccacgca
     2161 acggaattac cagagtcgac cccagtttgc attgcggatg caaagagcaa cgataaggta
     2221 cttcgttgcc atcagcgtag accacttcac cggtttcaat cgcttttaag gtgtaagtcg
     2281 aagaaatcgg tttgccgggt aaatggtggt cgtcaccaat tccggtaaag gccagcaatt
     2341 tctctcgatc ggtaatcgcg actgcaccaa tatccagctc ctgatacagc acctgagcca
     2401 ctttcatgct gttcacttcg ttaaacccct gtcgcaaaat gccttccgtc gaggctgcca
     2461 ctttcagcgc agtggcagaa aaagccgaag tgtatttttc aaacatcgcg cgtttatcga
     2521 gcaatatacg cataaacagc gccgcgccga cggtattggt gaccatcatt ggcgcagcaa
     2581 tattactcac cagacgcacc gcatcttcat aaggtcgggc gatcgcaagg atgatcagca
     2641 tttgcaccat ttcagcgacg aacgtgacgg caccggcggt aatggggtta aagactttat
     2701 cagtgcgccc gcgacggatc aggatgctgt gtaccaggcc gccgagcaat ccttcaacga
     2761 tggttgagat catgcaactt agcgcggtca tgccccccat cgaatatcga tgtaagccgc
     2821 cggtcagacc aaccagccca ccgacgaccg gaccgccgag taagccgccc attaccgcgc
     2881 ctatcgcacg ggtattggca atagaatcgt caatgtgcaa cccaaaccag gtgcccatga
     2941 tgcagaagat ggaaaagacg atgtagcaga gaaatttatg cggcagacga accgtgacct
     3001 gcattaacgg tatgaataat ggcgttttac tcattaacca cgcaatgact aaaaaaacgc
     3061 acatctgctg aagcagcagc aacaccagat taaaatcgta catacccgca aaccacactt
     3121 ccctttaaaa cg
//
LOCUS       DAJTNM010000228         3081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTNM010000228 DAJTNM010000000
VERSION     DAJTNM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.230"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..679)
                     /gene="ipaH9.8"
                     /locus_tag="OO172_004111"
     CDS             complement(<1..679)
                     /gene="ipaH9.8"
                     /locus_tag="OO172_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5514048.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQR"
     gene            complement(941..1036)
                     /locus_tag="OO172_004112"
                     /pseudo
     CDS             complement(941..1036)
                     /locus_tag="OO172_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1072..1377)
                     /gene="ccdB"
                     /locus_tag="OO172_004113"
     CDS             complement(1072..1377)
                     /gene="ccdB"
                     /locus_tag="OO172_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5514049.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1379..1597)
                     /gene="ccdA"
                     /locus_tag="OO172_004114"
     CDS             complement(1379..1597)
                     /gene="ccdA"
                     /locus_tag="OO172_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5514050.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2133..3081)
                     /locus_tag="OO172_004115"
                     /pseudo
     CDS             complement(2133..3081)
                     /locus_tag="OO172_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          768 a          750 c          741 g          822 t
ORIGIN      
        1 atctttgcag ggcttgcaga gcatgggatg ataatgggtt atcactaata tgtattgaac
       61 attcattcct cagattaaga atgctttccg ggatatgact tatctgattt ctatcaaaaa
      121 aatactctct gacaacggga ttatttccct cagaaaatgc tggtagctca gtgaggaaat
      181 tacgggtcgc tctgagattt ttaagagcct gtgggagcga tggtaaggag actatttcgt
      241 tatagctgat attcatagtc aataacgaat cgggtaaagc tggcaggttt tccagctcat
      301 tgtgttgtac ctgaagtgac tccagcgcag gaggtagcac gggcaattct gataatttat
      361 tgctggcgga atataatttt tttagcgtaa caggcagttc aggtaggtta gttaattgat
      421 tatatgatac attgagcagc gttatctgag ctggtaagtt gtcaggtagc gaggacagat
      481 ttaaacggtc cagtcgaagt tcatcggaat tattgataag acattcttta agtcgggaga
      541 cagcctcatc acgctcttca ccggggagcg cttgtttttc ccatttatcc catgctgaaa
      601 agtaatcagc atatgtaccg gaaatagtgt tataaaaaga attttggggc aatgaaaagt
      661 tattatttat cggtaacatg atgggcatcc agttaaagcc ggtggacaaa tggctttgtt
      721 aaaagaaata agtaggagtt tttatgaagt tcattccatg aaatgaactt cagcactttt
      781 ttattataat aaggataaac aaaattggct gtaaaaaaac gatactgttt cagttaaccg
      841 tgaatggcct gtttgcaccg tattgttctt gtttttcggt tagttaaaca tcgtacttca
      901 tatttgaacg gtaagcggct cgtcagaacc gtattgatat ttactgagag gtcagatcaa
      961 ctttccaggg caacagatcg cgtacccggt ttgccggcca gtcctggata tgctcaatga
     1021 cgtaacgcag ccattttgtc aacgccacga tttttgaccc tagcctgaca tttatattcc
     1081 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac
     1141 ttcttccccg ataacgaaga tcggcacact ggccatatca gtggtcatca tgcgccagct
     1201 ttcatccccg acatgcacca ccgggtaaag ctcacgggag actttatctg acagcaggcg
     1261 ggcactggcc agggggatca ccatccgtcg tccgggcgtg tcaataatgt cactctgtac
     1321 atccacaaac agacgataac gactctctct tttataggcg taaaccttaa actgcatttc
     1381 accagtccct attctcatcg gcaaaagagc cgttcatttc aataaaacgg gcgatctcag
     1441 ccatcccctg ctgattttca gcctgccagc gttccgcacg cagacggcgg gcctcttttt
     1501 gcatggccgt atttaccagg cctgagatat tcacgtttgc ggatttaagt aactgatagc
     1561 tgtcactgtc tatagtgaca gtaatacgct gcttcatgat aagccctcaa aaacatactt
     1621 atgatatgta tatgagtata tattaatggt acaaaccatc aatatccata aaaagtacac
     1681 cattaattcg ctataagttt ttaatacaat tttttctgct gcgatttttt tatactataa
     1741 ttagcatact tataacaccg taagtaaatt acatgaaagc aggcacagcg tgatccgatt
     1801 tttctgcgta taacgatttt ccggaagtgt aatacggttg cgccccgggc cgggcaccct
     1861 tttctgttgt tgtgtacggg cccacagaga atggtaccgg aggcgggctc agaagtactg
     1921 agtcgggtga tcgcgtcagg ccgcaaagta ctgacacggg tattggtgac tggtgatgaa
     1981 gtactgcccc gggtatgatt tcaggctgat tcaggttctg attctctgtc agtccgcgtg
     2041 gttgctggcc tggctgattt cctgttgtca ggcgttcaac aaccgactgc aggttatagc
     2101 ccggttcacg aacacctgac ggcaggtatc actcacccgg tgaaggtaaa tccctcaaga
     2161 ggcagcaact ctgccagtcg ctcctccggc cattccggca gacgcgtcag gacgtccgtc
     2221 agccaggcat gtggctccag accattacgt tttgctgttt ccagaaggct cattatctgt
     2281 gccgcccggt ttccggccat cagtgaaccg gcgaacagcc acgctttccg tcccataaca
     2341 accgggcgaa tggcacgctc gcagatattg ttgtccaggg gcaccgctcc gtcacccagg
     2401 aagcagctca gttcatcgcg ccggttcaga atatagttaa tcgccttatt taatggcccg
     2461 tcgggcggac agcacggctc ctgctcttca agccaggcaa acaggtcatt cactatcggc
     2521 cgggagtacc gttatcgcca ctgccgtatt ttttccacag ggcgctcacg gatgagtttc
     2581 tcaatgcggt aaagacccgc gatttttctc agctccacgc ctgcacgggg ctccctgctg
     2641 gccttataca gatttgcgaa gccgcgccct gcgtgactcc agcaacaggc tgatgtgatg
     2701 ccggggtgat tatccgccag agatttgtac acgctgtatc cgtcactcac cagcgtaccg
     2761 ccacaccagc actgcagcca ggtctcaggg tatctgaggg cccgtccggt ctgcgagtca
     2821 aaacagacga ccggcggacc gcttctttca ccgctgacgt aagcacacag ccagccggag
     2881 caggatttac cgcctttccg ggtatcaagg agcctcaggg acgtttcatc tgcatgaata
     2941 acatcccgtg tcagaagctc atggcgtagc agtttcgcca gcggggccag tgcagctccg
     3001 gcaacaccga ccatatccgc cattgtactg acggggagct ccacacccat tcgggagaaa
     3061 atgtgctgct ggcgatacag g
//
LOCUS       DAJTNM010000229         3065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTNM010000229 DAJTNM010000000
VERSION     DAJTNM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.231"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..342
                     /locus_tag="OO172_004116"
     CDS             <1..342
                     /locus_tag="OO172_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5514051.1"
                     /translation="AKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRR
                     GIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIAR
                     VRSVEEILHAL"
     gene            697..945
                     /locus_tag="OO172_004117"
     CDS             697..945
                     /locus_tag="OO172_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5514052.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1061..1330)
                     /locus_tag="OO172_004118"
     CDS             complement(1061..1330)
                     /locus_tag="OO172_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5514053.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1387..2055
                     /locus_tag="OO172_004119"
     CDS             1387..2055
                     /locus_tag="OO172_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5514054.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2371..>3065
                     /locus_tag="OO172_004120"
     CDS             2371..>3065
                     /locus_tag="OO172_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5514055.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          922 a          682 c          608 g          853 t
ORIGIN      
        1 gctaaagtgt tgcccgaaaa ctggtggcaa catccagctg cattaggtgc aactgacagt
       61 gatatcgaaa tcatcaaacg tcagtggggt gcgttttacg gtacggatct ggagttgcaa
      121 ctgcgccgcc gaggtatcga tacaatagtg ttatgtggga tctcgaccaa tatcggtgtt
      181 gaatccaccg cccgcaatgc ctgggaactt ggctttaatc tggtgattgc cgaagacgcc
      241 tgtagtgccg ccagcgccga gcaacacaat aacagcatta atcatatcta cccgcgcatc
      301 gcccgtgtgc gtagcgtgga agagatcctt cacgcgttat gatttacatc ggtctaccgc
      361 aatggtcgca tcctaaatgg gtgcggttgg ggatcaccag ccttgaagag tatgcccgcc
      421 actttaactg cgtgatacgg gatttttaaa aataactaaa gaacgcccaa gagcatgtgt
      481 tttctttaga ataatcaaag cgttgtattg ccatgtcata tgaaaatcgg caaagttcat
      541 atgtaacaaa gtgtcccaat catgccccaa aatgccccac aacaaatatt tttgccccat
      601 gcatgcccca aaaaatcact ttccctcact cttgacactg tttatccata cagttaaaaa
      661 taatactgta taaaaacaca gtgtagaggg atttttatgc gtattgaaat ctgcatagcc
      721 aaagagaaaa tgactaaaat gccaaccggt gctgtggatg cgttaaagga agaattaacc
      781 cgacgcatca gtaaacgtta tgacgatgta gaggtgatcg taaaagccac cagcaacgat
      841 ggcctttctg ttacacgcac cgcagataag gattctgcaa aaacatttgt tcaggagact
      901 ctgaaagata cctgggagtc tgctgacgag tggtttgttc actaataaac acgtcaaatc
      961 ggtaacggct ggaaatcatt caatactcgc actatcgaaa gtttaccagc caaccgcagc
     1021 acgtcctgca tacgtcgtgt ctgcggtttt tctttttcgc ttacattgtg tctggttctt
     1081 ccggccactc aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact
     1141 tcttccaggc ttccagcaac gagttttctt cctccgttgc gatctccaga tctacagcat
     1201 cctgaagtgg cgcaatatgc tcactgaatc cctggatgta gaactgtgtg gtgacggtct
     1261 tccagccatt cggctcctgc tgtatcgaag cataccaggc tatttcaata tcgctatgct
     1321 gcggcagcat ttaacccctt gtaattcatc gccataattg atttaattca caaataaaac
     1381 tataatatgg tgaaattaat gaaaaaaaac acagatgatg gggctaaaat ttacacacca
     1441 cttaccctaa agctttatga ctggtgggtt ttgggagtat caaatcggct tgcatgggga
     1501 tgtcctacaa aggaacacct tcttccacac tttctggaac atttaggtaa caaccatctg
     1561 gatattggtg ttggaactgg gttttacctt actcacgtac ctgagagtag tctgatatct
     1621 ttaatggatt tgaacgaagc tagcctgaac gcggcatcta caagggctgg ggaatcaaaa
     1681 attaaacata aaattagcca tgatgttttt gaaccttatc ctgcggcgtt acatggtcaa
     1741 tttgattcca tttccatgtt ttaccttctt cactgcctgc ctggaaatat atctacaaaa
     1801 agctgtgtaa tacgcaatgc ggcgcaggcc ttaactgatg atggaactct atacggagcc
     1861 acaattcttg gcgatggagt tgtgcacaat agcttcggtc aaaaactgat gcgcatttac
     1921 aatcagaaag gcatcttttc aaacacaaaa gattccgaag aaggcttaac acatatactc
     1981 tcagagcatt tcgagaatgt taaaaccaag gttcaaggta ctgtagtaat gttttccgct
     2041 tcagggaaaa aatagcatcc aaccgcagca cgttcttgct taagacgtgc tacggtttcc
     2101 tttacgattt ggggttgcga ctttacctct attgataatg cattccggca gaacgttcaa
     2161 atatgaacgt acgatattta actaaccgaa aaacaaaata tagcacaggc gagacattta
     2221 ccatctaaga aaatagtatc gtttttttac agttaaatca acatcacttc cttaaaatga
     2281 aaacaataaa tttcaatcct gaaacatttt atttcagctt attattcccc agtatcacat
     2341 tttatcagga tatccttctg cgaggttatt atgcttcctg taaataatcc ccccctatcc
     2401 actggaaacg tctcttttta cagaactaca tcaatcgaca atgttcacaa taattatctc
     2461 tccgaatggg ttgaatggac taaaaacagc atttccggag aaaacaggga aactgctttt
     2521 acccggctcc aattatgtct ggagaacagt gaaacatcgt tggacttatc ttgtttaggt
     2581 ctcagatctc taccacgatt gcctgacaat cttgatgaaa ttaatgtaag caataaccaa
     2641 ctatcaatgc tccccgagct accaagggca ttgaaagagc tgaatgcaag cagtaatcaa
     2701 ttatctgcac ttcctgaatt accagtgtcg ctggaatata taaatgtgag tgataaccat
     2761 ttgttcgcac ttcctgaatt acctgcgtca ctagaatata ttaatgtaag tgacaatcac
     2821 ctgtctgtac ttccgaggtt accaatgtca ttggaattac ttgatgcagc cagaaatgct
     2881 ttggaagtaa taccagagtt tccagaaaga gatgatcata ttataagaat attctggctt
     2941 aatcagaacc ggatcacggc aattccggaa agcatacttg gcctcagttc tgatagcgtt
     3001 gtcaatctta gagaaaatca actatctccc agaataatgc aaactttgtt acaacaaacc
     3061 gccca
//
LOCUS       DAJTNM010000230         3017 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTNM010000230 DAJTNM010000000
VERSION     DAJTNM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.232"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            281..1894
                     /gene="mppA"
                     /locus_tag="OO172_004121"
     CDS             281..1894
                     /gene="mppA"
                     /locus_tag="OO172_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5514056.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1945..2976)
                     /gene="ynaI"
                     /locus_tag="OO172_004122"
     CDS             complement(1945..2976)
                     /gene="ynaI"
                     /locus_tag="OO172_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5514057.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT          867 a          663 c          702 g          785 t
ORIGIN      
        1 caacgttttg gttatttttt attcttcgta caatggcgac agatgctgat tatgataccg
       61 aaaactggtt tgaacgtgcg aagcccgaac gtagtgttcg gtgttctatg tgctttgccg
      121 cattttggcg tctattattt acactaaatc tgatttgata tattgatact taaaacattt
      181 gatgcttcct ttgtcacttt tttgatggaa gttgtttgca tttctttaag gcgaaacaaa
      241 taattacgca tcaattttaa tgtcggttag agggaaactt atgaagcact ctgtttcagt
      301 tacgtgttgt gcgctgttgg tcagcagcat ttctctttcg tatgctgcag aagttccgag
      361 cggcacagta ctggcagaga agcaggagct ggtgcgccac attaaagatg agcctgcgtc
      421 gctggatccc gctaaagccg tgggcctgcc agagattcag gtcattcgcg atctgtttga
      481 aggtctggtg aatcagaacg aaaaagggga gattgtcccc ggcgtcgcga ctcaatggaa
      541 aagtaatgac aaccgtatct ggacttttac cctgcgcgat aacgcaaaat gggcggatgg
      601 cacaccggta acggcgcaag attttgtcta cagctggcaa cgtctggtgg acccaaaaac
      661 attgtcgcca tttgcatggt ttgccgcgct ggcgggaatc aacaacgcac aggcgattat
      721 tgatggtaaa gctacgcctg atcagcttgg cgtcaccgca gttgatgccc atactttgaa
      781 aattcagctt gataaaccgt tgccgtggtt tgtgaattta accgctaact ttgccttctt
      841 cccggtgcaa aaagccaacg tagaaagcgg taaagagtgg acgaaacccg gaaatctgat
      901 cggcaatggc gcttatgttc ttaaagagcg cgtagtcaat gaaaaactgg tcgtggtacc
      961 gaatacccat tattgggata acgccaaaac ggtgctgcaa aaagtgacct tcctgccaat
     1021 taatcaggaa tccgcagcca ctaagcgtta cctcgcgggg gatattgata tcaccgaatc
     1081 cttcccgaaa aatatgtatc agaagctgtt gaaggatatt ccggggcagg tttatacgcc
     1141 accgcagctc gggacctatt attatgcctt taacacgcaa aaagggccga cggcagatca
     1201 gcgcgttcgc ctggcattaa gtatgacgat agatcgccgc ctgatgaccg aaaaagtgtt
     1261 ggggacgggc gaaaagccag cgtggcattt tataccagat gttaccgccg gatttacgcc
     1321 ggaaccttcg ccgtttgaac aaatgagtca ggaagaactg aatgcgcagg caaaaacttt
     1381 gttgagcgca gctggttatg gtccgcaaaa accgctgaag ctgacgcttt tgtataacac
     1441 ttcagaaaac catcaaaaaa ttgcgattgc tgtagcatcg atgtggaaaa agaaccttgg
     1501 cgtagatgtt aaattgcaaa atcaggaatg gaaaacctat atcgatagcc gtaacaccgg
     1561 caattttgat gttatccgcg cctcatgggt gggggattat aatgaaccct ccactttcct
     1621 gacattatta acgtcaatgc attcaggaaa tatttcacgc tttaacaatc cggcatacga
     1681 caaagttctg gcccaggcat cgacggaaaa taccgttaaa gcgcgtaatg ccgattacaa
     1741 cgcggcagaa aaaatcctca tggagcaagc accgattgca ccaatttatc aatataccaa
     1801 tggacgatta atcaaaccgt ggctgaaagg ttatcccatt aataaccctg aagatgtagc
     1861 gtacagtcgg actatgtata ttgtgaagca ttgatgtgat gggaactggc gttacccttg
     1921 tggataacgc cagtgatgtc tgatttagcg accctgttcc ggcggtgtaa tgttatccat
     1981 atacagcgtc tggctgggga aggcaaagtc cgcgccgtgt gactgtacaa tatcgataat
     2041 cttcaaataa acgtcttgct gtgcagcaag ccactcagcc cataccgtgg ttttggtaaa
     2101 gcaataaacc ataatattca atgacgagtc agcaaactgg ttgaaataaa ccagtaaggt
     2161 ttgtcgctgg tcgatggccg ggtgattttt cagcatctca cgtacggctt cgacaataac
     2221 gcccactttt gccgcatcct cataacgtaa accaatggtc gtggtaatgc ggcggttggt
     2281 cattcgtcct gggttttcta cgctgatcga cgaaaacagc gagttcggta cgtacaatgg
     2341 acgattatca aaggtcgtaa ttttggtaat tcgccagcca atttccgcta ctgtaccttc
     2401 gatatttctg tccggtgaac ggatccagtc gccaatactg aaaggacggt cgaaatagag
     2461 catgatcccg gaaaagaaat tactcagaat atctttaccg gccataccga cagccagacc
     2521 accaatacca ccaaaggtca gcaagccaga aaggctcatg ccgaaatgtt cgccataaag
     2581 cagaacaagc actacaataa tggtgatttt gataatgcgc gacataatcc gcgcactggt
     2641 gatatcgcga ccttttttaa tctgctgttt ttcaaactga ttaatcagca gaaatagctt
     2701 aatcgtcaga ataaccgcaa tcagggacgt acagataaaa tcgataacgc ctggggtgat
     2761 aaatttgagt ttatagtttt ctataacata attaatgatg ctaccaacag cactgataat
     2821 tatggtgtag attaaaaatt gcaccgcatg gaataaaaat ccttttcttt tacgatttcc
     2881 acggcgaaac caaaagctca tcagaatcaa tgctgcgcag ctaccgaaaa taatgaccag
     2941 attaagcgca ttatttgtaa acagttcagc gatcattgtt ttatcaggct cctccagata
     3001 attgtcgtca tgccgga
//
LOCUS       DAJTNM010000231         3014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTNM010000231 DAJTNM010000000
VERSION     DAJTNM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.233"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(168..2009)
                     /locus_tag="OO172_004123"
     CDS             complement(168..2009)
                     /locus_tag="OO172_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5514058.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2009..>3014)
                     /locus_tag="OO172_004124"
     CDS             complement(2009..>3014)
                     /locus_tag="OO172_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5514059.1"
                     /translation="PEAMQSWLQNNANELARVGVDPNSVAQMYQQNPSGFGEFVDHLG
                     MAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQARGQNLSYQSAMTGHNIA
                     AQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGIETAVNNATASSNSANSMS
                     ALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQNALVNSQVLKFLPPGPAT
                     DKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNEFKAEWMSANGNPGQSRNG
                     GQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEPRNQVGSYTSKSGIQFTVE
                     "
BASE COUNT          679 a          757 c          656 g          922 t
ORIGIN      
        1 gatacgatca cattggctgt aatgtctgtc atttaatttg ctccagatac aatgaatcgc
       61 cgcagcgtgg ctacggtgaa ttttgggcat aaaaaaaacc agccgaagct ggatagttgc
      121 gttggttatc tgtcagtagt tatgtgctta tttaattatt tatctatcta ttgttctgct
      181 gtaatttggc ttgaaagtat agggcgtatc gcatttgcag cattatttaa cgctctttca
      241 taagctgccg ttcctgcttt ggtgtttgcc aaacgaagaa gcatattcct tgcggctttg
      301 gactcataca aacgcatcat tgcaccaaaa ccagcctcaa gccccattga tacaccaaga
      361 gtcgcagttg cgccaatcgt ccttatcctg ttggcttgcg attgccccgt ctgagttact
      421 acatttgcgg tgtctgacct tgctgtttgc tgtagaactt catgaagagc atcaagctct
      481 ttcatgtgct ttccagaaaa aatagtgttg taaatttcac cgcctgactg agatttcagc
      541 ttattaactt cagtgatgaa cttggctgga gagtcaccgg ccttttccgc tattttgctg
      601 acgtaagctg cacgcatagc atctttccct ttatcatcca gtgcgctcca gattcgtttc
      661 acgtcagatg gttttctgct taatacaacg gtatttataa gttcaggact ggcttcactg
      721 cttgccttgt tgagcttgtt agcaatgttt ttattaagca ccttattata aacgtttgca
      781 taatcggaat ttgctttaag gtattttgct gcgtctgatg caccgaggtt tttagcaact
      841 gcgttacgaa ggtcttttga cattgcattc tctaccatat tggtagctgc ttttgcctgg
      901 ttggggaaga ccatagcatc tccctgaaca ttagatctaa atgctgttct gtgctgacgc
      961 aagagatcaa acgtaacatc caaatcagtt gcagggtttg ctaattcttc acgtaggtta
     1021 cgcaaggatg taagcaggct ttgattggca gacgtcccaa gccgttcctg tcttgcgatc
     1081 gctgtattca gagcattcat ggtatttgtg gtatcaactg cggcattacc cattttattg
     1141 gtgacgtcat tgataacagc gccagcggca tccttccgcc cccttaacgt ggtggtaaga
     1201 gatttcacca catcatcagg gttgtactca ccaaaacggt caaaataatt acttaccagc
     1261 ttactacgcg ttgcatattg ctccgctcgc tttgagcctg tcccgagcaa agccccctcg
     1321 gcatcctgag ttaggccgcg agtgaaagca tttttcggcg ggataacatc agatgtcatt
     1381 ggtgtcacgc ccatcgattc tgatgtggca attttcttcg ccacttctgg cgcaatatca
     1441 ccttttatag ccgttattcc acgccctatt ccctttgctg ctgcggaaag aacaccctga
     1501 gcggcaaggt taactccggc atttttagct gcattttgtg cgaaatcgcc tttctgattt
     1561 gcggcctctg ccagtgatcc aatagccatg cttcctgccg ttccaactcc tggaactaaa
     1621 tacccaccaa ttgtttcacc ggcttgcgcg taggggtctg tcggtctgtc tactggacga
     1681 taaacatcat ccaaaacctt gggtccacca agcccctgac tgattgcatt aatcagactt
     1741 gcgccgccct gcaatacgtc aaatggtatg tttaccagac cacgaccagc ctgttctgca
     1801 atttgccctg cactttgacc accagtgagc caatcgccag cttgttgcat caatgatggt
     1861 tcttcccgtg ttggtgcatt attggcctga ttaactgttt gttgctgaac agcctgacca
     1921 gcaaaatact catcaatggc ggtgccaata tcttcggtgc tcgtaccatc aggaaaggta
     1981 aatgtcttac cgtttgcagt tactttcatc attccaccgt aaattgaatg cctgattttg
     2041 aggtatatga tccaacctga ttccgtggtt ctcctgaagg tgtcgaatct tgtgctggcg
     2101 ctgcgtcagt attcattgac atataccgct taacggcact ccccaatgat tcaccttttt
     2161 taacatccaa ccccaatatc tgaccgccat tacgcgattg tccagggttg ccattcgcgc
     2221 tcatccactc ggctttaaac tcattaaact gcgcgtttcg tcgctcaagg tttgccattg
     2281 catcaagcca tcttgcgacc gtctcagggt tatccatgtc agttggcgca ccctgtcgaa
     2341 cgatctcaac gtctttatcc gttgctgggc cgggaggtag gaatttaaga acctgactgt
     2401 taacaagggc attttggcga atgcgcaaat cacgcaatgt tgtatcgctt ccggtaagtt
     2461 ttgcgaacat gttctgtgcg ttaccgaaca aacctgtcgt tggtttttct gctctgaact
     2521 gttgagcaag cgcactcata gaattggctg agtttgatga tgctgtagca ttgtttacag
     2581 ccgtctcgat gcctttttcc atgtttactg acagcttagg tgcttcgcta atcaactgct
     2641 gagccttttc ctgcgcttgc tgcatcttaa acccgaactc ttgctgatcc agagccaagc
     2701 gttgtgctgc gatattgtgc ccagtcattg ctgactgata ggaaaggttt tgccctctcg
     2761 cctgaagtgc ttctccagcc tgattgctgc ggattgtctc tgccagcctg cctcggtcaa
     2821 tttcacgacc agccatcttg tcctgaacat tgaagtaatc aatcggacca agagcagcca
     2881 ttccaaggtg atcaacaaac tcaccaaatc ctgaaggatt ctgctgatac atctgagcaa
     2941 cgctgttagg gtcaacaccg acgcgcgcca gctcattggc gttgttttgc agccatgatt
     3001 gcattgcttc tgga
//
LOCUS       DAJTNM010000232         2813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTNM010000232 DAJTNM010000000
VERSION     DAJTNM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.234"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..400)
                     /gene="intS"
                     /locus_tag="OO172_004125"
     CDS             complement(<1..400)
                     /gene="intS"
                     /locus_tag="OO172_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5514060.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEI"
     gene            complement(562..636)
                     /locus_tag="OO172_004126"
     tRNA            complement(562..636)
                     /locus_tag="OO172_004126"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(601..603),aa:Arg,seq:cct)
     gene            complement(712..1644)
                     /locus_tag="OO172_004127"
     CDS             complement(712..1644)
                     /locus_tag="OO172_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5514061.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMVIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1938..2693
                     /gene="mlaA"
                     /locus_tag="OO172_004128"
     CDS             1938..2693
                     /gene="mlaA"
                     /locus_tag="OO172_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5514062.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPVRNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          682 a          679 c          679 g          773 t
ORIGIN      
        1 gaatctcaag gccgccgatg atgggtaaaa tgtcgtcatc aaacattttt gcaagttcag
       61 tcgcataccc tactgaccag acttgcttct tgtgttcgta ccattccttg taaatcgcac
      121 taaatgaatt gttgttagac gaagcctttt tcgcctttac cggatcgatg ccagccgaga
      181 tgtctttcct cgcagtccat gctttatccc ttgcctcctg caaagtcata agcggatatt
      241 ttccgacggt caggattttc tccttaccgt caatcttgta gcgaagctgc catacctttt
      301 tcccggatac agggacataa aggtacaggc cattaccatc gagaaggcgg tatggttttt
      361 ctttcggctt tgctgcttca atctgcttaa cggtgagcat gggtaaaaat ccggtgggta
      421 aaattatttt atccactttt tacccgtcat ggagtgcggc tgtcaacgat ctgacgcgaa
      481 ccatgacgaa ctgtgaatat acggaaggct tgatattcag gggattttgc ggactggtac
      541 ggatgggagc gaactgataa atggtgtccc ctgcaggaat cgaacctgca attagccctt
      601 aggaggggct cgttatatcc atttaactaa gaggacaatg cggcatgagt atacccgcta
      661 atggactgcg gggtaagtac gctgccgctc gattgcttaa accctcgcca tttatgctgg
      721 gtttttatca tttttcttaa tgttttccgc acgttctgct ttttggcgtg cttctgcttt
      781 acgcttgttg ctcatgtcgt tacgaatctg tgcatgactc attaacgcga agataaaggt
      841 gccgccgcag atgttccccg ctaaagtagg tagtgcgaag ggccagatga aatcgctcca
      901 gtgcagcgta ccgttaaaca ccagatagag gatttcaaca gaaccgacca cgatatgggt
      961 ggtgtcaccc agggcaataa gccaggtcat caatataatc accacaatct ttgccgcacc
     1021 tgctgcagga aacatccata ccatagtggc gatcagccag ccggaaatga tcgcgttggc
     1081 aaacatctcg ctgggggtgt tcttcatcac atccatgccg attttgacaa atgcatcgcg
     1141 agtttcttca ttgaagatag gcatatattc aaatgcccac gcagcaatac cagtcccgag
     1201 aatattaccc agcagcacga ctccccataa ccgcataagt aagccaacgt tgctcattgt
     1261 cggtttttgc atgacgggta gtaccgcagt cacggtgttt tcggtaaata attgctggcg
     1321 ggccattatg acgataataa aaccaaaggt ataaccgaga ttctccagca agaagctgcc
     1381 cggcacacct tccagttcga catgaaatat cccttttgcc agtaacgaag cgcccatcga
     1441 cagacccgcc gcaatggctg accacagtag cgccattgcg tcgcgttcta gctctttttc
     1501 accatcctgg cggatatgct catgaattac catcgctcgg gaggggagtc ggtcttcatc
     1561 tatttctatt tttttgccgc gctctttttc ttcgctctca acttcaattt cgtcgctgtg
     1621 ttgatcaatt ttgtcgttgt ccatggtctc ttctcttgaa ttagcacgta tagctaaagc
     1681 gtagcggctt ttttgctcgc aactggcggg agttactctg aaaatgtaga aaaggttgcg
     1741 tttgcctttt tctgtttcta tagaatcaag tagcctacag ggcggcgatt accaggctat
     1801 gatcaaatca gcaaatcagg gcgtctggac atcagttgac gtgctgttac aatcgcccac
     1861 acctaaacag gcggatacgg tatcgttccg tcatggatgg caaactgcat aagccataaa
     1921 aaaacaggga gacatttatg aagcttcgcc tgtcggcgct tgctctggga actacgcttc
     1981 tggtggggtg tgcgagttcc ggtacagatc agcaagggcg ttctgacccg ttagaagggt
     2041 tcaaccgcac catgtacaac ttcaacttca acgtattaga cccgtatatt gttcgaccgg
     2101 tcgctgtcgc ctggcgtgat tatgttccgc aaccggtgcg taatggtttg agcaacttta
     2161 ctggcaacct tgaagaacct gcggtgatgg ttaactactt cttgcagggc gacccttatc
     2221 aggggatggt ccactttacc cgctttttcc tgaacaccat tttggggatg ggcggcttta
     2281 ttgatgttgc agggatggcg aacccgaaac tgcaacggac tgaacctcac cgcttcggta
     2341 gtacgcttgg tcattatggc gtgggttatg ggccttacgt tcagttaccg ttctacggta
     2401 gcttcacgct gcgtgatgac ggtggtgata tggcggatgg tctttacccg gttctttcct
     2461 ggctgacctg gccgatgtct gtgggtaaat ggacgcttga agggatcgaa acccgcgctc
     2521 agctgctgga ttccgatggt ctgctgcgtc agtcgtccga tccttatatt atggtgcgcg
     2581 aagcgtactt ccagcgtcat gatttcatcg ctaatggcgg cgaactcaaa ccgcaggaaa
     2641 atccgaacgc gcaagcgatt caggatgatt taaaagatat cgattctgaa taagaaacaa
     2701 ataaaaaagg tgagtcgcaa aactcacctt ttttgttgtt atccctcaat taccacaagt
     2761 aaaaggagat aaaccgacaa tgacccggac gtaatgatgt aaaaatactg atg
//
LOCUS       DAJTNM010000233         2771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTNM010000233 DAJTNM010000000
VERSION     DAJTNM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.235"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(96..500)
                     /locus_tag="OO172_004129"
     CDS             complement(96..500)
                     /locus_tag="OO172_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514063.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            complement(1052..1768)
                     /locus_tag="OO172_004130"
     CDS             complement(1052..1768)
                     /locus_tag="OO172_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514064.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSYKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            complement(2352..2771)
                     /locus_tag="OO172_004131"
                     /pseudo
     CDS             complement(2352..2771)
                     /locus_tag="OO172_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          830 a          611 c          528 g          802 t
ORIGIN      
        1 tgtttttttg ctggttgata ctgtttttgt catgtgagtc acctctgact gagagtttac
       61 tcacttagcc gcgtgtccac tattgctggg taagatcaca tctcatcgct tacagccact
      121 acaggacgtt tccgtatcct gtgaattatc atcccacgtg gactgcatat atttttaata
      181 cacacaaccc ctcttccagc cacatgcagt gtcgaggtgg tcccgcagat tccgtatacc
      241 cgacaccctg cacgggttcc aagtatctgt actcccggtg gtttatcaga gtaatagtag
      301 tcactgttac attcgaccca gctctttccc ccggggggat gcgggaccac tcggaaatac
      361 accccatatg gtccgtgcac attaatctcc gcatagcaat gttctgttga attacgtggc
      421 atagccagta aagtctcttt tgcagcagcg ggtaccgcat accacataca cattagtgca
      481 actatcagcg cagtattcat tttcattgtc actttccgtt actggtgctg tccgtcacag
      541 cacctcatgt cctgcggaca gggcaagctg accggccata acaactttca gttccatcag
      601 ggaagtggaa gctagacgcc ggacaatcaa atcccgccgg cggtacacct tcttcgcagc
      661 agagttgttc agtcaacatg aaagtgacag acagtgtcac cacgatgcag catcagtatg
      721 acggtgaatc gccggtcgta gtttttgttc tttgttttat agggagcttt ctgcatcagg
      781 catcgttcat cacggggtat tggtgcaatg ctcggcatca ctcagtccgg ctggtgattt
      841 gtgatgtttg gcaatttatc tgattacata atccggactg agtcccccct gagtgtttta
      901 ctgttccgag cacttattta gtaactttct tgcatagttg tttctggctt taattgtgct
      961 gccctaaggt ttgtttctca gtatgacagc tcaatacata tacttcgaac gcattatata
     1021 ttcatttaat tcactttgtg ggagaggatt attactttct gacataaaca tcattgattt
     1081 cataaaatcc cgactgttct gtattaaata ttaagaatct gttactgtca tctttatatg
     1141 aactagacaa aacgagccgt gaatcataac gagcaagaac ataataccaa ctattattat
     1201 aacaaagcat ctgatattct ttttttaatt gaggtttata ccagccaaca agaaaggaaa
     1261 aaagccaaaa ataaaccaaa aaaccagcca taattaactt aatgcggtgt tttctaacaa
     1321 aagggatatc tgaaaaacat gatacagaaa agatccgtcc atatatacga attattattg
     1381 aaataaccaa tgaactaata atgcatgaaa gtgataatgg aagtatgaaa tattgtttga
     1441 gaattaaaag ttcgaatatt acagggaaaa acagtagggt aatagcaaaa aatagtctaa
     1501 aaaaactcaa gtcttgtatt ttacttcttt tctttataag aagaaaaaat aaaacaccaa
     1561 tcccccaacc ggtaaaaagt atacccatta ctatgatagc atagagtaaa cttctggcaa
     1621 catcatccaa acctgcacca acaacccacc atggaaatcc atagtaaaaa gaggttcccc
     1681 agccataaga ataagcactg acccacccta cacatcccat ataggcgatg aataatgaag
     1741 aatttctaag caatgaattg tcattcataa gaaaccaact gataatgtaa aaaatattcg
     1801 agataaaagt tgatctttta atttttatgc taggaaatta acaataatat cattgttaat
     1861 gcatatttat catatcaaaa tactctgcag aatgctacta tcaaaaccag acaagtgaac
     1921 aaaacttggt caacatttag taacgctagt tggtaatgtc ttcaatcgat agaagtttct
     1981 ggtaatctac tggtattcaa acagttaggg ctcatacagc ttgctggacc ttccccataa
     2041 ccgcccgaac gtgaaggtct gatgtgttgc aagagagccg gatgcgggaa atctgcaagt
     2101 ccggttcgat gagagggacg ttgtttttac ttgttgttga tgaagacttt cattattgaa
     2161 cagaatgata atattgtcta aacaaaacat taaatctcat aattctttgt agaaaacgct
     2221 tttcttgaaa ttaaaaataa gtagaatcag cagtctcatt ataataacag acaatgtcat
     2281 gctctcgccc gttccggtaa taaacggagc gggctatctt tttggaatat ttatggccca
     2341 caatccagtt ttcataaata cgattcattc cattcttcgt tttttctgag cattgcgttc
     2401 ataatggtca gcaacttacg catacaggca accagtgcca cttttttggc gtttccggct
     2461 gcaagtaggc gcatgtaaaa cgcctttatc accagattaa accgggggcc tcaagcgccg
     2521 ccatgtacag agctgttctt ccccagcaca atcaccgaaa atggttcgtc gaccgcgcag
     2581 agttcccgag tcccggttta cctgagcaac accgacaagt gcgctgattt cacgtctcga
     2641 gaggctgctc gattcaggca tttccgccag caatgctgcg gcagtcatgg ctctaacacc
     2701 tcagacgttc agtaagctct ttaaagtaac tctgaatgtg cctgttcata tctgggtctt
     2761 ccccgatcat g
//
LOCUS       DAJTNM010000234         2498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTNM010000234 DAJTNM010000000
VERSION     DAJTNM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.236"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            146..1099
                     /locus_tag="OO172_004132"
                     /pseudo
     CDS             146..1099
                     /locus_tag="OO172_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1140..2135)
                     /gene="pgl"
                     /locus_tag="OO172_004133"
     CDS             complement(1140..2135)
                     /gene="pgl"
                     /locus_tag="OO172_004133"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5514065.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVASYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTATLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          689 a          613 c          561 g          635 t
ORIGIN      
        1 atggttacat taaggacaaa gcatctctaa tgactttagc tcaacgatta ataacatttt
       61 ttcattaatt taagttggct aaatgcagtt aaaattttta acgaccagcc ccccaaaatc
      121 gtcgcttcca ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt
      181 catactggca gagtccagtt catttaacaa tgccgctaaa ttactcaata ttacgcaacc
      241 tgcattaacg cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg
      301 tacaactcgc aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga
      361 attaataaag aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg
      421 tggtatggtg acattagcgt gtattccgac cgcagtattt tattttttac cgctggcaat
      481 cggtaaattt aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa
      541 taattgcatg gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt
      601 cacaaattca tccattgatt ttaccccact ggtcaacgag ccattcgtgc tggcctgtcg
      661 gcgtgaccat ccattggcca aaaagtagct tgtagaatgg caggagttag tgggttacaa
      721 aatgattggc gtacgttcct ccagcggcaa ccgattgttg atagaacaac agctagccga
      781 caagccctgg aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact
      841 agtggaggca ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta
      901 ttcttcgatt attggtatcc cactggtgga acccgttata cgtcggacat tggggattat
      961 tcgccgcaaa gatgccgtgc tttctccggc agctgaacgc ttttttgcct tgctgattaa
     1021 tttgtggact gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc
     1081 gttacaggag ataagttaat acaccggcgc aggagagact gcgccggtta aatcagcggt
     1141 tagtgtgcgt taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca
     1201 tgcagtagcc cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgccca
     1261 gcggcaatca gatacttgcc gctgtaatca acattgaagc cgcgcggctg ggtttccgtt
     1321 ggctggaagc cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc
     1381 agagtggcgg tacggtcgca ggcgtataaa tggcgaccat ccggggtgat atgaatatca
     1441 gccgcccaac gggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattcg
     1501 atattgccgt gcggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg
     1561 caatacgcat attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg
     1621 gtagtcactt ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg
     1681 caaatgcgat cttgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg
     1741 gcggaatggc aaccgtccag cccctcgact acatcgacga cgcccactgg caggccatct
     1801 tccagacgcg ttacgctcac gttacccgca ttgtaagaag ctacaaagac aaactgcccc
     1861 tggtgatcgg tggaaatatg cgtcggacta cccggcagca cagactctgc ggcaaaggtc
     1921 agtgcgccat cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca
     1981 acatagagat aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg
     2041 acaacctgtg tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg
     2101 ctctcagggc tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc
     2161 ccttatcttt catacatata acaacactta ctggataaaa aacagacaac aaaacacgat
     2221 aaaatacaat atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata
     2281 ttactaaaat attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat
     2341 tcagggagta tgactctccc cgcttttgtt tacctgtatc aacgaccgta aacactatgc
     2401 cataacaact tctggccaac ttcccattcg ctcaacaatc aactcacttc cggcacaaat
     2461 aaacgcatta cgttgtagat cggtattctg gtcatttg
//
LOCUS       DAJTNM010000235         2479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTNM010000235 DAJTNM010000000
VERSION     DAJTNM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.237"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..646)
                     /gene="yidZ"
                     /locus_tag="OO172_004134"
     CDS             complement(<1..646)
                     /gene="yidZ"
                     /locus_tag="OO172_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5514066.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(621..1796)
                     /gene="mdtL"
                     /locus_tag="OO172_004135"
     CDS             complement(621..1796)
                     /gene="mdtL"
                     /locus_tag="OO172_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5514067.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1928..>2479)
                     /locus_tag="OO172_004136"
     CDS             complement(1928..>2479)
                     /locus_tag="OO172_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5514068.1"
                     /translation="GSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          707 a          660 c          635 g          477 t
ORIGIN      
        1 aggtgtcgct ctgttcccag caaatgctga tatgcggata acgtaaaaag gtgtccagat
       61 tccacgtttc atgcagtgcc ggatgatctt tgcgtaacca gacgcagggt acatcactaa
      121 acagcacttc ataatcaatg gctaacggta gcgagcttaa cagctcccgc gagcgaggat
      181 ggctttcgcg accggaaaaa ccaatatcca cttcaccacg agtaatggca tctaaggaat
      241 cgtaatccca gttacgtagt ttgatggtcg cctgcgggta acgttggtag atccgtttcg
      301 acagcgcatt aagcatgatc atcatcagcg gcgattccgc cgccaactca aacttcaggc
      361 cgcgcggtgt ctggtggtgc ggtttatcca gcagcagatt gctcatttgc atccattccg
      421 ccagattttg ctccatgctg accattagct gtgtgggcga cagccccagc ggtgagttca
      481 caaagagcgg gtcgtcaaac cacgctctta actttgccag cgacttactc accgctgaag
      541 gggtcacgtt catccgcttc gccgctttgg tcacgctgcg ctcctgcatc agcagttgca
      601 gacagagcag cagattgaga tcaagcgtgg tgatggattt cttcatgagc ggcaacgggg
      661 cgtccaggcg cgacgaacat aatcagcaac aggctcacta tgctacaggc aatcagaatc
      721 ccgatcagca tattccatgc gccgatacca accaccgcgg ccagccaaat ccacagtgac
      781 gaaccgcaaa cctgcgcaat acctaaggtc gagctggcta cgcccgcgcg taatgaaaat
      841 ggtcctaacg cctgactcat cgctacgcca aaacctaccg agaaaccggc gcaaatcagc
      901 gtgataccaa acagagaaac cgcatgggaa ggtgaaacgg caagggtgat ccccgccgca
      961 aggaataaca cctgcgaggt gatcatcaac gtacgtggct taaaaattcc cagcgcaaat
     1021 ggcgtcgaga atgaaaaggt catgctgacg ccagcggtca gcgccataat ggtggcgtat
     1081 tctccgcgct caaaccccat gatttccatc agcaataccg gcgatgtatt gacgaaagtg
     1141 aggatcaccg aaacgctgag ggtggtgata acaacacggc tgaggaaaaa acgattaagc
     1201 agcgactcgc tattttctcg tggtttgtcc gaagcagcgg gggacgctgg gcgcgtttct
     1261 tttaaaataa acaaagacaa catcagtacc gcgatgccca tcattgccat cgcccagaac
     1321 agactctgcc acgggaattt aagcataatc agatgtccga gcactggcgc taacaccgga
     1381 atgatgcagg taataccgtt gagtaatgac agcactttag cccgacgtcg atcatccagc
     1441 gtgtcgcgca aaatggcgaa cgccaccacg taacaacagc ctgcgcccaa cccctgtaga
     1501 aatcggcctg caagaaataa cgtgctggtt tcagccagtg aacagaacac cgaggcaata
     1561 ataaatagcg ccgcgccggg tatggcgacc ggctttctcc ctgaacgatc ggccacttta
     1621 ccggcaaata gcatcgcagc tgccatcccc gccagatata cggagaacgc aatatgcaac
     1681 tgcgcttcgc tggcattgag atcggcggcg atgcgcggta aaccaacgag gtacatatca
     1741 atcccggcgg gataaagtaa aaccagggca aaactacaaa tcaaaaagcg ggacatgggc
     1801 taaagcgtcc tgatagtaag gtgacgcaaa gcatacgtgg tgaaggagga taaggcgagt
     1861 tgccatttgg acataagtga tttcctgaga ggcaagaagc cagcgaatgg ctggcttctt
     1921 gaaggattta gccaaattta ggtaacacgt taaagacgtt gccgaaccag cacaaaatca
     1981 cggttatacc aaagagaatg acaatcgccg gaataagatt gccgccccag accgtgaaca
     2041 tctgattggg aaacttcttg cgagctttga ttgcaagcac tgcgggaata atgaccgccc
     2101 agatggtggc gcacagcccg gcaccgccga tcccgtaaat aaagccgttc gggaagatca
     2161 gatacaacaa cgcaggtggc aggaaggtta acagcacggt tttgaaacgc ccgccgtggg
     2221 agttatcaat cttaaacagg tccgccagat aatcgaacaa ccccagcgtg acaccaaaga
     2281 acgaactggc aacagctaag ttagagaaca ccagcaggca aaactcgata atgccgtgct
     2341 gtttggtgcc gaggaacgat ttcaccagcg aatcaacgtt gccgcctgag gagattatcg
     2401 ccttaaagct ttcgcgcgga atattcccca tcgtgcaata gagccagaag agataaatca
     2461 ccagcgccag cagcgaacc
//
LOCUS       DAJTNM010000236         2336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTNM010000236 DAJTNM010000000
VERSION     DAJTNM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.239"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..72
                     /locus_tag="OO172_004137"
     CDS             <1..72
                     /locus_tag="OO172_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514069.1"
                     /translation="LVPTISGVHDLTETSRWIQTWPN"
     gene            60..506
                     /gene="etp"
                     /locus_tag="OO172_004138"
                     /gene_synonym="yccY"
     CDS             60..506
                     /gene="etp"
                     /locus_tag="OO172_004138"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5514070.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            526..>2336
                     /gene="etk"
                     /locus_tag="OO172_004139"
     CDS             526..>2336
                     /gene="etk"
                     /locus_tag="OO172_004139"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR5514071.1"
                     /translation="MTTKNMNTPLGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMLPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINTLQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQHQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQH"
BASE COUNT          623 a          559 c          612 g          542 t
ORIGIN      
        1 ctggtaccga cgatttccgg ggtgcatgac ctgacagaga cttcacggtg gattcaaaca
       61 tggcccaact aaaatttaac tcaatcctag tggtttgtac cggcaacatt tgccgttcgc
      121 ccattggcga gcgcttgctg cgtaaacgtc tgccgggcgt aaaagtcaaa tcggcaggtg
      181 ttcatggtct ggtaaaacac cctgcagatg cgacagcggc agatgttgcc gctaatcatg
      241 gcgtatctct tgaaggacat gctggacgta agctcactgc tgagatggca cgaaattacg
      301 atctgattct ggcaatggag tcggaacata ttgctcaggt taccgcaatt gctcccgagg
      361 tccgcgggaa gacaatgctg tttgggcaat ggctggaaca gaaagagatc ccggatccct
      421 atcgtaaaag tcaggacgca tttgaacatg tctacggtat gttggagcgc gccagtcagg
      481 aatgggcgaa gcgcctcagc cggtaatttg agtttataaa atacgatgac aactaaaaat
      541 atgaatacgc cactaggcag cactcaggaa aatgagatcg atctgcttcg tctggtcggc
      601 gagttatggg atcaccgtaa gtttattatc agcgtgaccg cgttattcac gctgatcgct
      661 gtcgcttact cgctgttaag cacaccaatt tatcaggcag atactctggt ccaggttgag
      721 caaaaacagg gcaacgccat tctcagcggc ctgagcgata tgctccctaa ctcatcgccc
      781 gagtctgcac cggagatcca actgctgcaa tcgcgcatga ttctcggtaa aaccattgct
      841 gaactgaatc tgcgcgacat agttgagcag aagtattttc cgattgtggg tcgcggctgg
      901 gcgagattaa ccaaagaaaa accaggtgag ctggcgatca gctggatgca tattccacaa
      961 ctgaatggtc aggatcagca actgacactc acggttgggg aaaacggcca ctatacactg
     1021 gaaggtgaag agttcaccgt caatggtatg gtcggccagc gtctggaaaa agatggcgtt
     1081 gcgctgacta tcgcggacat taaggccaaa ccaggaacac agtttgtcct gagccagcgt
     1141 accgaactgg aagcgattaa cacattgcag gaaaccttta ccgttagcga acgcagtaaa
     1201 gaaagcggga tgctggaact taccatgact ggtgatgatc cccagttgat tactcgtatt
     1261 ctgaacagca tcgctaacaa ctatttgcaa cagaatatcg ctcgccaggc ggcgcaggat
     1321 tcacaaagcc ttgaattctt acagcaccag ttacctgaag tgcgcagcga gctggaccag
     1381 gcggaagaaa aactcaacgt ttatcgccag cagcgcgatt cggttgacct taacctggaa
     1441 gccaaagccg ttcttgagca gattgtgaac gttgataatc aactcaatga gctgaccttc
     1501 cgcgaggcag agatctccca gctgtataag aaagatcacc caacttatcg tgcgctgctg
     1561 gaaaaacgcc agacgctgga gcaagaacgc aaacgcctga ataagcgggt atcggcaatg
     1621 ccttccaccc aacaggaagt gttgcgttta agtcgtgacg tagaagcggg ccgtgcggta
     1681 tatctgcaat tacttaaccg ccagcaggag ttgagtattt cgaaatccag tgccattggt
     1741 aacgtgcgga ttatcgaccc ggcagtcact cagccgcagc cagtgaaacc gaaaaaagcg
     1801 ttgaatgtgg tgcttggttt tattcttggc ctgtttattt cggtgagtgc cgtgctggcg
     1861 cgtgcgatgt tgcgtcgtgg tgtagaagcc ccggaacaac tggaagagca cggcatcagc
     1921 gtttattcca ctatcccaat gtccgagtgg ctggataaac gcacccgtct gcgtaagaaa
     1981 aatttatttt ctaatcagca gcgccatcgt actaaaaata tccccttcct ggcggtggat
     2041 aacccggcgg attctgctgt ggaagccgta cgtgcgctac gaaccagtct gcatttcgct
     2101 atgatggaga ctgagaataa cattctgatg atcaccggtg cgacgccaga cagtggtaaa
     2161 acgtttgtca gttcaactct ggcagcggtg atcgcccagt ccgatcaaaa agtgttattt
     2221 attgatgccg acttacgccg tggttattcg cataacctgt ttaccgtgag taatgaacat
     2281 ggcttgtcgg aatatctggc aggtaaagat gagctcaaca aagtgatcca gcattt
//
LOCUS       DAJTNM010000237         2222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTNM010000237 DAJTNM010000000
VERSION     DAJTNM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.241"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            34..>2222
                     /locus_tag="OO172_004140"
     rRNA            34..>2222
                     /locus_tag="OO172_004140"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          611 a          482 c          684 g          445 t
ORIGIN      
        1 tgatgagtcg aaagaaacat cttcgggttg tgaggttaag cgactaagcg tacacggtgg
       61 atgccctggc agtcagaggc gatgaaggac gtgctaatct gcgataagcg tcggtaaggt
      121 gatatgaacc gttataaccg gcgatttccg aatggggaaa cccagtgtga ttcgtcacac
      181 tatcattaac tgaatccata ggttaatgag gcgaaccggg ggaactgaaa catctaagta
      241 ccccgaggaa aagaaatcaa ccgagattcc cccagtagcg gcgagcgaac ggggagcagc
      301 ccagagcctg aatcagtgtg tgtgttagtg gaagcgtctg gaaaggcgtg cgatacaggg
      361 tgacagcccc gtacacaaaa atgcacatac tgtgagctcg atgagtaggg cgggacacgt
      421 ggtatcctgt ctgaatatgg ggggaccatc ctccaaggct aaatactcct gactgaccga
      481 tagtgaacca gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga gtgaaaaaga
      541 acctgaaacc gtgtacgtac aagcagtggg agcacgctta ggcgtgtgac tgcgtacctt
      601 ttgtataatg ggtcagcgac ttatattctg tagcaaggtt aaccgaatag gggagccgaa
      661 gggaaaccga gtcttaactg ggcgttaagt tgcagggtat agacccgaaa cccggtgatc
      721 tagccatggg caggttgaag gttgggtaac actaactgga ggaccgaacc gactaatgtt
      781 gaaaaattag cggatgactt gtggctgggg gtgaaaggcc aatcaaaccg ggagatagct
      841 ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt catctccggg ggtagagcac
      901 tgtttcggca agggggtcat cccgacttac caacccgatg caaactgcga ataccggaga
      961 atgttatcac gggagacaca cggcgggtgc taacgtccgt cgtgaagagg gaaacaaccc
     1021 agaccgccag ctaaggtccc aaagtcatgg ttaagtggga aacgatgtgg gaaggcccag
     1081 acagccagga tgttggctta gaagcagcca tcatttaaag aaagcgtaat agctcactgg
     1141 tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca tgcaccgaag ctgcggcagc
     1201 gacgcttatg cgttgttggg taggggagcg ttctgtaagc ctgtgaaggt gtactgtgag
     1261 gtatgctgga ggtatcagaa gtgcgaatgc tgacataagt aacgataaag cgggtgaaaa
     1321 gcccgctcgc cggaagacca agggttcctg tccaacgtta atcggggcag ggtgagtcga
     1381 cccctaaggc gaggccgaaa ggcgtagtcg atgggaaaca ggttaatatt cctgtacttg
     1441 gtgttactgc gaagggggga cggagaaggc tatgttggcc gggcgacggt tgtcccggtt
     1501 taagcgtgta ggctggtttt ccaggcaaat ccggaaaatc aaggccgagg cgtgatgacg
     1561 aggcactacg gtgctgaagc aacaaatgcc ctgcttccag gaaaagcctc taagcatcag
     1621 gtaacatcaa atcgtacccc aaaccgacac aggtggtcag gtagagaata ccaaggcgct
     1681 tgagagaact cgggtgaagg aactaggcaa aatggtgccg taacttcggg agaaggcacg
     1741 ctgatacgta ggtgaagcga cttgctcgtg gagctgaaat cagtcgaaga taccagctgg
     1801 ctgcaactgt ttattaaaaa cacagcactg tgcaaacacg aaagtggacg tatacggtgt
     1861 gacgcctgcc cggtgccgga aggttaattg atggggttag cgcaagcgaa gctcttgatc
     1921 gaagccccgg taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc
     1981 gggtaagttc cgacctgcac gaatggcgta atgatggcca ggctgtctcc acccgagact
     2041 cagtgaaatt gaactcgctg tgaagatgca gtgtacccgc ggcaagacgg aaagaccccg
     2101 tgaaccttta ctatagcttg acactgaaca ttgagccttg atgtgtagga taggtgggag
     2161 gctttgaagt gtggacgcca gtctgcatgg agccgacctt gaaataccac cctttaatgt
     2221 tt
//
LOCUS       DAJTNM010000238         2188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTNM010000238 DAJTNM010000000
VERSION     DAJTNM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.242"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(221..385)
                     /locus_tag="OO172_004141"
     CDS             complement(221..385)
                     /locus_tag="OO172_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514072.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1287..1571
                     /locus_tag="OO172_004142"
     CDS             1287..1571
                     /locus_tag="OO172_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5514073.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1559..2044
                     /locus_tag="OO172_004143"
     CDS             1559..2044
                     /locus_tag="OO172_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5514074.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          564 a          461 c          621 g          542 t
ORIGIN      
        1 ttcaaaatga tggacattat ttttgtggaa ccggaggtaa cagaccagac ggtttaaatg
       61 agccggttac tttaaacggg tgatggacag cacctcaaaa gttcagatgg attatatttg
      121 tctgcaatac cccgggtatt ttcgatttgc gaaatggatg gaacgacttg ctcaggggat
      181 agctgatgga gttactgaag taccgaagga ccactaaaaa tcacaggccg ggaatttcgg
      241 cgtttccggc gtaggtgtgc cacgaatgca tccagtggcg tgatgttgga tgcaatgctg
      301 tatgagagat gaggggcggc gtccagacgc agccttcaaa ccgccttgag ctgctgccgt
      361 taagagcggt ggtggtgagc gtcaaggtta aaaggcgcag aacatgtgtc aggtaggtgt
      421 tgcggagatg aacgaaagtg aactgtcgat gaagtgtcga aaggaacctc taggcgatgt
      481 cgaaaatgtg ctatctacgc agcgcatgac gagtccagca gatatctggt actggctggg
      541 cggcatccgg catataggca gcatgaacgc aacacaggct ctcgtacgga acgtgggaac
      601 ctgtcgtctc gatgctaagg gagcggcgcg gagcagaggc actgcaagcg cctgagtacc
      661 gatgcgagac acaggggcag atggcttcgt agtagtgagg aagtgcctgt aatgggcatg
      721 gagcgaaggg ggtcagtcgt tggggctggt ttatcggtca accggatagc cgggaggagc
      781 ccatgagtca gccaaagccg ttcgaggtct ctaagtatgc ggtttggaaa gcataccagc
      841 gggtcaaagc taatcgaggt gcggcaggtg tcgatgggca gtcagtcgag gcgtttgagg
      901 ttaccaatcg aagctatacc cggtaatggc gcaggttgac ctgcacttgg ccaagtggat
      961 tgcccgcaaa cacaagcgag cgcgaggtag tctggtgcgt gcattcgagt ggttactgcg
     1021 aattcgtcac gactgcccga cgctgtttgc acactggtgc ttagcttacg atacttgaat
     1081 ggttcaacga caagagccgt atgacgcgag agtgtcacgt acggttccgt gggagcgtag
     1141 gggggcggtg cccctgcgcc acccgatatc ccctttgagt gcggttacgt ccacagaggt
     1201 aaatgccgct gacgcggcat ctgtaatctg ttactttgtg tatatcattt gctatatatt
     1261 gtcaatatgc tattgttgta aagcaaatga tatacggagg ctttatgaaa tcaggtgttc
     1321 agctcaatct cagagcaaga gaatcacagc ggatactcat tgatgctgct gcagaaattc
     1381 ttcataagtc gcgtacagat ttcattctgg aaatggcctg taaagctgct gaagatgtta
     1441 ttctcgaccg gcgggtgttt aattttaatg acaggcaata tgaagagttc attgaaatgc
     1501 ttgatgctcc ggttgcagat gatcctgcca tcgaaaaatt gctggcaagg aaacctcaat
     1561 gggatgtgta actgcaccag aacctttatc ctcatttcat caggtcgctg aatttgtcag
     1621 cggtgaagct gtacttgatg actggttaaa gcagaagggg ctaaaaaatc aggcaatcgg
     1681 agccacccgg acttttgttg tatgcagaaa aggtacgcaa caaatagtcg gtttttactc
     1741 actggccact ggtagtatca atcatacaga agctacaggt aaccttcggc gcaatatgcc
     1801 agaccccatt cctgtaatta tacttgcacg cctggctgta gatgtgtcat ttcgaggaaa
     1861 agggcttggc gcggatttac ttcatgatgc agttcgtcgt tgttacaggg ttgccgaaaa
     1921 tatcggtgtg cgtgcaatta tggttcatgc gcttacggaa aacgcaaaac agttttatat
     1981 tcatcatggt tttaagccat caaaaactca ggtgcaaaca ttgtttctta aactccctca
     2041 atagggagta atgccgatca gttaaggatc agttgaccga tccagtggct gtgtaagaat
     2101 ccggaaacgc tcactcgttt ccggattttt ctgtgcacag taaaagacca cctcctgagg
     2161 aggtggcttt tcagaatgcc ccctataa
//
LOCUS       DAJTNM010000239         2187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTNM010000239 DAJTNM010000000
VERSION     DAJTNM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.243"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..270
                     /locus_tag="OO172_004144"
                     /pseudo
     CDS             1..270
                     /locus_tag="OO172_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            457..694
                     /locus_tag="OO172_004145"
                     /pseudo
     CDS             457..694
                     /locus_tag="OO172_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1115..1693
                     /locus_tag="OO172_004146"
     CDS             1115..1693
                     /locus_tag="OO172_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514075.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1802..2060)
                     /locus_tag="OO172_004147"
                     /pseudo
     CDS             complement(1802..2060)
                     /locus_tag="OO172_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2044..2187
                     /locus_tag="OO172_004148"
                     /pseudo
     CDS             2044..2187
                     /locus_tag="OO172_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          687 a          382 c          546 g          572 t
ORIGIN      
        1 ataaggggtt ccggatggtg atacggggtc ctgagtccgg tgaagcggcg attactggaa
       61 gatgtggtgt acgccataac ggagacagtg agaaaaacgg cgaggcaaat cacaaggaga
      121 gggatgtatc agcagtgact gaaggttgac ccgctgaagt gcgagctgcg cggaggtcag
      181 atgcggttta cggggctgaa gaggggttac cgtctggcag agcaggttat gatgcatgag
      241 ccgctggctc gaatacggtg gtgcggctga gagtcgcata ggggaagttg cgtccatgtt
      301 gaggggaacg gagcaaaaaa ccatcagaga tacattgaat caatcaatgt aagcaatatt
      361 aagtgtctat accgattact tgatgatcag gttgtgttga ataaacgcgc attcttcggt
      421 gcttttttaa tcttttgggg atttaaattc ctatcgatga actgtcgata tgaaaaagcg
      481 agcatcatac tgacgtcaaa caaaggggtc gcagactggg gagaaatgtt cggagatcac
      541 gtgctggcaa cagcgatcct agatcggctg ctacacgctg aatatcaaag gagagagtta
      601 ccggttaaaa gagaaacgta aagctggagt gctgaccaaa aacacaacgc caatcagtga
      661 tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag tatcttagca ggcatgaaaa
      721 tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg aaggagggag aatgccatct
      781 catcacttat atctggcgaa tgaaacacag atggtcggcc ttgcccccaa acacacggcg
      841 gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga aatatgatta aatcagtgtt
      901 atgttcgaac ctgacgtgtt aacagcagta aagtagtctc gcgaaggtgt tcaaacatta
      961 taatgaatgg catcctcata gtgcgcaggg ttatcgcttg ctacggaaat atctgaagcg
     1021 acgtaccagt aatggggaaa atgatagctg gtttctgaaa atatagagga aaatgtgata
     1081 tatatatcct gttaatattt aaggagaata taaaatgaaa gtctcgttta agtctttagg
     1141 ctatattttt catgatatat ataataaaaa acacactatc gatgagttca acgatgtggt
     1201 aagaaaagca gttttatcag gtaaaattaa cgagctgaat gcatgtcata aagtggcaat
     1261 ttttcttgcc gaaaaagaca atgagataac aaagaaagac aaagccaaga taatagatac
     1321 attaactgaa aactattcca ttgaatttca acaattaatg aatattagtg agcgcacgtt
     1381 aaattcatct ttatatatta ctcctggtga aagtggtttt gtcagttttg ttaatcgtga
     1441 aggaaaaata tgtcatactg catatgtaaa atcatcagat aatagcatgg catattacca
     1501 tgcaaatggt agcagcattg acaaatatat tacggatatg tgcggcctta tttgtatgag
     1561 gcatattgag tctactggca ttatttttta tatgcttgat gaaaaagttt tatctgccat
     1621 tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt tgttcagcaa agaacttata
     1681 taaatgtgtt taattttaga aaaactattt ggtaaaatcc aacaagagtc gagaggttcg
     1741 cgttgcttca gcagggcctc agcctgttca accagactca gttgcaggtc gtgacgcaga
     1801 tttagtggtg gtggttgatt gtcagatacc agccgatgat cttgtagtgc atatcatcag
     1861 actttgagaa gcatatggtt ttgcgggtaa gccgcttaag atgtgtccgc aggttcagat
     1921 tatgtcgctc tatccgctga gtgcatttct tgcttataac atgactggtt gaggacaata
     1981 aagctcggta taccggctca ggcatcggtc atgtagaagg caatgctaaa ctggctcagc
     2041 aaactaagag cagcgcgcat ccacttaccc aatgcggttg agaaaatagc cttcgatcgc
     2101 tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa cccgccagaa tgaacatccg
     2161 catcttccta ttgaaagtcg tcgtcag
//
LOCUS       DAJTNM010000240         2160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTNM010000240 DAJTNM010000000
VERSION     DAJTNM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.244"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..645)
                     /locus_tag="OO172_004149"
     CDS             complement(<1..645)
                     /locus_tag="OO172_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5514076.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSP"
     gene            993..1865
                     /gene="rluF"
                     /locus_tag="OO172_004150"
     CDS             993..1865
                     /gene="rluF"
                     /locus_tag="OO172_004150"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5514077.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(1998..>2160)
                     /gene="yjbD"
                     /locus_tag="OO172_004151"
     CDS             complement(1998..>2160)
                     /gene="yjbD"
                     /locus_tag="OO172_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5514078.1"
                     /translation="ETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWS
                     ANTSTRGRR"
BASE COUNT          525 a          545 c          546 g          544 t
ORIGIN      
        1 cggtgagcca attcccacca gggcgacatc cagattatcc cagtaggcag agatggtttt
       61 aaagtgctga gactgcatga tcccattacg aattaatggg ttatccagca gagccggaaa
      121 atccgcgaga tgcgattccc ctttcagctt cgctgccgcg ctgtaggtta atgtgtttac
      181 atgatagcgg ctttcgagtt taccggacgg tccgccaata attggtacgc agattaactg
      241 ccgcgattgc cccgcctgcg gcaagttttc aaccagtgcg ctcaccgcgc gaccccagga
      301 aaaaccgaca atatcgccag gctccagcaa gcgatccagc agttgcgcgc cgtgtaaccc
      361 catcatcgcc agttgagtct cttcatcctc atcatttccc gacaccacca caacgtcttt
      421 caggccaaac ttttgcttca gttgctgctc cagccagaga ttttcgttgt agtcatagtt
      481 gatggcgatg gtgaccattc cctgatcgcg gcctcgttta agcaagcggc tgatggtggt
      541 gcggtaaata cccagttcgc gcgcgatttg cgcctgcgtc atatcctgtt cgtaatagag
      601 ttgggcaatc ttcacaatca aacggatatc gtcactgttt tccattgccg cctcctggtt
      661 ctgcacattt gtgcaaatac gctttctcac cgcttgatct gatataaccc catcatgagg
      721 gcctttcaaa gcgatttttc actctttgca tggggagaaa tagcgctttc tcttgcacat
      781 ttgtttctct gcgtagtagt catcagcaca ggtgtgcagc gattcgtaat atgttgatta
      841 tccttatttt gccaggcaaa tttgcatcgt acttccggag acacttttgt gataaaaggc
      901 gtggcagatc acatttaatt tcgacgttgc cgcataaccc tgtttttccc cgctaagagg
      961 tacggtatat tccgcgtttt acaggagaat ttatgctgcc cgactcatca gtccgtttaa
     1021 ataaatacat cagcgaaagc ggaatttgct cacgccgcga agcggatcgc tatatcgagc
     1081 aaggcaatgt gttccttaat ggcaagcgag ccaccattgg cgatcaggtg aaacccggcg
     1141 acattgtgaa agtaaacggt cagttgattg agcctcggga agccgaagat ttggtactta
     1201 tcgccctgaa caagcccgtc ggtattgtca gcaccaccga agatggcgag cgcgataaca
     1261 ttgtcgattt cgttaaccac agcaaacgcg tgttcccgat tggccgcctg gataaagact
     1321 cccaggggct gattttcctc accaatcacg gcgatctggt gaataagatc ctccgtgcgg
     1381 gtaacgatca tgagaaagag tatctggtga cggtcgataa accgattacc gatgaattta
     1441 ttcgtggtat gggggcgggg gtgccgatcc tcgggacggt gacgaaaaag tgcaaagtca
     1501 aaaaagaagc gccgtttgtc ttccgcatta ccctagtgca ggggctgaac cgtcagatcc
     1561 ggcgcatgtg cgagcatttc ggctatgaag tgaaaaagct ggaacgtacg cgcattatga
     1621 acgtcagctt aagcggcatt ccgctggggg agtggcgcga tttaaccgac gatgagttaa
     1681 tcgacctctt taagctcatc gaaaattcct cttccgaggc aaaacctaaa gcgaaggcca
     1741 aaccgaaaac agcgggcatc aaacgtccag tcgttaagat ggaaaaaacg gcggaaaaag
     1801 gcggtcgccc ggcgtccaac ggtaagcgtt ttacctcgcc ggggcgtaaa aagaagggac
     1861 gctgattaac gcctcagact gatgaaaacg tccaaaaaga gctcggacag tcccctcgcc
     1921 ccttcgggga gagggttagg gtgaggggaa gattgtgtcc acacagacga gtgaaaatct
     1981 caggcgttga ttgttcatta gcgcctgccg cgcgtcgacg tatttgctga ccagctaaat
     2041 gacgcttcag gatccggttt ataagcctgc tttttcttca attggcgggc ttttttcgct
     2101 tcagcctcac gctcgaccat taatttatcg atgtactctt tcttaatgct gttggtttcg
//
LOCUS       DAJTNM010000241         2160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTNM010000241 DAJTNM010000000
VERSION     DAJTNM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.245"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..2067
                     /locus_tag="OO172_004152"
     CDS             <1..2067
                     /locus_tag="OO172_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514079.1"
                     /translation="LGVPGMPATTKGIRQALQRFSRDLGDVSRRREGTKAIEYHIDCL
                     PEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGSPQLMEERLNA
                     LTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPERLQQAAVNAN
                     AKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEISWLPEFLAQYR
                     QVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEVVKQKGRVTGS
                     EYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTFVIDGGTRFVV
                     GWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVVGILPRLGIKH
                     PTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRDLRSAFNALQQ
                     DKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRHYSPTEFRKKR
                     LAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDEHGRKVLISYD
                     IHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRKRAEEKIRDAE
                     DEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRLGMQKLFAMQE
                     EDDPLA"
BASE COUNT          594 a          510 c          610 g          446 t
ORIGIN      
        1 ctcggcgtac caggaatgcc agccaccaca aaaggcatcc gacaggcgtt acagcgtttt
       61 tctcgtgatc ttggtgacgt tagtcgccgt cgggaaggca caaaagccat tgagtaccac
      121 attgattgtc tgcctgaaat aacacgaaag gcactgcgtg aacgctatgt ggaacagctg
      181 gtggctacag agaacaatgt ttctgaagtg aaagctgtta ccagaaaaac acgcaatcct
      241 gacgctgtcc aggcaatcga agcatatcgc ggttctccac aactgatgga agaacgcctg
      301 aatgcgctga ccgaaaacca gcgctgggta tctgaagcaa gagctgcgct ggtggtggaa
      361 gtgctgaagc tggaaagcgc cggtaacctc gggcgactga aagccattaa ctttcttgtt
      421 gaaaaagccc gtaaaggtga gctgccggag cgcctgcaac aggccgcagt taacgccaat
      481 gcaaaacgtg gcgctaatcg tactatcagc cgcgatccac tttaccagtg ggttctgaaa
      541 tacaaccagt cgcagaacgc cgccgaacgg ctgctgttac tggcaccggg aaaacgcgac
      601 gaaataaaac cggaagaaat cagctggctg cctgaatttc tggcgcagta tcgccaggtg
      661 aacggcagac ccatgtcaga agcctacgag gattttgtcg cagaatggca gcgtcgccat
      721 gcggacgaac cgtacatgct ggaggtgatg ccttcctatg atgttgtccg gtatgccatg
      781 aaaaaactgc cggaagtggt gaaacaaaaa gggcgcgtta ccggcagtga ataccgtcag
      841 cttgaaggat tcacccgccg cgactggaca gctatgccgg tgaattatgt ctggattggt
      901 gacggtcacg gtatgaagct gaaatgtgcg catccgatcc acgggcgtcc attctcaccg
      961 gaagtcacgt ttgtgatcga tggtggaaca cggtttgttg ttggctggag tcttgacctt
     1021 gccgaaaacg tgttcgccgt tgccggtgcg atacagcacg gcattcgcaa ccacggaaaa
     1081 ccgttcctgt attactcgga taacggttcc ggtgaaacgg ctgacatgct ggacaaagag
     1141 gtcgtgggta ttctgccacg cctggggatt aaacacccta ccggtattgc cggtaatcca
     1201 cagggacgcg gcattattga acgcctgaac cggacactgc cgatgcgtat agccaggagg
     1261 tatcgcacct attttggcaa gggtgctgac cgggaatcgt tgcgtgtact taaccgtgat
     1321 ctgcgttcag cgtttaacgc cctgcaacag gacaagcctc tgaacgaccg ccagaaagcg
     1381 gcaatgcgcg agctgccatc gtgggcggag cttattgagg ctattcgtga aggcgtggaa
     1441 tggtacaaca accgcccgca ttctgagctg ccgatgaaac cgaacggacg gcattacagc
     1501 ccgacagagt tcagaaaaaa acgcctggca gaagaagaca ccgaaattga gtggctgagt
     1561 gatcttgagc tgcgcgacat gttccgccca atggtggaac gaccggtaag acgctgcgaa
     1621 attcagtggc tgaacaatat ttactacgcg ccggagttgc gtgacgaaca cggtcgcaaa
     1681 gtgcttatca gttatgacat tcatgacgcc gagcgaatta cggtacgtcg caaggacggc
     1741 agctttatct gtgaggcgat atggaacggc aataaacgcg cagcctttgc tgtcagtgct
     1801 gaatatcaca aacaacagca gcgtattaaa ggaatgcgca aacgcgctga ggaaaaaatt
     1861 cgtgatgctg aagatgaagg tattcagatt ctggaacaca aacaggctga accctggctg
     1921 agtaatgttt atcgcccggt tggtaatgtg gtggcagttc agcaaccgga atatgaagaa
     1981 gagcatgacg aagaatttga acgcgatttc cggctgggga tgcaaaaact ttttgccatg
     2041 caggaagaag atgatccgct ggcctgaata aaaaaacagt ccgcgggaac ggactgttaa
     2101 ataaaggaaa caaagctgat ttgaaataac cgaggaaata ctatgactga tattgatgat
//
LOCUS       DAJTNM010000242         2154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTNM010000242 DAJTNM010000000
VERSION     DAJTNM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.246"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..169
                     /locus_tag="OO172_004153"
     CDS             <1..169
                     /locus_tag="OO172_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5514080.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            154..792
                     /locus_tag="OO172_004154"
     CDS             154..792
                     /locus_tag="OO172_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5514081.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1020..1421
                     /locus_tag="OO172_004155"
     CDS             1020..1421
                     /locus_tag="OO172_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514082.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEISLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSSGGGR"
     gene            1432..1906
                     /locus_tag="OO172_004156"
                     /pseudo
     CDS             1432..1906
                     /locus_tag="OO172_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          644 a          398 c          505 g          607 t
ORIGIN      
        1 gtccgctatt gccagtacac ctcacccgtc ctggcgacgt atctgtaaga cactcataaa
       61 gaatgatttt tggtgccgga ctctctcctt cagtccgaac aaaccccggc actatgaacg
      121 ttacctgcag cgtatgaagg aaaggaggaa ggaatggggg attctgtgac accggagata
      181 cttggcaaca tgattcgtca gtatttcagt caggtacgaa ctgaaaaaga gagcatacag
      241 gctctgaatc atctgcgcag agtgcttcat gaggtcagtc cttttgcaca ggagcctgtg
      301 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa ccctaatgtc
      361 atggcactag gtgagaaaaa actactgaaa cagtctctgg agaaagatgg tttcacacaa
      421 ccggtggtgg tgtcagagga gaagaatcat tatctggtgg tggatggttt tcatcgtcag
      481 ttactggggc gggaagcaga tacaggaaaa cgcctgaggg gctggttgcc tgtagcctgt
      541 attaatccgg agagaaaggg acaggccgca cgtattgccg cgaccattcg acataaccgg
      601 gccaggggga aacaccagat cactttaatg tctgatattg tcagggatct ttcacgtctg
      661 ggctggacga atgaacgtat tggtacagag ctgggaatgg accaggatga agtgctgagg
      721 ctgaaacaga ttagcgggct gacagagttg tttcaggaag aggatttcag ctcggcttgg
      781 acggttcgtt gatgttgtta aatatgggcg gtatttctga aagttgatac tttgggtaga
      841 aaaaatgata ccgaaaacta tggaggatgt gcttgtcagg tgactggaca ccataggtct
      901 taggctttga taacgggata aaatgagcgg cttttctcat accatcaggt gtgatgatgg
      961 cgcagattta caaaatctga ttgattctaa agatgtataa gtaaaaatca agctttgata
     1021 tgaaaaaaca gatatttatt aataataaac ctcctgtagt cccttactca gggacgcacg
     1081 cgaaaatatt taaatacatt gaaatctctc tgccgttttt ctactttatc tatacatcag
     1141 gcgaaccatt ccacatatct gtccaaaata ctgttattta tgtatcaaaa tacaatggta
     1201 tatttattaa taaattagtt cctttttctc ttttatttga cagggatata tctgttcttc
     1261 aaagaagaga tatttgtgtt gtgcgattta cgtcagagga aataagtgaa cataacgttt
     1321 tatttgatca tgacattgaa cgattaaaaa aaatttccaa agctcagctt atatctcccg
     1381 attatgtttt gattgatttt tctagtgggg gggggagatg aaccctatgc aatgtcctgg
     1441 ttaatatctc agtgtgctca tcagtgcact gataataaaa agacggagac agatgccatt
     1501 tacgataaag ttcggagttc ttatttgctt tcatgtattc tgaaaaaaaa caaaaatgtt
     1561 gggcttattc ttcatgcacc atcatttgtt tctgtgtctg aaaaaaatcg ccagaattgt
     1621 gatggcaaat tactcccgta actggagtaa ttccgagttg gccagtgcgg ttttaatgag
     1681 tgaatcttct ttaaaaagaa gaatgtacaa agaggtcggt tctataagta catttgtaca
     1741 taaaataaag cttacagaag ctatcaggaa attacgaagg acaaatactc ccatttctgt
     1801 catatcatct gagttaggtt acagttcacc atcgtatttt tctaaagttt tctttaaata
     1861 tttaaagaca taccctcaaa acataaggaa gaaaaatggt cggtaatgtc accaattagg
     1921 aggacttgca gagccgcttt tttacgatac tgagatcaaa cagacataat ctatgcggag
     1981 atttacagca gggcatggtt cacaaatctg acagcgatga attatcggca ctgcgggcag
     2041 aaaatgctcg aatcatcaag cccctgttac ctcctgaacc cgccacacca cgggccggac
     2101 gcccatgggc tgagcaccgt aaaatcatca atggcatgtt ctgggtgtta tgtt
//
LOCUS       DAJTNM010000243         2104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTNM010000243 DAJTNM010000000
VERSION     DAJTNM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.247"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..278
                     /locus_tag="OO172_004157"
                     /pseudo
     CDS             1..278
                     /locus_tag="OO172_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            447..1811
                     /locus_tag="OO172_004158"
     CDS             447..1811
                     /locus_tag="OO172_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5514083.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            1861..2070
                     /locus_tag="OO172_004159"
                     /pseudo
     CDS             1861..2070
                     /locus_tag="OO172_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
BASE COUNT          556 a          399 c          475 g          674 t
ORIGIN      
        1 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
       61 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      121 aggactggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      181 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      241 caagaataaa tagtagtaac tactatagtt gcaaataatg ttaaaaacat ttctatgatc
      301 atctttacat tttttatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      361 tgataatgca atactttgag taattatata atgataagta aacaggtttg cgacaattgg
      421 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      481 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      541 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      601 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      661 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
      721 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
      781 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
      841 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
      901 cttgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
      961 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga gcatgcccgg gccgcattta
     1021 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1081 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1141 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1201 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1261 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1321 aggatatggg aaaactggca tcaatggggg ggttttctgt ttttgaaatc ggtggtgtgg
     1381 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1441 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1501 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1561 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1621 gagccgcatc agggctgtca ggcacatttt cctatatctt cggctcaatt gttgcgacac
     1681 tgggaatggg acttgttgtc gattatctgg gatggggggc cacatttatc gtactgatac
     1741 tgtctgctgt ttttgccatc attttcacac tgatgagtcg tgaacgctca ctggagttcg
     1801 aaaaagagta attttctcag gaaaaacata aaattactga ctaaatattt attttttgaa
     1861 tcgcagggca ttccggataa ggttgatatc gtgtcacggc tgaagaccta tggatacagt
     1921 atttctggtg tggagacgga tgacggatat aaggcgcttg tccgcgcttt tcagcttcat
     1981 ttccggcaga aaaattatga cggcataatg gatgctgaaa ccgctgccat cctgtatgca
     2041 ttgctggaga agtatttccc gggtaaataa ggagtgagtc gtcagagctg cgatatatcc
     2101 cctt
//
LOCUS       DAJTNM010000244         2055 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTNM010000244 DAJTNM010000000
VERSION     DAJTNM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.248"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(55..1923)
                     /gene="kup"
                     /locus_tag="OO172_004160"
     CDS             complement(55..1923)
                     /gene="kup"
                     /locus_tag="OO172_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5514084.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
BASE COUNT          599 a          554 c          490 g          412 t
ORIGIN      
        1 tgattaacca tgaaaacaaa aacgccccct tatgaagaaa ggaggcgtct ggcgttagat
       61 ttcgacctga gtacccagtt cgataaccct gtttggcggg atttcaaatt gatctggtgc
      121 acgcagcgca ttacgttgca gcagcaagta cagcttgccg cgcagacgca aataccacgg
      181 gcgtttgccg aggatcaacg actcatgcga cataaagaag gaggtttcca tcatccggca
      241 acttaatcct tccagaccgc agcggtggaa aacttcttcc acgtttggcg tttctcgcca
      301 accataactt gccaccacgc gccagaaagt gggcgacagt tgttcaatct gtacccgacg
      361 gacgttatgg acatatggcg cgtcttcggt gcgcagagtt aacaaaatca cccgctcatg
      421 caataccttg ttatgtttaa ggttatgcat cagcgcaaag ggaatgacgt tgattgcacg
      481 cgacatatac accgcggtcc cgggcacgcg aacaggcggt gatttctcca gcgaagcaat
      541 catcgcttcc agagagttac catgttcatg catccgccgc agcaagcgga aacgctcgct
      601 cttccaggtg gtcatcacga taaacatcac agtaccgagg ctcaatggca accagccgcc
      661 ggagagcagt ttatcgaggt tagcggtgaa caatggaata tcgacacaaa ggaaagcaat
      721 caggatcagc gcaacaaaat acttattcca gtgccagttc tgacgtgcca cggtagtcga
      781 gagaatagac gtcagtacca tggttccggt caccgcaatc ccgtacgccg ccgccaggtt
      841 gctggagtgc tcaaagctga caatcacaat cacgaccgcg acatagagca tccagttcac
      901 aaacggaata tagatttgcc ctgactccat ttcggaggtg tgaataatgc gcatcggcga
      961 caaatatccc agacgtaccg cctgacgcgt caatgagaag acgccagaga taaccgcctg
     1021 cgaggcaatt accgtcgcca gtgcggcgat gatcagcagt gggatcagcg cccagtccgg
     1081 tgccaacagg aagaacgggt tcttaatcgc ttccgggttc tttaacaaca acgctccctg
     1141 accgaagtaa ttaagggtca aggaaggtaa tacgacggtg aaccacgcca ggcgaatagg
     1201 gaacttacca aagtgcccca tatcagcata cagcgcctcg acccccgtaa tcgacagcac
     1261 cactgcccct aatgcaataa aagaaaccgt tttgtattca aggaagaaat gcaccgccca
     1321 catcggattc agcgcatgca gcacttccgg gttagcaata atgctacgta accccagtcc
     1381 tgccagaatc aaaaaccagg tcagcatgat cggcgcaaac agcttaccga ccatagccgt
     1441 gccatgcttt tgaatcataa ataataaagt gagaacgata attgagaggg gaactatcca
     1501 ggtatccagc tgcggggcga cgatttccag accttcaatg gctgacatca ccgaaatagc
     1561 gggtgttatg acgacttcac catagaaaaa gctgccgccg attagcccca taatcaccag
     1621 cattgatgtg gttcgcgccg acgtattacg cccggcaagc gacatcaacg tcaggatccc
     1681 cccctcaccg gcgttatctg cccgcatcac gaaggtgaga tatttaatgg aaaccacaaa
     1741 gattagcagc cagaagatca gcgataaaaa gccaaacacg gcatcgcgtt caacgccaaa
     1801 accaaactgg ccggacaaac attcacgaag tgtatataac gggctggtac caatatcgcc
     1861 gtagacaact ccaatcgccg cgagggtaat cgcgggcaat gattgcttat tatcagtgct
     1921 catagactag tctttcgttg aaatatgaaa tgtgtgctta gtcccttggc ccacaaaaaa
     1981 gcgcacagta tgcacgatta acggcaaaat cgtactccta aatgcggcca cattaacctg
     2041 gctcaaagaa aaagc
//
LOCUS       DAJTNM010000245         2023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTNM010000245 DAJTNM010000000
VERSION     DAJTNM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.249"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            414..1922
                     /gene="ltrA"
                     /locus_tag="OO172_004161"
     CDS             414..1922
                     /gene="ltrA"
                     /locus_tag="OO172_004161"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5514085.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          507 a          503 c          592 g          421 t
ORIGIN      
        1 cctctgtggg ttgaaaacgt caccacggcc tacgtgatct gacaggccgt gcaggaggaa
       61 ctggcagtga tacgtaagca ctgccggtcg aaggtgtttt gacatgtatg cgaaacaccg
      121 gggcagcagc gtctatcacg cttgcgttgc tgacttctgc caacttgcgg caagcaagga
      181 taaagagtgc gtcgggcagc ctcctcagta tgcctgagtc caggcaggta aaccggggaa
      241 ggtcagcgac ggatgttaag ggggcatggc tccgatgacg cgctggctgg cggagcttcc
      301 gtagtagtcc gcgatgggga aagcccatta catggcgaag ggaagcagtt tgaatgtgtt
      361 tgcgacgtga attaactgac ctaacgaggt gaagaccttt gataatcagc gaaatgcaac
      421 gcaagcttgc cacatgggca gtcaccgatc cgtccctacg gattcaacgg ctgctgcgtc
      481 tgataacaca accagaatgg ctggctgaag cggcgcggat cacgctttca tcaaaggggg
      541 cccatacccc cggcgttgat ggcgtgaaca aaacaatgct acaggccaga ctggctgttg
      601 agctgcaaat cctcagggat gaattactct caggccacta ccagcccttg cccgccagac
      661 gggtttacat ccctaaaagc aacggcaaac tgcgaccact gggtatcccc gcgttgcggg
      721 atcgtattgt tcagcgggcc atgctgatgg cgatggagcc gatatgggag agtgattttc
      781 atacgctctc atatggcttc cggcctgagc gcagtgtcca ccacgctatc cgcacggtga
      841 aattacagct cacagactgt ggtgaaaccc ggggacgctg ggtgattgaa ggcgacctgt
      901 ccagttactt cgacaccgta catcatcgac tgctgatgaa ggctgtacgc cgcaggatca
      961 gtgacgcacg tttcatgact ctgctgtgga aaaccatcaa ggcgggacat atcgatgtcg
     1021 gtctctttcg ggcggccagt gaaggtgtgc cacagggcgg tgttatatcg ccgctattat
     1081 cgaacatcat gctgaatgag ttcgatcaat acctgcatga gcgctacctg agcgggaaag
     1141 ccagaaaaga tcggtggtac tggaataaca gtatccaacg gggccgaagt acggcggtca
     1201 gagaaaactg gcagtggaaa cccgcggtgg cgtactgccg ctatgccgat gattttgtcc
     1261 tcatcgtcaa aggcaccaaa gcacaggcgg aagccatcag ggaggagtgt cggggtgtgc
     1321 tcgaaggcag tctgaaactc aggctgaaca tggataagac taaaatcacc catgttaatg
     1381 acggctttat ctttctgggg cacaggatca ttcgcaaacg cagtcgttat ggcgagatgc
     1441 gagtggtctc aacgatcccg caggagaaag ccagaaactt cgccgcatcg ctgacagcac
     1501 tgttatcagg caactacagt gaaagcaaag tcgatatggc tgaacaactc aaccgaaaac
     1561 tgaaaggctg ggccatgttc tatcagttcg ttgattttaa ggccaaagtc ttcagttata
     1621 tcgaccgtgt cgtgttctgg aagctggctc actggctggc ccgcaaatac cgtacaggta
     1681 tcgcttccct gatgaggtgg tggtgtaaat caccgaaacc gggtcagagc aaaacgtggg
     1741 ttttatttgg taaaaccaat cacggcaagc tcagcggcga aatactgtac cggttggtgg
     1801 ggcaaggcaa gaagctgttc cgctggcggc tacccgaagg taatccctat ctgaggacgg
     1861 agaccagaaa cacgtataca tcgcgcttta cagaagtggc aatggcgttc gccagcattt
     1921 aaatggagag ccggatgcgc tgaaaggtgc acgtccggtt cggggaggag aggcagggaa
     1981 atagtccgac tacgccctgc ctcttactct actcagcaca tgg
//
LOCUS       DAJTNM010000246         2015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTNM010000246 DAJTNM010000000
VERSION     DAJTNM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.250"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..606
                     /locus_tag="OO172_004162"
     CDS             <1..606
                     /locus_tag="OO172_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5514086.1"
                     /translation="AKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAM
                     VFNGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            638..1246
                     /locus_tag="OO172_004163"
     CDS             638..1246
                     /locus_tag="OO172_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514087.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKSVLAVNSEQFRGLTVERLAKYIEMA
                     KHIDHEISDNGYRPSEGYLPAAERRSLMLAFGVSGVSMWPSA"
BASE COUNT          566 a          438 c          498 g          513 t
ORIGIN      
        1 gctaaagcgt actcgcagaa gacctcaggt aagccatacc attattacgt caccgctctg
       61 gctgcgatta caggtgcgag acttaacgag gtggcccaac ttcaggttaa agatgtcagg
      121 accactgagg ctgggaccgt ttttatccat atcaacgaag acgatagcag cctgccgggt
      181 aagagtatca agaacgccca cagtgaccgc tgtgtcccgt tggtagatgg tgcttatggt
      241 tttgtattgg ctgactttat gtcactcgtg gaagaccgta gaaagactga gggtgataat
      301 gctatggtgt tcaatggcct caagctaatg aagaacggct acggtgagca ggttagcaag
      361 tggtttaacc gaacgttgct gcccaaagtc ctcgctgacc gtagcggctt agcttttcac
      421 tcgtttagac atacagttgc gacccagtta aaacaacatg gggtcgagtt agcgtatgct
      481 caggcgatta tgggtcatag ctcagggtct ataacttacg accgttacgc gaaagaggtc
      541 gaggtggaaa cgctgaaaga aaaattagcg gagtcattgt cagtaaaaaa aatagacgga
      601 aagtaatcgc atagggatag cggaatgtga ggacgtaatg gtcaaagaat ttttagttag
      661 caaagaaaat aagccttcat atgaatgtat gaatactgct gaaacttgtt tggggctggc
      721 tggaatggct tcggaagatt ggcaacgtga gcatcattgc cgcacaggta taatatttac
      781 tgcttttgct attgaggcaa tgttcatctt ctatagaaaa caggttgacc ccggctacga
      841 taagacgcag aaagagtgca gaaagacaat gcacaagaat acgttaaaat tgtgcggtat
      901 aaacaattat atggggacaa aaccttacca aattatcaaa gagtgtcttg aggtaagaga
      961 cgctattgct cacggcgatt cgtatacttc gagctttaat ttctcggcgg atcatttgga
     1021 taaccaagac gatattgcaa aaagtgtcct ggcggtaaac tctgaacaat ttcggggatt
     1081 aactgttgag cgccttgcaa aatacattga aatggcaaag catattgacc atgagatttc
     1141 tgataatggt tacaggcctt ctgaaggtta ccttccagca gccgagcgca gaagtctgat
     1201 gttagctttt ggcgtcagtg gtgtgtcaat gtggcccagt gcttgagcga taatacacct
     1261 cactatagag gaatggaccc ttaactatca ctatagggga gagaccctaa gtccccacct
     1321 taatagcagt cacgacccat acacacattc attaagtctc aggcttccgg tagcggaccg
     1381 attggaaacc atccgcagac gcctagtgtt aaatcacctt ggcctgagac cattcctttt
     1441 aacgtaagct atcttccctt ttgacaggcg gggagcagta gaccacatcc gactttaagt
     1501 tggctctgtg ataatcgctg cgcggataac tgaagaacca agacaccatt actgaggtct
     1561 gccatccatt tgtggacagg cggaaactca aagaggttag atcagtggtg tctgaggttg
     1621 gagctttgag tcttgaccag caggctggag gagaccatga gtttatcttg ttatggattg
     1681 tgttatcaca attccaataa gaagaacttt aagtccagcc tccactcccg ccaacccgaa
     1741 gtaaaattat ggtcgctatg atgtggtctc catgcttcgg gtcttactgg aagtactgaa
     1801 cactttcgcg tcaagttctc cgaagttcat tcctgtttga ctaaactatc actataggga
     1861 ccatatctcg cctctgacct cctgagagac ggcaagcgag aaaccgtggt ccacgctagc
     1921 aagctagcca ccagcctaag gcaagacagc ctctggtcag cctgtgtgtt ctttggtctc
     1981 gtgacttaag tctatcacca cacacgctcg gacat
//
LOCUS       DAJTNM010000247         1914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTNM010000247 DAJTNM010000000
VERSION     DAJTNM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.252"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(84..596)
                     /locus_tag="OO172_004164"
     CDS             complement(84..596)
                     /locus_tag="OO172_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5514088.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            791..1555
                     /locus_tag="OO172_004165"
                     /pseudo
     CDS             791..1555
                     /locus_tag="OO172_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1506..>1914
                     /locus_tag="OO172_004166"
     CDS             1506..>1914
                     /locus_tag="OO172_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCR5514089.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQW"
BASE COUNT          562 a          412 c          477 g          463 t
ORIGIN      
        1 attttactca ttatccttca aatcagattc tgttgtcaga ggaatggggg aagctggatt
       61 cgcaagagct ttttatgggc agtttagcta aaaaaaggca acacgaagct aaaactacca
      121 acataaagtg ctgaccagct agcagctgaa atcagactca ctgtatacac tttcactgga
      181 tgaagttgca acattcctgc caccaaagga gcaatgtaac gcagcacggc gataaagcgc
      241 gaagtgaaaa gtattgacac agagttattt tgcaactgaa ggcgaacccg ttctattgta
      301 ttggaacggc tcgatattat acatgcaagt tgagggatat gcccaattat tataccaaga
      361 tggtaattaa cgatagtacc gcaccatgcg ccactcatga ccgtgattcc agcttcccat
      421 ggtgaaagag tcgtcttgct gactgtaatt acagtggcca tcataattga tgctggtggc
      481 aatatcgccg aaatgagtaa agtggatttt gtgaacgcta ttataaaaag aagcccccat
      541 aggcgatatg gatgaagtac aaaatagttc atcaaagcat ttatccactc catcaatgtc
      601 tttccttctg tgaatttttc tttgctattt aaccgtgata cgacttaatt taggcttaaa
      661 caccttacat ttacaaggta ctcctgtggg gggccctgcc acggagcgtc gcaggagtga
      721 gtgatgagga agaattaaag caacctattg tgacaggcaa cgataacagt aaatcacagg
      781 aagcataatc atggcaaaac cggactggga ggccatcgaa tcggcatacc gggccggagt
      841 ccttagcctc cgtgatatag gcgagaaata cggcgttaca gaaggggcta tcaggaagag
      901 ggccaaaaag cttggttggg cacgcagtgg cggtacgcag gtttgcaaaa atggtacgca
      961 aaaaaggaaa gtgcgtacca gcagaaagcc tgccattact ggccttacac aaaaaagtac
     1021 gcaactaaaa acagaatcta caccggatac gaaaccgata cgcggaatgc gtaccgatcc
     1081 cccgactaac ccattccaac ccggtaacca gcaggcatta aaacacggtg gttatgcccg
     1141 tcgccttctg ctcaaagatg aggtgataga ggacgctaaa gcgttgacgc tcgaggacga
     1201 attatttcgc cttcgtgcta acaaccttgt cgctgcagag aatattggtc ggtggttggt
     1261 gtcgctggaa gatgctaatg gggactagga aaggaagatg ctgattgaaa atatcagcgc
     1321 cgccgagaaa gcaatgatgc gcaatacagt tcgtattgag tccatcgttg gcacgcttgc
     1381 gacggtagga aaaatatttg ctgatacagc ctaccgcaag gccgccactg ataaggtgtc
     1441 tctggaggct gatcgtcttc gccgtgatgc aggtattgat gatggcaatg gagagcgtga
     1501 cctcaatgac ttctactctg acatccaaac cgacgctgaa tccggcttta cgtagtttct
     1561 ggactatgcg ggcacgtaac aaagtgcttt atggtggtcg gtcatcgtca aaatcatggg
     1621 atgccgctgg cattgccata tttctgtcga ataaatacac cctgcgtttt tgttgtgccc
     1681 gtcagatcca gaataaaatc gaagagtcgg tgtataccct gctcaaaatt cagatagaca
     1741 ggtttggtct gcggcaccgt ttccgtattc tgaacaacaa aatcattaac cgggttactg
     1801 gctcggaatt tgttttttat ggattatggc gcaacatcga agaaattaag tcactggagg
     1861 ggatcgatgt gttgtggctg gaagaagccc acgcactgac ggaataccag tgga
//
LOCUS       DAJTNM010000248         1877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTNM010000248 DAJTNM010000000
VERSION     DAJTNM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.253"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..557)
                     /locus_tag="OO172_004167"
     CDS             complement(<1..557)
                     /locus_tag="OO172_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5514090.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
     gene            complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="OO172_004168"
     CDS             complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="OO172_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR5514091.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1321..1451)
                     /locus_tag="OO172_004169"
                     /pseudo
     CDS             complement(1321..1451)
                     /locus_tag="OO172_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1449..1544)
                     /locus_tag="OO172_004170"
                     /pseudo
     CDS             complement(1449..1544)
                     /locus_tag="OO172_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          536 a          319 c          395 g          627 t
ORIGIN      
        1 atgctgtcta atctgtttcc agaaactacc agtaatttta gttccggtgg caatagtggc
       61 agaatatgca atcggttatt atgaacccgt aaatgagtta gattttcagg aagaaatggt
      121 aattctttta tcttgttatc acccgcatta agctcttcta acgtctcggg taaggcaggt
      181 aagctttcca gttgattaaa ttgagcatgt attaccttaa gcattggtgg taaatcaggg
      241 agatgtgtta ataggttttt tcttatttcc agcgttgtta tctgaggcgg taaatctggt
      301 aatgttttta aatctaactc agataaattt aattctgcct cttgattttg taaacatatt
      361 ttcagcctat tgaaggcgat attgcgttgc tcgcctgggg taccgttctt ttcccattcg
      421 ctccaggctt ttaaataaaa ttcattacaa gatggagtca gttccatagg taaagataat
      481 ggagttaacg agtatatatt gtttatttga tgcataatgc cagaaccgat tgcctgtata
      541 ttggttgatt taatcattat aaccagaatt cttatcaacg ataagaattc tggttaaata
      601 gaatatggct tattaatttt aaaacagttt gctttgtacc ctttctccgg gtattgcttt
      661 ccccctaagc cgtctatggg aaggagtctg ctgttattcc tgagaatggt aattcagcca
      721 taaaaaggta tttaaggtca ttgcgtctct ccctatactc gaataatggt gaagaaagca
      781 tgatgaatat atcacgataa cgttcagtac gtatcgagga tagatggagt tactcctctc
      841 cgaaatagaa ttaccgctct atttttttct gttttcagaa gttattagta acaaaccctg
      901 atatattttt ttgaaaatgg agcttaaacg agtctgaaat taacttcaga aatcagaata
      961 ccgttggctt tgtttttcat caattccggc ttcatttatc tttgtcaacc attgcatttc
     1021 tgtagcagtg aatttcaacg ttttcgtccg gctggcagca ggtatttgta attcgtatat
     1081 agctgctttt ttattgaaaa cagtatatcc atctgtagta acagtagaag ataataatac
     1141 aggatttgat acttcaagga tgatattttc ttgtttttgt ggaaggcaag ggaatatagt
     1201 ttgtgtaagc atattacttc cttatgttat attttggtaa aaaatttatt tttttacatt
     1261 atagtcggct aaatgaagtt tactgtaaaa aaacgatact ttttcgggtg gcgcttccaa
     1321 tcagtagaat atgtgttttt caatgaagga gccgatgact tttcgtggat ttccacggaa
     1381 cgcgagaagc agattgtttt acgtgccagc cgcttgatgc gggtgcggag ggtcaggttg
     1441 ttacgttcaa gatatagcat gtgctggatg aagatccctt ttctggtcac ctgtttatct
     1501 ttcgtggccg acggggtgac actgttaaaa tgtaatgaac gcatcccaca caattaaaca
     1561 cggagtagta tgatggttaa aagggtgaaa ctggcaggtg tggagcctcg cagctttccg
     1621 gcatcatact gcccaatacg ggatgcataa agcgtcctca gactggagac tccacaaatg
     1681 aaatatacac cggttggcgt tgatatggca aaacatgtca ttcagattca cttcatcaat
     1741 gagcacatgt cagcgccagt gatataagac ggtaatgggg atgcggacga tttcctggac
     1801 tctcagaaga tgaaacatgt ttactgaaga acaaaaaatt cgcgctattg agctttattt
     1861 caaatatggt aagaagc
//
LOCUS       DAJTNM010000249         1859 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTNM010000249 DAJTNM010000000
VERSION     DAJTNM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.254"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..559
                     /locus_tag="OO172_004171"
     CDS             <1..559
                     /locus_tag="OO172_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5514092.1"
                     /translation="LGLARSSYFYHRAALKAGDKYATIRKMLTDITEFQLPAGKVWLS
                     PVVDCFDGKVVSWSLSTRPDAELVNTMLDSAVETLNAGERPVIHSDRGGHYRWPGWLE
                     RVNAAGLIRSMSRKGCSPDNAACEGFFGRLKTEMYYGRKWSGITPEKFMQQVDAYIRW
                     YNERRIKLSLGAVSPKMYRQQCGLE"
     gene            674..1015
                     /locus_tag="OO172_004172"
                     /pseudo
     CDS             674..1015
                     /locus_tag="OO172_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            998..1835
                     /locus_tag="OO172_004173"
                     /pseudo
     CDS             998..1835
                     /locus_tag="OO172_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          455 a          454 c          571 g          379 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatcacggag ttccagctcc ctgcaggtaa
      121 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      181 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      241 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      301 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      361 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      421 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      481 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      541 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      601 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      661 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      721 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
      781 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
      841 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
      901 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
      961 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1021 ggccgaacgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1081 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1141 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1201 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1261 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1321 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1381 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1441 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1501 aatgcagacc cgggataacc ttgagcgcat ggtggttatc caggcgttta tagcggtacg
     1561 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1621 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1681 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1741 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     1801 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc ttagggggg
//
LOCUS       DAJTNM010000250         1849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTNM010000250 DAJTNM010000000
VERSION     DAJTNM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.255"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(1..263)
                     /locus_tag="OO172_004174"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OO172_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO172_004175"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO172_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5514093.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1645)
                     /locus_tag="OO172_004176"
                     /pseudo
     CDS             complement(1175..1645)
                     /locus_tag="OO172_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1568..1819
                     /locus_tag="OO172_004177"
     CDS             1568..1819
                     /locus_tag="OO172_004177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514094.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
BASE COUNT          528 a          448 c          344 g          529 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc atctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg gctaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacgc ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatcctc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa ttacagcgcg
     1621 gacaatgaac agtaacggta gtcatggaaa acctcaaata ccagtagatt accagaaact
     1681 tctatcgatt gaagacatta ccttttgccg caataggtgg ggtaacattg gtgaaaacgc
     1741 attacgccgg gtggcagttg gaaaaaaatt atcttttttc ggctctgatc gtggtggaga
     1801 aaatgcggca ataatctaaa tagctgcgct taataccgct acacttttg
//
LOCUS       DAJTNM010000251         1829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTNM010000251 DAJTNM010000000
VERSION     DAJTNM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.256"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO172_004178"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO172_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5514095.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO172_004179"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO172_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1829)
                     /locus_tag="OO172_004180"
                     /pseudo
     CDS             complement(1292..1829)
                     /locus_tag="OO172_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          529 a          331 c          411 g          558 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaacacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt gagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttat gttgtaattt
      901 atttatggag gttttctatg gaattctttt ttattgtgac tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgata aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt aaggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt accgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctt
//
LOCUS       DAJTNM010000252         1828 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTNM010000252 DAJTNM010000000
VERSION     DAJTNM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.257"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="OO172_004181"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="OO172_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5514096.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWGLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYLADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..>1828
                     /gene="mcbR"
                     /locus_tag="OO172_004182"
     CDS             1351..>1828
                     /gene="mcbR"
                     /locus_tag="OO172_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5514097.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLW"
BASE COUNT          482 a          388 c          514 g          444 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gggctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga caggaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtgatgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatta
      721 cctcgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg cgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtgga
//
LOCUS       DAJTNM010000253         1813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTNM010000253 DAJTNM010000000
VERSION     DAJTNM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.258"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..352)
                     /locus_tag="OO172_004183"
     CDS             complement(<1..352)
                     /locus_tag="OO172_004183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514098.1"
                     /translation="MACDYRYKNRQYHCLSGSYMARSAKPRKRKPAPQRSKLLRYVVK
                     LHEDDFFDEEEAEVLRFDNFDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEI
                     ARCTEREFATIPAGI"
     gene            complement(1048..1521)
                     /locus_tag="OO172_004184"
                     /pseudo
     CDS             complement(1048..1521)
                     /locus_tag="OO172_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1449..1813)
                     /locus_tag="OO172_004185"
                     /pseudo
     CDS             complement(1449..1813)
                     /locus_tag="OO172_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          418 a          506 c          424 g          465 t
ORIGIN      
        1 tgataccggc aggaattgtc gcaaattccc gctccgtgca gcgggcaatt tccggatacc
       61 cttcgtcatc aacacgtaca aaccagaaga ccagtttttt gtttccggca tccacaaaga
      121 acggaatatt caggtctgcg caacactcaa cggcatcgtc aaaattatca aagcgcagaa
      181 cctcagcttc ttcttcgtca aaaaaatcat cttcgtgaag ttttacgaca tagcggagga
      241 gtttgcttct ttgaggtgca ggttttcgtt tgcgtggttt agctgaacgg gccatataac
      301 tacctgaaag acaatgatat tgtctgtttt tataacggta atcacaggcc atgacaagcc
      361 gcagccgtca ggccgcctac tcggggcggt ttagaacgct tatttagtac cagatcttac
      421 ttataataaa ggggcttggc agtttggata aactattgtc cttattccac taatacaaac
      481 tttttgtaaa gtatattaaa tatacttcat ttcatactga aatccccgga agtattcaat
      541 caaacaatta caccataaaa ggaagagggg gccccaatta agttgtcaag catgtttact
      601 gcgcaatcgc ttggtgctcc gtccctgtac cccgcattgg tactctggcg ctcatggggc
      661 ttccacttgc gcttctccct tcacatcggg gtgacaggtc cacgttcccc acaagagcct
      721 gaatcgactt cacgccgctt ttatgccgga tgccgcgcag gcagtaaaca tatcccacag
      781 gaacgactcc accattgttt tgacatcatc tatatgcttt cgacacatca tcagcggttc
      841 actttcgttc gtctcttcga accatacctg atcggctcac gtccgacctt ttcccatctc
      901 gctcaccacc acatctctta aatgcagcag cagtggatgg tttggtgcct gcgcctgcac
      961 gccgacaccg aggggccttc cctcatctct tgtgctgcat ggctgcacag ggactcacgt
     1021 ccccaggacg cgccttcgtg gcacacagtc atcggcatag caacagtaag caacggcagg
     1081 tttccattgc cagttttctt tgacagcagt acttcggcct cgctggatac tgtgattcca
     1141 gtaccagcga tcctttctgg cttttccact caggtagcgt ttattcagat actggtcgaa
     1201 ctcattcagt atgatgttcg acagcaacgg cgagatcaca ccgccctgtg gcatactttc
     1261 actgaccgcc cggaagagac caacatcaac acggcgtttt ccacagcagt ctcataaaac
     1321 gtgcatcact gatcctgcgg cagacggctt tcatcagcag ccggtgatgt actgtgtcga
     1381 agtagctgga caggtcacct tcaataaccc ggcgccccgt gtttccccgc agtcggtgag
     1441 ctgtaatagt acgtgatgtc tcccgcccac ttctggttcg ggccactggc gtaaaaatcc
     1501 tgcgtcagca gattttctga tacaggcagg ccgtgtgcac ggtaactgat cgggctgaac
     1561 ttctgggagg cttttgcgct cagttcctga cggtgcagac tggctgccac ggtttttacg
     1621 ttaaagtggt aaccagagca cacagttcat ccgtcaggcg tggggcaccg taacgctgga
     1681 ctgggtaaaa gccttgcgga caacgctgtc gcagtgctga cggaactgct gacgtgtgtt
     1741 tatccttgtc cgccgctgac accacgtata ccagccgctg cgggccaccc ggagtacgcg
     1801 gcacattgcc ttg
//
LOCUS       DAJTNM010000254         1802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTNM010000254 DAJTNM010000000
VERSION     DAJTNM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.259"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(195..392)
                     /locus_tag="OO172_004186"
                     /pseudo
     CDS             complement(195..392)
                     /locus_tag="OO172_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(524..>1802)
                     /locus_tag="OO172_004187"
     CDS             complement(524..>1802)
                     /locus_tag="OO172_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5514099.1"
                     /translation="PHLRTIIILKQGIVWCTSLPGNRVLLSRIPVVPDSNLLLAPAID
                     TVNRLPILLYQSQFADTRILVTISDQHIRGALNVPLKGVRYVLRVADDIIGPTGDVMT
                     LNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLIDKGFGILIFILLIACAAAFLLDRY
                     FNKSATPEEILRRAINNGEIVPFYQPVVNGREGTLRGVEVLARWKQPHGGYISPAAFI
                     PLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEGFHIGINFSASHIISPTFVDECLNF
                     RDSFTRRDLNLVLEVTEREPLNVDESLVQRLNILHENGFVIALDDFGTGYSGLSYLHD
                     LHIDYIKIDHSFVGRVNADPESTRILDCVLDLARKLSISIVAEGVETKEQLGYLNQNN
                     ITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          559 a          395 c          374 g          474 t
ORIGIN      
        1 acatcaaaat tattaatagt tgccatggta attatttcgg tgatatataa aaacaactgt
       61 gataacacca aggttgacaa ctatttgcgc aataattcct ttccttcaat gatccaggcg
      121 ttcactgcca gcaacccatt cagccgcata ctgacagtga gaaactgaaa gattattgct
      181 gatccctgtg gctgacgagt ctttctctta aatcgattaa ccagattgat cgccagccaa
      241 acaagggcat taagcaaatt tatcgtcaca agcacttctc ccagcccggt actgggtttt
      301 gcaataagga tgaccaggag ggtgatcagc ataaatgcaa tggaaaagat gatatagaga
      361 gtgaaataat ccagattatt actattgttc atgctactaa tggcaatcct gtcaaaactt
      421 tcaaacaatt atagcaaaca aaataatgaa caatagtaac aagaggcaac gagctaatta
      481 gctgtctata attacaccgc actatggcct tactgatcaa ttttcactca accacaacct
      541 tcaccttcgg tttagaaagg ataattttga ccaggtcgat gtatgtaaca ggtttataga
      601 aataataacc ctgctgaaat gtgatattat tttggttcag atagccaagt tgttctttcg
      661 tttcgacacc ttcagcgacg atactgatcg aaagtttacg cgccagatcc aatacacaat
      721 ccagaattcg ggttgattct gggtctgcgt ttacgcggcc aacgaaactg tgatcaattt
      781 tgatataatc aatatgcagg tcatgaagat aagaaagccc tgagtagcca gtaccgaaat
      841 catccagcgc gatgacaaaa ccattttcat gcagaatgtt caaccgctga accagacttt
      901 catcaacatt caatggctca cgctcggtga cttccagaac aaggtttaaa tcgcggcggg
      961 taaaactgtc acggaaattt aaacactcgt cgacaaacgt cggcgaaata atatgcgaag
     1021 cgctaaaatt aattccaata tgaaaacctt ccggcagttt actcgcgata gcgttcatct
     1081 gtctggcaac ctgattcatc aggctttgtg taagcggaac gattaatccg gatttttcag
     1141 caagtggaat aaatgccgcg ggtgaaatat atccaccgtg aggttgtttc cagcgggcta
     1201 acacctcaac tccccgcaat gtcccttccc gaccatttac cacaggttgg taaaaaggga
     1261 cgatctcccc attatttatt gcccgtcgca ggatctcttc aggcgttgcg cttttattga
     1321 aatatctatc aagcaggaag gcagcggcgc aggcgattaa cagaataaat attaaaatcc
     1381 caaagccttt atcgataagt ctgtagaagc taaagagtgg cggcgggtta aagataatag
     1441 tgaaatgata ttttgtggag tgaaccttct cggtataggg ataatgtcca ttaagcgtca
     1501 tcacatcacc cgttggtcca ataatgtcat ccgccacgcg taatacatac cttacccctt
     1561 tcaagggtac attaagcgcc ccgcgaatat gttgatcact tatcgtaacc aaaatgcgcg
     1621 tatctgcaaa ttggctctga tagagcagga taggtaatct attaacggtg tcgattgccg
     1681 gagccaacag taaattactg tccgggacaa caggaatacg agacagcagg acccgattcc
     1741 caggcaggga tgtacaccag actattccct gtttgagaat gatgattgtg cgcaggtgag
     1801 gt
//
LOCUS       DAJTNM010000255         1796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTNM010000255 DAJTNM010000000
VERSION     DAJTNM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.260"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(178..579)
                     /locus_tag="OO172_004188"
                     /pseudo
     CDS             complement(178..579)
                     /locus_tag="OO172_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1008..1796
                     /gene="virF"
                     /locus_tag="OO172_004189"
     CDS             1008..1796
                     /gene="virF"
                     /locus_tag="OO172_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5514100.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          561 a          329 c          331 g          575 t
ORIGIN      
        1 cttccttatg ttatatttta gtaaagaatt tattttttac attatagtcg gctaaatgaa
       61 gtttactgta aaaaaacgat acttttttcg taagcgatga gatgtggagc cagaaaaatg
      121 gctgcgttac gtcattgaac tgtccttgac ccacgatatt cagacagtcg ttgtatctta
      181 agttgacatt acccgctgac gtcgatattc ccttggtgag agataaccca acgcactatg
      241 cggatggttt tcattgtaat gctcgatcgc cactgcaagg ttatgcaatg ccgttcttgg
      301 tttcggcatg aacgcgatgc agtcttcctt catcgttttc acgaaccgtt ctgctattcc
      361 attactctga gggctactga ctgccgtcgt gcagggctcc agattcaact ctctggcgaa
      421 ctgccgtgtt tcatgagccg tataagccga accgttatcg gccactggat tggccgttcc
      481 ggtcctttat cgccatatca ctcctgacat gacatcctgc accgttgctt tgtcataatc
      541 tcccgtgcct gctgcccagt ctatggcttc acgatcgcaa tatggaacgt aacgctgtca
      601 tcgggagaat cgtcaatgcg gcattcggtt aacgcttacc aatatcaaca ccaaccggat
      661 ttgtggagcc tccagtctga aggggcttta tgcgttccgt ataggatatt atgatgctgg
      721 agttttgcga agctccactc ctttccgaac atttacacag atattgccta agaaaagtga
      781 aaccataata tggttatagt ccctttcagt gcaaatactt agcttgttgc acagagaaat
      841 agaagctgca taagctcttt cttcaaaaaa tgtaaataaa gttaaatata ggaaaaatta
      901 cttaatctat cttaataacg gaaagatttt gtatacaatc actgttacag aaatttctta
      961 gttactctgt aaacactaaa tatagtttgg tatattctgt tgaatttatg atggatatgg
     1021 gacataaaaa caaaatagat ataaaggttc gcttgcataa ctatattatt ttatatgcaa
     1081 aaaggtgttc aatgacggtt agctcaggca atgaaacttt gactatcgat gaagggcaaa
     1141 ttgcttttat agagcgaaat atacaaataa acgtctccat aaaaaaatct gatagcatta
     1201 atccatttga gattataagc cttgacagaa atttattatt aagcattatt agaataatgg
     1261 aaccaattta ttcatttcaa cactcctatt ctgaggagaa aagggggtta aacaaaaaaa
     1321 tattcctcct ctctgaggag gaggtttcta tcgatttgtt caaatctata aaagagatgc
     1381 ctttcggcaa aagaaagatc tatagtttag cttgcctttt atcagctgtt tctgatgagg
     1441 aagctttata tacttcgata tcgatagctt cttctcttag tttttctgat cagataagga
     1501 agattgttga aaaaaacatc gagaagagat ggcgtctttc tgatatttca aataacttga
     1561 atttatcaga aatagctgtt agaaaacgat tggagagtga aaaattaaca tttcaacaaa
     1621 tccttcttga tattcgcatg catcatgcag caaagctttt attgaatagt caaagctata
     1681 ttaatgatgt atcaagactt atcggaatat caagcccatc ttattttata aggaaattta
     1741 atgaatatta tggtataact ccaaagaaat tttacttata tcataaaaaa ttttaa
//
LOCUS       DAJTNM010000256         1788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTNM010000256 DAJTNM010000000
VERSION     DAJTNM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.261"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..561
                     /gene="ssuE"
                     /locus_tag="OO172_004190"
     CDS             1..561
                     /gene="ssuE"
                     /locus_tag="OO172_004190"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5514101.1"
                     /translation="MAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAPEDLLY
                     ARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVVLPLAT
                     GGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPHLDTAL
                     ETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            554..1513
                     /gene="ssuA"
                     /locus_tag="OO172_004191"
     CDS             554..1513
                     /gene="ssuA"
                     /locus_tag="OO172_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5514102.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTIGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1510..>1788
                     /locus_tag="OO172_004192"
     CDS             1510..>1788
                     /locus_tag="OO172_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5514103.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAA"
BASE COUNT          404 a          502 c          467 g          415 t
ORIGIN      
        1 ctggcgggta gtcctcgctt tccttctcgc tccagctcct tgctggaata tgcgcgggaa
       61 aaactaaatg gcctagatgt agaggtttat cactggaatc tgcaaaactt cgccccggaa
      121 gatctgcttt atgctcgttt cgatagtccg gcactcaaga ccttcaccga acagctgcaa
      181 caggctgatg ggctgattgt cgccacgcct gtgtataaag ccgcctattc cggcgcgctg
      241 aaaaccctgc tcgacctgct gccagagcgc gctttgcaag gcaaagtggt gctaccgctg
      301 gcgacgggcg gtaccgtggc ccatctgctg gcggtcgatt atgcccttaa accagtttta
      361 agcgcactga aagctcagga gatcctgcac ggcgtgtttg ccgatgactc acaattaatt
      421 gattaccatc acagacccca gttcacgcca aatctgcaac cccatcttga taccgcgcta
      481 gaaactttct ggcaggcatt gcaccgccgc gatgttcagg ttcctgacct tctgtctctg
      541 cgaggtaatg cccatgcgta acatcattaa actggcgctg gcgggattgc ttagcgtctc
      601 tacttttgcg gttgctgcag aatcctcgcc tgaagcgtta cgtataggct atcagaaagg
      661 cagtattggt atggtgctgg caaaaagcca ccagttactg gaaaaacgct atccgcaatc
      721 aaaaatctcc tgggtggagt tccccgcggg tccgcaaatg ttggaagcgt taaacgttgg
      781 cagtattgat ctcggcagta ccggggatat tccgccaatc tttgcccagg ctgccggggc
      841 tgatttggta tatgtgggcg tcgagccacc gaagcccaaa gccgaagtga ttctggtggc
      901 agaaaacagc ccgatcaaaa ccgtagccga tcttaaaggt cacaaagttg cctttcagaa
      961 aggttccagt tcacacaacc ttttactgcg tgcactgcgt caggccggac ttaaattcac
     1021 cgatatccag cccacttacc tgacgcctgc cggtgcccgc gccgcgttcc agcaaggtaa
     1081 cgttgacgcc tgggctatct gggatcccta ctactccgct gcgttattac agggcggcgt
     1141 gcgggtgctg aaagacggca ccgatctcaa tcaaactgga tcgttttatc tggcagctcg
     1201 tccctatgca gaaaaaaacg gcgcttttat tcagggcgta ctggcaacct ttagtgaggc
     1261 cgatgcgtta acccgcagcc agcgcgaaca aagcatcgct ttactggcaa aaacgattgg
     1321 cttaccggca ccggtgattg cctcttactt agatcatcgc cctcctacca ccatcaaacc
     1381 ggttaacgcc gaggttgccg ccttacagca gcaaacggca gatctgtttt atgaaaaccg
     1441 tctggtgccg aaaaaagtcg atattcgcca gcgcatctgg cagcccactc aactggaagg
     1501 aaaacaatta tgagtctgaa tatgttctgg tttttaccga cccacggtga cgggcactat
     1561 ctgggaacgg aagaaggttc acgcccggtt gatcacggtt atctgcaaca aattgcgcaa
     1621 gcggcggatc gtctgggcta taccggtgtg ctgatcccaa cggggcgatc gtgtgaagat
     1681 gcgtggctgg ttgcagcgtc gatgatcccg gtgacgcagc ggctgaagtt tcttgtcgcc
     1741 ctgcgtccca gcgtaacctc acctactgtt gccgcccgcc aggccgcc
//
LOCUS       DAJTNM010000257         1779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTNM010000257 DAJTNM010000000
VERSION     DAJTNM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.262"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..448
                     /locus_tag="OO172_004193"
     CDS             <1..448
                     /locus_tag="OO172_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5514104.1"
                     /translation="GWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGGI
                     FYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTWN
                     GPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            451..984
                     /locus_tag="OO172_004194"
     CDS             451..984
                     /locus_tag="OO172_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5514105.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1013..1540)
                     /locus_tag="OO172_004195"
     CDS             complement(1013..1540)
                     /locus_tag="OO172_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5514106.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1542..1779)
                     /locus_tag="OO172_004196"
                     /pseudo
     CDS             complement(1542..1779)
                     /locus_tag="OO172_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171265.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          498 a          391 c          399 g          491 t
ORIGIN      
        1 tggctggaac tggaatagcg gtgtttatga tgcaaatatc agcggtgcat caacattaat
       61 cctccacttc aataagaata cggggagctg ccctgctgta cagttccgtg tgaattataa
      121 gaacggcggt attttttatc gttcagcgcg tgatggttat ggatttgagg ctggctggtc
      181 agagttttac accacaacac gcaagccatc agcgagagat gttggcgcat atacgcaggc
      241 agaatgtaac tcaaggttta ttacaggtat tcgccttggt ggtctgtcat ctgttcggac
      301 atggaatggt cccggctggt ctgacaggtc aggttatgtt gttaccggtt ccgttaactc
      361 aaacagagat gaactaattg atacaactca ggcaaggcca attcagtatt gcattaatgg
      421 gacgtggtat aacgcgggga gtatttaacg atgatgcact taaaaaacat ttctgctggt
      481 aatcctgaaa caaaagagca ataccagcta acgaaacaat ttaacatcaa atggctttat
      541 acagaggatg ggaaaaactg gtatgaggaa caaaagaact ttcagcctga tacgttgaaa
      601 atggtctatg accacaacgg cgttattatt tgtattgaaa aggatgtttc agcaattaat
      661 ccggaaggcg caagcgtcgt tgaggttcct gatattacag caaatcgccg ggctgatatt
      721 tcaggtaaat ggatgttcaa agatggcata gtgataaagc gaacttatac cggggaagag
      781 cagaggcaac aagtggaaaa tgaaaagcaa agcctgttgc aacttgtcag ggataaaacc
      841 cagctatggg actcacagct acgactgggc atcatttccg ccgggaataa gcagaaatta
      901 accgagtgga tgctctacgc gcagaaggtc gaatccacag acacctccag tctgccagta
      961 acgtttcccg aacaaccaga atgagagaag gcccgctatc gggccttaat ttttactctg
     1021 gtttttgtgg ccattctggc tttgccgtat ccacacggct gaccagaaca ctgtagcgtt
     1081 cccatgactc cagtcgtgcg cattcctcat ctgttgccat attcagcctg acagagcgct
     1141 ccagcggctg gatgactgat tcagcttcgg aaagcaatgc ggctttttgt gattcggcct
     1201 gttgctgctg ttcgtctgcc gtataaatcc gcttaaccac agctccgtcc ttaaacatcc
     1261 acttcccgga atcatcggca cgacgatttg ctgttatatc ggggacctca acgacgctaa
     1321 agccttcagg attaagcgtg gaggcatctt tagtgatggc gacaataata ttattttcat
     1381 cgtaaacaat ctttattgta tctggctgaa agttcttcac ttccccatac cagttttttc
     1441 cgtcttctgt ccacaaccag attacgtcaa aattctttgt taattcgtac tgttccagtg
     1501 ttttagcgtt acccgcttta atgttcttta agtgcatcat attaaacgct cgctacatta
     1561 taccaagtgc catttatata cttttgaatc ggtctgtaat aaacacccgc aatattatcg
     1621 gcagagttga acctgtatcc tgaacattaa taccagacaa tacatgacct gaagggcact
     1681 gaaaattcca tgtttgccag ttgttcactc cataatattg ctgtgaacca agtcgaacat
     1741 ctttcacata tctggaatca aaattgccat agttgccgg
//
LOCUS       DAJTNM010000258         1626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTNM010000258 DAJTNM010000000
VERSION     DAJTNM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.263"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..742)
                     /gene="ipaH3"
                     /locus_tag="OO172_004197"
     CDS             complement(<1..742)
                     /gene="ipaH3"
                     /locus_tag="OO172_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5514107.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIR"
     gene            1484..>1626
                     /locus_tag="OO172_004198"
     CDS             1484..>1626
                     /locus_tag="OO172_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5514108.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLL"
BASE COUNT          470 a          274 c          379 g          503 t
ORIGIN      
        1 ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc ggatcaaggc
       61 taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa aatatctcgg
      121 tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga agattagtac
      181 ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc tggttatttc
      241 ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc tgattgttat
      301 ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt tggttgttat
      361 ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg tgattgtcac
      421 aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg gcattctgag
      481 taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac agatttaaac
      541 gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg gagaccgctt
      601 cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct gaaaagtaat
      661 cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa aagttattat
      721 ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt tgttaaaaga
      781 aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac ttttttatta
      841 taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa ctgtgaatag
      901 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
      961 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
     1021 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
     1081 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
     1141 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
     1201 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
     1261 aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg tttgttgacg
     1321 aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
     1381 tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt ttttaacatt
     1441 cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
     1501 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
     1561 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
     1621 tttact
//
LOCUS       DAJTNM010000259         1540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTNM010000259 DAJTNM010000000
VERSION     DAJTNM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.264"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            122..397
                     /locus_tag="OO172_004199"
     CDS             122..397
                     /locus_tag="OO172_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514109.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            497..>1540
                     /locus_tag="OO172_004200"
     CDS             497..>1540
                     /locus_tag="OO172_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5514110.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEV"
BASE COUNT          459 a          361 c          363 g          357 t
ORIGIN      
        1 gtagtgaaat ctgtgccgaa aaagaccaga tggcacacga actttaatta actgactatc
       61 cgaaactgaa tttatgccag caatggcagg gattcgctca accttaatta aggagaaaaa
      121 catgattacc agttatgaag ccactgttgt aactactgat gacattgttc acgaggttaa
      181 tctggaagga aagcgtattg gctacgtgat taagacagaa aataaagaaa ccccattcac
      241 tgtggttgat atcgacggtc catcaggcaa cgttaaaaca cttaacgatg gcgtaaaaaa
      301 aatgtgtctg gtgcacatag gaaagaatct gcccgcagaa aaaaaagccg aatttctggc
      361 aactctgatt gcaatgaaat taaaaggtga aatctgaaaa aaagaaagcc tgcacactgt
      421 gcaggcctga gtgaagaacc tgggacattt attcatcact cgcagtaatt ttaatctgag
      481 ttgaggttaa aaaacaatga acaccgataa acaagtttac ccactgtatt acgaagcaaa
      541 aaatgacaaa gtaagaaaac gtctcggtat taaaggcggt ttttactggg ctgaagcgaa
      601 aaaattatcc attgccatct cccgtggtgc tgttgcgatt gacgatgcag gctacgatga
      661 agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt gttgatgacc tcccgccaga
      721 aggcgtattt gatacggaat tctgcaaccg ttacgaaaaa ggcggggaag atggcatcac
      781 aatggtattt atcgcatctt ctccctctgt tcaggacaaa ccggccagca ctgacaatac
      841 caacgtcaac ggcgaagaca tgacagaaat tgaggagaac atgctcctgc cagtttccgg
      901 tcaggaactg cccattcgct ggcttgcgca acacggcagc gaaaaaccgg taacgcacgt
      961 ttcacgcgac gaactccagg cattacacat tgctcgggat gaagaactac cagctgttac
     1021 tgccctagct gtttcccaca aaaccagcct gctcgatccg ctggaaattc gcgatctcca
     1081 caaactggtg cgtgatactg acagagtttt ccctaatcca ggcaattcaa gcctggggct
     1141 gatgactgct tttttcgaag catacatgga cgcagactac accgatcgcg gtctgctgac
     1201 aaaagagtgg atgaaaggaa atcgtgtttc acgcatcacg cgcacggctt ccggcgctaa
     1261 tgctggcggc gggaacctca ccgatcgcgg cgaaggtttc gttcacgatc tgacgtcact
     1321 ggcgcgcgac gtagccactg gcgtactggc tcgttcaatg gacgtggaca tttataacct
     1381 tcatccggca cacgctaaac gtgtcgagga aattatcgct gaaaataaac cgcccttttc
     1441 tgttttccgc gacaaattca tcaccatgcc tggcgggatg gattattccc gcgccatcgt
     1501 ggttgcgtca gtgaaagaag caccgattgg tatcgaggtc
//
LOCUS       DAJTNM010000260         1525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTNM010000260 DAJTNM010000000
VERSION     DAJTNM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.265"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            499..1525
                     /locus_tag="OO172_004201"
                     /pseudo
     CDS             499..1525
                     /locus_tag="OO172_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          429 a          345 c          389 g          362 t
ORIGIN      
        1 ttgggtaata aaaaccatag gttggataat ttttcaatct ttgataattg atttccgatt
       61 atgaaaaata aaactgagaa ggagaaaata attgagtaac taaaagtact caggccataa
      121 gtatgcatga aaattgatgg tgatattgat atgtagcaaa atagagaact gagtattaat
      181 gagacaagta tagtgccgca gataaactct ttggtcacaa aggatgcact gttttgacat
      241 attactttct catttgcatg agttatcttt ggtgataata acattattat aatactgaac
      301 agcatcataa caagaaatat aacattccag ttataatatt caataatcaa tgcgccgccg
      361 agaggtgcta ctaatggtat tactgcgctt acagatgata tatcagaaag cgcccgtttt
      421 agttttaatg gagggtgatc ttacccacca atagtggaca caggactaag tgagtaaact
      481 ctcaaccaga ggtgactcat gacaaaacca gtatcaatca gcaagaagcc ccgtaaacaa
      541 catacgcctg aatttcgtaa cgaagccctg aaactcgctg aacgcatcgg tgtggccgcc
      601 gcagcccgtg aactcagcct gtatgaatct cagctttatg cctggcgcag taaacagcag
      661 caacaaatga gttcgtcaga gcgcgaaagc gaactggccg ctgaaaatgt ccgccttaaa
      721 cgacaactgg cggagcaggc tgaggaactg tccatcctcc aaaaggccgc gacatacttc
      781 gcgaagcgcc tgaaatgaag tatgtcttta tcgaaaatca tcgggcagag ttcagcatca
      841 aagcgatgtg tcgtgtactt cgggttgccc gcagcggctg gtatgtctgg ctcaggcgtc
      901 gtcaccagat gagcctgcgc caacagtttc ggctcacctg cgatgccgct gtccataagg
      961 cattctttga ggcaaagcag cgatacggtg ctccccgcct tgctgacgaa ctgccggagt
     1021 tcaatattaa aaccattgcc gccagcctgc gtcgtcaggg gctgcgggcg aaagccagcc
     1081 ggaagttcag cccggtcagc taccgtgcac atggcctgcc cgtattggag aatctgctgg
     1141 agcaggactt cagcgccagt ggcccgaaac cagaagtggg cgggtgacat cacgtacttg
     1201 cgtaccgatg agggctggtt gtatctcgca gtagtcatcg acctgtggtc acgcgccgtc
     1261 attggctggt cgatgtcacc gcgaatgaca gcacaactgg cctgtgatgc actgcaaatg
     1321 gcgttgtggc ggagaagacg cccggaaagc gtcattgttc atacggaccg tggtggtcaa
     1381 tactgttcag gggattatca ggcaatgctg aagcgacaca acctgcgtgg cagtatgagt
     1441 gcgaaaggct actgttatga caatgcctgt gtggaaagct tctttcattc gctgaaggtg
     1501 gaatgtatcc acggggaacg cttta
//
LOCUS       DAJTNM010000261         1503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTNM010000261 DAJTNM010000000
VERSION     DAJTNM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.266"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..1071)
                     /locus_tag="OO172_004202"
     CDS             complement(<1..1071)
                     /locus_tag="OO172_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5514111.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLS"
BASE COUNT          451 a          238 c          326 g          488 t
ORIGIN      
        1 ggaaaggcgg tcaaggaacg cggagaatgc ccgggaattc tcctcggtgg aaaaagaagt
       61 ccattgtgta gaaacctccc gacgatctgc ttcaggaaac caggctgcca cagtttcggg
      121 aagagggggg atctgaaagt tctgcccccc ttcagtgatg actctgatat ggcgcgaatg
      181 taaattttgc ggcaacatct ggcgtaaaaa cagtctgttt tggtctgaca gggaaatacg
      241 aaactgaggc ccgttaaaat ttggaaccga agtttgctgt attaagaata gcagagtttg
      301 atctgataag gggttatttg caatctcaat agttatatga ggatctaaac gaaaaagatt
      361 tgtcgggatt gtagttattt ggttgttttc aaaataaaaa aatcttctca tgataatatt
      421 ttcttggatg tttggtaagt tgcttaatct attgctatat gcaaaaagaa gttgcagtct
      481 cctaggtaaa gggggcagtt cagtaagatg gttgtgcgca accttaagta tcttaagatt
      541 agaaggcaga gatggaagag tttgcagttc gttgttccta acctcaagtt tttttaaatt
      601 caagggtaat gaaggcagtg tggataacct attatactct gcgcttagtg atttgatggt
      661 gtctggtaat aacggcagat tctctaattg attttcggat acatccaaag ttattagata
      721 atgtggtaat gcagatatat gtgttaagaa attatctctt gcaataagtt ctttaatatt
      781 ttcaggtaaa tcagtaagga tggttaattt attacagctc acatcaagct ttttaatgca
      841 agcagggagt acaggtaacg aggaaagcgc caaggctcgc aggtccagtt tatgcccctt
      901 agaggtaaga catgcacgaa gtcttcttac cgcctcagta cgctgttctt ccggtaaccc
      961 ctgtttctcc caatcatccc attcggacaa ataatggttg ggggatgtaa ttgtgctgat
     1021 atgtggaaat aaacaggatg aaatatttct gagcatatta atttctcgca tatggatatg
     1081 gaattagatt gtgaataaat tgtatttaat gtatctctcc taatgaggag ggtgtttcag
     1141 aatattcgcc ataagggaat gagtgttata tcatgctttc taccctggta agtatatttg
     1201 cttttttcgt atatttggtt catctttttt tgatgtttta catattcaca atgcagagga
     1261 tttataccag aagacattag tcttgagtaa gatactacaa catctccaac tattttagcc
     1321 agcctgacta cttgacttgg taatatttgt tgctaataat catgtataaa ataagcttat
     1381 atacatagta acttcagcaa aggatttagt aatgatccaa atcatatctc aatgtactgt
     1441 ttttttcttt aaaaaaacag tacattctat tttataatga ctgtgtattc ccatggaagg
     1501 aga
//
LOCUS       DAJTNM010000262         1479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTNM010000262 DAJTNM010000000
VERSION     DAJTNM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.267"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="OO172_004203"
     CDS             complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="OO172_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5514112.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPIFTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
     gene            complement(1263..>1479)
                     /locus_tag="OO172_004204"
     CDS             complement(1263..>1479)
                     /locus_tag="OO172_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5514113.1"
                     /translation="AEEQKYEMLENEYSQRVADRLKASGLSGDADAEREAGAQVMRET
                     EQQIYRQLTDEVLALRLSENGSRLHHS"
BASE COUNT          398 a          301 c          324 g          456 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgtgagtcg
       61 acaatggatt ccctgaaatg ttaacattcg taaaaatggg caatgtgatt atattttcgg
      121 gtaatgaagt tagtaggtta ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg
      181 ataatctgcg tagttgattt tcacctacct tcaaatattt taagctctga ggaaattctg
      241 gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag
      301 gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg
      361 gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg
      421 gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg
      481 tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt
      541 caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg
      601 gtggtaaaga acgtagtttc agtaagctta aattgagaac aggctcccgg gtatccagac
      661 atattttcaa taatcgaacc gcctggatgc gattttctgt tgttgttcct tccctagccc
      721 agtcatccca gatgcggtgg taatctctgg tacactgttc ttctaccgat aaacgagata
      781 tacatgataa gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt
      841 aacccctcac tattcatatc ttagtctatt aatgcaaata aaacgagaaa gtctttaaaa
      901 aaacaatcct agagctttat cggcgttttc attccggctg gaggaatatg ttcctgtctt
      961 tccagtaacc gtcgattgtt atatcagtct acccatgtta atgtgaccag ttctactttc
     1021 atccagtttg gattgatctt tatcccctat attttcaaac aatttttatc atttaactca
     1081 ttactgtctc gatattccca tagcaaacga tctggttggt tatcccctca aaaaagtcag
     1141 ttgcacgccg taaatccctg ccggtattga gcgaggattt acgggttgtt gttaaggtcc
     1201 gtaatcgggt cacagttttt ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt
     1261 gattatgaat ggtgcagtcg tgagccgttt tcagacaatc gcagggccag tacctcgtca
     1321 gtcaactgac ggtaaatctg ctgttcagtc tcacgcatca cctgtgcacc ggcttccctc
     1381 tccgcatccg catcaccgct cagacctgat gctttcagcc ggtcagccac cctctgagag
     1441 tactcattct ccagcatctc atacttctgc tcttctgcc
//
LOCUS       DAJTNM010000263         1414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTNM010000263 DAJTNM010000000
VERSION     DAJTNM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.268"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            113..1414
                     /locus_tag="OO172_004205"
                     /pseudo
     CDS             113..1414
                     /locus_tag="OO172_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
BASE COUNT          322 a          316 c          363 g          413 t
ORIGIN      
        1 cagaaaaata ttttccggtg aagtttatct tccctgaaaa aattcgttgt caggataagg
       61 acaatcaata aaggacttct gtatgagtca tacagaaaga acaggatttt aaatgaataa
      121 gaaatcgtta tggaagctaa ttctgatatt agcgatccca tgtattattg gttttatgcc
      181 agctccggca ggattaagcg aactggcgtg ggtgcttttt ggtatttacc tggcggccat
      241 tgtgggtctg gttatcaagc ctttcccgga acctgtcgta ctgttaattg ccgttgctgc
      301 ctcaatggtg gtggtcggta acttatccga cggtgcgttt aagaccaccg ccgtattaag
      361 cggttactct tcaggtacta cctggctggt gttctcggcg tttaccttaa gcgccgcatt
      421 tgtgaccacc ggtttaggta aacgtattgc ctatctgctg attggtaaaa tcggtaacac
      481 catgctgggt ctgggttacg ttacggtatt cctcgatctg gtactggctc cggcaacacc
      541 gtctaacacc gcgcgtgcgg gcggcattgt gttaccgatc atcaacagcg tggcggtggc
      601 tttggggtcc gaaccggaaa aaagtccgcg tcgtgtcgga cattacctga tgatgtccat
      661 ttacatggtc accaaaacca ccagctatat gttctttacc gcaatggcgg ggaacattct
      721 ggcgctgaaa atgatcaacg acattctgca cctgcaaatt agctggggtg gatgggcgct
      781 ggccgccgga ttgccggatt gccggattgc cggattgccg gattgccggg catcattatg
      841 ctgctggtca ccccgctggt aatttacacc atgtatccac cagaaattaa aaaggtggat
      901 aacaaaacca tctctaaagc gggccttgcc gaactgggac cgatgaaaat ccgcgaaaaa
      961 atgctgctcg gtgtctttgt gctggcgcta ctgggctgga ttttcagtaa gtctctgggg
     1021 gttgatgaat ccactgtggc aatcgttgtt atggcgacca tgctgctgct gggtatcgtt
     1081 acctgggaag acgtggttaa aaataaaggc ggctggaata ccttaatctg gtacggcggt
     1141 attatcggct taagctcctt attatcgaaa gttaaatttt tcgaatggtt agctgaagtc
     1201 tttaaaaata acctggcatt tgatggtcac ggtaacgttg ctttcttcgt tattattttc
     1261 ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg cctatatcgt tgctatgtta
     1321 cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt taatgttaac cgcgctggca
     1381 ctgttgttct ccaactccta tggcggcatg gtta
//
LOCUS       DAJTNM010000264         1402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTNM010000264 DAJTNM010000000
VERSION     DAJTNM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.269"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..1003
                     /locus_tag="OO172_004206"
     CDS             <1..1003
                     /locus_tag="OO172_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5514114.1"
                     /translation="RENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWS
                     YAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIV
                     DINTRAILSAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFS
                     QRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEH
                     FYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRI
                     IFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            1011..>1402
                     /locus_tag="OO172_004207"
     CDS             1011..>1402
                     /locus_tag="OO172_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514115.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTG"
BASE COUNT          390 a          280 c          339 g          393 t
ORIGIN      
        1 tcgggaaaat gtacagacat ttacccgtaa aatagcgatg gtaaacgatt ttgacagcag
       61 ctttggctac cgcaatctgc gcatccataa gtctgccatt tgtgaacctt tgcaggggga
      121 aaacgcctgg agttacgcag aaaaactgaa actggcgatt tccagtatcc gtgaccatat
      181 gttctccgag tttattttct gtgatgacgc gaaactcaac gaaatagaag agaatatctg
      241 gattgcgcgt aatattcgcc atgcaatgga aattggcgaa ctattcctcg tctatcaacc
      301 gatcgttgat attaacaccc gcgccattct gagcgcggag gcgttgtgcc gttgggtttc
      361 tgcggagcgg gggatcattt caccgctgaa gttcattacc attgctgaag atatcgggtt
      421 tatcaatgag ctgggttatc agattattaa aacggcaatg ggtgaattca gacattttag
      481 tcaacgtgcg tcgctgaagg atgatttctt actgcatatt aatgtttcgc cctggcagtt
      541 aaacgaaccg cactttcatg agcgttttac caccatcatg aaagaaaatg gcctgaaggc
      601 gaacagcctc tgtgttgaga tcactgaaac cgtgatcgag cgaattaatg aacattttta
      661 tctcaatatt gaacaactgc gtaaacaagg ggtacggata tcgattgatg actttggcac
      721 cggtttgtca aacctgaaac gtttttatga aattaatcca gacagcataa aggtggactc
      781 gcaatttacc ggcgatattt tcggtactgc gggaaaaatt gtgcgcatta ttttcgatct
      841 ggcacgctat aaccggatcc cggtgattgc ggaaggcgta gagagcgaag acgttgcgcg
      901 cgaattaatt aaattaggat gcgttcaggc tcaggggtat ctgtaccaga aacccatgcc
      961 attctccgcc tgggataaaa gtggaaaatt agtaaaagag tagtttacgt atgtccagaa
     1021 tcaataagtt cgtacttaca gtcagtctgc tgatttttat catgatttca gcagttgcct
     1081 gcgggatcta cactcaaatg gtaaaggaac gggtgtatag cctgaaacag tccgttattg
     1141 atactgcttt tgcggtggca aatattgctg aatatcggcg tagcgtggca attgatctta
     1201 tcaacacgct aaatcccacg gaggaacagc tgttggttgg tttgcgcaca gcttacgccg
     1261 actcggtttc cccctcttat ttgtacgatg tcggtcctta tctgatttcc agtgacgaat
     1321 gtattcaggt aaaggagttc gagaaaaatt attgtgcaga tattatgcag gttgtgaagt
     1381 atcgacatgt caaaaataca gg
//
LOCUS       DAJTNM010000265         1396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTNM010000265 DAJTNM010000000
VERSION     DAJTNM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.270"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..332
                     /locus_tag="OO172_004208"
     CDS             <1..332
                     /locus_tag="OO172_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514116.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILIR
                     EIKGSSH"
     gene            332..679
                     /gene="tnpB"
                     /locus_tag="OO172_004209"
     CDS             332..679
                     /gene="tnpB"
                     /locus_tag="OO172_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5514117.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            699..>1396
                     /locus_tag="OO172_004210"
     CDS             699..>1396
                     /locus_tag="OO172_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514118.1"
                     /translation="MDTSLSHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPILPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVAGKYADYLPLYRQSEIYRRQ
                     GVELSRATLGRWTGA"
BASE COUNT          328 a          389 c          385 g          294 t
ORIGIN      
        1 ggcctaattt tccttatgaa tttaaaatcg ctttagtgga gcagtcactg cagcccggag
       61 cctgtgtggc gcagatcgcc cgtgaaaacg gaatcaacga taacctgctc ttcaactggc
      121 gccatcaata ccggaaaggt ggcctgctgc cttccggaaa aaatatgcct gcactgcttc
      181 ccgtgacgtt aacgccggag cagataaata caccgtccga cagtctgtgc tgtgagctgg
      241 ttctgccggc cggaactctc aggcttaaag gtaaactgac gccggcgtta ttacagatac
      301 ttatccgcga aataaaaggg agcagccact gatgatatct ttccctgcag gttcgcgtat
      361 ctggctggtt gcaggtatca ccgatatgcg aaatggcttt aacggcctgg cctcaaaagt
      421 tcagaacgtc ctgaaggatg acccgttctc cgggcatctg ttcatcttcc gcggacgccg
      481 gggtgaccag ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct ttaccagacg
      541 cctggagcgg ggccgcttcg tctggccggt cacccgtgac ggcaaggtgc accttactcc
      601 ggcccagtta tccatgcttc ttgagggcat cgactggaaa cacccgaaac gaacggaacg
      661 cgctggcatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca ctttctcatg
      721 agaatgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc cagatggcga
      781 aatacaaccg cctgctctca cagcgggtgg cggcttatgc ttccgaaatc aaccggctga
      841 aggcgctggt tgcgaaactg cagcgcatgc agttcggtaa aagctcagaa aaacttcgcg
      901 caaaaaccga acggcagata ctggaagcac aggagcgaat cagcgcactt caggaagaaa
      961 tggcggaaac gctgggtgag caatatgacc cgatactgcc atctcccctg cgccagtctt
     1021 cagcccgtaa accgttacct gcctcacttc cccgtgaaac ccgggtcatc cgtccggaag
     1081 aggaatgctg cccggcctgt ggtggcgaac tcagttctct gggatgtgat gtgtcagagc
     1141 agctggagct tatcagcagc gcctttaagg ttatcgaaac acaacgtccg aaactggcct
     1201 gttgccggtg tgaccatatc gtgcaggcac cggtgccttc aaaacccatt gcacgcagtt
     1261 atgccggagc ggggcttctg gcccatgttg tcgctggaaa atatgcagac tatctgccgt
     1321 tataccgcca gtcagaaata taccgtcgtc agggcgtgga gctgagccgt gccacactgg
     1381 ggcgctggac aggtgc
//
LOCUS       DAJTNM010000266         1391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTNM010000266 DAJTNM010000000
VERSION     DAJTNM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.271"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            443..1192
                     /locus_tag="OO172_004211"
     CDS             443..1192
                     /locus_tag="OO172_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5514119.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          389 a          283 c          290 g          429 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtaaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa
      241 cttaattcta actgtttgtt ctggatgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcgcacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcctctata aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt
      481 attttcttta aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga
      541 cctttactac ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc
      601 acaaatcgga agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt
      661 gcgaaatact gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt
      721 ggcaaatgta ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt
      781 gcataaatta ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa
      841 agaatattat atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa
      901 agaacaggat acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg
      961 ggcaacagca cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg
     1021 gggttatgaa ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga
     1081 cgcagcacgt attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca
     1141 ggcacaatta caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta
     1201 gccagtacgc cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact
     1261 ggctcttttt cagggctgtg gggtatttcc agggcctctc ctttaccact ctcgggaagg
     1321 ccctttccct tcttgtcggt aatttcacta ggctgtgtcc cttaattacg taagcgttga
     1381 taaaagagtc g
//
LOCUS       DAJTNM010000267         1356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTNM010000267 DAJTNM010000000
VERSION     DAJTNM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.272"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            193..462
                     /locus_tag="OO172_004212"
     CDS             193..462
                     /locus_tag="OO172_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514120.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..685
                     /locus_tag="OO172_004213"
                     /pseudo
     CDS             459..685
                     /locus_tag="OO172_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="OO172_004214"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="OO172_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5514121.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          389 a          263 c          333 g          371 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagccgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat ggggac
//
LOCUS       DAJTNM010000268         1326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTNM010000268 DAJTNM010000000
VERSION     DAJTNM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.274"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            200..739
                     /locus_tag="OO172_004215"
     CDS             200..739
                     /locus_tag="OO172_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5514122.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(965..1288)
                     /locus_tag="OO172_004216"
     CDS             complement(965..1288)
                     /locus_tag="OO172_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR5514123.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
BASE COUNT          462 a          302 c          244 g          318 t
ORIGIN      
        1 ggattgttaa caaatgaaaa tactcaattc gaatctcata acaaacatat catcaacaat
       61 aagaaatcac cttctaacat catccaaaaa tataaaaaca cacccatcac caaacatgga
      121 cgtaacaagt catcgtcgtt tattccagaa agacggaata ggattgagcc aaacaggaaa
      181 agcaacacaa cttgtcatta tgtcgcatgg tggatggaaa gagatctccc atccacaaac
      241 actattcacg cgtcaaaaag gcgatggatg gactgtagtc cccaaaaacc taagaattga
      301 tttttataca aaagacaacg acttcacaaa gggactaagc gttctttcag aggtaaacaa
      361 acgccatact gaagctcaaa aagggttaac accatcacta aacataagca aagatgacct
      421 aaaagttcta gcgaacgcgc gcaatatacc tcaatcaaaa ttagaagaag aaatgatgtc
      481 acaagccata tatagaaaag aatacgcaac aagtaatgag aagataaaaa actatgctct
      541 atattaccat gaacaagccc aaaatattat acaaagacat caagatggct taggaaacaa
      601 aaatattgat atagcattta taactgataa aaatcataag aagcaccttt ctgacatatt
      661 taaagtaata aatgaatcag gagtaaaata tgatgtaatt cactttggcg cttgccgagt
      721 aaatagagag gagaaataat catcatttac cataatccct taattgtacg caccactgaa
      781 acgcgctgaa cgcgattctg acgcagacac cacaaaaata gatgttttac cccggaaaaa
      841 caggaaggat gcgtcaggat cgttttcagc tcgctgcata gctatgcatg aaagcgaatg
      901 gagatcactt tgggagctta ccgtgttcat accgtctgtt ttcaacagtt ttctccccgg
      961 gaagctaatc tgccataagc ctggataaca gggcacggtg atactccgta atagcgatca
     1021 gcacctccgc atactccctg tccccgatac gcttgccgtc gataaagagt tttttctgta
     1081 acgcccccag tctcataact tctttcagta cccgatcatc agtcagtgag ttaacgttct
     1141 taccgagagc tgccgcccgt aaaaaaccgg aaacggtctt gccgcattca gcagcccttt
     1201 ttctgatttc ctggtcttct tctgccgtca gacgaacagg gcgactgata gcccgcctgc
     1261 gcgtattact tccacttcgt tttgtcataa aacatggctc cgtatctgac atgggtgtcg
     1321 gggcaa
//
LOCUS       DAJTNM010000269         1313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTNM010000269 DAJTNM010000000
VERSION     DAJTNM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.276"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..277)
                     /locus_tag="OO172_004217"
     CDS             complement(<1..277)
                     /locus_tag="OO172_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5514124.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRK"
     gene            complement(277..876)
                     /locus_tag="OO172_004218"
     CDS             complement(277..876)
                     /locus_tag="OO172_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5514125.1"
                     /translation="MAHIQLVKQTSSGLLLPATSESCEFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYRTPVGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            complement(943..1203)
                     /locus_tag="OO172_004219"
     CDS             complement(943..1203)
                     /locus_tag="OO172_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514126.1"
                     /translation="MKIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHLVDGLVTDGFLLCGDEFVTSLRALKETGLKAGFSAFECE"
BASE COUNT          314 a          362 c          289 g          348 t
ORIGIN      
        1 cctttctcca gatttcttgt gtgcgaaaaa caccttctgc atgcatcagg cgtaattctt
       61 ctttggtgta atcgctggtt tttacccgcc cgtcgattaa atcgtggcat gagctgcagg
      121 caatcgctgc ctgcatatcg tgtggttttg tcgctgttcc gcacgtcccc gccagcctgt
      181 aatgcgccag cacagaagtt tcgggattgt gattgcagta gccaggaatt ctgacggtgc
      241 acatctgccc ccgcgccgct ttacgtaaat ccaccattac gcaaactcca gtaactgtgc
      301 ggccacattt tcgacttcct ccggagagga aaatttacgg aacaggatcc agttccacag
      361 cacattcagt acagatttat aaacctgctg aaactcggtt tcgtccatgt tcgcaaatgc
      421 gatagatttt gccctgcgcc cacggctacc gtccggataa atatgctcgg tgtaaaatcc
      481 ggcctgaatg gttacccact cgcggaaagc ctcaaacgac tttagcaatg ccgtatcccg
      541 ggttctgcgt gtcgcaacgg tattcagata ttgctctgcg gcttcgctca gagctggcgt
      601 atgttcccga cctactgatt cgcacaggta ctcaacgaag cctgatacca gttctcgttc
      661 tcgaggcgtg atcgccccac cgaccggagt ccggtaatcg aatcccagtt gcaggagttt
      721 gaaaaaacgc ttgtggaatg cgtagttacg aacgcgctta aaatcagcgt gtatccactc
      781 gcctgttttg atttgatgca gaaattcgca actctccgac gtcgccggga gaagtaatcc
      841 ggaagaggtt tgtttgacca gttgtatatg tgccatcggc tttctccggt ggcacggtgt
      901 tactcagcag ggggtcaacc ctgcactgaa ttgtagatga actcactcac attcaaaagc
      961 agaaaagcca gccttaagtc cagtctcttt cagcgcccgt aatgacgtga caaattcatc
     1021 tccacacaaa agaaaaccat ccgtcacaag cccatccacc aaatgaatta acgcagctcc
     1081 actcttcctt tgttgagact gtaaacattt aatacggcag tggctgacaa tagcgccatt
     1141 ctcaacgcgc acagtataga ggccatcttc actaaaaatt tcacgtaatt cttcgatttt
     1201 catcaacaga atccttccag gtaaatagca ctcccctgtt cgggggccat ccctcttatc
     1261 cctgcgcgct acttaagtgt ttttgattct attcctgcgc cttctggaag ttc
//
LOCUS       DAJTNM010000270         1312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTNM010000270 DAJTNM010000000
VERSION     DAJTNM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.277"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            137..1057
                     /locus_tag="OO172_004220"
     CDS             137..1057
                     /locus_tag="OO172_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5514127.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1057..>1312
                     /locus_tag="OO172_004221"
     CDS             1057..>1312
                     /locus_tag="OO172_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5514128.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPT"
BASE COUNT          375 a          299 c          312 g          326 t
ORIGIN      
        1 cagtatctcc atacaatcta ttaatcattt aaatgatagg tctaatcatg atttagggtc
       61 taatcaactt aatgatcaat atctatagtg atatcaccaa ccgtcaaaac gaaccgacaa
      121 ctccatgagg aactgaatga tgaataataa agtcagcttc actaacagca ataatccaac
      181 catctctttg tctgcagtga tctatttccc tccaaaattt gatgaaaccc gtcagtatcc
      241 ggcaattgtg gtatctcacc caggtggggg cgttaaagag caaacggccg gaacctatgc
      301 cgaaaagctg gcggaaaagg gatttgtcac tattgcttat gacgcatctt atcaaggtga
      361 aagtggcggc gagccgcgtc agttagaaaa cccatacatt cgtactgaag acgtcagtgc
      421 cgtgattgat tacctgacaa cgctttctta tgtcgacaat acccgaattg gtgcgatggg
      481 aatttgtgcc ggtgcaggat atacagccaa tgctgcaatt caggatcgcc gcatcaaggc
      541 catcggtacc gttagtgcgg tcaatattgg ttcgatgttc cgcaatggtt gggaaaacaa
      601 tgtgaaatct attgatgctc tgccacacat tgaagctggc tcaaacgccc gaaccagcga
      661 tattagcagc gaagaatacg ccattatgcc actggctcca atgaaggagg ccgatgcgcc
      721 gaacgaagaa ctgcgtcagg cctgggagta ttaccacaca cctcgtgcgc agtatccaac
      781 agcaccgggt tacgctactt tgcgcagcct taaccagatt attacctatg atgcttacca
      841 tatggcggaa gtgtacctga ctcagccgat gcagattgtg gcgggcagcc aggcagggag
      901 caaatggatg agtgacgacc tgtacgatcg ggcatcaagc caggataaac gctatcacat
      961 tgttgaaggc gcaaaccata tggatcttta tgacggcaaa gcctatgtgg ctgaagcaat
     1021 ttcagtatta gctccgttct ttgaggaaac tctgtaatga aacatattca aatccgcaat
     1081 agtgatatgg cctggcatat tgctgccaat attcagtttc caccaaattt tgatgagtca
     1141 aaacagtatc cggcgattat cagcgtccat ccttttggca gttgtaagga gcagacgtct
     1201 ggcaatatct atggcaaggc attggctgaa aaaggctatg tcgttctcgc ttatgacgcc
     1261 agtttccagg gagaatctgg tggggagcct cgttggatag aagatccgac ac
//
LOCUS       DAJTNM010000271         1283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTNM010000271 DAJTNM010000000
VERSION     DAJTNM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.278"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..582)
                     /locus_tag="OO172_004222"
     CDS             complement(<1..582)
                     /locus_tag="OO172_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR5514129.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMD"
     gene            952..>1283
                     /locus_tag="OO172_004223"
     CDS             952..>1283
                     /locus_tag="OO172_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514130.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGDIYDESYAPLG
                     LYIENGQR"
BASE COUNT          335 a          328 c          319 g          301 t
ORIGIN      
        1 atccatcaga cacaggcgaa ctttccagcc gatatcttcc agttccgcca gcgtggagag
       61 cgcctcgtcg tactcatcgc cctgatgccc ttccataatc acccggctca actgctccag
      121 gtcgctatag atattttcaa tttcatctgc caactgttcg attttggttt cgaacagatc
      181 cagcagcaac tcgtaggcgt taccgtctac catcgactgg ctacgggcac gcatacgata
      241 cagacgaaaa gcgggcagtt cacgctcacg cagagtaaac agacgaccat cacggatggt
      301 aaatgccaca gtggagttac cggcgtgatc ttccgcatct tcaaagaaga agaaggagtg
      361 aatatgcagg ccgtcgtcgt cttcaaagaa acgtgccgat gcttcgatgt cttccagttc
      421 cgggcgggtt gccaggctct ggccaagttc agattgtacg cgcagtcgct cgtcgtcgtc
      481 cggttcgaca agatcaatcc atactgcatt tacaaggggt tgtgactctt cgacttccag
      541 ccgggtcagt cggttatttt ccagttgaaa tgcgctcagc atgaccggga ctcccaatgc
      601 ttaaaatatc ggacagttcg gtgggcacac agaaacaaat tgggtttcag accattaaac
      661 agcctgactc agcgcgacgg gaaaaattga ggtcgctgac aaccgctaag gctatcagca
      721 aaaagggata gccttaggag ttgatcctgg atgacaggat agtgagccag tatctactgg
      781 gtgtgtccaa ggcgaatgtc ctcttagagt gatcgtgcgc gcatgttacg ccagcaaaat
      841 tttgccgtca acacgcaaca gaacaccaca gaacaatagt tgaacttcgc gctttttggt
      901 actcgtcaag gtcgctaaca ggataaaaaa atcccacatc caggacctat aatggcgtat
      961 cggctactca ttggtaaagg aatgatcacc ttgaatctca aacggatttt tctcaccctc
     1021 accttacttc ccttgtttgc tgttgccgct gatgattgcg cactctcaga tccgacgctg
     1081 accgtacagg cgtataccgt taatcctcaa acagagcggg tgaaaatgta ctggcaaaaa
     1141 gccaatggcg aagcgtgggg aacgttacat gctctgctgg cggatattaa tagtcagggt
     1201 caggtgcaga tggcgatgaa cggcgacatc tatgatgaaa gctatgcgcc gctcggtttg
     1261 tacatcgaaa acggtcagcg gaa
//
LOCUS       DAJTNM010000272         1278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTNM010000272 DAJTNM010000000
VERSION     DAJTNM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.279"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..588)
                     /locus_tag="OO172_004224"
     CDS             complement(<1..588)
                     /locus_tag="OO172_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5514131.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPP"
     gene            complement(1189..>1278)
                     /locus_tag="OO172_004225"
     CDS             complement(1189..>1278)
                     /locus_tag="OO172_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5514132.1"
                     /translation="RTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          336 a          207 c          313 g          422 t
ORIGIN      
        1 aggggggagt tcactaagaa gattccctat tgcgctaatc tcttgtaaag aaaatggtaa
       61 aggtggtaaa actgccagcc cattacatga tacatctaat gtttccagta gttctggtaa
      121 aacaggaaga gaacataatt gattatttga gacatgaagc tccttcaaag atatggggag
      181 tgtgggtagt gtgattagtt gattgtggga cgcattcaat aatttaagtc cttgaggcaa
      241 agcaggcagt tcaataagtc tgttatagct gacattaagt tgtgtaaggg acgcaggcaa
      301 tggggagatt aagcttaaat tatttttact tatattaatt gatttaattc ccggggggat
      361 ttcaggtaat gttgtcaggc ctaattcaga caagtccagg ttcgtctctt ggttttgtag
      421 acatgatact agtcgctgaa aagcgatgtc tcgttgttct tcttgtatgc ggttgttttt
      481 ccattcagtc caatgggtta gataattttc atatgcgcgg ctagtgtcga ttgaataagt
      541 ggagaacgaa tttgaaatta atctgtgatt gttatttgtc gggagcataa atatcagggc
      601 gtattcttta tgttgagagg tgttggattc ttttttatct gctttgcaat gttattgatt
      661 cccttcagca gtagacagga acttctggag gaggcggata tatagtttgt gtaagcatat
      721 taactccata tgttatatat tgaagaactt ctgctttaca ctatcctaaa ctggatcggt
      781 ttaatgtaaa aaaacgatac tttttggggg agggggcaat ttttccttaa ggtatgaaaa
      841 aaggagctga agctatataa tagctattaa tgccactggt atcagtgaat catgtatata
      901 aaaaactgtg ttaaggaggc tgtccattag cggtttttta ttcttctgga accgcggcat
      961 ctttgcgtat ctttttgtct tctcaccgtc tggtcggtgt tttgctgaga ctgctaattt
     1021 cctgtctttg ctagtgctgt actggtactg cccaatcatg attggcgggg gcgggcggag
     1081 ttgagaccgc agacacgtcg tatgcaagaa cgtgctgcgg ctggctggtg aacttccgat
     1141 agtgcgagta ttgaatgatt tccagccgtt actgatttta cgtgctaatt agtgaacaaa
     1201 ccactcgtca gcagactccc aggtatcttt cagagtttcc tgaacaaaag ttttagctga
     1261 atctttatcg gcggtgcg
//
LOCUS       DAJTNM010000273         1263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTNM010000273 DAJTNM010000000
VERSION     DAJTNM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.280"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            66..1259
                     /locus_tag="OO172_004226"
     CDS             66..1259
                     /locus_tag="OO172_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5514133.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          377 a          265 c          289 g          332 t
ORIGIN      
        1 aacgagacaa acatagcatt atgtgatcta ctgatttgtg ccaacaattc aatgagatca
       61 ctgctatgcc tgttcataaa gtatgtcaga atttcttccg ggatgcctta gcaccatttc
      121 atcagtaccg acaaaatgcc ctgatggatg ccacaatggc gttaatcaac ggagcatctc
      181 tgacgcagac cagtatcgga cgttttttgc cgggtaatgc acaagttaag aataagatca
      241 aacgtattga tcgcctcatg ggtaatgagg cgctccatcg cgatattcca atgattttca
      301 ggaatatcac ttcaatgctg acacggcagt tatctttatg tgtaatagcc gtagactgga
      361 gtggctatcc ctcccaggag catcatgttc ttcgtgccag ccttctgtgt gacgggcgtt
      421 ccattccgtt attgagtaag gttgttcctt ctgaaaaaca aaataatcca ttgatacaac
      481 atgatttcct tgattccctt gctcaatccc tgccccccga tgccagggtt attattgtca
      541 cggatgctgg atttcagagc gcatggtttc accatattac ttctctgggc tgggatttta
      601 ttggtcggat cagaaataac gttcagtatt gcctcgataa cgcgcctgaa agatggttaa
      661 aagtgtcgga tagccctgaa tgtaaaacac cggaatatat gggggctgga aggctggtta
      721 aagaaaggaa aaaaagcatc cgggggcatt tttacaccta taagaaatcg gcaaaagggc
      781 gaaaaaagaa acgttcgaaa ggtcagagtg gtttaaacaa aacagacaaa gaacagagca
      841 aatcagcaaa agaagcctgg ttgatcttca gcagtacaaa tgacttcaga gcgcgagaaa
      901 tcatcaagtt atacagtcgg cgaatgcaga tagagcaaaa ctttcgtgat gaaaagaacg
      961 ggcgcttcgg atttggtctt cgggccagca aaagtcgttc aacaggaaga attctggttc
     1021 tgagtctgct ggcaacgttg agcactatcg taatgtggtt actcggttat cacgctgaaa
     1081 ataaagggtt acacctgaaa tatcaggcta acagcattaa atcccggcgg gttatctcat
     1141 atctgacgtt agcgaagaat gttcttcgac actcgccact tattttaaga cgaacagtac
     1201 tgagcacagt tctaaaccat ctgtccagaa cctaccgaaa tatggtgctg gtttattagc
     1261 agt
//
LOCUS       DAJTNM010000274         1255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTNM010000274 DAJTNM010000000
VERSION     DAJTNM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.281"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..1238
                     /locus_tag="OO172_004227"
     CDS             <1..1238
                     /locus_tag="OO172_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5514134.1"
                     /translation="PLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINTA
                     FKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQSE
                     IYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGK
                     TKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNV
                     LYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERL
                     AVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDN
                     NIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLN
                     DWPSNQVHELLPWNFSSVK"
BASE COUNT          336 a          302 c          364 g          253 t
ORIGIN      
        1 aaccgctgcc ggcagaactt ccccgggaga cacatcgcct tctgcctgct gaaaccagtt
       61 gcccggcctg tggaggtgtt ctgaaagaaa tgggggaaac aatctcagag caactggata
      121 tcattaatac cgcctttaaa gttatcgaaa ccatacgtcc caaactggcc tgtagccggt
      181 gtgatgtcat cgttcaggca ccacttcccc ctaaaccgat cgaacgcggt tatgccagtg
      241 cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct ttatatcgcc
      301 agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg gtgcgctggg
      361 tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac tatgttctgg
      421 aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg gggaacggaa
      481 agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg ggttcatccc
      541 tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat ccgcagctcc
      601 acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat aacgtactgt
      661 acgaaacggg ccgggtgaag gaagccgggt gcctggccca cgcccgccga aaaatccatg
      721 acgaggatgt gcgccgtccg acagaaatga ctcaggaagc gctcagacgg atagcagagt
      781 tatacgacat agaagcggag atacgtggca gtccggcaga ggaacggctt gcagtcagaa
      841 aagccagaag cgtccagttg atgcagtcgt tgtacgactg gatacagttg cagaggaaaa
      901 cgctgtcgaa acatgcggag atggcgaagg cgttcgacta tatcctgaat cactggaatg
      961 cgctgaacga gttctgtcgt gacggctggg tggaaataga caacaacatc ggtgaaaacg
     1021 cgttacgatc ggtggcggtt ggaagaaaaa attatctctt tttcggctca gacaagggag
     1081 gagaaagtgc ggcgatcatc tacagtctgc tggtcacctg caaacagaac gaagtggagc
     1141 cggaggactg gttgcgcgaa gtgatcgaga agctcaatga ctggccgtcg aaccaagtgc
     1201 atgaactgct gccctggaac ttctcgtctg taaaataatc cttacgctac gtact
//
LOCUS       DAJTNM010000275         1247 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTNM010000275 DAJTNM010000000
VERSION     DAJTNM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.282"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..554
                     /locus_tag="OO172_004228"
     CDS             <1..554
                     /locus_tag="OO172_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514135.1"
                     /translation="STIHTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQ
                     IREGSWRKERRTSYSREFKVRLAKQALQPGAVVARIAREHDINNNLLFKWKSQYEDGL
                     LSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVV
                     KLFDPLTPELLRALIREMKGGIR"
     gene            551..898
                     /gene="tnpB"
                     /locus_tag="OO172_004229"
     CDS             551..898
                     /gene="tnpB"
                     /locus_tag="OO172_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5514136.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            918..>1247
                     /locus_tag="OO172_004230"
     CDS             918..>1247
                     /locus_tag="OO172_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5514137.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIAS"
BASE COUNT          337 a          319 c          334 g          257 t
ORIGIN      
        1 attccacaat acatacgctt tttcagcgat ttcttgcatc cggtatcgca tggccattgc
       61 ccgattcagt ttcattcgct caacttgacg ccatccttta tgccaacaga aagaaggaat
      121 taacagagcc tcaaatcaga gaaggctcat ggcgaaaaga acggcgaacc agctatagcc
      181 gtgaatttaa ggtccgtctg gctaagcagg cgttacagcc tggggctgtt gttgcccgga
      241 tcgccagaga acacgatatc aataataacc tgctgtttaa atggaaaagc cagtacgagg
      301 acggcttact gagcgatgat gacatacagg aatgcatgcc tgtcccggtg gcactgactg
      361 atacgccgga gccgaccaga ccagttacaa atcccttctg gcgtaacaag catgatgagc
      421 gccctgaggg ggctcccgga aacgtcccac ggtgcgagct gcatcttaaa tcaggtgtgg
      481 taaaactgtt tgaccctctc actccggaac tgttacgggc gctaatccgc gaaatgaaag
      541 ggggtatccg atgataacgc tgccgaccgg taccaaaatc tggatcatcg ctggcatcac
      601 agatatgcgt tgtggcttca atggcctggc ttcgaaggtg cagaacacgc tgaaagatga
      661 cccgttctcc gggcatatct tcgtcttccg gggccgcagt ggcaaaatgg tgaaaatact
      721 gtgggccgat cgtgacgggt tatgcctgtt cgccaaacgc ctggaacggg gccgcttcgt
      781 ctggccggtg acccgggaag ggaaagtgca cctgacgcca gctcagttat ccatgctact
      841 ggaggggatc gcgtggcaac atcccaaacg gacagaacgg cctggcatcc ggatataacc
      901 cgtgataaaa caagggaatg aacaatgaac tccccgatga tattgagctg cttaaagcca
      961 tgttgcgtaa gcaacagagt cggcttcgac agtatgcctg tcaggtcgcg ggctatgagc
     1021 aggaaattga acggctgaaa gcgcaactcg acaggttgcg tcgtatgttg ttcggccaga
     1081 gttcagagaa aaagcgtcat aagcttgaaa atcagatccg acaggcagaa aaacgactgt
     1141 cggaactgga aaaccggctg aacacagcca gaaatcttct ggaagatgca tcgtcagtca
     1201 cagattcacc tgacaccagt cccccgtcag aaaacccgat cgccagt
//
LOCUS       DAJTNM010000276         1238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTNM010000276 DAJTNM010000000
VERSION     DAJTNM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.283"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..912)
                     /gene="ospC2"
                     /locus_tag="OO172_004231"
     CDS             complement(<1..912)
                     /gene="ospC2"
                     /locus_tag="OO172_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5514138.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREF"
BASE COUNT          400 a          195 c          205 g          438 t
ORIGIN      
        1 aaactctcgg aatctttgat ctttactctt tcttataaaa tcaataagat gcaacccaat
       61 tccaagcctc atatcttttg tattaaatat tggcacatca gtttttccct gattaccatg
      121 cacatacctg ctaacttcat ccacaacctc tttaaagtta tctaagaaaa atgattggtg
      181 ctcatggtaa aaaacaggtg gaatagcatt atcaaagtgg tcggttaatg tcatatatcc
      241 atatggagag tcatgatcaa tgatatacgc attagcacca aaatccacag tatgatgtgt
      301 tttatttagt gggagtgttt cttgatgatt ggaaatttca accccaaaaa aaacaaaatc
      361 gtggttagct atttcttcaa tatcctcaga gaatgtgttt ttatatgttt gtatattctc
      421 agacttaatt ttatcaaagg acttaatatt aagagtatta tttgacaggt tagcgttact
      481 ttgatgtctg aagttaaaat cagaattaat aataatagag agcaagtgac gttcatccga
      541 agaaagagga tgagtttttt gtattatttc agaaagatag tgtctggaca ccttatcgag
      601 gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc gcttcaatag aaagatttaa
      661 tgatttgaaa ctggtccctt gagagaacat tttactataa ctttgcgctg ctatagactt
      721 ccttaaaaag cttttcacag tatcagcaca gtgatctaaa tgtttttcac ttatgccaga
      781 agaggatgag tttgttacgc atgatatgtt tttttgtaat gcttttgtat ctttgttcgg
      841 atttattttt ccatcaacaa gatcaatatt gttctgaaca ttaatatgat ttactgcttc
      901 aggtattttc ataaaacatc ccccattatg tgcgttttta tctgcagtta tttagaataa
      961 tattatctaa cttaatattt tcttgtatgt attataacga acatatttta atatgttgta
     1021 cgattttcac tctgaaatcc cctgagttta atcacacttc ctttactgaa aggaattatt
     1081 gcaacagtat tatttgtttt tggatatata tcttgcgcct tgttatgtag ttaatgctca
     1141 gaatatgata gataaaatag agacgcaaaa cagggcatgc tcataacatg atgataacat
     1201 aaaaaattag taacaataaa tgaaagaatc atgggcgc
//
LOCUS       DAJTNM010000277         1220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTNM010000277 DAJTNM010000000
VERSION     DAJTNM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.284"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..489)
                     /locus_tag="OO172_004232"
     CDS             complement(<1..489)
                     /locus_tag="OO172_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR5514139.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQL"
     gene            586..>1220
                     /locus_tag="OO172_004233"
     CDS             586..>1220
                     /locus_tag="OO172_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5514140.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT          352 a          246 c          298 g          324 t
ORIGIN      
        1 aagctgctgt agccgctcat catttggcca agggagttca agtgacattt tcacccccat
       61 ttttatttcc tgaagtatca gtgtctcgag ggctgaaaaa cgtaactgtt gtttatctgt
      121 ggtactttgc gacggtggaa gttggttaat tgcaattagt aattccctta acaggggtga
      181 aatacccgtc atgaagcaaa ctgttcccag cgtttcactt tccgcttctg aaaccagtac
      241 ggtttttaaa agtacgttgc ctgtcgcaac aaacttatgc ggcatttgtg gcggcagcca
      301 aagcgcgctg ctggcatgga ctagccagac gctttctcca gcccatacac gaacgactcc
      361 ttcagccggg taaagcaact gggcatagag atgtttatgc cagggaactg tacctccatt
      421 cagatgttcc gaatccagtc cgataatgtg atgccctggt gatgctttgg gttgcagtgt
      481 aattgtcatt tacagtaggt attttgtcag aaatcatcaa ataagccatg ttaaatttta
      541 aatatgacgt atgcaagttt ccggatatat acggtgtaaa gagtgatgat aatttctaaa
      601 aaacagctta ttggggtggt agcgatcggt attcttttga ctggcgttgt cttttttatc
      661 tggagggtaa gcaaaggaag atttatacag acgacggatg atgcttacat tggtggtaat
      721 atcaccacgg tggcaagtaa agtttctggt tatatttctg ctatagaggt gcgcgataat
      781 cagagcgtaa aaaaaggcga tattatcctg cgtctcgatg atcgggatta tcgtgcaaat
      841 gtcgccaggc ttgaggcaaa aataaaaagt agcaaagcca atctcgaagg cattcaagcc
      901 actattacga tgcagcagtc gataattcaa agtgccagtg aaacatggca ggcagtaaaa
      961 catgaggagc aaaagcgcct gcgagatact gaacgttatg aaaaactcgc acaatcagcg
     1021 gcaatatcgc aacaaattat tgataacgcc aggtttgact accagaaggt cgcagcaaag
     1081 gagcgcaaag ccgcgaacga tttcctggtg gagaagcaac ggctagcagt tttatctgct
     1141 caggaagaaa acgtacgtgc gagtatcgaa gaagttcagg ccgctttgac tcaggcacta
     1201 ctggatctgg aatataccct
//
LOCUS       DAJTNM010000278         1211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTNM010000278 DAJTNM010000000
VERSION     DAJTNM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.285"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..399)
                     /gene="ompG"
                     /locus_tag="OO172_004234"
     CDS             complement(<1..399)
                     /gene="ompG"
                     /locus_tag="OO172_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR5514141.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRW"
     gene            complement(444..>1211)
                     /locus_tag="OO172_004235"
     CDS             complement(444..>1211)
                     /locus_tag="OO172_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5514142.1"
                     /translation="IAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAI
                     VREAGVFLMDEPLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVI
                     MKDGIVQQVGAPKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEK
                     LAVLKTQESLHKPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHEL
                     VVRAGALNDYHAGENITIHFDMTKCHFFDAETEIAIR"
BASE COUNT          276 a          347 c          257 g          331 t
ORIGIN      
        1 ccagcgctgc atattcgccg tgtctttgcc cggttcatca acgtagtgat aaccataatt
       61 acggaaaccg ccggtcaggc cgaaactgaa atcatcgctt tcgaggaact gataatgcac
      121 ctccagctcc gggcgatcaa accacgttcc acgtttaccc gcgctataat ctaccggccc
      181 ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa aatagactga
      241 aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta tttcgtacat
      301 cgcaccgata ttaaagtgcc agtcgttctt ttcctcggcc tgtgcgcagg ccattcccgc
      361 acacatcacc agtgcggtac agggtaataa ctttttcatt attgttcctt gtattatcct
      421 aggggaatgc cttccccctg tatttagcga attgctattt ccgtttctgc atcaaagaaa
      481 tgacatttcg tcatatcaaa atgaatagtg atattttctc ctgcatgata atcatttaac
      541 gcaccagcac ggaccactaa ctcgtgcccc ccaaccgtgg tgtagagcat aaattccgca
      601 ccggttaatt ctgccacgct aattttggcg gaaatgttat tttcctcttg cgcgtccgga
      661 tgaatatctt ccgggcgtat tcccatcacg atgggcttat gcaaactttc ctgtgttttc
      721 agaaccgcta atttctcttc gagaatggtt aatttaagcg tttccgtaac gaatttatcg
      781 ccatcgatcg tgccgcgaat aaaattcatc gctggtgatc caataaatcc ggcaacaaac
      841 atattcgcgg gttggttata aacggttttc ggcgcaccta cttgctgaac aatcccgtct
      901 ttcataatca caatccgcgt cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag
      961 atcatggtgg tgttcagttt ctgatgcagc ttgctgatct ctgcacgcat ttgtacgcgc
     1021 agcttggcat caaggttaga gagcggttca tccattaaaa acacgcccgc ttcgcgcaca
     1081 atcgcccgcc cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt
     1141 ttcaggtact cacgcaggcc gagaatttgc gccgcccagt tcacccgctc atcaatcacc
     1201 tctttggcga t
//
LOCUS       DAJTNM010000279         1193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTNM010000279 DAJTNM010000000
VERSION     DAJTNM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.286"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            101..1051
                     /gene="virK"
                     /locus_tag="OO172_004236"
     CDS             101..1051
                     /gene="virK"
                     /locus_tag="OO172_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5514143.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
BASE COUNT          354 a          221 c          247 g          371 t
ORIGIN      
        1 tatcagatgg ctcgcctggc aaaacaggat atagaagagc ggtttgatat taataaaact
       61 gcgttaaaaa tactcacatt agcgaagcaa aagtaacaat atgttttcta taagtaactt
      121 atcatttatc ggtttcctta aaaggattat tttttcctca gattcactac cggggaagtg
      181 ggaacacaga aaatttcggt tcatgtacat tttgcgatgt tctataaatc cggttgtcag
      241 tattcgatat tattatgaac tgcgttcctt gccgtgcatt gaggatattc tggcaataca
      301 ccccacgttg ccagcaagaa ttcatcgccc ttatttgcat aaaggggggc gcgcctggac
      361 ccgtggacaa tatattcttg agcattatcg ctttgtgcaa aatttgccag aaaaatattc
      421 taaatttttg tttccacaaa aatcggtatc attagtccaa ttcatcggga aagatggaga
      481 gaattttgat atccagtgtt ctcccagtgg ttttgaccgg gaaggagagt tgatgttgtc
      541 attgttttac aataaaacag ttattgcccg gttaacattt tcagtcattc tgactcaaaa
      601 tggacacatt gcttttattg gcggattaca gggagcccct aaaaataccg gacctgatgt
      661 tattcggtgc gcaacccggg cctgttatgg gattttccca aaacggatta tttttgaagc
      721 attttgtgcg ttgatgaaag cttgcaatat ctctgagtgt ctggcagtca gtgagcacag
      781 tcatgttttc cggcaattga gatactggta tcagaaacgc aaaacctttg ttgcggtcta
      841 tagtgatttc tgggagtctg tagcaggaaa aacctgtggt gactggtaca gattaccaac
      901 ccaggtgatt cgaaagccgc taagcgatat agccagtaaa aaacgctctg agtaccgaaa
      961 acgatacgct ttgctggatt atatccatga aactactatc cgctctttgg atgcatatcc
     1021 tgtacactca gaacatcaga atttaaatta aggtttgatg tattctctga ataaaacagt
     1081 aatgataatt ttggtagagg cattcgcact aaataatgaa aaaatataaa tctgagttta
     1141 ttcctgaatt taagaaaaat tacctttccc ctgtttactg gtctacatgg ttc
//
LOCUS       DAJTNM010000280         1159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTNM010000280 DAJTNM010000000
VERSION     DAJTNM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.287"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>1159
                     /locus_tag="OO172_004237"
     rRNA            <1..>1159
                     /locus_tag="OO172_004237"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          289 a          267 c          365 g          238 t
ORIGIN      
        1 ggcgcaagcc tgatgcagcc atgccgcgtg tatgaagaag gccttcgggt tgtaaagtac
       61 tttcagcggg gaggaaggga gtaaagttaa tacctttact cattgacgtt acccgcagaa
      121 gaagcaccgg ctaactccgt gccagcagcc gcggtaatac ggagggtgca agcgttaatc
      181 ggaattactg ggcgtaaagc gcacgcaggc ggtttgttaa gtcagatgtg aaatccccgg
      241 gctcaacctg ggaactgcat ctgatactgg caagcttgag tctcgtagag gggggtagaa
      301 ttccaggtgt agcggtgaaa tgcgtagaga tctggaggaa taccggtggc gaaggcggcc
      361 ccctggacga agactgacgc tcaggtgcga aagcgtgggg agcaaacagg attagatacc
      421 ctggtagtcc acgccgtaaa cgatgtcgac ttggaggttg tgcccttgag gcgtggcttc
      481 cggagctaac gcgttaagtc gaccgcctgg ggagtacggc cgcaaggtta aaactcaaat
      541 gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgatg caacgcgaag
      601 aaccttacct ggtcttgaca tccacggaag ttttcagaga tgagaatgtg ccttcgggaa
      661 ccgtgagaca ggtgctgcat ggctgtcgtc agctcgtgtt gtgaaatgtt gggttaagtc
      721 ccgcaacgag cgcaaccctt atcctttgtt gccagcggtc cggccgggaa ctcaaaggag
      781 actgccagtg ataaactgga ggaaggtggg gatgacgtca agtcatcatg gcccttacga
      841 ccagggctac acacgtgcta caatggcgca tacaaagaga agcgacctcg cgagagcaag
      901 cggacctcat aaagtgcgtc gtagtccgga ttggagtctg caactcgact ccatgaagtc
      961 ggaatcgcta gtaatcgtgg atcagaatgc cacggtgaat acgttcccgg gccttgtaca
     1021 caccgcccgt cacaccatgg gagtgggttg caaaagaagt aggtagctta accttcggga
     1081 gggcgcttac cactttgtga ttcatgactg gggtgaagtc gtaacaaggt aaccgtaggg
     1141 gaacctgcgg ttggatcac
//
LOCUS       DAJTNM010000281         1131 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTNM010000281 DAJTNM010000000
VERSION     DAJTNM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.288"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>1131
                     /gene="amn"
                     /locus_tag="OO172_004238"
     CDS             <1..>1131
                     /gene="amn"
                     /locus_tag="OO172_004238"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5514144.1"
                     /translation="NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTIT
                     RPTLFRSYLNEQLTLLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYF
                     PTTELAQIGDETADGIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFT
                     NYTRYVDEFVRWGCSQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPA
                     WHLITADGQGITLVNIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVL
                     AHAYLRDDHVLDAVLPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTT
                     DDRNWELRYSASALRFNLSRAVAIDMESATIAAQGYRFRVSYG"
BASE COUNT          268 a          301 c          266 g          296 t
ORIGIN      
        1 aacgcccgta aacaaggtct ttttgtctat ccatcactga ccgtaacctg ggacggtagc
       61 acaacaaatc cccccaaaac gcgcgcattt ggtcgtttta ctcacgcagg cagctacacc
      121 accacgatta ctcgccctac tctctttcgt tcgtatctga atgagcaact aacgttgctt
      181 tatcaggatt atggtgcgca tatctctgtg caaccctcgc aacatgaaat cccttatcct
      241 tatgtcatcg atggctctga attgacactt gatcgctcaa tgagcgctga tttaactcgc
      301 tacttcccga caacagaact ggcgcaaatc ggcgatgaaa ctgcagacgg catttatcat
      361 ccaacggaat tctccccgct atcgcatttt gatgcgcgcc gcgtcgattt ttccctcgca
      421 cggttgcgcc attataccgg tacgccagta gaacattttc aaccgttcgt cttgtttacc
      481 aactacacac gttatgtgga tgaattcgtt cgttggggat gcagccagat cctcgatcct
      541 gatagtccct acattgccct ttcttgtgct ggcgggaact ggatcactgc cgaaaccgaa
      601 gcgccagaag aagccatttc cgaccttgca tggaaaaaac accagatgcc agcatggcat
      661 ttaattaccg ctgatggtca gggtattact ctggtgaata ttggcgtggg accgtccaat
      721 gctaaaacca tctgcgatca tctggcagtg ctacgcccgg atgtctggtt gatgattggt
      781 cactgtggcg gattacgtga aagccaggcc attggagatt atgtccttgc acacgcttat
      841 ttacgcgatg accacgttct tgatgcggtt ctgccgcccg atatacctat tccgagcatt
      901 gctgaagtgc aacgtgcgct ttatgacgct accaagttag tcagcggcag gcctggtgag
      961 gaagtcaaac agcggctgcg tacaggtact gtggtaacca cagatgacag gaactgggag
     1021 ttacgttact cagcttctgc acttcgtttt aacttaagcc gggccgtagc aattgatatg
     1081 gaaagtgcaa ccattgccgc gcaaggatat cgtttccgcg tgtcatacgg g
//
LOCUS       DAJTNM010000282         1107 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTNM010000282 DAJTNM010000000
VERSION     DAJTNM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.289"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..387)
                     /locus_tag="OO172_004239"
     CDS             complement(<1..387)
                     /locus_tag="OO172_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR5514145.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQVDLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            816..1097
                     /locus_tag="OO172_004240"
     CDS             816..1097
                     /locus_tag="OO172_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5514146.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          308 a          249 c          251 g          299 t
ORIGIN      
        1 aacgttaggt tcacgcttat aatcggccgg tgcaatcgta tagccgttta caggcgctat
       61 cagcgatgag cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata
      121 gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata
      181 gcgttcagct gcatcgctca ctaaatctac ctggcgaatg accagctcgg atttatccag
      241 tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc
      301 aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag
      361 taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc
      421 attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca
      481 cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt
      541 gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg
      601 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
      661 acaatcaatt gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
      721 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
      781 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
      841 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
      901 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
      961 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1021 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1081 caatgatttt gacctgatac gtagaag
//
LOCUS       DAJTNM010000283         1089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTNM010000283 DAJTNM010000000
VERSION     DAJTNM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.290"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..122)
                     /locus_tag="OO172_004241"
     CDS             complement(<1..122)
                     /locus_tag="OO172_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5514147.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHL"
     gene            complement(194..1078)
                     /locus_tag="OO172_004242"
     CDS             complement(194..1078)
                     /locus_tag="OO172_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCR5514148.1"
                     /translation="MVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDD
                     PFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETT
                     DLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLL
                     RLCLRQALQLADVVKISEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVC
                     YRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGAL
                     AVTAKGAMTALPCRQELE"
BASE COUNT          291 a          313 c          229 g          256 t
ORIGIN      
        1 agatgtcctt tcagccaaat agcgtataac gaccaccaca gcgaccagga aataaaaaag
       61 agaaatgagt aactggatgc aaaacgatag tacgcatttc tgaatggaat attcagtgcc
      121 ataattacct gcctgtcgtt aaaaaattca cgtcctattt agagataaga gcgacttcgc
      181 cgtttacttc tcactattcc agttcttgtc gacatggcag cgctgtcatt gcccctttcg
      241 ccgttactgc aagcgctccg caacgttgag cgagatcgat aattcgtcgc atttctctct
      301 catctgtaga taatcccgta gaggacagac ctgtgagtaa cccggcaacg aacgcatctc
      361 ccgcccccgt gctatcgaca caattcacag acattccagc aaaatggtga acttgtcctc
      421 gataacagac caccacccct tctgcacctt tagtcaccaa cagcatggcg atctcatact
      481 cttttgccag ggcgcatata tcctgatcgt tctgtgtttt tccactgata agtcgccatt
      541 cttcttccga gatcttgacg acatccgcca gttgtagcgc ctgccgcaaa cacaagcgga
      601 gcaaatgctc gtcttgccat agatcttcac gaatattagg atcgaagctg acaaaacctc
      661 cggcatgccg gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc tcggcagaca
      721 acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag tctgtcgtct
      781 ctaaaaaaag atcggcactg gggcggacca taaacgtaaa tgaacgttcc ccttgatcgt
      841 tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga tacgtgatat
      901 cgactccctc agttagcagc gttctttgca ttaacgcccc aaaaggatca tcaccgaccc
      961 gacctataaa cccacttatt ccgcctaatc tggcgattcc caccgcaacg ttagctggcg
     1021 cgccgccagg acaaggcagt aggcgcccgt ctgattctgg caagagatct acgaccacat
     1081 cccctaaaa
//
LOCUS       DAJTNM010000284         1076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTNM010000284 DAJTNM010000000
VERSION     DAJTNM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.291"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..>1076
                     /locus_tag="OO172_004243"
     CDS             1..>1076
                     /locus_tag="OO172_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5514149.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLASRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSR"
BASE COUNT          244 a          277 c          342 g          213 t
ORIGIN      
        1 atgttgtccg cttttactcc ccgcccgctg aaacgcctgt tcacagccaa ccagtgctgg
       61 acgtccttcc tggatgcggg cggtctgcgc gatatcgagg ttgaggctgt caccaaaatg
      121 ctggcctgcg gcacgcggat actgggcgtg aaggaataca tctgcgataa acctgagtgc
      181 ccccacgtca gatacgtcac taactcatgc ggcagccgtg cctgcccgtc ctgcggaaaa
      241 aaggccacag acctgtggat agcgacacag ctgaatcgtc ttcctgactg cgactgggta
      301 cacctggtct tcaccctgcc ggacacgctg tggccggtgt tcgaaagcaa ccggtggctg
      361 ctgaatgacg tgtgccgtct ggcggtggag aatctgctgt atgccgcccg gaaacggggg
      421 caggaacccg gtatcttctg cgccatccac acgtatggcc gtcgtctcaa ctggcatccg
      481 catgtacatg tgtctgtaac ctgtggaggt ctgaataagc atggtcagtg gaaaaagctg
      541 agcttcctga aagacgcgat gcgttcgcgg tggatgtgga atatgcggca acggcttctg
      601 aaagcgtggt cagaggggct ggcgatgccg gagtcgttgt cacatatcac gacggaatca
      661 cagaggagaa gcctggtgct gaaagccgga ggaaaatact ggcatgtgta catgtcgaaa
      721 aaaacggccg gcgggaggaa tacggcgcgc tacctgggtc gttatctgaa gaagccgccg
      781 atagcggcct cccggctggc acattacaac ggaggggcga gcctgagctt ccgttacctg
      841 gaccacaaaa cgggagaaac ggcgacggaa acgctgacac agcgtgagct ggtcgcgagg
      901 ctgaaacagc acatcccgga gaagtttttt aagatggtga ggtacttcgg gttccttgcc
      961 agccgggtgt gtggagagaa gctgccgcag gtgtaccgtg ctctggggat ggataaaccg
     1021 gaaccagtgg cgaaagtgtg ctatgcacaa atggtgaaac agttcctgag tcgtga
//
LOCUS       DAJTNM010000285         1048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTNM010000285 DAJTNM010000000
VERSION     DAJTNM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.292"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>1048
                     /locus_tag="OO172_004244"
     CDS             <1..>1048
                     /locus_tag="OO172_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5514150.1"
                     /translation="PLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKT
                     AQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQ
                     MELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEH
                     RHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKV
                     CHLLTSMTDAMRFPGGEMADLYSNRWEIELGYREIKQTMQLSRLTLRSKKPELVEQEL
                     WGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMR
                     DLASMGQLVKLPTRR"
BASE COUNT          279 a          265 c          307 g          197 t
ORIGIN      
        1 cctcttcacc agattgtgaa tcgcctggac atcatgctgc cgggcaatcg ccccttcgtt
       61 gcccccagtg ccgttattca ggcccgccag cgcctgggaa gtgaggctgt ccgccgcgtg
      121 ttcacgaaaa cagcgcagct ctggcataac gccacgccgc atccgcactg gtgcggcctg
      181 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
      241 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      301 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      361 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      421 atggataaag gttattactc actgggactg ttaaatggct ggagcctggc gggagaacac
      481 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      541 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      601 ctgggaaatg aagtgacagc ccgcctgctg accgtgacgc gcaaaggaaa agtctgccat
      661 ctgctgacgt cgatgacgga cgccatgcgc ttccccggag gagaaatggc ggatctgtac
      721 agtaatcgct gggaaatcga actgggatac agggagataa aacagacgat gcaactgagc
      781 aggctgacgc tgagaagtaa aaagccggag cttgtggagc aagagctgtg gggtgtctta
      841 ctggcttata atctggtgag atatcagatg attaaaatgg cggaacatct gaaaggttac
      901 tggccgaatc aactgagttt ctcagaatca tgcggaatgg tgatgagaat gctgatgaca
      961 ttgcagggcg cttcaccggg acgtataccg gagctgatgc gcgatcttgc aagtatggga
     1021 caacttgtga aattaccgac gagaaggg
//
LOCUS       DAJTNM010000286         1018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTNM010000286 DAJTNM010000000
VERSION     DAJTNM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.293"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(117..584)
                     /locus_tag="OO172_004245"
     CDS             complement(117..584)
                     /locus_tag="OO172_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5514151.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(650..889)
                     /locus_tag="OO172_004246"
     CDS             complement(650..889)
                     /locus_tag="OO172_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5514152.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
BASE COUNT          349 a          218 c          185 g          266 t
ORIGIN      
        1 agaacctcgc ttaggcctgt gtccatatta cgtgggtagg atcacaagac ctactgctat
       61 cacaattact gagagaaaaa aagcatccga tgattcattt atgctgtcct aaaaaattaa
      121 caaaacaatg ggaaaacagg atatgtaagc agaaaagcca atgaaattgg tacagcataa
      181 ggcacaccgt tttcacgaat tgattttcga aaaaaaagaa gacctgccag aacaaccaga
      241 gcgccgcaaa aggtcattgc gacgagaaaa ttagccagtt catgttcatc aatagctaac
      301 gaaagaacag ttaacagctt gacatcaccg ccgccaatta tccctgcgga aaaaagaaag
      361 aagcccacga gcaaaacgct cccggctgca accagattgg gtttttgatg ctgtaaaatg
      421 agtgccaaaa aaataagcac ggttaacacc agtaaataac gatggctaat aattctgagt
      481 aatatatctc cccatgaaat aaaaataaga aagagcgcaa caaataaaag atcgactctt
      541 agtaaccata ttgctgccat tgaaagactc catgtaccat acaaggttgc tgccagacat
      601 attcctttac gtctgacagc ctctagagag tttttttatt ttttataagt tacggattac
      661 tcgttccgga aatcgttacc tgttgaattg ctgcagcgat agcgtcgaat gcatctttaa
      721 ttgctcccag gaagcctgag ttctgatcgc caaagatgaa agcaagcaga gttgccatag
      781 caacaccaat caatgcatat tcgattgcag taacaccacg ttcatccttc gcgaaacgcg
      841 caaagcactg tttagtttta ccatagtact tagtaataaa agtattcatg ttttcctcgt
      901 atattattat ttaaattcaa accaccagct tcatctgcgt gtcaccggta tcccatattt
      961 ttgggatgtg gctataatta tccattcccg ctatttagtc ttcaaaaaaa attacatt
//
LOCUS       DAJTNM010000287          988 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTNM010000287 DAJTNM010000000
VERSION     DAJTNM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.294"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(32..201)
                     /locus_tag="OO172_004247"
                     /pseudo
     CDS             complement(32..201)
                     /locus_tag="OO172_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            290..562
                     /locus_tag="OO172_004248"
     CDS             290..562
                     /locus_tag="OO172_004248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514153.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASGSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
BASE COUNT          242 a          193 c          240 g          313 t
ORIGIN      
        1 catcacacca ctgtgctaat gaaaaatgta atcagtgtgg tggacgtgtt ttatccgcat
       61 tggaattgtc gtcaactaaa ggagatgagg cccgtttgta tcgcaatggg ttatgcccgt
      121 ttgctgttcg ataatggctt ctctgagcat acggtgttgc agttctcgta aaaactttgc
      181 gtcttctggt gataaatcca ttgttattac tcgtaaagca atgcgctgta gtgccttacg
      241 ctaccgcgct gatggttctt atccgtttta ttgaatcgga gaacgaactg tgagctatca
      301 gcctggtggt ctggttttgc ctgatttaag ccgccgtggg tatttgtcca agaatgcttc
      361 aggctccaat cgttttggcc tggtgaatgg agatgggtta agcaaagact ctcgttgctg
      421 gtgtactttt tcgcgctggc gctcaagcat ggataatgcg attgcctgta gttcttcgtt
      481 agtcattgtc tgcacctgga aagcaattta ccggagtacc tcacactacg gcaaaaatgt
      541 tttattgttg caacttgcgt aattattcag gtcagtttgg aacgcactgt ataaacataa
      601 ataacctgat actgttgtgt gttttcaaag aaaacacagg tatttttcac ataaaccttc
      661 cccttttcat attcgaccac ttaatcaaaa aacggtttta tacttgttgc ccccactggg
      721 gttaaatgtg agacatgtcg cactttgtgc catgtgtcct tgctgatttt aggcgcggtt
      781 cattcattct ttctgttggc ataaagtatg gtatcggttg ggcgaatgaa agggactcgg
      841 gtcatggtca gacaatacct gaggcgttaa ttcgttgaaa gagattgtga atgggggaat
      901 aactgagtct taactgctgt gcgatagccc ggacagataa ttgctcgtcg aatctgagac
      961 gtaatgattc tggcaaataa gtgcgaaa
//
LOCUS       DAJTNM010000288          986 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTNM010000288 DAJTNM010000000
VERSION     DAJTNM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.295"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(23..826)
                     /gene="dkgB"
                     /locus_tag="OO172_004249"
     CDS             complement(23..826)
                     /gene="dkgB"
                     /locus_tag="OO172_004249"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5514154.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLHTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          221 c          222 g          284 t
ORIGIN      
        1 ccggaggagc tgtcagagag gcttaatccc attcaggagc cagaccttcc gggctaacca
       61 ggcggtcgtt gcaatccagt gcggcgatcg cttttttatc ttcggcatca agctgtaaat
      121 tttgtgcctt aagattactt tccaggtttt tacgtttagt agaagaagga attactgagt
      181 aaccttcccc catagcccac gccagaatca cttgtgccgg aatcgcattg tgtttagctg
      241 caatacgagc aataacctca tctttcagtg ccttaccata agccagcgtc atataggaag
      301 tgatatgaat accgtgctgt ttagcccagg caaccacttt acggttttgc agataaggag
      361 agagttcaat ctggttggta gcgatgtttt cagcgccaac agcagcaatc gctttttcca
      421 tcaatgggat cgtgaagttg gaaataccga tctcacgcgt cagcccttgt tttttggctt
      481 ccagcagcgc cttcataaac tcttcaacag agacttcatc gtttggtgac ggccagtgga
      541 ttagcgtcag atcaacataa tcggtatgca atttttgcag gctctctttc agactcggga
      601 tcaatttgtc tttgctgaga ttttcaatcc agattttagt ggtgatgtag agttcatgac
      661 gtggcacgcc actttctgca atcgcctgac ctactgcggc ttcgttatca tagatttgtg
      721 cggtatcaat tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa ataacaacgt
      781 cgtctttcag acggaaagta cctaaaccaa atgcagggat agccataata tgcctctttt
      841 aatgctattt tcgttaactg aggtgattat gggcttcaga ctgttgcgga aaaagagcgt
      901 aaaacgcaga ggatttttgc gattctagca ataatagata tccagacgca aaaaaacatc
      961 gagcgttaac tcgaggcttc ttaata
//
LOCUS       DAJTNM010000289          961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTNM010000289 DAJTNM010000000
VERSION     DAJTNM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.296"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..853
                     /locus_tag="OO172_004250"
                     /pseudo
     CDS             1..853
                     /locus_tag="OO172_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692680.1"
                     /note="The RecE (also known as exonuclease VIII) pathway
                     is an alternative pathway for the initiation of homologous
                     recombination in Escherichia coli. The RecE pathway is
                     activated in recB, recC sbcA (suppressors of recB and
                     recC) mutants. Activation of the RecE pathway results in
                     the production of at least two phage proteins relevant for
                     recombinational repair. RecE is a duplex DNA-specific
                     exonuclease which produces 3' overhangs in duplex DNA by
                     preferentially degrading the 5'-ending strands.
                     Recombinational repair of E.coli chromosomal DNA involves
                     as well other proteins: RecA, RecF, RecJ, RecO, RecR, and
                     RuvC.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
BASE COUNT          291 a          250 c          230 g          190 t
ORIGIN      
        1 tatcacgaaa aaacatcagc cctatgaatt taagaatttc atttcagcct ggctggatac
       61 cgaacacatt gatcgcggtc ttctgacaaa agaatggcga aaagggaatc gtgtttcacg
      121 catcactcgc acgacttccg gtgctaatgc tggcggcggg aacctcaccg atcgcggcga
      181 aggtttcgtc cacgatctga cgtcactgac gcgcgatgta gccactggcg tactggcccg
      241 ttcaatggac gtggacatct ataaccttca tccggcacac gctaaacgca ttgaggaaat
      301 tatcgctgaa aataaaccac ccttttctgt tttccgcgac aaattcatca ccatgcctgg
      361 cgggctggat tattcccgcg ccatcgtggt tgcgtccgtg aaagaagcac caattgggat
      421 cgaggtcatc cccgcacacg tcactgaata tctgaacaaa gtactgactg aaaccgatca
      481 tgccaaccct gatccggaaa tcgtggatat tgcctgcggt cgctcctctg ccccgatgcc
      541 gcagcgagta acagaagaag gaaaacagga cgatgaagaa aaaccacaac catctggcgc
      601 aatgacagat gaacaggcaa cggctgaaac agtggaaccg aatgcaactg aacatcatca
      661 gaacacgcag ccgctggatg ctcagtcaca ggtaaattct gttgatgaga aatatcagga
      721 actgcgggca gaactccatg aagcccggaa aaacattccg cccaaaaatc ctgtcgatgc
      781 agacaaatta ctggctgcct ctcgcggaga atttgttgaa gggattagcg acccgaatga
      841 ttcgaaatgg attcccggtc atcatatctc ctcaaatgaa gtcaaaaaaa cggaaaatga
      901 agtgcgacag actgaagata aacagcacca gaacagcgaa ccggaggagg gaacaccctg
      961 c
//
LOCUS       DAJTNM010000290          959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTNM010000290 DAJTNM010000000
VERSION     DAJTNM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.297"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            79..>959
                     /locus_tag="OO172_004251"
     CDS             79..>959
                     /locus_tag="OO172_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR5514155.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGGIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKAQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTMYNDVL"
BASE COUNT          256 a          227 c          239 g          237 t
ORIGIN      
        1 ctgtgcgctg ttgtccatgt gatattgtgt accagatgtg tgccaaacta ttatttctga
       61 gcgtaccaaa tattatttat ggctataagc gacacaaaac tgcgtactat ttatggtaaa
      121 ccatattcgg gcccacaaga agtggctgat gccgatggcc tcagcgtacg aatttcaccg
      181 aaggggggca tccagttcca gtaccgctat cgctggcatg gcaagcctaa tcgacttggg
      241 cttggtcgat acccatccct gtctttgaag gatgccagac agatcactgc tgacttgcga
      301 aagctctatt tctcaggaac ggatccacgc acctattttg aagagaaggt ggagaactcc
      361 atgacggtcg cccagtgtct cgactactgg ttcgacaact acgtctctac aactctcaga
      421 gaaaaggccc aggcacttta tcgatcaacg gttatgaagc gcatgcatga cgccttccct
      481 aatcgtccgg catcttctat cacggttaag caatgggttg acctgcttac cgaagaagaa
      541 aaagataatc cacgccgagc aaggcaggtg ctaagtcaac taagatcagc aattagttgg
      601 tgcatgcggc gtcagttgat agatagttgc gcaattatga gcatccagcc aagggacttc
      661 ggctcccgcg ctgaggtagg ggatcgggta ctgtcgtatc acgaactggc taagatttgg
      721 cttgctattg aaagaagccg tgcgtctacg tcaaataagc tccttcatca gatgcttatg
      781 ctgtgggggg cgaggctctc agagcttagg ctggcaacaa agacagaatt tgacctgctg
      841 gacaacgtat ggaccgtacc gaaagagcat agcaagatgg gtaatgttat ccgccgtcca
      901 atcttcgaac aaattaagcc ttttctcgaa aaggccatga caatgtacaa tgatgttct
//
LOCUS       DAJTNM010000291          941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTNM010000291 DAJTNM010000000
VERSION     DAJTNM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.298"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(201..>941)
                     /locus_tag="OO172_004252"
     CDS             complement(201..>941)
                     /locus_tag="OO172_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR5514156.1"
                     /translation="LLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPL
                     YVSLYPTEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFR
                     PREVQGTMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTI
                     LEIRPRKRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALS
                     RSETVLQKSLEITEEADLALRSAMARIK"
BASE COUNT          235 a          265 c          214 g          227 t
ORIGIN      
        1 aacaccatca tacactaaat cagtaagttg gcagcatcac ctaagtgctt ttacgatgaa
       61 tttcatgata actctcctta tgacattaat aaatgcgtta atcttaaatc tcacccaggt
      121 aatatatatc tgaaaatatt ttcaacaaca ccagacagga aaaacaccac cacaggcggt
      181 ggcgtttctt tggggcgtct ttatttaatc cgggccatcg cgcttctcag tgccaaatct
      241 gcctcttccg ttatctccag acttttctgc aacaccgttt ctgaccggga cagtgcctga
      301 cgtgctgcca gcggtttccg gatatgctcc atcgtcagca tcgtgtcctc gtagccctgc
      361 tgacgccagg cgtcggtatg tgccgacgtg aaactgagca gaccgccact gttgccacca
      421 tcacctgcat atttcagcct tttccgggga cggatctcaa ggattgtggt gtccggaaaa
      481 gcctggcgat cccataaaga accgtcactc agatgcgaca caatcaccat attgcatccg
      541 gcatcgacca gaggcgttac aggggtattt ccctgcatat ttcgccatcc tcccatacca
      601 ccatcaccga acattgtccc ctgaacctca cggggacgga acaacagggg cagtgcggct
      661 gacgcaagca gagcctcttt ctgctgtccg cggggcagac tctggatatg ctgaaaaacg
      721 gcgtttttcg tggttccgga acccagttca gcacgaatgc agtcaataat atcctgcatg
      781 cccccttctg tggggtacag cgacacatac agcggtagcc cgcctgccag agcatctgta
      841 tcaagataat gatccatcaa cgctgtcagg ggcccatctg acaacaacgg ctgagtcatc
      901 ggattgtcga agccgttgat tatcgatata tggcgaagaa g
//
LOCUS       DAJTNM010000292          932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTNM010000292 DAJTNM010000000
VERSION     DAJTNM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.299"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..119)
                     /locus_tag="OO172_004253"
     CDS             complement(<1..119)
                     /locus_tag="OO172_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5514157.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
     gene            complement(430..534)
                     /locus_tag="OO172_004254"
     CDS             complement(430..534)
                     /locus_tag="OO172_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5514158.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            complement(809..>932)
                     /locus_tag="OO172_004255"
     CDS             complement(809..>932)
                     /locus_tag="OO172_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5514159.1"
                     /translation="VEHSHYLRIYMGHLRQKLEQDPARPRHFITETGIGYRFML"
BASE COUNT          303 a          172 c          183 g          274 t
ORIGIN      
        1 actgataaaa taatggcggc aacgtcaata taattacttt cgtcaatatt cacgcatgca
       61 cgatgagtgg aaaaacaatc cgggcaagaa cggctaacgg caatttttaa ttctgacatt
      121 ttattcatct ctttatttca ggtaatagca ggtcctcaat ttctcaaagg agaaatcgat
      181 cacgccggaa aaaaagcaaa gctccaccgc tgataaataa atcagtagtt tgctttttgc
      241 agatgcaata acccagacca gcccggtaac tggctatact cgtcatacct caagttgcaa
      301 gtgctgcgac tcgaattatt tagagtttaa aagggttagc agtatatgag caccgaggaa
      361 tacaggtatt aacctgtaat gcttaaagtc agggagacct gtacgttggg gtgcccttta
      421 gtcagaagac tagcgggcat taaagaaatg aaagtcaaaa ctattacgat gggcaaactt
      481 catgatatgg gttgtccgcc ttatatgagg cattaagcgg ttattatttt ccatttacct
      541 ttaccgcttg ctgtgtcagt tgaaggtaga gttattctat ccatgaaatg gctataatct
      601 gtgaagaaga tcaacaaaaa acgatcatac cccagataat aaatcattaa ttatgaatat
      661 cgccatatta gatgctgttt tgttacttaa atgttgtaat ttcgtgtttt atatttgcgt
      721 taagaaatta aacctgccaa ataataatat tcggcgcagg tttaataata aattaatcac
      781 tatttaagcg aatttattga ataacaattc aaagcataaa ccgataccca ataccggttt
      841 cagtaatgaa gtggcgaggc cgggccggat cctgttccag tttttgtcgc agatgtccca
      901 tataaatacg caaatagtga ctgtgttcga cc
//
LOCUS       DAJTNM010000293          924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTNM010000293 DAJTNM010000000
VERSION     DAJTNM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.300"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..765
                     /locus_tag="OO172_004256"
                     /pseudo
     CDS             1..765
                     /locus_tag="OO172_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(774..>924)
                     /locus_tag="OO172_004257"
     CDS             complement(774..>924)
                     /locus_tag="OO172_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514160.1"
                     /translation="GLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRASNGLSDN
                     RCLEI"
BASE COUNT          244 a          205 c          219 g          256 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcgctga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagcag gttggatttg cccctatatt
      781 tccagacacc tgttatcact taacccatta ctggcccgct gccgtagata ttcccgtggc
      841 gagcgataac ccagtgcact atgcggatgc cattcgttat aatgctcgaa cgcctctgca
      901 aggttctttg ctgccgttaa cccg
//
LOCUS       DAJTNM010000294          917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTNM010000294 DAJTNM010000000
VERSION     DAJTNM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.301"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..183)
                     /locus_tag="OO172_004258"
     CDS             complement(<1..183)
                     /locus_tag="OO172_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514161.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAP"
     gene            complement(134..>917)
                     /locus_tag="OO172_004259"
     CDS             complement(134..>917)
                     /locus_tag="OO172_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5514162.1"
                     /translation="SEIAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPG
                     AFGFGCAFLPEDVIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLCIRWT
                     DARPETTEPRYRAKSLTFYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVAS
                     SVRNRWGDPDIGGLIIAAYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPSTPTGTYI
                     ASPQFFITGCSEHSLPGSYCALSGVPDAHVSGAMPGLFIRTS"
BASE COUNT          209 a          247 c          233 g          228 t
ORIGIN      
        1 cggtgcgaca tcatcagcgg tggctgtgta ggggactgcc tggtccctgt cgtcaaaaaa
       61 aacgtcacag tctactgcgc cactttcggt ataacgggga ttaatgattt ttttaatttc
      121 cacggtgcat tcctcacgat gtgcgaataa aaagcccggg cattgcgcca gagacatgtg
      181 catccggcac cccggacagg gcgcaatatg accccggtaa tgaatgctct gaacatcccg
      241 taatgaaaaa ttgtggggat gctatatacg ttccggtggg agtactgggc actgaaatcc
      301 ccaccggtcc cagtcgtgag cctctgtatg actgccccct gacaagtctg atgactttat
      361 caccgtcagc ttctccctgg tacgcagcaa taatcagccc gccaatgtca gggtctcccc
      421 atctgttgcg gacagagctc gccacgattc tgtaaataat atcttctgtg gttatattta
      481 ttttcaccca gccagtcagt ctggatatgg gccagtagca gtagcgggta tgataaatgg
      541 ggccgttaat gccgtaaaaa gtaagggatt tggctctgta ccgcggttct gttgtctcag
      601 ggcgtgcatc agtccaccgg atgcagagca ccccttcaaa ccgtgtgtcg ggtatgatga
      661 tgccgtaagg gccagcaacg gaatattcac ctggcagcgc attccttacc caggccagga
      721 aatcactctt agtgtcaaaa cggataacat cttcaggcag aaaagcacac ccaaagccga
      781 atgcgccggg tatcgccaga cggccttttg tccggtcgta aatgtcgctc tgtgcttcca
      841 tcgtggccgc acttttcagc cccagattat cccgggactt ctgttgtgcc ttttcgcctg
      901 ctgctgcgat ttcagac
//
LOCUS       DAJTNM010000295          899 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTNM010000295 DAJTNM010000000
VERSION     DAJTNM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.302"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..534
                     /locus_tag="OO172_004260"
                     /pseudo
     CDS             1..534
                     /locus_tag="OO172_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          260 a          210 c          194 g          235 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggcg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatccaaa aacaaataat
      721 accgttgcaa taattccttt cagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 aaaaattgta caacatatta aaatatgttc atttcgttat aatacacaca agaaaatatt
      841 aagttagata atatattcta aataaccata gataaaaacg cacataatgg ggaatattt
//
LOCUS       DAJTNM010000296          898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTNM010000296 DAJTNM010000000
VERSION     DAJTNM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.303"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(1..898)
                     /locus_tag="OO172_004261"
                     /pseudo
     CDS             complement(1..898)
                     /locus_tag="OO172_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
BASE COUNT          262 a          236 c          239 g          161 t
ORIGIN      
        1 ggtgatacca aagaaggcgt ttacgttcgc gcccgccccc atcgtaaaga agtggtgcag
       61 ccaaacgatg aacgacagca cggtgatgca gacggttgcc cataccagcg aggtataacc
      121 aaacagacgt ttacgcgaga aggttgccgc aatttcggag aacacaccga aaacaggcag
      181 gatcaggatg taaacttccg ggtggcccca ggcccaaatc aggttgatgt acatcatcat
      241 gttgccaccc atatcgttgg taaagaaatg ggtgcccaga tagcgatcca gggtcaacaa
      301 cgcgacggta accgtcagaa ttgggaagga agcaataatc agtacgttcg tgcacagtga
      361 tgcctaggta aatactggca tcttgaacat ggtcatgccc ggtgcgcgca tcttcagaat
      421 ggtaacgaag aagttgatac cggtaagcgt cgtacctata ccggatagct ggagactcca
      481 tatccagtaa tcaacaccga ctcccggact gtactctatt cccgatagcg gtggataggc
      541 cagccagccg gtctgcgcaa attcgcccac gccgagagaa acgttaacca gaatcacgcc
      601 aacaacagta aaccagaagc ttaagttgtt gaggaacggg aacgcaacgt cacgcgcgcc
      661 gatctgcagc ggaaccacca ggttcatcag accgataacg aaaggcatcg ccacgaagaa
      721 gatcataatc acgccgtgcg cggtgaagat ctgatcgtag tggtgaggtg gcaggaagcc
      781 cgcttcaccc gccgaggcaa gagcctgctg gctacgcatc ataatggcgt cagcaaaacc
      841 acgcagcaac atcacaatcg ccacgatgat atacatgata ccgaggcgtt tatggtcg
//
LOCUS       DAJTNM010000297          884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTNM010000297 DAJTNM010000000
VERSION     DAJTNM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.304"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(1..192)
                     /locus_tag="OO172_004262"
     CDS             complement(1..192)
                     /locus_tag="OO172_004262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514163.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASLAEVYWQ"
     gene            239..884
                     /locus_tag="OO172_004263"
                     /pseudo
     CDS             239..884
                     /locus_tag="OO172_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704546.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
BASE COUNT          228 a          245 c          264 g          147 t
ORIGIN      
        1 ctattgccag tacacctcag ccagggatgc cggcacatct gccagtgagg cggcaaccaa
       61 tgcgactgat gctgcaggct cagcacgcgc agccagcacg tcagccggtt cggcagcgtc
      121 atcggcatca tctgcgtctg cttcaaaaga tgaggcgact aaacaggcgt cagcagcgaa
      181 gggcagtgcc acgacggcat cctctaaggc gacagagtct gcaggcagtg cgacggcggc
      241 agcgcagagc aaaagcgcgg cagaatccgc ggcaatgcgc gctgagacag cagcaaaacg
      301 ggcagaggat attgctgatg tcgtatctct ggaagatgcg agcacgacga aaaaggggat
      361 agtgcagctc agcagtgcga ccaacagtgc atccgagtca ctggccgcga cagcgaaagc
      421 ggttaaggtg gtaatggatg aaacaaacaa gaaagcgccc ctgaacagtc cggctctgac
      481 cggaacgcca accacaccca ctgcgccaaa ggggactaat aatactcaga tcgccagtac
      541 ggcttatgtt atggccgcga ttgctgccct cgtggactcg tcgcctgatg cactgaacac
      601 gctgaatgaa ctggcggcgg cgctgggcaa cgacccgaat tttgcgacca ccatgactaa
      661 cgcgcttgcg ggtaagcaac cgaaagatgc cactctgacg gcgctggcgg ggcttgctac
      721 tgcggcagac aggtttccgt attttacggg gaatgatgtc gccagcctgg caaccctgac
      781 aaaagtcggg cgggatattc ttgcgaaatc gaccgttgcc gccgttatcg aatacctcgg
      841 tttacaggaa acggtaaaca aggctgataa cgctgtccaa aaga
//
LOCUS       DAJTNM010000298          882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTNM010000298 DAJTNM010000000
VERSION     DAJTNM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.305"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..658
                     /locus_tag="OO172_004264"
                     /pseudo
     CDS             1..658
                     /locus_tag="OO172_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="antiterminator"
BASE COUNT          259 a          161 c          243 g          219 t
ORIGIN      
        1 gagtggggca acaaaaggtc agcttgaagc ctggcaagag aatgccatgt tcgatacagg
       61 gcgttacagg cgtaaaaaaa tccggtaccg cgatgaagtg actggaaaaa tgataacgcg
      121 ggataatcca ccaatcccgg gaaagcaatc gctggcgaag gggacgtcaa ttcctctggt
      181 cagtccggtt gagttttcga catcatcgtg gcggcgggct gttctgtctc ttgaagaaca
      241 tcataaagcc tggttgttgt ggtgttacag cgggagtatt tgttgggaat atcagatcgc
      301 gataacacag tgggcgtgga atgaatttaa tgctcaatcc ggtaccagaa aaattgcagg
      361 gaaaacgcag gaacgcctga aaaaattaat ctggctggcg gcgcaggcag taaaagcaga
      421 actttttggt ggggaaggtt atgaatacca ggttggtggg gaaggttatg aataccagga
      481 gctggcattt actggcggga gtgacaacta aaaactggtc caaaacattt actcgtcact
      541 ggattgcaat gaaacacatt tttcaccgac tggatagtga ggctttattg tttgtaatga
      601 gaacacgttc aaaaaaaaga cggcattttc aaagcaaagt gttgcaaaag tagattgaaa
      661 ggcatatatt catgcaaatc tgatattttg ccgatttttt acgtgatggc aaaagcaaac
      721 aaaacccgcc cacaagcggg ttttttgtgc cacttatctc ggatagacat ggtgaatgcg
      781 ctggtggagg agataagggt gatttttgaa tgcttgcaac attgatttcg taacgttatt
      841 atcctgcgcc cggcccttta gctcagtggt gagagcgagc ga
//
LOCUS       DAJTNM010000299          869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTNM010000299 DAJTNM010000000
VERSION     DAJTNM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.306"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
BASE COUNT          242 a          210 c          157 g          260 t
ORIGIN      
        1 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg
       61 taagaggcta acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct
      121 tgcaggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
      181 agacagatag cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg
      241 tttttgtccg gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca
      301 gtattcaatg cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa
      361 cattaatatc aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca
      421 tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc
      481 tacaatcaat gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct
      541 aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa
      601 aacattcccc atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa
      661 cctataatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca
      721 ttaatctggt tgccggatgc tggtggattt tcgcgtttta gttgttcata aaagtgcaca
      781 gctttaacca gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt
      841 tcatagttcg gcctgtcgtt acaatcctc
//
LOCUS       DAJTNM010000300          860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTNM010000300 DAJTNM010000000
VERSION     DAJTNM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.307"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>860
                     /locus_tag="OO172_004265"
     CDS             <1..>860
                     /locus_tag="OO172_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514164.1"
                     /translation="AHPIHGRPFSPEVTFVIDGGTRFVVGWSLDLAENVFAVAGAIQH
                     GIRNHGKPFLYYSDNGSGETADMLDKEIVGILPRLGINHPTGIAGNPQGRGIIERLNR
                     TLPMRIARRYRTYFGKGADRETLRVLNRDLRSAFNALQQDKPLNARQKSAMRELPSWS
                     ELIEAIREGVEWYNNRPHSELPMKPNGKHYSPAEFRKKRLAEENTEIEWLSDLELRDM
                     FCPMVERPVRRCEIKWLGNIYYAPELRDEHGRKVLISYDIHDAERITVRRKDGSFICE
                     AIWNGNKRAA"
BASE COUNT          216 a          208 c          245 g          191 t
ORIGIN      
        1 tgcgcatccg atccacgggc gtccattttc accggaagtc acgtttgtta tcgacggtgg
       61 aacccggttt gttgttggct ggagtcttga tcttgcggag aacgtgtttg ctgttgccgg
      121 ggcgatacag cacggaattc gcaatcacgg aaaacccttc ctgtattact cggataatgg
      181 ttccggtgaa acggcagata tgctggataa agagatcgtc gggattctgc cccgtctggg
      241 tattaaccac cccaccggta ttgccggaaa tccgcaggga cgcggcatca ttgaacgtct
      301 gaaccggacg ctgccgatgc gtatagccag acggtatcgc acctattttg gcaagggggc
      361 agaccgggaa acactgcgtg tactcaatcg cgatctgcgt tcagcattta atgccctgca
      421 acaggacaaa ccgctgaatg cccgccagaa atctgcaatg cgcgagctgc cgtcctggtc
      481 agaactgatt gaggctattc gtgaaggagt ggaatggtac aacaaccgcc cgcattctga
      541 actgccgatg aaaccaaacg ggaagcatta cagcccggct gagttcagaa aaaaacgcct
      601 ggcggaagaa aacaccgaaa ttgaatggct gagtgatctc gaactgcgcg acatgttctg
      661 cccgatggtg gagcgtccgg taagacgctg tgaaatcaaa tggcttggga atatttatta
      721 cgcgccggag ctgcgtgatg aacatggtcg caaagtgctt atcagctatg acattcatga
      781 tgcagagcga attacagtgc gccgtaagga tggcagtttt atttgtgagg cgatatggaa
      841 cggcaataaa cgcgccgcct
//
LOCUS       DAJTNM010000301          854 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTNM010000301 DAJTNM010000000
VERSION     DAJTNM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.308"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..108
                     /locus_tag="OO172_004266"
     CDS             <1..108
                     /locus_tag="OO172_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5514165.1"
                     /translation="EDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCNA"
     gene            complement(148..>854)
                     /gene="yqhC"
                     /locus_tag="OO172_004267"
     CDS             complement(148..>854)
                     /gene="yqhC"
                     /locus_tag="OO172_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5514166.1"
                     /translation="RVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQ
                     ELLMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREI
                     LYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFH
                     HNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFG
                     VTPGEDAARMRAMQGN"
BASE COUNT          194 a          241 c          207 g          212 t
ORIGIN      
        1 gaagatcagg taatgacgtt cctgatgatc ctgccaattg ccttgttaac cgctggcttg
       61 ttaggcacgc tgtttgtggt gattaaaaaa aaatactgta acgcctgacg attttccccg
      121 ttcccggctg ctgtaccggg aacgtattta attcccctgc atcgcccgca ttcttgccgc
      181 atcttccccc ggcgtcacac cgaagtaacg tttaaactca cggctaaatt gcgatgcgct
      241 ttcatagccg acgcgcatcg ctgctgcgct ggccttcatg ccgtcatgga tgatcatcat
      301 ccgcgcctta tgcagacggt aattcttcaa atactgcaac ggcgaggtgc tggtgacaga
      361 cttaaaatta tggtggaacg ccgatacgct catgttggct tctgccgcca gttgctcgac
      421 gctcaggttt tcggtgtatt tattctcaat tcgtttcagc acgcggctaa tcagactgaa
      481 gtgagtctgg cgactgacca gcgccagtaa cgcgccgccg caaggtccgg tcagcacgta
      541 gtacagaatt tcgcggatga tctgtttgcc gagaatacgc gcatccagtg gtcgctccat
      601 cacgtcgagt aaccgctccg ccgcgaataa aatctcttct gataacgtgg cggagttaat
      661 cccgctggct gccatcgacg gctggaaatg ctcatcttcg ccaatgtcca tcaacagttc
      721 ctgtaactgc aaaatatcga cattgagacg caaccctgcc agcggcacct ctgacgtggc
      781 ataggtttcg cactcaaacg gcaacggcac cgtcagcagc aggtattcat tggcatcata
      841 acgaaacacg cgtt
//
LOCUS       DAJTNM010000302          833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTNM010000302 DAJTNM010000000
VERSION     DAJTNM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.309"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            56..475
                     /gene="rbsD"
                     /locus_tag="OO172_004268"
     CDS             56..475
                     /gene="rbsD"
                     /locus_tag="OO172_004268"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5514167.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            483..>833
                     /locus_tag="OO172_004269"
     CDS             483..>833
                     /locus_tag="OO172_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5514168.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKT"
BASE COUNT          230 a          199 c          207 g          197 t
ORIGIN      
        1 actcagtcag gattaagctg cggatcagcg aaacgtttcg ctgatggaga aaaaaatgaa
       61 aaaaggcacc gttcttaatt ctgatatttc atcggtgatc tcccgtctgg gacataccga
      121 tacgctggtg gtgtgtgatg ctggtttacc catccccaaa agtacaacgc gtatcgatat
      181 ggcattaacc caaggtgtac cttcttttat gcaggtgctg ggcgtcgtca caaatgaaat
      241 gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa cataatccgc aactccacga
      301 aacgttgctc actcaccttg agcagctgca aaaacaccag ggaaatacca ttgaaattcg
      361 ttacaccacg catgaacaat tcaaacaaca aaccgcagaa agtcaggcgg taattcgcag
      421 cggagaatgt tctccgtatg cgaatatcat tctctgtgct ggcgtgacgt tctgaggccg
      481 tcatggaagc attacttcag cttaaaggca tcgataaagc cttcccgggt gtaaaagccc
      541 tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat ggcgctggtg ggcgaaaacg
      601 gcgcgggtaa atccaccatg atgaaagtgc ttactggtat ctatactcgc gatgccggca
      661 cgcttttatg gctggggaaa gaaacgacat ttaccgggcc gaaatcttcc caggaagccg
      721 ggattgggat tatccatcag gaactgaacc tgatcccgca gttgaccatt gccgaaaaca
      781 ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat tgactggaaa acc
//
LOCUS       DAJTNM010000303          822 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTNM010000303 DAJTNM010000000
VERSION     DAJTNM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.310"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(13..378)
                     /locus_tag="OO172_004270"
     CDS             complement(13..378)
                     /locus_tag="OO172_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5514169.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            complement(379..>822)
                     /locus_tag="OO172_004271"
     CDS             complement(379..>822)
                     /locus_tag="OO172_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5514170.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPES
                     PAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVI
                     PLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
BASE COUNT          177 a          225 c          194 g          226 t
ORIGIN      
        1 aatactggtt attcatgcgc accccagttc tgtgattttt atccccagcc gcccaccagg
       61 aacgagctga ccgcgcacaa tattgatttc atcaaactgc tcgtcgtcta taagtagtcc
      121 agcatgcgtc agcgcatcca gcggtgcctt caggatattg tccaggtcac gacggcgctt
      181 atccggtggc tctgcaataa tctttatcgc cagccttccg gacaggttta atttcagtcg
      241 ctgctggcga acaattagcg ccacatcacg gcgataacgc tcaccggctt ttgatacaaa
      301 atatgtgctg ccacgacgtc gccagtaggt gttcaccgtt ggcgggtaag gcaaaacaaa
      361 ttctatgcgt tcggtcattt atgctttcca cttcagaaca cccgaatttc tcgcgtgcat
      421 taaaaaacga atcagcaaca acaactgact accgtgtttt tcttcaaaat cttttacccc
      481 ggcgtgtagt tcgttatggc atttacgaca cagcggaata acaaacaaat cgtcagcctt
      541 tgttcccatc cctcccagtc catgaccaat gatgtggtgc ggatcatctg cctgattgcc
      601 acacgtcatg catttctgcg tttttaccca gcgcgtgtat acaggcatct cttcccgttg
      661 tggtttctgg cgctggagat actgagccgg agattccgga tcaacggcaa tgctgaccac
      721 cgtctcttcc tgtggtggat tctgttgctg gtgggcgtga ggcagcggcg caagattttt
      781 tgtgcgctgc ttcagtatgc tggtggcggt ctgctctccc gg
//
LOCUS       DAJTNM010000304          819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTNM010000304 DAJTNM010000000
VERSION     DAJTNM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.311"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            194..>819
                     /gene="argF"
                     /locus_tag="OO172_004272"
     CDS             194..>819
                     /gene="argF"
                     /locus_tag="OO172_004272"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5514171.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQ"
BASE COUNT          207 a          207 c          199 g          206 t
ORIGIN      
        1 cgctgttctt ccagttgttc cgggtttgcc atggctcatt cctcttaatg tcctgccgat
       61 actcttattg tcacacacca ctgacattgc ctccaccttt gtgacaaaga tttatgcttt
      121 agacttgcaa atgaataatc atacacataa atttaatttt aattcattga ggcgttagcc
      181 acaggaggga tctatgtccg ggttttatca taagcatttc ctgaaattac tcgatttcac
      241 gccagctgaa ctcaacagcc tgctgcagtt agccgcgaag ctgaaagctg ataagaaaag
      301 cggtaaagaa gaagccaaac tcactggtaa aaacatcgcg ctcatcttcg aaaaagactc
      361 gactcgtacc cgatgctctt tcgaagttgc cgcatatgac cagggtgctc gcgttactta
      421 tcttggccca agcggtagtc agattggtca taaagagtcg attaaagaca ctgcccgcgt
      481 gcttggtcgc atgtatgacg gtattcagta tcgcggctat ggtcaggaga ttgtcgaaac
      541 actggcggaa tacgctggcg tgccggtatg gaacggcctg accaatgagt ttcaccccac
      601 gcagttgctg gcggatctcc tcaccatgca ggagcattta cccggtaaag cattcaacga
      661 aatgacgctg gtctatgcag gtgacgcacg taacaacatg ggcaattcga tgctcgaagc
      721 tgcggcgctt accggtctgg atttgcgtct ggtcgcgcca caggcatgct ggccagaagc
      781 tgcgctggtt acggaatgcc gcgccctggc acaacaaaa
//
LOCUS       DAJTNM010000305          819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTNM010000305 DAJTNM010000000
VERSION     DAJTNM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.312"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..236
                     /locus_tag="OO172_004273"
     CDS             <1..236
                     /locus_tag="OO172_004273"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514172.1"
                     /translation="FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTE
                     REFATILAGISAGGMYCPECGTVHWPDGVAPPF"
     gene            236..>819
                     /locus_tag="OO172_004274"
     CDS             236..>819
                     /locus_tag="OO172_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5514173.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          172 a          211 c          245 g          191 t
ORIGIN      
        1 gttttgacga tgccgttgaa tgctgcgcag acctgaatat tccgttcttt gtggatgcgg
       61 gaaacaaaaa gctggtcttc tggtttgtac gtgttgatga cgaagggtat ccggaaatcg
      121 cccgctgcac ggagcgggaa tttgcgacca ttcttgccgg tatcagcgcc ggcggtatgt
      181 actgcccgga gtgtggcacg gttcactggc cggacggagt cgccccgccc ttctgatgct
      241 tccccgtttt gccgatattt ttcagcaggg aaaccgctgg cttaactggc tggagaaaca
      301 accggaaggg gcagtccgtc cggtggtaat tgaatcagtg acaaaaatca tggcctgcgg
      361 gaccacactg atgggctaca cgcagtggtg ttgttcatca ccggactgtt gccatacaaa
      421 aaaggtctgc ttccggtgta aaagccgctc ctgcccgcac tgcggggtga aggctggcgc
      481 acagtggata cagtatctgc tgagcctggt ccccgactgc ccgtggcagc atattgtgtt
      541 cacacttccc tgccagtact ggtccctggt gttccacaac cggtggttac tggcagagat
      601 gagccgcatt gcagcggatg tgatactgga aatctgccat caggcagatg tggagccggg
      661 gatattcacg gtgatccaca catgggggcg tgaccagcag tggcatccgc atatccattt
      721 atcgacaact gccggtggtg tgacgtcggg ccacacctgg aaaaatcttc atttttacgc
      781 ccgtaaggtg atgagcatgt ggcgctaccg gataacacg
//
LOCUS       DAJTNM010000306          794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTNM010000306 DAJTNM010000000
VERSION     DAJTNM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.313"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..362)
                     /locus_tag="OO172_004275"
     CDS             complement(<1..362)
                     /locus_tag="OO172_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514174.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHMKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWH"
     gene            complement(364..642)
                     /locus_tag="OO172_004276"
     CDS             complement(364..642)
                     /locus_tag="OO172_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514175.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDSCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT          188 a          207 c          230 g          169 t
ORIGIN      
        1 cagtgccagc acaacaccat tgcgccggta ccataacggt gaatgacggt ttcgctgtga
       61 tgataatcgc cgtgtggcca ctggcaggat ttcatatggc gcaacagcca gtcagacaat
      121 gcgccagcac caccagcagc acgaatcacc cgtgcgttac tgaaaaacgg cagcaatgtt
      181 ttgtcttcca ctagcggctg gcgaacggca ggaacgaccc cggacggcag attacgcatg
      241 cttttcggtt ccggctccac cagtacccgg gtattgtgga ataccggcat ggattcacgg
      301 cctggcttaa ggaccaccag cccgagttcc ggaaccagaa caggtcgaag taatacccgc
      361 acattacctc cagatccgtt gctggaatgt gcaggacgga cgcggtggcc gttcggaata
      421 agggagcctg acgaagatta tccagtgacg ataatcgaga ctgagggctt tcttaatctc
      481 gtatccgtgt ctgcggtagc gctgaatcag ccattcagcc tgttcttcgg tgcaggagtc
      541 gtgctgatac cagtcagatt tgaatgcatg agaacgccgc ccgtgcctgc tggcaggggc
      601 ggcagagtta tccgaattgt aaaatttggt atcgtgcgcc atctgttttc tctgctggcg
      661 cagcaggtgc cagttgttca ggctgacgga tggattgtaa accagaacga ccagaaaaaa
      721 caaaacccgc cgaagcgggt taagtgcggg tgcgttgagg atgcctgaca catcagcggt
      781 ggcgagggat ttct
//
LOCUS       DAJTNM010000307          772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTNM010000307 DAJTNM010000000
VERSION     DAJTNM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.314"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(133..207)
                     /locus_tag="OO172_004277"
     tRNA            complement(133..207)
                     /locus_tag="OO172_004277"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(172..174),aa:Gly,seq:tcc)
     gene            complement(211..286)
                     /locus_tag="OO172_004278"
     tRNA            complement(211..286)
                     /locus_tag="OO172_004278"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Thr,seq:tgt)
     gene            complement(289..365)
                     /locus_tag="OO172_004279"
     tRNA            complement(289..365)
                     /locus_tag="OO172_004279"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(329..331),aa:Arg,seq:tct)
     gene            complement(367..443)
                     /locus_tag="OO172_004280"
     tRNA            complement(367..443)
                     /locus_tag="OO172_004280"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(407..409),aa:Ile,seq:cat)
BASE COUNT          210 a          195 c          178 g          189 t
ORIGIN      
        1 actgagacct taggtctgct cactacttca acaaagttag tcacagtgtt ggttacagtg
       61 agtaaaacta tcggaaggcg ttaagccgta tcccttagga ttcagtaagt tagtagactt
      121 taagaagtgt catggagcgg gcgaagggaa tcgaacccgc atcatcagct tggaaggctg
      181 aggtaatagc cattatacga tgcccgcata tggtgccgac taccggaatc gaactggtga
      241 cctactgatt acaagtcagt tgctctgcct gctgagctaa gtcggcgctg gcccaccacc
      301 gaggactcga acctcgcacc gtcaacttag aaggttgatg ctctatccgg atgagctagt
      361 ggtggttggt ggcccttgct ggacttgaac cagcgacctg gcgattatga gtcgctcgct
      421 ctgaccaact gagctaaagg gccggaggca gaataataac catatgtcat cacatctgca
      481 aactcatctg accaccagcg cgtttaacgc cctgtgccat ttttcaggca caaaaaaacc
      541 cgcataaagc gggttctttc aagtgtccat gtctgctatt cgcctcgcga tacagctttg
      601 cgaagcttac cggaattgaa gcagtttatg tgcaaaaatg caagaacttt tttaaagctg
      661 catcaacctt tccaccagtt tatctctgcg aacaacaaac caaccattgg ctctcgccag
      721 ttccagccat gactcaaggg aaataacaat atcatcatcc cgtaactgaa tt
//
LOCUS       DAJTNM010000308          771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTNM010000308 DAJTNM010000000
VERSION     DAJTNM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.315"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            26..771
                     /locus_tag="OO172_004281"
                     /pseudo
     CDS             26..771
                     /locus_tag="OO172_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          199 a          185 c          216 g          171 t
ORIGIN      
        1 cagacaacca gtatcactta tttaagtgat agtcttaata ctagttttta gactagtcat
       61 tggagaacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt gtagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtggccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagctt agtcagccgt tgtctccggg tgtcgcgtgc gcagttgcac
      481 gtcattctca gacgaaccga tgactggatg gatggccgcc gcagtcgtca cactgatgat
      541 acggatgtgc ttctccgtat acaccatgtt atcggagagc tgcccacgta tggttatcgt
      601 cgggtatggg cgctgcttcg cagacaggca gaacttgatg gtatgcctgc gatcaatgcc
      661 aaacgtgttt accggatcat gcgccagaat gcgctgttgc ttgagcgaaa acctgctgta
      721 ccgccatcga aacgggcaca tacaggcaga gtggccgtga aagaaagcaa t
//
LOCUS       DAJTNM010000309          770 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTNM010000309 DAJTNM010000000
VERSION     DAJTNM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.316"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..183)
                     /locus_tag="OO172_004282"
     CDS             complement(<1..183)
                     /locus_tag="OO172_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514176.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNG"
     gene            complement(319..>770)
                     /locus_tag="OO172_004283"
     CDS             complement(319..>770)
                     /locus_tag="OO172_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HCR5514177.1"
                     /translation="PLLFRPREVQGTMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIV
                     THLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYED
                     TMLTMEHIRKPLAARQVLSRSETVLQKSLEITEEADLALRNAMARIK"
BASE COUNT          192 a          206 c          164 g          208 t
ORIGIN      
        1 tccgttgtaa atggcgctgg aaaatttctc atcagtgaaa ccttccggaa atttcccccc
       61 cttgtatgat gtatatcgcc agtattttat ttcatcccgg atataatttt tatatttccc
      121 ggcacgtgca ataacgctga ccagagatat aaggctcatt aataacttta caggtaattg
      181 cataattaaa tttccagtga gtgaattgac aggtgagggg taaaactttc tctcaggtaa
      241 tatatatctg aaaatatttt caataacacc agctaggaaa aacaccacca caggcggtgg
      301 cgtttctttg gagcgtcttt atttaatccg ggccatcgcg tttctcagtg ccaaatctgc
      361 ctcttccgtt atctccagac ttttctgcaa caccgtttct gaccgggaca gtacctgacg
      421 tgctgccagc ggtttccgga tatgctccat cgtcagcatc gtgtcttcat agccctgctg
      481 acgccaggcg tcggtatgtg ccaacgtgaa actgagcaga ccgccgctgt tgtcaccatc
      541 acctgcatat ttcagccttt tccggggacg gatctcaagg attgtggtgt ccggaaaagc
      601 ctggcgatcc cataaagaac cgtcactcag atgcgtcaca atcaccatat tgcatccggc
      661 atcgaccaga ggcgtcacag gggtatttcc ctgcatattt cgccatcctc ccataccacc
      721 atcaccgaac attgtcccct gaacctcacg gggacggaac agcaggggca
//
LOCUS       DAJTNM010000310          742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTNM010000310 DAJTNM010000000
VERSION     DAJTNM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.317"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..255
                     /locus_tag="OO172_004284"
     CDS             <1..255
                     /locus_tag="OO172_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5514178.1"
                     /translation="NDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDK
                     EKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            279..>742
                     /locus_tag="OO172_004285"
     CDS             279..>742
                     /locus_tag="OO172_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5514179.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGT"
BASE COUNT          198 a          172 c          216 g          156 t
ORIGIN      
        1 aatgatgaat ttgtctttga ctacagactc aaagcggaag atgagcgtga tgcccttctg
       61 gctcaggtca gtgcccggac aggggaatgg gaagaagacc tgctgctggg gttaatcagc
      121 gacgaagata aagaaaagct gaaagcctgt cgtatttacg cgaaatcgct gcaggcgatg
      181 gatttcagca ccatcactga taaatcctca tacaacgcca ttgaatggcc cgcctctccg
      241 gaaggttctt cctgatttaa tttatcgcga gaaaaacaat gtctgtagtg atatcaggtg
      301 cgctgactga tggcgcaggt atccccatgt ccggatacca tattattctg aaatcccggg
      361 taaacacccc ggaagtggtg atgaacactg ttgctgatgt gatgacagga aacgatggtg
      421 aatactgttt ccatgcgcgg actggaaaat atggtgtgta tctgaaacag gactggcgca
      481 acgagtacaa cgttggcgac attgctgtat atgaggactc aaagcccggc acgctgaatg
      541 actttctgat tgctcctgat gagggcgacc tgaaaccgga tgtcgtcaaa cgctttgagg
      601 aaatggtggc gcaggcgcag cagagcgccg gggccgcagc cggaaacgca cagcagacgg
      661 cgcaggatgt ggcggcagcc gcaggttatg cccgcgcagc agagcaggcc aaaaatgaca
      721 ttgatgctgc gctgaccggc ac
//
LOCUS       DAJTNM010000311          737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTNM010000311 DAJTNM010000000
VERSION     DAJTNM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.318"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>737
                     /locus_tag="OO172_004286"
     CDS             <1..>737
                     /locus_tag="OO172_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HCR5514180.1"
                     /translation="TMYQGRIVFHSAMLWTIGFIVTFSVGGMTGVLLAVPGADFVLHN
                     SLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNETWGKRAFWFWIIGFFVAFMP
                     LYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALGILCLVIQMYVSIRDRDRDQN
                     RDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWEMKEKGEAYKKPDHYEEIH
                     MPKNSGAGIVIAAFSTIFGFAMIWHIW"
BASE COUNT          141 a          198 c          203 g          195 t
ORIGIN      
        1 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
       61 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
      121 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
      181 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
      241 tgaacgaaac ctggggtaaa cgcgcgttct ggttttggat catcggcttc ttcgttgcct
      301 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
      361 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
      421 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cgcgaccaga
      481 accgtgacct gactggcgac ccgtggggtg gccgtacgct ggagtgggca acctcttccc
      541 cgcctccgtt ctataacttt gccgtagtgc cgcacgttca cgaacgtgat gcattctggg
      601 aaatgaaaga gaaaggcgaa gcgtataaaa agcctgacca ctatgaagaa attcatatgc
      661 cgaaaaacag cggtgcaggt atcgtcattg cagctttctc caccatcttc ggtttcgcca
      721 tgatctggca tatctgg
//
LOCUS       DAJTNM010000312          716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTNM010000312 DAJTNM010000000
VERSION     DAJTNM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.319"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            60..>716
                     /gene="istA"
                     /locus_tag="OO172_004287"
     CDS             60..>716
                     /gene="istA"
                     /locus_tag="OO172_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR5514181.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHHNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     P"
BASE COUNT          168 a          187 c          185 g          176 t
ORIGIN      
        1 cacccaacgc ctgaaatcag atccaggggg taatctgctc tcctgattca ggagagctta
       61 tggtcacttt tgagacagtt atggaaatta aaatcctgca caagcaggga atgagtagcc
      121 gggcgattgc cagagaactg gggctctccc gcaataccgt taaacgttat ttgcaggcaa
      181 aatctgagcc gccaaaatat acgccgcgac ctgctgttgc ttcactcctg gatgaatacc
      241 gggattatat tcgtcaacgc atcgccgatg ctcatcctta caaaatcccg gcaacggtaa
      301 tcgctcgcga gatcagagac cagggatatc gtggcggaat gaccattctc agggcattca
      361 ttcgttctct ctcggttcct caggagcagg agcctgccgt tcggttcgaa actgaacccg
      421 gacgacagat gcaggttgac tggggcacta tgcgtaatgg tcgctcaccg cttcacgtgt
      481 tcgttgctgt tcccggatac agccgaatgt tgtacatcga attcactgac aatatgcgtt
      541 atgacacgct ggagacctgc catcataatg cgttccgctt ctttggtggt gtgccgcgcg
      601 aagtgttgta tgacaatatg aaaactgtgg ttctgcaacg tgacgcatat cagaccggtc
      661 agcaccggtt ccatccttcg ctgtggcagt tcggcaagga gatgggcttc tctccc
//
LOCUS       DAJTNM010000313          706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTNM010000313 DAJTNM010000000
VERSION     DAJTNM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.320"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>706
                     /locus_tag="OO172_004288"
     CDS             <1..>706
                     /locus_tag="OO172_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5514182.1"
                     /translation="DLHELFKTVRSLGIISVVVGVPGVGKTFAAREYCRRTANAWMVT
                     LAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGRVNPLLIIDEA
                     DHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSRFAMTKQLRKV
                     KKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAKGAGTSLNKSH
                     IKAAFKEAYSNPRLLNW"
BASE COUNT          191 a          153 c          202 g          160 t
ORIGIN      
        1 gatttgcatg agctgtttaa aacagtgcgc tcactcggga ttatcagtgt cgttgttggc
       61 gtaccgggtg tcggaaaaac ctttgctgcc cgggaatatt gtcgcaggac agctaatgcc
      121 tggatggtga ctcttgctcc ggcacattcg agcgtaacgg aatgcctcct tgaaatagca
      181 tatgcgctgg aaattaataa tccgggaaga aataagggga atataacccg cgccattcgc
      241 agaaaactgg gtggtggtaa agaaaccagt ggccgtgtta acccccttct gattattgat
      301 gaagctgatc acttaagtgt ggatggtctg gagcaattac gcgccattca ggatgccact
      361 ggcgtgggga tggttttaat tggcaacccg aaacagatgg cggatgccac ccgtcgtggg
      421 actgatgaac tggcccgtct gttcagccgt tttgccatga caaaacaact gcgcaaagta
      481 aaaaagaccg atgtgatggc tgtagccaaa gcatggggag taacacagga agatgagctg
      541 gacctgctga tgagggtagc cgaaaagccc gggggattgc gtgtattaac tcacacactg
      601 aatcatgcgt ggctggcggc aaaaggtgca ggcacatccc tgaataaaag tcacattaaa
      661 gcggctttta aggaggctta cagcaacccc aggttactga actggt
//
LOCUS       DAJTNM010000314          702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTNM010000314 DAJTNM010000000
VERSION     DAJTNM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.321"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..244
                     /locus_tag="OO172_004289"
                     /pseudo
     CDS             1..244
                     /locus_tag="OO172_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          164 a          157 c          218 g          163 t
ORIGIN      
        1 aaaggcaact aataaagata cagataaaga tctaaaaaca gatctaaccc atcccaaacc
       61 cttcccttcc ggaagggagt ttcgggattt tgtggctgga gtgcttgagg ggagattatc
      121 tggcggtact gcagcggaat tttgtaattc tgcggtggtt gcgttgcagg ctgctggcct
      181 ggatgtctgt cgtgagtatc cggtgccaga gcgtggtgac ggttgcggag ggcggattga
      241 tatcgtcgtg actgacagga acggtgtccg gtgtgggatc gagcttgacc gaaattctcc
      301 gcgacagaaa tcactgctca aaatcggtgc tgttgaaacc gggatatgtg tcttgcggcg
      361 cagtgatatc gcaaggcaca ccgagcaggg aattctggtt atcggtgggg ctgttcgcca
      421 gaaaaaattt gacccgttgt cagttgatct gcccgactgg ttgccagata cactctggca
      481 tgagtgggtc cagttcaggc aggcattgcg aaaaccgatt cgaacggagc tgggcgctaa
      541 cggggcgata cgggaactgg aaaaattccg tcagcagggt tttacacctg agcaggtgat
      601 tcgacacagc atcgccaatg aataccaggg tctgttcgcg ccgaaaggtg ttcggcctga
      661 gacgttgctc cgacaggtta acaccgtctc gttgccggac ag
//
LOCUS       DAJTNM010000315          702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTNM010000315 DAJTNM010000000
VERSION     DAJTNM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.322"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(1..346)
                     /locus_tag="OO172_004290"
                     /pseudo
     CDS             complement(1..346)
                     /locus_tag="OO172_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(394..>702)
                     /locus_tag="OO172_004291"
     CDS             complement(394..>702)
                     /locus_tag="OO172_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5514183.1"
                     /translation="VGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGVGIRI
                     FNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTVEYKK
                     "
BASE COUNT          207 a          150 c          136 g          209 t
ORIGIN      
        1 caggcaatga cacacctttg gtgtgaataa tgcgtaatat tttttcttgc gaaggttcta
       61 atcgtgatac tggcggaata acggtaaacg gcgctttgct tttatcctct ttattaaatg
      121 gatctaccca agcctggatg agataaggta ttttttcttc tttgctaaat acagaaatag
      181 atttatcagc attattactt aagtaaacca cccgcgtact accaatgaca acgcctgcat
      241 aagttaatgc tggttggcct aaaaaaacgc tgagtataat cgcggtaaat aacttacatt
      301 aaactgttgt tttaaagagc ctgattaaac aactttttct ggacataacg actccctggc
      361 caaagccaat tgaataaaaa ggcccttgat aaattatttc ttatattcga cagtaaattg
      421 tgaatcagcg ttcgccgtac cagccgttac ggtcgcattg gttgccacat agcgtgcaat
      481 aaacttcatt tcagccttgc cttcagcatc gatagcggta taatcagagt cgttataaag
      541 tttaacctgc gaattatcgg actggttaaa aatacgaata ccaacgccgg ttgccgcaat
      601 cgcttgtcct gtaccatcaa aacgaacggc tgcttgagtg tacgttgccg ggcaattttc
      661 aagtttaata ctgaacttct gtggcgacgc cagactacct ac
//
LOCUS       DAJTNM010000316          697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTNM010000316 DAJTNM010000000
VERSION     DAJTNM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.323"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..415)
                     /locus_tag="OO172_004292"
     CDS             complement(<1..415)
                     /locus_tag="OO172_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5514184.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCL"
     gene            507..593
                     /locus_tag="OO172_004293"
                     /pseudo
     CDS             507..593
                     /locus_tag="OO172_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          196 a          131 c          181 g          189 t
ORIGIN      
        1 gcaaacatgt taaaactctg ttaagtgaaa catcaagagc gactaggtga ggcggaagag
       61 ccgggagcct gctcagtctg ttatcgctgg ctttaagtac agttaaggac ggagggagtt
      121 ctggcagaga gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt
      181 atgggagaga gcgtaatctg agtaaactta aattgagggc tggctcttga aaagccagac
      241 atattttcag taatcgaact gcctgtgttc ggtcttctgt tgcagcacct tccttggccc
      301 agttatccca gatgcggtga taatccgtga tattttgctc ttctacggat aaacgggata
      361 tacatcctaa accacaaaag gagcggaaaa aagaaggatt gtggacaggt ttcatttatt
      421 attgatcctc attgtttata tattaatata ttcgtgcaaa tggagtgagg attctttaaa
      481 aaaacgcgcc tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa
      541 atgaaggggt gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc
      601 cctacctttt catcaagcca gtagtctgcc caccacagca tcatttctct gcgcttatcg
      661 agatactgag catggtcgta aattcagcta acccagt
//
LOCUS       DAJTNM010000317          695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTNM010000317 DAJTNM010000000
VERSION     DAJTNM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.324"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>695
                     /gene="pyk"
                     /locus_tag="OO172_004294"
     CDS             <1..>695
                     /gene="pyk"
                     /locus_tag="OO172_004294"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5514185.1"
                     /translation="KTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSV
                     IGNSEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKG
                     VNLPGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENI
                     HIISKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKV
                     VITATQMLDSMIK"
BASE COUNT          199 a          163 c          169 g          164 t
ORIGIN      
        1 aaaactggta aaaccgccgc tatcctgctt gataccaaag gtccggaaat ccgcaccatg
       61 aaactggaag gcggtaacga tgtttctctg aaagctggtc agacctttac tttcaccact
      121 gataaatctg ttatcggcaa cagcgaaatg gttgcggtaa cgtatgaagg tttcactact
      181 gacctgtctg ttggcaacac cgtactggtt gacgatggtc tgatcggtat ggaagttacc
      241 gccattgaag gtaacaaagt tatctgtaaa gtgctgaaca acggtgacct gggcgaaaac
      301 aaaggtgtga acctgcctgg cgtttccatt gctctgccag cactggctga aaaagacaaa
      361 caggacctga tctttggttg cgaaaaaggc gtagactttg ttgctgcttc ctttattcgt
      421 aagcgttctg acgttatcga aatccgtgag cacctgaaag cgcacggcgg cgaaaacatc
      481 cacatcatct ccaaaatcga aaaccaggaa ggcctcaaca acttcgacga aatcctcgaa
      541 gcctctgacg gcatcatggt tgcgcgtggc gacctgggtg tagaaatccc ggtagaagaa
      601 gttatcttcg cccagaagat gatgatcgaa aaatgtatcc gtgcacgtaa agtcgttatc
      661 actgcgaccc agatgctgga ttccatgatc aaaaa
//
LOCUS       DAJTNM010000318          695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTNM010000318 DAJTNM010000000
VERSION     DAJTNM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.325"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>695
                     /locus_tag="OO172_004295"
     CDS             <1..>695
                     /locus_tag="OO172_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5514186.1"
                     /translation="RADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGAFAIENGLIAAT
                     SPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVALTFLNAAGTKT
                     GKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELDADKALLARIES
                     IRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRALAITGAIAISS
                     SCALEGTVTRQIV"
BASE COUNT          165 a          164 c          182 g          184 t
ORIGIN      
        1 cgcgtgctga tgtcgattat ctgtttgctc aagttatcgt ccatgagcaa cgtgtcgata
       61 ccacgcctaa ctgcggcaat atgctgtctg gcgtaggggc attcgccatt gaaaatggtt
      121 tgattgcagc gacttcgcca gttacccgtg tacgtatccg caacgtcaat acgggtacgt
      181 tcatcgaagc tgatgtgcaa acgccaaatg gtgttgtcga gtacgagggt agcgccagaa
      241 ttgacggcgt accgggtact gccgcaccgg ttgcgctcac tttcctgaat gccgctggaa
      301 cgaaaaccgg aaaagttttc ccgactgata atcagattga ttattttgac gatgtcccgg
      361 tgacctgtat cgatatggcg atgccagtcg tcattattcc ggctgaatat ctgggaaaaa
      421 caggttatga attaccggcg gaactggatg ccgacaaagc attattagcc cgcattgaat
      481 ctatccggct acaagcgggt aaagcaatgg gcttaggtga tgtcagtaat atggttatcc
      541 ctaaacctgt gcttatttct ccagcgcaga aaggcggggc aattaatgtg cgttatttta
      601 tgccgcattc ttgccatcgc gcgctggcga taaccggtgc tattgctatt tccagtagtt
      661 gtgcattgga aggcaccgtc acccgacaaa tcgtc
//
LOCUS       DAJTNM010000319          692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTNM010000319 DAJTNM010000000
VERSION     DAJTNM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.326"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            57..554
                     /locus_tag="OO172_004296"
                     /pseudo
     CDS             57..554
                     /locus_tag="OO172_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            595..692
                     /locus_tag="OO172_004297"
                     /pseudo
     CDS             595..692
                     /locus_tag="OO172_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          175 a          167 c          209 g          141 t
ORIGIN      
        1 gccagccccg tcccgcccga tagagcgcgg catagcgggc cccggattac ttgcgtgcga
       61 cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg
      121 gattaccaga gtcagagtgt cttgcagtca ggcagcagcg aagcgaagcg ttactgacgt
      181 cgctgcatga atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg
      241 aagcattcag ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggtc
      301 tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga
      361 aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctgtacgggc
      421 tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga
      481 gcgtactgcc ggaatggcct tccaaccgag ttgacgacct cctgccatgg aaggtggtgc
      541 taccatcagg ataatcgtca atacggtgtt cggtgaacgt ttacttggag catcatgctt
      601 catgaagaaa aactggcacg tcatcaacgt aaacaggcga tgtatacccg aatggcagcc
      661 ttcccggcgg tgaaaatgtt cgaagagtat ga
//
LOCUS       DAJTNM010000320          692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTNM010000320 DAJTNM010000000
VERSION     DAJTNM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.327"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>692
                     /gene="ydfJ"
                     /locus_tag="OO172_004298"
     CDS             <1..>692
                     /gene="ydfJ"
                     /locus_tag="OO172_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5514187.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCA"
BASE COUNT          157 a          151 c          182 g          202 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa tgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cg
//
LOCUS       DAJTNM010000321          691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTNM010000321 DAJTNM010000000
VERSION     DAJTNM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.328"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..384
                     /locus_tag="OO172_004299"
     CDS             <1..384
                     /locus_tag="OO172_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5514188.1"
                     /translation="IEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHL
                     FIMMAAVVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKG
                     YIYFAMFFSIAVESLNLIRNKKNPL"
     gene            522..>691
                     /gene="ygdR"
                     /locus_tag="OO172_004300"
     CDS             522..>691
                     /gene="ygdR"
                     /locus_tag="OO172_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5514189.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQG"
BASE COUNT          166 a          152 c          170 g          203 t
ORIGIN      
        1 attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg
       61 cagattatgc tgctggatat tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg
      121 tcagatcacc tgtttattat gatggcagcc gtggtgattg ccgtaggcgt gatgatgttc
      181 gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat gctggcgctc
      241 tctttcctga ttctggtggg ctttaccctg attctggaaa gtttcgacat ccacgtaccg
      301 aaaggttaca tctacttcgc gatgttcttc tctattgcgg ttgaaagcct caacttgatt
      361 cgcaacaaaa agaatccgct ctgatactcc gtacgctctc ctgcgggagg gcgtactcac
      421 tccttttacc ctcacagttt aagattttcc tgctttcaag atatataacg tcgatttata
      481 aacagactat tatcataggt aggccaaatg aggataaccg gatgaaaaaa tgggcagtaa
      541 taatttctgc agtcggactg gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg
      601 cgaccaaaga tggccgtatg attttgaccg atggaaaacc tgaaattgat gatgataccg
      661 ggctggtgag ttatcacgat cagcaaggta a
//
LOCUS       DAJTNM010000322          683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTNM010000322 DAJTNM010000000
VERSION     DAJTNM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.329"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            166..>683
                     /locus_tag="OO172_004301"
     CDS             166..>683
                     /locus_tag="OO172_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514190.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGAEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTV"
BASE COUNT          165 a          189 c          163 g          166 t
ORIGIN      
        1 cgctactcat tccgctggtc gctcacgtgc cgctatcgtc gctgaccttg agtcaggcgg
       61 taactggatg gacagcttgt aagcaagcgt ccctcatctg agaacacacg gtccctgact
      121 gcctgttagg gacttcaagt atttgactca aggagattaa ttataatgaa tatgactaaa
      181 aacgctttcc agtttggcat tgagcctgtt catatcaccg acaacgacaa ccttcaggtt
      241 aatgagggcc ttccgactaa cgccgaccct caggtctatg ccctccagtt agctaagact
      301 gttaaggcta tgctgaacgg tgtacttaaa gacgctcaag ataacatccc gttccctgta
      361 gaagtcctgc cgacccgtaa cagcttaccg actccaatca ttgctcacac tctggctgac
      421 cgctctgtgc tggtccctgt ccgtggtggt aagcgacctg aagttgtgac cgctccgtct
      481 ggcgctgaaa tcaccgttga gccgattgaa caggcgatcc tcgtgtctca ccaaacaaag
      541 ctttgggacc agaagtcgac cactggtttc actcagggga cgctccagca ggacgcactg
      601 aacatctgcg ataacgtgat tcgtaccatt aactctaaga tggttgacgt gctggagtcc
      661 tcaaagctgc tgaagaccgt aga
//
LOCUS       DAJTNM010000323          676 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTNM010000323 DAJTNM010000000
VERSION     DAJTNM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.330"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..239)
                     /locus_tag="OO172_004302"
     CDS             complement(<1..239)
                     /locus_tag="OO172_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiP family protein"
                     /protein_id="HCR5514191.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLFHSTWNANLDFQS"
BASE COUNT          202 a          131 c          159 g          184 t
ORIGIN      
        1 gactgaaaat cgaggttggc attccaggtg gagtgaaaaa ggttggtttt gtatttgtcg
       61 ccgtcggtaa catctttacg gtcgcgttca cgctgccagt aatagatacc gccggttaag
      121 gttgaatcgt cgatgaatcc ttcggcgcga gcctccggca ttgccataaa gcccgacatt
      181 gctgtaacac cggcaatagc cagcgccagc gtactacgtt tgccactaaa cgtacgcatg
      241 ggttaatcct ctttgacgta taaattgctg caccaaaggt gaacagcgaa aaaaaataaa
      301 aattaaaaaa gtagcgttga gaaaagtctc gctgactaca ggaacagact atttttcgcg
      361 acgcgaatta tcaatctctt tctgtaacca gaatgtaaga acatgagcaa ccgcacatat
      421 tttgatgatt tttgttacaa ggcttaagtt tgcggttgaa aggcttgcag aagaataaaa
      481 acgtgaaatt ccagcaagat gaaagcggtt acattttatt taattatgtt ttgtgatttt
      541 aaatttattt tatggataaa ttgcgaagcg gaattaattc gcgaagaaaa gcagaaaaaa
      601 cgccgcagag cgcggcgttt gggattgttt gctatttctt gccggatgcg gcgtgaacgc
      661 cttatccggc ctacaa
//
LOCUS       DAJTNM010000324          674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTNM010000324 DAJTNM010000000
VERSION     DAJTNM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.331"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            17..169
                     /locus_tag="OO172_004303"
     CDS             17..169
                     /locus_tag="OO172_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514192.1"
                     /translation="MGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEI
                     FQKGAA"
     gene            166..>674
                     /locus_tag="OO172_004304"
     CDS             166..>674
                     /locus_tag="OO172_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR5514193.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDII"
BASE COUNT          148 a          161 c          201 g          164 t
ORIGIN      
        1 tgacgcgatt aactggctgg gggactggtg cagtgaagag cagcatccgg tgatctggtc
       61 aaccacgcag gctgcagaac acttaccggt tcgcatgccc cgtctctgct ctgcagaacg
      121 cctctctgaa agtatggtcg atgaaatatt tcagaagggg gctgcatgag tatagaaata
      181 aaaacagggc tggtgctgtc cggcggtggg gcggtgggcg cttatcaggc gggagtggtt
      241 aaggcactgg cagagtgtgg tacacagatc agcatggttt caggggccag cattggcgca
      301 ttcaatggtg ccattatcgc ggcctctccc gatctgtcag aagctgccgt acgcctggag
      361 gcgctctggg atcatcttgg gaataatcag gtgctgtcgg taaaccgatc ggtttacttt
      421 tcattgctga aaaaattgtt ccaggcaatg aacctctgcc agatccccgg acgtgcagga
      481 gcactgctta cgacgcttct tcgccatata tcgatactca acgggtttga caatctgatg
      541 gctcagccgt tgttgtcaga tgagcccctg acagcgctgt tggatcatta tcttgatact
      601 gatgctctgg cagacgggct accgctgtat gtgtcgctgt accccacaga agggggcatg
      661 caggatatta ttga
//
LOCUS       DAJTNM010000325          668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTNM010000325 DAJTNM010000000
VERSION     DAJTNM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.332"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..577)
                     /locus_tag="OO172_004305"
     CDS             complement(<1..577)
                     /locus_tag="OO172_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5514194.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVISNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHGAEDVISPMELRLFNYLVERKDLI"
BASE COUNT          181 a          157 c          163 g          167 t
ORIGIN      
        1 gaatcagatc tttacgctcg accagatagt tgaacagacg caattccatc ggtgaaatca
       61 catcttcggc gccatgcaga ataaaaccag caaaagcgaa attggcgcta cgttcaaact
      121 gggtgatggc gtccagcacg ttgttcagac agtcggcttt gctggtggga ccaggacgcg
      181 cgcagactac cttatgcacg ttcggaaagc gagcgcacac ttcgtcaaca tcacgttgag
      241 tatcggggtc gttggggtag gtgccaacaa agatatgata gttttcgtag tcgagcgtgg
      301 tcgccgccag ctcggccata ttgctgatga cgcccgtttc attccacgcc ggaaccataa
      361 tcgctaacgg tttttcatct ggtttataca gttcgcggta actcattcgc gggtagcggc
      421 gataaacact caacttgcgt ttaatgcggc gtacccagta gacgacatca ataaaaaaat
      481 cgtccagccc gctgatgaac atgatgaccg ctaacgttat cgcgattact tttaagccgt
      541 agagccaggt agcaaaaaca tcaagaagcc agtccacaca aaaaccttac attaacgctg
      601 gttatgttta gggtggcgta tattaaggtt ttttatgaat tgtgacagct ttttaccatt
      661 aatagggt
//
LOCUS       DAJTNM010000326          663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTNM010000326 DAJTNM010000000
VERSION     DAJTNM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.333"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>663
                     /locus_tag="OO172_004306"
     rRNA            <1..>663
                     /locus_tag="OO172_004306"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          154 a          146 c          222 g          141 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ccctttaagg gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
      661 agg
//
LOCUS       DAJTNM010000327          657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTNM010000327 DAJTNM010000000
VERSION     DAJTNM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.334"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..282
                     /locus_tag="OO172_004307"
     CDS             1..282
                     /locus_tag="OO172_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR5514195.1"
                     /translation="MFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFESLCI
                     DHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            288..>657
                     /locus_tag="OO172_004308"
     CDS             288..>657
                     /locus_tag="OO172_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5514196.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNR"
BASE COUNT          190 a          151 c          164 g          152 t
ORIGIN      
        1 atttttgatc gtattgaaga aatggcatgg gctcgtcact accagcagat cgttcgtgaa
       61 gaaaaagaaa cggaactggc ggacgaccta gaaaaaggtc tgcctcagca cctgtttgaa
      121 tcgctctgca tcgaccattt gcaacgccac ggtgccaata aacaggcaat cagccacgca
      181 tttgatgatg atgttgagtt tcaggagcgc atggcagaac acatccggta catggctgaa
      241 accattgccc accaccaggt tgatattgat ttggaggact aaaatctatg gcgctttttc
      301 aacgagcaat aaacacacag gcctatctaa aagcaggaat tatggggttt gctggcgacg
      361 gcaaaaccta cacagcaagc gaactggcta ttggtctggt aatgcttatg cggcaacggg
      421 gaattgaagc tggtaaccgc ccggtaatgt ttctggacac agaaacaggc tctgactggg
      481 ttaaaccccg ctttgatgcg gaaaatatcg aattatttac ggcaaaaacc cgctcgtttg
      541 ttgatctgct tgaagccatt aatgaagcgg aaagcagcgg ttccgtaatg atcatcgact
      601 ccatcagtca cttctggacc ggattatgcg acgagtatgc cagacgccga aaccgta
//
LOCUS       DAJTNM010000328          648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTNM010000328 DAJTNM010000000
VERSION     DAJTNM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.335"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..648
                     /locus_tag="OO172_004309"
                     /pseudo
     CDS             1..648
                     /locus_tag="OO172_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          160 a          170 c          152 g          166 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatatcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcattt
//
LOCUS       DAJTNM010000329          645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTNM010000329 DAJTNM010000000
VERSION     DAJTNM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.336"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>645
                     /locus_tag="OO172_004310"
     CDS             <1..>645
                     /locus_tag="OO172_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCR5514197.1"
                     /translation="LLRQMVDEPDVLSDGKNENGQLFDKQKALKRIKELEGEQIKTAR
                     REMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMTSVPTLIRHVPGKTEPVLHLEHI
                     QPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELLDILTDDGWLKNEYHGEEGIFTG
                     LASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEFSHLVGMDECQGTLTLVDTP"
BASE COUNT          178 a          161 c          177 g          129 t
ORIGIN      
        1 tctgctccgg caaatggttg atgagccgga tgtgttatct gacggtaaga acgaaaacgg
       61 gcagcttttt gataaacaga aagcactgaa aagaattaag gagctggagg gcgaacaaat
      121 caaaaccgcc cgcagggaga tggtgctggc tgttgtcggc acgatgaaag caggcaaatc
      181 aaccaccatc aacgccattg tggggcagga aattctgcct aaccgtaacc gccccatgac
      241 ctctgtaccg acgctcatcc gccacgttcc cggaaaaact gagccggttc tccatctgga
      301 acatattcag cctgtccgca atttattaat cacactgcag gaaaaactcg ccaccccggc
      361 aggacagcag gtcgcacaga ccctgcagca aaccggggat acccgcgaac tgctggatat
      421 tctgacggat gatggctggc tcaaaaatga ataccacggg gaggagggaa tctttaccgg
      481 actggcatcg ttaaacgatc tggttcgtct tgctgcggca atggggagtg aatttccttt
      541 tgatgaatac gcagaagtgc agaaactgcc ggtgatcgac gtggaattca gccatctggt
      601 ggggatggat gaatgccagg gaacactcac actggtggat acccc
//
LOCUS       DAJTNM010000330          644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTNM010000330 DAJTNM010000000
VERSION     DAJTNM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.337"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            167..503
                     /locus_tag="OO172_004311"
                     /pseudo
     CDS             167..503
                     /locus_tag="OO172_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          210 a          109 c          132 g          193 t
ORIGIN      
        1 ttccaattaa agccgttatt ataataatta tcatatatgg ttattttcat gttgatttcg
       61 tcagaaatag aaaatgctga tgatgaattg tgaataatca aactgacctc taattatcct
      121 gtatggagta gtgcttattt tcgactcaat ttctactgat tagattatgg ttatcagtac
      181 agtgggttat gaaaaacgga ctggatctgc cagcagtgct tctattatta ttcaaaaaga
      241 acttcagccg gacaatgcta taatccaggg aaaggctata agctctgttg gacggaatgt
      301 ggaaatcagc actggtaaaa aggggaactg acattaacac aagaaggatt cctgcaagca
      361 actgcttatc tgcccggaac gcagaattaa agcataacca cacaggagcc agaatactgt
      421 ggtcgtacgc attcagtatt gtgacaacta atagaaataa aaatctggcc ggtgcgaata
      481 ttaaacgatg cgataccgta tagtgtaagt caaaagtaac ggggtattta aaccatcttt
      541 tcatatttat ttaacataat gcagattctg atgtattagt tcagttgttc gccagaatca
      601 gcgctcgatc actatgcgtt ttagcagtgt gctgaagaag ttct
//
LOCUS       DAJTNM010000331          633 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTNM010000331 DAJTNM010000000
VERSION     DAJTNM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.338"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..393)
                     /locus_tag="OO172_004312"
     CDS             complement(<1..393)
                     /locus_tag="OO172_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5514198.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEV"
BASE COUNT          224 a          146 c          108 g          155 t
ORIGIN      
        1 gacttcatca atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc
       61 accaccaagc agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc
      121 catatcaatg gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt
      181 ataaaagaat tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca
      241 aagatgatct ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc
      301 taatccacaa agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca
      361 agtaacaaaa acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag
      421 acgtttcaaa cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac
      481 cgtgatcaca ttaaaaacaa aaatttccac cgaggagaaa aaataccctc aaaaaccacc
      541 tgaataatta accattattg ttaaaacaac acgttaaata atcacataac aaactccatt
      601 aatcattgaa aatgagcaca gaaatagaaa tta
//
LOCUS       DAJTNM010000332          619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTNM010000332 DAJTNM010000000
VERSION     DAJTNM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.339"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(14..>619)
                     /gene="ospZ"
                     /locus_tag="OO172_004313"
     CDS             complement(14..>619)
                     /gene="ospZ"
                     /locus_tag="OO172_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR5514199.1"
                     /translation="VEHGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYM
                     KTMYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEI
                     SCKIFSKENSDFLYIIVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIK
                     SEIIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          188 a          125 c           91 g          215 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaactctgg cgactcatct cttaacactt
      181 gcataatctc tctttttata tcatatagtt ctataaaccg atttgcccgt agttctcctt
      241 ggggagaatc atttctgaat acaacaatta tatacaaaaa atcactattt tctttagaga
      301 agatcttaca actaatctct gggagctcac ataatgcatg taagtcagga tggtacaaac
      361 tatccacaga aaattgcccc ataggacgtt ttccagatat aactgtcaac cagcttcctt
      421 ccggtgtact ggcttcgtct ctggaaatag gctgaaaatc ttccggacat ggagaataca
      481 ttgtcttcat ataattaagt cgtctggatt tctcattaat aaactcctct atttttaatt
      541 gtgccatact tctggttata ccagagatat acttcttgtc atatatgttt ggtgattcat
      601 taaagtaacc atgttcaac
//
LOCUS       DAJTNM010000333          615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTNM010000333 DAJTNM010000000
VERSION     DAJTNM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.340"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..521
                     /gene="ospC2"
                     /locus_tag="OO172_004314"
     CDS             <1..521
                     /gene="ospC2"
                     /locus_tag="OO172_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5514200.1"
                     /translation="VSLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASN
                     YEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSKFNFTKQDVAEMEKMNNNMYCEL
                     YDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLL
                     KNGAVSGKRFGR"
BASE COUNT          223 a           83 c          121 g          188 t
ORIGIN      
        1 ctgttagtct ggatagaatt ataaactttg tgtttcagct tgagtatcat ataccaagaa
       61 tgctaagcac agataacttc aaaaagataa gactcagaga tatatctttg gaggatgcca
      121 ttaaagcatc caattatgaa gaaattaaca ataaggtgac tgataaaaaa atggctcatc
      181 aagctcttgc ttattctcta ggcaatgcaa aatctgatat ggcactttat ttactatcta
      241 aatttaattt tacaaaacaa gatgtcgcag agatggagaa aatgaacaac aatatgtatt
      301 gtgagctgta tgatgttgag tatttactta gtgaagatag tgccaactat aaagtgctag
      361 aatattttat taataatgga ttggttgatg taaacaaaag atttcaaaaa gcaaatagtg
      421 gggacactat gcttgacaat gcaatgaaaa gcaaagattc aaaaacaatt gattttttat
      481 taaaaaatgg agcggtatca ggcaaacgat ttgggaggtg acctaaaata tatttaggtt
      541 gtgccctgaa tctgacgttg tcatttttgt tatattgatc gatatagggg atggtgcaga
      601 ccgttcccct gcaac
//
LOCUS       DAJTNM010000334          614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTNM010000334 DAJTNM010000000
VERSION     DAJTNM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.341"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
BASE COUNT          138 a          174 c          184 g          118 t
ORIGIN      
        1 cagcgccttg agcgcaaccg gaaaccgtct ggggagaacc ccagaccccc atcgctgcaa
       61 catcgacggt ctgcaccacc tgccaaccga gtaactgcct gttccgtgcg aatgggcttt
      121 gcagggggct ggcgcggttc ctggcgacct gaaagcccct ggttaggggt atcggcgcgc
      181 ctgacctcct gagagcctct gtaacgcatt ttcgcgcctc ccatgccctg gcgggcattt
      241 aaaacccgtt tcgtgcgtct gagagcgttt gaagggctat ctgacgactc atccccgaaa
      301 gaacaacgac ggaatcaccg acaacatgcg agaggtggca ccgctcgccg cagccccagc
      361 gaccgaacgt tagtgagcga gtgggcgagg aagcggaaga gaaccggtag ttagattgag
      421 cacgtacgca ctgatgggtg tcggggcgca gccctgacca gatggcaaac ggaacatcgt
      481 cgtgggtgac ggtattacat ttgcacatcc tgtcccgatt tttgaggcat tttagccttc
      541 atcccccttt tagtggtgaa cggacggcaa aaacatgatg gaactgttga acgtggagag
      601 cgcgaaatcg gaca
//
LOCUS       DAJTNM010000335          611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTNM010000335 DAJTNM010000000
VERSION     DAJTNM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.342"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
BASE COUNT          221 a          108 c          127 g          155 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catattaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg ggggggatga aaatacctga agcagtaaat catattaatg
      541 ttcagaataa tattgatctt gttgatggaa aaacgaatcc gaataaagca acaaaagcat
      601 tacaaaaaaa t
//
LOCUS       DAJTNM010000336          591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTNM010000336 DAJTNM010000000
VERSION     DAJTNM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.343"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(285..>591)
                     /locus_tag="OO172_004315"
     CDS             complement(285..>591)
                     /locus_tag="OO172_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5514201.1"
                     /translation="DIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGAN
                     YKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          174 a          123 c          100 g          194 t
ORIGIN      
        1 aggctacctc actcccgtaa agacaggagt caagttcccg ctccgacata actgcgtgca
       61 atggattttg aaggaaccgt cagcccattc cccggaggca aacatgggct ggcaaaagtg
      121 tagcggtatt aggcacagct atttaggaaa gagggagcat ctccatctca ttaaatggac
      181 cacgccagac aattcactgt catgctgatg ctgcactgtg gggacagaat cactcatgat
      241 gacttcgata atcgacgaca ttattatttg gccgagcttt taggtcatat ctcaaatcgt
      301 ttgcctaata ttgctccatt ttttaataaa aaatcaatca tttttgaatc tttgcttttc
      361 attgcattat caagcatagt gtccccacta tttgcttttt gaaatttttt gtttacatca
      421 accaatccat tattgataaa atattctagc actttatagt tggcaccatc tttactaagt
      481 aagtattcaa catcatacag attacaatat ctattgttgt tcattttctc catctctgcg
      541 acatcttgtt ttgtaaaatt aaatttagat agtaaataaa gtgctatatc a
//
LOCUS       DAJTNM010000337          589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTNM010000337 DAJTNM010000000
VERSION     DAJTNM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.344"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            31..318
                     /locus_tag="OO172_004316"
     CDS             31..318
                     /locus_tag="OO172_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR5514202.1"
                     /translation="MFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNT
                     VSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            321..>589
                     /locus_tag="OO172_004317"
     CDS             321..>589
                     /locus_tag="OO172_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5514203.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITA"
BASE COUNT          180 a          125 c          151 g          133 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
      361 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      421 gaaaaaactg gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca
      481 ccggagatgg tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg
      541 ccagcgttat cgaagttccg gatattaccg ctaaccgacg gattaccgc
//
LOCUS       DAJTNM010000338          571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTNM010000338 DAJTNM010000000
VERSION     DAJTNM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.345"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..452
                     /locus_tag="OO172_004318"
     CDS             <1..452
                     /locus_tag="OO172_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5514204.1"
                     /translation="EAEQQGKNEEQRQHDEWVAERNREIQQEKQRRANAQAAANKRAA
                     TAAANKKARQDKLDAEATADKKRDQSYEDELRSLEIQKQKLALAKEEARVKRENEFID
                     QELKHKAAQTDVVQSEADANRNMTEGGRDLMKSVGKAEENKSDSWFN"
BASE COUNT          205 a          119 c          155 g           92 t
ORIGIN      
        1 cagaagcgga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa tgggtcgcgg
       61 agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag gccgccgcga
      121 acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa ctggacgccg
      181 aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc agcttagaga
      241 ttcagaaaca aaaactggcg ctggcgaaag aagaagcccg cgttaagcgc gaaaacgaat
      301 ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa tctgaagctg
      361 acgcaaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg ggcaaagcag
      421 aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca agcctatgat
      481 tcttgaagat aaaaaaccct ctgtagtaac ggagggtttt gttcattcat agtgcaggga
      541 tcaaaatcat tcccactcaa tggtagctgg c
//
LOCUS       DAJTNM010000339          550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTNM010000339 DAJTNM010000000
VERSION     DAJTNM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.346"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            20..496
                     /locus_tag="OO172_004319"
     CDS             20..496
                     /locus_tag="OO172_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5514205.1"
                     /translation="MFIDSEKRLKQLSDEAKKSTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          175 a          110 c          134 g          131 t
ORIGIN      
        1 attttcagag ggaaaaatca tgtttattga ttcagaaaaa cggctgaaac aactttcaga
       61 tgaggcaaag aaaagcaccg aggatctcga agaagcaaag aagaattcaa ggtttacaca
      121 ggtatcccca aaaggttggg aacgcgttcg agagctgctg aaggatagcc aaggcatatc
      181 agcactgaag ctgtactcat ttttagcaga gcatatcgat cctacgtgtg gcgctgtcgt
      241 tgcggatcag caattcctag ctgaaaaact tggagttagc agaagcacaa ttattcggtg
      301 gctcaattac ttagaatcaa aaaatgcatt agttagaatc cccgttgctg gtaaggtttg
      361 tgcgtatgcc ctcgatccac atgaagtctg gaagggatac aacactacga aaaaccatgc
      421 agcgtttgtc actaaaacac tggtcaacaa agacggtgat attcagcgcc gaatcatggc
      481 catgttttca aattgagcca gcggcaggcg gacaatcagg ggctacgtgt taacgttttg
      541 accatgattg
//
LOCUS       DAJTNM010000340          550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTNM010000340 DAJTNM010000000
VERSION     DAJTNM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.347"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..412)
                     /locus_tag="OO172_004320"
     CDS             complement(<1..412)
                     /locus_tag="OO172_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5514206.1"
                     /translation="MWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTP
                     PEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVR
                     ESNGEEQHFSVPFQTPAIALHEGYFKYSVMGGNIK"
BASE COUNT          142 a          157 c          107 g          144 t
ORIGIN      
        1 tctttatatt cccccccatc acactatatt tgaaatatcc ttcatgcaga gcgatggccg
       61 gcgtttgaaa tggcacggaa aaatgctgct cctcgccatt actctctctc actgtaacat
      121 caagctcccc gccaacggcc agcgttggta aattatccag cgcaaaaggc cctggcggca
      181 cagaacgggt aaaaataatt tgcccatttt gtttaataat aacctgcgcc tcactttgtg
      241 ctattccatg caccacaggt gtgtagtagc tgtcctccgg cggagtcatc gcatcgtcgg
      301 tcgctaacat cccgccgcga aaaggtatgc tgtcaaacac ctcagacgac gtataagatt
      361 ctccaagcgt taatcggctt ttaatcgcat ttatgcctcg ctcaaaccac atgtaacccc
      421 tttgccatct ttctgactgg ccaagttcct tgctccatgt cactgaacta cgaaacctcc
      481 aggcaccaag attaaaccct ggagtgaacc tgccataacg agaattacgc gtggtagatt
      541 gattatcatt
//
LOCUS       DAJTNM010000341          549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTNM010000341 DAJTNM010000000
VERSION     DAJTNM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.348"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>549
                     /locus_tag="OO172_004321"
     CDS             <1..>549
                     /locus_tag="OO172_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5514207.1"
                     /translation="DDWLKKEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLM
                     AVMGLAGVLRGKKVRTTVSRKTVATGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYV
                     AFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTE
                     RLKEAGLLASTGSTGDSYDNAMA"
BASE COUNT          123 a          127 c          174 g          125 t
ORIGIN      
        1 cgatgactgg ctgaagaaag agatacagcg cgtatacgat gaaaatcatc aggtgtacgg
       61 tgtgcgtaaa gtctggcgtc agttgttacg ggaaggaatc agggtggcca gatgtacagt
      121 ggcgcgcctc atggcggtta tgggacttgc cggtgttctc cggggtaaaa aggtccgcac
      181 taccgtcagc cggaaaaccg ttgccacagg tgaccgcgta aaccgtcagt tcgtggcaga
      241 acgtcctgac cagctgtggg tggctgattt tacttacgtc agcacatggc agggcttcgt
      301 ctatgtggcg tttatcattg atgtgtttgc cggatacatc gtggggtggc gggtctcatc
      361 gtctatggaa acgacattcg tgctggatgc gctggagcag gcgttgtggg cccgtcgtcc
      421 gtctggcacc atccatcaca gcgataaagg ctctcagtat gtgtcactgg cctatacgga
      481 gcgactaaaa gaagccggat tactggcatc aacagggagt acaggcgact cgtatgacaa
      541 cgcgatggc
//
LOCUS       DAJTNM010000342          548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTNM010000342 DAJTNM010000000
VERSION     DAJTNM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.349"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..271)
                     /locus_tag="OO172_004322"
     CDS             complement(<1..271)
                     /locus_tag="OO172_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5514208.1"
                     /translation="MEYRTWITEALRLHFEEYLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKR"
BASE COUNT          140 a          117 c          152 g          139 t
ORIGIN      
        1 gacgtttttt taccaccggg agtttgggca taacggtgct ttcagtaagt acgacaggta
       61 ctgtggaggc acttccgtaa agaagggcat caagttcctg ctccgacata cctgcgggca
      121 gaggccatga aaggccagcg ttgcggaagc gcacgaacat accacagaca gttgattttg
      181 gtacgcccag gcgacgtccg gctacaaccc gtggtaaata ttcttcgaaa tgaaggcgta
      241 aagcttcagt gatccaggtc cggtattcca tacgatagtg tccacttcaa aatgatggac
      301 attatttttg tggagccgga ggaaacagac cagacggttt aaatgagccg gttacgaaga
      361 agctgaggtt ctgcgctttg ataattttga cgatgccgtt gagtgttgcg cagacctgaa
      421 tattccgttc tttgtggatg ccagaaacaa aaaactggtc ttctggtttg tacgtgttga
      481 tgacgaaggg tatccggaaa ttgcccgctg catggagcgg gaatttgtga caattcctgc
      541 cggtatca
//
LOCUS       DAJTNM010000343          545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTNM010000343 DAJTNM010000000
VERSION     DAJTNM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.350"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..>545)
                     /locus_tag="OO172_004323"
     CDS             complement(<1..>545)
                     /locus_tag="OO172_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514209.1"
                     /translation="LGVPGMPATAKGIRQALQRFSRALGDVSRRRAGTKAIEYHIDCL
                     PEVTQKALRERYVEQLVATENNVSEVKTVTRTARNPDAVQAVEAYRGSPQLMEERLNA
                     LTENQRRVSEARAALAVEVLKLESAGNLGRLKAINFLVEKARKGELPERLQQAAINAN
                     AKRGTNRTISRDPLYQWVLKY"
BASE COUNT          110 a          154 c          137 g          144 t
ORIGIN      
        1 ttgtatttca gtacccactg gtaaagcgga tcacggctga tggtgcgatt agtgccacgt
       61 tttgcattag cgttaatcgc agcctgttgc agtcgttccg gcaactcacc tttacgggct
      121 ttttcaacca gaaagttaat ggctttcagt cgcccgaggt taccggcgct ttccagcttc
      181 agcacttcca ctgccagcgc cgctcttgct tcagacaccc ggcgctgatt ttcagtcagg
      241 gcattcaggc gttcttccat tagttgcgga gaaccccgat atgcttcaac tgcctgaaca
      301 gcatcagggt tacgcgccgt cctggtaacg gtcttcactt cggagacgtt gttctctgta
      361 gccaccagtt gttccacata gcgttcacgc agtgcctttt gggttacctc aggcaggcaa
      421 tcgatatggt actcaatggc ttttgtacct gctcgacggc gactaacgtc accaagagca
      481 cgagaaaaac gctgtaacgc ctgtcggatg cctttcgcgg tggctggcat tccaggaaca
      541 ccgag
//
LOCUS       DAJTNM010000344          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTNM010000344 DAJTNM010000000
VERSION     DAJTNM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.351"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(335..>525)
                     /locus_tag="OO172_004324"
     CDS             complement(335..>525)
                     /locus_tag="OO172_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5514210.1"
                     /translation="QSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINLLDKSDYVL
                     AHGLNTDDQLAVGIVYQF"
BASE COUNT          143 a           81 c          138 g          163 t
ORIGIN      
        1 tgtggaataa ttgtggtact gatgattatt tatatcccgg attggtaggg aaaagctttt
       61 ttcatgcact atttttgcaa atatttctat atgggaagcg gttttgttac agattaaatt
      121 tataaagaaa tgcatttggg gtgttgggtt gaagagtagc tggtagatgt tattgtgcca
      181 gcaggggcag gggaatgtgc cgtatcgata tgctttggca attgcgtgtg ccgcagatgg
      241 agatgaaatg ccaataaaaa agcctgagtt cgatgaggga cgaactcagg ctggtgttat
      301 gttttacagt aacacgcctc ggcgcgttgg ctgattagaa ctggtagacg ataccaactg
      361 ccagttggtc gtcggtgttc agtccatgag cgagaacata atcactctta tcaagcaggt
      421 tgatctgata agcggtatat atgttcatat tcttattgaa gtaataccag gtaccgattt
      481 caatatagtt cagacgatca gaatctttgt aacctgtaga ctggc
//
LOCUS       DAJTNM010000345          522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTNM010000345 DAJTNM010000000
VERSION     DAJTNM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.352"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
BASE COUNT          119 a          128 c          143 g          132 t
ORIGIN      
        1 tacccgatta gttgttaatc atatttaatc atagtgatta ctttcagacg gaacaaggca
       61 aataatgcga gtcagcgcat ataaggcggc atacggtggt tacatgtagc acaggtgtaa
      121 aaccgccgaa gcccggcgca agccggtacg gtcgtccgaa tatcaccagt ccccctccgg
      181 ctctgcgagg ccatgcggcc tgtcagcgat gaaaatgcct ctgtgcgcgg tcggagtggt
      241 cccgacaagg gtttacccgc agtcggggcg tatctccgcg ttagcgggcc gtgagggccg
      301 cttacgagcg tgtatgagag acgtacagta gttttgtccg ctcacaccag tacaaaccag
      361 tcgaaatacc ggtaacgacg attactcttg tctctgatgc cggtggacta cgagatttta
      421 tttactggcg tcctgatgcg gtcaggactg gtatggaacc tgtttatgtg atacttaatg
      481 ctcttctgga ttccgggcga tttacgcgta aacttattct gt
//
LOCUS       DAJTNM010000346          512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTNM010000346 DAJTNM010000000
VERSION     DAJTNM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.353"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>512
                     /locus_tag="OO172_004325"
     CDS             <1..>512
                     /locus_tag="OO172_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5514211.1"
                     /translation="YLEPETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQ
                     NWKPATLSENRASHSTLIEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSM
                     PLSDLLNRESKTDCLDVVTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKA
                     SDARDAFSPE"
BASE COUNT          133 a          138 c          127 g          114 t
ORIGIN      
        1 taccttgagc ctgagactgg cagtctaaga ccgtccgtct ccctatctgt attggcggaa
       61 cctgaagtcc ctgaacctaa agcactgacc ctcgcgtctc tcattgagca gtacgagcag
      121 gagaacgccc agaactggaa gccagcgacc ctaagcgaaa accgagcgtc acactctacg
      181 ctaatcgaga tattcgacta tctggatatt caggatgtcg gcaaggcgac ccgtgcagat
      241 atgctcagag ttcgtgaagt cctccagcag ctaccgaaga accgcaagca acgctttaag
      301 tctatgccgc tgtctgactt gctgaaccgg gagtctaaga ctgactgtct ggacgtcgtt
      361 accattaaca ataaatatct tatcaagatg gctgctgtgt ttaagtgggc ggtacgcaac
      421 gacttgatag ctaagaacct gaccgaaggt cttgagctga aagtccctca gcgtaaagcc
      481 tccgacgctc gtgatgcgtt cagtcctgag ca
//
LOCUS       DAJTNM010000347          505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTNM010000347 DAJTNM010000000
VERSION     DAJTNM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.354"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..505
                     /locus_tag="OO172_004326"
                     /pseudo
     CDS             1..505
                     /locus_tag="OO172_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          147 a          120 c          137 g          101 t
ORIGIN      
        1 gaagctcgcc ttgagcgaga tatattaaaa aaagcaacag cgtattttgc acaggagtcg
       61 ctgaaaaata cgcgttaatc gaacaatggc gacaacaatt tcccattgaa gcgatgtgtc
      121 aggtatttgg tgtatccagg agcggttatt acaactgggt acagcatgaa ccctcagaca
      181 gaaaacaaag tgatgagcgg ctaaaactgg agattaaggt ggcacatatc cgcactcgcg
      241 aaacatatgg aacccggcgg ctccagacgg agctggcaga gaatggcatc atcgttggtc
      301 gtgaccgact ggcacgtctt cgtaaggagc tgaggctacg ctgtaagcag aaacgcaagt
      361 tcagagcgac tacgaacccg aaccacaatc tgccagttgc gccaaatctg ctgaaccaga
      421 cgttcgctcc tacagcacca aatcaggtct gggtggcgga cctgacgtat gttgccacac
      481 aggagggatg gttgtacctc gctgg
//
LOCUS       DAJTNM010000348          494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTNM010000348 DAJTNM010000000
VERSION     DAJTNM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.355"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..>494)
                     /gene="chbR"
                     /locus_tag="OO172_004327"
     CDS             complement(<1..>494)
                     /gene="chbR"
                     /locus_tag="OO172_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR5514212.1"
                     /translation="NGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQ
                     HYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHY
                     REEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPM
                     QIIN"
BASE COUNT          149 a          122 c           89 g          134 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgtt
//
LOCUS       DAJTNM010000349          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTNM010000349 DAJTNM010000000
VERSION     DAJTNM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.356"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..>489)
                     /locus_tag="OO172_004328"
     CDS             complement(<1..>489)
                     /locus_tag="OO172_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5514213.1"
                     /translation="ENGYRFLLLLLQGESDKQHIINEVWREQKGSVSDSSYYGQIYML
                     RKALDVAGLSSSLIKTIPRKGVRYLGKVEKHVCENERDSDEDSSSYTNDSEAREVNTD
                     ANIPENDNSQEFSTEELLPIKHTDKNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVL
                     LFL"
BASE COUNT          132 a          120 c           85 g          152 t
ORIGIN      
        1 agaaatagta agactaaaaa aacaagcgtc gtcagccagc acactgctaa cacggtaaga
       61 acgctaataa aaacactcca ctgcttcgtt ctataccatt caatattttt gtcagtatgt
      121 tttattggga gcagctcttc agtagagaat tcctggctgt tatcattctc aggaatgttt
      181 gcatcagtgt ttacttccct ggcctcgcta tcgttggtat agctactact gtcttcatca
      241 ctgtcacgct cattctcaca gacatgtttc tcgacctttc ccagatatct gacgcctttt
      301 cgcggaatgg ttttaattag agaactcgac aaacctgcga cgtctaatgc ctttctaagc
      361 atgtaaatct gcccataata actgctgtcg cttaccgaac ctttctgctc ccgccaaacc
      421 tcattgataa tatgttgctt atcggattct ccctgtagta aaaggagtaa aaatcgatag
      481 ccgttttca
//
LOCUS       DAJTNM010000350          484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTNM010000350 DAJTNM010000000
VERSION     DAJTNM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.357"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..140)
                     /locus_tag="OO172_004329"
     CDS             complement(<1..140)
                     /locus_tag="OO172_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5514214.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QS"
     gene            158..310
                     /locus_tag="OO172_004330"
     CDS             158..310
                     /locus_tag="OO172_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514215.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT          152 a           79 c          107 g          146 t
ORIGIN      
        1 ttgctctgat ggtgcagcca ggcaccgacg gacgagtaga taaaacggcc aaagaagaag
       61 ataaagctga tgagcagtac gatacgggtc atgcgactgt taaatcggtg tcgttttcgc
      121 atactagttg cctgactcac aaaaggttcc ttgaagtatg tcccacgctg tggacggtac
      181 ttacattaag gcacaacagg acaaaatggt caattcttcg ttatgtaaaa aagcgtcagt
      241 ggatacatat tttaatgtta tggaagttaa tttaattatt tacaatgatg atgtaataat
      301 gatgaattaa catgataaac gtaataattc attaatcagg gttattttgt ttgatatata
      361 tcaattggaa ctttcatatg acacttagaa tcaatgctct ctatagtgat gaataatcat
      421 cattcgaagt caggtgggat gcctgtctga atacacctcc ttcaggatgt gggggattct
      481 gctg
//
LOCUS       DAJTNM010000351          479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTNM010000351 DAJTNM010000000
VERSION     DAJTNM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.358"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            15..173
                     /locus_tag="OO172_004331"
     CDS             15..173
                     /locus_tag="OO172_004331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514216.1"
                     /translation="MNDAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSN
                     LICRALRK"
     gene            345..>479
                     /locus_tag="OO172_004332"
     CDS             345..>479
                     /locus_tag="OO172_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5514217.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     L"
BASE COUNT          144 a          100 c          116 g          119 t
ORIGIN      
        1 ccaatacgga aaaattgaac gatgctgtgg aagagctgcg tcagttgctc gataaccacc
       61 atgacctgaa agtcgtggaa acaccaatct atggttatgt gtggctgaac atggaaacgg
      121 cacatcagct tgagttgcta tcgaatctga tttgccgggc cttgcgcaaa taattcctga
      181 acttcagaat catcttgctg ctgcttcgat tcagcaagga taaagggtat gatagtgaaa
      241 agggataaaa gcattgtcat ctgcggcagc tatgagtaat gttggcccta acgaatagcg
      301 gttgcttaaa cgaatccgac tctcacatta tcaggggtat aaaaatggaa actaccaagc
      361 cttcattcca ggacgtactg gaatttgttc gtctgttccg tcgtaagaac aaactgcaac
      421 gtgaaattca ggacgttgag aaaaagatcc gtgacaacca gaagcgcgtc ctgttgctg
//
LOCUS       DAJTNM010000352          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTNM010000352 DAJTNM010000000
VERSION     DAJTNM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.359"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..98)
                     /locus_tag="OO172_004333"
     CDS             complement(<1..98)
                     /locus_tag="OO172_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR5514218.1"
                     /translation="MYQMEKISTGIAYGTSGGSAGYWFLQWLDQVSP"
BASE COUNT          134 a          124 c           93 g          125 t
ORIGIN      
        1 ggactgacct gatccaacca ctgcaaaaac cagtagccgg cactgccgcc ggaggtgccg
       61 taggcaatgc ctgttgatat tttttccatc tgatacatat cccgcccccc cccgacagac
      121 ctgtgctatc ggaaagaaaa aaggccatca gcaaaactct gatgacctga atcaccttta
      181 ccaatattgt atgaaaaaac acgcacgact aattgacaat aattttcatt tccattaaaa
      241 aagatcgcgt agcattctta attcatgagg aactttaccc gccagcaatc tgagtagcga
      301 aagctgttca tccccaatgt ttttgctggc gggtcctttt ttctacggtc ctcctccccg
      361 gagggggggc atttcattat ttttctaatc gttactggtg agaaggcgga agaaaaagcc
      421 agttcttcgc ccctaaataa cagcatgatt ccagtccatg caaaatctga tttcta
//
LOCUS       DAJTNM010000353          474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTNM010000353 DAJTNM010000000
VERSION     DAJTNM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.360"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>474
                     /locus_tag="OO172_004334"
     CDS             <1..>474
                     /locus_tag="OO172_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514219.1"
                     /translation="LLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLA
                     AQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVAL
                     EVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTR"
BASE COUNT           90 a          162 c          127 g           95 t
ORIGIN      
        1 gccttctgac ccagatacat ccggcactgg agcgcgttct cggtccgaga cttgagcacc
       61 cggcggtact cgatcttctc cagcgatatc cctcaccaga aaaactcgct tcgctgggtg
      121 agaagaagct ggcagcccag ctctgcaaac ttgcgcctcg tctgggtaaa cgccttgcag
      181 cagacatagc tcaggcactg gccgaacaaa ccgtcgtcgt tcccggcacg aatgccgctg
      241 ccgtagtact gccacgtctg gcactccagc tcatcacgct gcgtaagcaa agagacgagg
      301 tggcgcttga ggtagaacag cgagttcttg ctcaccctct ttacccggtc ctgaccagta
      361 tgcccggagt cggtgtcagg accgcagcca gactcctcac cgaggtcgcc tgccgcgcct
      421 tcgcctctgc cgcacatctc gctgcttatg ctggccttgc gccggtaact cggc
//
LOCUS       DAJTNM010000354          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTNM010000354 DAJTNM010000000
VERSION     DAJTNM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.361"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            157..>472
                     /locus_tag="OO172_004335"
     CDS             157..>472
                     /locus_tag="OO172_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5514220.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTY"
BASE COUNT          173 a           77 c           88 g          134 t
ORIGIN      
        1 gggtctggct ggctaacacc ccattataag gggtacgaat aaagtaacgg gggaagcaat
       61 acctggagaa agagtacaaa gcaaactgta ttaaacttaa taagttatat tgtatttcac
      121 tagagttctt atcgttgata agaaattctg gttataatga ttaaatcaac aaatatacag
      181 gtaatcggtt ctggtattat gcatcaaata aacaacatac actcgttaac tctattttct
      241 ttgcctgtga gtttgagtcc atcttgtaat gaatattatt taaaagtctg gagtgaatgg
      301 gaaaagaacg gtaccccagg cgagcaacgc aatatcgcct tcaataggct aaaaatatgt
      361 ttgcaaaatc aaggggcgga attaaatcta tctgagttag atttaaaaac attacctgat
      421 ttaccgcctc agataacaac actggaaata agaaaaaacc tattaacata tc
//
LOCUS       DAJTNM010000355          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTNM010000355 DAJTNM010000000
VERSION     DAJTNM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.362"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>464
                     /locus_tag="OO172_004336"
     CDS             <1..>464
                     /locus_tag="OO172_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5514221.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DAJTNM010000356          462 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTNM010000356 DAJTNM010000000
VERSION     DAJTNM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.363"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..92)
                     /locus_tag="OO172_004337"
     CDS             complement(<1..92)
                     /locus_tag="OO172_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HCR5514222.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            148..300
                     /locus_tag="OO172_004338"
                     /pseudo
     CDS             148..300
                     /locus_tag="OO172_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          135 a           90 c           93 g          144 t
ORIGIN      
        1 tcaactctag gataaatatt tctcactata tattcagtat ttgcagtatt tattggaaat
       61 acgttcttaa tattcttgat gggactaatc attttaatct ctatactcaa atataaacat
      121 taccatgaac aatataattg ttcctaagtg gaatgtctcc acggagaaca ctttatctat
      181 cgggaaatag tgcgggcaac agtgtttaat tatatcgagt gcgattacaa tcgctggcga
      241 cggcaccgtt ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa cctcgcttag
      301 gcctgtgtcc atattacgtg ggtaggctca cagagcctca cttacgactg cgtccatatt
      361 atgtgggtag gatcaataaa gacgttttat atgtgtctgc ttgcagccag aaaatttata
      421 ccctgcaggt atcataaaca tgcttgataa cttaattggg gc
//
LOCUS       DAJTNM010000357          459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTNM010000357 DAJTNM010000000
VERSION     DAJTNM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.364"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
BASE COUNT          162 a           97 c          100 g          100 t
ORIGIN      
        1 aaataacgga tggcgtagtt ttacactgag aaatgaaagg gatttgaaaa gaaaaaaacg
       61 caaaagcggg cgaaacgata tatacagtaa ggaaagcact ctatccaaca aaccacccac
      121 agttaatcgg aataaaagca gagtgcttat gaatgatcgc acgcccgaag gttagtattt
      181 ctgcacagca attttgcaaa aaaaggcgat cattcataac ttaaacgtct ttcagtcact
      241 ccgggatttc ccatcatcgc agactgaaag actctgactg gagcgggcag cgggagccta
      301 cgacaaatcg aagacaacca gctaacctat tgtttcttaa agacaggtga atccagttgg
      361 ttgtagtgat agtcacacat ttagagatag aaggtcaaga cttgaagcac gtaatattcc
      421 ctctaaagga atagaactca cggtctgact acccggccc
//
LOCUS       DAJTNM010000358          448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTNM010000358 DAJTNM010000000
VERSION     DAJTNM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.366"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(139..>448)
                     /gene="ydcF"
                     /locus_tag="OO172_004339"
     CDS             complement(139..>448)
                     /gene="ydcF"
                     /locus_tag="OO172_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF218 domain-containing protein"
                     /protein_id="HCR5514223.1"
                     /translation="RMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERY
                     LSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR
                     "
BASE COUNT          135 a          102 c          111 g          100 t
ORIGIN      
        1 atcatcagag agacggcatc gggaaaggac aattctgtgg agggcttcat aaaaacaagt
       61 gtaaaaggaa taaaaggcag aaataaaagt caggagtcat gcccgtttgc aaacaagtgg
      121 ttcacaaacg ggcaattttt aacgtaacga gcgactttcc atcgcctcga tgagcaccgc
      181 atcatgtttc agcgtttgcc atgcatggat gacttctgcc ggaaaatcaa cgtgaacgat
      241 aaaatctcgc ccgcggggac cgtagccatc gctatcatcg tgtaaacgcg gcagctcgcc
      301 agtgagtagt gagagataac gctcaaccgg ccataatcct tgtaactgat taataaagat
      361 tacactgtct gcgttatttc ctaactgagg aacgaatccg ggataactta accagcgtgg
      421 tgcatcggga ttgtccccgg tcatacgg
//
LOCUS       DAJTNM010000359          443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTNM010000359 DAJTNM010000000
VERSION     DAJTNM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.367"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..237
                     /locus_tag="OO172_004340"
     CDS             <1..237
                     /locus_tag="OO172_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514224.1"
                     /translation="SIEQDINQPVRQEIDAFFCAFREKVEQLRNTLIQSSEDHKRDQQ
                     AQERLTRRLQALNERVPELITDSKALREELETML"
     gene            234..>443
                     /locus_tag="OO172_004341"
     CDS             234..>443
                     /locus_tag="OO172_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR5514225.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINAS"
BASE COUNT          122 a          105 c          117 g           99 t
ORIGIN      
        1 tccattgagc aggatattaa ccagcccgtc cgccaggaaa tcgatgcctt tttctgcgca
       61 ttcagggaga aagttgaaca actgcgtaac acgctgattc agagctctga agatcataaa
      121 cgcgatcagc aggcacagga acggcttacc aggcgacttc aggcattaaa tgaaagggtt
      181 cctgagctca ttactgacag taaggcgctg agggaagagc tggagacaat gctgtgactt
      241 caccatttat tcaacaaatt gctgataacc gggtatgtca ggtactcacc tgtcttcctg
      301 aaaaatttgt ggttgatttc gcaaacggaa tcgatgttgc acaggagcat atccgcacgg
      361 ccggagagcg cacgttcttc cggcgtttaa aagagggact gaccggcgaa ggcgctgcac
      421 ggcagaatgc catcaatgct tcg
//
LOCUS       DAJTNM010000360          433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTNM010000360 DAJTNM010000000
VERSION     DAJTNM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.368"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            51..433
                     /locus_tag="OO172_004342"
                     /pseudo
     CDS             51..433
                     /locus_tag="OO172_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
BASE COUNT           89 a          105 c          109 g          130 t
ORIGIN      
        1 ctatcactta aataagtgat actggttgtc tggagattca gggggccagt ctactgcccc
       61 tgatgatgtg gggagcaatg cgctatggtt ataagctgat ttcgctgctc tgggcggtcg
      121 tgttgatgat cagcatccac agctatcaaa attacattcc catttatcct ggctatacca
      181 cgcagctgac cataacctcc tccagttatc tggtattctc ttttattgtc aattatatgg
      241 ctgtactggc aactcgtcag cgagcggtag tcagacgcat tcagcggctt gcgtatgtgg
      301 acccggtggt tcatctgcca aatgttcgcg ccctgaatcg cgcgttgcgt gatgccctct
      361 ggtctgcgct ttgttattta cgcatccctg gcatggaaat gctggttaag aactatggca
      421 tcatgctgcg gat
//
LOCUS       DAJTNM010000361          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTNM010000361 DAJTNM010000000
VERSION     DAJTNM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.370"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..386
                     /locus_tag="OO172_004343"
     CDS             <1..386
                     /locus_tag="OO172_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514226.1"
                     /translation="ITQVHIKDALIVKEQGGLGHKACISGQGDMPFKALLTHLICLGD
                     DEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIPYRQMSETPLPENHLLDARLRKEKED
                     AINQINHVRNVLQQIKQEANHLLNH"
BASE COUNT          136 a           86 c           92 g          104 t
ORIGIN      
        1 atattaccca ggtccatatt aaagatgcct tgatcgttaa agaacagggt ggcctgggtc
       61 ataaagcctg tatttcaggt cagggtgata tgccattcaa agcgttatta acgcacctta
      121 tctgccttgg tgatgatgag ccgcaggtga cggcatatgg tctggaagaa gaggttgatt
      181 actatgcgcc ggcgttccgc tttgaagacg aagatgataa tccgtggatc ccttatcgcc
      241 agatgagtga aacaccccta ccagaaaatc atttactgga tgcgcggtta cgtaaagaaa
      301 aagaagatgc aattaatcag ataaatcatg tgcgtaacgt actacaacaa atcaaacaag
      361 aggcaaacca tcttctgaac cactaataag ttttaactca tacgcagtta ataaaacc
//
LOCUS       DAJTNM010000362          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTNM010000362 DAJTNM010000000
VERSION     DAJTNM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.371"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
BASE COUNT          123 a           81 c           88 g          126 t
ORIGIN      
        1 cggttaactg tccagtcgac gggcttttgt gtgatatcag tcacataaat atagggggta
       61 gagaggtaat tgacgtgacg ctcatcacaa aacgcctgtg taaaaaagaa acaaggcggt
      121 gattttcgct aaaaatggca tttaaatgtg agtaatgtca catttttgtt ttgatggttt
      181 gttgaatctt tgtgatctga atcacaagat attttcttac ggcgttcacc tgatgtgatc
      241 tacagcatgt tatgccttcg tcatacaatc gttatgtaac taagcaactc atcacctcta
      301 aaaattattc tttggtgtta tggcgagaat cgcgaacccg gattattgtc aaaatatcag
      361 cctgactccc ttctggctgc cagccaaaaa acaacactga taaacgtgaa tccgaggc
//
LOCUS       DAJTNM010000363          417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTNM010000363 DAJTNM010000000
VERSION     DAJTNM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.372"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            64..>417
                     /locus_tag="OO172_004344"
     CDS             64..>417
                     /locus_tag="OO172_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5514227.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKT"
BASE COUNT          114 a           94 c           98 g          111 t
ORIGIN      
        1 tttactttcc tcttttgatg atcttaaatg tcttattttt cgtaatgtgt ataacaagga
       61 atagtgatga aatttaaaaa atgtcttctg cctgtggcaa tgttagcgtc attcactctg
      121 gcaggatgcc agtcaaatgc tgacgatcat gccgccgatg tttatcaaac cgatcaactg
      181 aataccaaac aagaaactaa aaccgttaat attatttcca ttcttcccgc aaaagttgcc
      241 gtagacaact cccaaaataa acggaacgca caagccttcg gcgcgcttat tggcgcagtc
      301 gctggcggtg ttatcggcca caacgtcggt tctggcagca attccggaac gacggcaggg
      361 gcagttggcg gcggagctgt aggcgcggca gcgggttcta tggtgaatga taaaacc
//
LOCUS       DAJTNM010000364          413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTNM010000364 DAJTNM010000000
VERSION     DAJTNM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.373"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            134..>413
                     /locus_tag="OO172_004345"
     CDS             134..>413
                     /locus_tag="OO172_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514228.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERI"
BASE COUNT          107 a          115 c           95 g           96 t
ORIGIN      
        1 cgcttaaccg ttagtagctc acctactcaa tacctaattt aaacaccttt tgagatgacc
       61 gatgcgggga gacccgtagg ccttactgtg cctgcgatac cgatagtctc aactctcaag
      121 aggagaatta cctatgatgt tcaacaacaa caactggaag ctgtcagtca ccgacatcaa
      181 cctttatgaa aacactgtga gtctggacgg tcagtcctat ccgttgtcgc tcgctatcaa
      241 gaccctcatt ccgggctacc tgtcaggctt accgtccacc agccgcgagg cgatggagtt
      301 actggaagct ctggctgaag ctggcgtgac cattggtaac ttcttcagta atgacctgat
      361 gaccgcctac caacgccgcc agatgaacaa acgagctgaa gctgagcgta tcg
//
LOCUS       DAJTNM010000365          413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTNM010000365 DAJTNM010000000
VERSION     DAJTNM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.374"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>413
                     /locus_tag="OO172_004346"
     CDS             <1..>413
                     /locus_tag="OO172_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514229.1"
                     /translation="WRRFTDRFVNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKTGIKM
                     KAETETGYEPSILIQMEKQMNIETGQVWRTARILKDRSTRIDGQVFSNPTFKNFLPHI
                     EFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEKR"
BASE COUNT          131 a           91 c           97 g           94 t
ORIGIN      
        1 gtggcgacgc tttaccgatc gatttgttaa cagtcaggcg cacattatta tgtgcgggcg
       61 tgcaggatat gagtatgact ttttcgaagg tgatgatggc aaacgccagt tggcaaaaac
      121 cggtataaaa atgaaagcag aaacagaaac cggttatgaa ccctccatcc tgatccagat
      181 ggagaagcaa atgaacatag aaaccggtca ggtgtggcgc accgccagaa tactaaaaga
      241 tcgctccacc cgtattgatg gacaggtatt ttcaaaccca acatttaaaa actttcttcc
      301 tcatatcgag tttcttaacc tgggaggtga acacctcggc atcgatacct cacgggataa
      361 cagtgaatta tttgccaacg acggtacacc gacatggcta aaagaaaagc gtg
//
LOCUS       DAJTNM010000366          413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTNM010000366 DAJTNM010000000
VERSION     DAJTNM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.375"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(246..>413)
                     /locus_tag="OO172_004347"
     CDS             complement(246..>413)
                     /locus_tag="OO172_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5514230.1"
                     /translation="LSYTVGSLLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRN
                     AGHTPKPVAHV"
BASE COUNT          150 a           78 c           92 g           93 t
ORIGIN      
        1 tgatggggcg cttacttcct gaacattccc agccatagac aaatccttcg ccaaaattaa
       61 aggggcatct gtaaaacgag ggattatgcg cggcaaataa gaagtgcgga aatagcgaaa
      121 tgcaaaatta taacctgctg cacattttat ttttaaacga ataaaatttg gaaaaaagca
      181 agtcagatag aagaaacaaa aaagcagagt gtgaaacagt aagttcacac tttgcttgaa
      241 ttcatttaca cgtgagcaac gggtttcggc gtatgaccgg cgttgcgaag cagcatcagc
      301 aaatagataa acgatacgct ggcgatcatg ataaacaata aattatcgga gaaattctgc
      361 attagcatag cggtaaatga cgggccaagc agacttccca cggtatagct caa
//
LOCUS       DAJTNM010000367          410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTNM010000367 DAJTNM010000000
VERSION     DAJTNM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.376"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..352)
                     /locus_tag="OO172_004348"
     CDS             complement(<1..352)
                     /locus_tag="OO172_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5514231.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILM"
BASE COUNT          143 a           95 c           78 g           94 t
ORIGIN      
        1 gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc attaatgacc
       61 agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc aaaataccgg
      121 aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca ccaaactctg
      181 tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat gacgaacggt
      241 cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc tgaatacgac
      301 gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc atttttagct
      361 ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
//
LOCUS       DAJTNM010000368          405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTNM010000368 DAJTNM010000000
VERSION     DAJTNM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.377"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>405
                     /locus_tag="OO172_004349"
     CDS             <1..>405
                     /locus_tag="OO172_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5514232.1"
                     /translation="YIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIRNTTGFCL
                     PQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRC
                     YNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
BASE COUNT          130 a           81 c           66 g          128 t
ORIGIN      
        1 tatattgtga attatccaga agcaggtctt tatcttgcag aaatgcttta ttatcccatt
       61 gatctaattg aaaagtttca aaaattttat gcgataactg atcaaattcg taacacgaca
      121 ggtttctgct tacctcagaa ccccgagtta atatattgtt gggagcaact aaaaacatct
      181 atttcccgag gcttctcaac gcaaattcag gaacacttag caatgggcgt tctactttca
      241 ttaggagcac atcatgttaa ttgtttactt ttatcagata gtaaacaatc attaataagt
      301 cgttgttata acctaatgct atccgaacct agaacaaaat ggacagcaaa caaggtagcg
      361 agatatctct acatttctgt ttccacattg catcgccgtc tggca
//
LOCUS       DAJTNM010000369          401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTNM010000369 DAJTNM010000000
VERSION     DAJTNM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.378"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..>401)
                     /locus_tag="OO172_004350"
     CDS             complement(<1..>401)
                     /locus_tag="OO172_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514233.1"
                     /translation="IIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKI
                     REGDAKEIISIKNSIVEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAV
                     GIIKLCGLEAVEAVFGVDERNKIVRKLCQRIA"
BASE COUNT          113 a          103 c           71 g          114 t
ORIGIN      
        1 gcaattcgct gacacaattt cctgacgatt ttattgcgtt catcaacacc aaacaccgct
       61 tccacggctt ccagaccaca cagtttgatg atccccacag ccagatactg attattgttg
      121 gactcaataa tcgccatatt tttaataaaa ccgctttcct gatcgtaaga gatcagtgag
      181 agcaatttat ttgaccgttc aatttcggca tctttcattt ccacgatcga atttttgatg
      241 ctgataatct ctttcgcatc gccttcgcga attttcagag cctcgatctg atccgttgag
      301 tcgggcaaaa acgccagcgt attaaccagc tcggtcattt cagaaaattg ccgacggaac
      361 acacgagtgg tcatgaaata taccaccagc aacacgataa t
//
LOCUS       DAJTNM010000370          400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTNM010000370 DAJTNM010000000
VERSION     DAJTNM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.379"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>400
                     /locus_tag="OO172_004351"
     CDS             <1..>400
                     /locus_tag="OO172_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR5514234.1"
                     /translation="AHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQ
                     IFSWWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQM
                     AQESGGSATVRLNTLDWLGGQGREQADNEW"
BASE COUNT           95 a           94 c          121 g           90 t
ORIGIN      
        1 tggcgcatta ttccgcagat acacgggagc agttactcat cctggccgat caggttcacc
       61 ataaactgaa tcatcttgaa gaaaaactcc accgtgttga ccaggttcag cgggcgcagt
      121 tacatcttga gcagatattc tcatggtgga gcgccgggcg atacgcgtca ttttcccctg
      181 ccggacgttg ttatgtggca cttgaagagc ttcgctgggg cgcgtttggt gatgtgatac
      241 gtcagggcga aacaggccag gttaaccagc tactggatat tctcagacat aaagcattaa
      301 cgcagatggc acaggagagt ggcggtagtg caaccgtgcg tctgaacact ctggactggc
      361 ttggtggtca gggccgggag caggcagata acgagtggca
//
LOCUS       DAJTNM010000371          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTNM010000371 DAJTNM010000000
VERSION     DAJTNM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.381"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..215)
                     /locus_tag="OO172_004352"
     CDS             complement(<1..215)
                     /locus_tag="OO172_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514235.1"
                     /translation="MKAVARANNHPYKSVFADFITGHPSCTVCFWETFHKMYPDSPYE
                     YVTFCHTCRRFDLYETEAEMKADDPKW"
BASE COUNT           85 a           97 c          107 g          109 t
ORIGIN      
        1 caccacttcg ggtcatccgc cttcatctcc gcttctgttt cgtataaatc aaaacgacgg
       61 caggtatggc agaacgtgac gtattcgtac ggtgaatccg ggtacatttt gtggaaggtt
      121 tcccagaaac agaccgtgca ggagggatga ccggtgataa agtctgcaaa tacggactta
      181 taagggtgat tattggctct ggcgacggct ttcagaaccc tctttcacca ttctggtgtg
      241 gactttctgg tgctccaggt tgtgtgacat gggaactcat tctggatggt tactctgaaa
      301 gctcatattc tgccacaccc cgatttgcag ccgccaggct gccgtggttc ttacgccaga
      361 agggatttgt aactggtctg gtcggctccg gcgtatca
//
LOCUS       DAJTNM010000372          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTNM010000372 DAJTNM010000000
VERSION     DAJTNM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.382"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>397
                     /locus_tag="OO172_004353"
     CDS             <1..>397
                     /locus_tag="OO172_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5514236.1"
                     /translation="RIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLK
                     AAIKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDL
                     FNNEVISYSLSERPVMNMVENMLDQAFKKL"
BASE COUNT          120 a           85 c           97 g           95 t
ORIGIN      
        1 cgtattggtg agatttatca cgagaataga ggccgatacg gataccgtag ggtaacgctg
       61 tctcttcatc gagaagggaa acagattaac cataaagctg ttcagcgcct gatgggaacc
      121 ctctcactta aagcagcgat taaggtcaag cgataccgct cttacagagg agaggtaggg
      181 caaaccgccc ctaatgttct ccaaagagat ttcaaggcta cgcggccaaa cgagaagtgg
      241 gttaccgatg ttactgaatt tgcagtcaat gggcgcaagc tgtatttgtc tccagtaata
      301 gatctcttca acaacgaagt tatttcttac agcctttcgg aaagaccagt gatgaacatg
      361 gtcgagaata tgctcgatca ggcattcaaa aagctta
//
LOCUS       DAJTNM010000373          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTNM010000373 DAJTNM010000000
VERSION     DAJTNM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.383"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(224..>394)
                     /locus_tag="OO172_004354"
     CDS             complement(224..>394)
                     /locus_tag="OO172_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5514237.1"
                     /translation="RGMTKRKMAELLNDAHPEWCFRTCCSRVDVWLNLAEYMLYLPMR
                     DAFSSGDLKTVC"
BASE COUNT          106 a          110 c           98 g           80 t
ORIGIN      
        1 tgaactggtg gcgaactgtg cagggttggc gtaaccgggt acggaaaccg gcgagccttt
       61 cggctccccc acacagcccg ccataaatcg cgaatgtgac tgtgcaaacg atatgaaaaa
      121 agacgcgggc gcgtctcata tcgctccgta aacatccggg acgccaatcc cgacgccaga
      181 ttttgctgcg cgtgaggaat atagccccgg ataacagatt gagtcaacaa acggttttta
      241 gatccccgga agagaatgca tcacgcatcg gtagatagag catatattca gcaagattta
      301 gccatacatc tacccgactg cagcacgtcc ggaaacacca ctcagggtgt gcgtcattca
      361 gcaattcagc cattttacgc ttagtcatcc cccg
//
LOCUS       DAJTNM010000374          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTNM010000374 DAJTNM010000000
VERSION     DAJTNM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.384"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..>391)
                     /locus_tag="OO172_004355"
     CDS             complement(<1..>391)
                     /locus_tag="OO172_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5514238.1"
                     /translation="IPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIFTNR
                     FLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGIYMI
                     NNSRTANGTDGQAYMDNNGNWLCGSQVI"
BASE COUNT           98 a          104 c           79 g          110 t
ORIGIN      
        1 aataacttgc gaaccgcaaa gccagttccc gttattatcc atgtacgcct gaccatcggt
       61 gccattggct gtccttgagt tattaatcat gtagatgcca aattgcctat tccccagccc
      121 accaattacg aatttacggt cggtatggtc ctggcgaagc aatgcctgcg caccatcgtt
      181 ggataccgca ttacggccca taataacgtt ctggtcacgc atatgaatcc acatgcctgt
      241 actgctgtta attaaaaaac ggtttgtaaa tatatctcct gtaacaacca gaccatgccc
      301 catgcttatc cggccagttc tgagattaag cgtaaagggg cgtagtggcc ctatattacc
      361 attttcccct tcattctctc gtgtagggat g
//
LOCUS       DAJTNM010000375          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTNM010000375 DAJTNM010000000
VERSION     DAJTNM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.385"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(1..313)
                     /locus_tag="OO172_004356"
                     /pseudo
     CDS             complement(1..313)
                     /locus_tag="OO172_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
BASE COUNT          105 a          105 c           91 g           90 t
ORIGIN      
        1 gtatccgatt tgccgttgta aaacggtgcc tgattaaacg gacagggatc atccattacc
       61 ggggtgtcca tcctggactc gctgaagccc aggtaattaa aatcgtcctt taccgtcagc
      121 cggcgactga cacccacaat ctttccccag acatcaagac catacttttc tgcggtatcg
      181 atgttccaga taagaccata aaaatcactg ataaaactgt cggggaaagc gctgcgttaa
      241 agctgttaat gagggcattg agtcgggggc tggcagcata ctgcgcaagc acagtggcag
      301 ccacattctg cacgttatgc ctcctggagt ttcacactga tatccgacac atccagaacc
      361 ggaatctcat ctatcccgaa agtgacagcc g
//
LOCUS       DAJTNM010000376          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTNM010000376 DAJTNM010000000
VERSION     DAJTNM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.386"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..147
                     /locus_tag="OO172_004357"
                     /pseudo
     CDS             1..147
                     /locus_tag="OO172_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
     gene            144..>387
                     /locus_tag="OO172_004358"
     CDS             144..>387
                     /locus_tag="OO172_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR5514239.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIATSPDLSEAAVRLEALWDHLGNNQVLSVNR"
BASE COUNT           87 a           92 c          124 g           84 t
ORIGIN      
        1 gactagtgca gtgaagagca gcatccggtg atctggtcaa ccacgcaggc tgcagaacac
       61 ttaccggttc gcatgcctcg cctctgctct gcagaacgcc tctctgaaag tatggtcgat
      121 gaaatatttc agaagggggc tgcatgagta cagaaatgaa aacggggctg gtgctgtccg
      181 gtggcggggc ggtgggcgct tatcaggcgg gagtggttaa ggcactggca gagtgtgata
      241 cacagatcag catggtttca ggggccagca ttggcgcatt caatggtgcc attatcgcga
      301 cctctcccga tctgtcagaa gctgccgtac gcctggaagc gctctgggat catctgggga
      361 ataatcaggt gctgtcggta aaccgat
//
LOCUS       DAJTNM010000377          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTNM010000377 DAJTNM010000000
VERSION     DAJTNM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.387"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..369
                     /gene="rrrD"
                     /locus_tag="OO172_004359"
                     /pseudo
     CDS             1..369
                     /gene="rrrD"
                     /locus_tag="OO172_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
BASE COUNT          110 a           92 c          102 g           81 t
ORIGIN      
        1 gatatcgttg gcgtatggac tgtatgccac ggacacaccg gaaaagacat catgctcggt
       61 aaaacgtata ccgaagcaga atgcaaagcc ctcctgaata aagaccttgc cactgtcgcc
      121 agacaaatta acccgtacat caaagtcgat ataccggaaa caacgcgcgg cgctctttac
      181 tcgttcgttt acaacgtggg cgctggcaat ttcagaacat cgatgcttct tcgcaaaata
      241 aactagggcg atatcaaagg cgcatgtgac cagctacgtc gctggacata tgctggcggt
      301 aagcaatgga aagggctgat gacccgtcgt gagattgagc gtgaagtctg tttgtggggg
      361 cagcaatgag cagagtcacc gcgat
//
LOCUS       DAJTNM010000378          384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTNM010000378 DAJTNM010000000
VERSION     DAJTNM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.388"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            238..>384
                     /locus_tag="OO172_004360"
     CDS             238..>384
                     /locus_tag="OO172_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514240.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSIS"
BASE COUNT          114 a           82 c           77 g          111 t
ORIGIN      
        1 ttaaagaaaa cgcctgtact aaaaccgacc cgtggtacag gcgaagaata cgggtctaca
       61 tcggaagcgc ctatcatatt tatttgtatg acaaataaaa ccataatcct tgcccatacg
      121 tccatctggc ttatttttaa tcaattcacc cgatctttga tctcatcaac ggtatcaaaa
      181 taaaccagcg tataacgttc aaatcgcaca taagattcga taaaccatgg aggttatatg
      241 aaaaaactgg cacttatttt gtttatggga acgcttgttt ccttttatgc cgatgccggg
      301 cgcaaaccct gttctggttc gaaagggggg atctcacact gtacggcagg cggcaaattt
      361 gtctgtaatg atggttctat tagt
//
LOCUS       DAJTNM010000379          382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTNM010000379 DAJTNM010000000
VERSION     DAJTNM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.389"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            1..331
                     /locus_tag="OO172_004361"
                     /pseudo
     CDS             1..331
                     /locus_tag="OO172_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           95 a          111 c          102 g           74 t
ORIGIN      
        1 gtaaggtgga acggatggtg cagtacaccc gtaacagttt ttacatccca ctaatgactc
       61 gcctgcgccc gatggggagc actgtcgatg ttgaaacagc cagccgccac ggtctgcgct
      121 ggctgcacga tgtcgctaac caacgaaagc atgaaacaat ccaggcccgt ccctgtgatc
      181 gctggctcga agagcagcag tccatgctgg cactgcctcc ggagaaaaaa gagtatgacg
      241 tgcatcctgg tgaaaatctg gtgagcttcg acaacccccc cagcatcatc cactctccat
      301 ctacgactca ttctgcagag gagtggcgtg atgatggaac tgcaacatca acgactgatg
      361 gcgctcgccg ggcagttgca ac
//
LOCUS       DAJTNM010000380          379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTNM010000380 DAJTNM010000000
VERSION     DAJTNM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.390"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
BASE COUNT          104 a           84 c           98 g           93 t
ORIGIN      
        1 tttaatgtgt gtcatacact cagtggcgta atcgttgaca atatggtgca tgtaatcgta
       61 agcgtccagc gaaatccatc tggtcataat ctgaagcatc cgacaaagtg gtgtccacca
      121 aataagtgat ctgcgcccaa tatcctgagc cggaactttg acaaaggcca acgaaaaatg
      181 ggagcggatg ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg ccagttctcc
      241 gatctgttta accgggaaat tatctcctac agcttgtcag aaaggtcggt gatggagcat
      301 cgttaatacg atgcattcac agtgcccggc cagaggatgc cccgtcgctg catatggatc
      361 agggttggca atatcgaat
//
LOCUS       DAJTNM010000381          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTNM010000381 DAJTNM010000000
VERSION     DAJTNM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.391"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>378
                     /gene="fimC"
                     /locus_tag="OO172_004362"
     CDS             <1..>378
                     /gene="fimC"
                     /locus_tag="OO172_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5514241.1"
                     /translation="STYLIQSWEENADGVKDGRFIVTPPLFAMKGKKENTLRILDATN
                     NQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPPDQAAEK
                     LRFRRSANSLTLINPTPYYLTVT"
BASE COUNT          119 a           82 c           81 g           96 t
ORIGIN      
        1 atagtaccta tttaattcaa tcatgggagg aaaatgccga tggtgtaaag gatggtcgtt
       61 ttatcgtgac gcctcctctg tttgcgatga agggaaaaaa agagaatacc ttacgtattc
      121 ttgatgcaac aaataaccaa ttgccacagg accgggaaag tttattctgg atgaacgtta
      181 aagcgattcc gtcaatggat aaatcaaaat tgactgagaa tacgctacag ctcgcaatta
      241 tcagccgcat taaactgtac tatcgcccgg ctaaattagc gttgccaccc gatcaggccg
      301 cagaaaaatt aagatttcgt cgtagcgcga attctctgac gctgattaac ccgacaccct
      361 attacctgac ggtaacag
//
LOCUS       DAJTNM010000382          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTNM010000382 DAJTNM010000000
VERSION     DAJTNM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.392"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..>378)
                     /locus_tag="OO172_004363"
     CDS             complement(<1..>378)
                     /locus_tag="OO172_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5514242.1"
                     /translation="LALVVCVVWQVFSRYVLNQPSTLTDELARFLMIWVGLLGAAYTV
                     GAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVIVTGGLKLIDKTLATSQVSA
                     AMQIPMGYVYIILPLSGVVMMFYA"
BASE COUNT          123 a           86 c           77 g           92 t
ORIGIN      
        1 gcataaaaca tcattaccac tccacttaat ggcagaatga tatatacgta tcccatcggt
       61 atttgcatag cggcagatac ctgtgatgtt gccagtgttt tatcaattaa tttcaggcca
      121 ccagtcacaa tgaccgaacc ggcaaaacct aaaatgagaa cgttaatgac tatactaaga
      181 agtaattgtt tgcgcttatt cagggcgaga gcaaataaat caatagagag atgtcgctgc
      241 gcgccgacgg tatatgcagc acctaataat cctacccaga tcatcaaaaa gcgtgccagc
      301 tcatcggtta atgtacttgg ctggttaagt acgtagcggc taaatacttg ccagactacg
      361 catacaacca gagcaagc
//
LOCUS       DAJTNM010000383          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTNM010000383 DAJTNM010000000
VERSION     DAJTNM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.393"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
BASE COUNT          104 a           69 c           73 g          128 t
ORIGIN      
        1 attttcgctt gatgtatctg cataacaaaa acaatatgcg ccatttttgc attactaata
       61 agaaaaagca aaaaacatcg atattcattt ttggtcaatg gattttctgg cctatcgtta
      121 ttattgtgct actggttatc attttcatct tgccaggatc gcacgtaatc acgttattcg
      181 gggactttat ctgcgggata ttacccttca ggtaagtcct tttctcgcca gcatattgga
      241 gcgagatcaa tattgatcag ctgtgaagag ggctgatgtg tagtggaaca aaaactgacg
      301 ttatatcact gctaatgaga tatcagtcat ctgaacactt atgtctttaa ttattctcgt
      361 ggttcactgt aggc
//
LOCUS       DAJTNM010000384          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTNM010000384 DAJTNM010000000
VERSION     DAJTNM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.394"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..>373)
                     /locus_tag="OO172_004364"
     CDS             complement(<1..>373)
                     /locus_tag="OO172_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5514243.1"
                     /translation="SAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPYASLA
                     ILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGSAALG
                     VGKDLGALGAISFDVTHARAN"
BASE COUNT          101 a           98 c           81 g           93 t
ORIGIN      
        1 aaattcgcac gagcatgtgt cacatcgaaa gaaattgcgc ccaaagcgcc aagatcttta
       61 ccgacaccca gagctgcaga accataattt tcagcagcct gcatacctcc atataaagtg
      121 atcccgtggg aaaaaccatg aagtatttgc gcctgaagga cgtccggtgt aaatccgtct
      181 tcatcgcgca attcacccac gctgacatca acatctgtca gaccttcacg tttaagaatc
      241 gccaatgaag cataaggttg ggtgaatcgt tgttcgctac cgtcttcttc tttgatggtt
      301 acgtccaaat cgccacctgt acttgcggtg ttgagatcgg tgatctcaaa agcaccttgt
      361 ggaacggctg act
//
LOCUS       DAJTNM010000385          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTNM010000385 DAJTNM010000000
VERSION     DAJTNM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.395"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            65..>372
                     /locus_tag="OO172_004365"
     CDS             65..>372
                     /locus_tag="OO172_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514244.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     V"
BASE COUNT           94 a           83 c           94 g          101 t
ORIGIN      
        1 cgcaggcgct gcgcttatgt actgcaccca ttttgttgga cgatgaaatg gaatagcccc
       61 tgatatgtca aagccaaaat acccttttga aaagcgcctt gaagtcgtga atcactactt
      121 cactactgat gatggttaca ggatcatctc ggcacgtttt ggtgtccccc gaacccaggt
      181 caggacatgg gttgccctct atgaaaaaca tggagaaaaa ggcttaattc ccaaacctaa
      241 aggtgttagt gctgatccag agttgcgtat taaggtcgtg aaagctgtga ttgagcagca
      301 catgtccctt aatcaggctg ctgctcactt tatgcttgct ggtagtggtt ctgtagccag
      361 gtggctgaag gt
//
LOCUS       DAJTNM010000386          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTNM010000386 DAJTNM010000000
VERSION     DAJTNM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.396"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            68..365
                     /locus_tag="OO172_004366"
                     /pseudo
     CDS             68..365
                     /locus_tag="OO172_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          109 a           75 c           93 g           88 t
ORIGIN      
        1 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca
       61 ttacccgcaa aacgataatt ccggtagctc acatcaactc ggactcagcg gtacggcgct
      121 ggaaagaaat aacctttcat ggaatttaat gcagtcctat aacagtcatg atgatgaggt
      181 tggcggtaat atgtccctga cctatgatgg ctcttatggc acggtgaacg gcagctataa
      241 ctacagccaa aattcccaga ggctgaatta tggtatcaga gggggaattc tggcacacag
      301 cgaaggggta acgttaagtc aggagttagg tgaaactatt gctcttgtta aagcacctgg
      361 ggccg
//
LOCUS       DAJTNM010000387          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTNM010000387 DAJTNM010000000
VERSION     DAJTNM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.397"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..>364)
                     /locus_tag="OO172_004367"
     CDS             complement(<1..>364)
                     /locus_tag="OO172_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5514245.1"
                     /translation="RVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKKAK
                     EYLDKGVHIYGGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCMDS
                     LFATTNKLGEVANLRVTF"
BASE COUNT           98 a           98 c           69 g           99 t
ORIGIN      
        1 ctgaatgtca cgcgaagatt ggccacttcg cctaatttat tggtggtggc gaagaggcta
       61 tccatgcagg aaaaaccttt atctaaaaaa ttatcccgca tcccagaacg ggtaaccgtg
      121 atcacgggta acgtatcctc aaccgttttt ctgtcaatca tgtcttttcg acaagtagcc
      181 agaaagtctt ccgggccacc ataaatgtgg acacctttat caaggtattc cttcgctttc
      241 ttacaatcta cctcaacata ctgaccattg gcgtacatca tgcccaacct ggaccatgct
      301 aaattcatac cttgctgcgc ggcctgatta aatagtgcat acgctttggc ataatccacc
      361 cgca
//
LOCUS       DAJTNM010000388          363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTNM010000388 DAJTNM010000000
VERSION     DAJTNM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.399"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..282)
                     /locus_tag="OO172_004368"
     CDS             complement(<1..282)
                     /locus_tag="OO172_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5514246.1"
                     /translation="MYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAILAGVATTAV
                     WLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN"
BASE COUNT           78 a          106 c           87 g           92 t
ORIGIN      
        1 attatcccgt gccgtccctt tattctgaag gtctgacaga ttgttcttct gccgcaggaa
       61 tatccttttc ggatcctcaa gaagatttac ccagcccgca ctgtcggagc cttcaggatc
      121 ggtcaggtta tcgtccgtgg tattcagcca gaccgctgtt gttgcgactc cggcgagaat
      181 ggctcctttt gcatatccac caatggcccc cgcaaaaccg gcattatacg tgtacaaacc
      241 gccagcctgg gcgaaccgta ttgctgcggt aatatcgtac atcagaccgt taaaatcctt
      301 gccgtgtggc ggtatacctc ccgctgagac cgctgtcatg gtcaccggag gaaaacctga
      361 atc
//
LOCUS       DAJTNM010000389          363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTNM010000389 DAJTNM010000000
VERSION     DAJTNM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.400"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..351
                     /locus_tag="OO172_004369"
     CDS             <1..351
                     /locus_tag="OO172_004369"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HCR5514247.1"
                     /translation="GVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTG
                     NPEVKFLHCLPAFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHT
                     IKAVMVATLSKLNN"
BASE COUNT          106 a           73 c           98 g           86 t
ORIGIN      
        1 ggagttgaag gtgctgactt tatctatacc gatgtatggg tgtcgatggg tgaagcaaaa
       61 gaaaaatggg ctgaacgcat tgcattactg cgtgattatc aggtgaacag caagatgatg
      121 cagttgaccg gtaacccgga ggtcaaattc ctccactgcc tgcccgcgtt tcatgacgac
      181 caaacgacgc ttggcaaaaa aatggcggaa gagtttggcc tacatggcgg aatggaagtg
      241 actgatgagg tcttcgagtc tgccgccagc attgtatttg atcaggcgga aaaccgcatg
      301 cataccatca aagcggtgat ggtcgcgacg ctcagtaaat taaataatta atatctctat
      361 aaa
//
LOCUS       DAJTNM010000390          359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTNM010000390 DAJTNM010000000
VERSION     DAJTNM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.401"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..>359)
                     /locus_tag="OO172_004370"
     CDS             complement(<1..>359)
                     /locus_tag="OO172_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514248.1"
                     /translation="MDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTK
                     REEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAM
                     QITWRGMYQRLLKVDPL"
BASE COUNT           82 a          113 c           67 g           97 t
ORIGIN      
        1 ttcagcgggt caaccttcag taacctctga tacatccctc tccaggtgat ttgcatcgcc
       61 gtttttctca ctgtctccgt tatgatgtac accacttctt ccagtaaccg ccgtttcgcc
      121 ggactcaaaa aaccgtagta cctcaccata cggaacccct tatccgccac atgccaggag
      181 aacctttcca tgaactcatc tccactcatc aacaggtatt cttcccgttt tgttcggtga
      241 ctgttgtaac gcagaccgat ttcatcctga ccggcataat gctccagacg actcatcggc
      301 accggtggct ttttcaggta agagccaaag tacaccgcca catgggtggc attatccat
//
LOCUS       DAJTNM010000391          354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTNM010000391 DAJTNM010000000
VERSION     DAJTNM010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.402"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..172
                     /locus_tag="OO172_004371"
     CDS             <1..172
                     /locus_tag="OO172_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514249.1"
                     /translation="TGITKDAIRKDMVPALRELMQAKHRAQKAVDELVDCVAELETRV
                     GKFEKLVQEALR"
     gene            267..>354
                     /locus_tag="OO172_004372"
     CDS             267..>354
                     /locus_tag="OO172_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5514250.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCN"
BASE COUNT          103 a           74 c          102 g           75 t
ORIGIN      
        1 aacgggcata acaaaagatg ctatccgtaa agatatggta cccgcgcttc gggaactgat
       61 gcaggcgaag catcgcgcac agaaagcagt agatgagctg gtggactgtg tggcagagct
      121 ggaaaccaga gtcggaaagt ttgaaaaact ggtgcaggag gcgctgcgct gatgcgccat
      181 gagtttgttt tgccctggct tgtgcgcgat cattatgtcc ctggtggaaa gcccgggata
      241 aaaatcaccg aaccggagag tatttgatga gcgtcaaaat tcaaacgata ccggaactgc
      301 ttatccagac acgaggtaat atgaccgaag tttcacgaat gctgaactgt aatc
//
LOCUS       DAJTNM010000392          346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTNM010000392 DAJTNM010000000
VERSION     DAJTNM010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.405"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            24..100
                     /locus_tag="OO172_004373"
     tRNA            24..100
                     /locus_tag="OO172_004373"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="OO172_004374"
     tRNA            143..218
                     /locus_tag="OO172_004374"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           98 a           72 c           87 g           89 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg agagttgttc gtgagt
//
LOCUS       DAJTNM010000393          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTNM010000393 DAJTNM010000000
VERSION     DAJTNM010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.406"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="OO172_004375"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="OO172_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR5514251.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           91 a           81 c          100 g           73 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatg
//
LOCUS       DAJTNM010000394          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTNM010000394 DAJTNM010000000
VERSION     DAJTNM010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.407"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>344
                     /locus_tag="OO172_004376"
     CDS             <1..>344
                     /locus_tag="OO172_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514252.1"
                     /translation="WIRNTDWAKIGFKNDSDADTDSYMWFETGDNGNEYFKWRHRLAG
                     GQLKELMNLKWDSLNILVNAVINGCLGIGTTNALGGNSIAFGDNDTGLKQNGDGLLDV
                     YANGQHVFRFQNG"
BASE COUNT           95 a           61 c           94 g           94 t
ORIGIN      
        1 tggattcgga atactgactg ggcgaagatt ggatttaaaa atgattcgga tgcagacacc
       61 gattcctaca tgtggtttga aacaggtgac aacggcaatg agtatttcaa atggagacac
      121 cgcctcgctg gcggccagct caaagagctg atgaatctta aatgggattc actaaatatt
      181 ctggttaatg ccgtcattaa tggttgcctt ggcattggta cgacgaatgc gttaggtgga
      241 aattcaattg ctttcgggga taacgatacc ggcctgaaac agaacggcga tggtctgctg
      301 gatgtttatg cgaatggaca gcatgtattt cgttttcaga atgg
//
LOCUS       DAJTNM010000395          343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTNM010000395 DAJTNM010000000
VERSION     DAJTNM010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.408"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>343
                     /locus_tag="OO172_004377"
     CDS             <1..>343
                     /locus_tag="OO172_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCR5514253.1"
                     /translation="TLGNEAFNRELAKLNQSLRETLSKFNKTTSKNNYSENASNSLDV
                     LATSAIKAGTAIYGFQTALEAYRKVMEIGLKKEASQRAAQFVLGDEGAKRATEFVKNL
                     ADSSGVDQIETF"
BASE COUNT          122 a           53 c           73 g           95 t
ORIGIN      
        1 gacacttgga aacgaagctt ttaatcgtga gttagcaaaa ttaaatcaat ctcttcgtga
       61 aacactttct aagtttaata agaccaccag caaaaataat tacagtgaaa atgcttcaaa
      121 tagtctggat gtattagcta caagtgcaat caaagcaggt acagccattt atggttttca
      181 gacagcctta gaagcttata ggaaggttat ggagattggt ctgaaaaaag aagcatcaca
      241 acgagcggca caattcgttt taggtgatga aggtgctaaa cgagctacag aatttgttaa
      301 gaatcttgct gatagtagtg gtgtagacca gattgaaaca ttt
//
LOCUS       DAJTNM010000396          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTNM010000396 DAJTNM010000000
VERSION     DAJTNM010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.409"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..>334)
                     /locus_tag="OO172_004378"
     CDS             complement(<1..>334)
                     /locus_tag="OO172_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="starvation-sensing protein; maybe involved in
                     homoserine lactone degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional D-altronate/D-mannonate
                     dehydratase"
                     /protein_id="HCR5514254.1"
                     /translation="PIAVGEVFNSIWDCKQLIEEQLIGYIRTTITHAGGITGMRRIAD
                     FASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFE
                     EGYMHPGDE"
BASE COUNT           89 a           95 c           78 g           72 t
ORIGIN      
        1 ttcatcgcct gggtgcatat aaccttcctc gaagcgccag ctgtgcggga aaacttcaag
       61 catttgttcg gaatagccca tgtactcctg aacgccaaaa tttggtaccc ataggtcaaa
      121 atgcagcgcc gccgcatgac aaacaggcga taaatctgaa gggccatgcg aaccggtacg
      181 tacctgataa agcgatgcaa aatcggcaat ccgccgcatc ccggtaatgc cgcctgcatg
      241 ggtgatcgtc gtgcgaatat agccaatcag ctgctcttcg attaactgct tgcagtccca
      301 gatactgtta aacacttcac cgaccgcaat cggc
//
LOCUS       DAJTNM010000397          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTNM010000397 DAJTNM010000000
VERSION     DAJTNM010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.410"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>332
                     /locus_tag="OO172_004379"
     CDS             <1..>332
                     /locus_tag="OO172_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5514255.1"
                     /translation="SKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDR
                     EALHWAVTTGGFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFAR
                     MLGLEPKS"
BASE COUNT           78 a           79 c          111 g           64 t
ORIGIN      
        1 catcgaaacg ggcacatacc ggcagagtgg ctgtgaaaga aagtaatcag cgatggtgct
       61 ctgacgggtt cgagttccgc tgtgataacg gagaaaaact gcgagtcacg ttcgcgctgg
      121 actgctgtga ccgtgaggca ctgcactggg cggtcactac gggcggcttc aacagtgaaa
      181 cagtacagga cgtcatgctg ggagcggtgg aacgccgctt cggcaacgag cttccggcgt
      241 ctccagtaga gtggctgacg gataatggtt catgctaccg ggctaatgaa acacggcagt
      301 ttgcccggat gttggggctt gaaccgaaga gc
//
LOCUS       DAJTNM010000398          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTNM010000398 DAJTNM010000000
VERSION     DAJTNM010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.411"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>327
                     /locus_tag="OO172_004380"
     CDS             <1..>327
                     /locus_tag="OO172_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5514256.1"
                     /translation="TREEAASYLKVDKGTITQWIRSGRLQAVKINPDKPKSPYRICKS
                     DCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLKHRTKGK
                     RKNFTIV"
BASE COUNT           99 a           73 c           89 g           66 t
ORIGIN      
        1 actcgtgaag aggcagcgtc gtatctgaag gtagacaaag gcactatcac gcagtggata
       61 cgaagtggac gacttcaggc cgtaaagata aatccagata aacctaaaag cccatatcgc
      121 atttgcaagt cagactgcat tgcggcgctt aagtctgtga gacacaatag cgcggtgaat
      181 gcggttgatg tgcaggaggt taaagcatgt caatcaaact acgcggtggc acgtggcact
      241 gcgatttcgt cgcgccagat ggatcaagag ttagacgctc tcttgaaaca tcggacaaaa
      301 ggcaagcgca agaacttcac gatcgtc
//
LOCUS       DAJTNM010000399          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTNM010000399 DAJTNM010000000
VERSION     DAJTNM010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.412"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
BASE COUNT          108 a           59 c           60 g          100 t
ORIGIN      
        1 gtttaagctc cattttcaaa ataatatatc agggtttgtt actaataatt tctggaaaca
       61 gaaaaagtag agcagtaatt ctatttcaga gaggagtaac tccatctatc ctcgatacat
      121 actgaacgtt atcgtgatat atttatcatg cgttcttcac cattattcga gtgcagggac
      181 gcaatgacct taaatacctt ttagtagaat atattgctga cccacaaata tcaggatggt
      241 attgacaagt catagatgga gaaatatatg actgaattac cattctctgg aataacagca
      301 gactccttcc catagacggc ttagggg
//
LOCUS       DAJTNM010000400          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTNM010000400 DAJTNM010000000
VERSION     DAJTNM010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.413"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            101..>322
                     /locus_tag="OO172_004381"
     CDS             101..>322
                     /locus_tag="OO172_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5514257.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTR"
BASE COUNT           74 a           79 c           65 g          104 t
ORIGIN      
        1 tttcgctata cccctttata gattctgaat tctcacctat cggctgtttt ctcctcatct
       61 catgtgacat attcccatac tttactcaag gaagattgtc atgaaagcac ctgtttcatt
      121 aacctcattt cgcccacaaa aatctcttgc tatcgccatt ggcgtgcttg ccgtggttgt
      181 gctgccattt ctctcgtatt acaccgttaa tgaaggtgaa cgcggcattt tgctgcgcta
      241 cggaaaaatc gtaaaagttg ccgagccggg gctgggtttt aagatccctt ttatggaaag
      301 cgtggagaaa atttccacgc gt
//
LOCUS       DAJTNM010000401          317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTNM010000401 DAJTNM010000000
VERSION     DAJTNM010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.414"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>317
                     /locus_tag="OO172_004382"
     CDS             <1..>317
                     /locus_tag="OO172_004382"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5514258.1"
                     /translation="GTDAVMLSGESAKGKYPLEAVSIMATICERTDRVMNSRLEFNND
                     NRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFPDATILALTTNEKTA
                     HQL"
BASE COUNT           81 a           88 c           83 g           65 t
ORIGIN      
        1 cggtactgac gcagtgatgc tgtctggtga atccgcaaaa ggtaaatacc cgctggaagc
       61 ggtttctatc atggcgacca tctgcgaacg taccgaccgc gtgatgaaca gccgtctcga
      121 gttcaacaat gacaaccgta aactgcgcat caccgaagcg gtatgccgtg gtgccgttga
      181 aactgctgaa aaactggatg ctccgctgat cgtggttgct acccagggcg gtaaatctgc
      241 tcgcgcagta cgtaaatact tcccggatgc caccatcctg gcactgacca ccaacgaaaa
      301 aacggctcat cagttgg
//
LOCUS       DAJTNM010000402          315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTNM010000402 DAJTNM010000000
VERSION     DAJTNM010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.415"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            71..298
                     /locus_tag="OO172_004383"
     CDS             71..298
                     /locus_tag="OO172_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5514259.1"
                     /translation="MLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGLCANPACI
                     AYAPAIPDIAMMIANKLENFGVTSDERLPDF"
BASE COUNT          106 a           57 c           81 g           71 t
ORIGIN      
        1 aacttttgac gaaaaaaaac cagatgaagc acaggaagaa gagccgaaga aaacggaaaa
       61 agctgatgat ttgttaccgg agaacgctgg cagcgatcag cataatgata gcaataatga
      121 aactggcgaa gatgaactca actggaaaaa acaggtgttg attgcggcgg tttacggttt
      181 atgtgcgaat cctgcgtgca tagcctatgc gcctgcaatc cctgacatcg caatgatgat
      241 tgccaacaag cttgaaaatt ttggagtgac cagtgatgag cgcttacccg atttttgatc
      301 gtattgaaga aatgg
//
LOCUS       DAJTNM010000403          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTNM010000403 DAJTNM010000000
VERSION     DAJTNM010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.417"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>312
                     /locus_tag="OO172_004384"
     CDS             <1..>312
                     /locus_tag="OO172_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5514260.1"
                     /translation="LPDLPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPL
                     PQNLRLLNVGENQLHRLPELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLS
                     TR"
BASE COUNT           98 a           86 c           46 g           82 t
ORIGIN      
        1 ctacctgatc ttcccccggc tctacaaaaa ctttctgttg gcaacaacca gcttactgcc
       61 ttaccagaat taccatgtga actacaggaa ctaagtgctt ttgataacag attacaagag
      121 ctaccgcccc ttcctcaaaa tctgaggctt ttaaacgttg gggaaaacca actacacaga
      181 ctgcccgaac ttccacaacg tctgcaatca ctatatatcc ctaacaatca gctgaacaca
      241 ttgccagaca gtatcatgaa tctgcacatt tatgcagatg ttaatattta taacaatcca
      301 ttgtcgactc gc
//
LOCUS       DAJTNM010000404          311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTNM010000404 DAJTNM010000000
VERSION     DAJTNM010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.418"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..287
                     /locus_tag="OO172_004385"
     CDS             <1..287
                     /locus_tag="OO172_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514261.1"
                     /translation="VGLSGMFGKGGYMDGPGGNVTPVTINGTVWLQGDGCKANTCGWD
                     FIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVKNYVAAVN"
BASE COUNT           78 a           71 c           86 g           76 t
ORIGIN      
        1 cggtcggact gagcggcatg ttcggtaaag gcgggtatat ggatggccct ggcggaaacg
       61 taacgccagt aaccattaac ggtacagtct ggctccaggg cgacggttgc aaagccaata
      121 cctgcggctg ggactttatc gtaacactct ataacccaaa aacccatgaa gtcgttggct
      181 accgctactt tggtttagat gacccggcct acctggtttg gttcggcgaa attggcgtgc
      241 atgaattcgc gtatctggtg aaaaactacg tagctgcggt taactaattt aaggagtctg
      301 tcatgaaaac t
//
LOCUS       DAJTNM010000405          311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTNM010000405 DAJTNM010000000
VERSION     DAJTNM010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.419"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..>311)
                     /locus_tag="OO172_004386"
     CDS             complement(<1..>311)
                     /locus_tag="OO172_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5514262.1"
                     /translation="LLLPATPESCDFLHQIKIGEWIHADFKRVRNYAFHKRFFKLLQL
                     GFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQYLNTVATCRTRDT
                     A"
BASE COUNT           74 a           81 c           77 g           79 t
ORIGIN      
        1 tgccgtatcc cgggttctgc atgtcgcaac tgtattaagg tattgctctg cggcatcact
       61 cagggctgga gtgtgttccc gacctactga ttcgcacagg taatcaacga agcctgatac
      121 cagttttcgt tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat caaatcccag
      181 ttgcaggagt ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc
      241 gtgtatccac tcacctattt tgatttgatg caaaaaatcg caactctccg gcgtcgccgg
      301 gagaagtaat c
//
LOCUS       DAJTNM010000406          308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTNM010000406 DAJTNM010000000
VERSION     DAJTNM010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.420"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..216
                     /locus_tag="OO172_004387"
     CDS             <1..216
                     /locus_tag="OO172_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5514263.1"
                     /translation="EEKIRDAVDEGIQVIEQKTTEPWLDNVYRPVGNVMTVQQQEYEE
                     EHDEESERNFRRGLQKLIAMQDDDPLA"
BASE COUNT          114 a           58 c           75 g           61 t
ORIGIN      
        1 gaagaaaaaa tccgtgatgc cgtggatgaa ggtattcagg ttattgaaca aaaaacgaca
       61 gaaccctggc tggataatgt ttatcgaccc gttggcaatg tgatgaccgt tcagcaacag
      121 gaatatgaag aggagcacga cgaagaatcc gaacgcaatt tcaggcgggg attacagaaa
      181 ctcattgcca tgcaggacga tgacccactg gcctgaataa aaaaacagtc cgcagcaacg
      241 gactgttaaa caggtaaata aagctgattt gaaataaccg aggaaacaat atgaccgata
      301 ttaatgat
//
LOCUS       DAJTNM010000407          306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTNM010000407 DAJTNM010000000
VERSION     DAJTNM010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.421"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..187)
                     /locus_tag="OO172_004388"
     CDS             complement(<1..187)
                     /locus_tag="OO172_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514264.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTIT"
     gene            258..>306
                     /gene="yobI"
                     /locus_tag="OO172_004389"
     CDS             258..>306
                     /gene="yobI"
                     /locus_tag="OO172_004389"
                     /inference="COORDINATES: protein motif:HMM:NF038179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5514265.1"
                     /translation="MYIFITHFFTEYVIFK"
BASE COUNT          100 a           55 c           54 g           97 t
ORIGIN      
        1 tggtgatggt tgaaagggga acccggcttt ctgcaaccag gaagtcttca ccgttatgac
       61 cgataatgat cccgacgtga ttactccagc aatttgatgc agctgatatt tgaccaaata
      121 aggcagcacc tatacatgta aagacgatat ctccaatttc atattcagca gggtaattaa
      181 tattcacgtt gtcattccat tgaaatagat acgaatatac gtaatagcat aaaaaaatta
      241 ttttttcagg agtcgcaatg tatattttta ttacgcattt cttcactgaa tatgtaatat
      301 tcaaat
//
LOCUS       DAJTNM010000408          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTNM010000408 DAJTNM010000000
VERSION     DAJTNM010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.422"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            29..>304
                     /locus_tag="OO172_004390"
     CDS             29..>304
                     /locus_tag="OO172_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCR5514266.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVE"
BASE COUNT           92 a           65 c           62 g           85 t
ORIGIN      
        1 ttcttgatta aataaaatgg agttttacat gaaggctttc aataagctgt tttccctcgt
       61 tgttgcatct gttctggttt tctctcttgc tggctgcggt gacaaagaag aatcgaagaa
      121 attcagcgcc aatctgaacg gcactgaaat tgccattacc tatgtctaca aaggtgacaa
      181 ggtgcttaag caatcttctg aaaccaaaat tcaatttgcc tccattggtg caaccaccaa
      241 agaagatgct gccaagacac ttgagccgtt aagcgccaaa tacaaaaaca tcgcgggtgt
      301 tgaa
//
LOCUS       DAJTNM010000409          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTNM010000409 DAJTNM010000000
VERSION     DAJTNM010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.denovo.423"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..>303
                     /locus_tag="OO172_004391"
     CDS             <1..>303
                     /locus_tag="OO172_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5514267.1"
                     /translation="VTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLP
                     SSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYIS"
BASE COUNT           72 a           73 c           99 g           59 t
ORIGIN      
        1 gtcacgttcg cgctggactg ctgtgatcgt gaggcactgc actgggcggt gactaccggc
       61 ggcttcaaca gtgaaacagt acaggacgtc atgctgggag cggtggaacg ccgcttcggc
      121 aacgatcttc cgtcgtctcc agtggagtgg ctgacggata atggttcatg ctaccgggct
      181 aatgaaacac gccagttcgc ccggatgttg ggacttgaac cgaagaacac ggcggtgcgg
      241 agtccggaga gtaacggaat agcagagagc ttcgtgaaaa cgataaagcg tgactacatc
      301 agt
//
LOCUS       DAJTNM010000410         4952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.guided.202, whole genome shotgun sequence.
ACCESSION   DAJTNM010000410 DAJTNM010000000
VERSION     DAJTNM010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.guided.202"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            575..1390
                     /gene="sul2"
                     /locus_tag="OO172_004392"
     CDS             575..1390
                     /gene="sul2"
                     /locus_tag="OO172_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5514268.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1477..1779
                     /locus_tag="OO172_004393"
     CDS             1477..1779
                     /locus_tag="OO172_004393"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514269.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(1955..3448)
                     /locus_tag="OO172_004394"
     CDS             complement(1955..3448)
                     /locus_tag="OO172_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCR5514270.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(3492..3881)
                     /locus_tag="OO172_004395"
     CDS             complement(3492..3881)
                     /locus_tag="OO172_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5514271.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4099..4539
                     /locus_tag="OO172_004396"
     CDS             4099..4539
                     /locus_tag="OO172_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5514272.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1148 a         1381 c         1317 g         1106 t
ORIGIN      
        1 taactatcaa cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat
       61 tagtaacagg agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg
      121 gctacactga aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag
      181 cagttcccca actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta
      241 cagagcaaga gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta
      301 tcaattaatc ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa
      361 caggtttccc tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct
      421 acttattgag agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg
      481 atcaccgcaa acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga
      541 ctcatgctcg attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg
      601 catcgtcaac ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc
      661 agccattgcg caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc
      721 ggcatccagc aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc
      781 gccggtgctg gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc
      841 cgcgacgcaa gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt
      901 tccagacgct gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca
      961 ttcggtgcaa gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca
     1021 cattgcggcg ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa
     1081 ccgccttgtc cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct
     1141 ctcggtgctg gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc
     1201 tgtttcgcgc aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc
     1261 cgcgacactc gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca
     1321 cgagccgcgc cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag
     1381 aattcgttaa ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc
     1441 ctttgagggc gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca
     1501 gacggtattc gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc
     1561 ggcatggcgg ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc
     1621 aaggatacgc gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg
     1681 atgggcatgg acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc
     1741 ccttttcacc caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat
     1801 gcaacccaat ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca
     1861 ctagcggctc cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca
     1921 gcattttgat tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct
     1981 cagccggtgg cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct
     2041 tcaggtgatc aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca
     2101 tggcgccgcc gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt
     2161 gcgcccatgt catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga
     2221 ccttgttgcc cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg
     2281 cgaacacccc gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc
     2341 ttgcaatgaa atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct
     2401 tgagctggta gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg
     2461 ggcggctgat gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca
     2521 cgccggcgtg caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac
     2581 tggtcggcag agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac
     2641 ggtaagtgat cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg
     2701 ccagatagct gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca
     2761 cccggtgggc gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc
     2821 agcggaaacg cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt
     2881 ggaagtgaac attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg
     2941 tcttggccac ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt
     3001 aaacgatgcc cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct
     3061 gaaacgggaa gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca
     3121 gcaaggcggc actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct
     3181 tacagctgaa agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc
     3241 catgctccag ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca
     3301 attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct
     3361 gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc
     3421 tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata
     3481 cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga
     3541 cattgccagc cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg
     3601 ttattgcgtg caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa
     3661 ttaatattct tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat
     3721 gcagtataag atgaaccact actattatca tggccaccat taccaccacc gttaaaacta
     3781 cctcccgaac ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga
     3841 ccaaaaccac cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact
     3901 tttaaaaaca gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact
     3961 acgcccgtca aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca
     4021 gcctcagacc ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca
     4081 ggagacactg tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga
     4141 aagtcgcgct cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga
     4201 aaaaatgatc gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc
     4261 acttctgaga caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg
     4321 gaaaatgctc agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc
     4381 ctaatgcttg aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg
     4441 gaacaactac aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg
     4501 ggattctggt cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt
     4561 accataacat ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac
     4621 cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta
     4681 cggcatgcat gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg
     4741 taagtcctgt ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg
     4801 cgtggccgtg aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg
     4861 gggtgatgta caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa
     4921 aagtgatgta taagtgatgt aaaagtgatg ta
//
LOCUS       DAJTNM010000411         4045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.guided.213, whole genome shotgun sequence.
ACCESSION   DAJTNM010000411 DAJTNM010000000
VERSION     DAJTNM010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.guided.213"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..1371)
                     /gene="pepD"
                     /locus_tag="OO172_004397"
     CDS             complement(<1..1371)
                     /gene="pepD"
                     /locus_tag="OO172_004397"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5514273.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPH"
     gene            1633..2091
                     /gene="gpt"
                     /locus_tag="OO172_004398"
     CDS             1633..2091
                     /gene="gpt"
                     /locus_tag="OO172_004398"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5514274.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2183..3427
                     /gene="frsA"
                     /locus_tag="OO172_004399"
     CDS             2183..3427
                     /gene="frsA"
                     /locus_tag="OO172_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5514275.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3485..3886
                     /gene="crl"
                     /locus_tag="OO172_004400"
     CDS             3485..3886
                     /gene="crl"
                     /locus_tag="OO172_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5514276.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(3925..>4045)
                     /locus_tag="OO172_004401"
     CDS             complement(3925..>4045)
                     /locus_tag="OO172_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5514277.1"
                     /translation="YFNKNMSAFVDYKINKLDSDNKLNINNDDTVAVGMTYQF"
BASE COUNT          973 a         1002 c         1045 g         1025 t
ORIGIN      
        1 gtgtggaccg gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt
       61 gaacagacca cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag
      121 gcgctgatag gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc
      181 aggatatgcg cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat
      241 gctcaccacg tagtctttac cgctgtcgat cagtgaacgg atcaggcagt gaatttctac
      301 gttattgtca gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc
      361 cacgtcggaa ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc
      421 gcgagatttc gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag
      481 atttttctct ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga
      541 tttcagtgcg tcgactttat cagctgtgac agcaatggtc gcaaaggctt cacgcgggat
      601 ggcgttacgc agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg
      661 acccgccagg aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc
      721 gccggagtga ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg
      781 aaccgcttca cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat
      841 gtagatttca ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt
      901 gctctgtaag ccgaacgcac cgtccatacc ggcttcttcg gttatggtca gcagcacttc
      961 cagcgggccg tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat
     1021 gccgttatcc gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg
     1081 ctggatagga tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc
     1141 gaggtgggcc tgtaagacga ccggtttacg attttccata cctgcggtag caggtttacg
     1201 aatcaggata ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc
     1261 aacaatgtat tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt
     1321 ggcaaaaata tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct
     1381 ccttgtcgat cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca
     1441 acacaagaca ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga
     1501 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatatc
     1561 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
     1621 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
     1681 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
     1741 cgtggcggtc tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat
     1801 accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc
     1861 gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact
     1921 gctgttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg
     1981 gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa
     2041 cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa
     2101 cgcctggcat tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt
     2161 aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc
     2221 ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc
     2281 gcctgtgcag tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg
     2341 tctgatgtgg atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat
     2401 tgtgatgagc gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg
     2461 tggtggcaac tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg
     2521 tacggaagaa gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa
     2581 cattgccgcc tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc
     2641 aaaccgcgcc tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt
     2701 taccgtaccc ggcggtgcgc ccatcaccgg ctttttgcat atgccgcaag gcgatggccc
     2761 gttcccgaca gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct
     2821 gtatgaacgt tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt
     2881 gggcttttct tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt
     2941 aaaggcgctg cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg
     3001 tttcggcgct aacgttgccg tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt
     3061 tgcctgtctt ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt
     3121 gccggaaatg tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga
     3181 agcgttacgc gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg
     3241 ctgcccaacg ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga
     3301 ctcacgctta atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc
     3361 ggtgtatcgg aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt
     3421 gtgttaaaaa tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat
     3481 agcaatgacg ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact
     3541 aggcccgtat attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc
     3601 tgtatgcgtc aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga
     3661 gctggaagcg caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc
     3721 aggcgactgg aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac
     3781 cctgcgtgag tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga
     3841 accggcggat gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg
     3901 atgcatcaca tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc
     3961 atcattatta atattcagct tattatcgct atccagtttg ttgattttat aatcaacaaa
     4021 cgctgacata tttttgttga aataa
//
LOCUS       DAJTNM010000412         2403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.guided.238, whole genome shotgun sequence.
ACCESSION   DAJTNM010000412 DAJTNM010000000
VERSION     DAJTNM010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.guided.238"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..110)
                     /locus_tag="OO172_004402"
     CDS             complement(<1..110)
                     /locus_tag="OO172_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR5514278.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGV"
     gene            192..1397
                     /gene="tet(B)"
                     /locus_tag="OO172_004403"
     CDS             192..1397
                     /gene="tet(B)"
                     /locus_tag="OO172_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5514279.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1510..2103)
                     /gene="tetC"
                     /locus_tag="OO172_004404"
     CDS             complement(1510..2103)
                     /gene="tetC"
                     /locus_tag="OO172_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5514280.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2191..2367
                     /locus_tag="OO172_004405"
                     /pseudo
     CDS             2191..2367
                     /locus_tag="OO172_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          640 a          426 c          493 g          844 t
ORIGIN      
        1 tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta
       61 agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat cattaattcc
      121 taatttttgt tgacactcta tcattgatag agttatttta ccactcccta tcagtgatag
      181 agaaaagtga aatgaatagt tcgacaaaga tcgcattggt aattacgtta ctcgatgcca
      241 tggggattgg ccttatcatg ccagtcttgc caacgttatt acgtgaattt attgcttcgg
      301 aagatatcgc taaccacttt ggcgtattgc ttgcacttta tgcgttaatg caggttatct
      361 ttgctccttg gcttggaaaa atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt
      421 cattaatagg cgcatcgctg gattacttat tgctggcttt ttcaagtgcg ctttggatgc
      481 tgtatttagg ccgtttgctt tcagggatca caggagctac tggggctgtc gcggcatcgg
      541 tcattgccga taccacctca gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa
      601 gttttgggct tggtttaata gcggggccta ttattggtgg ttttgcagga gagatttcac
      661 cgcatagtcc cttttttatc gctgcgttgc taaatattgt cgctttcctt gtggttatgt
      721 tttggttccg tgaaaccaaa aatacacgtg ataatacaga taccgaagta ggggttgaga
      781 cgcaatcgaa ttcggtatac atcactttat ttaaaacgat gcccattttg ttgattattt
      841 atttttcagc gcaattgata ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa
      901 atcgttttgg atggaatagc atgatggttg gcttttcatt agcgggtctt ggtcttttac
      961 actcagtatt ccaagccttt gtggcaggaa gaatagccac taaatggggc gaaaaaacgg
     1021 cagtactgct cggatttatt gcagatagta gtgcatttgc ctttttagcg tttatatctg
     1081 aaggttggtt agttttccct gttttaattt tattggctgg tggtgggatc gctttacctg
     1141 cattacaggg agtgatgtct atccaaacaa agagtcatca gcaaggtgct ttacagggat
     1201 tattggtgag ccttaccaat gcaaccggtg ttattggccc attactgttt gctgttattt
     1261 ataatcattc actaccaatt tgggatggct ggatttggat tattggttta gcgttttact
     1321 gtattattat cctgctatcg atgaccttca tgttaacccc tcaagctcag gggagtaaac
     1381 aggagacaag tgcttagtta tttcgtcacc aaatgatgtt attccgcgaa atataatgac
     1441 cctcttgata acccaagagg gcatttttta cgataaagaa gatttagctt caaataaaac
     1501 ctatctattt tatttatctt tcaagctcaa taaaaagccg cggtaaatag caataaattg
     1561 gcctttttta tcggcaagct cttttaggtt tttcgcatgt attgcgatat gcataaacca
     1621 gccattgagt aagtttttaa gcacatcatc atcataagct ttaagttggt tctcttggat
     1681 caatttgctg acaatggcgt ttaccttacc agtaatgtat tcaaggctaa ttttttcaag
     1741 ttcattccaa ccaatgatag gcatcacttc ttggataggg ataaggtttt tattattatc
     1801 aataatataa tcaagataat gttcaaatat actttctaag gcagaccaac catttgttaa
     1861 atcagttttt gttgtgatgt aggcatcaat cataattaat tgctgcttat aacaggcact
     1921 gagtaattgt tttttatttt taaagtgatg ataaaaggca cctttggtca ccaacgcttt
     1981 tcccgagatc tcatctattg aaacagcttg atagcctttt tcaacaaaca atattcgtgc
     2041 tgagttaacc agtgattgat aggtactctt aaaattttct tgttgatgat ttttattttc
     2101 catgatagat ttaaaataac ataccgtcag tatgtttatg gtatcatgat gatgtggtcg
     2161 tgacaatctt aagaacattt aggttatttt atgtatattg aacagcattc tcgctatcaa
     2221 aataaagcta ataacatcca attaagatat gatgataagc agtttcatac aacggttatc
     2281 aaagatgttc tattatggat tgaacataat ttagatcagt ctttactgct tgatgatgtg
     2341 gcgaataaag cgggttatac caagtgggta atgactccaa cttactgata gtgttttatg
     2401 ttc
//
LOCUS       DAJTNM010000413         2231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.guided.240, whole genome shotgun sequence.
ACCESSION   DAJTNM010000413 DAJTNM010000000
VERSION     DAJTNM010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.guided.240"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..237
                     /locus_tag="OO172_004406"
     CDS             <1..237
                     /locus_tag="OO172_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HCR5514281.1"
                     /translation="PEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANI
                     IVKDEKMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            248..607
                     /gene="frdD"
                     /locus_tag="OO172_004407"
     CDS             248..607
                     /gene="frdD"
                     /locus_tag="OO172_004407"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5514282.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            670..1803
                     /locus_tag="OO172_004408"
     CDS             670..1803
                     /locus_tag="OO172_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5514283.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            1892..>2231
                     /gene="blc"
                     /locus_tag="OO172_004409"
     CDS             1892..>2231
                     /gene="blc"
                     /locus_tag="OO172_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5514284.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFY"
BASE COUNT          540 a          569 c          571 g          551 t
ORIGIN      
        1 ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac
       61 ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa
      121 gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc
      181 tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga
      241 gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt
      301 cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat
      361 tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt
      421 cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg
      481 tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa
      541 atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac
      601 aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa
      661 ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca
      721 tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag
      781 caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt
      841 acttggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag
      901 ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg
      961 gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg
     1021 aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg
     1081 ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca
     1141 tgggccccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg
     1201 gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca
     1261 ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg
     1321 ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat
     1381 ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt accccccctt
     1441 gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa
     1501 accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac
     1561 agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt
     1621 aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga
     1681 tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac
     1741 aaaaactatc ccaatccaga gagagtcgcc gccgcctgtc agattctcaa cgctctacag
     1801 taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta
     1861 cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc
     1921 gacagctgca tttctggtcg ttgcctgtag ttctcctacg ccgccgcgtg gcgtgaccgt
     1981 agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga
     2041 tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg
     2101 cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga
     2161 agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg
     2221 tcctttctat g
//
LOCUS       DAJTNM010000414         1926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.guided.251, whole genome shotgun sequence.
ACCESSION   DAJTNM010000414 DAJTNM010000000
VERSION     DAJTNM010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.guided.251"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            complement(<1..642)
                     /gene="intI1"
                     /locus_tag="OO172_004410"
     CDS             complement(<1..642)
                     /gene="intI1"
                     /locus_tag="OO172_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5514285.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPD"
     gene            802..1275
                     /locus_tag="OO172_004411"
     CDS             802..1275
                     /locus_tag="OO172_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5514286.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1368..>1926
                     /locus_tag="OO172_004412"
     CDS             1368..>1926
                     /locus_tag="OO172_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR5514287.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNV"
BASE COUNT          491 a          497 c          508 g          430 t
ORIGIN      
        1 gtcgggaagc gcaacgccgc tgcggccctc ggcctggtcc ttcagccacc atgcccgtgc
       61 acgcgacagc tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc
      121 cttggagccc ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac
      181 ccgcagttgc aaaccctcac tgatccgcat gcccgttcca tacagaagct gggcgaacaa
      241 acgatgctcg ccttccagaa aaccgaggat gcgaaccact tcatccgggg tcagcaccac
      301 cggcaagcgc cgcgacggcc gaggtcttcc gatctcctga agccagggca gatccgtgca
      361 cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc tgacgatgcg tggagaccga
      421 aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc tgcccaaggt
      481 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
      541 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
      601 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
      661 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
      721 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
      781 gttaacctct gaggaagaat tgtgaaacta tcactaatgg tagctatatc gaagaatgga
      841 gttatcggga atggccctga tattccatgg agtgccaaag gtgaacagct cctgtttaaa
      901 gctattacct ataaccaatg gctgttggtt ggacgcaaga cttttgaatc aatgggagca
      961 ttacccaacc gaaagtatgc ggtcgtaaca cgttcaagtt ttacatctga caatgagaac
     1021 gtagtgatct ttccatcaat taaagatgct ttaaccaacc taaagaaaat aacggatcat
     1081 gtcattgttt caggtggtgg ggagatatac aaaagcctga tcgatcaagt agatacacta
     1141 catatatcta caatagacat cgagccggaa ggtgatgttt actttcctga aatccccagc
     1201 aattttaggc cagtttttac ccaagacttc gcctctaaca taaattatag ttaccaaatc
     1261 tggcaaaagg gttaacaagt ggcagcaacg gattcgcaaa cctgtcacgc cttttgtacc
     1321 aaaagccgcg ccaggtttgc gatccgctgt gccaggcgtt aaacatcatg agggaagcgg
     1381 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     1441 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     1501 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     1561 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     1621 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     1681 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     1741 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     1801 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     1861 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     1921 atgtag
//
LOCUS       DAJTNM010000415         1354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.guided.273, whole genome shotgun sequence.
ACCESSION   DAJTNM010000415 DAJTNM010000000
VERSION     DAJTNM010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.guided.273"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            263..922
                     /locus_tag="OO172_004413"
     CDS             263..922
                     /locus_tag="OO172_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5514288.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..>1354)
                     /locus_tag="OO172_004414"
     CDS             complement(1123..>1354)
                     /locus_tag="OO172_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5514289.1"
                     /translation="ELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGA
                     NRFYQALGGRINDHIVNYYLHGESLSKLASGI"
BASE COUNT          359 a          302 c          317 g          376 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctct
//
LOCUS       DAJTNM010000416         1315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702761
            SAMEA8900950-rid15820613.guided.275, whole genome shotgun sequence.
ACCESSION   DAJTNM010000416 DAJTNM010000000
VERSION     DAJTNM010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900950
            Sequence Read Archive: ERR6005312
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478226.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478226.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,416
            CDSs (total)                      :: 4,338
            Genes (coding)                    :: 3,956
            CDSs (with protein)               :: 3,956
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 130 of 382
            Pseudo Genes (multiple problems)  :: 40 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900950-rid15820613.guided.275"
                     /serovar="S. flexneri 3a"
                     /isolate="702761"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-10-20"
     gene            <1..311
                     /locus_tag="OO172_004415"
     CDS             <1..311
                     /locus_tag="OO172_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR5514290.1"
                     /translation="NRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGG
                     GEIYKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG
                     "
     gene            404..1195
                     /gene="aadA1"
                     /locus_tag="OO172_004416"
     CDS             404..1195
                     /gene="aadA1"
                     /locus_tag="OO172_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5514291.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          351 a          313 c          335 g          316 t
ORIGIN      
        1 ccaaccgaaa gtatgcggtc gtaacacgtt caagttttac atctgacaat gagaacgtag
       61 tgatctttcc atcaattaaa gatgctttaa ccaacctaaa gaaaataacg gatcatgtca
      121 ttgtttcagg tggtggggag atatacaaaa gcctgatcga tcaagtagat acactacata
      181 tatctacaat agacatcgag ccggaaggtg atgtttactt tcctgaaatc cccagcaatt
      241 ttaggccagt ttttacccaa gacttcgcct ctaacataaa ttatagttac caaatctggc
      301 aaaagggtta acaagtggca gcaacggatt cgcaaacctg tcacgccttt tgtaccaaaa
      361 gccgcgccag gtttgcgatc cgctgtgcca ggcgttaaac atcatgaggg aagcggtgat
      421 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
      481 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
      541 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
      601 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
      661 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
      721 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
      781 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
      841 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
      901 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
      961 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     1021 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     1081 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     1141 ggaagaattt gttcactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     1201 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     1261 taagcacata attgcggtga tgctgccaac ttactgattt agtgtatgat ggtgt
//
